Publications

@article{bailly_introduction_2016,

title = {Introduction to the Greek Taxon Information System (GTIS) in LifeWatchGreece: The construction of the Preliminary Checklists of Species of Greece},

author = {N Bailly and V Gerovasileiou and C Arvanitidis and A Legakis},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85018650313&doi=10.3897%2fBDJ.4.e7959&partnerID=40&md5=1f6351c142359acb9fee6b2e29b73393},

doi = {10.3897/BDJ.4.e7959},

issn = {13142828},

year  = {2016},

date = {2016-01-01},

journal = {Biodiversity Data Journal},

volume = {4},

number = {1},

abstract = {The Greek Taxon Information System is an initiative of the LifeWatchGreece Research Infrastructure (ESFRI) that is resuming efforts to compile a complete checklist of all species reported from the Greek territory. Such an effort is necessary as a requirement for all signatories of the Convention on Biological Diversity (Greece is a signatory since 1994). Over an estimation published in 2004 according to which 50,000 species are present in Greece, belonging to most kingdoms except bacteria and viruses, a list of 35,000 valid species (and subspecies) has been assembled from previous national and European initiatives and specialized databases on various groups. A new database will be progressively set up in the LifeWatchGreece Infrastructure within the near future. Before the dissemination of this dataset, it is important that the checklists will be validated by specialists for each taxonomic group. The first step already accomplished was to build and publish Preliminary Checklists for some taxonomic groups of marine fauna, which have been validated by specialists on the basis of their expertise and secondary literature. The publication of these Preliminary Checklists is expected to increase the visibility and usability of the database in the future not only to the scientific community but also to the broader domain of biodiversity management, especially in cases where no such checklists have been published yet. The guidelines used to test the first taxonomic groups are presented in this paper. © Bailly N et al.},

note = {Publisher: Pensoft Publishers},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{anastasiou_imposex_2016,

title = {Imposex and organotin compounds in ports of the Mediterranean and the Atlantic: Is the story over?},

author = {T I Anastasiou and E Chatzinikolaou and M Mandalakis and C Arvanitidis},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84978807299&doi=10.1016%2fj.scitotenv.2016.06.209&partnerID=40&md5=f0a46f0f9141ca86261409181b4d0d9b},

doi = {10.1016/j.scitotenv.2016.06.209},

issn = {00489697},

year  = {2016},

date = {2016-01-01},

journal = {Science of the Total Environment},

volume = {569-570},

pages = {1315--1329},

abstract = {Organotin compounds are toxic substances released into the marine environment from antifouling paints. Sixty-two years following their first application and 9 years after the complete ban on their use as biocides in 2003 (EC No. 782/2003), their negative impact on mollusks was still evident, as illustrated by imposex (i.e. the masculinization of female gastropods). This phenomenon is widely used to investigate tributyltin (TBT) pollution, with Hexaplex trunculus and Nassarius nitidus being considered as sensitive bioindicators. H. trunculus specimens and sediment samples collected from the ports of Cagliari (Sardinia), El Kantaoui (Tunisia) and Olhão (Portugal), along with N. nitidus individuals from the port of Faro (Portugal), were used for the assessment of imposex intensity and organotin pollution in these areas. High imposex frequency and organotin concentrations (TBT, triphenyltin (TPhT) and their degradation products) were observed, especially in Sardinia, implying that these chemicals remain a significant pollution issue in the specific Mediterranean ports. Moreover, the highly significant relationship established between penis length of imposex-affected females H. trunculus and TBT/TPhT concentrations offers a rapid and nonsacrificial proxy model for assessing the impact of organotins in coastal environments. Finally, the complementary use of more than one TBT bioindicator is advised in order to obtain more accurate results in detailed monitoring studies of TBT pollution. © 2016 Elsevier B.V.},

note = {Publisher: Elsevier B.V.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{vasileiadou_environmental_2016,

title = {Environmental variability and heavy metal concentrations from five lagoons in the Ionian Sea (Amvrakikos Gulf, W Greece)},

author = {K Vasileiadou and C Pavloudi and I Kalantzi and E T Apostolaki and G Chatzigeorgiou and E Chatzinikolaou and E Pafilis and N Papageorgiou and L Fanini and S Konstas and N Fragopoulou and C Arvanitidis},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85018621471&doi=10.3897%2fBDJ.4.e8233&partnerID=40&md5=91104fbd24f94063ee946df8c2486376},

doi = {10.3897/BDJ.4.e8233},

issn = {13142828},

year  = {2016},

date = {2016-01-01},

journal = {Biodiversity Data Journal},

volume = {4},

number = {1},

abstract = {Background Coastal lagoons are ecosystems of major importance as they host a number of species tolerant to disturbances and they are highly productive. Therefore, these ecosystems should be protected to ensure stability and resilience. The lagoons of Amvrakikos Gulf form one of the most important lagoonal complexes in Greece. The optimal ecological status of these lagoons is crucial for the well-being of the biodiversity and the economic prosperity of the local communities. Thus, monitoring of the area is necessary to detect possible sources of disturbance and restore stability. New information The environmental variables and heavy metals concentrations, from five lagoons of Amvrakikos Gulf were measured from seasonal samplings and compared to the findings of previous studies in the area, in order to check for possible sources of disturbance. The analysis, showed that i) the values of the abiotic parameters vary with time (season), space (lagoon) and with space over time; ii) the variability of the environmental factors and enrichment in certain elements is naturally induced and no source of contamination is detected in the lagoons. © Vasileiadou K et al.},

note = {Publisher: Pensoft Publishers},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{varsos_optimized_2016,

title = {Optimized R functions for analysis of ecological community data using the R virtual laboratory (RvLab)},

author = {C Varsos and T Patkos and A Oulas and C Pavloudi and A Gougousis and U Z Ijaz and I Filiopoulou and N Pattakos and E V Berghe and A Fernández-Guerra and S Faulwetter and E Chatzinikolaou and E Pafilis and C Bekiari and M Doerr and C Arvanitidis},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85018640003&doi=10.3897%2fBDJ.4.e8357&partnerID=40&md5=6003e9caf6582fdde7f4bffec91e154f},

doi = {10.3897/BDJ.4.e8357},

issn = {13142828},

year  = {2016},

date = {2016-01-01},

journal = {Biodiversity Data Journal},

volume = {4},

number = {1},

abstract = {Background Parallel data manipulation using R has previously been addressed by members of the R community, however most of these studies produce ad hoc solutions that are not readily available to the average R user. Our targeted users, ranging from the expert ecologist/ microbiologists to computational biologists, often experience difficulties in finding optimal ways to exploit the full capacity of their computational resources. In addition, improving performance of commonly used R scripts becomes increasingly difficult especially with large datasets. Furthermore, the implementations described here can be of significant interest to expert bioinformaticians or R developers. Therefore, our goals can be summarized as: (i) description of a complete methodology for the analysis of large datasets by combining capabilities of diverse R packages, (ii) presentation of their application through a virtual R laboratory (RvLab) that makes execution of complex functions and visualization of results easy and readily available to the end-user. New information In this paper, the novelty stems from implementations of parallel methodologies which rely on the processing of data on different levels of abstraction and the availability of these processes through an integrated portal. Parallel implementation R packages, such as the pbdMPI (Programming with Big Data - Interface to MPI) package, are used to implement Single Program Multiple Data (SPMD) parallelization on primitive mathematical operations, allowing for interplay with functions of the vegan package. The dplyr and RPostgreSQL R packages are further integrated offering connections to dataframe like objects (databases) as secondary storage solutions whenever memory demands exceed available RAM resources. The RvLab is running on a PC cluster, using version 3.1.2 (2014-10-31) on a x86_64-pclinux- gnu (64-bit) platform, and offers an intuitive virtual environmet interface enabling users to perform analysis of ecological and microbial communities based on optimized vegan functions. A beta version of the RvLab is available after registration at: https:// portal.lifewatchgreece.eu/.},

note = {Publisher: Pensoft Publishers},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{minadakis_lifewatch_2016,

title = {LifeWatch Greece data-services: Discovering biodiversity data using semantic web technologies},

author = {N Minadakis and Y Marketakis and M Doerr and C Bekiari and P Papadakos and A Gougousis and N Bailly and C Arvanitidis},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85018630619&doi=10.3897%2fBDJ.4.e8443&partnerID=40&md5=e7d3559ed33e73168bed99db15f46b3d},

doi = {10.3897/BDJ.4.e8443},

issn = {13142828},

year  = {2016},

date = {2016-01-01},

journal = {Biodiversity Data Journal},

volume = {4},

number = {1},

abstract = {Background Biodiversity data is characterized by its cross-disciplinary character, the extremely broad range of data types and structures, and the variety of semantic concepts that it encompasses. Furthermore there is a plethora of different data sources providing resources for the same piece of information in a heterogeneous way. Even if we restrict our attention to Greek biodiversity domain, it is easy to see that biodiversity data remains unconnected and widely distributed among different sources. New information To cope with these issues, in the context of the LifeWatch Greece project, i) we supported cataloguing and publishing of all the relevant metadata information of the Greek biodiversity domain, ii) we integrated data from heterogeneous sources by supporting the definitions of appropriate models, iii) we provided means for efficiently discovering biodiversity data of interest and iv) we enabled the answering of complex queries that could not be answered from the individual sources. This work has been exploited, evaluated and scientificaly confirmed by the biodiversity community through the services provided by the LifeWatch Greece portal. © Minadakis N et al.},

note = {Publisher: Pensoft Publishers},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{chatzigeorgiou_testing_2016,

title = {Testing the robustness of Citizen Science projects: Evaluating the results of pilot project COMBER},

author = {G Chatzigeorgiou and S Faulwetter and T Dailianis and V S Smith and P Koulouri and C Dounas and C Arvanitidis},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85018623784&doi=10.3897%2fBDJ.4.e10859&partnerID=40&md5=5e1065f8df7260a055f72bdac84bccf2},

doi = {10.3897/BDJ.4.e10859},

issn = {13142828},

year  = {2016},

date = {2016-01-01},

journal = {Biodiversity Data Journal},

volume = {4},

number = {1},

abstract = {Background Citizen Science (CS) as a term implies a great deal of approaches and scopes involving many different fields of science. The number of the relevant projects globally has been increased significantly in the recent years. Large scale ecological questions can be answered only through extended observation networks and CS projects can support this effort. Although the need of such projects is apparent, an important part of scientific community cast doubt on the reliability of CS data sets. New information The pilot CS project COMBER has been created in order to provide evidence to answer the aforementioned question in the coastal marine biodiversity monitoring. The results of the current analysis show that a carefully designed CS project with clear hypotheses, wide participation and data sets validation, can be a valuable tool for the large scale and long term changes in marine biodiversity pattern change and therefore for relevant management and conservation issues. © Chatzigeorgiou G et al.},

note = {Publisher: Pensoft Publishers},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pearson_conflicts_2016,

title = {Conflicts in some of the World harbours: what needs to happen next?},

author = {S Pearson and W Windupranata and S W Pranowo and A Putri and Y Ma and A Vila-Concejo and E Fernández and G Méndez and J Banks and A M Knights and L B Firth and B B Breen and R Jarvis and J D Aguirre and S Chen and A N H Smith and P Steinberg and E Chatzinikolaou and C Arvanitidis},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84983239161&doi=10.1186%2fs40152-016-0049-x&partnerID=40&md5=39cb6bb169026a893bc8dac361e65934},

doi = {10.1186/s40152-016-0049-x},

issn = {18727859},

year  = {2016},

date = {2016-01-01},

journal = {Maritime Studies},

volume = {15},

number = {1},

abstract = {Harbours are a focus of intensive and diverse activities and thus have a high potential to become centres of conflict between users. Reviewing the multiple uses associated with harbours provides important insights into maritime communities and the management of conflict. In this paper, seven international, multi-disciplinary groups provide their expert synthesis of individual harbours. After a detailed discussion experts from Sydney, Qingdao, Vigo, Auckland, Jakarta, Crete and Plymouth synthesised and shared their harbour’s characteristics, user conflicts and how such conflicts have been researched and managed. The paper addresses an omission of “conflict” in most of the research literature about harbours, and ports and scopes a research agenda that includes integration, risk appreciation and other approaches to these increasingly contentious maritime environments. This process provided an opportunity for global researchers to share the ways harbour conflicts are mitigated and the kinds of adaptations that are possible. © 2016, Pearson et al.},

note = {Publisher: Springer Verlag},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nagasawa_substantial_2016,

title = {Substantial downregulation of myogenic transcripts in skeletal muscle of Atlantic cod during the spawning period.},

author = {Kazue Nagasawa and Elena Sarropoulou and Vigdis Edvardsen and Jorge M O Fernandes},

url = {https://dx.plos.org/10.1371/journal.pone.0148374},

doi = {DOI:10.1371/journal.pone.014837},

issn = {1932-6203},

year  = {2016},

date = {2016-01-01},

journal = {PLoS ONE},

volume = {11},

number = {2},

pages = {e0148374},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pavloudi_salinity_2016,

title = {Salinity is the major factor influencing the sediment bacterial communities in a Mediterranean lagoonal complex (Amvrakikos Gulf, Ionian Sea)},

author = {C Pavloudi and A Oulas and K Vasileiadou and E Sarropoulou and G Kotoulas and C Arvanitidis},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84955611416&doi=10.1016%2fj.margen.2016.01.005&partnerID=40&md5=a226c6872c0a3044831db4a1b5f65ca3},

doi = {10.1016/j.margen.2016.01.005},

issn = {18747787},

year  = {2016},

date = {2016-01-01},

journal = {Marine Genomics},

volume = {28},

pages = {71--81},

abstract = {Lagoons are naturally enriched habitats, with unstable environmental conditions caused by their confinement, shallow depth and state of saprobity. The frequent fluctuations of the abiotic variables cause severe changes in the abundance and distribution of biota. This relationship has been studied extensively for the macrofaunal communities, but not sufficiently so for the bacterial ones. The aim of the present study was to explore the biodiversity patterns of bacterial assemblages and to examine whether these patterns are associated with biogeographic and environmental factors. For this purpose, sediment samples were collected from five lagoons located in the Amvrakikos Gulf (Ionian Sea, Western Greece). DNA was extracted from the sediment and was further processed through 16S rRNA pyrosequencing. The results of this exploratory study imply that salinity is the environmental factor best correlated with the bacterial community pattern, which has also been suggested in similar studies but for macrofaunal community patterns. In addition, the bacterial community of the brackish lagoons is differentiated from that of the brackish-marine lagoons. The findings of this study indicate that the studied lagoons have distinct bacterial communities. © 2016 Elsevier B.V.},

note = {Publisher: Elsevier B.V.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{mavraki_rescuing_2016,

title = {Rescuing biogeographic legacy data: The "Thor" Expedition, a historical oceanographic expedition to the Mediterranean Sea},

author = {D Mavraki and L Fanini and M Tsompanou and V Gerovasileiou and S Nikolopoulou and E Chatzinikolaou and W Plaitis and S Faulwetter},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85018653928&doi=10.3897%2fBDJ.4.e11054&partnerID=40&md5=d548ea13a533ece32aee66df08954a37},

doi = {10.3897/BDJ.4.e11054},

issn = {13142828},

year  = {2016},

date = {2016-01-01},

journal = {Biodiversity Data Journal},

volume = {4},

number = {1},

abstract = {Background This article describes the digitization of a series of historical datasets based ?n the reports of the 1908-1910 Danish Oceanographical Expeditions to the Mediterranean and adjacent seas. All station and sampling metadata as well as biodiversity data regarding calcareous rhodophytes, pelagic polychaetes, and fish (families Engraulidae and Clupeidae) obtained during these expeditions were digitized within the activities of the LifeWatchGreece Research ?nfrastructure project and presented in the present paper. The aim was to safeguard public data availability by using an open access infrastructure, and to prevent potential loss of valuable historical data on the Mediterranean marine biodiversity. New information The datasets digitized here cover 2,043 samples taken at 567 stations during a time period from 1904 to 1930 in the Mediterranean and adjacent seas. The samples resulted in 1,588 occurrence records of pelagic polychaetes, fish (Clupeiformes) and calcareous algae (Rhodophyta). In addition, basic environmental data (e.g. sea surface temperature, salinity) as well as meterological conditions are included for most sampling events. In addition to the description of the digitized datasets, a detailed description of the problems encountered during the digitization of this historical dataset and a discussion on the value of such data are provided. © Mavraki D et al.},

note = {Publisher: Pensoft Publishers},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{keklikoglou_micro-ctvlab_2016,

title = {Micro-CTvlab: A web based virtual gallery of biological specimens using X-ray microtomography (micro-CT)},

author = {K Keklikoglou and S Faulwetter and E Chatzinikolaou and N Michalakis and I Filiopoulou and N Minadakis and E Panteri and G Perantinos and A Gougousis and C Arvanitidis},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85018643360&doi=10.3897%2fBDJ.4.e8740&partnerID=40&md5=450c2ceab515e072b612c9c046e0c7bc},

doi = {10.3897/BDJ.4.e8740},

issn = {13142828},

year  = {2016},

date = {2016-01-01},

journal = {Biodiversity Data Journal},

volume = {4},

number = {1},

abstract = {Background During recent years, X-ray microtomography (micro-CT) has seen an increasing use in biological research areas, such as functional morphology, taxonomy, evolutionary biology and developmental research. Micro-CT is a technology which uses X-rays to create submicron resolution images of external and internal features of specimens. These images can then be rendered in a three-dimensional space and used for qualitative and quantitative 3D analyses. However, the online exploration and dissemination of micro-CT datasets are rarely made available to the public due to their large size and a lack of dedicated online platforms for the interactive manipulation of 3D data. Here, the development of a virtual micro-CT laboratory (Micro-CTvlab ) is described, which can be used by everyone who is interested in digitisation methods and biological collections and aims at making the micro- CT data exploration of natural history specimens freely available over the internet. New information The Micro-CTvlab offers to the user virtual image galleries of various taxa which can be displayed and downloaded through a web application. With a few clicks, accurate, detailed and three-dimensional models of species can be studied and virtually dissected without destroying the actual specimen. The data and functions of the Micro-CTvlab can be accessed either on a normal computer or through a dedicated version for mobile devices. © Keklikoglou K et al.},

note = {Publisher: Pensoft Publishers},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{oulas_metagenomic_2016,

title = {Metagenomic investigation of the geologically unique Hellenic Volcanic Arc reveals a distinctive ecosystem with unexpected physiology.},

author = {Anastasis Oulas and Paraskevi N Polymenakou and Rekha Seshadri and James H Tripp and Manolis Mandalakis and David A Paez-Espino and Amrita Pati and Patrick Chain and Paraskevi Nomikou and Steven Carey and Stephanos Kilias and Christos Christakis and Georgios Kotoulas and Antonios Magoulas and Natalia N Ivanova and Nikos C Kyrpides},

url = {http://www.ncbi.nlm.nih.gov/pubmed/26487573},

doi = {10.1111/1462-2920.13095},

issn = {1462-2920},

year  = {2016},

date = {2016-01-01},

journal = {Environmental Microbiology},

volume = {18},

number = {4},

pages = {1122--1136},

abstract = {Hydrothermal vents represent a deep, hot, aphotic biosphere where chemosynthetic primary producers, fuelled by chemicals from Earth's subsurface, form the basis of life. In this study, we examined microbial mats from two distinct volcanic sites within the Hellenic Volcanic Arc (HVA). The HVA is geologically and ecologically unique, with reported emissions of CO2 -saturated fluids at temperatures up to 220°C and a notable absence of macrofauna. Metagenomic data reveals highly complex prokaryotic communities composed of chemolithoautotrophs, some methanotrophs, and to our surprise, heterotrophs capable of anaerobic degradation of aromatic hydrocarbons. Our data suggest that aromatic hydrocarbons may indeed be a significant source of carbon in these sites, and instigate additional research into the nature and origin of these compounds in the HVA. Novel physiology was assigned to several uncultured prokaryotic lineages; most notably, a SAR406 representative is attributed with a role in anaerobic hydrocarbon degradation. This dataset, the largest to date from submarine volcanic ecosystems, constitutes a significant resource of novel genes and pathways with potential biotechnological applications.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{guinand_candidate_2016,

title = {Candidate gene variation in gilthead sea bream reveals complex spatiotemporal selection patterns between marine and lagoon habitats},

author = {B Guinand and C Chauvel and M Lechene and J Tournois and C S Tsigenopoulos and A M Darnaude and D J McKenzie and P A Gagnaire},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84992490269&doi=10.3354%2fmeps11851&partnerID=40&md5=33291011e97c8e63e16a9b4feb4669d1},

doi = {10.3354/meps11851},

issn = {01718630},

year  = {2016},

date = {2016-01-01},

journal = {Marine Ecology Progress Series},

volume = {558},

pages = {115--127},

abstract = {In marine fishes, the extent to which spatial patterns induced by selection remain stable across generations remains largely unknown. In the gilthead sea bream Sparus aurata, polymorphisms in the growth hormone (GH) and prolactin (Prl) genes can display high levels of differentiation between marine and lagoon habitats. These genotype-environment associations have been attributed to differential selection following larval settlement, but it remains unclear whether selective mortality during later juvenile stages further shapes genetic differences among habitats. We addressed this question by analysing differentiation patterns at GH and Prl markers together with a set of 21 putatively neutral microsatellite loci. We compared genetic variation of spring juveniles that had just settled in 3 ecologically different lagoons against older juveniles sampled from the same sites in autumn, at the onset of winter outmigration. In spring, genetic differentiation among lagoons was greater than expected from neutrality for both candidate gene markers. Surprisingly, this signal disappeared completely in the older juveniles, with no significant differentiation for either locus a few months later in autumn. We searched for signals of haplotype structure within GH and Prl genes using next-generation amplicon deep sequencing. Both genes contained 2 groups of haplotypes, but high similarities among groups indicated that signatures of selection, if any, had largely been erased by recombination. Our results are consistent with the view that differential selection operates during early juvenile life in sea bream and highlight the importance of temporal replication in studies of post-settlement selection in marine fish. © The authors, CNRS and TOTAL Foundation 2016.},

note = {Publisher: Inter-Research},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{andree_diplectanum_2016,

title = {Diplectanum sciaenae (Van Beneden & Hesse, 1863) (Monogenea) infecting meagre, Argyrosomus regius (Asso, 1801) broodstock in Catalonia, Spain. A case report},

author = {K B Andree and A Roque and N Duncan and E Gisbert and A Estevez and M I Tsertou and P Katharios},

url = {https://doi.org/10.1016/j.vprsr.2016.02.006},

doi = {10.1016/j.vprsr.2016.02.006},

year  = {2016},

date = {2016-01-01},

journal = {Veterinary Parasitology: Regional Studies and Reports},

volume = {1-2},

pages = {75--79},

abstract = {Recurrent infection of captive meagre, Argyrosomus regius, by a monogenean parasite has been observed in the broodstock facilities of Institut de Recerca i Tecnologia Agroalimentàries (IRTA) in Catalonia, Spain, between 2008 and 2015. Following handling procedures related with hormonal treatment for spawning induction, one fish died. Post-mortem examination and detailed microscopical examination using light microscopy, SEM, and histology revealed intense infection by Diplectanum sciaenae (Monogenea, Diplectanidae) which caused noteworthy gill pathology. In the present study, we provide detailed description of the parasite and the pathology caused to its host. This is the first report associating this parasite with disease and mortality in farmed meagre.},

note = {Publisher: Elsevier B.V.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{kousteni_evidence_2016,

title = {Evidence of high genetic connectivity for the longnose spurdog Squalus blainville in the Mediterranean Sea},

author = {V Kousteni and P Kasapidis and G Kotoulas and P Megalofonou},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85019545144&doi=10.12681%2fmms.1222&partnerID=40&md5=2e93879e948e1d3748c29224910104e5},

doi = {10.12681/mms.1222},

issn = {1108393X},

year  = {2016},

date = {2016-01-01},

journal = {Mediterranean Marine Science},

volume = {17},

number = {2},

pages = {371--383},

abstract = {Squalus blainville is one of the least studied Mediterranean shark species. Despite being intensively fished in several locations, biological knowledge is limited and no genetic structure information is available. This is the first study to examine the genetic structure of S. blainville in the Mediterranean Sea. Considering the high dispersal potential inferred for other squalid sharks, the hypothesis of panmixia was tested based on a 585 bp fragment of the mitochondrial DNA cytochrome c oxidase subunit I gene from 107 individuals and six nuclear microsatellite loci from 577 individuals. Samples were collected across the Ionian, Aegean and Libyan Seas and off the Balearic Islands. Twenty three additional sequences of Mediterranean and South African origin were retrieved from GenBank and included in the mitochondrial DNA analysis. The overall haplotype diversity was high, in contrast to the low nucleotide diversity. Low and non-significant pairwise FST and FST values along with a Bayesian cluster analysis suggested high connectivity with subsequent genetic homogeneity among the populations studied, and thus a high dispersal potential for S. blainville similar to other squalids. The historical demography of the species was also assessed, revealing a pattern of population expansion since the middle Pleistocene. These findings could be considered in species-specific conservation plans, although sampling over a larger spatial scale and more genetic markers are required to fully elucidate the genetic structure and dispersal potential of S. blainville.},

note = {Publisher: Hellenic Centre for Marine Research},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{sarropoulou_sample_2016,

title = {Sample size matters in dietary gene expression studies - a case study in the gilthead sea bream (Sparus aurata L.)},

author = {Elena Sarropoulou and Styliani Adamidou and Ioannis Karacostas and Fotini Kokou},

url = {http://www.sciencedirect.com/science/article/pii/S2352513415300326},

doi = {doi:10.1016/j.aqrep.2015.12.004},

year  = {2016},

date = {2016-01-01},

journal = {Aquaculture Reports},

volume = {3},

pages = {82--87},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{christopoulou_influence_2016,

title = {Influence of hydrothermal venting on water column properties in the crater of the Kolumbo submarine volcano, Santorini volcanic field (Greece)},

author = {M E Christopoulou and T J Mertzimekis and P Nomikou and D Papanikolaou and S Carey and M Mandalakis},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84955195173&doi=10.1007%2fs00367-015-0429-z&partnerID=40&md5=0ce02912dabd658b8747026a1d7ede9a},

doi = {10.1007/s00367-015-0429-z},

issn = {02760460},

year  = {2016},

date = {2016-01-01},

journal = {Geo-Marine Letters},

volume = {36},

number = {1},

pages = {15--24},

abstract = {The Kolumbo submarine volcano, located 7 km northeast of the island of Santorini, is part of Santorini’s volcanic complex in the south Aegean Sea, Greece. Kolumbo’s last eruption was in 1650 AD. However, a unique and active hydrothermal vent field has been revealed in the northern part of its crater floor during an oceanographic survey by remotely operated vehicles (ROVs) in 2006. In the present study, conductivity-temperature-depth (CTD) data collected by ROV Hercules during three oceanographic surveys onboard E/V Nautilus in 2010 and 2011 have served to investigate the distribution of physicochemical properties in the water column, as well as their behavior directly over the hydrothermal field. Additional CTD measurements were carried out in volcanic cone 3 (VC3) along the same volcanic chain but located 3 km northeast of Kolumbo where no hydrothermal activity has been detected to date. CTD profiles exhibit pronounced anomalies directly above the active vents on Kolumbo’s crater floor. In contrast, VC3 data revealed no such anomalies, essentially resembling open-sea (background) conditions. Steep increases of temperature (e.g., from 16 to 19 °C) and conductivity near the maximum depth (504 m) inside Kolumbo’s cone show marked spatiotemporal correlation. Vertical distributions of CTD signatures suggest a strong connection to Kolumbo’s morphology, with four distinct zones identified (open sea, turbid flow, invariable state, hydrothermal vent field). Additionally, overlaying the near-seafloor temperature measurements on an X–Y coordinate grid generates a detailed 2D distribution of the hydrothermal vent field and clarifies the influence of fluid discharges in its formation. © 2015, Springer-Verlag Berlin Heidelberg.},

note = {Publisher: Springer Verlag},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{tsapakis_effects_1997,

title = {Effects of aquaculture in three coastal areas},

author = {E Tsapakis and Ioannis Karakassis and E Xatziyanni and M Maidanou},

year  = {1997},

date = {1997-01-01},

journal = {Fisheries News},

volume = {199},

pages = {90--95},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{larsson_gonadotropin-releasing_1997,

title = {Gonadotropin-releasing hormone analogue (GnRH-A) induces multiple ovulations of high-quality eggs in a cold-water, batch-spawning teleost, the yellowtail flounder (Pleuronectes ferrugineus)},

author = {D G J Larsson and C C Mylonas and Y Zohar and L W Crim},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0031449685&doi=10.1139%2fcjfas-54-9-1957&partnerID=40&md5=52dbd73406a33867ec5f010c83f98ca6},

doi = {10.1139/cjfas-54-9-1957},

issn = {0706652X},

year  = {1997},

date = {1997-01-01},

journal = {Canadian Journal of Fisheries and Aquatic Sciences},

volume = {54},

number = {9},

pages = {1957--1964},

abstract = {Sustained delivery systems for a gonadotropin-releasing hormone analogue (GnRH-A) were used for the first time in a cold-ocean teleost for inducing multiple ovulations. Intramuscular implants of 75-224 μg/kg GnRH-A ((D-Ala6,Pro9-NHEt)LHRH) were administered to female yellowtail flounder (Pleuronectes ferrugineus) either as a cholesterol pellet or as biodegradable microspheres. Females implanted with GnRH-A ovulated 3-4 weeks earlier than controls, producing multiple batches of high-quality eggs (average rates of fertilization 66-68% and hatching 55-60%); control fish eggs had significantly lower fertilization (39%) and hatching rates (25%). GnRH-A treatment doubled the egg production, synchronized the females, and shortened the interovulatory periods. The proportion of round, clear, and floating eggs lacking a perivitelline space before fertilization was an indicator of the fertilization (r2= 0.60) and hatching (r2 = 0.56) success. Moreover, egg collections of less than 10 mL varied greatly in quality, whereas larger batches, generally promoted by GnRH-A, consisted of relatively high-quality eggs. GnRH-A treated fish had increased plasma levels of estradiol- 17β 4 days after implantation. A model for prediction of the time between hormone treatment and first spawning (T; days) was developed using the initial follicle diameter (D; μm) as the only predictor (T = 365(D - 380)-082; r2 = 0.91).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{volckaert_has_1997,

title = {Has time come for the genetic management of sea bass?},

author = {F Volckaert and M C Alvarez and F Argenton and F Bonhomme and B Chatain and L Colombo and R Castilho and B Chevassus and G Gorshkova and M Kohler and A Magoulas and G Martinez and B McAndrew and F Piferrer and M Vandeputte and S Zanuy},

year  = {1997},

date = {1997-01-01},

journal = {Aquaculture Europe},

volume = {22},

number = {2},

pages = {46--47},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{mylonas_hormonal_1997,

title = {Hormonal changes in male white bass (Morone chrysops) and evaluation of milt quality after treatment with a sustained-release GnRHa delivery system},

author = {C C Mylonas and A Gissis and Y Magnus and Y Zohar},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030759556&doi=10.1016%2fS0044-8486%2897%2987566-7&partnerID=40&md5=46bd2d2d502a25d0f3ec6e54a305aba9},

doi = {10.1016/S0044-8486(97)87566-7},

issn = {00448486},

year  = {1997},

date = {1997-01-01},

journal = {Aquaculture},

volume = {153},

number = {3-4},

pages = {301--311},

abstract = {Captive white bass, (Morone chrysops) were exposed to an increase in temperature and treated with a gonadotropin-releasing hormone agonist (GnRHa) delivery system during the spermiation period. Circulating levels of various reproductive hormones, and milt production and quality were examined before and after GnRHa treatment. Circulating levels of gonadotropin II (GtH II) prior to GnRHa treatment were below the assay detection limit (0.4 ng ml-1), while mean (±SEM) plasma levels of 17α,20β-dihydroxy-4-pregnen-3-one (17,20β-P) were 0.09 ± 0.03 ng ml-1 testosterone (T) levels were 0,79 ± 0.11 ng ml-1 and 11-ketotestosterone (11-KT) levels were 1.7 ± 0.3 ng ml-1. Treatment with the GnRHa delivery system induced significant increases in plasma GtH 11 and 17,20β-P after 7 days, whereas T and 11-KT were unaffected. Plasma 17α,20β,21-trihydroxy-4-pregnen-3-one (17,20β,21-P) remained unchanged during the experiment with a mean of 0.55 ± 0.05 ng ml-1. Mean total expressible milt increased significantly after treatment with the GnRHa delivery system, from 2.0 ± 0.1 ml kg-1 body weight on day 0 to 3.6 ± 0.5 ml kg-1 on day 7. Milt production remained constant in saline-injected control fish and averaged 2.1 ± 0.2 ml kg-1. Sperm density, motility and fertilization percentage of total inseminated eggs did not differ between milt from GnRHa- and saline-injected fish, and averaged 52 ± 2 x 109 spermatozoa ml-1, 50 ± 4% motile spermatozoa and 60 ± 5% fertilized eggs, respectively. These results demonstrate the effectiveness of sustained GnRHa treatment in enhancing mill production in white bass, without negatively affecting mill quality. It is assumed that enhancement of milt production is caused by high circulating GtH II levels, via the action of 17.20β-P.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{borsa_infraspecific_1997-1,

title = {Infraspecific zoogeography of the Mediterranean: population genetic analysis on sixteen Atlanto-Mediterranean species (fishes and invertebrates)},

author = {P Borsa and M Naciri and L Bahri and L Chikhi and F J Garcia de Leon and G Kotoulas and F Bonhomme},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0031403021&partnerID=40&md5=84cd00d266e65a028ccbf3c537e40b88},

year  = {1997},

date = {1997-01-01},

journal = {Vie et Milieu},

volume = {47},

pages = {295 -- 305},

abstract = {The faunal relationships of the Atlantic and the Mediterranean have been affected by tectonic change in the Miocene, by climate change in the Pleistocene and by hydrological discontinuity. We use a population genetics approach to the biogeography of the Mediterranean marine fauna, in particular to describe the level of recent or present-day exchanges with the Atlantic. The level of genetic differences between populations was estimated from published and unpublished data on sixteen species from the northeastern Atlantic and the Mediterranean. For a majority of species, either tropical or subtropical or boreal fishes, or coastal invertebrates, a moderately strong to strong genetic cline between each side of the Gibraltar strait area was observed. Such a pattern was also observed in a proportion of species between each side of the Sicilian-Tunisian strait. In a limited number of cases, the geographic patterns of population genetic structure however conformed with the expectations from an isolation-by-distance model. The molecular phylogeographic approach in a boreal species, the flounder Platichthys flesus, led to the inferrence of the historical movements of colonisation and geographic isolation of the populations. A similar approach used in a tropical species (the Spanish sardine Sardinella aurita) and a cosmopolitan species (the grey mullet Mugil cephalus) revealed close genetic relationships between Mediterranean and west-tropical Atlantic populations. We analyse the possible implications of these findings to the systematics of the Mediterranean populations and we speculate about the future of boreal species vis-a-vis the present climatic change. We debate on various hypotheses for the maintaining of abrupt genetic clines in the marine environment.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{borsa_infraspecific_1997,

title = {Infraspecific zoogeography of the Mediterranean: population genetic analysis on sixteen Atlanto-Mediterranean species (fishes and invertebrates) [Zoogeographie infra-specifique de la Mer Mediterranee: analyse des donnees genetiques populationnelles sur seize especes atlanto-mediterraneennes (poissons et invertebres)]},

author = {P Borsa and M Naciri and L Bahri and L Chikhi and F J Garcia de Leon and G Kotoulas and F Bonhomme},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0031403021&partnerID=40&md5=84cd00d266e65a028ccbf3c537e40b88},

issn = {02408759},

year  = {1997},

date = {1997-01-01},

journal = {Vie et Milieu},

volume = {47},

number = {4},

pages = {295--305},

abstract = {The faunal relationships of the Atlantic and the Mediterranean have been affected by tectonic change in the Miocene, by climate change in the Pleistocene and by hydrological discontinuity. We use a population genetics approach to the biogeography of the Mediterranean marine fauna, in particular to describe the level of recent or present-day exchanges with the Atlantic. The level of genetic differences between populations was estimated from published and unpublished data on sixteen species from the northeastern Atlantic and the Mediterranean. For a majority of species, either tropical or subtropical or boreal fishes, or coastal invertebrates, a moderately strong to strong genetic cline between each side of the Gibraltar strait area was observed. Such a pattern was also observed in a proportion of species between each side of the Sicilian-Tunisian strait. In a limited number of cases, the geographic patterns of population genetic structure however conformed with the expectations from an isolation-by-distance model. The molecular phylogeographic approach in a boreal species, the flounder Platichthys flesus, led to the inferrence of the historical movements of colonisation and geographic isolation of the populations. A similar approach used in a tropical species (the Spanish sardine Sardinella aurita) and a cosmopolitan species (the grey mullet Mugil cephalus) revealed close genetic relationships between Mediterranean and west-tropical Atlantic populations. We analyse the possible implications of these findings to the systematics of the Mediterranean populations and we speculate about the future of boreal species vis-a-vis the present climatic change. We debate on various hypotheses for the maintaining of abrupt genetic clines in the marine environment.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{karakassis_interaction_1997,

title = {Interaction of Aquaculture and the Marine Environment. Methodology and preliminary results},

author = {Ioannis Karakassis and E Tsapakis and E Xatziyanni and M Maidanou and K -N Papadopoulou and P Pita and C Smith},

year  = {1997},

date = {1997-01-01},

journal = {Fisheries News},

volume = {192},

pages = {40--45},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{schulz_modulation_1997,

title = {Modulation of testicular androgen production in adolescent African catfish (Clarias gariepinus)},

author = {R W Schulz and F Van Der Wind and C Janssen-Dommerholt and J Peute and C C Mylonas and Y Zohar and P Swanson and H J T Goos},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0031260440&doi=10.1006%2fgcen.1997.6951&partnerID=40&md5=13ca91398eaf6fdeffb0cdab1a93756c},

doi = {10.1006/gcen.1997.6951},

issn = {00166480},

year  = {1997},

date = {1997-01-01},

journal = {General and Comparative Endocrinology},

volume = {108},

number = {1},

pages = {56--66},

abstract = {At 6 months of age the first spermatozoa appear in the testes of the African catfish considered to be adolescent, since the development to adulthood (12 months of age) is accompanied by further morphological and functional differentiation of Leydig cells. There are increasing plasma levels of 11-ketotestosterone (11-KT) and an increasing responsiveness to luteinizing hormone (LH) of testicular androgen secretion in vitro. Whether treatment of adolescent males with key hormones of the brain-pituitary-gonad axis [gonadotropin-releasing hormone (GnRH), LH, and 11-KT] affects the testicular steroidogenic response to a challenge with LH in vitro 7 days later has been investigated. Injection of GnRH (2.5 μg chicken GnRH-II per kilogram of body weight), LH (25 μg/kg), or a high dose of 11-KT (50 μg/kg) down-regulated basal and LH-stimulated testicular androgen secretion to a minimum of 35% of control values. Treatment with LH was, moreover, associated with changes in the ultrastructure of Leydig cell mitochondria which were either swollen and had a less electron-dense matrix or showed an elongated shape. Conversely, a moderate dose of 11-KT (20 μg/kg) enhanced LH- stimulated, but not basal, androgen secretion in vitro to a maximum of 190% of control values. In view of the generally low LH plasma levels and of the steadily increasing 11-KT plasma levels during puberty, 11-KT may be involved in the up-regulation of the testicular steroidogenic capacity observed during development to full maturity.},

note = {Publisher: Academic Press Inc.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{alexis_pathological_1997,

title = {Pathological findings owing to the lack of ascorbic acid in cultured gilthead bream (Sparus aurata L.)},

author = {M N Alexis and K K Karanikolas and R H Richards},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0030744502&partnerID=40&md5=a2139d0a7cbd31d6263725d17ad392b7},

year  = {1997},

date = {1997-01-01},

journal = {Aquaculture},

volume = {151},

pages = {209 -- 218},

abstract = {The appearance of pathological signs owing to vitamin C deficiency and the wound healing response were followed in experiments with gilthead bream, initial weight 0.5 g, fed with increasing levels of ascorbic acid in the diets. The levels of ascorbic acid used ranged from 0 to 3,2 g kg-1 diet. Certain pathological signs such as extensive tubular damage, glomerulonephritis, and inflammatory response of the haemopoetic tissue producing granuloma and in some cases extending in the nearby tissues and organs, appeared in all fish fed the vitamin C deficient diet, while fish fed the diet supplemented with 50 mg ascorbate per kilogram of diet showed only damage to renal tubules. The gross deficiency signs observed were anorexia, scale loss, depigmentation, internal and external haemorrhages. Mortalities reached a high level after the first month of rearing. The wound healing response showed a direct correlation to ascorbate level in the diet. The unknown etiology of granulomatosis observed by other researchers in cultured seabream may be related to vitamin C deficiency, since in this experiment, it produced the same kidney pathology.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pitta_planktonic_1997,

title = {Planktonic food web in marine mesocosms in the Eastern Mediterranean: Bottom-up or top-down regulation?},

author = {P Pitta and A Giannakourou and P Divanach and M Kentouri},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0031402824&partnerID=40&md5=d32feeb1ddf43866aab11765538d6bfa},

year  = {1997},

date = {1997-01-01},

journal = {Hydrobiologia},

volume = {363},

pages = {97 -- 105},

abstract = {A mesocosm experiment was conducted in order to study the structure of the planktonic food web. The dynamics of pico-, nano- and microplankton populations were followed during 40 days in four large (40 m3) enclosures. In three tanks a gradient of added nutrients (nitrogen and phosphorus) was applied, while a fourth tank was used as a control. On day 14, the top predator (sea bream Sparus aurata larvae) was introduced into the tanks and part of the water column in each tank was isolated in a plastic bag without fish larvae, to act as a control for predation. Physical parameters, chlorophyll a and nutrient concentrations, as well as plankton concentrations were monitored. A diatom bloom was observed in all four tanks, in the first phase ending with silicate depletion. Flagellate and dinoflagellate abundance subsequently increased, these organisms being limited by zooplankton grazing. The zooplankton populations were controlled by both resources (mostly flagellates) and predation (by fish larvae) as indicated by the results of the control experiments.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{mylonas_plasma_1997,

title = {Plasma gonadotropin II, sex steroids, and thyroid hormones in wild striped bass (Morone saxatilis) during spermiation and final oocyte maturation},

author = {C C Mylonas and A P Scott and Y Zohar},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030728062&doi=10.1006%2fgcen.1997.6967&partnerID=40&md5=e2e788f3e08d9b279b44c1e17b96812f},

doi = {10.1006/gcen.1997.6967},

issn = {00166480},

year  = {1997},

date = {1997-01-01},

journal = {General and Comparative Endocrinology},

volume = {108},

number = {2},

pages = {223--236},

abstract = {The blood levels of gonadotropin II (GtH II), sex-steroid hormones, and thyroid hormones were determined in wild spermiating male striped bass (Morone saxatilis) in males and in females at various stages of final oocyte maturation (FOM), captured on their spawning grounds. The progression of spermiation was associated with increases in plasma GtH II and decreases in plasma testosterone (T), 11-ketotestosterone, and thyroxine (T4). Plasma triiodothyronine (T3) remained at high and relatively unchanged levels. Plasma levels of 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P) and 17,20β,21-trihydroxy-4-pregnen-3-one (17,20β,21-P), the proposed maturation-inducing steroids (MIS) in striped bass, were low and unchanged during the same period. It was concluded that low progestogen levels are adequate to induce spermiation in striped bass, and that higher levels may be associated with spawning behavior. In the females, based on the profiles of the studied hormones, FOM was separated into two phases. Early FOM, which included germinal vesicle (GV) migration and lipid-droplet coalescence, was associated with elevations in plasma GtH II, T, and estradiol 17β. Late FOM, which included GV breakdown and yolk-globule coalescence, was associated with a further surge in plasma GtH II, increases in the levels of the two MIS, mainly 17,20β-P, and a drop in T4. Plasma T3 levels did not change during FOM. Examination of conjugated steroids demonstrated, in the males, a reduction in conjugated androgens at the peak of the spawning season and, in the females, a small increase in conjugated 17,20β-dihydroxylated and 5β- reduced, 3α-hydroxylated steroids after spawning. This is the most comprehensive report, to date, on the endocrine regulation of gonadal maturation in wild striped bass, demonstrating that a two-stage process of FOM is regulated by different endocrine signals, providing further evidence for the involvement of 17,20β-P as a MIS in the females, and indicating that both males and females are in an euthyroid state during the spawning season.},

note = {Publisher: Academic Press Inc.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{tsagkarakou_population_1997,

title = {Population structure in the spider mite Tetranychus urticae (Acari: Tetranychidae) from Crete based on multiple allozymes},

author = {Anastasia Tsagkarakou and Maria Navajas and Jacques Lagnel and Nicole Pasteur},

url = {http://www.nature.com/articles/hdy199710},

doi = {10.1038/hdy.1997.10},

issn = {0018-067X, 1365-2540},

year  = {1997},

date = {1997-01-01},

urldate = {2020-08-18},

journal = {Heredity},

volume = {78},

number = {1},

pages = {84--92},

abstract = {The polymorphism of four isozymes was studied on single females of Tetranychus urticae from Crete (Greece), using an isoelectric focusing technique. Genetic differentiation was found to be correlated with distance but not with the species of colonized host-plants. Thus no differentiation was observed between samples collected on citrus trees, tomato, pumpkin, okra or weed plants located within a 50 m2 area, showing that at this geographical scale T. urticae, populations are panmictic. In contrast, samples from plants at 150 m or more from one another displayed a significant genetic differentiation. These results are discussed in relation to the known pattern of migration in the species.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{mylonas_reproductive_1997,

title = {Reproductive biology and endocrine regulation of final oocyte maturation of captive white bass},

author = {C C Mylonas and Y Magnus and Y Klebanov and A Gissis and Y Zohar},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030741032&doi=10.1006%2fjfbi.1997.0433&partnerID=40&md5=aa2b0d7a5082efd22a1935123608e83a},

doi = {10.1006/jfbi.1997.0433},

issn = {00221112},

year  = {1997},

date = {1997-01-01},

journal = {Journal of Fish Biology},

volume = {51},

number = {2},

pages = {234--250},

abstract = {The ovarian development, and plasma levels of gonadotropin II (GtH II) and sex-steroid hormones at the end of vitellogenesis were examined in captive white bass Morone chrysops. The changes in plasma hormone levels and oocyte morphology associated with gonadotropin-releasing hormone agonist (GnRHa)-induced final oocyte maturation (FOM) were studied. Although plasma 17β-oestradiol (E2) and oocyte diameter increased, there were no changes in GtH II, testosterone (T), 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P) or 17,20β,21-dihydroxy-4-pregnen-3-one (17,20β,21-P) in non-hormone-treated females, and no FOM was observed. Treatment with a sustained-release GnRHa delivery system (GnRHa implant) induced two FOM cycles separated by about 24 h, with the release of approximately equal numbers of eggs in each spawn. Plasma GtH II levels were elevated significantly throughout FOM, reaching a maximum of 9.07 ± 1.55 ng ml-1 in ovulated fish. Both plasma E2 and T increased soon after the GnRHa treatment, but E2 declined in fish undergoing germinal vesicle (GV) migration. Plasma T increased further during FOM (7.55 ± 2.87 ng ml-1), but declined precipitously at ovulation. A surge in plasma 17,20β-P and 17,20β,21-P (4.11 ± 0.97 ng ml-1 and 3.10 ± 0.77 ng ml-1, respectively) was observed in females undergoing GV breakdown (GVBD). Based on the involvement of different sex-steroid hormones, FOM was separated into two stages. Early FOM included lipid-droplet coalescence and GV migration, and was associated with elevations in plasma GtH II and T. Late FOM included GVBD and yolk-globule coalescence, and was associated with elevations in plasma GtH II, 17,20β-P and 17,20β,21-P. The results of this study point to the absence of a surge in plasma GtH II as the missing link in the reproductive axis responsible for the failure of captive white bass to undergo FOM at the end of vitellogenesis. Sustained elevation of plasma GtH II via treatment with a GnRHa implant induced two consecutive spawns with an overall egg production two- to eightfold higher than previously obtained from captive broodstocks, and similar to annual egg production values reported for wild fish.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{chatzifotis_effect_1997,

title = {The Effect of Dietary Carnitine Supplementation on Growth of Rainbow Trout Fingerlings},

author = {S Chatzifotis and T Takeuchi and T Watanabe and S Satoh},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0000426025&doi=10.2331%2ffishsci.63.321&partnerID=40&md5=eaacc3243620e4ec5c812ae3c67906b5},

doi = {10.2331/fishsci.63.321},

issn = {09199268},

year  = {1997},

date = {1997-01-01},

journal = {Fisheries Science},

volume = {63},

number = {2},

pages = {321--322},

note = {Publisher: Blackwell Publishing},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{arvanitidis_genus_1997,

title = {The genus Paradiopatra (Polychaeta, Onuphidae) in the Mediterranean with the description of Paradiopatra calliopae sp. nov.},

author = {C Arvanitidis and A Koukouras},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030893558&doi=10.1080%2f00785326.1997.10432477&partnerID=40&md5=493ee746ab970481fdc7617b2e1a3eba},

doi = {10.1080/00785326.1997.10432477},

issn = {00785326},

year  = {1997},

date = {1997-01-01},

journal = {Ophelia},

volume = {46},

number = {1},

pages = {51--63},

abstract = {Sampling in circalittoral and bathyal zones of the North Aegean Sea, yielded a considerable number of specimens belonging to two species of the genus Paradiopatra Ehlers, 1887: P. quadricuspis (M. Sars, 1872) and P. calliopae sp. nov. P. quadricuspis from the North Aegean show slight differences from the type material. In addition, some features of the species, not reported by previous authors, are given. Also, the holotype of the Atlantic species P. bihanica (Intes & Le Loeuff, 1975) was re-examined and useful conclusions about the status of this species and its distinction from the new species are drawn. Re-examination of a specimen from the Ionian Sea, reported as Onuphis lepta, shows that it possibly belongs to the new species. The new species is compared with its closest congeners (P. quadricuspis, P. bihanica, and P. fauchaldi (Kirkegaard, 1988)) and the characters distinguishing them are given. The status of the genus in the Mediterranean is discussed. © 1997 Taylor & Francis Group, LLC.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{mylonas_application_1996,

title = {Application of controlled-release, GnRHa-delivery systems in commercial production of white bass X striped bass hybrids (sunshine bass), using captive broodstocks},

author = {C C Mylonas and Y Magnus and A Gissis and Y Klebanov and Y Zohar},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0000911285&doi=10.1016%2f0044-8486%2895%2901178-1&partnerID=40&md5=5c8c94d7c52f9d763d1866eec84ca115},

doi = {10.1016/0044-8486(95)01178-1},

issn = {00448486},

year  = {1996},

date = {1996-01-01},

journal = {Aquaculture},

volume = {140},

number = {3},

pages = {265--280},

abstract = {Hatchery-produced white bass (Morone chrysops) and striped bass (M. saxatilis) reared to maturity in a commercial aquaculture facility, were successfully spawned using controlled-release delivery systems containing the gonadotropin-releasing hormone analog DAla6, Pro9[NEt]-GnRH (GnRHa). Two-year-old white bass females (mean weight, 0.81 kg) were implanted with different polymer-based, GnRHa delivery systems at doses ranging from 40 to 89 μg GnRHa kg-1 body weight. GnRHa treatment on 20 February 1994, when females contained oocytes up to 720 μm in diameter, induced ovulation of all fish between 35 to 82 h after treatment. The white bass eggs produced were fertilized with sperm from striped bass for the production of sunshine bass. An average of 294500 eggs kg-1 were produced, with a mean fertility of 81.2%, 24 h survival of 46.5%, and overall hatching success of 45%. Survival from hatch to 30 days post-hatch was 78% and the fry weighed between 0.07 and 0.1 g. Overripening of eggs began within 1 h from ovulation and maximum fertilization (60%) was observed when eggs were stripped 0.5 h after ovulation. Fertilization success decreased thereafter to 31% and 10% by 1 h and 3 h after ovulation, respectively. Control fish not treated with GnRHa did not show any signs of final oocyte maturation during the period of the study. GnRHa administration via controlled-release delivery systems appears to be a very effective method for inducing high fecundity ovulation of captive white bass broodstocks, and producing eggs of high fertility and hatching success.},

note = {Publisher: Elsevier},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{karakassis_aquaculture_1996,

title = {Aquaculture & Environment: a research project of the 2nd Operational Program of Greece, General Secretariat of Research & Technology},

author = {Ioannis Karakassis and C Smith and K -N Papadopoulou and E Tsapakis and E Xatziyanni and M Maidanou and A Frentzos and P White and S Kourakis and S Katranidis},

year  = {1996},

date = {1996-01-01},

journal = {Fisheries News},

volume = {178},

pages = {36--41},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{boujard_diel_1996,

title = {Diel feeding activity and the effect of time-restricted self-feeding on growth and feed conversion in European sea bass},

author = {T Boujard and M Jourdan and M Kentouri and P Divanach},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0029667241&partnerID=40&md5=4f928f95721eadc7b9602a9134a4da65},

year  = {1996},

date = {1996-01-01},

journal = {Aquaculture},

volume = {139},

pages = {117 -- 127},

abstract = {The feeding rhythm and growth performance of European sea bass Dicentrarchus labrax (initial weight 65 g ) were studied under free and time-restricted access to self-feeders. Under free access to the feeders, significant feeding rhythms with a nocturnal acrophase were observed, although there was considerable variability between replicates. When access to the self-feeders was restricted to two periods of 4 h day-1, the feed demand (FD) was reduced at the beginning of the experiment but not at the end, indicating that the period of adaptation, rather than appetite, was affected by such feeding practice. When access to the self-feeders was restricted to only one period of 4 h day-1, two thirds of the replicates eventually displayed FD similar to the fish having free access to feed, indicating that the restricted temporal access to feed could be compensated for. There was no detectable effect of feeding protocol on feed conversion or body composition, but growth was closely related to the total FD. Thus, growth was influenced by the fact that fish subjected to a time-restricted access to feed needed time to adapt to the new feeding situation. The results indicate that sea bass are capable of operating self-feeders, and can easily modify their feeding, resulting in both good growth performance and good feed conversion. © 1996 Elsevier Science B.V. All rights reserved.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{machera_fenbutatin_1996,

title = {Fenbutatin acute toxicity on Artemia nauplii: Effects of sublethal concentrations on ATPase activity},

author = {K Machera and E Cotou and P Anastassiadou},

url = {http://link.springer.com/10.1007/s001289900023},

doi = {10.1007/s001289900023},

issn = {0007-4861, 1432-0800},

year  = {1996},

date = {1996-01-01},

urldate = {2020-08-18},

journal = {Bulletin of Environmental Contamination and Toxicology},

volume = {56},

number = {1},

pages = {159--164},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{tsagkarakou_genetic_1996,

title = {Genetic variability in Tetranychus urticae (Acari: Tetranychidae) from Greece: Insecticide resistance and isozymes},

author = {A Tsagkarakou and M Navajas and J Lagnel and J Gutierrez and N Pasteur},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0030485243&partnerID=40&md5=5ca68d1453772b90fd6e7deb150f7fc2},

year  = {1996},

date = {1996-01-01},

journal = {Journal of Economic Entomology},

volume = {89},

pages = {1354 -- 1358},

abstract = {Resistance to methyl-parathion methidathion, and methomyl was studied by bioassays in Tetranychus urticae Koch from Greece. At LC50, the resistance ratio was highly variable with methidathion (5- to 63-fold) and with methomyl (6- to 34-fold). The mortality curves with methyl-parathion showed a clear plateau at ≃20% mortality, and the resistance ratio was ≃50 fold at LC50 in all samples. By using isoelectric focusing on cellulose acetate membranes, electrophoretic studies of 5 loci encoding esterases (Est-1 and Est-2), glucose-phosphate isomerase (Gpi), malic enzyme (Me), and phosphoglucomutase (Pgm) disclosed large genetic differences among the samples.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{koukouras_macrofauna_1996,

title = {Macrofauna associated with sponge species of different morphology},

author = {A Koukouras and A Russo and E Voultsiadou-Koukoura and C Arvanitidis and D Stefanidou},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030427632&doi=10.1111%2fj.1439-0485.1996.tb00418.x&partnerID=40&md5=ab85ea94afe5115359c48dad5fa7b5fb},

doi = {10.1111/j.1439-0485.1996.tb00418.x},

issn = {01739565},

year  = {1996},

date = {1996-01-01},

journal = {Marine Ecology},

volume = {17},

number = {4},

pages = {569--582},

abstract = {The macrofaunas associated with the three morphologically different North Aegean Sea sponge species Agelas oroides, Aplysina aerophoba, and Axinella cannabina were compared. The faunal abundance was not related to sponge surface area for any of the three species. Each sponge species supported a significantly different number of individuals per unit canal volume; A. aerophoba supported the most, A. oroides the least. A. aerophoba supported the highest species richness, A. cannabina the lowest. Canal volume was a good predictor of associated faunal abundance and species richness for two of the three sponge species. Faunal abundance was also directly related to sponge mean canal diameter. The species composition of associated fauna was similar on A. aerophoba and A, oroides and significantly different on A. cannabina.},

note = {Publisher: Blackwell Verlag GmbH Berlin},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{navajas_mitochondrial_1996,

title = {Mitochondrial COI sequences in mites: Evidence for variations in base composition},

author = {M Navajas and D Fournier and J Lagnel and J Gutlerrez and P Boursot},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0030293916&partnerID=40&md5=4f94d775771f686219da59d73f30bdc4},

year  = {1996},

date = {1996-01-01},

journal = {Insect Molecular Biology},

volume = {5},

pages = {281 -- 285},

abstract = {Studies of mitochondrial DNA sequences in a variety of animals have shown important differences between phyla, including differences in the genetic codes used, and varying constraints on base composition. In that respect, little is known of mites, an important and diversified group. We sequenced a portion (340 nt) of the cytochrome oxidase subunit I (COI) encoding gene in twenty species of phytophagous mites belonging to nine genera of the two families Tetranychidae and Tenuipalpidae. The mitochondrial genetic code used in mites appeared to be the same as in insects. As is generally also the case in insects, the mite sequences were very rich in A + T (75% on average), especially at the third codon position (94%). However, important variations of base composition were observed among mite species, one of them showing as little as 69% A+T. Variations of base composition occur mostly through synonymous transitions, and do not have detectable effects on polypeptide evolution in this group.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{navajas_mitochondrial_1996-1,

title = {Mitochondrial cytochrome oxidase I in tetranychid mites: A comparison between molecular phylogeny and changes of morphological and life history traits},

author = {M Navajas and J Gutierrez and J Lagnel and P Boursot},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0000562466&partnerID=40&md5=b1b86a08dca24bd4ec61e645c29138b7},

year  = {1996},

date = {1996-01-01},

journal = {Bulletin of Entomological Research},

volume = {86},

pages = {407 -- 417},

abstract = {Spider mites, Tetranychidae, represent one of the most cosmopolitan and economically important groups of terrestrial arthropods; however, many aspects of their evolutionary relationships remain uncertain. We sequenced part of the mitochondrial cytochrome oxidase subunit I (COI) gene in 20 species of phytophagous mites belonging to nine genera and two families (Tetranychidae and Tenuipalpidae), including several agricultural pests. As reported in insects, the sequences were extremely rich in A+T (75% on average), especially in the third codon position (95%). However, one of the genera we studied had a significantly lower A+T content (69% on average, 78% in the third codon position), showing that base composition can change substantially over short periods of time. Most interspecific differences were transversions and their number increased steadily with the number of non-synonymous differences, while the number of transitions remained constant. The phylogeny based on COI sequences was inferred using the maximum likelihood method. The results are compatible as a whole with the traditional classification based on morphological characters, but call for some minor taxonomic revisions. Some morphological characters and life history traits (mode of reproduction, adaptation to the host plant) were also analysed within this phylogenetic framework. At the family level, one can see a trend towards thelytoky becoming rarer compared to the general mode of reproduction of the group, arrhenotoky. There is also an evolutionary tendency towards a more complex mode of life, with the production of silk webs and correlated changes of the locomotion apparatus. However, in the Tetranychidae there seems to have been convergent evolution of these morphological characters together with independent development of a common adaptation to this mode of life in different genera.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{magoulas_mitochondrial_1996,

title = {Mitochondrial DNA phylogeny and the reconstruction of the population history of a species: the case of the European anchovy (Engraulis encrasicolus)},

author = {A Magoulas and N Tsimenides and E Zouros},

url = {https://academic.oup.com/mbe/article-lookup/doi/10.1093/oxfordjournals.molbev.a025554},

doi = {10.1093/oxfordjournals.molbev.a025554},

issn = {0737-4038, 1537-1719},

year  = {1996},

date = {1996-01-01},

urldate = {2020-08-09},

journal = {Molecular Biology and Evolution},

volume = {13},

number = {1},

pages = {178--190},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{kasapidis_neue_1996,

title = {Neue Daten über die Herpetofauna von Lesbos (ägäische Inseln, Griechenland) und einige biogeographische Bemerkungen über die Inseln des nordöstlichen ägäischen Archipels},

author = {P Kasapidis and S Provatidou and P Maragou and E D Valakos},

year  = {1996},

date = {1996-01-01},

journal = {Salamandra},

volume = {32},

number = {3},

pages = {171--180},

abstract = {New data on the herpetofauna of Lesbos ( Aegean Islands, Greece) and some biogeographical remarks on the islands of the northern Aegean Archipelago. Fourteen species of reptiles were found on Lesbos lsland. The species Ablepharus kitaibelii and Mallolon monspessulanus are recorded for the first time from the island. The localities and some morphometric characters of the caught specimens are noted. Finally, we attempt to combine our data and the bibliographical ones in a biogeographical approach to the fauna of the islands of the northeastern Aegean Archipelago.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nengas_partial_1996,

title = {Partial substitution of fishmeal with soybean meal products and derivatives in diets for the gilthead sea bream Sparus aurata (L.)},

author = {I Nengas and M N Alexis and S J Davies},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0001414634&partnerID=40&md5=3d8f337f2bc02e7328233a341eeacc51},

year  = {1996},

date = {1996-01-01},

journal = {Aquaculture Research},

volume = {27},

pages = {147 -- 156},

abstract = {Two growth trials were conducted to evaluate the nutritional quality of several soybean products as constituents in diets for the gilthead sea bream. Sparus aurata (L). In a preliminary experiment, the fish were fed six diets containing different levels of solvent extracted soybean meal as a replacement for white fishmeal at four substitution levels: 10, 20, 30 and 40% of the fishmeal protein component. The diets supported less growth as the inclusion of soybean meal increased. However, significant reductions in growth were apparent at the 30% substitution level. All growth parameters followed the same trend. In the second experiment, six diets with 35% of the total protein contributed from differently processed soybean meals were tested. The products included three industrial full-fat meals heat processed for different periods, a solvent extracted meal and a soya protein concentrate. Protein digestibility coefficients were measured for all the experimental diets. All growth parameters of the fish fed the underheated full-fat meal, solvent extracted meal and soya concentrate were significantly lower than the control group. Protein digestibility coefficients were similar with no statistical differences (P textless 0.05).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{tsigenopoulos_phylogenetic_1996,

title = {Phylogenetic relationships of Leuciscus keadicus, an endemic cyprinid species from Greece, with other species of the genus Leuciscus},

author = {C S Tsigenopoulos and Y Karakousis},

year  = {1996},

date = {1996-01-01},

journal = {Folia Zoologica},

volume = {45},

pages = {87--93},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{holland_sperm_1996,

title = {Sperm characteristics of precocious 1-year-old male striped bass Morone saxatilis},

author = {M C Holland and C C Mylonas and Y Zohar},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030542074&partnerID=40&md5=491dca9c066c1dfe3c2c338578e299b6},

issn = {08938849},

year  = {1996},

date = {1996-01-01},

journal = {Journal of the World Aquaculture Society},

volume = {27},

number = {2},

pages = {208--212},

abstract = {Captive-reared, 10-mo-old, male striped bass Marone saxatilis were sampled monthly for testicular development between February and June 1994. One of the five males sampled in February showed precocious testicular development and had a gonadosomatic index (GSI) of 1.26%. while the other four fish had immature testes with a mean GSI ± Sx of 0.17 ± 0.03%. Spermiating individuals were present from April to June. In April the average body weight (BW) of spermiating males was 65 ± 4 g and their GSI reached a mean value of 4.75 ± 0.52%. In June, milt collected from ten precocious males contained motile spermatozoa with a mean of 31 ± 7% of the sperm showing forward movement. Mean milt volume and sperm concentration were 1.67 ± 0.41 mL/kg BW and 92.3 ± 1.8 x 109 spermatozoa/mL, respectively. These data show that male striped bass reared in captivity can reach sexual maturity during their first year. This is one year earlier than previously reported for striped bass in mid-Atlantic regions.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{sorbera_sustained_1996,

title = {Sustained administration of GnRHa increases milt volume without altering sperm counts in the sea bass},

author = {L A Sorbera and C C Mylonas and S Zanuy and M Carrillo and Y Zohar},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030443470&doi=10.1002%2f%28SICI%291097-010X%2819961201%29276%3a5%3c361%3a%3aAID-JEZ6%3e3.0.CO%3b2-M&partnerID=40&md5=da145966d5c694db401ff311d820caab},

doi = {10.1002/(SICI)1097-010X(19961201)276:5<361::AID-JEZ6>3.0.CO;2-M},

issn = {0022104X},

year  = {1996},

date = {1996-01-01},

journal = {Journal of Experimental Zoology},

volume = {276},

number = {5},

pages = {361--368},

abstract = {The role of gonadotropin-releasing hormone (GnRH) in spermiogenesis and spermiation is not well understood. This study examined the effects of different modes of administration of a GnRH analog (GnRHa; [D-Ala6, Pro9NEt]-mGnRH) on spermiation in the sea bass, Dicentrarchus labrax. Groups of sea bass received either GnRHa injection in saline (I; 25 μg/kg body weight [BW] or one of three types of GnRHa sustained release polymeric device: a fast-releasing implant (ethylene-vinyl acetate copolymer [EVAc]; 100 μg/kg BW), a slower-releasing implant (ethylene-vinyl acetate copolymer [EVSL]; 100 μg/kg BW), or biodegradable microspheres (M; 50 μg/kg BW). Total expressible milt was collected and assessed for volume, motility, and counts at various intervals for 44 days. Untreated males produced a total of 9.4 ± 3.4 ml/kg BW (mean ± SEM) of milt over the 44-day experimental period, with milt production ending by day 28. All GnRHa treatments stimulated a significant increase in total milt volume (ml/kg BW) peaking by day 2 (I: 11.8 ± 1.8) or day 7 (EVAc: 20.0 ± 1.5; M: 26.8 ± 2.7; EVSL: 27.9 ± 4.3) posttreatment. While milt volumes in injected males returned to control values by day 14, all groups treated with sustained GnRHa delivery systems maintained significantly elevated milt volumes for 21 days (EVAc group) or 35 days (EVSL and M groups). Sperm motility was consistently good to excellent (70-100% vigorously active) in all groups when expressible milt volume was above 1.0 ml, and there were no significant differences in sperm counts (averaging 5.5 ± 0.1 x 109 spermatozoa/ml). These data show that sustained administration of GnRHa significantly increases and prolongs spermiation in the sea bass without altering sperm concentration or quality.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{chatzifotis_effect_1996,

title = {The effect of dietary carnitine supplementation on growth of red sea bream (Pagarus major) fingerlings at two levels of dietary lysine},

author = {S Chatzifotis and T Takeuchi and T Seikai},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030454803&doi=10.1016%2fS0044-8486%2896%2901403-2&partnerID=40&md5=0749d0b12ee23953ba9e2ae0e5221b48},

doi = {10.1016/S0044-8486(96)01403-2},

issn = {00448486},

year  = {1996},

date = {1996-01-01},

journal = {Aquaculture},

volume = {147},

number = {3-4},

pages = {235--248},

abstract = {The effect of dietary carnitine supplementation (2 g kg-1 diet) on growth, proximate, neutral lipid and fatty acid composition of liver and muscle of red sea bream fingerlings at two levels of dietary lysine (10 and 14 g kg-1 diet, respectively) was investigated. Carnitine increased red sea bream growth fed a 14 g lysine kg-1 diet (P < 0.05) but did not cause any effect on growth in fish fed the 10 g lysine kg-1) diet. A clear lysine sparing effect of carnitine in red sea bream was not observed. Carnitine did not reduce crude lipid levels in white muscle and liver of fish. Fatty acid composition of total lipids in liver showed reduced values for eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and total long-chain fatty acids with 20 to 24 carbon atoms (ΣC20-24) in fish receiving carnitine supplemented diets indicating an increased utilization of the above fatty acids. Free carnitine and acid-soluble carnitine in muscle increased two fold after carnitine administration.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{zouros_negative_1996,

title = {The negative correlation between somatic aneuploidy and growth in the oyster Crassostrea gigas and implications for the effects of induced polyploidization},

author = {E Zouros and C Thiriot-Quievreux and G Kotoulas},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029827355&partnerID=40&md5=521daa399a6f0c6e7a91a097fde9d06d},

issn = {00166723},

year  = {1996},

date = {1996-01-01},

journal = {Genetical Research},

volume = {68},

number = {2},

pages = {109--116},

abstract = {This study extends previous observations that chromosome loss in somatic cells of juveniles of the pacific oyster Crassostrea gigas is associated with reduced growth rate. All four studies designed to examine this association (two using random population samples and two using full sibs) produced the same result. This consistent effect appears to be unrelated with the commonly, but not consistently, observed correlation between degree of allozyme heterozygosity and growth. We propose that the inverse relationship between aneuploidy and growth is due to the unmasking of deleterious recessive genes caused by 'progressive haploidization' of somatic cells. Because unmasking of deleterious recessive genes by random chromosome loss is unlikely in polyploid cells, this hypothesis may also provide an explanation for the observation that artificially produced polyploid shellfish usually grow at faster rates than normal diploid ones.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{dando_earthquakes_1995,

title = {Earthquakes increase hydrothermal venting and nutrient inputs into the Aegean},

author = {P R Dando and J A Hughes and Y Leahy and L J Taylor and S Zivanovic},

url = {https://linkinghub.elsevier.com/retrieve/pii/0278434394E0031G},

doi = {10.1016/0278-4343(94)E0031-G},

issn = {02784343},

year  = {1995},

date = {1995-05-01},

urldate = {2020-08-17},

journal = {Continental Shelf Research},

volume = {15},

number = {6},

pages = {655--662},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nengas_investigation_1995,

title = {Investigation to determine digestibility coefficients of various raw materials in diets for gilthead sea bream, Sparus auratus L.},

author = {I Nengas and M N Alexis and S J Davies and G Petichakis},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0029132532&partnerID=40&md5=9689c28ef976e73848ed195bdd701739},

doi = {10.1111/j.1365-2109.1995.tb00901.x},

issn = {1355-557X, 1365-2109},

year  = {1995},

date = {1995-03-01},

journal = {Aquaculture Research},

volume = {26},

number = {3},

pages = {185--194},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{arvanitidis_amphitritides_1995,

title = {Amphitritides kuehlmanni sp. nov. (Polychaeta, terebellidae, amphitritinae) From the aegean sea, with comments on the genus amphitritides augener},

author = {C Arvanitidis and A Koukouras},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0008052656&doi=10.1080%2f00785326.1995.10430587&partnerID=40&md5=1d9f4ac3229430ce5afd424dee8fc704},

doi = {10.1080/00785326.1995.10430587},

issn = {00785326},

year  = {1995},

date = {1995-01-01},

journal = {Ophelia},

volume = {40},

number = {3},

pages = {219--227},

abstract = {A new species of Terebellidae Polychaeta, Amphitritides kuehlmanni, is described from shallow waters in the North Aegean. The new species is compared to the other known species of the genus. The status of the genus Amphitritides Augener is discussed. © 1995, Taylor & Francis Group. All rights reserved.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{kotoulas_genetic_1995,

title = {Genetic structure of the common sole Solea vulgaris at different geographic scales},

author = {G Kotoulas and F Bonhomme and P Borsa},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029414659&doi=10.1007%2fBF00350869&partnerID=40&md5=efae5e82aeadebd78b94d317e2406129},

doi = {10.1007/BF00350869},

issn = {00253162},

year  = {1995},

date = {1995-01-01},

journal = {Marine Biology},

volume = {122},

number = {3},

pages = {361--375},

abstract = {The genetic structure of the flatfish Solea vulgaris was investigated on several spatial scales and at the temporal level through analysis of electrophoretic variation at 8 to 12 polymorphic enzyme loci. No differentiation was apparent at the temporal scale. Some differentiation was detected at and above the regional scale. Isolation by distance was evidenced by the significant correlation between genetic and geographic distances, and by the consistency of the results of multiple-locus correspondence analysis with geographic sampling patterns. The analysis suggested that the geographic unit of population structure (i.e. a geographical area corresponding to a panmictic or nearly panmictic population) lies within a radius of the order of 100 km. The isolation-by-distance pattern in S. vulgaris contrasted with the known genetic structures of other flatfish species of the northeastern Atlantic and Mediterranean in a way that may be related to the range of their respective temperature tolerances for eggs and larvae. © 1995 Springer-Verlag.},

note = {Publisher: Springer-Verlag},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{mylonas_induced_1995,

title = {Induced Spawning of Wild American Shad Alosa sapidissima Using Sustained Administration of Gonadotropin‐Releasing Hormone Analog (GnRHa)},

author = {C C Mylonas and Y Zohar and B M Richardson and S P Minkkinen},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-21844523161&doi=10.1111%2fj.1749-7345.1995.tb00252.x&partnerID=40&md5=279c3a312c53a6e1e1b8376db7bb6988},

doi = {10.1111/j.1749-7345.1995.tb00252.x},

issn = {08938849},

year  = {1995},

date = {1995-01-01},

journal = {Journal of the World Aquaculture Society},

volume = {26},

number = {3},

pages = {240--251},

abstract = {American shad Alosa supidissima broodstock were collected from the Susquehanna River during their spawning migration. Mean volume of expressible milt (± standard deviation) was 2.5 (±1.7) mL/kg body weight; mean spermatozoid count was 66.2 ± 109 (±163 ± 109) spermntozoa/mL; and duration of 50% motility was 36.5 (±10.3) see. Ovarian biopsies indicated the presence of oocytes of various sizes (200–2,000 μm in diameter) and stages of development. Fish were implanted with a delivery system loaded with gonadotropin‐releasing hormone analog (GnRHa) and started spawning 2 d after treatment. Fertile eggs were collected daily for the next 9 d, for a total of 50,100 eggs/kg body weight with a mean fertilization success of 62%. Upon cessation of spawning, the ovaries of all females still contained large numbers of oocytes at various stages of development, as at the beginning of the experiment, but with a greater number of atretic oacytes. Our observations show that American shad have an asynchronous ovarian development, and treatment with a GnRHa delivery system is effective in inducing several successive spawns of fertile eggs. Copyright © 1995, Wiley Blackwell. All rights reserved},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{kotoulas_marked_1995,

title = {Marked mitochondrial DNA differences between Mediterranean and Atlantic populations of the swordfish, Xiphias gladius},

author = {Georgios Kotoulas and Antonios Magoulas and N Tsimenides and E Zouros},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028874118&doi=10.1111%2fj.1365-294X.1995.tb00241.x&partnerID=40&md5=529a83d003e7708c8e88bcf66c58b29e},

doi = {10.1111/j.1365-294X.1995.tb00241.x},

issn = {09621083},

year  = {1995},

date = {1995-01-01},

journal = {Molecular Ecology},

volume = {4},

number = {4},

pages = {473--482},

abstract = {Restriction analysis of mitochondrial DNA (mtDNA) from 204 individuals of swordfish (Xiphias gladius) revealed no differentiation among samples from three sites in the Mediterranean Sea (Greece, Italy, Spain), but a high degree of differentiation between Mediterranean samples and a sample from the Gulf of Guinea. A fifth sample from the Atlantic side of the Straits of Gibraltar (Tarifa) consisted mostly of mitotypes that are common in the Mediterranean, but contained several of mtDNA types of the Guinea sample not found in the Mediterranean. We conclude that, in spite of free migration of swordfish across the Straits of Gibraltar, little genetic exchange occurs between the populations inhabiting the Mediterranean Sea and the tropical Atlantic ocean. This is the first evidence of genetic differentiation among geographic populations of this highly mobile species that supports a world‐wide fishery. Copyright © 1995, Wiley Blackwell. All rights reserved},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{magoulas_mitochondrial_1995,

title = {Mitochondrial DNA variation in an experimental stock of gilthead sea bream (Sparus aurata)},

author = {A Magoulas and K Sophronides and T Patarnello and E Hatzilaris and E Zouros},

year  = {1995},

date = {1995-01-01},

journal = {Molecular Marine Biology and Biotechnology},

volume = {4},

pages = {110--116},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{koumoundouros_morphometric_1995,

title = {Morphometric relationships as criteria for the evaluation of larval quality of gilthead sea bream},

author = {G Koumoundouros and Z Kiriakos and P Divanach and M Kentouri},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0001396165&partnerID=40&md5=37c948592073f3aa8dc5432b1793f12d},

year  = {1995},

date = {1995-01-01},

journal = {Aquaculture International},

volume = {3},

pages = {143 -- 149},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{mylonas_preparation_1995,

title = {Preparation and evaluation of polyanhydride microspheres containing gonadotropin-releasing hormone (GnRH), for inducing ovulation and spermiation in fish},

author = {C C Mylonas and Y Tabata and R Langer and Y Zohar},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029072968&doi=10.1016%2f0168-3659%2895%2900009-W&partnerID=40&md5=7ed29a4581d9db234fa69df97c5fbe8b},

doi = {10.1016/0168-3659(95)00009-W},

issn = {01683659},

year  = {1995},

date = {1995-01-01},

journal = {Journal of Controlled Release},

volume = {35},

number = {1},

pages = {23--34},

abstract = {We developed a microspheric delivery system for a synthetic analog of gonadotropin-releasing hormone (GnRHa) for inducing ovulation and enhancing spermiation in broodfish which, relative to mammals, are maintained at low temperatures (10-22°C). The microspheres were based on a biodegradable copolymer of fatty acid dimer and sebacic acid (p[FAD-SA]) of a 25:75 molar ratio, and were prepared by a solvent evaporation method, using double emulsion. The GnRH analog [d-Ala6, Pro9NEt]-GnRH was entrapped into the microspheres at 3% loading with 86% (± 5.4) efficiency. The prepared microspheres had a spherical shape, with diameters from 10 to 240 μm. In vitro, GnRHa was released for at least 90 days at 15°C, following an initial release of 8.8% of the total amount. The release during the first 30 days followed first-order kinetics described by the equation GnRHa (μg) = 1.7[day]0.33 (R2=0.99), and zero-order kinetics thereafter, described by the equation GnRHa (μg) = 0.027[day] + 4.5 (R2 = 0.98). Plasma GnRHa levels of microsphere-injected striped bass (Morone saxatilis) remained elevated for at least 8 weeks at 16°C, although the levels dropped dramatically after day 28. Microsphere injection of post-vitellogenic striped bass females and Atlantic salmon (Salmo salar) induced 100% ovulation by day 11 and 15 after treatment, respectively. Similar treatment induced a 4-10-fold increase in sperm production of striped bass males for at least 14 days after treatment and resulted in a 2.5-fold increase of expressible sperm volume of male Atlantic salmon 10 days after treatment. © 1995.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}