2005 |
Sarropoulou, E; Kotoulas, G; Power, D M; Geisler, R Physiological Genomics, 23 (2), pp. 182–191, 2005, ISSN: 10948341. @article{sarropoulou_gene_2005, title = {Gene expression profiling of gilthead sea bream during early development and detection of stress-related genes by the application of cDNA microarray technology}, author = {E Sarropoulou and G Kotoulas and D M Power and R Geisler}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33745678365&doi=10.1152%2fphysiolgenomics.00139.2005&partnerID=40&md5=497797e3b9a6bc0c8a3554c078ea6805}, doi = {10.1152/physiolgenomics.00139.2005}, issn = {10948341}, year = {2005}, date = {2005-01-01}, journal = {Physiological Genomics}, volume = {23}, number = {2}, pages = {182--191}, abstract = {Large-scale gene expression studies were performed for one of the main European aquaculture species, the gilthead sea bream Sparus auratus L. For this purpose, a cDNA microarray containing 10,176 clones from a cDNA library of mixed embryonic and larval stages was constructed. In addition to its importance for aquaculture, the taxonomic position and the relatively small genome size of sea bream makes it a prospective model for evolutionary biology and comparative genomics. However, so far, no large-scale analysis of gene expression exists for this species. In the present study, gene expression was analyzed in gilthead sea bream during early development, a significant period in the determination of quantitative traits and therefore of considerable interest for aquaculture. Synexpression groups expressed primarily early and late in development were determined and were composed of both known and novel genes. Furthermore, it was possible to identify stress response genes induced by cortisol injections using the cDNA microarray generated. The creation of gene expression profiles for sea bream by microarray hybridization will accelerate identification of candidate genes involved in multifactorial traits and certain regulatory pathways and will also contribute to a better understanding of the genetic background of fish physiology, which may help to improve aquaculture practices. Copyright © 2005 the American Physiological Society.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Large-scale gene expression studies were performed for one of the main European aquaculture species, the gilthead sea bream Sparus auratus L. For this purpose, a cDNA microarray containing 10,176 clones from a cDNA library of mixed embryonic and larval stages was constructed. In addition to its importance for aquaculture, the taxonomic position and the relatively small genome size of sea bream makes it a prospective model for evolutionary biology and comparative genomics. However, so far, no large-scale analysis of gene expression exists for this species. In the present study, gene expression was analyzed in gilthead sea bream during early development, a significant period in the determination of quantitative traits and therefore of considerable interest for aquaculture. Synexpression groups expressed primarily early and late in development were determined and were composed of both known and novel genes. Furthermore, it was possible to identify stress response genes induced by cortisol injections using the cDNA microarray generated. The creation of gene expression profiles for sea bream by microarray hybridization will accelerate identification of candidate genes involved in multifactorial traits and certain regulatory pathways and will also contribute to a better understanding of the genetic background of fish physiology, which may help to improve aquaculture practices. Copyright © 2005 the American Physiological Society. |
Papadaki, M; Piferrer, F; Zanuy, S; Maingot, E; Divanach, P; Mylonas, C C Journal of Fish Biology, 66 (4), pp. 938–956, 2005, ISSN: 00221112, (Publisher: Blackwell Publishing Ltd). @article{papadaki_growth_2005, title = {Growth, sex differentiation and gonad and plasma levels of sex steroids in male- and female-dominant populations of Dicentrarchus labrax obtained through repeated size grading}, author = {M Papadaki and F Piferrer and S Zanuy and E Maingot and P Divanach and C C Mylonas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-17244363508&doi=10.1111%2fj.0022-1112.2005.00639.x&partnerID=40&md5=818526ad19bf4809e7b643ad51c39425}, doi = {10.1111/j.0022-1112.2005.00639.x}, issn = {00221112}, year = {2005}, date = {2005-01-01}, journal = {Journal of Fish Biology}, volume = {66}, number = {4}, pages = {938--956}, abstract = {Starting from 66 days post hatching (dph), European sea bass Dicentrarchus labrax were graded successively to create a fast growing (L-extreme) and a slow growing (S-extreme) population. The L-extreme population grew significantly larger (ANOVA}, note = {Publisher: Blackwell Publishing Ltd}, keywords = {}, pubstate = {published}, tppubtype = {article} } Starting from 66 days post hatching (dph), European sea bass Dicentrarchus labrax were graded successively to create a fast growing (L-extreme) and a slow growing (S-extreme) population. The L-extreme population grew significantly larger (ANOVA |
Bahri-Sfar, Lilia; Lemaire, Christophe; Chatain, Béatrice; Divanach, Pascal; Hassine, Oum Kalthoum Ben; Bonhomme, François Impact of aquaculture on the genetic structure of mediterranean populations of Dicentrarchus labrax Journal Article Aquatic Living Resources, 18 (1), pp. 71–76, 2005, ISSN: 0990-7440, 1765-2952, (Original title (in French): Impact de l'élevage sur la structure génétique des populations méditerranéennes de Dicentrarchus labrax). @article{bahri-sfar_impact_2005, title = {Impact of aquaculture on the genetic structure of mediterranean populations of Dicentrarchus labrax}, author = {Lilia Bahri-Sfar and Christophe Lemaire and Béatrice Chatain and Pascal Divanach and Oum Kalthoum Ben Hassine and François Bonhomme}, url = {http://www.alr-journal.org/10.1051/alr:2005007}, doi = {10.1051/alr:2005007}, issn = {0990-7440, 1765-2952}, year = {2005}, date = {2005-01-01}, urldate = {2020-08-18}, journal = {Aquatic Living Resources}, volume = {18}, number = {1}, pages = {71--76}, note = {Original title (in French): Impact de l'élevage sur la structure génétique des populations méditerranéennes de Dicentrarchus labrax}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Kokokiris, L; Canario, A; Mylonas, C; Pavlidis, M; Kentouri, M; Divanach, P Induction of ovulation and spawning in the Mediterranean Red Porgy, Pagrus Pagrus, by controlled delivery and acute injection of GnRHa Journal Article Israeli Journal of Aquaculture - Bamidgeh, 57 (4), pp. 223–230, 2005, ISSN: 0792156X. @article{kokokiris_induction_2005, title = {Induction of ovulation and spawning in the Mediterranean Red Porgy, Pagrus Pagrus, by controlled delivery and acute injection of GnRHa}, author = {L Kokokiris and A Canario and C Mylonas and M Pavlidis and M Kentouri and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-29744437550&partnerID=40&md5=fe674a5bea66c6aa820eb3a5b7d62661}, issn = {0792156X}, year = {2005}, date = {2005-01-01}, journal = {Israeli Journal of Aquaculture - Bamidgeh}, volume = {57}, number = {4}, pages = {223--230}, abstract = {Gonadotropin-releasing hormone analogue (GnRHa) in the form of saline injections or sustained-release microspheres was used to induce oocyte maturation, ovulation, and spawning in captive red porgy (Pagrus pagrus). Individually tagged vitellogenic females (n = 9 or 10) were treated at the beginning of the spawning season (March) with 20 μg/kg body weight (bw) GnRHa-loaded microspheres, a single injection of 20 μg/ kg bw dissolved in saline, or physiological saline (control). Females were placed in tanks (one tank per treatment) connected to overflow egg collectors and monitored for 11 days. In addition to the eggs collected from the tank overflow, eggs were stripped from the fish on a daily basis. Only one spawn was obtained from the control fish, probably from a single female, given the small relative fecundity (700 eggs/kg bw). On the contrary, treatment with a GnRHa injection produced two spawns (9 and 11 days after treatment) and 50% of the fish ovulated. Treatment with GnRHa microspheres induced seven spawns (3 and 6-11 days after treatment) and 100% of the females ovulated. Females did not spawn all the eggs ovulated on a particular day, evident from the significant number of eggs obtained by manual stripping. Egg quality did not significantly differ among treatments, whereas number of spawned eggs and total relative fecundity were significantly higher in fish treated with GnRHa microspheres (ANOVA, ptextless0.05). The results demonstrate the potential of GnRHa-loaded microspheres to induce spawning in red porgy, as a method of overcoming spawning failures in commercial hatcheries.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Gonadotropin-releasing hormone analogue (GnRHa) in the form of saline injections or sustained-release microspheres was used to induce oocyte maturation, ovulation, and spawning in captive red porgy (Pagrus pagrus). Individually tagged vitellogenic females (n = 9 or 10) were treated at the beginning of the spawning season (March) with 20 μg/kg body weight (bw) GnRHa-loaded microspheres, a single injection of 20 μg/ kg bw dissolved in saline, or physiological saline (control). Females were placed in tanks (one tank per treatment) connected to overflow egg collectors and monitored for 11 days. In addition to the eggs collected from the tank overflow, eggs were stripped from the fish on a daily basis. Only one spawn was obtained from the control fish, probably from a single female, given the small relative fecundity (700 eggs/kg bw). On the contrary, treatment with a GnRHa injection produced two spawns (9 and 11 days after treatment) and 50% of the fish ovulated. Treatment with GnRHa microspheres induced seven spawns (3 and 6-11 days after treatment) and 100% of the females ovulated. Females did not spawn all the eggs ovulated on a particular day, evident from the significant number of eggs obtained by manual stripping. Egg quality did not significantly differ among treatments, whereas number of spawned eggs and total relative fecundity were significantly higher in fish treated with GnRHa microspheres (ANOVA, ptextless0.05). The results demonstrate the potential of GnRHa-loaded microspheres to induce spawning in red porgy, as a method of overcoming spawning failures in commercial hatcheries. |
Mylonas, C C; Anezaki, L; Divanach, P; Zanuy, S; Piferrer, F; Ron, B; Peduel, A; Atia, Ben I; Gorshkov, S; Tandler, A Journal of Fish Biology, 67 (3), pp. 652–668, 2005, ISSN: 00221112. @article{mylonas_influence_2005, title = {Influence of rearing temperature during the larval and nursery periods on growth and sex differentiation in two Mediterranean strains of Dicentrarchus labrax}, author = {C C Mylonas and L Anezaki and P Divanach and S Zanuy and F Piferrer and B Ron and A Peduel and I Ben Atia and S Gorshkov and A Tandler}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-20244367191&doi=10.1111%2fj.0022-1112.2005.00766.x&partnerID=40&md5=4bcba3bc5b4f5b44ae84310c6b854a28}, doi = {10.1111/j.0022-1112.2005.00766.x}, issn = {00221112}, year = {2005}, date = {2005-01-01}, journal = {Journal of Fish Biology}, volume = {67}, number = {3}, pages = {652--668}, abstract = {European sea bass Dicentrarchus labrax of the north-western (NW) and south-eastern (SE) Mediterranean Sea strains were exposed to different temperatures (13, 17 or 21°C) during the larval rearing (11-51 days post hatching, dph) or nursery periods (55-95 dph), in order to examine the effects of temperature on sex differentiation and subsequent growth during the first year of life. Higher growth was observed during exposure to higher temperatures, but fish of the NW strain exposed to 13 or 17°C during larval rearing exhibited compensatory growth once exposure to the lower temperatures finished, and as a result their final size at 300 dph was similar or greater to the group exposed to 21°C. Fish exposed to 17°C during the nursery period also had similar size to fish exposed to 21°C after 300 days of rearing, but the fish exposed to 13°C remained significantly smaller (ANOVA}, keywords = {}, pubstate = {published}, tppubtype = {article} } European sea bass Dicentrarchus labrax of the north-western (NW) and south-eastern (SE) Mediterranean Sea strains were exposed to different temperatures (13, 17 or 21°C) during the larval rearing (11-51 days post hatching, dph) or nursery periods (55-95 dph), in order to examine the effects of temperature on sex differentiation and subsequent growth during the first year of life. Higher growth was observed during exposure to higher temperatures, but fish of the NW strain exposed to 13 or 17°C during larval rearing exhibited compensatory growth once exposure to the lower temperatures finished, and as a result their final size at 300 dph was similar or greater to the group exposed to 21°C. Fish exposed to 17°C during the nursery period also had similar size to fish exposed to 21°C after 300 days of rearing, but the fish exposed to 13°C remained significantly smaller (ANOVA |
Poulakakis, N; Goulielmos, G; Antoniou, A; Zouros, E; Mylonas, M Isolation and characterization of polymorphic microsatellite markers in the wall lizard Podarcis erhardii (Squamata: Lacertidae) Journal Article Molecular Ecology Notes, 5 (3), pp. 549–551, 2005. @article{poulakakis_isolation_2005, title = {Isolation and characterization of polymorphic microsatellite markers in the wall lizard Podarcis erhardii (Squamata: Lacertidae)}, author = {N Poulakakis and G Goulielmos and A Antoniou and E Zouros and M Mylonas}, doi = {10.1111/j.1471-8286.2005.00986.x}, year = {2005}, date = {2005-01-01}, journal = {Molecular Ecology Notes}, volume = {5}, number = {3}, pages = {549--551}, abstract = {Five polymorphic microsatellite loci (containing di- and penta-nucleotide repeats) were developed for the Erhard's wall lizard, Podarcis erhardii, an endemic species of southern Balkan Peninsula. The number of alleles ranged from five to 17. Observed and expected heterozygosities ranged from 0.28 to 0.92 and 0.57 to 0.91, respectively. These markers will provide a valuable tool for population genetics analyses and can contribute to the evaluation of conservation programs. © 2005 Blackwell Publishing Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Five polymorphic microsatellite loci (containing di- and penta-nucleotide repeats) were developed for the Erhard's wall lizard, Podarcis erhardii, an endemic species of southern Balkan Peninsula. The number of alleles ranged from five to 17. Observed and expected heterozygosities ranged from 0.28 to 0.92 and 0.57 to 0.91, respectively. These markers will provide a valuable tool for population genetics analyses and can contribute to the evaluation of conservation programs. © 2005 Blackwell Publishing Ltd. |
Mantziou, G; Antoniou, A; Poulakakis, N; Goulielmos, G; Tsigenopoulos, C S; Pinou, T; Mylonas, M Isolation and characterization of six polymorphic microsatellite markers in the freshwater turtle Mauremys rivulata (Testudines: Geoemydidae) Journal Article Molecular Ecology Notes, 5 (4), pp. 727–729, 2005. @article{mantziou_isolation_2005, title = {Isolation and characterization of six polymorphic microsatellite markers in the freshwater turtle Mauremys rivulata (Testudines: Geoemydidae)}, author = {G Mantziou and A Antoniou and N Poulakakis and G Goulielmos and C S Tsigenopoulos and T Pinou and M Mylonas}, url = {http://www.blackwell-synergy.com/doi/abs/10.1111/j.1471-8286.2005.01046.x}, doi = {10.1111/j.1471-8286.2005.01046.x}, year = {2005}, date = {2005-01-01}, journal = {Molecular Ecology Notes}, volume = {5}, number = {4}, pages = {727--729}, abstract = {Abstract Six polymorphic microsatellite loci containing dinucleotide repeats were developed for the freshwater turtle Mauremys rivulata. The number of alleles ranged from five to 18. The observed and expected heterozygosities ranged from 0.19 to 0.79 and 0.46 to 0.90, respectively. These markers may serve as a valuable tool for population genetics analyses and provide information on the evolutionary history of the species.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Six polymorphic microsatellite loci containing dinucleotide repeats were developed for the freshwater turtle Mauremys rivulata. The number of alleles ranged from five to 18. The observed and expected heterozygosities ranged from 0.19 to 0.79 and 0.46 to 0.90, respectively. These markers may serve as a valuable tool for population genetics analyses and provide information on the evolutionary history of the species. |
Xylouri, E; Kotzamanis, Υ P; Athanassopoulou, F; Dong, L; Argyrokastritis, A; Frangiadaki, I Isolation, characterization and sequence determination of Noda virus from sea bass (Dicentrarchus labrax L.) reared in freshwater and marine facilities in Greece Journal Article Bulletin of the Hellenic Veterinary Medical Society, 56 (2), pp. 105–113, 2005. @article{xylouri_isolation_2005, title = {Isolation, characterization and sequence determination of Noda virus from sea bass (Dicentrarchus labrax L.) reared in freshwater and marine facilities in Greece}, author = {E Xylouri and Υ P Kotzamanis and F Athanassopoulou and L Dong and A Argyrokastritis and I Frangiadaki}, year = {2005}, date = {2005-01-01}, journal = {Bulletin of the Hellenic Veterinary Medical Society}, volume = {56}, number = {2}, pages = {105--113}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Mandalakis, M; Apostolaki, M; Stephanou, E G; Stavrakakis, S Mass budget and dynamics of polychlorinated biphenyls in the eastern Mediterranean Sea Journal Article Global Biogeochemical Cycles, 19 (3), pp. 1–16, 2005, ISSN: 08866236. @article{mandalakis_mass_2005, title = {Mass budget and dynamics of polychlorinated biphenyls in the eastern Mediterranean Sea}, author = {M Mandalakis and M Apostolaki and E G Stephanou and S Stavrakakis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-27744457044&doi=10.1029%2f2004GB002403&partnerID=40&md5=c448a5c9bc9bcb79e6dc5fc720676fec}, doi = {10.1029/2004GB002403}, issn = {08866236}, year = {2005}, date = {2005-01-01}, journal = {Global Biogeochemical Cycles}, volume = {19}, number = {3}, pages = {1--16}, abstract = {Polychlorinated biphenyls (PCBs) were measured in dry deposition and sediment trap samples from the eastern Mediterranean and the results of the present and previous studies were synthesized in order to construct a mass balance budget describing the status and dynamics of PCBs in this region. According to our calculations, the burden of total PCBs in the euphotic zone of the eastern Mediterranean should approach 84,000 kg, while the corresponding pool in the overlying atmosphere should be about 162 kg. The atmospheric input of PCBs in the respective water basin through dry and wet deposition should be 300 and 1300 kg yr-1, respectively. On the contrary, air-sea exchange should cause a net volatilization of PCBs from seawater to the atmosphere by 3150 kg yr-1. Moreover, PCBs are removed from the water column with a settling flux of 350 kg yr-1 and under steady state conditions, an additional input of these pollutants should be required to counterbalance the outflows from the water basin. The discharge of urban and industrial sewage, riverine input and transport of more polluted surface waters from western to eastern Mediterranean may account for most of this inflow. In the atmospheric compartment, the removal flux of PCBs due to their destruction by hydroxyl radicals (6650 kg yr-1) was approximately 4 times higher than their deposition flux (wet plus dry). On the basis of these data, 5100 kg of PCBs should enter into the atmosphere of eastern Mediterranean annually in order to achieve steady state conditions. This influx may result from long or short range transport of atmospheric PCBs emitted from contaminated terrestrial surfaces. Copyright 2005 by the American Geophysical Union.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Polychlorinated biphenyls (PCBs) were measured in dry deposition and sediment trap samples from the eastern Mediterranean and the results of the present and previous studies were synthesized in order to construct a mass balance budget describing the status and dynamics of PCBs in this region. According to our calculations, the burden of total PCBs in the euphotic zone of the eastern Mediterranean should approach 84,000 kg, while the corresponding pool in the overlying atmosphere should be about 162 kg. The atmospheric input of PCBs in the respective water basin through dry and wet deposition should be 300 and 1300 kg yr-1, respectively. On the contrary, air-sea exchange should cause a net volatilization of PCBs from seawater to the atmosphere by 3150 kg yr-1. Moreover, PCBs are removed from the water column with a settling flux of 350 kg yr-1 and under steady state conditions, an additional input of these pollutants should be required to counterbalance the outflows from the water basin. The discharge of urban and industrial sewage, riverine input and transport of more polluted surface waters from western to eastern Mediterranean may account for most of this inflow. In the atmospheric compartment, the removal flux of PCBs due to their destruction by hydroxyl radicals (6650 kg yr-1) was approximately 4 times higher than their deposition flux (wet plus dry). On the basis of these data, 5100 kg of PCBs should enter into the atmosphere of eastern Mediterranean annually in order to achieve steady state conditions. This influx may result from long or short range transport of atmospheric PCBs emitted from contaminated terrestrial surfaces. Copyright 2005 by the American Geophysical Union. |
Arvanitidis, C; Atzigeorgiou, G; Koutsoubas, D; Dounas, C; Eleftheriou, A; Koulouri, P Mediterranean lagoons revisited: Weakness and efficiency of the rapid biodiversity assessment techniques in a severely fluctuating environment Journal Article Biodiversity and Conservation, 14 (10), pp. 2347–2359, 2005, ISSN: 09603115. @article{arvanitidis_mediterranean_2005, title = {Mediterranean lagoons revisited: Weakness and efficiency of the rapid biodiversity assessment techniques in a severely fluctuating environment}, author = {C Arvanitidis and G Atzigeorgiou and D Koutsoubas and C Dounas and A Eleftheriou and P Koulouri}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-24944484401&doi=10.1007%2fs10531-004-1668-x&partnerID=40&md5=164959659412afbcf4b012e66162f526}, doi = {10.1007/s10531-004-1668-x}, issn = {09603115}, year = {2005}, date = {2005-01-01}, journal = {Biodiversity and Conservation}, volume = {14}, number = {10}, pages = {2347--2359}, abstract = {The purpose of this paper is to test the performance of rapid biodiversity assessment techniques in the lagoonal environment at the pan-Mediterranean scale. The multivariate techniques can produce patterns of lagoonal biodiversity along the Mediterranean. Additionally, it is shown that the polychaete inventory can preferably be used rather than the aggregation of information at the family level for the purposes of rapid biodiversity assessment. These techniques, however, appear to be weak for the environmental assessment because they cannot detect differences between the naturally disturbed and the anthropogenically impacted lagoons. Both taxonomic distinctness indices are found to be robust in providing meaningful results for rapid biodiversity/environmental assessment when the crustacean inventory and the polychaete and molluscan ones are used for the estimation of the average taxonomic distinctness and of the variation in taxonomic distinctness values, correspondingly. Conversely, information on the distribution of the macrofaunal species to the Mediterranean lagoons appears to be inadequate for the needs of such rapid biodiversity assessment at a regional scale. It is suggested that information on ecological convergence of the macrofaunal species would probably provide biodiversity indices with additional power, at least in the lagoonal environment. © Springer 2005.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The purpose of this paper is to test the performance of rapid biodiversity assessment techniques in the lagoonal environment at the pan-Mediterranean scale. The multivariate techniques can produce patterns of lagoonal biodiversity along the Mediterranean. Additionally, it is shown that the polychaete inventory can preferably be used rather than the aggregation of information at the family level for the purposes of rapid biodiversity assessment. These techniques, however, appear to be weak for the environmental assessment because they cannot detect differences between the naturally disturbed and the anthropogenically impacted lagoons. Both taxonomic distinctness indices are found to be robust in providing meaningful results for rapid biodiversity/environmental assessment when the crustacean inventory and the polychaete and molluscan ones are used for the estimation of the average taxonomic distinctness and of the variation in taxonomic distinctness values, correspondingly. Conversely, information on the distribution of the macrofaunal species to the Mediterranean lagoons appears to be inadequate for the needs of such rapid biodiversity assessment at a regional scale. It is suggested that information on ecological convergence of the macrofaunal species would probably provide biodiversity indices with additional power, at least in the lagoonal environment. © Springer 2005. |
Lika, K; Papandroulakis, N Modeling feeding processes: A test of a new model for sea bream (Sparus aurata) larvae Journal Article Canadian Journal of Fisheries and Aquatic Sciences, 62 , pp. 425 – 435, 2005. @article{lika_modeling_2005, title = {Modeling feeding processes: A test of a new model for sea bream (Sparus aurata) larvae}, author = {K Lika and N Papandroulakis}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-21244489012&partnerID=40&md5=6d09799439dfccab3d0ab5e65d566178}, year = {2005}, date = {2005-01-01}, journal = {Canadian Journal of Fisheries and Aquatic Sciences}, volume = {62}, pages = {425 -- 435}, abstract = {An organism’s feeding rate is governed by constraints imposed by processes associated with consumption. We present a general feeding model that incorporates encounter, successful pursuit, handling, and digestion in one functional representation where we treat digestion as a parallel process. The model produces type II functional response curves. However, the asymptotic maximum feeding rate is determined by the sum of the time spent for handling and digesting a prey minus the gain in time, since the digestion process is parallel to the handling process. We use our model in combination with existing models of encounter, successful pursuit, and digestion to evaluate the feeding rate of fish larvae. We test the model against experimental data for sea bream (Sparus aurata) larvae and find a very close quantitative correspondence between predictions and experiments. Sensitivity analysis shows that for the early developmental stages, the model is sensitive to parameters related to the visual and locomotion abilities of larvae to detect and capture the prey. Later, when they establish these abilities, the choice of accepting or not the prey becomes more important. © 2005 NRC Canada.}, keywords = {}, pubstate = {published}, tppubtype = {article} } An organism’s feeding rate is governed by constraints imposed by processes associated with consumption. We present a general feeding model that incorporates encounter, successful pursuit, handling, and digestion in one functional representation where we treat digestion as a parallel process. The model produces type II functional response curves. However, the asymptotic maximum feeding rate is determined by the sum of the time spent for handling and digesting a prey minus the gain in time, since the digestion process is parallel to the handling process. We use our model in combination with existing models of encounter, successful pursuit, and digestion to evaluate the feeding rate of fish larvae. We test the model against experimental data for sea bream (Sparus aurata) larvae and find a very close quantitative correspondence between predictions and experiments. Sensitivity analysis shows that for the early developmental stages, the model is sensitive to parameters related to the visual and locomotion abilities of larvae to detect and capture the prey. Later, when they establish these abilities, the choice of accepting or not the prey becomes more important. © 2005 NRC Canada. |
Katharios, P; Papadakis, I E; Prapas, A; Dermon, C R; Ampatzis, K; Divanach, P Bulletin of the European Association of Fish Pathologists, 25 (1), pp. 28–31, 2005, ISSN: 01080288. @article{katharios_mortality_2005, title = {Mortality control of viral encephalopathy and retinopathy in 0+ grouper Epinephelus marginatus after prolonged bath in dense Chlorella minutissima culture}, author = {P Katharios and I E Papadakis and A Prapas and C R Dermon and K Ampatzis and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-15744380255&partnerID=40&md5=1882681096115ce168df99dfd0aaf0d8}, issn = {01080288}, year = {2005}, date = {2005-01-01}, journal = {Bulletin of the European Association of Fish Pathologists}, volume = {25}, number = {1}, pages = {28--31}, abstract = {0+ grouper (1±0.2g), presenting symptoms of Viral Encephalopathy and Retinopathy (confirmed by isolation of the virus in SSN-1 cell line and identification by IFAT and DNA sequencing as well as by brain histology), were submitted to seven bath treatment regimes for a 30-day period, using different concentrations of Chlorella minutissima and the antibiotic furaltadone. The cumulative mortalities were 0, 0, 5 and 32% when using 2×108, 108, 5×107 and 2×107 cells of C. minutissima ml-1 respectively, compared to 31-36 % in the control and 55-68% in the antibiotic-treated groups. The beneficial effect, the possible immuno-stimulant and antiviral properties of unicellular micro-algae such as C. minutissima, as well as the role of their concentration in the bath treatment, are discussed.}, keywords = {}, pubstate = {published}, tppubtype = {article} } 0+ grouper (1±0.2g), presenting symptoms of Viral Encephalopathy and Retinopathy (confirmed by isolation of the virus in SSN-1 cell line and identification by IFAT and DNA sequencing as well as by brain histology), were submitted to seven bath treatment regimes for a 30-day period, using different concentrations of Chlorella minutissima and the antibiotic furaltadone. The cumulative mortalities were 0, 0, 5 and 32% when using 2×108, 108, 5×107 and 2×107 cells of C. minutissima ml-1 respectively, compared to 31-36 % in the control and 55-68% in the antibiotic-treated groups. The beneficial effect, the possible immuno-stimulant and antiviral properties of unicellular micro-algae such as C. minutissima, as well as the role of their concentration in the bath treatment, are discussed. |
Gustafsson, Ö; Elmquist, M; Kukulska, Z; Cornelissen, G; Mandalakis, M; Persson, J; Bucheli, T; Reddy, C On the analysis of black carbon in sediments: Evaluation of field data for geochemical consistency Inproceedings Geophysical Research Abstracts, pp. EGU05–A–06089, 2005. @inproceedings{gustafsson_analysis_2005, title = {On the analysis of black carbon in sediments: Evaluation of field data for geochemical consistency}, author = {Ö Gustafsson and M Elmquist and Z Kukulska and G Cornelissen and M Mandalakis and J Persson and T Bucheli and C Reddy}, year = {2005}, date = {2005-01-01}, booktitle = {Geophysical Research Abstracts}, volume = {7}, pages = {EGU05--A--06089}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Koumoundouros, G; Kouttouki, S; Georgakopoulou, E; Papadakis, I; Maingot, E; Kaspiris, P; Kiriakou, Y; Georgiou, G; Divanach, P; Kentouri, M; Mylonas, C C Ontogeny of the shi drum Umbrina cirrosa (Linnaeus 1758), a candidate new species for aquaculture Journal Article Aquaculture Research, 36 (13), pp. 1265–1272, 2005, ISSN: 1355557X. @article{koumoundouros_ontogeny_2005, title = {Ontogeny of the shi drum Umbrina cirrosa (Linnaeus 1758), a candidate new species for aquaculture}, author = {G Koumoundouros and S Kouttouki and E Georgakopoulou and I Papadakis and E Maingot and P Kaspiris and Y Kiriakou and G Georgiou and P Divanach and M Kentouri and C C Mylonas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-25844487755&doi=10.1111%2fj.1365-2109.2005.01314.x&partnerID=40&md5=e26503abd5c1e3f3b24d5805f8231280}, doi = {10.1111/j.1365-2109.2005.01314.x}, issn = {1355557X}, year = {2005}, date = {2005-01-01}, journal = {Aquaculture Research}, volume = {36}, number = {13}, pages = {1265--1272}, abstract = {The ontogeny of shi drum Umbrina cirrosa (Linnaeus 1758), a candidate new species for aquaculture, was studied throughout the entire larval phase. Geometric morphometric analysis revealed two clear inflection points (7.0 and 12.7 mm total length, TL) in the shape ontogeny of this species, separating the studied period into three phases of different allometric priorities. Spline graphs demonstrated that the major non-uniform shape ontogeny correlated with the development of the fins, the anterior dorsal area of the body, the caudal peduncle, the eye and the mouth. Concerning the morphological features, shi drum larvae are characterized by an upward anterior bending of the notochord. The ontogeny of the fins began with the formation of the pectoral buds (2.9 mm TL), continued with the notochord flexion (4.3 mm TL, associated with the caudal fin development), the appearance of the pelvic buds, the first anal rays (4.5 mm TL) and the first dorsal rays (4.8 mm TL). Shi drum juveniles presented 25 vertebrae and the following dominant fin types: D XI,23, AII,6,VI,5, P17 and C17. © 2005 Blackwell Publishing Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The ontogeny of shi drum Umbrina cirrosa (Linnaeus 1758), a candidate new species for aquaculture, was studied throughout the entire larval phase. Geometric morphometric analysis revealed two clear inflection points (7.0 and 12.7 mm total length, TL) in the shape ontogeny of this species, separating the studied period into three phases of different allometric priorities. Spline graphs demonstrated that the major non-uniform shape ontogeny correlated with the development of the fins, the anterior dorsal area of the body, the caudal peduncle, the eye and the mouth. Concerning the morphological features, shi drum larvae are characterized by an upward anterior bending of the notochord. The ontogeny of the fins began with the formation of the pectoral buds (2.9 mm TL), continued with the notochord flexion (4.3 mm TL, associated with the caudal fin development), the appearance of the pelvic buds, the first anal rays (4.5 mm TL) and the first dorsal rays (4.8 mm TL). Shi drum juveniles presented 25 vertebrae and the following dominant fin types: D XI,23, AII,6,VI,5, P17 and C17. © 2005 Blackwell Publishing Ltd. |
Szisch, V; Papandroulakis, N; Fanouraki, E; Pavlidis, M Ontogeny of the thyroid hormones and cortisol in the gilthead sea bream, Sparus aurata Journal Article General and Comparative Endocrinology, 142 , pp. 186 – 192, 2005. @article{szisch_ontogeny_2005, title = {Ontogeny of the thyroid hormones and cortisol in the gilthead sea bream, Sparus aurata}, author = {V Szisch and N Papandroulakis and E Fanouraki and M Pavlidis}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-18144427445&partnerID=40&md5=95ebcdb3f57636df10429eeea10cafa2}, year = {2005}, date = {2005-01-01}, journal = {General and Comparative Endocrinology}, volume = {142}, pages = {186 -- 192}, abstract = {The aim of the study was to elucidate the pattern of changes in the whole body concentrations of thyroid hormones (TH) and cortisol in eggs, pre-larvae, and larvae in the gilthead sea bream, Sparus aurata. The ontogeny of these hormones was related to specific morphological characteristics that characterize early development. TH and cortisol were detected in substantial amounts in the eggs of sea bream. T3 concentration in eggs was found to be higher than that of T4 (T3, 4.02 ± 0.38 ng g-1; T4, 1.63 ± 0.28 ng g-1), as it is often observed in marine teleosts. T4 decreased until hatching (T4, 1.01 ± 0.58 ng g-1), while T3 reached a minimum at the time of first feeding (T3, 0.19 ± 0.02 ng g-1). As development proceeded, both hormones displayed a similar pattern of changes with increasing concentrations until the completion of metamorphosis (T3, 15.84 ± 2.92 ng g-1; T4, 9.78 ± 2.39 ng g-1), indicating that the hypothalamo-adenohypophysial-thyroid axis begins to function soon after first feeding. The pattern of changes of cortisol (F) concentration was almost parallel to that of T4, starting from 0.83 ng g-1 in eggs. Minimum F concentration was observed at hatching (0.39 ± 0.03 ng g-1) and maximum at melanophores and scale formation (14.82 ± 2.71 and 14.82 ± 3.12 ng g-1, respectively). Results provide data for the first time on the ontogeny of thyroid hormones and cortisol during the early development in sea bream and are in agreement with results in other fish species for an important action of TH during early development. © 2005 Elsevier Inc. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The aim of the study was to elucidate the pattern of changes in the whole body concentrations of thyroid hormones (TH) and cortisol in eggs, pre-larvae, and larvae in the gilthead sea bream, Sparus aurata. The ontogeny of these hormones was related to specific morphological characteristics that characterize early development. TH and cortisol were detected in substantial amounts in the eggs of sea bream. T3 concentration in eggs was found to be higher than that of T4 (T3, 4.02 ± 0.38 ng g-1; T4, 1.63 ± 0.28 ng g-1), as it is often observed in marine teleosts. T4 decreased until hatching (T4, 1.01 ± 0.58 ng g-1), while T3 reached a minimum at the time of first feeding (T3, 0.19 ± 0.02 ng g-1). As development proceeded, both hormones displayed a similar pattern of changes with increasing concentrations until the completion of metamorphosis (T3, 15.84 ± 2.92 ng g-1; T4, 9.78 ± 2.39 ng g-1), indicating that the hypothalamo-adenohypophysial-thyroid axis begins to function soon after first feeding. The pattern of changes of cortisol (F) concentration was almost parallel to that of T4, starting from 0.83 ng g-1 in eggs. Minimum F concentration was observed at hatching (0.39 ± 0.03 ng g-1) and maximum at melanophores and scale formation (14.82 ± 2.71 and 14.82 ± 3.12 ng g-1, respectively). Results provide data for the first time on the ontogeny of thyroid hormones and cortisol during the early development in sea bream and are in agreement with results in other fish species for an important action of TH during early development. © 2005 Elsevier Inc. All rights reserved. |
Poulakakis, N; Lymberakis, P; Tsigenopoulos, C S; Magoulas, A; Mylonas, M Phylogenetic relationships and evolutionary history of snake-eyed skink Ablepharus kitaibelii (Sauria: Scincidae) Journal Article Molecular Phylogenetics and Evolution, 34 , pp. 245 – 256, 2005. @article{poulakakis_phylogenetic_2005, title = {Phylogenetic relationships and evolutionary history of snake-eyed skink Ablepharus kitaibelii (Sauria: Scincidae)}, author = {N Poulakakis and P Lymberakis and C S Tsigenopoulos and A Magoulas and M Mylonas}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-11144266691&partnerID=40&md5=9ec5b39f41ecfb4a0f8fdb0d7b315756}, year = {2005}, date = {2005-01-01}, journal = {Molecular Phylogenetics and Evolution}, volume = {34}, pages = {245 -- 256}, abstract = {Sequence data derived from two mitochondrial markers, 16S rRNA and cytochrome b genes, were used to infer the phylogenetic relationships of 38 populations of the snake-eyed skinks of the genus Ablepharus with emphasis on A. kitaibelii from Greece and Turkey. The partition-homogeneity tests indicated that the combined data set was homogeneous, and maximum-parsimony, maximum-likelihood, and Bayesian analyses produced topologically identical trees that revealed a well-resolved phylogeny. All species except A. kitaibelii form monophyletic units. The latter species appears paraphyletic with respect to A. budaki and A. chernovi with populations clustering into two distinct clades. A. chernovi and A. budaki, which have recently been raised to species status, were confirmed as genetically distinct forms. We used sequence divergence and paleogeographic history of the Aegean region to reconstruct a biogeographic evolutionary scenario for A. kitaibelii. © 2004 Elsevier Inc. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Sequence data derived from two mitochondrial markers, 16S rRNA and cytochrome b genes, were used to infer the phylogenetic relationships of 38 populations of the snake-eyed skinks of the genus Ablepharus with emphasis on A. kitaibelii from Greece and Turkey. The partition-homogeneity tests indicated that the combined data set was homogeneous, and maximum-parsimony, maximum-likelihood, and Bayesian analyses produced topologically identical trees that revealed a well-resolved phylogeny. All species except A. kitaibelii form monophyletic units. The latter species appears paraphyletic with respect to A. budaki and A. chernovi with populations clustering into two distinct clades. A. chernovi and A. budaki, which have recently been raised to species status, were confirmed as genetically distinct forms. We used sequence divergence and paleogeographic history of the Aegean region to reconstruct a biogeographic evolutionary scenario for A. kitaibelii. © 2004 Elsevier Inc. All rights reserved. |
Koulouri, P; Dounas, C; Eleftheriou, A Preliminary results on the effect of otter trawling on hyperbenthic communities in Heraklion Bay (Eastern Mediterranean, Cretan Sea) Journal Article American Fisheries Society Symposium, 41 , pp. 529–537, 2005. @article{koulouri_preliminary_2005, title = {Preliminary results on the effect of otter trawling on hyperbenthic communities in Heraklion Bay (Eastern Mediterranean, Cretan Sea)}, author = {P Koulouri and C Dounas and A Eleftheriou}, year = {2005}, date = {2005-01-01}, journal = {American Fisheries Society Symposium}, volume = {41}, pages = {529--537}, abstract = {Although the benthopelagic fish species are a focus of commercial exploitation, relatively little attention has been paid to the small-sized invertebrates (0.5-20mm) living on or very close to the seabed, thus inhabiting the same biotope, known as well as “hyperbenthos”. Recently interest in this faunal group has increased, as many demersal fish and epibenthic crustaceans have been found to feed on hyperbenthic animals, for at least part of their lives. Otter trawls, the most common gear used for demersal fishing, result in significant disturbance of the sediment-water interface. Animals that are disturbed by the passage of a trawl may become more available to predators and scavengers. We have been unable to find any reports of studies of the impacts of towed fishing gears, including otter trawls, on hyperbenthos. We studied these effects on the Mediterranean continental shelf in Heraklion Bay (Cretan Sea), using a novel apparatus to simulate the contact of otter trawl groundrope with the sea bed. A modified three-level hyperbenthic sledge was used for collecting disturbed (groundrope present) and undisturbed (without groundrope) macrofaunal samples at a towing speed typical of the local commercial vessels. Observations were made in an area being actively fished, before and during the trawling season. The preliminary results reported here indicate that trawling causes significant changes in the structure of hyperbenthic communities.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Although the benthopelagic fish species are a focus of commercial exploitation, relatively little attention has been paid to the small-sized invertebrates (0.5-20mm) living on or very close to the seabed, thus inhabiting the same biotope, known as well as “hyperbenthos”. Recently interest in this faunal group has increased, as many demersal fish and epibenthic crustaceans have been found to feed on hyperbenthic animals, for at least part of their lives. Otter trawls, the most common gear used for demersal fishing, result in significant disturbance of the sediment-water interface. Animals that are disturbed by the passage of a trawl may become more available to predators and scavengers. We have been unable to find any reports of studies of the impacts of towed fishing gears, including otter trawls, on hyperbenthos. We studied these effects on the Mediterranean continental shelf in Heraklion Bay (Cretan Sea), using a novel apparatus to simulate the contact of otter trawl groundrope with the sea bed. A modified three-level hyperbenthic sledge was used for collecting disturbed (groundrope present) and undisturbed (without groundrope) macrofaunal samples at a towing speed typical of the local commercial vessels. Observations were made in an area being actively fished, before and during the trawling season. The preliminary results reported here indicate that trawling causes significant changes in the structure of hyperbenthic communities. |
Athanassopoulou, F; Ragias, V; Vagianou, St.; Cave, Di D; Rigos, G; Papathanasiou, G; Georgoulakis, J Bulletin of the European Association of Fish Pathologists, 25 (6), pp. 256–261, 2005, ISSN: 01080288. @article{athanassopoulou_report_2005, title = {Report of Sparicotyle (Microcotyle) chrysophrii Van Beneden and Hesse 1863, Atrispinum seminalis Euzet and Maillard 1973 and Polylabris tubicirrus Paperna and Kohn 1964 (Monogenea) on captive sea bream (Sparus aurata) and sharp snout sea bream (Diplodus puntazzo) in coastal Greece and Italy}, author = {F Athanassopoulou and V Ragias and St. Vagianou and D Di Cave and G Rigos and G Papathanasiou and J Georgoulakis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-32644449166&partnerID=40&md5=2a4af286e79a6d976aab89a00362db06}, issn = {01080288}, year = {2005}, date = {2005-01-01}, journal = {Bulletin of the European Association of Fish Pathologists}, volume = {25}, number = {6}, pages = {256--261}, abstract = {During the years 2000-2003, caged gilthead sea bream (Sparus aurata L.) and sharp snout sea bream (Diplodus puntazzo C.) from two farms in Greece and two farms in Italy (the Central Thyrrenian Sea) were examined. Sparus aurata from Greece was moderately infected with Furnestinia echeneis and Sparicotyle chrysophrii. Diplodus puntazzo from the Greek farms were infected with three polyopisthocotylean monogeneans: Atrispinum salpae, A. seminalis and S. chrysophrii. In Italy, on the gills of cultivated S. aurata, a polyopisthocotylean parasite was identified as Polylabris tubicirrus which is considered host specific and only affecting breams of the genus Diplodus. Furthermore, D. puntazzo and S. aurata showed the transfer of the monogenean S. chrysophrii from S. aurata (its type host) to D. puntazzo which is never found infected in wild conditions by this parasite. The presence of these parasites, and in particular S. chrysophrii in cultured D. puntazzo, is an important finding for aquaculturists in terms of potential disease problems.}, keywords = {}, pubstate = {published}, tppubtype = {article} } During the years 2000-2003, caged gilthead sea bream (Sparus aurata L.) and sharp snout sea bream (Diplodus puntazzo C.) from two farms in Greece and two farms in Italy (the Central Thyrrenian Sea) were examined. Sparus aurata from Greece was moderately infected with Furnestinia echeneis and Sparicotyle chrysophrii. Diplodus puntazzo from the Greek farms were infected with three polyopisthocotylean monogeneans: Atrispinum salpae, A. seminalis and S. chrysophrii. In Italy, on the gills of cultivated S. aurata, a polyopisthocotylean parasite was identified as Polylabris tubicirrus which is considered host specific and only affecting breams of the genus Diplodus. Furthermore, D. puntazzo and S. aurata showed the transfer of the monogenean S. chrysophrii from S. aurata (its type host) to D. puntazzo which is never found infected in wild conditions by this parasite. The presence of these parasites, and in particular S. chrysophrii in cultured D. puntazzo, is an important finding for aquaculturists in terms of potential disease problems. |
Polymenakou, P N; Tselepides, A; Stephanou, E G Continental Shelf Research, 25 , pp. 2196–2212, 2005. @article{polymenakou_study_2005, title = {Study of the mineralization effect on the distribution of lipids in sediments from the Cretan Sea: Evidence for hydrocarbon degradation and starvation stress}, author = {P N Polymenakou and A Tselepides and E G Stephanou}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-27744476221&partnerID=40&md5=d20fdba5930d83604c3f1010a58783da}, year = {2005}, date = {2005-01-01}, journal = {Continental Shelf Research}, volume = {25}, pages = {2196--2212}, abstract = {Sedimentary diagenetic processes alter the composition and distribution of different lipid compounds. In the present study alterations mediated by microbial communities were investigated along a bathymetric gradient (100 m at 35°23'N-25°09'E, 617 m at 35°33'N- 25°08'E, 1494 m at 35°44'N-25°08'E) over the continental margin of northern Crete (Greece, Eastern Mediterranean Sea). Bacterial abundances and distribution were studied using phospholipid linked fatty acids (PLFA), in the range of C8-C22, released from intact phospholipids. Lipid components (aliphatic hydrocarbons, free fatty acids, glycerides and glycolipids) were studied over a 2-month incubation period. Carbon mineralization rates at all stations indicated an uneven distribution of active aerobic bacteria with values decreasing towards the deeper stations. PLFA homologue profiles denoted that aerobic gram negative and sulfur oxidizing bacteria dominated microbial communities while the anaerobic, gram positive and sulfate reducing bacteria occurred only in traces. The n-alkane (NA) composition revealed a strong predominance of homologues with odd carbon numbers suggesting an important terrestrial contribution to the sediments. The estimated descriptive ratios of NA, the sum of short chain NA (C15-C20) and long chain NA (C21-C 36) to 17α(H),21β(H)-C30-hopane, before and after a two-month incubation period, indicated the occurrence of hydrocarbon degradation processes. Increased ratios of saturated to unsaturated fatty acids were also recorded after the incubation indicating the starvation of bacterial communities by the end of the experiments. © 2005 Elsevier Ltd. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Sedimentary diagenetic processes alter the composition and distribution of different lipid compounds. In the present study alterations mediated by microbial communities were investigated along a bathymetric gradient (100 m at 35°23'N-25°09'E, 617 m at 35°33'N- 25°08'E, 1494 m at 35°44'N-25°08'E) over the continental margin of northern Crete (Greece, Eastern Mediterranean Sea). Bacterial abundances and distribution were studied using phospholipid linked fatty acids (PLFA), in the range of C8-C22, released from intact phospholipids. Lipid components (aliphatic hydrocarbons, free fatty acids, glycerides and glycolipids) were studied over a 2-month incubation period. Carbon mineralization rates at all stations indicated an uneven distribution of active aerobic bacteria with values decreasing towards the deeper stations. PLFA homologue profiles denoted that aerobic gram negative and sulfur oxidizing bacteria dominated microbial communities while the anaerobic, gram positive and sulfate reducing bacteria occurred only in traces. The n-alkane (NA) composition revealed a strong predominance of homologues with odd carbon numbers suggesting an important terrestrial contribution to the sediments. The estimated descriptive ratios of NA, the sum of short chain NA (C15-C20) and long chain NA (C21-C 36) to 17α(H),21β(H)-C30-hopane, before and after a two-month incubation period, indicated the occurrence of hydrocarbon degradation processes. Increased ratios of saturated to unsaturated fatty acids were also recorded after the incubation indicating the starvation of bacterial communities by the end of the experiments. © 2005 Elsevier Ltd. All rights reserved. |
Manoudis, G; Antoniadou, Chryssanthi; Dounas, C; Chintiroglou, Chariton Charles Successional stages of experimental artificial reefs deployed in Vistonikos gulf (North SAegean Sea, Greece): Preliminary results Journal Article Belgian Journal of Zoology, 135 , pp. 209–215, 2005. @article{manoudis_successional_2005, title = {Successional stages of experimental artificial reefs deployed in Vistonikos gulf (North SAegean Sea, Greece): Preliminary results}, author = {G Manoudis and Chryssanthi Antoniadou and C Dounas and Chariton Charles Chintiroglou}, year = {2005}, date = {2005-01-01}, journal = {Belgian Journal of Zoology}, volume = {135}, pages = {209--215}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Koutandos, E; Prinos, P Supercritical free-surface turbulent flow over a semicircular obstacle Inproceedings pp. 1366–1376, 2005, (Publication Title: XXXI IAHR Congress). @inproceedings{koutandos_supercritical_2005, title = {Supercritical free-surface turbulent flow over a semicircular obstacle}, author = {E Koutandos and P Prinos}, year = {2005}, date = {2005-01-01}, volume = {1}, pages = {1366--1376}, note = {Publication Title: XXXI IAHR Congress}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Chatzifotis, S; Pavlidis, M; Jimeno, C D; Vardanis, G; Sterioti, A; Divanach, P The effect of different carotenoid sources on skin coloration of cultured red porgy (Pagrus pagrus) Journal Article Aquaculture Research, 36 (15), pp. 1517–1525, 2005, ISSN: 1355557X. @article{chatzifotis_effect_2005-1, title = {The effect of different carotenoid sources on skin coloration of cultured red porgy (Pagrus pagrus)}, author = {S Chatzifotis and M Pavlidis and C D Jimeno and G Vardanis and A Sterioti and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-28144454613&doi=10.1111%2fj.1365-2109.2005.01374.x&partnerID=40&md5=70151f9b5623aeec3809a55ae9d2a95d}, doi = {10.1111/j.1365-2109.2005.01374.x}, issn = {1355557X}, year = {2005}, date = {2005-01-01}, journal = {Aquaculture Research}, volume = {36}, number = {15}, pages = {1517--1525}, abstract = {This study presents data on the effect of carotenoid sources on skin coloration of red porgy (Pagrus pagrus). Three experiments were conducted: in the first, fish were fed an astaxanthin (Naturose®)-supplemented diet, while the second fish received diets supplemented with β-carotene (Rovimix β-caroten®) or lycopene (Lyc-O-Mato®): Carotenoids were added to the level of 100 ppm in each diet, while a non-carotenoid-supplemented diet served as a control. In the third experiment, the effect of dietary protein/carbohydrate ratio on melanin content in the skin was investigated. For this experimentation, four diets were formulated to contain 50/23, 40/32, 30/48 and 20/59 protein/carbohydrate ratio. Naturose astaxanthin increased total carotenoid content in the dorsal skin area while β-carotene and lycopene seem to have had no significant effect. Naturose was the only carotenoid source that had a significant effect on skin hue, promoting a reddish coloration to the dorsal skin area and a ventral hue similar to wild red porgy. No apparent effect of carotenoid source on skin melanin content was observed. In contrast, dietary protein/ carbohydrate ratio affected melanin content in the skin. The fish fed the 50/23 diet showed significantly higher values. Farmed red porgy had eight times higher dorsal-skin melanin content than wild ones. © 2005 Blackwell Publishing Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study presents data on the effect of carotenoid sources on skin coloration of red porgy (Pagrus pagrus). Three experiments were conducted: in the first, fish were fed an astaxanthin (Naturose®)-supplemented diet, while the second fish received diets supplemented with β-carotene (Rovimix β-caroten®) or lycopene (Lyc-O-Mato®): Carotenoids were added to the level of 100 ppm in each diet, while a non-carotenoid-supplemented diet served as a control. In the third experiment, the effect of dietary protein/carbohydrate ratio on melanin content in the skin was investigated. For this experimentation, four diets were formulated to contain 50/23, 40/32, 30/48 and 20/59 protein/carbohydrate ratio. Naturose astaxanthin increased total carotenoid content in the dorsal skin area while β-carotene and lycopene seem to have had no significant effect. Naturose was the only carotenoid source that had a significant effect on skin hue, promoting a reddish coloration to the dorsal skin area and a ventral hue similar to wild red porgy. No apparent effect of carotenoid source on skin melanin content was observed. In contrast, dietary protein/ carbohydrate ratio affected melanin content in the skin. The fish fed the 50/23 diet showed significantly higher values. Farmed red porgy had eight times higher dorsal-skin melanin content than wild ones. © 2005 Blackwell Publishing Ltd. |
Dounas, C; Davies, I; Hayes, P; Arvanitidis, C; Koulouri, P The effect of different types of otter trawl groundrope on benthic nutrient fluxes and sediment biogeochemistry Journal Article American Fisheries Society Symposium, 41 , pp. 539–544, 2005. @article{dounas_effect_2005, title = {The effect of different types of otter trawl groundrope on benthic nutrient fluxes and sediment biogeochemistry}, author = {C Dounas and I Davies and P Hayes and C Arvanitidis and P Koulouri}, url = {http://fisheries.org/shop/x54041xm}, year = {2005}, date = {2005-01-01}, journal = {American Fisheries Society Symposium}, volume = {41}, pages = {539--544}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Kasapidis, P; Magoulas, A; Mylonas, M; Zouros, E The phylogeography of the gecko Cyrtopodion kotschyi (Reptilia: Gekkonidae) in the Aegean archipelago Journal Article Molecular Phylogenetics and Evolution, 35 (3), pp. 612–623, 2005, ISSN: 10557903. @article{kasapidis_phylogeography_2005, title = {The phylogeography of the gecko Cyrtopodion kotschyi (Reptilia: Gekkonidae) in the Aegean archipelago}, author = {P Kasapidis and A Magoulas and M Mylonas and E Zouros}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-18144425199&doi=10.1016%2fj.ympev.2005.02.005&partnerID=40&md5=51a9fa8e77d60205ccf8124439085449}, doi = {10.1016/j.ympev.2005.02.005}, issn = {10557903}, year = {2005}, date = {2005-01-01}, journal = {Molecular Phylogenetics and Evolution}, volume = {35}, number = {3}, pages = {612--623}, abstract = {Cyrtopodion kotschyi is a small gecko, widely distributed on the islands of the Aegean archipelago and the adjacent mainland. We unveiled the phylogeography of the species by using a portion of the cytochrome oxidase I mitochondrial DNA gene from 35 insular and mainland populations. The distinct geographic distribution of the major clades of the phylogenetic tree and its topology suggest a spatial and temporal sequence of phylogenetic separations that coincide with some major paleogeographical separations during the geological history of the Aegean and support a mainly vicariant pattern of differentiation. The separation times and 95% confidence limits among the different clades were estimated according to two different paleogeographical scenarios. The very high interpopulation genetic divergence (up to 20% uncorrected pairwise distances) and the better concordance between paleogeographical and phylogenetic separations for one of the scenarios suggest that species differentiation started during Miocene (about 10 Mya) due to the fragmentation of the united landmass of "Ägäis" that was Aegean at that time. © 2005 Elsevier Inc. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Cyrtopodion kotschyi is a small gecko, widely distributed on the islands of the Aegean archipelago and the adjacent mainland. We unveiled the phylogeography of the species by using a portion of the cytochrome oxidase I mitochondrial DNA gene from 35 insular and mainland populations. The distinct geographic distribution of the major clades of the phylogenetic tree and its topology suggest a spatial and temporal sequence of phylogenetic separations that coincide with some major paleogeographical separations during the geological history of the Aegean and support a mainly vicariant pattern of differentiation. The separation times and 95% confidence limits among the different clades were estimated according to two different paleogeographical scenarios. The very high interpopulation genetic divergence (up to 20% uncorrected pairwise distances) and the better concordance between paleogeographical and phylogenetic separations for one of the scenarios suggest that species differentiation started during Miocene (about 10 Mya) due to the fragmentation of the united landmass of "Ägäis" that was Aegean at that time. © 2005 Elsevier Inc. All rights reserved. |
Kasapidis, P; Suchentrunk, F; Magoulas, A; Kotoulas, G Molecular Phylogenetics and Evolution, 34 (1), pp. 55–66, 2005, ISSN: 10557903. @article{kasapidis_shaping_2005, title = {The shaping of mitochondrial DNA phylogeographic patterns of the brown hare (Lepus europaeus) under the combined influence of Late Pleistocene climatic fluctuations and anthropogenic translocations}, author = {P Kasapidis and F Suchentrunk and A Magoulas and G Kotoulas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-9744258860&doi=10.1016%2fj.ympev.2004.09.007&partnerID=40&md5=a65c976ffbb7419b3edd624a3efbeb4d}, doi = {10.1016/j.ympev.2004.09.007}, issn = {10557903}, year = {2005}, date = {2005-01-01}, journal = {Molecular Phylogenetics and Evolution}, volume = {34}, number = {1}, pages = {55--66}, abstract = {The phylogeographic structure of the brown hare (Lepus europaeus) was studied by analysing mtDNA control region sequences of 98 individuals from continental and insular Greece, Bulgaria, Cyprus and northern Israel, together with 44 published sequences from Italy and central Europe. We found two distinct clades separated by an average nucleotide divergence of 6.6%, which may correspond to a Balkan and to an Asia Minor refugium. The estimated time of separation of the two clades was dated back to 105,000- 490,000 years ago. These two clades coexist in the area of northeastern Greece and Bulgaria, most likely as a result of a post-glacial northward expansion. Within the southern Balkan refugium, network analyses showed geographical structuring, which supports the hypothesis of several isolated Late Pleistocene populations. The central European and Italian populations appear to have originated from a non-detected northern Balkan population that was genetically closely related to some northern Greek populations, as a result of postglacial expansion, translocations or a combination of both. Moreover, several cases of ancient and recent translocations by humans were detected, especially for some island populations, while the eastern Aegean islands off the Asia Minor coast were most likely colonized naturally through Late Pleistocene land bridge connection. The genetic analysis presented here provides a framework for designing proper conservation and management guidelines for this species. © 2004 Elsevier Inc. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The phylogeographic structure of the brown hare (Lepus europaeus) was studied by analysing mtDNA control region sequences of 98 individuals from continental and insular Greece, Bulgaria, Cyprus and northern Israel, together with 44 published sequences from Italy and central Europe. We found two distinct clades separated by an average nucleotide divergence of 6.6%, which may correspond to a Balkan and to an Asia Minor refugium. The estimated time of separation of the two clades was dated back to 105,000- 490,000 years ago. These two clades coexist in the area of northeastern Greece and Bulgaria, most likely as a result of a post-glacial northward expansion. Within the southern Balkan refugium, network analyses showed geographical structuring, which supports the hypothesis of several isolated Late Pleistocene populations. The central European and Italian populations appear to have originated from a non-detected northern Balkan population that was genetically closely related to some northern Greek populations, as a result of postglacial expansion, translocations or a combination of both. Moreover, several cases of ancient and recent translocations by humans were detected, especially for some island populations, while the eastern Aegean islands off the Asia Minor coast were most likely colonized naturally through Late Pleistocene land bridge connection. The genetic analysis presented here provides a framework for designing proper conservation and management guidelines for this species. © 2004 Elsevier Inc. All rights reserved. |
Katharios, P; Smullen, R P; Inglis, V The use of the polychaete worm Nereis virens eggs as vehicle for the delivery of oxytetracycline in Solea solea larvae Journal Article Aquaculture, 243 (1-4), pp. 1–7, 2005, ISSN: 00448486. @article{katharios_use_2005, title = {The use of the polychaete worm Nereis virens eggs as vehicle for the delivery of oxytetracycline in Solea solea larvae}, author = {P Katharios and R P Smullen and V Inglis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-10644230309&doi=10.1016%2fj.aquaculture.2004.09.031&partnerID=40&md5=cd90fc4dea25c5202c986ac6b4710655}, doi = {10.1016/j.aquaculture.2004.09.031}, issn = {00448486}, year = {2005}, date = {2005-01-01}, journal = {Aquaculture}, volume = {243}, number = {1-4}, pages = {1--7}, abstract = {The eggs of the polychaete worm Nereis virens were used as a vehicle for the delivery of oxytetracycline (OTC) in Dover sole, Solea solea larvae. The eggs were osmotically loaded, and the level of antibiotic was estimated by HPLC and a microbiological bioassay. The amount of OTC in the N. virens eggs was over 1 μg per egg, which was considered more than sufficient for a therapeutic dose for larval fish. The loaded eggs were tested on the sole larvae, and the results of the experiment (9 μg OTC per fish larva) indicated that the eggs of the Nereis worm can be used as biocarriers of water soluble drugs. © 2004 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The eggs of the polychaete worm Nereis virens were used as a vehicle for the delivery of oxytetracycline (OTC) in Dover sole, Solea solea larvae. The eggs were osmotically loaded, and the level of antibiotic was estimated by HPLC and a microbiological bioassay. The amount of OTC in the N. virens eggs was over 1 μg per egg, which was considered more than sufficient for a therapeutic dose for larval fish. The loaded eggs were tested on the sole larvae, and the results of the experiment (9 μg OTC per fish larva) indicated that the eggs of the Nereis worm can be used as biocarriers of water soluble drugs. © 2004 Elsevier B.V. All rights reserved. |
2004 |
Grigorakis, Kriton; Alexis, Maria; Gialamas, Ioannis; Nikolopoulou, Demetra Sensory, microbiological, and chemical spoilage of cultured common sea bass ( Dicentrarchus labrax) stored in ice: a seasonal differentiation Journal Article European Food Research and Technology, 219 (6), pp. 584–587, 2004, ISSN: 1438-2377, 1438-2385. @article{grigorakis_sensory_2004, title = {Sensory, microbiological, and chemical spoilage of cultured common sea bass ( Dicentrarchus labrax) stored in ice: a seasonal differentiation}, author = {Kriton Grigorakis and Maria Alexis and Ioannis Gialamas and Demetra Nikolopoulou}, url = {http://link.springer.com/10.1007/s00217-004-1005-3}, doi = {10.1007/s00217-004-1005-3}, issn = {1438-2377, 1438-2385}, year = {2004}, date = {2004-11-01}, urldate = {2020-08-10}, journal = {European Food Research and Technology}, volume = {219}, number = {6}, pages = {584--587}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Marcouli, P A; Alexis, M N; Andriopoulou, A; Iliopoulou-Georgudaki, J Development of a reference diet for use in indispensable amino acid requirement studies of gilthead seabream Sparus aurata L. Journal Article Aquaculture Nutrition, 10 (5), pp. 335–343, 2004, ISSN: 1353-5773, 1365-2095. @article{marcouli_development_2004, title = {Development of a reference diet for use in indispensable amino acid requirement studies of gilthead seabream Sparus aurata L.}, author = {P A Marcouli and M N Alexis and A Andriopoulou and J Iliopoulou-Georgudaki}, url = {http://doi.wiley.com/10.1111/j.1365-2095.2004.00308.x}, doi = {10.1111/j.1365-2095.2004.00308.x}, issn = {1353-5773, 1365-2095}, year = {2004}, date = {2004-10-01}, urldate = {2020-08-17}, journal = {Aquaculture Nutrition}, volume = {10}, number = {5}, pages = {335--343}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
P., Katharios; I.E., Papadakis; A., Prapas; C.R., Dermon; K., Ampatzis; Divanach, P Mortality control of VNN disease in 0+ grouper, Epinephelus marginatus after prolonged bath in dense Chlorella minutissima culture. Inproceedings EAS, (Ed.): European Aquaculture Society, Biotechnologies for Quality, Barcelona, Spain, 2004. @inproceedings{katharios_p_mortality_2004, title = {Mortality control of VNN disease in 0+ grouper, Epinephelus marginatus after prolonged bath in dense Chlorella minutissima culture.}, author = {Katharios P. and Papadakis I.E. and Prapas A. and Dermon C.R. and Ampatzis K. and P Divanach}, editor = {EAS}, year = {2004}, date = {2004-10-01}, booktitle = {European Aquaculture Society, Biotechnologies for Quality}, address = {Barcelona, Spain}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Koumoundouros, G; Carrillo, J; Divanach, P; Kentouri, M The rearing of common dentex Dentex dentex (L.) During the hatchery and on-growing phases Journal Article Aquaculture, 240 (1-4), pp. 165–173, 2004, ISSN: 00448486. @article{koumoundouros_rearing_2004, title = {The rearing of common dentex Dentex dentex (L.) During the hatchery and on-growing phases}, author = {G Koumoundouros and J Carrillo and P Divanach and M Kentouri}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848604000869}, doi = {10.1016/j.aquaculture.2004.01.038}, issn = {00448486}, year = {2004}, date = {2004-10-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {240}, number = {1-4}, pages = {165--173}, abstract = {In the current paper we present the rearing methodology of common dentex (Dentex dentex), a promising candidate for Mediterranean marine aquaculture, from the larval to the on-growing phase. The results are discussed in terms of the efficiency of rearing methodology followed, for the mass production of the species. © 2004 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In the current paper we present the rearing methodology of common dentex (Dentex dentex), a promising candidate for Mediterranean marine aquaculture, from the larval to the on-growing phase. The results are discussed in terms of the efficiency of rearing methodology followed, for the mass production of the species. © 2004 Elsevier B.V. All rights reserved. |
Koutandos, E V; Karambas, Th. V; Koutitas, C G Floating Breakwater Response to Waves Action Using a Boussinesq Model Coupled with a 2DV Elliptic Solver Journal Article Journal of Waterway, Port, Coastal, and Ocean Engineering, 130 (5), pp. 243–255, 2004, ISSN: 0733-950X, 1943-5460. @article{koutandos_floating_2004, title = {Floating Breakwater Response to Waves Action Using a Boussinesq Model Coupled with a 2DV Elliptic Solver}, author = {E V Koutandos and Th. V Karambas and C G Koutitas}, url = {http://ascelibrary.org/doi/10.1061/%28ASCE%290733-950X%282004%29130%3A5%28243%29}, doi = {10.1061/(ASCE)0733-950X(2004)130:5(243)}, issn = {0733-950X, 1943-5460}, year = {2004}, date = {2004-09-01}, urldate = {2020-08-18}, journal = {Journal of Waterway, Port, Coastal, and Ocean Engineering}, volume = {130}, number = {5}, pages = {243--255}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Kasapidis, P; Planes, S; Laurent, V; Quinta, R; Teia, A; Lenfant, R; Darivianakis, S; Terzoglou, V; Kotoulas, G; Magoulas, A Stock discrimination and temporal and spatial genetic variation of sardine (Sardina pilchardus) in northeastern Atlantic, with a combined analysis of nuclear (microsatellites and allozymes) and mitochondrial DNA markers. Inproceedings Kasapidis, P (Ed.): 2004 ICES Annual Science Conference, 2004. @inproceedings{kasapidis_stock_2004, title = {Stock discrimination and temporal and spatial genetic variation of sardine (Sardina pilchardus) in northeastern Atlantic, with a combined analysis of nuclear (microsatellites and allozymes) and mitochondrial DNA markers.}, author = {P Kasapidis and S Planes and V Laurent and R Quinta and A Teia and R Lenfant and S Darivianakis and V Terzoglou and G Kotoulas and A Magoulas}, editor = {P Kasapidis}, year = {2004}, date = {2004-09-01}, booktitle = {2004 ICES Annual Science Conference}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Sfakianakis, D G; Koumoundouros, G; Divanach, P; Kentouri, M Aquaculture, 232 (1-4), pp. 407–424, 2004, ISSN: 00448486. @article{sfakianakis_osteological_2004, title = {Osteological development of the vertebral column and of the fins in Pagellus erythrinus (L. 1758). Temperature effect on the developmental plasticity and morpho-anatomical abnormalities}, author = {D G Sfakianakis and G Koumoundouros and P Divanach and M Kentouri}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848603005623}, doi = {10.1016/j.aquaculture.2003.08.014}, issn = {00448486}, year = {2004}, date = {2004-04-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {232}, number = {1-4}, pages = {407--424}, abstract = {The osteological ontogeny of Pagellus erythrinus (Linnaeus, 1758) and its temperature-induced plasticity is studied. The ontogeny of the skeleton follows the pattern described in all other Sparidae studied so far: the onset of the ontogeny of the vertebral column and the caudal fin is at 4.3 mm total length (TL) and the completion at 17.6 and 15.4 mm TL, respectively. Dorsal and anal fin development appears at about 5.2 mm TL and completes at 7.5 mm TL. The pelvic are the last fins to develop (7.5-11.5 mm TL). Although the pectorals are the only fins present before first feeding, their ontogeny is completed only after 12.0 mm TL. Temperature affects the developmental rate of the skeleton giving as much as 10.9% of differentiation in total length between 16 and 21°C (caudal lepidotrichia), 9.2% between 16 and 18°C (caudal lepidotrichia) and 6.7% between 18 and 21°C (pelvic lepidotrichia). The role of temperature in developmental plasticity is enhanced by the induction of abnormalities mainly in the area of the caudal fin, which reached (in total) 75% occurrence in the higher temperature regime (23°C) tested. The results of the skeletal ontogeny are compared with the pattern of development of the Sparidae, and are discussed in respect to the role of temperature in the developmental plasticity of fish. © 2004 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The osteological ontogeny of Pagellus erythrinus (Linnaeus, 1758) and its temperature-induced plasticity is studied. The ontogeny of the skeleton follows the pattern described in all other Sparidae studied so far: the onset of the ontogeny of the vertebral column and the caudal fin is at 4.3 mm total length (TL) and the completion at 17.6 and 15.4 mm TL, respectively. Dorsal and anal fin development appears at about 5.2 mm TL and completes at 7.5 mm TL. The pelvic are the last fins to develop (7.5-11.5 mm TL). Although the pectorals are the only fins present before first feeding, their ontogeny is completed only after 12.0 mm TL. Temperature affects the developmental rate of the skeleton giving as much as 10.9% of differentiation in total length between 16 and 21°C (caudal lepidotrichia), 9.2% between 16 and 18°C (caudal lepidotrichia) and 6.7% between 18 and 21°C (pelvic lepidotrichia). The role of temperature in developmental plasticity is enhanced by the induction of abnormalities mainly in the area of the caudal fin, which reached (in total) 75% occurrence in the higher temperature regime (23°C) tested. The results of the skeletal ontogeny are compared with the pattern of development of the Sparidae, and are discussed in respect to the role of temperature in the developmental plasticity of fish. © 2004 Elsevier B.V. All rights reserved. |
Alarcón, J A; Magoulas, A; Georgakopoulos, T; Zouros, E; Alvarez, M C Genetic comparison of wild and cultivated European populations of the gilthead sea bream (Sparus aurata) Journal Article Aquaculture, 230 (1-4), pp. 65–80, 2004, ISSN: 00448486. @article{alarcon_genetic_2004, title = {Genetic comparison of wild and cultivated European populations of the gilthead sea bream (Sparus aurata)}, author = {J A Alarcón and A Magoulas and T Georgakopoulos and E Zouros and M C Alvarez}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848603004344}, doi = {10.1016/S0044-8486(03)00434-4}, issn = {00448486}, year = {2004}, date = {2004-02-01}, urldate = {2020-08-09}, journal = {Aquaculture}, volume = {230}, number = {1-4}, pages = {65--80}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Katharios, P; Pavlidis, M; Iliopoulou-Georgudaki, J Accumulation of ivermectin in the brain of sea bream, Sparus aurata after intraperitoneal administration Journal Article Environmental Toxicology and Pharmacology, 17 (1), pp. 9–12, 2004, ISSN: 13826689. @article{katharios_accumulation_2004, title = {Accumulation of ivermectin in the brain of sea bream, Sparus aurata after intraperitoneal administration}, author = {P Katharios and M Pavlidis and J Iliopoulou-Georgudaki}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-2342451297&doi=10.1016%2fj.etap.2004.01.003&partnerID=40&md5=a461dd2f65ec8b5bfa8e6a22e6b2677d}, doi = {10.1016/j.etap.2004.01.003}, issn = {13826689}, year = {2004}, date = {2004-01-01}, journal = {Environmental Toxicology and Pharmacology}, volume = {17}, number = {1}, pages = {9--12}, abstract = {Ivermectin, which is widely used in veterinary and human, has been considered safe due to its inability to penetrate into the central nervous system of higher vertebrates. This paper presents data on the ability of the drug to cross the blood-brain barrier of the marine teleost sea bream, Sparus aurata and accumulate in the brain. The concentration of the drug in the brain and the serum of the fish was assessed by the use of a direct competitive ELISA commercial kit. Our results showed a rapid uptake of the substance by the brain of the fish reaching a maximum concentration of 98.9 ng g-1 8 h post treatment. The trend of the absorption of the drug in brain followed that of the blood. Concentration of the drug in the brain remained high at each sampling point over the 24 h duration of the experiment. In view of these findings, the need of study of the role of the blood-brain barrier and particularly the multidrug resistance mechanism in sea bream is outlined. © 2004 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Ivermectin, which is widely used in veterinary and human, has been considered safe due to its inability to penetrate into the central nervous system of higher vertebrates. This paper presents data on the ability of the drug to cross the blood-brain barrier of the marine teleost sea bream, Sparus aurata and accumulate in the brain. The concentration of the drug in the brain and the serum of the fish was assessed by the use of a direct competitive ELISA commercial kit. Our results showed a rapid uptake of the substance by the brain of the fish reaching a maximum concentration of 98.9 ng g-1 8 h post treatment. The trend of the absorption of the drug in brain followed that of the blood. Concentration of the drug in the brain remained high at each sampling point over the 24 h duration of the experiment. In view of these findings, the need of study of the role of the blood-brain barrier and particularly the multidrug resistance mechanism in sea bream is outlined. © 2004 Elsevier B.V. All rights reserved. |
Galay-Burgos, M; Llewellyn, L; Mylonas, C C; Canario, A V M; Zanuy, S; Sweeney, G E Analysis of the Sox gene family in the European sea bass (Dicentrarchus labrax) Journal Article Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 137 (2), pp. 279–284, 2004, ISSN: 10964959, (Publisher: Elsevier Inc.). @article{galay-burgos_analysis_2004, title = {Analysis of the Sox gene family in the European sea bass (Dicentrarchus labrax)}, author = {M Galay-Burgos and L Llewellyn and C C Mylonas and A V M Canario and S Zanuy and G E Sweeney}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-1342321749&doi=10.1016%2fj.cbpc.2003.12.002&partnerID=40&md5=2aaa377948e005b5c31c7934d672e688}, doi = {10.1016/j.cbpc.2003.12.002}, issn = {10964959}, year = {2004}, date = {2004-01-01}, journal = {Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology}, volume = {137}, number = {2}, pages = {279--284}, abstract = {Sox (SRY-related genes containing a HMG box) genes encode a family of transcription factors that are involved in a variety of developmental processes including sex determination. Twenty Sox genes are present in the genomes of humans and mice, but far less is known about the Sox gene family in other vertebrate types. We have obtained clones representing the HMG boxes of twelve Sox genes from European sea bass (Dicentrarchus labrax), a fish species whose farming is complicated by a heavily skewed sex ratio, with between 70% and 99% of offspring typically being male. The cloned Sox genes are members of the SoxB, SoxC, SoxE and SoxF groups. Sequence analysis shows that some of the clones represent genes duplicated in sea bass with respect to the mammalian Sox gene family. © 2004 Elsevier Inc. All rights reserved.}, note = {Publisher: Elsevier Inc.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Sox (SRY-related genes containing a HMG box) genes encode a family of transcription factors that are involved in a variety of developmental processes including sex determination. Twenty Sox genes are present in the genomes of humans and mice, but far less is known about the Sox gene family in other vertebrate types. We have obtained clones representing the HMG boxes of twelve Sox genes from European sea bass (Dicentrarchus labrax), a fish species whose farming is complicated by a heavily skewed sex ratio, with between 70% and 99% of offspring typically being male. The cloned Sox genes are members of the SoxB, SoxC, SoxE and SoxF groups. Sequence analysis shows that some of the clones represent genes duplicated in sea bass with respect to the mammalian Sox gene family. © 2004 Elsevier Inc. All rights reserved. |
Rigos, G; Nengas, I; Alexis, M; Athanassopoulou, F Bioavailability of oxytetracycline in sea bass, Dicentrarchus labrax (L.) Journal Article Journal of Fish Diseases, 27 , pp. 119 – 122, 2004. @article{rigos_bioavailability_2004, title = {Bioavailability of oxytetracycline in sea bass, Dicentrarchus labrax (L.)}, author = {G Rigos and I Nengas and M Alexis and F Athanassopoulou}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-1542319008&partnerID=40&md5=2fce37501284273e5a1e06048b0e5bb3}, year = {2004}, date = {2004-01-01}, journal = {Journal of Fish Diseases}, volume = {27}, pages = {119 -- 122}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Papandroulakis, N; Kentouri, M; Divanach, P Biological performance of red porgy (Pagrus pagrus) larvae under intensive rearing conditions with the use of an automated feeding system Journal Article Aquaculture International, 12 , pp. 191 – 203, 2004. @article{papandroulakis_biological_2004, title = {Biological performance of red porgy (Pagrus pagrus) larvae under intensive rearing conditions with the use of an automated feeding system}, author = {N Papandroulakis and M Kentouri and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-3042549353&partnerID=40&md5=f683700be4763c4677be7bf71340bc32}, year = {2004}, date = {2004-01-01}, journal = {Aquaculture International}, volume = {12}, pages = {191 -- 203}, abstract = {Pagrus pagrus is one of the promising species for the industry in the Mediterranean but its rearing is still far from satisfactory. In the present work, the conditions and results of larval rearing with the use of an automatic feeding system are presented. Eight populations were reared for 20 days with the ’pseudo green water’ methodology in two successive trials. Larvae were fed enriched rotifers during the entire rearing period, a mixed diet of rotifers and Artemia (Instar I when larvae reached 5 mm in length, replaced by Instar II after larvae reached 5.5 mm) and a formulated diet (after day 16 post hatching). A computerized system for feeding management was used. A total of 388,000 eggs were incubated and after 20 days, 237,973 larvae (TL = 6.96 ± 0.17 mm) were produced with a survival rate of 61.4 ± 6.3%. Individuals grew in terms of wet weight with an exponential rate of 0.167 ± 0.008 daily (R2 = 0.983) with no variations between replicates. Mean individual daily consumption at first feeding (day 4-post hatching), was 0.20 ± 0.06 mg of food (0.03 ± 0.01 dry weight), that is, 180-300 rotifers, while on day 20, consumption increased by 10 (2.081 ± 0.106 mg and 0.276 ± 0.014 in wet and dry weight, respectively). When compared with sea bream, consumption was higher by approximately 3-4-fold. The mean food conversion ratio of the experimental period was 4. In comparison with sea bream larviculture, food consumption of red porgy is higher, and a different feeding strategy is required to satisfy the requirements of the larvae. © 2004 Kluwer Academic Publishers.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Pagrus pagrus is one of the promising species for the industry in the Mediterranean but its rearing is still far from satisfactory. In the present work, the conditions and results of larval rearing with the use of an automatic feeding system are presented. Eight populations were reared for 20 days with the ’pseudo green water’ methodology in two successive trials. Larvae were fed enriched rotifers during the entire rearing period, a mixed diet of rotifers and Artemia (Instar I when larvae reached 5 mm in length, replaced by Instar II after larvae reached 5.5 mm) and a formulated diet (after day 16 post hatching). A computerized system for feeding management was used. A total of 388,000 eggs were incubated and after 20 days, 237,973 larvae (TL = 6.96 ± 0.17 mm) were produced with a survival rate of 61.4 ± 6.3%. Individuals grew in terms of wet weight with an exponential rate of 0.167 ± 0.008 daily (R2 = 0.983) with no variations between replicates. Mean individual daily consumption at first feeding (day 4-post hatching), was 0.20 ± 0.06 mg of food (0.03 ± 0.01 dry weight), that is, 180-300 rotifers, while on day 20, consumption increased by 10 (2.081 ± 0.106 mg and 0.276 ± 0.014 in wet and dry weight, respectively). When compared with sea bream, consumption was higher by approximately 3-4-fold. The mean food conversion ratio of the experimental period was 4. In comparison with sea bream larviculture, food consumption of red porgy is higher, and a different feeding strategy is required to satisfy the requirements of the larvae. © 2004 Kluwer Academic Publishers. |
Halm, S; Martínez-Rodríguez, G; Rodríguez, L; Prat, F; Mylonas, C C; Carrillo, M; Zanuy, S Cloning, characterisation, and expression of three oestrogen receptors (ERα, ERβ1 and ERβ2) in the European sea bass, Dicentrarchus labrax Journal Article Molecular and Cellular Endocrinology, 223 (1-2), pp. 63–75, 2004, ISSN: 03037207. @article{halm_cloning_2004, title = {Cloning, characterisation, and expression of three oestrogen receptors (ERα, ERβ1 and ERβ2) in the European sea bass, Dicentrarchus labrax}, author = {S Halm and G Martínez-Rodríguez and L Rodríguez and F Prat and C C Mylonas and M Carrillo and S Zanuy}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-3342941616&doi=10.1016%2fj.mce.2004.05.009&partnerID=40&md5=46ce79c38bedf1df217fcb12ab9d17c8}, doi = {10.1016/j.mce.2004.05.009}, issn = {03037207}, year = {2004}, date = {2004-01-01}, journal = {Molecular and Cellular Endocrinology}, volume = {223}, number = {1-2}, pages = {63--75}, abstract = {Three oestrogen receptor [ER] subtypes have been described in teleost fish, namely ERα, and two ERβ subtypes, called ERβ1 and ERβ2 (or ERβ and ERγ in Atlantic croaker). Their expression during embryonic development and gonadal growth has evoked interest in their potential role in sexual differentiation and gonadal development in fish. We cloned three oestrogen receptors from adult liver (sb-ERα cDNA) and ovary (partial sb-ERβ1 and sb-ERβ2 cDNAs) of the European sea bass, and according to their phylogenetic relatedness to other ERs in teleosts, named them sea bass [sb-] ERα, ERβ1 and ERβ2. Deduced amino acid numbers for sb-ERα, sb-ERβ1 and sb-ERβ2 were 639, 517 and 608, respectively, representing in the case of sb-ERβ1 and sb-ERβ2 about 90% of the open reading frame. Highest amino acid identities were found for sb-ERα with eelpout ERα (88.7%), for sb-ERβ1 with Atlantic croaker ERγ (85.8%), and for sb-ERβ2 with Atlantic croaker ERβ (90.1%). Southern analysis confirmed that all three sea bass oestrogen receptors (sb-ERs) are the products of three distinct genes. In adult sea bass, ERα was predominantly expressed in liver and pituitary, while sb-ERβ1 and sb-ERβ2 were more ubiquitously expressed, with highest expression levels in pituitary. In a mixed-sex population of juvenile sea bass, sb-ERα expression was significantly elevated in gonads at 200 days posthatch (dph), while for sb-ERβ1 and sb-ERβ2 highest expression levels were observed in gonads at 250 dph. For sb-ERβ2, expression was also significantly higher in the brain at 250 dph. The cloning of these three ER subtypes in the European sea bass together with the results obtained on expression levels in adult and juvenile animals has given us the foundation to investigate their possible role in sexual differentiation and development in this species in future studies. © 2004 Elsevier Ireland Ltd. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Three oestrogen receptor [ER] subtypes have been described in teleost fish, namely ERα, and two ERβ subtypes, called ERβ1 and ERβ2 (or ERβ and ERγ in Atlantic croaker). Their expression during embryonic development and gonadal growth has evoked interest in their potential role in sexual differentiation and gonadal development in fish. We cloned three oestrogen receptors from adult liver (sb-ERα cDNA) and ovary (partial sb-ERβ1 and sb-ERβ2 cDNAs) of the European sea bass, and according to their phylogenetic relatedness to other ERs in teleosts, named them sea bass [sb-] ERα, ERβ1 and ERβ2. Deduced amino acid numbers for sb-ERα, sb-ERβ1 and sb-ERβ2 were 639, 517 and 608, respectively, representing in the case of sb-ERβ1 and sb-ERβ2 about 90% of the open reading frame. Highest amino acid identities were found for sb-ERα with eelpout ERα (88.7%), for sb-ERβ1 with Atlantic croaker ERγ (85.8%), and for sb-ERβ2 with Atlantic croaker ERβ (90.1%). Southern analysis confirmed that all three sea bass oestrogen receptors (sb-ERs) are the products of three distinct genes. In adult sea bass, ERα was predominantly expressed in liver and pituitary, while sb-ERβ1 and sb-ERβ2 were more ubiquitously expressed, with highest expression levels in pituitary. In a mixed-sex population of juvenile sea bass, sb-ERα expression was significantly elevated in gonads at 200 days posthatch (dph), while for sb-ERβ1 and sb-ERβ2 highest expression levels were observed in gonads at 250 dph. For sb-ERβ2, expression was also significantly higher in the brain at 250 dph. The cloning of these three ER subtypes in the European sea bass together with the results obtained on expression levels in adult and juvenile animals has given us the foundation to investigate their possible role in sexual differentiation and development in this species in future studies. © 2004 Elsevier Ireland Ltd. All rights reserved. |
Polymenakou, P N; Bertilsson, S; Tselepides, A; Stephanou, E G Comparison of microbial community composition from different sediments of the Eastern Mediterranean Sea using Ŧ-RFLP, DGGE and PLFA analysis Inproceedings Barcelona, Spain, 2004, (Publication Title: 37th CIESM Congress Type: Oral Presentation). @inproceedings{polymenakou_comparison_2004, title = {Comparison of microbial community composition from different sediments of the Eastern Mediterranean Sea using Ŧ-RFLP, DGGE and PLFA analysis}, author = {P N Polymenakou and S Bertilsson and A Tselepides and E G Stephanou}, year = {2004}, date = {2004-01-01}, address = {Barcelona, Spain}, note = {Publication Title: 37th CIESM Congress Type: Oral Presentation}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Tzovenis, I; Triantaphyllidis, G; Naihong, X; Chatzinikolaou, E; Papadopoulou, K; Xouri, G; Tafas, T Cryopreservation of marine microalgae and potential toxicity of cryoprotectants to the primary steps of the aquacultural food chain Journal Article Aquaculture, 230 (1-4), pp. 457–473, 2004, ISSN: 00448486, (Publisher: Elsevier). @article{tzovenis_cryopreservation_2004, title = {Cryopreservation of marine microalgae and potential toxicity of cryoprotectants to the primary steps of the aquacultural food chain}, author = {I Tzovenis and G Triantaphyllidis and X Naihong and E Chatzinikolaou and K Papadopoulou and G Xouri and T Tafas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0842310593&doi=10.1016%2fS0044-8486%2803%2900444-7&partnerID=40&md5=2ebddbf8a832f68bfc915dcafcbffe6e}, doi = {10.1016/S0044-8486(03)00444-7}, issn = {00448486}, year = {2004}, date = {2004-01-01}, journal = {Aquaculture}, volume = {230}, number = {1-4}, pages = {457--473}, abstract = {Cryopreservation, a technique of high potential for culture collections, might offer a solution for reliable supply of microalgae in aquaculture units. Marine microalgae used in aquaculture were cryopreserved under 4, -20 and -80°C using common cryoprotectants (methanol, dimethylsulfoxide (DMSO), propylene glycol and polyvinylpyrrolidone (PVP)) with promising results for Chlorella minutissima, Chlorella stigmatophora, Isochrysis galbana and Dunaliella tertiolecta. As cryoprotectants usually are toxic above certain concentrations and exposure time, and assuming that low amounts of cryoprotectants will remain in regenerated cultures, an experimental scheme was employed to explore the lower limits of safety for these algae and their primary consumers in hatchery food chains. Results showed that methanol was well tolerated by C. stigmatophora and D. tertiolecta up to a concentration of 1.6% (v/v) while I. galbana could not survive in culture at any concentration and C. minutissima exhibited some 30% of the control's yield at 0.2%. DMSO was highly tolerated up to 1.0% by all strains with the Chlorella strains surviving well up to 2%. Propylene glycol was not only tolerated up to 8% by Dunaliella but induced mixotrophic growth as well, while for Isochrysis it was lethal at any concentration. Among zooplanktonic consumers, brine shrimp Artemia nauplii could tolerate very high concentrations of the tested cryoprotectants, the rotifer Brachionus plicatilis was found sensitive to low amounts of PVP, while the nauplii of the shrimp Penaeus japonicus and the crab Eriocheir sinensis were in general very sensitive to all cryoprotectants and in several cases to much lower amounts than 1%. However, as long as the residues of cryoprotectants are kept below 1% in the regenerated cultures, there will be no problem with the animal consumers. © 2004 Elsevier B.V. All rights reserved.}, note = {Publisher: Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {article} } Cryopreservation, a technique of high potential for culture collections, might offer a solution for reliable supply of microalgae in aquaculture units. Marine microalgae used in aquaculture were cryopreserved under 4, -20 and -80°C using common cryoprotectants (methanol, dimethylsulfoxide (DMSO), propylene glycol and polyvinylpyrrolidone (PVP)) with promising results for Chlorella minutissima, Chlorella stigmatophora, Isochrysis galbana and Dunaliella tertiolecta. As cryoprotectants usually are toxic above certain concentrations and exposure time, and assuming that low amounts of cryoprotectants will remain in regenerated cultures, an experimental scheme was employed to explore the lower limits of safety for these algae and their primary consumers in hatchery food chains. Results showed that methanol was well tolerated by C. stigmatophora and D. tertiolecta up to a concentration of 1.6% (v/v) while I. galbana could not survive in culture at any concentration and C. minutissima exhibited some 30% of the control's yield at 0.2%. DMSO was highly tolerated up to 1.0% by all strains with the Chlorella strains surviving well up to 2%. Propylene glycol was not only tolerated up to 8% by Dunaliella but induced mixotrophic growth as well, while for Isochrysis it was lethal at any concentration. Among zooplanktonic consumers, brine shrimp Artemia nauplii could tolerate very high concentrations of the tested cryoprotectants, the rotifer Brachionus plicatilis was found sensitive to low amounts of PVP, while the nauplii of the shrimp Penaeus japonicus and the crab Eriocheir sinensis were in general very sensitive to all cryoprotectants and in several cases to much lower amounts than 1%. However, as long as the residues of cryoprotectants are kept below 1% in the regenerated cultures, there will be no problem with the animal consumers. © 2004 Elsevier B.V. All rights reserved. |
Company, JB; Maiorano, P; Tselepides, A; Politou, C -Y; Plaiti, W; Rollant, G; Sardá, F Deep-sea decapod crustaceans in the western and central Mediterranean Sea: preliminary aspect of species distribution, biomass and population structure. Journal Article Scientia Marina, 68 , pp. 73–86, 2004. @article{company_deep-sea_2004, title = {Deep-sea decapod crustaceans in the western and central Mediterranean Sea: preliminary aspect of species distribution, biomass and population structure.}, author = {JB Company and P Maiorano and A Tselepides and C -Y Politou and W Plaiti and G Rollant and F Sardá}, year = {2004}, date = {2004-01-01}, journal = {Scientia Marina}, volume = {68}, pages = {73--86}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Tyrpenou, A E; Rigos, G Determination of oxolinic acid residues in gilthead seabream (Sparus aurata) muscle tissue and plasma by high-performance liquid chromatography Journal Article Chromatographia, 60 , pp. 657 – 661, 2004. @article{tyrpenou_determination_2004, title = {Determination of oxolinic acid residues in gilthead seabream (Sparus aurata) muscle tissue and plasma by high-performance liquid chromatography}, author = {A E Tyrpenou and G Rigos}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-12144273132&partnerID=40&md5=5b05f1505cf35b3cf48c9dd1fbc39a35}, year = {2004}, date = {2004-01-01}, journal = {Chromatographia}, volume = {60}, pages = {657 -- 661}, abstract = {A high-performance liquid chromatographic method for the determination of oxolinic acid (OA) residues in muscle tissue and plasma of the cultured fish gilthead seabream (Sparus aurata L.), is described. OA was extracted with ethyl acetate and after centrifugation the combined extracts were evaporated. To the remaining residue 1 mL of the mobile phase was added and the extract was partitioned with n-pentane which then was rejected by aspiration. OA was chromatographed on a Zorbax®SB-C18 column at 50°C and detected by fluorescence detection at λex 327 nm and λem 369 nm. The mobile phase was a mixture of 0.1% trifluoroacetic acid (v/v) pH 2.0 and acetonitrile-methanol 3:2 (v/v) in a combination of 50:50 (v/v) and a flow rate of 1.0 mL min-1, delivered isocratically. Method mean recovery (R%) achieved was 73.7 ± 4.4% (mean ± SD) for blank fortified samples (n = 4) range at 50, 100 and 200 μg kg-1 with a RSD = 3.3%. The limit of detection (LOD) was 2.0 μg kg-1 oxolinic acid in muscle tissue and plasma and the limit of quantification (LOQ) was 5.0 μg kg-1. The method is fast and suitable to be used with safety and accuracy for the control of OA residues in cultured seabreams and a trained analyst could carry out ready for chromatography more than 50 samples per working day.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A high-performance liquid chromatographic method for the determination of oxolinic acid (OA) residues in muscle tissue and plasma of the cultured fish gilthead seabream (Sparus aurata L.), is described. OA was extracted with ethyl acetate and after centrifugation the combined extracts were evaporated. To the remaining residue 1 mL of the mobile phase was added and the extract was partitioned with n-pentane which then was rejected by aspiration. OA was chromatographed on a Zorbax®SB-C18 column at 50°C and detected by fluorescence detection at λex 327 nm and λem 369 nm. The mobile phase was a mixture of 0.1% trifluoroacetic acid (v/v) pH 2.0 and acetonitrile-methanol 3:2 (v/v) in a combination of 50:50 (v/v) and a flow rate of 1.0 mL min-1, delivered isocratically. Method mean recovery (R%) achieved was 73.7 ± 4.4% (mean ± SD) for blank fortified samples (n = 4) range at 50, 100 and 200 μg kg-1 with a RSD = 3.3%. The limit of detection (LOD) was 2.0 μg kg-1 oxolinic acid in muscle tissue and plasma and the limit of quantification (LOQ) was 5.0 μg kg-1. The method is fast and suitable to be used with safety and accuracy for the control of OA residues in cultured seabreams and a trained analyst could carry out ready for chromatography more than 50 samples per working day. |
Chistiakov, D A; Hellemans, B; Tsigenopoulos, C S; Law, A S; Bartley, N; Bertotto, D; Libertini, A; Kotoulas, G; Haley, C S; Volckaert, F A M Development and linkage relationships for new microsatellite markers of the sea bass (Dicentrarchus labrax L.) Journal Article Animal Genetics, 35 (1), pp. 53–57, 2004, ISSN: 02689146. @article{chistiakov_development_2004, title = {Development and linkage relationships for new microsatellite markers of the sea bass (Dicentrarchus labrax L.)}, author = {D A Chistiakov and B Hellemans and C S Tsigenopoulos and A S Law and N Bartley and D Bertotto and A Libertini and G Kotoulas and C S Haley and F A M Volckaert}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-10744228402&doi=10.1046%2fj.1365-2052.2003.01076.x&partnerID=40&md5=04f787b68dff2aecc25e57226b021721}, doi = {10.1046/j.1365-2052.2003.01076.x}, issn = {02689146}, year = {2004}, date = {2004-01-01}, journal = {Animal Genetics}, volume = {35}, number = {1}, pages = {53--57}, abstract = {Twenty-eight polymorphic microsatellites were isolated from the sea bass, Dicentrarchus labrax, using a microsatellite enrichment protocol and selective hybridization with oligonucleotide probes. Analysis for these markers and 11 recently described microsatellites of D. labrax found linkage between 26 loci and revealed eight linkage groups.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Twenty-eight polymorphic microsatellites were isolated from the sea bass, Dicentrarchus labrax, using a microsatellite enrichment protocol and selective hybridization with oligonucleotide probes. Analysis for these markers and 11 recently described microsatellites of D. labrax found linkage between 26 loci and revealed eight linkage groups. |
Martinez, Brotons J; Chatzifotis, S; Divanach, P; Takeuchi, T Effect of dietary taurine supplementation on growth performance and feed selection of sea bass Dicentrarchus labrax fry fed with demand-feeders Journal Article Fisheries Science, 70 (1), pp. 74–79, 2004, ISSN: 09199268. @article{brotons_martinez_effect_2004, title = {Effect of dietary taurine supplementation on growth performance and feed selection of sea bass Dicentrarchus labrax fry fed with demand-feeders}, author = {J Brotons Martinez and S Chatzifotis and P Divanach and T Takeuchi}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-1542381102&doi=10.1111%2fj.1444-2906.2003.00773.x&partnerID=40&md5=3c8ecc80e8dfafd48e7224dbfaacd7ea}, doi = {10.1111/j.1444-2906.2003.00773.x}, issn = {09199268}, year = {2004}, date = {2004-01-01}, journal = {Fisheries Science}, volume = {70}, number = {1}, pages = {74--79}, abstract = {Two series of experiments were conducted for the evaluation of the effect of dietary taurine supplementation on survival, growth performance and feed preference of sea bass Dicentrarchus labrax fry when fed with demand-feeders a fish/soybean meal-based diet. In the first experiment, fish were imposed one of four diets supplemented with taurine (0, 0.1, 0.2 or 0.3% taurine on a dry weight basis), while in the second they had simultaneous free access to the four diets supplemented with taurine. When not allowed to choose, fish exhibited better specific growth rate of 3.2 and 3.2% per day with diets supplemented with 0.2 and 0.3% taurine than those of 2.3 and 2.2% per day of O and 0.1% taurine, respectively. When fish were free to access the diets, they selected more actively the 0.2 and 0.3% taurine-supplemented diets than the 0 and 0.1% taurine. As survival was not significantly different among trials, the results indicate that sea bass fry require a 0.2% taurine in the diet for better growth when fish meal and soybean meal are the primary sources of protein. The positive correlation between growth performance and nutrient preference is discussed as a method for improvement of fry food formulation.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Two series of experiments were conducted for the evaluation of the effect of dietary taurine supplementation on survival, growth performance and feed preference of sea bass Dicentrarchus labrax fry when fed with demand-feeders a fish/soybean meal-based diet. In the first experiment, fish were imposed one of four diets supplemented with taurine (0, 0.1, 0.2 or 0.3% taurine on a dry weight basis), while in the second they had simultaneous free access to the four diets supplemented with taurine. When not allowed to choose, fish exhibited better specific growth rate of 3.2 and 3.2% per day with diets supplemented with 0.2 and 0.3% taurine than those of 2.3 and 2.2% per day of O and 0.1% taurine, respectively. When fish were free to access the diets, they selected more actively the 0.2 and 0.3% taurine-supplemented diets than the 0 and 0.1% taurine. As survival was not significantly different among trials, the results indicate that sea bass fry require a 0.2% taurine in the diet for better growth when fish meal and soybean meal are the primary sources of protein. The positive correlation between growth performance and nutrient preference is discussed as a method for improvement of fry food formulation. |
Mandalakis, M; Zebühr, Y; Gustafsson, Ö Efficient isolation of polyaromatic fraction from aliphatic compounds in complex extracts using dimethylformamide-pentane partitionings Journal Article Journal of Chromatography A, 1041 (1-2), pp. 111–117, 2004, ISSN: 00219673. @article{mandalakis_efficient_2004, title = {Efficient isolation of polyaromatic fraction from aliphatic compounds in complex extracts using dimethylformamide-pentane partitionings}, author = {M Mandalakis and Y Zebühr and Ö Gustafsson}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-2942567872&doi=10.1016%2fj.chroma.2004.04.038&partnerID=40&md5=e5e205d42a77a25eb4e3a323824426fd}, doi = {10.1016/j.chroma.2004.04.038}, issn = {00219673}, year = {2004}, date = {2004-01-01}, journal = {Journal of Chromatography A}, volume = {1041}, number = {1-2}, pages = {111--117}, abstract = {A liquid-liquid partitioning method was optimized for the rapid and quantitative separation of polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) from aliphatic hydrocarbons in complex primary extracts. This technique was based on the selective extraction of PAHs and PCBs from an aliphatic solvent into dimethylformamide (DMF). Partition experiments demonstrated that the optimal performance was achieved with a DMF (5% H 2O)-n-pentane binary system. The optimized application of two consecutive DMF (5% H2O)-n-pentane treatments to extracts from two different polluted sediments facilitated the elimination of alkanes and unresolved complex mixture by more than 94% while the average recoveries of spiked deuterated-PAHs and 13C labeled PCBs ranged from 84 to 94 and 75 to 96%, respectively. © 2004 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A liquid-liquid partitioning method was optimized for the rapid and quantitative separation of polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) from aliphatic hydrocarbons in complex primary extracts. This technique was based on the selective extraction of PAHs and PCBs from an aliphatic solvent into dimethylformamide (DMF). Partition experiments demonstrated that the optimal performance was achieved with a DMF (5% H 2O)-n-pentane binary system. The optimized application of two consecutive DMF (5% H2O)-n-pentane treatments to extracts from two different polluted sediments facilitated the elimination of alkanes and unresolved complex mixture by more than 94% while the average recoveries of spiked deuterated-PAHs and 13C labeled PCBs ranged from 84 to 94 and 75 to 96%, respectively. © 2004 Elsevier B.V. All rights reserved. |
Mylonas, C C; Papadaki, M; Pavlidis, M; Divanach, P Evaluation of egg production and quality in the Mediterranean red porgy (Pagrus pagrus) during two consecutive spawning seasons Journal Article Aquaculture, 232 (1-4), pp. 637–649, 2004, ISSN: 00448486. @article{mylonas_evaluation_2004, title = {Evaluation of egg production and quality in the Mediterranean red porgy (Pagrus pagrus) during two consecutive spawning seasons}, author = {C C Mylonas and M Papadaki and M Pavlidis and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-1542321216&doi=10.1016%2fS0044-8486%2803%2900534-9&partnerID=40&md5=bb798427551c7d2bdbf2d0812cf57e45}, doi = {10.1016/S0044-8486(03)00534-9}, issn = {00448486}, year = {2004}, date = {2004-01-01}, journal = {Aquaculture}, volume = {232}, number = {1-4}, pages = {637--649}, abstract = {Egg production from two broodstocks of captive-reared red porgy (Pagrus pagrus) was monitored for two consecutive seasons (2001 and 2002). Spawning lasted from January to April-May under water temperature between 15.8 and 19.2°C. Spawning was mostly daily, though over the course of the two spawning seasons, the mean interval was 1.5-1.2 days spawn-1. There was a large variation in daily fecundity without a consistent trend, except for a slight peak in the middle of the spawning season. Mean (S.E.M.) annual relative fecundity ranged between 408,300 (95,800) and 442,500 (180,800) eggs kg-1 year-1. Mean fertilization success increased significantly from 36.9 (2.0)% in 2001 to 68.9 (2.5)% in 2002, resulting in mean annual viable egg production of 174,800 (87,600) and 301,500 (99,800) eggs kg-1, respectively. There were no significant differences between the 2 years in other egg quality parameters, which ranged between 74.5 and 79. 3% 1-day embryo survival, 81.6-85.5% hatching success and 76.7-78.8% 5-day larval survival. Of the relations examined, fertilization % had a significantly positive correlation with 1-day embryo survival, which in turn was significantly correlated with hatching %. Larval survival was further correlated with hatching %. These data suggest that decisions on whether to proceed with the incubation of a batch of eggs and rearing of the larvae can be made within 1 day from egg collection, before much effort is invested by the hatchery. The present study presents further information on the spawning kinetics, and egg production and quality characteristics of the Mediterranean red porgy in culture, and demonstrates the suitability of this species for reproduction in captivity. © 2004 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Egg production from two broodstocks of captive-reared red porgy (Pagrus pagrus) was monitored for two consecutive seasons (2001 and 2002). Spawning lasted from January to April-May under water temperature between 15.8 and 19.2°C. Spawning was mostly daily, though over the course of the two spawning seasons, the mean interval was 1.5-1.2 days spawn-1. There was a large variation in daily fecundity without a consistent trend, except for a slight peak in the middle of the spawning season. Mean (S.E.M.) annual relative fecundity ranged between 408,300 (95,800) and 442,500 (180,800) eggs kg-1 year-1. Mean fertilization success increased significantly from 36.9 (2.0)% in 2001 to 68.9 (2.5)% in 2002, resulting in mean annual viable egg production of 174,800 (87,600) and 301,500 (99,800) eggs kg-1, respectively. There were no significant differences between the 2 years in other egg quality parameters, which ranged between 74.5 and 79. 3% 1-day embryo survival, 81.6-85.5% hatching success and 76.7-78.8% 5-day larval survival. Of the relations examined, fertilization % had a significantly positive correlation with 1-day embryo survival, which in turn was significantly correlated with hatching %. Larval survival was further correlated with hatching %. These data suggest that decisions on whether to proceed with the incubation of a batch of eggs and rearing of the larvae can be made within 1 day from egg collection, before much effort is invested by the hatchery. The present study presents further information on the spawning kinetics, and egg production and quality characteristics of the Mediterranean red porgy in culture, and demonstrates the suitability of this species for reproduction in captivity. © 2004 Elsevier B.V. All rights reserved. |
Chatzifotis, S; Muje, P; Pavlidis, M; Ågren, J; Paalavuo, M; Mölsä, H Evolution of tissue composition and serum metabolites during gonadal development in the common dentex (Dentex dentex) Journal Article Aquaculture, 236 (1-4), pp. 557–573, 2004, ISSN: 00448486. @article{chatzifotis_evolution_2004, title = {Evolution of tissue composition and serum metabolites during gonadal development in the common dentex (Dentex dentex)}, author = {S Chatzifotis and P Muje and M Pavlidis and J Ågren and M Paalavuo and H Mölsä}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-2442560250&doi=10.1016%2fj.aquaculture.2003.12.004&partnerID=40&md5=e8411aeb1e2be9cbf5f66ae7979b840e}, doi = {10.1016/j.aquaculture.2003.12.004}, issn = {00448486}, year = {2004}, date = {2004-01-01}, journal = {Aquaculture}, volume = {236}, number = {1-4}, pages = {557--573}, abstract = {This study describes the evolution of tissue composition and serum metabolites of common dentex (Dentex dentex) in different maturity stages. During maturation, diet and perivisceral fat provide the materials needed for the development of gonads. Female and male fish had higher total proteins, total fat, triglycerides (TG) and cholesterol in their serum, at spawning and spermiation, respectively. The examination of dentex tissues (muscle, liver and gonad) revealed no statistically significant differences (Ptextless0.05) in energy density and lipid content during different maturity stages and there was no conclusive evidence that energy density and lipid content differ between sexes. In muscle, liver and gonad triglycerides fraction, the proportion of saturated fatty acids (FA) was greater than the proportion of polyunsaturated fatty acids (PUFA), whereas the reverse observation was made (with the exception of liver) in the polar lipid (PL) fraction of the above tissues. In muscle and liver, the main polyunsaturated fatty acids (n-3 PUFA) were docosahexaenoic (DHA, 22:6n-3) and eicosapentaenoic (EPA, 20:5n-3) acids. The main n-6 polyunsaturated fatty acids were linoleic (18:2n-6) and arachidonic (20:4n-6) acids. Palmitic (16:0) and oleic (18:1n-9) acids were the main saturated fatty acids and monoenes, respectively. The relative concentration of the major fatty acids varied seasonally and a pattern can be seen in both mature and immature dentex. In the PL fraction of muscle, higher values of total saturated and n-3 fatty acids, palmitic acid and DHA were obtained during summer in comparison to winter samplings; this trend was also shown by the total saturated fatty acids in the TG fraction. © 2003 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study describes the evolution of tissue composition and serum metabolites of common dentex (Dentex dentex) in different maturity stages. During maturation, diet and perivisceral fat provide the materials needed for the development of gonads. Female and male fish had higher total proteins, total fat, triglycerides (TG) and cholesterol in their serum, at spawning and spermiation, respectively. The examination of dentex tissues (muscle, liver and gonad) revealed no statistically significant differences (Ptextless0.05) in energy density and lipid content during different maturity stages and there was no conclusive evidence that energy density and lipid content differ between sexes. In muscle, liver and gonad triglycerides fraction, the proportion of saturated fatty acids (FA) was greater than the proportion of polyunsaturated fatty acids (PUFA), whereas the reverse observation was made (with the exception of liver) in the polar lipid (PL) fraction of the above tissues. In muscle and liver, the main polyunsaturated fatty acids (n-3 PUFA) were docosahexaenoic (DHA, 22:6n-3) and eicosapentaenoic (EPA, 20:5n-3) acids. The main n-6 polyunsaturated fatty acids were linoleic (18:2n-6) and arachidonic (20:4n-6) acids. Palmitic (16:0) and oleic (18:1n-9) acids were the main saturated fatty acids and monoenes, respectively. The relative concentration of the major fatty acids varied seasonally and a pattern can be seen in both mature and immature dentex. In the PL fraction of muscle, higher values of total saturated and n-3 fatty acids, palmitic acid and DHA were obtained during summer in comparison to winter samplings; this trend was also shown by the total saturated fatty acids in the TG fraction. © 2003 Elsevier B.V. All rights reserved. |
Papandroulakis, N; Suquet, M; Spedicato, M T; Machias, A; Fauvel, C; Divanach, P Feeding rates, growth performance and gametogenesis of wreckfish (Polyprion americanus) kept in captivity Journal Article Aquaculture International, 12 , pp. 395 – 407, 2004. @article{papandroulakis_feeding_2004, title = {Feeding rates, growth performance and gametogenesis of wreckfish (Polyprion americanus) kept in captivity}, author = {N Papandroulakis and M Suquet and M T Spedicato and A Machias and C Fauvel and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33749494732&partnerID=40&md5=452a6a205ec500f8fdcd8736ff3a50f1}, year = {2004}, date = {2004-01-01}, journal = {Aquaculture International}, volume = {12}, pages = {395 -- 407}, abstract = {Wreckfish (Polyprion americanus) is a panoceanic, antitropical distributed species. Despite the high market price, high growth rate, good quality flesh and the potential for aquaculture, research data are scarce. In this study, the growth performance and food intake was investigated in juvenile fish, while gametogenesis was studied in adult fish. Three groups were created from 19 individuals, which were collected during neustonic sampling, using fish aggregating devices, in association with floating objects and during tuna fishing with drift nets from Greece (Crete), Italy (Ionian Sea) and the Atlantic coast of France from September 1999 to March 2001. All individuals (initial weight between 0.5 g and 4.5kg) were reared in aquaculture facilities so as to monitor growth performance and food intake. Food (de-frozen fish - bogue, mackerel and squid - or moist pellets) was delivered ad libitum. Total length and wet weight were measured regularly. Daily food intake, the food conversion index and daily growth rate were calculated. Individuals, aging 0+, grew exceptionally fast, reaching 2.0kg (total length 50cm) in 12 months and 5.0kg (total length 65cm) in 24, while at later stages, the growth rate decreased and depended on the water temperature during rearing. Food consumption varied during the rearing period according to the developmental stage and the rearing conditions. The food conversion ratio ranged between 0.9 and 2.5 on dry weight basis during the period of the study. Gametogenesis was monitored in a separate stock of 15 individuals (11.7 ± 3.7 kg) by biopsis while blood samples were taken for steroid estimation. Increased oocyte diameter was observed in females from August to January, while plasma estradiol levels also increased from October to March. However, neither egg or sperm were collected from the stock. Young wreckfish presented a high growth rate in captivity, thus supporting the feasibility of the species for rearing. Further research, however, is required for the reproduction of the species. © 2004 Kluwer Academic Publishers.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Wreckfish (Polyprion americanus) is a panoceanic, antitropical distributed species. Despite the high market price, high growth rate, good quality flesh and the potential for aquaculture, research data are scarce. In this study, the growth performance and food intake was investigated in juvenile fish, while gametogenesis was studied in adult fish. Three groups were created from 19 individuals, which were collected during neustonic sampling, using fish aggregating devices, in association with floating objects and during tuna fishing with drift nets from Greece (Crete), Italy (Ionian Sea) and the Atlantic coast of France from September 1999 to March 2001. All individuals (initial weight between 0.5 g and 4.5kg) were reared in aquaculture facilities so as to monitor growth performance and food intake. Food (de-frozen fish - bogue, mackerel and squid - or moist pellets) was delivered ad libitum. Total length and wet weight were measured regularly. Daily food intake, the food conversion index and daily growth rate were calculated. Individuals, aging 0+, grew exceptionally fast, reaching 2.0kg (total length 50cm) in 12 months and 5.0kg (total length 65cm) in 24, while at later stages, the growth rate decreased and depended on the water temperature during rearing. Food consumption varied during the rearing period according to the developmental stage and the rearing conditions. The food conversion ratio ranged between 0.9 and 2.5 on dry weight basis during the period of the study. Gametogenesis was monitored in a separate stock of 15 individuals (11.7 ± 3.7 kg) by biopsis while blood samples were taken for steroid estimation. Increased oocyte diameter was observed in females from August to January, while plasma estradiol levels also increased from October to March. However, neither egg or sperm were collected from the stock. Young wreckfish presented a high growth rate in captivity, thus supporting the feasibility of the species for rearing. Further research, however, is required for the reproduction of the species. © 2004 Kluwer Academic Publishers. |
Mylonas, C C; Papandroulakis, N; Smboukis, A; Papadaki, M; Divanach, P Induction of spawning of cultured greater amberjack (Seriola dumerili) using GnRHa implants Journal Article Aquaculture, 237 (1-4), pp. 141–154, 2004, ISSN: 00448486. @article{mylonas_induction_2004, title = {Induction of spawning of cultured greater amberjack (Seriola dumerili) using GnRHa implants}, author = {C C Mylonas and N Papandroulakis and A Smboukis and M Papadaki and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-3142566876&doi=10.1016%2fj.aquaculture.2004.04.015&partnerID=40&md5=1d5f2c1607615bf23df2ad0e72aaa783}, doi = {10.1016/j.aquaculture.2004.04.015}, issn = {00448486}, year = {2004}, date = {2004-01-01}, journal = {Aquaculture}, volume = {237}, number = {1-4}, pages = {141--154}, abstract = {The objective of the current study was to examine the possibility of maturing Mediterranean greater amberjack (Seriola dumerili) in culture at two different facilities in Greece and inducing spawning using gonadotropin releasing-hormone agonist (GnRHa) implants. Males in both stocks (n=8) were in spermiating condition in mid June, though sperm motility was low ranging between 5% and 30%, while motility duration ranged between 2.1 and 2.5 min. Females of the first stock (n=4) at the same time had ovaries with either primary oocytes or early vitellogenic oocytes at advanced stages of atresia, indicating a failure in oogenesis. On the contrary, the only female of the second stock contained oocytes at all stages of oogenesis, including early final oocyte maturation (FOM; oocyte diameter 650 μm). Implantation with a GnRHa implant induced the first spawn about 36 h later. In the absence of another spawn, a gonadal biopsy was taken 15 days later, indicating the existence of more oocytes at post vitellogenesis and some with almost complete lipid droplet coalescence. A second GnRHa implantation at this time resulted in consecutive egg releases after 36 h, 4 and 5 days, with fertilized eggs only at 36 h and 5 days. The study underlines the existence of important reproductive dysfunctions of greater amberjack in captivity, and demonstrates that GnRHa implants can be used for the induction of multiple spawns of viable eggs. © 2004 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The objective of the current study was to examine the possibility of maturing Mediterranean greater amberjack (Seriola dumerili) in culture at two different facilities in Greece and inducing spawning using gonadotropin releasing-hormone agonist (GnRHa) implants. Males in both stocks (n=8) were in spermiating condition in mid June, though sperm motility was low ranging between 5% and 30%, while motility duration ranged between 2.1 and 2.5 min. Females of the first stock (n=4) at the same time had ovaries with either primary oocytes or early vitellogenic oocytes at advanced stages of atresia, indicating a failure in oogenesis. On the contrary, the only female of the second stock contained oocytes at all stages of oogenesis, including early final oocyte maturation (FOM; oocyte diameter 650 μm). Implantation with a GnRHa implant induced the first spawn about 36 h later. In the absence of another spawn, a gonadal biopsy was taken 15 days later, indicating the existence of more oocytes at post vitellogenesis and some with almost complete lipid droplet coalescence. A second GnRHa implantation at this time resulted in consecutive egg releases after 36 h, 4 and 5 days, with fertilized eggs only at 36 h and 5 days. The study underlines the existence of important reproductive dysfunctions of greater amberjack in captivity, and demonstrates that GnRHa implants can be used for the induction of multiple spawns of viable eggs. © 2004 Elsevier B.V. All rights reserved. |
Publications
2005 |
Physiological Genomics, 23 (2), pp. 182–191, 2005, ISSN: 10948341. |
Journal of Fish Biology, 66 (4), pp. 938–956, 2005, ISSN: 00221112, (Publisher: Blackwell Publishing Ltd). |
Impact of aquaculture on the genetic structure of mediterranean populations of Dicentrarchus labrax Journal Article Aquatic Living Resources, 18 (1), pp. 71–76, 2005, ISSN: 0990-7440, 1765-2952, (Original title (in French): Impact de l'élevage sur la structure génétique des populations méditerranéennes de Dicentrarchus labrax). |
Induction of ovulation and spawning in the Mediterranean Red Porgy, Pagrus Pagrus, by controlled delivery and acute injection of GnRHa Journal Article Israeli Journal of Aquaculture - Bamidgeh, 57 (4), pp. 223–230, 2005, ISSN: 0792156X. |
Journal of Fish Biology, 67 (3), pp. 652–668, 2005, ISSN: 00221112. |
Isolation and characterization of polymorphic microsatellite markers in the wall lizard Podarcis erhardii (Squamata: Lacertidae) Journal Article Molecular Ecology Notes, 5 (3), pp. 549–551, 2005. |
Isolation and characterization of six polymorphic microsatellite markers in the freshwater turtle Mauremys rivulata (Testudines: Geoemydidae) Journal Article Molecular Ecology Notes, 5 (4), pp. 727–729, 2005. |
Isolation, characterization and sequence determination of Noda virus from sea bass (Dicentrarchus labrax L.) reared in freshwater and marine facilities in Greece Journal Article Bulletin of the Hellenic Veterinary Medical Society, 56 (2), pp. 105–113, 2005. |
Mass budget and dynamics of polychlorinated biphenyls in the eastern Mediterranean Sea Journal Article Global Biogeochemical Cycles, 19 (3), pp. 1–16, 2005, ISSN: 08866236. |
Mediterranean lagoons revisited: Weakness and efficiency of the rapid biodiversity assessment techniques in a severely fluctuating environment Journal Article Biodiversity and Conservation, 14 (10), pp. 2347–2359, 2005, ISSN: 09603115. |
Modeling feeding processes: A test of a new model for sea bream (Sparus aurata) larvae Journal Article Canadian Journal of Fisheries and Aquatic Sciences, 62 , pp. 425 – 435, 2005. |
Bulletin of the European Association of Fish Pathologists, 25 (1), pp. 28–31, 2005, ISSN: 01080288. |
On the analysis of black carbon in sediments: Evaluation of field data for geochemical consistency Inproceedings Geophysical Research Abstracts, pp. EGU05–A–06089, 2005. |
Ontogeny of the shi drum Umbrina cirrosa (Linnaeus 1758), a candidate new species for aquaculture Journal Article Aquaculture Research, 36 (13), pp. 1265–1272, 2005, ISSN: 1355557X. |
Ontogeny of the thyroid hormones and cortisol in the gilthead sea bream, Sparus aurata Journal Article General and Comparative Endocrinology, 142 , pp. 186 – 192, 2005. |
Phylogenetic relationships and evolutionary history of snake-eyed skink Ablepharus kitaibelii (Sauria: Scincidae) Journal Article Molecular Phylogenetics and Evolution, 34 , pp. 245 – 256, 2005. |
Preliminary results on the effect of otter trawling on hyperbenthic communities in Heraklion Bay (Eastern Mediterranean, Cretan Sea) Journal Article American Fisheries Society Symposium, 41 , pp. 529–537, 2005. |
Bulletin of the European Association of Fish Pathologists, 25 (6), pp. 256–261, 2005, ISSN: 01080288. |
Continental Shelf Research, 25 , pp. 2196–2212, 2005. |
Successional stages of experimental artificial reefs deployed in Vistonikos gulf (North SAegean Sea, Greece): Preliminary results Journal Article Belgian Journal of Zoology, 135 , pp. 209–215, 2005. |
Supercritical free-surface turbulent flow over a semicircular obstacle Inproceedings pp. 1366–1376, 2005, (Publication Title: XXXI IAHR Congress). |
The effect of different carotenoid sources on skin coloration of cultured red porgy (Pagrus pagrus) Journal Article Aquaculture Research, 36 (15), pp. 1517–1525, 2005, ISSN: 1355557X. |
The effect of different types of otter trawl groundrope on benthic nutrient fluxes and sediment biogeochemistry Journal Article American Fisheries Society Symposium, 41 , pp. 539–544, 2005. |
The phylogeography of the gecko Cyrtopodion kotschyi (Reptilia: Gekkonidae) in the Aegean archipelago Journal Article Molecular Phylogenetics and Evolution, 35 (3), pp. 612–623, 2005, ISSN: 10557903. |
Molecular Phylogenetics and Evolution, 34 (1), pp. 55–66, 2005, ISSN: 10557903. |
The use of the polychaete worm Nereis virens eggs as vehicle for the delivery of oxytetracycline in Solea solea larvae Journal Article Aquaculture, 243 (1-4), pp. 1–7, 2005, ISSN: 00448486. |
2004 |
Sensory, microbiological, and chemical spoilage of cultured common sea bass ( Dicentrarchus labrax) stored in ice: a seasonal differentiation Journal Article European Food Research and Technology, 219 (6), pp. 584–587, 2004, ISSN: 1438-2377, 1438-2385. |
Development of a reference diet for use in indispensable amino acid requirement studies of gilthead seabream Sparus aurata L. Journal Article Aquaculture Nutrition, 10 (5), pp. 335–343, 2004, ISSN: 1353-5773, 1365-2095. |
Mortality control of VNN disease in 0+ grouper, Epinephelus marginatus after prolonged bath in dense Chlorella minutissima culture. Inproceedings EAS, (Ed.): European Aquaculture Society, Biotechnologies for Quality, Barcelona, Spain, 2004. |
The rearing of common dentex Dentex dentex (L.) During the hatchery and on-growing phases Journal Article Aquaculture, 240 (1-4), pp. 165–173, 2004, ISSN: 00448486. |
Floating Breakwater Response to Waves Action Using a Boussinesq Model Coupled with a 2DV Elliptic Solver Journal Article Journal of Waterway, Port, Coastal, and Ocean Engineering, 130 (5), pp. 243–255, 2004, ISSN: 0733-950X, 1943-5460. |
Stock discrimination and temporal and spatial genetic variation of sardine (Sardina pilchardus) in northeastern Atlantic, with a combined analysis of nuclear (microsatellites and allozymes) and mitochondrial DNA markers. Inproceedings Kasapidis, P (Ed.): 2004 ICES Annual Science Conference, 2004. |
Aquaculture, 232 (1-4), pp. 407–424, 2004, ISSN: 00448486. |
Genetic comparison of wild and cultivated European populations of the gilthead sea bream (Sparus aurata) Journal Article Aquaculture, 230 (1-4), pp. 65–80, 2004, ISSN: 00448486. |
Accumulation of ivermectin in the brain of sea bream, Sparus aurata after intraperitoneal administration Journal Article Environmental Toxicology and Pharmacology, 17 (1), pp. 9–12, 2004, ISSN: 13826689. |
Analysis of the Sox gene family in the European sea bass (Dicentrarchus labrax) Journal Article Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 137 (2), pp. 279–284, 2004, ISSN: 10964959, (Publisher: Elsevier Inc.). |
Bioavailability of oxytetracycline in sea bass, Dicentrarchus labrax (L.) Journal Article Journal of Fish Diseases, 27 , pp. 119 – 122, 2004. |
Biological performance of red porgy (Pagrus pagrus) larvae under intensive rearing conditions with the use of an automated feeding system Journal Article Aquaculture International, 12 , pp. 191 – 203, 2004. |
Cloning, characterisation, and expression of three oestrogen receptors (ERα, ERβ1 and ERβ2) in the European sea bass, Dicentrarchus labrax Journal Article Molecular and Cellular Endocrinology, 223 (1-2), pp. 63–75, 2004, ISSN: 03037207. |
Comparison of microbial community composition from different sediments of the Eastern Mediterranean Sea using Ŧ-RFLP, DGGE and PLFA analysis Inproceedings Barcelona, Spain, 2004, (Publication Title: 37th CIESM Congress Type: Oral Presentation). |
Cryopreservation of marine microalgae and potential toxicity of cryoprotectants to the primary steps of the aquacultural food chain Journal Article Aquaculture, 230 (1-4), pp. 457–473, 2004, ISSN: 00448486, (Publisher: Elsevier). |
Deep-sea decapod crustaceans in the western and central Mediterranean Sea: preliminary aspect of species distribution, biomass and population structure. Journal Article Scientia Marina, 68 , pp. 73–86, 2004. |
Determination of oxolinic acid residues in gilthead seabream (Sparus aurata) muscle tissue and plasma by high-performance liquid chromatography Journal Article Chromatographia, 60 , pp. 657 – 661, 2004. |
Development and linkage relationships for new microsatellite markers of the sea bass (Dicentrarchus labrax L.) Journal Article Animal Genetics, 35 (1), pp. 53–57, 2004, ISSN: 02689146. |
Effect of dietary taurine supplementation on growth performance and feed selection of sea bass Dicentrarchus labrax fry fed with demand-feeders Journal Article Fisheries Science, 70 (1), pp. 74–79, 2004, ISSN: 09199268. |
Efficient isolation of polyaromatic fraction from aliphatic compounds in complex extracts using dimethylformamide-pentane partitionings Journal Article Journal of Chromatography A, 1041 (1-2), pp. 111–117, 2004, ISSN: 00219673. |
Evaluation of egg production and quality in the Mediterranean red porgy (Pagrus pagrus) during two consecutive spawning seasons Journal Article Aquaculture, 232 (1-4), pp. 637–649, 2004, ISSN: 00448486. |
Evolution of tissue composition and serum metabolites during gonadal development in the common dentex (Dentex dentex) Journal Article Aquaculture, 236 (1-4), pp. 557–573, 2004, ISSN: 00448486. |
Feeding rates, growth performance and gametogenesis of wreckfish (Polyprion americanus) kept in captivity Journal Article Aquaculture International, 12 , pp. 395 – 407, 2004. |
Induction of spawning of cultured greater amberjack (Seriola dumerili) using GnRHa implants Journal Article Aquaculture, 237 (1-4), pp. 141–154, 2004, ISSN: 00448486. |