2023 |
Vardali, Sofia; Papadouli, Christina; Rigos, George; Nengas, Ioannis; Panagiotaki, Panagiota; Golomazou, Eleni Recent Advances in Mycotoxin Determination in Fish Feed Ingredients Journal Article Molecules, 28 (6), pp. 2519, 2023, ISSN: 1420-3049. @article{vardali_recent_2023, title = {Recent Advances in Mycotoxin Determination in Fish Feed Ingredients}, author = {Sofia Vardali and Christina Papadouli and George Rigos and Ioannis Nengas and Panagiota Panagiotaki and Eleni Golomazou}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/03/2023-Vardali-Mol-15-1.pdf https://www.mdpi.com/1420-3049/28/6/2519}, doi = {10.3390/molecules28062519}, issn = {1420-3049}, year = {2023}, date = {2023-03-13}, urldate = {2023-03-13}, journal = {Molecules}, volume = {28}, number = {6}, pages = {2519}, abstract = {Low-cost plant-based sources used in aquaculture diets are prone to the occurrence of animal feed contaminants, which may in certain conditions affect the quality and safety of aquafeeds. Mycotoxins, a toxic group of small organic molecules produced by fungi, comprise a frequently occurring plant-based feed contaminant in aquafeeds. Mycotoxin contamination can potentially cause significant mortality, reduced productivity, and higher disease susceptibility; thus, its timely detection is crucial to the aquaculture industry. The present review summarizes the methodological advances, developed mainly during the past decade, related to mycotoxin detection in aquafeed ingredients, namely analytical, chromatographic, and immunological methodologies, as well as the use of biosensors and spectroscopic methods which are becoming more prevalent. Rapid and accurate mycotoxin detection is and will continue to be crucial to the food industry, animal production, and the environment, resulting in further improvements and developments in mycotoxin detection techniques.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Low-cost plant-based sources used in aquaculture diets are prone to the occurrence of animal feed contaminants, which may in certain conditions affect the quality and safety of aquafeeds. Mycotoxins, a toxic group of small organic molecules produced by fungi, comprise a frequently occurring plant-based feed contaminant in aquafeeds. Mycotoxin contamination can potentially cause significant mortality, reduced productivity, and higher disease susceptibility; thus, its timely detection is crucial to the aquaculture industry. The present review summarizes the methodological advances, developed mainly during the past decade, related to mycotoxin detection in aquafeed ingredients, namely analytical, chromatographic, and immunological methodologies, as well as the use of biosensors and spectroscopic methods which are becoming more prevalent. Rapid and accurate mycotoxin detection is and will continue to be crucial to the food industry, animal production, and the environment, resulting in further improvements and developments in mycotoxin detection techniques. |
Baltoumas, Fotis A; Karatzas, Evangelos; Paez-Espino, David; Venetsianou, Nefeli K; Aplakidou, Eleni; Oulas, Anastasis; Finn, Robert D; Ovchinnikov, Sergey; Pafilis, Evangelos; Kyrpides, Nikos C; Pavlopoulos, Georgios A Exploring microbial functional biodiversity at the protein family level—From metagenomic sequence reads to annotated protein clusters Journal Article Frontiers in Bioinformatics, 3 , pp. 1157956, 2023, ISSN: 2673-7647. @article{baltoumas_exploring_2023, title = {Exploring microbial functional biodiversity at the protein family level—From metagenomic sequence reads to annotated protein clusters}, author = {Fotis A Baltoumas and Evangelos Karatzas and David Paez-Espino and Nefeli K Venetsianou and Eleni Aplakidou and Anastasis Oulas and Robert D Finn and Sergey Ovchinnikov and Evangelos Pafilis and Nikos C Kyrpides and Georgios A Pavlopoulos}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/03/2023-Baltoumas-FroΒionform-14.pdf https://www.frontiersin.org/articles/10.3389/fbinf.2023.1157956/full}, doi = {10.3389/fbinf.2023.1157956}, issn = {2673-7647}, year = {2023}, date = {2023-03-08}, urldate = {2023-03-08}, journal = {Frontiers in Bioinformatics}, volume = {3}, pages = {1157956}, abstract = {Metagenomics has enabled accessing the genetic repertoire of natural microbial communities. Metagenome shotgun sequencing has become the method of choice for studying and classifying microorganisms from various environments. To this end, several methods have been developed to process and analyze the sequence data from raw reads to end-products such as predicted protein sequences or families. In this article, we provide a thorough review to simplify such processes and discuss the alternative methodologies that can be followed in order to explore biodiversity at the protein family level. We provide details for analysis tools and we comment on their scalability as well as their advantages and disadvantages. Finally, we report the available data repositories and recommend various approaches for protein family annotation related to phylogenetic distribution, structure prediction and metadata enrichment.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Metagenomics has enabled accessing the genetic repertoire of natural microbial communities. Metagenome shotgun sequencing has become the method of choice for studying and classifying microorganisms from various environments. To this end, several methods have been developed to process and analyze the sequence data from raw reads to end-products such as predicted protein sequences or families. In this article, we provide a thorough review to simplify such processes and discuss the alternative methodologies that can be followed in order to explore biodiversity at the protein family level. We provide details for analysis tools and we comment on their scalability as well as their advantages and disadvantages. Finally, we report the available data repositories and recommend various approaches for protein family annotation related to phylogenetic distribution, structure prediction and metadata enrichment. |
Tsertou, Maria Ioanna; Triga, Adriana; Droubogiannis, Stavros; Kokkari, Constantina; Anasi, Grammatiki; Katharios, Pantelis Frontiers in Microbiology, 14 , pp. 1078669, 2023, ISSN: 1664-302X. @article{tsertou_isolation_2023, title = {Isolation and characterization of a novel Tenacibaculum species and a corresponding bacteriophage from a Mediterranean fish hatchery: Description of Tenacibaculum larymnensis sp. nov. and Tenacibaculum phage Larrie}, author = {Maria Ioanna Tsertou and Adriana Triga and Stavros Droubogiannis and Constantina Kokkari and Grammatiki Anasi and Pantelis Katharios}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/03/2023-Tsertou-FroMicro-13.pdf https://www.frontiersin.org/articles/10.3389/fmicb.2023.1078669/full}, doi = {10.3389/fmicb.2023.1078669}, issn = {1664-302X}, year = {2023}, date = {2023-03-07}, urldate = {2023-03-07}, journal = {Frontiers in Microbiology}, volume = {14}, pages = {1078669}, abstract = {Tenacibaculum larymnensis sp. nov., a novel species of the Tenacibaculum genus was isolated from a commercial fish hatchery in Greece. The novel species is phylogenetically close to T. discolor and was biochemically and genetically characterized. The genome of T . larymnensis has 3.66 Mbps length, 31.83% GC content and the genomic analysis demonstrated that it harbors a wide enzymatic repertoire suggestive of increased degrading capacity but also several virulence factors including hemolysins, secretion systems, transporters, siderophores, pili and extracellular proteins. Using the novel strain, a virulent bacteriophage designated as Tenacibaculum phage Larrie was isolated and characterized. Larrie is a novel Siphovirus with relatively large genome, 77.5 kbps with 111 ORFs, a GC content of 33.7% and an exclusively lytic lifestyle. The new phage-host system can serve as an efficient model to study microbial interactions in the aquatic environment which contribute to the nutrient cycling.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Tenacibaculum larymnensis sp. nov., a novel species of the Tenacibaculum genus was isolated from a commercial fish hatchery in Greece. The novel species is phylogenetically close to T. discolor and was biochemically and genetically characterized. The genome of T . larymnensis has 3.66 Mbps length, 31.83% GC content and the genomic analysis demonstrated that it harbors a wide enzymatic repertoire suggestive of increased degrading capacity but also several virulence factors including hemolysins, secretion systems, transporters, siderophores, pili and extracellular proteins. Using the novel strain, a virulent bacteriophage designated as Tenacibaculum phage Larrie was isolated and characterized. Larrie is a novel Siphovirus with relatively large genome, 77.5 kbps with 111 ORFs, a GC content of 33.7% and an exclusively lytic lifestyle. The new phage-host system can serve as an efficient model to study microbial interactions in the aquatic environment which contribute to the nutrient cycling. |
Garza, Daniel Rios; Gonze, Didier; Zafeiropoulos, Haris; Liu, Bin; Faust, Karoline Metabolic models of human gut microbiota: Advances and challenges Journal Article Cell Systems, 14 (2), pp. 109–121, 2023, ISSN: 24054712. @article{rios_garza_metabolic_2023, title = {Metabolic models of human gut microbiota: Advances and challenges}, author = {Daniel Rios Garza and Didier Gonze and Haris Zafeiropoulos and Bin Liu and Karoline Faust}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/02/2023-Rios-Garza-Cell-Systems-reprint12.pdf https://linkinghub.elsevier.com/retrieve/pii/S2405471222004379}, doi = {10.1016/j.cels.2022.11.002}, issn = {24054712}, year = {2023}, date = {2023-02-28}, urldate = {2023-02-28}, journal = {Cell Systems}, volume = {14}, number = {2}, pages = {109--121}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Kalatzis, Panos G; Mauritzen, Jesper Juel; Winther-Have, Caroline Sophie; Michniewski, Slawomir; Millard, Andrew; Tsertou, Maria Ioanna; Katharios, Pantelis; Middelboe, Mathias International Journal of Molecular Sciences, 24 (4), pp. 3937, 2023, ISSN: 1422-0067. @article{kalatzis_staying_2023, title = {Staying below the Radar: Unraveling a New Family of Ubiquitous “Cryptic” Non-Tailed Temperate Vibriophages and Implications for Their Bacterial Hosts}, author = {Panos G Kalatzis and Jesper Juel Mauritzen and Caroline Sophie Winther-Have and Slawomir Michniewski and Andrew Millard and Maria Ioanna Tsertou and Pantelis Katharios and Mathias Middelboe}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/02/2023-Kalatzis-IJMolSci-11.pdf https://www.mdpi.com/1422-0067/24/4/3937}, doi = {10.3390/ijms24043937}, issn = {1422-0067}, year = {2023}, date = {2023-02-17}, urldate = {2023-02-20}, journal = {International Journal of Molecular Sciences}, volume = {24}, number = {4}, pages = {3937}, abstract = {Bacteriophages are the most abundant biological entities in the oceans and play key roles in bacterial activity, diversity and evolution. While extensive research has been conducted on the role of tailed viruses (Class: Caudoviricetes), very little is known about the distribution and functions of the non-tailed viruses (Class: Tectiliviricetes). The recent discovery of the lytic Autolykiviridae family demonstrated the potential importance of this structural lineage, emphasizing the need for further exploration of the role of this group of marine viruses. Here, we report the novel family of temperate phages under the class of Tectiliviricetes, which we propose to name “Asemoviridae” with phage NO16 as a main representative. These phages are widely distributed across geographical regions and isolation sources and found inside the genomes of at least 30 species of Vibrio, in addition to the original V. anguillarum isolation host. Genomic analysis identified dif-like sites, suggesting that NO16 prophages recombine with the bacterial genome based on the XerCD site-specific recombination mechanism. The interactions between the NO16 phage and its V. anguillarum host were linked to cell density and phage–host ratio. High cell density and low phage predation levels were shown to favor the temperate over the lytic lifestyle for NO16 viruses, and their spontaneous induction rate was highly variable between different V. anguillarum lysogenic strains. NO16 prophages coexist with the V. anguillarum host in a mutualistic interaction by rendering fitness properties to the host, such as increased virulence and biofilm formation through lysogenic conversion, likely contributing to their global distribution.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Bacteriophages are the most abundant biological entities in the oceans and play key roles in bacterial activity, diversity and evolution. While extensive research has been conducted on the role of tailed viruses (Class: Caudoviricetes), very little is known about the distribution and functions of the non-tailed viruses (Class: Tectiliviricetes). The recent discovery of the lytic Autolykiviridae family demonstrated the potential importance of this structural lineage, emphasizing the need for further exploration of the role of this group of marine viruses. Here, we report the novel family of temperate phages under the class of Tectiliviricetes, which we propose to name “Asemoviridae” with phage NO16 as a main representative. These phages are widely distributed across geographical regions and isolation sources and found inside the genomes of at least 30 species of Vibrio, in addition to the original V. anguillarum isolation host. Genomic analysis identified dif-like sites, suggesting that NO16 prophages recombine with the bacterial genome based on the XerCD site-specific recombination mechanism. The interactions between the NO16 phage and its V. anguillarum host were linked to cell density and phage–host ratio. High cell density and low phage predation levels were shown to favor the temperate over the lytic lifestyle for NO16 viruses, and their spontaneous induction rate was highly variable between different V. anguillarum lysogenic strains. NO16 prophages coexist with the V. anguillarum host in a mutualistic interaction by rendering fitness properties to the host, such as increased virulence and biofilm formation through lysogenic conversion, likely contributing to their global distribution. |
Nanou, Evangelia; Kotsiri, Mado; Kogiannou, Dimitra; Katsouli, Maria; Grigorakis, Kriton Foods, 12 (3), pp. 505, 2023, ISSN: 2304-8158. @article{nanou_consumer_2023, title = {Consumer Perception of Freshness and Volatile Composition of Fresh Gilthead Seabream and Seabass in Active Packaging with and without CO2-Emitting Pads}, author = {Evangelia Nanou and Mado Kotsiri and Dimitra Kogiannou and Maria Katsouli and Kriton Grigorakis}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/02/2023-Nanou-foods-10.pdf https://www.mdpi.com/2304-8158/12/3/505}, doi = {10.3390/foods12030505}, issn = {2304-8158}, year = {2023}, date = {2023-02-14}, urldate = {2023-02-15}, journal = {Foods}, volume = {12}, number = {3}, pages = {505}, abstract = {Active packaging with CO2-emitters (pads) has recently been used for shelf-life extension of fresh fish. The aim of this study was to identify consumer attitudes towards fresh fish packaging, to examine whether Greek consumers prefer active packaging with pad over active packaging without pad, to investigate any perceived differences in the sensory freshness of the fish, and to relate consumer perception to volatile composition of fish fillets. In total, 274 consumers participated in the study which included freshness sensory evaluation of gilthead seabream (Sparus aurata) and seabass (Dicentrarchuslabrax), whole-gutted and filleted, raw and cooked, at high quality and at the end of high-quality shelf-life. Samples were packed under modified atmosphere either with a pad or without. Results showed that consumers preferred packages with pads, especially at the end of high quality shelf-life. They perceived raw samples packed with a pad to be fresher and closer to the ideal product, and also had a higher purchase intention towards them. Cooked samples were not perceived differently. Consumers’ perception was in accordance with the GC-MS findings in the volatile compounds that function as freshness or spoilage indicators. Most participants were positive towards fresh fish packaging although they usually buy unpacked fresh fish. Our results suggest that active packaging with CO2 emitters contribute to freshness preservation and that it has a positive potential in the Greek market.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Active packaging with CO2-emitters (pads) has recently been used for shelf-life extension of fresh fish. The aim of this study was to identify consumer attitudes towards fresh fish packaging, to examine whether Greek consumers prefer active packaging with pad over active packaging without pad, to investigate any perceived differences in the sensory freshness of the fish, and to relate consumer perception to volatile composition of fish fillets. In total, 274 consumers participated in the study which included freshness sensory evaluation of gilthead seabream (Sparus aurata) and seabass (Dicentrarchuslabrax), whole-gutted and filleted, raw and cooked, at high quality and at the end of high-quality shelf-life. Samples were packed under modified atmosphere either with a pad or without. Results showed that consumers preferred packages with pads, especially at the end of high quality shelf-life. They perceived raw samples packed with a pad to be fresher and closer to the ideal product, and also had a higher purchase intention towards them. Cooked samples were not perceived differently. Consumers’ perception was in accordance with the GC-MS findings in the volatile compounds that function as freshness or spoilage indicators. Most participants were positive towards fresh fish packaging although they usually buy unpacked fresh fish. Our results suggest that active packaging with CO2 emitters contribute to freshness preservation and that it has a positive potential in the Greek market. |
Antoniou, Aglaia; Manousaki, Tereza; Ramírez, Francisco; Cariani, Alessia; Cannas, Rita; Kasapidis, Panagiotis; Magoulas, Antonios; Albo‐Puigserver, Marta; Lloret‐Lloret, Elena; Bellido, Jose Maria; Pennino, Maria Grazia; Follesa, Maria Cristina; Esteban, Antonio; Saraux, Claire; Sbrana, Mario; Spedicato, Maria Teresa; Coll, Marta; Tsigenopoulos, Costas S Sardines at a junction: Seascape genomics reveals ecological and oceanographic drivers of variation in the textlessspan style= Journal Article Molecular Ecology, pp. mec.16840, 2023, ISSN: 0962-1083, 1365-294X. @article{antoniou_sardines_2023, title = {Sardines at a junction: Seascape genomics reveals ecological and oceanographic drivers of variation in the textlessspan style=}, author = {Aglaia Antoniou and Tereza Manousaki and Francisco Ramírez and Alessia Cariani and Rita Cannas and Panagiotis Kasapidis and Antonios Magoulas and Marta Albo‐Puigserver and Elena Lloret‐Lloret and Jose Maria Bellido and Maria Grazia Pennino and Maria Cristina Follesa and Antonio Esteban and Claire Saraux and Mario Sbrana and Maria Teresa Spedicato and Marta Coll and Costas S Tsigenopoulos}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/02/2023-Antoniou-Mol-Eco-9-1.pdf https://onlinelibrary.wiley.com/doi/10.1111/mec.16840}, doi = {10.1111/mec.16840}, issn = {0962-1083, 1365-294X}, year = {2023}, date = {2023-02-06}, urldate = {2023-02-06}, journal = {Molecular Ecology}, pages = {mec.16840}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Dimogianopoulos, D; Grigorakis, K Effective algorithmic operational framework for fish texture evaluation in industry: Achieving maturity Journal Article Aquaculture and Fisheries, 8 (4), pp. 422–430, 2023, ISSN: 2468550X. @article{dimogianopoulos_effective_2023, title = {Effective algorithmic operational framework for fish texture evaluation in industry: Achieving maturity}, author = {D Dimogianopoulos and K Grigorakis}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/01/2023-Dimogiannopoulos-AandF-7.pdf https://linkinghub.elsevier.com/retrieve/pii/S2468550X20301386}, doi = {10.1016/j.aaf.2020.10.001}, issn = {2468550X}, year = {2023}, date = {2023-01-31}, urldate = {2023-01-31}, journal = {Aquaculture and Fisheries}, volume = {8}, number = {4}, pages = {422--430}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Pavloudi, Christina; Tsertou, Maria Ioanna; Antonopoulou, Efthimia; Katharios, Pantelis Investigation of systemic granulomatosis in cultured meagre, Argyrosomus regius, using clinical metagenomics Journal Article Aquaculture, 567 , pp. 739249, 2023, ISSN: 00448486. @article{pavloudi_investigation_2023, title = {Investigation of systemic granulomatosis in cultured meagre, Argyrosomus regius, using clinical metagenomics}, author = {Christina Pavloudi and Maria Ioanna Tsertou and Efthimia Antonopoulou and Pantelis Katharios}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/01/2023-Pavloudi-AQUA-pre-print-8.pdf https://linkinghub.elsevier.com/retrieve/pii/S0044848623000224}, doi = {10.1016/j.aquaculture.2023.739249}, issn = {00448486}, year = {2023}, date = {2023-01-25}, urldate = {2023-01-25}, journal = {Aquaculture}, volume = {567}, pages = {739249}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Rees, Andrew P; Faraggiana, Eleonora; Tait, Karen; Celussi, Mauro; Dafnomilli, Eleni; Manna, Vincenzo; Manning, Andrew; Pitta, Paraskevi; Tsiola, Anastasia; Živanović, Snežana Frontiers in Marine Science, 9 , pp. 907269, 2023, ISSN: 2296-7745. @article{rees_presence_2022, title = {The presence of silver nanoparticles reduces demand for dissolved phosphorus to the benefit of biological nitrogen fixation in the coastal eastern Mediterranean Sea}, author = {Andrew P Rees and Eleonora Faraggiana and Karen Tait and Mauro Celussi and Eleni Dafnomilli and Vincenzo Manna and Andrew Manning and Paraskevi Pitta and Anastasia Tsiola and Snežana Živanović}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/01/2023-Rees-fmars-6.pdf https://www.frontiersin.org/articles/10.3389/fmars.2022.907269/full}, doi = {10.3389/fmars.2022.907269}, issn = {2296-7745}, year = {2023}, date = {2023-01-23}, urldate = {2023-01-23}, journal = {Frontiers in Marine Science}, volume = {9}, pages = {907269}, abstract = {The release of silver into the marine environment is of growing concern as its impact on marine life is not fully understood. Despite previous experiments that have shown toxic effects of silver as nanoparticles (AgNPs) and as free ions (Ag + ) on microbial organisms, the impact on important biogeochemical processes, such as marine nitrogen fixation, remains relatively unexplored. The present study investigated the impact of AgNPs and Ag + on nitrogen fixation activity in oligotrophic coastal ecosystems. Nine mesocosm enclosures were set-up in Crete, Greece, for twelve days during May 2019. Three mesocosms were left unamended at ambient light and temperature; three were manipulated with 50 ng AgNPs L -1 d -1 and three were amended with 50 ng Ag + L -1 d -1 . Over the duration of the experiment, mean nitrogen fixation rates proved higher in treated waters; 0.28 ± 0.24 nmol N L -1 d -1 and 0.21 ± 0.19 nmol N L -1 d -1 in AgNP and Ag + which were 2.2 (p< 0.001) and 1.6 (not significant) times higher than control rates of 0.13 ± 0.07 nmol N L -1 d -1 respectively. Changes in nitrogen fixation rates were paralleled by significantly higher concentrations of phosphate and silicate in treated versus control mesocosms, suggesting an inhibition of the uptake of these nutrients by non-diazotrophic groups. Sequencing of 16S and 18S rRNA genes showed shifts in community composition over time but only very minor changes could be linked to the Ag treatments. Decreases to the relative abundance of three diatom species were observed in the Ag treatments but these were considered insufficient to support the relative differences in nutrient concentration. It is therefore speculated that there was physiological disruption of nutrient uptake mechanisms of the non-diazotrophic community. A number of potential diazotrophs were detected and the relative sequence abundance of a number of known nitrogen fixing taxa, including Burkholderiaceae , Oceanospirillales and Pseudomonadales correlated with measured nitrogen fixation rates. Phosphorus limitation of diazotrophic activity was therefore lowered relative to other microbial groups in silver amended treatments and significant increases in particulate nitrogen for both Ag treatments indicate an enhancement in cellular N for the nitrogen fixing communities.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The release of silver into the marine environment is of growing concern as its impact on marine life is not fully understood. Despite previous experiments that have shown toxic effects of silver as nanoparticles (AgNPs) and as free ions (Ag + ) on microbial organisms, the impact on important biogeochemical processes, such as marine nitrogen fixation, remains relatively unexplored. The present study investigated the impact of AgNPs and Ag + on nitrogen fixation activity in oligotrophic coastal ecosystems. Nine mesocosm enclosures were set-up in Crete, Greece, for twelve days during May 2019. Three mesocosms were left unamended at ambient light and temperature; three were manipulated with 50 ng AgNPs L -1 d -1 and three were amended with 50 ng Ag + L -1 d -1 . Over the duration of the experiment, mean nitrogen fixation rates proved higher in treated waters; 0.28 ± 0.24 nmol N L -1 d -1 and 0.21 ± 0.19 nmol N L -1 d -1 in AgNP and Ag + which were 2.2 (p< 0.001) and 1.6 (not significant) times higher than control rates of 0.13 ± 0.07 nmol N L -1 d -1 respectively. Changes in nitrogen fixation rates were paralleled by significantly higher concentrations of phosphate and silicate in treated versus control mesocosms, suggesting an inhibition of the uptake of these nutrients by non-diazotrophic groups. Sequencing of 16S and 18S rRNA genes showed shifts in community composition over time but only very minor changes could be linked to the Ag treatments. Decreases to the relative abundance of three diatom species were observed in the Ag treatments but these were considered insufficient to support the relative differences in nutrient concentration. It is therefore speculated that there was physiological disruption of nutrient uptake mechanisms of the non-diazotrophic community. A number of potential diazotrophs were detected and the relative sequence abundance of a number of known nitrogen fixing taxa, including Burkholderiaceae , Oceanospirillales and Pseudomonadales correlated with measured nitrogen fixation rates. Phosphorus limitation of diazotrophic activity was therefore lowered relative to other microbial groups in silver amended treatments and significant increases in particulate nitrogen for both Ag treatments indicate an enhancement in cellular N for the nitrogen fixing communities. |
Bauer, Sven Le Moine; Lu, Guang-Sin; Goulaouic, Steven; Puzenat, Valentine; Schouw, Anders; Barreyre, Thibaut; Pawlowsky-Glahn, Vera; Egozcue, Juan José; Martelat, Jean-Emmanuel; Escartin, Javier; Amend, Jan P; Nomikou, Paraskevi; Vlasopoulos, Othonas; Polymenakou, Paraskevi; Jørgensen, Steffen Leth Structure and metabolic potential of the prokaryotic communities from the hydrothermal system of Paleochori Bay, Milos, Greece Journal Article Frontiers in Microbiology, 13 , pp. 1060168, 2023, ISSN: 1664-302X. @article{le_moine_bauer_structure_2023, title = {Structure and metabolic potential of the prokaryotic communities from the hydrothermal system of Paleochori Bay, Milos, Greece}, author = {Sven Le Moine Bauer and Guang-Sin Lu and Steven Goulaouic and Valentine Puzenat and Anders Schouw and Thibaut Barreyre and Vera Pawlowsky-Glahn and Juan José Egozcue and Jean-Emmanuel Martelat and Javier Escartin and Jan P Amend and Paraskevi Nomikou and Othonas Vlasopoulos and Paraskevi Polymenakou and Steffen Leth Jørgensen}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/01/2023-Le-Moine-Bauer-FinMicro-5.pdf https://www.frontiersin.org/articles/10.3389/fmicb.2022.1060168/full}, doi = {10.3389/fmicb.2022.1060168}, issn = {1664-302X}, year = {2023}, date = {2023-01-16}, urldate = {2023-01-16}, journal = {Frontiers in Microbiology}, volume = {13}, pages = {1060168}, abstract = {Introduction Shallow hydrothermal systems share many characteristics with their deep-sea counterparts, but their accessibility facilitates their study. One of the most studied shallow hydrothermal vent fields lies at Paleochori Bay off the coast of Milos in the Aegean Sea (Greece). It has been studied through extensive mapping and its physical and chemical processes have been characterized over the past decades. However, a thorough description of the microbial communities inhabiting the bay is still missing. Methods We present the first in-depth characterization of the prokaryotic communities of Paleochori Bay by sampling eight different seafloor types that are distributed along the entire gradient of hydrothermal influence. We used deep sequencing of the 16S rRNA marker gene and complemented the analysis with qPCR quantification of the 16S rRNA gene and several functional genes to gain insights into the metabolic potential of the communities. Results We found that the microbiome of the bay is strongly influenced by the hydrothermal venting, with a succession of various groups dominating the sediments from the coldest to the warmest zones. Prokaryotic diversity and abundance decrease with increasing temperature, and thermophilic archaea overtake the community. Discussion Relevant geochemical cycles of the Bay are discussed. This study expands our limited understanding of subsurface microbial communities in acidic shallow-sea hydrothermal systems and the contribution of their microbial activity to biogeochemical cycling.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Introduction Shallow hydrothermal systems share many characteristics with their deep-sea counterparts, but their accessibility facilitates their study. One of the most studied shallow hydrothermal vent fields lies at Paleochori Bay off the coast of Milos in the Aegean Sea (Greece). It has been studied through extensive mapping and its physical and chemical processes have been characterized over the past decades. However, a thorough description of the microbial communities inhabiting the bay is still missing. Methods We present the first in-depth characterization of the prokaryotic communities of Paleochori Bay by sampling eight different seafloor types that are distributed along the entire gradient of hydrothermal influence. We used deep sequencing of the 16S rRNA marker gene and complemented the analysis with qPCR quantification of the 16S rRNA gene and several functional genes to gain insights into the metabolic potential of the communities. Results We found that the microbiome of the bay is strongly influenced by the hydrothermal venting, with a succession of various groups dominating the sediments from the coldest to the warmest zones. Prokaryotic diversity and abundance decrease with increasing temperature, and thermophilic archaea overtake the community. Discussion Relevant geochemical cycles of the Bay are discussed. This study expands our limited understanding of subsurface microbial communities in acidic shallow-sea hydrothermal systems and the contribution of their microbial activity to biogeochemical cycling. |
Papadogiannis, Vasileios; Manousaki, Tereza; Nousias, Orestis; Tsakogiannis, Alexandros; Kristoffersen, Jon B; Mylonas, Constantinos C; Batargias, Costas; Chatziplis, Dimitrios; Tsigenopoulos, Costas S Chromosome genome assembly for the meagre, Argyrosomus regius, reveals species adaptations and sciaenid sex-related locus evolution Journal Article Frontiers in Genetics, 13 , pp. 1081760, 2023, ISSN: 1664-8021. @article{papadogiannis_chromosome_2023, title = {Chromosome genome assembly for the meagre, Argyrosomus regius, reveals species adaptations and sciaenid sex-related locus evolution}, author = {Vasileios Papadogiannis and Tereza Manousaki and Orestis Nousias and Alexandros Tsakogiannis and Jon B Kristoffersen and Constantinos C Mylonas and Costas Batargias and Dimitrios Chatziplis and Costas S Tsigenopoulos}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/01/2023-Papadogiannis-FinGenetics-4.pdf https://www.frontiersin.org/articles/10.3389/fgene.2022.1081760/full}, doi = {10.3389/fgene.2022.1081760}, issn = {1664-8021}, year = {2023}, date = {2023-01-10}, urldate = {2023-01-11}, journal = {Frontiers in Genetics}, volume = {13}, pages = {1081760}, abstract = {The meagre, Argyrosomus regius , has recently become a species of increasing economic interest for the Mediterranean aquaculture and there is ongoing work to boost production efficiency through selective breeding. Access to the complete genomic sequence will provide an essential resource for studying quantitative trait-associated loci and exploring the genetic diversity of different wild populations and aquaculture stocks in more detail. Here, we present the first complete genome for A. regius , produced through a combination of long and short read technologies and an efficient in-house developed pipeline for assembly and polishing. Scaffolding using previous linkage map data allowed us to reconstruct a chromosome level assembly with high completeness, complemented with gene annotation and repeat masking. The 696 Mb long assembly has an N50 = 27.87 Mb and an L50 = 12, with 92.85% of its length placed in 24 chromosomes. We use this new resource to study the evolution of the meagre genome and other Sciaenids, via a comparative analysis of 25 high-quality teleost genomes. Combining a rigorous investigation of gene duplications with base-wise conservation analysis, we identify candidate loci related to immune, fat metabolism and growth adaptations in the meagre. Following phylogenomic reconstruction, we show highly conserved synteny within Sciaenidae. In contrast, we report rapidly evolving syntenic rearrangements and gene copy changes in the sex-related dmrt1 neighbourhood in meagre and other members of the family. These novel genomic datasets and findings will add important new tools for aquaculture studies and greatly facilitate husbandry and breeding work in the species.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The meagre, Argyrosomus regius , has recently become a species of increasing economic interest for the Mediterranean aquaculture and there is ongoing work to boost production efficiency through selective breeding. Access to the complete genomic sequence will provide an essential resource for studying quantitative trait-associated loci and exploring the genetic diversity of different wild populations and aquaculture stocks in more detail. Here, we present the first complete genome for A. regius , produced through a combination of long and short read technologies and an efficient in-house developed pipeline for assembly and polishing. Scaffolding using previous linkage map data allowed us to reconstruct a chromosome level assembly with high completeness, complemented with gene annotation and repeat masking. The 696 Mb long assembly has an N50 = 27.87 Mb and an L50 = 12, with 92.85% of its length placed in 24 chromosomes. We use this new resource to study the evolution of the meagre genome and other Sciaenids, via a comparative analysis of 25 high-quality teleost genomes. Combining a rigorous investigation of gene duplications with base-wise conservation analysis, we identify candidate loci related to immune, fat metabolism and growth adaptations in the meagre. Following phylogenomic reconstruction, we show highly conserved synteny within Sciaenidae. In contrast, we report rapidly evolving syntenic rearrangements and gene copy changes in the sex-related dmrt1 neighbourhood in meagre and other members of the family. These novel genomic datasets and findings will add important new tools for aquaculture studies and greatly facilitate husbandry and breeding work in the species. |
Valdivieso, Alejandro; Sánchez-Baizán, Núria; Mitrizakis, Nikos; Papandroulakis, Nikos; Piferrer, Francesc Aquaculture, 563 , pp. 738918, 2023, ISSN: 00448486. @article{valdivieso_development_2023, title = {Development of epigenetic biomarkers with diagnostic and prognostic value to assess the lasting effects of early temperature changes in farmed fish}, author = {Alejandro Valdivieso and Núria Sánchez-Baizán and Nikos Mitrizakis and Nikos Papandroulakis and Francesc Piferrer}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/11/2023-Valdivieso-3.pdf https://linkinghub.elsevier.com/retrieve/pii/S0044848622010353}, doi = {10.1016/j.aquaculture.2022.738918}, issn = {00448486}, year = {2023}, date = {2023-01-04}, urldate = {2022-11-10}, journal = {Aquaculture}, volume = {563}, pages = {738918}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Rigos, George; Kogiannou, Dimitra Antimicrobial drugs in aquaculture: use and abuse Incollection Present Knowledge in Food Safety, pp. 142–161, Elsevier, 2023, ISBN: 9780128194706. @incollection{rigos_antimicrobial_2023, title = {Antimicrobial drugs in aquaculture: use and abuse}, author = {George Rigos and Dimitra Kogiannou}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/10/2022-Rigos-preprint-72.pdf https://linkinghub.elsevier.com/retrieve/pii/B9780128194706000275}, doi = {10.1016/B978-0-12-819470-6.00027-5}, isbn = {9780128194706}, year = {2023}, date = {2023-01-02}, urldate = {2022-10-24}, booktitle = {Present Knowledge in Food Safety}, pages = {142--161}, publisher = {Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {incollection} } |
Samaras, Athanasios; Tsoukali, Panagiota; Katsika, Lydia; Pavlidis, Michail; Papadakis, Ioannis E Aquaculture, 562 , pp. 738830, 2023, ISSN: 00448486. @article{samaras_chronic_2023, title = {Chronic impact of exposure to low dissolved oxygen on the physiology of Dicentrarchus labrax and Sparus aurata and its effects on the acute stress response}, author = {Athanasios Samaras and Panagiota Tsoukali and Lydia Katsika and Michail Pavlidis and Ioannis E Papadakis}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/10/2022-Samaras-AQUA-66-preprint-1.pdf https://linkinghub.elsevier.com/retrieve/pii/S0044848622009474}, doi = {10.1016/j.aquaculture.2022.738830}, issn = {00448486}, year = {2023}, date = {2023-01-01}, urldate = {2022-10-12}, journal = {Aquaculture}, volume = {562}, pages = {738830}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2022 |
Panteli, Nikolas; Demertzioglou, Maria; Feidantsis, Konstantinos; Karapanagiotis, Stelios; Tsele, Nikoletta; Tsakoniti, Kalliopi; Gkagkavouzis, Konstantinos; Mylonas, Constantinos C; Kormas, Konstantinos Ar.; Mente, Eleni; Antonopoulou, Efthimia Fish Physiology and Biochemistry, 48 (6), pp. 1665–1684, 2022, ISSN: 0920-1742, 1573-5168. @article{panteli_advances_2022, title = {Advances in understanding the mitogenic, metabolic, and cell death signaling in teleost development: the case of greater amberjack (Seriola dumerili, Risso 1810)}, author = {Nikolas Panteli and Maria Demertzioglou and Konstantinos Feidantsis and Stelios Karapanagiotis and Nikoletta Tsele and Kalliopi Tsakoniti and Konstantinos Gkagkavouzis and Constantinos C Mylonas and Konstantinos Ar. Kormas and Eleni Mente and Efthimia Antonopoulou}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/12/2022-Panteli-FPB-preprint-80.pdf https://link.springer.com/10.1007/s10695-022-01146-5}, doi = {10.1007/s10695-022-01146-5}, issn = {0920-1742, 1573-5168}, year = {2022}, date = {2022-12-21}, urldate = {2022-12-21}, journal = {Fish Physiology and Biochemistry}, volume = {48}, number = {6}, pages = {1665--1684}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Montesanto, Federica; Albano, Marco; Ayas, Dενιζ; Betti, Federico; Capillo, Gioele; Çinar, Melih Ertan; Corsini-Foka, Maria; Crocetta, Fabio; Dağli, Ertan; D’Iglio, Claudio; Digenis, Markos; Dragičević, Branko; Famulari, Sergio; Ergüden, Deniz; Giova, Antonio; Giussani, Valentina; Hoffman, Razy; Isajlović, Igor; Lipej, Lovrenc; López-Esclapez, Raquel; Mastrototaro, Francesco; Moreni, Alessandra; Orenes-Salazar, Victor; Ovalis, Panayotis Ovalis Panayotis; Plaiti, Wanda; Pujol, Juan A; Rabaoui, Lotfi; Rallis, Ioannis; Rogelja, Manja; Savoca, Serena; Skouradakis, Grigorios; Tiralongo, Francesco; Toma, Margherita; Trkov, Domen; Ubero-Pascal, Nicolas; Yacoubi, Lamia; Yalgin, Ferhat; Yapici, Sercan; Zamuda, Leon L New records of rare species in the Mediterranean Sea (December 2022) Journal Article Mediterranean Marine Science, 23 (4), pp. 968–994, 2022, ISSN: 1791-6763, 1108-393X. @article{montesanto_new_2022, title = {New records of rare species in the Mediterranean Sea (December 2022)}, author = {Federica Montesanto and Marco Albano and Dενιζ Ayas and Federico Betti and Gioele Capillo and Melih Ertan Çinar and Maria Corsini-Foka and Fabio Crocetta and Ertan Dağli and Claudio D’Iglio and Markos Digenis and Branko Dragičević and Sergio Famulari and Deniz Ergüden and Antonio Giova and Valentina Giussani and Razy Hoffman and Igor Isajlović and Lovrenc Lipej and Raquel López-Esclapez and Francesco Mastrototaro and Alessandra Moreni and Victor Orenes-Salazar and Panayotis Ovalis Panayotis Ovalis and Wanda Plaiti and Juan A Pujol and Lotfi Rabaoui and Ioannis Rallis and Manja Rogelja and Serena Savoca and Grigorios Skouradakis and Francesco Tiralongo and Margherita Toma and Domen Trkov and Nicolas Ubero-Pascal and Lamia Yacoubi and Ferhat Yalgin and Sercan Yapici and Leon L Zamuda}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/12/2022-Montesanto-MMS-79.pdf https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/32369}, doi = {10.12681/mms.32369}, issn = {1791-6763, 1108-393X}, year = {2022}, date = {2022-12-19}, urldate = {2022-12-20}, journal = {Mediterranean Marine Science}, volume = {23}, number = {4}, pages = {968--994}, abstract = {This Collective Article presents information on 20 taxa belonging to five (5) Phyla: Cnidaria (2), Mollusca (8), Arthropoda (4), Echinodermata (1) and Chordata (5) recorded from the Alboran Sea to the Levantine Sea. These new records were found in seven (7) different ecoregions as follows: Alboran Sea: new record of the rare football octopus Ocythoe tuberculata in the harbour of Algeciras (Spain); Western Mediterranean Sea: first record of the isopod Arcturinella deltensis in the Ligurian Sea, and the third in the whole Mediterranean Sea; third Mediterranean site for the Muricidae Coralliophila ahuiri along the Italian coasts (Alghero); first and westernmost record of the goby Corcyrogobius liechtensteini from the Iberian Peninsula; new record of the sea slug Glaucus atlanticus from the coast of the Iberian Peninsula; first and easternmost record of Holothuria (Vaneyothuria) lentiginosa lentiginosa along the Italian coasts; Tunisian Plateau/Gulf of Sidra: seventh record of the oceanic squid Thysanoteuthis rhombus in Tunisia, and first for the Gulf of Gabes. Ionian Sea: second occurrence of the Atlantic tripletail Lobotes surinamensis in the Ionian Sea and first record from the Strait of Messina area; new record of the rare sea elephant Pterotrachea coronata from the Strait of Messina; Adriatic Sea: first record of a facies of football ascidian Diazona violacea in the South Adriatic Sea (Tremiti Island, Italy); two records of the sevengill shark Heptranchias perlo in Croatian waters after more than 70 years of absence of documented records in this area; first known occurrence of the nudibranch Jalonus hyalinus in Slovenian waters and also the first known occurrence of this species in the Adriatic Sea; first record of the nudibranch Okenia elegans in Slovenian waters; Aegean Sea: First record of the parasitic isopod Nerocila milesensis along Greek coasts (Crete island) as well as the second record worldwide; additional record of the brachyuran Paragalene longicrura collected from the Saronikos Gulf (Greece); first record of the siphonophore Rhizophysa filiformis in Greek waters as well as the second record of this species in the eastern Mediterranean basin; new record of the rare and protected angelshark Squatina aculeata along Turkish coasts; Levantine Sea: first record of the marine amphipod Caprella andreae in the Levantine Mediterranean shore of Israel, based on both morphological and molecular data; first occurrence of the cephalopod Tremoctopus violaceus along Turkish coasts, which confirms its presence in the north-eastern coasts of Turkey; record of a bloom of the thermophilic jellyfish Pelagia noctiluca in the north Levantine Sea.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This Collective Article presents information on 20 taxa belonging to five (5) Phyla: Cnidaria (2), Mollusca (8), Arthropoda (4), Echinodermata (1) and Chordata (5) recorded from the Alboran Sea to the Levantine Sea. These new records were found in seven (7) different ecoregions as follows: Alboran Sea: new record of the rare football octopus Ocythoe tuberculata in the harbour of Algeciras (Spain); Western Mediterranean Sea: first record of the isopod Arcturinella deltensis in the Ligurian Sea, and the third in the whole Mediterranean Sea; third Mediterranean site for the Muricidae Coralliophila ahuiri along the Italian coasts (Alghero); first and westernmost record of the goby Corcyrogobius liechtensteini from the Iberian Peninsula; new record of the sea slug Glaucus atlanticus from the coast of the Iberian Peninsula; first and easternmost record of Holothuria (Vaneyothuria) lentiginosa lentiginosa along the Italian coasts; Tunisian Plateau/Gulf of Sidra: seventh record of the oceanic squid Thysanoteuthis rhombus in Tunisia, and first for the Gulf of Gabes. Ionian Sea: second occurrence of the Atlantic tripletail Lobotes surinamensis in the Ionian Sea and first record from the Strait of Messina area; new record of the rare sea elephant Pterotrachea coronata from the Strait of Messina; Adriatic Sea: first record of a facies of football ascidian Diazona violacea in the South Adriatic Sea (Tremiti Island, Italy); two records of the sevengill shark Heptranchias perlo in Croatian waters after more than 70 years of absence of documented records in this area; first known occurrence of the nudibranch Jalonus hyalinus in Slovenian waters and also the first known occurrence of this species in the Adriatic Sea; first record of the nudibranch Okenia elegans in Slovenian waters; Aegean Sea: First record of the parasitic isopod Nerocila milesensis along Greek coasts (Crete island) as well as the second record worldwide; additional record of the brachyuran Paragalene longicrura collected from the Saronikos Gulf (Greece); first record of the siphonophore Rhizophysa filiformis in Greek waters as well as the second record of this species in the eastern Mediterranean basin; new record of the rare and protected angelshark Squatina aculeata along Turkish coasts; Levantine Sea: first record of the marine amphipod Caprella andreae in the Levantine Mediterranean shore of Israel, based on both morphological and molecular data; first occurrence of the cephalopod Tremoctopus violaceus along Turkish coasts, which confirms its presence in the north-eastern coasts of Turkey; record of a bloom of the thermophilic jellyfish Pelagia noctiluca in the north Levantine Sea. |
Pavloudi, Christina; Zafeiropoulos, Haris Deciphering the community structure and the functional potential of a hypersaline marsh microbial mat community Journal Article FEMS Microbiology Ecology, 98 (12), pp. fiac141, 2022, ISSN: 1574-6941. @article{pavloudi_deciphering_2022, title = {Deciphering the community structure and the functional potential of a hypersaline marsh microbial mat community}, author = {Christina Pavloudi and Haris Zafeiropoulos}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/12/2022-Pavloudi-Zafeiropoulos-FEMS-preprint.pdf https://academic.oup.com/femsec/article/doi/10.1093/femsec/fiac141/6843573}, doi = {10.1093/femsec/fiac141}, issn = {1574-6941}, year = {2022}, date = {2022-12-12}, urldate = {2022-12-13}, journal = {FEMS Microbiology Ecology}, volume = {98}, number = {12}, pages = {fiac141}, abstract = {Abstract Microbial mats are vertically stratified communities of microorganisms characterized by pronounced physiochemical gradients allowing for high species diversity and a wide range of metabolic capabilities. High Throughput Sequencing has the potential to reveal the biodiversity and function of such ecosystems in the cycling of elements. The present study combines 16S rRNA amplicon sequencing and shotgun metagenomics on a hypersaline marsh in Tristomo bay (Karpathos, Greece). Samples were collected in July 2018 and November 2019 from microbial mats, deeper sediment, aggregates observed in the water overlying the sediment, as well as sediment samples with no apparent layering. Metagenomic samples’ coassembly and binning revealed 250 bacterial and 39 archaeal metagenome-assembled genomes, with completeness estimates higher than 70% and contamination less than 5%. All MAGs had KEGG Orthology terms related to osmoadaptation, with the ‘salt in’ strategy ones being prominent. Halobacteria and Bacteroidetes were the most abundant taxa in the mats. Photosynthesis was most likely performed by purple sulphur and nonsulphur bacteria. All samples had the capacity for sulphate reduction, dissimilatory arsenic reduction, and conversion of pyruvate to oxaloacetate. Overall, both sequencing methodologies resulted in similar taxonomic compositions and revealed that the formation of the microbial mat in this marsh exhibits seasonal variation.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Microbial mats are vertically stratified communities of microorganisms characterized by pronounced physiochemical gradients allowing for high species diversity and a wide range of metabolic capabilities. High Throughput Sequencing has the potential to reveal the biodiversity and function of such ecosystems in the cycling of elements. The present study combines 16S rRNA amplicon sequencing and shotgun metagenomics on a hypersaline marsh in Tristomo bay (Karpathos, Greece). Samples were collected in July 2018 and November 2019 from microbial mats, deeper sediment, aggregates observed in the water overlying the sediment, as well as sediment samples with no apparent layering. Metagenomic samples’ coassembly and binning revealed 250 bacterial and 39 archaeal metagenome-assembled genomes, with completeness estimates higher than 70% and contamination less than 5%. All MAGs had KEGG Orthology terms related to osmoadaptation, with the ‘salt in’ strategy ones being prominent. Halobacteria and Bacteroidetes were the most abundant taxa in the mats. Photosynthesis was most likely performed by purple sulphur and nonsulphur bacteria. All samples had the capacity for sulphate reduction, dissimilatory arsenic reduction, and conversion of pyruvate to oxaloacetate. Overall, both sequencing methodologies resulted in similar taxonomic compositions and revealed that the formation of the microbial mat in this marsh exhibits seasonal variation. |
Mancinelli, Giorgio; Dailianis, Thanos; Dounas, Costas; Kasapidis, Panagiotis; Koulouri, Panayota; Skouradakis, Grigorios; Bardelli, Roberta; Muri, Cristina Di; Guerra, Maria Teresa; Vizzini, Salvatrice Sustainability, 14 (22), pp. 15202, 2022, ISSN: 2071-1050. @article{mancinelli_isotopic_2022, title = {Isotopic Niche and Trophic Position of the Invasive Portunid Portunus segnis Forskål, (1775) in Elounda Bay (Crete Island, Eastern Mediterranean)}, author = {Giorgio Mancinelli and Thanos Dailianis and Costas Dounas and Panagiotis Kasapidis and Panayota Koulouri and Grigorios Skouradakis and Roberta Bardelli and Cristina Di Muri and Maria Teresa Guerra and Salvatrice Vizzini}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/11/2022-Mancinelli-Sustain-77.pdf https://www.mdpi.com/2071-1050/14/22/15202}, doi = {10.3390/su142215202}, issn = {2071-1050}, year = {2022}, date = {2022-11-21}, urldate = {2022-11-21}, journal = {Sustainability}, volume = {14}, number = {22}, pages = {15202}, abstract = {There is a growing recognition that an advanced understanding of the trophic characteristics of an invasive consumer can provide important information on its ecological impact. In recent years, the blue swimming crab Portunus segnis, one of the earliest Lessepsian invaders, has considerably expanded its distribution range in the Mediterranean Sea, yet, its trophic habits in invaded areas remain scarcely investigated. In this study, we used carbon and nitrogen stable isotopes analysis (SIA) to determine the trophic position and isotopic niche of the crab compared with other representatives of the flora and fauna occurring in Elounda Bay (Crete). P. segnis showed a trophic position of 3.9, higher than the values determined by SIA or conventional gut content analysis in other areas of the Mediterranean Sea or in the native range. Crab specimens showed a high inter-individual variability in both δ13C and δ15N values; further analysis indicated negligible differences in the isotopic niche of adult males and females. Conversely, δ15N values were significantly related to the size of the specimens, ultimately suggesting an ontogenetic dietary shift. This study represents the first attempt to characterize the trophic habits of the blue swimming crab in the context of an invaded food web and may contribute to the implementation of long-term management strategies of control and mitigation of its ecological impact.}, keywords = {}, pubstate = {published}, tppubtype = {article} } There is a growing recognition that an advanced understanding of the trophic characteristics of an invasive consumer can provide important information on its ecological impact. In recent years, the blue swimming crab Portunus segnis, one of the earliest Lessepsian invaders, has considerably expanded its distribution range in the Mediterranean Sea, yet, its trophic habits in invaded areas remain scarcely investigated. In this study, we used carbon and nitrogen stable isotopes analysis (SIA) to determine the trophic position and isotopic niche of the crab compared with other representatives of the flora and fauna occurring in Elounda Bay (Crete). P. segnis showed a trophic position of 3.9, higher than the values determined by SIA or conventional gut content analysis in other areas of the Mediterranean Sea or in the native range. Crab specimens showed a high inter-individual variability in both δ13C and δ15N values; further analysis indicated negligible differences in the isotopic niche of adult males and females. Conversely, δ15N values were significantly related to the size of the specimens, ultimately suggesting an ontogenetic dietary shift. This study represents the first attempt to characterize the trophic habits of the blue swimming crab in the context of an invaded food web and may contribute to the implementation of long-term management strategies of control and mitigation of its ecological impact. |
Chatziantoniou, Andromachi; Spondylidis, Spyros Charalampis; Stavrakidis-Zachou, Orestis; Papandroulakis, Nikos; Topouzelis, Konstantinos Dissolved oxygen estimation in aquaculture sites using remote sensing and machine learning Journal Article Remote Sensing Applications: Society and Environment, 28 , pp. 100865, 2022, ISSN: 23529385. @article{chatziantoniou_dissolved_2022, title = {Dissolved oxygen estimation in aquaculture sites using remote sensing and machine learning}, author = {Andromachi Chatziantoniou and Spyros Charalampis Spondylidis and Orestis Stavrakidis-Zachou and Nikos Papandroulakis and Konstantinos Topouzelis}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/11/2022-Chatziantoniou-RSA-76.pdf https://linkinghub.elsevier.com/retrieve/pii/S2352938522001732}, doi = {10.1016/j.rsase.2022.100865}, issn = {23529385}, year = {2022}, date = {2022-11-18}, urldate = {2022-11-18}, journal = {Remote Sensing Applications: Society and Environment}, volume = {28}, pages = {100865}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ramos-Júdez, Sandra; Danis, Theodoros; Angelova, Nelina; Tsakogiannis, Alexandros; Giménez, Ignacio; Tsigenopoulos, Costas S; Duncan, Neil; Manousaki, Tereza Transcriptome analysis of flathead grey mullet (Mugil cephalus) ovarian development induced by recombinant gonadotropin hormones Journal Article Frontiers in Physiology, 13 , pp. 1033445, 2022, ISSN: 1664-042X. @article{ramos-judez_transcriptome_2022, title = {Transcriptome analysis of flathead grey mullet (Mugil cephalus) ovarian development induced by recombinant gonadotropin hormones}, author = {Sandra Ramos-Júdez and Theodoros Danis and Nelina Angelova and Alexandros Tsakogiannis and Ignacio Giménez and Costas S Tsigenopoulos and Neil Duncan and Tereza Manousaki}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/11/2022-Ramos-Judez-fphys-75-1.pdf https://www.frontiersin.org/articles/10.3389/fphys.2022.1033445/full}, doi = {10.3389/fphys.2022.1033445}, issn = {1664-042X}, year = {2022}, date = {2022-11-14}, urldate = {2022-11-14}, journal = {Frontiers in Physiology}, volume = {13}, pages = {1033445}, abstract = {Background: Treatment with recombinant gonadotropin hormones (rGths), follicle-stimulating hormone (rFsh) and luteinizing hormone (rLh), was shown to induce and complete vitellogenesis to finally obtain viable eggs and larvae in the flathead grey mullet ( Mugil cephalus ), a teleost arrested at early stages of gametogenesis in intensive captivity conditions. This study aimed to investigate the transcriptomic changes that occur in the ovary of females during the rGths-induced vitellogenesis. Methods: Ovarian samples were collected through biopsies from the same five females at four stages of ovarian development. RNASeq libraries were constructed for all stages studied, sequenced on an Illumina HiSeq4000, and a de novo transcriptome was constructed. Differentially expressed genes (DEGs) were identified between stages and the functional properties of DEGs were characterized by comparison with the gene ontology and Kyoto Encyclopedia. An enrichment analysis of molecular pathways was performed. Results: The de novo transcriptome comprised 287,089 transcripts after filtering. As vitellogenesis progressed, more genes were significantly upregulated than downregulated. The rFsh application induced ovarian development from previtellogenesis to early-to-mid-vitellogenesis with associated pathways enriched from upregulated DEGs related to ovarian steroidogenesis and reproductive development , cholesterol metabolism, ovarian growth and differentiation, lipid accumulation, and cell-to-cell adhesion pathways. The application of rFsh and rLh at early-to-mid-vitellogenesis induced the growth of oocytes to late-vitellogenesis and, with it, the enrichment of pathways from upregulated DEGs related to the production of energy, such as the lysosomes activity . The application of rLh at late-vitellogenesis induced the completion of vitellogenesis with the enrichment of pathways linked with the switch from vitellogenesis to oocyte maturation. Conclusion: The DEGs and enriched molecular pathways described during the induced vitellogenesis of flathead grey mullet with rGths were typical of natural oogenesis reported for other fish species. Present results add new knowledge to the rGths action to further raise the possibility of using rGths in species that present similar reproductive disorders in aquaculture, the aquarium industry as well as the conservation of endangered species.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Background: Treatment with recombinant gonadotropin hormones (rGths), follicle-stimulating hormone (rFsh) and luteinizing hormone (rLh), was shown to induce and complete vitellogenesis to finally obtain viable eggs and larvae in the flathead grey mullet ( Mugil cephalus ), a teleost arrested at early stages of gametogenesis in intensive captivity conditions. This study aimed to investigate the transcriptomic changes that occur in the ovary of females during the rGths-induced vitellogenesis. Methods: Ovarian samples were collected through biopsies from the same five females at four stages of ovarian development. RNASeq libraries were constructed for all stages studied, sequenced on an Illumina HiSeq4000, and a de novo transcriptome was constructed. Differentially expressed genes (DEGs) were identified between stages and the functional properties of DEGs were characterized by comparison with the gene ontology and Kyoto Encyclopedia. An enrichment analysis of molecular pathways was performed. Results: The de novo transcriptome comprised 287,089 transcripts after filtering. As vitellogenesis progressed, more genes were significantly upregulated than downregulated. The rFsh application induced ovarian development from previtellogenesis to early-to-mid-vitellogenesis with associated pathways enriched from upregulated DEGs related to ovarian steroidogenesis and reproductive development , cholesterol metabolism, ovarian growth and differentiation, lipid accumulation, and cell-to-cell adhesion pathways. The application of rFsh and rLh at early-to-mid-vitellogenesis induced the growth of oocytes to late-vitellogenesis and, with it, the enrichment of pathways from upregulated DEGs related to the production of energy, such as the lysosomes activity . The application of rLh at late-vitellogenesis induced the completion of vitellogenesis with the enrichment of pathways linked with the switch from vitellogenesis to oocyte maturation. Conclusion: The DEGs and enriched molecular pathways described during the induced vitellogenesis of flathead grey mullet with rGths were typical of natural oogenesis reported for other fish species. Present results add new knowledge to the rGths action to further raise the possibility of using rGths in species that present similar reproductive disorders in aquaculture, the aquarium industry as well as the conservation of endangered species. |
Papanagnou, Eleni‐Dimitra; Gumeni, Sentiljana; Sklirou, Aimilia D; Rafeletou, Alexandra; Terpos, Evangelos; Keklikoglou, Kleoniki; Kastritis, Efstathios; Stamatelopoulos, Kimon; Sykiotis, Gerasimos P; Dimopoulos, Meletios A; Trougakos, Ioannis P Autophagy activation can partially rescue proteasome dysfunction‐mediated cardiac toxicity Journal Article Aging Cell, 2022, ISSN: 1474-9718, 1474-9726. @article{papanagnou_autophagy_2022, title = {Autophagy activation can partially rescue proteasome dysfunction‐mediated cardiac toxicity}, author = {Eleni‐Dimitra Papanagnou and Sentiljana Gumeni and Aimilia D Sklirou and Alexandra Rafeletou and Evangelos Terpos and Kleoniki Keklikoglou and Efstathios Kastritis and Kimon Stamatelopoulos and Gerasimos P Sykiotis and Meletios A Dimopoulos and Ioannis P Trougakos}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/11/2022-Papanagnou-Keklikoglou-74.pdf https://onlinelibrary.wiley.com/doi/10.1111/acel.13715}, doi = {10.1111/acel.13715}, issn = {1474-9718, 1474-9726}, year = {2022}, date = {2022-11-07}, urldate = {2022-11-07}, journal = {Aging Cell}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Magneville, Camille; Bricquir, Marie‐Lou Leréec Le; Dailianis, Thanos; Skouradakis, Grigorios; Claverie, Thomas; Villéger, Sébastien Long‐duration remote underwater videos reveal that grazing by fishes is highly variable through time and dominated by non‐indigenous species Journal Article Remote Sensing in Ecology and Conservation, pp. rse2.311, 2022, ISSN: 2056-3485, 2056-3485. @article{magneville_longduration_2022, title = {Long‐duration remote underwater videos reveal that grazing by fishes is highly variable through time and dominated by non‐indigenous species}, author = {Camille Magneville and Marie‐Lou Leréec Le Bricquir and Thanos Dailianis and Grigorios Skouradakis and Thomas Claverie and Sébastien Villéger}, editor = {Kylie Scales and Jacquomo Monk}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/11/2022-Magneville-Remote-Sens-Ecol-Conserv-73.pdf https://onlinelibrary.wiley.com/doi/10.1002/rse2.311}, doi = {10.1002/rse2.311}, issn = {2056-3485, 2056-3485}, year = {2022}, date = {2022-11-04}, urldate = {2022-11-04}, journal = {Remote Sensing in Ecology and Conservation}, pages = {rse2.311}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Keith, David A; Ferrer-Paris, José R; Nicholson, Emily; Bishop, Melanie J; Polidoro, Beth A; Ramirez-Llodra, Eva; Tozer, Mark G; Nel, Jeanne L; Nally, Ralph Mac; Gregr, Edward J; Watermeyer, Kate E; Essl, Franz; Faber-Langendoen, Don; Franklin, Janet; Lehmann, Caroline E R; Etter, Andrés; Roux, Dirk J; Stark, Jonathan S; Rowland, Jessica A; Brummitt, Neil A; Fernandez-Arcaya, Ulla C; Suthers, Iain M; Wiser, Susan K; Donohue, Ian; Jackson, Leland J; Pennington, Toby R; Iliffe, Thomas M; Gerovasileiou, Vasilis; Giller, Paul; Robson, Belinda J; Pettorelli, Nathalie; Andrade, Angela; Lindgaard, Arild; Tahvanainen, Teemu; Terauds, Aleks; Chadwick, Michael A; Murray, Nicholas J; Moat, Justin; Pliscoff, Patricio; Zager, Irene; Kingsford, Richard T A function-based typology for Earth’s ecosystems Journal Article Nature, 610 (7932), pp. 513–518, 2022, ISSN: 0028-0836, 1476-4687. @article{keith_function-based_2022, title = {A function-based typology for Earth’s ecosystems}, author = {David A Keith and José R Ferrer-Paris and Emily Nicholson and Melanie J Bishop and Beth A Polidoro and Eva Ramirez-Llodra and Mark G Tozer and Jeanne L Nel and Ralph Mac Nally and Edward J Gregr and Kate E Watermeyer and Franz Essl and Don Faber-Langendoen and Janet Franklin and Caroline E R Lehmann and Andrés Etter and Dirk J Roux and Jonathan S Stark and Jessica A Rowland and Neil A Brummitt and Ulla C Fernandez-Arcaya and Iain M Suthers and Susan K Wiser and Ian Donohue and Leland J Jackson and Toby R Pennington and Thomas M Iliffe and Vasilis Gerovasileiou and Paul Giller and Belinda J Robson and Nathalie Pettorelli and Angela Andrade and Arild Lindgaard and Teemu Tahvanainen and Aleks Terauds and Michael A Chadwick and Nicholas J Murray and Justin Moat and Patricio Pliscoff and Irene Zager and Richard T Kingsford}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/11/2022-Keith-NATURE-72.pdf https://www.nature.com/articles/s41586-022-05318-4}, doi = {10.1038/s41586-022-05318-4}, issn = {0028-0836, 1476-4687}, year = {2022}, date = {2022-11-02}, urldate = {2022-11-02}, journal = {Nature}, volume = {610}, number = {7932}, pages = {513--518}, abstract = {Abstract As the United Nations develops a post-2020 global biodiversity framework for the Convention on Biological Diversity, attention is focusing on how new goals and targets for ecosystem conservation might serve its vision of ‘living in harmony with nature’ 1,2 . Advancing dual imperatives to conserve biodiversity and sustain ecosystem services requires reliable and resilient generalizations and predictions about ecosystem responses to environmental change and management 3 . Ecosystems vary in their biota 4 , service provision 5 and relative exposure to risks 6 , yet there is no globally consistent classification of ecosystems that reflects functional responses to change and management. This hampers progress on developing conservation targets and sustainability goals. Here we present the International Union for Conservation of Nature (IUCN) Global Ecosystem Typology, a conceptually robust, scalable, spatially explicit approach for generalizations and predictions about functions, biota, risks and management remedies across the entire biosphere. The outcome of a major cross-disciplinary collaboration, this novel framework places all of Earth’s ecosystems into a unifying theoretical context to guide the transformation of ecosystem policy and management from global to local scales. This new information infrastructure will support knowledge transfer for ecosystem-specific management and restoration, globally standardized ecosystem risk assessments, natural capital accounting and progress on the post-2020 global biodiversity framework.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract As the United Nations develops a post-2020 global biodiversity framework for the Convention on Biological Diversity, attention is focusing on how new goals and targets for ecosystem conservation might serve its vision of ‘living in harmony with nature’ 1,2 . Advancing dual imperatives to conserve biodiversity and sustain ecosystem services requires reliable and resilient generalizations and predictions about ecosystem responses to environmental change and management 3 . Ecosystems vary in their biota 4 , service provision 5 and relative exposure to risks 6 , yet there is no globally consistent classification of ecosystems that reflects functional responses to change and management. This hampers progress on developing conservation targets and sustainability goals. Here we present the International Union for Conservation of Nature (IUCN) Global Ecosystem Typology, a conceptually robust, scalable, spatially explicit approach for generalizations and predictions about functions, biota, risks and management remedies across the entire biosphere. The outcome of a major cross-disciplinary collaboration, this novel framework places all of Earth’s ecosystems into a unifying theoretical context to guide the transformation of ecosystem policy and management from global to local scales. This new information infrastructure will support knowledge transfer for ecosystem-specific management and restoration, globally standardized ecosystem risk assessments, natural capital accounting and progress on the post-2020 global biodiversity framework. |
Mallioris, Panagiotis; Kotzamanis, Yannis; Vardali, Sofia; Roussos, Efstratios; Ilia, Vasiliki; Paschali, Elissavet; Kampantais, Dimitrios; Bouziotis, Dimitrios; Khadem, Alireza; Vatsos, Ioannis N Animal Feed Science and Technology, 284 , pp. 115194, 2022, ISSN: 03778401. @article{mallioris_modulation_2022, title = {Modulation of intestinal health and hepatic vacuolation in gilthead sea bream (Sparus aurata) juveniles by a mixture of dietary esterified butyrins, emulsifiers from plants and yeast extracts at low and high fish meal inclusion}, author = {Panagiotis Mallioris and Yannis Kotzamanis and Sofia Vardali and Efstratios Roussos and Vasiliki Ilia and Elissavet Paschali and Dimitrios Kampantais and Dimitrios Bouziotis and Alireza Khadem and Ioannis N Vatsos}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0377840121003801}, doi = {10.1016/j.anifeedsci.2021.115194}, issn = {03778401}, year = {2022}, date = {2022-10-26}, urldate = {2022-10-26}, journal = {Animal Feed Science and Technology}, volume = {284}, pages = {115194}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Henry, M A; Golomazou, E; Asimaki, A; Psofakis, P; Fountoulaki, E; Mente, E; Rumbos, C I; Athanassiou, C G; Karapanagiotidis, I T Aquaculture Reports, 27 , pp. 101347, 2022, ISSN: 23525134. @article{henry_partial_2022, title = {Partial dietary fishmeal replacement with full-fat or defatted superworm (Zophobas morio) larvae meals modulates the innate immune system of gilthead seabream, Sparus aurata}, author = {M A Henry and E Golomazou and A Asimaki and P Psofakis and E Fountoulaki and E Mente and C I Rumbos and C G Athanassiou and I T Karapanagiotidis}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/10/2022-Henry-AquaRepo-66.pdf https://linkinghub.elsevier.com/retrieve/pii/S235251342200343X}, doi = {10.1016/j.aqrep.2022.101347}, issn = {23525134}, year = {2022}, date = {2022-10-19}, urldate = {2022-10-19}, journal = {Aquaculture Reports}, volume = {27}, pages = {101347}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Mastoraki, Maria; Katsika, Lydia; Enes, Paula; Guerreiro, Inês; Kotzamanis, Yannis P; Gasco, Laura; Chatzifotis, Stavros; Antonopoulou, Efthimia Aquaculture, 561 , pp. 738674, 2022, ISSN: 00448486. @article{mastoraki_insect_2022, title = {Insect meals in feeds for juvenile gilthead seabream (Sparus aurata): Effects on growth, blood chemistry, hepatic metabolic enzymes, body composition and nutrient utilization}, author = {Maria Mastoraki and Lydia Katsika and Paula Enes and Inês Guerreiro and Yannis P Kotzamanis and Laura Gasco and Stavros Chatzifotis and Efthimia Antonopoulou}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/10/2022-Mastoraki-AQUApre-print-70.pdf https://linkinghub.elsevier.com/retrieve/pii/S0044848622007918}, doi = {10.1016/j.aquaculture.2022.738674}, issn = {00448486}, year = {2022}, date = {2022-10-14}, urldate = {2022-10-14}, journal = {Aquaculture}, volume = {561}, pages = {738674}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Garrabou, Joaquim; Gómez‐Gras, Daniel; Medrano, Alba; Cerrano, Carlo; Ponti, Massimo; Schlegel, Robert; Bensoussan, Nathaniel; Turicchia, Eva; Sini, Maria; Gerovasileiou, Vasilis; Teixido, Nuria; Mirasole, Alice; Tamburello, Laura; Cebrian, Emma; Rilov, Gil; Ledoux, Jean‐Baptiste; Souissi, Jamila Ben; Khamassi, Faten; Ghanem, Raouia; Benabdi, Mouloud; Grimes, Samir; Ocaña, Oscar; Bazairi, Hocein; Hereu, Bernat; Linares, Cristina; Kersting, Diego Kurt; la Rovira, Graciel; Ortega, Júlia; Casals, David; Pagès‐Escolà, Marta; Margarit, Núria; Capdevila, Pol; Verdura, Jana; Ramos, Alfonso; Izquierdo, Andres; Barbera, Carmen; Rubio‐Portillo, Esther; Anton, Irene; López‐Sendino, Paula; Díaz, David; Vázquez‐Luis, Maite; Duarte, Carlos; Marbà, Nuria; Aspillaga, Eneko; Espinosa, Free; Grech, Daniele; Guala, Ivan; Azzurro, Ernesto; Farina, Simone; Gambi, Maria Cristina; Chimienti, Giovanni; Montefalcone, Monica; Azzola, Annalisa; Mantas, Torcuato Pulido; Fraschetti, Simonetta; Ceccherelli, Giulia; Kipson, Silvija; Bakran‐Petricioli, Tatjana; Petricioli, Donat; Jimenez, Carlos; Katsanevakis, Stelios; Kizilkaya, Inci Tuney; Kizilkaya, Zafer; Sartoretto, Stephane; Elodie, Rouanet; Ruitton, Sandrine; Comeau, Steeve; Gattuso, Jean‐Pierre; Harmelin, Jean‐Georges Marine heatwaves drive recurrent mass mortalities in the Mediterranean Sea Journal Article Global Change Biology, 28 (19), pp. 5708–5725, 2022, ISSN: 1354-1013, 1365-2486. @article{garrabou_marine_2022, title = {Marine heatwaves drive recurrent mass mortalities in the Mediterranean Sea}, author = {Joaquim Garrabou and Daniel Gómez‐Gras and Alba Medrano and Carlo Cerrano and Massimo Ponti and Robert Schlegel and Nathaniel Bensoussan and Eva Turicchia and Maria Sini and Vasilis Gerovasileiou and Nuria Teixido and Alice Mirasole and Laura Tamburello and Emma Cebrian and Gil Rilov and Jean‐Baptiste Ledoux and Jamila Ben Souissi and Faten Khamassi and Raouia Ghanem and Mouloud Benabdi and Samir Grimes and Oscar Ocaña and Hocein Bazairi and Bernat Hereu and Cristina Linares and Diego Kurt Kersting and Graciel la Rovira and Júlia Ortega and David Casals and Marta Pagès‐Escolà and Núria Margarit and Pol Capdevila and Jana Verdura and Alfonso Ramos and Andres Izquierdo and Carmen Barbera and Esther Rubio‐Portillo and Irene Anton and Paula López‐Sendino and David Díaz and Maite Vázquez‐Luis and Carlos Duarte and Nuria Marbà and Eneko Aspillaga and Free Espinosa and Daniele Grech and Ivan Guala and Ernesto Azzurro and Simone Farina and Maria Cristina Gambi and Giovanni Chimienti and Monica Montefalcone and Annalisa Azzola and Torcuato Pulido Mantas and Simonetta Fraschetti and Giulia Ceccherelli and Silvija Kipson and Tatjana Bakran‐Petricioli and Donat Petricioli and Carlos Jimenez and Stelios Katsanevakis and Inci Tuney Kizilkaya and Zafer Kizilkaya and Stephane Sartoretto and Rouanet Elodie and Sandrine Ruitton and Steeve Comeau and Jean‐Pierre Gattuso and Jean‐Georges Harmelin}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/09/2022-Garrabou-Global-Change-Biology-59.pdf https://onlinelibrary.wiley.com/doi/10.1111/gcb.16301}, doi = {10.1111/gcb.16301}, issn = {1354-1013, 1365-2486}, year = {2022}, date = {2022-10-01}, urldate = {2022-09-26}, journal = {Global Change Biology}, volume = {28}, number = {19}, pages = {5708--5725}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Katharios, Pantelis; Kokkari, Constantina; Cascarano, Maria Chiara; Gyparakis, Stylianos; Aggelidaki, Chrissi; Antonakaki, Ioanna; Vargiakaki, Efthimia A fish kill at the Aposelemis dam (Crete, Greece) caused by heavy parasitism by textitIchthyobodo sp. Journal Article Journal of Fish Diseases, pp. jfd.13717, 2022, ISSN: 0140-7775, 1365-2761. @article{katharios_fish_2022, title = {A fish kill at the Aposelemis dam (Crete, Greece) caused by heavy parasitism by textitIchthyobodo sp.}, author = {Pantelis Katharios and Constantina Kokkari and Maria Chiara Cascarano and Stylianos Gyparakis and Chrissi Aggelidaki and Ioanna Antonakaki and Efthimia Vargiakaki}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/09/2022-Katharios-Journal-of-Fish-Diseases-2022-Katharios-A-fish-kill-at-the-Aposelemis-dam-Crete-Greece-caused-by-heavy-parasitism-63.pdf https://onlinelibrary.wiley.com/doi/10.1111/jfd.13717}, doi = {10.1111/jfd.13717}, issn = {0140-7775, 1365-2761}, year = {2022}, date = {2022-09-01}, urldate = {2022-09-26}, journal = {Journal of Fish Diseases}, pages = {jfd.13717}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Georgopoulou, Dimitra G; Fanouraki, Eleftheria; Voskakis, Dimitris; Mitrizakis, Nikos; Papandroulakis, Nikos European seabass show variable responses in their group swimming features after tag implantation Journal Article Frontiers in Animal Science, 3 , pp. 997948, 2022, ISSN: 2673-6225. @article{georgopoulou_european_2022, title = {European seabass show variable responses in their group swimming features after tag implantation}, author = {Dimitra G Georgopoulou and Eleftheria Fanouraki and Dimitris Voskakis and Nikos Mitrizakis and Nikos Papandroulakis}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/09/2022-Georgopoulou-62.pdf https://www.frontiersin.org/articles/10.3389/fanim.2022.997948/full}, doi = {10.3389/fanim.2022.997948}, issn = {2673-6225}, year = {2022}, date = {2022-09-01}, urldate = {2022-09-26}, journal = {Frontiers in Animal Science}, volume = {3}, pages = {997948}, abstract = {The usefulness of acoustic telemetry on the study of movements, interactions, and behaviors has been revealed by many field and laboratory studies. The process of attaching acoustic tags on fish can, however, impact their physiological, behavioral, and growth performance traits. The potential negative effects are still unknown for several species and behavioral attributes. Previous studies have attempted to shed light on the effects of tag implantation on fish, focusing mainly on fish growth and physiological parameters, and one or two behavioral properties mainly on the individual level. However, the effect of this procedure could also be expressed at the group level. This study investigated the short-term effects of dummy and active body-implanted acoustic tags on the group-level swimming performance of adult European seabass ( Dicentrarchus labrax ) using optical flow analysis. We studied four main swimming performance properties—group speed, alignment (polarization), cohesion, and exploratory behavior. To help in the interpretation of any detected differences, physiological stress-related parameters were also extracted. The results show that the tag implantation procedure has variable effects on the different swimming performance attributes of fish. Group cohesion, polarization, and the group’s exploratory tendency were significantly impacted initially, and the effect persisted but to a lesser extent two weeks after surgery. In contrast, group speed was not affected initially but showed a significant decrease in comparison with the control group two weeks post-surgery. In addition, the physiological parameters tested did not show any significant difference between the control and the treated group 14 days after the onset of the experiment. The findings suggest that the effect of tagging is non-trivial, leading to responses and response times that could affect behavioral studies carried out using acoustic telemetry.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The usefulness of acoustic telemetry on the study of movements, interactions, and behaviors has been revealed by many field and laboratory studies. The process of attaching acoustic tags on fish can, however, impact their physiological, behavioral, and growth performance traits. The potential negative effects are still unknown for several species and behavioral attributes. Previous studies have attempted to shed light on the effects of tag implantation on fish, focusing mainly on fish growth and physiological parameters, and one or two behavioral properties mainly on the individual level. However, the effect of this procedure could also be expressed at the group level. This study investigated the short-term effects of dummy and active body-implanted acoustic tags on the group-level swimming performance of adult European seabass ( Dicentrarchus labrax ) using optical flow analysis. We studied four main swimming performance properties—group speed, alignment (polarization), cohesion, and exploratory behavior. To help in the interpretation of any detected differences, physiological stress-related parameters were also extracted. The results show that the tag implantation procedure has variable effects on the different swimming performance attributes of fish. Group cohesion, polarization, and the group’s exploratory tendency were significantly impacted initially, and the effect persisted but to a lesser extent two weeks after surgery. In contrast, group speed was not affected initially but showed a significant decrease in comparison with the control group two weeks post-surgery. In addition, the physiological parameters tested did not show any significant difference between the control and the treated group 14 days after the onset of the experiment. The findings suggest that the effect of tagging is non-trivial, leading to responses and response times that could affect behavioral studies carried out using acoustic telemetry. |
Foundation, Tara Ocean; Abreu, Andre; Bourgois, Etienne; Gristwood, Adam; Troublé, Romain; Oceans, Tara; Acinas, Silvia G; Bork, Peer; Boss, Emmanuel; Bowler, Chris; Budinich, Marko; Chaffron, Samuel; de Vargas, Colomban; Delmont, Tom O; Eveillard, Damien; Guidi, Lionel; Iudicone, Daniele; Kandels, Stephanie; Morlon, Hélène; Lombard, Fabien; Pepperkok, Rainer; Karlusich, Juan José Pierella; Piganeau, Gwenael; Régimbeau, Antoine; Sommeria-Klein, Guilhem; Stemmann, Lars; Sullivan, Matthew B; Sunagawa, Shinichi; Wincker, Patrick; Zablocki, Olivier; (EMBL), European Molecular Biology Laboratory; Arendt, Detlev; Bilic, Josipa; Finn, Robert; Heard, Edith; Rouse, Brendan; Vamathevan, Jessica; (EMBRC-ERIC), European Marine Biological Resource Centre European Research Infrastructure Consortium -; Casotti, Raffaella; Cancio, Ibon; Cunliffe, Michael; Kervella, Anne Emmanuelle; Kooistra, Wiebe H C F; Obst, Matthias; Pade, Nicolas; Power, Deborah M; Santi, Ioulia; Tsagaraki, Tatiana Margo; Vanaverbeke, Jan Priorities for ocean microbiome research Journal Article Nature Microbiology, 7 (7), pp. 937–947, 2022, ISSN: 2058-5276. @article{tara_ocean_foundation_priorities_2022, title = {Priorities for ocean microbiome research}, author = {Tara Ocean Foundation and Andre Abreu and Etienne Bourgois and Adam Gristwood and Romain Troublé and Tara Oceans and Silvia G Acinas and Peer Bork and Emmanuel Boss and Chris Bowler and Marko Budinich and Samuel Chaffron and Colomban de Vargas and Tom O Delmont and Damien Eveillard and Lionel Guidi and Daniele Iudicone and Stephanie Kandels and Hélène Morlon and Fabien Lombard and Rainer Pepperkok and Juan José Pierella Karlusich and Gwenael Piganeau and Antoine Régimbeau and Guilhem Sommeria-Klein and Lars Stemmann and Matthew B Sullivan and Shinichi Sunagawa and Patrick Wincker and Olivier Zablocki and European Molecular Biology Laboratory (EMBL) and Detlev Arendt and Josipa Bilic and Robert Finn and Edith Heard and Brendan Rouse and Jessica Vamathevan and European Marine Biological Resource Centre - European Research Infrastructure Consortium (EMBRC-ERIC) and Raffaella Casotti and Ibon Cancio and Michael Cunliffe and Anne Emmanuelle Kervella and Wiebe H C F Kooistra and Matthias Obst and Nicolas Pade and Deborah M Power and Ioulia Santi and Tatiana Margo Tsagaraki and Jan Vanaverbeke}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/07/2022-Sandi-et-al.-Priorities_for_ocean_microbiome_research-51.pdf https://www.nature.com/articles/s41564-022-01145-5}, doi = {10.1038/s41564-022-01145-5}, issn = {2058-5276}, year = {2022}, date = {2022-07-01}, urldate = {2022-07-29}, journal = {Nature Microbiology}, volume = {7}, number = {7}, pages = {937--947}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Sarropoulou, Xenia; Tsaparis, Dimitris; Tsagarakis, Konstantinos; Badouvas, Nicholas; Tsigenopoulos, Costas S Different patterns of population structure and genetic diversity of three mesopelagic fishes in the Greek Seas Journal Article Mediterranean Marine Science, 23 (3), pp. 536–545, 2022, ISSN: 1791-6763. @article{sarropoulou_different_2022, title = {Different patterns of population structure and genetic diversity of three mesopelagic fishes in the Greek Seas}, author = {Xenia Sarropoulou and Dimitris Tsaparis and Konstantinos Tsagarakis and Nicholas Badouvas and Costas S Tsigenopoulos}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/07/2022-Sarropoulou-MMS-49.pdf https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/28567}, doi = {10.12681/mms.28567}, issn = {1791-6763}, year = {2022}, date = {2022-06-01}, urldate = {2022-07-29}, journal = {Mediterranean Marine Science}, volume = {23}, number = {3}, pages = {536--545}, abstract = {Mesopelagic fishes are among the most abundant groups of vertebrates on Earth. Despite their unique biological and ecological traits, research in this group has been particularly scarce. The present study investigates the intraspecific genetic diversity of three mesopelagic fishes (Hygophum benoiti, Maurolicus muelleri, and Benthosema glaciale) in the Greek Seas. Analyses of three mitochondrial DNA genes (COI, 12S, and 16S) from a total of 168 samples revealed a lack of genetic structure for M. muelleri and B. glaciale across the studied area. However, H. benoiti specimens from the Corinthian Gulf were differentiated from the rest of the populations, suggesting that the limited connection between the Corinthian and neighboring seas may act as a barrier to gene flow. Furthermore, the COI data of this study were co-analyzed with publicly available sequences, demonstrating lack of phylogeographic structure for all three species through their distribution range. Therefore, even though indications of genetic differentiation were observed, the three mesopelagic fishes are generally characterized by genetic homogeneity, which may be the result of their recent evolutionary history.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Mesopelagic fishes are among the most abundant groups of vertebrates on Earth. Despite their unique biological and ecological traits, research in this group has been particularly scarce. The present study investigates the intraspecific genetic diversity of three mesopelagic fishes (Hygophum benoiti, Maurolicus muelleri, and Benthosema glaciale) in the Greek Seas. Analyses of three mitochondrial DNA genes (COI, 12S, and 16S) from a total of 168 samples revealed a lack of genetic structure for M. muelleri and B. glaciale across the studied area. However, H. benoiti specimens from the Corinthian Gulf were differentiated from the rest of the populations, suggesting that the limited connection between the Corinthian and neighboring seas may act as a barrier to gene flow. Furthermore, the COI data of this study were co-analyzed with publicly available sequences, demonstrating lack of phylogeographic structure for all three species through their distribution range. Therefore, even though indications of genetic differentiation were observed, the three mesopelagic fishes are generally characterized by genetic homogeneity, which may be the result of their recent evolutionary history. |
Villanueva, Beatriz; Fernández, Almudena; Peiró-Pastor, Ramón; Peñaloza, Carolina; Houston, Ross D; Sonesson, Anna K; Tsigenopoulos, Costas S; Bargelloni, Luca; Gamsız, Kutsal; Karahan, Bilge; Gökçek, Emel Ö; Fernández, Jesús; Saura, María Aquaculture Reports, 24 , pp. 101145, 2022, ISSN: 23525134. @article{villanueva_population_2022, title = {Population structure and genetic variability in wild and farmed Mediterranean populations of gilthead seabream and European seabass inferred from a 60K combined species SNP array}, author = {Beatriz Villanueva and Almudena Fernández and Ramón Peiró-Pastor and Carolina Peñaloza and Ross D Houston and Anna K Sonesson and Costas S Tsigenopoulos and Luca Bargelloni and Kutsal Gamsız and Bilge Karahan and Emel Ö Gökçek and Jesús Fernández and María Saura}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/07/2022-Villanueva-37.pdf https://linkinghub.elsevier.com/retrieve/pii/S2352513422001417}, doi = {10.1016/j.aqrep.2022.101145}, issn = {23525134}, year = {2022}, date = {2022-06-01}, urldate = {2022-07-29}, journal = {Aquaculture Reports}, volume = {24}, pages = {101145}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
de Mello, Paulo H; Divanach, Pascal; Papadakis, Ioannis E Aquaculture Research, 53 (9), pp. 3416–3429, 2022, ISSN: 1355-557X, 1365-2109. @article{de_mello_temperature_2022, title = {Temperature influences growth, digestive system ontogeny and lipids deposition in the liver in gilthead seabream ( textitSparus aurata ) larvae and juveniles}, author = {Paulo H de Mello and Pascal Divanach and Ioannis E Papadakis}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/07/2022-de-Mello-ARE-pre-print-44.pdf https://onlinelibrary.wiley.com/doi/10.1111/are.15849}, doi = {10.1111/are.15849}, issn = {1355-557X, 1365-2109}, year = {2022}, date = {2022-06-01}, urldate = {2022-07-29}, journal = {Aquaculture Research}, volume = {53}, number = {9}, pages = {3416--3429}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Skouradakis, Grigorios; Dounas, Costas; Androulakis, Dimitrios N; Papadaki, Maria; Koulouri, Panayota; Pavlidis, Michail A Study of Arca noae (Linnaeus, 1758) in Elounda Bay, Crete, Eastern Mediterranean Journal Article Journal of Marine Science and Engineering, 10 (5), pp. 673, 2022, ISSN: 2077-1312. @article{skouradakis_study_2022, title = {A Study of Arca noae (Linnaeus, 1758) in Elounda Bay, Crete, Eastern Mediterranean}, author = {Grigorios Skouradakis and Costas Dounas and Dimitrios N Androulakis and Maria Papadaki and Panayota Koulouri and Michail Pavlidis}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/07/2022-Skouradakis-jmse-38.pdf https://www.mdpi.com/2077-1312/10/5/673}, doi = {10.3390/jmse10050673}, issn = {2077-1312}, year = {2022}, date = {2022-05-01}, urldate = {2022-07-29}, journal = {Journal of Marine Science and Engineering}, volume = {10}, number = {5}, pages = {673}, abstract = {There is growing interest about marine bivalve aquaculture globally, not only for the market value of the goods produced, but also for the socio-economic and environmental services that this activity can provide. Arca noae is an endemic Mediterranean bivalve of commercial value, whose previously undescribed population in Elounda Bay we studied in terms of its structure and reproduction, while constructing a timeseries of the basic environmental parameters of the bay, thus, gaining fundamental knowledge for the potential future exploitation of the species in the area. We found a variable spatial distribution of arks in the study area, with local high peaks in the population density, consisting of smaller size individuals, in comparison to other areas. Because of protandry of the species, human pressure on this population could have a strong negative effect, by targeting the limited numbers of large females in the study area. The reproduction pattern was similar to the reports from other Mediterranean locations. The abiotic conditions in Elounda Bay differed from those in the adjacent coastal zone, confirming that the Bay is a unique semi-enclosed marine area in the island of Crete.}, keywords = {}, pubstate = {published}, tppubtype = {article} } There is growing interest about marine bivalve aquaculture globally, not only for the market value of the goods produced, but also for the socio-economic and environmental services that this activity can provide. Arca noae is an endemic Mediterranean bivalve of commercial value, whose previously undescribed population in Elounda Bay we studied in terms of its structure and reproduction, while constructing a timeseries of the basic environmental parameters of the bay, thus, gaining fundamental knowledge for the potential future exploitation of the species in the area. We found a variable spatial distribution of arks in the study area, with local high peaks in the population density, consisting of smaller size individuals, in comparison to other areas. Because of protandry of the species, human pressure on this population could have a strong negative effect, by targeting the limited numbers of large females in the study area. The reproduction pattern was similar to the reports from other Mediterranean locations. The abiotic conditions in Elounda Bay differed from those in the adjacent coastal zone, confirming that the Bay is a unique semi-enclosed marine area in the island of Crete. |
Digenis, Markos; Arvanitidis, Christos; Dailianis, Thanos; Gerovasileiou, Vasilis Comparative Study of Marine Cave Communities in a Protected Area of the South-Eastern Aegean Sea, Greece Journal Article Journal of Marine Science and Engineering, 10 (5), pp. 660, 2022, ISSN: 2077-1312. @article{digenis_comparative_2022, title = {Comparative Study of Marine Cave Communities in a Protected Area of the South-Eastern Aegean Sea, Greece}, author = {Markos Digenis and Christos Arvanitidis and Thanos Dailianis and Vasilis Gerovasileiou}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/07/2022-Digenis-jmse-40.pdf https://www.mdpi.com/2077-1312/10/5/660}, doi = {10.3390/jmse10050660}, issn = {2077-1312}, year = {2022}, date = {2022-05-01}, urldate = {2022-07-29}, journal = {Journal of Marine Science and Engineering}, volume = {10}, number = {5}, pages = {660}, abstract = {Although more than 600 marine caves have been recorded so far along the Greek coasts of the Aegean Sea (Eastern Mediterranean), only a few have been systematically studied for their biodiversity. In this study, the benthic communities of six marine caves within a Protected Area of South-Eastern Aegean were studied for the first time, both qualitatively and quantitatively. The association of different geomorphological and topographical factors of the caves with the benthic community structure was investigated. A total of 120 photographic quadrats covering the entrance and semi-dark cave zones were analysed, with regard to coverage and taxon abundance, while motile taxa were qualitatively recorded by visual census. The ecological quality status of the caves was also assessed under an ecosystem-based approach. In total, 81 sessile and 45 motile taxa were recorded, including 12 protected and 10 non-indigenous species. Multivariate community analysis demonstrated that the geomorphological and topographical variables of the caves are significantly associated with the observed biotic patterns. The ecological quality of the caves was assessed as poor or moderate according to the CavEBQI index, highlighting the necessity for systematic monitoring. This study paves the way for similar studies in marine cave habitats aiming at the development of management and conservation actions.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Although more than 600 marine caves have been recorded so far along the Greek coasts of the Aegean Sea (Eastern Mediterranean), only a few have been systematically studied for their biodiversity. In this study, the benthic communities of six marine caves within a Protected Area of South-Eastern Aegean were studied for the first time, both qualitatively and quantitatively. The association of different geomorphological and topographical factors of the caves with the benthic community structure was investigated. A total of 120 photographic quadrats covering the entrance and semi-dark cave zones were analysed, with regard to coverage and taxon abundance, while motile taxa were qualitatively recorded by visual census. The ecological quality status of the caves was also assessed under an ecosystem-based approach. In total, 81 sessile and 45 motile taxa were recorded, including 12 protected and 10 non-indigenous species. Multivariate community analysis demonstrated that the geomorphological and topographical variables of the caves are significantly associated with the observed biotic patterns. The ecological quality of the caves was assessed as poor or moderate according to the CavEBQI index, highlighting the necessity for systematic monitoring. This study paves the way for similar studies in marine cave habitats aiming at the development of management and conservation actions. |
Intze, Evangelia; Lagkouvardos, Ilias DivCom: A Tool for Systematic Partition of Groups of Microbial Profiles Into Intrinsic Subclusters and Distance-Based Subgroup Comparisons Journal Article Frontiers in Bioinformatics, 2 , pp. 864382, 2022, ISSN: 2673-7647. @article{intze_divcom_2022, title = {DivCom: A Tool for Systematic Partition of Groups of Microbial Profiles Into Intrinsic Subclusters and Distance-Based Subgroup Comparisons}, author = {Evangelia Intze and Ilias Lagkouvardos}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/07/2022-Intze-DivCom-42.pdf https://www.frontiersin.org/articles/10.3389/fbinf.2022.864382/full}, doi = {10.3389/fbinf.2022.864382}, issn = {2673-7647}, year = {2022}, date = {2022-05-01}, urldate = {2022-07-29}, journal = {Frontiers in Bioinformatics}, volume = {2}, pages = {864382}, abstract = {When analyzing microbiome data, one of the main objectives is to effectively compare the microbial profiles of samples belonging to different groups. Beta diversity measures the level of similarity among samples, usually in the form of dissimilarity matrices. The use of suitable statistical tests in conjunction with those matrices typically provides us with all the necessary information to evaluate the overall similarity of groups of microbial communities. However, in some cases, this approach can lead us to deceptive conclusions, mainly due to the uneven dispersions of the groups and the existence of unique or unexpected substructures in the dataset. To address these issues, we developed divide and compare (DivCom), an automated tool for advanced beta diversity analysis. DivCom reveals the inner structure of groups by dividing their samples into the appropriate number of clusters and then compares the distances of every profile to the centers of these clusters. This information can be used for determining the existing interrelation of the groups. The proposed methodology and the developed tool were assessed by comparing the response of anemic patients with or without inflammatory bowel disease to different iron replacement therapies. DivCom generated results that revealed the inner structure of the dataset, evaluated the relationship among the clusters, and assessed the effect of the treatments. The DivCom tool is freely available at: https://github.com/Lagkouvardos/DivCom .}, keywords = {}, pubstate = {published}, tppubtype = {article} } When analyzing microbiome data, one of the main objectives is to effectively compare the microbial profiles of samples belonging to different groups. Beta diversity measures the level of similarity among samples, usually in the form of dissimilarity matrices. The use of suitable statistical tests in conjunction with those matrices typically provides us with all the necessary information to evaluate the overall similarity of groups of microbial communities. However, in some cases, this approach can lead us to deceptive conclusions, mainly due to the uneven dispersions of the groups and the existence of unique or unexpected substructures in the dataset. To address these issues, we developed divide and compare (DivCom), an automated tool for advanced beta diversity analysis. DivCom reveals the inner structure of groups by dividing their samples into the appropriate number of clusters and then compares the distances of every profile to the centers of these clusters. This information can be used for determining the existing interrelation of the groups. The proposed methodology and the developed tool were assessed by comparing the response of anemic patients with or without inflammatory bowel disease to different iron replacement therapies. DivCom generated results that revealed the inner structure of the dataset, evaluated the relationship among the clusters, and assessed the effect of the treatments. The DivCom tool is freely available at: https://github.com/Lagkouvardos/DivCom . |
Rallis, Ioannis; Chatzigeorgiou, Giorgos; Florido, Marta; Sedano, Francisco; Procopiou, Avgi; Chertz-Bynichaki, Melina; Vernadou, Emmanouela; Plaiti, Wanda; Koulouri, Panayota; Dounas, Costas; Gerovasileiou, Vasilis; Dailianis, Thanos Early Succession Patterns of Benthic Assemblages on Artificial Reefs in the Oligotrophic Eastern Mediterranean Basin Journal Article Journal of Marine Science and Engineering, 10 (5), pp. 620, 2022, ISSN: 2077-1312. @article{rallis_early_2022, title = {Early Succession Patterns of Benthic Assemblages on Artificial Reefs in the Oligotrophic Eastern Mediterranean Basin}, author = {Ioannis Rallis and Giorgos Chatzigeorgiou and Marta Florido and Francisco Sedano and Avgi Procopiou and Melina Chertz-Bynichaki and Emmanouela Vernadou and Wanda Plaiti and Panayota Koulouri and Costas Dounas and Vasilis Gerovasileiou and Thanos Dailianis}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/05/2022-Rallis-jmse-35.pdf https://www.mdpi.com/2077-1312/10/5/620}, doi = {10.3390/jmse10050620}, issn = {2077-1312}, year = {2022}, date = {2022-05-01}, urldate = {2022-05-09}, journal = {Journal of Marine Science and Engineering}, volume = {10}, number = {5}, pages = {620}, abstract = {The colonization of artificial structures by benthic organisms in the marine realm is known to be affected by the general trophic patterns of the biogeographical zone and the prevailing environmental traits at the local scale. The present work aims to present quantitative data on the early settlement progress of macrofaunal benthic assemblages developing on artificial reefs (ARs) deployed at the Underwater Biotechnological Park of Crete (UBPC) in the oligotrophic Eastern Mediterranean. Visual census and subsequent image analysis combined with scraped quadrats were used to describe the establishment of the communities and their development over three consecutive campaigns, spanning 5 years post-deployment. Macroalgae consistently dominated in terms of coverage, while sessile invertebrates displayed different patterns over the years. Polychaeta and Bryozoa were gradually replaced by Cnidaria, while Porifera and Mollusca displayed an increasing trend over the years. Motile benthos was mainly represented by Mollusca, while the abundance of Polychaeta increased in contrast to that of Crustacea. For both sessile and motile assemblages, significant differences were observed among the years. The results of this study indicate that ecological succession is still ongoing, and further improvement in the monitoring methodology can assist towards a more accurate assessment of the community composition in complex AR structures.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The colonization of artificial structures by benthic organisms in the marine realm is known to be affected by the general trophic patterns of the biogeographical zone and the prevailing environmental traits at the local scale. The present work aims to present quantitative data on the early settlement progress of macrofaunal benthic assemblages developing on artificial reefs (ARs) deployed at the Underwater Biotechnological Park of Crete (UBPC) in the oligotrophic Eastern Mediterranean. Visual census and subsequent image analysis combined with scraped quadrats were used to describe the establishment of the communities and their development over three consecutive campaigns, spanning 5 years post-deployment. Macroalgae consistently dominated in terms of coverage, while sessile invertebrates displayed different patterns over the years. Polychaeta and Bryozoa were gradually replaced by Cnidaria, while Porifera and Mollusca displayed an increasing trend over the years. Motile benthos was mainly represented by Mollusca, while the abundance of Polychaeta increased in contrast to that of Crustacea. For both sessile and motile assemblages, significant differences were observed among the years. The results of this study indicate that ecological succession is still ongoing, and further improvement in the monitoring methodology can assist towards a more accurate assessment of the community composition in complex AR structures. |
Droubogiannis, Stavros; Katharios, Pantelis Pathogens, 11 (6), pp. 630, 2022, ISSN: 2076-0817. @article{droubogiannis_genomic_2022, title = {Genomic and Biological Profile of a Novel Bacteriophage, Vibrio phage Virtus, Which Improves Survival of Sparus aurata Larvae Challenged with Vibrio harveyi}, author = {Stavros Droubogiannis and Pantelis Katharios}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/07/2022-Droubogiannis-Pathogens-46.pdf https://www.mdpi.com/2076-0817/11/6/630}, doi = {10.3390/pathogens11060630}, issn = {2076-0817}, year = {2022}, date = {2022-05-01}, urldate = {2022-07-29}, journal = {Pathogens}, volume = {11}, number = {6}, pages = {630}, abstract = {Due to the emergence of multidrug-resistant bacteria, commonly known as “superbugs”, phage therapy for the control of bacterial diseases rose in popularity. In this context, the use of phages for the management of many important bacterial diseases in the aquaculture environment is auspicious. Vibrio harveyi, a well-known and serious bacterial pathogen, is responsible for many disease outbreaks in aquaculture, resulting in huge economic and production losses. We isolated and fully characterized a novel bacteriophage, Vibrio phage Virtus, infecting V. harveyi strain VH2. Vibrio phage Virtus can infect a wide spectrum of Vibrio spp., including strains of V. harveyi, V. owensii, V. campbellii, V. parahaemolyticus, and V. mediterranei. It has a latent period of 40 min with an unusually high burst size of 3200 PFU/cell. Vibrio phage Virtus has a double-stranded DNA of 82,960 base pairs with 127 predicted open reading frames (ORFs). No virulence, antibiotic resistance, or integrase-encoding genes were detected. In vivo phage therapy trials in gilthead seabream, Sparus aurata, larvae demonstrated that Vibrio phage Virtus was able to significantly improve the survival of larvae for five days at a multiplicity of infection (MOI) of 10, which suggests that it can be an excellent candidate for phage therapy.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Due to the emergence of multidrug-resistant bacteria, commonly known as “superbugs”, phage therapy for the control of bacterial diseases rose in popularity. In this context, the use of phages for the management of many important bacterial diseases in the aquaculture environment is auspicious. Vibrio harveyi, a well-known and serious bacterial pathogen, is responsible for many disease outbreaks in aquaculture, resulting in huge economic and production losses. We isolated and fully characterized a novel bacteriophage, Vibrio phage Virtus, infecting V. harveyi strain VH2. Vibrio phage Virtus can infect a wide spectrum of Vibrio spp., including strains of V. harveyi, V. owensii, V. campbellii, V. parahaemolyticus, and V. mediterranei. It has a latent period of 40 min with an unusually high burst size of 3200 PFU/cell. Vibrio phage Virtus has a double-stranded DNA of 82,960 base pairs with 127 predicted open reading frames (ORFs). No virulence, antibiotic resistance, or integrase-encoding genes were detected. In vivo phage therapy trials in gilthead seabream, Sparus aurata, larvae demonstrated that Vibrio phage Virtus was able to significantly improve the survival of larvae for five days at a multiplicity of infection (MOI) of 10, which suggests that it can be an excellent candidate for phage therapy. |
Papadogiannis, Vasileios; Pennati, Alessandro; Parker, Hugo J; Rothbächer, Ute; Patthey, Cedric; Bronner, Marianne E; Shimeld, Sebastian M Hmx gene conservation identifies the origin of vertebrate cranial ganglia Journal Article Nature, 605 (7911), pp. 701–705, 2022, ISSN: 0028-0836, 1476-4687. @article{papadogiannis_hmx_2022, title = {Hmx gene conservation identifies the origin of vertebrate cranial ganglia}, author = {Vasileios Papadogiannis and Alessandro Pennati and Hugo J Parker and Ute Rothbächer and Cedric Patthey and Marianne E Bronner and Sebastian M Shimeld}, url = {https://www.nature.com/articles/s41586-022-04742-w.epdf?sharing_token=0xZJF5h0PJfpVUdefAf4R9RgN0jAjWel9jnR3ZoTv0PSCAbGc3qtbGpsTuLrcmb7ttAtuhgvjAvX1_k8Ag7g7vn007aa4q9FN_-aaVkwteXRU-W2Z6yGfxXTk4K9POn9sdB02QtTVBOCYrkTIU4SKKE5Vu5NqJLHbbWXMbZGPEI%3D https://www.nature.com/articles/s41586-022-04742-w}, doi = {10.1038/s41586-022-04742-w}, issn = {0028-0836, 1476-4687}, year = {2022}, date = {2022-05-01}, urldate = {2022-07-29}, journal = {Nature}, volume = {605}, number = {7911}, pages = {701--705}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Plis, Kamila; Niedziałkowska, Magdalena; Borowik, Tomasz; Lang, Johannes; Heddergott, Mike; Tiainen, Juha; Bunevich, Aleksey; Šprem, Nikica; Paule, Ladislav; Danilkin, Aleksey; Kholodova, Marina; Zvychaynaya, Elena; Kashinina, Nadezhda; Pokorny, Boštjan; Flajšman, Katarina; Paulauskas, Algimantas; Djan, Mihajla; Ristić, Zoran; Novák, Luboš; Kusza, Szilvia; Miller, Christine; Tsaparis, Dimitris; Stoyanov, Stoyan; Shkvyria, Maryna; Suchentrunk, Franz; Kutal, Miroslav; Lavadinović, Vukan; Šnjegota, Dragana; Krapal, Ana‐Maria; Dănilă, Gabriel; Veeroja, Rauno; Dulko, Elżbieta; Jędrzejewska, Bogumiła Pan‐European phylogeography of the European roe deer ( textitCapreolus capreolus ) Journal Article Ecology and Evolution, 12 (5), 2022, ISSN: 2045-7758, 2045-7758. @article{plis_paneuropean_2022, title = {Pan‐European phylogeography of the European roe deer ( textitCapreolus capreolus )}, author = {Kamila Plis and Magdalena Niedziałkowska and Tomasz Borowik and Johannes Lang and Mike Heddergott and Juha Tiainen and Aleksey Bunevich and Nikica Šprem and Ladislav Paule and Aleksey Danilkin and Marina Kholodova and Elena Zvychaynaya and Nadezhda Kashinina and Boštjan Pokorny and Katarina Flajšman and Algimantas Paulauskas and Mihajla Djan and Zoran Ristić and Luboš Novák and Szilvia Kusza and Christine Miller and Dimitris Tsaparis and Stoyan Stoyanov and Maryna Shkvyria and Franz Suchentrunk and Miroslav Kutal and Vukan Lavadinović and Dragana Šnjegota and Ana‐Maria Krapal and Gabriel Dănilă and Rauno Veeroja and Elżbieta Dulko and Bogumiła Jędrzejewska}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/07/2022-Pils-Ecology-and-Evolution-43.pdf https://onlinelibrary.wiley.com/doi/10.1002/ece3.8931}, doi = {10.1002/ece3.8931}, issn = {2045-7758, 2045-7758}, year = {2022}, date = {2022-05-01}, urldate = {2022-07-29}, journal = {Ecology and Evolution}, volume = {12}, number = {5}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Mente, Eleni; Bousdras, Thomas; Feidantsis, Konstantinos; Panteli, Nikolas; Mastoraki, Maria; Kormas, Konstantinos Ar.; Chatzifotis, Stavros; Piccolo, Giovanni; Gasco, Laura; Gai, Francesco; Martin, Samuel A M; Antonopoulou, Efthimia Tenebrio molitor larvae meal inclusion affects hepatic proteome and apoptosis and/or autophagy of three farmed fish species Journal Article Scientific Reports, 12 (1), pp. 121, 2022, ISSN: 2045-2322. @article{mente_tenebrio_2022, title = {Tenebrio molitor larvae meal inclusion affects hepatic proteome and apoptosis and/or autophagy of three farmed fish species}, author = {Eleni Mente and Thomas Bousdras and Konstantinos Feidantsis and Nikolas Panteli and Maria Mastoraki and Konstantinos Ar. Kormas and Stavros Chatzifotis and Giovanni Piccolo and Laura Gasco and Francesco Gai and Samuel A M Martin and Efthimia Antonopoulou}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/05/2022-Mente-SciRep-23.pdf https://www.nature.com/articles/s41598-021-03306-8}, doi = {10.1038/s41598-021-03306-8}, issn = {2045-2322}, year = {2022}, date = {2022-04-29}, urldate = {2022-05-09}, journal = {Scientific Reports}, volume = {12}, number = {1}, pages = {121}, abstract = {Abstract Herein, the effect of dietary inclusion of insect ( Tenebrio molitor ) meal on hepatic pathways of apoptosis and autophagy in three farmed fish species, gilthead seabream ( Sparus aurata ), European seabass ( Dicentrarchus labrax ) and rainbow trout ( Oncorhynchus mykiss ), fed diets at 25%, 50% and 60% insect meal inclusion levels respectively, was investigated. Hepatic proteome was examined by liver protein profiles from the three fish species, obtained by two-dimensional gel electrophoresis. Although cellular stress was evident in the three teleost species following insect meal, inclusion by T. molitor , D. labrax and O. mykiss suppressed apoptosis through induction of hepatic autophagy, while in S. aurata both cellular procedures were activated. Protein abundance showed that a total of 30, 81 and 74 spots were altered significantly in seabream, European seabass and rainbow trout, respectively. Insect meal inclusion resulted in individual protein abundance changes, with less number of proteins altered in gilthead seabream compared to European seabass and rainbow trout. This is the first study demonstrating that insect meal in fish diets is causing changes in liver protein abundances. However, a species-specific response both in the above mentioned bioindicators, indicates the need to strategically manage fish meal replacement in fish diets per species.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Herein, the effect of dietary inclusion of insect ( Tenebrio molitor ) meal on hepatic pathways of apoptosis and autophagy in three farmed fish species, gilthead seabream ( Sparus aurata ), European seabass ( Dicentrarchus labrax ) and rainbow trout ( Oncorhynchus mykiss ), fed diets at 25%, 50% and 60% insect meal inclusion levels respectively, was investigated. Hepatic proteome was examined by liver protein profiles from the three fish species, obtained by two-dimensional gel electrophoresis. Although cellular stress was evident in the three teleost species following insect meal, inclusion by T. molitor , D. labrax and O. mykiss suppressed apoptosis through induction of hepatic autophagy, while in S. aurata both cellular procedures were activated. Protein abundance showed that a total of 30, 81 and 74 spots were altered significantly in seabream, European seabass and rainbow trout, respectively. Insect meal inclusion resulted in individual protein abundance changes, with less number of proteins altered in gilthead seabream compared to European seabass and rainbow trout. This is the first study demonstrating that insect meal in fish diets is causing changes in liver protein abundances. However, a species-specific response both in the above mentioned bioindicators, indicates the need to strategically manage fish meal replacement in fish diets per species. |
Smyrli, Maria; Anka, Ishrat Zahan; Koutsoni, Olga; Dotsika, Eleni; Kyriazis, Ioannis D; Pavlidis, Michail; Katharios, Pantelis Development of autogenous vaccines for farmed European seabass against Aeromonas veronii using zebrafish as a model for efficacy assessment Journal Article Fish & Shellfish Immunology, 123 , pp. 381–387, 2022, ISSN: 10504648. @article{smyrli_development_2022, title = {Development of autogenous vaccines for farmed European seabass against Aeromonas veronii using zebrafish as a model for efficacy assessment}, author = {Maria Smyrli and Ishrat Zahan Anka and Olga Koutsoni and Eleni Dotsika and Ioannis D Kyriazis and Michail Pavlidis and Pantelis Katharios}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/05/2022-Smyrli-FSImmune-24-pre-print.pdf https://linkinghub.elsevier.com/retrieve/pii/S1050464822001450}, doi = {10.1016/j.fsi.2022.03.019}, issn = {10504648}, year = {2022}, date = {2022-04-01}, urldate = {2022-05-09}, journal = {Fish & Shellfish Immunology}, volume = {123}, pages = {381--387}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Formenti Giulio; Secomandi, Simona; Studer Bruno; Theodoridis Spyros; Thines Marco; Urban Lara;Theissinger Kathrin; Manousaki Tereza; Wörheide Gert; Wurm Yannick; Zammit Gabrielle... The era of reference genomes in conservation genomics Journal Article Trends in Ecology & Evolution, 37 , pp. 197-202, 2022, ISSN: 01695347. @article{FormentiGiulio2022, title = {The era of reference genomes in conservation genomics}, author = {Formenti, Giulio; Secomandi, Simona; Studer, Bruno; Theodoridis, Spyros; Thines, Marco; Urban, Lara;Theissinger, Kathrin; Manousaki, Tereza; Wörheide, Gert; Wurm, Yannick; Zammit, Gabrielle...}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/03/2022-Formenti-Trends-in-EE-10-1.pdf}, doi = {10.1016/j.tree.2021.11.008}, issn = {01695347}, year = {2022}, date = {2022-03-05}, journal = {Trends in Ecology & Evolution}, volume = {37}, pages = {197-202}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Bouranta, Andrianna; Tudose, Ioan Valentin; Georgescu, Luciana; Karaiskou, Anna; Vrithias, Nikolaos Rafail; Viskadourakis, Zacharias; Kenanakis, George; Sfakaki, Efsevia; Mitrizakis, Nikolaos; Strakantounas, George; Papandroulakis, Nikolaos; Romanitan, Cosmin; Pachiu, Cristina; Tutunaru, Oana; Barbu-Tudoran, Lucian; Suchea, Mirela Petruta; Koudoumas, Emmanouel 3D Printed Metal Oxide-Polymer Composite Materials for Antifouling Applications Journal Article Nanomaterials, 12 (6), pp. 917, 2022, ISSN: 2079-4991. @article{bouranta_3d_2022, title = {3D Printed Metal Oxide-Polymer Composite Materials for Antifouling Applications}, author = {Andrianna Bouranta and Ioan Valentin Tudose and Luciana Georgescu and Anna Karaiskou and Nikolaos Rafail Vrithias and Zacharias Viskadourakis and George Kenanakis and Efsevia Sfakaki and Nikolaos Mitrizakis and George Strakantounas and Nikolaos Papandroulakis and Cosmin Romanitan and Cristina Pachiu and Oana Tutunaru and Lucian Barbu-Tudoran and Mirela Petruta Suchea and Emmanouel Koudoumas}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/05/2022-Bouranta-nanomaterials-19.pdf https://www.mdpi.com/2079-4991/12/6/917}, doi = {10.3390/nano12060917}, issn = {2079-4991}, year = {2022}, date = {2022-03-01}, urldate = {2022-05-09}, journal = {Nanomaterials}, volume = {12}, number = {6}, pages = {917}, abstract = {Current technology to prevent biofouling usually relies on the use of toxic, biocide-containing materials, which can become a serious threat to marine ecosystems, affecting both targeted and nontargeted organisms. Therefore, the development of broad-spectrum, less toxic antifouling materials is a challenge for researchers; such materials would be quite important in applications like aquaculture. In this respect, surface chemistry, physical properties, durability and attachment scheme can play a vital role in the performance of the materials. In this work, acrylonitrile butadiene styrene (ABS)/micro ZnO or nano ZnO composite lattices with different metal oxide contents were developed using 3D printing. Their antifouling behavior was examined with respect to aquaculture applications by monitoring growth on them of the diatoms Navicula sp. and the monocellular algae Chlorella sp. with image analysis techniques. As shown, the presence of metal oxides in the composite materials can bring about antifouling ability at particular concentrations. The present study showed promising results, but further improvements are needed.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Current technology to prevent biofouling usually relies on the use of toxic, biocide-containing materials, which can become a serious threat to marine ecosystems, affecting both targeted and nontargeted organisms. Therefore, the development of broad-spectrum, less toxic antifouling materials is a challenge for researchers; such materials would be quite important in applications like aquaculture. In this respect, surface chemistry, physical properties, durability and attachment scheme can play a vital role in the performance of the materials. In this work, acrylonitrile butadiene styrene (ABS)/micro ZnO or nano ZnO composite lattices with different metal oxide contents were developed using 3D printing. Their antifouling behavior was examined with respect to aquaculture applications by monitoring growth on them of the diatoms Navicula sp. and the monocellular algae Chlorella sp. with image analysis techniques. As shown, the presence of metal oxides in the composite materials can bring about antifouling ability at particular concentrations. The present study showed promising results, but further improvements are needed. |
Cascarano, Maria Chiara; Ruetten, Maja; Vaughan, Lloyd; Tsertou, Maria Ioanna; Georgopoulou, Dimitra; Keklikoglou, Kleoniki; Papandroulakis, Nikos; Katharios, Pantelis Epitheliocystis in Greater Amberjack: Evidence of a Novel Causative Agent, Pathology, Immune Response and Epidemiological Findings Journal Article Microorganisms, 10 (3), pp. 627, 2022, ISSN: 2076-2607. @article{cascarano_epitheliocystis_2022, title = {Epitheliocystis in Greater Amberjack: Evidence of a Novel Causative Agent, Pathology, Immune Response and Epidemiological Findings}, author = {Maria Chiara Cascarano and Maja Ruetten and Lloyd Vaughan and Maria Ioanna Tsertou and Dimitra Georgopoulou and Kleoniki Keklikoglou and Nikos Papandroulakis and Pantelis Katharios}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/05/2022-Cascarano-Microorganisms-20.pdf https://www.mdpi.com/2076-2607/10/3/627}, doi = {10.3390/microorganisms10030627}, issn = {2076-2607}, year = {2022}, date = {2022-03-01}, urldate = {2022-05-09}, journal = {Microorganisms}, volume = {10}, number = {3}, pages = {627}, abstract = {Epitheliocystis is a fish gill disease caused by a broad range of intracellular bacteria infecting freshwater and marine fish worldwide. Here we report the occurrence and progression of epitheliocystis in greater amberjack reared in Crete (Greece). The disease appears to be caused mainly by a novel Betaproteobacteria belonging to the Candidatus Ichthyocystis genus with a second agent genetically similar to Ca. Parilichlamydia carangidicola coinfecting the gills in some cases. After a first detection of the disease in 2017, we investigated epitheliocystis in the following year’s cohort of greater amberjack juveniles (cohort 2018) transferred from inland tanks to the same cage farm in the open sea where the first outbreak was detected. This cohort was monitored for over a year together with stocks of gilthead seabream and meagre co-farmed in the same area. Our observations showed that epitheliocystis could be detected in greater amberjack gills as early as a month following the transfer to sea cages, with ionocytes at the base of the gill lamellae being initially infected. Cyst formation appears to trigger a proliferative response, leading to the fusion of lamellae, impairment of gill functions and subsequently to mortality. Lesions are characterized by infiltration of immune cells, indicating activation of the innate immune response. At later stages of the outbreak, cysts were no longer found in ionocytes but were observed in mucocytes at the trailing edge of the filament. Whole cysts appeared finally to be expelled from infected mucocytes directly into the water, which might constitute a novel means of dispersion of the infectious agents. Molecular screening indicates that meagre is not affected by this disease and confirms the presence of previously described epitheliocystis agents, Ca. Ichthyocystis sparus, Ca. Ichthyocystis hellenicum and Ca. Similichlamydia spp., in gilthead seabream. Prevalence data show that the bacteria persist in both gilthead seabream and greater amberjack cohorts after first infection.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Epitheliocystis is a fish gill disease caused by a broad range of intracellular bacteria infecting freshwater and marine fish worldwide. Here we report the occurrence and progression of epitheliocystis in greater amberjack reared in Crete (Greece). The disease appears to be caused mainly by a novel Betaproteobacteria belonging to the Candidatus Ichthyocystis genus with a second agent genetically similar to Ca. Parilichlamydia carangidicola coinfecting the gills in some cases. After a first detection of the disease in 2017, we investigated epitheliocystis in the following year’s cohort of greater amberjack juveniles (cohort 2018) transferred from inland tanks to the same cage farm in the open sea where the first outbreak was detected. This cohort was monitored for over a year together with stocks of gilthead seabream and meagre co-farmed in the same area. Our observations showed that epitheliocystis could be detected in greater amberjack gills as early as a month following the transfer to sea cages, with ionocytes at the base of the gill lamellae being initially infected. Cyst formation appears to trigger a proliferative response, leading to the fusion of lamellae, impairment of gill functions and subsequently to mortality. Lesions are characterized by infiltration of immune cells, indicating activation of the innate immune response. At later stages of the outbreak, cysts were no longer found in ionocytes but were observed in mucocytes at the trailing edge of the filament. Whole cysts appeared finally to be expelled from infected mucocytes directly into the water, which might constitute a novel means of dispersion of the infectious agents. Molecular screening indicates that meagre is not affected by this disease and confirms the presence of previously described epitheliocystis agents, Ca. Ichthyocystis sparus, Ca. Ichthyocystis hellenicum and Ca. Similichlamydia spp., in gilthead seabream. Prevalence data show that the bacteria persist in both gilthead seabream and greater amberjack cohorts after first infection. |
Cascarano, Maria Chiara; Katharios, Pantelis Expansion of the Beta-Proteobacterial Genus Ca. Ichthyocystis: A Case Report of Epitheliocystis in the Pompano Trachinotus ovatus Journal Article Pathogens, 11 (4), pp. 421, 2022, ISSN: 2076-0817. @article{cascarano_expansion_2022, title = {Expansion of the Beta-Proteobacterial Genus Ca. Ichthyocystis: A Case Report of Epitheliocystis in the Pompano Trachinotus ovatus}, author = {Maria Chiara Cascarano and Pantelis Katharios}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/05/2022-Cascarano-Pathogens-28.pdf https://www.mdpi.com/2076-0817/11/4/421}, doi = {10.3390/pathogens11040421}, issn = {2076-0817}, year = {2022}, date = {2022-03-01}, urldate = {2022-05-09}, journal = {Pathogens}, volume = {11}, number = {4}, pages = {421}, abstract = {Epitheliocystis is a disease caused by a wide variety of host-specific intracellular bacteria infecting fish gills. In the Mediterranean Sea, epitheliocystis has been recently associated with a novel genus of beta-proteobacteria, the Ca. Ichthyocystis genus. In the present study, we report a case of epitheliocystis in a wild-caught specimen of pompano Trachinotus ovatus in Crete, Greece. Molecular analysis of partial 16s rRNA sequence led to the discovery of a putative novel species of the Ca. Ichthyocystis genus. Investigation of the phylogenetic relationship between closely related sequences deposited in NCBI suggests that bacterial ancestors in gilthead seabream might have a pivotal role in the differentiation of genus.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Epitheliocystis is a disease caused by a wide variety of host-specific intracellular bacteria infecting fish gills. In the Mediterranean Sea, epitheliocystis has been recently associated with a novel genus of beta-proteobacteria, the Ca. Ichthyocystis genus. In the present study, we report a case of epitheliocystis in a wild-caught specimen of pompano Trachinotus ovatus in Crete, Greece. Molecular analysis of partial 16s rRNA sequence led to the discovery of a putative novel species of the Ca. Ichthyocystis genus. Investigation of the phylogenetic relationship between closely related sequences deposited in NCBI suggests that bacterial ancestors in gilthead seabream might have a pivotal role in the differentiation of genus. |
Vela-Avitúa, Sergio; Thorland, Ingunn; Bakopoulos, Vasileios; Papanna, Kantham; Dimitroglou, Arkadios; Kottaras, Eleftherios; Leonidas, Papaharisis; Guinand, Bruno; Tsigenopoulos, Costas S; Aslam, Muhammad L Frontiers in Genetics, 13 , pp. 804584, 2022, ISSN: 1664-8021. @article{vela-avitua_genetic_2022, title = {Genetic Basis for Resistance Against Viral Nervous Necrosis: GWAS and Potential of Genomic Prediction Explored in Farmed European Sea Bass (Dicentrarchus labrax)}, author = {Sergio Vela-Avitúa and Ingunn Thorland and Vasileios Bakopoulos and Kantham Papanna and Arkadios Dimitroglou and Eleftherios Kottaras and Papaharisis Leonidas and Bruno Guinand and Costas S Tsigenopoulos and Muhammad L Aslam}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/05/2022-Vela-Avitua-Frontiers-in-Gen-26.pdf}, doi = {10.3389/fgene.2022.804584}, issn = {1664-8021}, year = {2022}, date = {2022-03-01}, urldate = {2022-05-09}, journal = {Frontiers in Genetics}, volume = {13}, pages = {804584}, abstract = {Viral nervous necrosis (VNN) is an infectious disease caused by the red-spotted grouper nervous necrosis virus (RGNNV) in European sea bass and is considered a serious concern for the aquaculture industry with fry and juveniles being highly susceptible. To understand the genetic basis for resistance against VNN, a survival phenotype through the challenge test against the RGNNV was recorded in populations from multiple year classes (YC2016 and YC2017). A total of 4,851 individuals from 181 families were tested, and a subset (n∼1,535) belonging to 122 families was genotyped using a ∼57K Affymetrix Axiom array. The survival against the RGNNV showed low to moderate heritability with observed scale estimates of 0.18 and 0.25 obtained using pedigree vs. genomic information, respectively. The genome-wide association analysis showed a strong signal of quantitative trait loci (QTL) at LG12 which explained ∼33% of the genetic variance. The QTL region contained multiple genes ( ITPK1 , PLK4 , HSPA4L , REEP1 , CHMP2 , MRPL35 , and SCUBE ) with HSPA4L and/or REEP1 genes being highly relevant with a likely effect on host response in managing disease-associated symptoms. The results on the accuracy of predicting breeding values presented 20–43% advantage in accuracy using genomic over pedigree-based information which varied across model types and applied validation schemes.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Viral nervous necrosis (VNN) is an infectious disease caused by the red-spotted grouper nervous necrosis virus (RGNNV) in European sea bass and is considered a serious concern for the aquaculture industry with fry and juveniles being highly susceptible. To understand the genetic basis for resistance against VNN, a survival phenotype through the challenge test against the RGNNV was recorded in populations from multiple year classes (YC2016 and YC2017). A total of 4,851 individuals from 181 families were tested, and a subset (n∼1,535) belonging to 122 families was genotyped using a ∼57K Affymetrix Axiom array. The survival against the RGNNV showed low to moderate heritability with observed scale estimates of 0.18 and 0.25 obtained using pedigree vs. genomic information, respectively. The genome-wide association analysis showed a strong signal of quantitative trait loci (QTL) at LG12 which explained ∼33% of the genetic variance. The QTL region contained multiple genes ( ITPK1 , PLK4 , HSPA4L , REEP1 , CHMP2 , MRPL35 , and SCUBE ) with HSPA4L and/or REEP1 genes being highly relevant with a likely effect on host response in managing disease-associated symptoms. The results on the accuracy of predicting breeding values presented 20–43% advantage in accuracy using genomic over pedigree-based information which varied across model types and applied validation schemes. |
Cheimonopoulou, Maria; Koulouri, Panayota; Previati, Monica; Realdon, Giulia; Mokos, Melita; Mogias, Athanasios Implementation of a new research tool for evaluating Mediterranean Sea Literacy (MSL) of high school students: A pilot study Journal Article Mediterranean Marine Science, 23 (2), pp. 302–309, 2022, ISSN: 1791-6763, 1108-393X. @article{cheimonopoulou_implementation_2022, title = {Implementation of a new research tool for evaluating Mediterranean Sea Literacy (MSL) of high school students: A pilot study}, author = {Maria Cheimonopoulou and Panayota Koulouri and Monica Previati and Giulia Realdon and Melita Mokos and Athanasios Mogias}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/05/2022-Cheimonopoulou-MedMarSci-32-1.pdf https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/29712}, doi = {10.12681/mms.29712}, issn = {1791-6763, 1108-393X}, year = {2022}, date = {2022-03-01}, urldate = {2022-05-09}, journal = {Mediterranean Marine Science}, volume = {23}, number = {2}, pages = {302--309}, abstract = {The Mediterranean Sea is recognized as a key component in the development, economy, and culture of European, North African, and Middle East countries. With respect to heterogeneity across the region in different sectors, Ocean Literacy, though still in its infancy, is nevertheless a requisite for a better understanding of the two-way interaction between the Sea and its people. In the present study, marine issues in relation to the content knowledge of 154 high school students from the Mediterranean region were investigated by using a structured questionnaire based on the recently published Mediterranean Sea Literacy guide. Data analysis involved descriptive statistics to portray frequencies and knowledge scores of the participants, and inferential statistics to assess the effects of grade level on students’ knowledge. The study which focused for the first time on the unique features of the Mediterranean marine ecosystems, found the level of content knowledge of the participants to be low to moderate. It is therefore of the utmost importance for the organizations and networks working on marine issues in the Mediterranean Sea to develop synergies and coordinate research programmes to broaden engagement with human societies in the region.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The Mediterranean Sea is recognized as a key component in the development, economy, and culture of European, North African, and Middle East countries. With respect to heterogeneity across the region in different sectors, Ocean Literacy, though still in its infancy, is nevertheless a requisite for a better understanding of the two-way interaction between the Sea and its people. In the present study, marine issues in relation to the content knowledge of 154 high school students from the Mediterranean region were investigated by using a structured questionnaire based on the recently published Mediterranean Sea Literacy guide. Data analysis involved descriptive statistics to portray frequencies and knowledge scores of the participants, and inferential statistics to assess the effects of grade level on students’ knowledge. The study which focused for the first time on the unique features of the Mediterranean marine ecosystems, found the level of content knowledge of the participants to be low to moderate. It is therefore of the utmost importance for the organizations and networks working on marine issues in the Mediterranean Sea to develop synergies and coordinate research programmes to broaden engagement with human societies in the region. |
Nomikou, Paraskevi; Polymenakou, Paraskevi N; Rizzo, Andrea Luca; Petersen, Sven; Hannington, Mark; Kilias, Stephanos Pantelis; Papanikolaou, Dimitris; Escartin, Javier; Karantzalos, Konstantinos; Mertzimekis, Theodoros J; Antoniou, Varvara; Krokos, Mel; Grammatikopoulos, Lazaros; Italiano, Francesco; Caruso, Cinzia Giuseppina; Lazzaro, Gianluca; Longo, Manfredi; Scappuzzo, Sergio Sciré; D’Alessandro, Walter; Grassa, Fausto; Bejelou, Konstantina; Lampridou, Danai; Katsigera, Anna; Dura, Anne SANTORY: SANTORini’s Seafloor Volcanic ObservatorY Journal Article Frontiers in Marine Science, 9 , pp. 796376, 2022, ISSN: 2296-7745. @article{nomikou_santory_2022, title = {SANTORY: SANTORini’s Seafloor Volcanic ObservatorY}, author = {Paraskevi Nomikou and Paraskevi N Polymenakou and Andrea Luca Rizzo and Sven Petersen and Mark Hannington and Stephanos Pantelis Kilias and Dimitris Papanikolaou and Javier Escartin and Konstantinos Karantzalos and Theodoros J Mertzimekis and Varvara Antoniou and Mel Krokos and Lazaros Grammatikopoulos and Francesco Italiano and Cinzia Giuseppina Caruso and Gianluca Lazzaro and Manfredi Longo and Sergio Sciré Scappuzzo and Walter D’Alessandro and Fausto Grassa and Konstantina Bejelou and Danai Lampridou and Anna Katsigera and Anne Dura}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/05/2022-Nomikou-FroMarSci-31.pdf https://www.frontiersin.org/articles/10.3389/fmars.2022.796376/full}, doi = {10.3389/fmars.2022.796376}, issn = {2296-7745}, year = {2022}, date = {2022-03-01}, urldate = {2022-05-09}, journal = {Frontiers in Marine Science}, volume = {9}, pages = {796376}, abstract = {Submarine hydrothermal systems along active volcanic ridges and arcs are highly dynamic, responding to both oceanographic (e.g., currents, tides) and deep-seated geological forcing (e.g., magma eruption, seismicity, hydrothermalism, and crustal deformation, etc.). In particular, volcanic and hydrothermal activity may also pose profoundly negative societal impacts (tsunamis, the release of climate-relevant gases and toxic metal(loid)s). These risks are particularly significant in shallow (<1000m) coastal environments, as demonstrated by the January 2022 submarine paroxysmal eruption by the Hunga Tonga-Hunga Ha’apai Volcano that destroyed part of the island, and the October 2011 submarine eruption of El Hierro (Canary Islands) that caused vigorous upwelling, floating lava bombs, and natural seawater acidification. Volcanic hazards may be posed by the Kolumbo submarine volcano, which is part of the subduction-related Hellenic Volcanic Arc at the intersection between the Eurasian and African tectonic plates. There, the Kolumbo submarine volcano, 7 km NE of Santorini and part of Santorini’s volcanic complex, hosts an active hydrothermal vent field (HVF) on its crater floor (textasciitilde500m b.s.l.), which degasses boiling CO 2 –dominated fluids at high temperatures (textasciitilde265°C) with a clear mantle signature. Kolumbo’s HVF hosts actively forming seafloor massive sulfide deposits with high contents of potentially toxic, volatile metal(loid)s (As, Sb, Pb, Ag, Hg, and Tl). The proximity to highly populated/tourist areas at Santorini poses significant risks. However, we have limited knowledge of the potential impacts of this type of magmatic and hydrothermal activity, including those from magmatic gases and seismicity. To better evaluate such risks the activity of the submarine system must be continuously monitored with multidisciplinary and high resolution instrumentation as part of an in-situ observatory supported by discrete sampling and measurements. This paper is a design study that describes a new long-term seafloor observatory that will be installed within the Kolumbo volcano, including cutting-edge and innovative marine-technology that integrates hyperspectral imaging, temperature sensors, a radiation spectrometer, fluid/gas samplers, and pressure gauges. These instruments will be integrated into a hazard monitoring platform aimed at identifying the precursors of potentially disastrous explosive volcanic eruptions, earthquakes, landslides of the hydrothermally weakened volcanic edifice and the release of potentially toxic elements into the water column.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Submarine hydrothermal systems along active volcanic ridges and arcs are highly dynamic, responding to both oceanographic (e.g., currents, tides) and deep-seated geological forcing (e.g., magma eruption, seismicity, hydrothermalism, and crustal deformation, etc.). In particular, volcanic and hydrothermal activity may also pose profoundly negative societal impacts (tsunamis, the release of climate-relevant gases and toxic metal(loid)s). These risks are particularly significant in shallow (<1000m) coastal environments, as demonstrated by the January 2022 submarine paroxysmal eruption by the Hunga Tonga-Hunga Ha’apai Volcano that destroyed part of the island, and the October 2011 submarine eruption of El Hierro (Canary Islands) that caused vigorous upwelling, floating lava bombs, and natural seawater acidification. Volcanic hazards may be posed by the Kolumbo submarine volcano, which is part of the subduction-related Hellenic Volcanic Arc at the intersection between the Eurasian and African tectonic plates. There, the Kolumbo submarine volcano, 7 km NE of Santorini and part of Santorini’s volcanic complex, hosts an active hydrothermal vent field (HVF) on its crater floor (textasciitilde500m b.s.l.), which degasses boiling CO 2 –dominated fluids at high temperatures (textasciitilde265°C) with a clear mantle signature. Kolumbo’s HVF hosts actively forming seafloor massive sulfide deposits with high contents of potentially toxic, volatile metal(loid)s (As, Sb, Pb, Ag, Hg, and Tl). The proximity to highly populated/tourist areas at Santorini poses significant risks. However, we have limited knowledge of the potential impacts of this type of magmatic and hydrothermal activity, including those from magmatic gases and seismicity. To better evaluate such risks the activity of the submarine system must be continuously monitored with multidisciplinary and high resolution instrumentation as part of an in-situ observatory supported by discrete sampling and measurements. This paper is a design study that describes a new long-term seafloor observatory that will be installed within the Kolumbo volcano, including cutting-edge and innovative marine-technology that integrates hyperspectral imaging, temperature sensors, a radiation spectrometer, fluid/gas samplers, and pressure gauges. These instruments will be integrated into a hazard monitoring platform aimed at identifying the precursors of potentially disastrous explosive volcanic eruptions, earthquakes, landslides of the hydrothermally weakened volcanic edifice and the release of potentially toxic elements into the water column. |
Publications
2023 |
Recent Advances in Mycotoxin Determination in Fish Feed Ingredients Journal Article Molecules, 28 (6), pp. 2519, 2023, ISSN: 1420-3049. |
Exploring microbial functional biodiversity at the protein family level—From metagenomic sequence reads to annotated protein clusters Journal Article Frontiers in Bioinformatics, 3 , pp. 1157956, 2023, ISSN: 2673-7647. |
Frontiers in Microbiology, 14 , pp. 1078669, 2023, ISSN: 1664-302X. |
Metabolic models of human gut microbiota: Advances and challenges Journal Article Cell Systems, 14 (2), pp. 109–121, 2023, ISSN: 24054712. |
International Journal of Molecular Sciences, 24 (4), pp. 3937, 2023, ISSN: 1422-0067. |
Foods, 12 (3), pp. 505, 2023, ISSN: 2304-8158. |
Sardines at a junction: Seascape genomics reveals ecological and oceanographic drivers of variation in the textlessspan style= Journal Article Molecular Ecology, pp. mec.16840, 2023, ISSN: 0962-1083, 1365-294X. |
Effective algorithmic operational framework for fish texture evaluation in industry: Achieving maturity Journal Article Aquaculture and Fisheries, 8 (4), pp. 422–430, 2023, ISSN: 2468550X. |
Investigation of systemic granulomatosis in cultured meagre, Argyrosomus regius, using clinical metagenomics Journal Article Aquaculture, 567 , pp. 739249, 2023, ISSN: 00448486. |
Frontiers in Marine Science, 9 , pp. 907269, 2023, ISSN: 2296-7745. |
Structure and metabolic potential of the prokaryotic communities from the hydrothermal system of Paleochori Bay, Milos, Greece Journal Article Frontiers in Microbiology, 13 , pp. 1060168, 2023, ISSN: 1664-302X. |
Chromosome genome assembly for the meagre, Argyrosomus regius, reveals species adaptations and sciaenid sex-related locus evolution Journal Article Frontiers in Genetics, 13 , pp. 1081760, 2023, ISSN: 1664-8021. |
Aquaculture, 563 , pp. 738918, 2023, ISSN: 00448486. |
Antimicrobial drugs in aquaculture: use and abuse Incollection Present Knowledge in Food Safety, pp. 142–161, Elsevier, 2023, ISBN: 9780128194706. |
Aquaculture, 562 , pp. 738830, 2023, ISSN: 00448486. |
2022 |
Fish Physiology and Biochemistry, 48 (6), pp. 1665–1684, 2022, ISSN: 0920-1742, 1573-5168. |
New records of rare species in the Mediterranean Sea (December 2022) Journal Article Mediterranean Marine Science, 23 (4), pp. 968–994, 2022, ISSN: 1791-6763, 1108-393X. |
Deciphering the community structure and the functional potential of a hypersaline marsh microbial mat community Journal Article FEMS Microbiology Ecology, 98 (12), pp. fiac141, 2022, ISSN: 1574-6941. |
Sustainability, 14 (22), pp. 15202, 2022, ISSN: 2071-1050. |
Dissolved oxygen estimation in aquaculture sites using remote sensing and machine learning Journal Article Remote Sensing Applications: Society and Environment, 28 , pp. 100865, 2022, ISSN: 23529385. |
Transcriptome analysis of flathead grey mullet (Mugil cephalus) ovarian development induced by recombinant gonadotropin hormones Journal Article Frontiers in Physiology, 13 , pp. 1033445, 2022, ISSN: 1664-042X. |
Autophagy activation can partially rescue proteasome dysfunction‐mediated cardiac toxicity Journal Article Aging Cell, 2022, ISSN: 1474-9718, 1474-9726. |
Long‐duration remote underwater videos reveal that grazing by fishes is highly variable through time and dominated by non‐indigenous species Journal Article Remote Sensing in Ecology and Conservation, pp. rse2.311, 2022, ISSN: 2056-3485, 2056-3485. |
A function-based typology for Earth’s ecosystems Journal Article Nature, 610 (7932), pp. 513–518, 2022, ISSN: 0028-0836, 1476-4687. |
Animal Feed Science and Technology, 284 , pp. 115194, 2022, ISSN: 03778401. |
Aquaculture Reports, 27 , pp. 101347, 2022, ISSN: 23525134. |
Aquaculture, 561 , pp. 738674, 2022, ISSN: 00448486. |
Marine heatwaves drive recurrent mass mortalities in the Mediterranean Sea Journal Article Global Change Biology, 28 (19), pp. 5708–5725, 2022, ISSN: 1354-1013, 1365-2486. |
A fish kill at the Aposelemis dam (Crete, Greece) caused by heavy parasitism by textitIchthyobodo sp. Journal Article Journal of Fish Diseases, pp. jfd.13717, 2022, ISSN: 0140-7775, 1365-2761. |
European seabass show variable responses in their group swimming features after tag implantation Journal Article Frontiers in Animal Science, 3 , pp. 997948, 2022, ISSN: 2673-6225. |
Priorities for ocean microbiome research Journal Article Nature Microbiology, 7 (7), pp. 937–947, 2022, ISSN: 2058-5276. |
Different patterns of population structure and genetic diversity of three mesopelagic fishes in the Greek Seas Journal Article Mediterranean Marine Science, 23 (3), pp. 536–545, 2022, ISSN: 1791-6763. |
Aquaculture Reports, 24 , pp. 101145, 2022, ISSN: 23525134. |
Aquaculture Research, 53 (9), pp. 3416–3429, 2022, ISSN: 1355-557X, 1365-2109. |
A Study of Arca noae (Linnaeus, 1758) in Elounda Bay, Crete, Eastern Mediterranean Journal Article Journal of Marine Science and Engineering, 10 (5), pp. 673, 2022, ISSN: 2077-1312. |
Comparative Study of Marine Cave Communities in a Protected Area of the South-Eastern Aegean Sea, Greece Journal Article Journal of Marine Science and Engineering, 10 (5), pp. 660, 2022, ISSN: 2077-1312. |
DivCom: A Tool for Systematic Partition of Groups of Microbial Profiles Into Intrinsic Subclusters and Distance-Based Subgroup Comparisons Journal Article Frontiers in Bioinformatics, 2 , pp. 864382, 2022, ISSN: 2673-7647. |
Early Succession Patterns of Benthic Assemblages on Artificial Reefs in the Oligotrophic Eastern Mediterranean Basin Journal Article Journal of Marine Science and Engineering, 10 (5), pp. 620, 2022, ISSN: 2077-1312. |
Pathogens, 11 (6), pp. 630, 2022, ISSN: 2076-0817. |
Hmx gene conservation identifies the origin of vertebrate cranial ganglia Journal Article Nature, 605 (7911), pp. 701–705, 2022, ISSN: 0028-0836, 1476-4687. |
Pan‐European phylogeography of the European roe deer ( textitCapreolus capreolus ) Journal Article Ecology and Evolution, 12 (5), 2022, ISSN: 2045-7758, 2045-7758. |
Tenebrio molitor larvae meal inclusion affects hepatic proteome and apoptosis and/or autophagy of three farmed fish species Journal Article Scientific Reports, 12 (1), pp. 121, 2022, ISSN: 2045-2322. |
Development of autogenous vaccines for farmed European seabass against Aeromonas veronii using zebrafish as a model for efficacy assessment Journal Article Fish & Shellfish Immunology, 123 , pp. 381–387, 2022, ISSN: 10504648. |
The era of reference genomes in conservation genomics Journal Article Trends in Ecology & Evolution, 37 , pp. 197-202, 2022, ISSN: 01695347. |
3D Printed Metal Oxide-Polymer Composite Materials for Antifouling Applications Journal Article Nanomaterials, 12 (6), pp. 917, 2022, ISSN: 2079-4991. |
Epitheliocystis in Greater Amberjack: Evidence of a Novel Causative Agent, Pathology, Immune Response and Epidemiological Findings Journal Article Microorganisms, 10 (3), pp. 627, 2022, ISSN: 2076-2607. |
Expansion of the Beta-Proteobacterial Genus Ca. Ichthyocystis: A Case Report of Epitheliocystis in the Pompano Trachinotus ovatus Journal Article Pathogens, 11 (4), pp. 421, 2022, ISSN: 2076-0817. |
Frontiers in Genetics, 13 , pp. 804584, 2022, ISSN: 1664-8021. |
Implementation of a new research tool for evaluating Mediterranean Sea Literacy (MSL) of high school students: A pilot study Journal Article Mediterranean Marine Science, 23 (2), pp. 302–309, 2022, ISSN: 1791-6763, 1108-393X. |
SANTORY: SANTORini’s Seafloor Volcanic ObservatorY Journal Article Frontiers in Marine Science, 9 , pp. 796376, 2022, ISSN: 2296-7745. |