2002 |
Plaiti, W; Sardá, F; Tselepides, A The contribution of decapod crustaceans to the hyperbenthic fauna of the deep Mediterranean Sea. (poster) Inproceedings Corfu, Greece. 2-6 September., 2002, (Publication Title: The 8th Colloquium Crustacea Decapoda Mediterranea. Type: Poster). @inproceedings{plaiti_contribution_2002, title = {The contribution of decapod crustaceans to the hyperbenthic fauna of the deep Mediterranean Sea. (poster)}, author = {W Plaiti and F Sardá and A Tselepides}, year = {2002}, date = {2002-01-01}, address = {Corfu, Greece. 2-6 September.}, note = {Publication Title: The 8th Colloquium Crustacea Decapoda Mediterranea. Type: Poster}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Venizelos, L; Mouratian, Z; Apostolaki, A; Lagonica, C; Papageorgiou, Nafsika; Manoli, V The Mediterranean, a living sea - A kit for environmental education and awareness for children Inproceedings Miami, Florida, 2002, (Publication Title: 22nd Annual Symposium on Sea Turtle Biology and Conservation Type: Poster). @inproceedings{venizelos_mediterranean_2002, title = {The Mediterranean, a living sea - A kit for environmental education and awareness for children}, author = {L Venizelos and Z Mouratian and A Apostolaki and C Lagonica and Nafsika Papageorgiou and V Manoli}, year = {2002}, date = {2002-01-01}, address = {Miami, Florida}, note = {Publication Title: 22nd Annual Symposium on Sea Turtle Biology and Conservation Type: Poster}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Koutandos, E; Prinos, P Turbulent free-surface flow over a sinusoidal bed Inproceedings pp. 241–246, 2002, (Publication Title: International Conference ‘Hydroinformatics-2002). @inproceedings{koutandos_turbulent_2002, title = {Turbulent free-surface flow over a sinusoidal bed}, author = {E Koutandos and P Prinos}, year = {2002}, date = {2002-01-01}, volume = {1}, pages = {241--246}, note = {Publication Title: International Conference ‘Hydroinformatics-2002}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Kotlik, P; Tsigenopoulos, C S; Rab, P; Berrebi, P Two new Barbus species from the Danube River basin, with redescription of B. petenyi (Teleostei: Cyprinidae) Journal Article Folia Zoologica, 51 (3), pp. 227–240, 2002. @article{kotlik_two_2002, title = {Two new Barbus species from the Danube River basin, with redescription of B. petenyi (Teleostei: Cyprinidae)}, author = {P Kotlik and C S Tsigenopoulos and P Rab and P Berrebi}, year = {2002}, date = {2002-01-01}, journal = {Folia Zoologica}, volume = {51}, number = {3}, pages = {227--240}, abstract = {Three rheophilic species of western Palaearctic textitBarbus are recognised in the Danube River basin, each diagnosed by a set of unique mitochondrial DNA alleles. The three species are parapatrically distributed and morphologically similar to each other. The redescribed textitB. petenyi, from the Eastern and Southern Carpathians and Stara Planina Mts., is distinguished by a short, sharply pointed snout. textitBarbus carpathicus, new species, from the Western and Eastern Carpathians, is distinguished by a long, sharply pointed snout. textitBarbus balcanicus, new species, from the Dinaric Mts. and Western Stara Planina Mts., is distinguished by a short, bluntly pointed snout, and a unique body and fin colour pattern..}, keywords = {}, pubstate = {published}, tppubtype = {article} } Three rheophilic species of western Palaearctic textitBarbus are recognised in the Danube River basin, each diagnosed by a set of unique mitochondrial DNA alleles. The three species are parapatrically distributed and morphologically similar to each other. The redescribed textitB. petenyi, from the Eastern and Southern Carpathians and Stara Planina Mts., is distinguished by a short, sharply pointed snout. textitBarbus carpathicus, new species, from the Western and Eastern Carpathians, is distinguished by a long, sharply pointed snout. textitBarbus balcanicus, new species, from the Dinaric Mts. and Western Stara Planina Mts., is distinguished by a short, bluntly pointed snout, and a unique body and fin colour pattern.. |
Machias, Athanassios; Maraveyia, Eleni; Pavlidis, Michalis; Somarakis, Stylianos; Divanach, Pascal Validation of annuli on scales and otoliths of common dentex (Dentex dentex) Journal Article Fisheries Research, 54 (2), pp. 287–294, 2002, ISSN: 01657836. @article{machias_validation_2002, title = {Validation of annuli on scales and otoliths of common dentex (Dentex dentex)}, author = {Athanassios Machias and Eleni Maraveyia and Michalis Pavlidis and Stylianos Somarakis and Pascal Divanach}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0165783600003027}, doi = {10.1016/S0165-7836(00)00302-7}, issn = {01657836}, year = {2002}, date = {2002-01-01}, urldate = {2020-08-18}, journal = {Fisheries Research}, volume = {54}, number = {2}, pages = {287--294}, abstract = {The formation of annuli was examined from scales and otoliths of farmed (known age) common dentex, Dentex dentex. One scale annulus formed each year during May and one hyaline zone on the otoliths during June. The formation of scale annuli and the hyaline zone on otoliths of adult fish coincided with gonadal maturation which takes place during April to May. Scales were found to be more accurate than otoliths in respect to annuli formation, but their annuli were not as intense and distinct. Missing or additional hyaline zones were observed on adult otoliths. These irregularities were observed after fish maturation and were related to temperature conditions, as well as to faster growth of the farmed fish than the wild ones. © 2002 Elsevier Science B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The formation of annuli was examined from scales and otoliths of farmed (known age) common dentex, Dentex dentex. One scale annulus formed each year during May and one hyaline zone on the otoliths during June. The formation of scale annuli and the hyaline zone on otoliths of adult fish coincided with gonadal maturation which takes place during April to May. Scales were found to be more accurate than otoliths in respect to annuli formation, but their annuli were not as intense and distinct. Missing or additional hyaline zones were observed on adult otoliths. These irregularities were observed after fish maturation and were related to temperature conditions, as well as to faster growth of the farmed fish than the wild ones. © 2002 Elsevier Science B.V. All rights reserved. |
2001 |
Kotzamanis, Y P; Alexis, M N; Andriopoulou, A; Castritsi-Cathariou, I; Fotis, G Utilization of waste material resulting from trout processing in gilthead bream (Sparus aurata L.) diets Journal Article Aquaculture Research, 32 , pp. 288–295, 2001, ISSN: 1355557X, 13652109. @article{kotzamanis_utilization_2001, title = {Utilization of waste material resulting from trout processing in gilthead bream (Sparus aurata L.) diets}, author = {Y P Kotzamanis and M N Alexis and A Andriopoulou and I Castritsi-Cathariou and G Fotis}, url = {http://doi.wiley.com/10.1046/j.1355-557x.2001.00042.x}, doi = {10.1046/j.1355-557x.2001.00042.x}, issn = {1355557X, 13652109}, year = {2001}, date = {2001-12-01}, urldate = {2020-08-18}, journal = {Aquaculture Research}, volume = {32}, pages = {288--295}, abstract = {Fish processing creates a large amount of waste of high nutrient content which, if not properly processed for use in human or animal nutrition, is likely to be deposited in the environment creating pollution problems. Waste parts from rainbow trout processing for smoking, consisting of heads, bones, tails and intestines, were used as feed ingredients for gilthead bream diets. Heads, bones and tails had similar compositions, their weighed mean indicating about 700 g kg-1 moisture, 150 g kg-1 protein and 110 g kg-1 fat. Intestines contained higher lipid (350 g kg-1) and lower moisture (560 g kg-1) and protein content (80 g kg-1). Seasonal changes in composition indicated significant differences. Three experimental diets were formulated having the same proximate composition on a dry weight basis. The control diet (A) contained fish meal as the main protein source and fish oil as the oil supplement. In diet B part of the protein and most of lipid was provided by trout waste and in diet C most of the lipid was provided by trout intestines. Gilthead bream fingerlings of 4 g initial weight were fed to apparent satiation for 72 days, at a temperature of 20°C, to an average final weight of 19 g. All diets were fed in a dry form. The experiment was performed in duplicate. Growth and feed utilization data were high and similar among groups. The body composition of the resulting fish did not show any difference among dietary treatments. Differences in liver lipid and fatty acid content were found between all dietary treatments. The growth and body composition data from this preliminary experiment indicated that trout waste could be used successfully as a dietary ingredient of sea bream diets. © 2001 Blackwell Science Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Fish processing creates a large amount of waste of high nutrient content which, if not properly processed for use in human or animal nutrition, is likely to be deposited in the environment creating pollution problems. Waste parts from rainbow trout processing for smoking, consisting of heads, bones, tails and intestines, were used as feed ingredients for gilthead bream diets. Heads, bones and tails had similar compositions, their weighed mean indicating about 700 g kg-1 moisture, 150 g kg-1 protein and 110 g kg-1 fat. Intestines contained higher lipid (350 g kg-1) and lower moisture (560 g kg-1) and protein content (80 g kg-1). Seasonal changes in composition indicated significant differences. Three experimental diets were formulated having the same proximate composition on a dry weight basis. The control diet (A) contained fish meal as the main protein source and fish oil as the oil supplement. In diet B part of the protein and most of lipid was provided by trout waste and in diet C most of the lipid was provided by trout intestines. Gilthead bream fingerlings of 4 g initial weight were fed to apparent satiation for 72 days, at a temperature of 20°C, to an average final weight of 19 g. All diets were fed in a dry form. The experiment was performed in duplicate. Growth and feed utilization data were high and similar among groups. The body composition of the resulting fish did not show any difference among dietary treatments. Differences in liver lipid and fatty acid content were found between all dietary treatments. The growth and body composition data from this preliminary experiment indicated that trout waste could be used successfully as a dietary ingredient of sea bream diets. © 2001 Blackwell Science Ltd. |
Koumoundouros, G; Sfakianakis, D; Maingot, E; Divanach, P; Kentouri, M Osteological development of the vertebral column and of the fins in Diplodus sargus (Teleostei: Perciformes: Sparidae) Journal Article Marine Biology, 139 (5), pp. 853 – 862, 2001, ISSN: 0025-3162, 1432-1793. @article{koumoundouros_osteological_2001, title = {Osteological development of the vertebral column and of the fins in Diplodus sargus (Teleostei: Perciformes: Sparidae)}, author = {G Koumoundouros and D Sfakianakis and E Maingot and P Divanach and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035654760&partnerID=40&md5=cc6c9efa1262f94c00bbb1f34b0fc21d}, doi = {10.1007/s002270100645}, issn = {0025-3162, 1432-1793}, year = {2001}, date = {2001-11-01}, journal = {Marine Biology}, volume = {139}, number = {5}, pages = {853 -- 862}, abstract = {The osteological development of vertebral column and fins in white sea bream, Diplodus sargus (Linnaeus, 1758) was studied. Vertebral ontogeny started at 5.7 mm total length (TL, post-fixation), with the formation of the first cartilaginous neural and haemal arches, and was completed by the full attainment of dorsal ribs (20.8 mm TL). The formation of vertebral centra occurred between 7.3 and 9.4 mm TL, but was fully completed only after the development of post- and pre-zygapophyses (26.0 mm TL). Pectoral supports were the first fin elements that started to develop (3.8 mm TL), followed by those of the caudal fin (5.2 mm TL), dorsal and anal fins (6.7 mm TL), and then by those of the pelvic fin (9.5 mm TL). The caudal fin of D. sargus was the first to develop fin rays and attain the full count of lepidotrichia (5.6-7.8 mm TL), but the last to attain the full count of dermatotrichia (7.9-16.0 mm TL). The next fins starting to present rays were the pectoral (6.5 mm TL) and the dorsal and anal fins (7.5 mm TL), while the pelvic fins were the last (11.5 mm TL). Following the caudal lepidotrichia (7.8 mm TL), the anal (10.5 mm TL), dorsal (12.0 mm TL), pectoral (11.4 mm TL) and pelvic fins (12.5 mm TL) were the next with fully completed ray counts. Finally, fin meristics were fully developed with the caudal dermatotrichia. The results are discussed in comparison with the fin and vertebral ontogeny of other teleosts.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The osteological development of vertebral column and fins in white sea bream, Diplodus sargus (Linnaeus, 1758) was studied. Vertebral ontogeny started at 5.7 mm total length (TL, post-fixation), with the formation of the first cartilaginous neural and haemal arches, and was completed by the full attainment of dorsal ribs (20.8 mm TL). The formation of vertebral centra occurred between 7.3 and 9.4 mm TL, but was fully completed only after the development of post- and pre-zygapophyses (26.0 mm TL). Pectoral supports were the first fin elements that started to develop (3.8 mm TL), followed by those of the caudal fin (5.2 mm TL), dorsal and anal fins (6.7 mm TL), and then by those of the pelvic fin (9.5 mm TL). The caudal fin of D. sargus was the first to develop fin rays and attain the full count of lepidotrichia (5.6-7.8 mm TL), but the last to attain the full count of dermatotrichia (7.9-16.0 mm TL). The next fins starting to present rays were the pectoral (6.5 mm TL) and the dorsal and anal fins (7.5 mm TL), while the pelvic fins were the last (11.5 mm TL). Following the caudal lepidotrichia (7.8 mm TL), the anal (10.5 mm TL), dorsal (12.0 mm TL), pectoral (11.4 mm TL) and pelvic fins (12.5 mm TL) were the next with fully completed ray counts. Finally, fin meristics were fully developed with the caudal dermatotrichia. The results are discussed in comparison with the fin and vertebral ontogeny of other teleosts. |
Koumoundouros, G; Divanach, P; Anezaki, L; Kentouri, M Temperature-induced ontogenetic plasticity in sea bass (Dicentrarchus labrax) Journal Article Marine Biology, 139 (5), pp. 817 – 830, 2001, ISSN: 0025-3162, 1432-1793. @article{koumoundouros_temperature-induced_2001, title = {Temperature-induced ontogenetic plasticity in sea bass (Dicentrarchus labrax)}, author = {G Koumoundouros and P Divanach and L Anezaki and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035651680&partnerID=40&md5=c0b2c440d241826ebd72b40d6ade80f2}, doi = {10.1007/s002270100635}, issn = {0025-3162, 1432-1793}, year = {2001}, date = {2001-11-01}, journal = {Marine Biology}, volume = {139}, number = {5}, pages = {817 -- 830}, abstract = {We studied the ontogeny of Dicentrarchus labrax comparatively under constant rearing temperatures of 13, 15 and 20°C. At hatching, yolk-sac larval morphometry differed significantly between the temperatures and especially between the two extremes, while at the end of the yolk-sac larval stage, it mainly differed between the two lower temperature regimes and that of 20°C. Compared with the two lower temperature conditions, at 20°C D. labrax presented a significant ontogenetic acceleration which was morphologically expressed either as a significantly smaller total length (TL) at feeding onset, notochord flexion and fin differentiation, or as shifts of the allometric inflection points of 8 out of the 15 morphometric characters studied. Additionally, temperature significantly affected the allometry coefficients, with a decreasing growth intensity as the temperature difference decreased. The rate of TL growth increased under elevated temperature conditions throughout the entire ontogenetic period, except during the early larval period (feeding onset to metamorphosis onset), at which time D. labrax presented equal growth rates at 15°C and 20°C. The results are discussed with respect to the ontogeny of the functional morphology and the meanings of temperature- induced ontogenetic plasticity for the survival of fish larvae.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We studied the ontogeny of Dicentrarchus labrax comparatively under constant rearing temperatures of 13, 15 and 20°C. At hatching, yolk-sac larval morphometry differed significantly between the temperatures and especially between the two extremes, while at the end of the yolk-sac larval stage, it mainly differed between the two lower temperature regimes and that of 20°C. Compared with the two lower temperature conditions, at 20°C D. labrax presented a significant ontogenetic acceleration which was morphologically expressed either as a significantly smaller total length (TL) at feeding onset, notochord flexion and fin differentiation, or as shifts of the allometric inflection points of 8 out of the 15 morphometric characters studied. Additionally, temperature significantly affected the allometry coefficients, with a decreasing growth intensity as the temperature difference decreased. The rate of TL growth increased under elevated temperature conditions throughout the entire ontogenetic period, except during the early larval period (feeding onset to metamorphosis onset), at which time D. labrax presented equal growth rates at 15°C and 20°C. The results are discussed with respect to the ontogeny of the functional morphology and the meanings of temperature- induced ontogenetic plasticity for the survival of fish larvae. |
I., Leonardos; P., Katharios; Charisis, C Age and growth of Carassius auratus gibelio (Linnaeus, 1758) (Pisces: Cyprinidae) in Lake Lysimachia (W. Greece). Inproceedings of Ichthyologists, Panhellenic Society (Ed.): 10th Panhellenic Conference of Ichthyologists, pp. 257–259, Chania, Greece, 2001. @inproceedings{leonardos_age_2001, title = {Age and growth of Carassius auratus gibelio (Linnaeus, 1758) (Pisces: Cyprinidae) in Lake Lysimachia (W. Greece).}, author = {Leonardos I. and Katharios P. and C Charisis}, editor = {Panhellenic Society of Ichthyologists}, year = {2001}, date = {2001-10-01}, booktitle = {10th Panhellenic Conference of Ichthyologists}, pages = {257--259}, address = {Chania, Greece}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Koumoundouros, G; Divanach, P; Kentouri, M The effect of rearing conditions on development of saddleback syndrome and caudal fin deformities in Dentex dentex (L.) Journal Article Aquaculture, 200 (3-4), pp. 285–304, 2001, ISSN: 00448486. @article{koumoundouros_effect_2001, title = {The effect of rearing conditions on development of saddleback syndrome and caudal fin deformities in Dentex dentex (L.)}, author = {G Koumoundouros and P Divanach and M Kentouri}, url = {https://linkinghub.elsevier.com/retrieve/pii/S004484860100552X}, doi = {10.1016/S0044-8486(01)00552-X}, issn = {00448486}, year = {2001}, date = {2001-09-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {200}, number = {3-4}, pages = {285--304}, abstract = {The development of saddleback syndrome and of caudal fin deformities in Dentex dentex (Linnaeus, 1758) was compared under two rearing methods, extensive (E) and semi-extensive (S). The osteological appearance and the meristic characters of the reared fish were compared to that of D. dentex juveniles, collected from the natural environment. All the wild juveniles were normal in respect to their osteological appearance, while the reared specimens presented skeletal deformities resulting from different rearing methods applied. Saddleback syndrome (4.0-4.4%) and severe external deformities of the caudal fin (14.3-15.0%) characterised exclusively the semi-extensive populations, while the extensive populations presented severe abnormalities of the pre-ural centra (25.0-25.6%) with significantly higher frequency than the S reared fish (5.8%). Saddleback syndrome was expressed as a lack of one to all the hard spines of the dorsal fin, accompanied by shape, number and position abnormalities of the related pterygiophores. Caudal fin deformities were mainly characterised by the lack of the upper lepidotrichia or/and dermatotrichia, accompanied by severe deformities of the supporting elements of the upper lobe. The saddleback syndrome and severe caudal fin deformities were anatomically and ontogenetically related to each other, originating at the early larval stage as a result of abnormalities of the primordial marginal finfold and of the posterior tip of the notochord. In respect to the dominant phenotypes, the meristic characters of the normal reared fish were not differentiated from those of the wild, but they presented higher variability. The results are discussed in view of the aetiology, applications and research targets for the erasure of skeletal malformations. © 2001 Elsevier Science B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The development of saddleback syndrome and of caudal fin deformities in Dentex dentex (Linnaeus, 1758) was compared under two rearing methods, extensive (E) and semi-extensive (S). The osteological appearance and the meristic characters of the reared fish were compared to that of D. dentex juveniles, collected from the natural environment. All the wild juveniles were normal in respect to their osteological appearance, while the reared specimens presented skeletal deformities resulting from different rearing methods applied. Saddleback syndrome (4.0-4.4%) and severe external deformities of the caudal fin (14.3-15.0%) characterised exclusively the semi-extensive populations, while the extensive populations presented severe abnormalities of the pre-ural centra (25.0-25.6%) with significantly higher frequency than the S reared fish (5.8%). Saddleback syndrome was expressed as a lack of one to all the hard spines of the dorsal fin, accompanied by shape, number and position abnormalities of the related pterygiophores. Caudal fin deformities were mainly characterised by the lack of the upper lepidotrichia or/and dermatotrichia, accompanied by severe deformities of the supporting elements of the upper lobe. The saddleback syndrome and severe caudal fin deformities were anatomically and ontogenetically related to each other, originating at the early larval stage as a result of abnormalities of the primordial marginal finfold and of the posterior tip of the notochord. In respect to the dominant phenotypes, the meristic characters of the normal reared fish were not differentiated from those of the wild, but they presented higher variability. The results are discussed in view of the aetiology, applications and research targets for the erasure of skeletal malformations. © 2001 Elsevier Science B.V. All rights reserved. |
Palma, J; Alarcon, J A; Alvarez, C; Zouros, E; Magoulas, A; Andrade, J P Developmental stability and genetic heterozygosity in wild and cultured stocks of gilthead sea bream (Sparus aurata) Journal Article Journal of the Marine Biological Association of the United Kingdom, 81 (2), pp. 283–288, 2001, ISSN: 0025-3154, 1469-7769. @article{palma_developmental_2001, title = {Developmental stability and genetic heterozygosity in wild and cultured stocks of gilthead sea bream (Sparus aurata)}, author = {J Palma and J A Alarcon and C Alvarez and E Zouros and A Magoulas and J P Andrade}, url = {https://www.cambridge.org/core/product/identifier/S0025315401003757/type/journal_article}, doi = {10.1017/S0025315401003757}, issn = {0025-3154, 1469-7769}, year = {2001}, date = {2001-04-01}, urldate = {2020-08-09}, journal = {Journal of the Marine Biological Association of the United Kingdom}, volume = {81}, number = {2}, pages = {283--288}, abstract = {The present study checks on the validity of the hypothesis that heterozygosity and the fluctuating asymmetry (FA), common measure of the developmental stability, are linked in populations of wild and cultured stocks of Sparus aurata from five countries. Muscle and liver samples were analysed for variation in 26 allozymes and three microsatellite loci. Pectoral fin rays and upper and lower gill rakers of the first branchial arch were counted on the left and on the right sides of each fish. Fluctuating asymmetry existed in the majority of the samples although their values were consistently low, (0·305±0·147), but higher in the cultured samples. The allozyme heterozygosity values were always high, but lower in the cultured samples. The microsatellite DNA analysis produced similar results. Heterozygosity was higher in cultured individuals (except for the Greek samples). These findings seem to be early evidence that the reared samples are losing some genetic variation, especially due to the loss of the rarest alleles (which were present in the wild populations). Genetic drift, probably caused by propagation practices, is most likely responsible for the decrease of the genetic variation. No distinct pattern of geographic separation was identified.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The present study checks on the validity of the hypothesis that heterozygosity and the fluctuating asymmetry (FA), common measure of the developmental stability, are linked in populations of wild and cultured stocks of Sparus aurata from five countries. Muscle and liver samples were analysed for variation in 26 allozymes and three microsatellite loci. Pectoral fin rays and upper and lower gill rakers of the first branchial arch were counted on the left and on the right sides of each fish. Fluctuating asymmetry existed in the majority of the samples although their values were consistently low, (0·305±0·147), but higher in the cultured samples. The allozyme heterozygosity values were always high, but lower in the cultured samples. The microsatellite DNA analysis produced similar results. Heterozygosity was higher in cultured individuals (except for the Greek samples). These findings seem to be early evidence that the reared samples are losing some genetic variation, especially due to the loss of the rarest alleles (which were present in the wild populations). Genetic drift, probably caused by propagation practices, is most likely responsible for the decrease of the genetic variation. No distinct pattern of geographic separation was identified. |
Navajas, M; Thistlewood, H; Lagnel, J; Marshall, D; Tsagkarakou, A; Pasteur, N Biological Control, 20 (3), pp. 191–198, 2001, ISSN: 10499644. @article{navajas_field_2001, title = {Field releases of the predatory mite Neoseiulus fallacis (Acari : Phytoseiidae) in Canada, monitored by pyrethroid resistance and allozyme markers}, author = {M Navajas and H Thistlewood and J Lagnel and D Marshall and A Tsagkarakou and N Pasteur}, url = {https://linkinghub.elsevier.com/retrieve/pii/S104996440090896X}, doi = {10.1006/bcon.2000.0896}, issn = {10499644}, year = {2001}, date = {2001-03-01}, urldate = {2020-08-18}, journal = {Biological Control}, volume = {20}, number = {3}, pages = {191--198}, abstract = {The predacious phytoseiid mite Neoseiulus fallacis (Garman) is an important agent for the biological control of spider mites in deciduous fruit orchards in North America and Canada. It would be helpful to monitor the fate of released individuals to improve the results of introductions of the predators in biological control trials. We have used two types of genetic markers, pyrethroid resistance and allozymes, for indirect estimation of the survival of N. fallacis introduced in an apple orchard in Ontario, Canada. Mite samples were submitted to toxicological tests. The polymorphism of four enzymes was studied in individual females using an isoelectric focusing technique. A mite sample was taken from the field, mass-reared in the laboratory, and selected for permethrin resistance. This strain was released on several apple trees treated with permethrin, and mite samples were collected from the same trees 10 to 90 days later. The genetic composition and the insecticide resistance level of this sample were compared to those of two other samples from trees where mites had not previously been released, either in the same orchard or in a neighboring block. A control susceptible strain was compared using mites collected earlier from trees on the same site but outside the present experiment. The mites collected from the release trees and those from the strain used for the releases were found to be genetically closely related, as judged from a small genetic distance, and from similar levels of insecticide resistance in both samples. The control samples from the nonrelease trees were genetically distant from these and displayed low resistance levels. These results indicate that the released genotypes established and persisted in the release trees for the period of the experiment. The utility of the two approaches in assessing the fate of released natural enemies is discussed. (C) 2001 Academic Press.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The predacious phytoseiid mite Neoseiulus fallacis (Garman) is an important agent for the biological control of spider mites in deciduous fruit orchards in North America and Canada. It would be helpful to monitor the fate of released individuals to improve the results of introductions of the predators in biological control trials. We have used two types of genetic markers, pyrethroid resistance and allozymes, for indirect estimation of the survival of N. fallacis introduced in an apple orchard in Ontario, Canada. Mite samples were submitted to toxicological tests. The polymorphism of four enzymes was studied in individual females using an isoelectric focusing technique. A mite sample was taken from the field, mass-reared in the laboratory, and selected for permethrin resistance. This strain was released on several apple trees treated with permethrin, and mite samples were collected from the same trees 10 to 90 days later. The genetic composition and the insecticide resistance level of this sample were compared to those of two other samples from trees where mites had not previously been released, either in the same orchard or in a neighboring block. A control susceptible strain was compared using mites collected earlier from trees on the same site but outside the present experiment. The mites collected from the release trees and those from the strain used for the releases were found to be genetically closely related, as judged from a small genetic distance, and from similar levels of insecticide resistance in both samples. The control samples from the nonrelease trees were genetically distant from these and displayed low resistance levels. These results indicate that the released genotypes established and persisted in the release trees for the period of the experiment. The utility of the two approaches in assessing the fate of released natural enemies is discussed. (C) 2001 Academic Press. |
Cotou, E; Castritsi-Catharios, I; Moraitou-Apostolopoulou, M Surfactant-based oil dispersant toxicity to developing nauplii of Artemia: Effects on ATPase enzymatic system Journal Article Chemosphere, 42 (8), pp. 959–964, 2001, ISSN: 00456535. @article{cotou_surfactant-based_2001, title = {Surfactant-based oil dispersant toxicity to developing nauplii of Artemia: Effects on ATPase enzymatic system}, author = {E Cotou and I Castritsi-Catharios and M Moraitou-Apostolopoulou}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0045653500001089}, doi = {10.1016/S0045-6535(00)00108-9}, issn = {00456535}, year = {2001}, date = {2001-03-01}, urldate = {2020-08-18}, journal = {Chemosphere}, volume = {42}, number = {8}, pages = {959--964}, abstract = {The paper deals with the toxicity of a surfactant-based oil dispersant to the ATPase activities of two naupliar stages of Artemia (instar I & II). Both instars were exposed to sub-lethal and lethal concentrations derived from acute toxicity data. The chosen concentrations were near to LOECs and NOECs. An eightfold difference indicated between the instars was instar-exposure time dependent. The most prominent effects were the inhibition and the stimulation of Na+/K+-ATPase and Mg2+-ATPase activities, respectively. The cause of these effects was related to the dispersant components, the surfactants. The pattern stimulation/inhibition of Mg2+-ATPase and Na+/K+-ATPase activities could be used to indicate toxic stress by surfactant-based oil dispersants since previous studies with other contaminants have shown different ATPase activity patterns. © 2001 Elsevier Science Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The paper deals with the toxicity of a surfactant-based oil dispersant to the ATPase activities of two naupliar stages of Artemia (instar I & II). Both instars were exposed to sub-lethal and lethal concentrations derived from acute toxicity data. The chosen concentrations were near to LOECs and NOECs. An eightfold difference indicated between the instars was instar-exposure time dependent. The most prominent effects were the inhibition and the stimulation of Na+/K+-ATPase and Mg2+-ATPase activities, respectively. The cause of these effects was related to the dispersant components, the surfactants. The pattern stimulation/inhibition of Mg2+-ATPase and Na+/K+-ATPase activities could be used to indicate toxic stress by surfactant-based oil dispersants since previous studies with other contaminants have shown different ATPase activity patterns. © 2001 Elsevier Science Ltd. |
Koumoundouros, G; Divanach, P; Kentouri, M Osteological development of Dentex dentex (Osteichthyes: Sparidae): dorsal, anal, paired fins and squamation Journal Article Marine Biology, 138 (2), pp. 399–406, 2001, ISSN: 0025-3162, 1432-1793. @article{koumoundouros_osteological_2001-1, title = {Osteological development of Dentex dentex (Osteichthyes: Sparidae): dorsal, anal, paired fins and squamation}, author = {G Koumoundouros and P Divanach and M Kentouri}, url = {http://link.springer.com/10.1007/s002270000460}, doi = {10.1007/s002270000460}, issn = {0025-3162, 1432-1793}, year = {2001}, date = {2001-02-01}, urldate = {2020-08-18}, journal = {Marine Biology}, volume = {138}, number = {2}, pages = {399--406}, abstract = {The osteological development of dorsal, anal and paired fins and the squamation in common dentex, Dentex dentex (Linnaeus, 1758), was studied under extensive larval rearing conditions. The ontogeny of the fins was determined in three stages: (1) the yolk-sac and the early larval stage (up to 6.7 mm in vivo total length, TL), in which the majority of the elements of the pectoral fins and the predorsals, dorsal and anal proximal pterygiophores formed; (2) the late larval stage (up to 11.6 mm TL), in which the majority of the pectoral lepidotrichia, the lower extrascapulae, all the elements of the pelvic fins, as well as the lepidotrichia, spines and the distal radials of the rest fins developed; and (3) the metamorphosis stage (up to 24.0 mm TL), when the upper extrascapulae formed and the pectoral lepidotrichia attained their full count. The ontogeny of squamation was carried out during the metamorphosis stage; it started at 13.4 mm TL with laterally symmetric points on the middle of each side of the body, followed by one ventral centre (15.6 mm TL) which was located anterior to the pelvic fins, and two bilateral pairs of centres which appeared on the head (17.8 and 21.2 mm TL). The results are discussed from a functional viewpoint and in comparison with the fin and scale ontogeny of other teleosts.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The osteological development of dorsal, anal and paired fins and the squamation in common dentex, Dentex dentex (Linnaeus, 1758), was studied under extensive larval rearing conditions. The ontogeny of the fins was determined in three stages: (1) the yolk-sac and the early larval stage (up to 6.7 mm in vivo total length, TL), in which the majority of the elements of the pectoral fins and the predorsals, dorsal and anal proximal pterygiophores formed; (2) the late larval stage (up to 11.6 mm TL), in which the majority of the pectoral lepidotrichia, the lower extrascapulae, all the elements of the pelvic fins, as well as the lepidotrichia, spines and the distal radials of the rest fins developed; and (3) the metamorphosis stage (up to 24.0 mm TL), when the upper extrascapulae formed and the pectoral lepidotrichia attained their full count. The ontogeny of squamation was carried out during the metamorphosis stage; it started at 13.4 mm TL with laterally symmetric points on the middle of each side of the body, followed by one ventral centre (15.6 mm TL) which was located anterior to the pelvic fins, and two bilateral pairs of centres which appeared on the head (17.8 and 21.2 mm TL). The results are discussed from a functional viewpoint and in comparison with the fin and scale ontogeny of other teleosts. |
Makridis, P; Bergh, Ã; Skjermo, J; Vadstein, O Addition of bacteria bioencapsulated in Artemia metanauplii to a rearing system for halibut larvae Journal Article Aquaculture International, 9 , pp. 225 – 235, 2001. @article{makridis_addition_2001, title = {Addition of bacteria bioencapsulated in Artemia metanauplii to a rearing system for halibut larvae}, author = {P Makridis and à Bergh and J Skjermo and O Vadstein}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035741645&partnerID=40&md5=e90e6486f7d68ec62f82abaa5bb80066}, year = {2001}, date = {2001-01-01}, journal = {Aquaculture International}, volume = {9}, pages = {225 -- 235}, abstract = {The aim of this study was to determine whether it is possible to influence the species composition of the gut microflora in 70-days old halibut larvae (Hippoglossus hippoglossus L.) by addition of bacteria bioencapsulated in Artemia franciscana in two short pulses. Two Vibrio strains, PB 1-11 and PB 6-1 were used. Two treatments received each strain alone, a third treatment received the two bacterial strains in mixture, whereas in a control treatment no bacteria were added. Five fish tanks were included in each treatment. The bacteria were bioencapsulated in Artemia franciscana metanauplii during a short-term incubation in bacterial suspensions of the specific strains, and were added in two pulses, on day 1 and day 10 of the experiment. Addition of bacteria did not increase the total number of colony-forming units (CFU) in the larval gut. The total CFU in the water was lower in tanks added bioencapsulated bacteria than in the control treatment (p textless 0.05). An immunocolony blot assay was used to measure the numbers of the specific bacteria added in samples of water, A. franciscana, and fish larvae. At the end of the experiment on day 13, the strain PB 1-11 reached on average 10% of the total CFU when added alone and 25% when added in mixture with PB 6-1. Strain PB 6-1 reached at the end of the experiment 26.8% of the total CFU when added alone and 24.2% when added in mixture with PB 1-11, whereas the percentages of PB 1-11 and PB 6-1 in the control fish were 12.7% and 10.8% of the total CFU, respectively. However, the differences between the experimental treatments compared with the control group were not significant (p textgreater 0.05). The addition of bioencapsulated bacteria in two pulses within a ten days period was therefore not sufficient to influence the species composition of the microflora of the halibut larvae.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The aim of this study was to determine whether it is possible to influence the species composition of the gut microflora in 70-days old halibut larvae (Hippoglossus hippoglossus L.) by addition of bacteria bioencapsulated in Artemia franciscana in two short pulses. Two Vibrio strains, PB 1-11 and PB 6-1 were used. Two treatments received each strain alone, a third treatment received the two bacterial strains in mixture, whereas in a control treatment no bacteria were added. Five fish tanks were included in each treatment. The bacteria were bioencapsulated in Artemia franciscana metanauplii during a short-term incubation in bacterial suspensions of the specific strains, and were added in two pulses, on day 1 and day 10 of the experiment. Addition of bacteria did not increase the total number of colony-forming units (CFU) in the larval gut. The total CFU in the water was lower in tanks added bioencapsulated bacteria than in the control treatment (p textless 0.05). An immunocolony blot assay was used to measure the numbers of the specific bacteria added in samples of water, A. franciscana, and fish larvae. At the end of the experiment on day 13, the strain PB 1-11 reached on average 10% of the total CFU when added alone and 25% when added in mixture with PB 6-1. Strain PB 6-1 reached at the end of the experiment 26.8% of the total CFU when added alone and 24.2% when added in mixture with PB 1-11, whereas the percentages of PB 1-11 and PB 6-1 in the control fish were 12.7% and 10.8% of the total CFU, respectively. However, the differences between the experimental treatments compared with the control group were not significant (p textgreater 0.05). The addition of bioencapsulated bacteria in two pulses within a ten days period was therefore not sufficient to influence the species composition of the microflora of the halibut larvae. |
Triantafyllou, G; Petihakis, George; Dounas, C; Theodorou, A Assessing marine ecosystem response to nutrient inputs Journal Article Marine Pollution Bulletin, 43 , pp. 175–186, 2001. @article{triantafyllou_assessing_2001, title = {Assessing marine ecosystem response to nutrient inputs}, author = {G Triantafyllou and George Petihakis and C Dounas and A Theodorou}, year = {2001}, date = {2001-01-01}, journal = {Marine Pollution Bulletin}, volume = {43}, pages = {175--186}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Parpoura, A C R; Alexis, M N Effects of different dietary oils in sea bass (Dicentrarchus labrax) nutrition Journal Article Aquaculture International, 9 , pp. 463 – 476, 2001. @article{parpoura_effects_2001, title = {Effects of different dietary oils in sea bass (Dicentrarchus labrax) nutrition}, author = {A C R Parpoura and M N Alexis}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035779783&partnerID=40&md5=f2693ea4761f47fdbe16fdc2575c7510}, doi = {https://doi.org/10.1023/A:1020590701325}, year = {2001}, date = {2001-01-01}, journal = {Aquaculture International}, volume = {9}, pages = {463 -- 476}, abstract = {Five diets having the same proximate composition but containing different types of supplemental oils, singly or in combination, were used for growing sea bass from 95 g to about 200 g in small sea cages. The oils tested were olive oil, soybean oil and fish oil. The diets formulated contained EPA and DHA levels ranging from 0.88 to 1.35% of the diet. Growth parameters and fish body composition were not significantly affected by the type of oil used. The same was generally apparent for liver and hematological characteristics. The content of phospholipids in EPA and DHA was highest in the livers of fish fed diets supplemented with fish oil. A positive correlation was found between dietary and liver n-6 PUFA. Histological sections indicated extended pathological symptoms (intensive liver degeneration and hemorrhages, changes in the gill structure) in the fish receiving diets supplemented only with plant oils. These symptoms existed but to a smaller frequency and degree in livers of fish fed diets supplemented with plant and fish oil, while were not apparent in those fed the fish oil diet.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Five diets having the same proximate composition but containing different types of supplemental oils, singly or in combination, were used for growing sea bass from 95 g to about 200 g in small sea cages. The oils tested were olive oil, soybean oil and fish oil. The diets formulated contained EPA and DHA levels ranging from 0.88 to 1.35% of the diet. Growth parameters and fish body composition were not significantly affected by the type of oil used. The same was generally apparent for liver and hematological characteristics. The content of phospholipids in EPA and DHA was highest in the livers of fish fed diets supplemented with fish oil. A positive correlation was found between dietary and liver n-6 PUFA. Histological sections indicated extended pathological symptoms (intensive liver degeneration and hemorrhages, changes in the gill structure) in the fish receiving diets supplemented only with plant oils. These symptoms existed but to a smaller frequency and degree in livers of fish fed diets supplemented with plant and fish oil, while were not apparent in those fed the fish oil diet. |
Garrigues, P; Narbonne, J F; Budzinski, H; Viarengo, A; Lehtonen, K; Bocquene, G; Anderen, O K; Peters, L; Lowe, D; Cajaraville, M P; Cotou, E; Minier, C; Auffret, M; Rahmani, R Effets biologiques de la pollution environmentale dans les ecosystemes marins cotiers Journal Article Journal de Recherche Océanographique, 26 (3), pp. 31–34, 2001. @article{garrigues_effets_2001, title = {Effets biologiques de la pollution environmentale dans les ecosystemes marins cotiers}, author = {P Garrigues and J F Narbonne and H Budzinski and A Viarengo and K Lehtonen and G Bocquene and O K Anderen and L Peters and D Lowe and M P Cajaraville and E Cotou and C Minier and M Auffret and R Rahmani}, year = {2001}, date = {2001-01-01}, journal = {Journal de Recherche Océanographique}, volume = {26}, number = {3}, pages = {31--34}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Zohar, Y; Mylonas, C C Endocrine manipulations of spawning in cultured fish: From hormones to genes Journal Article Aquaculture, 197 (1-4), pp. 99–136, 2001, ISSN: 00448486. @article{zohar_endocrine_2001, title = {Endocrine manipulations of spawning in cultured fish: From hormones to genes}, author = {Y Zohar and C C Mylonas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035371765&doi=10.1016%2fS0044-8486%2801%2900584-1&partnerID=40&md5=a0f675c73f277d13cecaf3b092505c8c}, doi = {10.1016/S0044-8486(01)00584-1}, issn = {00448486}, year = {2001}, date = {2001-01-01}, journal = {Aquaculture}, volume = {197}, number = {1-4}, pages = {99--136}, abstract = {Almost all fish reared in captivity exhibit some form of reproductive dysfunction. In females, there is often failure to undergo final oocyte maturation, ovulation and spawning; while in males milt production may be reduced and of low quality. These dysfunctions are due to the fact that fish in captivity do not experience the conditions of the spawning grounds, and as a result there is a failure of the pituitary to release the maturational gonadotropin, luteinizing hormone (LH). Reproductive hormones have been utilized since the 1930s to stimulate reproductive processes and induce ovulation/spermiation and spawning. The first methods employed freshly ground pituitaries collected from reproductively mature fish, which contained gonadotropins (mainly LH) and induced steroidogenesis and gonadal maturation. Eventually, purified gonadotropins became available, both of piscine and mammalian origin, e.g., carp or salmon gonadotropin, and human chorionic gonadotropin. In the 1970s, spawning induction methods begun employing the newly discovered gonadotropin-releasing hormone (GnRH), which induces the secretion of the fish's own gonadotropin from the pituitary, thereby overcoming the endocrine failure observed in captive broodstocks. Development of highly potent, synthetic agonists of GnRH (GnRHa) constituted the next generation of hormonal manipulation therapies, and created a surge in the use of hormones to control reproductive processes in aquaculture. The most recent development is the incorporation of GnRHa into polymeric sustained-release delivery systems, which release the hormone over a period of days to weeks. These delivery systems alleviate the need for multiple treatments and induce (a) long-term elevation in sperm production and (b) multiple spawning in fish with asynchronous or multiple-batch group-synchronous ovarian physiology. Based on the recent discovery of GnRH multiplicity in fish and the increasing understanding of its functional significance, new GnRH agonists can be designed for more potent, affordable and physiologically-compatible spawning induction therapies. Future strategies for improved spawning manipulations will be based on understanding the captivity-induced alterations in the GnRH system, and on new approaches for their repair at the level of GnRH gene expression and release. © 2001 Elsevier Science B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Almost all fish reared in captivity exhibit some form of reproductive dysfunction. In females, there is often failure to undergo final oocyte maturation, ovulation and spawning; while in males milt production may be reduced and of low quality. These dysfunctions are due to the fact that fish in captivity do not experience the conditions of the spawning grounds, and as a result there is a failure of the pituitary to release the maturational gonadotropin, luteinizing hormone (LH). Reproductive hormones have been utilized since the 1930s to stimulate reproductive processes and induce ovulation/spermiation and spawning. The first methods employed freshly ground pituitaries collected from reproductively mature fish, which contained gonadotropins (mainly LH) and induced steroidogenesis and gonadal maturation. Eventually, purified gonadotropins became available, both of piscine and mammalian origin, e.g., carp or salmon gonadotropin, and human chorionic gonadotropin. In the 1970s, spawning induction methods begun employing the newly discovered gonadotropin-releasing hormone (GnRH), which induces the secretion of the fish's own gonadotropin from the pituitary, thereby overcoming the endocrine failure observed in captive broodstocks. Development of highly potent, synthetic agonists of GnRH (GnRHa) constituted the next generation of hormonal manipulation therapies, and created a surge in the use of hormones to control reproductive processes in aquaculture. The most recent development is the incorporation of GnRHa into polymeric sustained-release delivery systems, which release the hormone over a period of days to weeks. These delivery systems alleviate the need for multiple treatments and induce (a) long-term elevation in sperm production and (b) multiple spawning in fish with asynchronous or multiple-batch group-synchronous ovarian physiology. Based on the recent discovery of GnRH multiplicity in fish and the increasing understanding of its functional significance, new GnRH agonists can be designed for more potent, affordable and physiologically-compatible spawning induction therapies. Future strategies for improved spawning manipulations will be based on understanding the captivity-induced alterations in the GnRH system, and on new approaches for their repair at the level of GnRH gene expression and release. © 2001 Elsevier Science B.V. |
Mylonas, C C; Zohar, Y Endocrine regulation and artificial induction of oocyte maturation and spermiation in basses of the genus Morone Journal Article Aquaculture, 202 (3-4), pp. 205–220, 2001, ISSN: 00448486. @article{mylonas_endocrine_2001, title = {Endocrine regulation and artificial induction of oocyte maturation and spermiation in basses of the genus Morone}, author = {C C Mylonas and Y Zohar}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035510250&doi=10.1016%2fS0044-8486%2801%2900772-4&partnerID=40&md5=234515ff5965a8f17502acba9688f431}, doi = {10.1016/S0044-8486(01)00772-4}, issn = {00448486}, year = {2001}, date = {2001-01-01}, journal = {Aquaculture}, volume = {202}, number = {3-4}, pages = {205--220}, abstract = {Studying the process of final oocyte maturation (FOM) and spermiation in striped bass (Morone saxatilis), it was determined that the failure to undergo FOM and produce normal amounts of milt in captivity was due to low plasma levels of luteinizing hormone (LH) during the spawning period. As a result, the expected changes in the plasma profiles of the steroids involved in FOM and spermiation do not take place. Based on the hormone profiles and the associated histological examinations, FOM can be separated into an early phase (germinal vesicle (gv) migration) and a late phase (germinal vesicle breakdown). The progestogen 17,20β,21-trihydroxy-4-pregnen-3-one (17,20β,21-P) was identified as the maturation-inducing steroid (MIS), but the high levels of 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P) present during late FOM, suggest a role for this steroid also. In wild striped bass, spermiation was associated with increases in LH and unchanged plasma levels of 17,20β,21-P and 17, 20β-P. In culture, spermiation in both striped bass and white bass (M. chrysops) proceeds under undetectable levels of LH, 17,20β,21-P and 17,20β-P. GnRHa-induced spermiation, however, was associated with increases in plasma LH and 17,20β-P, but not 17,20β,21-P. Treatment of females with GnRHa-delivery systems induced long-term increases in plasma LH and the appropriate changes in sex-steroid hormones. In white bass, GnRHa-delivery systems induced two successive spawns within 3 days from treatment, and in a commercial production facility induced ovulation of 90% of the females, producing 294,500 eggs kg-1 with 81% fertilization. Similarly treated striped bass underwent FOM and ovulation within 10 days after treatment, while tank spawning resulted in the production of 170,000 eggs kg-1 with 47% fertilization success. In male fish, GnRHa-delivery systems induced significant elevations of expressible milt for up to 20 days, resulting in a fourfold increase in production of spermatozoa, without affecting sperm density, motility or fertilization %. © 2001 Published by Elsevier Science B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Studying the process of final oocyte maturation (FOM) and spermiation in striped bass (Morone saxatilis), it was determined that the failure to undergo FOM and produce normal amounts of milt in captivity was due to low plasma levels of luteinizing hormone (LH) during the spawning period. As a result, the expected changes in the plasma profiles of the steroids involved in FOM and spermiation do not take place. Based on the hormone profiles and the associated histological examinations, FOM can be separated into an early phase (germinal vesicle (gv) migration) and a late phase (germinal vesicle breakdown). The progestogen 17,20β,21-trihydroxy-4-pregnen-3-one (17,20β,21-P) was identified as the maturation-inducing steroid (MIS), but the high levels of 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P) present during late FOM, suggest a role for this steroid also. In wild striped bass, spermiation was associated with increases in LH and unchanged plasma levels of 17,20β,21-P and 17, 20β-P. In culture, spermiation in both striped bass and white bass (M. chrysops) proceeds under undetectable levels of LH, 17,20β,21-P and 17,20β-P. GnRHa-induced spermiation, however, was associated with increases in plasma LH and 17,20β-P, but not 17,20β,21-P. Treatment of females with GnRHa-delivery systems induced long-term increases in plasma LH and the appropriate changes in sex-steroid hormones. In white bass, GnRHa-delivery systems induced two successive spawns within 3 days from treatment, and in a commercial production facility induced ovulation of 90% of the females, producing 294,500 eggs kg-1 with 81% fertilization. Similarly treated striped bass underwent FOM and ovulation within 10 days after treatment, while tank spawning resulted in the production of 170,000 eggs kg-1 with 47% fertilization success. In male fish, GnRHa-delivery systems induced significant elevations of expressible milt for up to 20 days, resulting in a fourfold increase in production of spermatozoa, without affecting sperm density, motility or fertilization %. © 2001 Published by Elsevier Science B.V. |
Alasalvar, C; Taylor, K D A; Öksüz, A; Garthwaite, T; Alexis, M N; Grigorakis, K Freshness assessment of cultured sea bream (Sparus aurata) by chemical, physical and sensory methods Journal Article Food Chemistry, 72 (1), pp. 33–40, 2001, ISSN: 03088146. @article{alasalvar_freshness_2001, title = {Freshness assessment of cultured sea bream (Sparus aurata) by chemical, physical and sensory methods}, author = {C Alasalvar and K D A Taylor and A Öksüz and T Garthwaite and M N Alexis and K Grigorakis}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0308814600001965}, doi = {10.1016/S0308-8146(00)00196-5}, issn = {03088146}, year = {2001}, date = {2001-01-01}, urldate = {2020-08-10}, journal = {Food Chemistry}, volume = {72}, number = {1}, pages = {33--40}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Rigos, G; Pavlidis, M; Divanach, P Host susceptibility to Cryptocaryon sp. infection of Mediterranean marine broodfish held under intensive culture conditions: A case report Journal Article Bulletin of the European Association of Fish Pathologists, 21 , pp. 33 – 36, 2001. @article{rigos_host_2001, title = {Host susceptibility to Cryptocaryon sp. infection of Mediterranean marine broodfish held under intensive culture conditions: A case report}, author = {G Rigos and M Pavlidis and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0002831958&partnerID=40&md5=265b03fc4289569f541da14d6b89a6c8}, year = {2001}, date = {2001-01-01}, journal = {Bulletin of the European Association of Fish Pathologists}, volume = {21}, pages = {33 -- 36}, abstract = {The detrimental effect of Cryptocaryon irritans exhibiting a host specificity on several marine fish broodstock is described in this study. The parasite caused considerable mortalities on gilthead seabream, Sparus aurata, common dentex, Dentex dentex, and greater amberjack, Seriola dumerili, while red porgy, Pagrus pagrus, white sea bream, Diplodus sargus, sea bass, Dicentrarchus labrax, and sharpsnout sea bream, Diplodus puntazzo, remained unaffected. Chemical treatments were not found to be effective against the intense parasitosis and only extended, rapid and repeated hyposalinity measures seemed to minimise fish losses.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The detrimental effect of Cryptocaryon irritans exhibiting a host specificity on several marine fish broodstock is described in this study. The parasite caused considerable mortalities on gilthead seabream, Sparus aurata, common dentex, Dentex dentex, and greater amberjack, Seriola dumerili, while red porgy, Pagrus pagrus, white sea bream, Diplodus sargus, sea bass, Dicentrarchus labrax, and sharpsnout sea bream, Diplodus puntazzo, remained unaffected. Chemical treatments were not found to be effective against the intense parasitosis and only extended, rapid and repeated hyposalinity measures seemed to minimise fish losses. |
Panini, E B; Mylonas, C C; Zanuy, S; Carrillo, M; Ramos, J; Bruce, M P Incubation of embryos and larvae of marine fish using microtiter plates Journal Article Aquaculture International, 9 (2), pp. 189–195, 2001, ISSN: 09676120. @article{panini_incubation_2001, title = {Incubation of embryos and larvae of marine fish using microtiter plates}, author = {E B Panini and C C Mylonas and S Zanuy and M Carrillo and J Ramos and M P Bruce}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035574240&doi=10.1023%2fA%3a1014261830098&partnerID=40&md5=f38e791b9ea172d4e363686105d97e50}, doi = {10.1023/A:1014261830098}, issn = {09676120}, year = {2001}, date = {2001-01-01}, journal = {Aquaculture International}, volume = {9}, number = {2}, pages = {189--195}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Salvanes, AGV; Kristoffersen, JB Mesopelagic Fishes Incollection Steele, J; Thorpe, S; Turekian, K (Ed.): Encyclopedia of Ocean Sciences, pp. 1711–1717, Elsevier, 2001, ISBN: 978-0-12-227430-5. @incollection{salvanes_mesopelagic_2001, title = {Mesopelagic Fishes}, author = {AGV Salvanes and JB Kristoffersen}, editor = {J Steele and S Thorpe and K Turekian}, url = {http://www.sciencedirect.com/science/article/pii/B012227430X00012X}, doi = {10.1006/rwos.2001.0012}, isbn = {978-0-12-227430-5}, year = {2001}, date = {2001-01-01}, booktitle = {Encyclopedia of Ocean Sciences}, pages = {1711--1717}, publisher = {Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {incollection} } |
Cotou, E; Vagias, C; Rapti, T; Roussis, V Metallothionein levels in the bivalves Callista chione and Venus verrucosa from two mediterranean sites Journal Article Zeitschrift fur Naturforschung - Section C Journal of Biosciences, 56 , pp. 848 – 852, 2001. @article{cotou_metallothionein_2001, title = {Metallothionein levels in the bivalves Callista chione and Venus verrucosa from two mediterranean sites}, author = {E Cotou and C Vagias and T Rapti and V Roussis}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034761822&partnerID=40&md5=ff39bcb4ee40c3f1b5d1f4520eb3454d}, year = {2001}, date = {2001-01-01}, journal = {Zeitschrift fur Naturforschung - Section C Journal of Biosciences}, volume = {56}, pages = {848 -- 852}, abstract = {Metallothioneins levels (MTs) in the clams Callista chione and Venus verrucosa, collected from two coastal sites in Greece, were determined and quantified by SDS polyacrylamide gel electrophoresis (SDS-PAGE) and a spectrophotometric assay (Ellman’s reaction). SDS-PAGE separation in the digestive gland, which represents the hepato-pancreas in clams, demonstrated the presence of MTs similar to mammalian MT (rabbit liver Cd, Zn-thionein). No other SH-containing proteins apart from the MTs were detected. MT levels quantified by the Ellman’s reaction indicated seasonal variation for both species. The highest values were recorded in the spring and the lowest in the autumn. The seasonal variation and the differences in the MT levels of the two areas seem to be related to the reproductive cycle of the organisms as well as to abiotic factors of each area. Our results show that both C. chione and V. verrucosa have the potential to be used as biomarkers of metal pollution, provided that the influence of the external factors is safely quantified.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Metallothioneins levels (MTs) in the clams Callista chione and Venus verrucosa, collected from two coastal sites in Greece, were determined and quantified by SDS polyacrylamide gel electrophoresis (SDS-PAGE) and a spectrophotometric assay (Ellman’s reaction). SDS-PAGE separation in the digestive gland, which represents the hepato-pancreas in clams, demonstrated the presence of MTs similar to mammalian MT (rabbit liver Cd, Zn-thionein). No other SH-containing proteins apart from the MTs were detected. MT levels quantified by the Ellman’s reaction indicated seasonal variation for both species. The highest values were recorded in the spring and the lowest in the autumn. The seasonal variation and the differences in the MT levels of the two areas seem to be related to the reproductive cycle of the organisms as well as to abiotic factors of each area. Our results show that both C. chione and V. verrucosa have the potential to be used as biomarkers of metal pollution, provided that the influence of the external factors is safely quantified. |
Carrillo, J; Koumoundouros, G; Divanach, P; Martinez, J Morphological malformations of the lateral line in reared gilthead sea bream (Sparus aurata L. 1758) Journal Article Aquaculture, 192 (2-4), pp. 281–290, 2001, ISSN: 00448486. @article{carrillo_morphological_2001, title = {Morphological malformations of the lateral line in reared gilthead sea bream (Sparus aurata L. 1758)}, author = {J Carrillo and G Koumoundouros and P Divanach and J Martinez}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848600004543}, doi = {10.1016/S0044-8486(00)00454-3}, issn = {00448486}, year = {2001}, date = {2001-01-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {192}, number = {2-4}, pages = {281--290}, abstract = {A general description of the morphological modifications of the lateral line in gilthead sea bream, reared under hatchery conditions, is provided. Farm-reared fish were examined and compared to wild fish. We used the presence of a pore on the specialized scales of the lateral line to determine the density and distribution of associated sensory neuromasts. The malformations of the lateral line were: zigzag with missing sector, wavy with missing sector, several scattered missing sectors, and several consecutive missing sectors. These abnormalities occurred in 71.0-86.2% of the reared fish, depending on the hatchery. They did not occur in wild fish. The possible behaviour consequences for gilthead sea bream of deficient sensory reception are considered. In addition, the suitability of these abnormalities for use as individual markers in different types of studies is proposed. © 2001 Elsevier Science B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A general description of the morphological modifications of the lateral line in gilthead sea bream, reared under hatchery conditions, is provided. Farm-reared fish were examined and compared to wild fish. We used the presence of a pore on the specialized scales of the lateral line to determine the density and distribution of associated sensory neuromasts. The malformations of the lateral line were: zigzag with missing sector, wavy with missing sector, several scattered missing sectors, and several consecutive missing sectors. These abnormalities occurred in 71.0-86.2% of the reared fish, depending on the hatchery. They did not occur in wild fish. The possible behaviour consequences for gilthead sea bream of deficient sensory reception are considered. In addition, the suitability of these abnormalities for use as individual markers in different types of studies is proposed. © 2001 Elsevier Science B.V. |
Mandalakis, M; Tsapakis, M; Stephanou, E G Journal of Chromatography A, 925 (1-2), pp. 183–196, 2001, ISSN: 00219673, (Publisher: Elsevier). @article{mandalakis_optimization_2001, title = {Optimization and application of high-resolution gas chromatography with ion trap tandem mass spectrometry to the determination of polychlorinated biphenyls in atmospheric aerosols}, author = {M Mandalakis and M Tsapakis and E G Stephanou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035800692&doi=10.1016%2fS0021-9673%2801%2901033-0&partnerID=40&md5=bc2efef41df1a986a561509985d9f65b}, doi = {10.1016/S0021-9673(01)01033-0}, issn = {00219673}, year = {2001}, date = {2001-01-01}, journal = {Journal of Chromatography A}, volume = {925}, number = {1-2}, pages = {183--196}, abstract = {Optimization of the Finnigan GCQ ion trap mass spectrometry (ITMS) system and a clean-up procedure were carried out in order to apply high-resolution gas chromatography-tandem mass spectrometry for the analysis of polychlorinated biphenyls (PCBs) in aerosols. Six ITMS operating parameters, including isolation time, excitation voltage, excitation time, 'q' value, ion source temperature and electron energy were adjusted in order to optimize the instrument analytical performance. The adjustment of all parameters substantially increased the sensitivity of ITMS in the MS-MS mode. Changes in isolation time did not particularly affect ITMS sensitivity while ion source temperature had the strongest influence. After optimization, a limit of detection of 600 fg/μl with S/N varying from 8 up to 91 was achieved. The application of the optimized ITMS parameters conjointly with the developed clean-up procedure resulted in method detection limits of 10-20 fg/m3 for the determination of PCBs, in the particulate and gas phase of the atmospheric aerosol of background areas in the Eastern Mediterranean and Sweden. Copyright © 2001 Elsevier Science B.V.}, note = {Publisher: Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {article} } Optimization of the Finnigan GCQ ion trap mass spectrometry (ITMS) system and a clean-up procedure were carried out in order to apply high-resolution gas chromatography-tandem mass spectrometry for the analysis of polychlorinated biphenyls (PCBs) in aerosols. Six ITMS operating parameters, including isolation time, excitation voltage, excitation time, 'q' value, ion source temperature and electron energy were adjusted in order to optimize the instrument analytical performance. The adjustment of all parameters substantially increased the sensitivity of ITMS in the MS-MS mode. Changes in isolation time did not particularly affect ITMS sensitivity while ion source temperature had the strongest influence. After optimization, a limit of detection of 600 fg/μl with S/N varying from 8 up to 91 was achieved. The application of the optimized ITMS parameters conjointly with the developed clean-up procedure resulted in method detection limits of 10-20 fg/m3 for the determination of PCBs, in the particulate and gas phase of the atmospheric aerosol of background areas in the Eastern Mediterranean and Sweden. Copyright © 2001 Elsevier Science B.V. |
Greenwood, L N; Scott, A P; Vermeirssen, E L M; Mylonas, C C; Pavlidis, M Plasma steroids in mature common dentex (Dentex dentex) stimulated with a gonadotropin-releasing hormone agonist Journal Article General and Comparative Endocrinology, 123 (1), pp. 1–12, 2001, ISSN: 00166480. @article{greenwood_plasma_2001, title = {Plasma steroids in mature common dentex (Dentex dentex) stimulated with a gonadotropin-releasing hormone agonist}, author = {L N Greenwood and A P Scott and E L M Vermeirssen and C C Mylonas and M Pavlidis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034944955&doi=10.1006%2fgcen.2000.7519&partnerID=40&md5=a67970e64ad41e8b9ec71482ff619e51}, doi = {10.1006/gcen.2000.7519}, issn = {00166480}, year = {2001}, date = {2001-01-01}, journal = {General and Comparative Endocrinology}, volume = {123}, number = {1}, pages = {1--12}, abstract = {The aim of this study was to identify the major C21 steroids produced in vivo during artificially induced final oocyte maturation and spawning in female common dentex (Dentex dentex). During the spawning season, mature females were treated with a gonadotropin-releasing hormone agonist (GnRHa)-loaded delivery system, with or without pimozide (given as a single dose at the beginning of the experiment). Blood samples were collected at various intervals during the experiment and were assayed for GnRHa, 17, 20β-dihydroxy-4-pregnen-3-one (17, 20β-P), and 17, 20β, 21-trihydroxy-4-pregnen-3-one (17, 20β, 21-P). A higher percentage of ovulated females was observed in GnRHa-implanted fish, which produced over 10 times more eggs than controls. Relative fecundity was highest in the GnRHa + pimozide group and lowest in controls. The viability of naturally released eggs was low (2 to 15%) in all groups. Plasma concentrations of 17, 20β-P in GnRHa-implanted fish did not increase, but those in control fish decreased, such that there was a significant difference between control and treated fish between 2 and 10 days after treatment. In another experiment, ovulating common dentex were injected intramuscularly with a single dose of 50 μg kg−1 of GnRHa in saline and were sampled for blood at 0, 3, 6, 12, and 24 h postinjection. A single water sample was taken from the tanks at 9 h postinjection, the tanks having been emptied and refilled at 6 h. Measurements were made of plasma and water concentrations of free and conjugated 17, 20β-P, 17, 20β, 21-P, 17β-oestradiol (E2), and GnRHa (plasma only). The GnRHa injection increased plasma levels of all steroids, with free 17, 20β-P reaching maximal levels within 3 h. GnRHa treatment also increased the amounts of free and conjugated steroids released into the water between 6 and 9 h. © 2001 British Crown.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The aim of this study was to identify the major C21 steroids produced in vivo during artificially induced final oocyte maturation and spawning in female common dentex (Dentex dentex). During the spawning season, mature females were treated with a gonadotropin-releasing hormone agonist (GnRHa)-loaded delivery system, with or without pimozide (given as a single dose at the beginning of the experiment). Blood samples were collected at various intervals during the experiment and were assayed for GnRHa, 17, 20β-dihydroxy-4-pregnen-3-one (17, 20β-P), and 17, 20β, 21-trihydroxy-4-pregnen-3-one (17, 20β, 21-P). A higher percentage of ovulated females was observed in GnRHa-implanted fish, which produced over 10 times more eggs than controls. Relative fecundity was highest in the GnRHa + pimozide group and lowest in controls. The viability of naturally released eggs was low (2 to 15%) in all groups. Plasma concentrations of 17, 20β-P in GnRHa-implanted fish did not increase, but those in control fish decreased, such that there was a significant difference between control and treated fish between 2 and 10 days after treatment. In another experiment, ovulating common dentex were injected intramuscularly with a single dose of 50 μg kg−1 of GnRHa in saline and were sampled for blood at 0, 3, 6, 12, and 24 h postinjection. A single water sample was taken from the tanks at 9 h postinjection, the tanks having been emptied and refilled at 6 h. Measurements were made of plasma and water concentrations of free and conjugated 17, 20β-P, 17, 20β, 21-P, 17β-oestradiol (E2), and GnRHa (plasma only). The GnRHa injection increased plasma levels of all steroids, with free 17, 20β-P reaching maximal levels within 3 h. GnRHa treatment also increased the amounts of free and conjugated steroids released into the water between 6 and 9 h. © 2001 British Crown. |
Kristoffersen, J B; Salvanes, A G V Sexual size dimorphism and sex ratio in Müller's pearlside (Maurolicus muelleri) Journal Article Marine Biology, 138 (6), pp. 1087–1092, 2001, ISSN: 00253162. @article{kristoffersen_sexual_2001, title = {Sexual size dimorphism and sex ratio in Müller's pearlside (Maurolicus muelleri)}, author = {J B Kristoffersen and A G V Salvanes}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034933210&doi=10.1007%2Fs002270000529&partnerID=40&md5=231a7d359b6ca8692eeba623e13d94a0}, doi = {10.1007/s002270000529}, issn = {00253162}, year = {2001}, date = {2001-01-01}, journal = {Marine Biology}, volume = {138}, number = {6}, pages = {1087--1092}, abstract = {From a series of samples from the Norwegian Sea and fjords, we demonstrate sexual size dimorphism in Maurolicus muelleri, and geographical variation in the degree of dimorphism. The dimorphism was smaller in the Norwegian Sea than in the fjord populations. In fjords, males had a lower maximum size and higher mortality than females. One-year-old females weighed on average about 50% more than males in fjords, but this weight difference was reduced to about 10% among 3-year-old fish. Possible causes of this decreasing sexual size dimorphism are discussed.}, keywords = {}, pubstate = {published}, tppubtype = {article} } From a series of samples from the Norwegian Sea and fjords, we demonstrate sexual size dimorphism in Maurolicus muelleri, and geographical variation in the degree of dimorphism. The dimorphism was smaller in the Norwegian Sea than in the fjord populations. In fjords, males had a lower maximum size and higher mortality than females. One-year-old females weighed on average about 50% more than males in fjords, but this weight difference was reduced to about 10% among 3-year-old fish. Possible causes of this decreasing sexual size dimorphism are discussed. |
Forniés, M A; Mañanós, E; Carrillo, M; Rocha, A; Laureau, S; Mylonas, C C; Zohar, Y; Zanuy, S Spawning induction of individual European sea bass females (Dicentrarchus labrax) using different GnRHa-delivery systems Journal Article Aquaculture, 202 (3-4), pp. 221–234, 2001, ISSN: 00448486, (Publisher: Elsevier). @article{fornies_spawning_2001, title = {Spawning induction of individual European sea bass females (Dicentrarchus labrax) using different GnRHa-delivery systems}, author = {M A Forniés and E Mañanós and M Carrillo and A Rocha and S Laureau and C C Mylonas and Y Zohar and S Zanuy}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035509922&doi=10.1016%2fS0044-8486%2801%2900773-6&partnerID=40&md5=9b233b084d0915f643c35513f1180854}, doi = {10.1016/S0044-8486(01)00773-6}, issn = {00448486}, year = {2001}, date = {2001-01-01}, journal = {Aquaculture}, volume = {202}, number = {3-4}, pages = {221--234}, abstract = {The present study evaluated the effect of different delivery systems loaded with gonadotropin-releasing hormone agonist (GnRHa) on spawning induction, and egg and larval quality in female European sea bass (Dicentrarchus labrax). Mature females (mean ± S.E.M. oocyte diameter = 905 ± 10 μm) were treated with [D-Ala6, Pro9 NEt]-mGnRHa using GnRHa-loaded microspheres, Mc (60 μg kg-1 body weight, bw) GnRHa-loaded implants, EVAc (50 μg kg-1 bw) or received a single injection of GnRHa in saline, IN (15 μg kg-1 bw). Controls (C) were injected with saline. Females were allocated individually in 2-m3 tanks with two GnRHa-treated males and were allowed to spawn without further handling. All GnRHa treatments induced spawning 3 days after treatment (d.a.t.), whereas control fish did not spawn during the 21-day experimental period. GnRHa microspheres and implants induced up to four consecutive spawns in one female, whereas GnRHa-injected fish spawned only once. Egg quality seemed relatively lower in all GnRHa-treated groups compared to spontaneously spawning fish (natural spawns, NAT group) maintained in large populations (80 fish in 15-m3 tanks), possibly due to both the isolation of individual females in separate tanks and the stress from handling. Mean egg quality of the first spawns was variable, but similar in all GnRHa treatments (6.6-18.2% buoyancy; 21.7-27.4% hatching). In subsequent spawns, the Mc-treated females produced eggs of higher buoyancy than the EVAc group (20.5 ± 6.0% vs. 2.87 ± 1.14%), and similar to the first spawn of the GnRHa-injected group. Our results indicate that, compared to a single GnRHa injection, GnRHa-loaded microspheres can induce multiple spawns in European sea bass, without a negative effect on egg quality. © 2001 Elsevier Science B.V. All rights reserved.}, note = {Publisher: Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {article} } The present study evaluated the effect of different delivery systems loaded with gonadotropin-releasing hormone agonist (GnRHa) on spawning induction, and egg and larval quality in female European sea bass (Dicentrarchus labrax). Mature females (mean ± S.E.M. oocyte diameter = 905 ± 10 μm) were treated with [D-Ala6, Pro9 NEt]-mGnRHa using GnRHa-loaded microspheres, Mc (60 μg kg-1 body weight, bw) GnRHa-loaded implants, EVAc (50 μg kg-1 bw) or received a single injection of GnRHa in saline, IN (15 μg kg-1 bw). Controls (C) were injected with saline. Females were allocated individually in 2-m3 tanks with two GnRHa-treated males and were allowed to spawn without further handling. All GnRHa treatments induced spawning 3 days after treatment (d.a.t.), whereas control fish did not spawn during the 21-day experimental period. GnRHa microspheres and implants induced up to four consecutive spawns in one female, whereas GnRHa-injected fish spawned only once. Egg quality seemed relatively lower in all GnRHa-treated groups compared to spontaneously spawning fish (natural spawns, NAT group) maintained in large populations (80 fish in 15-m3 tanks), possibly due to both the isolation of individual females in separate tanks and the stress from handling. Mean egg quality of the first spawns was variable, but similar in all GnRHa treatments (6.6-18.2% buoyancy; 21.7-27.4% hatching). In subsequent spawns, the Mc-treated females produced eggs of higher buoyancy than the EVAc group (20.5 ± 6.0% vs. 2.87 ± 1.14%), and similar to the first spawn of the GnRHa-injected group. Our results indicate that, compared to a single GnRHa injection, GnRHa-loaded microspheres can induce multiple spawns in European sea bass, without a negative effect on egg quality. © 2001 Elsevier Science B.V. All rights reserved. |
Wilkes, D; Xie, S Q; Stickland, N C; Alami-Durante, H; Kentouri, M; Sterioti, A; Koumoundouros, G; Fauconneau, B; Goldspink, G Journal of Experimental Biology, 204 , pp. 2763 – 2771, 2001. @article{wilkes_temperature_2001, title = {Temperature and myogenic factor transcript levels during early development determines muscle growth potential in rainbow trout (Oncorhynchus mykiss) and sea bass (Dicentrarchus labrax)}, author = {D Wilkes and S Q Xie and N C Stickland and H Alami-Durante and M Kentouri and A Sterioti and G Koumoundouros and B Fauconneau and G Goldspink}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035745854&partnerID=40&md5=fd20f80568bada767740f83db9f355ac}, year = {2001}, date = {2001-01-01}, journal = {Journal of Experimental Biology}, volume = {204}, pages = {2763 -- 2771}, abstract = {The influence of changes in environmental temperature on the mRNA levels of myogenic regulatory factors (MRFs), i.e. MyoD and myogenin, as well as myosin heavy chain (MyHC) were studied during early larval development in rainbow trout and sea bass. Phosphoimager analysis of northern blots indicated that there is an optimum temperature for the RNA transcript levels of MRF and MyHC RNA in trout and in sea bass larvae. In the trout strain studied, the highest concentration for MRF and MyHC transcripts was found at 8°C rather than 4°C or 20°C. In European sea bass, the highest concentrations of MRF and MyHC mRNA were observed at 15-20°C rather than 13°C. Raising sea bass larvae at 15°C was associated with higher MyHC gene expression as well as a trend towards an increase in total muscle fibre number and higher growth rates after transfer at ambient temperature. Results suggest that mRNA levels of MRF and MyHC can be used to optimise early development. An experiment in which the temperature was changed illustrates the consequence of precise temporal expression of MRF genes in specifying muscle fibre number at critical stages during early development.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The influence of changes in environmental temperature on the mRNA levels of myogenic regulatory factors (MRFs), i.e. MyoD and myogenin, as well as myosin heavy chain (MyHC) were studied during early larval development in rainbow trout and sea bass. Phosphoimager analysis of northern blots indicated that there is an optimum temperature for the RNA transcript levels of MRF and MyHC RNA in trout and in sea bass larvae. In the trout strain studied, the highest concentration for MRF and MyHC transcripts was found at 8°C rather than 4°C or 20°C. In European sea bass, the highest concentrations of MRF and MyHC mRNA were observed at 15-20°C rather than 13°C. Raising sea bass larvae at 15°C was associated with higher MyHC gene expression as well as a trend towards an increase in total muscle fibre number and higher growth rates after transfer at ambient temperature. Results suggest that mRNA levels of MRF and MyHC can be used to optimise early development. An experiment in which the temperature was changed illustrates the consequence of precise temporal expression of MRF genes in specifying muscle fibre number at critical stages during early development. |
Antoniadou, Chryssanthi; Manoudis, G; Dounas, C; Kallianiotis, Argyris; Vidoris, P; Chintiroglou, Chariton Charles The colonization of experimental artificial reefs, from the serpulids polychaetes, in the N. Aegean Sea (Greece). preliminary results Inproceedings Rapp. Comm. Int. Mer Médit., pp. 352, Monaco, 2001. @inproceedings{antoniadou_colonization_2001, title = {The colonization of experimental artificial reefs, from the serpulids polychaetes, in the N. Aegean Sea (Greece). preliminary results}, author = {Chryssanthi Antoniadou and G Manoudis and C Dounas and Argyris Kallianiotis and P Vidoris and Chariton Charles Chintiroglou}, year = {2001}, date = {2001-01-01}, booktitle = {Rapp. Comm. Int. Mer Médit.}, volume = {36}, pages = {352}, address = {Monaco}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Rigos, G; Stevenson, R Aquaculture International, 9 , pp. 247 – 253, 2001. @article{rigos_effect_2001, title = {The effect of antibiotic treatment on the establishment of persistent infection with Yersinia ruckeri Serovar II in rainbow trout Oncorhynchus mykiss (Walbaum)}, author = {G Rigos and R Stevenson}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035741198&partnerID=40&md5=70a523c4134f5aee8b8488ffc10cc738}, year = {2001}, date = {2001-01-01}, journal = {Aquaculture International}, volume = {9}, pages = {247 -- 253}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Pollani, A; Triantafyllou, G; Petihakis, George; Nittis, K; Dounas, C; Koutitas, C The Poseidon operational tool for the prediction of floating pollutant transport Journal Article Marine Pollution Bulletin, 43 , pp. 270–278, 2001. @article{pollani_poseidon_2001, title = {The Poseidon operational tool for the prediction of floating pollutant transport}, author = {A Pollani and G Triantafyllou and George Petihakis and K Nittis and C Dounas and C Koutitas}, year = {2001}, date = {2001-01-01}, journal = {Marine Pollution Bulletin}, volume = {43}, pages = {270--278}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Papandroulakis, N; Divanach, P; Anastasiadis, P; Kentouri, M The pseudo-green water technique for intensive rearing of sea bream (Sparus aurata) larvae Journal Article Aquaculture International, 9 , pp. 205 – 216, 2001. @article{papandroulakis_pseudo-green_2001, title = {The pseudo-green water technique for intensive rearing of sea bream (Sparus aurata) larvae}, author = {N Papandroulakis and P Divanach and P Anastasiadis and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035741183&partnerID=40&md5=25026d07e6e77837a60e0648627158db}, year = {2001}, date = {2001-01-01}, journal = {Aquaculture International}, volume = {9}, pages = {205 -- 216}, abstract = {The "pseudo green water" technology for sea bream (Sparus aurata) larval rearing was evaluated by analyzing results of a 2-year study, performed in a pilot scale system. The technology is characterized by the daily addition of phytoplankton in the rearing tanks during the first month of rearing. Effects of egg origin, spawning season and initial larval density on the larval rearing were investigated. Fish reared in "pseudo green water" systems for 60 days, presented high biological performance in terms of survival (56 ± 16%), mean weight (62 ± 12 mg), total length (20 ± 1 mm), conformity with wild standard (88 ± 9%), and functional swim bladder (93 ± 4%). The results present homogenity between the categories (origin of eggs, time of spawning, initial egg density) studied, proving the stability of the technology.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The "pseudo green water" technology for sea bream (Sparus aurata) larval rearing was evaluated by analyzing results of a 2-year study, performed in a pilot scale system. The technology is characterized by the daily addition of phytoplankton in the rearing tanks during the first month of rearing. Effects of egg origin, spawning season and initial larval density on the larval rearing were investigated. Fish reared in "pseudo green water" systems for 60 days, presented high biological performance in terms of survival (56 ± 16%), mean weight (62 ± 12 mg), total length (20 ± 1 mm), conformity with wild standard (88 ± 9%), and functional swim bladder (93 ± 4%). The results present homogenity between the categories (origin of eggs, time of spawning, initial egg density) studied, proving the stability of the technology. |
Katharios, P; Iliopoulou-Georgudaki, J; Kapata-Zoumbos, K; Spiropoulos, S Toxicity of intraperitoneally injected ivermectin in sea bream, Sparus aurata Journal Article Fish Physiology and Biochemistry, 25 (2), pp. 99–108, 2001, ISSN: 09201742. @article{katharios_toxicity_2001, title = {Toxicity of intraperitoneally injected ivermectin in sea bream, Sparus aurata}, author = {P Katharios and J Iliopoulou-Georgudaki and K Kapata-Zoumbos and S Spiropoulos}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-14244271014&doi=10.1023%2fA%3a1020574810332&partnerID=40&md5=07b57b30739180b79477443f43f5a1e5}, doi = {10.1023/A:1020574810332}, issn = {09201742}, year = {2001}, date = {2001-01-01}, journal = {Fish Physiology and Biochemistry}, volume = {25}, number = {2}, pages = {99--108}, abstract = {Ivermectin has been widely used as a treatment against sea lice infections of farmed fish (salmonids and non-salmonids) without any official license for use in aquaculture. This study presents data on the toxic side effects of ivermectin administered intraperitoneally in sea bream, Sparus aurata at single doses of 100, 200, 400 and 800 μg ivermectin kg-1 fish. The toxicity of ivermectin was evaluated for a period of 35 days post treatment by determining changes in selected hematological parameters (hematocrit, hemoglobin, red blood cell count, leucocyte count, differential white blood cell count, plasma glucose, electrolytes, urea and alkaline phosphatase), pathological effects in the tissues and the organs of the fish using histological techniques and changes in the behavior of the fish. The results showed a decrease of the hematocrit value one hour after the injection for most of the doses along with an increase of the hemoglobin concentration, the number of the white blood cells, the lymphocytes and monocytes and plasma glucose. No mortality or any histopathological alteration at the tissues examined, were recorded. The fish, which were injected with the highest dose of ivermectin, experienced a loss of appetite, lethargy and darkening of their color during the first week post injection. All the studied parameters reached the normal level by the end of the experiment indicating a low and reversible toxicity of the drug.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Ivermectin has been widely used as a treatment against sea lice infections of farmed fish (salmonids and non-salmonids) without any official license for use in aquaculture. This study presents data on the toxic side effects of ivermectin administered intraperitoneally in sea bream, Sparus aurata at single doses of 100, 200, 400 and 800 μg ivermectin kg-1 fish. The toxicity of ivermectin was evaluated for a period of 35 days post treatment by determining changes in selected hematological parameters (hematocrit, hemoglobin, red blood cell count, leucocyte count, differential white blood cell count, plasma glucose, electrolytes, urea and alkaline phosphatase), pathological effects in the tissues and the organs of the fish using histological techniques and changes in the behavior of the fish. The results showed a decrease of the hematocrit value one hour after the injection for most of the doses along with an increase of the hemoglobin concentration, the number of the white blood cells, the lymphocytes and monocytes and plasma glucose. No mortality or any histopathological alteration at the tissues examined, were recorded. The fish, which were injected with the highest dose of ivermectin, experienced a loss of appetite, lethargy and darkening of their color during the first week post injection. All the studied parameters reached the normal level by the end of the experiment indicating a low and reversible toxicity of the drug. |
Bergh, Ø; Vikanes, L; Makridis, P; Skjermo, J; Knappskog, D; Rødseth, O M Uptake and processing of a Vibrio anguillarum bacterin in Artemia franciscana measured by ELISA and immunohistochemistry Journal Article Fish and Shellfish Immunology, 11 , pp. 15 – 22, 2001. @article{bergh_uptake_2001, title = {Uptake and processing of a Vibrio anguillarum bacterin in Artemia franciscana measured by ELISA and immunohistochemistry}, author = {Ø Bergh and L Vikanes and P Makridis and J Skjermo and D Knappskog and O M Rødseth}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035221650&partnerID=40&md5=00a741f967e4ad5935deb0eccaf166a6}, year = {2001}, date = {2001-01-01}, journal = {Fish and Shellfish Immunology}, volume = {11}, pages = {15 -- 22}, abstract = {Nauplii of Artemia franciscana were incubated in two different concentrations (undiluted and 1:9 in autoclaved sea water) of a divalent bacterin composed of two different serovars of Vibrio anguillarum. In order to investigate uptake and further processing of a bacterin in the live feed organism A. franciscana, immunohistochemistry was applied, visualising the presence of whole bacterial cells and antigens from the bacterin in individual nauplii. By using ELISA, it was shown that approximately 1·5-2·5×105cells were incorporated into each Artemia under the conditions used. Maximum incorporation of cells was measured after 30min, whereas after 60min there was a decline to levels of 0·9-1·6×105cells per Artemia. Immediately after incubation in the bacterin solution, the nauplii were transferred to a culture of the alga Isochrysis galbana, in order to simulate transfer of the nauplii to rearing tanks for fish larvae. From the ELISA, it could be concluded that the incorporated bacterial cells were excreted from the Artemia nauplii rapidly, however a large variation among different nauplii could be visualised by immunohistochemistry. © 2001 Academic Press.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Nauplii of Artemia franciscana were incubated in two different concentrations (undiluted and 1:9 in autoclaved sea water) of a divalent bacterin composed of two different serovars of Vibrio anguillarum. In order to investigate uptake and further processing of a bacterin in the live feed organism A. franciscana, immunohistochemistry was applied, visualising the presence of whole bacterial cells and antigens from the bacterin in individual nauplii. By using ELISA, it was shown that approximately 1·5-2·5×105cells were incorporated into each Artemia under the conditions used. Maximum incorporation of cells was measured after 30min, whereas after 60min there was a decline to levels of 0·9-1·6×105cells per Artemia. Immediately after incubation in the bacterin solution, the nauplii were transferred to a culture of the alga Isochrysis galbana, in order to simulate transfer of the nauplii to rearing tanks for fish larvae. From the ELISA, it could be concluded that the incorporated bacterial cells were excreted from the Artemia nauplii rapidly, however a large variation among different nauplii could be visualised by immunohistochemistry. © 2001 Academic Press. |
2000 |
Ali, Ben Z; Boursot, P; Said, K; Lagnel, J; Chatti, N; Navajas, M Comparison of Ribosomal ITS Regions Among Androctonus spp. Scorpions (Scorpionida: Buthidae) from Tunisia Journal Article Journal of Medical Entomology, 37 (6), pp. 787–790, 2000, ISSN: 0022-2585, 1938-2928. @article{ben_ali_comparison_2000, title = {Comparison of Ribosomal ITS Regions Among Androctonus spp. Scorpions (Scorpionida: Buthidae) from Tunisia}, author = {Z Ben Ali and P Boursot and K Said and J Lagnel and N Chatti and M Navajas}, url = {https://academic.oup.com/jme/article-lookup/doi/10.1603/0022-2585-37.6.787}, doi = {10.1603/0022-2585-37.6.787}, issn = {0022-2585, 1938-2928}, year = {2000}, date = {2000-11-01}, urldate = {2020-08-17}, journal = {Journal of Medical Entomology}, volume = {37}, number = {6}, pages = {787--790}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Karakassis, I Impact of cage farming of fish on the seabed in three Mediterranean coastal areas Journal Article ICES Journal of Marine Science, 57 (5), pp. 1462–1471, 2000, ISSN: 10543139. @article{karakassis_impact_2000, title = {Impact of cage farming of fish on the seabed in three Mediterranean coastal areas}, author = {I Karakassis}, url = {https://academic.oup.com/icesjms/article-lookup/doi/10.1006/jmsc.2000.0925}, doi = {10.1006/jmsc.2000.0925}, issn = {10543139}, year = {2000}, date = {2000-10-01}, urldate = {2020-08-18}, journal = {ICES Journal of Marine Science}, volume = {57}, number = {5}, pages = {1462--1471}, abstract = {The impact of cage culture of marine fish on the benthic environment was investigated seasonally at three commercial fish farms (Cephalonia, Ithaki, and Sounion) established in coastal waters 20-30 m deep, with different types of sediment (from 80% silt to coarse sand) and varying current intensity. A transect of stations in the vicinity of the cages and a control station in each area were sampled for macrofauna and geochemical variables during July, October, and April. Redox potential in the sediment decreased under and near the cages but reached negative values only at the silty sediment site. Organic carbon and nitrogen content of the sediment near the cages increased by a factor of 1.5-5 and ATP content by 4-28 compared with the control. Azoic zones were not encountered, but the macrofaunal community was affected up to 25 m from the edge of the cages. At the coarse sediment sites, abundance and biomass under the cages were 10 times higher than at the control. Diversity indicated that the ecotone was in the vicinity of 25 m from the cages in all cases. Capitella cf. capitata dominated macrofauna up to 10 m from the cages in two fish farms, whereas the third was dominated by Protodorvillea kefersteini. Similar patterns of succession from the impacted to the normal zones were found, although macrofaunal composition differed among sites. Seasonal variability in geochemistry and macrofauna was higher in proximity of the cages. The results indicate that impacts of fish farming on benthos in the Mediterranean vary considerably depending on site characteristics. © 2000 International Council for the Exploration of the Sea.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The impact of cage culture of marine fish on the benthic environment was investigated seasonally at three commercial fish farms (Cephalonia, Ithaki, and Sounion) established in coastal waters 20-30 m deep, with different types of sediment (from 80% silt to coarse sand) and varying current intensity. A transect of stations in the vicinity of the cages and a control station in each area were sampled for macrofauna and geochemical variables during July, October, and April. Redox potential in the sediment decreased under and near the cages but reached negative values only at the silty sediment site. Organic carbon and nitrogen content of the sediment near the cages increased by a factor of 1.5-5 and ATP content by 4-28 compared with the control. Azoic zones were not encountered, but the macrofaunal community was affected up to 25 m from the edge of the cages. At the coarse sediment sites, abundance and biomass under the cages were 10 times higher than at the control. Diversity indicated that the ecotone was in the vicinity of 25 m from the cages in all cases. Capitella cf. capitata dominated macrofauna up to 10 m from the cages in two fish farms, whereas the third was dominated by Protodorvillea kefersteini. Similar patterns of succession from the impacted to the normal zones were found, although macrofaunal composition differed among sites. Seasonal variability in geochemistry and macrofauna was higher in proximity of the cages. The results indicate that impacts of fish farming on benthos in the Mediterranean vary considerably depending on site characteristics. © 2000 International Council for the Exploration of the Sea. |
Henry, Morgane A; Secombes, Christopher J The A-layer influences the susceptibility of Aeromonas salmonicida to antibacterial peptides Journal Article Fish & Shellfish Immunology, 10 (7), pp. 637–642, 2000, ISSN: 10504648. @article{henry_-layer_2000, title = {The A-layer influences the susceptibility of Aeromonas salmonicida to antibacterial peptides}, author = {Morgane A Henry and Christopher J Secombes}, url = {https://linkinghub.elsevier.com/retrieve/pii/S1050464800902882}, doi = {10.1006/fsim.2000.0288}, issn = {10504648}, year = {2000}, date = {2000-10-01}, urldate = {2020-08-18}, journal = {Fish & Shellfish Immunology}, volume = {10}, number = {7}, pages = {637--642}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Tselepides, Anastasios; Papadopoulou, Konstantia-N; Podaras, Dimitris; Plaiti, Wanda; Koutsoubas, Drosos Macrobenthic community structure over the continental margin of Crete (South Aegean Sea, NE Mediterranean) Journal Article Progress in Oceanography, 46 (2-4), pp. 401–428, 2000, ISSN: 00796611. @article{tselepides_macrobenthic_2000, title = {Macrobenthic community structure over the continental margin of Crete (South Aegean Sea, NE Mediterranean)}, author = {Anastasios Tselepides and Konstantia-N Papadopoulou and Dimitris Podaras and Wanda Plaiti and Drosos Koutsoubas}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0079661100000276}, doi = {10.1016/S0079-6611(00)00027-6}, issn = {00796611}, year = {2000}, date = {2000-08-01}, urldate = {2020-08-11}, journal = {Progress in Oceanography}, volume = {46}, number = {2-4}, pages = {401--428}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Perrot-Minnot, Marie-Jeanne; Lagnel, Jacques; Migeon, Alain; Navajas, Maria Heredity, 84 (6), pp. 702–709, 2000, ISSN: 0018-067X, 1365-2540. @article{perrot-minnot_tracking_2000, title = {Tracking paternal genes with DALP markers in a pseudoarrhenotokous reproductive system: biparental transmission but haplodiploid-like inheritance in the mite Neoseiulus californicus}, author = {Marie-Jeanne Perrot-Minnot and Jacques Lagnel and Alain Migeon and Maria Navajas}, url = {http://link.springer.com/10.1046/j.1365-2540.2000.00708.x}, doi = {10.1046/j.1365-2540.2000.00708.x}, issn = {0018-067X, 1365-2540}, year = {2000}, date = {2000-06-01}, urldate = {2020-08-18}, journal = {Heredity}, volume = {84}, number = {6}, pages = {702--709}, abstract = {The complexity of some sexual reproductive systems in arthropods still leaves both their genetic and epigenetic determinism and their evolutionary significance poorly understood. Pseudoarrhenotoky is characterized by obligate fertilization and differential inactivation and/or elimination of paternal chromosomes in embryos that develop into males. Here, we investigate how the paternal genome is transmitted in a pseudoarrhenotokous mite, Neoseiulus californicus, using codominant genetic markers detected by DALP (direct amplification of length polymorphism). Transmission patterns of parental alleles through one and two generations are reported at four or five loci corresponding to four linkage groups. Our data provide strong evidence for selective elimination of the paternal genome among male tissues. Sperm contained maternal genes exclusively, whereas some male somatic tissues retained most if not all paternal chromosomes. No recombination between parental genomes prior to paternal genome elimination from the embryonic germ line was observed. These data allow a reinterpretation of previous phenotypic and cytogenetic observations in these mites, from which we suggest some relevant mechanistic and evolutionary implications. In addition, this is the first published study using polymorphic codominant loci detected by the recently developed DALP method.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The complexity of some sexual reproductive systems in arthropods still leaves both their genetic and epigenetic determinism and their evolutionary significance poorly understood. Pseudoarrhenotoky is characterized by obligate fertilization and differential inactivation and/or elimination of paternal chromosomes in embryos that develop into males. Here, we investigate how the paternal genome is transmitted in a pseudoarrhenotokous mite, Neoseiulus californicus, using codominant genetic markers detected by DALP (direct amplification of length polymorphism). Transmission patterns of parental alleles through one and two generations are reported at four or five loci corresponding to four linkage groups. Our data provide strong evidence for selective elimination of the paternal genome among male tissues. Sperm contained maternal genes exclusively, whereas some male somatic tissues retained most if not all paternal chromosomes. No recombination between parental genomes prior to paternal genome elimination from the embryonic germ line was observed. These data allow a reinterpretation of previous phenotypic and cytogenetic observations in these mites, from which we suggest some relevant mechanistic and evolutionary implications. In addition, this is the first published study using polymorphic codominant loci detected by the recently developed DALP method. |
Makridis, Pavlos; Fjellheim, Anders Jon; Skjermo, Jorunn; Vadstein, Olav Control of the bacterial flora of Brachionus plicatilis and Artemia franciscana by incubation in bacterial suspensions Journal Article Aquaculture, 185 (3-4), pp. 207–218, 2000, ISSN: 00448486. @article{makridis_control_2000, title = {Control of the bacterial flora of Brachionus plicatilis and Artemia franciscana by incubation in bacterial suspensions}, author = {Pavlos Makridis and Anders Jon Fjellheim and Jorunn Skjermo and Olav Vadstein}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848699003518}, doi = {10.1016/S0044-8486(99)00351-8}, issn = {00448486}, year = {2000}, date = {2000-05-01}, urldate = {2020-08-17}, journal = {Aquaculture}, volume = {185}, number = {3-4}, pages = {207--218}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Dounas, C; Arvanitidis, C; Koulouri, P Artificial reefs as a management tool for the delineation, protection and enhancement of coastal ecosystems Journal Article Fishing News, 228 , pp. 94–96 (In Greek), 2000. @article{dounas_artificial_2000, title = {Artificial reefs as a management tool for the delineation, protection and enhancement of coastal ecosystems}, author = {C Dounas and C Arvanitidis and P Koulouri}, year = {2000}, date = {2000-01-01}, journal = {Fishing News}, volume = {228}, pages = {94--96 (In Greek)}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Makridis, P; Fjellheim, Jon A; Skjermo, J; Vadstein, O Colonization of the gut in first feeding turbot by bacterial strains added to the water or bioencapsulated in rotifers Journal Article Aquaculture International, 8 , pp. 367 – 380, 2000. @article{makridis_colonization_2000, title = {Colonization of the gut in first feeding turbot by bacterial strains added to the water or bioencapsulated in rotifers}, author = {P Makridis and A Jon Fjellheim and J Skjermo and O Vadstein}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034526864&partnerID=40&md5=dd669b9377094049f452d8b00dac0ab5}, year = {2000}, date = {2000-01-01}, journal = {Aquaculture International}, volume = {8}, pages = {367 -- 380}, abstract = {Two bacterial strains, 4:44 and PB52, isolated from turbot (Scophthalmus maximus L.) were used during a first feeding experiment with turbot larvae. Bacteria were either added directly to the water on the day of hatching of the larvae (day 0), or bioencapsulated in rotifers (Brachionus plicarilis) distributed on day 2 after hatching. The two bacterial strains were found to be present in the water of the rearing tanks throughout the experiment. The addition of bacteria influenced the species-composition of the microflora associated with intensively produced rotifers added to the tanks, and resulted in colonization of the gut of the larvae by the added bacterial strains. The strain 4:44 showed a peak on day 9 after hatching, and reached a mean(sem) of 2.5(1.4) x 10 4 bacteria per larva, when added in a mixture with PB52. and 0.4(0.1) x 10 4 bacteria per larva, when added alone. The strain PB52, on day 12 after hatching, reached 5.2(1.5) x 10 4 bacteria per larva when added in a mixture with strain 4:44, and 12.5(0.7) x 10 4 bacteria per larva, when added alone. The added bacteria colonized the gut turbot larvae, whereas the bacterial land, the survival and growth of the larvae were in most cases not influenced in a negative way by the addition of bacteria.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Two bacterial strains, 4:44 and PB52, isolated from turbot (Scophthalmus maximus L.) were used during a first feeding experiment with turbot larvae. Bacteria were either added directly to the water on the day of hatching of the larvae (day 0), or bioencapsulated in rotifers (Brachionus plicarilis) distributed on day 2 after hatching. The two bacterial strains were found to be present in the water of the rearing tanks throughout the experiment. The addition of bacteria influenced the species-composition of the microflora associated with intensively produced rotifers added to the tanks, and resulted in colonization of the gut of the larvae by the added bacterial strains. The strain 4:44 showed a peak on day 9 after hatching, and reached a mean(sem) of 2.5(1.4) x 10 4 bacteria per larva, when added in a mixture with PB52. and 0.4(0.1) x 10 4 bacteria per larva, when added alone. The strain PB52, on day 12 after hatching, reached 5.2(1.5) x 10 4 bacteria per larva when added in a mixture with strain 4:44, and 12.5(0.7) x 10 4 bacteria per larva, when added alone. The added bacteria colonized the gut turbot larvae, whereas the bacterial land, the survival and growth of the larvae were in most cases not influenced in a negative way by the addition of bacteria. |
Koutsoubas, D; Arvanitidis, C; Dounas, C; Drummond, L Community structure and dynamics of the Molluscan Fauna in a Mediterranean lagoon (Gialova lagoon, SW Greece) Journal Article Belgian Journal of Zoology, 130 (SUPPL. 1), pp. 131–138, 2000, ISSN: 07776276. @article{koutsoubas_community_2000, title = {Community structure and dynamics of the Molluscan Fauna in a Mediterranean lagoon (Gialova lagoon, SW Greece)}, author = {D Koutsoubas and C Arvanitidis and C Dounas and L Drummond}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-78649411533&partnerID=40&md5=0db45fbd7fa9f9b765b6564d3c978855}, issn = {07776276}, year = {2000}, date = {2000-01-01}, journal = {Belgian Journal of Zoology}, volume = {130}, number = {SUPPL. 1}, pages = {131--138}, abstract = {The molluscan community of a shallow Mediterranean lagoon (Gialova lagoon, SW Greece, Ionian Sea) was studied on a seasonal basis during 1995-1996. A total of 23 species were recorded from the lagoon. Two of them namely: Placida viridis and Polycerella emertoni are reported for the first time from the Greek seas and the Eastern Mediterranean respectively. The dominant species were the gastropods Bittium reticulatum, Cerithium vulgatum, Pirenella conica, Hydrobia acuta, Cyclope neritea and the bivalves Cerastoderma glaucum, Abra ovata. Uni- and multivariate methods were employed to study the community structure and dynamics. On the basis of the multivariate pattern of the molluscan community a coenocline is observed which is strongly related with the degree of isolation. Two main zones with different faunal composition can be distinguished: one narrow zone close to the channel of communication with the sea and another in the innermost part of the lagoon. Following the confinement scale suggested for lagoonal ecosystems by GUELORGET & PERTHUISOT (1992) the first zone can be assigned to the "zone III" and the second to "zones IVV". The distribution pattern of the molluscan community is governed by a different set of environmental variables in each season, discussed in detail. Although no disturbance due to anthropogenic impact was revealed, this narrow lagoonal habitat was proved to suffer severe "dystrophic episodes", during late summer and the beginning of autumn, but successfully recovered, demonstrating a seasonal community pattern.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The molluscan community of a shallow Mediterranean lagoon (Gialova lagoon, SW Greece, Ionian Sea) was studied on a seasonal basis during 1995-1996. A total of 23 species were recorded from the lagoon. Two of them namely: Placida viridis and Polycerella emertoni are reported for the first time from the Greek seas and the Eastern Mediterranean respectively. The dominant species were the gastropods Bittium reticulatum, Cerithium vulgatum, Pirenella conica, Hydrobia acuta, Cyclope neritea and the bivalves Cerastoderma glaucum, Abra ovata. Uni- and multivariate methods were employed to study the community structure and dynamics. On the basis of the multivariate pattern of the molluscan community a coenocline is observed which is strongly related with the degree of isolation. Two main zones with different faunal composition can be distinguished: one narrow zone close to the channel of communication with the sea and another in the innermost part of the lagoon. Following the confinement scale suggested for lagoonal ecosystems by GUELORGET & PERTHUISOT (1992) the first zone can be assigned to the "zone III" and the second to "zones IVV". The distribution pattern of the molluscan community is governed by a different set of environmental variables in each season, discussed in detail. Although no disturbance due to anthropogenic impact was revealed, this narrow lagoonal habitat was proved to suffer severe "dystrophic episodes", during late summer and the beginning of autumn, but successfully recovered, demonstrating a seasonal community pattern. |
Magoulas, A; Kotoulas, G; Gérard, A; Naciri-Graven, Y; Dermitzakis, E; Hawkins, A J S Comparison of genetic variability and parentage in different ploidy classes of the Japanese oyster Crassostrea gigas Journal Article Genetical Research, 76 (3), pp. 261–272, 2000, ISSN: 00166723. @article{magoulas_comparison_2000, title = {Comparison of genetic variability and parentage in different ploidy classes of the Japanese oyster Crassostrea gigas}, author = {A Magoulas and G Kotoulas and A Gérard and Y Naciri-Graven and E Dermitzakis and A J S Hawkins}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034520835&doi=10.1017%2fS0016672300004778&partnerID=40&md5=6f9a177082cb2cf8620b0bf2ba31d383}, doi = {10.1017/S0016672300004778}, issn = {00166723}, year = {2000}, date = {2000-01-01}, journal = {Genetical Research}, volume = {76}, number = {3}, pages = {261--272}, abstract = {Chemical treatments with cytochalasin B were used to induce triploidy in the progeny of a mass fertilization of 3 male and 7 female Crassostrea gigas parents. Triploids were produced either by retention of the first (meiosis I (MI) triploids) or the second (meiosis II (MII) triploids) polar bodies. These animals, together with their diploid siblings, were divided for two experiments. One set was used to compare physiological performance, and the other set deployed to compare growth in two different natural environments. For both experiments, genetic variability in different ploidy classes was estimated using three microsatellite loci and eight allozyme loci. The microsatellite loci were highly polymorphic, allowing independent confirmation of ploidy status and the unambiguous identification of parentage for each oyster. Significant differences in parentage were found between ploidy classes, despite the fact they originated from the same mass fertilization. This indicates that the assumptions of a common genetic background among random samples of animals taken from the same mass fertilization may be generally valid. Knowledge of parentage also allowed the more accurate scoring of allozyme loci. As expected, triploids were found to be significantly more polymorphic than diploids. However, MI triploids were not significantly more polymorphic than MII triploids. MII triploid genotypes were used to estimate recombination rates between loci and their centromeres. These rates varied between 0.29 and 0.71, indicating only moderate chiasma interference.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Chemical treatments with cytochalasin B were used to induce triploidy in the progeny of a mass fertilization of 3 male and 7 female Crassostrea gigas parents. Triploids were produced either by retention of the first (meiosis I (MI) triploids) or the second (meiosis II (MII) triploids) polar bodies. These animals, together with their diploid siblings, were divided for two experiments. One set was used to compare physiological performance, and the other set deployed to compare growth in two different natural environments. For both experiments, genetic variability in different ploidy classes was estimated using three microsatellite loci and eight allozyme loci. The microsatellite loci were highly polymorphic, allowing independent confirmation of ploidy status and the unambiguous identification of parentage for each oyster. Significant differences in parentage were found between ploidy classes, despite the fact they originated from the same mass fertilization. This indicates that the assumptions of a common genetic background among random samples of animals taken from the same mass fertilization may be generally valid. Knowledge of parentage also allowed the more accurate scoring of allozyme loci. As expected, triploids were found to be significantly more polymorphic than diploids. However, MI triploids were not significantly more polymorphic than MII triploids. MII triploid genotypes were used to estimate recombination rates between loci and their centromeres. These rates varied between 0.29 and 0.71, indicating only moderate chiasma interference. |
Koukouras, A; Dounas, C Decapod crustaceans new to the fauna of the Aegean sea Journal Article Crustaceana, 73 (4), pp. 497–502, 2000, ISSN: 0011216X. @article{koukouras_decapod_2000, title = {Decapod crustaceans new to the fauna of the Aegean sea}, author = {A Koukouras and C Dounas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034487839&doi=10.1163%2f156854000504462&partnerID=40&md5=1bf21e323555907fd4a71a1a95c1fcb8}, doi = {10.1163/156854000504462}, issn = {0011216X}, year = {2000}, date = {2000-01-01}, journal = {Crustaceana}, volume = {73}, number = {4}, pages = {497--502}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Koumoundouros, G; Divanach, P; Kentouri, M Development of the skull in Dentex dentex (Osteichthyes: Sparidae) Journal Article Marine Biology, 136 (1), pp. 175–184, 2000, ISSN: 0025-3162, 1432-1793. @article{koumoundouros_development_2000, title = {Development of the skull in Dentex dentex (Osteichthyes: Sparidae)}, author = {G Koumoundouros and P Divanach and M Kentouri}, url = {http://link.springer.com/10.1007/s002270050020}, doi = {10.1007/s002270050020}, issn = {0025-3162, 1432-1793}, year = {2000}, date = {2000-01-01}, urldate = {2020-08-17}, journal = {Marine Biology}, volume = {136}, number = {1}, pages = {175--184}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Moschou, E A; Lasarte, Azpiroz U; Fouskaki, M; Chaniotakis, N A; Papandroulakis, N; Divanach, P Direct electrochemical flow analysis system for simultaneous monitoring of total ammonia and nitrite in seawater Journal Article Aquacultural Engineering, 22 , pp. 255 – 268, 2000. @article{moschou_direct_2000, title = {Direct electrochemical flow analysis system for simultaneous monitoring of total ammonia and nitrite in seawater}, author = {E A Moschou and U Azpiroz Lasarte and M Fouskaki and N A Chaniotakis and N Papandroulakis and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034128510&partnerID=40&md5=b82b0b1ee44766fb1f6d6e77b70ec3a9}, year = {2000}, date = {2000-01-01}, journal = {Aquacultural Engineering}, volume = {22}, pages = {255 -- 268}, abstract = {The design and the performance of a new portable flow analysis (FA) system for the continuous, fast and accurate measurement of total ammonia and nitrite content in non-filtered seawater samples is demonstrated. The complete system has been optimized to operate within the ammonia and nitrite concentration range of 0.05-10 ppm. The system offers good reproducibility (textless 5%) and stability (textless 0.02 ppm/h) at constant temperature, while the analysis time is in the order of 1.5-4 min depending on the sample analyzed. The analysis results of seawater samples obtained with the FA system were compared to that obtained with the standard colorimetric method and establish the suitability of the analyzer for the precise and continuous measurements of untreated samples for both in field and laboratory applications. In addition, its small size and weight offer the advantage of portability, while its datalogging capabilities also allow for independent ammonia and nitrite monitoring. (C) 2000 Elsevier Science B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The design and the performance of a new portable flow analysis (FA) system for the continuous, fast and accurate measurement of total ammonia and nitrite content in non-filtered seawater samples is demonstrated. The complete system has been optimized to operate within the ammonia and nitrite concentration range of 0.05-10 ppm. The system offers good reproducibility (textless 5%) and stability (textless 0.02 ppm/h) at constant temperature, while the analysis time is in the order of 1.5-4 min depending on the sample analyzed. The analysis results of seawater samples obtained with the FA system were compared to that obtained with the standard colorimetric method and establish the suitability of the analyzer for the precise and continuous measurements of untreated samples for both in field and laboratory applications. In addition, its small size and weight offer the advantage of portability, while its datalogging capabilities also allow for independent ammonia and nitrite monitoring. (C) 2000 Elsevier Science B.V. |
Publications
2002 |
The contribution of decapod crustaceans to the hyperbenthic fauna of the deep Mediterranean Sea. (poster) Inproceedings Corfu, Greece. 2-6 September., 2002, (Publication Title: The 8th Colloquium Crustacea Decapoda Mediterranea. Type: Poster). |
The Mediterranean, a living sea - A kit for environmental education and awareness for children Inproceedings Miami, Florida, 2002, (Publication Title: 22nd Annual Symposium on Sea Turtle Biology and Conservation Type: Poster). |
Turbulent free-surface flow over a sinusoidal bed Inproceedings pp. 241–246, 2002, (Publication Title: International Conference ‘Hydroinformatics-2002). |
Two new Barbus species from the Danube River basin, with redescription of B. petenyi (Teleostei: Cyprinidae) Journal Article Folia Zoologica, 51 (3), pp. 227–240, 2002. |
Validation of annuli on scales and otoliths of common dentex (Dentex dentex) Journal Article Fisheries Research, 54 (2), pp. 287–294, 2002, ISSN: 01657836. |
2001 |
Utilization of waste material resulting from trout processing in gilthead bream (Sparus aurata L.) diets Journal Article Aquaculture Research, 32 , pp. 288–295, 2001, ISSN: 1355557X, 13652109. |
Osteological development of the vertebral column and of the fins in Diplodus sargus (Teleostei: Perciformes: Sparidae) Journal Article Marine Biology, 139 (5), pp. 853 – 862, 2001, ISSN: 0025-3162, 1432-1793. |
Temperature-induced ontogenetic plasticity in sea bass (Dicentrarchus labrax) Journal Article Marine Biology, 139 (5), pp. 817 – 830, 2001, ISSN: 0025-3162, 1432-1793. |
Age and growth of Carassius auratus gibelio (Linnaeus, 1758) (Pisces: Cyprinidae) in Lake Lysimachia (W. Greece). Inproceedings of Ichthyologists, Panhellenic Society (Ed.): 10th Panhellenic Conference of Ichthyologists, pp. 257–259, Chania, Greece, 2001. |
The effect of rearing conditions on development of saddleback syndrome and caudal fin deformities in Dentex dentex (L.) Journal Article Aquaculture, 200 (3-4), pp. 285–304, 2001, ISSN: 00448486. |
Developmental stability and genetic heterozygosity in wild and cultured stocks of gilthead sea bream (Sparus aurata) Journal Article Journal of the Marine Biological Association of the United Kingdom, 81 (2), pp. 283–288, 2001, ISSN: 0025-3154, 1469-7769. |
Biological Control, 20 (3), pp. 191–198, 2001, ISSN: 10499644. |
Surfactant-based oil dispersant toxicity to developing nauplii of Artemia: Effects on ATPase enzymatic system Journal Article Chemosphere, 42 (8), pp. 959–964, 2001, ISSN: 00456535. |
Osteological development of Dentex dentex (Osteichthyes: Sparidae): dorsal, anal, paired fins and squamation Journal Article Marine Biology, 138 (2), pp. 399–406, 2001, ISSN: 0025-3162, 1432-1793. |
Addition of bacteria bioencapsulated in Artemia metanauplii to a rearing system for halibut larvae Journal Article Aquaculture International, 9 , pp. 225 – 235, 2001. |
Assessing marine ecosystem response to nutrient inputs Journal Article Marine Pollution Bulletin, 43 , pp. 175–186, 2001. |
Effects of different dietary oils in sea bass (Dicentrarchus labrax) nutrition Journal Article Aquaculture International, 9 , pp. 463 – 476, 2001. |
Effets biologiques de la pollution environmentale dans les ecosystemes marins cotiers Journal Article Journal de Recherche Océanographique, 26 (3), pp. 31–34, 2001. |
Endocrine manipulations of spawning in cultured fish: From hormones to genes Journal Article Aquaculture, 197 (1-4), pp. 99–136, 2001, ISSN: 00448486. |
Endocrine regulation and artificial induction of oocyte maturation and spermiation in basses of the genus Morone Journal Article Aquaculture, 202 (3-4), pp. 205–220, 2001, ISSN: 00448486. |
Freshness assessment of cultured sea bream (Sparus aurata) by chemical, physical and sensory methods Journal Article Food Chemistry, 72 (1), pp. 33–40, 2001, ISSN: 03088146. |
Host susceptibility to Cryptocaryon sp. infection of Mediterranean marine broodfish held under intensive culture conditions: A case report Journal Article Bulletin of the European Association of Fish Pathologists, 21 , pp. 33 – 36, 2001. |
Incubation of embryos and larvae of marine fish using microtiter plates Journal Article Aquaculture International, 9 (2), pp. 189–195, 2001, ISSN: 09676120. |
Mesopelagic Fishes Incollection Steele, J; Thorpe, S; Turekian, K (Ed.): Encyclopedia of Ocean Sciences, pp. 1711–1717, Elsevier, 2001, ISBN: 978-0-12-227430-5. |
Metallothionein levels in the bivalves Callista chione and Venus verrucosa from two mediterranean sites Journal Article Zeitschrift fur Naturforschung - Section C Journal of Biosciences, 56 , pp. 848 – 852, 2001. |
Morphological malformations of the lateral line in reared gilthead sea bream (Sparus aurata L. 1758) Journal Article Aquaculture, 192 (2-4), pp. 281–290, 2001, ISSN: 00448486. |
Journal of Chromatography A, 925 (1-2), pp. 183–196, 2001, ISSN: 00219673, (Publisher: Elsevier). |
Plasma steroids in mature common dentex (Dentex dentex) stimulated with a gonadotropin-releasing hormone agonist Journal Article General and Comparative Endocrinology, 123 (1), pp. 1–12, 2001, ISSN: 00166480. |
Sexual size dimorphism and sex ratio in Müller's pearlside (Maurolicus muelleri) Journal Article Marine Biology, 138 (6), pp. 1087–1092, 2001, ISSN: 00253162. |
Spawning induction of individual European sea bass females (Dicentrarchus labrax) using different GnRHa-delivery systems Journal Article Aquaculture, 202 (3-4), pp. 221–234, 2001, ISSN: 00448486, (Publisher: Elsevier). |
Journal of Experimental Biology, 204 , pp. 2763 – 2771, 2001. |
The colonization of experimental artificial reefs, from the serpulids polychaetes, in the N. Aegean Sea (Greece). preliminary results Inproceedings Rapp. Comm. Int. Mer Médit., pp. 352, Monaco, 2001. |
Aquaculture International, 9 , pp. 247 – 253, 2001. |
The Poseidon operational tool for the prediction of floating pollutant transport Journal Article Marine Pollution Bulletin, 43 , pp. 270–278, 2001. |
The pseudo-green water technique for intensive rearing of sea bream (Sparus aurata) larvae Journal Article Aquaculture International, 9 , pp. 205 – 216, 2001. |
Toxicity of intraperitoneally injected ivermectin in sea bream, Sparus aurata Journal Article Fish Physiology and Biochemistry, 25 (2), pp. 99–108, 2001, ISSN: 09201742. |
Uptake and processing of a Vibrio anguillarum bacterin in Artemia franciscana measured by ELISA and immunohistochemistry Journal Article Fish and Shellfish Immunology, 11 , pp. 15 – 22, 2001. |
2000 |
Comparison of Ribosomal ITS Regions Among Androctonus spp. Scorpions (Scorpionida: Buthidae) from Tunisia Journal Article Journal of Medical Entomology, 37 (6), pp. 787–790, 2000, ISSN: 0022-2585, 1938-2928. |
Impact of cage farming of fish on the seabed in three Mediterranean coastal areas Journal Article ICES Journal of Marine Science, 57 (5), pp. 1462–1471, 2000, ISSN: 10543139. |
The A-layer influences the susceptibility of Aeromonas salmonicida to antibacterial peptides Journal Article Fish & Shellfish Immunology, 10 (7), pp. 637–642, 2000, ISSN: 10504648. |
Macrobenthic community structure over the continental margin of Crete (South Aegean Sea, NE Mediterranean) Journal Article Progress in Oceanography, 46 (2-4), pp. 401–428, 2000, ISSN: 00796611. |
Heredity, 84 (6), pp. 702–709, 2000, ISSN: 0018-067X, 1365-2540. |
Control of the bacterial flora of Brachionus plicatilis and Artemia franciscana by incubation in bacterial suspensions Journal Article Aquaculture, 185 (3-4), pp. 207–218, 2000, ISSN: 00448486. |
Artificial reefs as a management tool for the delineation, protection and enhancement of coastal ecosystems Journal Article Fishing News, 228 , pp. 94–96 (In Greek), 2000. |
Colonization of the gut in first feeding turbot by bacterial strains added to the water or bioencapsulated in rotifers Journal Article Aquaculture International, 8 , pp. 367 – 380, 2000. |
Community structure and dynamics of the Molluscan Fauna in a Mediterranean lagoon (Gialova lagoon, SW Greece) Journal Article Belgian Journal of Zoology, 130 (SUPPL. 1), pp. 131–138, 2000, ISSN: 07776276. |
Comparison of genetic variability and parentage in different ploidy classes of the Japanese oyster Crassostrea gigas Journal Article Genetical Research, 76 (3), pp. 261–272, 2000, ISSN: 00166723. |
Decapod crustaceans new to the fauna of the Aegean sea Journal Article Crustaceana, 73 (4), pp. 497–502, 2000, ISSN: 0011216X. |
Development of the skull in Dentex dentex (Osteichthyes: Sparidae) Journal Article Marine Biology, 136 (1), pp. 175–184, 2000, ISSN: 0025-3162, 1432-1793. |
Direct electrochemical flow analysis system for simultaneous monitoring of total ammonia and nitrite in seawater Journal Article Aquacultural Engineering, 22 , pp. 255 – 268, 2000. |