2021 |
Karamanlidis, Alexandros A; Skrbinšek, Tomaž; Amato, George; Dendrinos, Panagiotis; Gaughran, Stephen; Kasapidis, Panagiotis; Kopatz, Alexander; Stronen, Astrid Vik Sci Rep, 11 (1), pp. 373, 2021, ISSN: 2045-2322. @article{karamanlidis_genetic_2021, title = {Genetic and demographic history define a conservation strategy for earth’s most endangered pinniped, the Mediterranean monk seal Monachus monachus}, author = {Alexandros A Karamanlidis and Tomaž Skrbinšek and George Amato and Panagiotis Dendrinos and Stephen Gaughran and Panagiotis Kasapidis and Alexander Kopatz and Astrid Vik Stronen}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/02/2021-Karamanlidis-SciRep-3.pdf http://www.nature.com/articles/s41598-020-79712-1}, doi = {10.1038/s41598-020-79712-1}, issn = {2045-2322}, year = {2021}, date = {2021-12-01}, urldate = {2021-02-02}, journal = {Sci Rep}, volume = {11}, number = {1}, pages = {373}, abstract = {Abstract The Mediterranean monk seal (Monachus monachus) is a flagship species for marine conservation, but important aspects of its life history remain unknown. Concerns over imminent extinction motivated a nuclear DNA study of the species in its largest continuous subpopulation in the eastern Mediterranean Sea. Despite recent evidence of partial subpopulation recovery, we demonstrate that there is no reason for complacency, as the species still shares several traits that are characteristic of a critically endangered species: Mediterranean monk seals in the eastern Mediterranean survive in three isolated and genetically depauperate population clusters, with small effective population sizes and high levels of inbreeding. Our results indicated male philopatry over short distances, which is unexpected for a polygynous mammal. Such a pattern may be explained by the species’ unique breeding behavior, in which males defend aquatic territories near breeding sites, while females are often forced to search for new pupping areas. Immediate action is necessary to reverse the downward spiral of population decline, inbreeding accumulation and loss of genetic diversity. We propose concrete conservation measures for the Mediterranean monk seal focusing on reducing anthropogenic threats, increasing the population size and genetic diversity, and thus improving the long-term prospects of survival.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract The Mediterranean monk seal (Monachus monachus) is a flagship species for marine conservation, but important aspects of its life history remain unknown. Concerns over imminent extinction motivated a nuclear DNA study of the species in its largest continuous subpopulation in the eastern Mediterranean Sea. Despite recent evidence of partial subpopulation recovery, we demonstrate that there is no reason for complacency, as the species still shares several traits that are characteristic of a critically endangered species: Mediterranean monk seals in the eastern Mediterranean survive in three isolated and genetically depauperate population clusters, with small effective population sizes and high levels of inbreeding. Our results indicated male philopatry over short distances, which is unexpected for a polygynous mammal. Such a pattern may be explained by the species’ unique breeding behavior, in which males defend aquatic territories near breeding sites, while females are often forced to search for new pupping areas. Immediate action is necessary to reverse the downward spiral of population decline, inbreeding accumulation and loss of genetic diversity. We propose concrete conservation measures for the Mediterranean monk seal focusing on reducing anthropogenic threats, increasing the population size and genetic diversity, and thus improving the long-term prospects of survival. |
Tsaparis, Dimitris; Konstantinidis, Ioannis; Palandacic, Anja; Kalogianni, Eleni; Stoumboudi, Maria Th.; Barbieri, Roberta; Vardakas, Leonidas; Koutsikos, Nicholas; Tsigenopoulos, Costas S Hydrobiologia, 848 (5), pp. 1163–1176, 2021, ISSN: 0018-8158, 1573-5117. @article{tsaparis_dna_2021, title = {DNA barcoding provides new insights on the distribution, systematics and conservation of the freshwater genus Pelasgus (Cypriniformes: Cyprinidae) in Greece}, author = {Dimitris Tsaparis and Ioannis Konstantinidis and Anja Palandacic and Eleni Kalogianni and Maria Th. Stoumboudi and Roberta Barbieri and Leonidas Vardakas and Nicholas Koutsikos and Costas S Tsigenopoulos}, url = {http://link.springer.com/10.1007/s10750-021-04526-9}, doi = {10.1007/s10750-021-04526-9}, issn = {0018-8158, 1573-5117}, year = {2021}, date = {2021-03-01}, urldate = {2021-02-24}, journal = {Hydrobiologia}, volume = {848}, number = {5}, pages = {1163--1176}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Fanini, Lucia; Piscart, Christophe; Pranzini, Enzo; Kerbiriou, Christian; Viol, Isabelle Le; Pétillon, Julien Estuarine, Coastal and Shelf Science, 250 , pp. 107148, 2021, ISSN: 02727714. @article{fanini_extended_2021, title = {The extended concept of littoral active zone considering soft sediment shores as social-ecological systems, and an application to Brittany (North-Western France)}, author = {Lucia Fanini and Christophe Piscart and Enzo Pranzini and Christian Kerbiriou and Isabelle Le Viol and Julien Pétillon}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/02/Riechers-et-al.-2021-2.pdf https://linkinghub.elsevier.com/retrieve/pii/S0272771420308799 }, doi = {10.1016/j.ecss.2020.107148}, issn = {02727714}, year = {2021}, date = {2021-03-01}, urldate = {2021-02-02}, journal = {Estuarine, Coastal and Shelf Science}, volume = {250}, pages = {107148}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Corriero, Aldo; Wylie, Matthew J; Nyuji, Mitsuo; Zupa, Rosa; Mylonas, Constantinos C Reproduction of greater amberjack (Seriola dumerili) and other members of the family Carangidae Journal Article Rev Aquacult, pp. raq.12544, 2021, ISSN: 1753-5123, 1753-5131. @article{corriero_reproduction_2021, title = {Reproduction of greater amberjack (Seriola dumerili) and other members of the family Carangidae}, author = {Aldo Corriero and Matthew J Wylie and Mitsuo Nyuji and Rosa Zupa and Constantinos C Mylonas}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/03/2021-Corriero-RAQ-pre-print.pdf https://onlinelibrary.wiley.com/doi/10.1111/raq.12544}, doi = {10.1111/raq.12544}, issn = {1753-5123, 1753-5131}, year = {2021}, date = {2021-02-01}, urldate = {2021-02-24}, journal = {Rev Aquacult}, pages = {raq.12544}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Katsika, Lydia; Flores, Mario Huesca; Kotzamanis, Yannis; Estevez, Alicia; Chatzifotis, Stavros Animals, 11 (2), pp. 392, 2021. @article{katsika_understanding_2021, title = {Understanding the Interaction Effects between Dietary Lipid Content and Rearing Temperature on Growth Performance, Feed Utilization, and Fat Deposition of Sea Bass (Dicentrarchus labrax)}, author = {Lydia Katsika and Mario Huesca Flores and Yannis Kotzamanis and Alicia Estevez and Stavros Chatzifotis}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/02/2021-Katsika-animals-10.pdf https://www.mdpi.com/2076-2615/11/2/392}, doi = {10.3390/ani11020392}, year = {2021}, date = {2021-02-01}, urldate = {2021-02-05}, journal = {Animals}, volume = {11}, number = {2}, pages = {392}, abstract = {This study was conducted to elucidate the interaction effects of temperature and dietary lipid levels (2 × 2 factorial experiment) on the growth performance, muscle, and liver composition in adult farmed European sea bass (Dicentrarchus labrax). Two groups of fish (190 g; 60 fish per group) were distributed in 12 tanks in triplicates and kept at two different temperature regimes; one starting at 23 °C and then changed to 17 °C for 61 days, and the other starting at 17 °C and then changed to 23 °C for 39 days. Two commercial diets containing both textasciitilde44% crude protein but incorporating different dietary lipid levels, 16.5% (D16) and 20.0% (D20) (dry matter (DM)), were fed to the fish to apparent satiation; the type of diet fed to each fish group remained constant throughout the experiment. Final body weight, weight gain, and specific growth rate were significantly higher for the fish group held at 23 °C compared to the fish group at 17 °C (before the temperature changes), while the dietary fat content did not have any profound effect in both groups. Furthermore, the different temperature regimes did not affect muscle or liver composition, but, on the contrary, dietary lipids affected hepatosomatic, perivisceral fat, and visceral indexes. Feed conversion ratio and specific growth rate were not affected by the dietary lipid level. An interaction of temperature and dietary lipid content was observed in daily feed consumption (DFC) and final body weight (FBW).}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study was conducted to elucidate the interaction effects of temperature and dietary lipid levels (2 × 2 factorial experiment) on the growth performance, muscle, and liver composition in adult farmed European sea bass (Dicentrarchus labrax). Two groups of fish (190 g; 60 fish per group) were distributed in 12 tanks in triplicates and kept at two different temperature regimes; one starting at 23 °C and then changed to 17 °C for 61 days, and the other starting at 17 °C and then changed to 23 °C for 39 days. Two commercial diets containing both textasciitilde44% crude protein but incorporating different dietary lipid levels, 16.5% (D16) and 20.0% (D20) (dry matter (DM)), were fed to the fish to apparent satiation; the type of diet fed to each fish group remained constant throughout the experiment. Final body weight, weight gain, and specific growth rate were significantly higher for the fish group held at 23 °C compared to the fish group at 17 °C (before the temperature changes), while the dietary fat content did not have any profound effect in both groups. Furthermore, the different temperature regimes did not affect muscle or liver composition, but, on the contrary, dietary lipids affected hepatosomatic, perivisceral fat, and visceral indexes. Feed conversion ratio and specific growth rate were not affected by the dietary lipid level. An interaction of temperature and dietary lipid content was observed in daily feed consumption (DFC) and final body weight (FBW). |
Kogiannou, Dimitra; Nikoloudaki, Chrysanthi; Rigos, George Absorption and depletion of dietary administered praziquantel in greater amberjack, Seriola dumerili Journal Article Aquaculture, 535 , pp. 736354, 2021, ISSN: 00448486. @article{kogiannou_absorption_2021, title = {Absorption and depletion of dietary administered praziquantel in greater amberjack, Seriola dumerili}, author = {Dimitra Kogiannou and Chrysanthi Nikoloudaki and George Rigos}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848621000168}, doi = {10.1016/j.aquaculture.2021.736354}, issn = {00448486}, year = {2021}, date = {2021-01-01}, urldate = {2021-02-02}, journal = {Aquaculture}, volume = {535}, pages = {736354}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Mladineo, Ivona; Hrabar, Jerko; Trumbić, Željka; Manousaki, Tereza; Tsakogiannis, Alexandros; Taggart, John B; Tsigenopoulos, Costas S Pathogens, 10 (2), pp. 100, 2021, ISSN: 2076-0817. @article{mladineo_community_2021, title = {Community Parameters and Genome-Wide RAD-Seq Loci of Ceratothoa oestroides Imply Its Transfer between Farmed European Sea Bass and Wild Farm-Aggregating Fish}, author = {Ivona Mladineo and Jerko Hrabar and Željka Trumbić and Tereza Manousaki and Alexandros Tsakogiannis and John B Taggart and Costas S Tsigenopoulos}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/02/2020-Mladineo-Pathogens-7.pdf https://www.mdpi.com/2076-0817/10/2/100}, doi = {10.3390/pathogens10020100}, issn = {2076-0817}, year = {2021}, date = {2021-01-01}, urldate = {2021-02-02}, journal = {Pathogens}, volume = {10}, number = {2}, pages = {100}, abstract = {Wild fish assemblages that aggregate within commercial marine aquaculture sites for feeding and shelter have been considered as a primary source of pathogenic parasites vectored to farmed fish maintained in net pens at an elevated density. In order to evaluate whether Ceratothoa oestroides (Isopoda, Cymothoidae), a generalist and pestilent isopod that is frequently found in Adriatic and Greek stocks of farmed European sea bass (Dicentrarchus labrax), transfers between wild and farmed fish, a RAD-Seq (restriction-site-associated DNA sequencing)-mediated genetic screening approach was employed. The double-digest RAD-Seq of 310 C. oestroides specimens collected from farmed European sea bass (138) and different wild farm-aggregating fish (172) identified 313 robust SNPs that evidenced a close genetic relatedness between the “wild” and “farmed” genotypes. ddRAD-Seq proved to be an effective method for detecting the discrete genetic structuring of C. oestroides and genotype intermixing between two populations. The parasite prevalence in the farmed sea bass was 1.02%, with a mean intensity of 2.0 and mean abundance of 0.02, while in the wild fish, the prevalence was 8.1%; the mean intensity, 1.81; and the mean abundance, 0.15. Such differences are likely a consequence of human interventions during the farmed fish’s rearing cycle that, nevertheless, did not affect the transfer of C. oestroides.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Wild fish assemblages that aggregate within commercial marine aquaculture sites for feeding and shelter have been considered as a primary source of pathogenic parasites vectored to farmed fish maintained in net pens at an elevated density. In order to evaluate whether Ceratothoa oestroides (Isopoda, Cymothoidae), a generalist and pestilent isopod that is frequently found in Adriatic and Greek stocks of farmed European sea bass (Dicentrarchus labrax), transfers between wild and farmed fish, a RAD-Seq (restriction-site-associated DNA sequencing)-mediated genetic screening approach was employed. The double-digest RAD-Seq of 310 C. oestroides specimens collected from farmed European sea bass (138) and different wild farm-aggregating fish (172) identified 313 robust SNPs that evidenced a close genetic relatedness between the “wild” and “farmed” genotypes. ddRAD-Seq proved to be an effective method for detecting the discrete genetic structuring of C. oestroides and genotype intermixing between two populations. The parasite prevalence in the farmed sea bass was 1.02%, with a mean intensity of 2.0 and mean abundance of 0.02, while in the wild fish, the prevalence was 8.1%; the mean intensity, 1.81; and the mean abundance, 0.15. Such differences are likely a consequence of human interventions during the farmed fish’s rearing cycle that, nevertheless, did not affect the transfer of C. oestroides. |
Krick, Madoka Vera; Desmarais, Erick; Samaras, Athanasios; Guéret, Elise; Dimitroglou, Arkadios; Pavlidis, Michalis; Tsigenopoulos, Costas; Guinand, Bruno Family-effects in the epigenomic response of red blood cells to a challenge test in the European sea bass (Dicentrarchus labrax, L.) Journal Article BMC Genomics, 22 (1), pp. 111, 2021, ISSN: 1471-2164. @article{krick_family-effects_2021, title = {Family-effects in the epigenomic response of red blood cells to a challenge test in the European sea bass (Dicentrarchus labrax, L.)}, author = {Madoka Vera Krick and Erick Desmarais and Athanasios Samaras and Elise Guéret and Arkadios Dimitroglou and Michalis Pavlidis and Costas Tsigenopoulos and Bruno Guinand}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/02/2021-Krick-2021_Family-effects-in-sb-BMG-Genomics-14.pdf https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-021-07420-9}, doi = {10.1186/s12864-021-07420-9}, issn = {1471-2164}, year = {2021}, date = {2021-01-01}, urldate = {2021-02-24}, journal = {BMC Genomics}, volume = {22}, number = {1}, pages = {111}, abstract = {Abstract Background In fish, minimally invasive blood sampling is widely used to monitor physiological stress with blood plasma biomarkers. As fish blood cells are nucleated, they might be a source a potential new markers derived from ‘omics technologies. We modified the epiGBS (epiGenotyping By Sequencing) technique to explore changes in genome-wide cytosine methylation in the red blood cells (RBCs) of challenged European sea bass ( Dicentrarchus labrax ), a species widely studied in both natural and farmed environments. Results We retrieved 501,108,033 sequencing reads after trimming, with a mean mapping efficiency of 73.0% (unique best hits). Minor changes in RBC methylome appeared to manifest after the challenge test and a family-effect was detected. Only fifty-seven differentially methylated cytosines (DMCs) close to 51 distinct genes distributed on 17 of 24 linkage groups (LGs) were detected between RBCs of pre- and post-challenge individuals. Thirty-seven of these genes were previously reported as differentially expressed in the brain of zebrafish, most of them involved in stress coping differences. While further investigation remains necessary, few DMC-related genes associated to the Brain Derived Neurotrophic Factor, a protein that favors stress adaptation and fear memory, appear relevant to integrate a centrally produced stress response in RBCs. Conclusion Our modified epiGBS protocol was powerful to analyze patterns of cytosine methylation in RBCs of D. labrax and to evaluate the impact of a challenge using minimally invasive blood samples . This study is the first approximation to identify epigenetic biomarkers of exposure to stress in fish.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Background In fish, minimally invasive blood sampling is widely used to monitor physiological stress with blood plasma biomarkers. As fish blood cells are nucleated, they might be a source a potential new markers derived from ‘omics technologies. We modified the epiGBS (epiGenotyping By Sequencing) technique to explore changes in genome-wide cytosine methylation in the red blood cells (RBCs) of challenged European sea bass ( Dicentrarchus labrax ), a species widely studied in both natural and farmed environments. Results We retrieved 501,108,033 sequencing reads after trimming, with a mean mapping efficiency of 73.0% (unique best hits). Minor changes in RBC methylome appeared to manifest after the challenge test and a family-effect was detected. Only fifty-seven differentially methylated cytosines (DMCs) close to 51 distinct genes distributed on 17 of 24 linkage groups (LGs) were detected between RBCs of pre- and post-challenge individuals. Thirty-seven of these genes were previously reported as differentially expressed in the brain of zebrafish, most of them involved in stress coping differences. While further investigation remains necessary, few DMC-related genes associated to the Brain Derived Neurotrophic Factor, a protein that favors stress adaptation and fear memory, appear relevant to integrate a centrally produced stress response in RBCs. Conclusion Our modified epiGBS protocol was powerful to analyze patterns of cytosine methylation in RBCs of D. labrax and to evaluate the impact of a challenge using minimally invasive blood samples . This study is the first approximation to identify epigenetic biomarkers of exposure to stress in fish. |
Bravakos, Panos; Mandalakis, Manolis; Nomikou, Paraskevi; Anastasiou, Thekla I; Kristoffersen, Jon Bent; Stavroulaki, Melanthia; Kilias, Stephanos; Kotoulas, Georgios; Magoulas, Antonios; Polymenakou, Paraskevi N Genomic adaptation of Pseudomonas strains to acidity and antibiotics in hydrothermal vents at Kolumbo submarine volcano, Greece Journal Article Sci Rep, 11 (1), pp. 1336, 2021, ISSN: 2045-2322. @article{bravakos_genomic_2021, title = {Genomic adaptation of Pseudomonas strains to acidity and antibiotics in hydrothermal vents at Kolumbo submarine volcano, Greece}, author = {Panos Bravakos and Manolis Mandalakis and Paraskevi Nomikou and Thekla I Anastasiou and Jon Bent Kristoffersen and Melanthia Stavroulaki and Stephanos Kilias and Georgios Kotoulas and Antonios Magoulas and Paraskevi N Polymenakou}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/02/2021-Bravakos-SciReport-4.pdf http://www.nature.com/articles/s41598-020-79359-y}, doi = {10.1038/s41598-020-79359-y}, issn = {2045-2322}, year = {2021}, date = {2021-01-01}, urldate = {2021-02-02}, journal = {Sci Rep}, volume = {11}, number = {1}, pages = {1336}, abstract = {Abstract Although the rise of antibiotic and multidrug resistant bacteria is one of the biggest current threats to human health, our understanding of the mechanisms involved in antibiotic resistance selection remains scarce. We performed whole genome sequencing of 21 Pseudomonas strains, previously isolated from an active submarine volcano of Greece, the Kolumbo volcano. Our goal was to identify the genetic basis of the enhanced co-tolerance to antibiotics and acidity of these Pseudomonas strains. Pangenome analysis identified 10,908 Gene Clusters (GCs). It revealed that the numbers of phage-related GCs and sigma factors, which both provide the mechanisms of adaptation to environmental stressors, were much higher in the high tolerant Pseudomonas strains compared to the rest ones. All identified GCs of these strains were associated with antimicrobial and multidrug resistance. The present study provides strong evidence that the CO 2 -rich seawater of the volcano associated with low pH might be a reservoir of microorganisms carrying multidrug efflux-mediated systems and pumps. We, therefore, suggest further studies of other extreme environments (or ecosystems) and their associated physicochemical parameters (or factors) in the rise of antibiotic resistance.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Although the rise of antibiotic and multidrug resistant bacteria is one of the biggest current threats to human health, our understanding of the mechanisms involved in antibiotic resistance selection remains scarce. We performed whole genome sequencing of 21 Pseudomonas strains, previously isolated from an active submarine volcano of Greece, the Kolumbo volcano. Our goal was to identify the genetic basis of the enhanced co-tolerance to antibiotics and acidity of these Pseudomonas strains. Pangenome analysis identified 10,908 Gene Clusters (GCs). It revealed that the numbers of phage-related GCs and sigma factors, which both provide the mechanisms of adaptation to environmental stressors, were much higher in the high tolerant Pseudomonas strains compared to the rest ones. All identified GCs of these strains were associated with antimicrobial and multidrug resistance. The present study provides strong evidence that the CO 2 -rich seawater of the volcano associated with low pH might be a reservoir of microorganisms carrying multidrug efflux-mediated systems and pumps. We, therefore, suggest further studies of other extreme environments (or ecosystems) and their associated physicochemical parameters (or factors) in the rise of antibiotic resistance. |
Makridis, Pavlos; Kokou, Fotini; Bournakas, Christos; Papandroulakis, Nikos; Sarropoulou, Elena Microorganisms, 9 (1), pp. 128, 2021, ISSN: 2076-2607. @article{makridis_isolation_2021, title = {Isolation of Phaeobacter sp. from Larvae of Atlantic Bonito (Sarda sarda) in a Mesocosmos Unit, and Its Use for the Rearing of European Seabass Larvae (Dicentrarchus labrax L.)}, author = {Pavlos Makridis and Fotini Kokou and Christos Bournakas and Nikos Papandroulakis and Elena Sarropoulou}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/02/2021_2-Makridis-MICROORGANISMS.pdf https://www.mdpi.com/2076-2607/9/1/128}, doi = {10.3390/microorganisms9010128}, issn = {2076-2607}, year = {2021}, date = {2021-01-01}, urldate = {2021-02-02}, journal = {Microorganisms}, volume = {9}, number = {1}, pages = {128}, abstract = {The target of this study was to use indigenous probiotic bacteria in the rearing of seabass larvae. A Phaeobacter sp. strain isolated from bonito yolk-sac larvae (Sarda sarda) and identified by amplification of 16S rDNA showed in vitro inhibition against Vibrio anguillarum. This Phaeobacter sp. strain was used in the rearing of seabass larvae (Dicentrarchus labrax L.) in a large-scale trial. The survival of seabass after 60 days of rearing and the specific growth rate at the late exponential growth phase were significantly higher in the treatment receiving probiotics (p textless 0.05). Microbial community richness as determined by denaturing gradient gel electrophoresis (DGGE) showed an increase in bacterial diversity with fish development. Changes associated with the administration of probiotics were observed 11 and 18 days after hatching but were not apparent after probiotic administration stopped. In a small challenge experiment, seabass larvae from probiotic treatment showed increased survival (p textless 0.05) after experimental infection with a mild pathogen (Vibrio harveyi). Overall, our results showed that the use of an indigenous probiotic strain had a beneficial impact on larval rearing in industry-like conditions.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The target of this study was to use indigenous probiotic bacteria in the rearing of seabass larvae. A Phaeobacter sp. strain isolated from bonito yolk-sac larvae (Sarda sarda) and identified by amplification of 16S rDNA showed in vitro inhibition against Vibrio anguillarum. This Phaeobacter sp. strain was used in the rearing of seabass larvae (Dicentrarchus labrax L.) in a large-scale trial. The survival of seabass after 60 days of rearing and the specific growth rate at the late exponential growth phase were significantly higher in the treatment receiving probiotics (p textless 0.05). Microbial community richness as determined by denaturing gradient gel electrophoresis (DGGE) showed an increase in bacterial diversity with fish development. Changes associated with the administration of probiotics were observed 11 and 18 days after hatching but were not apparent after probiotic administration stopped. In a small challenge experiment, seabass larvae from probiotic treatment showed increased survival (p textless 0.05) after experimental infection with a mild pathogen (Vibrio harveyi). Overall, our results showed that the use of an indigenous probiotic strain had a beneficial impact on larval rearing in industry-like conditions. |
Pisera, Andrzej; Gerovasileiou, Vasilis Lithistid Demosponges of Deep-Water Origin in Marine Caves of the North-Eastern Mediterranean Sea Journal Article Frontiers in Marine Science, 8 , 2021, ISSN: 2296-7745. @article{pisera_lithistid_2021, title = {Lithistid Demosponges of Deep-Water Origin in Marine Caves of the North-Eastern Mediterranean Sea}, author = {Andrzej Pisera and Vasilis Gerovasileiou}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/02/2021-Gerovasileiou-Frontiers-in-Marine-Science-11.pdf https://www.frontiersin.org/articles/10.3389/fmars.2021.630900/full}, doi = {10.3389/fmars.2021.630900}, issn = {2296-7745}, year = {2021}, date = {2021-01-01}, urldate = {2021-02-05}, journal = {Frontiers in Marine Science}, volume = {8}, abstract = {Desmas-bearing demosponges known as lithistids have heavily silicified skeleton and occur typically in bathyal environments of warm and tropical areas but may be found in certain shallow marine caves. Here we report, for the first time two lithistid species, i.e. Neophrissospongia endoumensis, and N. cf. nana, that were earlier known from Western Mediterranean marine caves, from four marine caves in the Aegean Sea (north-eastern Mediterranean), and their congener N. nolitangere from deep waters (ca. 300 m) of the same ecoregion. All marine caves, and sections within these caves, where lithistids occur, have freshwater springs. We interpret this surprising association between lithistids and freshwater input by elevated concentration of silica in water in cave sections where such springs occur, being 8–11 times higher in comparison with shallow water outside caves, and comparable to that of deep waters, that promoted lithistids’ development. One of the studied caves harbored an abundant population of N. endoumensis which formed large masses. The age estimation of these lithistids, based on known growth rate of related deep-water sponges, suggest that they could be 769–909 years old in the case of the largest specimen observed, about 100 cm large. These sponges could have colonized the caves from adjacent deep-water areas not earlier than 7,000–3,000 years ago, after the last glaciation, because earlier they were emerged. High variability of spicules, especially microscleres, and underdevelopment of megascleres may be related to silicic acid concentration.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Desmas-bearing demosponges known as lithistids have heavily silicified skeleton and occur typically in bathyal environments of warm and tropical areas but may be found in certain shallow marine caves. Here we report, for the first time two lithistid species, i.e. Neophrissospongia endoumensis, and N. cf. nana, that were earlier known from Western Mediterranean marine caves, from four marine caves in the Aegean Sea (north-eastern Mediterranean), and their congener N. nolitangere from deep waters (ca. 300 m) of the same ecoregion. All marine caves, and sections within these caves, where lithistids occur, have freshwater springs. We interpret this surprising association between lithistids and freshwater input by elevated concentration of silica in water in cave sections where such springs occur, being 8–11 times higher in comparison with shallow water outside caves, and comparable to that of deep waters, that promoted lithistids’ development. One of the studied caves harbored an abundant population of N. endoumensis which formed large masses. The age estimation of these lithistids, based on known growth rate of related deep-water sponges, suggest that they could be 769–909 years old in the case of the largest specimen observed, about 100 cm large. These sponges could have colonized the caves from adjacent deep-water areas not earlier than 7,000–3,000 years ago, after the last glaciation, because earlier they were emerged. High variability of spicules, especially microscleres, and underdevelopment of megascleres may be related to silicic acid concentration. |
Fioravanti, Maria Letizia; Gustinelli, Andrea; Rigos, George; Buchmann, Kurt; Caffara, Monica; Pascual, Santiago; Pardo, Miguel Ángel Negligible risk of zoonotic anisakid nematodes in farmed fish from European mariculture, 2016 to 2018 Journal Article Eurosurveillance, 26 (2), 2021, ISSN: 1560-7917. @article{fioravanti_negligible_2021, title = {Negligible risk of zoonotic anisakid nematodes in farmed fish from European mariculture, 2016 to 2018}, author = {Maria Letizia Fioravanti and Andrea Gustinelli and George Rigos and Kurt Buchmann and Monica Caffara and Santiago Pascual and Miguel Ángel Pardo}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/02/2021-Fioravanti-Eurosurv-5.pdf https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2021.26.2.1900717}, doi = {10.2807/1560-7917.ES.2021.26.2.1900717}, issn = {1560-7917}, year = {2021}, date = {2021-01-01}, urldate = {2021-02-02}, journal = {Eurosurveillance}, volume = {26}, number = {2}, abstract = {Background The increasing demand for raw or undercooked fish products, supplied by both aquaculture and fisheries, raises concerns about the transmission risk to humans of zoonotic fish parasites. This has led to the current European Union (EU) Regulation No 1276/2011 amending Annex III of Regulation (EC) No 853/2004 and mandating a freezing treatment of such products. Zoonotic parasites, particularly anisakid larvae, have been well documented in wild fish. Data on their presence in European aquaculture products, however, are still scarce, except for Atlantic salmon ( Salmo salar ), where the zoonotic risk was assessed as negligible, exempting it from freezing treatment. Aim To evaluate the zoonotic Anisakidae parasite risk in European farmed marine fish other than Atlantic salmon. Methods From 2016 to 2018 an observational parasitological survey was undertaken on 6,549 farmed fish including 2,753 gilthead seabream ( Sparus aurata ), 2,761 European seabass ( Dicentrarchus labrax ) and 1,035 turbot ( Scophthalmus maximus ) from 14 farms in Italy, Spain and Greece. Furthermore, 200 rainbow trout ( Oncorhynchus mykiss ) sea-caged in Denmark, as well as 352 seabream and 290 seabass imported in Italy and Spain from other countries were examined. Fish were subjected to visual inspection and candling. Fresh visceral organs/fillet samples were artificially digested or UV pressed and visually examined for zoonotic anisakid larvae. Results No zoonotic parasites were found in any of the fish investigated. Conclusions The risk linked to zoonotic Anisakidae in the examined fish species from European mariculture appears negligible. This study laid the groundwork for considerations to amend the current EU regulation.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Background The increasing demand for raw or undercooked fish products, supplied by both aquaculture and fisheries, raises concerns about the transmission risk to humans of zoonotic fish parasites. This has led to the current European Union (EU) Regulation No 1276/2011 amending Annex III of Regulation (EC) No 853/2004 and mandating a freezing treatment of such products. Zoonotic parasites, particularly anisakid larvae, have been well documented in wild fish. Data on their presence in European aquaculture products, however, are still scarce, except for Atlantic salmon ( Salmo salar ), where the zoonotic risk was assessed as negligible, exempting it from freezing treatment. Aim To evaluate the zoonotic Anisakidae parasite risk in European farmed marine fish other than Atlantic salmon. Methods From 2016 to 2018 an observational parasitological survey was undertaken on 6,549 farmed fish including 2,753 gilthead seabream ( Sparus aurata ), 2,761 European seabass ( Dicentrarchus labrax ) and 1,035 turbot ( Scophthalmus maximus ) from 14 farms in Italy, Spain and Greece. Furthermore, 200 rainbow trout ( Oncorhynchus mykiss ) sea-caged in Denmark, as well as 352 seabream and 290 seabass imported in Italy and Spain from other countries were examined. Fish were subjected to visual inspection and candling. Fresh visceral organs/fillet samples were artificially digested or UV pressed and visually examined for zoonotic anisakid larvae. Results No zoonotic parasites were found in any of the fish investigated. Conclusions The risk linked to zoonotic Anisakidae in the examined fish species from European mariculture appears negligible. This study laid the groundwork for considerations to amend the current EU regulation. |
Riechers, Maraja; Fanini, Lucia; Apicella, Annalisa; Galván, Carolina Batista; Blondel, Elise; Espiña, Begoña; Kefer, Simone; Keroullé, Tristan; Klun, Katja; Pereira, Tania R; Ronchi, Francesca; Rodríguez, Pedro Ruiz; Sardon, Haritz; Silva, Alexandra Viana; Stulgis, Maris; Ibarra-González, Nagore Plastics in our ocean as transdisciplinary challenge Journal Article Marine Pollution Bulletin, 164 , pp. 112051, 2021, ISSN: 0025326X. @article{riechers_plastics_2021, title = {Plastics in our ocean as transdisciplinary challenge}, author = {Maraja Riechers and Lucia Fanini and Annalisa Apicella and Carolina Batista Galván and Elise Blondel and Begoña Espiña and Simone Kefer and Tristan Keroullé and Katja Klun and Tania R Pereira and Francesca Ronchi and Pedro Ruiz Rodríguez and Haritz Sardon and Alexandra Viana Silva and Maris Stulgis and Nagore Ibarra-González}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0025326X21000850}, doi = {10.1016/j.marpolbul.2021.112051}, issn = {0025326X}, year = {2021}, date = {2021-01-01}, urldate = {2021-02-24}, journal = {Marine Pollution Bulletin}, volume = {164}, pages = {112051}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Karagiannidis, Efstratios; Papazoglou, Andreas S; Stalikas, Nikolaos; Deda, Olga; Panteris, Eleftherios; Begou, Olga; Sofidis, Georgios; Moysidis, Dimitrios V; Kartas, Anastasios; Chatzinikolaou, Evangelia; Keklikoglou, Kleoniki; Bompoti, Andreana; Gika, Helen; Theodoridis, Georgios; Sianos, Georgios Serum Ceramides as Prognostic Biomarkers of Large Thrombus Burden in Patients with STEMI: A Micro-Computed Tomography Study Journal Article Journal of Personalized Medicine, 11 (2), pp. 89, 2021. @article{karagiannidis_serum_2021, title = {Serum Ceramides as Prognostic Biomarkers of Large Thrombus Burden in Patients with STEMI: A Micro-Computed Tomography Study}, author = {Efstratios Karagiannidis and Andreas S Papazoglou and Nikolaos Stalikas and Olga Deda and Eleftherios Panteris and Olga Begou and Georgios Sofidis and Dimitrios V Moysidis and Anastasios Kartas and Evangelia Chatzinikolaou and Kleoniki Keklikoglou and Andreana Bompoti and Helen Gika and Georgios Theodoridis and Georgios Sianos}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/02/2021-Karagiannidis-Chatzinikolaou-Keklikoglou-JPMed-9.pdf https://www.mdpi.com/2075-4426/11/2/89}, doi = {10.3390/jpm11020089}, year = {2021}, date = {2021-01-01}, urldate = {2021-02-05}, journal = {Journal of Personalized Medicine}, volume = {11}, number = {2}, pages = {89}, abstract = {ST-elevation myocardial infarction (STEMI) remains one of the leading causes of mortality worldwide. The identification of novel metabolic and imaging biomarkers could unveil key pathophysiological mechanisms at the molecular level and promote personalized care in patients with acute coronary syndromes. We studied 38 patients with STEMI who underwent primary percutaneous coronary intervention and thrombus aspiration. We sought to correlate serum ceramide levels with micro-CT quantified aspirated thrombus volume and relevant angiographic outcomes, including modified TIMI thrombus grade and pre- or post-procedural TIMI flow. Higher ceramide C16:0 levels were significantly but weakly correlated with larger aspirated thrombus volume (Spearman r = 0.326}, keywords = {}, pubstate = {published}, tppubtype = {article} } ST-elevation myocardial infarction (STEMI) remains one of the leading causes of mortality worldwide. The identification of novel metabolic and imaging biomarkers could unveil key pathophysiological mechanisms at the molecular level and promote personalized care in patients with acute coronary syndromes. We studied 38 patients with STEMI who underwent primary percutaneous coronary intervention and thrombus aspiration. We sought to correlate serum ceramide levels with micro-CT quantified aspirated thrombus volume and relevant angiographic outcomes, including modified TIMI thrombus grade and pre- or post-procedural TIMI flow. Higher ceramide C16:0 levels were significantly but weakly correlated with larger aspirated thrombus volume (Spearman r = 0.326 |
Hermi, Fatma; Gómez-Abellán, Victoria; Pérez-Oliva, Ana B; García-Moreno, Diana; López-Muñoz, Azucena; Sarropoulou, Elena; Arizcun, Marta; Ridha, Oueslati; Mulero, Victoriano; Sepulcre, María P Developmental & Comparative Immunology, 114 , pp. 103803, 2021, ISSN: 0145305X. @article{hermi_molecular_2021, title = {The molecular, functional and phylogenetic characterization of PGE2 receptors reveals their different roles in the immune response of the teleost fish gilthead seabream (Sparus aurata L.)}, author = {Fatma Hermi and Victoria Gómez-Abellán and Ana B Pérez-Oliva and Diana García-Moreno and Azucena López-Muñoz and Elena Sarropoulou and Marta Arizcun and Oueslati Ridha and Victoriano Mulero and María P Sepulcre}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0145305X2030358X}, doi = {10.1016/j.dci.2020.103803}, issn = {0145305X}, year = {2021}, date = {2021-01-01}, urldate = {2020-08-23}, journal = {Developmental & Comparative Immunology}, volume = {114}, pages = {103803}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Maidanou, Maria; Koulouri, Panayota; Karachle, Paraskevi K; Arvanitidis, Christos; Koutsoubas, Drosos; Dounas, Costas Trophic Diversity of a Fish Community Associated with a Caulerpa prolifera (Forsskål) Meadow in a Shallow Semi-Enclosed Embayment Journal Article JMSE, 9 (2), pp. 165, 2021, ISSN: 2077-1312. @article{maidanou_trophic_2021, title = {Trophic Diversity of a Fish Community Associated with a Caulerpa prolifera (Forsskål) Meadow in a Shallow Semi-Enclosed Embayment}, author = {Maria Maidanou and Panayota Koulouri and Paraskevi K Karachle and Christos Arvanitidis and Drosos Koutsoubas and Costas Dounas}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/02/2021-Maidanou-JMSE-12.pdf https://www.mdpi.com/2077-1312/9/2/165}, doi = {10.3390/jmse9020165}, issn = {2077-1312}, year = {2021}, date = {2021-01-01}, urldate = {2021-02-24}, journal = {JMSE}, volume = {9}, number = {2}, pages = {165}, abstract = {This study investigates the trophic diversity of fishes living in a meadow of Caulerpa prolifera on a bimonthly basis between May 2006 and April 2007 in a semi-enclosed coastal marine ecosystem of the Mediterranean Sea (Elounda Bay, Crete Island). The study area is shallow and protected from waves, and it is covered by a C. prolifera bed, characterized by high organic input and a highly diverse macrobenthic community. Feeding patterns of the fish, investigated on the basis of stomach content analyses, were described in terms of numerical abundance and frequency of occurrence of prey taxa. A total of 1642 fish individuals, belonging to 17 species, were examined. In total, 45,674 prey individuals were identified belonging to 110 prey taxa, most of which were Malacostraca including their larvae and Copepoda (41,175 individuals identified to 71 taxa). Four different trophic groups were identified: herbivorous, pelagic, benthic (hyperbenthic) and piscivorous. Trophic diversity patterns of the fish species studied were also compared to the relative availability of macrobenthic and zooplanktonic taxa during the same period in the study area. The coexistence of many different, mostly benthic but also pelagic, fishes and their juveniles implies their high trophic flexibility, which is probably important for their survival in this particular habitat. Results of the present study provide basic knowledge on trophic diversity and interactions in the marine ecosystem and, therefore, some evidence as to the protection value of this particular habitat, which is essential for the implementation of a multispecies approach to decision-makers and managers of fisheries sources of the region.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study investigates the trophic diversity of fishes living in a meadow of Caulerpa prolifera on a bimonthly basis between May 2006 and April 2007 in a semi-enclosed coastal marine ecosystem of the Mediterranean Sea (Elounda Bay, Crete Island). The study area is shallow and protected from waves, and it is covered by a C. prolifera bed, characterized by high organic input and a highly diverse macrobenthic community. Feeding patterns of the fish, investigated on the basis of stomach content analyses, were described in terms of numerical abundance and frequency of occurrence of prey taxa. A total of 1642 fish individuals, belonging to 17 species, were examined. In total, 45,674 prey individuals were identified belonging to 110 prey taxa, most of which were Malacostraca including their larvae and Copepoda (41,175 individuals identified to 71 taxa). Four different trophic groups were identified: herbivorous, pelagic, benthic (hyperbenthic) and piscivorous. Trophic diversity patterns of the fish species studied were also compared to the relative availability of macrobenthic and zooplanktonic taxa during the same period in the study area. The coexistence of many different, mostly benthic but also pelagic, fishes and their juveniles implies their high trophic flexibility, which is probably important for their survival in this particular habitat. Results of the present study provide basic knowledge on trophic diversity and interactions in the marine ecosystem and, therefore, some evidence as to the protection value of this particular habitat, which is essential for the implementation of a multispecies approach to decision-makers and managers of fisheries sources of the region. |
2020 |
Kotzamanis, Yannis; Tsironi, Theofania; Brezas, Andreas; Grigorakis, Kriton; Ilia, Vassiliki; Vatsos, Ioannis; Romano, Nicholas; van Eys, Jan; Kumar, Vikas Scientific Reports, 10 (1), pp. 12294, 2020, ISSN: 2045-2322. @article{kotzamanis_high_2020, title = {High taurine supplementation in plant protein-based diets improves growth and organoleptic characteristics of European seabass (Dicentrarchus labrax)}, author = {Yannis Kotzamanis and Theofania Tsironi and Andreas Brezas and Kriton Grigorakis and Vassiliki Ilia and Ioannis Vatsos and Nicholas Romano and Jan van Eys and Vikas Kumar}, url = {http://www.nature.com/articles/s41598-020-69014-x}, doi = {10.1038/s41598-020-69014-x}, issn = {2045-2322}, year = {2020}, date = {2020-12-01}, urldate = {2020-08-10}, journal = {Scientific Reports}, volume = {10}, number = {1}, pages = {12294}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Zenetos, Argyro; Karachle, Paraskevi K; Corsini-Foka, Maria; Gerovasileiou, Vasilis; Simboura, Nomiki; Xentidis, Nikolas Jason; Tsiamis, Konstantinos Mediterranean Marine Science, 21 (3), pp. 775, 2020, ISSN: 1791-6763, 1108-393X. @article{zenetos_is_2020, title = {Is the trend in new introductions of marine non-indigenous species a reliable criterion for assessing good environmental status? Τhe case study of Greece}, author = {Argyro Zenetos and Paraskevi K Karachle and Maria Corsini-Foka and Vasilis Gerovasileiou and Nomiki Simboura and Nikolas Jason Xentidis and Konstantinos Tsiamis}, url = {https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/25136}, doi = {10.12681/mms.25136}, issn = {1791-6763, 1108-393X}, year = {2020}, date = {2020-12-01}, urldate = {2020-12-08}, journal = {Mediterranean Marine Science}, volume = {21}, number = {3}, pages = {775}, abstract = {This study presents the updated status of marine non-indigenous species (NIS) distribution in Greece and investigates trends in new NIS introductions, at both national and subnational level, during 1970-2017. The overall picture shows an increase in new introductions from the 1970s to 2017. The number of unaided introduced species (mainly Lessepsian immigrants) followed an upward trend in the South Aegean Sea until 2017. Similarly, the number of NIS associated with transport-stowaway (NIS introduced mainly via ballast and boat hulls) followed an upward until 2017 in the South Aegean Sea, but also in the Hellenic Levantine coasts. However, these results are greatly affected by a monitoring bias, which appears to be the main factor influencing the number of new NIS introductions reported from Greece and its subnational areas. This monitoring bias, as well as the continuous influx of Lessepsian NIS into the Aegean Sea, constitutes a challenge for Greece as regards setting exact boundaries for areas with or without Good Environmental Status (GES), based on Descriptor 2, primary criterion C1 (D2C1), of the Marine Strategy Framework Directive (MSFD). Dedicated monitoring of marine NIS should be established and be constant in space, time and across taxonomic groups. Prioritization should be given to hot-spot areas of new NIS introductions, such as ports, aquaculture units and marine protected areas. This should be a prerequisite for applying the primary criterion D2C1 of the MSFD properly, at both national and subnational level. Finally, as regards the implementation of D2C1 of the MSDF and setting exact threshold values, we highlight the need for subregional and regional coordination in the Mediterranean.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study presents the updated status of marine non-indigenous species (NIS) distribution in Greece and investigates trends in new NIS introductions, at both national and subnational level, during 1970-2017. The overall picture shows an increase in new introductions from the 1970s to 2017. The number of unaided introduced species (mainly Lessepsian immigrants) followed an upward trend in the South Aegean Sea until 2017. Similarly, the number of NIS associated with transport-stowaway (NIS introduced mainly via ballast and boat hulls) followed an upward until 2017 in the South Aegean Sea, but also in the Hellenic Levantine coasts. However, these results are greatly affected by a monitoring bias, which appears to be the main factor influencing the number of new NIS introductions reported from Greece and its subnational areas. This monitoring bias, as well as the continuous influx of Lessepsian NIS into the Aegean Sea, constitutes a challenge for Greece as regards setting exact boundaries for areas with or without Good Environmental Status (GES), based on Descriptor 2, primary criterion C1 (D2C1), of the Marine Strategy Framework Directive (MSFD). Dedicated monitoring of marine NIS should be established and be constant in space, time and across taxonomic groups. Prioritization should be given to hot-spot areas of new NIS introductions, such as ports, aquaculture units and marine protected areas. This should be a prerequisite for applying the primary criterion D2C1 of the MSFD properly, at both national and subnational level. Finally, as regards the implementation of D2C1 of the MSDF and setting exact threshold values, we highlight the need for subregional and regional coordination in the Mediterranean. |
Karagiannidis, Efstratios; Konstantinidis, Nikolaos V; Sofidis, Georgios; Chatzinikolaou, Evangelia; Sianos, Georgios BMC Cardiovascular Disorders, 20 (1), pp. 125, 2020, ISSN: 1471-2261. @article{karagiannidis_rationale_2020, title = {Rationale and design of a prospective, observational study for the QUantitative EStimation of Thrombus burden in patients with ST-Elevation Myocardial Infarction using micro-computed tomography: the QUEST-STEMI trial}, author = {Efstratios Karagiannidis and Nikolaos V Konstantinidis and Georgios Sofidis and Evangelia Chatzinikolaou and Georgios Sianos}, url = {https://bmccardiovascdisord.biomedcentral.com/articles/10.1186/s12872-020-01393-5}, doi = {10.1186/s12872-020-01393-5}, issn = {1471-2261}, year = {2020}, date = {2020-12-01}, journal = {BMC Cardiovascular Disorders}, volume = {20}, number = {1}, pages = {125}, abstract = {Although the presence of thrombus in patients with ST-elevation myocardial infarction (STEMI) has been linked to adverse outcomes, routine thrombus aspiration has not been proven effective. A potential explanation is that these patients should be risk-stratified. Traditional clinical, laboratory and angiographic parameters used in clinical trials have been proven inadequate to classify patients. Aspirated thrombotic material characteristics might be an additional important parameter that has not yet been addressed. In this report, we aim to describe a methodological analysis of thrombus aspirated from coronary arteries during primary PCI using micro-Computed $textbackslashTau$omography (micro-CT). These data will be combined with traditional factors to develop a risk-stratification system with high discriminative power for these patients.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Although the presence of thrombus in patients with ST-elevation myocardial infarction (STEMI) has been linked to adverse outcomes, routine thrombus aspiration has not been proven effective. A potential explanation is that these patients should be risk-stratified. Traditional clinical, laboratory and angiographic parameters used in clinical trials have been proven inadequate to classify patients. Aspirated thrombotic material characteristics might be an additional important parameter that has not yet been addressed. In this report, we aim to describe a methodological analysis of thrombus aspirated from coronary arteries during primary PCI using micro-Computed $textbackslashTau$omography (micro-CT). These data will be combined with traditional factors to develop a risk-stratification system with high discriminative power for these patients. |
Mastoraki, Maria; Ferrándiz, Paula Mollá; Vardali, Sofia C; Kontodimas, Demetrius C; Kotzamanis, Yannis P; Gasco, Laura; Chatzifotis, Stavros; Antonopoulou, Efthimia Aquaculture, 528 , pp. 735511, 2020, ISSN: 00448486. @article{mastoraki_comparative_2020, title = {A comparative study on the effect of fish meal substitution with three different insect meals on growth, body composition and metabolism of European sea bass (Dicentrarchus labrax L.)}, author = {Maria Mastoraki and Paula Mollá Ferrándiz and Sofia C Vardali and Demetrius C Kontodimas and Yannis P Kotzamanis and Laura Gasco and Stavros Chatzifotis and Efthimia Antonopoulou}, url = {https://linkinghub.elsevier.com/retrieve/pii/S004484862030065X}, doi = {10.1016/j.aquaculture.2020.735511}, issn = {00448486}, year = {2020}, date = {2020-11-01}, urldate = {2020-07-06}, journal = {Aquaculture}, volume = {528}, pages = {735511}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Obst, Matthias; Exter, Katrina; Allcock, Louise A; Arvanitidis, Christos; Axberg, Alizz; Bustamante, Maria; Cancio, Ibon; Carreira-Flores, Diego; Chatzinikolaou, Eva; Chatzigeorgiou, Giorgos; Chrismas, Nathan; Clark, Melody S; Comtet, Thierry; Dailianis, Thanos; Davies, Neil; Deneudt, Klaas; de Cerio, Oihane Diaz; Fortič, Ana; Gerovasileiou, Vasilis; Hablützel, Pascal I; Keklikoglou, Kleoniki; Kotoulas, Georgios; Lasota, Rafal; Leite, Barbara R; Loisel, Stéphane; Lévêque, Laurent; Levy, Liraz; Malachowicz, Magdalena; Mavrič, Borut; Meyer, Christopher; Mortelmans, Jonas; Norkko, Joanna; Pade, Nicolas; Power, Anne Marie; Ramšak, Andreja; Reiss, Henning; Solbakken, Jostein; Staehr, Peter A; Sundberg, Per; Thyrring, Jakob; Troncoso, Jesus S; Viard, Frédérique; Wenne, Roman; Yperifanou, Eleni Ioanna; Zbawicka, Malgorzata; Pavloudi, Christina A Marine Biodiversity Observation Network for Genetic Monitoring of Hard-Bottom Communities (ARMS-MBON) Journal Article Frontiers in Marine Science, 7 , pp. 572680, 2020, ISSN: 2296-7745. @article{obst_marine_2020, title = {A Marine Biodiversity Observation Network for Genetic Monitoring of Hard-Bottom Communities (ARMS-MBON)}, author = {Matthias Obst and Katrina Exter and Louise A Allcock and Christos Arvanitidis and Alizz Axberg and Maria Bustamante and Ibon Cancio and Diego Carreira-Flores and Eva Chatzinikolaou and Giorgos Chatzigeorgiou and Nathan Chrismas and Melody S Clark and Thierry Comtet and Thanos Dailianis and Neil Davies and Klaas Deneudt and Oihane Diaz de Cerio and Ana Fortič and Vasilis Gerovasileiou and Pascal I Hablützel and Kleoniki Keklikoglou and Georgios Kotoulas and Rafal Lasota and Barbara R Leite and Stéphane Loisel and Laurent Lévêque and Liraz Levy and Magdalena Malachowicz and Borut Mavrič and Christopher Meyer and Jonas Mortelmans and Joanna Norkko and Nicolas Pade and Anne Marie Power and Andreja Ramšak and Henning Reiss and Jostein Solbakken and Peter A Staehr and Per Sundberg and Jakob Thyrring and Jesus S Troncoso and Frédérique Viard and Roman Wenne and Eleni Ioanna Yperifanou and Malgorzata Zbawicka and Christina Pavloudi}, url = {https://www.frontiersin.org/articles/10.3389/fmars.2020.572680/full}, doi = {10.3389/fmars.2020.572680}, issn = {2296-7745}, year = {2020}, date = {2020-11-01}, urldate = {2020-12-08}, journal = {Frontiers in Marine Science}, volume = {7}, pages = {572680}, abstract = {Marine hard-bottom communities are undergoing severe change under the influence of multiple drivers, notably climate change, extraction of natural resources, pollution and eutrophication, habitat degradation, and invasive species. Monitoring marine biodiversity in such habitats is, however, challenging as it typically involves expensive, non-standardized, and often destructive sampling methods that limit its scalability. Differences in monitoring approaches furthermore hinders inter-comparison among monitoring programs. Here, we announce a Marine Biodiversity Observation Network (MBON) consisting of Autonomous Reef Monitoring Structures (ARMS) with the aim to assess the status and changes in benthic fauna with genomic-based methods, notably DNA metabarcoding, in combination with image-based identifications. This article presents the results of a 30-month pilot phase in which we established an operational and geographically expansive ARMS-MBON. The network currently consists of 20 observatories distributed across European coastal waters and the polar regions, in which 134 ARMS have been deployed to date. Sampling takes place annually, either as short-term deployments during the summer or as long-term deployments starting in spring. The pilot phase was used to establish a common set of standards for field sampling, genetic analysis, data management, and legal compliance, which are presented here. We also tested the potential of ARMS for combining genetic and image-based identification methods in comparative studies of benthic diversity, as well as for detecting non-indigenous species. Results show that ARMS are suitable for monitoring hard-bottom environments as they provide genetic data that can be continuously enriched, re-analyzed, and integrated with conventional data to document benthic community composition and detect non-indigenous species. Finally, we provide guidelines to expand the network and present a sustainability plan as part of the European Marine Biological Resource Centre ( www.embrc.eu ).}, keywords = {}, pubstate = {published}, tppubtype = {article} } Marine hard-bottom communities are undergoing severe change under the influence of multiple drivers, notably climate change, extraction of natural resources, pollution and eutrophication, habitat degradation, and invasive species. Monitoring marine biodiversity in such habitats is, however, challenging as it typically involves expensive, non-standardized, and often destructive sampling methods that limit its scalability. Differences in monitoring approaches furthermore hinders inter-comparison among monitoring programs. Here, we announce a Marine Biodiversity Observation Network (MBON) consisting of Autonomous Reef Monitoring Structures (ARMS) with the aim to assess the status and changes in benthic fauna with genomic-based methods, notably DNA metabarcoding, in combination with image-based identifications. This article presents the results of a 30-month pilot phase in which we established an operational and geographically expansive ARMS-MBON. The network currently consists of 20 observatories distributed across European coastal waters and the polar regions, in which 134 ARMS have been deployed to date. Sampling takes place annually, either as short-term deployments during the summer or as long-term deployments starting in spring. The pilot phase was used to establish a common set of standards for field sampling, genetic analysis, data management, and legal compliance, which are presented here. We also tested the potential of ARMS for combining genetic and image-based identification methods in comparative studies of benthic diversity, as well as for detecting non-indigenous species. Results show that ARMS are suitable for monitoring hard-bottom environments as they provide genetic data that can be continuously enriched, re-analyzed, and integrated with conventional data to document benthic community composition and detect non-indigenous species. Finally, we provide guidelines to expand the network and present a sustainability plan as part of the European Marine Biological Resource Centre ( www.embrc.eu ). |
Emmanouilidis, Alexandros; Unkel, Ingmar; Seguin, Joana; Keklikoglou, Kleoniki; Gianni, Eleni; Avramidis, Pavlos Application of Non-Destructive Techniques on a Varve Sediment Record from Vouliagmeni Coastal Lake, Eastern Gulf of Corinth, Greece Journal Article Applied Sciences, 10 (22), pp. 8273, 2020, ISSN: 2076-3417. @article{emmanouilidis_application_2020, title = {Application of Non-Destructive Techniques on a Varve Sediment Record from Vouliagmeni Coastal Lake, Eastern Gulf of Corinth, Greece}, author = {Alexandros Emmanouilidis and Ingmar Unkel and Joana Seguin and Kleoniki Keklikoglou and Eleni Gianni and Pavlos Avramidis}, url = {https://www.mdpi.com/2076-3417/10/22/8273}, doi = {10.3390/app10228273}, issn = {2076-3417}, year = {2020}, date = {2020-11-01}, urldate = {2020-11-23}, journal = {Applied Sciences}, volume = {10}, number = {22}, pages = {8273}, abstract = {During the last few decades, X-ray attenuation systems have been established as standard techniques in geosciences and as excellent scientific tools for the analysis of sedimentary facies and structures. In the present study, we use two non-destructive and high-resolution systems (computed tomography, X-ray fluorescence) to address all sedimentological facies and structural characteristics on a 6 m long, partly laminated sediment record, from Vouliagmeni lake, located at the eastern part of the Corinth Gulf, Greece. Vouliagmeni lake is the deepest coastal lake in Greece, and its location is of great importance since it is located in one of the most tectonically active regions in the world. The chronological framework of the retrieved sediment sequence spans the last 12,000 years, with alternations of laminated and non-laminated sections. The annual accumulation of the laminated sequences was determined through the radionuclide concentration of 14 samples. Scanning tomography was performed with a medical CT scanner and a μCT scanner, aiming to compare the potentials and variations of both methods. Lamination boundaries, event layers (turbidites) and sediment deformations were distinguished through processing the extracted 3D rendered volumes, after applying ranges depending on Hounsfield (HU) values. Microscopic analysis revealed three main layer types in the varve sequences that were examined, attributing to summer/spring, autumn and winter deposits. Statistical analysis of the elemental composition, magnetic susceptibility (MS) measurements and HU values revealed three clusters/facies, reflecting climatic and in-lake hydrological changes. Cluster A emulates sedimentation during arid conditions, while Cluster B reflects humid conditions with increased precipitation and erosion. Cluster C represents sequences with homogenous Ca-rich sediment. Our proposed workflow highlights the possible correlation between the non-destructive variables that were measured, but also the variations and applications of each method and software used during this study.}, keywords = {}, pubstate = {published}, tppubtype = {article} } During the last few decades, X-ray attenuation systems have been established as standard techniques in geosciences and as excellent scientific tools for the analysis of sedimentary facies and structures. In the present study, we use two non-destructive and high-resolution systems (computed tomography, X-ray fluorescence) to address all sedimentological facies and structural characteristics on a 6 m long, partly laminated sediment record, from Vouliagmeni lake, located at the eastern part of the Corinth Gulf, Greece. Vouliagmeni lake is the deepest coastal lake in Greece, and its location is of great importance since it is located in one of the most tectonically active regions in the world. The chronological framework of the retrieved sediment sequence spans the last 12,000 years, with alternations of laminated and non-laminated sections. The annual accumulation of the laminated sequences was determined through the radionuclide concentration of 14 samples. Scanning tomography was performed with a medical CT scanner and a μCT scanner, aiming to compare the potentials and variations of both methods. Lamination boundaries, event layers (turbidites) and sediment deformations were distinguished through processing the extracted 3D rendered volumes, after applying ranges depending on Hounsfield (HU) values. Microscopic analysis revealed three main layer types in the varve sequences that were examined, attributing to summer/spring, autumn and winter deposits. Statistical analysis of the elemental composition, magnetic susceptibility (MS) measurements and HU values revealed three clusters/facies, reflecting climatic and in-lake hydrological changes. Cluster A emulates sedimentation during arid conditions, while Cluster B reflects humid conditions with increased precipitation and erosion. Cluster C represents sequences with homogenous Ca-rich sediment. Our proposed workflow highlights the possible correlation between the non-destructive variables that were measured, but also the variations and applications of each method and software used during this study. |
Costa, Leonardo Lopes; Zalmon, Ilana Rosental; Fanini, Lucia; Defeo, Omar Macroinvertebrates as indicators of human disturbances on sandy beaches: A global review Journal Article Ecological Indicators, 118 , pp. 106764, 2020, ISSN: 1470160X. @article{costa_macroinvertebrates_2020, title = {Macroinvertebrates as indicators of human disturbances on sandy beaches: A global review}, author = {Leonardo Lopes Costa and Ilana Rosental Zalmon and Lucia Fanini and Omar Defeo}, url = {https://linkinghub.elsevier.com/retrieve/pii/S1470160X20307020}, doi = {10.1016/j.ecolind.2020.106764}, issn = {1470160X}, year = {2020}, date = {2020-11-01}, urldate = {2020-09-17}, journal = {Ecological Indicators}, volume = {118}, pages = {106764}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Rosso, Antonietta; Gerovasileiou, Vasilis; Martino, Emanuela Di Really Onychocellids? Revisions and New Findings Increase the Astonishing Bryozoan Diversity of the Mediterranean Sea Journal Article Journal of Marine Science and Engineering, 8 (11), pp. 904, 2020, ISSN: 2077-1312. @article{rosso_really_2020, title = {Really Onychocellids? Revisions and New Findings Increase the Astonishing Bryozoan Diversity of the Mediterranean Sea}, author = {Antonietta Rosso and Vasilis Gerovasileiou and Emanuela Di Martino}, url = {https://www.mdpi.com/2077-1312/8/11/904}, doi = {10.3390/jmse8110904}, issn = {2077-1312}, year = {2020}, date = {2020-11-01}, urldate = {2020-12-08}, journal = {Journal of Marine Science and Engineering}, volume = {8}, number = {11}, pages = {904}, abstract = {Investigation of bryozoan faunas collected in two submarine caves in Lesvos Island, Aegean Sea revealed a great number of colonies of three species currently assigned to the cheilostome family Onychocellidae: Onychocella marioni Jullien, 1882, O. vibraculifera Neviani, 1895, and Smittipora disjuncta Canu & Bassler, 1930. All species were first described and subsequently recorded on several occasions, from the Mediterranean Sea, particularly from the Aegean Sea. The availability of this material provided the basis for more detailed observations and first scanning electron microscopy (SEM) study of some diagnostic characters, including ovicells and ancestrulae, for the well-known species, as well as a few colonies of a species left in open nomenclature (i.e., Onychocellidae sp. 1) in previous works. In this paper we (i) update the descriptions of these four species; (ii) resurrect the species Floridinella arculifera Canu & Bassler, 1927, which was previously synonymised with Caleschara minuta (Maplestone, 1909), suggesting for it the new combination Tretosina arculifera; (iii) and introduce the new genus Bryobifallax for S. disjuncta.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Investigation of bryozoan faunas collected in two submarine caves in Lesvos Island, Aegean Sea revealed a great number of colonies of three species currently assigned to the cheilostome family Onychocellidae: Onychocella marioni Jullien, 1882, O. vibraculifera Neviani, 1895, and Smittipora disjuncta Canu & Bassler, 1930. All species were first described and subsequently recorded on several occasions, from the Mediterranean Sea, particularly from the Aegean Sea. The availability of this material provided the basis for more detailed observations and first scanning electron microscopy (SEM) study of some diagnostic characters, including ovicells and ancestrulae, for the well-known species, as well as a few colonies of a species left in open nomenclature (i.e., Onychocellidae sp. 1) in previous works. In this paper we (i) update the descriptions of these four species; (ii) resurrect the species Floridinella arculifera Canu & Bassler, 1927, which was previously synonymised with Caleschara minuta (Maplestone, 1909), suggesting for it the new combination Tretosina arculifera; (iii) and introduce the new genus Bryobifallax for S. disjuncta. |
Bolgan, Marta; Pereira, Beatriz P; Crucianelli, Aurora; Mylonas, Constantinos C; Pousão-Ferreira, Pedro; Parmentier, Eric; Fonseca, Paulo J; Amorim, Clara M P Vocal repertoire and consistency of call features in the meagre Argyrosomous regius (Asso, 1801) Journal Article PLOS ONE, 15 (11), pp. e0241792, 2020, ISSN: 1932-6203. @article{bolgan_vocal_2020, title = {Vocal repertoire and consistency of call features in the meagre Argyrosomous regius (Asso, 1801)}, author = {Marta Bolgan and Beatriz P Pereira and Aurora Crucianelli and Constantinos C Mylonas and Pedro Pousão-Ferreira and Eric Parmentier and Paulo J Fonseca and Clara M P Amorim}, editor = {Dennis M Higgs}, url = {https://dx.plos.org/10.1371/journal.pone.0241792}, doi = {10.1371/journal.pone.0241792}, issn = {1932-6203}, year = {2020}, date = {2020-11-01}, urldate = {2020-12-08}, journal = {PLOS ONE}, volume = {15}, number = {11}, pages = {e0241792}, abstract = {Passive Acoustic Monitoring (PAM) is a non-intrusive and cost-effective method capable of providing high-resolution, long-term information on the status and health of vocal populations and communities. To successfully monitor the same species over wide geographical and temporal scales, it is necessary to characterise the range of sound variability, as well as the consistency of sound features between populations. The meagre ( Argyrosomus regius , Asso 1801) is an interesting case study because recent investigations suggest a wider vocal repertoire than previously described. In this study, meagre vocalizations were recorded and analysed from a variety of settings, ranging from rearing facilities to wild populations to provide a comprehensive characterisation of its vocal repertoire, while investigating the consistency of spawning sound features between populations. All sounds presented a similar acoustic structure in their basic unit ( i . e . the pulse), while an important variability was found in the number of pulses; the meagre can emit sounds made of one single pulse or many pulses (up to more than 100). High level of overlap in the Principal Component Analysis made difficult to differentiate sound type clusters. Despite this, two sound types were identifiable: knocks (sounds from 1 to 3 pulses) and long grunts (sounds with more than 29 pulses). Discriminant Analysis carried out on PCA residuals showed that knock had the highest proportion of correct placement (92% of the observations correctly placed) followed by long grunts (80%). All other previously described sound types (intermediate grunt, short grunt and disturbance sounds) could not be separated and presented low levels of correct placement, suggesting that care should be taken when defining these as independent sound types. Finally, acoustic features consistency was found in meagre grunts emitted by different populations during spawning nights; statistical differences could be explained by recording settings and fish conditions. The results of this study provide important information for fostering PAM programs of wild meagre populations, while contributing to the discussion around the definition of fish sound types in vocal fish communities. Studies of this kind, which evaluate both variability and consistency of sound features, are of fundamental importance for maximising PAM efforts in the wild, at both the specific and the community level.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Passive Acoustic Monitoring (PAM) is a non-intrusive and cost-effective method capable of providing high-resolution, long-term information on the status and health of vocal populations and communities. To successfully monitor the same species over wide geographical and temporal scales, it is necessary to characterise the range of sound variability, as well as the consistency of sound features between populations. The meagre ( Argyrosomus regius , Asso 1801) is an interesting case study because recent investigations suggest a wider vocal repertoire than previously described. In this study, meagre vocalizations were recorded and analysed from a variety of settings, ranging from rearing facilities to wild populations to provide a comprehensive characterisation of its vocal repertoire, while investigating the consistency of spawning sound features between populations. All sounds presented a similar acoustic structure in their basic unit ( i . e . the pulse), while an important variability was found in the number of pulses; the meagre can emit sounds made of one single pulse or many pulses (up to more than 100). High level of overlap in the Principal Component Analysis made difficult to differentiate sound type clusters. Despite this, two sound types were identifiable: knocks (sounds from 1 to 3 pulses) and long grunts (sounds with more than 29 pulses). Discriminant Analysis carried out on PCA residuals showed that knock had the highest proportion of correct placement (92% of the observations correctly placed) followed by long grunts (80%). All other previously described sound types (intermediate grunt, short grunt and disturbance sounds) could not be separated and presented low levels of correct placement, suggesting that care should be taken when defining these as independent sound types. Finally, acoustic features consistency was found in meagre grunts emitted by different populations during spawning nights; statistical differences could be explained by recording settings and fish conditions. The results of this study provide important information for fostering PAM programs of wild meagre populations, while contributing to the discussion around the definition of fish sound types in vocal fish communities. Studies of this kind, which evaluate both variability and consistency of sound features, are of fundamental importance for maximising PAM efforts in the wild, at both the specific and the community level. |
Misol, Gerald N; Kokkari, Constantinta; Katharios, Pantelis Complete Genome Sequence of a Jumbo Bacteriophage, vB_pir03, against textitVibrio harveyi Journal Article Microbiology Resource Announcements, 9 (44), pp. e00910–20, /mra/9/44/MRA.00910–20.atom, 2020, ISSN: 2576-098X. @article{misol_complete_2020, title = {Complete Genome Sequence of a Jumbo Bacteriophage, vB_pir03, against textitVibrio harveyi}, author = {Gerald N Misol and Constantinta Kokkari and Pantelis Katharios}, editor = {John J Dennehy}, url = {https://mra.asm.org/content/9/44/e00910-20 https://imbbc.hcmr.gr/wp-content/uploads/2020/12/Katharios-2020-Microbiology-Resource-Announcements.pdf}, doi = {10.1128/MRA.00910-20}, issn = {2576-098X}, year = {2020}, date = {2020-10-01}, urldate = {2020-12-08}, journal = {Microbiology Resource Announcements}, volume = {9}, number = {44}, pages = {e00910--20, /mra/9/44/MRA.00910--20.atom}, abstract = {ABSTRACT Vibrio harveyi is a persistent pathogen responsible for disease outbreaks in aquaculture. We have sequenced the genome of a jumbo Vibrio phage, vB_pir03, isolated in Greece. Here, we present the complete genome of vB_pir03, which consists of 286,284 bp and 336 open reading frames.}, keywords = {}, pubstate = {published}, tppubtype = {article} } ABSTRACT Vibrio harveyi is a persistent pathogen responsible for disease outbreaks in aquaculture. We have sequenced the genome of a jumbo Vibrio phage, vB_pir03, isolated in Greece. Here, we present the complete genome of vB_pir03, which consists of 286,284 bp and 336 open reading frames. |
Fakriadis, Ioannis; Zanatta, Eugenio Maria; Fleck, Renata Pontes Dos Santos; Mateo, Daybet Lorena Sena; Papadaki, Maria; Mylonas, Constantinos C Endocrine regulation of long-term enhancement of spermiation in meagre (Argyrosomus regius) with GnRHa controlled-delivery systems Journal Article General and Comparative Endocrinology, 297 , pp. 113549, 2020, ISSN: 00166480. @article{fakriadis_endocrine_2020, title = {Endocrine regulation of long-term enhancement of spermiation in meagre (Argyrosomus regius) with GnRHa controlled-delivery systems}, author = {Ioannis Fakriadis and Eugenio Maria Zanatta and Renata Pontes Dos Santos Fleck and Daybet Lorena Sena Mateo and Maria Papadaki and Constantinos C Mylonas}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0016648020303026}, doi = {10.1016/j.ygcen.2020.113549}, issn = {00166480}, year = {2020}, date = {2020-10-01}, urldate = {2020-08-06}, journal = {General and Comparative Endocrinology}, volume = {297}, pages = {113549}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Papadaki, Maria; Kaitetzidou, Elisavet; Mylonas, Constantinos C; Sarropoulou, Elena Non-coding RNA Expression Patterns of Two Different Teleost Gonad Maturation Stages Journal Article Marine Biotechnology, 22 (5), pp. 683–695, 2020, ISSN: 1436-2228, 1436-2236. @article{papadaki_non-coding_2020, title = {Non-coding RNA Expression Patterns of Two Different Teleost Gonad Maturation Stages}, author = {Maria Papadaki and Elisavet Kaitetzidou and Constantinos C Mylonas and Elena Sarropoulou}, url = {http://link.springer.com/10.1007/s10126-020-09991-2 https://imbbc.hcmr.gr/wp-content/uploads/2020/10/Papadaki-2020-MBTE-pre-print.pdf}, doi = {10.1007/s10126-020-09991-2}, issn = {1436-2228, 1436-2236}, year = {2020}, date = {2020-10-01}, urldate = {2020-10-05}, journal = {Marine Biotechnology}, volume = {22}, number = {5}, pages = {683--695}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Chatziantoniou, Andromachi; Bakopoulos, Vasileios; Papandroulakis, Nikos; Topouzelis, Konstantinos Detection of biogenic oil film near aquaculture sites seen by Sentinel-2 multispectral images Inproceedings Bostater, Charles R; Neyt, Xavier; Viallefont-Robinet, Françoise (Ed.): Remote Sensing of the Ocean, Sea Ice, Coastal Waters, and Large Water Regions 2020, pp. 4, SPIE, Online Only, United Kingdom, 2020, ISBN: 978-1-5106-3871-6 978-1-5106-3872-3. @inproceedings{chatziantoniou_detection_2020, title = {Detection of biogenic oil film near aquaculture sites seen by Sentinel-2 multispectral images}, author = {Andromachi Chatziantoniou and Vasileios Bakopoulos and Nikos Papandroulakis and Konstantinos Topouzelis}, editor = {Charles R Bostater and Xavier Neyt and Françoise Viallefont-Robinet}, url = {https://www.spiedigitallibrary.org/conference-proceedings-of-spie/11529/2573455/Detection-of-biogenic-oil-film-near-aquaculture-sites-seen-by/10.1117/12.2573455.full}, doi = {10.1117/12.2573455}, isbn = {978-1-5106-3871-6 978-1-5106-3872-3}, year = {2020}, date = {2020-09-01}, urldate = {2020-10-05}, booktitle = {Remote Sensing of the Ocean, Sea Ice, Coastal Waters, and Large Water Regions 2020}, pages = {4}, publisher = {SPIE}, address = {Online Only, United Kingdom}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Henry, Morgane A; Kokou, Fotini; Palenzuela, Oswaldo; Pyrenis, George; Rigos, George Folia Parasitologica, 67 , 2020, ISSN: 00155683, 18036465. @article{henry_experimental_2020, title = {Experimental infection model with the bivalvulid Enteromyxum leei (Myxidiidae) in the sharpsnout seabream, Diplodus puntazzo (Sparidae), and evaluation of the antiparasitic efficacy of a functional diet}, author = {Morgane A Henry and Fotini Kokou and Oswaldo Palenzuela and George Pyrenis and George Rigos}, url = {http://folia.paru.cas.cz/doi/10.14411/fp.2020.024.html}, doi = {10.14411/fp.2020.024}, issn = {00155683, 18036465}, year = {2020}, date = {2020-09-01}, urldate = {2020-12-08}, journal = {Folia Parasitologica}, volume = {67}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Polymenakou, Paraskevi N; Mandalakis, Manolis; Macheras, Michalis; Oulas, Anastasis; Kristoffersen, Jon Bent; Christakis, Christos A; Terzoglou, Vasso; Stavroulaki, Melanthia High genetic diversity and variability of microbial communities in near-surface atmosphere of Crete island, Greece Journal Article Aerobiologia, 36 (3), pp. 341–353, 2020, ISSN: 0393-5965, 1573-3025. @article{polymenakou_high_2020, title = {High genetic diversity and variability of microbial communities in near-surface atmosphere of Crete island, Greece}, author = {Paraskevi N Polymenakou and Manolis Mandalakis and Michalis Macheras and Anastasis Oulas and Jon Bent Kristoffersen and Christos A Christakis and Vasso Terzoglou and Melanthia Stavroulaki}, url = {http://link.springer.com/10.1007/s10453-020-09636-w}, doi = {10.1007/s10453-020-09636-w}, issn = {0393-5965, 1573-3025}, year = {2020}, date = {2020-09-01}, urldate = {2020-08-31}, journal = {Aerobiologia}, volume = {36}, number = {3}, pages = {341--353}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Chatziplis, Dimitrios; Oikonomou, Stavroula; Loukovitis, Dimitrios; Tsiokos, Dimitrios; Samaras, Athanasios; Dimitroglou, Arkadios; Kottaras, Lefteris; Papanna, Kantham; Papaharisis, Leonidas; Tsigenopoulos, Costas; Pavlidis, Michail QTL for Stress and Disease Resistance in European Sea Bass, Dicentrarhus labrax L. Journal Article Animals, 10 (9), pp. 1668, 2020, ISSN: 2076-2615. @article{chatziplis_qtl_2020, title = {QTL for Stress and Disease Resistance in European Sea Bass, Dicentrarhus labrax L.}, author = {Dimitrios Chatziplis and Stavroula Oikonomou and Dimitrios Loukovitis and Dimitrios Tsiokos and Athanasios Samaras and Arkadios Dimitroglou and Lefteris Kottaras and Kantham Papanna and Leonidas Papaharisis and Costas Tsigenopoulos and Michail Pavlidis}, url = {https://www.mdpi.com/2076-2615/10/9/1668}, doi = {10.3390/ani10091668}, issn = {2076-2615}, year = {2020}, date = {2020-09-01}, urldate = {2020-10-05}, journal = {Animals}, volume = {10}, number = {9}, pages = {1668}, abstract = {There is a growing interest in selective breeding in European sea bass (Dicentrarchus labrax), especially regarding family selection based on growth performance. In particular, quantitative trait loci (QTL) identification in sea bass enhances the application of marker-assisted breeding for the genetic improvement of the production traits. The aims of the study were to identify potential QTL affecting stress and immunological indicators, body weight, and mortality after vibriosis injection in sea bass as well as to estimate heritability and genetic/phenotypic correlations for the aforementioned traits. To this end, stress test was performed on 960 offspring and a sub-group of them (420) was selected to explore the mortality after vibrio injection. Selective genotyping was performed in 620 offspring for 35 microsatellite markers and distributed into 6 linkage groups. The length of the genetic linkage map was 283.6 cM and the mean distance between the markers was 8.1 cM. QTL affecting body weight in three different growth periods detected on linkage groups LG1, LG4, LG6, and LG14. A QTL associated with weight in early growth stages (290–306 days post-hatching) was also identified on LG3. QTL analysis confirmed the existence of QTL affecting cortisol levels, on LG3 and LG14. Moreover, new QTL affecting only cortisol and glucose levels were detected on LG1 and LG23. No QTL affecting hormonal or biochemical marks was found on LG4 and LG6. Heritability of cortisol, lysozyme levels, and mortality were high (0.36, 0.55, and 0.38, respectively).}, keywords = {}, pubstate = {published}, tppubtype = {article} } There is a growing interest in selective breeding in European sea bass (Dicentrarchus labrax), especially regarding family selection based on growth performance. In particular, quantitative trait loci (QTL) identification in sea bass enhances the application of marker-assisted breeding for the genetic improvement of the production traits. The aims of the study were to identify potential QTL affecting stress and immunological indicators, body weight, and mortality after vibriosis injection in sea bass as well as to estimate heritability and genetic/phenotypic correlations for the aforementioned traits. To this end, stress test was performed on 960 offspring and a sub-group of them (420) was selected to explore the mortality after vibrio injection. Selective genotyping was performed in 620 offspring for 35 microsatellite markers and distributed into 6 linkage groups. The length of the genetic linkage map was 283.6 cM and the mean distance between the markers was 8.1 cM. QTL affecting body weight in three different growth periods detected on linkage groups LG1, LG4, LG6, and LG14. A QTL associated with weight in early growth stages (290–306 days post-hatching) was also identified on LG3. QTL analysis confirmed the existence of QTL affecting cortisol levels, on LG3 and LG14. Moreover, new QTL affecting only cortisol and glucose levels were detected on LG1 and LG23. No QTL affecting hormonal or biochemical marks was found on LG4 and LG6. Heritability of cortisol, lysozyme levels, and mortality were high (0.36, 0.55, and 0.38, respectively). |
Mastoraki, Maria; Vlahos, Nikolaos; Patsea, Efstathia; Chatzifotis, Stavros; Mente, Eleni; Antonopoulou, Efthimia Aquaculture Research, 51 (9), pp. 3551–3562, 2020, ISSN: 1355-557X, 1365-2109. @article{mastoraki_effect_2020, title = {The effect of insect meal as a feed ingredient on survival, growth, and metabolic and antioxidant response of juvenile prawn textitPalaemon adspersus (Rathke, 1837)}, author = {Maria Mastoraki and Nikolaos Vlahos and Efstathia Patsea and Stavros Chatzifotis and Eleni Mente and Efthimia Antonopoulou}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/are.14692}, doi = {10.1111/are.14692}, issn = {1355-557X, 1365-2109}, year = {2020}, date = {2020-09-01}, urldate = {2020-08-31}, journal = {Aquaculture Research}, volume = {51}, number = {9}, pages = {3551--3562}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Pérez, José A; Papadakis, Ioannis E; Papandroulakis, Nikos; Cruces, Lorenzo; Cotou, Efthimia; Gisbert, Enric; Lorenzo, Antonio; Mylonas, Constantinos C; Rodríguez, Covadonga Aquaculture Nutrition, pp. anu.13128, 2020, ISSN: 1353-5773, 1365-2095. @article{perez_ontogeny_2020, title = {The ontogeny of greater amberjack digestive and antioxidant defence systems under different rearing conditions: A histological and enzymatic approach}, author = {José A Pérez and Ioannis E Papadakis and Nikos Papandroulakis and Lorenzo Cruces and Efthimia Cotou and Enric Gisbert and Antonio Lorenzo and Constantinos C Mylonas and Covadonga Rodríguez}, url = {https://onlinelibrary.wiley.com/doi/10.1111/anu.13128}, doi = {10.1111/anu.13128}, issn = {1353-5773, 1365-2095}, year = {2020}, date = {2020-09-01}, urldate = {2020-10-05}, journal = {Aquaculture Nutrition}, pages = {anu.13128}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Paspalakis, Stavros; Moirogiorgou, Konstantia; Papandroulakis, Nikos; Giakos, George; Zervakis, Michalis Automated fish cage net inspection using image processing techniques Journal Article IET Image Processing, 14 (10), pp. 2028–2034, 2020, ISSN: 1751-9659, 1751-9667. @article{paspalakis_automated_2020, title = {Automated fish cage net inspection using image processing techniques}, author = {Stavros Paspalakis and Konstantia Moirogiorgou and Nikos Papandroulakis and George Giakos and Michalis Zervakis}, url = {https://digital-library.theiet.org/content/journals/10.1049/iet-ipr.2019.1667}, doi = {10.1049/iet-ipr.2019.1667}, issn = {1751-9659, 1751-9667}, year = {2020}, date = {2020-08-01}, urldate = {2020-08-06}, journal = {IET Image Processing}, volume = {14}, number = {10}, pages = {2028--2034}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Mokos, Melita; Cheimonopoulou, Maria Th.; Koulouri, Panayota; Previati, Monica; Realdon, Giulia; Santoro, Francesca; Mogias, Athanasios; Boubonari, Theodora; Gazo, Manel; Satta, Alessio; Ioakeimidis, Christos; Tojeiro, Alba; Chicote, Carla A; Papathanassiou, Martha; Kevrekidis, Theodoros Mediterranean Sea Literacy: When Ocean Literacy becomes region- specific Journal Article Mediterranean Marine Science, 2020, ISSN: 1791-6763, 1108-393X. @article{mokos_mediterranean_2020, title = {Mediterranean Sea Literacy: When Ocean Literacy becomes region- specific}, author = {Melita Mokos and Maria Th. Cheimonopoulou and Panayota Koulouri and Monica Previati and Giulia Realdon and Francesca Santoro and Athanasios Mogias and Theodora Boubonari and Manel Gazo and Alessio Satta and Christos Ioakeimidis and Alba Tojeiro and Carla A Chicote and Martha Papathanassiou and Theodoros Kevrekidis}, url = {https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/23400}, doi = {10.12681/mms.23400}, issn = {1791-6763, 1108-393X}, year = {2020}, date = {2020-08-01}, urldate = {2020-10-05}, journal = {Mediterranean Marine Science}, abstract = {Ocean Literacy (OL) has been defined as an understanding of the ocean’s influence on people and their influence on the ocean. The OL movement was born in the US and its framework consisted of seven essential principles and 45 fundamental concepts; it is now largely accepted worldwide for use in both formal (schools and universities) and non-formal (research institutes, aquaria, museums, etc.) education settings. Based on this framework, marine scientists and educators developed the “Mediterranean Sea Literacy” (MSL) guide adapted to the specificities of the Mediterranean region, presented here. The MSL principles (7) and concepts (43), serving as guidance for research, education, informed decision-making, and improved citizens’ lifestyles, aim to contribute to environmental protection, conservation, and restoration of the Mediterranean Sea as well as to help to achieve a blue innovative and sustainable economy.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Ocean Literacy (OL) has been defined as an understanding of the ocean’s influence on people and their influence on the ocean. The OL movement was born in the US and its framework consisted of seven essential principles and 45 fundamental concepts; it is now largely accepted worldwide for use in both formal (schools and universities) and non-formal (research institutes, aquaria, museums, etc.) education settings. Based on this framework, marine scientists and educators developed the “Mediterranean Sea Literacy” (MSL) guide adapted to the specificities of the Mediterranean region, presented here. The MSL principles (7) and concepts (43), serving as guidance for research, education, informed decision-making, and improved citizens’ lifestyles, aim to contribute to environmental protection, conservation, and restoration of the Mediterranean Sea as well as to help to achieve a blue innovative and sustainable economy. |
Tsiola, Anastasia; Michoud, Grégoire; Fodelianakis, Stilianos; Karakassis, Ioannis; Kotoulas, Georgios; Pavlidou, Alexandra; Pavloudi, Christina; Pitta, Paraskevi; Simboura, Nomiki; Daffonchio, Daniele; Tsapakis, Manolis Viral Metagenomic Content Reflects Seawater Ecological Quality in the Coastal Zone Journal Article Viruses, 12 (8), pp. 806, 2020. @article{tsiola_viral_2020, title = {Viral Metagenomic Content Reflects Seawater Ecological Quality in the Coastal Zone}, author = {Anastasia Tsiola and Grégoire Michoud and Stilianos Fodelianakis and Ioannis Karakassis and Georgios Kotoulas and Alexandra Pavlidou and Christina Pavloudi and Paraskevi Pitta and Nomiki Simboura and Daniele Daffonchio and Manolis Tsapakis}, url = {https://www.mdpi.com/1999-4915/12/8/806}, doi = {10.3390/v12080806}, year = {2020}, date = {2020-08-01}, urldate = {2020-08-06}, journal = {Viruses}, volume = {12}, number = {8}, pages = {806}, abstract = {Viruses interfere with their host’s metabolism through the expression of auxiliary metabolic genes (AMGs) that, until now, are mostly studied under large physicochemical gradients. Here, we focus on coastal marine ecosystems and we sequence the viral metagenome (virome) of samples with discrete levels of human-driven disturbances. We aim to describe the relevance of viromics with respect to ecological quality status, defined by the classic seawater trophic index (TRIX). Neither viral (family level) nor bacterial (family level, based on 16S rRNA sequencing) community structure correlated with TRIX. AMGs involved in the Calvin and tricarboxylic acid cycles were found at stations with poor ecological quality, supporting viral lysis by modifying the host’s energy supply. AMGs involved in “non-traditional” energy-production pathways (3HP, sulfur oxidation) were found irrespective of ecological quality, highlighting the importance of recognizing the prevalent metabolic paths and their intermediate byproducts. Various AMGs explained the variability between stations with poor vs. good ecological quality. Our study confirms the pivotal role of the virome content in ecosystem functioning, acting as a “pool” of available functions that may be transferred to the hosts. Further, it suggests that AMGs could be used as an ultra-sensitive metric of energy-production pathways with relevance in the vulnerable coastal zone and its ecological quality.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Viruses interfere with their host’s metabolism through the expression of auxiliary metabolic genes (AMGs) that, until now, are mostly studied under large physicochemical gradients. Here, we focus on coastal marine ecosystems and we sequence the viral metagenome (virome) of samples with discrete levels of human-driven disturbances. We aim to describe the relevance of viromics with respect to ecological quality status, defined by the classic seawater trophic index (TRIX). Neither viral (family level) nor bacterial (family level, based on 16S rRNA sequencing) community structure correlated with TRIX. AMGs involved in the Calvin and tricarboxylic acid cycles were found at stations with poor ecological quality, supporting viral lysis by modifying the host’s energy supply. AMGs involved in “non-traditional” energy-production pathways (3HP, sulfur oxidation) were found irrespective of ecological quality, highlighting the importance of recognizing the prevalent metabolic paths and their intermediate byproducts. Various AMGs explained the variability between stations with poor vs. good ecological quality. Our study confirms the pivotal role of the virome content in ecosystem functioning, acting as a “pool” of available functions that may be transferred to the hosts. Further, it suggests that AMGs could be used as an ultra-sensitive metric of energy-production pathways with relevance in the vulnerable coastal zone and its ecological quality. |
Correia, Miguel; Paulo, Diogo; Samara, Elina; Koulouri, Panayota; Mentogiannis, Vasilis; Dounas, Costas Journal of Fish Biology, 97 (1), pp. 314–317, 2020, ISSN: 0022-1112, 1095-8649. @article{correia_field_2020, title = {Field studies of seahorse population density, structure and habitat use in a semi‐closed north‐eastern Mediterranean marine area (Stratoni, North Aegean Sea)}, author = {Miguel Correia and Diogo Paulo and Elina Samara and Panayota Koulouri and Vasilis Mentogiannis and Costas Dounas}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/jfb.14371}, doi = {10.1111/jfb.14371}, issn = {0022-1112, 1095-8649}, year = {2020}, date = {2020-07-01}, urldate = {2020-08-31}, journal = {Journal of Fish Biology}, volume = {97}, number = {1}, pages = {314--317}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Rigos, George; Katharios, Pantelis; Kogiannou, Dimitra; Cascarano, Chiara M Infectious diseases and treatment solutions of farmed greater amberjack Seriola dumerili with particular emphasis in Mediterranean region Journal Article Reviews in Aquaculture, pp. raq.12476, 2020, ISSN: 1753-5123, 1753-5131. @article{rigos_infectious_2020, title = {Infectious diseases and treatment solutions of farmed greater amberjack Seriola dumerili with particular emphasis in Mediterranean region}, author = {George Rigos and Pantelis Katharios and Dimitra Kogiannou and Chiara M Cascarano}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/raq.12476}, doi = {10.1111/raq.12476}, issn = {1753-5123, 1753-5131}, year = {2020}, date = {2020-07-01}, urldate = {2020-09-02}, journal = {Reviews in Aquaculture}, pages = {raq.12476}, abstract = {Abstract Greater amberjack (Seriola dumerili) is a very promising candidate for the diversification of the Mediterranean aquaculture due to its high demand, excellent flesh quality and high market prices. Its production expansion has, however, failed so far, due to several bottlenecks mainly related to pathology. This review addresses the major pathogens, which hinder the culture of greater amberjack with special focus in the Mediterranean region, and highlights possible treatment solutions. Among the important recorded pathogens of caged greater amberjack in the Mediterranean, the gill monogenean Zeuxapta seriolae seems to be the most problematic, causing significant losses. While formalin immersions are inefficient to combat this parasite, baths with hydrogen peroxide are extremely effective and praziquantel administration could be a more practical in-feed treatment solution. The digenean blood flukes, Paradeontacylix spp., also account for important losses in greater amberjack farms in the same region. Dietary administration of praziquantel constitutes an effective therapeutic measure against those infections. Vibrio harveyi is also a bacterial pathogen severely affecting fish maintained both in land-based facilities and in cages, whereas Epitheliocystis is a disease reported frequently that can be fatal when it occurs at early stages. Skin flukes such as Benedenia seriolae and Neobenedenia girellae as well as other parasites bacteria and viruses mentioned herein, which have caused substantial losses in Asian enterprises, but have not been identified yet in greater amberjack farmed in the Mediterranean, should be considered as potential threats.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Greater amberjack (Seriola dumerili) is a very promising candidate for the diversification of the Mediterranean aquaculture due to its high demand, excellent flesh quality and high market prices. Its production expansion has, however, failed so far, due to several bottlenecks mainly related to pathology. This review addresses the major pathogens, which hinder the culture of greater amberjack with special focus in the Mediterranean region, and highlights possible treatment solutions. Among the important recorded pathogens of caged greater amberjack in the Mediterranean, the gill monogenean Zeuxapta seriolae seems to be the most problematic, causing significant losses. While formalin immersions are inefficient to combat this parasite, baths with hydrogen peroxide are extremely effective and praziquantel administration could be a more practical in-feed treatment solution. The digenean blood flukes, Paradeontacylix spp., also account for important losses in greater amberjack farms in the same region. Dietary administration of praziquantel constitutes an effective therapeutic measure against those infections. Vibrio harveyi is also a bacterial pathogen severely affecting fish maintained both in land-based facilities and in cages, whereas Epitheliocystis is a disease reported frequently that can be fatal when it occurs at early stages. Skin flukes such as Benedenia seriolae and Neobenedenia girellae as well as other parasites bacteria and viruses mentioned herein, which have caused substantial losses in Asian enterprises, but have not been identified yet in greater amberjack farmed in the Mediterranean, should be considered as potential threats. |
Zotou, Maria; Gkrantounis, Pavlos; Karadimou, Elpida; Tsirintanis, Konstantinos; Sini, Maria; Poursanidis, Dimitris; Azzolin, Marta; Dailianis, Thanos; Kytinou, Eleni; Issaris, Yiannis; Gerakaris, Vasilis; Salomidi, Maria; Lardi, Poly; Ramfos, Alexis; Akrivos, Vasilis; Spinos, Efthimios; Dimitriadis, Charalampos; Papageorgiou, Dimitris; Lattos, Athanasios; Giantsis, Ioannis A; Michaelidis, Basile; Vassilopoulou, Vasiliki; Miliou, Anastasia; Katsanevakis, Stelios Pinna nobilis in the Greek seas (NE Mediterranean): on the brink of extinction? Journal Article Mediterranean Marine Science, 2020, ISSN: 1791-6763, 1108-393X. @article{zotou_pinna_2020, title = {Pinna nobilis in the Greek seas (NE Mediterranean): on the brink of extinction?}, author = {Maria Zotou and Pavlos Gkrantounis and Elpida Karadimou and Konstantinos Tsirintanis and Maria Sini and Dimitris Poursanidis and Marta Azzolin and Thanos Dailianis and Eleni Kytinou and Yiannis Issaris and Vasilis Gerakaris and Maria Salomidi and Poly Lardi and Alexis Ramfos and Vasilis Akrivos and Efthimios Spinos and Charalampos Dimitriadis and Dimitris Papageorgiou and Athanasios Lattos and Ioannis A Giantsis and Basile Michaelidis and Vasiliki Vassilopoulou and Anastasia Miliou and Stelios Katsanevakis}, url = {https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/23777}, doi = {10.12681/mms.23777}, issn = {1791-6763, 1108-393X}, year = {2020}, date = {2020-07-01}, urldate = {2020-10-05}, journal = {Mediterranean Marine Science}, abstract = {The Mediterranean endemic fan mussel Pinna nobilis is suffering an ongoing basin-scale mass mortality event (MME) since 2016. As most Mediterranean populations have collapsed, the species has been declared as Critically Endangered in the IUCN Red List of threatened species. In an effort to track the progress of the MME and provide updated information on the status of the species in the Greek seas, we compiled data collected through dedicated surveys and opportunistic assessments during 2019 and 2020. A total of 14589 fan mussel individuals, of which 81.1% dead, were recorded in 258 site surveys. Of the remaining 2762 live individuals, 256 were juveniles. Two marine areas which still sustain living populations were identified, namely Kalloni Gulf (Lesvos Island), and Laganas Bay (Zakynthos Island). The inner part of Kalloni Gulf appears to maintain the largest surviving population of the species in the eastern Mediterranean, with an abundance estimate of 684000 individuals (95% confidence interval: 322000-1453000). Solitary, potentially resistant, scattered individuals were recorded in several sites. Other previously abundant populations that had been assessed in the past, specifically those of Lake Vouliagmeni (Korinthiakos Gulf), Souda Bay (Crete) and Gera Gulf (Lesvos Island), and which collectively summed up to textasciitilde350,000 individuals, have now been wiped out. Our results document the collapse of most P. nobilis populations throughout the Greek seas. The MME has substantially progressed between early 2019 and mid-2020, as indicated by the increase of mortality at sites consecutively monitored multiple times. This work highlights the urgent need for continuous monitoring of surviving populations and calls for the immediate implementation of an effective protection and management strategy that will ensure the persistence of surviving individuals and the production of resistant offspring.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The Mediterranean endemic fan mussel Pinna nobilis is suffering an ongoing basin-scale mass mortality event (MME) since 2016. As most Mediterranean populations have collapsed, the species has been declared as Critically Endangered in the IUCN Red List of threatened species. In an effort to track the progress of the MME and provide updated information on the status of the species in the Greek seas, we compiled data collected through dedicated surveys and opportunistic assessments during 2019 and 2020. A total of 14589 fan mussel individuals, of which 81.1% dead, were recorded in 258 site surveys. Of the remaining 2762 live individuals, 256 were juveniles. Two marine areas which still sustain living populations were identified, namely Kalloni Gulf (Lesvos Island), and Laganas Bay (Zakynthos Island). The inner part of Kalloni Gulf appears to maintain the largest surviving population of the species in the eastern Mediterranean, with an abundance estimate of 684000 individuals (95% confidence interval: 322000-1453000). Solitary, potentially resistant, scattered individuals were recorded in several sites. Other previously abundant populations that had been assessed in the past, specifically those of Lake Vouliagmeni (Korinthiakos Gulf), Souda Bay (Crete) and Gera Gulf (Lesvos Island), and which collectively summed up to textasciitilde350,000 individuals, have now been wiped out. Our results document the collapse of most P. nobilis populations throughout the Greek seas. The MME has substantially progressed between early 2019 and mid-2020, as indicated by the increase of mortality at sites consecutively monitored multiple times. This work highlights the urgent need for continuous monitoring of surviving populations and calls for the immediate implementation of an effective protection and management strategy that will ensure the persistence of surviving individuals and the production of resistant offspring. |
Santi, I; Kasapidis, P; Psarra, S; Assimakopoulou, G; Pavlidou, A; Protopapa, M; Tsiola, A; Zeri, C; Pitta, P Composition and distribution patterns of eukaryotic microbial plankton in the ultra-oligotrophic Eastern Mediterranean Sea Journal Article Aquatic Microbial Ecology, 84 , pp. 155–173, 2020, ISSN: 0948-3055, (Publisher: Inter-Research Science Center). @article{santi_composition_2020, title = {Composition and distribution patterns of eukaryotic microbial plankton in the ultra-oligotrophic Eastern Mediterranean Sea}, author = {I Santi and P Kasapidis and S Psarra and G Assimakopoulou and A Pavlidou and M Protopapa and A Tsiola and C Zeri and P Pitta}, url = {https://www.int-res.com/abstracts/ame/v84/p155-173/}, doi = {10.3354/ame01933}, issn = {0948-3055}, year = {2020}, date = {2020-06-01}, journal = {Aquatic Microbial Ecology}, volume = {84}, pages = {155--173}, abstract = {Marine microbial eukaryotes play crucial roles in water-column ecosystems; however, there are regional gaps in the investigation of natural microbial eukaryote communities, and uncertainties concerning their distribution persevere. This study combined 18S rRNA metabarcoding, biomass measurements and statistical analyses of multiple environmental variables to examine the distribution of planktonic microbial eukaryotes at different sites and water layers in the ultra-oligotrophic Eastern Mediterranean Sea (Western Levantine Basin). Our results showed that microbial eukaryotic communities were structured by depth. In surface waters, different sites shared high percentages of molecular operational taxonomic units (MOTUs), but this was not the case for deep-sea communities (≥1000 m). Plankton biomass was significantly different among sites, implying that communities of a similar composition may not support the same activity or population size. The deep-sea communities showed high percentages of unassigned MOTUs, highlighting the sparsity of the existing information on deep-sea plankton eukaryotes. Water temperature and dissolved organic matter significantly affected community distribution. Micro-eukaryotic distribution was additionally affected by the nitrogen to phosphorus ratio and viral abundance, while nano- and pico-communities were affected by zooplankton. The present study explores microbial plankton eukaryotes in their natural oligotrophic environment and highlights that, even within restricted oceanic areas, marine plankton may follow distribution patterns that are largely controlled by environmental variables.}, note = {Publisher: Inter-Research Science Center}, keywords = {}, pubstate = {published}, tppubtype = {article} } Marine microbial eukaryotes play crucial roles in water-column ecosystems; however, there are regional gaps in the investigation of natural microbial eukaryote communities, and uncertainties concerning their distribution persevere. This study combined 18S rRNA metabarcoding, biomass measurements and statistical analyses of multiple environmental variables to examine the distribution of planktonic microbial eukaryotes at different sites and water layers in the ultra-oligotrophic Eastern Mediterranean Sea (Western Levantine Basin). Our results showed that microbial eukaryotic communities were structured by depth. In surface waters, different sites shared high percentages of molecular operational taxonomic units (MOTUs), but this was not the case for deep-sea communities (≥1000 m). Plankton biomass was significantly different among sites, implying that communities of a similar composition may not support the same activity or population size. The deep-sea communities showed high percentages of unassigned MOTUs, highlighting the sparsity of the existing information on deep-sea plankton eukaryotes. Water temperature and dissolved organic matter significantly affected community distribution. Micro-eukaryotic distribution was additionally affected by the nitrogen to phosphorus ratio and viral abundance, while nano- and pico-communities were affected by zooplankton. The present study explores microbial plankton eukaryotes in their natural oligotrophic environment and highlights that, even within restricted oceanic areas, marine plankton may follow distribution patterns that are largely controlled by environmental variables. |
Çinar, Melih Ertan; Féral, Jean‐Pierre; Arvanitidis, Christos; David, Romain; Taşkin, Ergün; Sini, Maria; Dailianis, Thanos; Doğan, Alper; Gerovasileiou, Vasilis; Evcen, Alper; Chenuil, Anne; Dağli, Ertan; Aysel, Veysel; Issaris, Yannis; Bakir, Kerem; Nalmpantı, Melina; Sartoretto, Stephane; Salomidi, Maria; Sapouna, Anastasia; Açik, Sermin; Dimitriadis, Charalampos; Koutsoubas, Drosos; Katağan, Tuncer; Öztürk, Bilal; Koçak, Ferah; Erdogan‐Dereli, Deniz; Önen, Senem; Özgen, Özge; Türkçü, Neslihan; Kirkim, Fevzi; Önen, Mesut Coralligenous assemblages along their geographical distribution: Testing of concepts and implications for management Journal Article Aquatic Conservation: Marine and Freshwater Ecosystems, 30 , pp. 1578–1594, 2020, ISSN: 1052-7613, 1099-0755. @article{cinar_coralligenous_2020, title = {Coralligenous assemblages along their geographical distribution: Testing of concepts and implications for management}, author = {Melih Ertan Çinar and Jean‐Pierre Féral and Christos Arvanitidis and Romain David and Ergün Taşkin and Maria Sini and Thanos Dailianis and Alper Doğan and Vasilis Gerovasileiou and Alper Evcen and Anne Chenuil and Ertan Dağli and Veysel Aysel and Yannis Issaris and Kerem Bakir and Melina Nalmpantı and Stephane Sartoretto and Maria Salomidi and Anastasia Sapouna and Sermin Açik and Charalampos Dimitriadis and Drosos Koutsoubas and Tuncer Katağan and Bilal Öztürk and Ferah Koçak and Deniz Erdogan‐Dereli and Senem Önen and Özge Özgen and Neslihan Türkçü and Fevzi Kirkim and Mesut Önen}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/aqc.3365}, doi = {10.1002/aqc.3365}, issn = {1052-7613, 1099-0755}, year = {2020}, date = {2020-06-01}, urldate = {2020-08-06}, journal = {Aquatic Conservation: Marine and Freshwater Ecosystems}, volume = {30}, pages = {1578--1594}, abstract = {1. The coralligenous habitat was studied at the large Mediterranean scale, by applying a standardized, non‐destructive photo‐sampling protocol, developed in the framework of the CIGESMED project. 2. The results provided evidence to support the following statements: (a) the assemblage pattern is not homogeneously distributed across the four Mediterranean ecoregions studied (biotic gradients hypothesis); and (b) the assemblage pattern does not change significantly when the information is aggregated to higher taxonomic levels (taxonomic sufficiency hypothesis). 3. Surrogate taxonomic categories higher than species, such as genus and family, can be used to reveal the multivariate pattern of the coralligenous assemblages. 4. Although preliminary at the pan‐Mediterranean scale, these outcomes set the scene for future comparisons as more data sets become available but also for comparisons between taxonomic and functional patterns.}, keywords = {}, pubstate = {published}, tppubtype = {article} } 1. The coralligenous habitat was studied at the large Mediterranean scale, by applying a standardized, non‐destructive photo‐sampling protocol, developed in the framework of the CIGESMED project. 2. The results provided evidence to support the following statements: (a) the assemblage pattern is not homogeneously distributed across the four Mediterranean ecoregions studied (biotic gradients hypothesis); and (b) the assemblage pattern does not change significantly when the information is aggregated to higher taxonomic levels (taxonomic sufficiency hypothesis). 3. Surrogate taxonomic categories higher than species, such as genus and family, can be used to reveal the multivariate pattern of the coralligenous assemblages. 4. Although preliminary at the pan‐Mediterranean scale, these outcomes set the scene for future comparisons as more data sets become available but also for comparisons between taxonomic and functional patterns. |
Antonopoulou, Efthimia; Chatzigiannidou, Ioanna; Feidantsis, Konstantinos; Kounna, Christiana; Chatzifotis, Stavros Effect of water temperature on cellular stress responses in meagre (Argyrosomus regius) Journal Article Fish Physiology and Biochemistry, 46 (3), pp. 1075–1091, 2020, ISSN: 0920-1742, 1573-5168. @article{antonopoulou_effect_2020, title = {Effect of water temperature on cellular stress responses in meagre (Argyrosomus regius)}, author = {Efthimia Antonopoulou and Ioanna Chatzigiannidou and Konstantinos Feidantsis and Christiana Kounna and Stavros Chatzifotis}, url = {http://link.springer.com/10.1007/s10695-020-00773-0}, doi = {10.1007/s10695-020-00773-0}, issn = {0920-1742, 1573-5168}, year = {2020}, date = {2020-06-01}, urldate = {2020-08-31}, journal = {Fish Physiology and Biochemistry}, volume = {46}, number = {3}, pages = {1075--1091}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Alexi, Niki; Luca, Alexandru; Nanou, Evangelia; Byrne, Derek V; Grigorakis, Kriton Aquaculture Research, 51 (6), pp. 2443–2455, 2020, ISSN: 1355-557X, 1365-2109. @article{alexi_investigation_2020, title = {Investigation of the proximate composition, lipid quality, volatile and sensory profiles of wild vs. reared Greater amberjack ( textitSeriola dumerili , Risso)}, author = {Niki Alexi and Alexandru Luca and Evangelia Nanou and Derek V Byrne and Kriton Grigorakis}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/are.14588}, doi = {10.1111/are.14588}, issn = {1355-557X, 1365-2109}, year = {2020}, date = {2020-06-01}, urldate = {2020-10-20}, journal = {Aquaculture Research}, volume = {51}, number = {6}, pages = {2443--2455}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Gastineau, Romain; Lemieux, Claude; Turmel, Monique; Grypioti, Emilia; Verret, Frédéric; Makris, Antonios; Argiriou, Anagnostis; Kafetzopoulos, Dimitris; Stratidaki, Irina; Carrier, Gregory; Jacquette, Boris; Mouget, Jean-Luc Two new bacilladnaviruses associated with the diatom Haslea ostrearia Journal Article European Journal of Phycology, pp. 1–10, 2020, ISSN: 0967-0262, 1469-4433. @article{gastineau_two_2020, title = {Two new bacilladnaviruses associated with the diatom Haslea ostrearia}, author = {Romain Gastineau and Claude Lemieux and Monique Turmel and Emilia Grypioti and Frédéric Verret and Antonios Makris and Anagnostis Argiriou and Dimitris Kafetzopoulos and Irina Stratidaki and Gregory Carrier and Boris Jacquette and Jean-Luc Mouget}, url = {https://www.tandfonline.com/doi/full/10.1080/09670262.2020.1748723}, doi = {10.1080/09670262.2020.1748723}, issn = {0967-0262, 1469-4433}, year = {2020}, date = {2020-06-01}, urldate = {2020-08-18}, journal = {European Journal of Phycology}, pages = {1--10}, abstract = {Haslea ostrearia (Gaillon) Simonsen is famous among diatoms for producing a blue pigment. Genome sequencing of different strains of H. ostrearia led to the discovery of the complete genomes of two new bacilladnaviruses, HOV-148 and HOV-235. The DNA sequences of these viruses were identified in H. ostrearia strains that had been maintained for several years in a culture collection. Some of these strains are the products of in vitro episodes of auxosporulation and thus have never been in contact with their natural biotope. At 4567 and 4538 bp, the HOV-148 and HOV-235 genomes are shorter than those of most diatom-infecting viruses known to date. They each contain four open reading frames and display highest similarities with sequences from viruses found in a gastropod from New Zealand, a proximity confirmed by phylogenetic analysis. HOV-148 and HOV-235 were identified in three clones of H. ostrearia that were collected in oyster ponds from distinct locations in the Bay of Bourgneuf (France). Given that our search for similar viral sequences in genomic data derived from other sub-populations or species of the genus Haslea throughout the world was unsuccessful, we hypothesize that these viruses could have a link with the peculiar biotope of the oyster ponds.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Haslea ostrearia (Gaillon) Simonsen is famous among diatoms for producing a blue pigment. Genome sequencing of different strains of H. ostrearia led to the discovery of the complete genomes of two new bacilladnaviruses, HOV-148 and HOV-235. The DNA sequences of these viruses were identified in H. ostrearia strains that had been maintained for several years in a culture collection. Some of these strains are the products of in vitro episodes of auxosporulation and thus have never been in contact with their natural biotope. At 4567 and 4538 bp, the HOV-148 and HOV-235 genomes are shorter than those of most diatom-infecting viruses known to date. They each contain four open reading frames and display highest similarities with sequences from viruses found in a gastropod from New Zealand, a proximity confirmed by phylogenetic analysis. HOV-148 and HOV-235 were identified in three clones of H. ostrearia that were collected in oyster ponds from distinct locations in the Bay of Bourgneuf (France). Given that our search for similar viral sequences in genomic data derived from other sub-populations or species of the genus Haslea throughout the world was unsuccessful, we hypothesize that these viruses could have a link with the peculiar biotope of the oyster ponds. |
Bounas, Anastasios; Keroglidou, Maria; Toli, Elisavet‐Aspasia; Chousidis, Ieremias; Tsaparis, Dimitris; Leonardos, Ioannis; Sotiropoulos, Konstantinos Constrained by aliens, shifting landscape, or poor water quality? Factors affecting the persistence of amphibians in an urban pond network Journal Article Aquatic Conservation: Marine and Freshwater Ecosystems, 30 (5), pp. 1037–1049, 2020, ISSN: 1052-7613, 1099-0755. @article{bounas_constrained_2020, title = {Constrained by aliens, shifting landscape, or poor water quality? Factors affecting the persistence of amphibians in an urban pond network}, author = {Anastasios Bounas and Maria Keroglidou and Elisavet‐Aspasia Toli and Ieremias Chousidis and Dimitris Tsaparis and Ioannis Leonardos and Konstantinos Sotiropoulos}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/aqc.3309}, doi = {10.1002/aqc.3309}, issn = {1052-7613, 1099-0755}, year = {2020}, date = {2020-05-01}, urldate = {2020-08-26}, journal = {Aquatic Conservation: Marine and Freshwater Ecosystems}, volume = {30}, number = {5}, pages = {1037--1049}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Tsertou, M I; Chatzifotis, S; Fontanillas, R; Cotou, E; Fountoulaki, E; Antonopoulou, E; Katharios, P Aquaculture, 521 , pp. 735052, 2020, ISSN: 00448486. @article{tsertou_effect_2020, title = {The effect of dietary vitamin D3, minerals (Ca, P) and plant-protein sources in the development of systemic granulomatosis in meagre (Argyrosomus regius, Asso, 1801)}, author = {M I Tsertou and S Chatzifotis and R Fontanillas and E Cotou and E Fountoulaki and E Antonopoulou and P Katharios}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848619326626}, doi = {10.1016/j.aquaculture.2020.735052}, issn = {00448486}, year = {2020}, date = {2020-05-01}, urldate = {2020-08-31}, journal = {Aquaculture}, volume = {521}, pages = {735052}, abstract = {Systemic Granulomatosis (SG) affects the majority of cultured meagre (Argyrosomus regius). This pathological condition, whose aetiology is still not known, is identified by multiple granulomas in a variety of organs that become calcified and necrotic with time. The available evidence supports the hypothesis that SG is a metabolic disorder associated with nutritional imbalances, and the present study examined the role of dietary vitamin D3, minerals (Ca, P) and plant-protein sources in the development of the disease. The use of plant-protein in the diet formulation exacerbated the progression of SG, while vitamin D3 did not prevent its occurrence. On the other hand, the severity of SG was improved by increasing the P content in the feed. However, the exact irritant that causes this inflammatory response in meagre is still unknown and more research needs to examine other nutritional factors.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Systemic Granulomatosis (SG) affects the majority of cultured meagre (Argyrosomus regius). This pathological condition, whose aetiology is still not known, is identified by multiple granulomas in a variety of organs that become calcified and necrotic with time. The available evidence supports the hypothesis that SG is a metabolic disorder associated with nutritional imbalances, and the present study examined the role of dietary vitamin D3, minerals (Ca, P) and plant-protein sources in the development of the disease. The use of plant-protein in the diet formulation exacerbated the progression of SG, while vitamin D3 did not prevent its occurrence. On the other hand, the severity of SG was improved by increasing the P content in the feed. However, the exact irritant that causes this inflammatory response in meagre is still unknown and more research needs to examine other nutritional factors. |
Katsanevakis, Stelios; Tsirintanis, Konstantinos; Sini, Maria; Gerovasileiou, Vasilis; Koukourouvli, Nikoletta Aliens in the Aegean – a sea under siege (ALAS) Journal Article Research Ideas and Outcomes, 6 , pp. e53057, 2020, ISSN: 2367-7163. @article{katsanevakis_aliens_2020, title = {Aliens in the Aegean – a sea under siege (ALAS)}, author = {Stelios Katsanevakis and Konstantinos Tsirintanis and Maria Sini and Vasilis Gerovasileiou and Nikoletta Koukourouvli}, url = {https://riojournal.com/article/53057/}, doi = {10.3897/rio.6.e53057}, issn = {2367-7163}, year = {2020}, date = {2020-04-01}, urldate = {2020-08-21}, journal = {Research Ideas and Outcomes}, volume = {6}, pages = {e53057}, abstract = {ALAS aims to fill knowledge gaps on the impacts of marine alien species in the Aegean Sea, and support marine managers and policy makers in prioritizing mitigation actions. The project will focus on under-studied alien-native interactions, priority and vulnerable habitats (such as shallow forests of canopy algae and underwater caves), and apply a multitude of approaches. It will apply a standardized, quantitative method for mapping Cumulative IMpacts of invasive Alien species on marine ecosystems (CIMPAL), according to which cumulative impact scores are estimated on the basis of the distributions of invasive species and ecosystems, and both the reported magnitude of ecological impacts and the strength of such evidence. Towards that direction, ALAS will improve our knowledge base and compile the needed information to estimate CIMPAL by (1) conducting a series of field experiments and surveys to investigate the impacts of selected invasive alien species on marine habitats, (2) producing high-resolution habitat maps in the coastal zone, refining the results of previous research efforts through fieldwork, remote sensing and satellite imaging, (3) producing species distribution models for all invasive species, based on extensive underwater surveys for the collection of new data and integrating all existing information. ALAS will incorporate skills and analyses in novel ways and provide high-resolution results at a large scale; couple classic and novel tools and follow a trans-disciplinary approach, combining knowledge from the fields of invasion biology, conservation biology, biogeography, fisheries science, marine ecology, remote sensing, statistical modelling; conduct for the first time in the Aegean Sea a comprehensive, high-resolution analysis of cumulative impacts of invasive alien species; and report results in formats appropriate for decision-makers and society, thus transferring research-based knowledge to inform and influence policy decisions.}, keywords = {}, pubstate = {published}, tppubtype = {article} } ALAS aims to fill knowledge gaps on the impacts of marine alien species in the Aegean Sea, and support marine managers and policy makers in prioritizing mitigation actions. The project will focus on under-studied alien-native interactions, priority and vulnerable habitats (such as shallow forests of canopy algae and underwater caves), and apply a multitude of approaches. It will apply a standardized, quantitative method for mapping Cumulative IMpacts of invasive Alien species on marine ecosystems (CIMPAL), according to which cumulative impact scores are estimated on the basis of the distributions of invasive species and ecosystems, and both the reported magnitude of ecological impacts and the strength of such evidence. Towards that direction, ALAS will improve our knowledge base and compile the needed information to estimate CIMPAL by (1) conducting a series of field experiments and surveys to investigate the impacts of selected invasive alien species on marine habitats, (2) producing high-resolution habitat maps in the coastal zone, refining the results of previous research efforts through fieldwork, remote sensing and satellite imaging, (3) producing species distribution models for all invasive species, based on extensive underwater surveys for the collection of new data and integrating all existing information. ALAS will incorporate skills and analyses in novel ways and provide high-resolution results at a large scale; couple classic and novel tools and follow a trans-disciplinary approach, combining knowledge from the fields of invasion biology, conservation biology, biogeography, fisheries science, marine ecology, remote sensing, statistical modelling; conduct for the first time in the Aegean Sea a comprehensive, high-resolution analysis of cumulative impacts of invasive alien species; and report results in formats appropriate for decision-makers and society, thus transferring research-based knowledge to inform and influence policy decisions. |
Trichas, Apostolos; Smirli, Maria; Papadopoulou, Anna; Anastasiou, Ioannis; Keskin, Bekir; Poulakakis, Nikos Zoological Journal of the Linnean Society, pp. zlaa022, 2020, ISSN: 0024-4082, 1096-3642. @article{trichas_dispersal_2020, title = {Dispersal versus vicariance in the Aegean: combining molecular and morphological phylogenies of eastern Mediterranean Dendarus (Coleoptera: Tenebrionidae) sheds new light on the phylogeography of the Aegean area}, author = {Apostolos Trichas and Maria Smirli and Anna Papadopoulou and Ioannis Anastasiou and Bekir Keskin and Nikos Poulakakis}, url = {https://academic.oup.com/zoolinnean/advance-article/doi/10.1093/zoolinnean/zlaa022/5819313}, doi = {10.1093/zoolinnean/zlaa022}, issn = {0024-4082, 1096-3642}, year = {2020}, date = {2020-04-01}, urldate = {2020-09-14}, journal = {Zoological Journal of the Linnean Society}, pages = {zlaa022}, abstract = {Abstract The Aegean archipelago, as an ‘evolutionary laboratory of nature’, is an ideal model for research in phylogeography. In this area, the darkling beetles of the genus Dendarus (distributed from Morocco to the Caucasus) exhibit a high level of diversity with 36 species, 27 of which are island endemics. However, their taxonomy is complex and unstable, having undergone continuous revision to address extensive morphological and ecological plasticity. Here, we examine the phylogenetic relationships of 23 species from Greece and Turkey, using mitochondrial and nuclear DNA sequences and 61 morphological characters, to unveil their phylogeny in the Aegean. This helps to clarify phylogeographic scenarios and historical processes that shaped the observed patterns. The analyses reveal 13 distinct lineages with several para- and polyphyletic cases that correspond to three major phylogroups [south/south-east Aegean (D. foraminosus complex, D. rhodius, D. sporadicus, D. wettsteini); central to north Aegean, Turkey and mainland Greece (D. crenulatus, D. moesiacus group, D. sinuatus complex, D. stygius) and mainland Greece (D. messenius, D. paganettii)], indicating the need for further taxonomic re-evaluation. Lineage topology and phylogeography suggest a spatial and temporal sequence of geographic isolation, following either a vicariant or a dispersal model coincident with major palaeogeographic separations in the Aegean.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract The Aegean archipelago, as an ‘evolutionary laboratory of nature’, is an ideal model for research in phylogeography. In this area, the darkling beetles of the genus Dendarus (distributed from Morocco to the Caucasus) exhibit a high level of diversity with 36 species, 27 of which are island endemics. However, their taxonomy is complex and unstable, having undergone continuous revision to address extensive morphological and ecological plasticity. Here, we examine the phylogenetic relationships of 23 species from Greece and Turkey, using mitochondrial and nuclear DNA sequences and 61 morphological characters, to unveil their phylogeny in the Aegean. This helps to clarify phylogeographic scenarios and historical processes that shaped the observed patterns. The analyses reveal 13 distinct lineages with several para- and polyphyletic cases that correspond to three major phylogroups [south/south-east Aegean (D. foraminosus complex, D. rhodius, D. sporadicus, D. wettsteini); central to north Aegean, Turkey and mainland Greece (D. crenulatus, D. moesiacus group, D. sinuatus complex, D. stygius) and mainland Greece (D. messenius, D. paganettii)], indicating the need for further taxonomic re-evaluation. Lineage topology and phylogeography suggest a spatial and temporal sequence of geographic isolation, following either a vicariant or a dispersal model coincident with major palaeogeographic separations in the Aegean. |
Bariche, Michel; Al-Mabruk, Sara A A; Ateş, Maria Ayca; Büyük, Adnan; Crocetta, Fabio; Dritsas, Michail; Edde, Diala; Fortič, Ana; Gavriil, Elissavet; Gerovasileiou, Vasilis; Gökoğlu, Mehmet; Huseyinoglu, Fatih M; Karachle, Paraskevi K; Kleitou, Periklis; Kurt, Tuba Terbiyik; Langeneck, Joachim; Lardicci, Claudio; Lipej, Lovrenc; Pavloudi, Christina; Pinna, Maurizio; Rizgalla, Jamila; Özen, Mehmet Rüştü; Sedano, Francisco; Taşkin, Ergun; Yildiz, Gamze; Zangaro, Francesco New Alien Mediterranean Biodiversity Records 2020 Journal Article Mediterranean Marine Science, 21 (1), pp. 129, 2020, ISSN: 1791-6763, 1108-393X. @article{bariche_new_2020, title = {New Alien Mediterranean Biodiversity Records 2020}, author = {Michel Bariche and Sara A A Al-Mabruk and Maria Ayca Ateş and Adnan Büyük and Fabio Crocetta and Michail Dritsas and Diala Edde and Ana Fortič and Elissavet Gavriil and Vasilis Gerovasileiou and Mehmet Gökoğlu and Fatih M Huseyinoglu and Paraskevi K Karachle and Periklis Kleitou and Tuba Terbiyik Kurt and Joachim Langeneck and Claudio Lardicci and Lovrenc Lipej and Christina Pavloudi and Maurizio Pinna and Jamila Rizgalla and Mehmet Rüştü Özen and Francisco Sedano and Ergun Taşkin and Gamze Yildiz and Francesco Zangaro}, url = {https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/21987}, doi = {10.12681/mms.21987}, issn = {1791-6763, 1108-393X}, year = {2020}, date = {2020-04-01}, urldate = {2020-08-06}, journal = {Mediterranean Marine Science}, volume = {21}, number = {1}, pages = {129}, abstract = {The current article presents 18 new records from seven Mediterranean countries. These records include one rhodophyte, fournudibranchs, two crustaceans, one stingray and 10 bony fishes. They are grouped by country as follows: Lebanon - first record ofthe Striped bass Morone saxatilis, the stingray Himantura leoparda, the Areolate grouper Epinephelus areolatus and the Spot-finporcupinefish Diodon hystrix from various parts of the country; Turkey - first record of the invasive red alga Grateloupia turuturufrom the sea of Marmara (region of Bandırma), the sea slug Goniobranchus obsoletus and the crab Arcania brevifrons from theGulf of Antalya and the cladoceran Pleopis schmackeri from several locations along the Aegean Sea; Cyprus - first record of thealien sea slug Berthellina citrina from the region of Cape Greco and an observation of a butterflyfish Heniochus sp. from the northeasternside of the island; Greece - first record of the alien sea slug Anteaeolidiella lurana from the region of Heraklion in Creteand the record of the Atlantic spadefish Chaetodipterus faber and the Black surgeonfish Acanthurus cfr gahhm from SalaminaIsland; Slovenia - first record of the alien sea slug Thecacera pennigera from Izola; Italy - first record of the hybrid Striped bass(Morone saxatilis × Morone chrysops) from the northern Tyrrhenian Sea and a first record of the goldfish Carassius auratus fromthe region of Apulia; Libya - first record of the Red Sea goatfish Parupeneus forsskali and the African surgeonfish Acanthurusmonroviae, respectively from the eastern (Al-Tamimi area) and the western shore (Al-Khums area).}, keywords = {}, pubstate = {published}, tppubtype = {article} } The current article presents 18 new records from seven Mediterranean countries. These records include one rhodophyte, fournudibranchs, two crustaceans, one stingray and 10 bony fishes. They are grouped by country as follows: Lebanon - first record ofthe Striped bass Morone saxatilis, the stingray Himantura leoparda, the Areolate grouper Epinephelus areolatus and the Spot-finporcupinefish Diodon hystrix from various parts of the country; Turkey - first record of the invasive red alga Grateloupia turuturufrom the sea of Marmara (region of Bandırma), the sea slug Goniobranchus obsoletus and the crab Arcania brevifrons from theGulf of Antalya and the cladoceran Pleopis schmackeri from several locations along the Aegean Sea; Cyprus - first record of thealien sea slug Berthellina citrina from the region of Cape Greco and an observation of a butterflyfish Heniochus sp. from the northeasternside of the island; Greece - first record of the alien sea slug Anteaeolidiella lurana from the region of Heraklion in Creteand the record of the Atlantic spadefish Chaetodipterus faber and the Black surgeonfish Acanthurus cfr gahhm from SalaminaIsland; Slovenia - first record of the alien sea slug Thecacera pennigera from Izola; Italy - first record of the hybrid Striped bass(Morone saxatilis × Morone chrysops) from the northern Tyrrhenian Sea and a first record of the goldfish Carassius auratus fromthe region of Apulia; Libya - first record of the Red Sea goatfish Parupeneus forsskali and the African surgeonfish Acanthurusmonroviae, respectively from the eastern (Al-Tamimi area) and the western shore (Al-Khums area). |
Publications
2021 |
Sci Rep, 11 (1), pp. 373, 2021, ISSN: 2045-2322. |
Hydrobiologia, 848 (5), pp. 1163–1176, 2021, ISSN: 0018-8158, 1573-5117. |
Estuarine, Coastal and Shelf Science, 250 , pp. 107148, 2021, ISSN: 02727714. |
Reproduction of greater amberjack (Seriola dumerili) and other members of the family Carangidae Journal Article Rev Aquacult, pp. raq.12544, 2021, ISSN: 1753-5123, 1753-5131. |
Animals, 11 (2), pp. 392, 2021. |
Absorption and depletion of dietary administered praziquantel in greater amberjack, Seriola dumerili Journal Article Aquaculture, 535 , pp. 736354, 2021, ISSN: 00448486. |
Pathogens, 10 (2), pp. 100, 2021, ISSN: 2076-0817. |
Family-effects in the epigenomic response of red blood cells to a challenge test in the European sea bass (Dicentrarchus labrax, L.) Journal Article BMC Genomics, 22 (1), pp. 111, 2021, ISSN: 1471-2164. |
Genomic adaptation of Pseudomonas strains to acidity and antibiotics in hydrothermal vents at Kolumbo submarine volcano, Greece Journal Article Sci Rep, 11 (1), pp. 1336, 2021, ISSN: 2045-2322. |
Microorganisms, 9 (1), pp. 128, 2021, ISSN: 2076-2607. |
Lithistid Demosponges of Deep-Water Origin in Marine Caves of the North-Eastern Mediterranean Sea Journal Article Frontiers in Marine Science, 8 , 2021, ISSN: 2296-7745. |
Negligible risk of zoonotic anisakid nematodes in farmed fish from European mariculture, 2016 to 2018 Journal Article Eurosurveillance, 26 (2), 2021, ISSN: 1560-7917. |
Plastics in our ocean as transdisciplinary challenge Journal Article Marine Pollution Bulletin, 164 , pp. 112051, 2021, ISSN: 0025326X. |
Serum Ceramides as Prognostic Biomarkers of Large Thrombus Burden in Patients with STEMI: A Micro-Computed Tomography Study Journal Article Journal of Personalized Medicine, 11 (2), pp. 89, 2021. |
Developmental & Comparative Immunology, 114 , pp. 103803, 2021, ISSN: 0145305X. |
Trophic Diversity of a Fish Community Associated with a Caulerpa prolifera (Forsskål) Meadow in a Shallow Semi-Enclosed Embayment Journal Article JMSE, 9 (2), pp. 165, 2021, ISSN: 2077-1312. |
2020 |
Scientific Reports, 10 (1), pp. 12294, 2020, ISSN: 2045-2322. |
Mediterranean Marine Science, 21 (3), pp. 775, 2020, ISSN: 1791-6763, 1108-393X. |
BMC Cardiovascular Disorders, 20 (1), pp. 125, 2020, ISSN: 1471-2261. |
Aquaculture, 528 , pp. 735511, 2020, ISSN: 00448486. |
A Marine Biodiversity Observation Network for Genetic Monitoring of Hard-Bottom Communities (ARMS-MBON) Journal Article Frontiers in Marine Science, 7 , pp. 572680, 2020, ISSN: 2296-7745. |
Application of Non-Destructive Techniques on a Varve Sediment Record from Vouliagmeni Coastal Lake, Eastern Gulf of Corinth, Greece Journal Article Applied Sciences, 10 (22), pp. 8273, 2020, ISSN: 2076-3417. |
Macroinvertebrates as indicators of human disturbances on sandy beaches: A global review Journal Article Ecological Indicators, 118 , pp. 106764, 2020, ISSN: 1470160X. |
Really Onychocellids? Revisions and New Findings Increase the Astonishing Bryozoan Diversity of the Mediterranean Sea Journal Article Journal of Marine Science and Engineering, 8 (11), pp. 904, 2020, ISSN: 2077-1312. |
Vocal repertoire and consistency of call features in the meagre Argyrosomous regius (Asso, 1801) Journal Article PLOS ONE, 15 (11), pp. e0241792, 2020, ISSN: 1932-6203. |
Complete Genome Sequence of a Jumbo Bacteriophage, vB_pir03, against textitVibrio harveyi Journal Article Microbiology Resource Announcements, 9 (44), pp. e00910–20, /mra/9/44/MRA.00910–20.atom, 2020, ISSN: 2576-098X. |
Endocrine regulation of long-term enhancement of spermiation in meagre (Argyrosomus regius) with GnRHa controlled-delivery systems Journal Article General and Comparative Endocrinology, 297 , pp. 113549, 2020, ISSN: 00166480. |
Non-coding RNA Expression Patterns of Two Different Teleost Gonad Maturation Stages Journal Article Marine Biotechnology, 22 (5), pp. 683–695, 2020, ISSN: 1436-2228, 1436-2236. |
Detection of biogenic oil film near aquaculture sites seen by Sentinel-2 multispectral images Inproceedings Bostater, Charles R; Neyt, Xavier; Viallefont-Robinet, Françoise (Ed.): Remote Sensing of the Ocean, Sea Ice, Coastal Waters, and Large Water Regions 2020, pp. 4, SPIE, Online Only, United Kingdom, 2020, ISBN: 978-1-5106-3871-6 978-1-5106-3872-3. |
Folia Parasitologica, 67 , 2020, ISSN: 00155683, 18036465. |
High genetic diversity and variability of microbial communities in near-surface atmosphere of Crete island, Greece Journal Article Aerobiologia, 36 (3), pp. 341–353, 2020, ISSN: 0393-5965, 1573-3025. |
QTL for Stress and Disease Resistance in European Sea Bass, Dicentrarhus labrax L. Journal Article Animals, 10 (9), pp. 1668, 2020, ISSN: 2076-2615. |
Aquaculture Research, 51 (9), pp. 3551–3562, 2020, ISSN: 1355-557X, 1365-2109. |
Aquaculture Nutrition, pp. anu.13128, 2020, ISSN: 1353-5773, 1365-2095. |
Automated fish cage net inspection using image processing techniques Journal Article IET Image Processing, 14 (10), pp. 2028–2034, 2020, ISSN: 1751-9659, 1751-9667. |
Mediterranean Sea Literacy: When Ocean Literacy becomes region- specific Journal Article Mediterranean Marine Science, 2020, ISSN: 1791-6763, 1108-393X. |
Viral Metagenomic Content Reflects Seawater Ecological Quality in the Coastal Zone Journal Article Viruses, 12 (8), pp. 806, 2020. |
Journal of Fish Biology, 97 (1), pp. 314–317, 2020, ISSN: 0022-1112, 1095-8649. |
Infectious diseases and treatment solutions of farmed greater amberjack Seriola dumerili with particular emphasis in Mediterranean region Journal Article Reviews in Aquaculture, pp. raq.12476, 2020, ISSN: 1753-5123, 1753-5131. |
Pinna nobilis in the Greek seas (NE Mediterranean): on the brink of extinction? Journal Article Mediterranean Marine Science, 2020, ISSN: 1791-6763, 1108-393X. |
Composition and distribution patterns of eukaryotic microbial plankton in the ultra-oligotrophic Eastern Mediterranean Sea Journal Article Aquatic Microbial Ecology, 84 , pp. 155–173, 2020, ISSN: 0948-3055, (Publisher: Inter-Research Science Center). |
Coralligenous assemblages along their geographical distribution: Testing of concepts and implications for management Journal Article Aquatic Conservation: Marine and Freshwater Ecosystems, 30 , pp. 1578–1594, 2020, ISSN: 1052-7613, 1099-0755. |
Effect of water temperature on cellular stress responses in meagre (Argyrosomus regius) Journal Article Fish Physiology and Biochemistry, 46 (3), pp. 1075–1091, 2020, ISSN: 0920-1742, 1573-5168. |
Aquaculture Research, 51 (6), pp. 2443–2455, 2020, ISSN: 1355-557X, 1365-2109. |
Two new bacilladnaviruses associated with the diatom Haslea ostrearia Journal Article European Journal of Phycology, pp. 1–10, 2020, ISSN: 0967-0262, 1469-4433. |
Constrained by aliens, shifting landscape, or poor water quality? Factors affecting the persistence of amphibians in an urban pond network Journal Article Aquatic Conservation: Marine and Freshwater Ecosystems, 30 (5), pp. 1037–1049, 2020, ISSN: 1052-7613, 1099-0755. |
Aquaculture, 521 , pp. 735052, 2020, ISSN: 00448486. |
Aliens in the Aegean – a sea under siege (ALAS) Journal Article Research Ideas and Outcomes, 6 , pp. e53057, 2020, ISSN: 2367-7163. |
Zoological Journal of the Linnean Society, pp. zlaa022, 2020, ISSN: 0024-4082, 1096-3642. |
New Alien Mediterranean Biodiversity Records 2020 Journal Article Mediterranean Marine Science, 21 (1), pp. 129, 2020, ISSN: 1791-6763, 1108-393X. |