Toxicology and biomarkers
Key research areas
Oxidative stress, Metabolism of chemicals, Gene expression, Enzymes activities-nutrient interactions, Non-invasive biomarkers
- A cell culture & bioassay room of biohazard level II, where transfected mammalian cells or fish cell lines are cultured for cytotoxicity & toxicity assays. It is mainly equipped with a biosafety cabinet, a humid CO2 incubator, an incubator, a water bath, a bench centrifuge, a refrigerator, a freezer (-20oC), a deep freeze (-80oC), a cell counter, an inverted microscope, a liquid N2 container, a sterilizer, etc.
- Two biomarker assays rooms (I & II), where biochemical biomarker responses (enzyme activities), and gene expression and protein analysis are performed. They are equipped with a chemical hood, a laminar flow hood, tissue extraction homogenisers (polytron & glass Teflon), a multi-cell micro-plate spectro-fluo photometer, a luminometer, two bench top refrigerated centrifuges, a peristaltic pump, a refrigerator, a freezer (-20oC), an ultra-freezer (-80oC), a bench top pH meter, electrophoresis apparatus for RNA/DNA & proteins (1D & 2D), DGGE & Western plotting, a thermo-cycler (PCR), a gel documentation imager system, a genetic analyser, etc.