2025 |
Kotsiri, Mado; Kogiannou, Dimitra; Nikoloudaki, Chrisanthi; Kleidas, Ioannis; Dogrammatzi, Aikaterini; Karachle, Paraskevi K; Grigorakis, Kriton Seasonal Variation in Nutritional Value and Technical Quality of Lionfish (Pterois miles) from the Ionian and Aegean Seas Journal Article Foods, 14 (13), pp. 2353, 2025, ISSN: 2304-8158. @article{kotsiri_seasonal_2025, title = {Seasonal Variation in Nutritional Value and Technical Quality of Lionfish (Pterois miles) from the Ionian and Aegean Seas}, author = {Mado Kotsiri and Dimitra Kogiannou and Chrisanthi Nikoloudaki and Ioannis Kleidas and Aikaterini Dogrammatzi and Paraskevi K Karachle and Kriton Grigorakis}, url = {https://www.mdpi.com/2304-8158/14/13/2353 /wp-content/uploads/2025/07/2025-Kotsiri-foods-37.pdf }, doi = {10.3390/foods14132353}, issn = {2304-8158}, year = {2025}, date = {2025-07-18}, urldate = {2025-07-18}, journal = {Foods}, volume = {14}, number = {13}, pages = {2353}, abstract = {Lionfish (Pterois miles), an invasive species in the Mediterranean, pose ecological and socioeconomic challenges. This study examines the seasonal variation in the nutritional composition and technical quality of lionfish from the Ionian and Aegean Seas, evaluating their potential as a food resource. Fillets were high in protein (19.4%) and low in fat (2.0%), with significant seasonal differences in the Ionian Sea, where winter samples had higher lipid content. The fillet yield (28.4%) was satisfactory given the fish’s morphology. Fatty acid analysis confirmed lionfish as a valuable source of polyunsaturated fatty acids (PUFAs), particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), with EPA + DHA levels exceeding the recommended daily intake (119.2%). Seasonal variations in fatty acid composition were observed, including higher EPA in autumn and lower lipid nutritional quality in summer. Arachidonic acid (ARA) was also present at nutritionally significant levels (79.4 mg/100 g). The n3/n6 ratio (2.2) and favourable atherogenic and thrombogenic indices highlight its nutritional benefits. This is the first study to assess seasonal variations in the nutritional value and technical yield of lionfish in the Mediterranean, offering novel insights into its commercial valorisation. These findings support its promotion as a sustainable protein source and as a means of managing its invasive population.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Lionfish (Pterois miles), an invasive species in the Mediterranean, pose ecological and socioeconomic challenges. This study examines the seasonal variation in the nutritional composition and technical quality of lionfish from the Ionian and Aegean Seas, evaluating their potential as a food resource. Fillets were high in protein (19.4%) and low in fat (2.0%), with significant seasonal differences in the Ionian Sea, where winter samples had higher lipid content. The fillet yield (28.4%) was satisfactory given the fish’s morphology. Fatty acid analysis confirmed lionfish as a valuable source of polyunsaturated fatty acids (PUFAs), particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), with EPA + DHA levels exceeding the recommended daily intake (119.2%). Seasonal variations in fatty acid composition were observed, including higher EPA in autumn and lower lipid nutritional quality in summer. Arachidonic acid (ARA) was also present at nutritionally significant levels (79.4 mg/100 g). The n3/n6 ratio (2.2) and favourable atherogenic and thrombogenic indices highlight its nutritional benefits. This is the first study to assess seasonal variations in the nutritional value and technical yield of lionfish in the Mediterranean, offering novel insights into its commercial valorisation. These findings support its promotion as a sustainable protein source and as a means of managing its invasive population. |
Manga, Michael; Tominaga, Masako; Preine, Jonas; Ronge, Thomas A; Beethe, Sarah; Hübscher, Christian; McIntosh, Iona; Nomikou, Paraskevi; Kutterolf, Steffen; Druitt, Tim; Bernard, Alexis; Berthod, Carole; Chen, Hehe; Clark, Acacia; DeBari, Susan; Fernandez‐Perez, Tatiana I; Gertisser, Ralf; Johnston, Raymond M; Jones, Christopher K; Joshi, Batuk K; Kletetschka, Günther; Koukousioura, Olga; McCanta, Molly; Morris, Antony; Pank, Katharina; Peccia, Ally; Polymenakou, Paraskevi N; Woodhouse, Adam; Yamamoto, Yuzuru Low Heat Flow in the Anhydros Basin, Aegean Sea, Recorded by Deep Subsurface Temperatures Journal Article Geophysical Research Letters, 52 (13), pp. e2025GL115919, 2025, ISSN: 0094-8276, 1944-8007. @article{manga_low_2025, title = {Low Heat Flow in the Anhydros Basin, Aegean Sea, Recorded by Deep Subsurface Temperatures}, author = {Michael Manga and Masako Tominaga and Jonas Preine and Thomas A Ronge and Sarah Beethe and Christian Hübscher and Iona McIntosh and Paraskevi Nomikou and Steffen Kutterolf and Tim Druitt and Alexis Bernard and Carole Berthod and Hehe Chen and Acacia Clark and Susan DeBari and Tatiana I Fernandez‐Perez and Ralf Gertisser and Raymond M Johnston and Christopher K Jones and Batuk K Joshi and Günther Kletetschka and Olga Koukousioura and Molly McCanta and Antony Morris and Katharina Pank and Ally Peccia and Paraskevi N Polymenakou and Adam Woodhouse and Yuzuru Yamamoto}, url = {https://agupubs.onlinelibrary.wiley.com/doi/10.1029/2025GL115919 /wp-content/uploads/2025/07/2025-Manga-GRL-36.pdf}, doi = {10.1029/2025GL115919}, issn = {0094-8276, 1944-8007}, year = {2025}, date = {2025-07-16}, urldate = {2025-07-16}, journal = {Geophysical Research Letters}, volume = {52}, number = {13}, pages = {e2025GL115919}, abstract = {Abstract Subseafloor in situ temperatures in a drilled hole in the Anhydros Basin, Aegean Sea, measured during International Ocean Discovery Program (IODP) Expedition 398, yielded a low heat flow (textless0.023 W/m 2 ) despite active magmatism and rifting in the region. The coldest and highest temperatures were 13.9°C at 52.5 m below seafloor (mbsf) and 15.5°C for the deepest measurement at 360.4 mbsf, respectively. Comparison of a heat transfer model with measurements suggests that sea bottom temperatures during the last glacial period were up to 10°C cooler than Holocene temperatures. The magnitude of Holocene warming co‐varies with the geothermal heat flow: if the former goes up the latter goes up. Low heat flow may arise from lateral removal of heat through deeper formations by gravity driven advection of fluids. Tectonic separation of the northwestern Anhydros Basin from the Christiana‐Santorini‐Kolumbo volcanic field may lead to minimal magmatic influences on heat flow. , Plain Language Summary Temperatures in Earth's crust reveal the processes that create and transport heat. In the rifting Anhydros Basin in the Aegean Sea, north of the active South Aegean Volcanic Arc, International Ocean Discovery Program Expedition 398 measured cold subsurface temperatures in a borehole to depths exceeding 300 m below the seafloor. These temperatures record low heat flow and cold seafloor temperatures from the last glacial period. The low heat flow at shallow depths may be due to deeper fluid circulation that removes heat. Low heat flow further implies that there are no magma bodies within the crust in the northwestern part of the Anhydros Basin. , Key Points Heat flow is low in the Anhydros Basin, Aegean Sea Ocean bottom temperatures were cold during the last glacial Subsurface temperature records surface temperature changes and heat flow}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Subseafloor in situ temperatures in a drilled hole in the Anhydros Basin, Aegean Sea, measured during International Ocean Discovery Program (IODP) Expedition 398, yielded a low heat flow (textless0.023 W/m 2 ) despite active magmatism and rifting in the region. The coldest and highest temperatures were 13.9°C at 52.5 m below seafloor (mbsf) and 15.5°C for the deepest measurement at 360.4 mbsf, respectively. Comparison of a heat transfer model with measurements suggests that sea bottom temperatures during the last glacial period were up to 10°C cooler than Holocene temperatures. The magnitude of Holocene warming co‐varies with the geothermal heat flow: if the former goes up the latter goes up. Low heat flow may arise from lateral removal of heat through deeper formations by gravity driven advection of fluids. Tectonic separation of the northwestern Anhydros Basin from the Christiana‐Santorini‐Kolumbo volcanic field may lead to minimal magmatic influences on heat flow. , Plain Language Summary Temperatures in Earth's crust reveal the processes that create and transport heat. In the rifting Anhydros Basin in the Aegean Sea, north of the active South Aegean Volcanic Arc, International Ocean Discovery Program Expedition 398 measured cold subsurface temperatures in a borehole to depths exceeding 300 m below the seafloor. These temperatures record low heat flow and cold seafloor temperatures from the last glacial period. The low heat flow at shallow depths may be due to deeper fluid circulation that removes heat. Low heat flow further implies that there are no magma bodies within the crust in the northwestern part of the Anhydros Basin. , Key Points Heat flow is low in the Anhydros Basin, Aegean Sea Ocean bottom temperatures were cold during the last glacial Subsurface temperature records surface temperature changes and heat flow |
Dritsas, Panagiotis; Patsialou, Stefania; Kampantais, Dimitrios; Roussos, Efstratios; Kotzamanis, Yannis; Tekerlekopoulou, Athanasia; Vayenas, Dimitris V; Aggelis, George Investigating the Potential of Newly Isolated Microalgae Strains from the Ionian Sea (Greece) Cultured in an Open Raceway Pond Journal Article Applied Sciences, 15 (12), pp. 6680, 2025, ISSN: 2076-3417. @article{dritsas_investigating_2025, title = {Investigating the Potential of Newly Isolated Microalgae Strains from the Ionian Sea (Greece) Cultured in an Open Raceway Pond}, author = {Panagiotis Dritsas and Stefania Patsialou and Dimitrios Kampantais and Efstratios Roussos and Yannis Kotzamanis and Athanasia Tekerlekopoulou and Dimitris V Vayenas and George Aggelis}, url = {https://www.mdpi.com/2076-3417/15/12/6680 /wp-content/uploads/2025/07/2025-Dritsas-AppSci-35.pdf}, doi = {10.3390/app15126680}, issn = {2076-3417}, year = {2025}, date = {2025-07-07}, urldate = {2025-07-10}, journal = {Applied Sciences}, volume = {15}, number = {12}, pages = {6680}, abstract = {Microalgae are a subject of interest not only for fundamental research but for various biotechnological applications as well. In this study, the ability of newly isolated strains, i.e., Picochlorum costavermella VAS2.5, Picochlorum oklahomense SAG4.4, Microchloropsis gaditana VON5.3, and Nephroselmis pyriformis PAT2.7, to grow when cultured in an open raceway pond under laboratory conditions and produce various metabolites of high-added value was evaluated. N. pyriformis PAT2.7 and P. costavermella VAS2.5 were the greatest in biomass production (exceeding 0.4 g/L), while P. costavermella VAS2.5 and M. gaditana VON5.3 were the greatest in lipid production (reaching approximately 18%, wt/wt). The lipid fraction of glycolipids and sphingolipids was predominant (43.6–55.4%, wt/wt), followed by neutral lipids (27.1–50.1%, wt/wt) and phospholipids (6.9–17.4%, wt/wt). Picochlora and M. gaditana VON5.3 lipids were rich in Δ5,8,11,14,17C20:5 and/or Δ9,12,15C18:3, while N. pyriformis PAT2.7 synthesized Δ9C16:1 in large quantities (30–40%, wt/wt). All strains showed remarkable yields in polysaccharide and protein production, demonstrated a well-balanced amino acid profile, and synthesized pigments in amounts comparable to other studies. The biochemical profiles of these strains showcased their suitability for use primarily in the aquaculture sector.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Microalgae are a subject of interest not only for fundamental research but for various biotechnological applications as well. In this study, the ability of newly isolated strains, i.e., Picochlorum costavermella VAS2.5, Picochlorum oklahomense SAG4.4, Microchloropsis gaditana VON5.3, and Nephroselmis pyriformis PAT2.7, to grow when cultured in an open raceway pond under laboratory conditions and produce various metabolites of high-added value was evaluated. N. pyriformis PAT2.7 and P. costavermella VAS2.5 were the greatest in biomass production (exceeding 0.4 g/L), while P. costavermella VAS2.5 and M. gaditana VON5.3 were the greatest in lipid production (reaching approximately 18%, wt/wt). The lipid fraction of glycolipids and sphingolipids was predominant (43.6–55.4%, wt/wt), followed by neutral lipids (27.1–50.1%, wt/wt) and phospholipids (6.9–17.4%, wt/wt). Picochlora and M. gaditana VON5.3 lipids were rich in Δ5,8,11,14,17C20:5 and/or Δ9,12,15C18:3, while N. pyriformis PAT2.7 synthesized Δ9C16:1 in large quantities (30–40%, wt/wt). All strains showed remarkable yields in polysaccharide and protein production, demonstrated a well-balanced amino acid profile, and synthesized pigments in amounts comparable to other studies. The biochemical profiles of these strains showcased their suitability for use primarily in the aquaculture sector. |
Vasilaki, Antigoni; Nengas, Ioannis; Fountoulaki, Eleni; Henry, Morgane; Kogiannou, Dimitra; Nikoloudaki, Chrysanthi; Chronopoulos, Petros; Karapanagiotidis, Ioannis T; Mente, Elena Aquaculture, 609 , pp. 742820, 2025, ISSN: 0044-8486. @article{vasilaki_nutritional_2025, title = {Nutritional enhancement of lupin meal (\textit{Lupinus albus}), through fermentation with textitSaccharomyces cerevisiae, as plant protein ingredient in aquafeeds for the European sea bass (\textit{Dicentrarchus labrax}).}, author = {Antigoni Vasilaki and Ioannis Nengas and Eleni Fountoulaki and Morgane Henry and Dimitra Kogiannou and Chrysanthi Nikoloudaki and Petros Chronopoulos and Ioannis T Karapanagiotidis and Elena Mente}, url = {https://www.sciencedirect.com/science/article/pii/S0044848625007069 /wp-content/uploads/2025/07/2025-Vasilaki-AQUA-34.pdf}, doi = {10.1016/j.aquaculture.2025.742820}, issn = {0044-8486}, year = {2025}, date = {2025-07-04}, urldate = {2025-07-09}, journal = {Aquaculture}, volume = {609}, pages = {742820}, abstract = {Legumes contain antinutritional factors that restrict their use in fish nutrition, however, biotechnological methods such as solid-state fermentation can improve their nutritional profile, positioning them as a sustainable alternative for aquafeed formulation. This research evaluated the feasibility of replacing soybean meal with Saccharomyces cerevisiae-fermented lupin meal (Lupinus albus) in feed formulations for European sea bass (Dicentrarchus labrax). Four diets were produced; one commercial type diet (FRL0) and three diets with partial (FRL10, FRL12.5) or complete (FRL15) replacement of soybean meal by fermented lupin meal. European sea bass juveniles, with an initial average weight: 18.9 g, were fed the experimental diets for 71 days in triplicate groups. Results indicated no adverse effects on key production indices when soybean meal was replaced partially or totally. Fermented lupin meal linearly improved feed utilization. Apparent digestibility coefficient of protein significantly increased in groups fed 12.5 % and 15 % fermented lupin, as did apparent digestibility coefficient of fat, compared to the FRL0 group. Trypsin activity in pyloric caeca was statistically elevated in the fermented lupin groups compare to FRL0 group. Whole-body composition, amino acid content, and deposition showed no significant differences among groups. Fermented lupin did not suppress the immune system, and a slight immunostimulatory effect was observed at a 12.5 % inclusion level. No histopathological alterations were detected. Overall, the results demonstrated the efficacy of solid-state fermentation process in enhancing lupin meal, highlighting its potential as a sustainable replacement for soybean meal in feed formulations for Mediterranean marine aquaculture species such us D. labrax.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Legumes contain antinutritional factors that restrict their use in fish nutrition, however, biotechnological methods such as solid-state fermentation can improve their nutritional profile, positioning them as a sustainable alternative for aquafeed formulation. This research evaluated the feasibility of replacing soybean meal with Saccharomyces cerevisiae-fermented lupin meal (Lupinus albus) in feed formulations for European sea bass (Dicentrarchus labrax). Four diets were produced; one commercial type diet (FRL0) and three diets with partial (FRL10, FRL12.5) or complete (FRL15) replacement of soybean meal by fermented lupin meal. European sea bass juveniles, with an initial average weight: 18.9 g, were fed the experimental diets for 71 days in triplicate groups. Results indicated no adverse effects on key production indices when soybean meal was replaced partially or totally. Fermented lupin meal linearly improved feed utilization. Apparent digestibility coefficient of protein significantly increased in groups fed 12.5 % and 15 % fermented lupin, as did apparent digestibility coefficient of fat, compared to the FRL0 group. Trypsin activity in pyloric caeca was statistically elevated in the fermented lupin groups compare to FRL0 group. Whole-body composition, amino acid content, and deposition showed no significant differences among groups. Fermented lupin did not suppress the immune system, and a slight immunostimulatory effect was observed at a 12.5 % inclusion level. No histopathological alterations were detected. Overall, the results demonstrated the efficacy of solid-state fermentation process in enhancing lupin meal, highlighting its potential as a sustainable replacement for soybean meal in feed formulations for Mediterranean marine aquaculture species such us D. labrax. |
Droubogiannis, Stavros; Triga, Andriana; Pavlidis, Michail; Katharios, Pantelis Genome sequences of textitVibrio harveyi VH2 and textitVibrio harveyi Vhp1-sp Journal Article Microbiology Resource Announcements, pp. e00087–25, 2025, ISSN: 2576-098X. @article{droubogiannis_genome_2025, title = {Genome sequences of textitVibrio harveyi VH2 and textitVibrio harveyi Vhp1-sp}, author = {Stavros Droubogiannis and Andriana Triga and Michail Pavlidis and Pantelis Katharios}, editor = {Frank J Stewart}, url = {https://journals.asm.org/doi/10.1128/mra.00087-25 /wp-content/uploads/2025/07/2025-Droubogiannis-MIcro-RA-33.pdf}, doi = {10.1128/mra.00087-25}, issn = {2576-098X}, year = {2025}, date = {2025-06-30}, urldate = {2025-07-01}, journal = {Microbiology Resource Announcements}, pages = {e00087--25}, abstract = {ABSTRACT Vibrio harveyi is a major bacterial pathogen of marine aquatic animals causing significant economic losses in aquaculture. Here, we present the complete genomes of two pathogenic strains, VH2 (5.84 Mb, 44.99% GC) and Vhp1-sp (6.30 Mb, 44.98% GC), which encode numerous virulence factors, resistance genes, and genomic islands.}, keywords = {}, pubstate = {published}, tppubtype = {article} } ABSTRACT Vibrio harveyi is a major bacterial pathogen of marine aquatic animals causing significant economic losses in aquaculture. Here, we present the complete genomes of two pathogenic strains, VH2 (5.84 Mb, 44.99% GC) and Vhp1-sp (6.30 Mb, 44.98% GC), which encode numerous virulence factors, resistance genes, and genomic islands. |
Lancerotto, Stefano; Fakriadis, Ioannis; Papadaki, Maria; Sigelaki, Irini; Giménez, Ignacio; Genoves, Jose Vicente Roig; Meloni, Andrea; Mylonas, Constantinos C Aquaculture, 609 , pp. 742795, 2025, ISSN: 00448486. @article{lancerotto_administration_2025, title = {Administration of single-chain recombinant follicle-stimulating hormone (sdrFsh) and luteinizing hormone (sdrLh) stimulates spermatogenesis, but not vitellogenesis in pre-pubertal greater amberjack (\textit{Seriola dumerili})}, author = {Stefano Lancerotto and Ioannis Fakriadis and Maria Papadaki and Irini Sigelaki and Ignacio Giménez and Jose Vicente Roig Genoves and Andrea Meloni and Constantinos C Mylonas}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848625006817 /wp-content/uploads/2025/06/2025-Lancerotto-AQUA-pre-print32.pdf}, doi = {10.1016/j.aquaculture.2025.742795}, issn = {00448486}, year = {2025}, date = {2025-06-24}, urldate = {2025-06-24}, journal = {Aquaculture}, volume = {609}, pages = {742795}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Keklikoglou, Kleoniki; Mandalakis, Manolis; Fanouraki, Eleftheria; Anastasiou, Thekla I; Kagiampaki, Eirini; Vasileiadou, Katerina; Pavloudi, Christina; Arvanitidis, Christos; Pavlidis, Michail Disrupted darkness: the impact of anthropogenic light at night on melatonin secretion of Hermodice carunculata (Polychaeta, Annelida) Journal Article Photochemical & Photobiological Sciences, 24 (6), pp. 1031–1040, 2025, ISSN: 1474-905X, 1474-9092. @article{keklikoglou_disrupted_2025, title = {Disrupted darkness: the impact of anthropogenic light at night on melatonin secretion of \textit{Hermodice carunculata (Polychaeta, Annelida)}}, author = {Kleoniki Keklikoglou and Manolis Mandalakis and Eleftheria Fanouraki and Thekla I Anastasiou and Eirini Kagiampaki and Katerina Vasileiadou and Christina Pavloudi and Christos Arvanitidis and Michail Pavlidis}, url = {https://link.springer.com/10.1007/s43630-025-00744-5 /wp-content/uploads/2025/06/2025-Keklikoglou-PP-Sci-31.pdf}, doi = {10.1007/s43630-025-00744-5}, issn = {1474-905X, 1474-9092}, year = {2025}, date = {2025-06-20}, urldate = {2025-06-20}, journal = {Photochemical & Photobiological Sciences}, volume = {24}, number = {6}, pages = {1031--1040}, abstract = {Abstract Anthropogenic light at night (ALAN) can have serious impacts on marine environments. Several studies have demonstrated that ALAN disrupts melatonin production, a hormone critical for regulating circadian rhythm. In this study, the effects of ALAN on melatonin and two of its related indolamines were investigated in the annelid Hermodice carunculata . Specifically, melatonin, serotonin and tryptamine levels were measured every three hours over a 24 h period in the heads of the annelids maintained under constant light and a 12 h light/12 h dark photoperiod, representing control conditions. Melatonin concentration was quantified using an enzyme immunoassay, while serotonin and tryptamine were analyzed by liquid chromatography–tandem mass spectrometry. Melatonin levels in annelid heads remained relatively constant with a pronounced peak at 11:00. A similar pattern was observed under constant light, but the melatonin peak shifted to 14:00. However, serotonin and tryptamine did not exhibit any significant diurnal variations due to constant light exposure. These findings suggest that melatonin secretion in H. carunculata is sensitive to ALAN, whereas its related indolamines are potentially not. The disruption of H. carunculata' s melatonin secretion pattern may affect its night-time behavior and reproduction, highlighting the need for further studies to assess the ecological effects of ALAN on various marine invertebrates. Graphical abstract}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Anthropogenic light at night (ALAN) can have serious impacts on marine environments. Several studies have demonstrated that ALAN disrupts melatonin production, a hormone critical for regulating circadian rhythm. In this study, the effects of ALAN on melatonin and two of its related indolamines were investigated in the annelid Hermodice carunculata . Specifically, melatonin, serotonin and tryptamine levels were measured every three hours over a 24 h period in the heads of the annelids maintained under constant light and a 12 h light/12 h dark photoperiod, representing control conditions. Melatonin concentration was quantified using an enzyme immunoassay, while serotonin and tryptamine were analyzed by liquid chromatography–tandem mass spectrometry. Melatonin levels in annelid heads remained relatively constant with a pronounced peak at 11:00. A similar pattern was observed under constant light, but the melatonin peak shifted to 14:00. However, serotonin and tryptamine did not exhibit any significant diurnal variations due to constant light exposure. These findings suggest that melatonin secretion in H. carunculata is sensitive to ALAN, whereas its related indolamines are potentially not. The disruption of H. carunculata' s melatonin secretion pattern may affect its night-time behavior and reproduction, highlighting the need for further studies to assess the ecological effects of ALAN on various marine invertebrates. Graphical abstract |
Oikonomou, Stavroula; Manousi, Domniki; Dimitroglou, Arkadios; Kazlari, Zoi; Loukovitis, Dimitrios; Papanna, Kantham; Tzokas, Konstantinos; Katribouzas, Nikos; Papaharisis, Leonidas; Tsigenopoulos, Costas S; Chatziplis, Dimitrios Validation of QTL associated with resistance to Lernanthropus kroyeri in European seabass Journal Article Aquaculture, 608 , pp. 742763, 2025, ISSN: 00448486. @article{oikonomou_validation_2025, title = {Validation of QTL associated with resistance to Lernanthropus kroyeri in European seabass}, author = {Stavroula Oikonomou and Domniki Manousi and Arkadios Dimitroglou and Zoi Kazlari and Dimitrios Loukovitis and Kantham Papanna and Konstantinos Tzokas and Nikos Katribouzas and Leonidas Papaharisis and Costas S Tsigenopoulos and Dimitrios Chatziplis}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848625006490 /wp-content/uploads/2025/06/2025-Oikonomou-AQUA-30.pdf}, doi = {10.1016/j.aquaculture.2025.742763}, issn = {00448486}, year = {2025}, date = {2025-06-18}, urldate = {2025-06-18}, journal = {Aquaculture}, volume = {608}, pages = {742763}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Rizzo, Lucia; Fernández, Tomás Vega; Necci, Francesca; Grelaud, Michaël; Ziveri, Patrizia; Gerovasileiou, Vasilis; Rossi, Sergio Mediterranean Marine Science, 26 (2), pp. 327–340, 2025, ISSN: 1791-6763, 1108-393X. @article{rizzo_can_2025, title = {Can Marine Animal Forests benefit from existing conservation measures? A systematic approach towards the identification of protected sessile benthic species in the Mediterranean Sea}, author = {Lucia Rizzo and Tomás Vega Fernández and Francesca Necci and Michaël Grelaud and Patrizia Ziveri and Vasilis Gerovasileiou and Sergio Rossi}, url = {https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/38550 /wp-content/uploads/2025/06/2025-Rizzo-MMS-29.pdf}, doi = {10.12681/mms.38550}, issn = {1791-6763, 1108-393X}, year = {2025}, date = {2025-06-17}, urldate = {2025-06-17}, journal = {Mediterranean Marine Science}, volume = {26}, number = {2}, pages = {327--340}, abstract = {Some marine benthic invertebrates increase the structural complexity of the seabed, thereby providing suitable habitats to several associated species, resulting in biodiversity hotspots. Marine Animal Forests (MAFs) encompass a set of the most important marine benthic habitats into which diversified sessile suspension feeders like sponges, corals, sea pens, tube worms, bivalves, bryozoans and ascidians occur. Such a mix of sessile species brings characteristic assemblages and supports important ecosystem functions. In the last decades, some species which form MAFs have been the object of international conventions, EU directives, and national policies aiming to address natural and human-induced disturbances. Effective conservation, monitoring, and restoration actions require summarizing the available information to include MAFs in conservation plans. In the present work, the main international policies for the protection of coastal and marine fauna were screened in order to provide a list of protected species which form Mediterranean MAFs. These international normative documents include the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES), the Convention on the Conservation of European Wildlife and Habitats (Bern Convention), the European Habitats Directive, and the Protocol concerning Specially Protected Areas and Biological Diversity in the Mediterranean (SPA/BD Protocol) of the Barcelona Convention. The analysis of the normative items revealed that 62 Mediterranean potential MAF forming species have been included in one or more legal annexes as protected species. These species belong to different phyla, including Porifera, Cnidaria, Bryozoa and Mollusca. A wider view was proposed by the International Union for Conservation of Nature (IUCN), setting the bases of a legal acknowledgement of the MAFs as one of the largest biomes on Earth. This study represents a first step to obtain a baseline of MAF legal protection framework in order to support the further implementation of management measures aimed at increasing the effective protection of MAFs. Reporting the status of the Mediterranean MAF species that should be considered in management plans and conservation measures will be crucial for policymakers, as well as for mitigating current and future impacts on these distinctive marine environments.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Some marine benthic invertebrates increase the structural complexity of the seabed, thereby providing suitable habitats to several associated species, resulting in biodiversity hotspots. Marine Animal Forests (MAFs) encompass a set of the most important marine benthic habitats into which diversified sessile suspension feeders like sponges, corals, sea pens, tube worms, bivalves, bryozoans and ascidians occur. Such a mix of sessile species brings characteristic assemblages and supports important ecosystem functions. In the last decades, some species which form MAFs have been the object of international conventions, EU directives, and national policies aiming to address natural and human-induced disturbances. Effective conservation, monitoring, and restoration actions require summarizing the available information to include MAFs in conservation plans. In the present work, the main international policies for the protection of coastal and marine fauna were screened in order to provide a list of protected species which form Mediterranean MAFs. These international normative documents include the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES), the Convention on the Conservation of European Wildlife and Habitats (Bern Convention), the European Habitats Directive, and the Protocol concerning Specially Protected Areas and Biological Diversity in the Mediterranean (SPA/BD Protocol) of the Barcelona Convention. The analysis of the normative items revealed that 62 Mediterranean potential MAF forming species have been included in one or more legal annexes as protected species. These species belong to different phyla, including Porifera, Cnidaria, Bryozoa and Mollusca. A wider view was proposed by the International Union for Conservation of Nature (IUCN), setting the bases of a legal acknowledgement of the MAFs as one of the largest biomes on Earth. This study represents a first step to obtain a baseline of MAF legal protection framework in order to support the further implementation of management measures aimed at increasing the effective protection of MAFs. Reporting the status of the Mediterranean MAF species that should be considered in management plans and conservation measures will be crucial for policymakers, as well as for mitigating current and future impacts on these distinctive marine environments. |
Kouvara, Konstantina; Lazou-Laskaridis, Evi; Xirotagarou, Peggy; Christodoulou, Dimitris; Dimas, Xenophon; Geraga, Maria; Giovos, Ioannis; Charitou, Anastasia; Gerovasileiou, Vasilis; Galgani, Francois; Papatheodorou, George Marine Pollution Bulletin, 217 , pp. 118109, 2025, ISSN: 0025326X. @article{kouvara_assessing_2025, title = {Assessing marine litter and its ecological impact on the seafloor of Thermaikos Gulf (NE Mediterranean Sea, Greece): Insights from ROV and diver surveys}, author = {Konstantina Kouvara and Evi Lazou-Laskaridis and Peggy Xirotagarou and Dimitris Christodoulou and Xenophon Dimas and Maria Geraga and Ioannis Giovos and Anastasia Charitou and Vasilis Gerovasileiou and Francois Galgani and George Papatheodorou}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0025326X25005843 /wp-content/uploads/2025/06/2025-Kouvara-MPB-nonOPEN-28.pdf}, doi = {10.1016/j.marpolbul.2025.118109}, issn = {0025326X}, year = {2025}, date = {2025-06-16}, urldate = {2025-06-16}, journal = {Marine Pollution Bulletin}, volume = {217}, pages = {118109}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Asimaki, Adamantia; Psofakis, Pier; Gkalogianni, Elli-Zafeiria; Katouni, Aikaterini-Maria; Berillis, Panagiotis; Kormas, Konstantinos A; Rumbos, Christos I; Athanassiou, Christos G; Vasilaki, Antigoni; Fountoulaki, Eleni; Henry, Morgane; Mente, Eleni; Gisbert, Enric; Karapanagiotidis, Ioannis T Aquaculture Nutrition, 2025 (1), pp. 8885509, 2025, ISSN: 1353-5773, 1365-2095. @article{asimaki_effects_2025, title = {The Effects of Dietary Fishmeal Substitution by Full‐Fat and Defatted textitZophobas morio Larvae Meals on Juvenile Gilthead Seabream (\textit{Sparus aurata}): An Integrative Approach}, author = {Adamantia Asimaki and Pier Psofakis and Elli-Zafeiria Gkalogianni and Aikaterini-Maria Katouni and Panagiotis Berillis and Konstantinos A Kormas and Christos I Rumbos and Christos G Athanassiou and Antigoni Vasilaki and Eleni Fountoulaki and Morgane Henry and Eleni Mente and Enric Gisbert and Ioannis T Karapanagiotidis}, editor = {Lee Seong Wei}, url = {https://onlinelibrary.wiley.com/doi/10.1155/anu/8885509 /wp-content/uploads/2025/06/2025-Asimaki-Aq-Nutr-27.pdf}, doi = {10.1155/anu/8885509}, issn = {1353-5773, 1365-2095}, year = {2025}, date = {2025-06-10}, urldate = {2025-06-10}, journal = {Aquaculture Nutrition}, volume = {2025}, number = {1}, pages = {8885509}, abstract = {This study evaluated the dietary fishmeal substitution by full‐fat (FF) and defatted (DF) Zophobas morio meals regarding growth, feed efficiency, proximate and fatty acid compositions, digestive enzymes activities, histology and midgut microbiota in gilthead seabream ( Sparus aurata ). Juveniles initially weighing 3.4 g were distributed to triplicate groups and fed at satiation six isonitrogenous (8.41%) and isocaloric (21 Mj/kg) diets for 100 days. An insect meal‐free diet was the control (CTRL), two diets contained a FF Z. morio meal at 49 g/kg (FF‐49) and 97 g/kg (FF‐97), and three diets contained a DF Z. morio meal at 58 g/kg (DF‐58), 116 g/kg (DF‐116) and 174 g/kg (DF‐174) at the expense of fishmeal. Neither the form nor the inclusion level of Z. morio meals affected the feed intake of fish denoting a similar acceptability to that of fishmeal. Fish survival, growth and feed efficiency were not impaired by all dietary inclusion levels of Z. morio meals. Proximate composition of fish was altered but without indicating a clear correlation with the form or inclusion level of Z. morio . Increasing inclusions of both forms of Z. morio meals tended to decrease 22:6n‐3, 20:5n‐3, 18:3n‐3 and 18:2n‐6 levels in fish tissues. All fish exhibited similar proteolytic enzyme activities, but the increasing inclusions of both insect meal forms led to gradual increases in the lipase and α‐amylase activities indicating a compensatory mechanism for lipid and carbohydrate digestion. The use of Z. morio meals led to some mild histomorphological changes in the intestine and liver that were more pronounced in fish fed the FF form at the highest inclusion level. Midgut bacterial communities of the groups were similar and dominated by potentially beneficial members of Saccharimonadales and Rhodobacteraceae , except FF‐97 fish that had high abundances of Legionella ‐ and Pandoraea ‐like bacteria. To conclude, Z. morio meal, either FF or DF, is a suitable insect protein for fishmeal substitution towards more sustainable aquafeeds for S. aurata .}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study evaluated the dietary fishmeal substitution by full‐fat (FF) and defatted (DF) Zophobas morio meals regarding growth, feed efficiency, proximate and fatty acid compositions, digestive enzymes activities, histology and midgut microbiota in gilthead seabream ( Sparus aurata ). Juveniles initially weighing 3.4 g were distributed to triplicate groups and fed at satiation six isonitrogenous (8.41%) and isocaloric (21 Mj/kg) diets for 100 days. An insect meal‐free diet was the control (CTRL), two diets contained a FF Z. morio meal at 49 g/kg (FF‐49) and 97 g/kg (FF‐97), and three diets contained a DF Z. morio meal at 58 g/kg (DF‐58), 116 g/kg (DF‐116) and 174 g/kg (DF‐174) at the expense of fishmeal. Neither the form nor the inclusion level of Z. morio meals affected the feed intake of fish denoting a similar acceptability to that of fishmeal. Fish survival, growth and feed efficiency were not impaired by all dietary inclusion levels of Z. morio meals. Proximate composition of fish was altered but without indicating a clear correlation with the form or inclusion level of Z. morio . Increasing inclusions of both forms of Z. morio meals tended to decrease 22:6n‐3, 20:5n‐3, 18:3n‐3 and 18:2n‐6 levels in fish tissues. All fish exhibited similar proteolytic enzyme activities, but the increasing inclusions of both insect meal forms led to gradual increases in the lipase and α‐amylase activities indicating a compensatory mechanism for lipid and carbohydrate digestion. The use of Z. morio meals led to some mild histomorphological changes in the intestine and liver that were more pronounced in fish fed the FF form at the highest inclusion level. Midgut bacterial communities of the groups were similar and dominated by potentially beneficial members of Saccharimonadales and Rhodobacteraceae , except FF‐97 fish that had high abundances of Legionella ‐ and Pandoraea ‐like bacteria. To conclude, Z. morio meal, either FF or DF, is a suitable insect protein for fishmeal substitution towards more sustainable aquafeeds for S. aurata . |
Okeleye, Oluwabusayo Israel; Majstorovic, Jovana; Sudharaka, Anupa; Triga, Adriana; Mandalakis, Manolis; Katharios, Pantelis; Mladineo, Ivona Common carp (Cyprinus carpio) blood cells are modulated by a parasitic antimicrobial peptide, A-2S and Edwardsiella spp. strains Journal Article Fish & Shellfish Immunology, 164 , pp. 110427, 2025, ISSN: 10504648. @article{okeleye_common_2025, title = {Common carp (\textit{Cyprinus carpio}) blood cells are modulated by a parasitic antimicrobial peptide, A-2S and Edwardsiella spp. strains}, author = {Oluwabusayo Israel Okeleye and Jovana Majstorovic and Anupa Sudharaka and Adriana Triga and Manolis Mandalakis and Pantelis Katharios and Ivona Mladineo}, url = {https://linkinghub.elsevier.com/retrieve/pii/S105046482500316X /wp-content/uploads/2025/06/2025-Busayo-FSI-26.pdf}, doi = {10.1016/j.fsi.2025.110427}, issn = {10504648}, year = {2025}, date = {2025-06-06}, urldate = {2025-06-06}, journal = {Fish & Shellfish Immunology}, volume = {164}, pages = {110427}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Cascarano, Maria Chiara; Sevasti, Papadogiorgaki; Katharios, Pantelis Ultrastructural Insights Into a textitCandidatus Parilichlamydia sp. Infection of Gill Goblet Cells in Greater Amberjack Journal Article Journal of Fish Diseases, pp. e14146, 2025, ISSN: 0140-7775, 1365-2761. @article{cascarano_ultrastructural_2025, title = {Ultrastructural Insights Into a textitCandidatus Parilichlamydia sp. Infection of Gill Goblet Cells in Greater Amberjack}, author = {Maria Chiara Cascarano and Papadogiorgaki Sevasti and Pantelis Katharios}, url = {https://onlinelibrary.wiley.com/doi/10.1111/jfd.14146 /wp-content/uploads/2025/05/2025-Cascarano-JFD-25.pdf}, doi = {10.1111/jfd.14146}, issn = {0140-7775, 1365-2761}, year = {2025}, date = {2025-05-28}, urldate = {2025-05-28}, journal = {Journal of Fish Diseases}, pages = {e14146}, abstract = {ABSTRACT Despite recent genomic studies and increased molecular data, epitheliocystis remains an enigmatic fish disease with no experimental in vitro or in vivo models to aid the advancement of research. In this study, we revert to a classical microscopical approach and screen with the electron microscope the epitheliocystis lesions caused by a Ca . Parilichlamydia sp., infecting mucus cells in Greater amberjack. We report distinct morphological features of this bacterial family, characterised by Intermediate Bodies that closely resemble those of previously described Candidatus similchlamydia , and Elementary Bodies that exhibit morphological similarities to Chlamydia trachomatis . We describe the characteristics of a novel Chlamydial Inclusion Membrane (IM) type, with abundant interdigitations, possibly shaped by fusion of the IM with cytoplasmic vesicles, and moreover discuss the presence of multivesicular bodies in the infected cell. Our observation of immune cells in the infected areas indicates an interaction of macrophages with infected cells, a role for granular cells as pathogens reservoirs and an active phagoptosis process in the nearby areas, overall shedding light on cellular immune processes characterising these infections in fish hosts.}, keywords = {}, pubstate = {published}, tppubtype = {article} } ABSTRACT Despite recent genomic studies and increased molecular data, epitheliocystis remains an enigmatic fish disease with no experimental in vitro or in vivo models to aid the advancement of research. In this study, we revert to a classical microscopical approach and screen with the electron microscope the epitheliocystis lesions caused by a Ca . Parilichlamydia sp., infecting mucus cells in Greater amberjack. We report distinct morphological features of this bacterial family, characterised by Intermediate Bodies that closely resemble those of previously described Candidatus similchlamydia , and Elementary Bodies that exhibit morphological similarities to Chlamydia trachomatis . We describe the characteristics of a novel Chlamydial Inclusion Membrane (IM) type, with abundant interdigitations, possibly shaped by fusion of the IM with cytoplasmic vesicles, and moreover discuss the presence of multivesicular bodies in the infected cell. Our observation of immune cells in the infected areas indicates an interaction of macrophages with infected cells, a role for granular cells as pathogens reservoirs and an active phagoptosis process in the nearby areas, overall shedding light on cellular immune processes characterising these infections in fish hosts. |
Siaperas, Romanos; Taxeidis, George; Gioti, Anastasia; Nikolaivits, Efstratios; Topakas, Evangelos Multi-omics insights into the response of Aspergillus parasiticus to long-chain alkanes in relation to polyethylene modification Journal Article Environmental Pollution, 376 , pp. 126386, 2025, ISSN: 02697491. @article{siaperas_multi-omics_2025, title = {Multi-omics insights into the response of Aspergillus parasiticus to long-chain alkanes in relation to polyethylene modification}, author = {Romanos Siaperas and George Taxeidis and Anastasia Gioti and Efstratios Nikolaivits and Evangelos Topakas}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0269749125007596 /wp-content/uploads/2025/05/2025-Siaperas-EnvPol-24.pdf}, doi = {10.1016/j.envpol.2025.126386}, issn = {02697491}, year = {2025}, date = {2025-05-22}, urldate = {2025-05-22}, journal = {Environmental Pollution}, volume = {376}, pages = {126386}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Díaz-Avello, Ulises G; Skouridou, Vasso; Shkembi, Xhensila; Reverté, Jaume; Mandalakis, Manolis; Peristeraki, Panagiota; Campàs, Mònica; O'Sullivan, Ciara K Aptamer-antibody sandwich lateral flow test for rapid visual detection of tetrodotoxin in pufferfish Journal Article Science of The Total Environment, 978 , pp. 179419, 2025, ISSN: 00489697. @article{diaz-avello_aptamer-antibody_2025, title = {Aptamer-antibody sandwich lateral flow test for rapid visual detection of tetrodotoxin in pufferfish}, author = {Ulises G Díaz-Avello and Vasso Skouridou and Xhensila Shkembi and Jaume Reverté and Manolis Mandalakis and Panagiota Peristeraki and Mònica Campàs and Ciara K O'Sullivan}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0048969725010563 /wp-content/uploads/2025/05/2025-Díaz-Avello-TTX_LFA-test-DRAFT-SUBMITTED-23.pdf}, doi = {10.1016/j.scitotenv.2025.179419}, issn = {00489697}, year = {2025}, date = {2025-05-20}, urldate = {2025-05-20}, journal = {Science of The Total Environment}, volume = {978}, pages = {179419}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ventriglia, Gianluca; Duncan, Neil; Giménez, Ignacio; Mylonas, Constantinos C; Pousis, Chrysovalentinos; Corriero, Aldo; Zupa, Rosa Spermatogenesis advancement in pre-pubertal meagre Argyrosomus regius treated with recombinant gonadotropins Journal Article Scientific Reports, 15 (1), pp. 15113, 2025, ISSN: 2045-2322. @article{ventriglia_spermatogenesis_2025, title = {Spermatogenesis advancement in pre-pubertal meagre Argyrosomus regius treated with recombinant gonadotropins}, author = {Gianluca Ventriglia and Neil Duncan and Ignacio Giménez and Constantinos C Mylonas and Chrysovalentinos Pousis and Aldo Corriero and Rosa Zupa}, url = {https://www.nature.com/articles/s41598-025-99372-3 /wp-content/uploads/2025/05/2025-Ventriglia-SciRep-22.pdf}, doi = {10.1038/s41598-025-99372-3}, issn = {2045-2322}, year = {2025}, date = {2025-05-13}, urldate = {2025-05-13}, journal = {Scientific Reports}, volume = {15}, number = {1}, pages = {15113}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Papadaki, Maria; Le, Ngoc-Son; Mylonas, Constantinos C; Sarropoulou, Elena Marine Biotechnology, 27 (2), pp. 74, 2025, ISSN: 1436-2228, 1436-2236. @article{papadaki_exploring_2025, title = {Exploring the Fanconi Anemia Gene Expression and Regulation by MicroRNAs in Gilthead Seabream (Sparus aurata) at Different Gonadal Development Stages}, author = {Maria Papadaki and Ngoc-Son Le and Constantinos C Mylonas and Elena Sarropoulou}, url = {https://link.springer.com/10.1007/s10126-025-10444-x /wp-content/uploads/2025/05/2025-Papadaki-MarBioteck-21.pdf}, doi = {10.1007/s10126-025-10444-x}, issn = {1436-2228, 1436-2236}, year = {2025}, date = {2025-05-06}, urldate = {2025-05-06}, journal = {Marine Biotechnology}, volume = {27}, number = {2}, pages = {74}, abstract = {Abstract Fanconi anaemia (FA) is a rare autosomal recessive disease in humans that is distributed worldwide. Fanconi anemia complementation (Fanc) proteins are essential for the appropriate functioning of the FA DNA repair pathway. They are also linked to a number of other biological processes, including oxygen metabolism, cell cycle regulation, haematopoiesis and apoptosis. So far, little research has been conducted on teleosts, but evidence shows that Fanc proteins play a significant role in immune response and sex reversal. For the examination of the expression of three fanc genes ( fancc , fancl, and fancd2 ), as well as the potential regulation of these genes by microRNAs (miRNAs) in gonadal tissues at different stages of development, the present study has selected the gilthead seabream ( Sparus aurata ), a significant aquaculture species that exhibits protandrous hermaphroditism. The obtained data suggested the role of fancl and fancd2 in the maturation of female gonads and the miRNAs miR-210, miR-217 and miR-10926 have been identified as putative regulators of fancd2 , fancc and fancl , respectively. Overall, the data indicated the potential use of fancl and fancd2 genes as sex biomarkers in conjunction with their respective regulation by miRNAs. To the best of our knowledge, this is the first study demonstrating the importance of fanc genes, along with putative regulatory miRNAs, in the reproduction of an important marine aquaculture species.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Fanconi anaemia (FA) is a rare autosomal recessive disease in humans that is distributed worldwide. Fanconi anemia complementation (Fanc) proteins are essential for the appropriate functioning of the FA DNA repair pathway. They are also linked to a number of other biological processes, including oxygen metabolism, cell cycle regulation, haematopoiesis and apoptosis. So far, little research has been conducted on teleosts, but evidence shows that Fanc proteins play a significant role in immune response and sex reversal. For the examination of the expression of three fanc genes ( fancc , fancl, and fancd2 ), as well as the potential regulation of these genes by microRNAs (miRNAs) in gonadal tissues at different stages of development, the present study has selected the gilthead seabream ( Sparus aurata ), a significant aquaculture species that exhibits protandrous hermaphroditism. The obtained data suggested the role of fancl and fancd2 in the maturation of female gonads and the miRNAs miR-210, miR-217 and miR-10926 have been identified as putative regulators of fancd2 , fancc and fancl , respectively. Overall, the data indicated the potential use of fancl and fancd2 genes as sex biomarkers in conjunction with their respective regulation by miRNAs. To the best of our knowledge, this is the first study demonstrating the importance of fanc genes, along with putative regulatory miRNAs, in the reproduction of an important marine aquaculture species. |
Thieme, Philipp; Fischbach, Vivian; Papadakis, Ioannis; Moritz, Timo Development of convergent adaptations reveal highly conserved early ontogenetic skull shape in fishes with amphibious vision Journal Article Scientific Reports, 15 (1), pp. 8411, 2025, ISSN: 2045-2322. @article{thieme_development_2025, title = {Development of convergent adaptations reveal highly conserved early ontogenetic skull shape in fishes with amphibious vision}, author = {Philipp Thieme and Vivian Fischbach and Ioannis Papadakis and Timo Moritz}, url = {https://www.nature.com/articles/s41598-025-91563-2 /wp-content/uploads/2025/04/2025-Thieme-SR-20.pdf}, doi = {10.1038/s41598-025-91563-2}, issn = {2045-2322}, year = {2025}, date = {2025-04-28}, urldate = {2025-04-28}, journal = {Scientific Reports}, volume = {15}, number = {1}, pages = {8411}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Kapelonis, Zacharias; Chatzigeorgiou, Georgios; Ntoumas, Manolis; Grigoriou, Panos; Pettas, Manos; Michelinakis, Spyros; Correia, Ricardo; Lemos, Catarina Rasquilha; Pinheiro, Luis Menezes; Lomba, Caio; Fortuna, João; Loureiro, Rui; Santos, André; Chatzinikolaou, Eva Flying Robots Teach Floating Robots—A Machine Learning Approach for Marine Habitat Mapping Based on Combined Datasets Journal Article Journal of Marine Science and Engineering, 13 (3), pp. 611, 2025, ISSN: 2077-1312. @article{kapelonis_flying_2025, title = {Flying Robots Teach Floating Robots—A Machine Learning Approach for Marine Habitat Mapping Based on Combined Datasets}, author = {Zacharias Kapelonis and Georgios Chatzigeorgiou and Manolis Ntoumas and Panos Grigoriou and Manos Pettas and Spyros Michelinakis and Ricardo Correia and Catarina Rasquilha Lemos and Luis Menezes Pinheiro and Caio Lomba and João Fortuna and Rui Loureiro and André Santos and Eva Chatzinikolaou}, url = {https://www.mdpi.com/2077-1312/13/3/611 /wp-content/uploads/2025/04/2025-Kapelonis-Mar-Sci-Eng-19.pdf}, doi = {10.3390/jmse13030611}, issn = {2077-1312}, year = {2025}, date = {2025-04-11}, urldate = {2025-04-11}, journal = {Journal of Marine Science and Engineering}, volume = {13}, number = {3}, pages = {611}, abstract = {Unmanned aerial and autonomous surface vehicles (UAVs and ASVs, respectively) are two emerging technologies for the mapping of coastal and marine environments. Using UAV photogrammetry, the sea-bottom composition can be resolved with very high fidelity in shallow waters. At greater depths, acoustic methodologies have far better propagation properties compared to optics; therefore, ASVs equipped with multibeam echosounders (MBES) are better-suited for mapping applications in deeper waters. In this work, a sea-bottom classification methodology is presented for mapping the protected habitat of Mediterranean seagrass Posidonia oceanica (habitat code 1120) in a coastal subregion of Heraklion (Crete, Greece). The methodology implements a machine learning scheme, where knowledge obtained from UAV imagery is embedded (through training) into a classifier that utilizes acoustic backscatter intensity and features derived from the MBES data provided by an ASV. Accuracy and precision scores of greater than 85% compared with visual census ground-truth data for both optical and acoustic classifiers indicate that this hybrid mapping approach is promising to mitigate the depth-induced bias in UAV-only models. The latter is especially interesting in cases where the studied habitat boundaries extend beyond depths that can be studied via aerial devices’ optics, as is the case with P. oceanica meadows.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Unmanned aerial and autonomous surface vehicles (UAVs and ASVs, respectively) are two emerging technologies for the mapping of coastal and marine environments. Using UAV photogrammetry, the sea-bottom composition can be resolved with very high fidelity in shallow waters. At greater depths, acoustic methodologies have far better propagation properties compared to optics; therefore, ASVs equipped with multibeam echosounders (MBES) are better-suited for mapping applications in deeper waters. In this work, a sea-bottom classification methodology is presented for mapping the protected habitat of Mediterranean seagrass Posidonia oceanica (habitat code 1120) in a coastal subregion of Heraklion (Crete, Greece). The methodology implements a machine learning scheme, where knowledge obtained from UAV imagery is embedded (through training) into a classifier that utilizes acoustic backscatter intensity and features derived from the MBES data provided by an ASV. Accuracy and precision scores of greater than 85% compared with visual census ground-truth data for both optical and acoustic classifiers indicate that this hybrid mapping approach is promising to mitigate the depth-induced bias in UAV-only models. The latter is especially interesting in cases where the studied habitat boundaries extend beyond depths that can be studied via aerial devices’ optics, as is the case with P. oceanica meadows. |
Mukiibi, Robert; Ferraresso, Serena; Franch, Rafaella; Peruzza, Luca; Rovere, Giulia Dalla; Babbucci, Massimiliano; Bertotto, Daniela; Toffan, Anna; Pascoli, Francesco; Faggion, Sara; Peñaloza, Carolina; Tsigenopoulos, Costas S; Houston, Ross D; Bargelloni, Luca; Robledo, Diego Integrated functional genomic analysis identifies regulatory variants underlying a major QTL for disease resistance in European sea bass Journal Article BMC Biology, 23 (1), pp. 75, 2025, ISSN: 1741-7007. @article{mukiibi_integrated_2025, title = {Integrated functional genomic analysis identifies regulatory variants underlying a major QTL for disease resistance in European sea bass}, author = {Robert Mukiibi and Serena Ferraresso and Rafaella Franch and Luca Peruzza and Giulia Dalla Rovere and Massimiliano Babbucci and Daniela Bertotto and Anna Toffan and Francesco Pascoli and Sara Faggion and Carolina Peñaloza and Costas S Tsigenopoulos and Ross D Houston and Luca Bargelloni and Diego Robledo}, url = {https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-025-02180-4 /wp-content/uploads/2025/04/2025-Mukiibi-BMC-Biology-18.pdf}, doi = {10.1186/s12915-025-02180-4}, issn = {1741-7007}, year = {2025}, date = {2025-04-07}, urldate = {2025-04-07}, journal = {BMC Biology}, volume = {23}, number = {1}, pages = {75}, abstract = {Abstract Background Viral nervous necrosis (VNN) is an important viral disease threatening global aquaculture sustainability and affecting over 50 farmed and ecologically important fish species. A major QTL for resistance to VNN has been previously detected in European sea bass, but the underlying causal gene(s) and mutation(s) remain unknown. To identify the mechanisms and genetic factors underpinning resistance to VNN in European sea bass, we employed integrative analyses of multiple functional genomics assays in European sea bass. Results The estimated heritability of VNN resistance was high ( h 2 textasciitilde 0.40), and a major QTL explaining up to 38% of the genetic variance of the trait was confirmed on chromosome 3, with individuals with the resistant QTL genotype showing a 90% survivability against a VNN outbreak. Whole-genome resequencing analyses narrowed the location of this QTL to a small region containing 4 copies of interferon alpha inducible protein 27-like 2A ( IFI27L2A ) genes, and one copy of the interferon alpha inducible protein 27-like 2 ( IFI27L2 ) gene. RNA sequencing revealed a clear association between the QTL genotype and the expression of two of the IFI27L2A genes, and the IFI27L2 gene. Integration with chromatin accessibility and histone modification data pinpointed two SNPs in active regulatory regions of two of these genes ( IFI27L2A and IFI27L2 ), and transcription factor binding site gains for the resistant alleles were predicted. These alleles, particularly the SNP variant CHR3:10,077,301, exhibited higher frequencies (0.55 to 0.77) in Eastern Mediterranean Sea bass populations, which show considerably higher levels of resistance to VNN, as compared to susceptible West Mediterranean and Atlantic populations (0.15–0.25). Conclusions The SNP variant CHR3:10,077,301, through modulation of IFI27L2 and IFI27L2A genes, is likely the causative mutation underlying resistance to VNN in European sea bass. This is one of the first causative mutations discovered for disease resistance traits in fish and paves the way for marker-assisted selection as well as biotechnological approaches to enhance resistance to VNN in European sea bass and other susceptible species.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Background Viral nervous necrosis (VNN) is an important viral disease threatening global aquaculture sustainability and affecting over 50 farmed and ecologically important fish species. A major QTL for resistance to VNN has been previously detected in European sea bass, but the underlying causal gene(s) and mutation(s) remain unknown. To identify the mechanisms and genetic factors underpinning resistance to VNN in European sea bass, we employed integrative analyses of multiple functional genomics assays in European sea bass. Results The estimated heritability of VNN resistance was high ( h 2 textasciitilde 0.40), and a major QTL explaining up to 38% of the genetic variance of the trait was confirmed on chromosome 3, with individuals with the resistant QTL genotype showing a 90% survivability against a VNN outbreak. Whole-genome resequencing analyses narrowed the location of this QTL to a small region containing 4 copies of interferon alpha inducible protein 27-like 2A ( IFI27L2A ) genes, and one copy of the interferon alpha inducible protein 27-like 2 ( IFI27L2 ) gene. RNA sequencing revealed a clear association between the QTL genotype and the expression of two of the IFI27L2A genes, and the IFI27L2 gene. Integration with chromatin accessibility and histone modification data pinpointed two SNPs in active regulatory regions of two of these genes ( IFI27L2A and IFI27L2 ), and transcription factor binding site gains for the resistant alleles were predicted. These alleles, particularly the SNP variant CHR3:10,077,301, exhibited higher frequencies (0.55 to 0.77) in Eastern Mediterranean Sea bass populations, which show considerably higher levels of resistance to VNN, as compared to susceptible West Mediterranean and Atlantic populations (0.15–0.25). Conclusions The SNP variant CHR3:10,077,301, through modulation of IFI27L2 and IFI27L2A genes, is likely the causative mutation underlying resistance to VNN in European sea bass. This is one of the first causative mutations discovered for disease resistance traits in fish and paves the way for marker-assisted selection as well as biotechnological approaches to enhance resistance to VNN in European sea bass and other susceptible species. |
Wei, Xiao-Lei; Hao, Zhi-Wei; Kotzamanis, Yannis P; Zhang, Tian-Hua; Liu, Zhi-Bo; Yang, Hong; Luo, Zhi Aquaculture Reports, 42 , pp. 102739, 2025, ISSN: 23525134. @article{wei_dietary_2025, title = {Dietary iron oxide (Fe2O3) nanoparticles modulate growth performance, body composition, mineral content and intestinal health of yellow catfish juveniles (Pelteobagrus fulvidraco)}, author = {Xiao-Lei Wei and Zhi-Wei Hao and Yannis P Kotzamanis and Tian-Hua Zhang and Zhi-Bo Liu and Hong Yang and Zhi Luo}, url = {https://linkinghub.elsevier.com/retrieve/pii/S2352513425001255 /wp-content/uploads/2025/03/2025-Wei-AquaReports-16.pdf}, doi = {10.1016/j.aqrep.2025.102739}, issn = {23525134}, year = {2025}, date = {2025-03-31}, urldate = {2025-03-31}, journal = {Aquaculture Reports}, volume = {42}, pages = {102739}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Antoniou, Theodora; Dimitriou, Andreas C; Karameta, Emmanouela; Antoniou, Aglaia; Poulakakis, Nikos; Sfenthourakis, Spyros Comparative phylogeography of four lizard taxa within an oceanic island Journal Article Molecular Phylogenetics and Evolution, 205 , pp. 108295, 2025, ISSN: 10557903. @article{antoniou_comparative_2025, title = {Comparative phylogeography of four lizard taxa within an oceanic island}, author = {Theodora Antoniou and Andreas C Dimitriou and Emmanouela Karameta and Aglaia Antoniou and Nikos Poulakakis and Spyros Sfenthourakis}, url = {https://linkinghub.elsevier.com/retrieve/pii/S1055790325000120 /wp-content/uploads/2025/03/2025-Antoniou-CompPhylo-pre-print-15.pdf}, doi = {10.1016/j.ympev.2025.108295}, issn = {10557903}, year = {2025}, date = {2025-03-24}, urldate = {2025-03-24}, journal = {Molecular Phylogenetics and Evolution}, volume = {205}, pages = {108295}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Droubogiannis, Stavros; Katharios, Pantelis Phage Therapy in Aquaculture Incollection Elumalai, Preetham; Lakshmi, Sreeja (Ed.): Antimicrobial Resistance in Aquaculture and Aquatic Environments, pp. 229–255, Springer Nature Singapore, Singapore, 2025, ISBN: 9789819773190 9789819773206. @incollection{elumalai_phage_2025, title = {Phage Therapy in Aquaculture}, author = {Stavros Droubogiannis and Pantelis Katharios}, editor = {Preetham Elumalai and Sreeja Lakshmi}, url = {https://link.springer.com/10.1007/978-981-97-7320-6_10 /wp-content/uploads/2025/03/2025-Droumbogiannis-and-Katharios-phage-therapy-in-aquaculture-pre-print-14.pdf}, doi = {10.1007/978-981-97-7320-6_10}, isbn = {9789819773190 9789819773206}, year = {2025}, date = {2025-03-20}, urldate = {2025-03-20}, booktitle = {Antimicrobial Resistance in Aquaculture and Aquatic Environments}, pages = {229--255}, publisher = {Springer Nature Singapore}, address = {Singapore}, keywords = {}, pubstate = {published}, tppubtype = {incollection} } |
Chalmoukis, Charalampos; Droubogiannis, Stavros; Michalopoulou, Vassiliki A; Triga, Adriana; Sarris, Panagiotis F; Katharios, Pantelis Pathogens, 14 (3), pp. 247, 2025, ISSN: 2076-0817. @article{chalmoukis_development_2025, title = {Development and Characterization of Fluorescent Protein-Tagged Vibrio harveyi Strains as a Versatile Tool for Studying Infection Dynamics and Strain Interactions}, author = {Charalampos Chalmoukis and Stavros Droubogiannis and Vassiliki A Michalopoulou and Adriana Triga and Panagiotis F Sarris and Pantelis Katharios}, url = {https://www.mdpi.com/2076-0817/14/3/247 /wp-content/uploads/2025/03/2025-Chalmoukis-pathogens-13.pdf}, doi = {10.3390/pathogens14030247}, issn = {2076-0817}, year = {2025}, date = {2025-03-18}, urldate = {2025-03-18}, journal = {Pathogens}, volume = {14}, number = {3}, pages = {247}, abstract = {Fluorescent protein-tagged bacterial strains are widely used tools for studying host-pathogen interactions and microbial dynamics. In this study, we developed and characterized Vibrio harveyi strains genetically modified to express green fluorescent protein (GFP) and red fluorescent protein (RFP). These strains were constructed using triparental mating and evaluated for phenotypic, genomic, and virulence attributes. Genomic analyses revealed strain-specific variations, including mutations in key regulatory and metabolic genes, such as luxO and transketolase. While plasmid acquisition imposed metabolic costs, resulting in altered growth and antibiotic sensitivities in certain transconjugants, others demonstrated robust phenotypic stability. Virulence assays using gilthead seabream larvae revealed that most tagged strains retained moderate pathogenicity, with visualization of co-infections highlighting the potential for studying strain-specific interactions. Furthermore, fluorescent microscopy confirmed the successful colonization and localization of tagged bacteria within host tissues. These findings underscore the utility of GFP- and RFP-tagged Vibrio harveyi as versatile tools for infection dynamics, offering a foundation for future research on strain interactions and pathogen-host relationships.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Fluorescent protein-tagged bacterial strains are widely used tools for studying host-pathogen interactions and microbial dynamics. In this study, we developed and characterized Vibrio harveyi strains genetically modified to express green fluorescent protein (GFP) and red fluorescent protein (RFP). These strains were constructed using triparental mating and evaluated for phenotypic, genomic, and virulence attributes. Genomic analyses revealed strain-specific variations, including mutations in key regulatory and metabolic genes, such as luxO and transketolase. While plasmid acquisition imposed metabolic costs, resulting in altered growth and antibiotic sensitivities in certain transconjugants, others demonstrated robust phenotypic stability. Virulence assays using gilthead seabream larvae revealed that most tagged strains retained moderate pathogenicity, with visualization of co-infections highlighting the potential for studying strain-specific interactions. Furthermore, fluorescent microscopy confirmed the successful colonization and localization of tagged bacteria within host tissues. These findings underscore the utility of GFP- and RFP-tagged Vibrio harveyi as versatile tools for infection dynamics, offering a foundation for future research on strain interactions and pathogen-host relationships. |
Costanzo, Di F; Marsico, Di M; Orefice, I; Kristoffersen, J B; Kasapidis, P; Chaumier, T; Ambrosino, L; Miralto, M; Cigliano, Aiese R; Verret, F; Tirichine, L; Trindade, M; Zyl, Van L; Dato, Di V; Romano, G High-quality genome assembly and annotation of Thalassiosira rotula (synonym of Thalassiosira gravida) Journal Article Scientific Data, 12 (1), pp. 310, 2025, ISSN: 2052-4463. @article{di_costanzo_high-quality_2025, title = {High-quality genome assembly and annotation of Thalassiosira rotula (synonym of Thalassiosira gravida)}, author = {F Di Costanzo and M Di Marsico and I Orefice and J B Kristoffersen and P Kasapidis and T Chaumier and L Ambrosino and M Miralto and R Aiese Cigliano and F Verret and L Tirichine and M Trindade and L Van Zyl and V Di Dato and G Romano}, url = {https://www.nature.com/articles/s41597-025-04634-4 /wp-content/uploads/2025/03/2025-Costanzo-SciData-12.pdf}, doi = {10.1038/s41597-025-04634-4}, issn = {2052-4463}, year = {2025}, date = {2025-03-14}, urldate = {2025-03-14}, journal = {Scientific Data}, volume = {12}, number = {1}, pages = {310}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Sarropoulou, Elena; Katharios, Pantelis; Kaitetzidou, Elisavet; Scapigliati, Giuseppe; Miccoli, Andrea Circulating miRNAs involved in the immune response of the European seabass (Dicentrarchus labrax) Journal Article Fish & Shellfish Immunology, 160 , pp. 110232, 2025, ISSN: 10504648. @article{sarropoulou_circulating_2025, title = {Circulating miRNAs involved in the immune response of the European seabass (Dicentrarchus labrax)}, author = {Elena Sarropoulou and Pantelis Katharios and Elisavet Kaitetzidou and Giuseppe Scapigliati and Andrea Miccoli}, url = {https://linkinghub.elsevier.com/retrieve/pii/S1050464825001214 /wp-content/uploads/2025/03/2025-Sarropoulou-FishShelImm-11.pdf}, doi = {10.1016/j.fsi.2025.110232}, issn = {10504648}, year = {2025}, date = {2025-03-10}, urldate = {2025-03-11}, journal = {Fish & Shellfish Immunology}, volume = {160}, pages = {110232}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Katharios, Pantelis; Karageorgou, Evangelia Protozoan Co‐Infection Drives Fish Mortality Event in Crete's Karteros River Journal Article Journal of Fish Diseases, pp. e14099, 2025, ISSN: 0140-7775, 1365-2761. @article{katharios_protozoan_2025, title = {Protozoan Co‐Infection Drives Fish Mortality Event in Crete's Karteros River}, author = {Pantelis Katharios and Evangelia Karageorgou}, url = {https://onlinelibrary.wiley.com/doi/10.1111/jfd.14099 /wp-content/uploads/2025/03/2025-Katharios-JFD-10.pdf}, doi = {10.1111/jfd.14099}, issn = {0140-7775, 1365-2761}, year = {2025}, date = {2025-03-07}, urldate = {2025-03-10}, journal = {Journal of Fish Diseases}, pages = {e14099}, abstract = {ABSTRACT In August 2024, a significant fish kill involving hundreds of flathead grey mullet (\textit{Mugil cephalus} ) was reported in the delta region of the Karteros River, Crete, Greece. The investigation identified the primary cause of mortality as severe parasitic infections, specifically from the protozoan parasites Amyloodinium ocellatum and Trichodina sp., both of which heavily affected the gills of the fish. Concurrently, poor water quality in the area, likely due to reduced water volume and limited water renewal, created favourable conditions for parasite proliferation while weakening the fish's natural defences. This event raised concerns due to the location of the fish kill in the Karteros River delta, a highly protected area designated as a small island wetland.}, keywords = {}, pubstate = {published}, tppubtype = {article} } ABSTRACT In August 2024, a significant fish kill involving hundreds of flathead grey mullet (Mugil cephalus ) was reported in the delta region of the Karteros River, Crete, Greece. The investigation identified the primary cause of mortality as severe parasitic infections, specifically from the protozoan parasites Amyloodinium ocellatum and Trichodina sp., both of which heavily affected the gills of the fish. Concurrently, poor water quality in the area, likely due to reduced water volume and limited water renewal, created favourable conditions for parasite proliferation while weakening the fish's natural defences. This event raised concerns due to the location of the fish kill in the Karteros River delta, a highly protected area designated as a small island wetland. |
Brecko, Jonathan; Mathys, Aurore; Chatzinikolaou, Eva; Keklikoglou, Kleoniki; Blettery, Jonathan; Green, Laura; Musson, Alicia; Paton, Alan; Phillips, Sarah; Bastir, Markus; Wiltschke, Karin; Rainer, Heimo; Kroh, Andreas; Haston, Elspeth; Semal, Patrick DIGIT-KEY: an aid towards uniform 2D+ and 3D digitisation techniques within natural history collections Journal Article European Journal of Taxonomy, 976 , 2025, ISSN: 2118-9773. @article{brecko_digit-key_2025, title = {DIGIT-KEY: an aid towards uniform 2D+ and 3D digitisation techniques within natural history collections}, author = {Jonathan Brecko and Aurore Mathys and Eva Chatzinikolaou and Kleoniki Keklikoglou and Jonathan Blettery and Laura Green and Alicia Musson and Alan Paton and Sarah Phillips and Markus Bastir and Karin Wiltschke and Heimo Rainer and Andreas Kroh and Elspeth Haston and Patrick Semal}, url = {https://europeanjournaloftaxonomy.eu/index.php/ejt/article/view/2797 /wp-content/uploads/2025/03/2025-Brecko-EuJTax-9.pdf}, doi = {10.5852/ejt.2025.976.2797}, issn = {2118-9773}, year = {2025}, date = {2025-03-04}, urldate = {2025-03-04}, journal = {European Journal of Taxonomy}, volume = {976}, abstract = {Natural History institutes hold an immense number of specimens and artefacts. For years these collections were not accessible online, remaining inaccessible to researchers from far away and hidden from the general public. Large digitisation projects and cross-institutional agreements aim to bring their collections into the digital era, such as the SYNTHESYS+ project and the Distributed System of Scientific Collections (DiSSCo) Research Infrastructure. As specimens are 3D physical objects with different characteristics many techniques are available to 3D digitise them. For inexperienced users this can be quite overwhelming. Which techniques are already well tested in other institutions and are suitable for a specific specimen or collection? To investigate this, we have set up a dichotomous identification key for digitisation techniques: DIGIT-KEY, (https://digit.naturalheritage.be/digit-key). For each technique, examples used in SYNTHESYS+ Institutions are visualised and training manuals provided. All information can be easily updated and representatives can be contacted if necessary to request more information about a certain technique. This key can be helpful to achieve comparable results across institutions when digitising collections on demand in future DiSSCo research initiatives coordinated through the European Loans and Visits System (ELViS) for Virtual and Transnational Access.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Natural History institutes hold an immense number of specimens and artefacts. For years these collections were not accessible online, remaining inaccessible to researchers from far away and hidden from the general public. Large digitisation projects and cross-institutional agreements aim to bring their collections into the digital era, such as the SYNTHESYS+ project and the Distributed System of Scientific Collections (DiSSCo) Research Infrastructure. As specimens are 3D physical objects with different characteristics many techniques are available to 3D digitise them. For inexperienced users this can be quite overwhelming. Which techniques are already well tested in other institutions and are suitable for a specific specimen or collection? To investigate this, we have set up a dichotomous identification key for digitisation techniques: DIGIT-KEY, (https://digit.naturalheritage.be/digit-key). For each technique, examples used in SYNTHESYS+ Institutions are visualised and training manuals provided. All information can be easily updated and representatives can be contacted if necessary to request more information about a certain technique. This key can be helpful to achieve comparable results across institutions when digitising collections on demand in future DiSSCo research initiatives coordinated through the European Loans and Visits System (ELViS) for Virtual and Transnational Access. |
Grigorakis, Kriton; Kogiannou, Dimitra; Kotsiri, Mado; Kleidas, Ioannis; Mello, Paulo De H; Habiballah, Salaheldeen; Alshaikhi, Ali; Alhafedh, Youssef S; Mohamed, Asaad H W Freshness and Spoilage Patterns of Wild and Farmed Tropical Fish Species with Major Commercial Importance Originating from Saudi Arabian Waters Journal Article Foods, 14 (4), pp. 690, 2025, ISSN: 2304-8158. @article{grigorakis_freshness_2025, title = {Freshness and Spoilage Patterns of Wild and Farmed Tropical Fish Species with Major Commercial Importance Originating from Saudi Arabian Waters}, author = {Kriton Grigorakis and Dimitra Kogiannou and Mado Kotsiri and Ioannis Kleidas and Paulo H De Mello and Salaheldeen Habiballah and Ali Alshaikhi and Youssef S Alhafedh and Asaad H W Mohamed}, url = {https://www.mdpi.com/2304-8158/14/4/690 /wp-content/uploads/2025/02/2025-Grigorakis-foods-8.pdf}, doi = {10.3390/foods14040690}, issn = {2304-8158}, year = {2025}, date = {2025-02-24}, urldate = {2025-02-24}, journal = {Foods}, volume = {14}, number = {4}, pages = {690}, abstract = {Ice-stored farmed barramundi (Lates calcarifer), snubnose pompano (Trachinotus blochii) and sobaity bream (Sparidentex hasta), as well as wild-caught cobia (Rachycentron canadum), coral trout (Plectropomus leopardus), giant trevally (Caranx ignobilis), milkfish (Chanos chanos) and mangrove red snapper (Lutjanus argentimaculatus), were compared for their freshness/spoilage using sensory, chemical and microbiological methods. Quality Index Method schemes were developed to determine alterations in the sensory freshness. The shelf lives ranged from 8 (coral trout) to 18 days (sobaity bream). The farmed species always exhibited a significantly longer shelf life than the wild-caught species. The adenosine triphosphate (ATP) breakdown followed different patterns in the studied species. The K-values at the time of sensory rejection ranged from 30 to 80% depending on the species, while the microbial load reached or exceeded a level of 6 log cfu/g. Although the shelf life duration was dependent on the origin of the fish (wild or farmed), the ATP breakdown scheme, as well as the K-values and microbial loads at the time of rejection, were species-dependent and independent of the origin.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Ice-stored farmed barramundi (Lates calcarifer), snubnose pompano (Trachinotus blochii) and sobaity bream (Sparidentex hasta), as well as wild-caught cobia (Rachycentron canadum), coral trout (Plectropomus leopardus), giant trevally (Caranx ignobilis), milkfish (Chanos chanos) and mangrove red snapper (Lutjanus argentimaculatus), were compared for their freshness/spoilage using sensory, chemical and microbiological methods. Quality Index Method schemes were developed to determine alterations in the sensory freshness. The shelf lives ranged from 8 (coral trout) to 18 days (sobaity bream). The farmed species always exhibited a significantly longer shelf life than the wild-caught species. The adenosine triphosphate (ATP) breakdown followed different patterns in the studied species. The K-values at the time of sensory rejection ranged from 30 to 80% depending on the species, while the microbial load reached or exceeded a level of 6 log cfu/g. Although the shelf life duration was dependent on the origin of the fish (wild or farmed), the ATP breakdown scheme, as well as the K-values and microbial loads at the time of rejection, were species-dependent and independent of the origin. |
Carlot, J; Galobart, C; Gómez-Gras, D; Santamaría, J; Golo, R; Sini, M; Cebrian, E; Gerovasileiou, V; Ponti, M; Turicchia, E; Comeau, S; Rilov, G; Tamburello, L; Mantas, Pulido T; Cerrano, C; Ledoux, J B; Gattuso, J -P; Ramirez-Calero, S; Millan, L; Montefalcone, M; Katsanevakis, S; Bensoussan, N; Garrabou, J; Teixidó, N Vulnerability of benthic trait diversity across the Mediterranean Sea following mass mortality events Journal Article Nature Communications, 16 (1), pp. 1571, 2025, ISSN: 2041-1723. @article{carlot_vulnerability_2025, title = {Vulnerability of benthic trait diversity across the Mediterranean Sea following mass mortality events}, author = {J Carlot and C Galobart and D Gómez-Gras and J Santamaría and R Golo and M Sini and E Cebrian and V Gerovasileiou and M Ponti and E Turicchia and S Comeau and G Rilov and L Tamburello and T Pulido Mantas and C Cerrano and J B Ledoux and J -P Gattuso and S Ramirez-Calero and L Millan and M Montefalcone and S Katsanevakis and N Bensoussan and J Garrabou and N Teixidó}, url = {https://www.nature.com/articles/s41467-025-55949-0 /wp-content/uploads/2025/02/2025-Carlot-Nat-Comm-7.pdf}, doi = {10.1038/s41467-025-55949-0}, issn = {2041-1723}, year = {2025}, date = {2025-02-21}, urldate = {2025-02-20}, journal = {Nature Communications}, volume = {16}, number = {1}, pages = {1571}, abstract = {Abstract Unraveling the functional future of marine ecosystems amid global change poses a pressing challenge. This is particularly critical in the Mediterranean Sea, which is highly impacted by global and local drivers. Utilizing extensive mass mortality events (MMEs) datasets spanning from 1986 to 2020 across the Mediterranean Sea, we investigated the trait vulnerability of benthic species that suffered from MMEs induced by nine distinct mortality drivers. By analyzing changes in ten ecological traits across 389 benthic species—constituting an extensive compendium of Mediterranean ecological traits to date—we identified 228 functional entities (FEs), defined as groups of species sharing the same trait values. Our findings indicate that of these 55 FEs were impacted by MMEs, accentuating a heightened vulnerability within specific trait categories. Notably, more than half of the mortality records showed severe impacts on calcifying and larger species with slower growth which mostly account for tree-like and massive forms. Altogether, we highlight that 29 FEs suffered extreme mortality, leading to a maximum increase of 19.1% of the global trait volume vulnerability over 35 years. We also reveal that 10.8% of the trait volume may have been temporarily lost over the last five years, emphasizing the risk of a rapid ecological transformation in the Mediterranean Sea.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Unraveling the functional future of marine ecosystems amid global change poses a pressing challenge. This is particularly critical in the Mediterranean Sea, which is highly impacted by global and local drivers. Utilizing extensive mass mortality events (MMEs) datasets spanning from 1986 to 2020 across the Mediterranean Sea, we investigated the trait vulnerability of benthic species that suffered from MMEs induced by nine distinct mortality drivers. By analyzing changes in ten ecological traits across 389 benthic species—constituting an extensive compendium of Mediterranean ecological traits to date—we identified 228 functional entities (FEs), defined as groups of species sharing the same trait values. Our findings indicate that of these 55 FEs were impacted by MMEs, accentuating a heightened vulnerability within specific trait categories. Notably, more than half of the mortality records showed severe impacts on calcifying and larger species with slower growth which mostly account for tree-like and massive forms. Altogether, we highlight that 29 FEs suffered extreme mortality, leading to a maximum increase of 19.1% of the global trait volume vulnerability over 35 years. We also reveal that 10.8% of the trait volume may have been temporarily lost over the last five years, emphasizing the risk of a rapid ecological transformation in the Mediterranean Sea. |
Daraghmeh, Nauras; Exter, Katrina; Pagnier, Justine; Balazy, Piotr; Cancio, Ibon; Chatzigeorgiou, Giorgos; Chatzinikolaou, Eva; Chelchowski, Maciej; Chrismas, Nathan Alexis Mitchell; Comtet, Thierry; Dailianis, Thanos; Deneudt, Klaas; Cerio, Oihane Diaz De; Digenis, Markos; Gerovasileiou, Vasilis; González, José; Kauppi, Laura; Kristoffersen, Jon Bent; Kukliński, Piotr; Lasota, Rafał; Levy, Liraz; Małachowicz, Magdalena; Mavrič, Borut; Mortelmans, Jonas; Paredes, Estefania; Poćwierz‐Kotus, Anita; Reiss, Henning; Santi, Ioulia; Sarafidou, Georgia; Skouradakis, Grigorios; Solbakken, Jostein; Staehr, Peter A U; Tajadura, Javier; Thyrring, Jakob; Troncoso, Jesus S; Vernadou, Emmanouela; Viard, Frederique; Zafeiropoulos, Haris; Zbawicka, Małgorzata; Pavloudi, Christina; Obst, Matthias A Long-Term Ecological Research Data Set From the Marine Genetic Monitoring Program ARMS-MBON 2018–2020 Journal Article Molecular Ecology Resources, pp. e14073, 2025, ISSN: 1755-098X, 1755-0998. @article{daraghmeh_longterm_2025, title = {A Long-Term Ecological Research Data Set From the Marine Genetic Monitoring Program ARMS-MBON 2018–2020}, author = {Nauras Daraghmeh and Katrina Exter and Justine Pagnier and Piotr Balazy and Ibon Cancio and Giorgos Chatzigeorgiou and Eva Chatzinikolaou and Maciej Chelchowski and Nathan Alexis Mitchell Chrismas and Thierry Comtet and Thanos Dailianis and Klaas Deneudt and Oihane Diaz De Cerio and Markos Digenis and Vasilis Gerovasileiou and José González and Laura Kauppi and Jon Bent Kristoffersen and Piotr Kukliński and Rafał Lasota and Liraz Levy and Magdalena Małachowicz and Borut Mavrič and Jonas Mortelmans and Estefania Paredes and Anita Poćwierz‐Kotus and Henning Reiss and Ioulia Santi and Georgia Sarafidou and Grigorios Skouradakis and Jostein Solbakken and Peter A U Staehr and Javier Tajadura and Jakob Thyrring and Jesus S Troncoso and Emmanouela Vernadou and Frederique Viard and Haris Zafeiropoulos and Małgorzata Zbawicka and Christina Pavloudi and Matthias Obst}, url = {https://onlinelibrary.wiley.com/doi/10.1111/1755-0998.14073 /wp-content/uploads/2025/02/2025-Daraghmeh-MolEcoRes-6.pdf}, doi = {10.1111/1755-0998.14073}, issn = {1755-098X, 1755-0998}, year = {2025}, date = {2025-02-05}, urldate = {2025-02-05}, journal = {Molecular Ecology Resources}, pages = {e14073}, abstract = {ABSTRACT Molecular methods such as DNA/eDNA metabarcoding have emerged as useful tools to document the biodiversity of complex communities over large spatio‐temporal scales. We established an international Marine Biodiversity Observation Network (ARMS‐MBON) combining standardised sampling using autonomous reef monitoring structures (ARMS) with metabarcoding for genetic monitoring of marine hard‐bottom benthic communities. Here, we present the data of our first sampling campaign comprising 56 ARMS units deployed in 2018–2019 and retrieved in 2018–2020 across 15 observatories along the coasts of Europe and adjacent regions. We describe the open‐access data set (image, genetic and metadata) and explore the genetic data to show its potential for marine biodiversity monitoring and ecological research. Our analysis shows that ARMS recovered more than 60 eukaryotic phyla capturing diversity of up to textasciitilde5500 amplicon sequence variants and textasciitilde1800 operational taxonomic units, and up to textasciitilde250 and textasciitilde50 species per observatory using the cytochrome c oxidase subunit I (COI) and 18S rRNA marker genes, respectively. Further, ARMS detected threatened, vulnerable and non‐indigenous species often targeted in biological monitoring. We show that while deployment duration does not drive diversity estimates, sampling effort and sequencing depth across observatories do. We recommend that ARMS should be deployed for at least 3–6 months during the main growth season to use resources as efficiently as possible and that post‐sequencing curation is applied to enable statistical comparison of spatio‐temporal entities. We suggest that ARMS should be used in biological monitoring programs and long‐term ecological research and encourage the adoption of our ARMS‐MBON protocols.}, keywords = {}, pubstate = {published}, tppubtype = {article} } ABSTRACT Molecular methods such as DNA/eDNA metabarcoding have emerged as useful tools to document the biodiversity of complex communities over large spatio‐temporal scales. We established an international Marine Biodiversity Observation Network (ARMS‐MBON) combining standardised sampling using autonomous reef monitoring structures (ARMS) with metabarcoding for genetic monitoring of marine hard‐bottom benthic communities. Here, we present the data of our first sampling campaign comprising 56 ARMS units deployed in 2018–2019 and retrieved in 2018–2020 across 15 observatories along the coasts of Europe and adjacent regions. We describe the open‐access data set (image, genetic and metadata) and explore the genetic data to show its potential for marine biodiversity monitoring and ecological research. Our analysis shows that ARMS recovered more than 60 eukaryotic phyla capturing diversity of up to textasciitilde5500 amplicon sequence variants and textasciitilde1800 operational taxonomic units, and up to textasciitilde250 and textasciitilde50 species per observatory using the cytochrome c oxidase subunit I (COI) and 18S rRNA marker genes, respectively. Further, ARMS detected threatened, vulnerable and non‐indigenous species often targeted in biological monitoring. We show that while deployment duration does not drive diversity estimates, sampling effort and sequencing depth across observatories do. We recommend that ARMS should be deployed for at least 3–6 months during the main growth season to use resources as efficiently as possible and that post‐sequencing curation is applied to enable statistical comparison of spatio‐temporal entities. We suggest that ARMS should be used in biological monitoring programs and long‐term ecological research and encourage the adoption of our ARMS‐MBON protocols. |
Chen, I-Hao; Georgopoulou, Dimitra G; Ebbesson, Lars O E; Voskakis, Dimitris; Munthe-Kaas, Antonella Zanna; Papandroulakis, Nikos Acoustic tags versus camera—a case study on feeding behaviour of European seabass in sea cages Journal Article Frontiers in Marine Science, 11 , pp. 1497336, 2025, ISSN: 2296-7745. @article{chen_acoustic_2025, title = {Acoustic tags versus camera—a case study on feeding behaviour of European seabass in sea cages}, author = {I-Hao Chen and Dimitra G Georgopoulou and Lars O E Ebbesson and Dimitris Voskakis and Antonella Zanna Munthe-Kaas and Nikos Papandroulakis}, url = {https://www.frontiersin.org/articles/10.3389/fmars.2024.1497336/full https://imbbc.hcmr.gr/wp-content/uploads/2025/01/2025-Chen-fmars-5.pdf}, doi = {10.3389/fmars.2024.1497336}, issn = {2296-7745}, year = {2025}, date = {2025-01-30}, urldate = {2025-01-30}, journal = {Frontiers in Marine Science}, volume = {11}, pages = {1497336}, abstract = {Intoduction With the expansion of the aquaculture industry, the need arises for scalable, reliable, and robust methods to assess fish behaviour in sea cages to guide operational management, which includes feeding optimisation and welfare assessments. Fish cage monitoring utilising either acoustic transmitters or underwater cameras is well-studied. However, the relationship between those two different measurement types seems to have not been explored, nor have they been evaluated together in one experimental site. Methods In our 1-month study, we compared the activity of 14 sentinel fish and the artificial intelligence (AI)-inferred speed of individuals from the European seabass ( Dicentrarchus labrax ) sea cage population in three feeding trials. Comparisons include a maximum activity comparison using persistent peaks, fish behavioural pattern establishment and retention, and periodical behavioural patterns. Results Our results demonstrate that under certain circumstances, both technologies are interchangeable from the perspective of persistent peaks and periodicity, but complementary when it comes to behaviour analysis such as food anticipatory behaviour (FAB). Discussion We anticipate that our findings will stimulate advances where multiple sensor types are in use to achieve a more holistic understanding of fish behaviour in the aquaculture sector using underwater technologies.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Intoduction With the expansion of the aquaculture industry, the need arises for scalable, reliable, and robust methods to assess fish behaviour in sea cages to guide operational management, which includes feeding optimisation and welfare assessments. Fish cage monitoring utilising either acoustic transmitters or underwater cameras is well-studied. However, the relationship between those two different measurement types seems to have not been explored, nor have they been evaluated together in one experimental site. Methods In our 1-month study, we compared the activity of 14 sentinel fish and the artificial intelligence (AI)-inferred speed of individuals from the European seabass ( Dicentrarchus labrax ) sea cage population in three feeding trials. Comparisons include a maximum activity comparison using persistent peaks, fish behavioural pattern establishment and retention, and periodical behavioural patterns. Results Our results demonstrate that under certain circumstances, both technologies are interchangeable from the perspective of persistent peaks and periodicity, but complementary when it comes to behaviour analysis such as food anticipatory behaviour (FAB). Discussion We anticipate that our findings will stimulate advances where multiple sensor types are in use to achieve a more holistic understanding of fish behaviour in the aquaculture sector using underwater technologies. |
Oikonomou, Stavroula; Tasiouli, Katerina; Tsaparis, Dimitrios; Manousaki, Tereza; Vallecillos, Antonio; Oikonomaki, Katerina; Tzokas, Konstantinos; Katribouzas, Nikolaos; Batargias, Costas; Chatziplis, Dimitrios; Tsigenopoulos, Costas S Genomic evaluation for body weight, length and growth estimates in meagre Argyrosomus regius Journal Article Aquaculture, 595 , pp. 741622, 2025, ISSN: 00448486. @article{oikonomou_genomic_2025, title = {Genomic evaluation for body weight, length and growth estimates in meagre Argyrosomus regius}, author = {Stavroula Oikonomou and Katerina Tasiouli and Dimitrios Tsaparis and Tereza Manousaki and Antonio Vallecillos and Katerina Oikonomaki and Konstantinos Tzokas and Nikolaos Katribouzas and Costas Batargias and Dimitrios Chatziplis and Costas S Tsigenopoulos}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848624010846 /wp-content/uploads/2025/01/2025-Oikonomou-AQUA-3.pdf}, doi = {10.1016/j.aquaculture.2024.741622}, issn = {00448486}, year = {2025}, date = {2025-01-24}, urldate = {2025-01-24}, journal = {Aquaculture}, volume = {595}, pages = {741622}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Lancerotto, Stefano; Fakriadis, Ioannis; Papadaki, Maria; Giménez, Ignacio; Genovés, José Vicente Roig; Mylonas, Constantinos C Aquaculture, 594 , pp. 741401, 2025, ISSN: 00448486. @article{lancerotto_overcoming_2025, title = {Overcoming dysfunctional gametogenesis in hatchery-produced greater amberjack, Seriola dumerili using recombinant gonadotropins, and spawning induction using gonadotropin releasing hormone agonist-loaded implants}, author = {Stefano Lancerotto and Ioannis Fakriadis and Maria Papadaki and Ignacio Giménez and José Vicente Roig Genovés and Constantinos C Mylonas}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848624008627 https://imbbc.hcmr.gr/wp-content/uploads/2025/01/2025-Lancerotto-AQUA-2.pdf}, doi = {10.1016/j.aquaculture.2024.741401}, issn = {00448486}, year = {2025}, date = {2025-01-21}, urldate = {2025-01-21}, journal = {Aquaculture}, volume = {594}, pages = {741401}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Dimitriadis, C; Marampouti, C; Calò, A; Franco, Di A; Giakoumi, S; Franco, Di E; Lorenzo, Di M; Gerovasileiou, V; Guidetti, P; Pey, A; Sini, M; Sourbès, L Marine Environmental Research, 193 , pp. 106293, 2025, ISSN: 01411136. @article{dimitriadis_evaluating_2024, title = {Evaluating the long term effectiveness of a Mediterranean marine protected area to tackle the effects of invasive and range expanding herbivorous fish on rocky reefs}, author = {C Dimitriadis and C Marampouti and A Calò and A Di Franco and S Giakoumi and E Di Franco and M Di Lorenzo and V Gerovasileiou and P Guidetti and A Pey and M Sini and L Sourbès}, url = {https://linkinghub.elsevier.com/retrieve/pii/S014111362300421X /wp-content/uploads/2025/06/PREPRINT-Dimitriadis-et-al-2024.pdf}, doi = {10.1016/j.marenvres.2023.106293}, issn = {01411136}, year = {2025}, date = {2025-01-13}, urldate = {2025-06-12}, journal = {Marine Environmental Research}, volume = {193}, pages = {106293}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Kourkouta, Chara; Tsipourlianos, Andreas; Papandroulakis, Nikos; Moutou, Katerina A; Koumoundouros, George Ray‐Resorption Syndrome in European Seabass, textitDicentrarchus labrax (Linnaeus, 1758) Journal Article Journal of Fish Diseases, 48 (2), pp. e14042, 2025, ISSN: 0140-7775, 1365-2761. @article{kourkouta_rayresorption_2025, title = {Ray‐Resorption Syndrome in European Seabass, textitDicentrarchus labrax (Linnaeus, 1758)}, author = {Chara Kourkouta and Andreas Tsipourlianos and Nikos Papandroulakis and Katerina A Moutou and George Koumoundouros}, url = {https://onlinelibrary.wiley.com/doi/10.1111/jfd.14042 https://imbbc.hcmr.gr/wp-content/uploads/2025/01/2025-Kourkouta-JFD-4.pdf}, doi = {10.1111/jfd.14042}, issn = {0140-7775, 1365-2761}, year = {2025}, date = {2025-01-13}, urldate = {2025-01-13}, journal = {Journal of Fish Diseases}, volume = {48}, number = {2}, pages = {e14042}, abstract = {ABSTRACT Fin abnormalities are common in reared fish. They mainly consist of partial to complete lack of rays and severe abnormalities of fin‐supporting skeletal elements, which develop during the larval stage, up to the completion of fin skeleton ontogeny. This study reports a new abnormal condition, ray‐resorption syndrome (RSS), which developed after the completion of fin ontogeny in initially normal European seabass larvae, leading to extensive loss of fin rays. At 49 days post‐fertilisation (dpf) (15 mm standard length, SL) all fish presented normal fins. However, nineteen days later (21 mm SL), significant fin damage, characterised by ray loss or fractures, was observed in all studied populations. The dorsal fin was most affected (55%–84%), followed by the pelvic (27%–53%) and anal fins (7%–17%). Microscopically, multiple non‐mineralised areas resembling resorption lacunae were evident along all fin lepidotrichia. By 98 dpf (40 mm SL), the fin‐ray loss had advanced, reaching its higher frequency in the dorsal (74%–83%) and caudal fins (71%–94%). Gene expression analysis revealed a significant upregulation of bglap (osteoblast maturation marker), acp5a (osteoclast maturation marker) and mmp13a (extracellular‐matrix remodelling marker) in RSS specimens. The results are discussed in respect of the possible causative factors of RSS.}, keywords = {}, pubstate = {published}, tppubtype = {article} } ABSTRACT Fin abnormalities are common in reared fish. They mainly consist of partial to complete lack of rays and severe abnormalities of fin‐supporting skeletal elements, which develop during the larval stage, up to the completion of fin skeleton ontogeny. This study reports a new abnormal condition, ray‐resorption syndrome (RSS), which developed after the completion of fin ontogeny in initially normal European seabass larvae, leading to extensive loss of fin rays. At 49 days post‐fertilisation (dpf) (15 mm standard length, SL) all fish presented normal fins. However, nineteen days later (21 mm SL), significant fin damage, characterised by ray loss or fractures, was observed in all studied populations. The dorsal fin was most affected (55%–84%), followed by the pelvic (27%–53%) and anal fins (7%–17%). Microscopically, multiple non‐mineralised areas resembling resorption lacunae were evident along all fin lepidotrichia. By 98 dpf (40 mm SL), the fin‐ray loss had advanced, reaching its higher frequency in the dorsal (74%–83%) and caudal fins (71%–94%). Gene expression analysis revealed a significant upregulation of bglap (osteoblast maturation marker), acp5a (osteoclast maturation marker) and mmp13a (extracellular‐matrix remodelling marker) in RSS specimens. The results are discussed in respect of the possible causative factors of RSS. |
Bušelić, Ivana; Trumbić, Željka; Hrabar, Jerko; Lepen-Pleić, Ivana; Šegvić-Bubić, Tanja; Kaitetzidou, Elisavet; Tibaldi, Emilio; Bočina, Ivana; Grubišić, Leon; Sarropoulou, Elena 594 , pp. 741385, 2025, ISSN: 00448486. @article{buselic_unravelling_2025, title = {Unravelling the intricate language of fish guts: Impact of plant-based vs. plant-insect-poultry-based diets on intestinal pathways in European seabass}, author = {Ivana Bušelić and Željka Trumbić and Jerko Hrabar and Ivana Lepen-Pleić and Tanja Šegvić-Bubić and Elisavet Kaitetzidou and Emilio Tibaldi and Ivana Bočina and Leon Grubišić and Elena Sarropoulou}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848624008469 https://imbbc.hcmr.gr/wp-content/uploads/2025/01/2025-Buselic-AQUA-1.pdf}, doi = {10.1016/j.aquaculture.2024.741385}, issn = {00448486}, year = {2025}, date = {2025-01-08}, urldate = {2025-01-08}, volume = {594}, pages = {741385}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Pavloudi, Christina; Santi, Ioulia; Azua, Iñigo; Baña, Zuriñe; Bastianini, Mauro; Belser, Caroline; Bilbao, Jone; Bitz-Thorsen, Julie; Broudin, Caroline; Camusat, Mathieu; Cancio, Ibon; Caray-Counil, Louis; Casotti, Raffaella; Castel, Jade; Comtet, Thierry; Cox, Cymon; Daguin, Claire; Cerio, Oihane Díaz De; Exter, Katrina; Fauvelot, Cécile; Frada, Miguel; Galand, Pierre; Garczarek, Laurence; Fernández, Jose González; Guillou, Laure; Hablützel, Pascal; Heynderickx, Hanneloor; Houbin, Céline; Kervella, Anne; Krystallas, Apostolos; Lagaisse, Rune; Laroquette, Arnaud; Lescure, Lyvia; Lopes, Eva; Loulakaki, Melina; Louro, Bruno; Magalhaes, Catarina; Maidanou, Maria; Margiotta, Francesca; Montresor, Marina; Not, Fabrice; Paredes, Estefanía; Percopo, Isabella; Péru, Erwan; Poulain, Julie; Præbel, Kim; Rigaut-Jalabert, Fabienne; Romac, Sarah; Stavroulaki, Melanthia; Troncoso, Jesús Souza; Thiébaut, Eric; Thomas, Wilfried; Tkacz, Andrzej; Trano, Anna Chiara; Wincker, Patrick; Pade, Nicolas First release of the European marine omics biodiversity observation network (EMO BON) shotgun metagenomics data from water and sediment samples Journal Article Biodiversity Data Journal, 13 , pp. e143585, 2025, ISSN: 1314-2828, 1314-2836. @article{pavloudi_first_2025, title = {First release of the European marine omics biodiversity observation network (EMO BON) shotgun metagenomics data from water and sediment samples}, author = {Christina Pavloudi and Ioulia Santi and Iñigo Azua and Zuriñe Baña and Mauro Bastianini and Caroline Belser and Jone Bilbao and Julie Bitz-Thorsen and Caroline Broudin and Mathieu Camusat and Ibon Cancio and Louis Caray-Counil and Raffaella Casotti and Jade Castel and Thierry Comtet and Cymon Cox and Claire Daguin and Oihane Díaz De Cerio and Katrina Exter and Cécile Fauvelot and Miguel Frada and Pierre Galand and Laurence Garczarek and Jose González Fernández and Laure Guillou and Pascal Hablützel and Hanneloor Heynderickx and Céline Houbin and Anne Kervella and Apostolos Krystallas and Rune Lagaisse and Arnaud Laroquette and Lyvia Lescure and Eva Lopes and Melina Loulakaki and Bruno Louro and Catarina Magalhaes and Maria Maidanou and Francesca Margiotta and Marina Montresor and Fabrice Not and Estefanía Paredes and Isabella Percopo and Erwan Péru and Julie Poulain and Kim Præbel and Fabienne Rigaut-Jalabert and Sarah Romac and Melanthia Stavroulaki and Jesús Souza Troncoso and Eric Thiébaut and Wilfried Thomas and Andrzej Tkacz and Anna Chiara Trano and Patrick Wincker and Nicolas Pade}, url = {https://bdj.pensoft.net/article/143585/ /wp-content/uploads/2025/04/2025-Pavloudi-BDJ-17.pdf}, doi = {10.3897/BDJ.13.e143585}, issn = {1314-2828, 1314-2836}, year = {2025}, date = {2025-01-01}, urldate = {2025-04-03}, journal = {Biodiversity Data Journal}, volume = {13}, pages = {e143585}, abstract = {The European Marine Omics Biodiversity Observation Network (EMO BON) is an initiative of the European Marine Biological Resource Centre (EMBRC) to establish a persistent genomic observatory amongst designated European coastal marine sites, sharing the same protocols for sampling and data curation. Environmental samples are collected from the water column and, at some sites, soft sediments and hard substrates (Autonomous Reef Monitoring Structures - ARMS), together with a set of mandatory and discretionary metadata (including Essential Ocean Variables - EOVs). Samples are collected following standardised protocols at regular and specified intervals and sequenced in large six-monthly batches at a centralised sequencing facility. The use of standard operating procedures (SOPs) during data collection, library preparation and sequencing aims to provide uniformity amongst the data collected from the sites. Coupled with strict adherence to open and FAIR (Findable, Accessible, Interoperable, Reusable) data principles, this ensures maximum comparability amongst samples and enhances reusability and interoperability of the data with other data sources. The observatory network was launched in June 2021, when the first sampling campaign took place.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The European Marine Omics Biodiversity Observation Network (EMO BON) is an initiative of the European Marine Biological Resource Centre (EMBRC) to establish a persistent genomic observatory amongst designated European coastal marine sites, sharing the same protocols for sampling and data curation. Environmental samples are collected from the water column and, at some sites, soft sediments and hard substrates (Autonomous Reef Monitoring Structures - ARMS), together with a set of mandatory and discretionary metadata (including Essential Ocean Variables - EOVs). Samples are collected following standardised protocols at regular and specified intervals and sequenced in large six-monthly batches at a centralised sequencing facility. The use of standard operating procedures (SOPs) during data collection, library preparation and sequencing aims to provide uniformity amongst the data collected from the sites. Coupled with strict adherence to open and FAIR (Findable, Accessible, Interoperable, Reusable) data principles, this ensures maximum comparability amongst samples and enhances reusability and interoperability of the data with other data sources. The observatory network was launched in June 2021, when the first sampling campaign took place. |
2024 |
Voskakis, Dimitris; Kelasidi, Eleni; Papandroulakis, Nikos Design and Development of Underwater Robotic Arm for Automated Camera Calibration for Aquatic Environment Inproceedings 2024 10th International Conference on Automation, Robotics and Applications (ICARA), pp. 50–56, IEEE, Athens, Greece, 2024, ISBN: 9798350394245. @inproceedings{voskakis_design_2024, title = {Design and Development of Underwater Robotic Arm for Automated Camera Calibration for Aquatic Environment}, author = {Dimitris Voskakis and Eleni Kelasidi and Nikos Papandroulakis}, url = {https://ieeexplore.ieee.org/document/10552983/ https://imbbc.hcmr.gr/wp-content/uploads/2025/01/ICARA-2024-Voskakis-pre-print.pdf}, doi = {10.1109/ICARA60736.2024.10552983}, isbn = {9798350394245}, year = {2024}, date = {2024-12-31}, urldate = {2025-01-10}, booktitle = {2024 10th International Conference on Automation, Robotics and Applications (ICARA)}, pages = {50--56}, publisher = {IEEE}, address = {Athens, Greece}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Mladineo, Ivona; Hrabar, Jerko; Trumbić, Željka; Rasouli-Dogaheh, Somayeh; Beraldo, Paola; Rigos, George; Palenzuela, Oswado; Sitjà-Bobadilla, Ariadna Aquaculture, 598 , pp. 741997, 2024, ISSN: 00448486. @article{mladineo_mediterranean-wide_2025, title = {Mediterranean-wide transfer of the polyopisthocotylean Sparicotyle chrysophrii between wild sparids and farmed gilthead seabream (Sparus aurata) inferred by ddRAD loci}, author = {Ivona Mladineo and Jerko Hrabar and Željka Trumbić and Somayeh Rasouli-Dogaheh and Paola Beraldo and George Rigos and Oswado Palenzuela and Ariadna Sitjà-Bobadilla}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848624014595 https://imbbc.hcmr.gr/wp-content/uploads/2025/01/2024-Mladineo...Rigos-et-al-pre-print-48.pdf}, doi = {10.1016/j.aquaculture.2024.741997}, issn = {00448486}, year = {2024}, date = {2024-12-30}, urldate = {2025-01-08}, journal = {Aquaculture}, volume = {598}, pages = {741997}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Livanou, Eleni; Sauzède, Raphaëlle; Psarra, Stella; Mandalakis, Manolis; Dall’Olmo, Giorgio; Brewin, Robert J W; Raitsos, Dionysios E Evaluating MULTIOBS Chlorophyll-a with Ground-Truth Observations in the Eastern Mediterranean Sea Journal Article Remote Sensing, 16 (24), pp. 4705, 2024, ISSN: 2072-4292. @article{livanou_evaluating_2024, title = {Evaluating MULTIOBS Chlorophyll-a with Ground-Truth Observations in the Eastern Mediterranean Sea}, author = {Eleni Livanou and Raphaëlle Sauzède and Stella Psarra and Manolis Mandalakis and Giorgio Dall’Olmo and Robert J W Brewin and Dionysios E Raitsos}, url = {https://www.mdpi.com/2072-4292/16/24/4705 /wp-content/uploads/2024/12/2024-Livanou-Remote-Sensing-49.pdf}, doi = {10.3390/rs16244705}, issn = {2072-4292}, year = {2024}, date = {2024-12-23}, urldate = {2024-12-23}, journal = {Remote Sensing}, volume = {16}, number = {24}, pages = {4705}, abstract = {Satellite-derived observations of ocean colour provide continuous data on chlorophyll-a concentration (Chl-a) at global scales but are limited to the ocean’s surface. So far, biogeochemical models have been the only means of generating continuous vertically resolved Chl-a profiles on a regular grid. MULTIOBS is a multi-observations oceanographic dataset that provides depth-resolved biological data based on merged satellite- and Argo-derived in situ hydrological data. This product is distributed by the European Union’s Copernicus Marine Service and offers global multiyear, gridded Chl-a profiles within the ocean’s productive zone at a weekly temporal resolution. MULTIOBS addresses the scarcity of observation-based vertically resolved Chl-a datasets, particularly in less sampled regions like the Eastern Mediterranean Sea (EMS). Here, we conduct an independent evaluation of the MULTIOBS dataset in the oligotrophic waters of the EMS using in situ Chl-a profiles. Our analysis shows that this product accurately and precisely retrieves Chl-a across depths, with a slight 1% overestimation and an observed 1.5-fold average deviation between in situ data and MULTIOBS estimates. The deep chlorophyll maximum (DCM) is adequately estimated by MULTIOBS both in terms of positioning (root mean square error}, keywords = {}, pubstate = {published}, tppubtype = {article} } Satellite-derived observations of ocean colour provide continuous data on chlorophyll-a concentration (Chl-a) at global scales but are limited to the ocean’s surface. So far, biogeochemical models have been the only means of generating continuous vertically resolved Chl-a profiles on a regular grid. MULTIOBS is a multi-observations oceanographic dataset that provides depth-resolved biological data based on merged satellite- and Argo-derived in situ hydrological data. This product is distributed by the European Union’s Copernicus Marine Service and offers global multiyear, gridded Chl-a profiles within the ocean’s productive zone at a weekly temporal resolution. MULTIOBS addresses the scarcity of observation-based vertically resolved Chl-a datasets, particularly in less sampled regions like the Eastern Mediterranean Sea (EMS). Here, we conduct an independent evaluation of the MULTIOBS dataset in the oligotrophic waters of the EMS using in situ Chl-a profiles. Our analysis shows that this product accurately and precisely retrieves Chl-a across depths, with a slight 1% overestimation and an observed 1.5-fold average deviation between in situ data and MULTIOBS estimates. The deep chlorophyll maximum (DCM) is adequately estimated by MULTIOBS both in terms of positioning (root mean square error |
Apostolaki, Eugenia T; Lavery, Paul S; Litsi‐Mizan, Victoria; Serrano, Eduard; Inostroza, Karina; Gerakaris, Vasilis; Dailianis, Thanos; Glampedakis, Julius; Holitzki, Tara; Johnson, Erik; Mateo, Miguel A; Serrano, Oscar Patterns of Carbon and Nitrogen Accumulation in Seagrass ( textitPosidonia oceanica ) Meadows of the Eastern Mediterranean Sea Journal Article Journal of Geophysical Research: Biogeosciences, 129 (12), pp. e2024JG008163, 2024, ISSN: 2169-8953, 2169-8961. @article{apostolaki_patterns_2024, title = {Patterns of Carbon and Nitrogen Accumulation in Seagrass ( textitPosidonia oceanica ) Meadows of the Eastern Mediterranean Sea}, author = {Eugenia T Apostolaki and Paul S Lavery and Victoria Litsi‐Mizan and Eduard Serrano and Karina Inostroza and Vasilis Gerakaris and Thanos Dailianis and Julius Glampedakis and Tara Holitzki and Erik Johnson and Miguel A Mateo and Oscar Serrano}, url = {https://agupubs.onlinelibrary.wiley.com/doi/10.1029/2024JG008163 https://imbbc.hcmr.gr/wp-content/uploads/2024/12/2024-Apostolaki-JGR-BiosSci-47-1.pdf}, doi = {10.1029/2024JG008163}, issn = {2169-8953, 2169-8961}, year = {2024}, date = {2024-12-18}, urldate = {2024-12-18}, journal = {Journal of Geophysical Research: Biogeosciences}, volume = {129}, number = {12}, pages = {e2024JG008163}, abstract = {Abstract The variability in stocks and accumulation rates of organic carbon (C org ), nitrogen (N), and carbonate (CaCO 3 ) was studied in fifteen Posidonia oceanica meadows spread throughout the South Aegean Sea (Greece). In addition, the abiotic and biotic drivers determining the pattern of variability in the accumulation rates were assessed by exploring the influence of sediment characteristics, seagrass traits, and environmental settings. The meadows supported on average (±STDEV) 14.6 ± 5.0 kg C org m −2 , 0.47 ± 0.17 kg N m −2 , and 249 ± 210 kg CaCO 3 m −2 in the top meter of their sediments, with mean accumulation rates over the last 500 years of 33.6 ± 23.6 g C org m −2 yr −1 , 1.00 ± 0.62 g N m −2 yr −1 , and 405 ± 336 g CaCO 3 m −2 yr −1 across sites. A redundancy analysis (RDA) explained 70% of the variation in C org , N, and CaCO 3 accumulation rates, with three sediment characteristics (i.e., sediment C org :N and C org :C inorg ratios and P . oceanica contribution to the sediment C org pool) emerging as the primary set of factors shaping the accumulation of matter, followed by seagrass traits (i.e., leaf biomass and rhizome elongation) and environmental variables (i.e., suspended organic matter). The high degree of variability within the region emphasizes the need for fine‐scale assessments to understand the local conditions influencing sequestration. Our findings underscored the critical role of seagrass meadows in carbon and nitrogen sequestration in the region, urging conservation efforts to protect these ecosystems and prevent potential losses of stored carbon and nitrogen following seagrass degradation. , Plain Language Summary In the present study, we explored the abiotic and biotic factors influencing the accumulation patterns of carbon, nitrogen, and carbonate in 15 seagrass ( Posidonia oceanica ) meadows across the South Aegean Sea (Greece). Sediment characteristics (i.e., sediment C org :N and C org :C inorg ratios and P . ocenica contribution to the sediment C org pool) mainly drove the pattern of carbon, nitrogen, and carbonate accumulation, with seagrass traits (i.e., leaf biomass and rhizome elongation) and environmental conditions (i.e., suspended organic matter) contributing to a lesser extent. Overall, seagrass meadows play a crucial role in storing carbon and nitrogen in the studied region, serving as important natural sinks, and thus, protecting these ecosystems is important to prevent the loss of these stored elements. Additionally, understanding the factors that shape the variability in Posidonia oceanica storage capacity is crucial for management and conservation efforts. , Key Points Seagrass ( Posidonia oceanica ) meadows of the South Aegean Sea (Greece) represent important natural sinks for organic carbon, nitrogen, and carbonate. The accumulation pattern of organic carbon, nitrogen, and carbonate in the underlying seagrass sediments was mainly driven by sediment characteristics, followed by seagrass traits and environmental conditions. Fine‐scale assessments are crucial for understanding the local factors shaping the storage capacity of seagrass meadows to support management decisions.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract The variability in stocks and accumulation rates of organic carbon (C org ), nitrogen (N), and carbonate (CaCO 3 ) was studied in fifteen Posidonia oceanica meadows spread throughout the South Aegean Sea (Greece). In addition, the abiotic and biotic drivers determining the pattern of variability in the accumulation rates were assessed by exploring the influence of sediment characteristics, seagrass traits, and environmental settings. The meadows supported on average (±STDEV) 14.6 ± 5.0 kg C org m −2 , 0.47 ± 0.17 kg N m −2 , and 249 ± 210 kg CaCO 3 m −2 in the top meter of their sediments, with mean accumulation rates over the last 500 years of 33.6 ± 23.6 g C org m −2 yr −1 , 1.00 ± 0.62 g N m −2 yr −1 , and 405 ± 336 g CaCO 3 m −2 yr −1 across sites. A redundancy analysis (RDA) explained 70% of the variation in C org , N, and CaCO 3 accumulation rates, with three sediment characteristics (i.e., sediment C org :N and C org :C inorg ratios and P . oceanica contribution to the sediment C org pool) emerging as the primary set of factors shaping the accumulation of matter, followed by seagrass traits (i.e., leaf biomass and rhizome elongation) and environmental variables (i.e., suspended organic matter). The high degree of variability within the region emphasizes the need for fine‐scale assessments to understand the local conditions influencing sequestration. Our findings underscored the critical role of seagrass meadows in carbon and nitrogen sequestration in the region, urging conservation efforts to protect these ecosystems and prevent potential losses of stored carbon and nitrogen following seagrass degradation. , Plain Language Summary In the present study, we explored the abiotic and biotic factors influencing the accumulation patterns of carbon, nitrogen, and carbonate in 15 seagrass ( Posidonia oceanica ) meadows across the South Aegean Sea (Greece). Sediment characteristics (i.e., sediment C org :N and C org :C inorg ratios and P . ocenica contribution to the sediment C org pool) mainly drove the pattern of carbon, nitrogen, and carbonate accumulation, with seagrass traits (i.e., leaf biomass and rhizome elongation) and environmental conditions (i.e., suspended organic matter) contributing to a lesser extent. Overall, seagrass meadows play a crucial role in storing carbon and nitrogen in the studied region, serving as important natural sinks, and thus, protecting these ecosystems is important to prevent the loss of these stored elements. Additionally, understanding the factors that shape the variability in Posidonia oceanica storage capacity is crucial for management and conservation efforts. , Key Points Seagrass ( Posidonia oceanica ) meadows of the South Aegean Sea (Greece) represent important natural sinks for organic carbon, nitrogen, and carbonate. The accumulation pattern of organic carbon, nitrogen, and carbonate in the underlying seagrass sediments was mainly driven by sediment characteristics, followed by seagrass traits and environmental conditions. Fine‐scale assessments are crucial for understanding the local factors shaping the storage capacity of seagrass meadows to support management decisions. |
Rotter, Ana; Varamogianni-Mamatsi, Despoina; Pobirk, Alenka Zvonar; Matjaž, Mirjam Gosenca; Cueto, Mercedes; Díaz-Marrero, Ana R; Jónsdóttir, Rósa; Sveinsdóttir, Kolbrún; Catalá, Teresa S; Romano, Giovanna; Guler, Bahar Aslanbay; Atak, Eylem; Zrimec, Maja Berden; Bosch, Daniel; Deniz, Irem; Gaudêncio, Susana P; Grigalionyte-Bembič, Ernesta; Klun, Katja; Zidar, Luen; Rius, Anna Coll; Baebler, Špela; Bilela, Lada Lukić; Rinkevich, Baruch; Mandalakis, Manolis Marine cosmetics and the blue bioeconomy: From sourcing to success stories Journal Article iScience, 27 (12), pp. 111339, 2024, ISSN: 25890042. @article{rotter_marine_2024, title = {Marine cosmetics and the blue bioeconomy: From sourcing to success stories}, author = {Ana Rotter and Despoina Varamogianni-Mamatsi and Alenka Zvonar Pobirk and Mirjam Gosenca Matjaž and Mercedes Cueto and Ana R Díaz-Marrero and Rósa Jónsdóttir and Kolbrún Sveinsdóttir and Teresa S Catalá and Giovanna Romano and Bahar Aslanbay Guler and Eylem Atak and Maja Berden Zrimec and Daniel Bosch and Irem Deniz and Susana P Gaudêncio and Ernesta Grigalionyte-Bembič and Katja Klun and Luen Zidar and Anna Coll Rius and Špela Baebler and Lada Lukić Bilela and Baruch Rinkevich and Manolis Mandalakis}, url = {https://linkinghub.elsevier.com/retrieve/pii/S2589004224025641 /wp-content/uploads/2024/12/2024-Rotter-iScience-46.pdf}, doi = {10.1016/j.isci.2024.111339}, issn = {25890042}, year = {2024}, date = {2024-12-09}, urldate = {2024-12-10}, journal = {iScience}, volume = {27}, number = {12}, pages = {111339}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Skliros, Dimitrios; Kostakou, Maria; Kokkari, Constantina; Tsertou, Maria Ioanna; Pavloudi, Christina; Zafeiropoulos, Haris; Katharios, Pantelis; Flemetakis, Emmanouil Microorganisms, 12 (11), pp. 2281, 2024, ISSN: 2076-2607. @article{skliros_unveiling_2024, title = {Unveiling Emerging Opportunistic Fish Pathogens in Aquaculture: A Comprehensive Seasonal Study of Microbial Composition in Mediterranean Fish Hatcheries}, author = {Dimitrios Skliros and Maria Kostakou and Constantina Kokkari and Maria Ioanna Tsertou and Christina Pavloudi and Haris Zafeiropoulos and Pantelis Katharios and Emmanouil Flemetakis}, url = {https://www.mdpi.com/2076-2607/12/11/2281 https://imbbc.hcmr.gr/wp-content/uploads/2024/11/2024-Skliros-microorganisms-45.pdf}, doi = {10.3390/microorganisms12112281}, issn = {2076-2607}, year = {2024}, date = {2024-11-12}, urldate = {2024-11-12}, journal = {Microorganisms}, volume = {12}, number = {11}, pages = {2281}, abstract = {The importance of microbial communities in fish hatcheries for fish health and welfare has been recognized, with several studies mapping these communities during healthy rearing conditions and disease outbreaks. In this study, we analyzed the bacteriome of the live feeds, such as microalgae, rotifers, and Artemia, used in fish hatcheries that produce Mediterranean species. Our goal was to provide baseline information about their structure, emphasizing in environmental putative fish pathogenic bacteria. We conducted 16S rRNA amplicon Novaseq sequencing for our analysis, and we inferred 46,745 taxonomically annotated ASVs. Results showed that incoming environmental water plays a significant role in the presence of important taxa that constitute presumptive pathogens. Bio-statistical analyses revealed a relatively stable bacteriome among seasonal samplings for every hatchery but a diverse bacteriome between sampling stations and a distinct core bacteriome for each hatchery. Analysis of putative opportunistic fish pathogenic genera revealed some co-occurrence correlation events and a high average relative abundance of Vibrio, Tenacibaculum, and Photobacterium genera in live feeds, reaching a grand mean average of up to 7.3% for the hatchery of the Hellenic Center of Marine Research (HCMR), 12% for Hatchery A, and 11.5% for Hatchery B. Mapping the bacteriome in live feeds is pivotal for understanding the marine environment and distinct aquaculture practices and can guide improvements in hatchery management, enhancing fish health and sustainability in the Mediterranean region.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The importance of microbial communities in fish hatcheries for fish health and welfare has been recognized, with several studies mapping these communities during healthy rearing conditions and disease outbreaks. In this study, we analyzed the bacteriome of the live feeds, such as microalgae, rotifers, and Artemia, used in fish hatcheries that produce Mediterranean species. Our goal was to provide baseline information about their structure, emphasizing in environmental putative fish pathogenic bacteria. We conducted 16S rRNA amplicon Novaseq sequencing for our analysis, and we inferred 46,745 taxonomically annotated ASVs. Results showed that incoming environmental water plays a significant role in the presence of important taxa that constitute presumptive pathogens. Bio-statistical analyses revealed a relatively stable bacteriome among seasonal samplings for every hatchery but a diverse bacteriome between sampling stations and a distinct core bacteriome for each hatchery. Analysis of putative opportunistic fish pathogenic genera revealed some co-occurrence correlation events and a high average relative abundance of Vibrio, Tenacibaculum, and Photobacterium genera in live feeds, reaching a grand mean average of up to 7.3% for the hatchery of the Hellenic Center of Marine Research (HCMR), 12% for Hatchery A, and 11.5% for Hatchery B. Mapping the bacteriome in live feeds is pivotal for understanding the marine environment and distinct aquaculture practices and can guide improvements in hatchery management, enhancing fish health and sustainability in the Mediterranean region. |
Lanza-Arroyo, Pablo; Sempere-Valverde, Juan; Digenis, Markos; Remón, José Miguel; Moreno, Diego; Barrajón, Agustín; Linde, Antonio De La; Arroyo, Maria Del Carmen; Fernández-Casado, Manuel; Mallofret, Eugenio; Sánchez-Tocino, Luis; Gerovasileiou, Vasilis; Navarro-Barranco, Carlos Baseline for marine cave monitoring strategies in the Alboran Sea using modified Cave Ecosystem-Based Quality Index (CavEBQI) Journal Article Marine Pollution Bulletin, 209 , pp. 117065, 2024, ISSN: 0025326X. @article{lanza-arroyo_baseline_2024, title = {Baseline for marine cave monitoring strategies in the Alboran Sea using modified Cave Ecosystem-Based Quality Index (CavEBQI)}, author = {Pablo Lanza-Arroyo and Juan Sempere-Valverde and Markos Digenis and José Miguel Remón and Diego Moreno and Agustín Barrajón and Antonio De La Linde and Maria Del Carmen Arroyo and Manuel Fernández-Casado and Eugenio Mallofret and Luis Sánchez-Tocino and Vasilis Gerovasileiou and Carlos Navarro-Barranco}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0025326X24010427 /wp-content/uploads/2025/07/2024-Lanza-MPB-52.pdf}, doi = {10.1016/j.marpolbul.2024.117065}, issn = {0025326X}, year = {2024}, date = {2024-10-11}, urldate = {2025-07-08}, journal = {Marine Pollution Bulletin}, volume = {209}, pages = {117065}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Daniso, Enrico; Sarropoulou, Elena; Kaitetzidou, Elisavet; Beraldo, Paola; Tibaldi, Emilio; Cerri, Roberto; Cardinaletti, Gloriana Effect of increasing levels of Hermetia illucens in a fishmeal-free diet at sea bream (Sparus aurata, L.) gastrointestinal level Journal Article Aquaculture Reports, 39 , pp. 102410, 2024, ISSN: 23525134. @article{daniso_effect_2024, title = {Effect of increasing levels of Hermetia illucens in a fishmeal-free diet at sea bream (Sparus aurata, L.) gastrointestinal level}, author = {Enrico Daniso and Elena Sarropoulou and Elisavet Kaitetzidou and Paola Beraldo and Emilio Tibaldi and Roberto Cerri and Gloriana Cardinaletti}, url = {https://linkinghub.elsevier.com/retrieve/pii/S2352513424004988 /wp-content/uploads/2024/10/2024-Daniso-AquaReports-44.pdf}, doi = {10.1016/j.aqrep.2024.102410}, issn = {23525134}, year = {2024}, date = {2024-10-09}, urldate = {2024-10-09}, journal = {Aquaculture Reports}, volume = {39}, pages = {102410}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Kotsiri, Mado; Kleidas, Ioannis; Kogiannou, Dimitra; Vryonides, Katerina; Papadouli, Christina; Golomazou, Eleni; Rigos, George Evaluation of the long-term effects of formaldehyde on the physiology of the Mediterranean mussel, Mytilus galloprovincialis Journal Article Chemosphere, 364 , pp. 143190, 2024, ISSN: 00456535. @article{kotsiri_evaluation_2024, title = {Evaluation of the long-term effects of formaldehyde on the physiology of the Mediterranean mussel, Mytilus galloprovincialis}, author = {Mado Kotsiri and Ioannis Kleidas and Dimitra Kogiannou and Katerina Vryonides and Christina Papadouli and Eleni Golomazou and George Rigos}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0045653524020873 https://imbbc.hcmr.gr/wp-content/uploads/2024/10/2024-Kotsiri-43-pre-print.pdf}, doi = {10.1016/j.chemosphere.2024.143190}, issn = {00456535}, year = {2024}, date = {2024-09-30}, urldate = {2024-10-01}, journal = {Chemosphere}, volume = {364}, pages = {143190}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Reverté, Jaume; Rambla-Alegre, Maria; Sanchez-Henao, Andres; Mandalakis, Manolis; Peristeraki, Panagiota; Molgó, Jordi; Diogène, Jorge; Sureda, Francesc X; Campàs, Mònica Toxicity Equivalency Factors for Tetrodotoxin Analogues Determined with Automated Patch Clamp on Voltage-Gated Sodium Channels in Neuro-2a Cells Journal Article Journal of Agricultural and Food Chemistry, 72 (32), pp. 18192–18200, 2024, ISSN: 0021-8561, 1520-5118. @article{reverte_toxicity_2024, title = {Toxicity Equivalency Factors for Tetrodotoxin Analogues Determined with Automated Patch Clamp on Voltage-Gated Sodium Channels in Neuro-2a Cells}, author = {Jaume Reverté and Maria Rambla-Alegre and Andres Sanchez-Henao and Manolis Mandalakis and Panagiota Peristeraki and Jordi Molgó and Jorge Diogène and Francesc X Sureda and Mònica Campàs}, url = {https://pubs.acs.org/doi/10.1021/acs.jafc.4c04321 https://imbbc.hcmr.gr/wp-content/uploads/2024/09/2024-Reverte-JAFC-42-pre-print.pdf}, doi = {10.1021/acs.jafc.4c04321}, issn = {0021-8561, 1520-5118}, year = {2024}, date = {2024-09-26}, urldate = {2024-09-26}, journal = {Journal of Agricultural and Food Chemistry}, volume = {72}, number = {32}, pages = {18192--18200}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Skouradakis, Grigorios; Vernadou, Emmanouela; Koulouri, Panayota; Dailianis, Thanos Mass mortality of the invasive echinoid Diadema setosum (Leske, 1778) in Crete, East Mediterranean Sea Journal Article Mediterranean Marine Science, 25 (2), pp. 480–483, 2024, ISSN: 1791-6763, 1108-393X. @article{skouradakis_mass_2024, title = {Mass mortality of the invasive echinoid Diadema setosum (Leske, 1778) in Crete, East Mediterranean Sea}, author = {Grigorios Skouradakis and Emmanouela Vernadou and Panayota Koulouri and Thanos Dailianis}, url = {https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/36447 https://imbbc.hcmr.gr/wp-content/uploads/2024/09/2024-Skouradakis-MMS-41.pdf}, doi = {10.12681/mms.36447}, issn = {1791-6763, 1108-393X}, year = {2024}, date = {2024-09-23}, urldate = {2024-09-23}, journal = {Mediterranean Marine Science}, volume = {25}, number = {2}, pages = {480--483}, abstract = {Diadema setosum is an echinoid of Indo-Pacific origin that invaded the Mediterranean Sea in 2006. It is an ecosystem engineer with an important ecological function in its native range, but it can have a detrimental effect on Mediterranean reefs. Recently in 2022 a mass mortality event (MME) affecting this species was recorded in the east Aegean Sea in the eastern part of the Mediterranean basin. We are reporting herein a westward progression of the MME in 2023 affecting established populations in various locations around the island of Crete.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Diadema setosum is an echinoid of Indo-Pacific origin that invaded the Mediterranean Sea in 2006. It is an ecosystem engineer with an important ecological function in its native range, but it can have a detrimental effect on Mediterranean reefs. Recently in 2022 a mass mortality event (MME) affecting this species was recorded in the east Aegean Sea in the eastern part of the Mediterranean basin. We are reporting herein a westward progression of the MME in 2023 affecting established populations in various locations around the island of Crete. |
Lavecchia, Anna; Virgilio, Caterina De; Mansi, Luigi; Manzari, Caterina; Mylonas, Constantinos C; Picardi, Ernesto; Pousis, Chrysovalentinos; Cox, Sharon N; Ventriglia, Gianluca; Zupa, Rosa; Pesole, Graziano; Corriero, Aldo Comparison of ovarian mRNA expression levels in wild and hatchery-produced greater amberjack Seriola dumerili Journal Article Scientific Reports, 14 (1), pp. 18034, 2024, ISSN: 2045-2322. @article{lavecchia_comparison_2024, title = {Comparison of ovarian mRNA expression levels in wild and hatchery-produced greater amberjack Seriola dumerili}, author = {Anna Lavecchia and Caterina De Virgilio and Luigi Mansi and Caterina Manzari and Constantinos C Mylonas and Ernesto Picardi and Chrysovalentinos Pousis and Sharon N Cox and Gianluca Ventriglia and Rosa Zupa and Graziano Pesole and Aldo Corriero}, url = {https://www.nature.com/articles/s41598-024-69091-2 https://imbbc.hcmr.gr/wp-content/uploads/2024/09/2024-Lavecchia-SciRep-40.pdf}, doi = {10.1038/s41598-024-69091-2}, issn = {2045-2322}, year = {2024}, date = {2024-09-16}, urldate = {2024-09-16}, journal = {Scientific Reports}, volume = {14}, number = {1}, pages = {18034}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Publications
2025 |
Seasonal Variation in Nutritional Value and Technical Quality of Lionfish (Pterois miles) from the Ionian and Aegean Seas Journal Article Foods, 14 (13), pp. 2353, 2025, ISSN: 2304-8158. |
Low Heat Flow in the Anhydros Basin, Aegean Sea, Recorded by Deep Subsurface Temperatures Journal Article Geophysical Research Letters, 52 (13), pp. e2025GL115919, 2025, ISSN: 0094-8276, 1944-8007. |
Investigating the Potential of Newly Isolated Microalgae Strains from the Ionian Sea (Greece) Cultured in an Open Raceway Pond Journal Article Applied Sciences, 15 (12), pp. 6680, 2025, ISSN: 2076-3417. |
Aquaculture, 609 , pp. 742820, 2025, ISSN: 0044-8486. |
Genome sequences of textitVibrio harveyi VH2 and textitVibrio harveyi Vhp1-sp Journal Article Microbiology Resource Announcements, pp. e00087–25, 2025, ISSN: 2576-098X. |
Aquaculture, 609 , pp. 742795, 2025, ISSN: 00448486. |
Disrupted darkness: the impact of anthropogenic light at night on melatonin secretion of Hermodice carunculata (Polychaeta, Annelida) Journal Article Photochemical & Photobiological Sciences, 24 (6), pp. 1031–1040, 2025, ISSN: 1474-905X, 1474-9092. |
Validation of QTL associated with resistance to Lernanthropus kroyeri in European seabass Journal Article Aquaculture, 608 , pp. 742763, 2025, ISSN: 00448486. |
Mediterranean Marine Science, 26 (2), pp. 327–340, 2025, ISSN: 1791-6763, 1108-393X. |
Marine Pollution Bulletin, 217 , pp. 118109, 2025, ISSN: 0025326X. |
Aquaculture Nutrition, 2025 (1), pp. 8885509, 2025, ISSN: 1353-5773, 1365-2095. |
Common carp (Cyprinus carpio) blood cells are modulated by a parasitic antimicrobial peptide, A-2S and Edwardsiella spp. strains Journal Article Fish & Shellfish Immunology, 164 , pp. 110427, 2025, ISSN: 10504648. |
Ultrastructural Insights Into a textitCandidatus Parilichlamydia sp. Infection of Gill Goblet Cells in Greater Amberjack Journal Article Journal of Fish Diseases, pp. e14146, 2025, ISSN: 0140-7775, 1365-2761. |
Multi-omics insights into the response of Aspergillus parasiticus to long-chain alkanes in relation to polyethylene modification Journal Article Environmental Pollution, 376 , pp. 126386, 2025, ISSN: 02697491. |
Aptamer-antibody sandwich lateral flow test for rapid visual detection of tetrodotoxin in pufferfish Journal Article Science of The Total Environment, 978 , pp. 179419, 2025, ISSN: 00489697. |
Spermatogenesis advancement in pre-pubertal meagre Argyrosomus regius treated with recombinant gonadotropins Journal Article Scientific Reports, 15 (1), pp. 15113, 2025, ISSN: 2045-2322. |
Marine Biotechnology, 27 (2), pp. 74, 2025, ISSN: 1436-2228, 1436-2236. |
Development of convergent adaptations reveal highly conserved early ontogenetic skull shape in fishes with amphibious vision Journal Article Scientific Reports, 15 (1), pp. 8411, 2025, ISSN: 2045-2322. |
Flying Robots Teach Floating Robots—A Machine Learning Approach for Marine Habitat Mapping Based on Combined Datasets Journal Article Journal of Marine Science and Engineering, 13 (3), pp. 611, 2025, ISSN: 2077-1312. |
Integrated functional genomic analysis identifies regulatory variants underlying a major QTL for disease resistance in European sea bass Journal Article BMC Biology, 23 (1), pp. 75, 2025, ISSN: 1741-7007. |
Aquaculture Reports, 42 , pp. 102739, 2025, ISSN: 23525134. |
Comparative phylogeography of four lizard taxa within an oceanic island Journal Article Molecular Phylogenetics and Evolution, 205 , pp. 108295, 2025, ISSN: 10557903. |
Phage Therapy in Aquaculture Incollection Elumalai, Preetham; Lakshmi, Sreeja (Ed.): Antimicrobial Resistance in Aquaculture and Aquatic Environments, pp. 229–255, Springer Nature Singapore, Singapore, 2025, ISBN: 9789819773190 9789819773206. |
Pathogens, 14 (3), pp. 247, 2025, ISSN: 2076-0817. |
High-quality genome assembly and annotation of Thalassiosira rotula (synonym of Thalassiosira gravida) Journal Article Scientific Data, 12 (1), pp. 310, 2025, ISSN: 2052-4463. |
Circulating miRNAs involved in the immune response of the European seabass (Dicentrarchus labrax) Journal Article Fish & Shellfish Immunology, 160 , pp. 110232, 2025, ISSN: 10504648. |
Protozoan Co‐Infection Drives Fish Mortality Event in Crete's Karteros River Journal Article Journal of Fish Diseases, pp. e14099, 2025, ISSN: 0140-7775, 1365-2761. |
DIGIT-KEY: an aid towards uniform 2D+ and 3D digitisation techniques within natural history collections Journal Article European Journal of Taxonomy, 976 , 2025, ISSN: 2118-9773. |
Freshness and Spoilage Patterns of Wild and Farmed Tropical Fish Species with Major Commercial Importance Originating from Saudi Arabian Waters Journal Article Foods, 14 (4), pp. 690, 2025, ISSN: 2304-8158. |
Vulnerability of benthic trait diversity across the Mediterranean Sea following mass mortality events Journal Article Nature Communications, 16 (1), pp. 1571, 2025, ISSN: 2041-1723. |
A Long-Term Ecological Research Data Set From the Marine Genetic Monitoring Program ARMS-MBON 2018–2020 Journal Article Molecular Ecology Resources, pp. e14073, 2025, ISSN: 1755-098X, 1755-0998. |
Acoustic tags versus camera—a case study on feeding behaviour of European seabass in sea cages Journal Article Frontiers in Marine Science, 11 , pp. 1497336, 2025, ISSN: 2296-7745. |
Genomic evaluation for body weight, length and growth estimates in meagre Argyrosomus regius Journal Article Aquaculture, 595 , pp. 741622, 2025, ISSN: 00448486. |
Aquaculture, 594 , pp. 741401, 2025, ISSN: 00448486. |
Marine Environmental Research, 193 , pp. 106293, 2025, ISSN: 01411136. |
Ray‐Resorption Syndrome in European Seabass, textitDicentrarchus labrax (Linnaeus, 1758) Journal Article Journal of Fish Diseases, 48 (2), pp. e14042, 2025, ISSN: 0140-7775, 1365-2761. |
594 , pp. 741385, 2025, ISSN: 00448486. |
First release of the European marine omics biodiversity observation network (EMO BON) shotgun metagenomics data from water and sediment samples Journal Article Biodiversity Data Journal, 13 , pp. e143585, 2025, ISSN: 1314-2828, 1314-2836. |
2024 |
Design and Development of Underwater Robotic Arm for Automated Camera Calibration for Aquatic Environment Inproceedings 2024 10th International Conference on Automation, Robotics and Applications (ICARA), pp. 50–56, IEEE, Athens, Greece, 2024, ISBN: 9798350394245. |
Aquaculture, 598 , pp. 741997, 2024, ISSN: 00448486. |
Evaluating MULTIOBS Chlorophyll-a with Ground-Truth Observations in the Eastern Mediterranean Sea Journal Article Remote Sensing, 16 (24), pp. 4705, 2024, ISSN: 2072-4292. |
Patterns of Carbon and Nitrogen Accumulation in Seagrass ( textitPosidonia oceanica ) Meadows of the Eastern Mediterranean Sea Journal Article Journal of Geophysical Research: Biogeosciences, 129 (12), pp. e2024JG008163, 2024, ISSN: 2169-8953, 2169-8961. |
Marine cosmetics and the blue bioeconomy: From sourcing to success stories Journal Article iScience, 27 (12), pp. 111339, 2024, ISSN: 25890042. |
Microorganisms, 12 (11), pp. 2281, 2024, ISSN: 2076-2607. |
Baseline for marine cave monitoring strategies in the Alboran Sea using modified Cave Ecosystem-Based Quality Index (CavEBQI) Journal Article Marine Pollution Bulletin, 209 , pp. 117065, 2024, ISSN: 0025326X. |
Effect of increasing levels of Hermetia illucens in a fishmeal-free diet at sea bream (Sparus aurata, L.) gastrointestinal level Journal Article Aquaculture Reports, 39 , pp. 102410, 2024, ISSN: 23525134. |
Evaluation of the long-term effects of formaldehyde on the physiology of the Mediterranean mussel, Mytilus galloprovincialis Journal Article Chemosphere, 364 , pp. 143190, 2024, ISSN: 00456535. |
Toxicity Equivalency Factors for Tetrodotoxin Analogues Determined with Automated Patch Clamp on Voltage-Gated Sodium Channels in Neuro-2a Cells Journal Article Journal of Agricultural and Food Chemistry, 72 (32), pp. 18192–18200, 2024, ISSN: 0021-8561, 1520-5118. |
Mass mortality of the invasive echinoid Diadema setosum (Leske, 1778) in Crete, East Mediterranean Sea Journal Article Mediterranean Marine Science, 25 (2), pp. 480–483, 2024, ISSN: 1791-6763, 1108-393X. |
Comparison of ovarian mRNA expression levels in wild and hatchery-produced greater amberjack Seriola dumerili Journal Article Scientific Reports, 14 (1), pp. 18034, 2024, ISSN: 2045-2322. |