2000 |
Koumoundouros, G; Divanach, P; Savaki, A; Kentouri, M Effects of three preservation methods on the evolution of swimbladder radiographic appearance in sea bass and sea bream juveniles Journal Article Aquaculture, 182 , pp. 17 – 25, 2000. @article{koumoundouros_effects_2000, title = {Effects of three preservation methods on the evolution of swimbladder radiographic appearance in sea bass and sea bream juveniles}, author = {G Koumoundouros and P Divanach and A Savaki and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034142216&partnerID=40&md5=676bd624fd0f9054b964b990f7787846}, doi = {https://doi.org/10.1016/S0044-8486(99)00249-5}, year = {2000}, date = {2000-01-01}, journal = {Aquaculture}, volume = {182}, pages = {17 -- 25}, abstract = {Sea bass and sea bream juveniles with functional swimbladder were exposed to three methods of preservation: (a) in 10% phosphate buffered formalin, (b) at 4°C, and (c) at -25°C. They were then X-rayed daily over 10 days to evaluate the impact of these methods on the evolution of the radiographic image. All methods significantly decreased the apparent length and depth of the swimbladder, but with different intensities. Freezing had the least effect (9% and 12% reduction in bream and bass, respectively) and only during the first day of preservation. Four days of formalin fixation led to the total disappearance of this image. Preservation at 4°C led to the autolysis and putrefaction of the internal organs which was responsible for the poor images after three days. The results were discussed in terms of their use for the assessment of Juvenile quality by radiography.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Sea bass and sea bream juveniles with functional swimbladder were exposed to three methods of preservation: (a) in 10% phosphate buffered formalin, (b) at 4°C, and (c) at -25°C. They were then X-rayed daily over 10 days to evaluate the impact of these methods on the evolution of the radiographic image. All methods significantly decreased the apparent length and depth of the swimbladder, but with different intensities. Freezing had the least effect (9% and 12% reduction in bream and bass, respectively) and only during the first day of preservation. Four days of formalin fixation led to the total disappearance of this image. Preservation at 4°C led to the autolysis and putrefaction of the internal organs which was responsible for the poor images after three days. The results were discussed in terms of their use for the assessment of Juvenile quality by radiography. |
Pavlidis, M; Koumoundouros, G; Sterioti, A; Somarakis, S; Divanach, P; Kentouri, M Evidence of temperature-dependent sex determination in the european sea bass (Dicentrarchus labrax L.) Journal Article Journal of Experimental Zoology, 287 , pp. 225 – 232, 2000. @article{pavlidis_evidence_2000, title = {Evidence of temperature-dependent sex determination in the european sea bass (Dicentrarchus labrax L.)}, author = {M Pavlidis and G Koumoundouros and A Sterioti and S Somarakis and P Divanach and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034255757&partnerID=40&md5=2d194d1edd776c20c4fe6c96e412b855}, year = {2000}, date = {2000-01-01}, journal = {Journal of Experimental Zoology}, volume = {287}, pages = {225 -- 232}, abstract = {To test the hypothesis that sex determination in the European sea bass (Dicentrarchus labrax L.) can be affected by the incubating temperature during the very early developmental stages, eggs from the same batch of spontaneously spawned broodstock were divided at the stage of half-epiboly into three groups according to rearing temperature: G13 = 13°C}, keywords = {}, pubstate = {published}, tppubtype = {article} } To test the hypothesis that sex determination in the European sea bass (Dicentrarchus labrax L.) can be affected by the incubating temperature during the very early developmental stages, eggs from the same batch of spontaneously spawned broodstock were divided at the stage of half-epiboly into three groups according to rearing temperature: G13 = 13°C |
Papandroulakis, N; Markakis, G; Divanach, P; Kentouri, M Feeding requirements of sea bream (Sparus aurata) larvae under intensive rearing conditions: Development of a fuzzy logic controller for feeding Journal Article Aquacultural Engineering, 21 , pp. 285 – 299, 2000. @article{papandroulakis_feeding_2000, title = {Feeding requirements of sea bream (Sparus aurata) larvae under intensive rearing conditions: Development of a fuzzy logic controller for feeding}, author = {N Papandroulakis and G Markakis and P Divanach and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034008999&partnerID=40&md5=08b95efaa79a087e5c80a9df9872afc1}, year = {2000}, date = {2000-01-01}, journal = {Aquacultural Engineering}, volume = {21}, pages = {285 -- 299}, abstract = {Applied methods for larval rearing suffer from the difficulty of accurately predicting larvae quantitative feeding requirements for optimum growth. Even when known, requirements change according to the behavior of each population reared. Computer based feeding systems require tools for adapting the delivered amounts of food to the needs of the reared population. In this work the daily feeding requirements of sea bream (Sparus aurata) larvae were estimated, and based on these estimations a fuzzy logic controller (FLC) for feeding was designed and developed. Sea bream larvae were reared, in triplicate, under intensive conditions in a pilot scale rearing system for 20 days with the pseudo-green water method. Larvae reached 6.7 ± 0.6 mm (mean value and S.D.) at the end of the experimental period while the mean survival of the populations was 48.0 ± 12.6%. Consumption was 0.017 ± 0.009 mg of carbon per individual at first feeding and increased to 0.198 ± 0.032 mg at day 20 post-hatching. The findings were used for the development of a FLC for feeding that adapts the amount of food delivered to the larvae according to changes in the reared population. The FLC utilizes five linguistic variables describing the state of the population and a rule base composed of 316 rules. The developed FLC allows the use of computer based feeding systems during rearing of early larval stages. (C) 2000 Elsevier Science B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Applied methods for larval rearing suffer from the difficulty of accurately predicting larvae quantitative feeding requirements for optimum growth. Even when known, requirements change according to the behavior of each population reared. Computer based feeding systems require tools for adapting the delivered amounts of food to the needs of the reared population. In this work the daily feeding requirements of sea bream (Sparus aurata) larvae were estimated, and based on these estimations a fuzzy logic controller (FLC) for feeding was designed and developed. Sea bream larvae were reared, in triplicate, under intensive conditions in a pilot scale rearing system for 20 days with the pseudo-green water method. Larvae reached 6.7 ± 0.6 mm (mean value and S.D.) at the end of the experimental period while the mean survival of the populations was 48.0 ± 12.6%. Consumption was 0.017 ± 0.009 mg of carbon per individual at first feeding and increased to 0.198 ± 0.032 mg at day 20 post-hatching. The findings were used for the development of a FLC for feeding that adapts the amount of food delivered to the larvae according to changes in the reared population. The FLC utilizes five linguistic variables describing the state of the population and a rule base composed of 316 rules. The developed FLC allows the use of computer based feeding systems during rearing of early larval stages. (C) 2000 Elsevier Science B.V. |
Castritsi-Catharios, ; Kotzamanis, I Fish farming design Incollection Castritsi-Catharios, J (Ed.): Current knowledge in Aquaculture, Students’ Handbook, pp. Β 1–11, University publications, Athens, 2000, (Backup Publisher: University publications). @incollection{castritsi-catharios_fish_2000, title = {Fish farming design}, author = {Castritsi-Catharios and I Kotzamanis}, editor = {J Castritsi-Catharios}, year = {2000}, date = {2000-01-01}, booktitle = {Current knowledge in Aquaculture, Students’ Handbook}, pages = {Β 1--11}, publisher = {University publications}, address = {Athens}, series = {Modern Trends in Aquaculture}, note = {Backup Publisher: University publications}, keywords = {}, pubstate = {published}, tppubtype = {incollection} } |
Navajas, M; Tsagkarakov, A; Lagnel, J; Perrot-Minnot, M -J Genetic differentiation in Tetranychus urticae (Acari: Tetranychidae): Polymorphism, host races or sibling species? Journal Article Experimental and Applied Acarology, 24 , pp. 365 – 376, 2000. @article{navajas_genetic_2000, title = {Genetic differentiation in Tetranychus urticae (Acari: Tetranychidae): Polymorphism, host races or sibling species?}, author = {M Navajas and A Tsagkarakov and J Lagnel and M -J Perrot-Minnot}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0033665460&partnerID=40&md5=2ee7e5fb6a27402605860fa294ee99eb}, year = {2000}, date = {2000-01-01}, journal = {Experimental and Applied Acarology}, volume = {24}, pages = {365 -- 376}, abstract = {Based on allozyme electrophoresis at the Pgm locus and nuclear ribosomal DNA (ITS2) sequences, we studied the genetic variation of the two-spotted spider mite Tetranychus urticae Koch collected on rose bay, Nerium oleander L. (Apocynaceae), from several localities around the Mediterranean basin. In addition, we compared these results with those of Navajas et al. (1998) and Tsagkarakou (1997) who collected from several other host plants from the Mediterranean. In the western part of this area (Spain, France, Tunisia), we found the individuals collected from rose bay to be clearly genetically differentiated from other samples. No evidence of such host-associated differentiation was detected in the eastern Mediterranean (Italy and Greece), The genetic differentiation of mites collected on rose bay was investigated further by studying the reproductive incompatibilities between populations in Greece and in France and a laboratory strain reared on bean, Phaseolus vulgaris, in France. Reciprocal crosses performed between these strains revealed variable levels of incompatibility, spanning from partial to complete reproductive isolation. In all cases incompatibility was asymmetric. We designed a test based on double-mating to establish the fertilization status of females in fully incompatible crosses. These crosses showed that the females had been inseminated, which suggests that the barrier to reproduction is not of a prezygotic behavioral nature. The data raises the question of the relative role of ecological factors (host plant) and geographical distance, in the ongoing differentiation process potentially leading to speciation.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Based on allozyme electrophoresis at the Pgm locus and nuclear ribosomal DNA (ITS2) sequences, we studied the genetic variation of the two-spotted spider mite Tetranychus urticae Koch collected on rose bay, Nerium oleander L. (Apocynaceae), from several localities around the Mediterranean basin. In addition, we compared these results with those of Navajas et al. (1998) and Tsagkarakou (1997) who collected from several other host plants from the Mediterranean. In the western part of this area (Spain, France, Tunisia), we found the individuals collected from rose bay to be clearly genetically differentiated from other samples. No evidence of such host-associated differentiation was detected in the eastern Mediterranean (Italy and Greece), The genetic differentiation of mites collected on rose bay was investigated further by studying the reproductive incompatibilities between populations in Greece and in France and a laboratory strain reared on bean, Phaseolus vulgaris, in France. Reciprocal crosses performed between these strains revealed variable levels of incompatibility, spanning from partial to complete reproductive isolation. In all cases incompatibility was asymmetric. We designed a test based on double-mating to establish the fertilization status of females in fully incompatible crosses. These crosses showed that the females had been inseminated, which suggests that the barrier to reproduction is not of a prezygotic behavioral nature. The data raises the question of the relative role of ecological factors (host plant) and geographical distance, in the ongoing differentiation process potentially leading to speciation. |
Hatziyanni, E; Tsapakis, E; Plaiti, W; Karakassis, I Impact of organic enrichment on the vertical distribution and the size of macrofauna in a coastal fish farming size. Inproceedings pp. 341–345, Chios, Greece, 2000, (Publication Title: The 6th Hellenic Symposium of Oceanography and Fisheries,). @inproceedings{hatziyanni_impact_2000, title = {Impact of organic enrichment on the vertical distribution and the size of macrofauna in a coastal fish farming size.}, author = {E Hatziyanni and E Tsapakis and W Plaiti and I Karakassis}, year = {2000}, date = {2000-01-01}, volume = {2}, pages = {341--345}, address = {Chios, Greece}, note = {Publication Title: The 6th Hellenic Symposium of Oceanography and Fisheries,}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Perrot-Minnot, Marie-Jeanne; Lagnel, Jacques; Desmarais, Eric; Navajas, Maria Experimental and Applied Acarology, 24 (10/11), pp. 795–803, 2000, ISSN: 01688162. @article{perrot-minnot_isolation_2000, title = {Isolation and characterization by direct amplification of length polymorphisms (DALP) of codominant genetic markers with mendelian inheritance in Neoseiulus californicus (Acari : Phytoseiidae)}, author = {Marie-Jeanne Perrot-Minnot and Jacques Lagnel and Eric Desmarais and Maria Navajas}, url = {http://link.springer.com/10.1023/A:1006457310697}, doi = {10.1023/A:1006457310697}, issn = {01688162}, year = {2000}, date = {2000-01-01}, urldate = {2020-08-18}, journal = {Experimental and Applied Acarology}, volume = {24}, number = {10/11}, pages = {795--803}, abstract = {We report the first application of a new method designed to isolate polymorphic loci in any organism, the direct amplification of length polymorphism. Five polymorphic loci were readily isolated in the predatory mite Neoseiulus californicus (Acari: Phytoseiidae). Two to five alleles were identified among 46 isofemale lines based on fragment size variation due to micro-deletions/insertions. Genotyping F1 and F2 progenies from controlled heterogametic crosses and backcrosses allowed to establish the Mendelian inheritance of these alleles, their codominance, and pairwise recombination rates. Nucleotidic sequence divergence due to single base substitution was also found in the flanking regions of the polymorphism. We discuss the usefulness of these markers in studies of reproductive systems as well as population genetics, in particular, in mite species where the amount of DNA or richness in microsatellites could be limiting factors in the isolation of polymorphic loci.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We report the first application of a new method designed to isolate polymorphic loci in any organism, the direct amplification of length polymorphism. Five polymorphic loci were readily isolated in the predatory mite Neoseiulus californicus (Acari: Phytoseiidae). Two to five alleles were identified among 46 isofemale lines based on fragment size variation due to micro-deletions/insertions. Genotyping F1 and F2 progenies from controlled heterogametic crosses and backcrosses allowed to establish the Mendelian inheritance of these alleles, their codominance, and pairwise recombination rates. Nucleotidic sequence divergence due to single base substitution was also found in the flanking regions of the polymorphism. We discuss the usefulness of these markers in studies of reproductive systems as well as population genetics, in particular, in mite species where the amount of DNA or richness in microsatellites could be limiting factors in the isolation of polymorphic loci. |
Koutsoubas, D; Dounas, C; Arvanitidis, C; Kornilios, S; Petihakis, G; Triantafyllou, G; Eleftheriou, A Macrobenthic community structure and disturbance assessment in Gialova Lagoon, Ionian Sea Journal Article ICES Journal of Marine Science, 57 (5), pp. 1472–1480, 2000, ISSN: 10543139, (Publisher: Academic Press). @article{koutsoubas_macrobenthic_2000, title = {Macrobenthic community structure and disturbance assessment in Gialova Lagoon, Ionian Sea}, author = {D Koutsoubas and C Dounas and C Arvanitidis and S Kornilios and G Petihakis and G Triantafyllou and A Eleftheriou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034493473&doi=10.1006%2fjmsc.2000.0905&partnerID=40&md5=ab96a84ab0a1dbac9ddf62aba60edc11}, doi = {10.1006/jmsc.2000.0905}, issn = {10543139}, year = {2000}, date = {2000-01-01}, journal = {ICES Journal of Marine Science}, volume = {57}, number = {5}, pages = {1472--1480}, abstract = {Gialova Lagoon, a coastal marine ecosystem in the Ionian Sea, suffered the impact of an oil spill incident in October 1993, leading to the extensive fish deaths when the oil tanker "Iliad" hit bottom in the entrance of the neighbouring Navarino Bay. A multidisciplinary study investigating the structure and functioning of Gialova Lagoon for the development of an integrated economic, social, and environmental management policy consistent with its conservation was carried out on a seasonal basis during 1995/1996. One of the research priorities was to investigate the structure and dynamics of the macrozoobenthic communities and assess whether these communities had recovered from the impact of the oil spill. The various methods applied (abundance/biomass comparison, distribution of species in geometric abundance and geometric size classes) revealed no detectable disturbance of the macrobenthic communities due to anthropogenic impact. However, it was proved that the lagoonal macrofauna suffered extreme natural disturbance due to a dystrophic crisis that occurred during autumn although they successfully recovered in subsequent seasons. Different sets of environmental factors were found to be highly correlated with the temporal and spatial distribution pattern of the macrobenthic communities and are discussed in detail. The coenocline observed in Gialova Lagoon appears to be strongly correlated with the degree of water exchange with adjacent marine and continental environments. © 2000 International Council for the Exploration of the Sea.}, note = {Publisher: Academic Press}, keywords = {}, pubstate = {published}, tppubtype = {article} } Gialova Lagoon, a coastal marine ecosystem in the Ionian Sea, suffered the impact of an oil spill incident in October 1993, leading to the extensive fish deaths when the oil tanker "Iliad" hit bottom in the entrance of the neighbouring Navarino Bay. A multidisciplinary study investigating the structure and functioning of Gialova Lagoon for the development of an integrated economic, social, and environmental management policy consistent with its conservation was carried out on a seasonal basis during 1995/1996. One of the research priorities was to investigate the structure and dynamics of the macrozoobenthic communities and assess whether these communities had recovered from the impact of the oil spill. The various methods applied (abundance/biomass comparison, distribution of species in geometric abundance and geometric size classes) revealed no detectable disturbance of the macrobenthic communities due to anthropogenic impact. However, it was proved that the lagoonal macrofauna suffered extreme natural disturbance due to a dystrophic crisis that occurred during autumn although they successfully recovered in subsequent seasons. Different sets of environmental factors were found to be highly correlated with the temporal and spatial distribution pattern of the macrobenthic communities and are discussed in detail. The coenocline observed in Gialova Lagoon appears to be strongly correlated with the degree of water exchange with adjacent marine and continental environments. © 2000 International Council for the Exploration of the Sea. |
Huvet, Arnaud; Lapègue, S; Magoulas, A; Boudry, P Mitochondrial and nuclear DNA phylogeography of Crassostrea angulata, the Portuguese oyster endangered in Europe Journal Article Conservation Genetics, 1 (3), pp. 251–262, 2000, ISSN: 15660621. @article{huvet_mitochondrial_2000, title = {Mitochondrial and nuclear DNA phylogeography of Crassostrea angulata, the Portuguese oyster endangered in Europe}, author = {Arnaud Huvet and S Lapègue and A Magoulas and P Boudry}, url = {http://link.springer.com/10.1023/A:1011505805923}, doi = {10.1023/A:1011505805923}, issn = {15660621}, year = {2000}, date = {2000-01-01}, urldate = {2020-08-09}, journal = {Conservation Genetics}, volume = {1}, number = {3}, pages = {251--262}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Tsigenopoulos, C S; Berrebi, P Molecular Phylogenetics and Evolution, 14 , pp. 165 – 179, 2000. @article{tsigenopoulos_molecular_2000, title = {Molecular phylogeny of north Mediterranean freshwater barbs (genus Barbus: Cyprinidae) inferred from cytochrome b sequences: Biogeographic and systematic implications}, author = {C S Tsigenopoulos and P Berrebi}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034029971&partnerID=40&md5=ace051f20e05e83cd39f80b54a10d813}, year = {2000}, date = {2000-01-01}, journal = {Molecular Phylogenetics and Evolution}, volume = {14}, pages = {165 -- 179}, abstract = {We investigated phylogenetic relationships among north Mediterranean species of the genus Barbus using sequences of the cytochrome b gene. Our results indicate that the species belong to two major clades that are consistent with those previously defined from morphological features. The first clade includes species ranging from France to the Black Sea. In this clade, there is a well-supported monophyletic group of large-sized fluvio-lacustrine barbs; however, the monophyly of the small-sized rheophilic species is not clear. The second clade comprises species found in Spain, Greece, and Asia Minor and probably represents the oldest group present in the north Mediterranean rivers. In general, there is good concordance between geography and phylogenetic relationships. These results are compared to those from previous morphological- and allozyme-based studies and demonstrate widespread discordance and polyphyly in the traditional taxonomy of the genus Barbus. This study is one of the first reporting the phylogenetic and biogeographic relationships of a genus that is widely distributed in European rivers and contains species that are a major component of the European ichthyofauna. (C) 2000 Academic Press.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We investigated phylogenetic relationships among north Mediterranean species of the genus Barbus using sequences of the cytochrome b gene. Our results indicate that the species belong to two major clades that are consistent with those previously defined from morphological features. The first clade includes species ranging from France to the Black Sea. In this clade, there is a well-supported monophyletic group of large-sized fluvio-lacustrine barbs; however, the monophyly of the small-sized rheophilic species is not clear. The second clade comprises species found in Spain, Greece, and Asia Minor and probably represents the oldest group present in the north Mediterranean rivers. In general, there is good concordance between geography and phylogenetic relationships. These results are compared to those from previous morphological- and allozyme-based studies and demonstrate widespread discordance and polyphyly in the traditional taxonomy of the genus Barbus. This study is one of the first reporting the phylogenetic and biogeographic relationships of a genus that is widely distributed in European rivers and contains species that are a major component of the European ichthyofauna. (C) 2000 Academic Press. |
Arvanitidis, C Polychaete fauna of the Aegean Sea: Inventory and new information Journal Article Bulletin of Marine Science, 66 (1), pp. 73–96, 2000, ISSN: 00074977, (Publisher: Rosenstiel School of Marine and Atmospheric Science). @article{arvanitidis_polychaete_2000, title = {Polychaete fauna of the Aegean Sea: Inventory and new information}, author = {C Arvanitidis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034580549&partnerID=40&md5=2f5e5caaa482887d362e82dfe1ece550}, issn = {00074977}, year = {2000}, date = {2000-01-01}, journal = {Bulletin of Marine Science}, volume = {66}, number = {1}, pages = {73--96}, abstract = {Examination of collections from the Aegean Sea and a review of the literature revealed that 592 polychaete species have been recorded from this area. Five of the species identified are reported for the first time from the Mediterranean and the Black Sea, 38 species are new records for the Eastern Mediterranean and 55 are new elements for the Aegean polychaete fauna. Information on the habitat and the geographic distribution of the species newly reported from the Eastern Mediterranean is given. The checklist of the Aegean Polychaeta is presented. Although being located in the vicinity of the Eastern Mediterranean province and the Black Sea which are characterized as faunistically impoverished areas, the Aegean hosts a rich polychaete fauna, second in order in species diversity after the Western Mediterranean. The latter demonstrates the importance of this area in the understanding the patterns of polychaete distribution along the Mediterranean and the Black Sea.}, note = {Publisher: Rosenstiel School of Marine and Atmospheric Science}, keywords = {}, pubstate = {published}, tppubtype = {article} } Examination of collections from the Aegean Sea and a review of the literature revealed that 592 polychaete species have been recorded from this area. Five of the species identified are reported for the first time from the Mediterranean and the Black Sea, 38 species are new records for the Eastern Mediterranean and 55 are new elements for the Aegean polychaete fauna. Information on the habitat and the geographic distribution of the species newly reported from the Eastern Mediterranean is given. The checklist of the Aegean Polychaeta is presented. Although being located in the vicinity of the Eastern Mediterranean province and the Black Sea which are characterized as faunistically impoverished areas, the Aegean hosts a rich polychaete fauna, second in order in species diversity after the Western Mediterranean. The latter demonstrates the importance of this area in the understanding the patterns of polychaete distribution along the Mediterranean and the Black Sea. |
Plaiti, W; Akoumianaki, I; Eleftheriou, A Seasonal and bathymetric trends of the amphipod fauna in Heraklion Bay (Aegean Sea) Journal Article Polskie Archiwum Hydrobiologii, 47 , pp. 517 – 525, 2000. @article{plaiti_seasonal_2000, title = {Seasonal and bathymetric trends of the amphipod fauna in Heraklion Bay (Aegean Sea)}, author = {W Plaiti and I Akoumianaki and A Eleftheriou}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034430130&partnerID=40&md5=6f5edad5cfd6e473fac2226dc4071988}, year = {2000}, date = {2000-01-01}, journal = {Polskie Archiwum Hydrobiologii}, volume = {47}, pages = {517 -- 525}, abstract = {The distribution of the benthic amphipod fauna in Heraklion Bay along a bathymetric gradient from 70 to 160 m was investigated during 1988-89 and 1991-92 by means of quantitative grab sampling. A total of 1456 individuals belonging to 38 species was found in 305 grab samples. Differences in the distribution of the dominant species as well in the structure of the amphipod community, as evidenced by cluster analysis, indicate the presence of distinct environmental conditions between the upper and lower circalittoral zone of Heraklion Bay. Temporal variability although evident does not clearly correspond to expected seasonal trends.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The distribution of the benthic amphipod fauna in Heraklion Bay along a bathymetric gradient from 70 to 160 m was investigated during 1988-89 and 1991-92 by means of quantitative grab sampling. A total of 1456 individuals belonging to 38 species was found in 305 grab samples. Differences in the distribution of the dominant species as well in the structure of the amphipod community, as evidenced by cluster analysis, indicate the presence of distinct environmental conditions between the upper and lower circalittoral zone of Heraklion Bay. Temporal variability although evident does not clearly correspond to expected seasonal trends. |
Pavlidis, M; Greenwood, L; Mourot, B; Kokkari, C; Menn, Le F; Divanach, P; Scott, A P General and Comparative Endocrinology, 118 , pp. 14 – 25, 2000. @article{pavlidis_seasonal_2000, title = {Seasonal variations and maturity stages in relation to differences in serum levels of gonadal steroids, vitellogenin, and thyroid hormones in the common dentex (Dentex dentex)}, author = {M Pavlidis and L Greenwood and B Mourot and C Kokkari and F Le Menn and P Divanach and A P Scott}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034006188&partnerID=40&md5=d5286b0db60ae52af7841e4963700b70}, year = {2000}, date = {2000-01-01}, journal = {General and Comparative Endocrinology}, volume = {118}, pages = {14 -- 25}, abstract = {Seasonal variations in serum concentrations of 17β-estradiol (E2), vitellogenin (Vg), testosterone (T), 11 ketotestosterone (11-KT), and thyroid hormones (T4, L-thyroxine; and T3, 3,5,3’-triiodo-L-thyronine) were investigated during the first, second, and third reproductive cycles in intensively reared populations of common dentex, Dentex dentex, and correlated with gonadal development and spawning. In females, there were baseline E2 values (<0.10 ng/ml) and negligible Vg concentrations during the postspawning and pregametogenesis period (June to December), and these increased thereafter to peak during the spawning period. Maximum T3 and T4 serum concentrations were found around spawning. There was a positive correlation during vitellogenesis and final maturation between Vg and T3 (r2 = 0.366). In addition, Vg and T3 concentrations were statistically higher in the stages of vitellogenesis and final maturation than at the other stages (P < 0.001). Minimum T3 and T4 concentrations (October) coincided with the decrease in water temperature and the associated decrease in the daily feeding rate and the specific growth rate. In males, as in females, seasonal changes in serum levels of T and 11-KT were well correlated with gonadal development. The presence of males in the stage of completed spermiogenesis in December coincided with the surge in both androgens and this increase lasted until the end of the spawning period. There were no significant differences in serum T3 and T4 levels among the maturity stages. The observed seasonal changes in serum gonadal steroids and Vg reflected the pattern of oocyte development and the spawning behavior of common dentex and were typical of the patterns described in most multiple spawners studied to date. Thyroid hormones may enhance early ovarian development and stimulate vitellogenesis in female dentex. (C) 2000 Academic Press.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Seasonal variations in serum concentrations of 17β-estradiol (E2), vitellogenin (Vg), testosterone (T), 11 ketotestosterone (11-KT), and thyroid hormones (T4, L-thyroxine; and T3, 3,5,3’-triiodo-L-thyronine) were investigated during the first, second, and third reproductive cycles in intensively reared populations of common dentex, Dentex dentex, and correlated with gonadal development and spawning. In females, there were baseline E2 values (<0.10 ng/ml) and negligible Vg concentrations during the postspawning and pregametogenesis period (June to December), and these increased thereafter to peak during the spawning period. Maximum T3 and T4 serum concentrations were found around spawning. There was a positive correlation during vitellogenesis and final maturation between Vg and T3 (r2 = 0.366). In addition, Vg and T3 concentrations were statistically higher in the stages of vitellogenesis and final maturation than at the other stages (P < 0.001). Minimum T3 and T4 concentrations (October) coincided with the decrease in water temperature and the associated decrease in the daily feeding rate and the specific growth rate. In males, as in females, seasonal changes in serum levels of T and 11-KT were well correlated with gonadal development. The presence of males in the stage of completed spermiogenesis in December coincided with the surge in both androgens and this increase lasted until the end of the spawning period. There were no significant differences in serum T3 and T4 levels among the maturity stages. The observed seasonal changes in serum gonadal steroids and Vg reflected the pattern of oocyte development and the spawning behavior of common dentex and were typical of the patterns described in most multiple spawners studied to date. Thyroid hormones may enhance early ovarian development and stimulate vitellogenesis in female dentex. (C) 2000 Academic Press. |
Hawkins, A J S; Magoulas, A; Héral, M; Bougrier, S; Naciri-Graven, Y; Day, A J; Kotoulas, G Genetical Research, 76 (3), pp. 273–284, 2000, ISSN: 00166723. @article{hawkins_separate_2000, title = {Separate effects of triploidy, parentage and genomic diversity upon feeding behaviour, metabolic efficiency and net energy balance in the pacific oyster Crassostrea gigas}, author = {A J S Hawkins and A Magoulas and M Héral and S Bougrier and Y Naciri-Graven and A J Day and G Kotoulas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034521887&doi=10.1017%2fS0016672300004766&partnerID=40&md5=b1e5a6ea52adb13f396dcec5d01c96d9}, doi = {10.1017/S0016672300004766}, issn = {00166723}, year = {2000}, date = {2000-01-01}, journal = {Genetical Research}, volume = {76}, number = {3}, pages = {273--284}, abstract = {Triploid oysters were induced using cytochalasin B upon retention of either the first (meiosis I triploids) or the second (meiosis II triploids) polar body in embryos from a single cohort derived from mixed parentage. Allozyme and microsatellite assays enabled the confirmation of both parentage and triploidy status in each oyster. Comparison of meiosis I triploids, meiosis II triploids and diploid siblings established that improved physiological performance in triploids was associated with increased allelic variation, rather than with the quantitative dosage effects of ploidy status. An unidentified maternal influence also interacted with genotype. Among full sibs, allelic variation measured as multi-locus enzyme heterozygosity accounted for up to 42% of the variance in physiological performance; significant positive influences were identified upon feeding rate, absorption efficiency, net energy balance and growth efficiency (= net energy balance ÷ energy absorbed). Whilst allelic variation was greater in both meiosis I and meiosis II triploids than in diploid siblings, both allelic variation and net energy balance were highest in triploids induced at meiosis I. This suggests that it may be preferable to induce triploidy by blocking meiosis I, rather than meiosis II as has traditionally been undertaken during commercial breeding programmes.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Triploid oysters were induced using cytochalasin B upon retention of either the first (meiosis I triploids) or the second (meiosis II triploids) polar body in embryos from a single cohort derived from mixed parentage. Allozyme and microsatellite assays enabled the confirmation of both parentage and triploidy status in each oyster. Comparison of meiosis I triploids, meiosis II triploids and diploid siblings established that improved physiological performance in triploids was associated with increased allelic variation, rather than with the quantitative dosage effects of ploidy status. An unidentified maternal influence also interacted with genotype. Among full sibs, allelic variation measured as multi-locus enzyme heterozygosity accounted for up to 42% of the variance in physiological performance; significant positive influences were identified upon feeding rate, absorption efficiency, net energy balance and growth efficiency (= net energy balance ÷ energy absorbed). Whilst allelic variation was greater in both meiosis I and meiosis II triploids than in diploid siblings, both allelic variation and net energy balance were highest in triploids induced at meiosis I. This suggests that it may be preferable to induce triploidy by blocking meiosis I, rather than meiosis II as has traditionally been undertaken during commercial breeding programmes. |
Lampadariou, N; Karakassis, Ioannis; Chapdelaine, L; Wilkinson, M F; Dafnomili, E Spatial variability in water column and sediment chemistry in Heraklion harbour (Crete, E. Mediterranean Journal Article Fresenius Envir Bull, 9 , pp. 164–171, 2000. @article{lampadariou_spatial_2000, title = {Spatial variability in water column and sediment chemistry in Heraklion harbour (Crete, E. Mediterranean}, author = {N Lampadariou and Ioannis Karakassis and L Chapdelaine and M F Wilkinson and E Dafnomili}, year = {2000}, date = {2000-01-01}, journal = {Fresenius Envir Bull}, volume = {9}, pages = {164--171}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Athanassopoulou, F; Cotou, E; Watsos, I; Giagnisi, M Study of the bacteriostatic activity of an Artemia enrichment compound based on plant extracts from Angelica sp Journal Article Bulletin of the Hellenic Veterinary Medical Society, 51 , pp. 293–296, 2000. @article{athanassopoulou_study_2000, title = {Study of the bacteriostatic activity of an Artemia enrichment compound based on plant extracts from Angelica sp}, author = {F Athanassopoulou and E Cotou and I Watsos and M Giagnisi}, year = {2000}, date = {2000-01-01}, journal = {Bulletin of the Hellenic Veterinary Medical Society}, volume = {51}, pages = {293--296}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Triantafyllou, G; Petihakis, G; Dounas, C; Koutsoubas, D; Arvanitidis, C; Eleftheriou, A Temporal variations in benthic communities and their response to physicochemical forcing: A numerical approach Journal Article ICES Journal of Marine Science, 57 (5), pp. 1507–1516, 2000, ISSN: 10543139, (Publisher: Academic Press). @article{triantafyllou_temporal_2000, title = {Temporal variations in benthic communities and their response to physicochemical forcing: A numerical approach}, author = {G Triantafyllou and G Petihakis and C Dounas and D Koutsoubas and C Arvanitidis and A Eleftheriou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034492083&doi=10.1006%2fjmsc.2000.0923&partnerID=40&md5=37dbe9cbf95e8c4e2e62b7f6e7c163d2}, doi = {10.1006/jmsc.2000.0923}, issn = {10543139}, year = {2000}, date = {2000-01-01}, journal = {ICES Journal of Marine Science}, volume = {57}, number = {5}, pages = {1507--1516}, abstract = {A generic complex ecological model, the European Regional Seas Ecosystem Model (ERSEM), was applied to a shallow lagoon system in the Eastern Mediterranean. Model results depicting the seasonal variation of nutrients and Chl-α in the water column, as well as three benthic functional groups (suspended feeders, deposit feeders, and benthic carnivores), are validated with in situ data. The likely effect of a technical intervention (river input) increasing the freshwater nutrient inputs on ecosystem functioning is also investigated. Detailed annual carbon fluxes and benthic fauna biomasses are calculated, before and after the river input. The importance of external physical/chemical forcing on the pelagic system and its subsequent effect on the benthic system are demonstrated. Model experiments indicate the shift of the ecosystem from nitrate limitation to predator control with external inputs. Model experiments also show a significant increase in the amount of carbon entering the benthic system through the activity of filter feeders when river inputs are implemented. © 2000 International Council for the Exploration of the Sea.}, note = {Publisher: Academic Press}, keywords = {}, pubstate = {published}, tppubtype = {article} } A generic complex ecological model, the European Regional Seas Ecosystem Model (ERSEM), was applied to a shallow lagoon system in the Eastern Mediterranean. Model results depicting the seasonal variation of nutrients and Chl-α in the water column, as well as three benthic functional groups (suspended feeders, deposit feeders, and benthic carnivores), are validated with in situ data. The likely effect of a technical intervention (river input) increasing the freshwater nutrient inputs on ecosystem functioning is also investigated. Detailed annual carbon fluxes and benthic fauna biomasses are calculated, before and after the river input. The importance of external physical/chemical forcing on the pelagic system and its subsequent effect on the benthic system are demonstrated. Model experiments indicate the shift of the ecosystem from nitrate limitation to predator control with external inputs. Model experiments also show a significant increase in the amount of carbon entering the benthic system through the activity of filter feeders when river inputs are implemented. © 2000 International Council for the Exploration of the Sea. |
Maurizi, A; Diaz, J P; Divanach, P; Papandroulakis, N; Connes, R The effect of glycerol dissolved in the rearing water on the transition to exotrophy in gilthead sea bream Sparus aurata larvae Journal Article Aquaculture, 189 , pp. 119 – 131, 2000. @article{maurizi_effect_2000, title = {The effect of glycerol dissolved in the rearing water on the transition to exotrophy in gilthead sea bream Sparus aurata larvae}, author = {A Maurizi and J P Diaz and P Divanach and N Papandroulakis and R Connes}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034715335&partnerID=40&md5=2ad2493b26d28332d0f11b019e7a552a}, year = {2000}, date = {2000-01-01}, journal = {Aquaculture}, volume = {189}, pages = {119 -- 131}, abstract = {The effects of glycerol added to seawater at different concentrations were tested in monocultures of Chlorella minutissima and Brachionus plicatilis in mixed cultures of these two species and on starved or fed Sparus aurata larvae. Glycerol enhanced the growth of Chlorella and seemed to indirectly benefit the rotifers that feed on it. Its beneficial effect on gilthead sea bream prelarvae and larvae resulted in glycogen accumulation in the liver and absence of signs of cholestasis-like pathology. This effect varied according to the glycerol concentration in the water, to its addition timing and to light and feeding conditions. The gluconeogenic origin of the hepatic glycogen reserves is discussed, together with the mediating role of the food chain in glycerol larval assimilation. (C) 2000 Elsevier Science B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The effects of glycerol added to seawater at different concentrations were tested in monocultures of Chlorella minutissima and Brachionus plicatilis in mixed cultures of these two species and on starved or fed Sparus aurata larvae. Glycerol enhanced the growth of Chlorella and seemed to indirectly benefit the rotifers that feed on it. Its beneficial effect on gilthead sea bream prelarvae and larvae resulted in glycogen accumulation in the liver and absence of signs of cholestasis-like pathology. This effect varied according to the glycerol concentration in the water, to its addition timing and to light and feeding conditions. The gluconeogenic origin of the hepatic glycogen reserves is discussed, together with the mediating role of the food chain in glycerol larval assimilation. (C) 2000 Elsevier Science B.V. |
McArthur, V E; Koutsoubas, D; Lampadariou, N; Dounas, C The meiofaunal community structure of a Mediterranean lagoon (Gialova lagoon, Ionian Sea) Journal Article Helgoland Marine Research, 54 (1), pp. 7–17, 2000, ISSN: 1438387X, (Publisher: Springer Verlag). @article{mcarthur_meiofaunal_2000, title = {The meiofaunal community structure of a Mediterranean lagoon (Gialova lagoon, Ionian Sea)}, author = {V E McArthur and D Koutsoubas and N Lampadariou and C Dounas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034125190&doi=10.1007%2fs101520050031&partnerID=40&md5=a39dd162e646b360cd77d24fb663534d}, doi = {10.1007/s101520050031}, issn = {1438387X}, year = {2000}, date = {2000-01-01}, journal = {Helgoland Marine Research}, volume = {54}, number = {1}, pages = {7--17}, abstract = {The higher taxonomic structure of a meiofaunal community was investigated in an Eastern Mediterranean lagoon (Gialova lagoon, Ionian Sea). Seven stations were sampled on a seasonal basis during 1995-1996. Stations within the lagoon were found to support a maximum of 14 meiofaunal taxa with densities ranging from 17 to over 2000 individuals per 10 cm2. Nematodes were not always the most abundant taxa, although they dominated about half of the total 28 samples. Uni- and multivariate analyses were employed to study the community structure. The distribution pattern of the meiofaunal community varied both across the lagoon and over the seasons. On the basis of the spatial differences a meiofaunal coenocline, correlated with the degree of isolation, was observed, composed of mainly two zones: one defined by the area close to the marine channel and the other the more isolated area in the inner lagoon. Meiofaunal distribution pattern was not clearly correlated to one environmental variable, but rather to many, and spatial and seasonal effects could be seen.}, note = {Publisher: Springer Verlag}, keywords = {}, pubstate = {published}, tppubtype = {article} } The higher taxonomic structure of a meiofaunal community was investigated in an Eastern Mediterranean lagoon (Gialova lagoon, Ionian Sea). Seven stations were sampled on a seasonal basis during 1995-1996. Stations within the lagoon were found to support a maximum of 14 meiofaunal taxa with densities ranging from 17 to over 2000 individuals per 10 cm2. Nematodes were not always the most abundant taxa, although they dominated about half of the total 28 samples. Uni- and multivariate analyses were employed to study the community structure. The distribution pattern of the meiofaunal community varied both across the lagoon and over the seasons. On the basis of the spatial differences a meiofaunal coenocline, correlated with the degree of isolation, was observed, composed of mainly two zones: one defined by the area close to the marine channel and the other the more isolated area in the inner lagoon. Meiofaunal distribution pattern was not clearly correlated to one environmental variable, but rather to many, and spatial and seasonal effects could be seen. |
Rigos, G; Kotzamanis, I; Gialamas, I; Nengas, I; Alexis, M Toxicity and digestibility of fumagillin DCH in gilthead sea bream, Sparus aurata L. Journal Article Journal of Fish Diseases, 23 , pp. 161 – 164, 2000. @article{rigos_toxicity_2000, title = {Toxicity and digestibility of fumagillin DCH in gilthead sea bream, Sparus aurata L.}, author = {G Rigos and I Kotzamanis and I Gialamas and I Nengas and M Alexis}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0033835878&partnerID=40&md5=fe8776f28328e1bb9903067f107d2210}, year = {2000}, date = {2000-01-01}, journal = {Journal of Fish Diseases}, volume = {23}, pages = {161 -- 164}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
P., Katharios; Κ., Kapata-Zoumbou; Iliopoulou-Georgudaki, J Toxicity of intraperitoneal administered ivermectin in sea bream, Sparus aurata. Inproceedings of Sciences, Hellenic Society Biological (Ed.): pp. 56–60, Chios, Greece, 2000. @inproceedings{katharios_toxicity_2000, title = {Toxicity of intraperitoneal administered ivermectin in sea bream, Sparus aurata.}, author = {Katharios P. and Kapata-Zoumbou Κ. and J Iliopoulou-Georgudaki}, editor = {Hellenic Society Biological of Sciences}, year = {2000}, date = {2000-01-01}, pages = {56--60}, address = {Chios, Greece}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Mylonas, C C; Zohar, Y Use of GnRHa-delivery systems for the control of reproduction in fish Journal Article Reviews in Fish Biology and Fisheries, 10 (4), pp. 463–491, 2000, ISSN: 09603166. @article{mylonas_use_2000, title = {Use of GnRHa-delivery systems for the control of reproduction in fish}, author = {C C Mylonas and Y Zohar}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034528406&doi=10.1023%2fA%3a1012279814708&partnerID=40&md5=93dc83bb17d6f49997555b7c15c0bd30}, doi = {10.1023/A:1012279814708}, issn = {09603166}, year = {2000}, date = {2000-01-01}, journal = {Reviews in Fish Biology and Fisheries}, volume = {10}, number = {4}, pages = {463--491}, abstract = {The most commonly observed reproductive dysfunctions in cultured fish are the unpredictability of final oocyte maturation (FOM) in females, and the diminished volume and quality of sperm in males. Gonadotropin-releasing hormone agonists (GnRHa) have been used extensively in order to stimulate the release of pituitary luteinizing hormone (LH) required to induce FOM, ovulation and spermiation. Because multiple hormonal treatments are often necessary for a successful response, fish must be monitored and handled extensively, which is labor intensive, stressful to the fish and can often result in broodstock mortalities. To ameliorate this problem, sustained-release delivery systems for GnRHa have been developed during the last two decades and have been increasingly applied in controlling reproduction of a variety of cultured fish. Solid implants of cholesterol or poly[ethylene-vinyl acetate], and biodegradable microspheres of poly[lactide-glycolide] or poly[fatty acid dimer-sebasic acid] release GnRHa for a period of time (from a few days to many weeks.) GnRHa-delivery systems do not cause desensitization of the pituitary gonadotrophs in fish, and by stimulating a sustained elevation of plasma LH they induce the natural progression of plasma steroid increases associated with FOM and spermiation. This method has been used with very encouraging results in females of more than 40 cultured species and has been effective in inducing FOM, ovulation or spawning in fish with synchronous, group-synchronous and asynchronous ovarian development. In males, GnRHa-delivery systems have been tested in more than 20 species, producing significant increases in milt production for up to 5 weeks. Future research should focus on the optimization of this technology in terms of (a) using the most potent GnRHa, (b) identifying the most appropriate GnRHa release kinetics according to the reproductive biology of different species, and (c) determining minimum effective doses. Developments in these areas will greatly enhance the effectiveness and efficiency of GnRHa-delivery systems, while at the same time reducing their cost thus making them more affordable to the aquaculture industry.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The most commonly observed reproductive dysfunctions in cultured fish are the unpredictability of final oocyte maturation (FOM) in females, and the diminished volume and quality of sperm in males. Gonadotropin-releasing hormone agonists (GnRHa) have been used extensively in order to stimulate the release of pituitary luteinizing hormone (LH) required to induce FOM, ovulation and spermiation. Because multiple hormonal treatments are often necessary for a successful response, fish must be monitored and handled extensively, which is labor intensive, stressful to the fish and can often result in broodstock mortalities. To ameliorate this problem, sustained-release delivery systems for GnRHa have been developed during the last two decades and have been increasingly applied in controlling reproduction of a variety of cultured fish. Solid implants of cholesterol or poly[ethylene-vinyl acetate], and biodegradable microspheres of poly[lactide-glycolide] or poly[fatty acid dimer-sebasic acid] release GnRHa for a period of time (from a few days to many weeks.) GnRHa-delivery systems do not cause desensitization of the pituitary gonadotrophs in fish, and by stimulating a sustained elevation of plasma LH they induce the natural progression of plasma steroid increases associated with FOM and spermiation. This method has been used with very encouraging results in females of more than 40 cultured species and has been effective in inducing FOM, ovulation or spawning in fish with synchronous, group-synchronous and asynchronous ovarian development. In males, GnRHa-delivery systems have been tested in more than 20 species, producing significant increases in milt production for up to 5 weeks. Future research should focus on the optimization of this technology in terms of (a) using the most potent GnRHa, (b) identifying the most appropriate GnRHa release kinetics according to the reproductive biology of different species, and (c) determining minimum effective doses. Developments in these areas will greatly enhance the effectiveness and efficiency of GnRHa-delivery systems, while at the same time reducing their cost thus making them more affordable to the aquaculture industry. |
1999 |
Asplin, Lars; Salvanes, Anne Gro Vea; Kristoffersen, Jon Bent Nonlocal wind-driven fjord-coast advection and its potential effect on plankton and fish recruitment Journal Article Fisheries Oceanography, 8 (4), pp. 255–263, 1999, ISSN: 1054-6006, 1365-2419. @article{asplin_nonlocal_1999, title = {Nonlocal wind-driven fjord-coast advection and its potential effect on plankton and fish recruitment}, author = {Lars Asplin and Anne Gro Vea Salvanes and Jon Bent Kristoffersen}, url = {http://doi.wiley.com/10.1046/j.1365-2419.1999.00109.x}, doi = {10.1046/j.1365-2419.1999.00109.x}, issn = {1054-6006, 1365-2419}, year = {1999}, date = {1999-12-01}, urldate = {2020-08-18}, journal = {Fisheries Oceanography}, volume = {8}, number = {4}, pages = {255--263}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Koumoundouros, G; Divanach, P; Kentouri, M Ontogeny and allometric plasticity of Dentex dentex (Osteichthyes: Sparidae) in rearing conditions Journal Article Marine Biology, 135 (3), pp. 561–572, 1999, ISSN: 0025-3162, 1432-1793. @article{koumoundouros_ontogeny_1999, title = {Ontogeny and allometric plasticity of Dentex dentex (Osteichthyes: Sparidae) in rearing conditions}, author = {G Koumoundouros and P Divanach and M Kentouri}, url = {http://link.springer.com/10.1007/s002270050657}, doi = {10.1007/s002270050657}, issn = {0025-3162, 1432-1793}, year = {1999}, date = {1999-12-01}, urldate = {2020-08-18}, journal = {Marine Biology}, volume = {135}, number = {3}, pages = {561--572}, abstract = {The ontogeny of Dentex dentex (Linnaeus, 1758) was studied comparatively under extensive and semi-extensive rearing conditions. The exotrophic phase was divided into three distinct developmental stages (a) larval, from feeding onset up to 10 mm total length (TL); (b) metamorphosis, from 10 mm TL up to 24 mm TL; and (c) further juveniles. The majority of all ontogenic changes was expressed during the larval stage and integrated during the metamorphosis stage. The juvenile stage was characterized by developmental stability. The normal development of D. dentex was seriously affected by the rearing conditions applied during the early exotrophic phase. The two differently reared populations followed similar developmental patterns of general morphology, pigmentation and behaviour, but they presented high developmental plasticity where allometric growth was concerned. Rearing conditions influenced both the boundaries of the sequential stages (in 50% of the morphometric ratios) and the allometry coefficient of each morphometric character in the various developmental stages (in 80% of the characters). The results are discussed with respect to the ontogeny of the functional morphology under both ecological and aquaculture considerations.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The ontogeny of Dentex dentex (Linnaeus, 1758) was studied comparatively under extensive and semi-extensive rearing conditions. The exotrophic phase was divided into three distinct developmental stages (a) larval, from feeding onset up to 10 mm total length (TL); (b) metamorphosis, from 10 mm TL up to 24 mm TL; and (c) further juveniles. The majority of all ontogenic changes was expressed during the larval stage and integrated during the metamorphosis stage. The juvenile stage was characterized by developmental stability. The normal development of D. dentex was seriously affected by the rearing conditions applied during the early exotrophic phase. The two differently reared populations followed similar developmental patterns of general morphology, pigmentation and behaviour, but they presented high developmental plasticity where allometric growth was concerned. Rearing conditions influenced both the boundaries of the sequential stages (in 50% of the morphometric ratios) and the allometry coefficient of each morphometric character in the various developmental stages (in 80% of the characters). The results are discussed with respect to the ontogeny of the functional morphology under both ecological and aquaculture considerations. |
Kristoffersen, JB; Salvanes, AGV Growth, life history and population genetics of Benthosema glacial in fjordic and oceanic environments Inproceedings Kristoffersen, JB (Ed.): Gilleleje, Denmark, 1999. @inproceedings{kristoffersen_growth_1999, title = {Growth, life history and population genetics of Benthosema glacial in fjordic and oceanic environments}, author = {JB Kristoffersen and AGV Salvanes}, editor = {JB Kristoffersen}, year = {1999}, date = {1999-11-01}, address = {Gilleleje, Denmark}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Nengas, Ioannis; Alexis, Maria N; Davies, Simon J High inclusion levels of poultry meals and related byproducts in diets for gilthead seabream Sparus aurata L. Journal Article Aquaculture, 179 (1-4), pp. 13–23, 1999, ISSN: 00448486. @article{nengas_high_1999, title = {High inclusion levels of poultry meals and related byproducts in diets for gilthead seabream Sparus aurata L.}, author = {Ioannis Nengas and Maria N Alexis and Simon J Davies}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848699001489}, doi = {10.1016/S0044-8486(99)00148-9}, issn = {00448486}, year = {1999}, date = {1999-09-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {179}, number = {1-4}, pages = {13--23}, abstract = {The feasibility of replacing fish meal protein at high levels of 75 and 100% with a high quality poultry meat meal was assessed in diets for gilthead seabream. A combined mixture of poultry meat meal and feather meal was also tested at high inclusion levels of 75 and 100%. Finally, two lower grade poultry by-product meals produced for the Greek industry were tested at various levels for comparison. The diets were isocaloric containing 18 MJ/kg of gross energy, isonitrogenous (CP: 45%) and had a lipid content of 13% on an as fed basis. The experiment was carried out in a semiclosed rearing system and its duration was 84 days. The groups of fish fed 75 and 100% poultry meat meal showed a slight reduction in growth parameters compared to fish fed the control diet containing white fish meal but was not statistically significant (P textless 0.05). Similar results were obtained for the fish fed the poultry and feather mixture. Feed efficiency, protein and energy utilisation followed the same trends. One of the locally produced meals at substitution levels of up to 50% produced no significant reduction in growth of seabream (P textless 0.05). At the level of 75% of the protein, however, the material caused a severe decrease in growth performance, feed efficiency, protein efficiency ratio and apparent net protein utilisation. Inferior quality was also demonstrated by the other poultry meal available on the Greek market. Protein digestibility coefficients were measured for all diets and essential amino acid indices and chemical score values were calculated.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The feasibility of replacing fish meal protein at high levels of 75 and 100% with a high quality poultry meat meal was assessed in diets for gilthead seabream. A combined mixture of poultry meat meal and feather meal was also tested at high inclusion levels of 75 and 100%. Finally, two lower grade poultry by-product meals produced for the Greek industry were tested at various levels for comparison. The diets were isocaloric containing 18 MJ/kg of gross energy, isonitrogenous (CP: 45%) and had a lipid content of 13% on an as fed basis. The experiment was carried out in a semiclosed rearing system and its duration was 84 days. The groups of fish fed 75 and 100% poultry meat meal showed a slight reduction in growth parameters compared to fish fed the control diet containing white fish meal but was not statistically significant (P textless 0.05). Similar results were obtained for the fish fed the poultry and feather mixture. Feed efficiency, protein and energy utilisation followed the same trends. One of the locally produced meals at substitution levels of up to 50% produced no significant reduction in growth of seabream (P textless 0.05). At the level of 75% of the protein, however, the material caused a severe decrease in growth performance, feed efficiency, protein efficiency ratio and apparent net protein utilisation. Inferior quality was also demonstrated by the other poultry meal available on the Greek market. Protein digestibility coefficients were measured for all diets and essential amino acid indices and chemical score values were calculated. |
Alexis, Maria N; Nengas, Ioannis; Fountoulaki, Eleni; Papoutsi, Eleni; Andriopoulou, Argiro; Koutsodimou, Maria; Gaubaudan, J Aquaculture, 179 (1-4), pp. 447–456, 1999, ISSN: 00448486. @article{alexis_tissue_1999, title = {Tissue ascorbic acid levels in European sea bass (Dicentrarchus labrax) and gilthead sea bream (Sparus aurata L.) fingerlings fed diets containing different forms of ascorbic acid}, author = {Maria N Alexis and Ioannis Nengas and Eleni Fountoulaki and Eleni Papoutsi and Argiro Andriopoulou and Maria Koutsodimou and J Gaubaudan}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848699001787}, doi = {10.1016/S0044-8486(99)00178-7}, issn = {00448486}, year = {1999}, date = {1999-09-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {179}, number = {1-4}, pages = {447--456}, abstract = {Sea bass and sea bream fingerlings of 3-4 g initial weight were fed four diets supplemented with 250 mg/kg of ascorbic acid (AA) supplied as the fat coated form or different phosphate ester forms. An ascorbate free diet was also fed. Before the start of the experiment, fish were fed an ascorbate free diet in order to deplete their ascorbate stores. The same diet formulation was used in all treatments containing fish meal LT, soybean meal and wheat flour as the main ingredients. Feeding levels were the same for all groups of fish and were close to satiation. The results obtained for fish growth and feed efficiency did not differ significantly among groups of fish for both species studied. Muscle, liver and kidney were analysed for AA content after frequent sampling of fish. Tissue ascorbate levels increased after feeding the ascorbate containing diets, the rate of increase was although higher for sea bream than for sea bass. Ascorbate levels in groups fed the fat coated diet were lower than those fed the diets containing phosphate esters, probably due to a higher destruction of this form during feed preparation and storage. AA levels in the tissues studied were different with muscle having the lowest and kidney the highest value. Ascorbate levels in fish fed the ascorbate free diet decreased significantly. No growth reduction or deficiency signs were observed.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Sea bass and sea bream fingerlings of 3-4 g initial weight were fed four diets supplemented with 250 mg/kg of ascorbic acid (AA) supplied as the fat coated form or different phosphate ester forms. An ascorbate free diet was also fed. Before the start of the experiment, fish were fed an ascorbate free diet in order to deplete their ascorbate stores. The same diet formulation was used in all treatments containing fish meal LT, soybean meal and wheat flour as the main ingredients. Feeding levels were the same for all groups of fish and were close to satiation. The results obtained for fish growth and feed efficiency did not differ significantly among groups of fish for both species studied. Muscle, liver and kidney were analysed for AA content after frequent sampling of fish. Tissue ascorbate levels increased after feeding the ascorbate containing diets, the rate of increase was although higher for sea bream than for sea bass. Ascorbate levels in groups fed the fat coated diet were lower than those fed the diets containing phosphate esters, probably due to a higher destruction of this form during feed preparation and storage. AA levels in the tissues studied were different with muscle having the lowest and kidney the highest value. Ascorbate levels in fish fed the ascorbate free diet decreased significantly. No growth reduction or deficiency signs were observed. |
Rufingier, Catherine; Pasteur, Nicole; Lagnel, Jacques; Martin, Christian; Navajas, Maria Mechanisms of insecticide resistance in the aphid Nasonovia ribisnigri (Mosley) (Homoptera: Aphididae) from France Journal Article Insect Biochemistry and Molecular Biology, 29 (4), pp. 385–391, 1999, ISSN: 09651748. @article{rufingier_mechanisms_1999, title = {Mechanisms of insecticide resistance in the aphid Nasonovia ribisnigri (Mosley) (Homoptera: Aphididae) from France}, author = {Catherine Rufingier and Nicole Pasteur and Jacques Lagnel and Christian Martin and Maria Navajas}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0965174899000144}, doi = {10.1016/S0965-1748(99)00014-4}, issn = {09651748}, year = {1999}, date = {1999-04-01}, urldate = {2020-08-18}, journal = {Insect Biochemistry and Molecular Biology}, volume = {29}, number = {4}, pages = {385--391}, abstract = {Nasonovia ribisnigri, a main pest of salad crops, has developed resistance to Various insecticides in southern France, including the carbamate pirimicarb and the cyclodiene endosulfan, two insecticides widely used to control this aphid. Here we have investigated the mechanisms of resistance to these two insecticides by studying cross-resistance, synergism, activity of detoxifying enzymes, and possible modifications of the target proteins. Resistance to pirimicarb was shown to be mainly due to a decreased sensitivity of the target acetylcholinesterase; this modification confered also, resistance to propoxur but not to methomyl and the two tested organophosphates (acephate and paraoxon). Endosulfan resistance was associated with a moderate level of resistance to dieldrin, and resistance to both insecticides was due, in part, to increased detoxification by glutathione S-transferases (GST). The endosulfan resistant strain displayed the same amino acid at position 302 of the Rdl gene (GABA receptor) as susceptible aphids (e.g. Ala), indicating that the Ala to Ser (or to Gly) mutation observed among dieldrin resistant strains of other insect species was not present. (C) 1999 Elsevier Science Ltd. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Nasonovia ribisnigri, a main pest of salad crops, has developed resistance to Various insecticides in southern France, including the carbamate pirimicarb and the cyclodiene endosulfan, two insecticides widely used to control this aphid. Here we have investigated the mechanisms of resistance to these two insecticides by studying cross-resistance, synergism, activity of detoxifying enzymes, and possible modifications of the target proteins. Resistance to pirimicarb was shown to be mainly due to a decreased sensitivity of the target acetylcholinesterase; this modification confered also, resistance to propoxur but not to methomyl and the two tested organophosphates (acephate and paraoxon). Endosulfan resistance was associated with a moderate level of resistance to dieldrin, and resistance to both insecticides was due, in part, to increased detoxification by glutathione S-transferases (GST). The endosulfan resistant strain displayed the same amino acid at position 302 of the Rdl gene (GABA receptor) as susceptible aphids (e.g. Ala), indicating that the Ala to Ser (or to Gly) mutation observed among dieldrin resistant strains of other insect species was not present. (C) 1999 Elsevier Science Ltd. All rights reserved. |
Makridis, Pavlos; Olsen, Yngvar Protein depletion of the rotifer Brachionus plicatilis during starvation Journal Article Aquaculture, 174 (3-4), pp. 343–353, 1999, ISSN: 00448486. @article{makridis_protein_1999, title = {Protein depletion of the rotifer Brachionus plicatilis during starvation}, author = {Pavlos Makridis and Yngvar Olsen}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848699000204}, doi = {10.1016/S0044-8486(99)00020-4}, issn = {00448486}, year = {1999}, date = {1999-04-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {174}, number = {3-4}, pages = {343--353}, abstract = {During the cultivation of marine fish larvae, unconsumed rotifers (Brachionus plicatilis) may reside in the tanks for several days and their nutritional value may become severely reduced. In order to quantify this process, body biomass and protein content per individual rotifer was measured during starvation at different temperatures (4-28°C) for a period of 5 to 7 days. Both well-fed rotifers and poorly-fed rotifers were used, in order to examine the effect of the nutritional status at the start of the experiment on the loss rates of biomass and protein. At high temperatures the carbon, nitrogen and amino acid content decreased exponentially with time. A large part of body biomass (40-50%) was lost during the first 4 days of starvation at 18-20°C. The rate of decrease was positively related to the temperature. Poorly-fed rotifers were more sensitive to starvation than well-fed rotifers, as their nitrogen content decreased at a higher rate. Starvation of rotifers may be prevented by addition of microalgae in concentrations of 1-2 mg C l-1. At 4-5°C, the carbon, nitrogen and amino acid content remained relatively stable. Rotifers may thus be stored for < 5 days at 4-5°C without a significant loss in their nutritional value.}, keywords = {}, pubstate = {published}, tppubtype = {article} } During the cultivation of marine fish larvae, unconsumed rotifers (Brachionus plicatilis) may reside in the tanks for several days and their nutritional value may become severely reduced. In order to quantify this process, body biomass and protein content per individual rotifer was measured during starvation at different temperatures (4-28°C) for a period of 5 to 7 days. Both well-fed rotifers and poorly-fed rotifers were used, in order to examine the effect of the nutritional status at the start of the experiment on the loss rates of biomass and protein. At high temperatures the carbon, nitrogen and amino acid content decreased exponentially with time. A large part of body biomass (40-50%) was lost during the first 4 days of starvation at 18-20°C. The rate of decrease was positively related to the temperature. Poorly-fed rotifers were more sensitive to starvation than well-fed rotifers, as their nitrogen content decreased at a higher rate. Starvation of rotifers may be prevented by addition of microalgae in concentrations of 1-2 mg C l-1. At 4-5°C, the carbon, nitrogen and amino acid content remained relatively stable. Rotifers may thus be stored for < 5 days at 4-5°C without a significant loss in their nutritional value. |
Navajas, M; Gutierrez, J; Lagnel, J; Fauvel, G; Gotoh, T Entomologia Experimentalis Et Applicata, 90 (2), pp. 113–122, 1999, (ISBN: 0013-8703). @article{navajas_dna_1999, title = {DNA sequences and cross-breeding experiments in the hawthorn spider mite Amphitetranychus viennensis reveal high genetic differentiation between Japanese and French populations}, author = {M Navajas and J Gutierrez and J Lagnel and G Fauvel and T Gotoh}, url = {<Go to ISI>://000080275700001}, year = {1999}, date = {1999-02-01}, journal = {Entomologia Experimentalis Et Applicata}, volume = {90}, number = {2}, pages = {113--122}, abstract = {Sequence variation of the complete second internal transcribed spacer (ITS2, 445 bp) of nuclear ribosomal DNA and part of the mitochondrial cytochrome oxidase I gene (COI, 350 bp) was examined in Amphitetranychus viennensis (Zacher) mites (Acari:Tetranychidae) from four French and four Japanese locations. Sequence analysis consistently revealed the separation of the samples in two major groups: French mites differed from Japanese by 3.8-4.1% of the nucleotide divergence in COI sequences. These two groups also displayed distinct ITS2 consensus sequences (2.1% nucleotide divergence). A few variations, not affecting the diagnostic sites around the consensus sequence, were revealed among cloned copies of the same individual. Reciprocal crosses and backcrosses between one French and two Japanese populations disclosed strong reproductive incompatibility. However, fertile hybrid females were obtained, indicating the conspecificity of the tested mites. Despite the presence of Wolbachia in the French strain, but not in the Japanese ones, our crosses did not display the unidirectional incompatibility typically produced by this microorganism, but rather a bidirectional - although asymmetrical - incompatibility pattern. The post-zygotic incompatibilities in A. viennensis cannot be explained by the presence of Wolbachia but to some extent by mite genome divergence resulting from limited gene exchange between allopatric populations. Experiments of Wolbachia elimination by antibiotic treatment and subsequent crosses with cured strains are still needed to fully understand the reproductive incompatibility patterns in this mite species.}, note = {ISBN: 0013-8703}, keywords = {}, pubstate = {published}, tppubtype = {article} } Sequence variation of the complete second internal transcribed spacer (ITS2, 445 bp) of nuclear ribosomal DNA and part of the mitochondrial cytochrome oxidase I gene (COI, 350 bp) was examined in Amphitetranychus viennensis (Zacher) mites (Acari:Tetranychidae) from four French and four Japanese locations. Sequence analysis consistently revealed the separation of the samples in two major groups: French mites differed from Japanese by 3.8-4.1% of the nucleotide divergence in COI sequences. These two groups also displayed distinct ITS2 consensus sequences (2.1% nucleotide divergence). A few variations, not affecting the diagnostic sites around the consensus sequence, were revealed among cloned copies of the same individual. Reciprocal crosses and backcrosses between one French and two Japanese populations disclosed strong reproductive incompatibility. However, fertile hybrid females were obtained, indicating the conspecificity of the tested mites. Despite the presence of Wolbachia in the French strain, but not in the Japanese ones, our crosses did not display the unidirectional incompatibility typically produced by this microorganism, but rather a bidirectional - although asymmetrical - incompatibility pattern. The post-zygotic incompatibilities in A. viennensis cannot be explained by the presence of Wolbachia but to some extent by mite genome divergence resulting from limited gene exchange between allopatric populations. Experiments of Wolbachia elimination by antibiotic treatment and subsequent crosses with cured strains are still needed to fully understand the reproductive incompatibility patterns in this mite species. |
Koumoundouros, G; Divanach, P; Kentouri, M Osteological development of the vertebral column and of the caudal complex in Dentex dentex Journal Article Journal of Fish Biology, 54 (2), pp. 424–436, 1999, ISSN: 0022-1112, 1095-8649. @article{koumoundouros_osteological_1999, title = {Osteological development of the vertebral column and of the caudal complex in Dentex dentex}, author = {G Koumoundouros and P Divanach and M Kentouri}, url = {http://doi.wiley.com/10.1111/j.1095-8649.1999.tb00841.x}, doi = {10.1111/j.1095-8649.1999.tb00841.x}, issn = {0022-1112, 1095-8649}, year = {1999}, date = {1999-02-01}, urldate = {2020-08-18}, journal = {Journal of Fish Biology}, volume = {54}, number = {2}, pages = {424--436}, abstract = {Osteological development of the vertebral column and caudal complex in common dentex was described under extensive larval rearing conditions. Generally, the cartilaginous bones developed prior to the membranous bones. The development of the axial skeleton began with the formation of the hypural 1, the neural arches 2 and 3, as well as the haemal arches 1-8 at 4.8, 4.9 and 5.0 mm total length (L(T), measured in vivo), respectively. By 7.5 mm L(T), all the cartilaginous elements were formed, except for the ventral ribs, which formed between the range of 8.4-18.0 mm L(T) The caudal lepidotrichia were the first membranous bones to appear (5.3 mm L(T)) and attain their full meristic count (7.4 mm L(T)), followed by the vertebral centra, which formed between 6.6 and 9.7 mm L(T). By 25.0 mm L(T), all the elements were fully ossified except for the ventral ribs. The developmental direction and order of all the elements were studied with respect to their formation and ossification. The results were discussed in the contexts of ichthyoplankton, ecology and aquaculture. Compared with other Sparidae species, common dentex followed a pattern of relatively rapid rate of osteological development.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Osteological development of the vertebral column and caudal complex in common dentex was described under extensive larval rearing conditions. Generally, the cartilaginous bones developed prior to the membranous bones. The development of the axial skeleton began with the formation of the hypural 1, the neural arches 2 and 3, as well as the haemal arches 1-8 at 4.8, 4.9 and 5.0 mm total length (L(T), measured in vivo), respectively. By 7.5 mm L(T), all the cartilaginous elements were formed, except for the ventral ribs, which formed between the range of 8.4-18.0 mm L(T) The caudal lepidotrichia were the first membranous bones to appear (5.3 mm L(T)) and attain their full meristic count (7.4 mm L(T)), followed by the vertebral centra, which formed between 6.6 and 9.7 mm L(T). By 25.0 mm L(T), all the elements were fully ossified except for the ventral ribs. The developmental direction and order of all the elements were studied with respect to their formation and ossification. The results were discussed in the contexts of ichthyoplankton, ecology and aquaculture. Compared with other Sparidae species, common dentex followed a pattern of relatively rapid rate of osteological development. |
Arvanitidis, C; Koutsoubas, D; Dounas, C; Eleftheriou, A Annelid fauna of a Mediterranean lagoon (Gialova Lagoon, south-west Greece): Community structure in a severely fluctuating environment Journal Article Journal of the Marine Biological Association of the United Kingdom, 79 (5), pp. 849–856, 1999, ISSN: 00253154. @article{arvanitidis_annelid_1999, title = {Annelid fauna of a Mediterranean lagoon (Gialova Lagoon, south-west Greece): Community structure in a severely fluctuating environment}, author = {C Arvanitidis and D Koutsoubas and C Dounas and A Eleftheriou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032745818&doi=10.1017%2fS0025315499001010&partnerID=40&md5=b5ac264673a03e1f15a7a88f2be04fd2}, doi = {10.1017/S0025315499001010}, issn = {00253154}, year = {1999}, date = {1999-01-01}, journal = {Journal of the Marine Biological Association of the United Kingdom}, volume = {79}, number = {5}, pages = {849--856}, abstract = {The annelid community of a shallow Mediterranean lagoon (Gialova Lagoon, Ionian Sea) was studied on a seasonal basis. Out of the 39 species identified eight are reported for the first time from the central Mediterranean. The dominant species were the polychaetes Capitella capitata, Neodexiospira pseudocorrugata, Malacoceros fuliginosus, Perinereis cultrifera, Hediste diversicolor, Heteromastus filiformis and the oligochaete Limnodriloides maslinicensis. Uni- and multivariate methods were employed to study the community structure. The coenocline observed is strongly related to the degree of isolation; its main features (i.e. number of species, density, geometric abundance and size-classes) in space and time are presented and discussed in detail. The distribution pattern of the annelid community has been found to be governed by a different set of environmental factors in each season. Although this narrow lagoonal habitat suffers severe dystrophic episodes it manages to recover, demonstrating a seasonal community pattern.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The annelid community of a shallow Mediterranean lagoon (Gialova Lagoon, Ionian Sea) was studied on a seasonal basis. Out of the 39 species identified eight are reported for the first time from the central Mediterranean. The dominant species were the polychaetes Capitella capitata, Neodexiospira pseudocorrugata, Malacoceros fuliginosus, Perinereis cultrifera, Hediste diversicolor, Heteromastus filiformis and the oligochaete Limnodriloides maslinicensis. Uni- and multivariate methods were employed to study the community structure. The coenocline observed is strongly related to the degree of isolation; its main features (i.e. number of species, density, geometric abundance and size-classes) in space and time are presented and discussed in detail. The distribution pattern of the annelid community has been found to be governed by a different set of environmental factors in each season. Although this narrow lagoonal habitat suffers severe dystrophic episodes it manages to recover, demonstrating a seasonal community pattern. |
Karakassis, I; Hatziyanni, E; Tsapakis, M; Plaiti, W Benthic recovery following cessation of fish farming:a series of successes and catastrophes Journal Article Marine Ecology Progress Series, 184 , pp. 205–218, 1999, ISSN: 0171-8630, 1616-1599. @article{karakassis_benthic_1999, title = {Benthic recovery following cessation of fish farming:a series of successes and catastrophes}, author = {I Karakassis and E Hatziyanni and M Tsapakis and W Plaiti}, url = {http://www.int-res.com/abstracts/meps/v184/p205-218/}, doi = {10.3354/meps184205}, issn = {0171-8630, 1616-1599}, year = {1999}, date = {1999-01-01}, urldate = {2020-08-17}, journal = {Marine Ecology Progress Series}, volume = {184}, pages = {205--218}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Batargias, C; Dermitzakis, E; Magoulas, A; Zouros, E Characterization of six polymorphic microsatellite markers in gilthead sea bream, Sparus aurata (Linnaeus 1758) Journal Article Molecular Ecology, 8 , pp. 897–898, 1999. @article{batargias_characterization_1999, title = {Characterization of six polymorphic microsatellite markers in gilthead sea bream, Sparus aurata (Linnaeus 1758)}, author = {C Batargias and E Dermitzakis and A Magoulas and E Zouros}, year = {1999}, date = {1999-01-01}, journal = {Molecular Ecology}, volume = {8}, pages = {897--898}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Triantafyllidis, G; Pantazis, G; Xouri, G; Papadopoulou, K -N; Chatzinikolaou, Eva; Economou, A N; Tafas, T Cryopreservation of microalgae and toxicity of cryoprotectants for aquaculture Inproceedings Proceedings 21st Panhellenic Meeting of Hellenic Society of Biological Sciences, Syros, Greece, 1999, (Publication Title: Proceedings 21st Panhellenic Meeting of Hellenic Society of Biological Sciences). @inproceedings{triantafyllidis_cryopreservation_1999, title = {Cryopreservation of microalgae and toxicity of cryoprotectants for aquaculture}, author = {G Triantafyllidis and G Pantazis and G Xouri and K -N Papadopoulou and Eva Chatzinikolaou and A N Economou and T Tafas}, year = {1999}, date = {1999-01-01}, booktitle = {Proceedings 21st Panhellenic Meeting of Hellenic Society of Biological Sciences}, address = {Syros, Greece}, note = {Publication Title: Proceedings 21st Panhellenic Meeting of Hellenic Society of Biological Sciences}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Pavlidis, M; Paspatis, M; Koistinen, M; Paavola, T; Divanach, P; Kentouri, M Diel rhythms of serum metabolites and thyroid hormones in red porgy held in different photoperiod regimes Journal Article Aquaculture International, 7 , pp. 29 – 44, 1999. @article{pavlidis_diel_1999, title = {Diel rhythms of serum metabolites and thyroid hormones in red porgy held in different photoperiod regimes}, author = {M Pavlidis and M Paspatis and M Koistinen and T Paavola and P Divanach and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0032712520&partnerID=40&md5=1435b4ae75810998a0eeb86912538d21}, year = {1999}, date = {1999-01-01}, journal = {Aquaculture International}, volume = {7}, pages = {29 -- 44}, abstract = {Diel rhythms in serum glucose, lactate, cholesterol, triglycerides and thyroid hormones were studied in red porgy, Pagrus pagrus, held under different photoperiod regimes (constant darkness - DD, 8L:16D,12L:12D), at a constant temperature (17.1-18.7 °C) and fed with commercial pellets, by means of a self-feeder. A clear diurnal rhythm in feeding activity, regardless of the photoperiod regime was demonstrated. All serum components showed significant diel rhythms, although they were not necessarily consistent or circadian in periodicity. As well as this, differences in the average values among the varying treatments were observed. Fish held under the 12L:12D protocol displayed significantly higher average T4, T3 and lactate levels during the day rather than at night. Maximum glucose values occurred 8-12 h after dawn and maximum lactate concentrations at 06:00 or 14:00 h. Diel variations in cholesterol were evident only in the DD group with peak values inversely correlated with the rhythm of food intake. Triglycerides displayed a similar pattern of changes. Significant diel fluctuations in T4 serum levels were observed only in fish exposed to the 12L:12D protocol, with peak values at dawn. A clear diurnal peak (10:00h) in T3 concentrations was observed in fish subjected to the 12L:12D regime, while fish exposed to the 8L:16D protocol showed two peaks: one in the photophase (10:00 h) and another in the scotophase (02:00 h). The light-dark alternation and the general activity rhythm of fish seem to be the main synchronizers of the diel rhythms observed in this study.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Diel rhythms in serum glucose, lactate, cholesterol, triglycerides and thyroid hormones were studied in red porgy, Pagrus pagrus, held under different photoperiod regimes (constant darkness - DD, 8L:16D,12L:12D), at a constant temperature (17.1-18.7 °C) and fed with commercial pellets, by means of a self-feeder. A clear diurnal rhythm in feeding activity, regardless of the photoperiod regime was demonstrated. All serum components showed significant diel rhythms, although they were not necessarily consistent or circadian in periodicity. As well as this, differences in the average values among the varying treatments were observed. Fish held under the 12L:12D protocol displayed significantly higher average T4, T3 and lactate levels during the day rather than at night. Maximum glucose values occurred 8-12 h after dawn and maximum lactate concentrations at 06:00 or 14:00 h. Diel variations in cholesterol were evident only in the DD group with peak values inversely correlated with the rhythm of food intake. Triglycerides displayed a similar pattern of changes. Significant diel fluctuations in T4 serum levels were observed only in fish exposed to the 12L:12D protocol, with peak values at dawn. A clear diurnal peak (10:00h) in T3 concentrations was observed in fish subjected to the 12L:12D regime, while fish exposed to the 8L:16D protocol showed two peaks: one in the photophase (10:00 h) and another in the scotophase (02:00 h). The light-dark alternation and the general activity rhythm of fish seem to be the main synchronizers of the diel rhythms observed in this study. |
Makridis, P; Vadstein, O Food size selectivity of Artemia franciscana at three developmental stages Journal Article Journal of Plankton Research, 21 (11), pp. 2191–2201, 1999, ISSN: 14643774. @article{makridis_food_1999, title = {Food size selectivity of Artemia franciscana at three developmental stages}, author = {P Makridis and O Vadstein}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0032698922&partnerID=40&md5=99fcdd04e89d1cdd683521fdf8f7e82f}, doi = {10.1093/plankt/21.11.2191}, issn = {14643774}, year = {1999}, date = {1999-01-01}, journal = {Journal of Plankton Research}, volume = {21}, number = {11}, pages = {2191--2201}, abstract = {Food size selectivity was examined in Artemia franciscana metanauplii at three different developmental stages. Clearance rates were determined in short-term experiments either by measuring the decrease in concentration of live particles and plastic beads, or by measuring the radioactivity accumulated in animals that grazed 14C-labelled live particles. The maximum clearance rate of A.franciscana metanauplii increased during development and was measured at 50-63 μl ind.-1 h-1, 254 μl ind.-1 h-1 and 1.48-2.10 ml ind.-1 h-1 in 2-, 4- and 7-day-old metanauplii, respectively. A preference for particles with a diameter of 4-8 μm was observed at all three developmental stages. The ability of A. franciscana metanauplii to graze bacterial particles was also demonstrated, although the efficiency in grazing such small particles was low compared to microalgae (28, 20 and 9% of the maximum clearance rate in 2-, 4- and 7-day-old metanauplii, respectively). Electron microscopy showed that the inter-setular distance in antennae and thoracopods was 0.20 ± 0.07, 0.16 ± 0.05 and 0.18 ± 0.04 μm in 2-, 4- and 7-day-old metanauplii, respectively, and accordingly independent of stage.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Food size selectivity was examined in Artemia franciscana metanauplii at three different developmental stages. Clearance rates were determined in short-term experiments either by measuring the decrease in concentration of live particles and plastic beads, or by measuring the radioactivity accumulated in animals that grazed 14C-labelled live particles. The maximum clearance rate of A.franciscana metanauplii increased during development and was measured at 50-63 μl ind.-1 h-1, 254 μl ind.-1 h-1 and 1.48-2.10 ml ind.-1 h-1 in 2-, 4- and 7-day-old metanauplii, respectively. A preference for particles with a diameter of 4-8 μm was observed at all three developmental stages. The ability of A. franciscana metanauplii to graze bacterial particles was also demonstrated, although the efficiency in grazing such small particles was low compared to microalgae (28, 20 and 9% of the maximum clearance rate in 2-, 4- and 7-day-old metanauplii, respectively). Electron microscopy showed that the inter-setular distance in antennae and thoracopods was 0.20 ± 0.07, 0.16 ± 0.05 and 0.18 ± 0.04 μm in 2-, 4- and 7-day-old metanauplii, respectively, and accordingly independent of stage. |
Rigos, G; Alexis, M; Nengas, I Leaching, palatability and digestibility of oxytetracycline and oxolinic acid included in diets fed to seabass Dicentrarchus labrax L. Journal Article Aquaculture Research, 30 , pp. 841 – 847, 1999. @article{rigos_leaching_1999, title = {Leaching, palatability and digestibility of oxytetracycline and oxolinic acid included in diets fed to seabass Dicentrarchus labrax L.}, author = {G Rigos and M Alexis and I Nengas}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0033385354&partnerID=40&md5=b697c8b14ba6b9a0fe7b97acc49786f2}, year = {1999}, date = {1999-01-01}, journal = {Aquaculture Research}, volume = {30}, pages = {841 -- 847}, abstract = {The leaching of oxolinic acid (OA) and oxytetracycline (OTC) from medicated feeds, as well as the palatability and digestibility of these drugs were investigated in seabass Dicentrarchus labrax L. (350g) at 16°C and 24°C water temperature. Oil- coated OA and OTC on feeds were greatly affected by leaching at 16 °C (losses of 55.5% and 42.5% for OA and OTC respectively) and 24 °C (32% for OA and 47% for OTC). However, a significant reduction in drug loss resulting from leaching was evident when the antibiotics were mixed with the diet at 16°C (5% for OA and 6.5% for OTC) and 24°C (10% and 20% for OA and OTC respectively). Increased water temperature induced a significant effect on the leaching of both drugs when mixed with the feed, but did not affect the loss of oil-coated drugs. There was a significant reduction in feed consumption of fish fed a diet with oil-coated OTC (90% and 92% at 16°C and 24°C respectively), whereas intake of feed containing oil-coated OA was only slightly affected (10% at 16 °C, 12% at 24 °C). The depressed palatability of feeds containing oil-coated OTC was overcome by mixing the drug with the diet. Both antibiotics were well digested by seabass (94% and 85% for OA and OTC respectively) at 24°C; however, more than one-third of each drug was recovered in the faeces at 16 °C (64% and 59% for OA and OTC respectively). The significance of these findings for the interaction between fish therapy and marine environment is stressed.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The leaching of oxolinic acid (OA) and oxytetracycline (OTC) from medicated feeds, as well as the palatability and digestibility of these drugs were investigated in seabass Dicentrarchus labrax L. (350g) at 16°C and 24°C water temperature. Oil- coated OA and OTC on feeds were greatly affected by leaching at 16 °C (losses of 55.5% and 42.5% for OA and OTC respectively) and 24 °C (32% for OA and 47% for OTC). However, a significant reduction in drug loss resulting from leaching was evident when the antibiotics were mixed with the diet at 16°C (5% for OA and 6.5% for OTC) and 24°C (10% and 20% for OA and OTC respectively). Increased water temperature induced a significant effect on the leaching of both drugs when mixed with the feed, but did not affect the loss of oil-coated drugs. There was a significant reduction in feed consumption of fish fed a diet with oil-coated OTC (90% and 92% at 16°C and 24°C respectively), whereas intake of feed containing oil-coated OA was only slightly affected (10% at 16 °C, 12% at 24 °C). The depressed palatability of feeds containing oil-coated OTC was overcome by mixing the drug with the diet. Both antibiotics were well digested by seabass (94% and 85% for OA and OTC respectively) at 24°C; however, more than one-third of each drug was recovered in the faeces at 16 °C (64% and 59% for OA and OTC respectively). The significance of these findings for the interaction between fish therapy and marine environment is stressed. |
Kotzabasis, K; Hatziathanasiou, A; Bengoa-Ruigomez, M V; Kentouri, M; Divanach, P Progress in Industrial Microbiology, 35 , pp. 357–362, Elsevier, 1999, ISBN: 978-0-444-50387-9. @incollection{kotzabasis_methanol_1999, title = {Methanol as alternative carbon source for quicker efficient production of the microalgae Chlorella minutissima: Role of the concentration and frequence of administration}, author = {K Kotzabasis and A Hatziathanasiou and M V Bengoa-Ruigomez and M Kentouri and P Divanach}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0079635299801283}, doi = {10.1016/S0079-6352(99)80128-3}, isbn = {978-0-444-50387-9}, year = {1999}, date = {1999-01-01}, urldate = {2020-08-18}, booktitle = {Progress in Industrial Microbiology}, volume = {35}, pages = {357--362}, publisher = {Elsevier}, abstract = {Autotrophic cultures of the marine microalgae Chlorella minutissima were performed at 13 000 lux continuous illumination in 11 chambers fertilised with 0.25 g l - 1 F2 medium and different doses of methanol. This was administered in two ways during two parallel experimental series of 10 days: 0.05, 0.1, 0.5, 1.0 and 5.0% methanol (v/v) in one unique dose at the beginning of the culture and 110 of these (i.e. 0.005, 0.01, 0.05, 0.1 and 0.5% methanol (v/v)) in daily doses for the 10-day culture period. Low concentrations of methanol induced a faster increase of cell density and dry weight than control, while high concentrations induced symptoms of toxicity. The higher cell densities and quicker growth were observed in the experiments with daily administration of 0.005 and 0.1% (v/v) methanol, while those with one dose presented an initial boosted growth but a final cell density lower than control. The role of methanol as alternative carbon source for microalgae, as well as its possible impact on the quality of biomass production and on the environment, are discussed. © 1999 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {incollection} } Autotrophic cultures of the marine microalgae Chlorella minutissima were performed at 13 000 lux continuous illumination in 11 chambers fertilised with 0.25 g l - 1 F2 medium and different doses of methanol. This was administered in two ways during two parallel experimental series of 10 days: 0.05, 0.1, 0.5, 1.0 and 5.0% methanol (v/v) in one unique dose at the beginning of the culture and 110 of these (i.e. 0.005, 0.01, 0.05, 0.1 and 0.5% methanol (v/v)) in daily doses for the 10-day culture period. Low concentrations of methanol induced a faster increase of cell density and dry weight than control, while high concentrations induced symptoms of toxicity. The higher cell densities and quicker growth were observed in the experiments with daily administration of 0.005 and 0.1% (v/v) methanol, while those with one dose presented an initial boosted growth but a final cell density lower than control. The role of methanol as alternative carbon source for microalgae, as well as its possible impact on the quality of biomass production and on the environment, are discussed. © 1999 Elsevier B.V. All rights reserved. |
Petihakis, G; Triantafyllou, G; Koutsoubas, D; Allen, I; Dounas, C Modelling the annual cycles of nutrients and phytoplankton in a Mediterranean lagoon (Gialova, Greece) Journal Article Marine Environmental Research, 48 (1), pp. 37–58, 1999, ISSN: 01411136. @article{petihakis_modelling_1999, title = {Modelling the annual cycles of nutrients and phytoplankton in a Mediterranean lagoon (Gialova, Greece)}, author = {G Petihakis and G Triantafyllou and D Koutsoubas and I Allen and C Dounas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032962205&doi=10.1016%2fS0141-1136%2899%2900031-8&partnerID=40&md5=8fed2e52e84b89476fe2f603648f92d2}, doi = {10.1016/S0141-1136(99)00031-8}, issn = {01411136}, year = {1999}, date = {1999-01-01}, journal = {Marine Environmental Research}, volume = {48}, number = {1}, pages = {37--58}, abstract = {Nutrient dynamics for phosphate, nitrate, ammonium and silicate have been simulated with the European Regional Seas Ecosystem Model in a Mediterranean lagoon. This generic model designed for the open sea can be usefully applied also to coastal lagoon ecosystems with minimum modifications. The annual cycles of the nutrients phosphate and silicate compare guile well with the observed ranges of variability. This does not hold for ammonium and nitrate where the increased concentrations could be attributed to external inputs from the land. Nutrient budgets calculated from the model results indicate some competition between phytoplankton and bacteria for nutrients, a common characteristic of lagoons. To further develop the model, an adaptation of the phytoplankton submodel to represent benthic primary production, as well as the modification of the benthic nutrient model to cope with anoxic events are suggested.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Nutrient dynamics for phosphate, nitrate, ammonium and silicate have been simulated with the European Regional Seas Ecosystem Model in a Mediterranean lagoon. This generic model designed for the open sea can be usefully applied also to coastal lagoon ecosystems with minimum modifications. The annual cycles of the nutrients phosphate and silicate compare guile well with the observed ranges of variability. This does not hold for ammonium and nitrate where the increased concentrations could be attributed to external inputs from the land. Nutrient budgets calculated from the model results indicate some competition between phytoplankton and bacteria for nutrients, a common characteristic of lagoons. To further develop the model, an adaptation of the phytoplankton submodel to represent benthic primary production, as well as the modification of the benthic nutrient model to cope with anoxic events are suggested. |
Rigos, G; Christophilogiannis, P; Yiagnisi, M; Andriopoulou, A; Koutsodimou, M; Nengas, I; Alexis, M Myxosporean infections in Greek mariculture Journal Article Aquaculture International, 7 , pp. 361 – 364, 1999. @article{rigos_myxosporean_1999, title = {Myxosporean infections in Greek mariculture}, author = {G Rigos and P Christophilogiannis and M Yiagnisi and A Andriopoulou and M Koutsodimou and I Nengas and M Alexis}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0033512619&partnerID=40&md5=b36f94c8f28917da682199a49d768f7b}, year = {1999}, date = {1999-01-01}, journal = {Aquaculture International}, volume = {7}, pages = {361 -- 364}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Pavlidis, M; Berry, M; Kokkari, C; Kentouri, M Prothrombin time, activated partial thromboplastin time and fibrinogen values in Mediterranean marine teleosts Journal Article Fish Physiology and Biochemistry, 21 (4), pp. 335–343, 1999, ISSN: 09201742. @article{pavlidis_prothrombin_1999, title = {Prothrombin time, activated partial thromboplastin time and fibrinogen values in Mediterranean marine teleosts}, author = {M Pavlidis and M Berry and C Kokkari and M Kentouri}, url = {http://link.springer.com/10.1023/A:1007882020754}, doi = {10.1023/A:1007882020754}, issn = {09201742}, year = {1999}, date = {1999-01-01}, urldate = {2020-08-18}, journal = {Fish Physiology and Biochemistry}, volume = {21}, number = {4}, pages = {335--343}, abstract = {Coagulation profiles for marine fish were determined with the standard global screening assays, the prothrombin time (PT) and activated partial thromboplastin time (APTT) using homologous tissue thromboplastin and phospholipid extracts to investigate species-specificity of brain thromboplastins, and to establish a base-line coagulation profile for marine fish of economical importance for Mediterranean aquaculture (sea bream, Sparus aurata, sea bass, Dicentrarchus labrax, red porgy, Pagrus pagrus, common dentex, Dentex dentex). Sea bass and sea bream displayed the shortest PT (9.0-15.0 s) and APTT (22.1-35.8 s) in comparison to the other species (PT: 24.8-37.2 s, APTT: 39.6-66.4 s). Red porgy displayed the lowest fibrinogen value (1.2-2.3 g 1 -1), while no differences were found among the other species (2.2-2.8 g 1 -1). It is concluded that the interaction of the tissue factor and plasma clotting factor is relatively species specific and that the use of homologous brain extracts is preferable for haemostasis study in marine fish.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Coagulation profiles for marine fish were determined with the standard global screening assays, the prothrombin time (PT) and activated partial thromboplastin time (APTT) using homologous tissue thromboplastin and phospholipid extracts to investigate species-specificity of brain thromboplastins, and to establish a base-line coagulation profile for marine fish of economical importance for Mediterranean aquaculture (sea bream, Sparus aurata, sea bass, Dicentrarchus labrax, red porgy, Pagrus pagrus, common dentex, Dentex dentex). Sea bass and sea bream displayed the shortest PT (9.0-15.0 s) and APTT (22.1-35.8 s) in comparison to the other species (PT: 24.8-37.2 s, APTT: 39.6-66.4 s). Red porgy displayed the lowest fibrinogen value (1.2-2.3 g 1 -1), while no differences were found among the other species (2.2-2.8 g 1 -1). It is concluded that the interaction of the tissue factor and plasma clotting factor is relatively species specific and that the use of homologous brain extracts is preferable for haemostasis study in marine fish. |
Tselepides, A; Akoumianaki, I; Papadopoulou, K -N; Podaras, D; Plaiti, W; Tholosan, O Seasonal variability and distribution of organic matter in surface and subsurface sediments of the Aegean Sea. Inproceedings Perpignan, France, 1999, (Publication Title: The 4th MTP workshop- MATER). @inproceedings{tselepides_seasonal_1999, title = {Seasonal variability and distribution of organic matter in surface and subsurface sediments of the Aegean Sea.}, author = {A Tselepides and I Akoumianaki and K -N Papadopoulou and D Podaras and W Plaiti and O Tholosan}, year = {1999}, date = {1999-01-01}, address = {Perpignan, France}, note = {Publication Title: The 4th MTP workshop- MATER}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Navajas, M; Lagnel, J; Fauvel, G; Moraes, De G Sequence variation of ribosomal Internal Transcribed Spacers (ITS) in commercially important Phytoseiidae mites Journal Article Experimental and Applied Acarology, 23 , pp. 851 – 859, 1999. @article{navajas_sequence_1999, title = {Sequence variation of ribosomal Internal Transcribed Spacers (ITS) in commercially important Phytoseiidae mites}, author = {M Navajas and J Lagnel and G Fauvel and G De Moraes}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0033226612&partnerID=40&md5=80cdc7f31aeda6c5bbf3e383924720ff}, year = {1999}, date = {1999-01-01}, journal = {Experimental and Applied Acarology}, volume = {23}, pages = {851 -- 859}, abstract = {Preliminary work is needed to assess the usefulness of different markers at different taxonomic scales when a new group is analyzed, such as the commercially important Phytoseiidae mites. We investigate here the level of sequence variation of the nuclear ribosomal spacers ITS 1 and 2 and the 5.8S gene in six species of Phytoseiidae: Neoseiulus californicus, N. fallacis, Euseius concordis, Metaseiulus occidentalis. Typhlodromus pyri and Phytoseiulus persimilis. As expected, the 5.8S gene (148 base pairs) is markedly conserved and displays little variation in between genera comparisons. ITS1 and ITS2 show contrasting patterns: while the ITS2 is short (80-89 bp) and shows little variation, the ITS1 is longer (303-404 bp) and is very variable in sequence. This fact compromises reliable nucleotide homologies when comparing the genera. The comparison of ITS1 sequence similarity at the species level might be useful for species identification, however, the value of ITS in taxonomic studies does not extend to the level of the family. The intraspecific variations of ITS were investigated in three species: N. californicus, N. fallacis and E. concordis. The first species has identical ITS1 sequences and the last two display low polymorphism (2 nucleotide substitutions). The ITS2 and 5.8S sequences were identical in all three subspecies comparisons.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Preliminary work is needed to assess the usefulness of different markers at different taxonomic scales when a new group is analyzed, such as the commercially important Phytoseiidae mites. We investigate here the level of sequence variation of the nuclear ribosomal spacers ITS 1 and 2 and the 5.8S gene in six species of Phytoseiidae: Neoseiulus californicus, N. fallacis, Euseius concordis, Metaseiulus occidentalis. Typhlodromus pyri and Phytoseiulus persimilis. As expected, the 5.8S gene (148 base pairs) is markedly conserved and displays little variation in between genera comparisons. ITS1 and ITS2 show contrasting patterns: while the ITS2 is short (80-89 bp) and shows little variation, the ITS1 is longer (303-404 bp) and is very variable in sequence. This fact compromises reliable nucleotide homologies when comparing the genera. The comparison of ITS1 sequence similarity at the species level might be useful for species identification, however, the value of ITS in taxonomic studies does not extend to the level of the family. The intraspecific variations of ITS were investigated in three species: N. californicus, N. fallacis and E. concordis. The first species has identical ITS1 sequences and the last two display low polymorphism (2 nucleotide substitutions). The ITS2 and 5.8S sequences were identical in all three subspecies comparisons. |
J., Iliopoulou-Georgudaki; P., Kaspiris; V., Kantzaris; Katharios, P Study of the seasonal fluctuation of the ecological quality of the Rivers: Kalamas, Acherontas, Alfios, Pinios with the use of benthic macro-invertebrates as bioindicators. Inproceedings of Sciences, Hellenic Society Biological (Ed.): 21st Conference of the Hellenic Society of Biological Sciences, pp. 91–92, Syros, Greece, 1999. @inproceedings{iliopoulou-georgudaki_study_1999, title = {Study of the seasonal fluctuation of the ecological quality of the Rivers: Kalamas, Acherontas, Alfios, Pinios with the use of benthic macro-invertebrates as bioindicators.}, author = {Iliopoulou-Georgudaki J. and Kaspiris P. and Kantzaris V. and P Katharios}, editor = {Hellenic Society Biological of Sciences}, year = {1999}, date = {1999-01-01}, booktitle = {21st Conference of the Hellenic Society of Biological Sciences}, pages = {91--92}, address = {Syros, Greece}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Tsigenopoulos, C S; Karakousis, Y; Berrebi, P The North Mediterranean Barbus lineage : phylogenetic hypotheses and taxonomic implications based on allozyme data Journal Article Journal of Fish Biology, 54 (2), pp. 267–286, 1999. @article{tsigenopoulos_north_1999, title = {The North Mediterranean Barbus lineage : phylogenetic hypotheses and taxonomic implications based on allozyme data}, author = {C S Tsigenopoulos and Y Karakousis and P Berrebi}, year = {1999}, date = {1999-01-01}, journal = {Journal of Fish Biology}, volume = {54}, number = {2}, pages = {267--286}, abstract = {Systematics of the European species of the genus textitBarbus is controversial for decades because of the relative similarity in general morphology between species. The use of more objective and precise characters, such as osteology, has given improved results. However, in the absence of a thorough morphological analysis of the group, genetic markers providing a promising method for obtaining more accurate phylogenies.On the basis of distinct ecological and morphological characters, the European textitBarbus taxa have been clustered in two groups : a fluvio-lacustrinetextittextless/itextgreaterand a rheophilic or strictly riverine one. These two groups (or ecophenotypes) were recognised in different parts of Europe, and formed, either a species assemblage (textlessitextgreaterBarbus barbus group) or a polytypic species (textitBarbus meridionalis). The implicit idea is that species of to the same group belong, to the same phylogenetic lineage (clade) and are the result of the same transcontinental colonisation event.The analysis, using allozyme markers, of 10 taxa of the genus textitBarbus from France, Italy, Greece, the Czech Republic and Slovakia, showed that the taxa thought to belong to the fluvio-lacustrine and the rheophilic groups are not monophyletic. We mention some alternative hypotheses, including the possibility that the allozyme markers may be inadequate. The results suggest that probably in each sub-region, the founding taxon has diverged independently to form species of two different ecophenotypes ; one occupying the upstream rivers and another found in the lowland rivers. Accordingly, textitBarbus species groups represent clusters of morphologically convergent taxa living in equivalent biotopes}, keywords = {}, pubstate = {published}, tppubtype = {article} } Systematics of the European species of the genus textitBarbus is controversial for decades because of the relative similarity in general morphology between species. The use of more objective and precise characters, such as osteology, has given improved results. However, in the absence of a thorough morphological analysis of the group, genetic markers providing a promising method for obtaining more accurate phylogenies.On the basis of distinct ecological and morphological characters, the European textitBarbus taxa have been clustered in two groups : a fluvio-lacustrinetextittextless/itextgreaterand a rheophilic or strictly riverine one. These two groups (or ecophenotypes) were recognised in different parts of Europe, and formed, either a species assemblage (textlessitextgreaterBarbus barbus group) or a polytypic species (textitBarbus meridionalis). The implicit idea is that species of to the same group belong, to the same phylogenetic lineage (clade) and are the result of the same transcontinental colonisation event.The analysis, using allozyme markers, of 10 taxa of the genus textitBarbus from France, Italy, Greece, the Czech Republic and Slovakia, showed that the taxa thought to belong to the fluvio-lacustrine and the rheophilic groups are not monophyletic. We mention some alternative hypotheses, including the possibility that the allozyme markers may be inadequate. The results suggest that probably in each sub-region, the founding taxon has diverged independently to form species of two different ecophenotypes ; one occupying the upstream rivers and another found in the lowland rivers. Accordingly, textitBarbus species groups represent clusters of morphologically convergent taxa living in equivalent biotopes |
Carrillo, J; Martinez, J; Divanach, P; Kentouri, M Unilateral eye abnormalities in reared Mediterranean gilthead sea bream Journal Article Veterinary Record, 145 , pp. 494 – 497, 1999. @article{carrillo_unilateral_1999, title = {Unilateral eye abnormalities in reared Mediterranean gilthead sea bream}, author = {J Carrillo and J Martinez and P Divanach and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0033598482&partnerID=40&md5=3137530bf8b7e7dcb44d7cc2eae77911}, doi = {http://dx.doi.org/10.1136/vr.145.17.494}, year = {1999}, date = {1999-01-01}, journal = {Veterinary Record}, volume = {145}, pages = {494 -- 497}, abstract = {Eye abnormalities in reared gilthead sea bream (Sparus aurato) were investigated clinically and by histological techniques. A significant number of fish had a cataract in one eye and ocular inflammation including gross exophthalmos. In a small number of fish gas bubbles were observed in the anterior chamber of the eye. Histological examination provided no evidence of an infectious process and a possible behavioural aetiology is discussed.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Eye abnormalities in reared gilthead sea bream (Sparus aurato) were investigated clinically and by histological techniques. A significant number of fish had a cataract in one eye and ocular inflammation including gross exophthalmos. In a small number of fish gas bubbles were observed in the anterior chamber of the eye. Histological examination provided no evidence of an infectious process and a possible behavioural aetiology is discussed. |
1998 |
Rueda, F M; Martinez, F J; Zamora, S; Kentouri, M; Divanach, P Effect of fasting and refeeding on growth and body composition of red porgy, Pagrus pagrus L.* Journal Article Aquaculture Research, 29 (6), pp. 447–452, 1998, ISSN: 1355-557X, 1365-2109. @article{rueda_effect_1998, title = {Effect of fasting and refeeding on growth and body composition of red porgy, Pagrus pagrus L.*}, author = {F M Rueda and F J Martinez and S Zamora and M Kentouri and P Divanach}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1046/j.1365-2109.1998.00228.x}, doi = {10.1046/j.1365-2109.1998.00228.x}, issn = {1355-557X, 1365-2109}, year = {1998}, date = {1998-06-01}, urldate = {2020-08-18}, journal = {Aquaculture Research}, volume = {29}, number = {6}, pages = {447--452}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Kristoffersen, Jon Bent; Salvanes, Anne Gro Vea Effects of formaldehyde and ethanol preservation on body and otoliths of Maurolicus muelleri and Benthosema glaciale Journal Article Sarsia, 83 (2), pp. 95–102, 1998, ISSN: 0036-4827. @article{kristoffersen_effects_1998, title = {Effects of formaldehyde and ethanol preservation on body and otoliths of Maurolicus muelleri and Benthosema glaciale}, author = {Jon Bent Kristoffersen and Anne Gro Vea Salvanes}, url = {http://www.tandfonline.com/doi/full/10.1080/00364827.1998.10413675}, doi = {10.1080/00364827.1998.10413675}, issn = {0036-4827}, year = {1998}, date = {1998-06-01}, urldate = {2020-08-18}, journal = {Sarsia}, volume = {83}, number = {2}, pages = {95--102}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Navajas, M; Lagnel, J; Gutierrez, J; Boursot, P Heredity, 80 (6), pp. 742–752, 1998, ISSN: 0018-067X, 1365-2540. @article{navajas_species-wide_1998, title = {Species-wide homogeneity of nuclear ribosomal ITS2 sequences in the spider mite Tetranychus urticae contrasts with extensive mitochondrial COI polymorphism}, author = {M Navajas and J Lagnel and J Gutierrez and P Boursot}, url = {http://www.nature.com/doifinder/10.1046/j.1365-2540.1998.00349.x}, doi = {10.1046/j.1365-2540.1998.00349.x}, issn = {0018-067X, 1365-2540}, year = {1998}, date = {1998-06-01}, urldate = {2020-08-18}, journal = {Heredity}, volume = {80}, number = {6}, pages = {742--752}, abstract = {We compared patterns of intraspecific polymorphism of two markers with contrasted modes of evolution, nuclear ribosomal DNA (rDNA) and mitochondrial DNA (mtDNA), in the phytophagous mite Tetranychus urticae Koch. The second internal transcribed spacer (ITS2) of rDNA and a fragment in the mtDNA gene coding for Cytochrome Oxidase I (COI), were PCR-amplified and sequenced in samples of various geographical origins distributed worldwide. The 15 COI haplotypes found fell into two major phylogenetic lineages differing by an average of 5% nucleotide divergence. Samples from the Mediterranean basin were represented in both lineages, and showed no phylogeographical structure. The other samples, from temperate regions of the northern hemisphere, were clustered in one of the lineages and displayed little variation, indicating a recent colonization of this region. In contrast, no variation at all was found at the ITS2 in this species. We sequenced both COI and ITS2 in four other species of the genus Tetranychus and found that, despite the absence of intraspecific polymorphism, ITS appears to evolve 2.5 times faster than COI. We argue that rDNA homogeneity over the species range of T. urticae results from the high colonization potential of this species, preventing long-term differentiation. Preliminary data on two other mite species (Amphitetranychus viennensis Zacher and Mononychellus progresivus Doreste) with stricter ecological requirements and more restricted colonization potential revealed substantial and concordant geographical differentiation for both ITS2 and COI.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We compared patterns of intraspecific polymorphism of two markers with contrasted modes of evolution, nuclear ribosomal DNA (rDNA) and mitochondrial DNA (mtDNA), in the phytophagous mite Tetranychus urticae Koch. The second internal transcribed spacer (ITS2) of rDNA and a fragment in the mtDNA gene coding for Cytochrome Oxidase I (COI), were PCR-amplified and sequenced in samples of various geographical origins distributed worldwide. The 15 COI haplotypes found fell into two major phylogenetic lineages differing by an average of 5% nucleotide divergence. Samples from the Mediterranean basin were represented in both lineages, and showed no phylogeographical structure. The other samples, from temperate regions of the northern hemisphere, were clustered in one of the lineages and displayed little variation, indicating a recent colonization of this region. In contrast, no variation at all was found at the ITS2 in this species. We sequenced both COI and ITS2 in four other species of the genus Tetranychus and found that, despite the absence of intraspecific polymorphism, ITS appears to evolve 2.5 times faster than COI. We argue that rDNA homogeneity over the species range of T. urticae results from the high colonization potential of this species, preventing long-term differentiation. Preliminary data on two other mite species (Amphitetranychus viennensis Zacher and Mononychellus progresivus Doreste) with stricter ecological requirements and more restricted colonization potential revealed substantial and concordant geographical differentiation for both ITS2 and COI. |
Publications
2000 |
Effects of three preservation methods on the evolution of swimbladder radiographic appearance in sea bass and sea bream juveniles Journal Article Aquaculture, 182 , pp. 17 – 25, 2000. |
Evidence of temperature-dependent sex determination in the european sea bass (Dicentrarchus labrax L.) Journal Article Journal of Experimental Zoology, 287 , pp. 225 – 232, 2000. |
Feeding requirements of sea bream (Sparus aurata) larvae under intensive rearing conditions: Development of a fuzzy logic controller for feeding Journal Article Aquacultural Engineering, 21 , pp. 285 – 299, 2000. |
Fish farming design Incollection Castritsi-Catharios, J (Ed.): Current knowledge in Aquaculture, Students’ Handbook, pp. Β 1–11, University publications, Athens, 2000, (Backup Publisher: University publications). |
Genetic differentiation in Tetranychus urticae (Acari: Tetranychidae): Polymorphism, host races or sibling species? Journal Article Experimental and Applied Acarology, 24 , pp. 365 – 376, 2000. |
Impact of organic enrichment on the vertical distribution and the size of macrofauna in a coastal fish farming size. Inproceedings pp. 341–345, Chios, Greece, 2000, (Publication Title: The 6th Hellenic Symposium of Oceanography and Fisheries,). |
Experimental and Applied Acarology, 24 (10/11), pp. 795–803, 2000, ISSN: 01688162. |
Macrobenthic community structure and disturbance assessment in Gialova Lagoon, Ionian Sea Journal Article ICES Journal of Marine Science, 57 (5), pp. 1472–1480, 2000, ISSN: 10543139, (Publisher: Academic Press). |
Mitochondrial and nuclear DNA phylogeography of Crassostrea angulata, the Portuguese oyster endangered in Europe Journal Article Conservation Genetics, 1 (3), pp. 251–262, 2000, ISSN: 15660621. |
Molecular Phylogenetics and Evolution, 14 , pp. 165 – 179, 2000. |
Polychaete fauna of the Aegean Sea: Inventory and new information Journal Article Bulletin of Marine Science, 66 (1), pp. 73–96, 2000, ISSN: 00074977, (Publisher: Rosenstiel School of Marine and Atmospheric Science). |
Seasonal and bathymetric trends of the amphipod fauna in Heraklion Bay (Aegean Sea) Journal Article Polskie Archiwum Hydrobiologii, 47 , pp. 517 – 525, 2000. |
General and Comparative Endocrinology, 118 , pp. 14 – 25, 2000. |
Genetical Research, 76 (3), pp. 273–284, 2000, ISSN: 00166723. |
Spatial variability in water column and sediment chemistry in Heraklion harbour (Crete, E. Mediterranean Journal Article Fresenius Envir Bull, 9 , pp. 164–171, 2000. |
Study of the bacteriostatic activity of an Artemia enrichment compound based on plant extracts from Angelica sp Journal Article Bulletin of the Hellenic Veterinary Medical Society, 51 , pp. 293–296, 2000. |
Temporal variations in benthic communities and their response to physicochemical forcing: A numerical approach Journal Article ICES Journal of Marine Science, 57 (5), pp. 1507–1516, 2000, ISSN: 10543139, (Publisher: Academic Press). |
The effect of glycerol dissolved in the rearing water on the transition to exotrophy in gilthead sea bream Sparus aurata larvae Journal Article Aquaculture, 189 , pp. 119 – 131, 2000. |
The meiofaunal community structure of a Mediterranean lagoon (Gialova lagoon, Ionian Sea) Journal Article Helgoland Marine Research, 54 (1), pp. 7–17, 2000, ISSN: 1438387X, (Publisher: Springer Verlag). |
Toxicity and digestibility of fumagillin DCH in gilthead sea bream, Sparus aurata L. Journal Article Journal of Fish Diseases, 23 , pp. 161 – 164, 2000. |
Toxicity of intraperitoneal administered ivermectin in sea bream, Sparus aurata. Inproceedings of Sciences, Hellenic Society Biological (Ed.): pp. 56–60, Chios, Greece, 2000. |
Use of GnRHa-delivery systems for the control of reproduction in fish Journal Article Reviews in Fish Biology and Fisheries, 10 (4), pp. 463–491, 2000, ISSN: 09603166. |
1999 |
Nonlocal wind-driven fjord-coast advection and its potential effect on plankton and fish recruitment Journal Article Fisheries Oceanography, 8 (4), pp. 255–263, 1999, ISSN: 1054-6006, 1365-2419. |
Ontogeny and allometric plasticity of Dentex dentex (Osteichthyes: Sparidae) in rearing conditions Journal Article Marine Biology, 135 (3), pp. 561–572, 1999, ISSN: 0025-3162, 1432-1793. |
Growth, life history and population genetics of Benthosema glacial in fjordic and oceanic environments Inproceedings Kristoffersen, JB (Ed.): Gilleleje, Denmark, 1999. |
High inclusion levels of poultry meals and related byproducts in diets for gilthead seabream Sparus aurata L. Journal Article Aquaculture, 179 (1-4), pp. 13–23, 1999, ISSN: 00448486. |
Aquaculture, 179 (1-4), pp. 447–456, 1999, ISSN: 00448486. |
Mechanisms of insecticide resistance in the aphid Nasonovia ribisnigri (Mosley) (Homoptera: Aphididae) from France Journal Article Insect Biochemistry and Molecular Biology, 29 (4), pp. 385–391, 1999, ISSN: 09651748. |
Protein depletion of the rotifer Brachionus plicatilis during starvation Journal Article Aquaculture, 174 (3-4), pp. 343–353, 1999, ISSN: 00448486. |
Entomologia Experimentalis Et Applicata, 90 (2), pp. 113–122, 1999, (ISBN: 0013-8703). |
Osteological development of the vertebral column and of the caudal complex in Dentex dentex Journal Article Journal of Fish Biology, 54 (2), pp. 424–436, 1999, ISSN: 0022-1112, 1095-8649. |
Annelid fauna of a Mediterranean lagoon (Gialova Lagoon, south-west Greece): Community structure in a severely fluctuating environment Journal Article Journal of the Marine Biological Association of the United Kingdom, 79 (5), pp. 849–856, 1999, ISSN: 00253154. |
Benthic recovery following cessation of fish farming:a series of successes and catastrophes Journal Article Marine Ecology Progress Series, 184 , pp. 205–218, 1999, ISSN: 0171-8630, 1616-1599. |
Characterization of six polymorphic microsatellite markers in gilthead sea bream, Sparus aurata (Linnaeus 1758) Journal Article Molecular Ecology, 8 , pp. 897–898, 1999. |
Cryopreservation of microalgae and toxicity of cryoprotectants for aquaculture Inproceedings Proceedings 21st Panhellenic Meeting of Hellenic Society of Biological Sciences, Syros, Greece, 1999, (Publication Title: Proceedings 21st Panhellenic Meeting of Hellenic Society of Biological Sciences). |
Diel rhythms of serum metabolites and thyroid hormones in red porgy held in different photoperiod regimes Journal Article Aquaculture International, 7 , pp. 29 – 44, 1999. |
Food size selectivity of Artemia franciscana at three developmental stages Journal Article Journal of Plankton Research, 21 (11), pp. 2191–2201, 1999, ISSN: 14643774. |
Leaching, palatability and digestibility of oxytetracycline and oxolinic acid included in diets fed to seabass Dicentrarchus labrax L. Journal Article Aquaculture Research, 30 , pp. 841 – 847, 1999. |
Progress in Industrial Microbiology, 35 , pp. 357–362, Elsevier, 1999, ISBN: 978-0-444-50387-9. |
Modelling the annual cycles of nutrients and phytoplankton in a Mediterranean lagoon (Gialova, Greece) Journal Article Marine Environmental Research, 48 (1), pp. 37–58, 1999, ISSN: 01411136. |
Myxosporean infections in Greek mariculture Journal Article Aquaculture International, 7 , pp. 361 – 364, 1999. |
Prothrombin time, activated partial thromboplastin time and fibrinogen values in Mediterranean marine teleosts Journal Article Fish Physiology and Biochemistry, 21 (4), pp. 335–343, 1999, ISSN: 09201742. |
Seasonal variability and distribution of organic matter in surface and subsurface sediments of the Aegean Sea. Inproceedings Perpignan, France, 1999, (Publication Title: The 4th MTP workshop- MATER). |
Sequence variation of ribosomal Internal Transcribed Spacers (ITS) in commercially important Phytoseiidae mites Journal Article Experimental and Applied Acarology, 23 , pp. 851 – 859, 1999. |
Study of the seasonal fluctuation of the ecological quality of the Rivers: Kalamas, Acherontas, Alfios, Pinios with the use of benthic macro-invertebrates as bioindicators. Inproceedings of Sciences, Hellenic Society Biological (Ed.): 21st Conference of the Hellenic Society of Biological Sciences, pp. 91–92, Syros, Greece, 1999. |
The North Mediterranean Barbus lineage : phylogenetic hypotheses and taxonomic implications based on allozyme data Journal Article Journal of Fish Biology, 54 (2), pp. 267–286, 1999. |
Unilateral eye abnormalities in reared Mediterranean gilthead sea bream Journal Article Veterinary Record, 145 , pp. 494 – 497, 1999. |
1998 |
Effect of fasting and refeeding on growth and body composition of red porgy, Pagrus pagrus L.* Journal Article Aquaculture Research, 29 (6), pp. 447–452, 1998, ISSN: 1355-557X, 1365-2109. |
Effects of formaldehyde and ethanol preservation on body and otoliths of Maurolicus muelleri and Benthosema glaciale Journal Article Sarsia, 83 (2), pp. 95–102, 1998, ISSN: 0036-4827. |
Heredity, 80 (6), pp. 742–752, 1998, ISSN: 0018-067X, 1365-2540. |