Broodstock management


Constantinos Mylonas

Mylonas, C.C.

Irene Sigelakis

Sigelaki, E

Maria Papadaki

Papadaki, M.

Ioannis Fakriadis

Fakriadis, I.

Stefano Lancerotto

Lancerotto, S

The facilities include tanks of various volumes (3 x 35 m3, 7 x 15 m3, 2 x 10 m3, 8 x 5 m3and 14 x 2 m3) for the maintenance of breeders of different species.  All but the smallest tanks can operate either in flow through or recirculation mode, and are equipped with temperature and photoperiod control using LED lights.  The species maintained include European sea bass (Dicentrarchus labrax), gilthead sea bream (Sparus aurata), sharpsnout sea bream (Diplodus puntazzo), shi drum (Umbrina cirrosa), meagre (Argyrosomus regius), and greater amberjack (Seriola dumerili).  Eggs are maintained in temperature controlled egg incubators, and larvae can be maintained until the end of yolk absorption.

A wet lab is used for the sampling (blood, gonadal biopsies and tissue extraction) and the initial evaluation of gamete and egg quality obtained from the various experimental and production broodstocks.  Light compound and stereo microscopes are used to evaluate the quality of oocytes obtained via ovarian biopsies, in order to monitor the stage of oogenesis and select females for hormonal spawning induction.  A computer assisted sperm analysis (CASA) system is available for the evaluation of sperm quality, looking at sperm motility characteristics.

Supporting the facility is an analytical laboratory equipped with a variety of equipment for the study of reproductive biology and endocrinology.  Hormonal analyses are done using ELISAs, and include the sex steroid hormones, gonadotropins and gonadotropin releasing hormone agonists (GnRHa). Histological evaluations are done using methacrylate resin and various stains.  Controlled temperature incubators are used for the evaluation of egg and larval quality using the micro-titer plate method. A spectrophotometer is used to monitor water quality in the broodstock tanks.  The lab is also equipped for the production of polymer-based, controlled-release devices for GnRHa, used for the induction of spawning and enhancement of sperm production (EVAc implants and PLGA microspheres).

Research directions (put the title-icon to link to specific page):

Fish reproduction and physiology

Services (put the title-icon to link to specific page):

Broodstock management consulting

Induced spawning implants

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