2006 |
Agulleiro, M J; Anguis, V; Cañavate, J P; Martínez-Rodríguez, G; Mylonas, C C; Cerdà, J Aquaculture, 257 (1-4), pp. 511–524, 2006, ISSN: 00448486. @article{agulleiro_induction_2006, title = {Induction of spawning of captive-reared Senegal sole (Solea senegalensis) using different administration methods for gonadotropin-releasing hormone agonist}, author = {M J Agulleiro and V Anguis and J P Cañavate and G Martínez-Rodríguez and C C Mylonas and J Cerdà}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33744478945&doi=10.1016%2fj.aquaculture.2006.02.001&partnerID=40&md5=2c10889e6bad052483983bc9ef891f55}, doi = {10.1016/j.aquaculture.2006.02.001}, issn = {00448486}, year = {2006}, date = {2006-01-01}, journal = {Aquaculture}, volume = {257}, number = {1-4}, pages = {511--524}, abstract = {Captive-reared Senegal sole were treated with intramuscular injection or sustained-release implants of gonadotropin-releasing hormone agonist [D-Ala6, Pro9, NEt] GnRHa (GnRHa) to induce spermiation and spawning. Fish were treated with GnRHa during spring (April-May) or autumn (October), the natural spawning seasons known to occur in wild Senegal sole. During spring, females injected with a dose of 5 μg GnRHa kg- 1 three times a week, or treated with a single GnRHa-loaded implant (50 μg kg- 1) showed multiple ovulations and spawns within a period of approximately 30 days. Plasma levels of 17β-estradiol (E2) were elevated at 7 days post-treatment, preceding the spawns with the highest fecundity, concomitant with a decrease in the levels of plasma testosterone (T). During autumn, however, females changed their responsiveness to GnRHa treatment, a dose of 5 μg kg- 1 being ineffective in inducing spawning, while a dose of 1 μg kg- 1 induced a few spawns. Accordingly, plasma E2 levels showed a small increase after injection of 1 μg kg- 1 GnRHa. In contrast to females, GnRHa injection or implantation in males was ineffective in enhancing spermiation or milt production, regardless of the season when the treatments were applied. However, GnRHa treatment in males increased plasma levels of T and 11-ketotestosterone (11-KT) during spring, while in autumn the levels of plasma androgens decreased after GnRHa injection. These results show that administration of GnRHa during spring either by injection or sustained-release implants induces multiple spawns in captive Senegal sole, although these treatments were ineffective in inducing or enhancing sperm production in males. © 2006 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Captive-reared Senegal sole were treated with intramuscular injection or sustained-release implants of gonadotropin-releasing hormone agonist [D-Ala6, Pro9, NEt] GnRHa (GnRHa) to induce spermiation and spawning. Fish were treated with GnRHa during spring (April-May) or autumn (October), the natural spawning seasons known to occur in wild Senegal sole. During spring, females injected with a dose of 5 μg GnRHa kg- 1 three times a week, or treated with a single GnRHa-loaded implant (50 μg kg- 1) showed multiple ovulations and spawns within a period of approximately 30 days. Plasma levels of 17β-estradiol (E2) were elevated at 7 days post-treatment, preceding the spawns with the highest fecundity, concomitant with a decrease in the levels of plasma testosterone (T). During autumn, however, females changed their responsiveness to GnRHa treatment, a dose of 5 μg kg- 1 being ineffective in inducing spawning, while a dose of 1 μg kg- 1 induced a few spawns. Accordingly, plasma E2 levels showed a small increase after injection of 1 μg kg- 1 GnRHa. In contrast to females, GnRHa injection or implantation in males was ineffective in enhancing spermiation or milt production, regardless of the season when the treatments were applied. However, GnRHa treatment in males increased plasma levels of T and 11-ketotestosterone (11-KT) during spring, while in autumn the levels of plasma androgens decreased after GnRHa injection. These results show that administration of GnRHa during spring either by injection or sustained-release implants induces multiple spawns in captive Senegal sole, although these treatments were ineffective in inducing or enhancing sperm production in males. © 2006 Elsevier B.V. All rights reserved. |
Papageorgiou, Nafsika; Arvanitidis, C; Eleftheriou, A Macrofaunal Biodiversity of the Mediterranean Sandy Beach Environment Inproceedings The Mediterranean Coastal Areas from Watershed to the Sea : Interctions and Changes : Proceedings of the Medcore International Conference, pp. 263–274, Firenze, ITALY, 2006, (Publication Title: The Mediterranean Coastal Areas from Watershed to the Sea : Interctions and Changes : Proceedings of the Medcore International Conference Type: Conference Paper). @inproceedings{papageorgiou_macrofaunal_2006, title = {Macrofaunal Biodiversity of the Mediterranean Sandy Beach Environment}, author = {Nafsika Papageorgiou and C Arvanitidis and A Eleftheriou}, year = {2006}, date = {2006-01-01}, booktitle = {The Mediterranean Coastal Areas from Watershed to the Sea : Interctions and Changes : Proceedings of the Medcore International Conference}, pages = {263--274}, address = {Firenze, ITALY}, abstract = {Information collected both from sampling activities in the Eastern and Western Mediterranean sandy beaches as well as from the literature has reveled: (i) Severe fluctuations in the values of the abiotic data, consistent with the “multicausal environmental severity hypothesis”; (ii) A tentatively defined zoogeocline from the Eastern to the Western Mediterranean; (iii) Many of the sandy beaches show higher than expected Taxonomic Distinctness values, a fact which is indicative of the less scientific effort spent, and which results in many oligo- or monotypic taxa; (iv) The most plausible results were derived from the taxon of polychaetes, which have also proved to be a successful indicator taxon in other transitional Mediterranean ecosystems.}, note = {Publication Title: The Mediterranean Coastal Areas from Watershed to the Sea : Interctions and Changes : Proceedings of the Medcore International Conference Type: Conference Paper}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } Information collected both from sampling activities in the Eastern and Western Mediterranean sandy beaches as well as from the literature has reveled: (i) Severe fluctuations in the values of the abiotic data, consistent with the “multicausal environmental severity hypothesis”; (ii) A tentatively defined zoogeocline from the Eastern to the Western Mediterranean; (iii) Many of the sandy beaches show higher than expected Taxonomic Distinctness values, a fact which is indicative of the less scientific effort spent, and which results in many oligo- or monotypic taxa; (iv) The most plausible results were derived from the taxon of polychaetes, which have also proved to be a successful indicator taxon in other transitional Mediterranean ecosystems. |
Claus, S; Arvanitidis, C; Fleddum, A; Berghe, Vanden E MarBEF theme 1: global patterns of marine biodiversity across ecosystems. Progress with theme I data analysis activities Journal Article MarBEF Newsletter, 4 , pp. 3–4, 2006. @article{claus_marbef_2006, title = {MarBEF theme 1: global patterns of marine biodiversity across ecosystems. Progress with theme I data analysis activities}, author = {S Claus and C Arvanitidis and A Fleddum and E Vanden Berghe}, url = {http://www.marinespecies.org/imis.php?module=ref&refid=98966}, year = {2006}, date = {2006-01-01}, journal = {MarBEF Newsletter}, volume = {4}, pages = {3--4}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Arvanitidis, C; Valavanis, V D; Eleftheriou, A; Costello, M J; Faulwetter, S; Gotsis, P; Kitsos, M S; Kirmtzoglou, I; Zenetos, A; Petrov, A; Galil, B; Papageorgiou, N MedOBIS: Biogeographic information system for the eastern Mediterranean and Black Sea Journal Article Marine Ecology Progress Series, 316 , pp. 225–230, 2006, ISSN: 01718630. @article{arvanitidis_medobis_2006, title = {MedOBIS: Biogeographic information system for the eastern Mediterranean and Black Sea}, author = {C Arvanitidis and V D Valavanis and A Eleftheriou and M J Costello and S Faulwetter and P Gotsis and M S Kitsos and I Kirmtzoglou and A Zenetos and A Petrov and B Galil and N Papageorgiou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33746589895&doi=10.3354%2fmeps316225&partnerID=40&md5=e72c488455d8d116da5aae7d7faf91b4}, doi = {10.3354/meps316225}, issn = {01718630}, year = {2006}, date = {2006-01-01}, journal = {Marine Ecology Progress Series}, volume = {316}, pages = {225--230}, abstract = {Recent online initiatives in sharing marine biological data, such as the European Register of Marine Species (ERMS) and the Ocean Biogeographic Information System (OBIS), identified gaps in data from the eastern Mediterranean and Black Sea. Such data are now being collected, formatted and disseminated by MedOBIS (the Mediterranean Ocean Biogeographic Information System) initiative involving Greece, the Ukraine and Israel (test version available at: www.medobis.org). The aim is to develop a taxon-based biogeography database and online data server with links to survey and satellite environmental data. MedOBIS is currently undergoing 4 stages of development, namely, data assembly, formatting, analysis and dissemination. The primary features of the MedOBIS application are its offline GIS (Geographic Information Systems) data formatting capabilities and its online Java- and JavaScript-enabling data server with taxon-based search, mapping and data down-loading capabilities. It is an independent source of biological and environmental data, as well as an online GIS tool designed to facilitate access to historical and current data by marine researchers. As more data become available and are inserted into the system, MedOBIS will function as the eastern Mediterranean and Black Sea node of EurOBIS (the European node of the international OBIS initiative, part of the 'Census of Marine Life'). © Inter-Research 2006.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Recent online initiatives in sharing marine biological data, such as the European Register of Marine Species (ERMS) and the Ocean Biogeographic Information System (OBIS), identified gaps in data from the eastern Mediterranean and Black Sea. Such data are now being collected, formatted and disseminated by MedOBIS (the Mediterranean Ocean Biogeographic Information System) initiative involving Greece, the Ukraine and Israel (test version available at: www.medobis.org). The aim is to develop a taxon-based biogeography database and online data server with links to survey and satellite environmental data. MedOBIS is currently undergoing 4 stages of development, namely, data assembly, formatting, analysis and dissemination. The primary features of the MedOBIS application are its offline GIS (Geographic Information Systems) data formatting capabilities and its online Java- and JavaScript-enabling data server with taxon-based search, mapping and data down-loading capabilities. It is an independent source of biological and environmental data, as well as an online GIS tool designed to facilitate access to historical and current data by marine researchers. As more data become available and are inserted into the system, MedOBIS will function as the eastern Mediterranean and Black Sea node of EurOBIS (the European node of the international OBIS initiative, part of the 'Census of Marine Life'). © Inter-Research 2006. |
Polymenakou, P N; Tselepides, A; Stephanou, E G; Bertilsson, S Microbial “life markers” in the deep hypersaline anoxic basins (DHABs) of the South Ionian Sea Inproceedings Thessaloniki, Greece, 2006, (Publication Title: 8th Pan-Hellenic Symposium of Oceanography & Fisheries Type: poster). @inproceedings{polymenakou_microbial_2006, title = {Microbial “life markers” in the deep hypersaline anoxic basins (DHABs) of the South Ionian Sea}, author = {P N Polymenakou and A Tselepides and E G Stephanou and S Bertilsson}, year = {2006}, date = {2006-01-01}, address = {Thessaloniki, Greece}, note = {Publication Title: 8th Pan-Hellenic Symposium of Oceanography & Fisheries Type: poster}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Makridis, P; Costa, R A; Dinis, M T Aquaculture, 255 , pp. 76 – 81, 2006. @article{makridis_microbial_2006, title = {Microbial conditions and antimicrobial activity in cultures of two microalgae species, Tetraselmis chuii and Chlorella minutissima, and effect on bacterial load of enriched Artemia metanauplii}, author = {P Makridis and R A Costa and M T Dinis}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33646882858&partnerID=40&md5=8bede327c33d2e1919b675bedd6046e1}, year = {2006}, date = {2006-01-01}, journal = {Aquaculture}, volume = {255}, pages = {76 -- 81}, abstract = {The microbial conditions and antimicrobial activity were determined in batch cultures of two microalgae species, Tetraselmis chuii and Chlorella minutissima. The number of bacteria associated with the microalgae cultures showed an exponential growth 2, 10, and 16 days after inoculation, and they were higher in T. chuii in all three sampling points compared with C. minutissima. No presumptive Vibrio strains were observed in any of the samples, as measured by the growth on TCBS agar. A total of 17 and 30 bacterial strains were isolated from C. minutissima and T. chuii, respectively. A high percentage of Gram-positive strains was detected among the bacterial strains isolated, as Gram-positive strains constituted 82% (14 / 17) and 73% (22 / 30) of the total numbers of isolates in C. minutissima and T. chuii, respectively. The isolated bacteria were screened in vitro for inhibition against two pathogenic strains, and nine of the 34 strains tested (26%) showed inhibition in vitro against either Photobacterium damselae, susp. piscicida or Vibrio anguillarum. Incubation of enriched Artemia in cultures of the two microalgae for 30 min resulted in a significant decrease of the bacterial load in Artemia (P textless 0.05), and a significant decrease of the level of presumptive Vibrio in Artemia homogenates (P textless 0.05). The results of this study demonstrate a simple and practical approach to decrease the microbial load and at the same time reduce the percentage of Vibrio among the bacteria associated with enriched Artemia. © 2005 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The microbial conditions and antimicrobial activity were determined in batch cultures of two microalgae species, Tetraselmis chuii and Chlorella minutissima. The number of bacteria associated with the microalgae cultures showed an exponential growth 2, 10, and 16 days after inoculation, and they were higher in T. chuii in all three sampling points compared with C. minutissima. No presumptive Vibrio strains were observed in any of the samples, as measured by the growth on TCBS agar. A total of 17 and 30 bacterial strains were isolated from C. minutissima and T. chuii, respectively. A high percentage of Gram-positive strains was detected among the bacterial strains isolated, as Gram-positive strains constituted 82% (14 / 17) and 73% (22 / 30) of the total numbers of isolates in C. minutissima and T. chuii, respectively. The isolated bacteria were screened in vitro for inhibition against two pathogenic strains, and nine of the 34 strains tested (26%) showed inhibition in vitro against either Photobacterium damselae, susp. piscicida or Vibrio anguillarum. Incubation of enriched Artemia in cultures of the two microalgae for 30 min resulted in a significant decrease of the bacterial load in Artemia (P textless 0.05), and a significant decrease of the level of presumptive Vibrio in Artemia homogenates (P textless 0.05). The results of this study demonstrate a simple and practical approach to decrease the microbial load and at the same time reduce the percentage of Vibrio among the bacteria associated with enriched Artemia. © 2005 Elsevier B.V. All rights reserved. |
Magoulas, Antonios; Castilho, R; Caetano, S; Marcato, S; Patarnello, T Mol Phylogenet Evol, 39 (3), pp. 734–46, 2006. @article{magoulas_mitochondrial_2006, title = {Mitochondrial DNA reveals a mosaic pattern of phylogeographical structure in Atlantic and Mediterranean populations of anchovy (Engraulis encrasicolus)}, author = {Antonios Magoulas and R Castilho and S Caetano and S Marcato and T Patarnello}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16515866}, year = {2006}, date = {2006-01-01}, journal = {Mol Phylogenet Evol}, volume = {39}, number = {3}, pages = {734--46}, abstract = {This study extends the geographic coverage of a previous study of mitochondrial DNA restriction fragment length polymorphism in European anchovy. Both studies together include 24 samples representing 17 localities extending from the Black Sea, through the Mediterranean Sea to the eastern Atlantic as far south as Dakar, Senegal. Eighty-eight haplotypes define two clades (A and B) separated by 3.2% sequence divergence. Clade A has a star-like genealogy indicative of a recent population expansion. Clade B has a more complex genealogy, consisting of several haplotypes at intermediate frequencies. The distributions of these clades consist of a mosaic with abrupt changes between some areas and gradients between other areas. Clade A predominates the Black and Aegean seas, but is present throughout the Mediterranean. Unexpectedly, new data show that clade A is also at a high frequency in the Atlantic, from Portugal to at least Senegal. Overall, the level of genetic differentiation among populations is high (F(ST)=0.148, ptextless0.0001), with the greatest differences between basins. AMOVA reveals four main geographical groups: Atlantic, central Mediterranean, Aegean Sea, and Black Sea. Mismatch distribution clearly indicates historical bottleneck and population expansion for clade A, while for clade B such evidence is equivocal. This difference may reflect a range expansion for both clades, but with higher gene flow (Nm values) between demes for clade A. Both contemporary and historical processes are important in shaping the complex genetic population structure of European anchovy.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study extends the geographic coverage of a previous study of mitochondrial DNA restriction fragment length polymorphism in European anchovy. Both studies together include 24 samples representing 17 localities extending from the Black Sea, through the Mediterranean Sea to the eastern Atlantic as far south as Dakar, Senegal. Eighty-eight haplotypes define two clades (A and B) separated by 3.2% sequence divergence. Clade A has a star-like genealogy indicative of a recent population expansion. Clade B has a more complex genealogy, consisting of several haplotypes at intermediate frequencies. The distributions of these clades consist of a mosaic with abrupt changes between some areas and gradients between other areas. Clade A predominates the Black and Aegean seas, but is present throughout the Mediterranean. Unexpectedly, new data show that clade A is also at a high frequency in the Atlantic, from Portugal to at least Senegal. Overall, the level of genetic differentiation among populations is high (F(ST)=0.148, ptextless0.0001), with the greatest differences between basins. AMOVA reveals four main geographical groups: Atlantic, central Mediterranean, Aegean Sea, and Black Sea. Mismatch distribution clearly indicates historical bottleneck and population expansion for clade A, while for clade B such evidence is equivocal. This difference may reflect a range expansion for both clades, but with higher gene flow (Nm values) between demes for clade A. Both contemporary and historical processes are important in shaping the complex genetic population structure of European anchovy. |
Koulouri, P; Dounas, C; Arvanitidis, C; Koutsoubas, D; Eleftheriou, A Molluscan diversity along a Mediterranean soft bottom sublittoral ecotone Journal Article Scientia Marina, 70 (4), pp. 573–583, 2006, ISSN: 02148358, (Publisher: CSIC Consejo Superior de Investigaciones Cientificas 2). @article{koulouri_molluscan_2006, title = {Molluscan diversity along a Mediterranean soft bottom sublittoral ecotone}, author = {P Koulouri and C Dounas and C Arvanitidis and D Koutsoubas and A Eleftheriou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33846020553&doi=10.3989%2fscimar.2006.70n4573&partnerID=40&md5=7281e64db760bd5bee1e6b6611feef33}, doi = {10.3989/scimar.2006.70n4573}, issn = {02148358}, year = {2006}, date = {2006-01-01}, journal = {Scientia Marina}, volume = {70}, number = {4}, pages = {573--583}, abstract = {Molluscan diversity associated with sublittoral soft-bottoms was studied in Heraklion Bay as part of a long-term multidisciplinary research programme in the coastal environment of northern Crete (eastern Mediterranean). Quantitative benthic samples were collected in a pilot survey from a grid of 28 stations, followed by a seasonal sampling carried out over a seven- station transect located from 10 to 40 m depth. Univariate and multivariate analyses indicated the presence of a clear zonation along the depth gradient for the molluscan fauna and three main associations were identified in bathymetric zones parallel to the coastline. The structure of the local molluscan communities revealed the existence of an extended transition zone (ecotone) between a shallow sandy association and a deeper muddy one and a functional pattern, based on the feeding guilds, was evident along the environmental gradient. Application of graphical techniques, by using molluscan taxocoenosis, indicated a rather naturally disturbed habitat. Beta-diversity measures indicated different seasonal community adaptations to the prevailing environmental conditions. Results derived from this study revealed that beta-diversity may be a good descriptor for the assessment of the strength and breadth of sublittoral transition zones along environmental gradients.}, note = {Publisher: CSIC Consejo Superior de Investigaciones Cientificas 2}, keywords = {}, pubstate = {published}, tppubtype = {article} } Molluscan diversity associated with sublittoral soft-bottoms was studied in Heraklion Bay as part of a long-term multidisciplinary research programme in the coastal environment of northern Crete (eastern Mediterranean). Quantitative benthic samples were collected in a pilot survey from a grid of 28 stations, followed by a seasonal sampling carried out over a seven- station transect located from 10 to 40 m depth. Univariate and multivariate analyses indicated the presence of a clear zonation along the depth gradient for the molluscan fauna and three main associations were identified in bathymetric zones parallel to the coastline. The structure of the local molluscan communities revealed the existence of an extended transition zone (ecotone) between a shallow sandy association and a deeper muddy one and a functional pattern, based on the feeding guilds, was evident along the environmental gradient. Application of graphical techniques, by using molluscan taxocoenosis, indicated a rather naturally disturbed habitat. Beta-diversity measures indicated different seasonal community adaptations to the prevailing environmental conditions. Results derived from this study revealed that beta-diversity may be a good descriptor for the assessment of the strength and breadth of sublittoral transition zones along environmental gradients. |
Katharios, P; Garaffo, M; Sarter, K; Poulou, Athanasso F; Mylonas, C C; Divanach, P Mortality of steroid-treated sharpsnout sea bream Diplodus puntazzo caused by Ceratomyxa diplodae (Protozoa: Myxosporea). Inproceedings Fifth International Symposium on Aquatic Animal Health, pp. 148, San Francisco, U.S., 2006. @inproceedings{katharios_mortality_2006, title = {Mortality of steroid-treated sharpsnout sea bream Diplodus puntazzo caused by Ceratomyxa diplodae (Protozoa: Myxosporea).}, author = {P Katharios and M Garaffo and K Sarter and F Athanasso Poulou and C C Mylonas and P Divanach}, year = {2006}, date = {2006-01-01}, booktitle = {Fifth International Symposium on Aquatic Animal Health}, pages = {148}, address = {San Francisco, U.S.}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Papageorgiou, N; Arvanitidis, C; Eleftheriou, A Multicausal environmental severity: A flexible framework for microtidal sandy beaches and the role of polychaetes as an indicator taxon Journal Article Estuarine, Coastal and Shelf Science, 70 (4), pp. 643–653, 2006, ISSN: 02727714. @article{papageorgiou_multicausal_2006, title = {Multicausal environmental severity: A flexible framework for microtidal sandy beaches and the role of polychaetes as an indicator taxon}, author = {N Papageorgiou and C Arvanitidis and A Eleftheriou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33751414872&doi=10.1016%2fj.ecss.2005.11.033&partnerID=40&md5=4604b4489092bbe973f264257fe4cf43}, doi = {10.1016/j.ecss.2005.11.033}, issn = {02727714}, year = {2006}, date = {2006-01-01}, journal = {Estuarine, Coastal and Shelf Science}, volume = {70}, number = {4}, pages = {643--653}, abstract = {The aim of this paper is to study the macrofaunal community dynamics and the biological-environmental interactions in the mid- and sublittoral ecosystems of the microtidal Mediterranean sandy shores. Four sandy beaches, three on the island of Crete and one on the northwest coast of Italy were selected to investigate the spatial and temporal changes in the community structure and the associated environmental variables. The littoral zone, which has not been adequately studied in the Eastern Mediterranean, presents special interest not only from the scientific point of view but also for practical reasons of ecological management. The multivariate techniques revealed that the community pattern of the sandy beach macrofauna is mainly spatial rather than temporal. There are pronounced differences in species composition and abundance of the macrofaunal assemblages of the mid- and sublittoral zone. The multicausal environmental severity hypothesis appears to be valid for the sandy beach macrofaunal communities of the Mediterranean. The abundance and composition of the macrofaunal assemblages are highly variable and are affected by the synergistic effects of many environmental variables. The polychaete taxonomic assemblage structure closely follows the macrofaunal community pattern. Differences between the two patterns may arise from the different responses that polychaetes may show to the environmental stress. © 2006 Elsevier Ltd. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The aim of this paper is to study the macrofaunal community dynamics and the biological-environmental interactions in the mid- and sublittoral ecosystems of the microtidal Mediterranean sandy shores. Four sandy beaches, three on the island of Crete and one on the northwest coast of Italy were selected to investigate the spatial and temporal changes in the community structure and the associated environmental variables. The littoral zone, which has not been adequately studied in the Eastern Mediterranean, presents special interest not only from the scientific point of view but also for practical reasons of ecological management. The multivariate techniques revealed that the community pattern of the sandy beach macrofauna is mainly spatial rather than temporal. There are pronounced differences in species composition and abundance of the macrofaunal assemblages of the mid- and sublittoral zone. The multicausal environmental severity hypothesis appears to be valid for the sandy beach macrofaunal communities of the Mediterranean. The abundance and composition of the macrofaunal assemblages are highly variable and are affected by the synergistic effects of many environmental variables. The polychaete taxonomic assemblage structure closely follows the macrofaunal community pattern. Differences between the two patterns may arise from the different responses that polychaetes may show to the environmental stress. © 2006 Elsevier Ltd. All rights reserved. |
Zaiss, M M; Papadakis, I E; Maingot, E; Divanach, P; Mylonas, C C Ontogeny of the digestive tract in shi drum (Umbrina cirrosa L.) reared using the mesocosm larval rearing system Journal Article Aquaculture, 260 (1-4), pp. 357–368, 2006, ISSN: 00448486. @article{zaiss_ontogeny_2006, title = {Ontogeny of the digestive tract in shi drum (Umbrina cirrosa L.) reared using the mesocosm larval rearing system}, author = {M M Zaiss and I E Papadakis and E Maingot and P Divanach and C C Mylonas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33747800319&doi=10.1016%2fj.aquaculture.2006.06.001&partnerID=40&md5=4f7c6eb4f3932426b7e5fb62cafa6f95}, doi = {10.1016/j.aquaculture.2006.06.001}, issn = {00448486}, year = {2006}, date = {2006-01-01}, journal = {Aquaculture}, volume = {260}, number = {1-4}, pages = {357--368}, abstract = {Histological changes of the digestive tract were studied in shi drum (Umbrina cirrosa) from hatching until 41 days post hatching (dph), when the fry had a mean (±S.D.) total length (TL) of 32 ± 2 mm and wet weight (WW) of 0.42 ± 0.07 g. Larvae were reared using the mesocosm technique, the most natural among commercially employed rearing methods for marine larvae. Shi drum opened their mouth at 2 dph (2.78 ± 0.09 mm TL), at which time 90% of the larvae already had an inflated swim bladder. The differentiation of the digestive tract into buccopharynx, esophagus, and anterior and posterior intestine was completed by 3 dph (2.82 ± 0.07 mm TL), 1 day after the onset of exogenous feeding. The alimentary canal started coiling and formed its first loop at 2 dph, while the pancreas and liver were differentiated at 3 dph. Yolk sac reserves lasted until 7 dph (4.3 ± 0.1 mm TL), suggesting a brief period of endogenous and exogenous feeding. The first esophageal goblet cells appeared at 7 dph containing acid mucins and at 8 dph taste buds appeared on the buccopharyngeal epithelium. The stomach was morphologically differentiated at 9 dph (5.5 ± 0.1 mm TL) when gastric glands became abundant in the cardiac region, and the first pyloric caeca appeared at 14 dph (10.1 ± 0.9 mm TL). Supranuclear eosinophilic vacuoles were observed in the posterior intestine between 3 and 11 dph (6.3 ± 0.9 mm TL). Their number decreased as the stomach differentiated, suggesting a change in the protein digestion mechanism. The results of the study suggest a rapid development of shi drum and its digestive system and underline the possibility of weaning larvae to artificial feed even earlier than the 12 dph employed in the present study. © 2006 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Histological changes of the digestive tract were studied in shi drum (Umbrina cirrosa) from hatching until 41 days post hatching (dph), when the fry had a mean (±S.D.) total length (TL) of 32 ± 2 mm and wet weight (WW) of 0.42 ± 0.07 g. Larvae were reared using the mesocosm technique, the most natural among commercially employed rearing methods for marine larvae. Shi drum opened their mouth at 2 dph (2.78 ± 0.09 mm TL), at which time 90% of the larvae already had an inflated swim bladder. The differentiation of the digestive tract into buccopharynx, esophagus, and anterior and posterior intestine was completed by 3 dph (2.82 ± 0.07 mm TL), 1 day after the onset of exogenous feeding. The alimentary canal started coiling and formed its first loop at 2 dph, while the pancreas and liver were differentiated at 3 dph. Yolk sac reserves lasted until 7 dph (4.3 ± 0.1 mm TL), suggesting a brief period of endogenous and exogenous feeding. The first esophageal goblet cells appeared at 7 dph containing acid mucins and at 8 dph taste buds appeared on the buccopharyngeal epithelium. The stomach was morphologically differentiated at 9 dph (5.5 ± 0.1 mm TL) when gastric glands became abundant in the cardiac region, and the first pyloric caeca appeared at 14 dph (10.1 ± 0.9 mm TL). Supranuclear eosinophilic vacuoles were observed in the posterior intestine between 3 and 11 dph (6.3 ± 0.9 mm TL). Their number decreased as the stomach differentiated, suggesting a change in the protein digestion mechanism. The results of the study suggest a rapid development of shi drum and its digestive system and underline the possibility of weaning larvae to artificial feed even earlier than the 12 dph employed in the present study. © 2006 Elsevier B.V. All rights reserved. |
Holmstrand, H; Gadomski, D; Mandalakis, M; Tysklind, M; Irvine, R; Andersson, P; Gustafsson, Ö Origin of PCDDs in Ball Clay assessed with compound-specific chlorine isotope analysis and radiocarbon dating Journal Article Environmental Science and Technology, 40 (12), pp. 3730–3735, 2006, ISSN: 0013936X. @article{holmstrand_origin_2006, title = {Origin of PCDDs in Ball Clay assessed with compound-specific chlorine isotope analysis and radiocarbon dating}, author = {H Holmstrand and D Gadomski and M Mandalakis and M Tysklind and R Irvine and P Andersson and Ö Gustafsson}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33745152689&doi=10.1021%2fes0602142&partnerID=40&md5=bd5ef3dfec20a26979baa18cc19fc427}, doi = {10.1021/es0602142}, issn = {0013936X}, year = {2006}, date = {2006-01-01}, journal = {Environmental Science and Technology}, volume = {40}, number = {12}, pages = {3730--3735}, abstract = {Polychlorinated dibenzo-p-dioxins (PCDDs) of high concentrations in a ball clay deposit from the Mississippi Embayment were found to be consistent with a natural abiotic and non-pyrogenic origin by investigation with bulk radiocarbon analysis, compound-specific chlorine isotope analysis (CSIA- δ37Cl) of octachlorodibenzo-p-dioxin (OCDD), and black carbon (BC) analysis. The conventional radiocarbon date of total organic carbon from a depth of approximately 10 m in three parallel cores ranged from 14 700 years to >48 000 years, indicating that the strata with elevated levels of PCDDs have remained isolated from recent anthropogenic input in these >40 Ma old clay sediments. The CSIA-δ37Cl of OCDD yielded a δ37Cl of -0.2%, which is significantly higher than the postulated range for biotic chlorination by chloroperoxidase enzymes, -11 to -10‰, and falls within the known range for abiotic organochlorines, -6 to +3‰. The absence of correlations between concentrations of PCDDs and corresponding pyrogenic black carbon (BC), together with estimations of BC sorptive loadings and the absence of polychlorinated dibenzofurans (PCDFs), suggest that vegetation fires did not form these ball-clay PCDDs. Results from this study indicate that the high levels of the toxic and carcinogenic PCDDs found in kaolinite-bearing clays may result from natural abiotic formation via in situ surface-promoted reactions on the clay mineral, including a so-far unknown organic precursor, rather than being the result of anthropogenic contamination. © 2006 American Chemical Society.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Polychlorinated dibenzo-p-dioxins (PCDDs) of high concentrations in a ball clay deposit from the Mississippi Embayment were found to be consistent with a natural abiotic and non-pyrogenic origin by investigation with bulk radiocarbon analysis, compound-specific chlorine isotope analysis (CSIA- δ37Cl) of octachlorodibenzo-p-dioxin (OCDD), and black carbon (BC) analysis. The conventional radiocarbon date of total organic carbon from a depth of approximately 10 m in three parallel cores ranged from 14 700 years to >48 000 years, indicating that the strata with elevated levels of PCDDs have remained isolated from recent anthropogenic input in these >40 Ma old clay sediments. The CSIA-δ37Cl of OCDD yielded a δ37Cl of -0.2%, which is significantly higher than the postulated range for biotic chlorination by chloroperoxidase enzymes, -11 to -10‰, and falls within the known range for abiotic organochlorines, -6 to +3‰. The absence of correlations between concentrations of PCDDs and corresponding pyrogenic black carbon (BC), together with estimations of BC sorptive loadings and the absence of polychlorinated dibenzofurans (PCDFs), suggest that vegetation fires did not form these ball-clay PCDDs. Results from this study indicate that the high levels of the toxic and carcinogenic PCDDs found in kaolinite-bearing clays may result from natural abiotic formation via in situ surface-promoted reactions on the clay mineral, including a so-far unknown organic precursor, rather than being the result of anthropogenic contamination. © 2006 American Chemical Society. |
Rigos, G; Nengas, I; Alexis, M Oxytetracycline (OTC) uptake following bath treatment in gilthead sea bream (Sparus aurata) Journal Article Aquaculture, 261 , pp. 1151 – 1155, 2006. @article{rigos_oxytetracycline_2006, title = {Oxytetracycline (OTC) uptake following bath treatment in gilthead sea bream (Sparus aurata)}, author = {G Rigos and I Nengas and M Alexis}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33751440278&partnerID=40&md5=e595050cb25e5846e346f95e8827b71d}, year = {2006}, date = {2006-01-01}, journal = {Aquaculture}, volume = {261}, pages = {1151 -- 1155}, abstract = {The uptake and elimination profile of oxytetracycline (OTC) following a prolong bath treatment in gilthhead sea bream (Sparus aurata) were investigated in this study. The bath experiment was carried out using a OTC concentration of 50 μg/ml for 24 h at 17-18 °C water temperature. Plasma and muscle fish samples were analysed at 1, 3, 6 and 24 h during and at 1, 2, 3, 4 and 6 d following the bath. Detectable OTC levels were revealed only at the end of bath treatment (24 h) in examined tissues of gilthead sea bream, where drug concentration was measured to be as low as 0.096 and 0.047 μg/g or ml in muscle plus skin and plasma, respectively. The findings of the present study indicate that OTC bath treatment under this dosage schedule is unlikely to confront systemic bacterial infections. © 2006 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The uptake and elimination profile of oxytetracycline (OTC) following a prolong bath treatment in gilthhead sea bream (Sparus aurata) were investigated in this study. The bath experiment was carried out using a OTC concentration of 50 μg/ml for 24 h at 17-18 °C water temperature. Plasma and muscle fish samples were analysed at 1, 3, 6 and 24 h during and at 1, 2, 3, 4 and 6 d following the bath. Detectable OTC levels were revealed only at the end of bath treatment (24 h) in examined tissues of gilthead sea bream, where drug concentration was measured to be as low as 0.096 and 0.047 μg/g or ml in muscle plus skin and plasma, respectively. The findings of the present study indicate that OTC bath treatment under this dosage schedule is unlikely to confront systemic bacterial infections. © 2006 Elsevier B.V. All rights reserved. |
Katharios, P; Hayward, C; Papandroulakis, N; Divanach, P Pathology of Lamellodiscus spp. (Monogenea) parasitizing the gills of sharpsnout seabream and preliminary results of formalin treatment Journal Article Bulletin of the European Association of Fish Pathologists, 26 (5), pp. 196–201, 2006, ISSN: 01080288. @article{katharios_pathology_2006, title = {Pathology of Lamellodiscus spp. (Monogenea) parasitizing the gills of sharpsnout seabream and preliminary results of formalin treatment}, author = {P Katharios and C Hayward and N Papandroulakis and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33845670054&partnerID=40&md5=09814c007ac1f9256a311f1d53bc1f34}, issn = {01080288}, year = {2006}, date = {2006-01-01}, journal = {Bulletin of the European Association of Fish Pathologists}, volume = {26}, number = {5}, pages = {196--201}, abstract = {This report describes the pathological effects Lamellodiscus spp. on the gills of the sharpsnout seabream, Diplodus puntazzo and the efficacy of formalin as a treatment. Two species of Lamellodiscus were identified: Lamellodiscus ergensi and L. bidens. The attachment of parasites caused destruction, fusion and hyperplasia of the gill filaments. Formalin was effective in removing the worms when applied as a bath treatment at a concentration of 200mg/ml for one hour.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This report describes the pathological effects Lamellodiscus spp. on the gills of the sharpsnout seabream, Diplodus puntazzo and the efficacy of formalin as a treatment. Two species of Lamellodiscus were identified: Lamellodiscus ergensi and L. bidens. The attachment of parasites caused destruction, fusion and hyperplasia of the gill filaments. Formalin was effective in removing the worms when applied as a bath treatment at a concentration of 200mg/ml for one hour. |
Sarter, K; Papadaki, M; Zanuy, S; Mylonas, C C Aquaculture, 256 (1-4), pp. 443–456, 2006, ISSN: 00448486. @article{sarter_permanent_2006, title = {Permanent sex inversion in 1-year-old juveniles of the protogynous dusky grouper (Epinephelus marginatus) using controlled-release 17α-methyltestosterone implants}, author = {K Sarter and M Papadaki and S Zanuy and C C Mylonas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33646896490&doi=10.1016%2fj.aquaculture.2006.01.034&partnerID=40&md5=fae7d76571a7060e8182bf08f6c00e7e}, doi = {10.1016/j.aquaculture.2006.01.034}, issn = {00448486}, year = {2006}, date = {2006-01-01}, journal = {Aquaculture}, volume = {256}, number = {1-4}, pages = {443--456}, abstract = {Long-term treatments (April-June 2004) with 17α-methyltestosterone (MT) and/or human chorionic gonadotropin (hCG) were used to induce precocious sex change in 1-year-old juveniles (mean ± SD body weight of 131 ± 25 g) of the protogynous dusky grouper (Epinephelus marginatus). The MT treatment was given once a month using controlled-release implants (1.5 mg per implant, ∼11 mg kg- 1 body weight), whereas hCG was injected intramuscularly once a month (MT/hCG group) or every 2 weeks (hCG group). In vitro mean (± SEM) daily and total MT release from the implants was 22.6 ± 5.4 μg day- 1 implant- 1 and 686 ± 113 μg implant- 1, respectively. In vivo, plasma MT levels of European sea bas (Dicentrarchus labrax) used as model fish (203 ± 41 g body weight) and given a single MT implant were 18.5 ± 0.5 ng ml- 1 one day after implantation, declined to 9.6 ± 2.7 ng ml- 1 by day 11 and remained stable between 7.9 and 5.5 ng ml- 1 until day 32. Plasma MT levels were significantly elevated (2-way ANOVA, P < 0.01) in dusky grouper juveniles in response to the MT treatment, both 6 and 12 weeks after beginning the treatment. On the contrary, no significant differences (2-way ANOVA, P > 0.05) between MT/hCG-treated and control fish were observed in plasma testosterone (T) or 11-keto T (11-KT). Pituitary luteinizing hormone (LH) content was significantly lower in both the MT/hCG and hCG groups, compared to controls (2-way ANOVA, P < 0.001), while plasma LH was below detection limits in all treatments. At the onset of the experiment all but one fish were already differentiated into females, having ovaries with primary oocytes. No signs of vitellogenesis or sex inversion were observed in fish from the control and hCG groups during the study. On the contrary, the MT implants induced complete sex change after 12 weeks, with the gonads organized in lobules and cysts filled with germ cells at all stages of spermatogenesis, including spermatozoa. This sex inversion was permanent and fish underwent complete spermatogenesis again during the following year. The results demonstrate that the produced MT-implants, but not the hCG injections, were highly efficient in inducing both sex inversion and complete spermatogenesis in pre-pubertal dusky grouper at the completion of their second year in life. Production of male fish at such an early age may dramatically reduce by many years the time required for natural puberty, sex inversion and the production of sperm by dusky grouper reared under culture conditions. © 2006 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Long-term treatments (April-June 2004) with 17α-methyltestosterone (MT) and/or human chorionic gonadotropin (hCG) were used to induce precocious sex change in 1-year-old juveniles (mean ± SD body weight of 131 ± 25 g) of the protogynous dusky grouper (Epinephelus marginatus). The MT treatment was given once a month using controlled-release implants (1.5 mg per implant, ∼11 mg kg- 1 body weight), whereas hCG was injected intramuscularly once a month (MT/hCG group) or every 2 weeks (hCG group). In vitro mean (± SEM) daily and total MT release from the implants was 22.6 ± 5.4 μg day- 1 implant- 1 and 686 ± 113 μg implant- 1, respectively. In vivo, plasma MT levels of European sea bas (Dicentrarchus labrax) used as model fish (203 ± 41 g body weight) and given a single MT implant were 18.5 ± 0.5 ng ml- 1 one day after implantation, declined to 9.6 ± 2.7 ng ml- 1 by day 11 and remained stable between 7.9 and 5.5 ng ml- 1 until day 32. Plasma MT levels were significantly elevated (2-way ANOVA, P < 0.01) in dusky grouper juveniles in response to the MT treatment, both 6 and 12 weeks after beginning the treatment. On the contrary, no significant differences (2-way ANOVA, P > 0.05) between MT/hCG-treated and control fish were observed in plasma testosterone (T) or 11-keto T (11-KT). Pituitary luteinizing hormone (LH) content was significantly lower in both the MT/hCG and hCG groups, compared to controls (2-way ANOVA, P < 0.001), while plasma LH was below detection limits in all treatments. At the onset of the experiment all but one fish were already differentiated into females, having ovaries with primary oocytes. No signs of vitellogenesis or sex inversion were observed in fish from the control and hCG groups during the study. On the contrary, the MT implants induced complete sex change after 12 weeks, with the gonads organized in lobules and cysts filled with germ cells at all stages of spermatogenesis, including spermatozoa. This sex inversion was permanent and fish underwent complete spermatogenesis again during the following year. The results demonstrate that the produced MT-implants, but not the hCG injections, were highly efficient in inducing both sex inversion and complete spermatogenesis in pre-pubertal dusky grouper at the completion of their second year in life. Production of male fish at such an early age may dramatically reduce by many years the time required for natural puberty, sex inversion and the production of sperm by dusky grouper reared under culture conditions. © 2006 Elsevier B.V. All rights reserved. |
Koutandos, E; Prinos, P; Koutitas, C Permeability effects on breaking waves over submerged rubble mound breakwaters Inproceedings pp. 211–212, 2006, (Publication Title: International Conference ‘Hydroscience-2006’). @inproceedings{koutandos_permeability_2006, title = {Permeability effects on breaking waves over submerged rubble mound breakwaters}, author = {E Koutandos and P Prinos and C Koutitas}, year = {2006}, date = {2006-01-01}, volume = {1}, pages = {211--212}, note = {Publication Title: International Conference ‘Hydroscience-2006’}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Teuten, E L; Johnson, C G; Mandalakis, M; Asplund, L; Gustafsson, O; Unger, M; Marsh, G; Reddy, C M Spectral characterization of two bioaccumulated methoxylated polybrominated diphenyl ethers Journal Article Chemosphere, 62 (2), pp. 197–203, 2006, ISSN: 00456535. @article{teuten_spectral_2006, title = {Spectral characterization of two bioaccumulated methoxylated polybrominated diphenyl ethers}, author = {E L Teuten and C G Johnson and M Mandalakis and L Asplund and O Gustafsson and M Unger and G Marsh and C M Reddy}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-28444446663&doi=10.1016%2fj.chemosphere.2005.05.023&partnerID=40&md5=19cfac57ec819f2b14073b66d0a47aa0}, doi = {10.1016/j.chemosphere.2005.05.023}, issn = {00456535}, year = {2006}, date = {2006-01-01}, journal = {Chemosphere}, volume = {62}, number = {2}, pages = {197--203}, abstract = {Two methoxylated polybrominated diphenyl ethers (MeO-PBDEs) were isolated from a True's beaked whale (Mesoplodon mirus) and identified by NMR ( 1H, 1H-1H and 1H-13C) and high resolution mass spectrometry as 2-(2′,4′-dibromophenoxy)-3,5- dibromoanisole (6-MeO-BDE47) and 2-(2′,4′-dibromophenoxy)-4,6- dibromoanisole (2′-MeO-BDE68). Previously the structures of these bioaccumulated compounds have been determined by comparison of their mass spectra and gas chromatographic (GC) retention times with those of authentic standards. While this method is accepted and generally successful, NMR of the isolated compounds allows us to definitively identify the congeners. Our characterizations are consistent with those made for MeO-PBDEs in other organisms, identified by chromatographic methods. © 2005 Elsevier Ltd. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Two methoxylated polybrominated diphenyl ethers (MeO-PBDEs) were isolated from a True's beaked whale (Mesoplodon mirus) and identified by NMR ( 1H, 1H-1H and 1H-13C) and high resolution mass spectrometry as 2-(2′,4′-dibromophenoxy)-3,5- dibromoanisole (6-MeO-BDE47) and 2-(2′,4′-dibromophenoxy)-4,6- dibromoanisole (2′-MeO-BDE68). Previously the structures of these bioaccumulated compounds have been determined by comparison of their mass spectra and gas chromatographic (GC) retention times with those of authentic standards. While this method is accepted and generally successful, NMR of the isolated compounds allows us to definitively identify the congeners. Our characterizations are consistent with those made for MeO-PBDEs in other organisms, identified by chromatographic methods. © 2005 Elsevier Ltd. All rights reserved. |
Daffonchio, D; Borin, S; Brusa, T; Brusetti, L; Wielen, Van Der P W J J; Bolhuis, H; Yakimov, M M; D’Auria, G; Giuliano, L; Marty, D; Tamburini, C; McGenity, T J; Hallsworth, J E; Sass, A M; Timmis, K N; Telepidis, A; de Lange, G J; Hübner, A; Thomson, J; Varnaavs, S P; Gasparoni, F; Gerber, H W; Malinverno, H W; Corselli, C; Garcin, J; McKew, B; Golyshin, P N; Lampadariou, N; Polymenakou, P N; Calore, D; Cenedesse, S; Zanon, F; Hoog, S Stratified prokaryote network in the oxic-anoxic transition of a deep-sea halocline Journal Article Nature, 440 (7081), pp. 203–207, 2006. @article{daffonchio_stratified_2006, title = {Stratified prokaryote network in the oxic-anoxic transition of a deep-sea halocline}, author = {D Daffonchio and S Borin and T Brusa and L Brusetti and P W J J Van Der Wielen and H Bolhuis and M M Yakimov and G D’Auria and L Giuliano and D Marty and C Tamburini and T J McGenity and J E Hallsworth and A M Sass and K N Timmis and A Telepidis and G J de Lange and A Hübner and J Thomson and S P Varnaavs and F Gasparoni and H W Gerber and H W Malinverno and C Corselli and J Garcin and B McKew and P N Golyshin and N Lampadariou and P N Polymenakou and D Calore and S Cenedesse and F Zanon and S Hoog}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33644865958&partnerID=40&md5=45aab482809ee378554f4890a3772cb9}, doi = {https://doi.org/10.1038/nature04418}, year = {2006}, date = {2006-01-01}, journal = {Nature}, volume = {440}, number = {7081}, pages = {203--207}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Thessalou-Legaki, M; Zenetos, A; Kambouroglou, V; Corsini-Foka, M; Kouraklis, P; Dounas, C; Nicolaidou, A The establishment of the invasive crab Percnon gibbesi (Ħ. Milne Edwards, 1853) (Crustacea: Decapoda: Grapsidae) in Greek waters Journal Article Aquatic Invasions, 1 (3), pp. 133–136, 2006, ISSN: 17986540, (Publisher: Regional Euro-Asian Biological Invasions Centre). @article{thessalou-legaki_establishment_2006, title = {The establishment of the invasive crab Percnon gibbesi (Ħ. Milne Edwards, 1853) (Crustacea: Decapoda: Grapsidae) in Greek waters}, author = {M Thessalou-Legaki and A Zenetos and V Kambouroglou and M Corsini-Foka and P Kouraklis and C Dounas and A Nicolaidou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-40449137471&doi=10.3391%2fai.2006.1.3.6&partnerID=40&md5=e33e3fa817848ac61925caf3502b2991}, doi = {10.3391/ai.2006.1.3.6}, issn = {17986540}, year = {2006}, date = {2006-01-01}, journal = {Aquatic Invasions}, volume = {1}, number = {3}, pages = {133--136}, abstract = {The tropical Atlantic grapsid crab Percnon gibbesi (H. Milne Edwards, 1853) may be regarded as the most invasive decapod currently expanding its distribution in the Mediterranean Sea. The present paper records its presence in Greek waters since 2004. The species seems well established along the coasts of Messiniakos Gulf, Crete and Rhodes Islands as several individuals of various sizes were observed on shallow, rocky bottoms. The proximity of these localities to ports may hint at shipping as potential vector, but larval transport from already established populations across the Ionian Sea cannot be excluded. The wide adaptability, the potential for colonising new habitats and the population dynamics of P. gibbesi merit further investigation. © 2006 The Author(s).}, note = {Publisher: Regional Euro-Asian Biological Invasions Centre}, keywords = {}, pubstate = {published}, tppubtype = {article} } The tropical Atlantic grapsid crab Percnon gibbesi (H. Milne Edwards, 1853) may be regarded as the most invasive decapod currently expanding its distribution in the Mediterranean Sea. The present paper records its presence in Greek waters since 2004. The species seems well established along the coasts of Messiniakos Gulf, Crete and Rhodes Islands as several individuals of various sizes were observed on shallow, rocky bottoms. The proximity of these localities to ports may hint at shipping as potential vector, but larval transport from already established populations across the Ionian Sea cannot be excluded. The wide adaptability, the potential for colonising new habitats and the population dynamics of P. gibbesi merit further investigation. © 2006 The Author(s). |
Smith, C J; Papadopoulou, K -N; Dafnomili, E; Zivanovic, S The Impact of Trawling on Nutrient and Oxygen Fluxes in the Cretan Sea Inproceedings Thessaloniki, Greece, 2006, (Publication Title: 8th Hellenic Symposium on Oceanography and Fisheries). @inproceedings{smith_impact_2006, title = {The Impact of Trawling on Nutrient and Oxygen Fluxes in the Cretan Sea}, author = {C J Smith and K -N Papadopoulou and E Dafnomili and S Zivanovic}, year = {2006}, date = {2006-01-01}, address = {Thessaloniki, Greece}, note = {Publication Title: 8th Hellenic Symposium on Oceanography and Fisheries}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Sarropoulou, E; Power, D M; Mamuris, Z; Moutou, K A The two isoforms of myosin light chain 2 in gilthead sea bream (Sparus aurata); alternative polyadenylation site selection and tissue expression Journal Article Archiv fur Tierzucht, 49 , pp. 92 – 96, 2006. @article{sarropoulou_two_2006, title = {The two isoforms of myosin light chain 2 in gilthead sea bream (Sparus aurata); alternative polyadenylation site selection and tissue expression}, author = {E Sarropoulou and D M Power and Z Mamuris and K A Moutou}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33646234711&partnerID=40&md5=23611f5ac723b7135b9293effa3d7b06}, year = {2006}, date = {2006-01-01}, journal = {Archiv fur Tierzucht}, volume = {49}, pages = {92 -- 96}, abstract = {Myosin light chain 2 (MLC2) is an essential component of the myosin molecule, with a regulatory role in binding Ca2+. In gilthead sea bream, a MLC2 clone has been isolated and characterized, that encodes for a 170 aa peptide and contains three potential polyadenylation signals in the 3' UTR. In this study, the isolation of three alternative transcripts of the already known MLC2 (isoform A) is reported, along with the isolation and characterization of a second MLC2 isoform (B). All three isoform A transcripts encode the same peptide but differ in the length of their 3' UTRs (284bp, 788bp and 876bp respectively) and are generated by alternative polyadenylation site selection. Transcripts of isoform A were detected both in white and red muscle. MLC2 isoform B encodes also for a 170 aa protein. Isoform B was detected in all tissues examined (red, white, smooth and cardiac muscle, kidney, liver, spleen, brain, gills, epidermis). The differential expression of the two isoforms and of the alternative transcripts of isoform A during development is currently under study, in order to investigate the functional significance and regulation of 3' UTR length in transcription and mRNA turnover rate.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Myosin light chain 2 (MLC2) is an essential component of the myosin molecule, with a regulatory role in binding Ca2+. In gilthead sea bream, a MLC2 clone has been isolated and characterized, that encodes for a 170 aa peptide and contains three potential polyadenylation signals in the 3' UTR. In this study, the isolation of three alternative transcripts of the already known MLC2 (isoform A) is reported, along with the isolation and characterization of a second MLC2 isoform (B). All three isoform A transcripts encode the same peptide but differ in the length of their 3' UTRs (284bp, 788bp and 876bp respectively) and are generated by alternative polyadenylation site selection. Transcripts of isoform A were detected both in white and red muscle. MLC2 isoform B encodes also for a 170 aa protein. Isoform B was detected in all tissues examined (red, white, smooth and cardiac muscle, kidney, liver, spleen, brain, gills, epidermis). The differential expression of the two isoforms and of the alternative transcripts of isoform A during development is currently under study, in order to investigate the functional significance and regulation of 3' UTR length in transcription and mRNA turnover rate. |
Katharios, P; Papandroulakis, N; Divanach, P Treatment of Microcotyle sp. (Monogenea) on the gills of cage-cultured red porgy, Pagrus pagrus following baths with formalin and mebendazole Journal Article Aquaculture, 251 (2-4), pp. 167–171, 2006, ISSN: 00448486. @article{katharios_treatment_2006, title = {Treatment of Microcotyle sp. (Monogenea) on the gills of cage-cultured red porgy, Pagrus pagrus following baths with formalin and mebendazole}, author = {P Katharios and N Papandroulakis and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-31344445980&doi=10.1016%2fj.aquaculture.2005.05.046&partnerID=40&md5=91ab895aa4134d0252eb1817aaaa56a2}, doi = {10.1016/j.aquaculture.2005.05.046}, issn = {00448486}, year = {2006}, date = {2006-01-01}, journal = {Aquaculture}, volume = {251}, number = {2-4}, pages = {167--171}, abstract = {Two bath treatment trials for the control of the monogenean Microcotyle sp. in the gills of cultured red porgy, Pagrus pagrus using formalin (200 ppm for 1 h) and mebendazole (400 ppm for 1 h) were conducted. Formalin was very effective removing all the parasites from the gills of the infected fish while mebendazole produced no significant result. Based on the results of the trial, formalin was selected for the treatment of a large population of heavily infected red porgies held in sea cages. The results of the treatment are discussed. © 2005 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Two bath treatment trials for the control of the monogenean Microcotyle sp. in the gills of cultured red porgy, Pagrus pagrus using formalin (200 ppm for 1 h) and mebendazole (400 ppm for 1 h) were conducted. Formalin was very effective removing all the parasites from the gills of the infected fish while mebendazole produced no significant result. Based on the results of the trial, formalin was selected for the treatment of a large population of heavily infected red porgies held in sea cages. The results of the treatment are discussed. © 2005 Elsevier B.V. All rights reserved. |
2005 |
Polymenakou, Paraskevi N; Pusceddu, Antonio; Tselepides, Anastasios; Polychronaki, Thalia; Giannakourou, Antonia; Fiordelmondo, Carla; Hatziyanni, Eleni; Danovaro, Roberto Benthic microbial abundance and activities in an intensively trawled ecosystem (Thermaikos Gulf, Aegean Sea) Journal Article Continental Shelf Research, 25 (19-20), pp. 2570–2584, 2005, ISSN: 02784343. @article{polymenakou_benthic_2005, title = {Benthic microbial abundance and activities in an intensively trawled ecosystem (Thermaikos Gulf, Aegean Sea)}, author = {Paraskevi N Polymenakou and Antonio Pusceddu and Anastasios Tselepides and Thalia Polychronaki and Antonia Giannakourou and Carla Fiordelmondo and Eleni Hatziyanni and Roberto Danovaro}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0278434305001470}, doi = {10.1016/j.csr.2005.08.018}, issn = {02784343}, year = {2005}, date = {2005-12-01}, urldate = {2020-08-10}, journal = {Continental Shelf Research}, volume = {25}, number = {19-20}, pages = {2570--2584}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Pusceddu, A; Fiordelmondo, C; Polymenakou, P; Polychronaki, T; Tselepides, A; Danovaro, R Continental Shelf Research, 25 (19-20), pp. 2491–2505, 2005, ISSN: 02784343. @article{pusceddu_effects_2005, title = {Effects of bottom trawling on the quantity and biochemical composition of organic matter in coastal marine sediments (Thermaikos Gulf, northwestern Aegean Sea)}, author = {A Pusceddu and C Fiordelmondo and P Polymenakou and T Polychronaki and A Tselepides and R Danovaro}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0278434305001421}, doi = {10.1016/j.csr.2005.08.013}, issn = {02784343}, year = {2005}, date = {2005-12-01}, urldate = {2020-08-10}, journal = {Continental Shelf Research}, volume = {25}, number = {19-20}, pages = {2491--2505}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Cotou, E; Gremare, A; Charles, F; Hatzianestis, I; Sklivagou, E Potential toxicity of resuspended particulate matter and sediments: Environmental samples from the Bay of Banyuls-sur-Mer and Thermaikos Gulf Journal Article Continental Shelf Research, 25 (19-20), pp. 2521–2532, 2005, ISSN: 02784343. @article{cotou_potential_2005, title = {Potential toxicity of resuspended particulate matter and sediments: Environmental samples from the Bay of Banyuls-sur-Mer and Thermaikos Gulf}, author = {E Cotou and A Gremare and F Charles and I Hatzianestis and E Sklivagou}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0278434305001445}, doi = {10.1016/j.csr.2005.08.005}, issn = {02784343}, year = {2005}, date = {2005-12-01}, urldate = {2020-08-18}, journal = {Continental Shelf Research}, volume = {25}, number = {19-20}, pages = {2521--2532}, abstract = {This contribution attempts to determine whether, and to what degree, environmental samples of resuspended particulate matter and sediments exert a toxicological impact. Further, an attempt is made also to screen the toxic level of potentially hazardous sites, based upon established sediment toxicity criteria. Therefore, a rapid, cost-effective and highly sensitive biotest (bioluminescence assay, based upon marine bacteria) has been applied on: biological fluid extracts; bottom sediments; and sediment trap samples. Samples were taken either from the Bay of Banyuls-sur-Mer (northwestern Mediterranean, France) or Thermaikos Gulf (northeastern Mediterranean, Greece). Biological fluid extracts and sediment trap samples corresponded to periods of resuspension events, or preceding and following such events. The results have revealed that the sampling strategy and biotest implemented in this study might be a useful tool for screening the toxicity of resuspended matter and sediments. Resuspension events appear to be able to exert an influence on the chemical forms of the micro-pollutants; thus on their bioavailability and toxicity. Nevertheless, based upon chemical analysis combined with the bioassay, the toxic level of the sediment samples could be determined, with the level at potentially hazardous sites being characterised. © 2005 Elsevier Ltd. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This contribution attempts to determine whether, and to what degree, environmental samples of resuspended particulate matter and sediments exert a toxicological impact. Further, an attempt is made also to screen the toxic level of potentially hazardous sites, based upon established sediment toxicity criteria. Therefore, a rapid, cost-effective and highly sensitive biotest (bioluminescence assay, based upon marine bacteria) has been applied on: biological fluid extracts; bottom sediments; and sediment trap samples. Samples were taken either from the Bay of Banyuls-sur-Mer (northwestern Mediterranean, France) or Thermaikos Gulf (northeastern Mediterranean, Greece). Biological fluid extracts and sediment trap samples corresponded to periods of resuspension events, or preceding and following such events. The results have revealed that the sampling strategy and biotest implemented in this study might be a useful tool for screening the toxicity of resuspended matter and sediments. Resuspension events appear to be able to exert an influence on the chemical forms of the micro-pollutants; thus on their bioavailability and toxicity. Nevertheless, based upon chemical analysis combined with the bioassay, the toxic level of the sediment samples could be determined, with the level at potentially hazardous sites being characterised. © 2005 Elsevier Ltd. All rights reserved. |
Sfakianakis, D G; Doxa, C K; Kouttouki, S; Koumoundouros, G; Maingot, E; Divanach, P; Kentouri, M Osteological development of the vertebral column and of the fins in Diplodus puntazzo (Cetti, 1777) Journal Article Aquaculture, 250 (1-2), pp. 36–46, 2005, ISSN: 00448486. @article{sfakianakis_osteological_2005, title = {Osteological development of the vertebral column and of the fins in Diplodus puntazzo (Cetti, 1777)}, author = {D G Sfakianakis and C K Doxa and S Kouttouki and G Koumoundouros and E Maingot and P Divanach and M Kentouri}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848605002498}, doi = {10.1016/j.aquaculture.2005.03.042}, issn = {00448486}, year = {2005}, date = {2005-11-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {250}, number = {1-2}, pages = {36--46}, abstract = {The normal development of the vertebral column and of the fins in Diplodus puntazzo (Cetti, 1777) was studied. The ontogenetic pattern of the skeleton follows that described in all other Sparidae studied so far: the onset of the ontogeny of the vertebral column is at 4.9 mm total length (TL) with the formation of the first neural processes, while the vertebral centra begin forming at 5.8 mm TL and are completed by 8.1 mm TL. Pectoral supports (cleithrum, coracoid-scapula cartilage, fin plate) are the first fin elements to be formed at 2.9 mm TL and are followed by those of the caudal (4.5 mm TL), dorsal, anal (6.4 mm TL) and finally by those of the pelvic fin (8.1 mm TL). The caudal fin is the first to develop fin rays and attain the full count of lepidotrichia (5.8-7.1 mm TL), but the last to attain the full count of all its rays (dermatotrichia, 14.1 mm TL). The next fins to complete their rays are the anal (8.5 mm TL), the dorsal (10.1 mm TL) and finally the pectoral and the pelvic (10.6 mm TL). Fin meristic characters were compared between the reared and 27 wild D. puntazzo juveniles. The results of the skeletal ontogeny are compared with the pattern of development of the Sparidae. © 2005 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The normal development of the vertebral column and of the fins in Diplodus puntazzo (Cetti, 1777) was studied. The ontogenetic pattern of the skeleton follows that described in all other Sparidae studied so far: the onset of the ontogeny of the vertebral column is at 4.9 mm total length (TL) with the formation of the first neural processes, while the vertebral centra begin forming at 5.8 mm TL and are completed by 8.1 mm TL. Pectoral supports (cleithrum, coracoid-scapula cartilage, fin plate) are the first fin elements to be formed at 2.9 mm TL and are followed by those of the caudal (4.5 mm TL), dorsal, anal (6.4 mm TL) and finally by those of the pelvic fin (8.1 mm TL). The caudal fin is the first to develop fin rays and attain the full count of lepidotrichia (5.8-7.1 mm TL), but the last to attain the full count of all its rays (dermatotrichia, 14.1 mm TL). The next fins to complete their rays are the anal (8.5 mm TL), the dorsal (10.1 mm TL) and finally the pectoral and the pelvic (10.6 mm TL). Fin meristic characters were compared between the reared and 27 wild D. puntazzo juveniles. The results of the skeletal ontogeny are compared with the pattern of development of the Sparidae. © 2005 Elsevier B.V. All rights reserved. |
Polymenakou, Paraskevi N; Bertilsson, Stefan; Tselepides, Anastasios; Stephanou, Euripides G Microbial Ecology, 50 (3), pp. 447–462, 2005, ISSN: 0095-3628, 1432-184X. @article{polymenakou_bacterial_2005, title = {Bacterial Community Composition in Different Sediments from the Eastern Mediterranean Sea: a Comparison of Four 16S Ribosomal DNA Clone Libraries}, author = {Paraskevi N Polymenakou and Stefan Bertilsson and Anastasios Tselepides and Euripides G Stephanou}, url = {http://link.springer.com/10.1007/s00248-005-0005-6}, doi = {10.1007/s00248-005-0005-6}, issn = {0095-3628, 1432-184X}, year = {2005}, date = {2005-10-01}, urldate = {2020-08-10}, journal = {Microbial Ecology}, volume = {50}, number = {3}, pages = {447--462}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Polymenakou, Paraskevi N; Stephanou, Euripides G Effect of temperature and additional carbon sources on phenol degradation by an indigenous soil Pseudomonad Journal Article Biodegradation, 16 (5), pp. 403–413, 2005, ISSN: 0923-9820, 1572-9729. @article{polymenakou_effect_2005, title = {Effect of temperature and additional carbon sources on phenol degradation by an indigenous soil Pseudomonad}, author = {Paraskevi N Polymenakou and Euripides G Stephanou}, url = {http://link.springer.com/10.1007/s10532-004-3333-1}, doi = {10.1007/s10532-004-3333-1}, issn = {0923-9820, 1572-9729}, year = {2005}, date = {2005-10-01}, urldate = {2020-08-10}, journal = {Biodegradation}, volume = {16}, number = {5}, pages = {403--413}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Grigorakis, K; Alexis, M N Aquaculture Nutrition, 11 (5), pp. 341–344, 2005, ISSN: 1353-5773, 1365-2095. @article{grigorakis_effects_2005, title = {Effects of fasting on the meat quality and fat deposition of commercial-size farmed gilthead sea bream (Sparus aurata, L.) fed different dietary regimes}, author = {K Grigorakis and M N Alexis}, url = {http://doi.wiley.com/10.1111/j.1365-2095.2005.00351.x}, doi = {10.1111/j.1365-2095.2005.00351.x}, issn = {1353-5773, 1365-2095}, year = {2005}, date = {2005-10-01}, urldate = {2020-08-10}, journal = {Aquaculture Nutrition}, volume = {11}, number = {5}, pages = {341--344}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Fountoulaki, Eleni; Alexis, Maria N; Nengas, Ioannis; Venou, Barbara Effect of diet composition on nutrient digestibility and digestive enzyme levels of gilthead sea bream (Sparus aurata L.) Journal Article Aquaculture Research, 36 (13), pp. 1243–1251, 2005, ISSN: 1355-557X, 1365-2109. @article{fountoulaki_effect_2005, title = {Effect of diet composition on nutrient digestibility and digestive enzyme levels of gilthead sea bream (Sparus aurata L.)}, author = {Eleni Fountoulaki and Maria N Alexis and Ioannis Nengas and Barbara Venou}, url = {http://doi.wiley.com/10.1111/j.1365-2109.2005.01232.x}, doi = {10.1111/j.1365-2109.2005.01232.x}, issn = {1355-557X, 1365-2109}, year = {2005}, date = {2005-09-01}, urldate = {2020-08-18}, journal = {Aquaculture Research}, volume = {36}, number = {13}, pages = {1243--1251}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Bargelloni, L; Alarcon, J A; Alvarez, M C; Penzo, E; Magoulas, A; Palma, J; Patarnello, T The Atlantic–Mediterranean transition: Discordant genetic patterns in two seabream species, Diplodus puntazzo (Cetti) and Diplodus sargus (L.) Journal Article Molecular Phylogenetics and Evolution, 36 (3), pp. 523–535, 2005, ISSN: 10557903. @article{bargelloni_atlanticmediterranean_2005, title = {The Atlantic–Mediterranean transition: Discordant genetic patterns in two seabream species, Diplodus puntazzo (Cetti) and Diplodus sargus (L.)}, author = {L Bargelloni and J A Alarcon and M C Alvarez and E Penzo and A Magoulas and J Palma and T Patarnello}, url = {https://linkinghub.elsevier.com/retrieve/pii/S1055790305001430}, doi = {10.1016/j.ympev.2005.04.017}, issn = {10557903}, year = {2005}, date = {2005-09-01}, urldate = {2020-08-09}, journal = {Molecular Phylogenetics and Evolution}, volume = {36}, number = {3}, pages = {523--535}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ruane, N M; Makridis, P; Balm, P H M; Dinis, M T Skin darkness is related to cortisol, but not MSH, content in post-larval Solea senegalensis Journal Article Journal of Fish Biology, 67 (2), pp. 577–581, 2005, ISSN: 0022-1112, 1095-8649. @article{ruane_skin_2005, title = {Skin darkness is related to cortisol, but not MSH, content in post-larval Solea senegalensis}, author = {N M Ruane and P Makridis and P H M Balm and M T Dinis}, url = {http://doi.wiley.com/10.1111/j.0022-1112.2005.00747.x}, doi = {10.1111/j.0022-1112.2005.00747.x}, issn = {0022-1112, 1095-8649}, year = {2005}, date = {2005-08-01}, urldate = {2020-08-18}, journal = {Journal of Fish Biology}, volume = {67}, number = {2}, pages = {577--581}, abstract = {In this study it was shown that dark coloured post-larvae of Senegalese sole Solea senegalensis, at two different ages, had elevated cortisol concentrations compared with lighter coloured individuals. As melanophore-stimulating hormone levels were not elevated in dark coloured fish, it is possible that this hormone may not be the main melanotropic hormone involved in stress-related skin darkening. © 2005 The Fisheries Society of the British Isles.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In this study it was shown that dark coloured post-larvae of Senegalese sole Solea senegalensis, at two different ages, had elevated cortisol concentrations compared with lighter coloured individuals. As melanophore-stimulating hormone levels were not elevated in dark coloured fish, it is possible that this hormone may not be the main melanotropic hormone involved in stress-related skin darkening. © 2005 The Fisheries Society of the British Isles. |
Darby, A C; Lagnel, J; Matthew, C Z; Bourtzis, K; Maudlin, I; Welburn, S C Extrachromosomal DNA of the Symbiont Sodalis glossinidius Journal Article Journal of Bacteriology, 187 (14), pp. 5003–5007, 2005, ISSN: 0021-9193, 1098-5530. @article{darby_extrachromosomal_2005, title = {Extrachromosomal DNA of the Symbiont Sodalis glossinidius}, author = {A C Darby and J Lagnel and C Z Matthew and K Bourtzis and I Maudlin and S C Welburn}, url = {https://JB.asm.org/content/187/14/5003}, doi = {10.1128/JB.187.14.5003-5007.2005}, issn = {0021-9193, 1098-5530}, year = {2005}, date = {2005-07-01}, urldate = {2020-08-18}, journal = {Journal of Bacteriology}, volume = {187}, number = {14}, pages = {5003--5007}, abstract = {The extrachromosomal DNA of Sodalis glossinidius from two tsetse fly species was sequenced and contained four circular elements: three plasmids, pSG1 (82 kb), pSG2 (27 kb), and pSG4 (11 kb), and a bacteriophage-like pSG3 (19 kb) element. The information suggests S. glossinidius is evolving towards an obligate association with tsetse flies. Copyright © 2005, American Society for Microbiology. All Rights Reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The extrachromosomal DNA of Sodalis glossinidius from two tsetse fly species was sequenced and contained four circular elements: three plasmids, pSG1 (82 kb), pSG2 (27 kb), and pSG4 (11 kb), and a bacteriophage-like pSG3 (19 kb) element. The information suggests S. glossinidius is evolving towards an obligate association with tsetse flies. Copyright © 2005, American Society for Microbiology. All Rights Reserved. |
Cárdenas, Leyla; Hernández, Cristián E; Poulin, Elie; Magoulas, Antonios; Kornfield, Irv; Ojeda, Patricio F Origin, diversification, and historical biogeography of the genus Trachurus (Perciformes: Carangidae) Journal Article Molecular Phylogenetics and Evolution, 35 (2), pp. 496–507, 2005, ISSN: 10557903. @article{cardenas_origin_2005, title = {Origin, diversification, and historical biogeography of the genus Trachurus (Perciformes: Carangidae)}, author = {Leyla Cárdenas and Cristián E Hernández and Elie Poulin and Antonios Magoulas and Irv Kornfield and Patricio F Ojeda}, url = {https://linkinghub.elsevier.com/retrieve/pii/S1055790305000291}, doi = {10.1016/j.ympev.2005.01.011}, issn = {10557903}, year = {2005}, date = {2005-05-01}, urldate = {2020-08-09}, journal = {Molecular Phylogenetics and Evolution}, volume = {35}, number = {2}, pages = {496--507}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Makridis, P; Martins, S; Vercauteren, T; Driessche, Van K; Decamp, O; Dinis, M T Evaluation of candidate probiotic strains for gilthead sea bream larvae (Sparus aurata) using an in vivo approach Journal Article Letters in Applied Microbiology, 40 (4), pp. 274–277, 2005, ISSN: 0266-8254, 1365-2673. @article{makridis_evaluation_2005, title = {Evaluation of candidate probiotic strains for gilthead sea bream larvae (Sparus aurata) using an in vivo approach}, author = {P Makridis and S Martins and T Vercauteren and K Van Driessche and O Decamp and M T Dinis}, url = {http://doi.wiley.com/10.1111/j.1472-765X.2005.01676.x}, doi = {10.1111/j.1472-765X.2005.01676.x}, issn = {0266-8254, 1365-2673}, year = {2005}, date = {2005-04-01}, urldate = {2020-08-18}, journal = {Letters in Applied Microbiology}, volume = {40}, number = {4}, pages = {274--277}, abstract = {AIMS: The aim of this study was to evaluate the effect of six bacterial strains on gilthead sea bream larvae (Sparus aurata). METHODS AND RESULTS: Six bacterial strains isolated from well-performing live food cultures were identified by sequencing fragments of their 16s rDNA genome to the genus level as Cytophaga sp., Roseobacter sp., Ruergeria sp., Paracoccus sp., Aeromonas sp. and Shewanella sp. Survival rates of gilthead sea bream larvae transferred to seawater added these bacterial strains at concentrations of 6 +/- 0.3 x 10(5) bacteria ml(-1) were similar to those of larvae transferred to sterilized seawater and showed an average of 86% at 9 days after hatching, whereas, survival rates of larvae transferred to filtered seawater were lower (P textless 0.05), and showed an average of 39%, 9 days after hatching. CONCLUSION: Several bacterial strains isolated from well-performing live food cultures showed a positive effect for sea bream larvae when compared with filtered seawater. SIGNIFICANCE AND IMPACT OF THE STUDY: The approach used in this study could be applied as an in vivo evaluation method of candidate probiotic strains used in the rearing of marine fish larvae.}, keywords = {}, pubstate = {published}, tppubtype = {article} } AIMS: The aim of this study was to evaluate the effect of six bacterial strains on gilthead sea bream larvae (Sparus aurata). METHODS AND RESULTS: Six bacterial strains isolated from well-performing live food cultures were identified by sequencing fragments of their 16s rDNA genome to the genus level as Cytophaga sp., Roseobacter sp., Ruergeria sp., Paracoccus sp., Aeromonas sp. and Shewanella sp. Survival rates of gilthead sea bream larvae transferred to seawater added these bacterial strains at concentrations of 6 +/- 0.3 x 10(5) bacteria ml(-1) were similar to those of larvae transferred to sterilized seawater and showed an average of 86% at 9 days after hatching, whereas, survival rates of larvae transferred to filtered seawater were lower (P textless 0.05), and showed an average of 39%, 9 days after hatching. CONCLUSION: Several bacterial strains isolated from well-performing live food cultures showed a positive effect for sea bream larvae when compared with filtered seawater. SIGNIFICANCE AND IMPACT OF THE STUDY: The approach used in this study could be applied as an in vivo evaluation method of candidate probiotic strains used in the rearing of marine fish larvae. |
Polymenakou, P N; Bertilsson, S; Tselepides, A; Stephanou, E G Links between Geographic Location, Environmental Factors, and Microbial Community Composition in Sediments of the Eastern Mediterranean Sea Journal Article Microbial Ecology, 49 (3), pp. 367–378, 2005, ISSN: 0095-3628, 1432-184X. @article{polymenakou_links_2005, title = {Links between Geographic Location, Environmental Factors, and Microbial Community Composition in Sediments of the Eastern Mediterranean Sea}, author = {P N Polymenakou and S Bertilsson and A Tselepides and E G Stephanou}, url = {http://link.springer.com/10.1007/s00248-004-0274-5}, doi = {10.1007/s00248-004-0274-5}, issn = {0095-3628, 1432-184X}, year = {2005}, date = {2005-04-01}, urldate = {2020-08-10}, journal = {Microbial Ecology}, volume = {49}, number = {3}, pages = {367--378}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Duron, Olivier; Lagnel, Jacques; Raymond, Michel; Bourtzis, Kostas; Fort, Philippe; Weill, Mylene Transposable element polymorphism of Wolbachia in the mosquito Culex pipiens: evidence of genetic diversity, superinfection and recombination Journal Article Molecular Ecology, 14 (5), pp. 1561–1573, 2005, ISSN: 0962-1083, 1365-294X. @article{duron_transposable_2005, title = {Transposable element polymorphism of Wolbachia in the mosquito Culex pipiens: evidence of genetic diversity, superinfection and recombination}, author = {Olivier Duron and Jacques Lagnel and Michel Raymond and Kostas Bourtzis and Philippe Fort and Mylene Weill}, url = {http://doi.wiley.com/10.1111/j.1365-294X.2005.02495.x}, doi = {10.1111/j.1365-294X.2005.02495.x}, issn = {0962-1083, 1365-294X}, year = {2005}, date = {2005-04-01}, urldate = {2020-08-18}, journal = {Molecular Ecology}, volume = {14}, number = {5}, pages = {1561--1573}, abstract = {Wolbachia is a group of maternally inherited endosymbiotic bacteria that infect and induce cytoplasmic incompatibility (CI) in a wide range of arthropods. In contrast to other species, the mosquito Culex pipiens displays an extremely high number of CI types suggesting differential infection by multiple Wolbachia strains. Attempts so far failed to detect Wolbachia polymorphism that might explain this high level of CI diversity found in C. pipiens populations. Here, we establish that Wolbachia infection is near to or at fixation in worldwide populations of the C. pipiens complex. Wolbachia polymorphism was addressed by sequence analysis of the Tr1 gene, a unique transposable element of the IS5 family, which allowed the identification of five C. pipiens Wolbachia strains, differing either by nucleotide substitution, presence or absence pattern, or insertion site. Sequence analysis also showed that recombination, transposition and superinfection occurred at very low frequencies. Analysis of the geographical distributions of each Wolbachia strain among C. pipiens populations indicated a strong worldwide differentiation independent from mosquito subspecies type, except in the UK. The availability of this polymorphic marker now opens the way to investigate evolution of Wolbachia populations and CI dynamics, in particular in regions where multiple crossing types coexist among C. pipiens populations. © 2005 Blackwell Publishing Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Wolbachia is a group of maternally inherited endosymbiotic bacteria that infect and induce cytoplasmic incompatibility (CI) in a wide range of arthropods. In contrast to other species, the mosquito Culex pipiens displays an extremely high number of CI types suggesting differential infection by multiple Wolbachia strains. Attempts so far failed to detect Wolbachia polymorphism that might explain this high level of CI diversity found in C. pipiens populations. Here, we establish that Wolbachia infection is near to or at fixation in worldwide populations of the C. pipiens complex. Wolbachia polymorphism was addressed by sequence analysis of the Tr1 gene, a unique transposable element of the IS5 family, which allowed the identification of five C. pipiens Wolbachia strains, differing either by nucleotide substitution, presence or absence pattern, or insertion site. Sequence analysis also showed that recombination, transposition and superinfection occurred at very low frequencies. Analysis of the geographical distributions of each Wolbachia strain among C. pipiens populations indicated a strong worldwide differentiation independent from mosquito subspecies type, except in the UK. The availability of this polymorphic marker now opens the way to investigate evolution of Wolbachia populations and CI dynamics, in particular in regions where multiple crossing types coexist among C. pipiens populations. © 2005 Blackwell Publishing Ltd. |
Chistiakov, D A; Hellemans, B; Haley, C S; Law, A S; Tsigenopoulos, C S; Kotoulas, G; Bertotto, D; Libertini, A; Volckaert, F A M A microsatellite linkage map of the European sea bass Dicentrarchus labrax L. Journal Article Genetics, 170 (4), pp. 1821–1826, 2005, ISSN: 00166731. @article{chistiakov_microsatellite_2005, title = {A microsatellite linkage map of the European sea bass Dicentrarchus labrax L.}, author = {D A Chistiakov and B Hellemans and C S Haley and A S Law and C S Tsigenopoulos and G Kotoulas and D Bertotto and A Libertini and F A M Volckaert}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-25444490899&doi=10.1534%2fgenetics.104.039719&partnerID=40&md5=8dfc95ab2fdb751d0c70b2f5f1342b11}, doi = {10.1534/genetics.104.039719}, issn = {00166731}, year = {2005}, date = {2005-01-01}, journal = {Genetics}, volume = {170}, number = {4}, pages = {1821--1826}, abstract = {A genetic linkage map of the European sea bass (Dicentrarchus labrax) was constructed from 174 microsatellite markers, including 145 new markers reported in this study. The mapping panel was derived from farmed sea bass from the North Adriatic Sea and consisted of a single family including both parents and 50 full-sib progeny (biparental diploids). A total of 162 microsatellites were mapped in 25 linkage groups. Eleven loci represent type I (coding) markers; 2 loci are located within the peptide Y (linkage group 1) and cytochrome P450 aromatase (linkage group 6) genes. The sex-averaged map spans 814.5 cM of the sea bass genome. The female map covers 905.9 cM, whereas the male map covers only 567.4 cM. The constructed map represents the first linkage map of European sea bass, one of the most important aquaculture species in Europe. Copyright © 2005 by the Genetics Society of America.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A genetic linkage map of the European sea bass (Dicentrarchus labrax) was constructed from 174 microsatellite markers, including 145 new markers reported in this study. The mapping panel was derived from farmed sea bass from the North Adriatic Sea and consisted of a single family including both parents and 50 full-sib progeny (biparental diploids). A total of 162 microsatellites were mapped in 25 linkage groups. Eleven loci represent type I (coding) markers; 2 loci are located within the peptide Y (linkage group 1) and cytochrome P450 aromatase (linkage group 6) genes. The sex-averaged map spans 814.5 cM of the sea bass genome. The female map covers 905.9 cM, whereas the male map covers only 567.4 cM. The constructed map represents the first linkage map of European sea bass, one of the most important aquaculture species in Europe. Copyright © 2005 by the Genetics Society of America. |
Brown, R C; Tsalavouta, M; Terzoglou, V; Magoulas, A; McAndrew, B J Additional microsatellites for Sparus aurata and cross-species amplification within the Sparidae family Journal Article Molecular Ecology Notes, 5 , pp. 605 – 607, 2005. @article{brown_additional_2005, title = {Additional microsatellites for Sparus aurata and cross-species amplification within the Sparidae family}, author = {R C Brown and M Tsalavouta and V Terzoglou and A Magoulas and B J McAndrew}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-28344455572&partnerID=40&md5=7f56611349fb1cbe98f6e3fbe1c0e1a7}, doi = {10.1111/j.1471-8286.2005.01007.x}, year = {2005}, date = {2005-01-01}, journal = {Molecular Ecology Notes}, volume = {5}, pages = {605 -- 607}, abstract = {Six new microsatellite loci were isolated and characterized in 32 individuals from a farm population of gilthead seabream (Sparus aurata). Expected heterozygosity at all loci was high, ranging from 0.835 to 0.958 with between 10 and 27 alleles per locus. A multiplex polymerase chain reaction protocol was developed using four of the loci. Cross-species amplification of the loci was tested in six species of the Sparidae family and four loci were successfully amplified in two or more related species. © 2005 Blackwell Publishing Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Six new microsatellite loci were isolated and characterized in 32 individuals from a farm population of gilthead seabream (Sparus aurata). Expected heterozygosity at all loci was high, ranging from 0.835 to 0.958 with between 10 and 27 alleles per locus. A multiplex polymerase chain reaction protocol was developed using four of the loci. Cross-species amplification of the loci was tested in six species of the Sparidae family and four loci were successfully amplified in two or more related species. © 2005 Blackwell Publishing Ltd. |
Richardson, C A; Kingsley-Smith, P R; Seed, R; Chatzinikolaou, E Marine Biology, 148 (2), pp. 319–326, 2005, ISSN: 00253162. @article{richardson_age_2005, title = {Age and growth of the naticid gastropod Polinices pulchellus (Gastropoda: Naticidae) based on length frequency analysis and statolith growth rings}, author = {C A Richardson and P R Kingsley-Smith and R Seed and E Chatzinikolaou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-28444483588&doi=10.1007%2fs00227-005-0072-8&partnerID=40&md5=037cebd2b1e1ca9d1ed2d2bbb9e5bc45}, doi = {10.1007/s00227-005-0072-8}, issn = {00253162}, year = {2005}, date = {2005-01-01}, journal = {Marine Biology}, volume = {148}, number = {2}, pages = {319--326}, abstract = {In Red Wharf Bay, UK the naticid gastropod, Polinices pulchellus, was more abundant and more highly aggregated during the summer months (June-August 2001) than during the winter (December 2000). Whilst small numbers of juvenile P. pulchellus (4-6 mm shell length) were present throughout the year the population consisted mainly of individuals of 12-14 mm shell length. Juvenile snails grew rapidly in size during the winter and early spring; growth then virtually ceased between May and June, following which there was a further period of rapid growth between August and February. Densities ranged between 57 and 4,073 ha-1 and the largest individual collected during this investigation measured 16.2 mm in shell length. Statoliths from adult P. pulchellus revealed the presence of a settlement ring and two prominent growth rings (rings 1 and 2). A curvilinear relationship exists between statolith diameter and shell length in snails up to 16 mm in length. Settlement rings ranged in diameter from 19.7 to 45.2 μm (mean 29.8 μm; SE=0.41) giving an estimated shell length of the settled juvenile of 1.1 mm. The diameter of ring 1 and ring 2 were significantly correlated indicating that rapid growth during the first year is maintained during year 2. Shell lengths estimated from the diameters of the prominent statolith rings and those obtained from length frequency data analysis (LFDA), were broadly congruent strongly suggesting an annual periodicity to the statolith rings. The largest snails (>15 mm) present within this population were estimated to be between 2 and 3 years old. Von Bertallanfy seasonal growth curves obtained from the LFDA predicted values of L∞, K and t 0 of 14.32 mm, 1.54 and -0.14 years, respectively, suggesting that P. pulchellus rapidly attains its maximum asymptotic size. © Springer-Verlag 2005.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In Red Wharf Bay, UK the naticid gastropod, Polinices pulchellus, was more abundant and more highly aggregated during the summer months (June-August 2001) than during the winter (December 2000). Whilst small numbers of juvenile P. pulchellus (4-6 mm shell length) were present throughout the year the population consisted mainly of individuals of 12-14 mm shell length. Juvenile snails grew rapidly in size during the winter and early spring; growth then virtually ceased between May and June, following which there was a further period of rapid growth between August and February. Densities ranged between 57 and 4,073 ha-1 and the largest individual collected during this investigation measured 16.2 mm in shell length. Statoliths from adult P. pulchellus revealed the presence of a settlement ring and two prominent growth rings (rings 1 and 2). A curvilinear relationship exists between statolith diameter and shell length in snails up to 16 mm in length. Settlement rings ranged in diameter from 19.7 to 45.2 μm (mean 29.8 μm; SE=0.41) giving an estimated shell length of the settled juvenile of 1.1 mm. The diameter of ring 1 and ring 2 were significantly correlated indicating that rapid growth during the first year is maintained during year 2. Shell lengths estimated from the diameters of the prominent statolith rings and those obtained from length frequency data analysis (LFDA), were broadly congruent strongly suggesting an annual periodicity to the statolith rings. The largest snails (>15 mm) present within this population were estimated to be between 2 and 3 years old. Von Bertallanfy seasonal growth curves obtained from the LFDA predicted values of L∞, K and t 0 of 14.32 mm, 1.54 and -0.14 years, respectively, suggesting that P. pulchellus rapidly attains its maximum asymptotic size. © Springer-Verlag 2005. |
Rigos, G; Troisi, G M Reviews in Fish Biology and Fisheries, 15 , pp. 53 – 73, 2005. @article{rigos_antibacterial_2005, title = {Antibacterial agents in Mediterranean finfish farming: A synopsis of drug pharmacokinetics in important euryhaline fish species and possible environmental implications}, author = {G Rigos and G M Troisi}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-29544446441&partnerID=40&md5=baa5e88d117bde3be196951065389749}, year = {2005}, date = {2005-01-01}, journal = {Reviews in Fish Biology and Fisheries}, volume = {15}, pages = {53 -- 73}, abstract = {The literature pertaining to the use of registered antibacterial agents in Mediterranean finfish farming is reviewed, with an emphasis on the Greek fish-farming industry. This review provides a scientific resource dedicated to the design of future antibacterial dosing regimes in Mediterranean fish farming, where insufficient supporting information is currently available. This paper addresses the paucity in knowledge concerning pharmacokinetics and the efficacy and environmental impact of commonly used antibacterials needed to direct future research and promote good practices in the euryhaline fish farming industry. Several registered antibacterials are currently available for combating bacterial infections, including tetracyclines, (fluoro) quinolones, potentiated sulfa, penicillin and chloramphenicol derivatives. Based on the available data, oxytetracycline (OTC) and quinolone drugs (oxolinic acid - OA and flumequine - FLU) are the most widely used in Mediterranean aquaculture. As a result these drugs have received the most extensive studies, whereas, there is considerable paucity of reliable data on pharmacokinetic and the depletion characteristics of other drugs used, particularly potentiated sulfa, penicillin derivatives and florfenicol. We find there is incomplete data on drug efficacy and minimum inhibitory concentrations (MIC) for common antibacterials used against the major bacterial pathogens of Mediterranean fish species. Furthermore, a considerable lack of data on environmental drug concentrations around Mediterranean fish farms was also identified, highlighting the need for more extensive environmental studies to monitor contamination in environmental components i.e., water and sediment, and in non-target species (flora and fauna). Prudent selection and use of antibacterials can encourage lower dosage applications, enhance treatment efficacy, and help to minimize contamination of the environment. Selection of readily bioavailable drugs which have low environmental persistence, low aquatic toxicity and high antibacterial efficacy is advised, to reduce potential losses to the environment and associated toxic effects on target species and the development of bacterial resistance. Lack of present data made it impossible to provide thorough and accurate guidance on selection and use of antibacterials and approaches for minimizing environmental impacts for the treatment of major euryhaline aquaculture species. © Springer 2005.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The literature pertaining to the use of registered antibacterial agents in Mediterranean finfish farming is reviewed, with an emphasis on the Greek fish-farming industry. This review provides a scientific resource dedicated to the design of future antibacterial dosing regimes in Mediterranean fish farming, where insufficient supporting information is currently available. This paper addresses the paucity in knowledge concerning pharmacokinetics and the efficacy and environmental impact of commonly used antibacterials needed to direct future research and promote good practices in the euryhaline fish farming industry. Several registered antibacterials are currently available for combating bacterial infections, including tetracyclines, (fluoro) quinolones, potentiated sulfa, penicillin and chloramphenicol derivatives. Based on the available data, oxytetracycline (OTC) and quinolone drugs (oxolinic acid - OA and flumequine - FLU) are the most widely used in Mediterranean aquaculture. As a result these drugs have received the most extensive studies, whereas, there is considerable paucity of reliable data on pharmacokinetic and the depletion characteristics of other drugs used, particularly potentiated sulfa, penicillin derivatives and florfenicol. We find there is incomplete data on drug efficacy and minimum inhibitory concentrations (MIC) for common antibacterials used against the major bacterial pathogens of Mediterranean fish species. Furthermore, a considerable lack of data on environmental drug concentrations around Mediterranean fish farms was also identified, highlighting the need for more extensive environmental studies to monitor contamination in environmental components i.e., water and sediment, and in non-target species (flora and fauna). Prudent selection and use of antibacterials can encourage lower dosage applications, enhance treatment efficacy, and help to minimize contamination of the environment. Selection of readily bioavailable drugs which have low environmental persistence, low aquatic toxicity and high antibacterial efficacy is advised, to reduce potential losses to the environment and associated toxic effects on target species and the development of bacterial resistance. Lack of present data made it impossible to provide thorough and accurate guidance on selection and use of antibacterials and approaches for minimizing environmental impacts for the treatment of major euryhaline aquaculture species. © Springer 2005. |
Makridis, P; Martins, S; Tsalavouta, M; Dionisio, L C; Kotoulas, G; Magoulas, A; Dinis, M T Antimicrobial activity in bacteria isolated from Senegalese sole, Solea senegalensis, fed with natural prey Journal Article Aquaculture Research, 36 (16), pp. 1619–1627, 2005, ISSN: 1355557X. @article{makridis_antimicrobial_2005, title = {Antimicrobial activity in bacteria isolated from Senegalese sole, Solea senegalensis, fed with natural prey}, author = {P Makridis and S Martins and M Tsalavouta and L C Dionisio and G Kotoulas and A Magoulas and M T Dinis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-29044440557&doi=10.1111%2fj.1365-2109.2005.01388.x&partnerID=40&md5=64efc6efd5ef382391232a269eb428d1}, doi = {10.1111/j.1365-2109.2005.01388.x}, issn = {1355557X}, year = {2005}, date = {2005-01-01}, journal = {Aquaculture Research}, volume = {36}, number = {16}, pages = {1619--1627}, abstract = {Intestinal bacteria in marine fish may produce antimicrobial substances which inhibit pathogenic bacteria. The aim of this study was to determine the influence of a change of fish diet on the antimicrobial activity of the culturable aerobic gut microflora of Senegalese sole, Solea senegalensis. Pre-adult 15-month-old fish previously fed on an artificial diet, were fed polychaetes (Hediste diversicolor), which form part of the natural diet of Senegalese sole. Samples were taken 0, 3 and 6 weeks after start of the experiment from the stomach, small and large intestine of the fish. The bacterial strains isolated from these samples were sub-cultured to pure cultures and stored at - 80°C. Several biochemical tests were run to obtain some basic phenotypic characteristics of the isolated strains. Amplification and sequencing of 16S rDNA fragments were used to identify the majority of the bacterial strains isolated. The identification by use of this molecular approach gave results in agreement to the phenotypic characterization. Feeding with polychaetes significantly increased (P textless 0.05) the numbers of presumptive Vibrio isolates in the gut. The number of bacterial strains with antimicrobial activity, as determined by two in vitro approaches, was significantly (P textless 0.05) increased by feeding with polychaetes. © 2005 Blackwell Publishing Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Intestinal bacteria in marine fish may produce antimicrobial substances which inhibit pathogenic bacteria. The aim of this study was to determine the influence of a change of fish diet on the antimicrobial activity of the culturable aerobic gut microflora of Senegalese sole, Solea senegalensis. Pre-adult 15-month-old fish previously fed on an artificial diet, were fed polychaetes (Hediste diversicolor), which form part of the natural diet of Senegalese sole. Samples were taken 0, 3 and 6 weeks after start of the experiment from the stomach, small and large intestine of the fish. The bacterial strains isolated from these samples were sub-cultured to pure cultures and stored at - 80°C. Several biochemical tests were run to obtain some basic phenotypic characteristics of the isolated strains. Amplification and sequencing of 16S rDNA fragments were used to identify the majority of the bacterial strains isolated. The identification by use of this molecular approach gave results in agreement to the phenotypic characterization. Feeding with polychaetes significantly increased (P textless 0.05) the numbers of presumptive Vibrio isolates in the gut. The number of bacterial strains with antimicrobial activity, as determined by two in vitro approaches, was significantly (P textless 0.05) increased by feeding with polychaetes. © 2005 Blackwell Publishing Ltd. |
Sarropoulou, Elena; Power, D M; A, Magoulas; Geisler, R; Kotoulas, Giorgios Comparative analysis and characterization of expressed sequence tags in gilthead sea bream (Sparus aurata) liver and embryos. Journal Article Aquaculture, 243 (1-4), pp. 69–81, 2005. @article{sarropoulou_comparative_2005, title = {Comparative analysis and characterization of expressed sequence tags in gilthead sea bream (Sparus aurata) liver and embryos.}, author = {Elena Sarropoulou and D M Power and Magoulas A and R Geisler and Giorgios Kotoulas}, year = {2005}, date = {2005-01-01}, journal = {Aquaculture}, volume = {243}, number = {1-4}, pages = {69--81}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Mylonas, C C; Cardinaletti, G; Sigelaki, I; Polzonetti-Magni, A Aquaculture, 246 (1-4), pp. 467–481, 2005, ISSN: 00448486, (Publisher: Elsevier). @article{mylonas_comparative_2005, title = {Comparative efficacy of clove oil and 2-phenoxyethanol as anesthetics in the aquaculture of European sea bass (Dicentrarchus labrax) and gilthead sea bream (Sparus aurata) at different temperatures}, author = {C C Mylonas and G Cardinaletti and I Sigelaki and A Polzonetti-Magni}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-17944372332&doi=10.1016%2fj.aquaculture.2005.02.046&partnerID=40&md5=c28d66ffa819306390f68fb47b57ced5}, doi = {10.1016/j.aquaculture.2005.02.046}, issn = {00448486}, year = {2005}, date = {2005-01-01}, journal = {Aquaculture}, volume = {246}, number = {1-4}, pages = {467--481}, abstract = {The efficacy of clove oil as an anesthetic was evaluated in juvenile European sea bass (Dicentrarchus labrax) and gilthead sea bream (Sparus aurata), and was compared to the commonly used 2-phenoxyethanol through a series of experiments simulating aquaculture activities. Firstly, using as a criterion the acquisition of complete anesthesia (stage A5) in < 3 min and recovery (stage R5) in < 10 min, the optimal doses at 25°C were determined to be 40 mg l-1 of clove oil for both species, and 350 mg l-1 and 300 mg l-1 of 2-phenoxyethanol for European sea bass and gilthead sea bream, respectively. At 15°C, the optimal doses for the European sea bass were determined to be around 30 mg l-1 clove oil and 300 mg l -1 2-phenoxyethanol, and for gilthead sea bream 55 mg l-1 clove oil and 450 mg l-1 2-phenoxyethanol. Increasing the exposure time of fish to the optimal anesthetic dose for 5, 10 or 15 min after stage A5 anesthesia prolonged recovery time (ANOVA, P < 0.001), especially in gilthead sea bream, which also suffered significant mortality (10-83%). As expected, the lower temperature resulted in significantly longer anesthesia induction and recovery times (ANOVA, P < 0.001), presumably due to the positive relationship between temperature, and opercular ventilation rates (ANOVA, P < 0.001) and metabolism. Finally, repeated exposure to anesthetics at 0 h, 3 h and 24 h increased significantly the induction time to stage A5 anesthesia (ANOVA, P < 0.001), suggesting the development of a slight tolerance, especially to the clove oil. The study demonstrated that clove oil can be used as an effective anesthetic in European sea bass and gilthead sea bream aquaculture, at almost 10-fold lower doses than 2-phenoxyethanol. The observed differences in (a) dose response, (b) anesthesia induction and recovery times, (c) ventilation rates and (d) mortality after prolonged exposure among the two species, underscore the need to undertake extensive studies with the specific fish species, anesthetic and experimental procedure employed, before clove oil or any other anesthetic is proposed for commercial use in an aquaculture species. © 2005 Elsevier B.V. All rights reserved.}, note = {Publisher: Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {article} } The efficacy of clove oil as an anesthetic was evaluated in juvenile European sea bass (Dicentrarchus labrax) and gilthead sea bream (Sparus aurata), and was compared to the commonly used 2-phenoxyethanol through a series of experiments simulating aquaculture activities. Firstly, using as a criterion the acquisition of complete anesthesia (stage A5) in < 3 min and recovery (stage R5) in < 10 min, the optimal doses at 25°C were determined to be 40 mg l-1 of clove oil for both species, and 350 mg l-1 and 300 mg l-1 of 2-phenoxyethanol for European sea bass and gilthead sea bream, respectively. At 15°C, the optimal doses for the European sea bass were determined to be around 30 mg l-1 clove oil and 300 mg l -1 2-phenoxyethanol, and for gilthead sea bream 55 mg l-1 clove oil and 450 mg l-1 2-phenoxyethanol. Increasing the exposure time of fish to the optimal anesthetic dose for 5, 10 or 15 min after stage A5 anesthesia prolonged recovery time (ANOVA, P < 0.001), especially in gilthead sea bream, which also suffered significant mortality (10-83%). As expected, the lower temperature resulted in significantly longer anesthesia induction and recovery times (ANOVA, P < 0.001), presumably due to the positive relationship between temperature, and opercular ventilation rates (ANOVA, P < 0.001) and metabolism. Finally, repeated exposure to anesthetics at 0 h, 3 h and 24 h increased significantly the induction time to stage A5 anesthesia (ANOVA, P < 0.001), suggesting the development of a slight tolerance, especially to the clove oil. The study demonstrated that clove oil can be used as an effective anesthetic in European sea bass and gilthead sea bream aquaculture, at almost 10-fold lower doses than 2-phenoxyethanol. The observed differences in (a) dose response, (b) anesthesia induction and recovery times, (c) ventilation rates and (d) mortality after prolonged exposure among the two species, underscore the need to undertake extensive studies with the specific fish species, anesthetic and experimental procedure employed, before clove oil or any other anesthetic is proposed for commercial use in an aquaculture species. © 2005 Elsevier B.V. All rights reserved. |
Kitsos, M -S; Christodoulou, M; Arvanitidis, C; Mavidis, M; Kirmitzoglou, I; Koukouras, A Composition of the organismic assemblage associated with Caretta caretta Journal Article Journal of the Marine Biological Association of the United Kingdom, 85 (2), pp. 257–261, 2005, ISSN: 00253154. @article{kitsos_composition_2005, title = {Composition of the organismic assemblage associated with Caretta caretta}, author = {M -S Kitsos and M Christodoulou and C Arvanitidis and M Mavidis and I Kirmitzoglou and A Koukouras}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-18244363640&doi=10.1017%2fS0025315405011136h&partnerID=40&md5=557179d4cedaf466179b5740074433b9}, doi = {10.1017/S0025315405011136h}, issn = {00253154}, year = {2005}, date = {2005-01-01}, journal = {Journal of the Marine Biological Association of the United Kingdom}, volume = {85}, number = {2}, pages = {257--261}, abstract = {The organismic assemblage associated with Caretta caretta was studied in 37 loggerhead turtles, washed ashore dead at various localities in the northern Aegean Sea. A total number of 63 macrobenthic species and 17 species of macroalgae were identified. From these species, 41 and 13 respectively are reported for the first time as epibionts of this turtle. Analysis of the epibiont species distribution on six loggerhead turtles gave some evidence regarding the settlement preferences of certain epibiont species. From a biogeographical point of view, 61.3% of the epibiont species were cosmopolitan, 30.7% had an Atlanta-Mediterranean distribution, while 8% were Mediterranean endemics.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The organismic assemblage associated with Caretta caretta was studied in 37 loggerhead turtles, washed ashore dead at various localities in the northern Aegean Sea. A total number of 63 macrobenthic species and 17 species of macroalgae were identified. From these species, 41 and 13 respectively are reported for the first time as epibionts of this turtle. Analysis of the epibiont species distribution on six loggerhead turtles gave some evidence regarding the settlement preferences of certain epibiont species. From a biogeographical point of view, 61.3% of the epibiont species were cosmopolitan, 30.7% had an Atlanta-Mediterranean distribution, while 8% were Mediterranean endemics. |
Mandalakis, M; Gustafsson, Ö; Alsberg, T; Egebäck, A -L; Reddy, C M; Xu, L; Klanova, J; Holoubek, I; Stephanou, E G Contribution of biomass burning to atmospheric polycyclic aromatic hydrocarbons at three european background sites Journal Article Environmental Science and Technology, 39 (9), pp. 2976–2982, 2005, ISSN: 0013936X. @article{mandalakis_contribution_2005, title = {Contribution of biomass burning to atmospheric polycyclic aromatic hydrocarbons at three european background sites}, author = {M Mandalakis and Ö Gustafsson and T Alsberg and A -L Egebäck and C M Reddy and L Xu and J Klanova and I Holoubek and E G Stephanou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-18344396135&doi=10.1021%2fes048184v&partnerID=40&md5=e821f5d70d448ac54af11b534fa61cfa}, doi = {10.1021/es048184v}, issn = {0013936X}, year = {2005}, date = {2005-01-01}, journal = {Environmental Science and Technology}, volume = {39}, number = {9}, pages = {2976--2982}, abstract = {Radiocarbon analysis of atmospheric polycyclic aromatic hydrocarbons (PAHs) from three background areas in Sweden, Croatia, and Greece was performed to apportion their origin between fossil and biomass combustion. Diagnostic ratios of PAHs implied that wood and coal combustion was relatively more important in the northern European site, while combustion of fossil fuels was the dominant source of PAHs to the two central-southern European background sites. The radiocarbon content (Δ14C) of atmospheric PAHs in Sweden ranged between -388‰ and -381‰, while more depleted values were observed for Greece (-914‰) and Croatia (-888‰). Using a 14C isotopic mass balance model it was calculated that biomass burning contributes nearly 10% of the total PAH burden in the studied southern European atmosphere with fossil fuel combustion making up the 90% balance. In contrast, biomass burning contributes about 50% of total PAHs in the atmosphere at the Swedish site. Our results suggest that the relative contributions of biomass burning and fossil fuels to atmospheric PAHs may differ considerably between countries, and therefore, different national control strategies might be needed if a further reduction of these pollutants is to be achieved on a continental-global scale. © 2005 American Chemical Society.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Radiocarbon analysis of atmospheric polycyclic aromatic hydrocarbons (PAHs) from three background areas in Sweden, Croatia, and Greece was performed to apportion their origin between fossil and biomass combustion. Diagnostic ratios of PAHs implied that wood and coal combustion was relatively more important in the northern European site, while combustion of fossil fuels was the dominant source of PAHs to the two central-southern European background sites. The radiocarbon content (Δ14C) of atmospheric PAHs in Sweden ranged between -388‰ and -381‰, while more depleted values were observed for Greece (-914‰) and Croatia (-888‰). Using a 14C isotopic mass balance model it was calculated that biomass burning contributes nearly 10% of the total PAH burden in the studied southern European atmosphere with fossil fuel combustion making up the 90% balance. In contrast, biomass burning contributes about 50% of total PAHs in the atmosphere at the Swedish site. Our results suggest that the relative contributions of biomass burning and fossil fuels to atmospheric PAHs may differ considerably between countries, and therefore, different national control strategies might be needed if a further reduction of these pollutants is to be achieved on a continental-global scale. © 2005 American Chemical Society. |
Koutandos, E; Prinos, P Design formulae for wave transmission behind floating breakwaters Inproceedings 2005, (Publication Title: XXXI IAHR Congress). @inproceedings{koutandos_design_2005, title = {Design formulae for wave transmission behind floating breakwaters}, author = {E Koutandos and P Prinos}, year = {2005}, date = {2005-01-01}, volume = {1}, note = {Publication Title: XXXI IAHR Congress}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Chatzifotis, S; Papadakis, I E; Divanach, P Effect of dietary water on growth of dentex Dentex dentex Journal Article Fisheries Science, 71 (6), pp. 1243–1248, 2005, ISSN: 09199268. @article{chatzifotis_effect_2005, title = {Effect of dietary water on growth of dentex Dentex dentex}, author = {S Chatzifotis and I E Papadakis and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-31144439969&doi=10.1111%2fj.1444-2906.2005.01089.x&partnerID=40&md5=8d72e22c36d27bfa837cdc0365529fd7}, doi = {10.1111/j.1444-2906.2005.01089.x}, issn = {09199268}, year = {2005}, date = {2005-01-01}, journal = {Fisheries Science}, volume = {71}, number = {6}, pages = {1243--1248}, abstract = {This study is concerned with the effect of hyrdated diets on growth and feed performance of Dentex dentex. Three levels of dietary water were tested: 7%, 20% and 40%. For the 7% level a commercial feed was used and the 20% and 40% water levels were obtained after adding an appropriate amount of deionized water to the commercial feed and mixing the pellets well. The diets were offered to fish by self-feeders, by which fish were allowed continuous access to diets after activating the self-feeders; by belt feeders, by which the diets were fed continuously and in excess over a 12 h interval; and by hand to apparent satiation two times a day. After 151 days of feeding, the group fed on the 20%-water diet showed significantly (P textless 0.05) higher growth rate than the 7 and 40% groups, while no significant differences were observed in feed conversion rate or daily feed consumption. The means of feed delivery did not influence growth rate. Self-feeder, belt and hand feeding all gave comparable growth. Fish fed the more condensed 7%-water diet had smaller stomachs than fish fed the larger volume 40%-water diet. In the stomach, all groups of fish hydrated their digesta within 2 h after feeding to a moisture content of around 60%. Apparently no difference in digestive process was observed between groups. It was concluded that the addition of water in diet at the level of 20% has a beneficial effect on dentex growth.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study is concerned with the effect of hyrdated diets on growth and feed performance of Dentex dentex. Three levels of dietary water were tested: 7%, 20% and 40%. For the 7% level a commercial feed was used and the 20% and 40% water levels were obtained after adding an appropriate amount of deionized water to the commercial feed and mixing the pellets well. The diets were offered to fish by self-feeders, by which fish were allowed continuous access to diets after activating the self-feeders; by belt feeders, by which the diets were fed continuously and in excess over a 12 h interval; and by hand to apparent satiation two times a day. After 151 days of feeding, the group fed on the 20%-water diet showed significantly (P textless 0.05) higher growth rate than the 7 and 40% groups, while no significant differences were observed in feed conversion rate or daily feed consumption. The means of feed delivery did not influence growth rate. Self-feeder, belt and hand feeding all gave comparable growth. Fish fed the more condensed 7%-water diet had smaller stomachs than fish fed the larger volume 40%-water diet. In the stomach, all groups of fish hydrated their digesta within 2 h after feeding to a moisture content of around 60%. Apparently no difference in digestive process was observed between groups. It was concluded that the addition of water in diet at the level of 20% has a beneficial effect on dentex growth. |
Arvanitidis, C; Chatzigeorgiou, G; Koutsoubas, D; Kevrekidis, T; Dounas, C; Eleftheriou, A; Koulouri, P; Mogias, A Estimating lagoonal biodiversity in Greece: Comparison of rapid assessment techniques Journal Article Helgoland Marine Research, 59 (3), pp. 177–186, 2005, ISSN: 1438387X. @article{arvanitidis_estimating_2005, title = {Estimating lagoonal biodiversity in Greece: Comparison of rapid assessment techniques}, author = {C Arvanitidis and G Chatzigeorgiou and D Koutsoubas and T Kevrekidis and C Dounas and A Eleftheriou and P Koulouri and A Mogias}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-24944557295&doi=10.1007%2fs10152-005-0216-8&partnerID=40&md5=b9d9a7372762c2833f2e6ea1c70d270e}, doi = {10.1007/s10152-005-0216-8}, issn = {1438387X}, year = {2005}, date = {2005-01-01}, journal = {Helgoland Marine Research}, volume = {59}, number = {3}, pages = {177--186}, abstract = {An attempt is made to compare the results of different rapid biodiversity assessment techniques at the pan-Mediterranean, sectorial and local levels. A uniform multivariate pattern exists at the pan-Mediterranean and national (sectorial) levels: lagoons can be different when they host only a few species, but as species numbers increase, lagoons become homogenous in composition. Multivariate techniques cannot distinguish anthropogenically-impacted lagoons from those, which are naturally disturbed. In the pan-Mediterranean context it is the higher taxonomic levels, but in the national and local context it is the most abundant macrobenthic groups (polychaetes, molluscs and crustaceans) and meiobenthos which provide patterns closest to that derived from the species level. Taxonomic distinctness indices applied to polychaete and mollusc inventories provide meaningful results at most levels and scales of observation. These indices seem to be robust enough to discriminate anthropogenically impacted from naturally disturbed lagoons. © Springer-Verlag and AWI 2005.}, keywords = {}, pubstate = {published}, tppubtype = {article} } An attempt is made to compare the results of different rapid biodiversity assessment techniques at the pan-Mediterranean, sectorial and local levels. A uniform multivariate pattern exists at the pan-Mediterranean and national (sectorial) levels: lagoons can be different when they host only a few species, but as species numbers increase, lagoons become homogenous in composition. Multivariate techniques cannot distinguish anthropogenically-impacted lagoons from those, which are naturally disturbed. In the pan-Mediterranean context it is the higher taxonomic levels, but in the national and local context it is the most abundant macrobenthic groups (polychaetes, molluscs and crustaceans) and meiobenthos which provide patterns closest to that derived from the species level. Taxonomic distinctness indices applied to polychaete and mollusc inventories provide meaningful results at most levels and scales of observation. These indices seem to be robust enough to discriminate anthropogenically impacted from naturally disturbed lagoons. © Springer-Verlag and AWI 2005. |
Papandroulakis, N; Mylonas, C C; Maingot, E; Divanach, P First results of greater amberjack (Seriola dumerili) larval rearing in mesocosm Journal Article Aquaculture, 250 (1-2), pp. 155–161, 2005, ISSN: 00448486. @article{papandroulakis_first_2005, title = {First results of greater amberjack (Seriola dumerili) larval rearing in mesocosm}, author = {N Papandroulakis and C C Mylonas and E Maingot and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-27344450721&doi=10.1016%2fj.aquaculture.2005.02.036&partnerID=40&md5=85aba7698333eb1cdf77b60229a40d1b}, doi = {10.1016/j.aquaculture.2005.02.036}, issn = {00448486}, year = {2005}, date = {2005-01-01}, journal = {Aquaculture}, volume = {250}, number = {1-2}, pages = {155--161}, abstract = {Species diversification is considered a major approach for the sustainable development of aquaculture. The greater amberjack (Seriola dumerili) has particular characteristics-advantages making it an appropriate candidate: high growth rate, large size, and established worldwide market. In the present study, the mesocosm method for larval rearing was applied, since it has been shown to be effective in the larval rearing of several species so far. This method is a semi-intensive technology, based on daily exogenous food addition, but also having the capacity of some endogenous productivity. Greater amberjack eggs were obtained from wild-caught fish matured in captivity in 6 years, after induced spawning with implants containing gonadotropin-releasing hormone agonist (GnRHa). A total of 9800 eggs survived after embryo appearance and were incubated in a 40-m3 tank with natural seawater of 40 psu. Rearing lasted 40 days. After mouth opening on day 2 post hatching, exogenous feeding with rotifers, Artemia nauplii and inert feed was initiated, while endogenous produced copepods contributed as food for the larvae from day 7 post hatching onwards. During rearing, larvae grew with an exponential rate of 0.073 day -1 in terms of total length (TL), and reached 39.9 ± 5.4 mm and 0.5 ± 0.1 g body weight at the end of the trial. All larvae inflated their swim bladder and completed metamorphosis at about 5 mm and 8 mm TL, respectively. Schooling behavior was first observed when larvae reached 9-10 mm TL, while aggression against the smallest individuals was first noticed the same period. The final population of about 350 individuals (3.5% survival) was transferred at the end of the trial for subsequent on-growing. The results obtained indicate the reliability of the technology for the larval rearing of the greater amberjack, and also its appropriateness for diversification with difficult marine species. © 2005 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Species diversification is considered a major approach for the sustainable development of aquaculture. The greater amberjack (Seriola dumerili) has particular characteristics-advantages making it an appropriate candidate: high growth rate, large size, and established worldwide market. In the present study, the mesocosm method for larval rearing was applied, since it has been shown to be effective in the larval rearing of several species so far. This method is a semi-intensive technology, based on daily exogenous food addition, but also having the capacity of some endogenous productivity. Greater amberjack eggs were obtained from wild-caught fish matured in captivity in 6 years, after induced spawning with implants containing gonadotropin-releasing hormone agonist (GnRHa). A total of 9800 eggs survived after embryo appearance and were incubated in a 40-m3 tank with natural seawater of 40 psu. Rearing lasted 40 days. After mouth opening on day 2 post hatching, exogenous feeding with rotifers, Artemia nauplii and inert feed was initiated, while endogenous produced copepods contributed as food for the larvae from day 7 post hatching onwards. During rearing, larvae grew with an exponential rate of 0.073 day -1 in terms of total length (TL), and reached 39.9 ± 5.4 mm and 0.5 ± 0.1 g body weight at the end of the trial. All larvae inflated their swim bladder and completed metamorphosis at about 5 mm and 8 mm TL, respectively. Schooling behavior was first observed when larvae reached 9-10 mm TL, while aggression against the smallest individuals was first noticed the same period. The final population of about 350 individuals (3.5% survival) was transferred at the end of the trial for subsequent on-growing. The results obtained indicate the reliability of the technology for the larval rearing of the greater amberjack, and also its appropriateness for diversification with difficult marine species. © 2005 Elsevier B.V. All rights reserved. |
Publications
2006 |
Aquaculture, 257 (1-4), pp. 511–524, 2006, ISSN: 00448486. |
Macrofaunal Biodiversity of the Mediterranean Sandy Beach Environment Inproceedings The Mediterranean Coastal Areas from Watershed to the Sea : Interctions and Changes : Proceedings of the Medcore International Conference, pp. 263–274, Firenze, ITALY, 2006, (Publication Title: The Mediterranean Coastal Areas from Watershed to the Sea : Interctions and Changes : Proceedings of the Medcore International Conference Type: Conference Paper). |
MarBEF theme 1: global patterns of marine biodiversity across ecosystems. Progress with theme I data analysis activities Journal Article MarBEF Newsletter, 4 , pp. 3–4, 2006. |
MedOBIS: Biogeographic information system for the eastern Mediterranean and Black Sea Journal Article Marine Ecology Progress Series, 316 , pp. 225–230, 2006, ISSN: 01718630. |
Microbial “life markers” in the deep hypersaline anoxic basins (DHABs) of the South Ionian Sea Inproceedings Thessaloniki, Greece, 2006, (Publication Title: 8th Pan-Hellenic Symposium of Oceanography & Fisheries Type: poster). |
Aquaculture, 255 , pp. 76 – 81, 2006. |
Mol Phylogenet Evol, 39 (3), pp. 734–46, 2006. |
Molluscan diversity along a Mediterranean soft bottom sublittoral ecotone Journal Article Scientia Marina, 70 (4), pp. 573–583, 2006, ISSN: 02148358, (Publisher: CSIC Consejo Superior de Investigaciones Cientificas 2). |
Mortality of steroid-treated sharpsnout sea bream Diplodus puntazzo caused by Ceratomyxa diplodae (Protozoa: Myxosporea). Inproceedings Fifth International Symposium on Aquatic Animal Health, pp. 148, San Francisco, U.S., 2006. |
Multicausal environmental severity: A flexible framework for microtidal sandy beaches and the role of polychaetes as an indicator taxon Journal Article Estuarine, Coastal and Shelf Science, 70 (4), pp. 643–653, 2006, ISSN: 02727714. |
Ontogeny of the digestive tract in shi drum (Umbrina cirrosa L.) reared using the mesocosm larval rearing system Journal Article Aquaculture, 260 (1-4), pp. 357–368, 2006, ISSN: 00448486. |
Origin of PCDDs in Ball Clay assessed with compound-specific chlorine isotope analysis and radiocarbon dating Journal Article Environmental Science and Technology, 40 (12), pp. 3730–3735, 2006, ISSN: 0013936X. |
Oxytetracycline (OTC) uptake following bath treatment in gilthead sea bream (Sparus aurata) Journal Article Aquaculture, 261 , pp. 1151 – 1155, 2006. |
Pathology of Lamellodiscus spp. (Monogenea) parasitizing the gills of sharpsnout seabream and preliminary results of formalin treatment Journal Article Bulletin of the European Association of Fish Pathologists, 26 (5), pp. 196–201, 2006, ISSN: 01080288. |
Aquaculture, 256 (1-4), pp. 443–456, 2006, ISSN: 00448486. |
Permeability effects on breaking waves over submerged rubble mound breakwaters Inproceedings pp. 211–212, 2006, (Publication Title: International Conference ‘Hydroscience-2006’). |
Spectral characterization of two bioaccumulated methoxylated polybrominated diphenyl ethers Journal Article Chemosphere, 62 (2), pp. 197–203, 2006, ISSN: 00456535. |
Stratified prokaryote network in the oxic-anoxic transition of a deep-sea halocline Journal Article Nature, 440 (7081), pp. 203–207, 2006. |
The establishment of the invasive crab Percnon gibbesi (Ħ. Milne Edwards, 1853) (Crustacea: Decapoda: Grapsidae) in Greek waters Journal Article Aquatic Invasions, 1 (3), pp. 133–136, 2006, ISSN: 17986540, (Publisher: Regional Euro-Asian Biological Invasions Centre). |
The Impact of Trawling on Nutrient and Oxygen Fluxes in the Cretan Sea Inproceedings Thessaloniki, Greece, 2006, (Publication Title: 8th Hellenic Symposium on Oceanography and Fisheries). |
The two isoforms of myosin light chain 2 in gilthead sea bream (Sparus aurata); alternative polyadenylation site selection and tissue expression Journal Article Archiv fur Tierzucht, 49 , pp. 92 – 96, 2006. |
Treatment of Microcotyle sp. (Monogenea) on the gills of cage-cultured red porgy, Pagrus pagrus following baths with formalin and mebendazole Journal Article Aquaculture, 251 (2-4), pp. 167–171, 2006, ISSN: 00448486. |
2005 |
Benthic microbial abundance and activities in an intensively trawled ecosystem (Thermaikos Gulf, Aegean Sea) Journal Article Continental Shelf Research, 25 (19-20), pp. 2570–2584, 2005, ISSN: 02784343. |
Continental Shelf Research, 25 (19-20), pp. 2491–2505, 2005, ISSN: 02784343. |
Potential toxicity of resuspended particulate matter and sediments: Environmental samples from the Bay of Banyuls-sur-Mer and Thermaikos Gulf Journal Article Continental Shelf Research, 25 (19-20), pp. 2521–2532, 2005, ISSN: 02784343. |
Osteological development of the vertebral column and of the fins in Diplodus puntazzo (Cetti, 1777) Journal Article Aquaculture, 250 (1-2), pp. 36–46, 2005, ISSN: 00448486. |
Microbial Ecology, 50 (3), pp. 447–462, 2005, ISSN: 0095-3628, 1432-184X. |
Effect of temperature and additional carbon sources on phenol degradation by an indigenous soil Pseudomonad Journal Article Biodegradation, 16 (5), pp. 403–413, 2005, ISSN: 0923-9820, 1572-9729. |
Aquaculture Nutrition, 11 (5), pp. 341–344, 2005, ISSN: 1353-5773, 1365-2095. |
Effect of diet composition on nutrient digestibility and digestive enzyme levels of gilthead sea bream (Sparus aurata L.) Journal Article Aquaculture Research, 36 (13), pp. 1243–1251, 2005, ISSN: 1355-557X, 1365-2109. |
The Atlantic–Mediterranean transition: Discordant genetic patterns in two seabream species, Diplodus puntazzo (Cetti) and Diplodus sargus (L.) Journal Article Molecular Phylogenetics and Evolution, 36 (3), pp. 523–535, 2005, ISSN: 10557903. |
Skin darkness is related to cortisol, but not MSH, content in post-larval Solea senegalensis Journal Article Journal of Fish Biology, 67 (2), pp. 577–581, 2005, ISSN: 0022-1112, 1095-8649. |
Extrachromosomal DNA of the Symbiont Sodalis glossinidius Journal Article Journal of Bacteriology, 187 (14), pp. 5003–5007, 2005, ISSN: 0021-9193, 1098-5530. |
Origin, diversification, and historical biogeography of the genus Trachurus (Perciformes: Carangidae) Journal Article Molecular Phylogenetics and Evolution, 35 (2), pp. 496–507, 2005, ISSN: 10557903. |
Evaluation of candidate probiotic strains for gilthead sea bream larvae (Sparus aurata) using an in vivo approach Journal Article Letters in Applied Microbiology, 40 (4), pp. 274–277, 2005, ISSN: 0266-8254, 1365-2673. |
Links between Geographic Location, Environmental Factors, and Microbial Community Composition in Sediments of the Eastern Mediterranean Sea Journal Article Microbial Ecology, 49 (3), pp. 367–378, 2005, ISSN: 0095-3628, 1432-184X. |
Transposable element polymorphism of Wolbachia in the mosquito Culex pipiens: evidence of genetic diversity, superinfection and recombination Journal Article Molecular Ecology, 14 (5), pp. 1561–1573, 2005, ISSN: 0962-1083, 1365-294X. |
A microsatellite linkage map of the European sea bass Dicentrarchus labrax L. Journal Article Genetics, 170 (4), pp. 1821–1826, 2005, ISSN: 00166731. |
Additional microsatellites for Sparus aurata and cross-species amplification within the Sparidae family Journal Article Molecular Ecology Notes, 5 , pp. 605 – 607, 2005. |
Marine Biology, 148 (2), pp. 319–326, 2005, ISSN: 00253162. |
Reviews in Fish Biology and Fisheries, 15 , pp. 53 – 73, 2005. |
Antimicrobial activity in bacteria isolated from Senegalese sole, Solea senegalensis, fed with natural prey Journal Article Aquaculture Research, 36 (16), pp. 1619–1627, 2005, ISSN: 1355557X. |
Comparative analysis and characterization of expressed sequence tags in gilthead sea bream (Sparus aurata) liver and embryos. Journal Article Aquaculture, 243 (1-4), pp. 69–81, 2005. |
Aquaculture, 246 (1-4), pp. 467–481, 2005, ISSN: 00448486, (Publisher: Elsevier). |
Composition of the organismic assemblage associated with Caretta caretta Journal Article Journal of the Marine Biological Association of the United Kingdom, 85 (2), pp. 257–261, 2005, ISSN: 00253154. |
Contribution of biomass burning to atmospheric polycyclic aromatic hydrocarbons at three european background sites Journal Article Environmental Science and Technology, 39 (9), pp. 2976–2982, 2005, ISSN: 0013936X. |
Design formulae for wave transmission behind floating breakwaters Inproceedings 2005, (Publication Title: XXXI IAHR Congress). |
Effect of dietary water on growth of dentex Dentex dentex Journal Article Fisheries Science, 71 (6), pp. 1243–1248, 2005, ISSN: 09199268. |
Estimating lagoonal biodiversity in Greece: Comparison of rapid assessment techniques Journal Article Helgoland Marine Research, 59 (3), pp. 177–186, 2005, ISSN: 1438387X. |
First results of greater amberjack (Seriola dumerili) larval rearing in mesocosm Journal Article Aquaculture, 250 (1-2), pp. 155–161, 2005, ISSN: 00448486. |