SparComp

Title: Comparative transcriptomics in four sparid species using next-generation sequencing and comparative mapping based on available genetic information from model fish species

Funding Source: NSRF Excellence

Budget IMBBC: 400,000€

Start / End Date: 2012 - 2015

Web site URL:

Project Progress: 100%

Principal Investigator:

Costas Tsigenopoulos

Project Members:

Research Directions:

Reproduction and physiology

Functional and Comparative genomics

Description:

The project aimed to report the functional annotation and comparative analyses of transcriptome data sets using Next Generation Sequencing (NGS) technologies for four recently reared sparids with high commercial interest but very limited sequence information available: the common sea bream (Pagrus pagrus), the sharpsnout sea bream (Diplodus puntazzo), the common Pandora (Pagellus erythrinus) and the common dentex (Dentex dentex). The spawning kinetics of the four sparids was monitored using microsatellite genotyping to determine the number of spawning events per female and male participation. Furthermore, a linkage map was generated for each species using F1 full-sib families coming from wild broodstock and the ddRAD sequencing methodology