2005 |
Makridis, P; Martins, S; Vercauteren, T; Driessche, Van K; Decamp, O; Dinis, M T Evaluation of candidate probiotic strains for gilthead sea bream larvae (Sparus aurata) using an in vivo approach Journal Article Letters in Applied Microbiology, 40 (4), pp. 274–277, 2005, ISSN: 0266-8254, 1365-2673. @article{makridis_evaluation_2005, title = {Evaluation of candidate probiotic strains for gilthead sea bream larvae (Sparus aurata) using an in vivo approach}, author = {P Makridis and S Martins and T Vercauteren and K Van Driessche and O Decamp and M T Dinis}, url = {http://doi.wiley.com/10.1111/j.1472-765X.2005.01676.x}, doi = {10.1111/j.1472-765X.2005.01676.x}, issn = {0266-8254, 1365-2673}, year = {2005}, date = {2005-04-01}, urldate = {2020-08-18}, journal = {Letters in Applied Microbiology}, volume = {40}, number = {4}, pages = {274--277}, abstract = {AIMS: The aim of this study was to evaluate the effect of six bacterial strains on gilthead sea bream larvae (Sparus aurata). METHODS AND RESULTS: Six bacterial strains isolated from well-performing live food cultures were identified by sequencing fragments of their 16s rDNA genome to the genus level as Cytophaga sp., Roseobacter sp., Ruergeria sp., Paracoccus sp., Aeromonas sp. and Shewanella sp. Survival rates of gilthead sea bream larvae transferred to seawater added these bacterial strains at concentrations of 6 +/- 0.3 x 10(5) bacteria ml(-1) were similar to those of larvae transferred to sterilized seawater and showed an average of 86% at 9 days after hatching, whereas, survival rates of larvae transferred to filtered seawater were lower (P textless 0.05), and showed an average of 39%, 9 days after hatching. CONCLUSION: Several bacterial strains isolated from well-performing live food cultures showed a positive effect for sea bream larvae when compared with filtered seawater. SIGNIFICANCE AND IMPACT OF THE STUDY: The approach used in this study could be applied as an in vivo evaluation method of candidate probiotic strains used in the rearing of marine fish larvae.}, keywords = {}, pubstate = {published}, tppubtype = {article} } AIMS: The aim of this study was to evaluate the effect of six bacterial strains on gilthead sea bream larvae (Sparus aurata). METHODS AND RESULTS: Six bacterial strains isolated from well-performing live food cultures were identified by sequencing fragments of their 16s rDNA genome to the genus level as Cytophaga sp., Roseobacter sp., Ruergeria sp., Paracoccus sp., Aeromonas sp. and Shewanella sp. Survival rates of gilthead sea bream larvae transferred to seawater added these bacterial strains at concentrations of 6 +/- 0.3 x 10(5) bacteria ml(-1) were similar to those of larvae transferred to sterilized seawater and showed an average of 86% at 9 days after hatching, whereas, survival rates of larvae transferred to filtered seawater were lower (P textless 0.05), and showed an average of 39%, 9 days after hatching. CONCLUSION: Several bacterial strains isolated from well-performing live food cultures showed a positive effect for sea bream larvae when compared with filtered seawater. SIGNIFICANCE AND IMPACT OF THE STUDY: The approach used in this study could be applied as an in vivo evaluation method of candidate probiotic strains used in the rearing of marine fish larvae. |
Polymenakou, P N; Bertilsson, S; Tselepides, A; Stephanou, E G Links between Geographic Location, Environmental Factors, and Microbial Community Composition in Sediments of the Eastern Mediterranean Sea Journal Article Microbial Ecology, 49 (3), pp. 367–378, 2005, ISSN: 0095-3628, 1432-184X. @article{polymenakou_links_2005, title = {Links between Geographic Location, Environmental Factors, and Microbial Community Composition in Sediments of the Eastern Mediterranean Sea}, author = {P N Polymenakou and S Bertilsson and A Tselepides and E G Stephanou}, url = {http://link.springer.com/10.1007/s00248-004-0274-5}, doi = {10.1007/s00248-004-0274-5}, issn = {0095-3628, 1432-184X}, year = {2005}, date = {2005-04-01}, urldate = {2020-08-10}, journal = {Microbial Ecology}, volume = {49}, number = {3}, pages = {367--378}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Duron, Olivier; Lagnel, Jacques; Raymond, Michel; Bourtzis, Kostas; Fort, Philippe; Weill, Mylene Transposable element polymorphism of Wolbachia in the mosquito Culex pipiens: evidence of genetic diversity, superinfection and recombination Journal Article Molecular Ecology, 14 (5), pp. 1561–1573, 2005, ISSN: 0962-1083, 1365-294X. @article{duron_transposable_2005, title = {Transposable element polymorphism of Wolbachia in the mosquito Culex pipiens: evidence of genetic diversity, superinfection and recombination}, author = {Olivier Duron and Jacques Lagnel and Michel Raymond and Kostas Bourtzis and Philippe Fort and Mylene Weill}, url = {http://doi.wiley.com/10.1111/j.1365-294X.2005.02495.x}, doi = {10.1111/j.1365-294X.2005.02495.x}, issn = {0962-1083, 1365-294X}, year = {2005}, date = {2005-04-01}, urldate = {2020-08-18}, journal = {Molecular Ecology}, volume = {14}, number = {5}, pages = {1561--1573}, abstract = {Wolbachia is a group of maternally inherited endosymbiotic bacteria that infect and induce cytoplasmic incompatibility (CI) in a wide range of arthropods. In contrast to other species, the mosquito Culex pipiens displays an extremely high number of CI types suggesting differential infection by multiple Wolbachia strains. Attempts so far failed to detect Wolbachia polymorphism that might explain this high level of CI diversity found in C. pipiens populations. Here, we establish that Wolbachia infection is near to or at fixation in worldwide populations of the C. pipiens complex. Wolbachia polymorphism was addressed by sequence analysis of the Tr1 gene, a unique transposable element of the IS5 family, which allowed the identification of five C. pipiens Wolbachia strains, differing either by nucleotide substitution, presence or absence pattern, or insertion site. Sequence analysis also showed that recombination, transposition and superinfection occurred at very low frequencies. Analysis of the geographical distributions of each Wolbachia strain among C. pipiens populations indicated a strong worldwide differentiation independent from mosquito subspecies type, except in the UK. The availability of this polymorphic marker now opens the way to investigate evolution of Wolbachia populations and CI dynamics, in particular in regions where multiple crossing types coexist among C. pipiens populations. © 2005 Blackwell Publishing Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Wolbachia is a group of maternally inherited endosymbiotic bacteria that infect and induce cytoplasmic incompatibility (CI) in a wide range of arthropods. In contrast to other species, the mosquito Culex pipiens displays an extremely high number of CI types suggesting differential infection by multiple Wolbachia strains. Attempts so far failed to detect Wolbachia polymorphism that might explain this high level of CI diversity found in C. pipiens populations. Here, we establish that Wolbachia infection is near to or at fixation in worldwide populations of the C. pipiens complex. Wolbachia polymorphism was addressed by sequence analysis of the Tr1 gene, a unique transposable element of the IS5 family, which allowed the identification of five C. pipiens Wolbachia strains, differing either by nucleotide substitution, presence or absence pattern, or insertion site. Sequence analysis also showed that recombination, transposition and superinfection occurred at very low frequencies. Analysis of the geographical distributions of each Wolbachia strain among C. pipiens populations indicated a strong worldwide differentiation independent from mosquito subspecies type, except in the UK. The availability of this polymorphic marker now opens the way to investigate evolution of Wolbachia populations and CI dynamics, in particular in regions where multiple crossing types coexist among C. pipiens populations. © 2005 Blackwell Publishing Ltd. |
Chistiakov, D A; Hellemans, B; Haley, C S; Law, A S; Tsigenopoulos, C S; Kotoulas, G; Bertotto, D; Libertini, A; Volckaert, F A M A microsatellite linkage map of the European sea bass Dicentrarchus labrax L. Journal Article Genetics, 170 (4), pp. 1821–1826, 2005, ISSN: 00166731. @article{chistiakov_microsatellite_2005, title = {A microsatellite linkage map of the European sea bass Dicentrarchus labrax L.}, author = {D A Chistiakov and B Hellemans and C S Haley and A S Law and C S Tsigenopoulos and G Kotoulas and D Bertotto and A Libertini and F A M Volckaert}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-25444490899&doi=10.1534%2fgenetics.104.039719&partnerID=40&md5=8dfc95ab2fdb751d0c70b2f5f1342b11}, doi = {10.1534/genetics.104.039719}, issn = {00166731}, year = {2005}, date = {2005-01-01}, journal = {Genetics}, volume = {170}, number = {4}, pages = {1821--1826}, abstract = {A genetic linkage map of the European sea bass (Dicentrarchus labrax) was constructed from 174 microsatellite markers, including 145 new markers reported in this study. The mapping panel was derived from farmed sea bass from the North Adriatic Sea and consisted of a single family including both parents and 50 full-sib progeny (biparental diploids). A total of 162 microsatellites were mapped in 25 linkage groups. Eleven loci represent type I (coding) markers; 2 loci are located within the peptide Y (linkage group 1) and cytochrome P450 aromatase (linkage group 6) genes. The sex-averaged map spans 814.5 cM of the sea bass genome. The female map covers 905.9 cM, whereas the male map covers only 567.4 cM. The constructed map represents the first linkage map of European sea bass, one of the most important aquaculture species in Europe. Copyright © 2005 by the Genetics Society of America.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A genetic linkage map of the European sea bass (Dicentrarchus labrax) was constructed from 174 microsatellite markers, including 145 new markers reported in this study. The mapping panel was derived from farmed sea bass from the North Adriatic Sea and consisted of a single family including both parents and 50 full-sib progeny (biparental diploids). A total of 162 microsatellites were mapped in 25 linkage groups. Eleven loci represent type I (coding) markers; 2 loci are located within the peptide Y (linkage group 1) and cytochrome P450 aromatase (linkage group 6) genes. The sex-averaged map spans 814.5 cM of the sea bass genome. The female map covers 905.9 cM, whereas the male map covers only 567.4 cM. The constructed map represents the first linkage map of European sea bass, one of the most important aquaculture species in Europe. Copyright © 2005 by the Genetics Society of America. |
Brown, R C; Tsalavouta, M; Terzoglou, V; Magoulas, A; McAndrew, B J Additional microsatellites for Sparus aurata and cross-species amplification within the Sparidae family Journal Article Molecular Ecology Notes, 5 , pp. 605 – 607, 2005. @article{brown_additional_2005, title = {Additional microsatellites for Sparus aurata and cross-species amplification within the Sparidae family}, author = {R C Brown and M Tsalavouta and V Terzoglou and A Magoulas and B J McAndrew}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-28344455572&partnerID=40&md5=7f56611349fb1cbe98f6e3fbe1c0e1a7}, doi = {10.1111/j.1471-8286.2005.01007.x}, year = {2005}, date = {2005-01-01}, journal = {Molecular Ecology Notes}, volume = {5}, pages = {605 -- 607}, abstract = {Six new microsatellite loci were isolated and characterized in 32 individuals from a farm population of gilthead seabream (Sparus aurata). Expected heterozygosity at all loci was high, ranging from 0.835 to 0.958 with between 10 and 27 alleles per locus. A multiplex polymerase chain reaction protocol was developed using four of the loci. Cross-species amplification of the loci was tested in six species of the Sparidae family and four loci were successfully amplified in two or more related species. © 2005 Blackwell Publishing Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Six new microsatellite loci were isolated and characterized in 32 individuals from a farm population of gilthead seabream (Sparus aurata). Expected heterozygosity at all loci was high, ranging from 0.835 to 0.958 with between 10 and 27 alleles per locus. A multiplex polymerase chain reaction protocol was developed using four of the loci. Cross-species amplification of the loci was tested in six species of the Sparidae family and four loci were successfully amplified in two or more related species. © 2005 Blackwell Publishing Ltd. |
Richardson, C A; Kingsley-Smith, P R; Seed, R; Chatzinikolaou, E Marine Biology, 148 (2), pp. 319–326, 2005, ISSN: 00253162. @article{richardson_age_2005, title = {Age and growth of the naticid gastropod Polinices pulchellus (Gastropoda: Naticidae) based on length frequency analysis and statolith growth rings}, author = {C A Richardson and P R Kingsley-Smith and R Seed and E Chatzinikolaou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-28444483588&doi=10.1007%2fs00227-005-0072-8&partnerID=40&md5=037cebd2b1e1ca9d1ed2d2bbb9e5bc45}, doi = {10.1007/s00227-005-0072-8}, issn = {00253162}, year = {2005}, date = {2005-01-01}, journal = {Marine Biology}, volume = {148}, number = {2}, pages = {319--326}, abstract = {In Red Wharf Bay, UK the naticid gastropod, Polinices pulchellus, was more abundant and more highly aggregated during the summer months (June-August 2001) than during the winter (December 2000). Whilst small numbers of juvenile P. pulchellus (4-6 mm shell length) were present throughout the year the population consisted mainly of individuals of 12-14 mm shell length. Juvenile snails grew rapidly in size during the winter and early spring; growth then virtually ceased between May and June, following which there was a further period of rapid growth between August and February. Densities ranged between 57 and 4,073 ha-1 and the largest individual collected during this investigation measured 16.2 mm in shell length. Statoliths from adult P. pulchellus revealed the presence of a settlement ring and two prominent growth rings (rings 1 and 2). A curvilinear relationship exists between statolith diameter and shell length in snails up to 16 mm in length. Settlement rings ranged in diameter from 19.7 to 45.2 μm (mean 29.8 μm; SE=0.41) giving an estimated shell length of the settled juvenile of 1.1 mm. The diameter of ring 1 and ring 2 were significantly correlated indicating that rapid growth during the first year is maintained during year 2. Shell lengths estimated from the diameters of the prominent statolith rings and those obtained from length frequency data analysis (LFDA), were broadly congruent strongly suggesting an annual periodicity to the statolith rings. The largest snails (>15 mm) present within this population were estimated to be between 2 and 3 years old. Von Bertallanfy seasonal growth curves obtained from the LFDA predicted values of L∞, K and t 0 of 14.32 mm, 1.54 and -0.14 years, respectively, suggesting that P. pulchellus rapidly attains its maximum asymptotic size. © Springer-Verlag 2005.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In Red Wharf Bay, UK the naticid gastropod, Polinices pulchellus, was more abundant and more highly aggregated during the summer months (June-August 2001) than during the winter (December 2000). Whilst small numbers of juvenile P. pulchellus (4-6 mm shell length) were present throughout the year the population consisted mainly of individuals of 12-14 mm shell length. Juvenile snails grew rapidly in size during the winter and early spring; growth then virtually ceased between May and June, following which there was a further period of rapid growth between August and February. Densities ranged between 57 and 4,073 ha-1 and the largest individual collected during this investigation measured 16.2 mm in shell length. Statoliths from adult P. pulchellus revealed the presence of a settlement ring and two prominent growth rings (rings 1 and 2). A curvilinear relationship exists between statolith diameter and shell length in snails up to 16 mm in length. Settlement rings ranged in diameter from 19.7 to 45.2 μm (mean 29.8 μm; SE=0.41) giving an estimated shell length of the settled juvenile of 1.1 mm. The diameter of ring 1 and ring 2 were significantly correlated indicating that rapid growth during the first year is maintained during year 2. Shell lengths estimated from the diameters of the prominent statolith rings and those obtained from length frequency data analysis (LFDA), were broadly congruent strongly suggesting an annual periodicity to the statolith rings. The largest snails (>15 mm) present within this population were estimated to be between 2 and 3 years old. Von Bertallanfy seasonal growth curves obtained from the LFDA predicted values of L∞, K and t 0 of 14.32 mm, 1.54 and -0.14 years, respectively, suggesting that P. pulchellus rapidly attains its maximum asymptotic size. © Springer-Verlag 2005. |
Rigos, G; Troisi, G M Reviews in Fish Biology and Fisheries, 15 , pp. 53 – 73, 2005. @article{rigos_antibacterial_2005, title = {Antibacterial agents in Mediterranean finfish farming: A synopsis of drug pharmacokinetics in important euryhaline fish species and possible environmental implications}, author = {G Rigos and G M Troisi}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-29544446441&partnerID=40&md5=baa5e88d117bde3be196951065389749}, year = {2005}, date = {2005-01-01}, journal = {Reviews in Fish Biology and Fisheries}, volume = {15}, pages = {53 -- 73}, abstract = {The literature pertaining to the use of registered antibacterial agents in Mediterranean finfish farming is reviewed, with an emphasis on the Greek fish-farming industry. This review provides a scientific resource dedicated to the design of future antibacterial dosing regimes in Mediterranean fish farming, where insufficient supporting information is currently available. This paper addresses the paucity in knowledge concerning pharmacokinetics and the efficacy and environmental impact of commonly used antibacterials needed to direct future research and promote good practices in the euryhaline fish farming industry. Several registered antibacterials are currently available for combating bacterial infections, including tetracyclines, (fluoro) quinolones, potentiated sulfa, penicillin and chloramphenicol derivatives. Based on the available data, oxytetracycline (OTC) and quinolone drugs (oxolinic acid - OA and flumequine - FLU) are the most widely used in Mediterranean aquaculture. As a result these drugs have received the most extensive studies, whereas, there is considerable paucity of reliable data on pharmacokinetic and the depletion characteristics of other drugs used, particularly potentiated sulfa, penicillin derivatives and florfenicol. We find there is incomplete data on drug efficacy and minimum inhibitory concentrations (MIC) for common antibacterials used against the major bacterial pathogens of Mediterranean fish species. Furthermore, a considerable lack of data on environmental drug concentrations around Mediterranean fish farms was also identified, highlighting the need for more extensive environmental studies to monitor contamination in environmental components i.e., water and sediment, and in non-target species (flora and fauna). Prudent selection and use of antibacterials can encourage lower dosage applications, enhance treatment efficacy, and help to minimize contamination of the environment. Selection of readily bioavailable drugs which have low environmental persistence, low aquatic toxicity and high antibacterial efficacy is advised, to reduce potential losses to the environment and associated toxic effects on target species and the development of bacterial resistance. Lack of present data made it impossible to provide thorough and accurate guidance on selection and use of antibacterials and approaches for minimizing environmental impacts for the treatment of major euryhaline aquaculture species. © Springer 2005.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The literature pertaining to the use of registered antibacterial agents in Mediterranean finfish farming is reviewed, with an emphasis on the Greek fish-farming industry. This review provides a scientific resource dedicated to the design of future antibacterial dosing regimes in Mediterranean fish farming, where insufficient supporting information is currently available. This paper addresses the paucity in knowledge concerning pharmacokinetics and the efficacy and environmental impact of commonly used antibacterials needed to direct future research and promote good practices in the euryhaline fish farming industry. Several registered antibacterials are currently available for combating bacterial infections, including tetracyclines, (fluoro) quinolones, potentiated sulfa, penicillin and chloramphenicol derivatives. Based on the available data, oxytetracycline (OTC) and quinolone drugs (oxolinic acid - OA and flumequine - FLU) are the most widely used in Mediterranean aquaculture. As a result these drugs have received the most extensive studies, whereas, there is considerable paucity of reliable data on pharmacokinetic and the depletion characteristics of other drugs used, particularly potentiated sulfa, penicillin derivatives and florfenicol. We find there is incomplete data on drug efficacy and minimum inhibitory concentrations (MIC) for common antibacterials used against the major bacterial pathogens of Mediterranean fish species. Furthermore, a considerable lack of data on environmental drug concentrations around Mediterranean fish farms was also identified, highlighting the need for more extensive environmental studies to monitor contamination in environmental components i.e., water and sediment, and in non-target species (flora and fauna). Prudent selection and use of antibacterials can encourage lower dosage applications, enhance treatment efficacy, and help to minimize contamination of the environment. Selection of readily bioavailable drugs which have low environmental persistence, low aquatic toxicity and high antibacterial efficacy is advised, to reduce potential losses to the environment and associated toxic effects on target species and the development of bacterial resistance. Lack of present data made it impossible to provide thorough and accurate guidance on selection and use of antibacterials and approaches for minimizing environmental impacts for the treatment of major euryhaline aquaculture species. © Springer 2005. |
Makridis, P; Martins, S; Tsalavouta, M; Dionisio, L C; Kotoulas, G; Magoulas, A; Dinis, M T Antimicrobial activity in bacteria isolated from Senegalese sole, Solea senegalensis, fed with natural prey Journal Article Aquaculture Research, 36 (16), pp. 1619–1627, 2005, ISSN: 1355557X. @article{makridis_antimicrobial_2005, title = {Antimicrobial activity in bacteria isolated from Senegalese sole, Solea senegalensis, fed with natural prey}, author = {P Makridis and S Martins and M Tsalavouta and L C Dionisio and G Kotoulas and A Magoulas and M T Dinis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-29044440557&doi=10.1111%2fj.1365-2109.2005.01388.x&partnerID=40&md5=64efc6efd5ef382391232a269eb428d1}, doi = {10.1111/j.1365-2109.2005.01388.x}, issn = {1355557X}, year = {2005}, date = {2005-01-01}, journal = {Aquaculture Research}, volume = {36}, number = {16}, pages = {1619--1627}, abstract = {Intestinal bacteria in marine fish may produce antimicrobial substances which inhibit pathogenic bacteria. The aim of this study was to determine the influence of a change of fish diet on the antimicrobial activity of the culturable aerobic gut microflora of Senegalese sole, Solea senegalensis. Pre-adult 15-month-old fish previously fed on an artificial diet, were fed polychaetes (Hediste diversicolor), which form part of the natural diet of Senegalese sole. Samples were taken 0, 3 and 6 weeks after start of the experiment from the stomach, small and large intestine of the fish. The bacterial strains isolated from these samples were sub-cultured to pure cultures and stored at - 80°C. Several biochemical tests were run to obtain some basic phenotypic characteristics of the isolated strains. Amplification and sequencing of 16S rDNA fragments were used to identify the majority of the bacterial strains isolated. The identification by use of this molecular approach gave results in agreement to the phenotypic characterization. Feeding with polychaetes significantly increased (P textless 0.05) the numbers of presumptive Vibrio isolates in the gut. The number of bacterial strains with antimicrobial activity, as determined by two in vitro approaches, was significantly (P textless 0.05) increased by feeding with polychaetes. © 2005 Blackwell Publishing Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Intestinal bacteria in marine fish may produce antimicrobial substances which inhibit pathogenic bacteria. The aim of this study was to determine the influence of a change of fish diet on the antimicrobial activity of the culturable aerobic gut microflora of Senegalese sole, Solea senegalensis. Pre-adult 15-month-old fish previously fed on an artificial diet, were fed polychaetes (Hediste diversicolor), which form part of the natural diet of Senegalese sole. Samples were taken 0, 3 and 6 weeks after start of the experiment from the stomach, small and large intestine of the fish. The bacterial strains isolated from these samples were sub-cultured to pure cultures and stored at - 80°C. Several biochemical tests were run to obtain some basic phenotypic characteristics of the isolated strains. Amplification and sequencing of 16S rDNA fragments were used to identify the majority of the bacterial strains isolated. The identification by use of this molecular approach gave results in agreement to the phenotypic characterization. Feeding with polychaetes significantly increased (P textless 0.05) the numbers of presumptive Vibrio isolates in the gut. The number of bacterial strains with antimicrobial activity, as determined by two in vitro approaches, was significantly (P textless 0.05) increased by feeding with polychaetes. © 2005 Blackwell Publishing Ltd. |
Sarropoulou, Elena; Power, D M; A, Magoulas; Geisler, R; Kotoulas, Giorgios Comparative analysis and characterization of expressed sequence tags in gilthead sea bream (Sparus aurata) liver and embryos. Journal Article Aquaculture, 243 (1-4), pp. 69–81, 2005. @article{sarropoulou_comparative_2005, title = {Comparative analysis and characterization of expressed sequence tags in gilthead sea bream (Sparus aurata) liver and embryos.}, author = {Elena Sarropoulou and D M Power and Magoulas A and R Geisler and Giorgios Kotoulas}, year = {2005}, date = {2005-01-01}, journal = {Aquaculture}, volume = {243}, number = {1-4}, pages = {69--81}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Mylonas, C C; Cardinaletti, G; Sigelaki, I; Polzonetti-Magni, A Aquaculture, 246 (1-4), pp. 467–481, 2005, ISSN: 00448486, (Publisher: Elsevier). @article{mylonas_comparative_2005, title = {Comparative efficacy of clove oil and 2-phenoxyethanol as anesthetics in the aquaculture of European sea bass (Dicentrarchus labrax) and gilthead sea bream (Sparus aurata) at different temperatures}, author = {C C Mylonas and G Cardinaletti and I Sigelaki and A Polzonetti-Magni}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-17944372332&doi=10.1016%2fj.aquaculture.2005.02.046&partnerID=40&md5=c28d66ffa819306390f68fb47b57ced5}, doi = {10.1016/j.aquaculture.2005.02.046}, issn = {00448486}, year = {2005}, date = {2005-01-01}, journal = {Aquaculture}, volume = {246}, number = {1-4}, pages = {467--481}, abstract = {The efficacy of clove oil as an anesthetic was evaluated in juvenile European sea bass (Dicentrarchus labrax) and gilthead sea bream (Sparus aurata), and was compared to the commonly used 2-phenoxyethanol through a series of experiments simulating aquaculture activities. Firstly, using as a criterion the acquisition of complete anesthesia (stage A5) in < 3 min and recovery (stage R5) in < 10 min, the optimal doses at 25°C were determined to be 40 mg l-1 of clove oil for both species, and 350 mg l-1 and 300 mg l-1 of 2-phenoxyethanol for European sea bass and gilthead sea bream, respectively. At 15°C, the optimal doses for the European sea bass were determined to be around 30 mg l-1 clove oil and 300 mg l -1 2-phenoxyethanol, and for gilthead sea bream 55 mg l-1 clove oil and 450 mg l-1 2-phenoxyethanol. Increasing the exposure time of fish to the optimal anesthetic dose for 5, 10 or 15 min after stage A5 anesthesia prolonged recovery time (ANOVA, P < 0.001), especially in gilthead sea bream, which also suffered significant mortality (10-83%). As expected, the lower temperature resulted in significantly longer anesthesia induction and recovery times (ANOVA, P < 0.001), presumably due to the positive relationship between temperature, and opercular ventilation rates (ANOVA, P < 0.001) and metabolism. Finally, repeated exposure to anesthetics at 0 h, 3 h and 24 h increased significantly the induction time to stage A5 anesthesia (ANOVA, P < 0.001), suggesting the development of a slight tolerance, especially to the clove oil. The study demonstrated that clove oil can be used as an effective anesthetic in European sea bass and gilthead sea bream aquaculture, at almost 10-fold lower doses than 2-phenoxyethanol. The observed differences in (a) dose response, (b) anesthesia induction and recovery times, (c) ventilation rates and (d) mortality after prolonged exposure among the two species, underscore the need to undertake extensive studies with the specific fish species, anesthetic and experimental procedure employed, before clove oil or any other anesthetic is proposed for commercial use in an aquaculture species. © 2005 Elsevier B.V. All rights reserved.}, note = {Publisher: Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {article} } The efficacy of clove oil as an anesthetic was evaluated in juvenile European sea bass (Dicentrarchus labrax) and gilthead sea bream (Sparus aurata), and was compared to the commonly used 2-phenoxyethanol through a series of experiments simulating aquaculture activities. Firstly, using as a criterion the acquisition of complete anesthesia (stage A5) in < 3 min and recovery (stage R5) in < 10 min, the optimal doses at 25°C were determined to be 40 mg l-1 of clove oil for both species, and 350 mg l-1 and 300 mg l-1 of 2-phenoxyethanol for European sea bass and gilthead sea bream, respectively. At 15°C, the optimal doses for the European sea bass were determined to be around 30 mg l-1 clove oil and 300 mg l -1 2-phenoxyethanol, and for gilthead sea bream 55 mg l-1 clove oil and 450 mg l-1 2-phenoxyethanol. Increasing the exposure time of fish to the optimal anesthetic dose for 5, 10 or 15 min after stage A5 anesthesia prolonged recovery time (ANOVA, P < 0.001), especially in gilthead sea bream, which also suffered significant mortality (10-83%). As expected, the lower temperature resulted in significantly longer anesthesia induction and recovery times (ANOVA, P < 0.001), presumably due to the positive relationship between temperature, and opercular ventilation rates (ANOVA, P < 0.001) and metabolism. Finally, repeated exposure to anesthetics at 0 h, 3 h and 24 h increased significantly the induction time to stage A5 anesthesia (ANOVA, P < 0.001), suggesting the development of a slight tolerance, especially to the clove oil. The study demonstrated that clove oil can be used as an effective anesthetic in European sea bass and gilthead sea bream aquaculture, at almost 10-fold lower doses than 2-phenoxyethanol. The observed differences in (a) dose response, (b) anesthesia induction and recovery times, (c) ventilation rates and (d) mortality after prolonged exposure among the two species, underscore the need to undertake extensive studies with the specific fish species, anesthetic and experimental procedure employed, before clove oil or any other anesthetic is proposed for commercial use in an aquaculture species. © 2005 Elsevier B.V. All rights reserved. |
Kitsos, M -S; Christodoulou, M; Arvanitidis, C; Mavidis, M; Kirmitzoglou, I; Koukouras, A Composition of the organismic assemblage associated with Caretta caretta Journal Article Journal of the Marine Biological Association of the United Kingdom, 85 (2), pp. 257–261, 2005, ISSN: 00253154. @article{kitsos_composition_2005, title = {Composition of the organismic assemblage associated with Caretta caretta}, author = {M -S Kitsos and M Christodoulou and C Arvanitidis and M Mavidis and I Kirmitzoglou and A Koukouras}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-18244363640&doi=10.1017%2fS0025315405011136h&partnerID=40&md5=557179d4cedaf466179b5740074433b9}, doi = {10.1017/S0025315405011136h}, issn = {00253154}, year = {2005}, date = {2005-01-01}, journal = {Journal of the Marine Biological Association of the United Kingdom}, volume = {85}, number = {2}, pages = {257--261}, abstract = {The organismic assemblage associated with Caretta caretta was studied in 37 loggerhead turtles, washed ashore dead at various localities in the northern Aegean Sea. A total number of 63 macrobenthic species and 17 species of macroalgae were identified. From these species, 41 and 13 respectively are reported for the first time as epibionts of this turtle. Analysis of the epibiont species distribution on six loggerhead turtles gave some evidence regarding the settlement preferences of certain epibiont species. From a biogeographical point of view, 61.3% of the epibiont species were cosmopolitan, 30.7% had an Atlanta-Mediterranean distribution, while 8% were Mediterranean endemics.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The organismic assemblage associated with Caretta caretta was studied in 37 loggerhead turtles, washed ashore dead at various localities in the northern Aegean Sea. A total number of 63 macrobenthic species and 17 species of macroalgae were identified. From these species, 41 and 13 respectively are reported for the first time as epibionts of this turtle. Analysis of the epibiont species distribution on six loggerhead turtles gave some evidence regarding the settlement preferences of certain epibiont species. From a biogeographical point of view, 61.3% of the epibiont species were cosmopolitan, 30.7% had an Atlanta-Mediterranean distribution, while 8% were Mediterranean endemics. |
Mandalakis, M; Gustafsson, Ö; Alsberg, T; Egebäck, A -L; Reddy, C M; Xu, L; Klanova, J; Holoubek, I; Stephanou, E G Contribution of biomass burning to atmospheric polycyclic aromatic hydrocarbons at three european background sites Journal Article Environmental Science and Technology, 39 (9), pp. 2976–2982, 2005, ISSN: 0013936X. @article{mandalakis_contribution_2005, title = {Contribution of biomass burning to atmospheric polycyclic aromatic hydrocarbons at three european background sites}, author = {M Mandalakis and Ö Gustafsson and T Alsberg and A -L Egebäck and C M Reddy and L Xu and J Klanova and I Holoubek and E G Stephanou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-18344396135&doi=10.1021%2fes048184v&partnerID=40&md5=e821f5d70d448ac54af11b534fa61cfa}, doi = {10.1021/es048184v}, issn = {0013936X}, year = {2005}, date = {2005-01-01}, journal = {Environmental Science and Technology}, volume = {39}, number = {9}, pages = {2976--2982}, abstract = {Radiocarbon analysis of atmospheric polycyclic aromatic hydrocarbons (PAHs) from three background areas in Sweden, Croatia, and Greece was performed to apportion their origin between fossil and biomass combustion. Diagnostic ratios of PAHs implied that wood and coal combustion was relatively more important in the northern European site, while combustion of fossil fuels was the dominant source of PAHs to the two central-southern European background sites. The radiocarbon content (Δ14C) of atmospheric PAHs in Sweden ranged between -388‰ and -381‰, while more depleted values were observed for Greece (-914‰) and Croatia (-888‰). Using a 14C isotopic mass balance model it was calculated that biomass burning contributes nearly 10% of the total PAH burden in the studied southern European atmosphere with fossil fuel combustion making up the 90% balance. In contrast, biomass burning contributes about 50% of total PAHs in the atmosphere at the Swedish site. Our results suggest that the relative contributions of biomass burning and fossil fuels to atmospheric PAHs may differ considerably between countries, and therefore, different national control strategies might be needed if a further reduction of these pollutants is to be achieved on a continental-global scale. © 2005 American Chemical Society.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Radiocarbon analysis of atmospheric polycyclic aromatic hydrocarbons (PAHs) from three background areas in Sweden, Croatia, and Greece was performed to apportion their origin between fossil and biomass combustion. Diagnostic ratios of PAHs implied that wood and coal combustion was relatively more important in the northern European site, while combustion of fossil fuels was the dominant source of PAHs to the two central-southern European background sites. The radiocarbon content (Δ14C) of atmospheric PAHs in Sweden ranged between -388‰ and -381‰, while more depleted values were observed for Greece (-914‰) and Croatia (-888‰). Using a 14C isotopic mass balance model it was calculated that biomass burning contributes nearly 10% of the total PAH burden in the studied southern European atmosphere with fossil fuel combustion making up the 90% balance. In contrast, biomass burning contributes about 50% of total PAHs in the atmosphere at the Swedish site. Our results suggest that the relative contributions of biomass burning and fossil fuels to atmospheric PAHs may differ considerably between countries, and therefore, different national control strategies might be needed if a further reduction of these pollutants is to be achieved on a continental-global scale. © 2005 American Chemical Society. |
Koutandos, E; Prinos, P Design formulae for wave transmission behind floating breakwaters Inproceedings 2005, (Publication Title: XXXI IAHR Congress). @inproceedings{koutandos_design_2005, title = {Design formulae for wave transmission behind floating breakwaters}, author = {E Koutandos and P Prinos}, year = {2005}, date = {2005-01-01}, volume = {1}, note = {Publication Title: XXXI IAHR Congress}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Chatzifotis, S; Papadakis, I E; Divanach, P Effect of dietary water on growth of dentex Dentex dentex Journal Article Fisheries Science, 71 (6), pp. 1243–1248, 2005, ISSN: 09199268. @article{chatzifotis_effect_2005, title = {Effect of dietary water on growth of dentex Dentex dentex}, author = {S Chatzifotis and I E Papadakis and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-31144439969&doi=10.1111%2fj.1444-2906.2005.01089.x&partnerID=40&md5=8d72e22c36d27bfa837cdc0365529fd7}, doi = {10.1111/j.1444-2906.2005.01089.x}, issn = {09199268}, year = {2005}, date = {2005-01-01}, journal = {Fisheries Science}, volume = {71}, number = {6}, pages = {1243--1248}, abstract = {This study is concerned with the effect of hyrdated diets on growth and feed performance of Dentex dentex. Three levels of dietary water were tested: 7%, 20% and 40%. For the 7% level a commercial feed was used and the 20% and 40% water levels were obtained after adding an appropriate amount of deionized water to the commercial feed and mixing the pellets well. The diets were offered to fish by self-feeders, by which fish were allowed continuous access to diets after activating the self-feeders; by belt feeders, by which the diets were fed continuously and in excess over a 12 h interval; and by hand to apparent satiation two times a day. After 151 days of feeding, the group fed on the 20%-water diet showed significantly (P textless 0.05) higher growth rate than the 7 and 40% groups, while no significant differences were observed in feed conversion rate or daily feed consumption. The means of feed delivery did not influence growth rate. Self-feeder, belt and hand feeding all gave comparable growth. Fish fed the more condensed 7%-water diet had smaller stomachs than fish fed the larger volume 40%-water diet. In the stomach, all groups of fish hydrated their digesta within 2 h after feeding to a moisture content of around 60%. Apparently no difference in digestive process was observed between groups. It was concluded that the addition of water in diet at the level of 20% has a beneficial effect on dentex growth.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study is concerned with the effect of hyrdated diets on growth and feed performance of Dentex dentex. Three levels of dietary water were tested: 7%, 20% and 40%. For the 7% level a commercial feed was used and the 20% and 40% water levels were obtained after adding an appropriate amount of deionized water to the commercial feed and mixing the pellets well. The diets were offered to fish by self-feeders, by which fish were allowed continuous access to diets after activating the self-feeders; by belt feeders, by which the diets were fed continuously and in excess over a 12 h interval; and by hand to apparent satiation two times a day. After 151 days of feeding, the group fed on the 20%-water diet showed significantly (P textless 0.05) higher growth rate than the 7 and 40% groups, while no significant differences were observed in feed conversion rate or daily feed consumption. The means of feed delivery did not influence growth rate. Self-feeder, belt and hand feeding all gave comparable growth. Fish fed the more condensed 7%-water diet had smaller stomachs than fish fed the larger volume 40%-water diet. In the stomach, all groups of fish hydrated their digesta within 2 h after feeding to a moisture content of around 60%. Apparently no difference in digestive process was observed between groups. It was concluded that the addition of water in diet at the level of 20% has a beneficial effect on dentex growth. |
Arvanitidis, C; Chatzigeorgiou, G; Koutsoubas, D; Kevrekidis, T; Dounas, C; Eleftheriou, A; Koulouri, P; Mogias, A Estimating lagoonal biodiversity in Greece: Comparison of rapid assessment techniques Journal Article Helgoland Marine Research, 59 (3), pp. 177–186, 2005, ISSN: 1438387X. @article{arvanitidis_estimating_2005, title = {Estimating lagoonal biodiversity in Greece: Comparison of rapid assessment techniques}, author = {C Arvanitidis and G Chatzigeorgiou and D Koutsoubas and T Kevrekidis and C Dounas and A Eleftheriou and P Koulouri and A Mogias}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-24944557295&doi=10.1007%2fs10152-005-0216-8&partnerID=40&md5=b9d9a7372762c2833f2e6ea1c70d270e}, doi = {10.1007/s10152-005-0216-8}, issn = {1438387X}, year = {2005}, date = {2005-01-01}, journal = {Helgoland Marine Research}, volume = {59}, number = {3}, pages = {177--186}, abstract = {An attempt is made to compare the results of different rapid biodiversity assessment techniques at the pan-Mediterranean, sectorial and local levels. A uniform multivariate pattern exists at the pan-Mediterranean and national (sectorial) levels: lagoons can be different when they host only a few species, but as species numbers increase, lagoons become homogenous in composition. Multivariate techniques cannot distinguish anthropogenically-impacted lagoons from those, which are naturally disturbed. In the pan-Mediterranean context it is the higher taxonomic levels, but in the national and local context it is the most abundant macrobenthic groups (polychaetes, molluscs and crustaceans) and meiobenthos which provide patterns closest to that derived from the species level. Taxonomic distinctness indices applied to polychaete and mollusc inventories provide meaningful results at most levels and scales of observation. These indices seem to be robust enough to discriminate anthropogenically impacted from naturally disturbed lagoons. © Springer-Verlag and AWI 2005.}, keywords = {}, pubstate = {published}, tppubtype = {article} } An attempt is made to compare the results of different rapid biodiversity assessment techniques at the pan-Mediterranean, sectorial and local levels. A uniform multivariate pattern exists at the pan-Mediterranean and national (sectorial) levels: lagoons can be different when they host only a few species, but as species numbers increase, lagoons become homogenous in composition. Multivariate techniques cannot distinguish anthropogenically-impacted lagoons from those, which are naturally disturbed. In the pan-Mediterranean context it is the higher taxonomic levels, but in the national and local context it is the most abundant macrobenthic groups (polychaetes, molluscs and crustaceans) and meiobenthos which provide patterns closest to that derived from the species level. Taxonomic distinctness indices applied to polychaete and mollusc inventories provide meaningful results at most levels and scales of observation. These indices seem to be robust enough to discriminate anthropogenically impacted from naturally disturbed lagoons. © Springer-Verlag and AWI 2005. |
Papandroulakis, N; Mylonas, C C; Maingot, E; Divanach, P First results of greater amberjack (Seriola dumerili) larval rearing in mesocosm Journal Article Aquaculture, 250 (1-2), pp. 155–161, 2005, ISSN: 00448486. @article{papandroulakis_first_2005, title = {First results of greater amberjack (Seriola dumerili) larval rearing in mesocosm}, author = {N Papandroulakis and C C Mylonas and E Maingot and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-27344450721&doi=10.1016%2fj.aquaculture.2005.02.036&partnerID=40&md5=85aba7698333eb1cdf77b60229a40d1b}, doi = {10.1016/j.aquaculture.2005.02.036}, issn = {00448486}, year = {2005}, date = {2005-01-01}, journal = {Aquaculture}, volume = {250}, number = {1-2}, pages = {155--161}, abstract = {Species diversification is considered a major approach for the sustainable development of aquaculture. The greater amberjack (Seriola dumerili) has particular characteristics-advantages making it an appropriate candidate: high growth rate, large size, and established worldwide market. In the present study, the mesocosm method for larval rearing was applied, since it has been shown to be effective in the larval rearing of several species so far. This method is a semi-intensive technology, based on daily exogenous food addition, but also having the capacity of some endogenous productivity. Greater amberjack eggs were obtained from wild-caught fish matured in captivity in 6 years, after induced spawning with implants containing gonadotropin-releasing hormone agonist (GnRHa). A total of 9800 eggs survived after embryo appearance and were incubated in a 40-m3 tank with natural seawater of 40 psu. Rearing lasted 40 days. After mouth opening on day 2 post hatching, exogenous feeding with rotifers, Artemia nauplii and inert feed was initiated, while endogenous produced copepods contributed as food for the larvae from day 7 post hatching onwards. During rearing, larvae grew with an exponential rate of 0.073 day -1 in terms of total length (TL), and reached 39.9 ± 5.4 mm and 0.5 ± 0.1 g body weight at the end of the trial. All larvae inflated their swim bladder and completed metamorphosis at about 5 mm and 8 mm TL, respectively. Schooling behavior was first observed when larvae reached 9-10 mm TL, while aggression against the smallest individuals was first noticed the same period. The final population of about 350 individuals (3.5% survival) was transferred at the end of the trial for subsequent on-growing. The results obtained indicate the reliability of the technology for the larval rearing of the greater amberjack, and also its appropriateness for diversification with difficult marine species. © 2005 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Species diversification is considered a major approach for the sustainable development of aquaculture. The greater amberjack (Seriola dumerili) has particular characteristics-advantages making it an appropriate candidate: high growth rate, large size, and established worldwide market. In the present study, the mesocosm method for larval rearing was applied, since it has been shown to be effective in the larval rearing of several species so far. This method is a semi-intensive technology, based on daily exogenous food addition, but also having the capacity of some endogenous productivity. Greater amberjack eggs were obtained from wild-caught fish matured in captivity in 6 years, after induced spawning with implants containing gonadotropin-releasing hormone agonist (GnRHa). A total of 9800 eggs survived after embryo appearance and were incubated in a 40-m3 tank with natural seawater of 40 psu. Rearing lasted 40 days. After mouth opening on day 2 post hatching, exogenous feeding with rotifers, Artemia nauplii and inert feed was initiated, while endogenous produced copepods contributed as food for the larvae from day 7 post hatching onwards. During rearing, larvae grew with an exponential rate of 0.073 day -1 in terms of total length (TL), and reached 39.9 ± 5.4 mm and 0.5 ± 0.1 g body weight at the end of the trial. All larvae inflated their swim bladder and completed metamorphosis at about 5 mm and 8 mm TL, respectively. Schooling behavior was first observed when larvae reached 9-10 mm TL, while aggression against the smallest individuals was first noticed the same period. The final population of about 350 individuals (3.5% survival) was transferred at the end of the trial for subsequent on-growing. The results obtained indicate the reliability of the technology for the larval rearing of the greater amberjack, and also its appropriateness for diversification with difficult marine species. © 2005 Elsevier B.V. All rights reserved. |
Koutandos, E; Prinos, P; Gironella, X Floating breakwaters under regular and irregular wave forcing: reflection and transmission characteristics Journal Article Journal of Hydraulic Research, 43 (2), pp. 174–188, 2005, ISSN: 0022-1686, 1814-2079. @article{koutandos_floating_2005, title = {Floating breakwaters under regular and irregular wave forcing: reflection and transmission characteristics}, author = {E Koutandos and P Prinos and X Gironella}, url = {https://www.tandfonline.com/doi/full/10.1080/00221686.2005.9641234}, doi = {10.1080/00221686.2005.9641234}, issn = {0022-1686, 1814-2079}, year = {2005}, date = {2005-01-01}, urldate = {2020-08-18}, journal = {Journal of Hydraulic Research}, volume = {43}, number = {2}, pages = {174--188}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Koutandos, E; Prinos, P Flow through a porous rubble mound weir Inproceedings pp. 2536–2542, 2005, (Publication Title: XXXI IAHR Congress). @inproceedings{koutandos_flow_2005, title = {Flow through a porous rubble mound weir}, author = {E Koutandos and P Prinos}, year = {2005}, date = {2005-01-01}, volume = {1}, pages = {2536--2542}, note = {Publication Title: XXXI IAHR Congress}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Sarropoulou, E; Kotoulas, G; Power, D M; Geisler, R Physiological Genomics, 23 (2), pp. 182–191, 2005, ISSN: 10948341. @article{sarropoulou_gene_2005, title = {Gene expression profiling of gilthead sea bream during early development and detection of stress-related genes by the application of cDNA microarray technology}, author = {E Sarropoulou and G Kotoulas and D M Power and R Geisler}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33745678365&doi=10.1152%2fphysiolgenomics.00139.2005&partnerID=40&md5=497797e3b9a6bc0c8a3554c078ea6805}, doi = {10.1152/physiolgenomics.00139.2005}, issn = {10948341}, year = {2005}, date = {2005-01-01}, journal = {Physiological Genomics}, volume = {23}, number = {2}, pages = {182--191}, abstract = {Large-scale gene expression studies were performed for one of the main European aquaculture species, the gilthead sea bream Sparus auratus L. For this purpose, a cDNA microarray containing 10,176 clones from a cDNA library of mixed embryonic and larval stages was constructed. In addition to its importance for aquaculture, the taxonomic position and the relatively small genome size of sea bream makes it a prospective model for evolutionary biology and comparative genomics. However, so far, no large-scale analysis of gene expression exists for this species. In the present study, gene expression was analyzed in gilthead sea bream during early development, a significant period in the determination of quantitative traits and therefore of considerable interest for aquaculture. Synexpression groups expressed primarily early and late in development were determined and were composed of both known and novel genes. Furthermore, it was possible to identify stress response genes induced by cortisol injections using the cDNA microarray generated. The creation of gene expression profiles for sea bream by microarray hybridization will accelerate identification of candidate genes involved in multifactorial traits and certain regulatory pathways and will also contribute to a better understanding of the genetic background of fish physiology, which may help to improve aquaculture practices. Copyright © 2005 the American Physiological Society.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Large-scale gene expression studies were performed for one of the main European aquaculture species, the gilthead sea bream Sparus auratus L. For this purpose, a cDNA microarray containing 10,176 clones from a cDNA library of mixed embryonic and larval stages was constructed. In addition to its importance for aquaculture, the taxonomic position and the relatively small genome size of sea bream makes it a prospective model for evolutionary biology and comparative genomics. However, so far, no large-scale analysis of gene expression exists for this species. In the present study, gene expression was analyzed in gilthead sea bream during early development, a significant period in the determination of quantitative traits and therefore of considerable interest for aquaculture. Synexpression groups expressed primarily early and late in development were determined and were composed of both known and novel genes. Furthermore, it was possible to identify stress response genes induced by cortisol injections using the cDNA microarray generated. The creation of gene expression profiles for sea bream by microarray hybridization will accelerate identification of candidate genes involved in multifactorial traits and certain regulatory pathways and will also contribute to a better understanding of the genetic background of fish physiology, which may help to improve aquaculture practices. Copyright © 2005 the American Physiological Society. |
Papadaki, M; Piferrer, F; Zanuy, S; Maingot, E; Divanach, P; Mylonas, C C Journal of Fish Biology, 66 (4), pp. 938–956, 2005, ISSN: 00221112, (Publisher: Blackwell Publishing Ltd). @article{papadaki_growth_2005, title = {Growth, sex differentiation and gonad and plasma levels of sex steroids in male- and female-dominant populations of Dicentrarchus labrax obtained through repeated size grading}, author = {M Papadaki and F Piferrer and S Zanuy and E Maingot and P Divanach and C C Mylonas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-17244363508&doi=10.1111%2fj.0022-1112.2005.00639.x&partnerID=40&md5=818526ad19bf4809e7b643ad51c39425}, doi = {10.1111/j.0022-1112.2005.00639.x}, issn = {00221112}, year = {2005}, date = {2005-01-01}, journal = {Journal of Fish Biology}, volume = {66}, number = {4}, pages = {938--956}, abstract = {Starting from 66 days post hatching (dph), European sea bass Dicentrarchus labrax were graded successively to create a fast growing (L-extreme) and a slow growing (S-extreme) population. The L-extreme population grew significantly larger (ANOVA}, note = {Publisher: Blackwell Publishing Ltd}, keywords = {}, pubstate = {published}, tppubtype = {article} } Starting from 66 days post hatching (dph), European sea bass Dicentrarchus labrax were graded successively to create a fast growing (L-extreme) and a slow growing (S-extreme) population. The L-extreme population grew significantly larger (ANOVA |
Bahri-Sfar, Lilia; Lemaire, Christophe; Chatain, Béatrice; Divanach, Pascal; Hassine, Oum Kalthoum Ben; Bonhomme, François Impact of aquaculture on the genetic structure of mediterranean populations of Dicentrarchus labrax Journal Article Aquatic Living Resources, 18 (1), pp. 71–76, 2005, ISSN: 0990-7440, 1765-2952, (Original title (in French): Impact de l'élevage sur la structure génétique des populations méditerranéennes de Dicentrarchus labrax). @article{bahri-sfar_impact_2005, title = {Impact of aquaculture on the genetic structure of mediterranean populations of Dicentrarchus labrax}, author = {Lilia Bahri-Sfar and Christophe Lemaire and Béatrice Chatain and Pascal Divanach and Oum Kalthoum Ben Hassine and François Bonhomme}, url = {http://www.alr-journal.org/10.1051/alr:2005007}, doi = {10.1051/alr:2005007}, issn = {0990-7440, 1765-2952}, year = {2005}, date = {2005-01-01}, urldate = {2020-08-18}, journal = {Aquatic Living Resources}, volume = {18}, number = {1}, pages = {71--76}, note = {Original title (in French): Impact de l'élevage sur la structure génétique des populations méditerranéennes de Dicentrarchus labrax}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Kokokiris, L; Canario, A; Mylonas, C; Pavlidis, M; Kentouri, M; Divanach, P Induction of ovulation and spawning in the Mediterranean Red Porgy, Pagrus Pagrus, by controlled delivery and acute injection of GnRHa Journal Article Israeli Journal of Aquaculture - Bamidgeh, 57 (4), pp. 223–230, 2005, ISSN: 0792156X. @article{kokokiris_induction_2005, title = {Induction of ovulation and spawning in the Mediterranean Red Porgy, Pagrus Pagrus, by controlled delivery and acute injection of GnRHa}, author = {L Kokokiris and A Canario and C Mylonas and M Pavlidis and M Kentouri and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-29744437550&partnerID=40&md5=fe674a5bea66c6aa820eb3a5b7d62661}, issn = {0792156X}, year = {2005}, date = {2005-01-01}, journal = {Israeli Journal of Aquaculture - Bamidgeh}, volume = {57}, number = {4}, pages = {223--230}, abstract = {Gonadotropin-releasing hormone analogue (GnRHa) in the form of saline injections or sustained-release microspheres was used to induce oocyte maturation, ovulation, and spawning in captive red porgy (Pagrus pagrus). Individually tagged vitellogenic females (n = 9 or 10) were treated at the beginning of the spawning season (March) with 20 μg/kg body weight (bw) GnRHa-loaded microspheres, a single injection of 20 μg/ kg bw dissolved in saline, or physiological saline (control). Females were placed in tanks (one tank per treatment) connected to overflow egg collectors and monitored for 11 days. In addition to the eggs collected from the tank overflow, eggs were stripped from the fish on a daily basis. Only one spawn was obtained from the control fish, probably from a single female, given the small relative fecundity (700 eggs/kg bw). On the contrary, treatment with a GnRHa injection produced two spawns (9 and 11 days after treatment) and 50% of the fish ovulated. Treatment with GnRHa microspheres induced seven spawns (3 and 6-11 days after treatment) and 100% of the females ovulated. Females did not spawn all the eggs ovulated on a particular day, evident from the significant number of eggs obtained by manual stripping. Egg quality did not significantly differ among treatments, whereas number of spawned eggs and total relative fecundity were significantly higher in fish treated with GnRHa microspheres (ANOVA, ptextless0.05). The results demonstrate the potential of GnRHa-loaded microspheres to induce spawning in red porgy, as a method of overcoming spawning failures in commercial hatcheries.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Gonadotropin-releasing hormone analogue (GnRHa) in the form of saline injections or sustained-release microspheres was used to induce oocyte maturation, ovulation, and spawning in captive red porgy (Pagrus pagrus). Individually tagged vitellogenic females (n = 9 or 10) were treated at the beginning of the spawning season (March) with 20 μg/kg body weight (bw) GnRHa-loaded microspheres, a single injection of 20 μg/ kg bw dissolved in saline, or physiological saline (control). Females were placed in tanks (one tank per treatment) connected to overflow egg collectors and monitored for 11 days. In addition to the eggs collected from the tank overflow, eggs were stripped from the fish on a daily basis. Only one spawn was obtained from the control fish, probably from a single female, given the small relative fecundity (700 eggs/kg bw). On the contrary, treatment with a GnRHa injection produced two spawns (9 and 11 days after treatment) and 50% of the fish ovulated. Treatment with GnRHa microspheres induced seven spawns (3 and 6-11 days after treatment) and 100% of the females ovulated. Females did not spawn all the eggs ovulated on a particular day, evident from the significant number of eggs obtained by manual stripping. Egg quality did not significantly differ among treatments, whereas number of spawned eggs and total relative fecundity were significantly higher in fish treated with GnRHa microspheres (ANOVA, ptextless0.05). The results demonstrate the potential of GnRHa-loaded microspheres to induce spawning in red porgy, as a method of overcoming spawning failures in commercial hatcheries. |
Mylonas, C C; Anezaki, L; Divanach, P; Zanuy, S; Piferrer, F; Ron, B; Peduel, A; Atia, Ben I; Gorshkov, S; Tandler, A Journal of Fish Biology, 67 (3), pp. 652–668, 2005, ISSN: 00221112. @article{mylonas_influence_2005, title = {Influence of rearing temperature during the larval and nursery periods on growth and sex differentiation in two Mediterranean strains of Dicentrarchus labrax}, author = {C C Mylonas and L Anezaki and P Divanach and S Zanuy and F Piferrer and B Ron and A Peduel and I Ben Atia and S Gorshkov and A Tandler}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-20244367191&doi=10.1111%2fj.0022-1112.2005.00766.x&partnerID=40&md5=4bcba3bc5b4f5b44ae84310c6b854a28}, doi = {10.1111/j.0022-1112.2005.00766.x}, issn = {00221112}, year = {2005}, date = {2005-01-01}, journal = {Journal of Fish Biology}, volume = {67}, number = {3}, pages = {652--668}, abstract = {European sea bass Dicentrarchus labrax of the north-western (NW) and south-eastern (SE) Mediterranean Sea strains were exposed to different temperatures (13, 17 or 21°C) during the larval rearing (11-51 days post hatching, dph) or nursery periods (55-95 dph), in order to examine the effects of temperature on sex differentiation and subsequent growth during the first year of life. Higher growth was observed during exposure to higher temperatures, but fish of the NW strain exposed to 13 or 17°C during larval rearing exhibited compensatory growth once exposure to the lower temperatures finished, and as a result their final size at 300 dph was similar or greater to the group exposed to 21°C. Fish exposed to 17°C during the nursery period also had similar size to fish exposed to 21°C after 300 days of rearing, but the fish exposed to 13°C remained significantly smaller (ANOVA}, keywords = {}, pubstate = {published}, tppubtype = {article} } European sea bass Dicentrarchus labrax of the north-western (NW) and south-eastern (SE) Mediterranean Sea strains were exposed to different temperatures (13, 17 or 21°C) during the larval rearing (11-51 days post hatching, dph) or nursery periods (55-95 dph), in order to examine the effects of temperature on sex differentiation and subsequent growth during the first year of life. Higher growth was observed during exposure to higher temperatures, but fish of the NW strain exposed to 13 or 17°C during larval rearing exhibited compensatory growth once exposure to the lower temperatures finished, and as a result their final size at 300 dph was similar or greater to the group exposed to 21°C. Fish exposed to 17°C during the nursery period also had similar size to fish exposed to 21°C after 300 days of rearing, but the fish exposed to 13°C remained significantly smaller (ANOVA |
Poulakakis, N; Goulielmos, G; Antoniou, A; Zouros, E; Mylonas, M Isolation and characterization of polymorphic microsatellite markers in the wall lizard Podarcis erhardii (Squamata: Lacertidae) Journal Article Molecular Ecology Notes, 5 (3), pp. 549–551, 2005. @article{poulakakis_isolation_2005, title = {Isolation and characterization of polymorphic microsatellite markers in the wall lizard Podarcis erhardii (Squamata: Lacertidae)}, author = {N Poulakakis and G Goulielmos and A Antoniou and E Zouros and M Mylonas}, doi = {10.1111/j.1471-8286.2005.00986.x}, year = {2005}, date = {2005-01-01}, journal = {Molecular Ecology Notes}, volume = {5}, number = {3}, pages = {549--551}, abstract = {Five polymorphic microsatellite loci (containing di- and penta-nucleotide repeats) were developed for the Erhard's wall lizard, Podarcis erhardii, an endemic species of southern Balkan Peninsula. The number of alleles ranged from five to 17. Observed and expected heterozygosities ranged from 0.28 to 0.92 and 0.57 to 0.91, respectively. These markers will provide a valuable tool for population genetics analyses and can contribute to the evaluation of conservation programs. © 2005 Blackwell Publishing Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Five polymorphic microsatellite loci (containing di- and penta-nucleotide repeats) were developed for the Erhard's wall lizard, Podarcis erhardii, an endemic species of southern Balkan Peninsula. The number of alleles ranged from five to 17. Observed and expected heterozygosities ranged from 0.28 to 0.92 and 0.57 to 0.91, respectively. These markers will provide a valuable tool for population genetics analyses and can contribute to the evaluation of conservation programs. © 2005 Blackwell Publishing Ltd. |
Mantziou, G; Antoniou, A; Poulakakis, N; Goulielmos, G; Tsigenopoulos, C S; Pinou, T; Mylonas, M Isolation and characterization of six polymorphic microsatellite markers in the freshwater turtle Mauremys rivulata (Testudines: Geoemydidae) Journal Article Molecular Ecology Notes, 5 (4), pp. 727–729, 2005. @article{mantziou_isolation_2005, title = {Isolation and characterization of six polymorphic microsatellite markers in the freshwater turtle Mauremys rivulata (Testudines: Geoemydidae)}, author = {G Mantziou and A Antoniou and N Poulakakis and G Goulielmos and C S Tsigenopoulos and T Pinou and M Mylonas}, url = {http://www.blackwell-synergy.com/doi/abs/10.1111/j.1471-8286.2005.01046.x}, doi = {10.1111/j.1471-8286.2005.01046.x}, year = {2005}, date = {2005-01-01}, journal = {Molecular Ecology Notes}, volume = {5}, number = {4}, pages = {727--729}, abstract = {Abstract Six polymorphic microsatellite loci containing dinucleotide repeats were developed for the freshwater turtle Mauremys rivulata. The number of alleles ranged from five to 18. The observed and expected heterozygosities ranged from 0.19 to 0.79 and 0.46 to 0.90, respectively. These markers may serve as a valuable tool for population genetics analyses and provide information on the evolutionary history of the species.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Six polymorphic microsatellite loci containing dinucleotide repeats were developed for the freshwater turtle Mauremys rivulata. The number of alleles ranged from five to 18. The observed and expected heterozygosities ranged from 0.19 to 0.79 and 0.46 to 0.90, respectively. These markers may serve as a valuable tool for population genetics analyses and provide information on the evolutionary history of the species. |
Xylouri, E; Kotzamanis, Υ P; Athanassopoulou, F; Dong, L; Argyrokastritis, A; Frangiadaki, I Isolation, characterization and sequence determination of Noda virus from sea bass (Dicentrarchus labrax L.) reared in freshwater and marine facilities in Greece Journal Article Bulletin of the Hellenic Veterinary Medical Society, 56 (2), pp. 105–113, 2005. @article{xylouri_isolation_2005, title = {Isolation, characterization and sequence determination of Noda virus from sea bass (Dicentrarchus labrax L.) reared in freshwater and marine facilities in Greece}, author = {E Xylouri and Υ P Kotzamanis and F Athanassopoulou and L Dong and A Argyrokastritis and I Frangiadaki}, year = {2005}, date = {2005-01-01}, journal = {Bulletin of the Hellenic Veterinary Medical Society}, volume = {56}, number = {2}, pages = {105--113}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Mandalakis, M; Apostolaki, M; Stephanou, E G; Stavrakakis, S Mass budget and dynamics of polychlorinated biphenyls in the eastern Mediterranean Sea Journal Article Global Biogeochemical Cycles, 19 (3), pp. 1–16, 2005, ISSN: 08866236. @article{mandalakis_mass_2005, title = {Mass budget and dynamics of polychlorinated biphenyls in the eastern Mediterranean Sea}, author = {M Mandalakis and M Apostolaki and E G Stephanou and S Stavrakakis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-27744457044&doi=10.1029%2f2004GB002403&partnerID=40&md5=c448a5c9bc9bcb79e6dc5fc720676fec}, doi = {10.1029/2004GB002403}, issn = {08866236}, year = {2005}, date = {2005-01-01}, journal = {Global Biogeochemical Cycles}, volume = {19}, number = {3}, pages = {1--16}, abstract = {Polychlorinated biphenyls (PCBs) were measured in dry deposition and sediment trap samples from the eastern Mediterranean and the results of the present and previous studies were synthesized in order to construct a mass balance budget describing the status and dynamics of PCBs in this region. According to our calculations, the burden of total PCBs in the euphotic zone of the eastern Mediterranean should approach 84,000 kg, while the corresponding pool in the overlying atmosphere should be about 162 kg. The atmospheric input of PCBs in the respective water basin through dry and wet deposition should be 300 and 1300 kg yr-1, respectively. On the contrary, air-sea exchange should cause a net volatilization of PCBs from seawater to the atmosphere by 3150 kg yr-1. Moreover, PCBs are removed from the water column with a settling flux of 350 kg yr-1 and under steady state conditions, an additional input of these pollutants should be required to counterbalance the outflows from the water basin. The discharge of urban and industrial sewage, riverine input and transport of more polluted surface waters from western to eastern Mediterranean may account for most of this inflow. In the atmospheric compartment, the removal flux of PCBs due to their destruction by hydroxyl radicals (6650 kg yr-1) was approximately 4 times higher than their deposition flux (wet plus dry). On the basis of these data, 5100 kg of PCBs should enter into the atmosphere of eastern Mediterranean annually in order to achieve steady state conditions. This influx may result from long or short range transport of atmospheric PCBs emitted from contaminated terrestrial surfaces. Copyright 2005 by the American Geophysical Union.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Polychlorinated biphenyls (PCBs) were measured in dry deposition and sediment trap samples from the eastern Mediterranean and the results of the present and previous studies were synthesized in order to construct a mass balance budget describing the status and dynamics of PCBs in this region. According to our calculations, the burden of total PCBs in the euphotic zone of the eastern Mediterranean should approach 84,000 kg, while the corresponding pool in the overlying atmosphere should be about 162 kg. The atmospheric input of PCBs in the respective water basin through dry and wet deposition should be 300 and 1300 kg yr-1, respectively. On the contrary, air-sea exchange should cause a net volatilization of PCBs from seawater to the atmosphere by 3150 kg yr-1. Moreover, PCBs are removed from the water column with a settling flux of 350 kg yr-1 and under steady state conditions, an additional input of these pollutants should be required to counterbalance the outflows from the water basin. The discharge of urban and industrial sewage, riverine input and transport of more polluted surface waters from western to eastern Mediterranean may account for most of this inflow. In the atmospheric compartment, the removal flux of PCBs due to their destruction by hydroxyl radicals (6650 kg yr-1) was approximately 4 times higher than their deposition flux (wet plus dry). On the basis of these data, 5100 kg of PCBs should enter into the atmosphere of eastern Mediterranean annually in order to achieve steady state conditions. This influx may result from long or short range transport of atmospheric PCBs emitted from contaminated terrestrial surfaces. Copyright 2005 by the American Geophysical Union. |
Arvanitidis, C; Atzigeorgiou, G; Koutsoubas, D; Dounas, C; Eleftheriou, A; Koulouri, P Mediterranean lagoons revisited: Weakness and efficiency of the rapid biodiversity assessment techniques in a severely fluctuating environment Journal Article Biodiversity and Conservation, 14 (10), pp. 2347–2359, 2005, ISSN: 09603115. @article{arvanitidis_mediterranean_2005, title = {Mediterranean lagoons revisited: Weakness and efficiency of the rapid biodiversity assessment techniques in a severely fluctuating environment}, author = {C Arvanitidis and G Atzigeorgiou and D Koutsoubas and C Dounas and A Eleftheriou and P Koulouri}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-24944484401&doi=10.1007%2fs10531-004-1668-x&partnerID=40&md5=164959659412afbcf4b012e66162f526}, doi = {10.1007/s10531-004-1668-x}, issn = {09603115}, year = {2005}, date = {2005-01-01}, journal = {Biodiversity and Conservation}, volume = {14}, number = {10}, pages = {2347--2359}, abstract = {The purpose of this paper is to test the performance of rapid biodiversity assessment techniques in the lagoonal environment at the pan-Mediterranean scale. The multivariate techniques can produce patterns of lagoonal biodiversity along the Mediterranean. Additionally, it is shown that the polychaete inventory can preferably be used rather than the aggregation of information at the family level for the purposes of rapid biodiversity assessment. These techniques, however, appear to be weak for the environmental assessment because they cannot detect differences between the naturally disturbed and the anthropogenically impacted lagoons. Both taxonomic distinctness indices are found to be robust in providing meaningful results for rapid biodiversity/environmental assessment when the crustacean inventory and the polychaete and molluscan ones are used for the estimation of the average taxonomic distinctness and of the variation in taxonomic distinctness values, correspondingly. Conversely, information on the distribution of the macrofaunal species to the Mediterranean lagoons appears to be inadequate for the needs of such rapid biodiversity assessment at a regional scale. It is suggested that information on ecological convergence of the macrofaunal species would probably provide biodiversity indices with additional power, at least in the lagoonal environment. © Springer 2005.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The purpose of this paper is to test the performance of rapid biodiversity assessment techniques in the lagoonal environment at the pan-Mediterranean scale. The multivariate techniques can produce patterns of lagoonal biodiversity along the Mediterranean. Additionally, it is shown that the polychaete inventory can preferably be used rather than the aggregation of information at the family level for the purposes of rapid biodiversity assessment. These techniques, however, appear to be weak for the environmental assessment because they cannot detect differences between the naturally disturbed and the anthropogenically impacted lagoons. Both taxonomic distinctness indices are found to be robust in providing meaningful results for rapid biodiversity/environmental assessment when the crustacean inventory and the polychaete and molluscan ones are used for the estimation of the average taxonomic distinctness and of the variation in taxonomic distinctness values, correspondingly. Conversely, information on the distribution of the macrofaunal species to the Mediterranean lagoons appears to be inadequate for the needs of such rapid biodiversity assessment at a regional scale. It is suggested that information on ecological convergence of the macrofaunal species would probably provide biodiversity indices with additional power, at least in the lagoonal environment. © Springer 2005. |
Lika, K; Papandroulakis, N Modeling feeding processes: A test of a new model for sea bream (Sparus aurata) larvae Journal Article Canadian Journal of Fisheries and Aquatic Sciences, 62 , pp. 425 – 435, 2005. @article{lika_modeling_2005, title = {Modeling feeding processes: A test of a new model for sea bream (Sparus aurata) larvae}, author = {K Lika and N Papandroulakis}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-21244489012&partnerID=40&md5=6d09799439dfccab3d0ab5e65d566178}, year = {2005}, date = {2005-01-01}, journal = {Canadian Journal of Fisheries and Aquatic Sciences}, volume = {62}, pages = {425 -- 435}, abstract = {An organism’s feeding rate is governed by constraints imposed by processes associated with consumption. We present a general feeding model that incorporates encounter, successful pursuit, handling, and digestion in one functional representation where we treat digestion as a parallel process. The model produces type II functional response curves. However, the asymptotic maximum feeding rate is determined by the sum of the time spent for handling and digesting a prey minus the gain in time, since the digestion process is parallel to the handling process. We use our model in combination with existing models of encounter, successful pursuit, and digestion to evaluate the feeding rate of fish larvae. We test the model against experimental data for sea bream (Sparus aurata) larvae and find a very close quantitative correspondence between predictions and experiments. Sensitivity analysis shows that for the early developmental stages, the model is sensitive to parameters related to the visual and locomotion abilities of larvae to detect and capture the prey. Later, when they establish these abilities, the choice of accepting or not the prey becomes more important. © 2005 NRC Canada.}, keywords = {}, pubstate = {published}, tppubtype = {article} } An organism’s feeding rate is governed by constraints imposed by processes associated with consumption. We present a general feeding model that incorporates encounter, successful pursuit, handling, and digestion in one functional representation where we treat digestion as a parallel process. The model produces type II functional response curves. However, the asymptotic maximum feeding rate is determined by the sum of the time spent for handling and digesting a prey minus the gain in time, since the digestion process is parallel to the handling process. We use our model in combination with existing models of encounter, successful pursuit, and digestion to evaluate the feeding rate of fish larvae. We test the model against experimental data for sea bream (Sparus aurata) larvae and find a very close quantitative correspondence between predictions and experiments. Sensitivity analysis shows that for the early developmental stages, the model is sensitive to parameters related to the visual and locomotion abilities of larvae to detect and capture the prey. Later, when they establish these abilities, the choice of accepting or not the prey becomes more important. © 2005 NRC Canada. |
Katharios, P; Papadakis, I E; Prapas, A; Dermon, C R; Ampatzis, K; Divanach, P Bulletin of the European Association of Fish Pathologists, 25 (1), pp. 28–31, 2005, ISSN: 01080288. @article{katharios_mortality_2005, title = {Mortality control of viral encephalopathy and retinopathy in 0+ grouper Epinephelus marginatus after prolonged bath in dense Chlorella minutissima culture}, author = {P Katharios and I E Papadakis and A Prapas and C R Dermon and K Ampatzis and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-15744380255&partnerID=40&md5=1882681096115ce168df99dfd0aaf0d8}, issn = {01080288}, year = {2005}, date = {2005-01-01}, journal = {Bulletin of the European Association of Fish Pathologists}, volume = {25}, number = {1}, pages = {28--31}, abstract = {0+ grouper (1±0.2g), presenting symptoms of Viral Encephalopathy and Retinopathy (confirmed by isolation of the virus in SSN-1 cell line and identification by IFAT and DNA sequencing as well as by brain histology), were submitted to seven bath treatment regimes for a 30-day period, using different concentrations of Chlorella minutissima and the antibiotic furaltadone. The cumulative mortalities were 0, 0, 5 and 32% when using 2×108, 108, 5×107 and 2×107 cells of C. minutissima ml-1 respectively, compared to 31-36 % in the control and 55-68% in the antibiotic-treated groups. The beneficial effect, the possible immuno-stimulant and antiviral properties of unicellular micro-algae such as C. minutissima, as well as the role of their concentration in the bath treatment, are discussed.}, keywords = {}, pubstate = {published}, tppubtype = {article} } 0+ grouper (1±0.2g), presenting symptoms of Viral Encephalopathy and Retinopathy (confirmed by isolation of the virus in SSN-1 cell line and identification by IFAT and DNA sequencing as well as by brain histology), were submitted to seven bath treatment regimes for a 30-day period, using different concentrations of Chlorella minutissima and the antibiotic furaltadone. The cumulative mortalities were 0, 0, 5 and 32% when using 2×108, 108, 5×107 and 2×107 cells of C. minutissima ml-1 respectively, compared to 31-36 % in the control and 55-68% in the antibiotic-treated groups. The beneficial effect, the possible immuno-stimulant and antiviral properties of unicellular micro-algae such as C. minutissima, as well as the role of their concentration in the bath treatment, are discussed. |
Gustafsson, Ö; Elmquist, M; Kukulska, Z; Cornelissen, G; Mandalakis, M; Persson, J; Bucheli, T; Reddy, C On the analysis of black carbon in sediments: Evaluation of field data for geochemical consistency Inproceedings Geophysical Research Abstracts, pp. EGU05–A–06089, 2005. @inproceedings{gustafsson_analysis_2005, title = {On the analysis of black carbon in sediments: Evaluation of field data for geochemical consistency}, author = {Ö Gustafsson and M Elmquist and Z Kukulska and G Cornelissen and M Mandalakis and J Persson and T Bucheli and C Reddy}, year = {2005}, date = {2005-01-01}, booktitle = {Geophysical Research Abstracts}, volume = {7}, pages = {EGU05--A--06089}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Koumoundouros, G; Kouttouki, S; Georgakopoulou, E; Papadakis, I; Maingot, E; Kaspiris, P; Kiriakou, Y; Georgiou, G; Divanach, P; Kentouri, M; Mylonas, C C Ontogeny of the shi drum Umbrina cirrosa (Linnaeus 1758), a candidate new species for aquaculture Journal Article Aquaculture Research, 36 (13), pp. 1265–1272, 2005, ISSN: 1355557X. @article{koumoundouros_ontogeny_2005, title = {Ontogeny of the shi drum Umbrina cirrosa (Linnaeus 1758), a candidate new species for aquaculture}, author = {G Koumoundouros and S Kouttouki and E Georgakopoulou and I Papadakis and E Maingot and P Kaspiris and Y Kiriakou and G Georgiou and P Divanach and M Kentouri and C C Mylonas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-25844487755&doi=10.1111%2fj.1365-2109.2005.01314.x&partnerID=40&md5=e26503abd5c1e3f3b24d5805f8231280}, doi = {10.1111/j.1365-2109.2005.01314.x}, issn = {1355557X}, year = {2005}, date = {2005-01-01}, journal = {Aquaculture Research}, volume = {36}, number = {13}, pages = {1265--1272}, abstract = {The ontogeny of shi drum Umbrina cirrosa (Linnaeus 1758), a candidate new species for aquaculture, was studied throughout the entire larval phase. Geometric morphometric analysis revealed two clear inflection points (7.0 and 12.7 mm total length, TL) in the shape ontogeny of this species, separating the studied period into three phases of different allometric priorities. Spline graphs demonstrated that the major non-uniform shape ontogeny correlated with the development of the fins, the anterior dorsal area of the body, the caudal peduncle, the eye and the mouth. Concerning the morphological features, shi drum larvae are characterized by an upward anterior bending of the notochord. The ontogeny of the fins began with the formation of the pectoral buds (2.9 mm TL), continued with the notochord flexion (4.3 mm TL, associated with the caudal fin development), the appearance of the pelvic buds, the first anal rays (4.5 mm TL) and the first dorsal rays (4.8 mm TL). Shi drum juveniles presented 25 vertebrae and the following dominant fin types: D XI,23, AII,6,VI,5, P17 and C17. © 2005 Blackwell Publishing Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The ontogeny of shi drum Umbrina cirrosa (Linnaeus 1758), a candidate new species for aquaculture, was studied throughout the entire larval phase. Geometric morphometric analysis revealed two clear inflection points (7.0 and 12.7 mm total length, TL) in the shape ontogeny of this species, separating the studied period into three phases of different allometric priorities. Spline graphs demonstrated that the major non-uniform shape ontogeny correlated with the development of the fins, the anterior dorsal area of the body, the caudal peduncle, the eye and the mouth. Concerning the morphological features, shi drum larvae are characterized by an upward anterior bending of the notochord. The ontogeny of the fins began with the formation of the pectoral buds (2.9 mm TL), continued with the notochord flexion (4.3 mm TL, associated with the caudal fin development), the appearance of the pelvic buds, the first anal rays (4.5 mm TL) and the first dorsal rays (4.8 mm TL). Shi drum juveniles presented 25 vertebrae and the following dominant fin types: D XI,23, AII,6,VI,5, P17 and C17. © 2005 Blackwell Publishing Ltd. |
Szisch, V; Papandroulakis, N; Fanouraki, E; Pavlidis, M Ontogeny of the thyroid hormones and cortisol in the gilthead sea bream, Sparus aurata Journal Article General and Comparative Endocrinology, 142 , pp. 186 – 192, 2005. @article{szisch_ontogeny_2005, title = {Ontogeny of the thyroid hormones and cortisol in the gilthead sea bream, Sparus aurata}, author = {V Szisch and N Papandroulakis and E Fanouraki and M Pavlidis}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-18144427445&partnerID=40&md5=95ebcdb3f57636df10429eeea10cafa2}, year = {2005}, date = {2005-01-01}, journal = {General and Comparative Endocrinology}, volume = {142}, pages = {186 -- 192}, abstract = {The aim of the study was to elucidate the pattern of changes in the whole body concentrations of thyroid hormones (TH) and cortisol in eggs, pre-larvae, and larvae in the gilthead sea bream, Sparus aurata. The ontogeny of these hormones was related to specific morphological characteristics that characterize early development. TH and cortisol were detected in substantial amounts in the eggs of sea bream. T3 concentration in eggs was found to be higher than that of T4 (T3, 4.02 ± 0.38 ng g-1; T4, 1.63 ± 0.28 ng g-1), as it is often observed in marine teleosts. T4 decreased until hatching (T4, 1.01 ± 0.58 ng g-1), while T3 reached a minimum at the time of first feeding (T3, 0.19 ± 0.02 ng g-1). As development proceeded, both hormones displayed a similar pattern of changes with increasing concentrations until the completion of metamorphosis (T3, 15.84 ± 2.92 ng g-1; T4, 9.78 ± 2.39 ng g-1), indicating that the hypothalamo-adenohypophysial-thyroid axis begins to function soon after first feeding. The pattern of changes of cortisol (F) concentration was almost parallel to that of T4, starting from 0.83 ng g-1 in eggs. Minimum F concentration was observed at hatching (0.39 ± 0.03 ng g-1) and maximum at melanophores and scale formation (14.82 ± 2.71 and 14.82 ± 3.12 ng g-1, respectively). Results provide data for the first time on the ontogeny of thyroid hormones and cortisol during the early development in sea bream and are in agreement with results in other fish species for an important action of TH during early development. © 2005 Elsevier Inc. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The aim of the study was to elucidate the pattern of changes in the whole body concentrations of thyroid hormones (TH) and cortisol in eggs, pre-larvae, and larvae in the gilthead sea bream, Sparus aurata. The ontogeny of these hormones was related to specific morphological characteristics that characterize early development. TH and cortisol were detected in substantial amounts in the eggs of sea bream. T3 concentration in eggs was found to be higher than that of T4 (T3, 4.02 ± 0.38 ng g-1; T4, 1.63 ± 0.28 ng g-1), as it is often observed in marine teleosts. T4 decreased until hatching (T4, 1.01 ± 0.58 ng g-1), while T3 reached a minimum at the time of first feeding (T3, 0.19 ± 0.02 ng g-1). As development proceeded, both hormones displayed a similar pattern of changes with increasing concentrations until the completion of metamorphosis (T3, 15.84 ± 2.92 ng g-1; T4, 9.78 ± 2.39 ng g-1), indicating that the hypothalamo-adenohypophysial-thyroid axis begins to function soon after first feeding. The pattern of changes of cortisol (F) concentration was almost parallel to that of T4, starting from 0.83 ng g-1 in eggs. Minimum F concentration was observed at hatching (0.39 ± 0.03 ng g-1) and maximum at melanophores and scale formation (14.82 ± 2.71 and 14.82 ± 3.12 ng g-1, respectively). Results provide data for the first time on the ontogeny of thyroid hormones and cortisol during the early development in sea bream and are in agreement with results in other fish species for an important action of TH during early development. © 2005 Elsevier Inc. All rights reserved. |
Poulakakis, N; Lymberakis, P; Tsigenopoulos, C S; Magoulas, A; Mylonas, M Phylogenetic relationships and evolutionary history of snake-eyed skink Ablepharus kitaibelii (Sauria: Scincidae) Journal Article Molecular Phylogenetics and Evolution, 34 , pp. 245 – 256, 2005. @article{poulakakis_phylogenetic_2005, title = {Phylogenetic relationships and evolutionary history of snake-eyed skink Ablepharus kitaibelii (Sauria: Scincidae)}, author = {N Poulakakis and P Lymberakis and C S Tsigenopoulos and A Magoulas and M Mylonas}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-11144266691&partnerID=40&md5=9ec5b39f41ecfb4a0f8fdb0d7b315756}, year = {2005}, date = {2005-01-01}, journal = {Molecular Phylogenetics and Evolution}, volume = {34}, pages = {245 -- 256}, abstract = {Sequence data derived from two mitochondrial markers, 16S rRNA and cytochrome b genes, were used to infer the phylogenetic relationships of 38 populations of the snake-eyed skinks of the genus Ablepharus with emphasis on A. kitaibelii from Greece and Turkey. The partition-homogeneity tests indicated that the combined data set was homogeneous, and maximum-parsimony, maximum-likelihood, and Bayesian analyses produced topologically identical trees that revealed a well-resolved phylogeny. All species except A. kitaibelii form monophyletic units. The latter species appears paraphyletic with respect to A. budaki and A. chernovi with populations clustering into two distinct clades. A. chernovi and A. budaki, which have recently been raised to species status, were confirmed as genetically distinct forms. We used sequence divergence and paleogeographic history of the Aegean region to reconstruct a biogeographic evolutionary scenario for A. kitaibelii. © 2004 Elsevier Inc. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Sequence data derived from two mitochondrial markers, 16S rRNA and cytochrome b genes, were used to infer the phylogenetic relationships of 38 populations of the snake-eyed skinks of the genus Ablepharus with emphasis on A. kitaibelii from Greece and Turkey. The partition-homogeneity tests indicated that the combined data set was homogeneous, and maximum-parsimony, maximum-likelihood, and Bayesian analyses produced topologically identical trees that revealed a well-resolved phylogeny. All species except A. kitaibelii form monophyletic units. The latter species appears paraphyletic with respect to A. budaki and A. chernovi with populations clustering into two distinct clades. A. chernovi and A. budaki, which have recently been raised to species status, were confirmed as genetically distinct forms. We used sequence divergence and paleogeographic history of the Aegean region to reconstruct a biogeographic evolutionary scenario for A. kitaibelii. © 2004 Elsevier Inc. All rights reserved. |
Koulouri, P; Dounas, C; Eleftheriou, A Preliminary results on the effect of otter trawling on hyperbenthic communities in Heraklion Bay (Eastern Mediterranean, Cretan Sea) Journal Article American Fisheries Society Symposium, 41 , pp. 529–537, 2005. @article{koulouri_preliminary_2005, title = {Preliminary results on the effect of otter trawling on hyperbenthic communities in Heraklion Bay (Eastern Mediterranean, Cretan Sea)}, author = {P Koulouri and C Dounas and A Eleftheriou}, year = {2005}, date = {2005-01-01}, journal = {American Fisheries Society Symposium}, volume = {41}, pages = {529--537}, abstract = {Although the benthopelagic fish species are a focus of commercial exploitation, relatively little attention has been paid to the small-sized invertebrates (0.5-20mm) living on or very close to the seabed, thus inhabiting the same biotope, known as well as “hyperbenthos”. Recently interest in this faunal group has increased, as many demersal fish and epibenthic crustaceans have been found to feed on hyperbenthic animals, for at least part of their lives. Otter trawls, the most common gear used for demersal fishing, result in significant disturbance of the sediment-water interface. Animals that are disturbed by the passage of a trawl may become more available to predators and scavengers. We have been unable to find any reports of studies of the impacts of towed fishing gears, including otter trawls, on hyperbenthos. We studied these effects on the Mediterranean continental shelf in Heraklion Bay (Cretan Sea), using a novel apparatus to simulate the contact of otter trawl groundrope with the sea bed. A modified three-level hyperbenthic sledge was used for collecting disturbed (groundrope present) and undisturbed (without groundrope) macrofaunal samples at a towing speed typical of the local commercial vessels. Observations were made in an area being actively fished, before and during the trawling season. The preliminary results reported here indicate that trawling causes significant changes in the structure of hyperbenthic communities.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Although the benthopelagic fish species are a focus of commercial exploitation, relatively little attention has been paid to the small-sized invertebrates (0.5-20mm) living on or very close to the seabed, thus inhabiting the same biotope, known as well as “hyperbenthos”. Recently interest in this faunal group has increased, as many demersal fish and epibenthic crustaceans have been found to feed on hyperbenthic animals, for at least part of their lives. Otter trawls, the most common gear used for demersal fishing, result in significant disturbance of the sediment-water interface. Animals that are disturbed by the passage of a trawl may become more available to predators and scavengers. We have been unable to find any reports of studies of the impacts of towed fishing gears, including otter trawls, on hyperbenthos. We studied these effects on the Mediterranean continental shelf in Heraklion Bay (Cretan Sea), using a novel apparatus to simulate the contact of otter trawl groundrope with the sea bed. A modified three-level hyperbenthic sledge was used for collecting disturbed (groundrope present) and undisturbed (without groundrope) macrofaunal samples at a towing speed typical of the local commercial vessels. Observations were made in an area being actively fished, before and during the trawling season. The preliminary results reported here indicate that trawling causes significant changes in the structure of hyperbenthic communities. |
Athanassopoulou, F; Ragias, V; Vagianou, St.; Cave, Di D; Rigos, G; Papathanasiou, G; Georgoulakis, J Bulletin of the European Association of Fish Pathologists, 25 (6), pp. 256–261, 2005, ISSN: 01080288. @article{athanassopoulou_report_2005, title = {Report of Sparicotyle (Microcotyle) chrysophrii Van Beneden and Hesse 1863, Atrispinum seminalis Euzet and Maillard 1973 and Polylabris tubicirrus Paperna and Kohn 1964 (Monogenea) on captive sea bream (Sparus aurata) and sharp snout sea bream (Diplodus puntazzo) in coastal Greece and Italy}, author = {F Athanassopoulou and V Ragias and St. Vagianou and D Di Cave and G Rigos and G Papathanasiou and J Georgoulakis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-32644449166&partnerID=40&md5=2a4af286e79a6d976aab89a00362db06}, issn = {01080288}, year = {2005}, date = {2005-01-01}, journal = {Bulletin of the European Association of Fish Pathologists}, volume = {25}, number = {6}, pages = {256--261}, abstract = {During the years 2000-2003, caged gilthead sea bream (Sparus aurata L.) and sharp snout sea bream (Diplodus puntazzo C.) from two farms in Greece and two farms in Italy (the Central Thyrrenian Sea) were examined. Sparus aurata from Greece was moderately infected with Furnestinia echeneis and Sparicotyle chrysophrii. Diplodus puntazzo from the Greek farms were infected with three polyopisthocotylean monogeneans: Atrispinum salpae, A. seminalis and S. chrysophrii. In Italy, on the gills of cultivated S. aurata, a polyopisthocotylean parasite was identified as Polylabris tubicirrus which is considered host specific and only affecting breams of the genus Diplodus. Furthermore, D. puntazzo and S. aurata showed the transfer of the monogenean S. chrysophrii from S. aurata (its type host) to D. puntazzo which is never found infected in wild conditions by this parasite. The presence of these parasites, and in particular S. chrysophrii in cultured D. puntazzo, is an important finding for aquaculturists in terms of potential disease problems.}, keywords = {}, pubstate = {published}, tppubtype = {article} } During the years 2000-2003, caged gilthead sea bream (Sparus aurata L.) and sharp snout sea bream (Diplodus puntazzo C.) from two farms in Greece and two farms in Italy (the Central Thyrrenian Sea) were examined. Sparus aurata from Greece was moderately infected with Furnestinia echeneis and Sparicotyle chrysophrii. Diplodus puntazzo from the Greek farms were infected with three polyopisthocotylean monogeneans: Atrispinum salpae, A. seminalis and S. chrysophrii. In Italy, on the gills of cultivated S. aurata, a polyopisthocotylean parasite was identified as Polylabris tubicirrus which is considered host specific and only affecting breams of the genus Diplodus. Furthermore, D. puntazzo and S. aurata showed the transfer of the monogenean S. chrysophrii from S. aurata (its type host) to D. puntazzo which is never found infected in wild conditions by this parasite. The presence of these parasites, and in particular S. chrysophrii in cultured D. puntazzo, is an important finding for aquaculturists in terms of potential disease problems. |
Polymenakou, P N; Tselepides, A; Stephanou, E G Continental Shelf Research, 25 , pp. 2196–2212, 2005. @article{polymenakou_study_2005, title = {Study of the mineralization effect on the distribution of lipids in sediments from the Cretan Sea: Evidence for hydrocarbon degradation and starvation stress}, author = {P N Polymenakou and A Tselepides and E G Stephanou}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-27744476221&partnerID=40&md5=d20fdba5930d83604c3f1010a58783da}, year = {2005}, date = {2005-01-01}, journal = {Continental Shelf Research}, volume = {25}, pages = {2196--2212}, abstract = {Sedimentary diagenetic processes alter the composition and distribution of different lipid compounds. In the present study alterations mediated by microbial communities were investigated along a bathymetric gradient (100 m at 35°23'N-25°09'E, 617 m at 35°33'N- 25°08'E, 1494 m at 35°44'N-25°08'E) over the continental margin of northern Crete (Greece, Eastern Mediterranean Sea). Bacterial abundances and distribution were studied using phospholipid linked fatty acids (PLFA), in the range of C8-C22, released from intact phospholipids. Lipid components (aliphatic hydrocarbons, free fatty acids, glycerides and glycolipids) were studied over a 2-month incubation period. Carbon mineralization rates at all stations indicated an uneven distribution of active aerobic bacteria with values decreasing towards the deeper stations. PLFA homologue profiles denoted that aerobic gram negative and sulfur oxidizing bacteria dominated microbial communities while the anaerobic, gram positive and sulfate reducing bacteria occurred only in traces. The n-alkane (NA) composition revealed a strong predominance of homologues with odd carbon numbers suggesting an important terrestrial contribution to the sediments. The estimated descriptive ratios of NA, the sum of short chain NA (C15-C20) and long chain NA (C21-C 36) to 17α(H),21β(H)-C30-hopane, before and after a two-month incubation period, indicated the occurrence of hydrocarbon degradation processes. Increased ratios of saturated to unsaturated fatty acids were also recorded after the incubation indicating the starvation of bacterial communities by the end of the experiments. © 2005 Elsevier Ltd. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Sedimentary diagenetic processes alter the composition and distribution of different lipid compounds. In the present study alterations mediated by microbial communities were investigated along a bathymetric gradient (100 m at 35°23'N-25°09'E, 617 m at 35°33'N- 25°08'E, 1494 m at 35°44'N-25°08'E) over the continental margin of northern Crete (Greece, Eastern Mediterranean Sea). Bacterial abundances and distribution were studied using phospholipid linked fatty acids (PLFA), in the range of C8-C22, released from intact phospholipids. Lipid components (aliphatic hydrocarbons, free fatty acids, glycerides and glycolipids) were studied over a 2-month incubation period. Carbon mineralization rates at all stations indicated an uneven distribution of active aerobic bacteria with values decreasing towards the deeper stations. PLFA homologue profiles denoted that aerobic gram negative and sulfur oxidizing bacteria dominated microbial communities while the anaerobic, gram positive and sulfate reducing bacteria occurred only in traces. The n-alkane (NA) composition revealed a strong predominance of homologues with odd carbon numbers suggesting an important terrestrial contribution to the sediments. The estimated descriptive ratios of NA, the sum of short chain NA (C15-C20) and long chain NA (C21-C 36) to 17α(H),21β(H)-C30-hopane, before and after a two-month incubation period, indicated the occurrence of hydrocarbon degradation processes. Increased ratios of saturated to unsaturated fatty acids were also recorded after the incubation indicating the starvation of bacterial communities by the end of the experiments. © 2005 Elsevier Ltd. All rights reserved. |
Manoudis, G; Antoniadou, Chryssanthi; Dounas, C; Chintiroglou, Chariton Charles Successional stages of experimental artificial reefs deployed in Vistonikos gulf (North SAegean Sea, Greece): Preliminary results Journal Article Belgian Journal of Zoology, 135 , pp. 209–215, 2005. @article{manoudis_successional_2005, title = {Successional stages of experimental artificial reefs deployed in Vistonikos gulf (North SAegean Sea, Greece): Preliminary results}, author = {G Manoudis and Chryssanthi Antoniadou and C Dounas and Chariton Charles Chintiroglou}, year = {2005}, date = {2005-01-01}, journal = {Belgian Journal of Zoology}, volume = {135}, pages = {209--215}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Koutandos, E; Prinos, P Supercritical free-surface turbulent flow over a semicircular obstacle Inproceedings pp. 1366–1376, 2005, (Publication Title: XXXI IAHR Congress). @inproceedings{koutandos_supercritical_2005, title = {Supercritical free-surface turbulent flow over a semicircular obstacle}, author = {E Koutandos and P Prinos}, year = {2005}, date = {2005-01-01}, volume = {1}, pages = {1366--1376}, note = {Publication Title: XXXI IAHR Congress}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Chatzifotis, S; Pavlidis, M; Jimeno, C D; Vardanis, G; Sterioti, A; Divanach, P The effect of different carotenoid sources on skin coloration of cultured red porgy (Pagrus pagrus) Journal Article Aquaculture Research, 36 (15), pp. 1517–1525, 2005, ISSN: 1355557X. @article{chatzifotis_effect_2005-1, title = {The effect of different carotenoid sources on skin coloration of cultured red porgy (Pagrus pagrus)}, author = {S Chatzifotis and M Pavlidis and C D Jimeno and G Vardanis and A Sterioti and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-28144454613&doi=10.1111%2fj.1365-2109.2005.01374.x&partnerID=40&md5=70151f9b5623aeec3809a55ae9d2a95d}, doi = {10.1111/j.1365-2109.2005.01374.x}, issn = {1355557X}, year = {2005}, date = {2005-01-01}, journal = {Aquaculture Research}, volume = {36}, number = {15}, pages = {1517--1525}, abstract = {This study presents data on the effect of carotenoid sources on skin coloration of red porgy (Pagrus pagrus). Three experiments were conducted: in the first, fish were fed an astaxanthin (Naturose®)-supplemented diet, while the second fish received diets supplemented with β-carotene (Rovimix β-caroten®) or lycopene (Lyc-O-Mato®): Carotenoids were added to the level of 100 ppm in each diet, while a non-carotenoid-supplemented diet served as a control. In the third experiment, the effect of dietary protein/carbohydrate ratio on melanin content in the skin was investigated. For this experimentation, four diets were formulated to contain 50/23, 40/32, 30/48 and 20/59 protein/carbohydrate ratio. Naturose astaxanthin increased total carotenoid content in the dorsal skin area while β-carotene and lycopene seem to have had no significant effect. Naturose was the only carotenoid source that had a significant effect on skin hue, promoting a reddish coloration to the dorsal skin area and a ventral hue similar to wild red porgy. No apparent effect of carotenoid source on skin melanin content was observed. In contrast, dietary protein/ carbohydrate ratio affected melanin content in the skin. The fish fed the 50/23 diet showed significantly higher values. Farmed red porgy had eight times higher dorsal-skin melanin content than wild ones. © 2005 Blackwell Publishing Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study presents data on the effect of carotenoid sources on skin coloration of red porgy (Pagrus pagrus). Three experiments were conducted: in the first, fish were fed an astaxanthin (Naturose®)-supplemented diet, while the second fish received diets supplemented with β-carotene (Rovimix β-caroten®) or lycopene (Lyc-O-Mato®): Carotenoids were added to the level of 100 ppm in each diet, while a non-carotenoid-supplemented diet served as a control. In the third experiment, the effect of dietary protein/carbohydrate ratio on melanin content in the skin was investigated. For this experimentation, four diets were formulated to contain 50/23, 40/32, 30/48 and 20/59 protein/carbohydrate ratio. Naturose astaxanthin increased total carotenoid content in the dorsal skin area while β-carotene and lycopene seem to have had no significant effect. Naturose was the only carotenoid source that had a significant effect on skin hue, promoting a reddish coloration to the dorsal skin area and a ventral hue similar to wild red porgy. No apparent effect of carotenoid source on skin melanin content was observed. In contrast, dietary protein/ carbohydrate ratio affected melanin content in the skin. The fish fed the 50/23 diet showed significantly higher values. Farmed red porgy had eight times higher dorsal-skin melanin content than wild ones. © 2005 Blackwell Publishing Ltd. |
Dounas, C; Davies, I; Hayes, P; Arvanitidis, C; Koulouri, P The effect of different types of otter trawl groundrope on benthic nutrient fluxes and sediment biogeochemistry Journal Article American Fisheries Society Symposium, 41 , pp. 539–544, 2005. @article{dounas_effect_2005, title = {The effect of different types of otter trawl groundrope on benthic nutrient fluxes and sediment biogeochemistry}, author = {C Dounas and I Davies and P Hayes and C Arvanitidis and P Koulouri}, url = {http://fisheries.org/shop/x54041xm}, year = {2005}, date = {2005-01-01}, journal = {American Fisheries Society Symposium}, volume = {41}, pages = {539--544}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Kasapidis, P; Magoulas, A; Mylonas, M; Zouros, E The phylogeography of the gecko Cyrtopodion kotschyi (Reptilia: Gekkonidae) in the Aegean archipelago Journal Article Molecular Phylogenetics and Evolution, 35 (3), pp. 612–623, 2005, ISSN: 10557903. @article{kasapidis_phylogeography_2005, title = {The phylogeography of the gecko Cyrtopodion kotschyi (Reptilia: Gekkonidae) in the Aegean archipelago}, author = {P Kasapidis and A Magoulas and M Mylonas and E Zouros}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-18144425199&doi=10.1016%2fj.ympev.2005.02.005&partnerID=40&md5=51a9fa8e77d60205ccf8124439085449}, doi = {10.1016/j.ympev.2005.02.005}, issn = {10557903}, year = {2005}, date = {2005-01-01}, journal = {Molecular Phylogenetics and Evolution}, volume = {35}, number = {3}, pages = {612--623}, abstract = {Cyrtopodion kotschyi is a small gecko, widely distributed on the islands of the Aegean archipelago and the adjacent mainland. We unveiled the phylogeography of the species by using a portion of the cytochrome oxidase I mitochondrial DNA gene from 35 insular and mainland populations. The distinct geographic distribution of the major clades of the phylogenetic tree and its topology suggest a spatial and temporal sequence of phylogenetic separations that coincide with some major paleogeographical separations during the geological history of the Aegean and support a mainly vicariant pattern of differentiation. The separation times and 95% confidence limits among the different clades were estimated according to two different paleogeographical scenarios. The very high interpopulation genetic divergence (up to 20% uncorrected pairwise distances) and the better concordance between paleogeographical and phylogenetic separations for one of the scenarios suggest that species differentiation started during Miocene (about 10 Mya) due to the fragmentation of the united landmass of "Ägäis" that was Aegean at that time. © 2005 Elsevier Inc. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Cyrtopodion kotschyi is a small gecko, widely distributed on the islands of the Aegean archipelago and the adjacent mainland. We unveiled the phylogeography of the species by using a portion of the cytochrome oxidase I mitochondrial DNA gene from 35 insular and mainland populations. The distinct geographic distribution of the major clades of the phylogenetic tree and its topology suggest a spatial and temporal sequence of phylogenetic separations that coincide with some major paleogeographical separations during the geological history of the Aegean and support a mainly vicariant pattern of differentiation. The separation times and 95% confidence limits among the different clades were estimated according to two different paleogeographical scenarios. The very high interpopulation genetic divergence (up to 20% uncorrected pairwise distances) and the better concordance between paleogeographical and phylogenetic separations for one of the scenarios suggest that species differentiation started during Miocene (about 10 Mya) due to the fragmentation of the united landmass of "Ägäis" that was Aegean at that time. © 2005 Elsevier Inc. All rights reserved. |
Kasapidis, P; Suchentrunk, F; Magoulas, A; Kotoulas, G Molecular Phylogenetics and Evolution, 34 (1), pp. 55–66, 2005, ISSN: 10557903. @article{kasapidis_shaping_2005, title = {The shaping of mitochondrial DNA phylogeographic patterns of the brown hare (Lepus europaeus) under the combined influence of Late Pleistocene climatic fluctuations and anthropogenic translocations}, author = {P Kasapidis and F Suchentrunk and A Magoulas and G Kotoulas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-9744258860&doi=10.1016%2fj.ympev.2004.09.007&partnerID=40&md5=a65c976ffbb7419b3edd624a3efbeb4d}, doi = {10.1016/j.ympev.2004.09.007}, issn = {10557903}, year = {2005}, date = {2005-01-01}, journal = {Molecular Phylogenetics and Evolution}, volume = {34}, number = {1}, pages = {55--66}, abstract = {The phylogeographic structure of the brown hare (Lepus europaeus) was studied by analysing mtDNA control region sequences of 98 individuals from continental and insular Greece, Bulgaria, Cyprus and northern Israel, together with 44 published sequences from Italy and central Europe. We found two distinct clades separated by an average nucleotide divergence of 6.6%, which may correspond to a Balkan and to an Asia Minor refugium. The estimated time of separation of the two clades was dated back to 105,000- 490,000 years ago. These two clades coexist in the area of northeastern Greece and Bulgaria, most likely as a result of a post-glacial northward expansion. Within the southern Balkan refugium, network analyses showed geographical structuring, which supports the hypothesis of several isolated Late Pleistocene populations. The central European and Italian populations appear to have originated from a non-detected northern Balkan population that was genetically closely related to some northern Greek populations, as a result of postglacial expansion, translocations or a combination of both. Moreover, several cases of ancient and recent translocations by humans were detected, especially for some island populations, while the eastern Aegean islands off the Asia Minor coast were most likely colonized naturally through Late Pleistocene land bridge connection. The genetic analysis presented here provides a framework for designing proper conservation and management guidelines for this species. © 2004 Elsevier Inc. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The phylogeographic structure of the brown hare (Lepus europaeus) was studied by analysing mtDNA control region sequences of 98 individuals from continental and insular Greece, Bulgaria, Cyprus and northern Israel, together with 44 published sequences from Italy and central Europe. We found two distinct clades separated by an average nucleotide divergence of 6.6%, which may correspond to a Balkan and to an Asia Minor refugium. The estimated time of separation of the two clades was dated back to 105,000- 490,000 years ago. These two clades coexist in the area of northeastern Greece and Bulgaria, most likely as a result of a post-glacial northward expansion. Within the southern Balkan refugium, network analyses showed geographical structuring, which supports the hypothesis of several isolated Late Pleistocene populations. The central European and Italian populations appear to have originated from a non-detected northern Balkan population that was genetically closely related to some northern Greek populations, as a result of postglacial expansion, translocations or a combination of both. Moreover, several cases of ancient and recent translocations by humans were detected, especially for some island populations, while the eastern Aegean islands off the Asia Minor coast were most likely colonized naturally through Late Pleistocene land bridge connection. The genetic analysis presented here provides a framework for designing proper conservation and management guidelines for this species. © 2004 Elsevier Inc. All rights reserved. |
Katharios, P; Smullen, R P; Inglis, V The use of the polychaete worm Nereis virens eggs as vehicle for the delivery of oxytetracycline in Solea solea larvae Journal Article Aquaculture, 243 (1-4), pp. 1–7, 2005, ISSN: 00448486. @article{katharios_use_2005, title = {The use of the polychaete worm Nereis virens eggs as vehicle for the delivery of oxytetracycline in Solea solea larvae}, author = {P Katharios and R P Smullen and V Inglis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-10644230309&doi=10.1016%2fj.aquaculture.2004.09.031&partnerID=40&md5=cd90fc4dea25c5202c986ac6b4710655}, doi = {10.1016/j.aquaculture.2004.09.031}, issn = {00448486}, year = {2005}, date = {2005-01-01}, journal = {Aquaculture}, volume = {243}, number = {1-4}, pages = {1--7}, abstract = {The eggs of the polychaete worm Nereis virens were used as a vehicle for the delivery of oxytetracycline (OTC) in Dover sole, Solea solea larvae. The eggs were osmotically loaded, and the level of antibiotic was estimated by HPLC and a microbiological bioassay. The amount of OTC in the N. virens eggs was over 1 μg per egg, which was considered more than sufficient for a therapeutic dose for larval fish. The loaded eggs were tested on the sole larvae, and the results of the experiment (9 μg OTC per fish larva) indicated that the eggs of the Nereis worm can be used as biocarriers of water soluble drugs. © 2004 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The eggs of the polychaete worm Nereis virens were used as a vehicle for the delivery of oxytetracycline (OTC) in Dover sole, Solea solea larvae. The eggs were osmotically loaded, and the level of antibiotic was estimated by HPLC and a microbiological bioassay. The amount of OTC in the N. virens eggs was over 1 μg per egg, which was considered more than sufficient for a therapeutic dose for larval fish. The loaded eggs were tested on the sole larvae, and the results of the experiment (9 μg OTC per fish larva) indicated that the eggs of the Nereis worm can be used as biocarriers of water soluble drugs. © 2004 Elsevier B.V. All rights reserved. |
2004 |
Grigorakis, Kriton; Alexis, Maria; Gialamas, Ioannis; Nikolopoulou, Demetra Sensory, microbiological, and chemical spoilage of cultured common sea bass ( Dicentrarchus labrax) stored in ice: a seasonal differentiation Journal Article European Food Research and Technology, 219 (6), pp. 584–587, 2004, ISSN: 1438-2377, 1438-2385. @article{grigorakis_sensory_2004, title = {Sensory, microbiological, and chemical spoilage of cultured common sea bass ( Dicentrarchus labrax) stored in ice: a seasonal differentiation}, author = {Kriton Grigorakis and Maria Alexis and Ioannis Gialamas and Demetra Nikolopoulou}, url = {http://link.springer.com/10.1007/s00217-004-1005-3}, doi = {10.1007/s00217-004-1005-3}, issn = {1438-2377, 1438-2385}, year = {2004}, date = {2004-11-01}, urldate = {2020-08-10}, journal = {European Food Research and Technology}, volume = {219}, number = {6}, pages = {584--587}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Marcouli, P A; Alexis, M N; Andriopoulou, A; Iliopoulou-Georgudaki, J Development of a reference diet for use in indispensable amino acid requirement studies of gilthead seabream Sparus aurata L. Journal Article Aquaculture Nutrition, 10 (5), pp. 335–343, 2004, ISSN: 1353-5773, 1365-2095. @article{marcouli_development_2004, title = {Development of a reference diet for use in indispensable amino acid requirement studies of gilthead seabream Sparus aurata L.}, author = {P A Marcouli and M N Alexis and A Andriopoulou and J Iliopoulou-Georgudaki}, url = {http://doi.wiley.com/10.1111/j.1365-2095.2004.00308.x}, doi = {10.1111/j.1365-2095.2004.00308.x}, issn = {1353-5773, 1365-2095}, year = {2004}, date = {2004-10-01}, urldate = {2020-08-17}, journal = {Aquaculture Nutrition}, volume = {10}, number = {5}, pages = {335--343}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
P., Katharios; I.E., Papadakis; A., Prapas; C.R., Dermon; K., Ampatzis; Divanach, P Mortality control of VNN disease in 0+ grouper, Epinephelus marginatus after prolonged bath in dense Chlorella minutissima culture. Inproceedings EAS, (Ed.): European Aquaculture Society, Biotechnologies for Quality, Barcelona, Spain, 2004. @inproceedings{katharios_p_mortality_2004, title = {Mortality control of VNN disease in 0+ grouper, Epinephelus marginatus after prolonged bath in dense Chlorella minutissima culture.}, author = {Katharios P. and Papadakis I.E. and Prapas A. and Dermon C.R. and Ampatzis K. and P Divanach}, editor = {EAS}, year = {2004}, date = {2004-10-01}, booktitle = {European Aquaculture Society, Biotechnologies for Quality}, address = {Barcelona, Spain}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Koumoundouros, G; Carrillo, J; Divanach, P; Kentouri, M The rearing of common dentex Dentex dentex (L.) During the hatchery and on-growing phases Journal Article Aquaculture, 240 (1-4), pp. 165–173, 2004, ISSN: 00448486. @article{koumoundouros_rearing_2004, title = {The rearing of common dentex Dentex dentex (L.) During the hatchery and on-growing phases}, author = {G Koumoundouros and J Carrillo and P Divanach and M Kentouri}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848604000869}, doi = {10.1016/j.aquaculture.2004.01.038}, issn = {00448486}, year = {2004}, date = {2004-10-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {240}, number = {1-4}, pages = {165--173}, abstract = {In the current paper we present the rearing methodology of common dentex (Dentex dentex), a promising candidate for Mediterranean marine aquaculture, from the larval to the on-growing phase. The results are discussed in terms of the efficiency of rearing methodology followed, for the mass production of the species. © 2004 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In the current paper we present the rearing methodology of common dentex (Dentex dentex), a promising candidate for Mediterranean marine aquaculture, from the larval to the on-growing phase. The results are discussed in terms of the efficiency of rearing methodology followed, for the mass production of the species. © 2004 Elsevier B.V. All rights reserved. |
Koutandos, E V; Karambas, Th. V; Koutitas, C G Floating Breakwater Response to Waves Action Using a Boussinesq Model Coupled with a 2DV Elliptic Solver Journal Article Journal of Waterway, Port, Coastal, and Ocean Engineering, 130 (5), pp. 243–255, 2004, ISSN: 0733-950X, 1943-5460. @article{koutandos_floating_2004, title = {Floating Breakwater Response to Waves Action Using a Boussinesq Model Coupled with a 2DV Elliptic Solver}, author = {E V Koutandos and Th. V Karambas and C G Koutitas}, url = {http://ascelibrary.org/doi/10.1061/%28ASCE%290733-950X%282004%29130%3A5%28243%29}, doi = {10.1061/(ASCE)0733-950X(2004)130:5(243)}, issn = {0733-950X, 1943-5460}, year = {2004}, date = {2004-09-01}, urldate = {2020-08-18}, journal = {Journal of Waterway, Port, Coastal, and Ocean Engineering}, volume = {130}, number = {5}, pages = {243--255}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Kasapidis, P; Planes, S; Laurent, V; Quinta, R; Teia, A; Lenfant, R; Darivianakis, S; Terzoglou, V; Kotoulas, G; Magoulas, A Stock discrimination and temporal and spatial genetic variation of sardine (Sardina pilchardus) in northeastern Atlantic, with a combined analysis of nuclear (microsatellites and allozymes) and mitochondrial DNA markers. Inproceedings Kasapidis, P (Ed.): 2004 ICES Annual Science Conference, 2004. @inproceedings{kasapidis_stock_2004, title = {Stock discrimination and temporal and spatial genetic variation of sardine (Sardina pilchardus) in northeastern Atlantic, with a combined analysis of nuclear (microsatellites and allozymes) and mitochondrial DNA markers.}, author = {P Kasapidis and S Planes and V Laurent and R Quinta and A Teia and R Lenfant and S Darivianakis and V Terzoglou and G Kotoulas and A Magoulas}, editor = {P Kasapidis}, year = {2004}, date = {2004-09-01}, booktitle = {2004 ICES Annual Science Conference}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Publications
2005 |
Evaluation of candidate probiotic strains for gilthead sea bream larvae (Sparus aurata) using an in vivo approach Journal Article Letters in Applied Microbiology, 40 (4), pp. 274–277, 2005, ISSN: 0266-8254, 1365-2673. |
Links between Geographic Location, Environmental Factors, and Microbial Community Composition in Sediments of the Eastern Mediterranean Sea Journal Article Microbial Ecology, 49 (3), pp. 367–378, 2005, ISSN: 0095-3628, 1432-184X. |
Transposable element polymorphism of Wolbachia in the mosquito Culex pipiens: evidence of genetic diversity, superinfection and recombination Journal Article Molecular Ecology, 14 (5), pp. 1561–1573, 2005, ISSN: 0962-1083, 1365-294X. |
A microsatellite linkage map of the European sea bass Dicentrarchus labrax L. Journal Article Genetics, 170 (4), pp. 1821–1826, 2005, ISSN: 00166731. |
Additional microsatellites for Sparus aurata and cross-species amplification within the Sparidae family Journal Article Molecular Ecology Notes, 5 , pp. 605 – 607, 2005. |
Marine Biology, 148 (2), pp. 319–326, 2005, ISSN: 00253162. |
Reviews in Fish Biology and Fisheries, 15 , pp. 53 – 73, 2005. |
Antimicrobial activity in bacteria isolated from Senegalese sole, Solea senegalensis, fed with natural prey Journal Article Aquaculture Research, 36 (16), pp. 1619–1627, 2005, ISSN: 1355557X. |
Comparative analysis and characterization of expressed sequence tags in gilthead sea bream (Sparus aurata) liver and embryos. Journal Article Aquaculture, 243 (1-4), pp. 69–81, 2005. |
Aquaculture, 246 (1-4), pp. 467–481, 2005, ISSN: 00448486, (Publisher: Elsevier). |
Composition of the organismic assemblage associated with Caretta caretta Journal Article Journal of the Marine Biological Association of the United Kingdom, 85 (2), pp. 257–261, 2005, ISSN: 00253154. |
Contribution of biomass burning to atmospheric polycyclic aromatic hydrocarbons at three european background sites Journal Article Environmental Science and Technology, 39 (9), pp. 2976–2982, 2005, ISSN: 0013936X. |
Design formulae for wave transmission behind floating breakwaters Inproceedings 2005, (Publication Title: XXXI IAHR Congress). |
Effect of dietary water on growth of dentex Dentex dentex Journal Article Fisheries Science, 71 (6), pp. 1243–1248, 2005, ISSN: 09199268. |
Estimating lagoonal biodiversity in Greece: Comparison of rapid assessment techniques Journal Article Helgoland Marine Research, 59 (3), pp. 177–186, 2005, ISSN: 1438387X. |
First results of greater amberjack (Seriola dumerili) larval rearing in mesocosm Journal Article Aquaculture, 250 (1-2), pp. 155–161, 2005, ISSN: 00448486. |
Floating breakwaters under regular and irregular wave forcing: reflection and transmission characteristics Journal Article Journal of Hydraulic Research, 43 (2), pp. 174–188, 2005, ISSN: 0022-1686, 1814-2079. |
Flow through a porous rubble mound weir Inproceedings pp. 2536–2542, 2005, (Publication Title: XXXI IAHR Congress). |
Physiological Genomics, 23 (2), pp. 182–191, 2005, ISSN: 10948341. |
Journal of Fish Biology, 66 (4), pp. 938–956, 2005, ISSN: 00221112, (Publisher: Blackwell Publishing Ltd). |
Impact of aquaculture on the genetic structure of mediterranean populations of Dicentrarchus labrax Journal Article Aquatic Living Resources, 18 (1), pp. 71–76, 2005, ISSN: 0990-7440, 1765-2952, (Original title (in French): Impact de l'élevage sur la structure génétique des populations méditerranéennes de Dicentrarchus labrax). |
Induction of ovulation and spawning in the Mediterranean Red Porgy, Pagrus Pagrus, by controlled delivery and acute injection of GnRHa Journal Article Israeli Journal of Aquaculture - Bamidgeh, 57 (4), pp. 223–230, 2005, ISSN: 0792156X. |
Journal of Fish Biology, 67 (3), pp. 652–668, 2005, ISSN: 00221112. |
Isolation and characterization of polymorphic microsatellite markers in the wall lizard Podarcis erhardii (Squamata: Lacertidae) Journal Article Molecular Ecology Notes, 5 (3), pp. 549–551, 2005. |
Isolation and characterization of six polymorphic microsatellite markers in the freshwater turtle Mauremys rivulata (Testudines: Geoemydidae) Journal Article Molecular Ecology Notes, 5 (4), pp. 727–729, 2005. |
Isolation, characterization and sequence determination of Noda virus from sea bass (Dicentrarchus labrax L.) reared in freshwater and marine facilities in Greece Journal Article Bulletin of the Hellenic Veterinary Medical Society, 56 (2), pp. 105–113, 2005. |
Mass budget and dynamics of polychlorinated biphenyls in the eastern Mediterranean Sea Journal Article Global Biogeochemical Cycles, 19 (3), pp. 1–16, 2005, ISSN: 08866236. |
Mediterranean lagoons revisited: Weakness and efficiency of the rapid biodiversity assessment techniques in a severely fluctuating environment Journal Article Biodiversity and Conservation, 14 (10), pp. 2347–2359, 2005, ISSN: 09603115. |
Modeling feeding processes: A test of a new model for sea bream (Sparus aurata) larvae Journal Article Canadian Journal of Fisheries and Aquatic Sciences, 62 , pp. 425 – 435, 2005. |
Bulletin of the European Association of Fish Pathologists, 25 (1), pp. 28–31, 2005, ISSN: 01080288. |
On the analysis of black carbon in sediments: Evaluation of field data for geochemical consistency Inproceedings Geophysical Research Abstracts, pp. EGU05–A–06089, 2005. |
Ontogeny of the shi drum Umbrina cirrosa (Linnaeus 1758), a candidate new species for aquaculture Journal Article Aquaculture Research, 36 (13), pp. 1265–1272, 2005, ISSN: 1355557X. |
Ontogeny of the thyroid hormones and cortisol in the gilthead sea bream, Sparus aurata Journal Article General and Comparative Endocrinology, 142 , pp. 186 – 192, 2005. |
Phylogenetic relationships and evolutionary history of snake-eyed skink Ablepharus kitaibelii (Sauria: Scincidae) Journal Article Molecular Phylogenetics and Evolution, 34 , pp. 245 – 256, 2005. |
Preliminary results on the effect of otter trawling on hyperbenthic communities in Heraklion Bay (Eastern Mediterranean, Cretan Sea) Journal Article American Fisheries Society Symposium, 41 , pp. 529–537, 2005. |
Bulletin of the European Association of Fish Pathologists, 25 (6), pp. 256–261, 2005, ISSN: 01080288. |
Continental Shelf Research, 25 , pp. 2196–2212, 2005. |
Successional stages of experimental artificial reefs deployed in Vistonikos gulf (North SAegean Sea, Greece): Preliminary results Journal Article Belgian Journal of Zoology, 135 , pp. 209–215, 2005. |
Supercritical free-surface turbulent flow over a semicircular obstacle Inproceedings pp. 1366–1376, 2005, (Publication Title: XXXI IAHR Congress). |
The effect of different carotenoid sources on skin coloration of cultured red porgy (Pagrus pagrus) Journal Article Aquaculture Research, 36 (15), pp. 1517–1525, 2005, ISSN: 1355557X. |
The effect of different types of otter trawl groundrope on benthic nutrient fluxes and sediment biogeochemistry Journal Article American Fisheries Society Symposium, 41 , pp. 539–544, 2005. |
The phylogeography of the gecko Cyrtopodion kotschyi (Reptilia: Gekkonidae) in the Aegean archipelago Journal Article Molecular Phylogenetics and Evolution, 35 (3), pp. 612–623, 2005, ISSN: 10557903. |
Molecular Phylogenetics and Evolution, 34 (1), pp. 55–66, 2005, ISSN: 10557903. |
The use of the polychaete worm Nereis virens eggs as vehicle for the delivery of oxytetracycline in Solea solea larvae Journal Article Aquaculture, 243 (1-4), pp. 1–7, 2005, ISSN: 00448486. |
2004 |
Sensory, microbiological, and chemical spoilage of cultured common sea bass ( Dicentrarchus labrax) stored in ice: a seasonal differentiation Journal Article European Food Research and Technology, 219 (6), pp. 584–587, 2004, ISSN: 1438-2377, 1438-2385. |
Development of a reference diet for use in indispensable amino acid requirement studies of gilthead seabream Sparus aurata L. Journal Article Aquaculture Nutrition, 10 (5), pp. 335–343, 2004, ISSN: 1353-5773, 1365-2095. |
Mortality control of VNN disease in 0+ grouper, Epinephelus marginatus after prolonged bath in dense Chlorella minutissima culture. Inproceedings EAS, (Ed.): European Aquaculture Society, Biotechnologies for Quality, Barcelona, Spain, 2004. |
The rearing of common dentex Dentex dentex (L.) During the hatchery and on-growing phases Journal Article Aquaculture, 240 (1-4), pp. 165–173, 2004, ISSN: 00448486. |
Floating Breakwater Response to Waves Action Using a Boussinesq Model Coupled with a 2DV Elliptic Solver Journal Article Journal of Waterway, Port, Coastal, and Ocean Engineering, 130 (5), pp. 243–255, 2004, ISSN: 0733-950X, 1943-5460. |
Stock discrimination and temporal and spatial genetic variation of sardine (Sardina pilchardus) in northeastern Atlantic, with a combined analysis of nuclear (microsatellites and allozymes) and mitochondrial DNA markers. Inproceedings Kasapidis, P (Ed.): 2004 ICES Annual Science Conference, 2004. |