2004 |
Papandroulakis, N; Suquet, M; Spedicato, M T; Machias, A; Fauvel, C; Divanach, P Feeding rates, growth performance and gametogenesis of wreckfish (Polyprion americanus) kept in captivity Journal Article Aquaculture International, 12 , pp. 395 – 407, 2004. @article{papandroulakis_feeding_2004, title = {Feeding rates, growth performance and gametogenesis of wreckfish (Polyprion americanus) kept in captivity}, author = {N Papandroulakis and M Suquet and M T Spedicato and A Machias and C Fauvel and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33749494732&partnerID=40&md5=452a6a205ec500f8fdcd8736ff3a50f1}, year = {2004}, date = {2004-01-01}, journal = {Aquaculture International}, volume = {12}, pages = {395 -- 407}, abstract = {Wreckfish (Polyprion americanus) is a panoceanic, antitropical distributed species. Despite the high market price, high growth rate, good quality flesh and the potential for aquaculture, research data are scarce. In this study, the growth performance and food intake was investigated in juvenile fish, while gametogenesis was studied in adult fish. Three groups were created from 19 individuals, which were collected during neustonic sampling, using fish aggregating devices, in association with floating objects and during tuna fishing with drift nets from Greece (Crete), Italy (Ionian Sea) and the Atlantic coast of France from September 1999 to March 2001. All individuals (initial weight between 0.5 g and 4.5kg) were reared in aquaculture facilities so as to monitor growth performance and food intake. Food (de-frozen fish - bogue, mackerel and squid - or moist pellets) was delivered ad libitum. Total length and wet weight were measured regularly. Daily food intake, the food conversion index and daily growth rate were calculated. Individuals, aging 0+, grew exceptionally fast, reaching 2.0kg (total length 50cm) in 12 months and 5.0kg (total length 65cm) in 24, while at later stages, the growth rate decreased and depended on the water temperature during rearing. Food consumption varied during the rearing period according to the developmental stage and the rearing conditions. The food conversion ratio ranged between 0.9 and 2.5 on dry weight basis during the period of the study. Gametogenesis was monitored in a separate stock of 15 individuals (11.7 ± 3.7 kg) by biopsis while blood samples were taken for steroid estimation. Increased oocyte diameter was observed in females from August to January, while plasma estradiol levels also increased from October to March. However, neither egg or sperm were collected from the stock. Young wreckfish presented a high growth rate in captivity, thus supporting the feasibility of the species for rearing. Further research, however, is required for the reproduction of the species. © 2004 Kluwer Academic Publishers.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Wreckfish (Polyprion americanus) is a panoceanic, antitropical distributed species. Despite the high market price, high growth rate, good quality flesh and the potential for aquaculture, research data are scarce. In this study, the growth performance and food intake was investigated in juvenile fish, while gametogenesis was studied in adult fish. Three groups were created from 19 individuals, which were collected during neustonic sampling, using fish aggregating devices, in association with floating objects and during tuna fishing with drift nets from Greece (Crete), Italy (Ionian Sea) and the Atlantic coast of France from September 1999 to March 2001. All individuals (initial weight between 0.5 g and 4.5kg) were reared in aquaculture facilities so as to monitor growth performance and food intake. Food (de-frozen fish - bogue, mackerel and squid - or moist pellets) was delivered ad libitum. Total length and wet weight were measured regularly. Daily food intake, the food conversion index and daily growth rate were calculated. Individuals, aging 0+, grew exceptionally fast, reaching 2.0kg (total length 50cm) in 12 months and 5.0kg (total length 65cm) in 24, while at later stages, the growth rate decreased and depended on the water temperature during rearing. Food consumption varied during the rearing period according to the developmental stage and the rearing conditions. The food conversion ratio ranged between 0.9 and 2.5 on dry weight basis during the period of the study. Gametogenesis was monitored in a separate stock of 15 individuals (11.7 ± 3.7 kg) by biopsis while blood samples were taken for steroid estimation. Increased oocyte diameter was observed in females from August to January, while plasma estradiol levels also increased from October to March. However, neither egg or sperm were collected from the stock. Young wreckfish presented a high growth rate in captivity, thus supporting the feasibility of the species for rearing. Further research, however, is required for the reproduction of the species. © 2004 Kluwer Academic Publishers. |
Mylonas, C C; Papandroulakis, N; Smboukis, A; Papadaki, M; Divanach, P Induction of spawning of cultured greater amberjack (Seriola dumerili) using GnRHa implants Journal Article Aquaculture, 237 (1-4), pp. 141–154, 2004, ISSN: 00448486. @article{mylonas_induction_2004, title = {Induction of spawning of cultured greater amberjack (Seriola dumerili) using GnRHa implants}, author = {C C Mylonas and N Papandroulakis and A Smboukis and M Papadaki and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-3142566876&doi=10.1016%2fj.aquaculture.2004.04.015&partnerID=40&md5=1d5f2c1607615bf23df2ad0e72aaa783}, doi = {10.1016/j.aquaculture.2004.04.015}, issn = {00448486}, year = {2004}, date = {2004-01-01}, journal = {Aquaculture}, volume = {237}, number = {1-4}, pages = {141--154}, abstract = {The objective of the current study was to examine the possibility of maturing Mediterranean greater amberjack (Seriola dumerili) in culture at two different facilities in Greece and inducing spawning using gonadotropin releasing-hormone agonist (GnRHa) implants. Males in both stocks (n=8) were in spermiating condition in mid June, though sperm motility was low ranging between 5% and 30%, while motility duration ranged between 2.1 and 2.5 min. Females of the first stock (n=4) at the same time had ovaries with either primary oocytes or early vitellogenic oocytes at advanced stages of atresia, indicating a failure in oogenesis. On the contrary, the only female of the second stock contained oocytes at all stages of oogenesis, including early final oocyte maturation (FOM; oocyte diameter 650 μm). Implantation with a GnRHa implant induced the first spawn about 36 h later. In the absence of another spawn, a gonadal biopsy was taken 15 days later, indicating the existence of more oocytes at post vitellogenesis and some with almost complete lipid droplet coalescence. A second GnRHa implantation at this time resulted in consecutive egg releases after 36 h, 4 and 5 days, with fertilized eggs only at 36 h and 5 days. The study underlines the existence of important reproductive dysfunctions of greater amberjack in captivity, and demonstrates that GnRHa implants can be used for the induction of multiple spawns of viable eggs. © 2004 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The objective of the current study was to examine the possibility of maturing Mediterranean greater amberjack (Seriola dumerili) in culture at two different facilities in Greece and inducing spawning using gonadotropin releasing-hormone agonist (GnRHa) implants. Males in both stocks (n=8) were in spermiating condition in mid June, though sperm motility was low ranging between 5% and 30%, while motility duration ranged between 2.1 and 2.5 min. Females of the first stock (n=4) at the same time had ovaries with either primary oocytes or early vitellogenic oocytes at advanced stages of atresia, indicating a failure in oogenesis. On the contrary, the only female of the second stock contained oocytes at all stages of oogenesis, including early final oocyte maturation (FOM; oocyte diameter 650 μm). Implantation with a GnRHa implant induced the first spawn about 36 h later. In the absence of another spawn, a gonadal biopsy was taken 15 days later, indicating the existence of more oocytes at post vitellogenesis and some with almost complete lipid droplet coalescence. A second GnRHa implantation at this time resulted in consecutive egg releases after 36 h, 4 and 5 days, with fertilized eggs only at 36 h and 5 days. The study underlines the existence of important reproductive dysfunctions of greater amberjack in captivity, and demonstrates that GnRHa implants can be used for the induction of multiple spawns of viable eggs. © 2004 Elsevier B.V. All rights reserved. |
Zampicinini, G; Blinov, A; Cervella, P; Guryev, V; Sella, G Genome, 47 , pp. 1154–1163, 2004. @article{zampicinini_insertional_2004, title = {Insertional polymorphism of a non-LTR mobile element (NLRCth1) in European populations of Chironomus riparius (Diptera, Chironomidae) as detected by transposon insertion display}, author = {G Zampicinini and A Blinov and P Cervella and V Guryev and G Sella}, url = {http://www.nrcresearchpress.com/doi/abs/10.1139/g04-066}, doi = {10.1139/g04-066}, year = {2004}, date = {2004-01-01}, journal = {Genome}, volume = {47}, pages = {1154--1163}, abstract = {The midge Chironomus riparius is distributed all over the Palearctic region and is well characterized both at the morphological and cytogenetic levels. Here we describe a population study based on the insertional polymorphism of the retroposon NLRCth1, by means of a S-SAP (sequence-specific amplification polymorphism) derived technique (transposon insertion display; TID). While a previous study of allozyme polymorphism in Russian samples showed little variability, all the amplicons we identified are polymorphic. Genetic distances between 6 natural populations were calculated according to Nei and did not show a positive correlation with geographic distances. The genetic diversity detected among individuals of a given population was one order of magnitude higher than that among populations. However, the value of ΦST was significant (p < 0.001) and indicates that natural populations are more genetically differentiated than random samples of individuals. © 2004 NRC Canada.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The midge Chironomus riparius is distributed all over the Palearctic region and is well characterized both at the morphological and cytogenetic levels. Here we describe a population study based on the insertional polymorphism of the retroposon NLRCth1, by means of a S-SAP (sequence-specific amplification polymorphism) derived technique (transposon insertion display; TID). While a previous study of allozyme polymorphism in Russian samples showed little variability, all the amplicons we identified are polymorphic. Genetic distances between 6 natural populations were calculated according to Nei and did not show a positive correlation with geographic distances. The genetic diversity detected among individuals of a given population was one order of magnitude higher than that among populations. However, the value of ΦST was significant (p < 0.001) and indicates that natural populations are more genetically differentiated than random samples of individuals. © 2004 NRC Canada. |
Atienza, M T; Chatzifotis, S; Divanach, P Macronutrient selection by sharp snout seabream (Diplodus puntazzo) Journal Article Aquaculture, 232 (1-4), pp. 481–491, 2004, ISSN: 00448486. @article{atienza_macronutrient_2004, title = {Macronutrient selection by sharp snout seabream (Diplodus puntazzo)}, author = {M T Atienza and S Chatzifotis and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-1542321232&doi=10.1016%2fS0044-8486%2803%2900468-X&partnerID=40&md5=7f2fd0488cac53aa1a23cefd86f2ffa5}, doi = {10.1016/S0044-8486(03)00468-X}, issn = {00448486}, year = {2004}, date = {2004-01-01}, journal = {Aquaculture}, volume = {232}, number = {1-4}, pages = {481--491}, abstract = {This study is concerned with the investigation of feed preference in juvenile sharp snout seabream (Diplodus puntazzo). Three isoenergetic diets, high protein-low fat (HPLF), medium protein and fat (MPMF) and high fat-low protein (LPHF) were tested using six feeding regimes. Three groups of fish were used as controls to test the diets independently, while to the other three, a different diet combination was provided (HPLF-MPMF, MPMF-LPHF and HPLF-LPHF). Diets were provided by means of one or two self-feeders and fish had continuous access to one or two diets. Fish had an initial weight of 11.1±3.8 g (mean±S.D.) and after 45 days of feeding, no significant differences (Ptextless0.05) was observed in specific growth rate (SGR), feed conversion ratio (FCR) and protein efficiency ratio (PER) among different groups. Fish on HPLF-MPMF and HPLF-LPHF feeding regimes showed the highest growth rate of 1.96% per day and the lowest feed conversion ratio of 1.09, respectively. The result obtained showed that although there was different availability of diets among groups, macronutrient consumption appears to converge suggesting a target intake (47% protein and 10% fat content). The protein content of the diet influenced feed consumption, growth and feed conversion ratio. © 2004 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study is concerned with the investigation of feed preference in juvenile sharp snout seabream (Diplodus puntazzo). Three isoenergetic diets, high protein-low fat (HPLF), medium protein and fat (MPMF) and high fat-low protein (LPHF) were tested using six feeding regimes. Three groups of fish were used as controls to test the diets independently, while to the other three, a different diet combination was provided (HPLF-MPMF, MPMF-LPHF and HPLF-LPHF). Diets were provided by means of one or two self-feeders and fish had continuous access to one or two diets. Fish had an initial weight of 11.1±3.8 g (mean±S.D.) and after 45 days of feeding, no significant differences (Ptextless0.05) was observed in specific growth rate (SGR), feed conversion ratio (FCR) and protein efficiency ratio (PER) among different groups. Fish on HPLF-MPMF and HPLF-LPHF feeding regimes showed the highest growth rate of 1.96% per day and the lowest feed conversion ratio of 1.09, respectively. The result obtained showed that although there was different availability of diets among groups, macronutrient consumption appears to converge suggesting a target intake (47% protein and 10% fat content). The protein content of the diet influenced feed consumption, growth and feed conversion ratio. © 2004 Elsevier B.V. All rights reserved. |
Papandroulakis, N; Kentouri, M; Maingot, E; Divanach, P Mesocosm: A reliable technology for larval rearing of Diplodus puntazzo and Diplodus sargus sargus Journal Article Aquaculture International, 12 , pp. 345 – 355, 2004. @article{papandroulakis_mesocosm_2004, title = {Mesocosm: A reliable technology for larval rearing of Diplodus puntazzo and Diplodus sargus sargus}, author = {N Papandroulakis and M Kentouri and E Maingot and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33644987838&partnerID=40&md5=76b681983b3ee6f2281fdabaab3a4b3d}, year = {2004}, date = {2004-01-01}, journal = {Aquaculture International}, volume = {12}, pages = {345 -- 355}, abstract = {Species diversification is today considered as a major issue for the sustainable development of the Mediterranean aquaculture. For successful propagation of any species however, larval rearing is considered a bottleneck and therefore the development of appropriate tools is essential. Mesocosm is a semi-intensive technology that facilitates larval rearing of several species integrating principles of both intensive and extensive aquaculture, which solves biological problems and many of their technical, human and economical consequences. The extensive (and now even the semi-extensive) strategy is used in the most critical segments of the rearing process during the early developmental stages, when larvae are still extremely weak, sensitive to intensive environment, easily stressed and difficult to feed. The intensive strategy is used as soon as larvae are considered mature enough to be reared easily using classical methods. The technology was used for the rearing of two species, with potential for aquaculture, the sharpsnout seabream (Diplodus puntazzo) and the white seabream (Diplodus sargus sargus). Three groups of each species were monitored for a period of 50-70 days post hatching. Survival for both species was about 54% at the end of the trials. Sharpsnout seabream larvae reached 19.6 ± 0.9 mm total length and 107.2±31.9mg body weight 50 days post hatching. White seabream larvae 60 days post hatching reached 32.7 ± 2.7 mm total length and 450±70mg body weight. In order to verify the economical viability of the technology, the individual production cost for each species was estimated and reached €0.027 for white seabream and €0.043 for sharpsnout seabream. Results indicate the reliability of the technology for the larval rearing of the two species. © 2004 Kluwer Academic Publishers.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Species diversification is today considered as a major issue for the sustainable development of the Mediterranean aquaculture. For successful propagation of any species however, larval rearing is considered a bottleneck and therefore the development of appropriate tools is essential. Mesocosm is a semi-intensive technology that facilitates larval rearing of several species integrating principles of both intensive and extensive aquaculture, which solves biological problems and many of their technical, human and economical consequences. The extensive (and now even the semi-extensive) strategy is used in the most critical segments of the rearing process during the early developmental stages, when larvae are still extremely weak, sensitive to intensive environment, easily stressed and difficult to feed. The intensive strategy is used as soon as larvae are considered mature enough to be reared easily using classical methods. The technology was used for the rearing of two species, with potential for aquaculture, the sharpsnout seabream (Diplodus puntazzo) and the white seabream (Diplodus sargus sargus). Three groups of each species were monitored for a period of 50-70 days post hatching. Survival for both species was about 54% at the end of the trials. Sharpsnout seabream larvae reached 19.6 ± 0.9 mm total length and 107.2±31.9mg body weight 50 days post hatching. White seabream larvae 60 days post hatching reached 32.7 ± 2.7 mm total length and 450±70mg body weight. In order to verify the economical viability of the technology, the individual production cost for each species was estimated and reached €0.027 for white seabream and €0.043 for sharpsnout seabream. Results indicate the reliability of the technology for the larval rearing of the two species. © 2004 Kluwer Academic Publishers. |
Magoulas, A Mitochondrial DNA Incollection Cadrin, S; Friedland, K; Waldman, J (Ed.): Stock Ιdentification Μethods, pp. 311–330, ELSEVIER Academic Press, 2004, (Backup Publisher: ELSEVIER Academic Press Section: 16). @incollection{magoulas_mitochondrial_2004, title = {Mitochondrial DNA}, author = {A Magoulas}, editor = {S Cadrin and K Friedland and J Waldman}, year = {2004}, date = {2004-01-01}, booktitle = {Stock Ιdentification Μethods}, pages = {311--330}, publisher = {ELSEVIER Academic Press}, note = {Backup Publisher: ELSEVIER Academic Press Section: 16}, keywords = {}, pubstate = {published}, tppubtype = {incollection} } |
Katharios, P; Iliopoulou-Georgudaki, J; Antimisiaris, S; Verri, T; Toma, P; Acierno, R; Maffia, M Pharmacokinetics of cephalexin in sea bream, Sparus aurata (L.), after a single intraperitoneal injection Journal Article Journal of Applied Ichthyology, 20 (5), pp. 422–426, 2004, ISSN: 01758659. @article{katharios_pharmacokinetics_2004, title = {Pharmacokinetics of cephalexin in sea bream, Sparus aurata (L.), after a single intraperitoneal injection}, author = {P Katharios and J Iliopoulou-Georgudaki and S Antimisiaris and T Verri and P Toma and R Acierno and M Maffia}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-5444259750&doi=10.1111%2fj.1439-0426.2004.00578.x&partnerID=40&md5=7ca32ad32162890875c6aca6aff4f216}, doi = {10.1111/j.1439-0426.2004.00578.x}, issn = {01758659}, year = {2004}, date = {2004-01-01}, journal = {Journal of Applied Ichthyology}, volume = {20}, number = {5}, pages = {422--426}, abstract = {The pharmacokinetic properties of cephalexin were studied in sea bream (mean weight 77 g), Sparus aurata (L.) after a single intraperitoneal injection (200 mg kg-1). Pharmacokinetic analysis of the serum concentrations vs time points obtained was performed using non-compartmental analysis and a compartmental pharmacokinetic model approach. In the latter case, a two-compartment open model with a lag time gave the best fitting. The maximum peak serum concentration was 5.018 mg ml-1 kg-1 1 h post-treatment. The area under the curve (AUC) cephalexin was 17.394 mg·h kg-1 and the elimination half-life of 1.83 h. © 2004 Blackwell Verlag, Berlin.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The pharmacokinetic properties of cephalexin were studied in sea bream (mean weight 77 g), Sparus aurata (L.) after a single intraperitoneal injection (200 mg kg-1). Pharmacokinetic analysis of the serum concentrations vs time points obtained was performed using non-compartmental analysis and a compartmental pharmacokinetic model approach. In the latter case, a two-compartment open model with a lag time gave the best fitting. The maximum peak serum concentration was 5.018 mg ml-1 kg-1 1 h post-treatment. The area under the curve (AUC) cephalexin was 17.394 mg·h kg-1 and the elimination half-life of 1.83 h. © 2004 Blackwell Verlag, Berlin. |
Rigos, G; Tyrpenou, ; Nengas, I; Alexis, A E M; Poulou, Athanasso F; Troisi, G M Poor bioavailability of oxytetracycline in sharpsnout sea bream Diplodus puntazzo Journal Article Aquaculture, 235 , pp. 489 – 497, 2004. @article{rigos_poor_2004, title = {Poor bioavailability of oxytetracycline in sharpsnout sea bream Diplodus puntazzo}, author = {G Rigos and Tyrpenou and I Nengas and A E M Alexis and F Athanasso Poulou and G M Troisi}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-1542280919&partnerID=40&md5=7c1f6e8e157f8928bcb1086f90bea99a}, year = {2004}, date = {2004-01-01}, journal = {Aquaculture}, volume = {235}, pages = {489 -- 497}, abstract = {The pharmacokinetics of oxytetracycline (OTC) were investigated following single intravascular injection (40 mg/kg) in sharpsnout sea bream (90 g) at 19°C. The distribution half-life (t1/2α) and the elimination half-life (t1/2β) of OTC were calculated to be 1.4 and 35 h, respectively. The apparent volume of distribution of the drug at steady-state (Vd(ss)) was found to be 4 l/kg. The total clearance rate (CLT) of the drug was low (0.08 l/kg h). Repeated attempts to investigate the bioavailability of OTC following oral administration (75 mg/kg; forced-fed or via the feed), revealed undetectable levels of the drug in plasma and muscle samples. Poor tissue absorption is likely due to significant hepatic metabolism of OTC in sharpsnout sea bream, but this requires further investigation. Thus, oral administration of OTC in farmed sharpsnout sea bream should be discouraged in favour of other routes of administration or other antibiotic drugs. © 2003 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The pharmacokinetics of oxytetracycline (OTC) were investigated following single intravascular injection (40 mg/kg) in sharpsnout sea bream (90 g) at 19°C. The distribution half-life (t1/2α) and the elimination half-life (t1/2β) of OTC were calculated to be 1.4 and 35 h, respectively. The apparent volume of distribution of the drug at steady-state (Vd(ss)) was found to be 4 l/kg. The total clearance rate (CLT) of the drug was low (0.08 l/kg h). Repeated attempts to investigate the bioavailability of OTC following oral administration (75 mg/kg; forced-fed or via the feed), revealed undetectable levels of the drug in plasma and muscle samples. Poor tissue absorption is likely due to significant hepatic metabolism of OTC in sharpsnout sea bream, but this requires further investigation. Thus, oral administration of OTC in farmed sharpsnout sea bream should be discouraged in favour of other routes of administration or other antibiotic drugs. © 2003 Elsevier B.V. All rights reserved. |
Rigos, G; Nengas, I; Alexis, M; Troisi, G M Potential drug (oxytetracycline and oxolinic acid) pollution from Mediterranean sparid fish farms Journal Article Aquatic Toxicology, 69 , pp. 281 – 288, 2004. @article{rigos_potential_2004, title = {Potential drug (oxytetracycline and oxolinic acid) pollution from Mediterranean sparid fish farms}, author = {G Rigos and I Nengas and M Alexis and G M Troisi}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-3242806075&partnerID=40&md5=bca8f73fe1a7ba2abb13d5ad230e6027}, year = {2004}, date = {2004-01-01}, journal = {Aquatic Toxicology}, volume = {69}, pages = {281 -- 288}, abstract = {The potential for input of two common antibacterial agents in Mediterranean fish farms, oxytetracycline (OTC) and oxolinic acid (OA), was estimated from measurements of these drugs in the faecal excretions of two important farmed sparids, gilthead sea bream, Sparus aurata and sharpsnout sea bream Diplodus puntazzo. Oxolinic acid was found to be well absorbed by gilthead sea bream (92%) and sharpsnout sea bream (88%) while the absorption of OTC was found to be considerably lower in both species (27 and 40%, respectively). These data were integrated with production records for sparids, drug dosage regimes and treatment frequency information to calculate potential annual drug release to the aquatic environment from Greek fish farms. These calculations suggest potentially significant quantities of unmetabolised OTC can be passed unabsorbed through the body of treated sparids and excreted via the faeces into the local marine environment. The situation with OA was much less pronounced. It was estimated that potentially more than 1900 kg of OTC and more than 50 kg of OA may be released via faecal excretion into the environment by sparid farms per year. Further drug may also be released via uneaten medicated feed, leached drugs and other routes of fish elimination (renal excretion, branchial secretions). Drug pollution of the marine environment in the vicinity of fish farms can have adverse ecological effects, including development of resistant bacterial populations and exposure with potential drug accumulation in aquatic fauna and flora. © 2004 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The potential for input of two common antibacterial agents in Mediterranean fish farms, oxytetracycline (OTC) and oxolinic acid (OA), was estimated from measurements of these drugs in the faecal excretions of two important farmed sparids, gilthead sea bream, Sparus aurata and sharpsnout sea bream Diplodus puntazzo. Oxolinic acid was found to be well absorbed by gilthead sea bream (92%) and sharpsnout sea bream (88%) while the absorption of OTC was found to be considerably lower in both species (27 and 40%, respectively). These data were integrated with production records for sparids, drug dosage regimes and treatment frequency information to calculate potential annual drug release to the aquatic environment from Greek fish farms. These calculations suggest potentially significant quantities of unmetabolised OTC can be passed unabsorbed through the body of treated sparids and excreted via the faeces into the local marine environment. The situation with OA was much less pronounced. It was estimated that potentially more than 1900 kg of OTC and more than 50 kg of OA may be released via faecal excretion into the environment by sparid farms per year. Further drug may also be released via uneaten medicated feed, leached drugs and other routes of fish elimination (renal excretion, branchial secretions). Drug pollution of the marine environment in the vicinity of fish farms can have adverse ecological effects, including development of resistant bacterial populations and exposure with potential drug accumulation in aquatic fauna and flora. © 2004 Elsevier B.V. All rights reserved. |
Mandalakis, M; Gustafsson, Ö; Reddy, C M; Xu, L Radiocarbon apportionment of fossil versus biofuel combustion sources of polycyclic aromatic hydrocarbons in the Stockholm metropolitan area Journal Article Environmental Science and Technology, 38 (20), pp. 5344–5349, 2004, ISSN: 0013936X. @article{mandalakis_radiocarbon_2004, title = {Radiocarbon apportionment of fossil versus biofuel combustion sources of polycyclic aromatic hydrocarbons in the Stockholm metropolitan area}, author = {M Mandalakis and Ö Gustafsson and C M Reddy and L Xu}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-6044256299&doi=10.1021%2fes049088x&partnerID=40&md5=dada99d9431117db76b594464cebbbb1}, doi = {10.1021/es049088x}, issn = {0013936X}, year = {2004}, date = {2004-01-01}, journal = {Environmental Science and Technology}, volume = {38}, number = {20}, pages = {5344--5349}, abstract = {Source-diagnostic markers and the isotopic composition of polycyclic aromatic hydrocarbons (PAHs) were examined in surface sediments from the greater Stockholm waterways to deduce the contribution from biomass sources to the environmental PAH load. The summed concentration of 20 PAHs ranged from 0.8 to 45.1 μg/g (dry weight) and exhibited a steep decline with increasing distance from the city center evidencing that sources within the metropolitan area of Stockholm dominate its PAH burden. Several diagnostic PAH ratios indicated an overwhelming predominance of pyrogenic sources over the petrogenic ones, while retene and 1,7-dimethylphenanthrene were unable to correctly evaluate the contribution from biomass combustion. The stable carbon isotope composition (δ13C) of individual PAHs ranged from -24.8 to -27.0% but also was proved inefficient to discriminate between different types of fuels due to the overlapping signals in various sources. The Δ14C values of PAHs ranged between -550.4 and -934.1%, indicating a clear predominance of fossil fuel sources. By using an isotopic mass balance approach, we estimated that on average 17 ± 9% of PAHs derived from biomass combustion. This radiocarbon apportionment, in conjunction with detailed energy statistics for the Stockholm region, revealed that the ambient PAH burden is roughly similar, per unit energy produced, from fossil fuels and biofuels. Societies' shifting energy policies toward a larger reliance on biofuels may thus not lead to further deterioration of air quality and respiratory ailments for the urban population.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Source-diagnostic markers and the isotopic composition of polycyclic aromatic hydrocarbons (PAHs) were examined in surface sediments from the greater Stockholm waterways to deduce the contribution from biomass sources to the environmental PAH load. The summed concentration of 20 PAHs ranged from 0.8 to 45.1 μg/g (dry weight) and exhibited a steep decline with increasing distance from the city center evidencing that sources within the metropolitan area of Stockholm dominate its PAH burden. Several diagnostic PAH ratios indicated an overwhelming predominance of pyrogenic sources over the petrogenic ones, while retene and 1,7-dimethylphenanthrene were unable to correctly evaluate the contribution from biomass combustion. The stable carbon isotope composition (δ13C) of individual PAHs ranged from -24.8 to -27.0% but also was proved inefficient to discriminate between different types of fuels due to the overlapping signals in various sources. The Δ14C values of PAHs ranged between -550.4 and -934.1%, indicating a clear predominance of fossil fuel sources. By using an isotopic mass balance approach, we estimated that on average 17 ± 9% of PAHs derived from biomass combustion. This radiocarbon apportionment, in conjunction with detailed energy statistics for the Stockholm region, revealed that the ambient PAH burden is roughly similar, per unit energy produced, from fossil fuels and biofuels. Societies' shifting energy policies toward a larger reliance on biofuels may thus not lead to further deterioration of air quality and respiratory ailments for the urban population. |
Mylonas, C C; Kyriakou, Y; Sigelaki, I; Georgiou, G; Stephanou, D; Divanach, P Reproductive biology of the shi drum (Umrina cirrosa) in captivity and induction of spawning using GNRHA Journal Article Israeli Journal of Aquaculture - Bamidgeh, 56 (2), pp. 77–94, 2004, ISSN: 0792156X. @article{mylonas_reproductive_2004, title = {Reproductive biology of the shi drum (Umrina cirrosa) in captivity and induction of spawning using GNRHA}, author = {C C Mylonas and Y Kyriakou and I Sigelaki and G Georgiou and D Stephanou and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-3142760090&partnerID=40&md5=9f2ed2f2e75086d9b738a1ab4dedd38d}, issn = {0792156X}, year = {2004}, date = {2004-01-01}, journal = {Israeli Journal of Aquaculture - Bamidgeh}, volume = {56}, number = {2}, pages = {77--94}, abstract = {The reproductive biology of the shi drum (Umbrina cirrosa) in culture was histologically examined and sperm quality was monitored during an entire reproductive period. Already in April, the ovary contained oocytes in all stages of maturation, from primary oocytes to full vitellogenesis, as expected from a group-synchronous multiple-batch spawning fish. Vitellogenesis of the first batch of oocytes occurred very rapidly and their mean diameter (500 μm) did not increase significantly (p>0.05) as the reproductive period proceeded. The spermiation index peaked in May-June, but fish never produced copious amounts of milt upon abdominal pressure. The spermatozoa motility percentage remained unchanged throughout the spawning season (80%) and a significant percentage (40%) maintained viability after overnight storage at 4°C. Sperm density and motility duration increased during the reproductive period and varied 13-26 × 109 spermatozoa/ml and 26-40 s, respectively. Spontaneous spawning was not observed during the two-year study. Injection of post-vitellogenic females with an agonist of gonadotropin-releasing hormone (GnRHa) was successful in inducing a single spawning after two days, with fertilization, hatching and 4-day larval survival rates of 65%, 42-76% and 46-80%, respectively. The results underline the failure of female shi drum in culture to undergo final oocyte maturation and, although GnRHa injection was effective in inducing spawning of viable eggs, multiple treatments did not induce multiple spawns, as was expected from fish with multiple-batch group-synchronous ovarian biology.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The reproductive biology of the shi drum (Umbrina cirrosa) in culture was histologically examined and sperm quality was monitored during an entire reproductive period. Already in April, the ovary contained oocytes in all stages of maturation, from primary oocytes to full vitellogenesis, as expected from a group-synchronous multiple-batch spawning fish. Vitellogenesis of the first batch of oocytes occurred very rapidly and their mean diameter (500 μm) did not increase significantly (p>0.05) as the reproductive period proceeded. The spermiation index peaked in May-June, but fish never produced copious amounts of milt upon abdominal pressure. The spermatozoa motility percentage remained unchanged throughout the spawning season (80%) and a significant percentage (40%) maintained viability after overnight storage at 4°C. Sperm density and motility duration increased during the reproductive period and varied 13-26 × 109 spermatozoa/ml and 26-40 s, respectively. Spontaneous spawning was not observed during the two-year study. Injection of post-vitellogenic females with an agonist of gonadotropin-releasing hormone (GnRHa) was successful in inducing a single spawning after two days, with fertilization, hatching and 4-day larval survival rates of 65%, 42-76% and 46-80%, respectively. The results underline the failure of female shi drum in culture to undergo final oocyte maturation and, although GnRHa injection was effective in inducing spawning of viable eggs, multiple treatments did not induce multiple spawns, as was expected from fish with multiple-batch group-synchronous ovarian biology. |
Rigos, G; Tyrpenou, A E; Nengas, I; Alexis, M; Troisi, G M The kinetic profile of oxolinic acid in sharpsnout sea bream, Diplodus puntazzo (Cetti 1777) Journal Article Aquaculture Research, 35 , pp. 1299 – 1304, 2004. @article{rigos_kinetic_2004, title = {The kinetic profile of oxolinic acid in sharpsnout sea bream, Diplodus puntazzo (Cetti 1777)}, author = {G Rigos and A E Tyrpenou and I Nengas and M Alexis and G M Troisi}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-9344262875&partnerID=40&md5=747d42b180b9ebf50171888f16b799b3}, year = {2004}, date = {2004-01-01}, journal = {Aquaculture Research}, volume = {35}, pages = {1299 -- 1304}, abstract = {The pharmacokinetics of oxolinic acid (OA) were investigated after a single intra-vascular injection (20 mg kg-1 fish) in sharpsnout sea bream (90 g), a promising new euryhaline species for Mediterranean fish farming. The distribution half-life (t1/2α) and the elimination half-life (t1/2β) of OA were calculated to be 0.4 and 10 h respectively. The apparent volume of distribution at steady-state (Vd(ss)) and total clearance rate (CLT) of the drug were found to be 2.1 L kg and 0.2 L kg-1 h-1 respectively. The bioavailability (F%) of OA following oral administration (40 mg kg-1 fish) fish) was estimated to be 15%. The results indicate a rapid distribution and elimination of the drug, moderate tissue penetration, but low absorption in sharpsnout sea bream. The kinetic profile of OA found in this species is comparable with that observed in another well-known sparid, gilthead sea bream.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The pharmacokinetics of oxolinic acid (OA) were investigated after a single intra-vascular injection (20 mg kg-1 fish) in sharpsnout sea bream (90 g), a promising new euryhaline species for Mediterranean fish farming. The distribution half-life (t1/2α) and the elimination half-life (t1/2β) of OA were calculated to be 0.4 and 10 h respectively. The apparent volume of distribution at steady-state (Vd(ss)) and total clearance rate (CLT) of the drug were found to be 2.1 L kg and 0.2 L kg-1 h-1 respectively. The bioavailability (F%) of OA following oral administration (40 mg kg-1 fish) fish) was estimated to be 15%. The results indicate a rapid distribution and elimination of the drug, moderate tissue penetration, but low absorption in sharpsnout sea bream. The kinetic profile of OA found in this species is comparable with that observed in another well-known sparid, gilthead sea bream. |
Mandalakis, M; Stephanou, E G Wet deposition of polychlorinated biphenyls in the eastern Mediterranean Journal Article Environmental Science and Technology, 38 (11), pp. 3011–3018, 2004, ISSN: 0013936X. @article{mandalakis_wet_2004, title = {Wet deposition of polychlorinated biphenyls in the eastern Mediterranean}, author = {M Mandalakis and E G Stephanou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-2542447209&doi=10.1021%2fes030078q&partnerID=40&md5=62f74bf720be4dfe07eb56dbbd3b4ed2}, doi = {10.1021/es030078q}, issn = {0013936X}, year = {2004}, date = {2004-01-01}, journal = {Environmental Science and Technology}, volume = {38}, number = {11}, pages = {3011--3018}, abstract = {The concentrations of polychlorinated biphenyls (PCBs) were measured in rain samples collected from a semi-urban and a marine background site of the eastern Mediterranean. The concentration of ΣPCB (sum of 54 PCB congeners) in the city of Heraklion (2.9 ± 1.9 ng L-1) was not significantly higher than the corresponding concentration measured at the background sampling station of Finokalia (1.9 ± 0.9 ng L-1). In both sites, the sum of tri- and tetrachlorinated congeners accounted for more than 55% of ΣPCB in rainwater. For all samples, the percentage of particle-bound PCBs ranged between 6.6% and 63.8%, providing an average value of 31 ± 18%. The washout ratios of particulate PCBs (Wp) were constant for individual congeners regardless the degree of chlorination. Average W P values ranged between 1.9 × 105 and 5.2 × 105 while a value of 2.7(±1.3) × 105 was deduced for ΣPCB. The corresponding washout ratios for gaseous PCBs were substantially lower and ranged between 7 × 103 (PCB 99) and 1. 3 × 105 (PCB 180). Washout ratios of gaseous PCBs were also calculated based on Henry's law, and they were found to be 30-920 times lower than those obtained from field measurements. On the basis of our data, the wet deposition flux of ΣPCB in the eastern Mediterranean should approach 820 ng m-2 yr-1. This flux is similar with the values recently reported for several background sites of the United States and Europe, but it is 1 order of magnitude lower than the flux of PCBs measured in the western Mediterranean 16 yr ago.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The concentrations of polychlorinated biphenyls (PCBs) were measured in rain samples collected from a semi-urban and a marine background site of the eastern Mediterranean. The concentration of ΣPCB (sum of 54 PCB congeners) in the city of Heraklion (2.9 ± 1.9 ng L-1) was not significantly higher than the corresponding concentration measured at the background sampling station of Finokalia (1.9 ± 0.9 ng L-1). In both sites, the sum of tri- and tetrachlorinated congeners accounted for more than 55% of ΣPCB in rainwater. For all samples, the percentage of particle-bound PCBs ranged between 6.6% and 63.8%, providing an average value of 31 ± 18%. The washout ratios of particulate PCBs (Wp) were constant for individual congeners regardless the degree of chlorination. Average W P values ranged between 1.9 × 105 and 5.2 × 105 while a value of 2.7(±1.3) × 105 was deduced for ΣPCB. The corresponding washout ratios for gaseous PCBs were substantially lower and ranged between 7 × 103 (PCB 99) and 1. 3 × 105 (PCB 180). Washout ratios of gaseous PCBs were also calculated based on Henry's law, and they were found to be 30-920 times lower than those obtained from field measurements. On the basis of our data, the wet deposition flux of ΣPCB in the eastern Mediterranean should approach 820 ng m-2 yr-1. This flux is similar with the values recently reported for several background sites of the United States and Europe, but it is 1 order of magnitude lower than the flux of PCBs measured in the western Mediterranean 16 yr ago. |
2003 |
Bargelloni, L; Alarcon, J A; Alvarez, M C; Penzo, E; Magoulas, A; Reis, C; Patarnello, T Discord in the family Sparidae (Teleostei): divergent phylogeographical patterns across the Atlantic-Mediterranean divide Journal Article Journal of Evolutionary Biology, 16 (6), pp. 1149–1158, 2003, ISSN: 1010-061X, 1420-9101. @article{bargelloni_discord_2003, title = {Discord in the family Sparidae (Teleostei): divergent phylogeographical patterns across the Atlantic-Mediterranean divide}, author = {L Bargelloni and J A Alarcon and M C Alvarez and E Penzo and A Magoulas and C Reis and T Patarnello}, url = {http://doi.wiley.com/10.1046/j.1420-9101.2003.00620.x}, doi = {10.1046/j.1420-9101.2003.00620.x}, issn = {1010-061X, 1420-9101}, year = {2003}, date = {2003-11-01}, urldate = {2020-08-09}, journal = {Journal of Evolutionary Biology}, volume = {16}, number = {6}, pages = {1149--1158}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Martyniuk, C J; Perry, G M L; Mogahadam, H K; Ferguson, M M; Danzmann, R G The genetic architecture of correlations among growth-related traits and male age at maturation in rainbow trout Journal Article Journal of Fish Biology, 63 (3), pp. 746–764, 2003, ISSN: 0022-1112, 1095-8649. @article{martyniuk_genetic_2003, title = {The genetic architecture of correlations among growth-related traits and male age at maturation in rainbow trout}, author = {C J Martyniuk and G M L Perry and H K Mogahadam and M M Ferguson and R G Danzmann}, url = {http://doi.wiley.com/10.1046/j.1095-8649.2003.00188.x}, doi = {10.1046/j.1095-8649.2003.00188.x}, issn = {0022-1112, 1095-8649}, year = {2003}, date = {2003-09-01}, urldate = {2020-08-11}, journal = {Journal of Fish Biology}, volume = {63}, number = {3}, pages = {746--764}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Manios, Andreas; Tosca, Androniki; Volakakis, Evaggelos; Leivadara, Moshoula; Tsiftsis, Dimitris Computer assisted evaluation of wound healing in chronic ulcers Journal Article Computers in Biology and Medicine, 33 (4), pp. 311–317, 2003, ISSN: 00104825. @article{manios_computer_2003, title = {Computer assisted evaluation of wound healing in chronic ulcers}, author = {Andreas Manios and Androniki Tosca and Evaggelos Volakakis and Moshoula Leivadara and Dimitris Tsiftsis}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0010482502000823}, doi = {10.1016/S0010-4825(02)00082-3}, issn = {00104825}, year = {2003}, date = {2003-07-01}, urldate = {2020-08-17}, journal = {Computers in Biology and Medicine}, volume = {33}, number = {4}, pages = {311--317}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Venou, B; Alexis, M N; Fountoulaki, E; Nengas, I; Apostolopoulou, M; Castritsi-Cathariou, I Aquaculture, 225 (1-4), pp. 207–223, 2003, ISSN: 00448486. @article{venou_effect_2003, title = {Effect of extrusion of wheat and corn on gilthead sea bream (Sparus aurata) growth, nutrient utilization efficiency, rates of gastric evacuation and digestive enzyme activities}, author = {B Venou and M N Alexis and E Fountoulaki and I Nengas and M Apostolopoulou and I Castritsi-Cathariou}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848603002904}, doi = {10.1016/S0044-8486(03)00290-4}, issn = {00448486}, year = {2003}, date = {2003-07-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {225}, number = {1-4}, pages = {207--223}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Fountoulaki, E; Alexis, M N; Nengas, I; Venou, B Aquaculture, 225 (1-4), pp. 309–323, 2003, ISSN: 00448486. @article{fountoulaki_effects_2003, title = {Effects of dietary arachidonic acid (20:4n-6), on growth, body composition, and tissue fatty acid profile of gilthead bream fingerlings (Sparus aurata L.)}, author = {E Fountoulaki and M N Alexis and I Nengas and B Venou}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848603002989}, doi = {10.1016/S0044-8486(03)00298-9}, issn = {00448486}, year = {2003}, date = {2003-07-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {225}, number = {1-4}, pages = {309--323}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Grigorakis, K; Taylor, K D A; Alexis, M N Aquaculture, 225 (1-4), pp. 109–119, 2003, ISSN: 00448486. @article{grigorakis_organoleptic_2003, title = {Organoleptic and volatile aroma compounds comparison of wild and cultured gilthead sea bream (Sparus aurata): sensory differences and possible chemical basis}, author = {K Grigorakis and K D A Taylor and M N Alexis}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848603002837}, doi = {10.1016/S0044-8486(03)00283-7}, issn = {00448486}, year = {2003}, date = {2003-07-01}, urldate = {2020-08-10}, journal = {Aquaculture}, volume = {225}, number = {1-4}, pages = {109--119}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Probst, H C; Lagnel, J; Kollias, G; van den Broek, M Inducible transgenic mice reveal resting dendritic cells as potent inducers of CD8(+) Ŧ cell tolerance Journal Article Immunity, 18 (5), pp. 713–720, 2003, (ISBN: 1074-7613). @article{probst_inducible_2003, title = {Inducible transgenic mice reveal resting dendritic cells as potent inducers of CD8(+) Ŧ cell tolerance}, author = {H C Probst and J Lagnel and G Kollias and M van den Broek}, url = {<Go to ISI>://000182962200015}, year = {2003}, date = {2003-05-01}, journal = {Immunity}, volume = {18}, number = {5}, pages = {713--720}, abstract = {Dendritic cells (DC) are inducers of immune responses par excellence. They also seem responsible for the induction of peripheral T cell tolerance. To investigate these opposite functions of DC, we generated a Cre/LoxP-based system that allows inducible antigen presentation by DC in vivo. This enables us to study the immunogical consequences of antigen presentation by resting versus mature DC without adoptively transferring DC and with physiological numbers of endogenous, naive responder T cells. We found that presentation of LCMV-derived CTL epitopes by resting DC resulted in antigen-specific tolerance, which could not be broken by subsequent infection with LCMV. On the other hand, antigen presentation by activated DC primed endogenous CTL to expand and to develop protective effector function.}, note = {ISBN: 1074-7613}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dendritic cells (DC) are inducers of immune responses par excellence. They also seem responsible for the induction of peripheral T cell tolerance. To investigate these opposite functions of DC, we generated a Cre/LoxP-based system that allows inducible antigen presentation by DC in vivo. This enables us to study the immunogical consequences of antigen presentation by resting versus mature DC without adoptively transferring DC and with physiological numbers of endogenous, naive responder T cells. We found that presentation of LCMV-derived CTL epitopes by resting DC resulted in antigen-specific tolerance, which could not be broken by subsequent infection with LCMV. On the other hand, antigen presentation by activated DC primed endogenous CTL to expand and to develop protective effector function. |
Grigorakis, K; Taylor, K D A; Alexis, M N Seasonal patterns of spoilage of ice-stored cultured gilthead sea bream (Sparus aurata) Journal Article Food Chemistry, 81 (2), pp. 263–268, 2003, ISSN: 03088146. @article{grigorakis_seasonal_2003, title = {Seasonal patterns of spoilage of ice-stored cultured gilthead sea bream (Sparus aurata)}, author = {K Grigorakis and K D A Taylor and M N Alexis}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0308814602004211}, doi = {10.1016/S0308-8146(02)00421-1}, issn = {03088146}, year = {2003}, date = {2003-05-01}, urldate = {2020-08-10}, journal = {Food Chemistry}, volume = {81}, number = {2}, pages = {263--268}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Tyrpenou, A E; Kotzamanis, Y P; Alexis, M N Aquaculture, 220 (1-4), pp. 633–642, 2003, ISSN: 00448486. @article{tyrpenou_flumequine_2003, title = {Flumequine depletion from muscle plus skin tissue of gilthead seabream (Sparus aurata L.) fed flumequine medicated feed in seawater at 18 and 24 °C}, author = {A E Tyrpenou and Y P Kotzamanis and M N Alexis}, url = {https://linkinghub.elsevier.com/retrieve/pii/S004484860200251X}, doi = {10.1016/S0044-8486(02)00251-X}, issn = {00448486}, year = {2003}, date = {2003-04-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {220}, number = {1-4}, pages = {633--642}, abstract = {We examined flumequine depletion from muscle plus skin of gilthead seabream held in seawater at 18 and 24°C. Seven groups of 10 fish each were sampled at intervals ranging from 24 to 168 h after in-feed administration of flumequine at 35 mg/kg/day for 5 days. Muscle plus skin tissue samples were analyzed for flumequine by high-performance liquid chromatography and fluorescence detection (HPLC-SFD). Parent flumequine concentrations declined rapidly from muscle plus skin after dosing with elimination half-lives of t1/2=22.14 and 21.43 h at 18 and 24°C, respectively. Withdrawal periods for the maximum residue limit (MRL) of 600 μg/kg flumequine in muscle plus skin at 95% tolerance limit were 106.08 and 75.84 h at 18 and 24°C, respectively, after treatment. © 2003 Elsevier Science B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We examined flumequine depletion from muscle plus skin of gilthead seabream held in seawater at 18 and 24°C. Seven groups of 10 fish each were sampled at intervals ranging from 24 to 168 h after in-feed administration of flumequine at 35 mg/kg/day for 5 days. Muscle plus skin tissue samples were analyzed for flumequine by high-performance liquid chromatography and fluorescence detection (HPLC-SFD). Parent flumequine concentrations declined rapidly from muscle plus skin after dosing with elimination half-lives of t1/2=22.14 and 21.43 h at 18 and 24°C, respectively. Withdrawal periods for the maximum residue limit (MRL) of 600 μg/kg flumequine in muscle plus skin at 95% tolerance limit were 106.08 and 75.84 h at 18 and 24°C, respectively, after treatment. © 2003 Elsevier Science B.V. All rights reserved. |
Sfakianakis, D G; Koumoundouros, G; Anezaki, L; Divanach, P; Kentouri, M Development of a saddleback-like syndrome in reared white seabream Diplodus sargus (Linnaeus, 1758) Journal Article Aquaculture, 217 (1-4), pp. 673–676, 2003, ISSN: 00448486. @article{sfakianakis_development_2003, title = {Development of a saddleback-like syndrome in reared white seabream Diplodus sargus (Linnaeus, 1758)}, author = {D G Sfakianakis and G Koumoundouros and L Anezaki and P Divanach and M Kentouri}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848602001990}, doi = {10.1016/S0044-8486(02)00199-0}, issn = {00448486}, year = {2003}, date = {2003-03-01}, urldate = {2020-08-17}, journal = {Aquaculture}, volume = {217}, number = {1-4}, pages = {673--676}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Koulouri, P T; Dounas, C G; Eleftheriou, A S A new apparatus for the direct measurement of otter trawling effects on the epibenthic and hyperbenthic macrofauna Journal Article Journal of the Marine Biological Association of the United Kingdom, 83 (6), pp. 1363–1368, 2003, ISSN: 00253154. @article{koulouri_new_2003, title = {A new apparatus for the direct measurement of otter trawling effects on the epibenthic and hyperbenthic macrofauna}, author = {P T Koulouri and C G Dounas and A S Eleftheriou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-1042267134&doi=10.1017%2fS0025315403008828&partnerID=40&md5=1086ff028266b8fe562a6160f8f82091}, doi = {10.1017/S0025315403008828}, issn = {00253154}, year = {2003}, date = {2003-01-01}, journal = {Journal of the Marine Biological Association of the United Kingdom}, volume = {83}, number = {6}, pages = {1363--1368}, abstract = {A towed trawl simulator sledge (TTSS2) for collecting quantitatively small macrobenthic animals disturbed by the passage of otter trawl groundrope typical of the local fishery is described and illustrated. The TTSS2 was towed from a surface vessel at a speed within the range of commercial trawls, incorporating three sampling nets that open and close by means of an electro-mechanical system. An odometer in contact with the seabed provided a continuous record of the trawled ground. The degree of efficiency of the TTSS2 - by means of the attached groundrope that stirs up the surface of the bottom ahead of the nets -was tested in the northern continental shelf of Crete (eastern Mediterranean). Additional trials with a much heavier groundropc were also performed. Analysis and comparison of TTSS2 samples with endobenthic, hyperbenthic and planktonic samples collected by means of conventional sampling gears indicate the presence of a well-defined and distinct fauna in the plume of resuspended sediment behind the groundrope.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A towed trawl simulator sledge (TTSS2) for collecting quantitatively small macrobenthic animals disturbed by the passage of otter trawl groundrope typical of the local fishery is described and illustrated. The TTSS2 was towed from a surface vessel at a speed within the range of commercial trawls, incorporating three sampling nets that open and close by means of an electro-mechanical system. An odometer in contact with the seabed provided a continuous record of the trawled ground. The degree of efficiency of the TTSS2 - by means of the attached groundrope that stirs up the surface of the bottom ahead of the nets -was tested in the northern continental shelf of Crete (eastern Mediterranean). Additional trials with a much heavier groundropc were also performed. Analysis and comparison of TTSS2 samples with endobenthic, hyperbenthic and planktonic samples collected by means of conventional sampling gears indicate the presence of a well-defined and distinct fauna in the plume of resuspended sediment behind the groundrope. |
Koutandos, E A numerical and experimental study on the hydrodynamics of a heave motion floating breakwater Inproceedings 2003, (Publication Title: XXX IAHR Congress). @inproceedings{koutandos_numerical_2003, title = {A numerical and experimental study on the hydrodynamics of a heave motion floating breakwater}, author = {E Koutandos}, year = {2003}, date = {2003-01-01}, volume = {JFK competition}, note = {Publication Title: XXX IAHR Congress}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Plaiti, W; Tselepides, A Amphipod community structure in an area of intensive natural and antropogenic disturbance (Termaicos Bay in the Mediteranean Sea). Inproceedings Tunis, Tunisia, 2003, (Publication Title: The11th International Colloquium on Amphipoda, Type: Abstract). @inproceedings{plaiti_amphipod_2003, title = {Amphipod community structure in an area of intensive natural and antropogenic disturbance (Termaicos Bay in the Mediteranean Sea).}, author = {W Plaiti and A Tselepides}, year = {2003}, date = {2003-01-01}, address = {Tunis, Tunisia}, note = {Publication Title: The11th International Colloquium on Amphipoda, Type: Abstract}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Iliopoulou-Georgudaki, J; Kantzaris, V; Katharios, P; Kaspiris, P; Georgiadis, Th.; Montesantou, B An application of different bioindicators for assessing water quality: A case study in the rivers Alfeios and Pineios (Peloponnisos, Greece) Journal Article Ecological Indicators, 2 (4), pp. 345–360, 2003, ISSN: 1470160X, (Publisher: Elsevier). @article{iliopoulou-georgudaki_application_2003, title = {An application of different bioindicators for assessing water quality: A case study in the rivers Alfeios and Pineios (Peloponnisos, Greece)}, author = {J Iliopoulou-Georgudaki and V Kantzaris and P Katharios and P Kaspiris and Th. Georgiadis and B Montesantou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0141993249&doi=10.1016%2fS1470-160X%2803%2900004-9&partnerID=40&md5=0655df035f5bd0c4f216644b1fe3c623}, doi = {10.1016/S1470-160X(03)00004-9}, issn = {1470160X}, year = {2003}, date = {2003-01-01}, journal = {Ecological Indicators}, volume = {2}, number = {4}, pages = {345--360}, abstract = {A number of bioindicators and biotic indices and scores based on benthic macroinvertebrates, diatoms, fishes, aquatic and riparian vegetation in relation to physicochemical parameters have been applied in assessing the water quality of the rivers Alfeios and Pineios (Peloponnisos, Greece). According to the findings, the water quality in both rivers varied from very poor to very good. Among the bioindicators used, the benthic macroinvertebrates seem to be the most reliable. The BBI and IBE were the most applicable indexes while the applicability of the IBMWP and IASPT in the Greek region can be enforced with the inclusion of the Diptreran family Rhagionidae and the Coleopteran family Elminthidae. © 2003 Published by Elsevier Science Ltd.}, note = {Publisher: Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {article} } A number of bioindicators and biotic indices and scores based on benthic macroinvertebrates, diatoms, fishes, aquatic and riparian vegetation in relation to physicochemical parameters have been applied in assessing the water quality of the rivers Alfeios and Pineios (Peloponnisos, Greece). According to the findings, the water quality in both rivers varied from very poor to very good. Among the bioindicators used, the benthic macroinvertebrates seem to be the most reliable. The BBI and IBE were the most applicable indexes while the applicability of the IBMWP and IASPT in the Greek region can be enforced with the inclusion of the Diptreran family Rhagionidae and the Coleopteran family Elminthidae. © 2003 Published by Elsevier Science Ltd. |
Karambas, T; Koutandos, E; Karathanasi, E Boussinesq modeling of the alongshore sediment transport Inproceedings 2003, (Publication Title: XXX IAHR Congress). @inproceedings{karambas_boussinesq_2003, title = {Boussinesq modeling of the alongshore sediment transport}, author = {T Karambas and E Koutandos and E Karathanasi}, year = {2003}, date = {2003-01-01}, volume = {Α}, note = {Publication Title: XXX IAHR Congress}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Mandalakis, M; Berresheim, H; Stephanou, E G Direct evidence for destruction of polychlorobiphenyls by OH radicals in the subtropical troposphere Journal Article Environmental Science and Technology, 37 (3), pp. 542–547, 2003, ISSN: 0013936X. @article{mandalakis_direct_2003, title = {Direct evidence for destruction of polychlorobiphenyls by OH radicals in the subtropical troposphere}, author = {M Mandalakis and H Berresheim and E G Stephanou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0346668318&doi=10.1021%2fes020163i&partnerID=40&md5=cc89166d5dfc3f872f49f7a04a15e1bf}, doi = {10.1021/es020163i}, issn = {0013936X}, year = {2003}, date = {2003-01-01}, journal = {Environmental Science and Technology}, volume = {37}, number = {3}, pages = {542--547}, abstract = {Although laboratory studies have indicated that OH radicals readily oxidize polychorobiphenyls (PCBs) in the gas-phase, this mechanism has never been positively demonstrated under real atmospheric conditions. By applying elaborated sampling and analytical techniques we achieved for the first time simultaneously a field study of the diurnal atmospheric cycle of OH radical and PCBs in a remote site of eastern Mediterranean. In all cases, the concentration of ΣPCB (sum of 27 congeners) showed a characteristic depletion during daytime, while the concentration of OH radicals was at the maximum levels. By assuming that the depletion of PCBs was caused solely by the destruction from OH radicals, PCB-OH rate constants (KOH) of different PCB congeners were determined from field data by applying the relative rate method. Our field-determined KOH values were notably coherent with those previously measured in laboratory experiments. In all measurement periods, the KOH values consistently decreased in sequence for those compounds showing an increasing degree of chlorination on the biphenyl group. Bytaking into account KOH values and latitude-dependent concentration of OH radicals, it was estimated that, near to tropical and subtropical regions, the atmospheric lifetimes of PCBs 8 and 110 should be substantially low (10 and 20 days, respectively). A significant fraction of PCBs should be destroyed during their residence over tropical/subtropical regions, due to the intensive destruction by OH.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Although laboratory studies have indicated that OH radicals readily oxidize polychorobiphenyls (PCBs) in the gas-phase, this mechanism has never been positively demonstrated under real atmospheric conditions. By applying elaborated sampling and analytical techniques we achieved for the first time simultaneously a field study of the diurnal atmospheric cycle of OH radical and PCBs in a remote site of eastern Mediterranean. In all cases, the concentration of ΣPCB (sum of 27 congeners) showed a characteristic depletion during daytime, while the concentration of OH radicals was at the maximum levels. By assuming that the depletion of PCBs was caused solely by the destruction from OH radicals, PCB-OH rate constants (KOH) of different PCB congeners were determined from field data by applying the relative rate method. Our field-determined KOH values were notably coherent with those previously measured in laboratory experiments. In all measurement periods, the KOH values consistently decreased in sequence for those compounds showing an increasing degree of chlorination on the biphenyl group. Bytaking into account KOH values and latitude-dependent concentration of OH radicals, it was estimated that, near to tropical and subtropical regions, the atmospheric lifetimes of PCBs 8 and 110 should be substantially low (10 and 20 days, respectively). A significant fraction of PCBs should be destroyed during their residence over tropical/subtropical regions, due to the intensive destruction by OH. |
Kotoulas, G; Mejuto, J; Tserpes, G; Garcia-Cortes, B; Peristeraki, P; la Serna, De J M; Magoulas, A DNA microsatellite markers in service of swordfish stock-structure analysis in the Atlantic and Mediterranean Journal Article ICCAT Col. Vol. Sci. Papers, 55 (4), pp. 1632–1639, 2003. @article{kotoulas_dna_2003, title = {DNA microsatellite markers in service of swordfish stock-structure analysis in the Atlantic and Mediterranean}, author = {G Kotoulas and J Mejuto and G Tserpes and B Garcia-Cortes and P Peristeraki and J M De la Serna and A Magoulas}, year = {2003}, date = {2003-01-01}, journal = {ICCAT Col. Vol. Sci. Papers}, volume = {55}, number = {4}, pages = {1632--1639}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Polymenakou, P N; Hatziyanni, E; Tselepides, A; Stephanou, E G Effect of incubation experiments on the distribution of different lipid classes (aliphatic hydrocarbons, diglycerides, free fatty acids, glycolipids, phospholipids) in sediments of the Cretan Sea Inproceedings Hersonissos, Crete, Greece, 2003, (Publication Title: 7th Symposium of Oceanography & Fisheries Type: Poster). @inproceedings{polymenakou_effect_2003, title = {Effect of incubation experiments on the distribution of different lipid classes (aliphatic hydrocarbons, diglycerides, free fatty acids, glycolipids, phospholipids) in sediments of the Cretan Sea}, author = {P N Polymenakou and E Hatziyanni and A Tselepides and E G Stephanou}, year = {2003}, date = {2003-01-01}, address = {Hersonissos, Crete, Greece}, note = {Publication Title: 7th Symposium of Oceanography & Fisheries Type: Poster}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Tsigenopoulos, C S; Hellemans, B; Chistiakov, D A; Libertini, A; Kotoulas, G; Volckaert, F Eleven new microsatellites of the sea bass (Dicentrarchus labrax L.) Journal Article Molecular Ecology Notes, 3 (3), pp. 352–354, 2003, ISSN: 14718278. @article{tsigenopoulos_eleven_2003, title = {Eleven new microsatellites of the sea bass (Dicentrarchus labrax L.)}, author = {C S Tsigenopoulos and B Hellemans and D A Chistiakov and A Libertini and G Kotoulas and F Volckaert}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0041874880&doi=10.1046%2fj.1471-8286.2003.00447.x&partnerID=40&md5=821b63e875e1041a9f4dd3f9e973c928}, doi = {10.1046/j.1471-8286.2003.00447.x}, issn = {14718278}, year = {2003}, date = {2003-01-01}, journal = {Molecular Ecology Notes}, volume = {3}, number = {3}, pages = {352--354}, abstract = {Eleven polymorphic microsatellites were isolated from the sea bass, Dicentrarchus labrax, using a microsatellite enrichment protocol and selective hybridization with an (AC)12 probe. The loci showed different variation patterns in 21 unrelated sea bass individuals, with a mean number of alleles of 8.6 and a mean observed heterozygosity of 0.68. These microsatellite markers should be useful for population genetic analysis and biodiversity studies of sea bass.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Eleven polymorphic microsatellites were isolated from the sea bass, Dicentrarchus labrax, using a microsatellite enrichment protocol and selective hybridization with an (AC)12 probe. The loci showed different variation patterns in 21 unrelated sea bass individuals, with a mean number of alleles of 8.6 and a mean observed heterozygosity of 0.68. These microsatellite markers should be useful for population genetic analysis and biodiversity studies of sea bass. |
Rainis, S; Mylonas, C C; Kyriakou, Y; Divanach, P Enhancement of spermiation in European sea bass (Dicentrarchus labrax) at the end of the reproductive season using GnRHa implants Journal Article Aquaculture, 219 (1-4), pp. 873–890, 2003, ISSN: 00448486, (Publisher: Elsevier). @article{rainis_enhancement_2003, title = {Enhancement of spermiation in European sea bass (Dicentrarchus labrax) at the end of the reproductive season using GnRHa implants}, author = {S Rainis and C C Mylonas and Y Kyriakou and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037414059&doi=10.1016%2fS0044-8486%2803%2900028-0&partnerID=40&md5=1ef1061621cd17c6bab8bac850349250}, doi = {10.1016/S0044-8486(03)00028-0}, issn = {00448486}, year = {2003}, date = {2003-01-01}, journal = {Aquaculture}, volume = {219}, number = {1-4}, pages = {873--890}, abstract = {Spermiating European sea bass (Dicentrarchus labrax) were treated at the end of the reproductive period (22 February 2002) with implants of gonadotropin-releasing hormone agonist (GnRHa), in order to examine their potential in enhancing expressible milt volume, sperm quality and in vitro fertilization success. The GnRHa implants at all doses (20, 40 and 80 μg GnRHa kg-1 body weight [bw]) stimulated a significantly higher production of milt compared to controls over a 27-day period, increasing total volume from 0.5 to 2.2 ml kg-1 sample time-1. No differences in milt production were observed among the three GnRHa doses. Histological evaluation of the testes indicated that, by day 21, control fish had entered into gonadal regression, containing almost exclusively spermatogonia. At the same time, testes from GnRHa-implanted fish still contained large numbers of spermatozoa in the testes, indicating that spermiation was still underway. Sperm density varied between 7.4 and 14.4 × 1010 spermatozoa ml-1, but remained unchanged in response to GnRHa implantation. Sperm motility was high at the beginning of the experiment (92-100%) and decreased significantly by day 27 (32-55%), whereas motility duration was unchanged (0.9-1.2 min). Neither sperm motility nor motility duration was affected by the GnRHa treatment. Finally, fertilization success was high (40-80%) and not significantly different in response to GnRHa implantation. These results demonstrate that GnRHa implants are effective at increasing milt production at the end of the spermiation period, maintaining high sperm density and not affecting, either positively or negatively, sperm quality and fertilization capacity. © 2003 Elsevier Science B.V. All rights reserved.}, note = {Publisher: Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {article} } Spermiating European sea bass (Dicentrarchus labrax) were treated at the end of the reproductive period (22 February 2002) with implants of gonadotropin-releasing hormone agonist (GnRHa), in order to examine their potential in enhancing expressible milt volume, sperm quality and in vitro fertilization success. The GnRHa implants at all doses (20, 40 and 80 μg GnRHa kg-1 body weight [bw]) stimulated a significantly higher production of milt compared to controls over a 27-day period, increasing total volume from 0.5 to 2.2 ml kg-1 sample time-1. No differences in milt production were observed among the three GnRHa doses. Histological evaluation of the testes indicated that, by day 21, control fish had entered into gonadal regression, containing almost exclusively spermatogonia. At the same time, testes from GnRHa-implanted fish still contained large numbers of spermatozoa in the testes, indicating that spermiation was still underway. Sperm density varied between 7.4 and 14.4 × 1010 spermatozoa ml-1, but remained unchanged in response to GnRHa implantation. Sperm motility was high at the beginning of the experiment (92-100%) and decreased significantly by day 27 (32-55%), whereas motility duration was unchanged (0.9-1.2 min). Neither sperm motility nor motility duration was affected by the GnRHa treatment. Finally, fertilization success was high (40-80%) and not significantly different in response to GnRHa implantation. These results demonstrate that GnRHa implants are effective at increasing milt production at the end of the spermiation period, maintaining high sperm density and not affecting, either positively or negatively, sperm quality and fertilization capacity. © 2003 Elsevier Science B.V. All rights reserved. |
Pavlidis, M; Angellotti, L; Papandroulakis, N; Divanach, P Evaluation of transportation procedures on water quality and fry performance in red porgy (Pagrus pagrus) fry Journal Article Aquaculture, 218 , pp. 187 – 202, 2003. @article{pavlidis_evaluation_2003, title = {Evaluation of transportation procedures on water quality and fry performance in red porgy (Pagrus pagrus) fry}, author = {M Pavlidis and L Angellotti and N Papandroulakis and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0037468983&partnerID=40&md5=80ecd722fd5c0e27d85db4c0de5ae08f}, year = {2003}, date = {2003-01-01}, journal = {Aquaculture}, volume = {218}, pages = {187 -- 202}, abstract = {The effect of stocking density (10, 20, 30, 40 kg/m3), water renewal (0% or 100% renewal rate per hour), hauling temperature (14, 19, 24 °C), salinity (20, 25, 30, 35, 40 psu), and use of anaesthesia (0, 10, 20, or 50 ppm ethynelglycol-monophenylether) prior to transportation on red porgy’s fry performance, liver glycogen, and water quality parameters was tested to evaluate transportation procedures in a promising candidate for aquaculture. Simulated transport was performed in plastic containers (volume 60 1) for 48 h. Water samples were taken at 4-h intervals after transport for the determination of pH, carbon dioxide (CO2), un-ionised ammonia (NH3) and ammonium (NH4 +). Additionally, liver samples were collected at 4 and 48 h for glycogen determination. There were no statistically significant fluctuations in dissolved CO2 concentration in all tested conditions. Stocking density did not affect NH3 and NH4 + average values and hepatic glycogen content in groups exposed to a water renewal rate of 100%, while increasing NH3 and NH4 + average values with increasing stocking density was observed in groups with no water renewal. Under the same stocking density, a significant change in NH3 and NH4 + fluctuations over the duration of the experiment was observed with concentrations increasing, with a mean exponential rate (± SD) of 0.060 ± 0.005 (NH3) and 0.062 ± 0.005 (NH4 +) per hour in groups with no water renewal, and -0.033 ± 0.004 (NH3) and -0.024 ± 0.007 (NH4 +) per hour in groups with 100% water renewal. Water temperature affected significantly the hepatic glycogen content and survival during transport. There was no significant effect of salinity and anaesthetic (except at a dose of 50 ppm) on fry survival and on the water quality parameters. It is suggested that red porgy should be transported in stocking densities of 20-25 kg/m3 and at a hauling temperature similar to that kept at the exporter’s fish rearing tanks (preferable 19 °C). Besides, it is recommended to avoid temperature differences between the hauling water and the water used for renewal during fry transportation. © 2003 Elsevier Science B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The effect of stocking density (10, 20, 30, 40 kg/m3), water renewal (0% or 100% renewal rate per hour), hauling temperature (14, 19, 24 °C), salinity (20, 25, 30, 35, 40 psu), and use of anaesthesia (0, 10, 20, or 50 ppm ethynelglycol-monophenylether) prior to transportation on red porgy’s fry performance, liver glycogen, and water quality parameters was tested to evaluate transportation procedures in a promising candidate for aquaculture. Simulated transport was performed in plastic containers (volume 60 1) for 48 h. Water samples were taken at 4-h intervals after transport for the determination of pH, carbon dioxide (CO2), un-ionised ammonia (NH3) and ammonium (NH4 +). Additionally, liver samples were collected at 4 and 48 h for glycogen determination. There were no statistically significant fluctuations in dissolved CO2 concentration in all tested conditions. Stocking density did not affect NH3 and NH4 + average values and hepatic glycogen content in groups exposed to a water renewal rate of 100%, while increasing NH3 and NH4 + average values with increasing stocking density was observed in groups with no water renewal. Under the same stocking density, a significant change in NH3 and NH4 + fluctuations over the duration of the experiment was observed with concentrations increasing, with a mean exponential rate (± SD) of 0.060 ± 0.005 (NH3) and 0.062 ± 0.005 (NH4 +) per hour in groups with no water renewal, and -0.033 ± 0.004 (NH3) and -0.024 ± 0.007 (NH4 +) per hour in groups with 100% water renewal. Water temperature affected significantly the hepatic glycogen content and survival during transport. There was no significant effect of salinity and anaesthetic (except at a dose of 50 ppm) on fry survival and on the water quality parameters. It is suggested that red porgy should be transported in stocking densities of 20-25 kg/m3 and at a hauling temperature similar to that kept at the exporter’s fish rearing tanks (preferable 19 °C). Besides, it is recommended to avoid temperature differences between the hauling water and the water used for renewal during fry transportation. © 2003 Elsevier Science B.V. All rights reserved. |
Koutandos, E; Prinos, P; Gironella, X Floating breakwaters interaction with waves-A large scale experimental study Inproceedings pp. 385–392, 2003, (Publication Title: XXX IAHR Congress). @inproceedings{koutandos_floating_2003, title = {Floating breakwaters interaction with waves-A large scale experimental study}, author = {E Koutandos and P Prinos and X Gironella}, year = {2003}, date = {2003-01-01}, volume = {A}, pages = {385--392}, note = {Publication Title: XXX IAHR Congress}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Rodríguez, L; Halm, S; Mylonas, C; Zanuy, S Gene expression of estrogen receptors α and β during early sexual differentiation in the European sea bass (Dicentrarchus labrax) Journal Article Fish Physiology and Biochemistry, 28 (1-4), pp. 175, 2003, ISSN: 09201742. @article{rodriguez_gene_2003, title = {Gene expression of estrogen receptors α and β during early sexual differentiation in the European sea bass (Dicentrarchus labrax)}, author = {L Rodríguez and S Halm and C Mylonas and S Zanuy}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-8844240623&doi=10.1023%2fB%3aFISH.0000030518.51999.5d&partnerID=40&md5=b4beeb4933cde8912c17a9b6d0ee357b}, doi = {10.1023/B:FISH.0000030518.51999.5d}, issn = {09201742}, year = {2003}, date = {2003-01-01}, journal = {Fish Physiology and Biochemistry}, volume = {28}, number = {1-4}, pages = {175}, abstract = {A dimorphic expression pattern of ERα was found during sexual development in the European sea bass. It is therefore suggested that ERα plays an important role in sexual differentiation in this species. © 2004 Kluwer Academic Publishers.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A dimorphic expression pattern of ERα was found during sexual development in the European sea bass. It is therefore suggested that ERα plays an important role in sexual differentiation in this species. © 2004 Kluwer Academic Publishers. |
Mandalakis, M; Stephanou, E G Chimia, 57 (9), pp. 505–508, 2003, ISSN: 00094293, (Publisher: Swiss Chemical Society). @article{mandalakis_high-resolution_2003, title = {High-resolution gas chromatographytandem mass spectrometry: A sensitive analytical technique suitable for the study of atmospheric processes of polychlorinated biphenyls and dibenzo-p-dioxins/furans}, author = {M Mandalakis and E G Stephanou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0142120836&doi=10.2533%2f000942903777678984&partnerID=40&md5=4b93582001ea9a5083275dafbcce3443}, doi = {10.2533/000942903777678984}, issn = {00094293}, year = {2003}, date = {2003-01-01}, journal = {Chimia}, volume = {57}, number = {9}, pages = {505--508}, abstract = {The trace-level environmental determination of polychlorinated biphenyls (PCBs) and 2,3,7,8-substituted polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) requires the use of delicate fractionation and analytical techniques such as high-resolution gas chromatography coupled with high-resolution mass spectrometry. The analytical requirements are even higher if atmospheric physicochemical processes of these persistent organic compounds (POPs) are to be studied in remote areas, where concentrations are very low. The optimization of a fractionation method, and of the high-resolution gas chromatography/ion trap tandem mass spectrometry system instrumental parameters enabled us to establish the optimum conditions for the determination of atmospheric PCBs and PCDD/Fs at the low pg or even fg m-3 level. By using this analytical procedure, we accomplished the reliable determination of ca. 54 PCBs and 17 PCDD/Fs individual congeners, of different chlorination level, in the gas and particulate phase of atmospheric aerosol samples collected from the Eastern Mediterranean basin. Furthermore, we were also able to study the atmospheric physicochemical and loss processes of PCBs, such as gas-particle partitioning, wet and dry deposition, and PCBs reaction with hydroxyl radicals.}, note = {Publisher: Swiss Chemical Society}, keywords = {}, pubstate = {published}, tppubtype = {article} } The trace-level environmental determination of polychlorinated biphenyls (PCBs) and 2,3,7,8-substituted polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) requires the use of delicate fractionation and analytical techniques such as high-resolution gas chromatography coupled with high-resolution mass spectrometry. The analytical requirements are even higher if atmospheric physicochemical processes of these persistent organic compounds (POPs) are to be studied in remote areas, where concentrations are very low. The optimization of a fractionation method, and of the high-resolution gas chromatography/ion trap tandem mass spectrometry system instrumental parameters enabled us to establish the optimum conditions for the determination of atmospheric PCBs and PCDD/Fs at the low pg or even fg m-3 level. By using this analytical procedure, we accomplished the reliable determination of ca. 54 PCBs and 17 PCDD/Fs individual congeners, of different chlorination level, in the gas and particulate phase of atmospheric aerosol samples collected from the Eastern Mediterranean basin. Furthermore, we were also able to study the atmospheric physicochemical and loss processes of PCBs, such as gas-particle partitioning, wet and dry deposition, and PCBs reaction with hydroxyl radicals. |
Marino, G; Panini, E; Longobardi, A; Mandich, A; Finoia, M G; Zohar, Y; Mylonas, C C Induction of ovulation in captive-reared dusky grouper, Epinephelus marginatus (Lowe, 1834), with a sustained-release GnRHa implant Journal Article Aquaculture, 219 (1-4), pp. 841–858, 2003, ISSN: 00448486, (Publisher: Elsevier). @article{marino_induction_2003, title = {Induction of ovulation in captive-reared dusky grouper, Epinephelus marginatus (Lowe, 1834), with a sustained-release GnRHa implant}, author = {G Marino and E Panini and A Longobardi and A Mandich and M G Finoia and Y Zohar and C C Mylonas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037414001&doi=10.1016%2fS0044-8486%2803%2900036-X&partnerID=40&md5=60178ae27c4bbe6be911ce3d5e69ed00}, doi = {10.1016/S0044-8486(03)00036-X}, issn = {00448486}, year = {2003}, date = {2003-01-01}, journal = {Aquaculture}, volume = {219}, number = {1-4}, pages = {841--858}, abstract = {Captive-reared dusky grouper were induced to ovulate using a sustained-release delivery system (implant) loaded with gonadotropin-releasing hormone agonist [D-Ala6, Pro9, NEt]-GnRH (GnRHa). Thirteen females were implanted at doses ranging from 30.5 to 68.3 μg kg-1 during three experiments between late June and early September. Of the injected females, 85% responded positively to the GnRHa implant and ovulated between 60 and 238 h after treatment, whereas none of control fish showed any sign of maturation. No spontaneous spawning was observed, and the eggs were manually removed from the females using gentle abdominal pressure. The mean number of ovulations per fish was 3.8, with a maximum of nine for one female. Overall, a total of 42 ovulations were obtained, resulting in the production of more than 5 million eggs. The average relative fecundity was 118.3 ± 16.0 × 103 eggs kg-1 BW, with a maximum of 202.2 × 103 eggs kg-1 BW. Mean percentage fertilisation and hatching were 48.2% and 52.2%, respectively. The results demonstrate that GnRHa administration via controlled delivery systems is an effective method for producing good quality eggs in captive dusky grouper. © 2003 Elsevier Science B.V. All rights reserved.}, note = {Publisher: Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {article} } Captive-reared dusky grouper were induced to ovulate using a sustained-release delivery system (implant) loaded with gonadotropin-releasing hormone agonist [D-Ala6, Pro9, NEt]-GnRH (GnRHa). Thirteen females were implanted at doses ranging from 30.5 to 68.3 μg kg-1 during three experiments between late June and early September. Of the injected females, 85% responded positively to the GnRHa implant and ovulated between 60 and 238 h after treatment, whereas none of control fish showed any sign of maturation. No spontaneous spawning was observed, and the eggs were manually removed from the females using gentle abdominal pressure. The mean number of ovulations per fish was 3.8, with a maximum of nine for one female. Overall, a total of 42 ovulations were obtained, resulting in the production of more than 5 million eggs. The average relative fecundity was 118.3 ± 16.0 × 103 eggs kg-1 BW, with a maximum of 202.2 × 103 eggs kg-1 BW. Mean percentage fertilisation and hatching were 48.2% and 52.2%, respectively. The results demonstrate that GnRHa administration via controlled delivery systems is an effective method for producing good quality eggs in captive dusky grouper. © 2003 Elsevier Science B.V. All rights reserved. |
Mylonas, C C; Anezaki, L; Divanach, P; Zanuy, S; Piferrer, F; Ron, B; Peduel, A; Atia, Ben I; Gorshkov, S; Tandler, A Influence of rearing temperature at two periods during early life on growth and sex differentiation of two strains of European sea bass Journal Article Fish Physiology and Biochemistry, 28 (1-4), pp. 167–168, 2003, ISSN: 09201742. @article{mylonas_influence_2003, title = {Influence of rearing temperature at two periods during early life on growth and sex differentiation of two strains of European sea bass}, author = {C C Mylonas and L Anezaki and P Divanach and S Zanuy and F Piferrer and B Ron and A Peduel and I Ben Atia and S Gorshkov and A Tandler}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-8844247073&doi=10.1023%2fB%3aFISH.0000030514.63577.84&partnerID=40&md5=14276c977f32f39ea79feff0f756a6c1}, doi = {10.1023/B:FISH.0000030514.63577.84}, issn = {09201742}, year = {2003}, date = {2003-01-01}, journal = {Fish Physiology and Biochemistry}, volume = {28}, number = {1-4}, pages = {167--168}, abstract = {The effect of water temperature on sex differentiation was examined in European sea bass (Dicentrarchus labrax) of two strains from the Northwestern and Southeastern Mediterranean Sea, during larval or nursery rearing. Temperatures textless 17 °C enhanced feminization, while 21 °C during larval rearing induced higher male% compared to the nursery stage, and the overall male% was significantly different among strains (Ptextless0.05). The study shows that management of larval rearing temperature can prevent the high male% observed in cultured populations. © 2004 Kluwer Academic Publishers.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The effect of water temperature on sex differentiation was examined in European sea bass (Dicentrarchus labrax) of two strains from the Northwestern and Southeastern Mediterranean Sea, during larval or nursery rearing. Temperatures textless 17 °C enhanced feminization, while 21 °C during larval rearing induced higher male% compared to the nursery stage, and the overall male% was significantly different among strains (Ptextless0.05). The study shows that management of larval rearing temperature can prevent the high male% observed in cultured populations. © 2004 Kluwer Academic Publishers. |
Mandalakis, M; Stephanou, E G Loss Processes of PCBs in the subtropical atmosphere: Study of PCB-OH radical reactions in eastern Mediterranean Inproceedings Organohalogen Compounds, pp. 2, 2003. @inproceedings{mandalakis_loss_2003, title = {Loss Processes of PCBs in the subtropical atmosphere: Study of PCB-OH radical reactions in eastern Mediterranean}, author = {M Mandalakis and E G Stephanou}, year = {2003}, date = {2003-01-01}, booktitle = {Organohalogen Compounds}, volume = {60-65}, pages = {2}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Poulakakis, N; Lymberakis, P; Antoniou, A; Chalkia, D; Zouros, E; Mylonas, M; Valakos, E Molecular phylogeny and biogeography of the wall-lizard Podarcis erhardii (Squamata: Lacertidae) Journal Article Molecular Phylogenetics and Evolution, 28 , pp. 38–46, 2003. @article{poulakakis_molecular_2003, title = {Molecular phylogeny and biogeography of the wall-lizard Podarcis erhardii (Squamata: Lacertidae)}, author = {N Poulakakis and P Lymberakis and A Antoniou and D Chalkia and E Zouros and M Mylonas and E Valakos}, url = {http://www.sciencedirect.com/science/article/pii/S105579030300037X}, doi = {DOI:.org/10.1016/S1055-7903(03)00037-X}, year = {2003}, date = {2003-01-01}, journal = {Molecular Phylogenetics and Evolution}, volume = {28}, pages = {38--46}, abstract = {Erhard's wall lizard, Podarcis erhardii (Sauria: Lacertidae), is highly diversified in Greece and especially in the southern Aegean region. Out of the 28 recognized subspecies, 27 are found in Greece from the North Sporades island-complex in the North Aegean (grossly south of the 39th parallel) to the island of Crete in the South. The species exhibits great morphological and ecological plasticity and inhabits many different habitats from rocky islets and sandy shores to mountaintops as high as 2000 m. By examining intraspecific variability at a segment of the mitochondrial gene cytochrome b we have found that that extant populations of P. erhardii are paraphyletic. Furthermore, we have found that subspecies previously defined on the basis of morphological characteristics do not correspond to different molecular phylogenetic clades, so that their status should be reconsidered. The DNA based biogeographical and phylogenetic history of Podarcis in Southern Greece is congruent with available paleogeographic data of the region, which supports the view that DNA sequences may be a useful tool for the study of palaeogeography.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Erhard's wall lizard, Podarcis erhardii (Sauria: Lacertidae), is highly diversified in Greece and especially in the southern Aegean region. Out of the 28 recognized subspecies, 27 are found in Greece from the North Sporades island-complex in the North Aegean (grossly south of the 39th parallel) to the island of Crete in the South. The species exhibits great morphological and ecological plasticity and inhabits many different habitats from rocky islets and sandy shores to mountaintops as high as 2000 m. By examining intraspecific variability at a segment of the mitochondrial gene cytochrome b we have found that that extant populations of P. erhardii are paraphyletic. Furthermore, we have found that subspecies previously defined on the basis of morphological characteristics do not correspond to different molecular phylogenetic clades, so that their status should be reconsidered. The DNA based biogeographical and phylogenetic history of Podarcis in Southern Greece is congruent with available paleogeographic data of the region, which supports the view that DNA sequences may be a useful tool for the study of palaeogeography. |
Mylonas, C C; Sigelaki, I; Divanach, P; Mananõs, E; Carrillo, M; Afonso-Polyviou, A Multiple spawning and egg quality of individual European sea bass (Dicentrarchus labrax) females after repeated injections of GnRHa Journal Article Aquaculture, 221 (1-4), pp. 605–620, 2003, ISSN: 00448486, (Publisher: Elsevier). @article{mylonas_multiple_2003, title = {Multiple spawning and egg quality of individual European sea bass (Dicentrarchus labrax) females after repeated injections of GnRHa}, author = {C C Mylonas and I Sigelaki and P Divanach and E Mananõs and M Carrillo and A Afonso-Polyviou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037402489&doi=10.1016%2fS0044-8486%2803%2900120-0&partnerID=40&md5=4fa3ca75d178e099a929643bd3f005cd}, doi = {10.1016/S0044-8486(03)00120-0}, issn = {00448486}, year = {2003}, date = {2003-01-01}, journal = {Aquaculture}, volume = {221}, number = {1-4}, pages = {605--620}, abstract = {Using multiple injections of gonadotropin-releasing hormone agonist (GnRHa) (10 μg kg-1 bw) spaced 7-14 days apart, we investigated the multiple-batch group-synchronous spawning kinetics of individual European sea bass females (n = 6) and examined batch fecundity and egg quality parameters. Spawning was obtained 3-4 days after each GnRHa injection. A mean of 2.8 spawns female-1 was obtained, with a minimum of 2 and maximum of 4. Mean relative fecundity of the first spawn was 320,211 eggs kg-1 bw and decreased continuously in subsequent spawns, reaching 52,381 eggs kg-1. Total mean relative fecundity for all spawns was 637,900 eggs kg-1. Mean fertilization success did not vary significantly among sequential spawns and ranged between 67% and 91%. On the contrary, there were significant differences among the four spawns in embryo survival 1 day after egg collection, hatching percentage and larval survival 4 days after egg collection, but there was no consistent trend during the study. Significant positive correlations existed between 4-day larval survival and hatching percentage (P=0.0002), and between hatching percentage and embryo survival 1 day after egg collection (P=0.03), indicating that 1-day embryo survival can be a potential biomarker for egg quality in commercial hatcheries. The results of the study demonstrate that European sea bass can produce up to four sequential spawns of high quality eggs in response to repeated acute treatments of GnRHa, and suggest that a pulsatile treatment of GnRHa may be necessary to induce the appropriate cycles of hormone changes for the recruitment, maturation and ovulation of multiple batches of viable eggs. © 2003 Elsevier Science B.V. All rights reserved.}, note = {Publisher: Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {article} } Using multiple injections of gonadotropin-releasing hormone agonist (GnRHa) (10 μg kg-1 bw) spaced 7-14 days apart, we investigated the multiple-batch group-synchronous spawning kinetics of individual European sea bass females (n = 6) and examined batch fecundity and egg quality parameters. Spawning was obtained 3-4 days after each GnRHa injection. A mean of 2.8 spawns female-1 was obtained, with a minimum of 2 and maximum of 4. Mean relative fecundity of the first spawn was 320,211 eggs kg-1 bw and decreased continuously in subsequent spawns, reaching 52,381 eggs kg-1. Total mean relative fecundity for all spawns was 637,900 eggs kg-1. Mean fertilization success did not vary significantly among sequential spawns and ranged between 67% and 91%. On the contrary, there were significant differences among the four spawns in embryo survival 1 day after egg collection, hatching percentage and larval survival 4 days after egg collection, but there was no consistent trend during the study. Significant positive correlations existed between 4-day larval survival and hatching percentage (P=0.0002), and between hatching percentage and embryo survival 1 day after egg collection (P=0.03), indicating that 1-day embryo survival can be a potential biomarker for egg quality in commercial hatcheries. The results of the study demonstrate that European sea bass can produce up to four sequential spawns of high quality eggs in response to repeated acute treatments of GnRHa, and suggest that a pulsatile treatment of GnRHa may be necessary to induce the appropriate cycles of hormone changes for the recruitment, maturation and ovulation of multiple batches of viable eggs. © 2003 Elsevier Science B.V. All rights reserved. |
Mandalakis, M; Gustafsson, Ö Journal of Chromatography A, 996 (1-2), pp. 163–172, 2003, ISSN: 00219673, (Publisher: Elsevier). @article{mandalakis_optimization_2003, title = {Optimization of a preparative capillary gas chromatography-mass spectrometry system for the isolation and harvesting of individual polycyclic aromatic hydrocarbons}, author = {M Mandalakis and Ö Gustafsson}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0038021269&doi=10.1016%2fS0021-9673%2803%2900612-5&partnerID=40&md5=e062f00b78a8f49e937856db9a1d8a87}, doi = {10.1016/S0021-9673(03)00612-5}, issn = {00219673}, year = {2003}, date = {2003-01-01}, journal = {Journal of Chromatography A}, volume = {996}, number = {1-2}, pages = {163--172}, abstract = {Operation parameters of a preparative capillary gas chromatography (pcGC) system were optimized to facilitate clean and efficient harvesting of individual polycyclic aromatic hydrocarbons (PAHs) for subsequent compound-specific radiocarbon analysis. For PAHs, the recommended optimized settings of the specially-designed pcGC cooled injection system (CIS) and preparative fraction collector (PFC) are: 5 s CIS solvent venting time, deactivation of CIS "stop flow" injection mode, autoinjector "fast injection" mode, 60 s CIS splitless time, 340°C PFC switch temperature, and 30°C (ambient) trapping temperature. These optimized conditions yielded highly reproducible, pure, and efficient pcGC harvesting of six PAHs with mass recoveries of 90-100% and purity of the isolates of 97-100%. © 2003 Elsevier Science B.V. All rights reserved.}, note = {Publisher: Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {article} } Operation parameters of a preparative capillary gas chromatography (pcGC) system were optimized to facilitate clean and efficient harvesting of individual polycyclic aromatic hydrocarbons (PAHs) for subsequent compound-specific radiocarbon analysis. For PAHs, the recommended optimized settings of the specially-designed pcGC cooled injection system (CIS) and preparative fraction collector (PFC) are: 5 s CIS solvent venting time, deactivation of CIS "stop flow" injection mode, autoinjector "fast injection" mode, 60 s CIS splitless time, 340°C PFC switch temperature, and 30°C (ambient) trapping temperature. These optimized conditions yielded highly reproducible, pure, and efficient pcGC harvesting of six PAHs with mass recoveries of 90-100% and purity of the isolates of 97-100%. © 2003 Elsevier Science B.V. All rights reserved. |
Rigos, G; Nengas, I; Tyrpenou, A E; Alexis, M; Troisi, G M Pharmacokinetics and bioavailability of oxytetracycline in gilthead sea bream (Sparus aurata) after a single dose Journal Article Aquaculture, 221 , pp. 75 – 83, 2003. @article{rigos_pharmacokinetics_2003, title = {Pharmacokinetics and bioavailability of oxytetracycline in gilthead sea bream (Sparus aurata) after a single dose}, author = {G Rigos and I Nengas and A E Tyrpenou and M Alexis and G M Troisi}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0037402546&partnerID=40&md5=73df0faf1da29455a07de80aa4cde170}, year = {2003}, date = {2003-01-01}, journal = {Aquaculture}, volume = {221}, pages = {75 -- 83}, abstract = {This is the first study to investigate the pharmacokinetic parameters of oxytetracycline (OTC) in gilthead sea bream (Sparus aurata). Following a single intravascular injection of OTC (40 mg/kg) in 100 g fish kept at 20°C, the distribution half-life (t1/2α) and the elimination half-life (t1/2β) of OTC were found to be long (2 and 53 h, respectively). The drug was well distributed throughout the internal body compartments since the apparent volume of distribution of the drug at steady-state (Vd(ss)) and the apparent volume of distribution by the area method (Vd(arca)) were found to be high (2.9 and 3.8 l/kg, respectively). OTC had a long mean residence time (MRT) (56 h) but a short total clearance (CLT) (0.05 1/kg h). The slow elimination of the drug suggests a need for long withdrawal periods prior to use of dosed fish for human consumption. Although the bioavailability (F%) of OTC following oral administration in aqueous suspension (75 mg/kg) was calculated to be low (9%), maximum OTC plasma concentration was relatively high (2.5 μg/ml), indicating that current therapeutic regimens applied to gilthead sea bream might be effective against bacterial pathogens. © 2003 Elsevier Science B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This is the first study to investigate the pharmacokinetic parameters of oxytetracycline (OTC) in gilthead sea bream (Sparus aurata). Following a single intravascular injection of OTC (40 mg/kg) in 100 g fish kept at 20°C, the distribution half-life (t1/2α) and the elimination half-life (t1/2β) of OTC were found to be long (2 and 53 h, respectively). The drug was well distributed throughout the internal body compartments since the apparent volume of distribution of the drug at steady-state (Vd(ss)) and the apparent volume of distribution by the area method (Vd(arca)) were found to be high (2.9 and 3.8 l/kg, respectively). OTC had a long mean residence time (MRT) (56 h) but a short total clearance (CLT) (0.05 1/kg h). The slow elimination of the drug suggests a need for long withdrawal periods prior to use of dosed fish for human consumption. Although the bioavailability (F%) of OTC following oral administration in aqueous suspension (75 mg/kg) was calculated to be low (9%), maximum OTC plasma concentration was relatively high (2.5 μg/ml), indicating that current therapeutic regimens applied to gilthead sea bream might be effective against bacterial pathogens. © 2003 Elsevier Science B.V. All rights reserved. |
Rigos, G; Tyrpenou, A E; Nengas, I; Yiagnisis, M; Koutsodimou, M; Alexis, M; Troisi, G M Pharmacokinetics of flumequine and in vitro activity against bacterial pathogens of gilthead sea bream Sparus aurata Journal Article Diseases of Aquatic Organisms, 54 , pp. 35 – 41, 2003. @article{rigos_pharmacokinetics_2003-1, title = {Pharmacokinetics of flumequine and in vitro activity against bacterial pathogens of gilthead sea bream Sparus aurata}, author = {G Rigos and A E Tyrpenou and I Nengas and M Yiagnisis and M Koutsodimou and M Alexis and G M Troisi}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0242500738&partnerID=40&md5=04952ed6946b3b5bf6b53e7fa6783038}, year = {2003}, date = {2003-01-01}, journal = {Diseases of Aquatic Organisms}, volume = {54}, pages = {35 -- 41}, abstract = {The present study investigated the kinetic profile of flumequine (FLU) in gilthead sea bream Sparus aurata (170 g) held at 19°C and evaluated its in vitro efficacy against important bacterial diseases in Mediterranean mariculture. Following a single intravascular injection (10 mg kg-1 fish), the distribution half-life (t1/2α) and the half-life of the terminal phase of elimination (t1/2α) of the drug were 0.2 and 30 h respectively. Tissue penetration of FLU was low, since both the apparent distribution volume of the drug at steady-state (Vd(ss)) and the apparent volume of the central compartment (Vc) were small (0.57 and 0.15 1 kg-1). The mean residence time (MRT) was short (11 h) and the total clearance (CLT) of the drug was slow (0.05 1 kg-1 h-1). Following oral administration (20 mg kg1), the bioavailability (F%) of FLU was 29% and the maximum plasma concentration was 1.7 pg ml-1. The minimum inhibitory concentration (MIC) of the drug in distilled water supplemented with 2% NaCl against Vibrio anguillarum Serotype 1b, Photobacterium damsela ssp. piscicida, V. alginolyticus, V. damsela and V. fluvialis was 0.15, 0.3, 1.2, 0.019 and 0.15 pg ml-1 respectively. The addition however of 10 mM Ca2+ and 55 mM Mg2+ to the medium resulted in an 8- to >120-fold reduction in FLU activity. The results indicate that FLU has an adequate kinetic profile in gilthead sea bream and that marine cations induce a significant impact on the activity of FLU, rendering its use against bacterial pathogens questionable.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The present study investigated the kinetic profile of flumequine (FLU) in gilthead sea bream Sparus aurata (170 g) held at 19°C and evaluated its in vitro efficacy against important bacterial diseases in Mediterranean mariculture. Following a single intravascular injection (10 mg kg-1 fish), the distribution half-life (t1/2α) and the half-life of the terminal phase of elimination (t1/2α) of the drug were 0.2 and 30 h respectively. Tissue penetration of FLU was low, since both the apparent distribution volume of the drug at steady-state (Vd(ss)) and the apparent volume of the central compartment (Vc) were small (0.57 and 0.15 1 kg-1). The mean residence time (MRT) was short (11 h) and the total clearance (CLT) of the drug was slow (0.05 1 kg-1 h-1). Following oral administration (20 mg kg1), the bioavailability (F%) of FLU was 29% and the maximum plasma concentration was 1.7 pg ml-1. The minimum inhibitory concentration (MIC) of the drug in distilled water supplemented with 2% NaCl against Vibrio anguillarum Serotype 1b, Photobacterium damsela ssp. piscicida, V. alginolyticus, V. damsela and V. fluvialis was 0.15, 0.3, 1.2, 0.019 and 0.15 pg ml-1 respectively. The addition however of 10 mM Ca2+ and 55 mM Mg2+ to the medium resulted in an 8- to >120-fold reduction in FLU activity. The results indicate that FLU has an adequate kinetic profile in gilthead sea bream and that marine cations induce a significant impact on the activity of FLU, rendering its use against bacterial pathogens questionable. |
Berrebi, P; Tsigenopoulos, C S Phylogenetic organization of the genus Barbus sensu stricto: A review based on data using molecular markers Incollection Banarescu, P; Bogutskaya, N G (Ed.): The Freshwater Fishes of Europe, Vol. 5/II: Cyprinidae, Part II: Barbus, pp. 11–22, AULA-Verlag, 2003, (Backup Publisher: AULA-Verlag). @incollection{berrebi_phylogenetic_2003, title = {Phylogenetic organization of the genus Barbus sensu stricto: A review based on data using molecular markers}, author = {P Berrebi and C S Tsigenopoulos}, editor = {P Banarescu and N G Bogutskaya}, year = {2003}, date = {2003-01-01}, booktitle = {The Freshwater Fishes of Europe, Vol. 5/II: Cyprinidae, Part II: Barbus}, pages = {11--22}, publisher = {AULA-Verlag}, abstract = {In this review, we are providing all available information on the molecular phylogeny of the genus textitBarbus sensu lato. The number of chromosomes indicates whether each species originated as diploid (50), tetraploid (100), or hexaploid (150) fishes. This is considered the primary parameter of classification. Only the "true barbs," which are the European, West Asian, and North African tetraploids, are discussed in the text species by species. Special care is taken to provide an accurate phylogeny of the European species. The main conclusion reached through the molecular studies is that it is necessary to limit the genus textitBarbus to the "true barbs" and to give different generic names to all other taxa originally included in textitBarbus sensu lato}, note = {Backup Publisher: AULA-Verlag}, keywords = {}, pubstate = {published}, tppubtype = {incollection} } In this review, we are providing all available information on the molecular phylogeny of the genus textitBarbus sensu lato. The number of chromosomes indicates whether each species originated as diploid (50), tetraploid (100), or hexaploid (150) fishes. This is considered the primary parameter of classification. Only the "true barbs," which are the European, West Asian, and North African tetraploids, are discussed in the text species by species. Special care is taken to provide an accurate phylogeny of the European species. The main conclusion reached through the molecular studies is that it is necessary to limit the genus textitBarbus to the "true barbs" and to give different generic names to all other taxa originally included in textitBarbus sensu lato |
Tsigenopoulos, C S; Durand, J D; Ünlü, E; Berrebi, P Biological Journal of the Linnean Society, 80 , pp. 207 – 222, 2003. @article{tsigenopoulos_rapid_2003, title = {Rapid radiation of the Mediterranean Luciobarbus species (Cyprinidae) after the Messinian salinity crisis of the Mediterranean Sea, inferred from mitochondrial phylogenetic analysis}, author = {C S Tsigenopoulos and J D Durand and E Ünlü and P Berrebi}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0142095131&partnerID=40&md5=b366bb24a4425150565d56b1f334c4ee}, year = {2003}, date = {2003-01-01}, journal = {Biological Journal of the Linnean Society}, volume = {80}, pages = {207 -- 222}, abstract = {Phylogenetic relationships of 64 freshwater Barbus s.s. species distributed in basins around the Mediterranean Sea were assessed using cytochrome b sequences. Our results are in concordance with previous morphological and genetic studies, which proposed that these species belong to two major lineages (or subgenera): Barbus and Luciobarbus. We were particularly interested in phylogenetic relationships among species of the Luciobarbus lineage that are primarily found in the southern Mediterranean region from the Iberian Peninsula to the Middle East. In the Luciobarbus lineage, species that were previously attributed to the Capoeta genus were clustered. In this study, we observed short internodes between monophyletic groups having a geographical agreement around the Mediterranean. However, groups from the opposite sides of the Mediterranean Sea (Iberian Peninsula-Capoeta, north-western Africa-Middle East) seem to be phylogenetically close. We therefore infer that rapid radiation of Luciobarbus species in the Late Miocene better fits our data rather than gradual founder events in the southern Mediterranean. We propose that the biogeographical event along an east-west route, responsible for the present distribution of Luciobarbus species, was the ’Lago Mare’ phase of the Mediterranean Sea that provided a rapid dispersal route over extensive distances. This provides new insights into the speciation pattern of this group, and may be of general use in the study of freshwater species in these regions. © 2003 The Linnean Society of London.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Phylogenetic relationships of 64 freshwater Barbus s.s. species distributed in basins around the Mediterranean Sea were assessed using cytochrome b sequences. Our results are in concordance with previous morphological and genetic studies, which proposed that these species belong to two major lineages (or subgenera): Barbus and Luciobarbus. We were particularly interested in phylogenetic relationships among species of the Luciobarbus lineage that are primarily found in the southern Mediterranean region from the Iberian Peninsula to the Middle East. In the Luciobarbus lineage, species that were previously attributed to the Capoeta genus were clustered. In this study, we observed short internodes between monophyletic groups having a geographical agreement around the Mediterranean. However, groups from the opposite sides of the Mediterranean Sea (Iberian Peninsula-Capoeta, north-western Africa-Middle East) seem to be phylogenetically close. We therefore infer that rapid radiation of Luciobarbus species in the Late Miocene better fits our data rather than gradual founder events in the southern Mediterranean. We propose that the biogeographical event along an east-west route, responsible for the present distribution of Luciobarbus species, was the ’Lago Mare’ phase of the Mediterranean Sea that provided a rapid dispersal route over extensive distances. This provides new insights into the speciation pattern of this group, and may be of general use in the study of freshwater species in these regions. © 2003 The Linnean Society of London. |
Mylonas, C C; Papadaki, M; Divanach, P Seasonal changes in sperm production and quality in the red porgy Pagrus pagrus (L.) Journal Article Aquaculture Research, 34 (13), pp. 1161–1170, 2003, ISSN: 1355557X. @article{mylonas_seasonal_2003, title = {Seasonal changes in sperm production and quality in the red porgy Pagrus pagrus (L.)}, author = {C C Mylonas and M Papadaki and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0242286610&doi=10.1046%2fj.1365-2109.2003.00922.x&partnerID=40&md5=035f37a6d19b84e81a7004fb96f03fec}, doi = {10.1046/j.1365-2109.2003.00922.x}, issn = {1355557X}, year = {2003}, date = {2003-01-01}, journal = {Aquaculture Research}, volume = {34}, number = {13}, pages = {1161--1170}, abstract = {Cultured red porgy Pagrus pagrus (L.) males (n = 6) were sampled every 2 weeks for milt, in order to monitor changes in sperm quality parameters during a whole spawning period. On 11 January 2001, 60% of the fish were spermiating, increasing to 100% in mid-February and dropping to 30% by mid-April. Sperm density showed a slight increasing trend, with mean values ranging between 8.6 and 23.7 × 109 spermatozoa mL-1. Sperm motility percentage exhibited a significant improvement during the spawning season (analysis of variance (ANOVA) P = 0.0001). The duration of forward motility for the major part of the monitoring period ranged between 2 and 4 min. Red porgy spermatozoa maintained their viability for many days after whole storage of milt at 4°C. During the monitoring period there were significant changes in the mean duration of sperm survival after cold storage, ranging from 5 to 12 days. The total volume of expressible milt was maximal on 28 March, increasing from a mean value of 1.7 mL to 5.3 mL kg-1. Milt production of captive-reared red porgy does not appear to be limiting, when compared with the volume of expressible milt produced by other cultured marine fishes.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Cultured red porgy Pagrus pagrus (L.) males (n = 6) were sampled every 2 weeks for milt, in order to monitor changes in sperm quality parameters during a whole spawning period. On 11 January 2001, 60% of the fish were spermiating, increasing to 100% in mid-February and dropping to 30% by mid-April. Sperm density showed a slight increasing trend, with mean values ranging between 8.6 and 23.7 × 109 spermatozoa mL-1. Sperm motility percentage exhibited a significant improvement during the spawning season (analysis of variance (ANOVA) P = 0.0001). The duration of forward motility for the major part of the monitoring period ranged between 2 and 4 min. Red porgy spermatozoa maintained their viability for many days after whole storage of milt at 4°C. During the monitoring period there were significant changes in the mean duration of sperm survival after cold storage, ranging from 5 to 12 days. The total volume of expressible milt was maximal on 28 March, increasing from a mean value of 1.7 mL to 5.3 mL kg-1. Milt production of captive-reared red porgy does not appear to be limiting, when compared with the volume of expressible milt produced by other cultured marine fishes. |
Machias, A; Somarakis, S; Papandroulakis, N; Spedicato, M -T; Suquet, M; Lembo, G; Divanach, P Settlement of the wreckfish (Polyprion americanus) Journal Article Marine Biology, 142 (1), pp. 45–52, 2003, ISSN: 0025-3162. @article{machias_settlement_2003, title = {Settlement of the wreckfish (Polyprion americanus)}, author = {A Machias and S Somarakis and N Papandroulakis and M -T Spedicato and M Suquet and G Lembo and P Divanach}, url = {http://link.springer.com/10.1007/s00227-002-0918-2}, doi = {10.1007/s00227-002-0918-2}, issn = {0025-3162}, year = {2003}, date = {2003-01-01}, urldate = {2020-08-18}, journal = {Marine Biology}, volume = {142}, number = {1}, pages = {45--52}, abstract = {The wreckfish (Polyprion americanus) is a panoceanic, long-lived, deep-water demersal species, characterized by an extended pelagic juvenile phase associated with floating objects. In the present study morphometric data from 435 specimens collected from Greece (Crete), Italy (Ionian Sea) and the Atlantic coast of France from September 1999 to March 2001 were analyzed to estimate the settlement size of the species and associated changes in morphology. The fishery sample included specimens from both the pelagic and the demersal stage. Length-at-settlement (TL50) and 95% confidence intervals were estimated by fitting a logistic function. Eleven morphometric characters were analyzed, and the existing inflection points, reflecting alterations in body shape, were defined: (1) iteratively, as the transition point, splitting the data set into two groups, for which the reduced major axis functions, between a character and total length, of the successive groups best fit (minimum sum of squares of residuals) the combined data set and (2) where the second derivative of the fitted third-order polynomial functions to morphometric ratios equaled zero. The main size range within which wreckfish settled was 56-65 cm, and the means of the inflection points defined by the two methods were 61 and 64 cm. These results indicate changes in functional morphology associated with settlement. The monitoring of wreckfish juveniles caught in the wild and kept individually in captivity showed changes in growth and food intake at approximately the estimated settlement length and suggested that temperature was the most likely factor triggering settlement.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The wreckfish (Polyprion americanus) is a panoceanic, long-lived, deep-water demersal species, characterized by an extended pelagic juvenile phase associated with floating objects. In the present study morphometric data from 435 specimens collected from Greece (Crete), Italy (Ionian Sea) and the Atlantic coast of France from September 1999 to March 2001 were analyzed to estimate the settlement size of the species and associated changes in morphology. The fishery sample included specimens from both the pelagic and the demersal stage. Length-at-settlement (TL50) and 95% confidence intervals were estimated by fitting a logistic function. Eleven morphometric characters were analyzed, and the existing inflection points, reflecting alterations in body shape, were defined: (1) iteratively, as the transition point, splitting the data set into two groups, for which the reduced major axis functions, between a character and total length, of the successive groups best fit (minimum sum of squares of residuals) the combined data set and (2) where the second derivative of the fitted third-order polynomial functions to morphometric ratios equaled zero. The main size range within which wreckfish settled was 56-65 cm, and the means of the inflection points defined by the two methods were 61 and 64 cm. These results indicate changes in functional morphology associated with settlement. The monitoring of wreckfish juveniles caught in the wild and kept individually in captivity showed changes in growth and food intake at approximately the estimated settlement length and suggested that temperature was the most likely factor triggering settlement. |
Prinos, P; Koutandos, E; Karambas, T Spectral evolution of breaking-non breaking waves in the surf zone Inproceedings pp. 312–313, 2003, (Publication Title: 29th International Conference on Coastal Engineering). @inproceedings{prinos_spectral_2003, title = {Spectral evolution of breaking-non breaking waves in the surf zone}, author = {P Prinos and E Koutandos and T Karambas}, year = {2003}, date = {2003-01-01}, volume = {1}, pages = {312--313}, note = {Publication Title: 29th International Conference on Coastal Engineering}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Publications
2004 |
Feeding rates, growth performance and gametogenesis of wreckfish (Polyprion americanus) kept in captivity Journal Article Aquaculture International, 12 , pp. 395 – 407, 2004. |
Induction of spawning of cultured greater amberjack (Seriola dumerili) using GnRHa implants Journal Article Aquaculture, 237 (1-4), pp. 141–154, 2004, ISSN: 00448486. |
Genome, 47 , pp. 1154–1163, 2004. |
Macronutrient selection by sharp snout seabream (Diplodus puntazzo) Journal Article Aquaculture, 232 (1-4), pp. 481–491, 2004, ISSN: 00448486. |
Mesocosm: A reliable technology for larval rearing of Diplodus puntazzo and Diplodus sargus sargus Journal Article Aquaculture International, 12 , pp. 345 – 355, 2004. |
Mitochondrial DNA Incollection Cadrin, S; Friedland, K; Waldman, J (Ed.): Stock Ιdentification Μethods, pp. 311–330, ELSEVIER Academic Press, 2004, (Backup Publisher: ELSEVIER Academic Press Section: 16). |
Pharmacokinetics of cephalexin in sea bream, Sparus aurata (L.), after a single intraperitoneal injection Journal Article Journal of Applied Ichthyology, 20 (5), pp. 422–426, 2004, ISSN: 01758659. |
Poor bioavailability of oxytetracycline in sharpsnout sea bream Diplodus puntazzo Journal Article Aquaculture, 235 , pp. 489 – 497, 2004. |
Potential drug (oxytetracycline and oxolinic acid) pollution from Mediterranean sparid fish farms Journal Article Aquatic Toxicology, 69 , pp. 281 – 288, 2004. |
Radiocarbon apportionment of fossil versus biofuel combustion sources of polycyclic aromatic hydrocarbons in the Stockholm metropolitan area Journal Article Environmental Science and Technology, 38 (20), pp. 5344–5349, 2004, ISSN: 0013936X. |
Reproductive biology of the shi drum (Umrina cirrosa) in captivity and induction of spawning using GNRHA Journal Article Israeli Journal of Aquaculture - Bamidgeh, 56 (2), pp. 77–94, 2004, ISSN: 0792156X. |
The kinetic profile of oxolinic acid in sharpsnout sea bream, Diplodus puntazzo (Cetti 1777) Journal Article Aquaculture Research, 35 , pp. 1299 – 1304, 2004. |
Wet deposition of polychlorinated biphenyls in the eastern Mediterranean Journal Article Environmental Science and Technology, 38 (11), pp. 3011–3018, 2004, ISSN: 0013936X. |
2003 |
Discord in the family Sparidae (Teleostei): divergent phylogeographical patterns across the Atlantic-Mediterranean divide Journal Article Journal of Evolutionary Biology, 16 (6), pp. 1149–1158, 2003, ISSN: 1010-061X, 1420-9101. |
The genetic architecture of correlations among growth-related traits and male age at maturation in rainbow trout Journal Article Journal of Fish Biology, 63 (3), pp. 746–764, 2003, ISSN: 0022-1112, 1095-8649. |
Computer assisted evaluation of wound healing in chronic ulcers Journal Article Computers in Biology and Medicine, 33 (4), pp. 311–317, 2003, ISSN: 00104825. |
Aquaculture, 225 (1-4), pp. 207–223, 2003, ISSN: 00448486. |
Aquaculture, 225 (1-4), pp. 309–323, 2003, ISSN: 00448486. |
Aquaculture, 225 (1-4), pp. 109–119, 2003, ISSN: 00448486. |
Inducible transgenic mice reveal resting dendritic cells as potent inducers of CD8(+) Ŧ cell tolerance Journal Article Immunity, 18 (5), pp. 713–720, 2003, (ISBN: 1074-7613). |
Seasonal patterns of spoilage of ice-stored cultured gilthead sea bream (Sparus aurata) Journal Article Food Chemistry, 81 (2), pp. 263–268, 2003, ISSN: 03088146. |
Aquaculture, 220 (1-4), pp. 633–642, 2003, ISSN: 00448486. |
Development of a saddleback-like syndrome in reared white seabream Diplodus sargus (Linnaeus, 1758) Journal Article Aquaculture, 217 (1-4), pp. 673–676, 2003, ISSN: 00448486. |
A new apparatus for the direct measurement of otter trawling effects on the epibenthic and hyperbenthic macrofauna Journal Article Journal of the Marine Biological Association of the United Kingdom, 83 (6), pp. 1363–1368, 2003, ISSN: 00253154. |
A numerical and experimental study on the hydrodynamics of a heave motion floating breakwater Inproceedings 2003, (Publication Title: XXX IAHR Congress). |
Amphipod community structure in an area of intensive natural and antropogenic disturbance (Termaicos Bay in the Mediteranean Sea). Inproceedings Tunis, Tunisia, 2003, (Publication Title: The11th International Colloquium on Amphipoda, Type: Abstract). |
An application of different bioindicators for assessing water quality: A case study in the rivers Alfeios and Pineios (Peloponnisos, Greece) Journal Article Ecological Indicators, 2 (4), pp. 345–360, 2003, ISSN: 1470160X, (Publisher: Elsevier). |
Boussinesq modeling of the alongshore sediment transport Inproceedings 2003, (Publication Title: XXX IAHR Congress). |
Direct evidence for destruction of polychlorobiphenyls by OH radicals in the subtropical troposphere Journal Article Environmental Science and Technology, 37 (3), pp. 542–547, 2003, ISSN: 0013936X. |
DNA microsatellite markers in service of swordfish stock-structure analysis in the Atlantic and Mediterranean Journal Article ICCAT Col. Vol. Sci. Papers, 55 (4), pp. 1632–1639, 2003. |
Effect of incubation experiments on the distribution of different lipid classes (aliphatic hydrocarbons, diglycerides, free fatty acids, glycolipids, phospholipids) in sediments of the Cretan Sea Inproceedings Hersonissos, Crete, Greece, 2003, (Publication Title: 7th Symposium of Oceanography & Fisheries Type: Poster). |
Eleven new microsatellites of the sea bass (Dicentrarchus labrax L.) Journal Article Molecular Ecology Notes, 3 (3), pp. 352–354, 2003, ISSN: 14718278. |
Enhancement of spermiation in European sea bass (Dicentrarchus labrax) at the end of the reproductive season using GnRHa implants Journal Article Aquaculture, 219 (1-4), pp. 873–890, 2003, ISSN: 00448486, (Publisher: Elsevier). |
Evaluation of transportation procedures on water quality and fry performance in red porgy (Pagrus pagrus) fry Journal Article Aquaculture, 218 , pp. 187 – 202, 2003. |
Floating breakwaters interaction with waves-A large scale experimental study Inproceedings pp. 385–392, 2003, (Publication Title: XXX IAHR Congress). |
Gene expression of estrogen receptors α and β during early sexual differentiation in the European sea bass (Dicentrarchus labrax) Journal Article Fish Physiology and Biochemistry, 28 (1-4), pp. 175, 2003, ISSN: 09201742. |
Chimia, 57 (9), pp. 505–508, 2003, ISSN: 00094293, (Publisher: Swiss Chemical Society). |
Induction of ovulation in captive-reared dusky grouper, Epinephelus marginatus (Lowe, 1834), with a sustained-release GnRHa implant Journal Article Aquaculture, 219 (1-4), pp. 841–858, 2003, ISSN: 00448486, (Publisher: Elsevier). |
Influence of rearing temperature at two periods during early life on growth and sex differentiation of two strains of European sea bass Journal Article Fish Physiology and Biochemistry, 28 (1-4), pp. 167–168, 2003, ISSN: 09201742. |
Loss Processes of PCBs in the subtropical atmosphere: Study of PCB-OH radical reactions in eastern Mediterranean Inproceedings Organohalogen Compounds, pp. 2, 2003. |
Molecular phylogeny and biogeography of the wall-lizard Podarcis erhardii (Squamata: Lacertidae) Journal Article Molecular Phylogenetics and Evolution, 28 , pp. 38–46, 2003. |
Multiple spawning and egg quality of individual European sea bass (Dicentrarchus labrax) females after repeated injections of GnRHa Journal Article Aquaculture, 221 (1-4), pp. 605–620, 2003, ISSN: 00448486, (Publisher: Elsevier). |
Journal of Chromatography A, 996 (1-2), pp. 163–172, 2003, ISSN: 00219673, (Publisher: Elsevier). |
Pharmacokinetics and bioavailability of oxytetracycline in gilthead sea bream (Sparus aurata) after a single dose Journal Article Aquaculture, 221 , pp. 75 – 83, 2003. |
Pharmacokinetics of flumequine and in vitro activity against bacterial pathogens of gilthead sea bream Sparus aurata Journal Article Diseases of Aquatic Organisms, 54 , pp. 35 – 41, 2003. |
Phylogenetic organization of the genus Barbus sensu stricto: A review based on data using molecular markers Incollection Banarescu, P; Bogutskaya, N G (Ed.): The Freshwater Fishes of Europe, Vol. 5/II: Cyprinidae, Part II: Barbus, pp. 11–22, AULA-Verlag, 2003, (Backup Publisher: AULA-Verlag). |
Biological Journal of the Linnean Society, 80 , pp. 207 – 222, 2003. |
Seasonal changes in sperm production and quality in the red porgy Pagrus pagrus (L.) Journal Article Aquaculture Research, 34 (13), pp. 1161–1170, 2003, ISSN: 1355557X. |
Settlement of the wreckfish (Polyprion americanus) Journal Article Marine Biology, 142 (1), pp. 45–52, 2003, ISSN: 0025-3162. |
Spectral evolution of breaking-non breaking waves in the surf zone Inproceedings pp. 312–313, 2003, (Publication Title: 29th International Conference on Coastal Engineering). |