2004 |
Koutandos, E V; Karambas, Th. V; Koutitas, C G Floating Breakwater Response to Waves Action Using a Boussinesq Model Coupled with a 2DV Elliptic Solver Journal Article Journal of Waterway, Port, Coastal, and Ocean Engineering, 130 (5), pp. 243–255, 2004, ISSN: 0733-950X, 1943-5460. @article{koutandos_floating_2004, title = {Floating Breakwater Response to Waves Action Using a Boussinesq Model Coupled with a 2DV Elliptic Solver}, author = {E V Koutandos and Th. V Karambas and C G Koutitas}, url = {http://ascelibrary.org/doi/10.1061/%28ASCE%290733-950X%282004%29130%3A5%28243%29}, doi = {10.1061/(ASCE)0733-950X(2004)130:5(243)}, issn = {0733-950X, 1943-5460}, year = {2004}, date = {2004-09-01}, urldate = {2020-08-18}, journal = {Journal of Waterway, Port, Coastal, and Ocean Engineering}, volume = {130}, number = {5}, pages = {243--255}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Kasapidis, P; Planes, S; Laurent, V; Quinta, R; Teia, A; Lenfant, R; Darivianakis, S; Terzoglou, V; Kotoulas, G; Magoulas, A Stock discrimination and temporal and spatial genetic variation of sardine (Sardina pilchardus) in northeastern Atlantic, with a combined analysis of nuclear (microsatellites and allozymes) and mitochondrial DNA markers. Inproceedings Kasapidis, P (Ed.): 2004 ICES Annual Science Conference, 2004. @inproceedings{kasapidis_stock_2004, title = {Stock discrimination and temporal and spatial genetic variation of sardine (Sardina pilchardus) in northeastern Atlantic, with a combined analysis of nuclear (microsatellites and allozymes) and mitochondrial DNA markers.}, author = {P Kasapidis and S Planes and V Laurent and R Quinta and A Teia and R Lenfant and S Darivianakis and V Terzoglou and G Kotoulas and A Magoulas}, editor = {P Kasapidis}, year = {2004}, date = {2004-09-01}, booktitle = {2004 ICES Annual Science Conference}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Sfakianakis, D G; Koumoundouros, G; Divanach, P; Kentouri, M Aquaculture, 232 (1-4), pp. 407–424, 2004, ISSN: 00448486. @article{sfakianakis_osteological_2004, title = {Osteological development of the vertebral column and of the fins in Pagellus erythrinus (L. 1758). Temperature effect on the developmental plasticity and morpho-anatomical abnormalities}, author = {D G Sfakianakis and G Koumoundouros and P Divanach and M Kentouri}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848603005623}, doi = {10.1016/j.aquaculture.2003.08.014}, issn = {00448486}, year = {2004}, date = {2004-04-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {232}, number = {1-4}, pages = {407--424}, abstract = {The osteological ontogeny of Pagellus erythrinus (Linnaeus, 1758) and its temperature-induced plasticity is studied. The ontogeny of the skeleton follows the pattern described in all other Sparidae studied so far: the onset of the ontogeny of the vertebral column and the caudal fin is at 4.3 mm total length (TL) and the completion at 17.6 and 15.4 mm TL, respectively. Dorsal and anal fin development appears at about 5.2 mm TL and completes at 7.5 mm TL. The pelvic are the last fins to develop (7.5-11.5 mm TL). Although the pectorals are the only fins present before first feeding, their ontogeny is completed only after 12.0 mm TL. Temperature affects the developmental rate of the skeleton giving as much as 10.9% of differentiation in total length between 16 and 21°C (caudal lepidotrichia), 9.2% between 16 and 18°C (caudal lepidotrichia) and 6.7% between 18 and 21°C (pelvic lepidotrichia). The role of temperature in developmental plasticity is enhanced by the induction of abnormalities mainly in the area of the caudal fin, which reached (in total) 75% occurrence in the higher temperature regime (23°C) tested. The results of the skeletal ontogeny are compared with the pattern of development of the Sparidae, and are discussed in respect to the role of temperature in the developmental plasticity of fish. © 2004 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The osteological ontogeny of Pagellus erythrinus (Linnaeus, 1758) and its temperature-induced plasticity is studied. The ontogeny of the skeleton follows the pattern described in all other Sparidae studied so far: the onset of the ontogeny of the vertebral column and the caudal fin is at 4.3 mm total length (TL) and the completion at 17.6 and 15.4 mm TL, respectively. Dorsal and anal fin development appears at about 5.2 mm TL and completes at 7.5 mm TL. The pelvic are the last fins to develop (7.5-11.5 mm TL). Although the pectorals are the only fins present before first feeding, their ontogeny is completed only after 12.0 mm TL. Temperature affects the developmental rate of the skeleton giving as much as 10.9% of differentiation in total length between 16 and 21°C (caudal lepidotrichia), 9.2% between 16 and 18°C (caudal lepidotrichia) and 6.7% between 18 and 21°C (pelvic lepidotrichia). The role of temperature in developmental plasticity is enhanced by the induction of abnormalities mainly in the area of the caudal fin, which reached (in total) 75% occurrence in the higher temperature regime (23°C) tested. The results of the skeletal ontogeny are compared with the pattern of development of the Sparidae, and are discussed in respect to the role of temperature in the developmental plasticity of fish. © 2004 Elsevier B.V. All rights reserved. |
Alarcón, J A; Magoulas, A; Georgakopoulos, T; Zouros, E; Alvarez, M C Genetic comparison of wild and cultivated European populations of the gilthead sea bream (Sparus aurata) Journal Article Aquaculture, 230 (1-4), pp. 65–80, 2004, ISSN: 00448486. @article{alarcon_genetic_2004, title = {Genetic comparison of wild and cultivated European populations of the gilthead sea bream (Sparus aurata)}, author = {J A Alarcón and A Magoulas and T Georgakopoulos and E Zouros and M C Alvarez}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848603004344}, doi = {10.1016/S0044-8486(03)00434-4}, issn = {00448486}, year = {2004}, date = {2004-02-01}, urldate = {2020-08-09}, journal = {Aquaculture}, volume = {230}, number = {1-4}, pages = {65--80}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Katharios, P; Pavlidis, M; Iliopoulou-Georgudaki, J Accumulation of ivermectin in the brain of sea bream, Sparus aurata after intraperitoneal administration Journal Article Environmental Toxicology and Pharmacology, 17 (1), pp. 9–12, 2004, ISSN: 13826689. @article{katharios_accumulation_2004, title = {Accumulation of ivermectin in the brain of sea bream, Sparus aurata after intraperitoneal administration}, author = {P Katharios and M Pavlidis and J Iliopoulou-Georgudaki}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-2342451297&doi=10.1016%2fj.etap.2004.01.003&partnerID=40&md5=a461dd2f65ec8b5bfa8e6a22e6b2677d}, doi = {10.1016/j.etap.2004.01.003}, issn = {13826689}, year = {2004}, date = {2004-01-01}, journal = {Environmental Toxicology and Pharmacology}, volume = {17}, number = {1}, pages = {9--12}, abstract = {Ivermectin, which is widely used in veterinary and human, has been considered safe due to its inability to penetrate into the central nervous system of higher vertebrates. This paper presents data on the ability of the drug to cross the blood-brain barrier of the marine teleost sea bream, Sparus aurata and accumulate in the brain. The concentration of the drug in the brain and the serum of the fish was assessed by the use of a direct competitive ELISA commercial kit. Our results showed a rapid uptake of the substance by the brain of the fish reaching a maximum concentration of 98.9 ng g-1 8 h post treatment. The trend of the absorption of the drug in brain followed that of the blood. Concentration of the drug in the brain remained high at each sampling point over the 24 h duration of the experiment. In view of these findings, the need of study of the role of the blood-brain barrier and particularly the multidrug resistance mechanism in sea bream is outlined. © 2004 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Ivermectin, which is widely used in veterinary and human, has been considered safe due to its inability to penetrate into the central nervous system of higher vertebrates. This paper presents data on the ability of the drug to cross the blood-brain barrier of the marine teleost sea bream, Sparus aurata and accumulate in the brain. The concentration of the drug in the brain and the serum of the fish was assessed by the use of a direct competitive ELISA commercial kit. Our results showed a rapid uptake of the substance by the brain of the fish reaching a maximum concentration of 98.9 ng g-1 8 h post treatment. The trend of the absorption of the drug in brain followed that of the blood. Concentration of the drug in the brain remained high at each sampling point over the 24 h duration of the experiment. In view of these findings, the need of study of the role of the blood-brain barrier and particularly the multidrug resistance mechanism in sea bream is outlined. © 2004 Elsevier B.V. All rights reserved. |
Galay-Burgos, M; Llewellyn, L; Mylonas, C C; Canario, A V M; Zanuy, S; Sweeney, G E Analysis of the Sox gene family in the European sea bass (Dicentrarchus labrax) Journal Article Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 137 (2), pp. 279–284, 2004, ISSN: 10964959, (Publisher: Elsevier Inc.). @article{galay-burgos_analysis_2004, title = {Analysis of the Sox gene family in the European sea bass (Dicentrarchus labrax)}, author = {M Galay-Burgos and L Llewellyn and C C Mylonas and A V M Canario and S Zanuy and G E Sweeney}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-1342321749&doi=10.1016%2fj.cbpc.2003.12.002&partnerID=40&md5=2aaa377948e005b5c31c7934d672e688}, doi = {10.1016/j.cbpc.2003.12.002}, issn = {10964959}, year = {2004}, date = {2004-01-01}, journal = {Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology}, volume = {137}, number = {2}, pages = {279--284}, abstract = {Sox (SRY-related genes containing a HMG box) genes encode a family of transcription factors that are involved in a variety of developmental processes including sex determination. Twenty Sox genes are present in the genomes of humans and mice, but far less is known about the Sox gene family in other vertebrate types. We have obtained clones representing the HMG boxes of twelve Sox genes from European sea bass (Dicentrarchus labrax), a fish species whose farming is complicated by a heavily skewed sex ratio, with between 70% and 99% of offspring typically being male. The cloned Sox genes are members of the SoxB, SoxC, SoxE and SoxF groups. Sequence analysis shows that some of the clones represent genes duplicated in sea bass with respect to the mammalian Sox gene family. © 2004 Elsevier Inc. All rights reserved.}, note = {Publisher: Elsevier Inc.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Sox (SRY-related genes containing a HMG box) genes encode a family of transcription factors that are involved in a variety of developmental processes including sex determination. Twenty Sox genes are present in the genomes of humans and mice, but far less is known about the Sox gene family in other vertebrate types. We have obtained clones representing the HMG boxes of twelve Sox genes from European sea bass (Dicentrarchus labrax), a fish species whose farming is complicated by a heavily skewed sex ratio, with between 70% and 99% of offspring typically being male. The cloned Sox genes are members of the SoxB, SoxC, SoxE and SoxF groups. Sequence analysis shows that some of the clones represent genes duplicated in sea bass with respect to the mammalian Sox gene family. © 2004 Elsevier Inc. All rights reserved. |
Rigos, G; Nengas, I; Alexis, M; Athanassopoulou, F Bioavailability of oxytetracycline in sea bass, Dicentrarchus labrax (L.) Journal Article Journal of Fish Diseases, 27 , pp. 119 – 122, 2004. @article{rigos_bioavailability_2004, title = {Bioavailability of oxytetracycline in sea bass, Dicentrarchus labrax (L.)}, author = {G Rigos and I Nengas and M Alexis and F Athanassopoulou}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-1542319008&partnerID=40&md5=2fce37501284273e5a1e06048b0e5bb3}, year = {2004}, date = {2004-01-01}, journal = {Journal of Fish Diseases}, volume = {27}, pages = {119 -- 122}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Papandroulakis, N; Kentouri, M; Divanach, P Biological performance of red porgy (Pagrus pagrus) larvae under intensive rearing conditions with the use of an automated feeding system Journal Article Aquaculture International, 12 , pp. 191 – 203, 2004. @article{papandroulakis_biological_2004, title = {Biological performance of red porgy (Pagrus pagrus) larvae under intensive rearing conditions with the use of an automated feeding system}, author = {N Papandroulakis and M Kentouri and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-3042549353&partnerID=40&md5=f683700be4763c4677be7bf71340bc32}, year = {2004}, date = {2004-01-01}, journal = {Aquaculture International}, volume = {12}, pages = {191 -- 203}, abstract = {Pagrus pagrus is one of the promising species for the industry in the Mediterranean but its rearing is still far from satisfactory. In the present work, the conditions and results of larval rearing with the use of an automatic feeding system are presented. Eight populations were reared for 20 days with the ’pseudo green water’ methodology in two successive trials. Larvae were fed enriched rotifers during the entire rearing period, a mixed diet of rotifers and Artemia (Instar I when larvae reached 5 mm in length, replaced by Instar II after larvae reached 5.5 mm) and a formulated diet (after day 16 post hatching). A computerized system for feeding management was used. A total of 388,000 eggs were incubated and after 20 days, 237,973 larvae (TL = 6.96 ± 0.17 mm) were produced with a survival rate of 61.4 ± 6.3%. Individuals grew in terms of wet weight with an exponential rate of 0.167 ± 0.008 daily (R2 = 0.983) with no variations between replicates. Mean individual daily consumption at first feeding (day 4-post hatching), was 0.20 ± 0.06 mg of food (0.03 ± 0.01 dry weight), that is, 180-300 rotifers, while on day 20, consumption increased by 10 (2.081 ± 0.106 mg and 0.276 ± 0.014 in wet and dry weight, respectively). When compared with sea bream, consumption was higher by approximately 3-4-fold. The mean food conversion ratio of the experimental period was 4. In comparison with sea bream larviculture, food consumption of red porgy is higher, and a different feeding strategy is required to satisfy the requirements of the larvae. © 2004 Kluwer Academic Publishers.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Pagrus pagrus is one of the promising species for the industry in the Mediterranean but its rearing is still far from satisfactory. In the present work, the conditions and results of larval rearing with the use of an automatic feeding system are presented. Eight populations were reared for 20 days with the ’pseudo green water’ methodology in two successive trials. Larvae were fed enriched rotifers during the entire rearing period, a mixed diet of rotifers and Artemia (Instar I when larvae reached 5 mm in length, replaced by Instar II after larvae reached 5.5 mm) and a formulated diet (after day 16 post hatching). A computerized system for feeding management was used. A total of 388,000 eggs were incubated and after 20 days, 237,973 larvae (TL = 6.96 ± 0.17 mm) were produced with a survival rate of 61.4 ± 6.3%. Individuals grew in terms of wet weight with an exponential rate of 0.167 ± 0.008 daily (R2 = 0.983) with no variations between replicates. Mean individual daily consumption at first feeding (day 4-post hatching), was 0.20 ± 0.06 mg of food (0.03 ± 0.01 dry weight), that is, 180-300 rotifers, while on day 20, consumption increased by 10 (2.081 ± 0.106 mg and 0.276 ± 0.014 in wet and dry weight, respectively). When compared with sea bream, consumption was higher by approximately 3-4-fold. The mean food conversion ratio of the experimental period was 4. In comparison with sea bream larviculture, food consumption of red porgy is higher, and a different feeding strategy is required to satisfy the requirements of the larvae. © 2004 Kluwer Academic Publishers. |
Halm, S; Martínez-Rodríguez, G; Rodríguez, L; Prat, F; Mylonas, C C; Carrillo, M; Zanuy, S Cloning, characterisation, and expression of three oestrogen receptors (ERα, ERβ1 and ERβ2) in the European sea bass, Dicentrarchus labrax Journal Article Molecular and Cellular Endocrinology, 223 (1-2), pp. 63–75, 2004, ISSN: 03037207. @article{halm_cloning_2004, title = {Cloning, characterisation, and expression of three oestrogen receptors (ERα, ERβ1 and ERβ2) in the European sea bass, Dicentrarchus labrax}, author = {S Halm and G Martínez-Rodríguez and L Rodríguez and F Prat and C C Mylonas and M Carrillo and S Zanuy}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-3342941616&doi=10.1016%2fj.mce.2004.05.009&partnerID=40&md5=46ce79c38bedf1df217fcb12ab9d17c8}, doi = {10.1016/j.mce.2004.05.009}, issn = {03037207}, year = {2004}, date = {2004-01-01}, journal = {Molecular and Cellular Endocrinology}, volume = {223}, number = {1-2}, pages = {63--75}, abstract = {Three oestrogen receptor [ER] subtypes have been described in teleost fish, namely ERα, and two ERβ subtypes, called ERβ1 and ERβ2 (or ERβ and ERγ in Atlantic croaker). Their expression during embryonic development and gonadal growth has evoked interest in their potential role in sexual differentiation and gonadal development in fish. We cloned three oestrogen receptors from adult liver (sb-ERα cDNA) and ovary (partial sb-ERβ1 and sb-ERβ2 cDNAs) of the European sea bass, and according to their phylogenetic relatedness to other ERs in teleosts, named them sea bass [sb-] ERα, ERβ1 and ERβ2. Deduced amino acid numbers for sb-ERα, sb-ERβ1 and sb-ERβ2 were 639, 517 and 608, respectively, representing in the case of sb-ERβ1 and sb-ERβ2 about 90% of the open reading frame. Highest amino acid identities were found for sb-ERα with eelpout ERα (88.7%), for sb-ERβ1 with Atlantic croaker ERγ (85.8%), and for sb-ERβ2 with Atlantic croaker ERβ (90.1%). Southern analysis confirmed that all three sea bass oestrogen receptors (sb-ERs) are the products of three distinct genes. In adult sea bass, ERα was predominantly expressed in liver and pituitary, while sb-ERβ1 and sb-ERβ2 were more ubiquitously expressed, with highest expression levels in pituitary. In a mixed-sex population of juvenile sea bass, sb-ERα expression was significantly elevated in gonads at 200 days posthatch (dph), while for sb-ERβ1 and sb-ERβ2 highest expression levels were observed in gonads at 250 dph. For sb-ERβ2, expression was also significantly higher in the brain at 250 dph. The cloning of these three ER subtypes in the European sea bass together with the results obtained on expression levels in adult and juvenile animals has given us the foundation to investigate their possible role in sexual differentiation and development in this species in future studies. © 2004 Elsevier Ireland Ltd. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Three oestrogen receptor [ER] subtypes have been described in teleost fish, namely ERα, and two ERβ subtypes, called ERβ1 and ERβ2 (or ERβ and ERγ in Atlantic croaker). Their expression during embryonic development and gonadal growth has evoked interest in their potential role in sexual differentiation and gonadal development in fish. We cloned three oestrogen receptors from adult liver (sb-ERα cDNA) and ovary (partial sb-ERβ1 and sb-ERβ2 cDNAs) of the European sea bass, and according to their phylogenetic relatedness to other ERs in teleosts, named them sea bass [sb-] ERα, ERβ1 and ERβ2. Deduced amino acid numbers for sb-ERα, sb-ERβ1 and sb-ERβ2 were 639, 517 and 608, respectively, representing in the case of sb-ERβ1 and sb-ERβ2 about 90% of the open reading frame. Highest amino acid identities were found for sb-ERα with eelpout ERα (88.7%), for sb-ERβ1 with Atlantic croaker ERγ (85.8%), and for sb-ERβ2 with Atlantic croaker ERβ (90.1%). Southern analysis confirmed that all three sea bass oestrogen receptors (sb-ERs) are the products of three distinct genes. In adult sea bass, ERα was predominantly expressed in liver and pituitary, while sb-ERβ1 and sb-ERβ2 were more ubiquitously expressed, with highest expression levels in pituitary. In a mixed-sex population of juvenile sea bass, sb-ERα expression was significantly elevated in gonads at 200 days posthatch (dph), while for sb-ERβ1 and sb-ERβ2 highest expression levels were observed in gonads at 250 dph. For sb-ERβ2, expression was also significantly higher in the brain at 250 dph. The cloning of these three ER subtypes in the European sea bass together with the results obtained on expression levels in adult and juvenile animals has given us the foundation to investigate their possible role in sexual differentiation and development in this species in future studies. © 2004 Elsevier Ireland Ltd. All rights reserved. |
Polymenakou, P N; Bertilsson, S; Tselepides, A; Stephanou, E G Comparison of microbial community composition from different sediments of the Eastern Mediterranean Sea using Ŧ-RFLP, DGGE and PLFA analysis Inproceedings Barcelona, Spain, 2004, (Publication Title: 37th CIESM Congress Type: Oral Presentation). @inproceedings{polymenakou_comparison_2004, title = {Comparison of microbial community composition from different sediments of the Eastern Mediterranean Sea using Ŧ-RFLP, DGGE and PLFA analysis}, author = {P N Polymenakou and S Bertilsson and A Tselepides and E G Stephanou}, year = {2004}, date = {2004-01-01}, address = {Barcelona, Spain}, note = {Publication Title: 37th CIESM Congress Type: Oral Presentation}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Tzovenis, I; Triantaphyllidis, G; Naihong, X; Chatzinikolaou, E; Papadopoulou, K; Xouri, G; Tafas, T Cryopreservation of marine microalgae and potential toxicity of cryoprotectants to the primary steps of the aquacultural food chain Journal Article Aquaculture, 230 (1-4), pp. 457–473, 2004, ISSN: 00448486, (Publisher: Elsevier). @article{tzovenis_cryopreservation_2004, title = {Cryopreservation of marine microalgae and potential toxicity of cryoprotectants to the primary steps of the aquacultural food chain}, author = {I Tzovenis and G Triantaphyllidis and X Naihong and E Chatzinikolaou and K Papadopoulou and G Xouri and T Tafas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0842310593&doi=10.1016%2fS0044-8486%2803%2900444-7&partnerID=40&md5=2ebddbf8a832f68bfc915dcafcbffe6e}, doi = {10.1016/S0044-8486(03)00444-7}, issn = {00448486}, year = {2004}, date = {2004-01-01}, journal = {Aquaculture}, volume = {230}, number = {1-4}, pages = {457--473}, abstract = {Cryopreservation, a technique of high potential for culture collections, might offer a solution for reliable supply of microalgae in aquaculture units. Marine microalgae used in aquaculture were cryopreserved under 4, -20 and -80°C using common cryoprotectants (methanol, dimethylsulfoxide (DMSO), propylene glycol and polyvinylpyrrolidone (PVP)) with promising results for Chlorella minutissima, Chlorella stigmatophora, Isochrysis galbana and Dunaliella tertiolecta. As cryoprotectants usually are toxic above certain concentrations and exposure time, and assuming that low amounts of cryoprotectants will remain in regenerated cultures, an experimental scheme was employed to explore the lower limits of safety for these algae and their primary consumers in hatchery food chains. Results showed that methanol was well tolerated by C. stigmatophora and D. tertiolecta up to a concentration of 1.6% (v/v) while I. galbana could not survive in culture at any concentration and C. minutissima exhibited some 30% of the control's yield at 0.2%. DMSO was highly tolerated up to 1.0% by all strains with the Chlorella strains surviving well up to 2%. Propylene glycol was not only tolerated up to 8% by Dunaliella but induced mixotrophic growth as well, while for Isochrysis it was lethal at any concentration. Among zooplanktonic consumers, brine shrimp Artemia nauplii could tolerate very high concentrations of the tested cryoprotectants, the rotifer Brachionus plicatilis was found sensitive to low amounts of PVP, while the nauplii of the shrimp Penaeus japonicus and the crab Eriocheir sinensis were in general very sensitive to all cryoprotectants and in several cases to much lower amounts than 1%. However, as long as the residues of cryoprotectants are kept below 1% in the regenerated cultures, there will be no problem with the animal consumers. © 2004 Elsevier B.V. All rights reserved.}, note = {Publisher: Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {article} } Cryopreservation, a technique of high potential for culture collections, might offer a solution for reliable supply of microalgae in aquaculture units. Marine microalgae used in aquaculture were cryopreserved under 4, -20 and -80°C using common cryoprotectants (methanol, dimethylsulfoxide (DMSO), propylene glycol and polyvinylpyrrolidone (PVP)) with promising results for Chlorella minutissima, Chlorella stigmatophora, Isochrysis galbana and Dunaliella tertiolecta. As cryoprotectants usually are toxic above certain concentrations and exposure time, and assuming that low amounts of cryoprotectants will remain in regenerated cultures, an experimental scheme was employed to explore the lower limits of safety for these algae and their primary consumers in hatchery food chains. Results showed that methanol was well tolerated by C. stigmatophora and D. tertiolecta up to a concentration of 1.6% (v/v) while I. galbana could not survive in culture at any concentration and C. minutissima exhibited some 30% of the control's yield at 0.2%. DMSO was highly tolerated up to 1.0% by all strains with the Chlorella strains surviving well up to 2%. Propylene glycol was not only tolerated up to 8% by Dunaliella but induced mixotrophic growth as well, while for Isochrysis it was lethal at any concentration. Among zooplanktonic consumers, brine shrimp Artemia nauplii could tolerate very high concentrations of the tested cryoprotectants, the rotifer Brachionus plicatilis was found sensitive to low amounts of PVP, while the nauplii of the shrimp Penaeus japonicus and the crab Eriocheir sinensis were in general very sensitive to all cryoprotectants and in several cases to much lower amounts than 1%. However, as long as the residues of cryoprotectants are kept below 1% in the regenerated cultures, there will be no problem with the animal consumers. © 2004 Elsevier B.V. All rights reserved. |
Company, JB; Maiorano, P; Tselepides, A; Politou, C -Y; Plaiti, W; Rollant, G; Sardá, F Deep-sea decapod crustaceans in the western and central Mediterranean Sea: preliminary aspect of species distribution, biomass and population structure. Journal Article Scientia Marina, 68 , pp. 73–86, 2004. @article{company_deep-sea_2004, title = {Deep-sea decapod crustaceans in the western and central Mediterranean Sea: preliminary aspect of species distribution, biomass and population structure.}, author = {JB Company and P Maiorano and A Tselepides and C -Y Politou and W Plaiti and G Rollant and F Sardá}, year = {2004}, date = {2004-01-01}, journal = {Scientia Marina}, volume = {68}, pages = {73--86}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Tyrpenou, A E; Rigos, G Determination of oxolinic acid residues in gilthead seabream (Sparus aurata) muscle tissue and plasma by high-performance liquid chromatography Journal Article Chromatographia, 60 , pp. 657 – 661, 2004. @article{tyrpenou_determination_2004, title = {Determination of oxolinic acid residues in gilthead seabream (Sparus aurata) muscle tissue and plasma by high-performance liquid chromatography}, author = {A E Tyrpenou and G Rigos}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-12144273132&partnerID=40&md5=5b05f1505cf35b3cf48c9dd1fbc39a35}, year = {2004}, date = {2004-01-01}, journal = {Chromatographia}, volume = {60}, pages = {657 -- 661}, abstract = {A high-performance liquid chromatographic method for the determination of oxolinic acid (OA) residues in muscle tissue and plasma of the cultured fish gilthead seabream (Sparus aurata L.), is described. OA was extracted with ethyl acetate and after centrifugation the combined extracts were evaporated. To the remaining residue 1 mL of the mobile phase was added and the extract was partitioned with n-pentane which then was rejected by aspiration. OA was chromatographed on a Zorbax®SB-C18 column at 50°C and detected by fluorescence detection at λex 327 nm and λem 369 nm. The mobile phase was a mixture of 0.1% trifluoroacetic acid (v/v) pH 2.0 and acetonitrile-methanol 3:2 (v/v) in a combination of 50:50 (v/v) and a flow rate of 1.0 mL min-1, delivered isocratically. Method mean recovery (R%) achieved was 73.7 ± 4.4% (mean ± SD) for blank fortified samples (n = 4) range at 50, 100 and 200 μg kg-1 with a RSD = 3.3%. The limit of detection (LOD) was 2.0 μg kg-1 oxolinic acid in muscle tissue and plasma and the limit of quantification (LOQ) was 5.0 μg kg-1. The method is fast and suitable to be used with safety and accuracy for the control of OA residues in cultured seabreams and a trained analyst could carry out ready for chromatography more than 50 samples per working day.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A high-performance liquid chromatographic method for the determination of oxolinic acid (OA) residues in muscle tissue and plasma of the cultured fish gilthead seabream (Sparus aurata L.), is described. OA was extracted with ethyl acetate and after centrifugation the combined extracts were evaporated. To the remaining residue 1 mL of the mobile phase was added and the extract was partitioned with n-pentane which then was rejected by aspiration. OA was chromatographed on a Zorbax®SB-C18 column at 50°C and detected by fluorescence detection at λex 327 nm and λem 369 nm. The mobile phase was a mixture of 0.1% trifluoroacetic acid (v/v) pH 2.0 and acetonitrile-methanol 3:2 (v/v) in a combination of 50:50 (v/v) and a flow rate of 1.0 mL min-1, delivered isocratically. Method mean recovery (R%) achieved was 73.7 ± 4.4% (mean ± SD) for blank fortified samples (n = 4) range at 50, 100 and 200 μg kg-1 with a RSD = 3.3%. The limit of detection (LOD) was 2.0 μg kg-1 oxolinic acid in muscle tissue and plasma and the limit of quantification (LOQ) was 5.0 μg kg-1. The method is fast and suitable to be used with safety and accuracy for the control of OA residues in cultured seabreams and a trained analyst could carry out ready for chromatography more than 50 samples per working day. |
Chistiakov, D A; Hellemans, B; Tsigenopoulos, C S; Law, A S; Bartley, N; Bertotto, D; Libertini, A; Kotoulas, G; Haley, C S; Volckaert, F A M Development and linkage relationships for new microsatellite markers of the sea bass (Dicentrarchus labrax L.) Journal Article Animal Genetics, 35 (1), pp. 53–57, 2004, ISSN: 02689146. @article{chistiakov_development_2004, title = {Development and linkage relationships for new microsatellite markers of the sea bass (Dicentrarchus labrax L.)}, author = {D A Chistiakov and B Hellemans and C S Tsigenopoulos and A S Law and N Bartley and D Bertotto and A Libertini and G Kotoulas and C S Haley and F A M Volckaert}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-10744228402&doi=10.1046%2fj.1365-2052.2003.01076.x&partnerID=40&md5=04f787b68dff2aecc25e57226b021721}, doi = {10.1046/j.1365-2052.2003.01076.x}, issn = {02689146}, year = {2004}, date = {2004-01-01}, journal = {Animal Genetics}, volume = {35}, number = {1}, pages = {53--57}, abstract = {Twenty-eight polymorphic microsatellites were isolated from the sea bass, Dicentrarchus labrax, using a microsatellite enrichment protocol and selective hybridization with oligonucleotide probes. Analysis for these markers and 11 recently described microsatellites of D. labrax found linkage between 26 loci and revealed eight linkage groups.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Twenty-eight polymorphic microsatellites were isolated from the sea bass, Dicentrarchus labrax, using a microsatellite enrichment protocol and selective hybridization with oligonucleotide probes. Analysis for these markers and 11 recently described microsatellites of D. labrax found linkage between 26 loci and revealed eight linkage groups. |
Martinez, Brotons J; Chatzifotis, S; Divanach, P; Takeuchi, T Effect of dietary taurine supplementation on growth performance and feed selection of sea bass Dicentrarchus labrax fry fed with demand-feeders Journal Article Fisheries Science, 70 (1), pp. 74–79, 2004, ISSN: 09199268. @article{brotons_martinez_effect_2004, title = {Effect of dietary taurine supplementation on growth performance and feed selection of sea bass Dicentrarchus labrax fry fed with demand-feeders}, author = {J Brotons Martinez and S Chatzifotis and P Divanach and T Takeuchi}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-1542381102&doi=10.1111%2fj.1444-2906.2003.00773.x&partnerID=40&md5=3c8ecc80e8dfafd48e7224dbfaacd7ea}, doi = {10.1111/j.1444-2906.2003.00773.x}, issn = {09199268}, year = {2004}, date = {2004-01-01}, journal = {Fisheries Science}, volume = {70}, number = {1}, pages = {74--79}, abstract = {Two series of experiments were conducted for the evaluation of the effect of dietary taurine supplementation on survival, growth performance and feed preference of sea bass Dicentrarchus labrax fry when fed with demand-feeders a fish/soybean meal-based diet. In the first experiment, fish were imposed one of four diets supplemented with taurine (0, 0.1, 0.2 or 0.3% taurine on a dry weight basis), while in the second they had simultaneous free access to the four diets supplemented with taurine. When not allowed to choose, fish exhibited better specific growth rate of 3.2 and 3.2% per day with diets supplemented with 0.2 and 0.3% taurine than those of 2.3 and 2.2% per day of O and 0.1% taurine, respectively. When fish were free to access the diets, they selected more actively the 0.2 and 0.3% taurine-supplemented diets than the 0 and 0.1% taurine. As survival was not significantly different among trials, the results indicate that sea bass fry require a 0.2% taurine in the diet for better growth when fish meal and soybean meal are the primary sources of protein. The positive correlation between growth performance and nutrient preference is discussed as a method for improvement of fry food formulation.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Two series of experiments were conducted for the evaluation of the effect of dietary taurine supplementation on survival, growth performance and feed preference of sea bass Dicentrarchus labrax fry when fed with demand-feeders a fish/soybean meal-based diet. In the first experiment, fish were imposed one of four diets supplemented with taurine (0, 0.1, 0.2 or 0.3% taurine on a dry weight basis), while in the second they had simultaneous free access to the four diets supplemented with taurine. When not allowed to choose, fish exhibited better specific growth rate of 3.2 and 3.2% per day with diets supplemented with 0.2 and 0.3% taurine than those of 2.3 and 2.2% per day of O and 0.1% taurine, respectively. When fish were free to access the diets, they selected more actively the 0.2 and 0.3% taurine-supplemented diets than the 0 and 0.1% taurine. As survival was not significantly different among trials, the results indicate that sea bass fry require a 0.2% taurine in the diet for better growth when fish meal and soybean meal are the primary sources of protein. The positive correlation between growth performance and nutrient preference is discussed as a method for improvement of fry food formulation. |
Mandalakis, M; Zebühr, Y; Gustafsson, Ö Efficient isolation of polyaromatic fraction from aliphatic compounds in complex extracts using dimethylformamide-pentane partitionings Journal Article Journal of Chromatography A, 1041 (1-2), pp. 111–117, 2004, ISSN: 00219673. @article{mandalakis_efficient_2004, title = {Efficient isolation of polyaromatic fraction from aliphatic compounds in complex extracts using dimethylformamide-pentane partitionings}, author = {M Mandalakis and Y Zebühr and Ö Gustafsson}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-2942567872&doi=10.1016%2fj.chroma.2004.04.038&partnerID=40&md5=e5e205d42a77a25eb4e3a323824426fd}, doi = {10.1016/j.chroma.2004.04.038}, issn = {00219673}, year = {2004}, date = {2004-01-01}, journal = {Journal of Chromatography A}, volume = {1041}, number = {1-2}, pages = {111--117}, abstract = {A liquid-liquid partitioning method was optimized for the rapid and quantitative separation of polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) from aliphatic hydrocarbons in complex primary extracts. This technique was based on the selective extraction of PAHs and PCBs from an aliphatic solvent into dimethylformamide (DMF). Partition experiments demonstrated that the optimal performance was achieved with a DMF (5% H 2O)-n-pentane binary system. The optimized application of two consecutive DMF (5% H2O)-n-pentane treatments to extracts from two different polluted sediments facilitated the elimination of alkanes and unresolved complex mixture by more than 94% while the average recoveries of spiked deuterated-PAHs and 13C labeled PCBs ranged from 84 to 94 and 75 to 96%, respectively. © 2004 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A liquid-liquid partitioning method was optimized for the rapid and quantitative separation of polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) from aliphatic hydrocarbons in complex primary extracts. This technique was based on the selective extraction of PAHs and PCBs from an aliphatic solvent into dimethylformamide (DMF). Partition experiments demonstrated that the optimal performance was achieved with a DMF (5% H 2O)-n-pentane binary system. The optimized application of two consecutive DMF (5% H2O)-n-pentane treatments to extracts from two different polluted sediments facilitated the elimination of alkanes and unresolved complex mixture by more than 94% while the average recoveries of spiked deuterated-PAHs and 13C labeled PCBs ranged from 84 to 94 and 75 to 96%, respectively. © 2004 Elsevier B.V. All rights reserved. |
Mylonas, C C; Papadaki, M; Pavlidis, M; Divanach, P Evaluation of egg production and quality in the Mediterranean red porgy (Pagrus pagrus) during two consecutive spawning seasons Journal Article Aquaculture, 232 (1-4), pp. 637–649, 2004, ISSN: 00448486. @article{mylonas_evaluation_2004, title = {Evaluation of egg production and quality in the Mediterranean red porgy (Pagrus pagrus) during two consecutive spawning seasons}, author = {C C Mylonas and M Papadaki and M Pavlidis and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-1542321216&doi=10.1016%2fS0044-8486%2803%2900534-9&partnerID=40&md5=bb798427551c7d2bdbf2d0812cf57e45}, doi = {10.1016/S0044-8486(03)00534-9}, issn = {00448486}, year = {2004}, date = {2004-01-01}, journal = {Aquaculture}, volume = {232}, number = {1-4}, pages = {637--649}, abstract = {Egg production from two broodstocks of captive-reared red porgy (Pagrus pagrus) was monitored for two consecutive seasons (2001 and 2002). Spawning lasted from January to April-May under water temperature between 15.8 and 19.2°C. Spawning was mostly daily, though over the course of the two spawning seasons, the mean interval was 1.5-1.2 days spawn-1. There was a large variation in daily fecundity without a consistent trend, except for a slight peak in the middle of the spawning season. Mean (S.E.M.) annual relative fecundity ranged between 408,300 (95,800) and 442,500 (180,800) eggs kg-1 year-1. Mean fertilization success increased significantly from 36.9 (2.0)% in 2001 to 68.9 (2.5)% in 2002, resulting in mean annual viable egg production of 174,800 (87,600) and 301,500 (99,800) eggs kg-1, respectively. There were no significant differences between the 2 years in other egg quality parameters, which ranged between 74.5 and 79. 3% 1-day embryo survival, 81.6-85.5% hatching success and 76.7-78.8% 5-day larval survival. Of the relations examined, fertilization % had a significantly positive correlation with 1-day embryo survival, which in turn was significantly correlated with hatching %. Larval survival was further correlated with hatching %. These data suggest that decisions on whether to proceed with the incubation of a batch of eggs and rearing of the larvae can be made within 1 day from egg collection, before much effort is invested by the hatchery. The present study presents further information on the spawning kinetics, and egg production and quality characteristics of the Mediterranean red porgy in culture, and demonstrates the suitability of this species for reproduction in captivity. © 2004 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Egg production from two broodstocks of captive-reared red porgy (Pagrus pagrus) was monitored for two consecutive seasons (2001 and 2002). Spawning lasted from January to April-May under water temperature between 15.8 and 19.2°C. Spawning was mostly daily, though over the course of the two spawning seasons, the mean interval was 1.5-1.2 days spawn-1. There was a large variation in daily fecundity without a consistent trend, except for a slight peak in the middle of the spawning season. Mean (S.E.M.) annual relative fecundity ranged between 408,300 (95,800) and 442,500 (180,800) eggs kg-1 year-1. Mean fertilization success increased significantly from 36.9 (2.0)% in 2001 to 68.9 (2.5)% in 2002, resulting in mean annual viable egg production of 174,800 (87,600) and 301,500 (99,800) eggs kg-1, respectively. There were no significant differences between the 2 years in other egg quality parameters, which ranged between 74.5 and 79. 3% 1-day embryo survival, 81.6-85.5% hatching success and 76.7-78.8% 5-day larval survival. Of the relations examined, fertilization % had a significantly positive correlation with 1-day embryo survival, which in turn was significantly correlated with hatching %. Larval survival was further correlated with hatching %. These data suggest that decisions on whether to proceed with the incubation of a batch of eggs and rearing of the larvae can be made within 1 day from egg collection, before much effort is invested by the hatchery. The present study presents further information on the spawning kinetics, and egg production and quality characteristics of the Mediterranean red porgy in culture, and demonstrates the suitability of this species for reproduction in captivity. © 2004 Elsevier B.V. All rights reserved. |
Chatzifotis, S; Muje, P; Pavlidis, M; Ågren, J; Paalavuo, M; Mölsä, H Evolution of tissue composition and serum metabolites during gonadal development in the common dentex (Dentex dentex) Journal Article Aquaculture, 236 (1-4), pp. 557–573, 2004, ISSN: 00448486. @article{chatzifotis_evolution_2004, title = {Evolution of tissue composition and serum metabolites during gonadal development in the common dentex (Dentex dentex)}, author = {S Chatzifotis and P Muje and M Pavlidis and J Ågren and M Paalavuo and H Mölsä}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-2442560250&doi=10.1016%2fj.aquaculture.2003.12.004&partnerID=40&md5=e8411aeb1e2be9cbf5f66ae7979b840e}, doi = {10.1016/j.aquaculture.2003.12.004}, issn = {00448486}, year = {2004}, date = {2004-01-01}, journal = {Aquaculture}, volume = {236}, number = {1-4}, pages = {557--573}, abstract = {This study describes the evolution of tissue composition and serum metabolites of common dentex (Dentex dentex) in different maturity stages. During maturation, diet and perivisceral fat provide the materials needed for the development of gonads. Female and male fish had higher total proteins, total fat, triglycerides (TG) and cholesterol in their serum, at spawning and spermiation, respectively. The examination of dentex tissues (muscle, liver and gonad) revealed no statistically significant differences (Ptextless0.05) in energy density and lipid content during different maturity stages and there was no conclusive evidence that energy density and lipid content differ between sexes. In muscle, liver and gonad triglycerides fraction, the proportion of saturated fatty acids (FA) was greater than the proportion of polyunsaturated fatty acids (PUFA), whereas the reverse observation was made (with the exception of liver) in the polar lipid (PL) fraction of the above tissues. In muscle and liver, the main polyunsaturated fatty acids (n-3 PUFA) were docosahexaenoic (DHA, 22:6n-3) and eicosapentaenoic (EPA, 20:5n-3) acids. The main n-6 polyunsaturated fatty acids were linoleic (18:2n-6) and arachidonic (20:4n-6) acids. Palmitic (16:0) and oleic (18:1n-9) acids were the main saturated fatty acids and monoenes, respectively. The relative concentration of the major fatty acids varied seasonally and a pattern can be seen in both mature and immature dentex. In the PL fraction of muscle, higher values of total saturated and n-3 fatty acids, palmitic acid and DHA were obtained during summer in comparison to winter samplings; this trend was also shown by the total saturated fatty acids in the TG fraction. © 2003 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study describes the evolution of tissue composition and serum metabolites of common dentex (Dentex dentex) in different maturity stages. During maturation, diet and perivisceral fat provide the materials needed for the development of gonads. Female and male fish had higher total proteins, total fat, triglycerides (TG) and cholesterol in their serum, at spawning and spermiation, respectively. The examination of dentex tissues (muscle, liver and gonad) revealed no statistically significant differences (Ptextless0.05) in energy density and lipid content during different maturity stages and there was no conclusive evidence that energy density and lipid content differ between sexes. In muscle, liver and gonad triglycerides fraction, the proportion of saturated fatty acids (FA) was greater than the proportion of polyunsaturated fatty acids (PUFA), whereas the reverse observation was made (with the exception of liver) in the polar lipid (PL) fraction of the above tissues. In muscle and liver, the main polyunsaturated fatty acids (n-3 PUFA) were docosahexaenoic (DHA, 22:6n-3) and eicosapentaenoic (EPA, 20:5n-3) acids. The main n-6 polyunsaturated fatty acids were linoleic (18:2n-6) and arachidonic (20:4n-6) acids. Palmitic (16:0) and oleic (18:1n-9) acids were the main saturated fatty acids and monoenes, respectively. The relative concentration of the major fatty acids varied seasonally and a pattern can be seen in both mature and immature dentex. In the PL fraction of muscle, higher values of total saturated and n-3 fatty acids, palmitic acid and DHA were obtained during summer in comparison to winter samplings; this trend was also shown by the total saturated fatty acids in the TG fraction. © 2003 Elsevier B.V. All rights reserved. |
Papandroulakis, N; Suquet, M; Spedicato, M T; Machias, A; Fauvel, C; Divanach, P Feeding rates, growth performance and gametogenesis of wreckfish (Polyprion americanus) kept in captivity Journal Article Aquaculture International, 12 , pp. 395 – 407, 2004. @article{papandroulakis_feeding_2004, title = {Feeding rates, growth performance and gametogenesis of wreckfish (Polyprion americanus) kept in captivity}, author = {N Papandroulakis and M Suquet and M T Spedicato and A Machias and C Fauvel and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33749494732&partnerID=40&md5=452a6a205ec500f8fdcd8736ff3a50f1}, year = {2004}, date = {2004-01-01}, journal = {Aquaculture International}, volume = {12}, pages = {395 -- 407}, abstract = {Wreckfish (Polyprion americanus) is a panoceanic, antitropical distributed species. Despite the high market price, high growth rate, good quality flesh and the potential for aquaculture, research data are scarce. In this study, the growth performance and food intake was investigated in juvenile fish, while gametogenesis was studied in adult fish. Three groups were created from 19 individuals, which were collected during neustonic sampling, using fish aggregating devices, in association with floating objects and during tuna fishing with drift nets from Greece (Crete), Italy (Ionian Sea) and the Atlantic coast of France from September 1999 to March 2001. All individuals (initial weight between 0.5 g and 4.5kg) were reared in aquaculture facilities so as to monitor growth performance and food intake. Food (de-frozen fish - bogue, mackerel and squid - or moist pellets) was delivered ad libitum. Total length and wet weight were measured regularly. Daily food intake, the food conversion index and daily growth rate were calculated. Individuals, aging 0+, grew exceptionally fast, reaching 2.0kg (total length 50cm) in 12 months and 5.0kg (total length 65cm) in 24, while at later stages, the growth rate decreased and depended on the water temperature during rearing. Food consumption varied during the rearing period according to the developmental stage and the rearing conditions. The food conversion ratio ranged between 0.9 and 2.5 on dry weight basis during the period of the study. Gametogenesis was monitored in a separate stock of 15 individuals (11.7 ± 3.7 kg) by biopsis while blood samples were taken for steroid estimation. Increased oocyte diameter was observed in females from August to January, while plasma estradiol levels also increased from October to March. However, neither egg or sperm were collected from the stock. Young wreckfish presented a high growth rate in captivity, thus supporting the feasibility of the species for rearing. Further research, however, is required for the reproduction of the species. © 2004 Kluwer Academic Publishers.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Wreckfish (Polyprion americanus) is a panoceanic, antitropical distributed species. Despite the high market price, high growth rate, good quality flesh and the potential for aquaculture, research data are scarce. In this study, the growth performance and food intake was investigated in juvenile fish, while gametogenesis was studied in adult fish. Three groups were created from 19 individuals, which were collected during neustonic sampling, using fish aggregating devices, in association with floating objects and during tuna fishing with drift nets from Greece (Crete), Italy (Ionian Sea) and the Atlantic coast of France from September 1999 to March 2001. All individuals (initial weight between 0.5 g and 4.5kg) were reared in aquaculture facilities so as to monitor growth performance and food intake. Food (de-frozen fish - bogue, mackerel and squid - or moist pellets) was delivered ad libitum. Total length and wet weight were measured regularly. Daily food intake, the food conversion index and daily growth rate were calculated. Individuals, aging 0+, grew exceptionally fast, reaching 2.0kg (total length 50cm) in 12 months and 5.0kg (total length 65cm) in 24, while at later stages, the growth rate decreased and depended on the water temperature during rearing. Food consumption varied during the rearing period according to the developmental stage and the rearing conditions. The food conversion ratio ranged between 0.9 and 2.5 on dry weight basis during the period of the study. Gametogenesis was monitored in a separate stock of 15 individuals (11.7 ± 3.7 kg) by biopsis while blood samples were taken for steroid estimation. Increased oocyte diameter was observed in females from August to January, while plasma estradiol levels also increased from October to March. However, neither egg or sperm were collected from the stock. Young wreckfish presented a high growth rate in captivity, thus supporting the feasibility of the species for rearing. Further research, however, is required for the reproduction of the species. © 2004 Kluwer Academic Publishers. |
Mylonas, C C; Papandroulakis, N; Smboukis, A; Papadaki, M; Divanach, P Induction of spawning of cultured greater amberjack (Seriola dumerili) using GnRHa implants Journal Article Aquaculture, 237 (1-4), pp. 141–154, 2004, ISSN: 00448486. @article{mylonas_induction_2004, title = {Induction of spawning of cultured greater amberjack (Seriola dumerili) using GnRHa implants}, author = {C C Mylonas and N Papandroulakis and A Smboukis and M Papadaki and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-3142566876&doi=10.1016%2fj.aquaculture.2004.04.015&partnerID=40&md5=1d5f2c1607615bf23df2ad0e72aaa783}, doi = {10.1016/j.aquaculture.2004.04.015}, issn = {00448486}, year = {2004}, date = {2004-01-01}, journal = {Aquaculture}, volume = {237}, number = {1-4}, pages = {141--154}, abstract = {The objective of the current study was to examine the possibility of maturing Mediterranean greater amberjack (Seriola dumerili) in culture at two different facilities in Greece and inducing spawning using gonadotropin releasing-hormone agonist (GnRHa) implants. Males in both stocks (n=8) were in spermiating condition in mid June, though sperm motility was low ranging between 5% and 30%, while motility duration ranged between 2.1 and 2.5 min. Females of the first stock (n=4) at the same time had ovaries with either primary oocytes or early vitellogenic oocytes at advanced stages of atresia, indicating a failure in oogenesis. On the contrary, the only female of the second stock contained oocytes at all stages of oogenesis, including early final oocyte maturation (FOM; oocyte diameter 650 μm). Implantation with a GnRHa implant induced the first spawn about 36 h later. In the absence of another spawn, a gonadal biopsy was taken 15 days later, indicating the existence of more oocytes at post vitellogenesis and some with almost complete lipid droplet coalescence. A second GnRHa implantation at this time resulted in consecutive egg releases after 36 h, 4 and 5 days, with fertilized eggs only at 36 h and 5 days. The study underlines the existence of important reproductive dysfunctions of greater amberjack in captivity, and demonstrates that GnRHa implants can be used for the induction of multiple spawns of viable eggs. © 2004 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The objective of the current study was to examine the possibility of maturing Mediterranean greater amberjack (Seriola dumerili) in culture at two different facilities in Greece and inducing spawning using gonadotropin releasing-hormone agonist (GnRHa) implants. Males in both stocks (n=8) were in spermiating condition in mid June, though sperm motility was low ranging between 5% and 30%, while motility duration ranged between 2.1 and 2.5 min. Females of the first stock (n=4) at the same time had ovaries with either primary oocytes or early vitellogenic oocytes at advanced stages of atresia, indicating a failure in oogenesis. On the contrary, the only female of the second stock contained oocytes at all stages of oogenesis, including early final oocyte maturation (FOM; oocyte diameter 650 μm). Implantation with a GnRHa implant induced the first spawn about 36 h later. In the absence of another spawn, a gonadal biopsy was taken 15 days later, indicating the existence of more oocytes at post vitellogenesis and some with almost complete lipid droplet coalescence. A second GnRHa implantation at this time resulted in consecutive egg releases after 36 h, 4 and 5 days, with fertilized eggs only at 36 h and 5 days. The study underlines the existence of important reproductive dysfunctions of greater amberjack in captivity, and demonstrates that GnRHa implants can be used for the induction of multiple spawns of viable eggs. © 2004 Elsevier B.V. All rights reserved. |
Zampicinini, G; Blinov, A; Cervella, P; Guryev, V; Sella, G Genome, 47 , pp. 1154–1163, 2004. @article{zampicinini_insertional_2004, title = {Insertional polymorphism of a non-LTR mobile element (NLRCth1) in European populations of Chironomus riparius (Diptera, Chironomidae) as detected by transposon insertion display}, author = {G Zampicinini and A Blinov and P Cervella and V Guryev and G Sella}, url = {http://www.nrcresearchpress.com/doi/abs/10.1139/g04-066}, doi = {10.1139/g04-066}, year = {2004}, date = {2004-01-01}, journal = {Genome}, volume = {47}, pages = {1154--1163}, abstract = {The midge Chironomus riparius is distributed all over the Palearctic region and is well characterized both at the morphological and cytogenetic levels. Here we describe a population study based on the insertional polymorphism of the retroposon NLRCth1, by means of a S-SAP (sequence-specific amplification polymorphism) derived technique (transposon insertion display; TID). While a previous study of allozyme polymorphism in Russian samples showed little variability, all the amplicons we identified are polymorphic. Genetic distances between 6 natural populations were calculated according to Nei and did not show a positive correlation with geographic distances. The genetic diversity detected among individuals of a given population was one order of magnitude higher than that among populations. However, the value of ΦST was significant (p < 0.001) and indicates that natural populations are more genetically differentiated than random samples of individuals. © 2004 NRC Canada.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The midge Chironomus riparius is distributed all over the Palearctic region and is well characterized both at the morphological and cytogenetic levels. Here we describe a population study based on the insertional polymorphism of the retroposon NLRCth1, by means of a S-SAP (sequence-specific amplification polymorphism) derived technique (transposon insertion display; TID). While a previous study of allozyme polymorphism in Russian samples showed little variability, all the amplicons we identified are polymorphic. Genetic distances between 6 natural populations were calculated according to Nei and did not show a positive correlation with geographic distances. The genetic diversity detected among individuals of a given population was one order of magnitude higher than that among populations. However, the value of ΦST was significant (p < 0.001) and indicates that natural populations are more genetically differentiated than random samples of individuals. © 2004 NRC Canada. |
Atienza, M T; Chatzifotis, S; Divanach, P Macronutrient selection by sharp snout seabream (Diplodus puntazzo) Journal Article Aquaculture, 232 (1-4), pp. 481–491, 2004, ISSN: 00448486. @article{atienza_macronutrient_2004, title = {Macronutrient selection by sharp snout seabream (Diplodus puntazzo)}, author = {M T Atienza and S Chatzifotis and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-1542321232&doi=10.1016%2fS0044-8486%2803%2900468-X&partnerID=40&md5=7f2fd0488cac53aa1a23cefd86f2ffa5}, doi = {10.1016/S0044-8486(03)00468-X}, issn = {00448486}, year = {2004}, date = {2004-01-01}, journal = {Aquaculture}, volume = {232}, number = {1-4}, pages = {481--491}, abstract = {This study is concerned with the investigation of feed preference in juvenile sharp snout seabream (Diplodus puntazzo). Three isoenergetic diets, high protein-low fat (HPLF), medium protein and fat (MPMF) and high fat-low protein (LPHF) were tested using six feeding regimes. Three groups of fish were used as controls to test the diets independently, while to the other three, a different diet combination was provided (HPLF-MPMF, MPMF-LPHF and HPLF-LPHF). Diets were provided by means of one or two self-feeders and fish had continuous access to one or two diets. Fish had an initial weight of 11.1±3.8 g (mean±S.D.) and after 45 days of feeding, no significant differences (Ptextless0.05) was observed in specific growth rate (SGR), feed conversion ratio (FCR) and protein efficiency ratio (PER) among different groups. Fish on HPLF-MPMF and HPLF-LPHF feeding regimes showed the highest growth rate of 1.96% per day and the lowest feed conversion ratio of 1.09, respectively. The result obtained showed that although there was different availability of diets among groups, macronutrient consumption appears to converge suggesting a target intake (47% protein and 10% fat content). The protein content of the diet influenced feed consumption, growth and feed conversion ratio. © 2004 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study is concerned with the investigation of feed preference in juvenile sharp snout seabream (Diplodus puntazzo). Three isoenergetic diets, high protein-low fat (HPLF), medium protein and fat (MPMF) and high fat-low protein (LPHF) were tested using six feeding regimes. Three groups of fish were used as controls to test the diets independently, while to the other three, a different diet combination was provided (HPLF-MPMF, MPMF-LPHF and HPLF-LPHF). Diets were provided by means of one or two self-feeders and fish had continuous access to one or two diets. Fish had an initial weight of 11.1±3.8 g (mean±S.D.) and after 45 days of feeding, no significant differences (Ptextless0.05) was observed in specific growth rate (SGR), feed conversion ratio (FCR) and protein efficiency ratio (PER) among different groups. Fish on HPLF-MPMF and HPLF-LPHF feeding regimes showed the highest growth rate of 1.96% per day and the lowest feed conversion ratio of 1.09, respectively. The result obtained showed that although there was different availability of diets among groups, macronutrient consumption appears to converge suggesting a target intake (47% protein and 10% fat content). The protein content of the diet influenced feed consumption, growth and feed conversion ratio. © 2004 Elsevier B.V. All rights reserved. |
Papandroulakis, N; Kentouri, M; Maingot, E; Divanach, P Mesocosm: A reliable technology for larval rearing of Diplodus puntazzo and Diplodus sargus sargus Journal Article Aquaculture International, 12 , pp. 345 – 355, 2004. @article{papandroulakis_mesocosm_2004, title = {Mesocosm: A reliable technology for larval rearing of Diplodus puntazzo and Diplodus sargus sargus}, author = {N Papandroulakis and M Kentouri and E Maingot and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33644987838&partnerID=40&md5=76b681983b3ee6f2281fdabaab3a4b3d}, year = {2004}, date = {2004-01-01}, journal = {Aquaculture International}, volume = {12}, pages = {345 -- 355}, abstract = {Species diversification is today considered as a major issue for the sustainable development of the Mediterranean aquaculture. For successful propagation of any species however, larval rearing is considered a bottleneck and therefore the development of appropriate tools is essential. Mesocosm is a semi-intensive technology that facilitates larval rearing of several species integrating principles of both intensive and extensive aquaculture, which solves biological problems and many of their technical, human and economical consequences. The extensive (and now even the semi-extensive) strategy is used in the most critical segments of the rearing process during the early developmental stages, when larvae are still extremely weak, sensitive to intensive environment, easily stressed and difficult to feed. The intensive strategy is used as soon as larvae are considered mature enough to be reared easily using classical methods. The technology was used for the rearing of two species, with potential for aquaculture, the sharpsnout seabream (Diplodus puntazzo) and the white seabream (Diplodus sargus sargus). Three groups of each species were monitored for a period of 50-70 days post hatching. Survival for both species was about 54% at the end of the trials. Sharpsnout seabream larvae reached 19.6 ± 0.9 mm total length and 107.2±31.9mg body weight 50 days post hatching. White seabream larvae 60 days post hatching reached 32.7 ± 2.7 mm total length and 450±70mg body weight. In order to verify the economical viability of the technology, the individual production cost for each species was estimated and reached €0.027 for white seabream and €0.043 for sharpsnout seabream. Results indicate the reliability of the technology for the larval rearing of the two species. © 2004 Kluwer Academic Publishers.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Species diversification is today considered as a major issue for the sustainable development of the Mediterranean aquaculture. For successful propagation of any species however, larval rearing is considered a bottleneck and therefore the development of appropriate tools is essential. Mesocosm is a semi-intensive technology that facilitates larval rearing of several species integrating principles of both intensive and extensive aquaculture, which solves biological problems and many of their technical, human and economical consequences. The extensive (and now even the semi-extensive) strategy is used in the most critical segments of the rearing process during the early developmental stages, when larvae are still extremely weak, sensitive to intensive environment, easily stressed and difficult to feed. The intensive strategy is used as soon as larvae are considered mature enough to be reared easily using classical methods. The technology was used for the rearing of two species, with potential for aquaculture, the sharpsnout seabream (Diplodus puntazzo) and the white seabream (Diplodus sargus sargus). Three groups of each species were monitored for a period of 50-70 days post hatching. Survival for both species was about 54% at the end of the trials. Sharpsnout seabream larvae reached 19.6 ± 0.9 mm total length and 107.2±31.9mg body weight 50 days post hatching. White seabream larvae 60 days post hatching reached 32.7 ± 2.7 mm total length and 450±70mg body weight. In order to verify the economical viability of the technology, the individual production cost for each species was estimated and reached €0.027 for white seabream and €0.043 for sharpsnout seabream. Results indicate the reliability of the technology for the larval rearing of the two species. © 2004 Kluwer Academic Publishers. |
Magoulas, A Mitochondrial DNA Incollection Cadrin, S; Friedland, K; Waldman, J (Ed.): Stock Ιdentification Μethods, pp. 311–330, ELSEVIER Academic Press, 2004, (Backup Publisher: ELSEVIER Academic Press Section: 16). @incollection{magoulas_mitochondrial_2004, title = {Mitochondrial DNA}, author = {A Magoulas}, editor = {S Cadrin and K Friedland and J Waldman}, year = {2004}, date = {2004-01-01}, booktitle = {Stock Ιdentification Μethods}, pages = {311--330}, publisher = {ELSEVIER Academic Press}, note = {Backup Publisher: ELSEVIER Academic Press Section: 16}, keywords = {}, pubstate = {published}, tppubtype = {incollection} } |
Katharios, P; Iliopoulou-Georgudaki, J; Antimisiaris, S; Verri, T; Toma, P; Acierno, R; Maffia, M Pharmacokinetics of cephalexin in sea bream, Sparus aurata (L.), after a single intraperitoneal injection Journal Article Journal of Applied Ichthyology, 20 (5), pp. 422–426, 2004, ISSN: 01758659. @article{katharios_pharmacokinetics_2004, title = {Pharmacokinetics of cephalexin in sea bream, Sparus aurata (L.), after a single intraperitoneal injection}, author = {P Katharios and J Iliopoulou-Georgudaki and S Antimisiaris and T Verri and P Toma and R Acierno and M Maffia}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-5444259750&doi=10.1111%2fj.1439-0426.2004.00578.x&partnerID=40&md5=7ca32ad32162890875c6aca6aff4f216}, doi = {10.1111/j.1439-0426.2004.00578.x}, issn = {01758659}, year = {2004}, date = {2004-01-01}, journal = {Journal of Applied Ichthyology}, volume = {20}, number = {5}, pages = {422--426}, abstract = {The pharmacokinetic properties of cephalexin were studied in sea bream (mean weight 77 g), Sparus aurata (L.) after a single intraperitoneal injection (200 mg kg-1). Pharmacokinetic analysis of the serum concentrations vs time points obtained was performed using non-compartmental analysis and a compartmental pharmacokinetic model approach. In the latter case, a two-compartment open model with a lag time gave the best fitting. The maximum peak serum concentration was 5.018 mg ml-1 kg-1 1 h post-treatment. The area under the curve (AUC) cephalexin was 17.394 mg·h kg-1 and the elimination half-life of 1.83 h. © 2004 Blackwell Verlag, Berlin.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The pharmacokinetic properties of cephalexin were studied in sea bream (mean weight 77 g), Sparus aurata (L.) after a single intraperitoneal injection (200 mg kg-1). Pharmacokinetic analysis of the serum concentrations vs time points obtained was performed using non-compartmental analysis and a compartmental pharmacokinetic model approach. In the latter case, a two-compartment open model with a lag time gave the best fitting. The maximum peak serum concentration was 5.018 mg ml-1 kg-1 1 h post-treatment. The area under the curve (AUC) cephalexin was 17.394 mg·h kg-1 and the elimination half-life of 1.83 h. © 2004 Blackwell Verlag, Berlin. |
Rigos, G; Tyrpenou, ; Nengas, I; Alexis, A E M; Poulou, Athanasso F; Troisi, G M Poor bioavailability of oxytetracycline in sharpsnout sea bream Diplodus puntazzo Journal Article Aquaculture, 235 , pp. 489 – 497, 2004. @article{rigos_poor_2004, title = {Poor bioavailability of oxytetracycline in sharpsnout sea bream Diplodus puntazzo}, author = {G Rigos and Tyrpenou and I Nengas and A E M Alexis and F Athanasso Poulou and G M Troisi}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-1542280919&partnerID=40&md5=7c1f6e8e157f8928bcb1086f90bea99a}, year = {2004}, date = {2004-01-01}, journal = {Aquaculture}, volume = {235}, pages = {489 -- 497}, abstract = {The pharmacokinetics of oxytetracycline (OTC) were investigated following single intravascular injection (40 mg/kg) in sharpsnout sea bream (90 g) at 19°C. The distribution half-life (t1/2α) and the elimination half-life (t1/2β) of OTC were calculated to be 1.4 and 35 h, respectively. The apparent volume of distribution of the drug at steady-state (Vd(ss)) was found to be 4 l/kg. The total clearance rate (CLT) of the drug was low (0.08 l/kg h). Repeated attempts to investigate the bioavailability of OTC following oral administration (75 mg/kg; forced-fed or via the feed), revealed undetectable levels of the drug in plasma and muscle samples. Poor tissue absorption is likely due to significant hepatic metabolism of OTC in sharpsnout sea bream, but this requires further investigation. Thus, oral administration of OTC in farmed sharpsnout sea bream should be discouraged in favour of other routes of administration or other antibiotic drugs. © 2003 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The pharmacokinetics of oxytetracycline (OTC) were investigated following single intravascular injection (40 mg/kg) in sharpsnout sea bream (90 g) at 19°C. The distribution half-life (t1/2α) and the elimination half-life (t1/2β) of OTC were calculated to be 1.4 and 35 h, respectively. The apparent volume of distribution of the drug at steady-state (Vd(ss)) was found to be 4 l/kg. The total clearance rate (CLT) of the drug was low (0.08 l/kg h). Repeated attempts to investigate the bioavailability of OTC following oral administration (75 mg/kg; forced-fed or via the feed), revealed undetectable levels of the drug in plasma and muscle samples. Poor tissue absorption is likely due to significant hepatic metabolism of OTC in sharpsnout sea bream, but this requires further investigation. Thus, oral administration of OTC in farmed sharpsnout sea bream should be discouraged in favour of other routes of administration or other antibiotic drugs. © 2003 Elsevier B.V. All rights reserved. |
Rigos, G; Nengas, I; Alexis, M; Troisi, G M Potential drug (oxytetracycline and oxolinic acid) pollution from Mediterranean sparid fish farms Journal Article Aquatic Toxicology, 69 , pp. 281 – 288, 2004. @article{rigos_potential_2004, title = {Potential drug (oxytetracycline and oxolinic acid) pollution from Mediterranean sparid fish farms}, author = {G Rigos and I Nengas and M Alexis and G M Troisi}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-3242806075&partnerID=40&md5=bca8f73fe1a7ba2abb13d5ad230e6027}, year = {2004}, date = {2004-01-01}, journal = {Aquatic Toxicology}, volume = {69}, pages = {281 -- 288}, abstract = {The potential for input of two common antibacterial agents in Mediterranean fish farms, oxytetracycline (OTC) and oxolinic acid (OA), was estimated from measurements of these drugs in the faecal excretions of two important farmed sparids, gilthead sea bream, Sparus aurata and sharpsnout sea bream Diplodus puntazzo. Oxolinic acid was found to be well absorbed by gilthead sea bream (92%) and sharpsnout sea bream (88%) while the absorption of OTC was found to be considerably lower in both species (27 and 40%, respectively). These data were integrated with production records for sparids, drug dosage regimes and treatment frequency information to calculate potential annual drug release to the aquatic environment from Greek fish farms. These calculations suggest potentially significant quantities of unmetabolised OTC can be passed unabsorbed through the body of treated sparids and excreted via the faeces into the local marine environment. The situation with OA was much less pronounced. It was estimated that potentially more than 1900 kg of OTC and more than 50 kg of OA may be released via faecal excretion into the environment by sparid farms per year. Further drug may also be released via uneaten medicated feed, leached drugs and other routes of fish elimination (renal excretion, branchial secretions). Drug pollution of the marine environment in the vicinity of fish farms can have adverse ecological effects, including development of resistant bacterial populations and exposure with potential drug accumulation in aquatic fauna and flora. © 2004 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The potential for input of two common antibacterial agents in Mediterranean fish farms, oxytetracycline (OTC) and oxolinic acid (OA), was estimated from measurements of these drugs in the faecal excretions of two important farmed sparids, gilthead sea bream, Sparus aurata and sharpsnout sea bream Diplodus puntazzo. Oxolinic acid was found to be well absorbed by gilthead sea bream (92%) and sharpsnout sea bream (88%) while the absorption of OTC was found to be considerably lower in both species (27 and 40%, respectively). These data were integrated with production records for sparids, drug dosage regimes and treatment frequency information to calculate potential annual drug release to the aquatic environment from Greek fish farms. These calculations suggest potentially significant quantities of unmetabolised OTC can be passed unabsorbed through the body of treated sparids and excreted via the faeces into the local marine environment. The situation with OA was much less pronounced. It was estimated that potentially more than 1900 kg of OTC and more than 50 kg of OA may be released via faecal excretion into the environment by sparid farms per year. Further drug may also be released via uneaten medicated feed, leached drugs and other routes of fish elimination (renal excretion, branchial secretions). Drug pollution of the marine environment in the vicinity of fish farms can have adverse ecological effects, including development of resistant bacterial populations and exposure with potential drug accumulation in aquatic fauna and flora. © 2004 Elsevier B.V. All rights reserved. |
Mandalakis, M; Gustafsson, Ö; Reddy, C M; Xu, L Radiocarbon apportionment of fossil versus biofuel combustion sources of polycyclic aromatic hydrocarbons in the Stockholm metropolitan area Journal Article Environmental Science and Technology, 38 (20), pp. 5344–5349, 2004, ISSN: 0013936X. @article{mandalakis_radiocarbon_2004, title = {Radiocarbon apportionment of fossil versus biofuel combustion sources of polycyclic aromatic hydrocarbons in the Stockholm metropolitan area}, author = {M Mandalakis and Ö Gustafsson and C M Reddy and L Xu}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-6044256299&doi=10.1021%2fes049088x&partnerID=40&md5=dada99d9431117db76b594464cebbbb1}, doi = {10.1021/es049088x}, issn = {0013936X}, year = {2004}, date = {2004-01-01}, journal = {Environmental Science and Technology}, volume = {38}, number = {20}, pages = {5344--5349}, abstract = {Source-diagnostic markers and the isotopic composition of polycyclic aromatic hydrocarbons (PAHs) were examined in surface sediments from the greater Stockholm waterways to deduce the contribution from biomass sources to the environmental PAH load. The summed concentration of 20 PAHs ranged from 0.8 to 45.1 μg/g (dry weight) and exhibited a steep decline with increasing distance from the city center evidencing that sources within the metropolitan area of Stockholm dominate its PAH burden. Several diagnostic PAH ratios indicated an overwhelming predominance of pyrogenic sources over the petrogenic ones, while retene and 1,7-dimethylphenanthrene were unable to correctly evaluate the contribution from biomass combustion. The stable carbon isotope composition (δ13C) of individual PAHs ranged from -24.8 to -27.0% but also was proved inefficient to discriminate between different types of fuels due to the overlapping signals in various sources. The Δ14C values of PAHs ranged between -550.4 and -934.1%, indicating a clear predominance of fossil fuel sources. By using an isotopic mass balance approach, we estimated that on average 17 ± 9% of PAHs derived from biomass combustion. This radiocarbon apportionment, in conjunction with detailed energy statistics for the Stockholm region, revealed that the ambient PAH burden is roughly similar, per unit energy produced, from fossil fuels and biofuels. Societies' shifting energy policies toward a larger reliance on biofuels may thus not lead to further deterioration of air quality and respiratory ailments for the urban population.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Source-diagnostic markers and the isotopic composition of polycyclic aromatic hydrocarbons (PAHs) were examined in surface sediments from the greater Stockholm waterways to deduce the contribution from biomass sources to the environmental PAH load. The summed concentration of 20 PAHs ranged from 0.8 to 45.1 μg/g (dry weight) and exhibited a steep decline with increasing distance from the city center evidencing that sources within the metropolitan area of Stockholm dominate its PAH burden. Several diagnostic PAH ratios indicated an overwhelming predominance of pyrogenic sources over the petrogenic ones, while retene and 1,7-dimethylphenanthrene were unable to correctly evaluate the contribution from biomass combustion. The stable carbon isotope composition (δ13C) of individual PAHs ranged from -24.8 to -27.0% but also was proved inefficient to discriminate between different types of fuels due to the overlapping signals in various sources. The Δ14C values of PAHs ranged between -550.4 and -934.1%, indicating a clear predominance of fossil fuel sources. By using an isotopic mass balance approach, we estimated that on average 17 ± 9% of PAHs derived from biomass combustion. This radiocarbon apportionment, in conjunction with detailed energy statistics for the Stockholm region, revealed that the ambient PAH burden is roughly similar, per unit energy produced, from fossil fuels and biofuels. Societies' shifting energy policies toward a larger reliance on biofuels may thus not lead to further deterioration of air quality and respiratory ailments for the urban population. |
Mylonas, C C; Kyriakou, Y; Sigelaki, I; Georgiou, G; Stephanou, D; Divanach, P Reproductive biology of the shi drum (Umrina cirrosa) in captivity and induction of spawning using GNRHA Journal Article Israeli Journal of Aquaculture - Bamidgeh, 56 (2), pp. 77–94, 2004, ISSN: 0792156X. @article{mylonas_reproductive_2004, title = {Reproductive biology of the shi drum (Umrina cirrosa) in captivity and induction of spawning using GNRHA}, author = {C C Mylonas and Y Kyriakou and I Sigelaki and G Georgiou and D Stephanou and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-3142760090&partnerID=40&md5=9f2ed2f2e75086d9b738a1ab4dedd38d}, issn = {0792156X}, year = {2004}, date = {2004-01-01}, journal = {Israeli Journal of Aquaculture - Bamidgeh}, volume = {56}, number = {2}, pages = {77--94}, abstract = {The reproductive biology of the shi drum (Umbrina cirrosa) in culture was histologically examined and sperm quality was monitored during an entire reproductive period. Already in April, the ovary contained oocytes in all stages of maturation, from primary oocytes to full vitellogenesis, as expected from a group-synchronous multiple-batch spawning fish. Vitellogenesis of the first batch of oocytes occurred very rapidly and their mean diameter (500 μm) did not increase significantly (p>0.05) as the reproductive period proceeded. The spermiation index peaked in May-June, but fish never produced copious amounts of milt upon abdominal pressure. The spermatozoa motility percentage remained unchanged throughout the spawning season (80%) and a significant percentage (40%) maintained viability after overnight storage at 4°C. Sperm density and motility duration increased during the reproductive period and varied 13-26 × 109 spermatozoa/ml and 26-40 s, respectively. Spontaneous spawning was not observed during the two-year study. Injection of post-vitellogenic females with an agonist of gonadotropin-releasing hormone (GnRHa) was successful in inducing a single spawning after two days, with fertilization, hatching and 4-day larval survival rates of 65%, 42-76% and 46-80%, respectively. The results underline the failure of female shi drum in culture to undergo final oocyte maturation and, although GnRHa injection was effective in inducing spawning of viable eggs, multiple treatments did not induce multiple spawns, as was expected from fish with multiple-batch group-synchronous ovarian biology.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The reproductive biology of the shi drum (Umbrina cirrosa) in culture was histologically examined and sperm quality was monitored during an entire reproductive period. Already in April, the ovary contained oocytes in all stages of maturation, from primary oocytes to full vitellogenesis, as expected from a group-synchronous multiple-batch spawning fish. Vitellogenesis of the first batch of oocytes occurred very rapidly and their mean diameter (500 μm) did not increase significantly (p>0.05) as the reproductive period proceeded. The spermiation index peaked in May-June, but fish never produced copious amounts of milt upon abdominal pressure. The spermatozoa motility percentage remained unchanged throughout the spawning season (80%) and a significant percentage (40%) maintained viability after overnight storage at 4°C. Sperm density and motility duration increased during the reproductive period and varied 13-26 × 109 spermatozoa/ml and 26-40 s, respectively. Spontaneous spawning was not observed during the two-year study. Injection of post-vitellogenic females with an agonist of gonadotropin-releasing hormone (GnRHa) was successful in inducing a single spawning after two days, with fertilization, hatching and 4-day larval survival rates of 65%, 42-76% and 46-80%, respectively. The results underline the failure of female shi drum in culture to undergo final oocyte maturation and, although GnRHa injection was effective in inducing spawning of viable eggs, multiple treatments did not induce multiple spawns, as was expected from fish with multiple-batch group-synchronous ovarian biology. |
Rigos, G; Tyrpenou, A E; Nengas, I; Alexis, M; Troisi, G M The kinetic profile of oxolinic acid in sharpsnout sea bream, Diplodus puntazzo (Cetti 1777) Journal Article Aquaculture Research, 35 , pp. 1299 – 1304, 2004. @article{rigos_kinetic_2004, title = {The kinetic profile of oxolinic acid in sharpsnout sea bream, Diplodus puntazzo (Cetti 1777)}, author = {G Rigos and A E Tyrpenou and I Nengas and M Alexis and G M Troisi}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-9344262875&partnerID=40&md5=747d42b180b9ebf50171888f16b799b3}, year = {2004}, date = {2004-01-01}, journal = {Aquaculture Research}, volume = {35}, pages = {1299 -- 1304}, abstract = {The pharmacokinetics of oxolinic acid (OA) were investigated after a single intra-vascular injection (20 mg kg-1 fish) in sharpsnout sea bream (90 g), a promising new euryhaline species for Mediterranean fish farming. The distribution half-life (t1/2α) and the elimination half-life (t1/2β) of OA were calculated to be 0.4 and 10 h respectively. The apparent volume of distribution at steady-state (Vd(ss)) and total clearance rate (CLT) of the drug were found to be 2.1 L kg and 0.2 L kg-1 h-1 respectively. The bioavailability (F%) of OA following oral administration (40 mg kg-1 fish) fish) was estimated to be 15%. The results indicate a rapid distribution and elimination of the drug, moderate tissue penetration, but low absorption in sharpsnout sea bream. The kinetic profile of OA found in this species is comparable with that observed in another well-known sparid, gilthead sea bream.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The pharmacokinetics of oxolinic acid (OA) were investigated after a single intra-vascular injection (20 mg kg-1 fish) in sharpsnout sea bream (90 g), a promising new euryhaline species for Mediterranean fish farming. The distribution half-life (t1/2α) and the elimination half-life (t1/2β) of OA were calculated to be 0.4 and 10 h respectively. The apparent volume of distribution at steady-state (Vd(ss)) and total clearance rate (CLT) of the drug were found to be 2.1 L kg and 0.2 L kg-1 h-1 respectively. The bioavailability (F%) of OA following oral administration (40 mg kg-1 fish) fish) was estimated to be 15%. The results indicate a rapid distribution and elimination of the drug, moderate tissue penetration, but low absorption in sharpsnout sea bream. The kinetic profile of OA found in this species is comparable with that observed in another well-known sparid, gilthead sea bream. |
Mandalakis, M; Stephanou, E G Wet deposition of polychlorinated biphenyls in the eastern Mediterranean Journal Article Environmental Science and Technology, 38 (11), pp. 3011–3018, 2004, ISSN: 0013936X. @article{mandalakis_wet_2004, title = {Wet deposition of polychlorinated biphenyls in the eastern Mediterranean}, author = {M Mandalakis and E G Stephanou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-2542447209&doi=10.1021%2fes030078q&partnerID=40&md5=62f74bf720be4dfe07eb56dbbd3b4ed2}, doi = {10.1021/es030078q}, issn = {0013936X}, year = {2004}, date = {2004-01-01}, journal = {Environmental Science and Technology}, volume = {38}, number = {11}, pages = {3011--3018}, abstract = {The concentrations of polychlorinated biphenyls (PCBs) were measured in rain samples collected from a semi-urban and a marine background site of the eastern Mediterranean. The concentration of ΣPCB (sum of 54 PCB congeners) in the city of Heraklion (2.9 ± 1.9 ng L-1) was not significantly higher than the corresponding concentration measured at the background sampling station of Finokalia (1.9 ± 0.9 ng L-1). In both sites, the sum of tri- and tetrachlorinated congeners accounted for more than 55% of ΣPCB in rainwater. For all samples, the percentage of particle-bound PCBs ranged between 6.6% and 63.8%, providing an average value of 31 ± 18%. The washout ratios of particulate PCBs (Wp) were constant for individual congeners regardless the degree of chlorination. Average W P values ranged between 1.9 × 105 and 5.2 × 105 while a value of 2.7(±1.3) × 105 was deduced for ΣPCB. The corresponding washout ratios for gaseous PCBs were substantially lower and ranged between 7 × 103 (PCB 99) and 1. 3 × 105 (PCB 180). Washout ratios of gaseous PCBs were also calculated based on Henry's law, and they were found to be 30-920 times lower than those obtained from field measurements. On the basis of our data, the wet deposition flux of ΣPCB in the eastern Mediterranean should approach 820 ng m-2 yr-1. This flux is similar with the values recently reported for several background sites of the United States and Europe, but it is 1 order of magnitude lower than the flux of PCBs measured in the western Mediterranean 16 yr ago.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The concentrations of polychlorinated biphenyls (PCBs) were measured in rain samples collected from a semi-urban and a marine background site of the eastern Mediterranean. The concentration of ΣPCB (sum of 54 PCB congeners) in the city of Heraklion (2.9 ± 1.9 ng L-1) was not significantly higher than the corresponding concentration measured at the background sampling station of Finokalia (1.9 ± 0.9 ng L-1). In both sites, the sum of tri- and tetrachlorinated congeners accounted for more than 55% of ΣPCB in rainwater. For all samples, the percentage of particle-bound PCBs ranged between 6.6% and 63.8%, providing an average value of 31 ± 18%. The washout ratios of particulate PCBs (Wp) were constant for individual congeners regardless the degree of chlorination. Average W P values ranged between 1.9 × 105 and 5.2 × 105 while a value of 2.7(±1.3) × 105 was deduced for ΣPCB. The corresponding washout ratios for gaseous PCBs were substantially lower and ranged between 7 × 103 (PCB 99) and 1. 3 × 105 (PCB 180). Washout ratios of gaseous PCBs were also calculated based on Henry's law, and they were found to be 30-920 times lower than those obtained from field measurements. On the basis of our data, the wet deposition flux of ΣPCB in the eastern Mediterranean should approach 820 ng m-2 yr-1. This flux is similar with the values recently reported for several background sites of the United States and Europe, but it is 1 order of magnitude lower than the flux of PCBs measured in the western Mediterranean 16 yr ago. |
2003 |
Bargelloni, L; Alarcon, J A; Alvarez, M C; Penzo, E; Magoulas, A; Reis, C; Patarnello, T Discord in the family Sparidae (Teleostei): divergent phylogeographical patterns across the Atlantic-Mediterranean divide Journal Article Journal of Evolutionary Biology, 16 (6), pp. 1149–1158, 2003, ISSN: 1010-061X, 1420-9101. @article{bargelloni_discord_2003, title = {Discord in the family Sparidae (Teleostei): divergent phylogeographical patterns across the Atlantic-Mediterranean divide}, author = {L Bargelloni and J A Alarcon and M C Alvarez and E Penzo and A Magoulas and C Reis and T Patarnello}, url = {http://doi.wiley.com/10.1046/j.1420-9101.2003.00620.x}, doi = {10.1046/j.1420-9101.2003.00620.x}, issn = {1010-061X, 1420-9101}, year = {2003}, date = {2003-11-01}, urldate = {2020-08-09}, journal = {Journal of Evolutionary Biology}, volume = {16}, number = {6}, pages = {1149--1158}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Martyniuk, C J; Perry, G M L; Mogahadam, H K; Ferguson, M M; Danzmann, R G The genetic architecture of correlations among growth-related traits and male age at maturation in rainbow trout Journal Article Journal of Fish Biology, 63 (3), pp. 746–764, 2003, ISSN: 0022-1112, 1095-8649. @article{martyniuk_genetic_2003, title = {The genetic architecture of correlations among growth-related traits and male age at maturation in rainbow trout}, author = {C J Martyniuk and G M L Perry and H K Mogahadam and M M Ferguson and R G Danzmann}, url = {http://doi.wiley.com/10.1046/j.1095-8649.2003.00188.x}, doi = {10.1046/j.1095-8649.2003.00188.x}, issn = {0022-1112, 1095-8649}, year = {2003}, date = {2003-09-01}, urldate = {2020-08-11}, journal = {Journal of Fish Biology}, volume = {63}, number = {3}, pages = {746--764}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Manios, Andreas; Tosca, Androniki; Volakakis, Evaggelos; Leivadara, Moshoula; Tsiftsis, Dimitris Computer assisted evaluation of wound healing in chronic ulcers Journal Article Computers in Biology and Medicine, 33 (4), pp. 311–317, 2003, ISSN: 00104825. @article{manios_computer_2003, title = {Computer assisted evaluation of wound healing in chronic ulcers}, author = {Andreas Manios and Androniki Tosca and Evaggelos Volakakis and Moshoula Leivadara and Dimitris Tsiftsis}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0010482502000823}, doi = {10.1016/S0010-4825(02)00082-3}, issn = {00104825}, year = {2003}, date = {2003-07-01}, urldate = {2020-08-17}, journal = {Computers in Biology and Medicine}, volume = {33}, number = {4}, pages = {311--317}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Venou, B; Alexis, M N; Fountoulaki, E; Nengas, I; Apostolopoulou, M; Castritsi-Cathariou, I Aquaculture, 225 (1-4), pp. 207–223, 2003, ISSN: 00448486. @article{venou_effect_2003, title = {Effect of extrusion of wheat and corn on gilthead sea bream (Sparus aurata) growth, nutrient utilization efficiency, rates of gastric evacuation and digestive enzyme activities}, author = {B Venou and M N Alexis and E Fountoulaki and I Nengas and M Apostolopoulou and I Castritsi-Cathariou}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848603002904}, doi = {10.1016/S0044-8486(03)00290-4}, issn = {00448486}, year = {2003}, date = {2003-07-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {225}, number = {1-4}, pages = {207--223}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Fountoulaki, E; Alexis, M N; Nengas, I; Venou, B Aquaculture, 225 (1-4), pp. 309–323, 2003, ISSN: 00448486. @article{fountoulaki_effects_2003, title = {Effects of dietary arachidonic acid (20:4n-6), on growth, body composition, and tissue fatty acid profile of gilthead bream fingerlings (Sparus aurata L.)}, author = {E Fountoulaki and M N Alexis and I Nengas and B Venou}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848603002989}, doi = {10.1016/S0044-8486(03)00298-9}, issn = {00448486}, year = {2003}, date = {2003-07-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {225}, number = {1-4}, pages = {309--323}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Grigorakis, K; Taylor, K D A; Alexis, M N Aquaculture, 225 (1-4), pp. 109–119, 2003, ISSN: 00448486. @article{grigorakis_organoleptic_2003, title = {Organoleptic and volatile aroma compounds comparison of wild and cultured gilthead sea bream (Sparus aurata): sensory differences and possible chemical basis}, author = {K Grigorakis and K D A Taylor and M N Alexis}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848603002837}, doi = {10.1016/S0044-8486(03)00283-7}, issn = {00448486}, year = {2003}, date = {2003-07-01}, urldate = {2020-08-10}, journal = {Aquaculture}, volume = {225}, number = {1-4}, pages = {109--119}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Probst, H C; Lagnel, J; Kollias, G; van den Broek, M Inducible transgenic mice reveal resting dendritic cells as potent inducers of CD8(+) Ŧ cell tolerance Journal Article Immunity, 18 (5), pp. 713–720, 2003, (ISBN: 1074-7613). @article{probst_inducible_2003, title = {Inducible transgenic mice reveal resting dendritic cells as potent inducers of CD8(+) Ŧ cell tolerance}, author = {H C Probst and J Lagnel and G Kollias and M van den Broek}, url = {<Go to ISI>://000182962200015}, year = {2003}, date = {2003-05-01}, journal = {Immunity}, volume = {18}, number = {5}, pages = {713--720}, abstract = {Dendritic cells (DC) are inducers of immune responses par excellence. They also seem responsible for the induction of peripheral T cell tolerance. To investigate these opposite functions of DC, we generated a Cre/LoxP-based system that allows inducible antigen presentation by DC in vivo. This enables us to study the immunogical consequences of antigen presentation by resting versus mature DC without adoptively transferring DC and with physiological numbers of endogenous, naive responder T cells. We found that presentation of LCMV-derived CTL epitopes by resting DC resulted in antigen-specific tolerance, which could not be broken by subsequent infection with LCMV. On the other hand, antigen presentation by activated DC primed endogenous CTL to expand and to develop protective effector function.}, note = {ISBN: 1074-7613}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dendritic cells (DC) are inducers of immune responses par excellence. They also seem responsible for the induction of peripheral T cell tolerance. To investigate these opposite functions of DC, we generated a Cre/LoxP-based system that allows inducible antigen presentation by DC in vivo. This enables us to study the immunogical consequences of antigen presentation by resting versus mature DC without adoptively transferring DC and with physiological numbers of endogenous, naive responder T cells. We found that presentation of LCMV-derived CTL epitopes by resting DC resulted in antigen-specific tolerance, which could not be broken by subsequent infection with LCMV. On the other hand, antigen presentation by activated DC primed endogenous CTL to expand and to develop protective effector function. |
Grigorakis, K; Taylor, K D A; Alexis, M N Seasonal patterns of spoilage of ice-stored cultured gilthead sea bream (Sparus aurata) Journal Article Food Chemistry, 81 (2), pp. 263–268, 2003, ISSN: 03088146. @article{grigorakis_seasonal_2003, title = {Seasonal patterns of spoilage of ice-stored cultured gilthead sea bream (Sparus aurata)}, author = {K Grigorakis and K D A Taylor and M N Alexis}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0308814602004211}, doi = {10.1016/S0308-8146(02)00421-1}, issn = {03088146}, year = {2003}, date = {2003-05-01}, urldate = {2020-08-10}, journal = {Food Chemistry}, volume = {81}, number = {2}, pages = {263--268}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Tyrpenou, A E; Kotzamanis, Y P; Alexis, M N Aquaculture, 220 (1-4), pp. 633–642, 2003, ISSN: 00448486. @article{tyrpenou_flumequine_2003, title = {Flumequine depletion from muscle plus skin tissue of gilthead seabream (Sparus aurata L.) fed flumequine medicated feed in seawater at 18 and 24 °C}, author = {A E Tyrpenou and Y P Kotzamanis and M N Alexis}, url = {https://linkinghub.elsevier.com/retrieve/pii/S004484860200251X}, doi = {10.1016/S0044-8486(02)00251-X}, issn = {00448486}, year = {2003}, date = {2003-04-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {220}, number = {1-4}, pages = {633--642}, abstract = {We examined flumequine depletion from muscle plus skin of gilthead seabream held in seawater at 18 and 24°C. Seven groups of 10 fish each were sampled at intervals ranging from 24 to 168 h after in-feed administration of flumequine at 35 mg/kg/day for 5 days. Muscle plus skin tissue samples were analyzed for flumequine by high-performance liquid chromatography and fluorescence detection (HPLC-SFD). Parent flumequine concentrations declined rapidly from muscle plus skin after dosing with elimination half-lives of t1/2=22.14 and 21.43 h at 18 and 24°C, respectively. Withdrawal periods for the maximum residue limit (MRL) of 600 μg/kg flumequine in muscle plus skin at 95% tolerance limit were 106.08 and 75.84 h at 18 and 24°C, respectively, after treatment. © 2003 Elsevier Science B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We examined flumequine depletion from muscle plus skin of gilthead seabream held in seawater at 18 and 24°C. Seven groups of 10 fish each were sampled at intervals ranging from 24 to 168 h after in-feed administration of flumequine at 35 mg/kg/day for 5 days. Muscle plus skin tissue samples were analyzed for flumequine by high-performance liquid chromatography and fluorescence detection (HPLC-SFD). Parent flumequine concentrations declined rapidly from muscle plus skin after dosing with elimination half-lives of t1/2=22.14 and 21.43 h at 18 and 24°C, respectively. Withdrawal periods for the maximum residue limit (MRL) of 600 μg/kg flumequine in muscle plus skin at 95% tolerance limit were 106.08 and 75.84 h at 18 and 24°C, respectively, after treatment. © 2003 Elsevier Science B.V. All rights reserved. |
Sfakianakis, D G; Koumoundouros, G; Anezaki, L; Divanach, P; Kentouri, M Development of a saddleback-like syndrome in reared white seabream Diplodus sargus (Linnaeus, 1758) Journal Article Aquaculture, 217 (1-4), pp. 673–676, 2003, ISSN: 00448486. @article{sfakianakis_development_2003, title = {Development of a saddleback-like syndrome in reared white seabream Diplodus sargus (Linnaeus, 1758)}, author = {D G Sfakianakis and G Koumoundouros and L Anezaki and P Divanach and M Kentouri}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848602001990}, doi = {10.1016/S0044-8486(02)00199-0}, issn = {00448486}, year = {2003}, date = {2003-03-01}, urldate = {2020-08-17}, journal = {Aquaculture}, volume = {217}, number = {1-4}, pages = {673--676}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Koulouri, P T; Dounas, C G; Eleftheriou, A S A new apparatus for the direct measurement of otter trawling effects on the epibenthic and hyperbenthic macrofauna Journal Article Journal of the Marine Biological Association of the United Kingdom, 83 (6), pp. 1363–1368, 2003, ISSN: 00253154. @article{koulouri_new_2003, title = {A new apparatus for the direct measurement of otter trawling effects on the epibenthic and hyperbenthic macrofauna}, author = {P T Koulouri and C G Dounas and A S Eleftheriou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-1042267134&doi=10.1017%2fS0025315403008828&partnerID=40&md5=1086ff028266b8fe562a6160f8f82091}, doi = {10.1017/S0025315403008828}, issn = {00253154}, year = {2003}, date = {2003-01-01}, journal = {Journal of the Marine Biological Association of the United Kingdom}, volume = {83}, number = {6}, pages = {1363--1368}, abstract = {A towed trawl simulator sledge (TTSS2) for collecting quantitatively small macrobenthic animals disturbed by the passage of otter trawl groundrope typical of the local fishery is described and illustrated. The TTSS2 was towed from a surface vessel at a speed within the range of commercial trawls, incorporating three sampling nets that open and close by means of an electro-mechanical system. An odometer in contact with the seabed provided a continuous record of the trawled ground. The degree of efficiency of the TTSS2 - by means of the attached groundrope that stirs up the surface of the bottom ahead of the nets -was tested in the northern continental shelf of Crete (eastern Mediterranean). Additional trials with a much heavier groundropc were also performed. Analysis and comparison of TTSS2 samples with endobenthic, hyperbenthic and planktonic samples collected by means of conventional sampling gears indicate the presence of a well-defined and distinct fauna in the plume of resuspended sediment behind the groundrope.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A towed trawl simulator sledge (TTSS2) for collecting quantitatively small macrobenthic animals disturbed by the passage of otter trawl groundrope typical of the local fishery is described and illustrated. The TTSS2 was towed from a surface vessel at a speed within the range of commercial trawls, incorporating three sampling nets that open and close by means of an electro-mechanical system. An odometer in contact with the seabed provided a continuous record of the trawled ground. The degree of efficiency of the TTSS2 - by means of the attached groundrope that stirs up the surface of the bottom ahead of the nets -was tested in the northern continental shelf of Crete (eastern Mediterranean). Additional trials with a much heavier groundropc were also performed. Analysis and comparison of TTSS2 samples with endobenthic, hyperbenthic and planktonic samples collected by means of conventional sampling gears indicate the presence of a well-defined and distinct fauna in the plume of resuspended sediment behind the groundrope. |
Koutandos, E A numerical and experimental study on the hydrodynamics of a heave motion floating breakwater Inproceedings 2003, (Publication Title: XXX IAHR Congress). @inproceedings{koutandos_numerical_2003, title = {A numerical and experimental study on the hydrodynamics of a heave motion floating breakwater}, author = {E Koutandos}, year = {2003}, date = {2003-01-01}, volume = {JFK competition}, note = {Publication Title: XXX IAHR Congress}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Plaiti, W; Tselepides, A Amphipod community structure in an area of intensive natural and antropogenic disturbance (Termaicos Bay in the Mediteranean Sea). Inproceedings Tunis, Tunisia, 2003, (Publication Title: The11th International Colloquium on Amphipoda, Type: Abstract). @inproceedings{plaiti_amphipod_2003, title = {Amphipod community structure in an area of intensive natural and antropogenic disturbance (Termaicos Bay in the Mediteranean Sea).}, author = {W Plaiti and A Tselepides}, year = {2003}, date = {2003-01-01}, address = {Tunis, Tunisia}, note = {Publication Title: The11th International Colloquium on Amphipoda, Type: Abstract}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Iliopoulou-Georgudaki, J; Kantzaris, V; Katharios, P; Kaspiris, P; Georgiadis, Th.; Montesantou, B An application of different bioindicators for assessing water quality: A case study in the rivers Alfeios and Pineios (Peloponnisos, Greece) Journal Article Ecological Indicators, 2 (4), pp. 345–360, 2003, ISSN: 1470160X, (Publisher: Elsevier). @article{iliopoulou-georgudaki_application_2003, title = {An application of different bioindicators for assessing water quality: A case study in the rivers Alfeios and Pineios (Peloponnisos, Greece)}, author = {J Iliopoulou-Georgudaki and V Kantzaris and P Katharios and P Kaspiris and Th. Georgiadis and B Montesantou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0141993249&doi=10.1016%2fS1470-160X%2803%2900004-9&partnerID=40&md5=0655df035f5bd0c4f216644b1fe3c623}, doi = {10.1016/S1470-160X(03)00004-9}, issn = {1470160X}, year = {2003}, date = {2003-01-01}, journal = {Ecological Indicators}, volume = {2}, number = {4}, pages = {345--360}, abstract = {A number of bioindicators and biotic indices and scores based on benthic macroinvertebrates, diatoms, fishes, aquatic and riparian vegetation in relation to physicochemical parameters have been applied in assessing the water quality of the rivers Alfeios and Pineios (Peloponnisos, Greece). According to the findings, the water quality in both rivers varied from very poor to very good. Among the bioindicators used, the benthic macroinvertebrates seem to be the most reliable. The BBI and IBE were the most applicable indexes while the applicability of the IBMWP and IASPT in the Greek region can be enforced with the inclusion of the Diptreran family Rhagionidae and the Coleopteran family Elminthidae. © 2003 Published by Elsevier Science Ltd.}, note = {Publisher: Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {article} } A number of bioindicators and biotic indices and scores based on benthic macroinvertebrates, diatoms, fishes, aquatic and riparian vegetation in relation to physicochemical parameters have been applied in assessing the water quality of the rivers Alfeios and Pineios (Peloponnisos, Greece). According to the findings, the water quality in both rivers varied from very poor to very good. Among the bioindicators used, the benthic macroinvertebrates seem to be the most reliable. The BBI and IBE were the most applicable indexes while the applicability of the IBMWP and IASPT in the Greek region can be enforced with the inclusion of the Diptreran family Rhagionidae and the Coleopteran family Elminthidae. © 2003 Published by Elsevier Science Ltd. |
Karambas, T; Koutandos, E; Karathanasi, E Boussinesq modeling of the alongshore sediment transport Inproceedings 2003, (Publication Title: XXX IAHR Congress). @inproceedings{karambas_boussinesq_2003, title = {Boussinesq modeling of the alongshore sediment transport}, author = {T Karambas and E Koutandos and E Karathanasi}, year = {2003}, date = {2003-01-01}, volume = {Α}, note = {Publication Title: XXX IAHR Congress}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Mandalakis, M; Berresheim, H; Stephanou, E G Direct evidence for destruction of polychlorobiphenyls by OH radicals in the subtropical troposphere Journal Article Environmental Science and Technology, 37 (3), pp. 542–547, 2003, ISSN: 0013936X. @article{mandalakis_direct_2003, title = {Direct evidence for destruction of polychlorobiphenyls by OH radicals in the subtropical troposphere}, author = {M Mandalakis and H Berresheim and E G Stephanou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0346668318&doi=10.1021%2fes020163i&partnerID=40&md5=cc89166d5dfc3f872f49f7a04a15e1bf}, doi = {10.1021/es020163i}, issn = {0013936X}, year = {2003}, date = {2003-01-01}, journal = {Environmental Science and Technology}, volume = {37}, number = {3}, pages = {542--547}, abstract = {Although laboratory studies have indicated that OH radicals readily oxidize polychorobiphenyls (PCBs) in the gas-phase, this mechanism has never been positively demonstrated under real atmospheric conditions. By applying elaborated sampling and analytical techniques we achieved for the first time simultaneously a field study of the diurnal atmospheric cycle of OH radical and PCBs in a remote site of eastern Mediterranean. In all cases, the concentration of ΣPCB (sum of 27 congeners) showed a characteristic depletion during daytime, while the concentration of OH radicals was at the maximum levels. By assuming that the depletion of PCBs was caused solely by the destruction from OH radicals, PCB-OH rate constants (KOH) of different PCB congeners were determined from field data by applying the relative rate method. Our field-determined KOH values were notably coherent with those previously measured in laboratory experiments. In all measurement periods, the KOH values consistently decreased in sequence for those compounds showing an increasing degree of chlorination on the biphenyl group. Bytaking into account KOH values and latitude-dependent concentration of OH radicals, it was estimated that, near to tropical and subtropical regions, the atmospheric lifetimes of PCBs 8 and 110 should be substantially low (10 and 20 days, respectively). A significant fraction of PCBs should be destroyed during their residence over tropical/subtropical regions, due to the intensive destruction by OH.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Although laboratory studies have indicated that OH radicals readily oxidize polychorobiphenyls (PCBs) in the gas-phase, this mechanism has never been positively demonstrated under real atmospheric conditions. By applying elaborated sampling and analytical techniques we achieved for the first time simultaneously a field study of the diurnal atmospheric cycle of OH radical and PCBs in a remote site of eastern Mediterranean. In all cases, the concentration of ΣPCB (sum of 27 congeners) showed a characteristic depletion during daytime, while the concentration of OH radicals was at the maximum levels. By assuming that the depletion of PCBs was caused solely by the destruction from OH radicals, PCB-OH rate constants (KOH) of different PCB congeners were determined from field data by applying the relative rate method. Our field-determined KOH values were notably coherent with those previously measured in laboratory experiments. In all measurement periods, the KOH values consistently decreased in sequence for those compounds showing an increasing degree of chlorination on the biphenyl group. Bytaking into account KOH values and latitude-dependent concentration of OH radicals, it was estimated that, near to tropical and subtropical regions, the atmospheric lifetimes of PCBs 8 and 110 should be substantially low (10 and 20 days, respectively). A significant fraction of PCBs should be destroyed during their residence over tropical/subtropical regions, due to the intensive destruction by OH. |
Kotoulas, G; Mejuto, J; Tserpes, G; Garcia-Cortes, B; Peristeraki, P; la Serna, De J M; Magoulas, A DNA microsatellite markers in service of swordfish stock-structure analysis in the Atlantic and Mediterranean Journal Article ICCAT Col. Vol. Sci. Papers, 55 (4), pp. 1632–1639, 2003. @article{kotoulas_dna_2003, title = {DNA microsatellite markers in service of swordfish stock-structure analysis in the Atlantic and Mediterranean}, author = {G Kotoulas and J Mejuto and G Tserpes and B Garcia-Cortes and P Peristeraki and J M De la Serna and A Magoulas}, year = {2003}, date = {2003-01-01}, journal = {ICCAT Col. Vol. Sci. Papers}, volume = {55}, number = {4}, pages = {1632--1639}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Polymenakou, P N; Hatziyanni, E; Tselepides, A; Stephanou, E G Effect of incubation experiments on the distribution of different lipid classes (aliphatic hydrocarbons, diglycerides, free fatty acids, glycolipids, phospholipids) in sediments of the Cretan Sea Inproceedings Hersonissos, Crete, Greece, 2003, (Publication Title: 7th Symposium of Oceanography & Fisheries Type: Poster). @inproceedings{polymenakou_effect_2003, title = {Effect of incubation experiments on the distribution of different lipid classes (aliphatic hydrocarbons, diglycerides, free fatty acids, glycolipids, phospholipids) in sediments of the Cretan Sea}, author = {P N Polymenakou and E Hatziyanni and A Tselepides and E G Stephanou}, year = {2003}, date = {2003-01-01}, address = {Hersonissos, Crete, Greece}, note = {Publication Title: 7th Symposium of Oceanography & Fisheries Type: Poster}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Tsigenopoulos, C S; Hellemans, B; Chistiakov, D A; Libertini, A; Kotoulas, G; Volckaert, F Eleven new microsatellites of the sea bass (Dicentrarchus labrax L.) Journal Article Molecular Ecology Notes, 3 (3), pp. 352–354, 2003, ISSN: 14718278. @article{tsigenopoulos_eleven_2003, title = {Eleven new microsatellites of the sea bass (Dicentrarchus labrax L.)}, author = {C S Tsigenopoulos and B Hellemans and D A Chistiakov and A Libertini and G Kotoulas and F Volckaert}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0041874880&doi=10.1046%2fj.1471-8286.2003.00447.x&partnerID=40&md5=821b63e875e1041a9f4dd3f9e973c928}, doi = {10.1046/j.1471-8286.2003.00447.x}, issn = {14718278}, year = {2003}, date = {2003-01-01}, journal = {Molecular Ecology Notes}, volume = {3}, number = {3}, pages = {352--354}, abstract = {Eleven polymorphic microsatellites were isolated from the sea bass, Dicentrarchus labrax, using a microsatellite enrichment protocol and selective hybridization with an (AC)12 probe. The loci showed different variation patterns in 21 unrelated sea bass individuals, with a mean number of alleles of 8.6 and a mean observed heterozygosity of 0.68. These microsatellite markers should be useful for population genetic analysis and biodiversity studies of sea bass.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Eleven polymorphic microsatellites were isolated from the sea bass, Dicentrarchus labrax, using a microsatellite enrichment protocol and selective hybridization with an (AC)12 probe. The loci showed different variation patterns in 21 unrelated sea bass individuals, with a mean number of alleles of 8.6 and a mean observed heterozygosity of 0.68. These microsatellite markers should be useful for population genetic analysis and biodiversity studies of sea bass. |
Publications
2004 |
Floating Breakwater Response to Waves Action Using a Boussinesq Model Coupled with a 2DV Elliptic Solver Journal Article Journal of Waterway, Port, Coastal, and Ocean Engineering, 130 (5), pp. 243–255, 2004, ISSN: 0733-950X, 1943-5460. |
Stock discrimination and temporal and spatial genetic variation of sardine (Sardina pilchardus) in northeastern Atlantic, with a combined analysis of nuclear (microsatellites and allozymes) and mitochondrial DNA markers. Inproceedings Kasapidis, P (Ed.): 2004 ICES Annual Science Conference, 2004. |
Aquaculture, 232 (1-4), pp. 407–424, 2004, ISSN: 00448486. |
Genetic comparison of wild and cultivated European populations of the gilthead sea bream (Sparus aurata) Journal Article Aquaculture, 230 (1-4), pp. 65–80, 2004, ISSN: 00448486. |
Accumulation of ivermectin in the brain of sea bream, Sparus aurata after intraperitoneal administration Journal Article Environmental Toxicology and Pharmacology, 17 (1), pp. 9–12, 2004, ISSN: 13826689. |
Analysis of the Sox gene family in the European sea bass (Dicentrarchus labrax) Journal Article Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 137 (2), pp. 279–284, 2004, ISSN: 10964959, (Publisher: Elsevier Inc.). |
Bioavailability of oxytetracycline in sea bass, Dicentrarchus labrax (L.) Journal Article Journal of Fish Diseases, 27 , pp. 119 – 122, 2004. |
Biological performance of red porgy (Pagrus pagrus) larvae under intensive rearing conditions with the use of an automated feeding system Journal Article Aquaculture International, 12 , pp. 191 – 203, 2004. |
Cloning, characterisation, and expression of three oestrogen receptors (ERα, ERβ1 and ERβ2) in the European sea bass, Dicentrarchus labrax Journal Article Molecular and Cellular Endocrinology, 223 (1-2), pp. 63–75, 2004, ISSN: 03037207. |
Comparison of microbial community composition from different sediments of the Eastern Mediterranean Sea using Ŧ-RFLP, DGGE and PLFA analysis Inproceedings Barcelona, Spain, 2004, (Publication Title: 37th CIESM Congress Type: Oral Presentation). |
Cryopreservation of marine microalgae and potential toxicity of cryoprotectants to the primary steps of the aquacultural food chain Journal Article Aquaculture, 230 (1-4), pp. 457–473, 2004, ISSN: 00448486, (Publisher: Elsevier). |
Deep-sea decapod crustaceans in the western and central Mediterranean Sea: preliminary aspect of species distribution, biomass and population structure. Journal Article Scientia Marina, 68 , pp. 73–86, 2004. |
Determination of oxolinic acid residues in gilthead seabream (Sparus aurata) muscle tissue and plasma by high-performance liquid chromatography Journal Article Chromatographia, 60 , pp. 657 – 661, 2004. |
Development and linkage relationships for new microsatellite markers of the sea bass (Dicentrarchus labrax L.) Journal Article Animal Genetics, 35 (1), pp. 53–57, 2004, ISSN: 02689146. |
Effect of dietary taurine supplementation on growth performance and feed selection of sea bass Dicentrarchus labrax fry fed with demand-feeders Journal Article Fisheries Science, 70 (1), pp. 74–79, 2004, ISSN: 09199268. |
Efficient isolation of polyaromatic fraction from aliphatic compounds in complex extracts using dimethylformamide-pentane partitionings Journal Article Journal of Chromatography A, 1041 (1-2), pp. 111–117, 2004, ISSN: 00219673. |
Evaluation of egg production and quality in the Mediterranean red porgy (Pagrus pagrus) during two consecutive spawning seasons Journal Article Aquaculture, 232 (1-4), pp. 637–649, 2004, ISSN: 00448486. |
Evolution of tissue composition and serum metabolites during gonadal development in the common dentex (Dentex dentex) Journal Article Aquaculture, 236 (1-4), pp. 557–573, 2004, ISSN: 00448486. |
Feeding rates, growth performance and gametogenesis of wreckfish (Polyprion americanus) kept in captivity Journal Article Aquaculture International, 12 , pp. 395 – 407, 2004. |
Induction of spawning of cultured greater amberjack (Seriola dumerili) using GnRHa implants Journal Article Aquaculture, 237 (1-4), pp. 141–154, 2004, ISSN: 00448486. |
Genome, 47 , pp. 1154–1163, 2004. |
Macronutrient selection by sharp snout seabream (Diplodus puntazzo) Journal Article Aquaculture, 232 (1-4), pp. 481–491, 2004, ISSN: 00448486. |
Mesocosm: A reliable technology for larval rearing of Diplodus puntazzo and Diplodus sargus sargus Journal Article Aquaculture International, 12 , pp. 345 – 355, 2004. |
Mitochondrial DNA Incollection Cadrin, S; Friedland, K; Waldman, J (Ed.): Stock Ιdentification Μethods, pp. 311–330, ELSEVIER Academic Press, 2004, (Backup Publisher: ELSEVIER Academic Press Section: 16). |
Pharmacokinetics of cephalexin in sea bream, Sparus aurata (L.), after a single intraperitoneal injection Journal Article Journal of Applied Ichthyology, 20 (5), pp. 422–426, 2004, ISSN: 01758659. |
Poor bioavailability of oxytetracycline in sharpsnout sea bream Diplodus puntazzo Journal Article Aquaculture, 235 , pp. 489 – 497, 2004. |
Potential drug (oxytetracycline and oxolinic acid) pollution from Mediterranean sparid fish farms Journal Article Aquatic Toxicology, 69 , pp. 281 – 288, 2004. |
Radiocarbon apportionment of fossil versus biofuel combustion sources of polycyclic aromatic hydrocarbons in the Stockholm metropolitan area Journal Article Environmental Science and Technology, 38 (20), pp. 5344–5349, 2004, ISSN: 0013936X. |
Reproductive biology of the shi drum (Umrina cirrosa) in captivity and induction of spawning using GNRHA Journal Article Israeli Journal of Aquaculture - Bamidgeh, 56 (2), pp. 77–94, 2004, ISSN: 0792156X. |
The kinetic profile of oxolinic acid in sharpsnout sea bream, Diplodus puntazzo (Cetti 1777) Journal Article Aquaculture Research, 35 , pp. 1299 – 1304, 2004. |
Wet deposition of polychlorinated biphenyls in the eastern Mediterranean Journal Article Environmental Science and Technology, 38 (11), pp. 3011–3018, 2004, ISSN: 0013936X. |
2003 |
Discord in the family Sparidae (Teleostei): divergent phylogeographical patterns across the Atlantic-Mediterranean divide Journal Article Journal of Evolutionary Biology, 16 (6), pp. 1149–1158, 2003, ISSN: 1010-061X, 1420-9101. |
The genetic architecture of correlations among growth-related traits and male age at maturation in rainbow trout Journal Article Journal of Fish Biology, 63 (3), pp. 746–764, 2003, ISSN: 0022-1112, 1095-8649. |
Computer assisted evaluation of wound healing in chronic ulcers Journal Article Computers in Biology and Medicine, 33 (4), pp. 311–317, 2003, ISSN: 00104825. |
Aquaculture, 225 (1-4), pp. 207–223, 2003, ISSN: 00448486. |
Aquaculture, 225 (1-4), pp. 309–323, 2003, ISSN: 00448486. |
Aquaculture, 225 (1-4), pp. 109–119, 2003, ISSN: 00448486. |
Inducible transgenic mice reveal resting dendritic cells as potent inducers of CD8(+) Ŧ cell tolerance Journal Article Immunity, 18 (5), pp. 713–720, 2003, (ISBN: 1074-7613). |
Seasonal patterns of spoilage of ice-stored cultured gilthead sea bream (Sparus aurata) Journal Article Food Chemistry, 81 (2), pp. 263–268, 2003, ISSN: 03088146. |
Aquaculture, 220 (1-4), pp. 633–642, 2003, ISSN: 00448486. |
Development of a saddleback-like syndrome in reared white seabream Diplodus sargus (Linnaeus, 1758) Journal Article Aquaculture, 217 (1-4), pp. 673–676, 2003, ISSN: 00448486. |
A new apparatus for the direct measurement of otter trawling effects on the epibenthic and hyperbenthic macrofauna Journal Article Journal of the Marine Biological Association of the United Kingdom, 83 (6), pp. 1363–1368, 2003, ISSN: 00253154. |
A numerical and experimental study on the hydrodynamics of a heave motion floating breakwater Inproceedings 2003, (Publication Title: XXX IAHR Congress). |
Amphipod community structure in an area of intensive natural and antropogenic disturbance (Termaicos Bay in the Mediteranean Sea). Inproceedings Tunis, Tunisia, 2003, (Publication Title: The11th International Colloquium on Amphipoda, Type: Abstract). |
An application of different bioindicators for assessing water quality: A case study in the rivers Alfeios and Pineios (Peloponnisos, Greece) Journal Article Ecological Indicators, 2 (4), pp. 345–360, 2003, ISSN: 1470160X, (Publisher: Elsevier). |
Boussinesq modeling of the alongshore sediment transport Inproceedings 2003, (Publication Title: XXX IAHR Congress). |
Direct evidence for destruction of polychlorobiphenyls by OH radicals in the subtropical troposphere Journal Article Environmental Science and Technology, 37 (3), pp. 542–547, 2003, ISSN: 0013936X. |
DNA microsatellite markers in service of swordfish stock-structure analysis in the Atlantic and Mediterranean Journal Article ICCAT Col. Vol. Sci. Papers, 55 (4), pp. 1632–1639, 2003. |
Effect of incubation experiments on the distribution of different lipid classes (aliphatic hydrocarbons, diglycerides, free fatty acids, glycolipids, phospholipids) in sediments of the Cretan Sea Inproceedings Hersonissos, Crete, Greece, 2003, (Publication Title: 7th Symposium of Oceanography & Fisheries Type: Poster). |
Eleven new microsatellites of the sea bass (Dicentrarchus labrax L.) Journal Article Molecular Ecology Notes, 3 (3), pp. 352–354, 2003, ISSN: 14718278. |