2003 |
Poulakakis, N; Lymberakis, P; Antoniou, A; Chalkia, D; Zouros, E; Mylonas, M; Valakos, E Molecular phylogeny and biogeography of the wall-lizard Podarcis erhardii (Squamata: Lacertidae) Journal Article Molecular Phylogenetics and Evolution, 28 , pp. 38–46, 2003. @article{poulakakis_molecular_2003, title = {Molecular phylogeny and biogeography of the wall-lizard Podarcis erhardii (Squamata: Lacertidae)}, author = {N Poulakakis and P Lymberakis and A Antoniou and D Chalkia and E Zouros and M Mylonas and E Valakos}, url = {http://www.sciencedirect.com/science/article/pii/S105579030300037X}, doi = {DOI:.org/10.1016/S1055-7903(03)00037-X}, year = {2003}, date = {2003-01-01}, journal = {Molecular Phylogenetics and Evolution}, volume = {28}, pages = {38--46}, abstract = {Erhard's wall lizard, Podarcis erhardii (Sauria: Lacertidae), is highly diversified in Greece and especially in the southern Aegean region. Out of the 28 recognized subspecies, 27 are found in Greece from the North Sporades island-complex in the North Aegean (grossly south of the 39th parallel) to the island of Crete in the South. The species exhibits great morphological and ecological plasticity and inhabits many different habitats from rocky islets and sandy shores to mountaintops as high as 2000 m. By examining intraspecific variability at a segment of the mitochondrial gene cytochrome b we have found that that extant populations of P. erhardii are paraphyletic. Furthermore, we have found that subspecies previously defined on the basis of morphological characteristics do not correspond to different molecular phylogenetic clades, so that their status should be reconsidered. The DNA based biogeographical and phylogenetic history of Podarcis in Southern Greece is congruent with available paleogeographic data of the region, which supports the view that DNA sequences may be a useful tool for the study of palaeogeography.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Erhard's wall lizard, Podarcis erhardii (Sauria: Lacertidae), is highly diversified in Greece and especially in the southern Aegean region. Out of the 28 recognized subspecies, 27 are found in Greece from the North Sporades island-complex in the North Aegean (grossly south of the 39th parallel) to the island of Crete in the South. The species exhibits great morphological and ecological plasticity and inhabits many different habitats from rocky islets and sandy shores to mountaintops as high as 2000 m. By examining intraspecific variability at a segment of the mitochondrial gene cytochrome b we have found that that extant populations of P. erhardii are paraphyletic. Furthermore, we have found that subspecies previously defined on the basis of morphological characteristics do not correspond to different molecular phylogenetic clades, so that their status should be reconsidered. The DNA based biogeographical and phylogenetic history of Podarcis in Southern Greece is congruent with available paleogeographic data of the region, which supports the view that DNA sequences may be a useful tool for the study of palaeogeography. |
Mylonas, C C; Sigelaki, I; Divanach, P; Mananõs, E; Carrillo, M; Afonso-Polyviou, A Multiple spawning and egg quality of individual European sea bass (Dicentrarchus labrax) females after repeated injections of GnRHa Journal Article Aquaculture, 221 (1-4), pp. 605–620, 2003, ISSN: 00448486, (Publisher: Elsevier). @article{mylonas_multiple_2003, title = {Multiple spawning and egg quality of individual European sea bass (Dicentrarchus labrax) females after repeated injections of GnRHa}, author = {C C Mylonas and I Sigelaki and P Divanach and E Mananõs and M Carrillo and A Afonso-Polyviou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037402489&doi=10.1016%2fS0044-8486%2803%2900120-0&partnerID=40&md5=4fa3ca75d178e099a929643bd3f005cd}, doi = {10.1016/S0044-8486(03)00120-0}, issn = {00448486}, year = {2003}, date = {2003-01-01}, journal = {Aquaculture}, volume = {221}, number = {1-4}, pages = {605--620}, abstract = {Using multiple injections of gonadotropin-releasing hormone agonist (GnRHa) (10 μg kg-1 bw) spaced 7-14 days apart, we investigated the multiple-batch group-synchronous spawning kinetics of individual European sea bass females (n = 6) and examined batch fecundity and egg quality parameters. Spawning was obtained 3-4 days after each GnRHa injection. A mean of 2.8 spawns female-1 was obtained, with a minimum of 2 and maximum of 4. Mean relative fecundity of the first spawn was 320,211 eggs kg-1 bw and decreased continuously in subsequent spawns, reaching 52,381 eggs kg-1. Total mean relative fecundity for all spawns was 637,900 eggs kg-1. Mean fertilization success did not vary significantly among sequential spawns and ranged between 67% and 91%. On the contrary, there were significant differences among the four spawns in embryo survival 1 day after egg collection, hatching percentage and larval survival 4 days after egg collection, but there was no consistent trend during the study. Significant positive correlations existed between 4-day larval survival and hatching percentage (P=0.0002), and between hatching percentage and embryo survival 1 day after egg collection (P=0.03), indicating that 1-day embryo survival can be a potential biomarker for egg quality in commercial hatcheries. The results of the study demonstrate that European sea bass can produce up to four sequential spawns of high quality eggs in response to repeated acute treatments of GnRHa, and suggest that a pulsatile treatment of GnRHa may be necessary to induce the appropriate cycles of hormone changes for the recruitment, maturation and ovulation of multiple batches of viable eggs. © 2003 Elsevier Science B.V. All rights reserved.}, note = {Publisher: Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {article} } Using multiple injections of gonadotropin-releasing hormone agonist (GnRHa) (10 μg kg-1 bw) spaced 7-14 days apart, we investigated the multiple-batch group-synchronous spawning kinetics of individual European sea bass females (n = 6) and examined batch fecundity and egg quality parameters. Spawning was obtained 3-4 days after each GnRHa injection. A mean of 2.8 spawns female-1 was obtained, with a minimum of 2 and maximum of 4. Mean relative fecundity of the first spawn was 320,211 eggs kg-1 bw and decreased continuously in subsequent spawns, reaching 52,381 eggs kg-1. Total mean relative fecundity for all spawns was 637,900 eggs kg-1. Mean fertilization success did not vary significantly among sequential spawns and ranged between 67% and 91%. On the contrary, there were significant differences among the four spawns in embryo survival 1 day after egg collection, hatching percentage and larval survival 4 days after egg collection, but there was no consistent trend during the study. Significant positive correlations existed between 4-day larval survival and hatching percentage (P=0.0002), and between hatching percentage and embryo survival 1 day after egg collection (P=0.03), indicating that 1-day embryo survival can be a potential biomarker for egg quality in commercial hatcheries. The results of the study demonstrate that European sea bass can produce up to four sequential spawns of high quality eggs in response to repeated acute treatments of GnRHa, and suggest that a pulsatile treatment of GnRHa may be necessary to induce the appropriate cycles of hormone changes for the recruitment, maturation and ovulation of multiple batches of viable eggs. © 2003 Elsevier Science B.V. All rights reserved. |
Mandalakis, M; Gustafsson, Ö Journal of Chromatography A, 996 (1-2), pp. 163–172, 2003, ISSN: 00219673, (Publisher: Elsevier). @article{mandalakis_optimization_2003, title = {Optimization of a preparative capillary gas chromatography-mass spectrometry system for the isolation and harvesting of individual polycyclic aromatic hydrocarbons}, author = {M Mandalakis and Ö Gustafsson}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0038021269&doi=10.1016%2fS0021-9673%2803%2900612-5&partnerID=40&md5=e062f00b78a8f49e937856db9a1d8a87}, doi = {10.1016/S0021-9673(03)00612-5}, issn = {00219673}, year = {2003}, date = {2003-01-01}, journal = {Journal of Chromatography A}, volume = {996}, number = {1-2}, pages = {163--172}, abstract = {Operation parameters of a preparative capillary gas chromatography (pcGC) system were optimized to facilitate clean and efficient harvesting of individual polycyclic aromatic hydrocarbons (PAHs) for subsequent compound-specific radiocarbon analysis. For PAHs, the recommended optimized settings of the specially-designed pcGC cooled injection system (CIS) and preparative fraction collector (PFC) are: 5 s CIS solvent venting time, deactivation of CIS "stop flow" injection mode, autoinjector "fast injection" mode, 60 s CIS splitless time, 340°C PFC switch temperature, and 30°C (ambient) trapping temperature. These optimized conditions yielded highly reproducible, pure, and efficient pcGC harvesting of six PAHs with mass recoveries of 90-100% and purity of the isolates of 97-100%. © 2003 Elsevier Science B.V. All rights reserved.}, note = {Publisher: Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {article} } Operation parameters of a preparative capillary gas chromatography (pcGC) system were optimized to facilitate clean and efficient harvesting of individual polycyclic aromatic hydrocarbons (PAHs) for subsequent compound-specific radiocarbon analysis. For PAHs, the recommended optimized settings of the specially-designed pcGC cooled injection system (CIS) and preparative fraction collector (PFC) are: 5 s CIS solvent venting time, deactivation of CIS "stop flow" injection mode, autoinjector "fast injection" mode, 60 s CIS splitless time, 340°C PFC switch temperature, and 30°C (ambient) trapping temperature. These optimized conditions yielded highly reproducible, pure, and efficient pcGC harvesting of six PAHs with mass recoveries of 90-100% and purity of the isolates of 97-100%. © 2003 Elsevier Science B.V. All rights reserved. |
Rigos, G; Nengas, I; Tyrpenou, A E; Alexis, M; Troisi, G M Pharmacokinetics and bioavailability of oxytetracycline in gilthead sea bream (Sparus aurata) after a single dose Journal Article Aquaculture, 221 , pp. 75 – 83, 2003. @article{rigos_pharmacokinetics_2003, title = {Pharmacokinetics and bioavailability of oxytetracycline in gilthead sea bream (Sparus aurata) after a single dose}, author = {G Rigos and I Nengas and A E Tyrpenou and M Alexis and G M Troisi}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0037402546&partnerID=40&md5=73df0faf1da29455a07de80aa4cde170}, year = {2003}, date = {2003-01-01}, journal = {Aquaculture}, volume = {221}, pages = {75 -- 83}, abstract = {This is the first study to investigate the pharmacokinetic parameters of oxytetracycline (OTC) in gilthead sea bream (Sparus aurata). Following a single intravascular injection of OTC (40 mg/kg) in 100 g fish kept at 20°C, the distribution half-life (t1/2α) and the elimination half-life (t1/2β) of OTC were found to be long (2 and 53 h, respectively). The drug was well distributed throughout the internal body compartments since the apparent volume of distribution of the drug at steady-state (Vd(ss)) and the apparent volume of distribution by the area method (Vd(arca)) were found to be high (2.9 and 3.8 l/kg, respectively). OTC had a long mean residence time (MRT) (56 h) but a short total clearance (CLT) (0.05 1/kg h). The slow elimination of the drug suggests a need for long withdrawal periods prior to use of dosed fish for human consumption. Although the bioavailability (F%) of OTC following oral administration in aqueous suspension (75 mg/kg) was calculated to be low (9%), maximum OTC plasma concentration was relatively high (2.5 μg/ml), indicating that current therapeutic regimens applied to gilthead sea bream might be effective against bacterial pathogens. © 2003 Elsevier Science B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This is the first study to investigate the pharmacokinetic parameters of oxytetracycline (OTC) in gilthead sea bream (Sparus aurata). Following a single intravascular injection of OTC (40 mg/kg) in 100 g fish kept at 20°C, the distribution half-life (t1/2α) and the elimination half-life (t1/2β) of OTC were found to be long (2 and 53 h, respectively). The drug was well distributed throughout the internal body compartments since the apparent volume of distribution of the drug at steady-state (Vd(ss)) and the apparent volume of distribution by the area method (Vd(arca)) were found to be high (2.9 and 3.8 l/kg, respectively). OTC had a long mean residence time (MRT) (56 h) but a short total clearance (CLT) (0.05 1/kg h). The slow elimination of the drug suggests a need for long withdrawal periods prior to use of dosed fish for human consumption. Although the bioavailability (F%) of OTC following oral administration in aqueous suspension (75 mg/kg) was calculated to be low (9%), maximum OTC plasma concentration was relatively high (2.5 μg/ml), indicating that current therapeutic regimens applied to gilthead sea bream might be effective against bacterial pathogens. © 2003 Elsevier Science B.V. All rights reserved. |
Rigos, G; Tyrpenou, A E; Nengas, I; Yiagnisis, M; Koutsodimou, M; Alexis, M; Troisi, G M Pharmacokinetics of flumequine and in vitro activity against bacterial pathogens of gilthead sea bream Sparus aurata Journal Article Diseases of Aquatic Organisms, 54 , pp. 35 – 41, 2003. @article{rigos_pharmacokinetics_2003-1, title = {Pharmacokinetics of flumequine and in vitro activity against bacterial pathogens of gilthead sea bream Sparus aurata}, author = {G Rigos and A E Tyrpenou and I Nengas and M Yiagnisis and M Koutsodimou and M Alexis and G M Troisi}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0242500738&partnerID=40&md5=04952ed6946b3b5bf6b53e7fa6783038}, year = {2003}, date = {2003-01-01}, journal = {Diseases of Aquatic Organisms}, volume = {54}, pages = {35 -- 41}, abstract = {The present study investigated the kinetic profile of flumequine (FLU) in gilthead sea bream Sparus aurata (170 g) held at 19°C and evaluated its in vitro efficacy against important bacterial diseases in Mediterranean mariculture. Following a single intravascular injection (10 mg kg-1 fish), the distribution half-life (t1/2α) and the half-life of the terminal phase of elimination (t1/2α) of the drug were 0.2 and 30 h respectively. Tissue penetration of FLU was low, since both the apparent distribution volume of the drug at steady-state (Vd(ss)) and the apparent volume of the central compartment (Vc) were small (0.57 and 0.15 1 kg-1). The mean residence time (MRT) was short (11 h) and the total clearance (CLT) of the drug was slow (0.05 1 kg-1 h-1). Following oral administration (20 mg kg1), the bioavailability (F%) of FLU was 29% and the maximum plasma concentration was 1.7 pg ml-1. The minimum inhibitory concentration (MIC) of the drug in distilled water supplemented with 2% NaCl against Vibrio anguillarum Serotype 1b, Photobacterium damsela ssp. piscicida, V. alginolyticus, V. damsela and V. fluvialis was 0.15, 0.3, 1.2, 0.019 and 0.15 pg ml-1 respectively. The addition however of 10 mM Ca2+ and 55 mM Mg2+ to the medium resulted in an 8- to >120-fold reduction in FLU activity. The results indicate that FLU has an adequate kinetic profile in gilthead sea bream and that marine cations induce a significant impact on the activity of FLU, rendering its use against bacterial pathogens questionable.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The present study investigated the kinetic profile of flumequine (FLU) in gilthead sea bream Sparus aurata (170 g) held at 19°C and evaluated its in vitro efficacy against important bacterial diseases in Mediterranean mariculture. Following a single intravascular injection (10 mg kg-1 fish), the distribution half-life (t1/2α) and the half-life of the terminal phase of elimination (t1/2α) of the drug were 0.2 and 30 h respectively. Tissue penetration of FLU was low, since both the apparent distribution volume of the drug at steady-state (Vd(ss)) and the apparent volume of the central compartment (Vc) were small (0.57 and 0.15 1 kg-1). The mean residence time (MRT) was short (11 h) and the total clearance (CLT) of the drug was slow (0.05 1 kg-1 h-1). Following oral administration (20 mg kg1), the bioavailability (F%) of FLU was 29% and the maximum plasma concentration was 1.7 pg ml-1. The minimum inhibitory concentration (MIC) of the drug in distilled water supplemented with 2% NaCl against Vibrio anguillarum Serotype 1b, Photobacterium damsela ssp. piscicida, V. alginolyticus, V. damsela and V. fluvialis was 0.15, 0.3, 1.2, 0.019 and 0.15 pg ml-1 respectively. The addition however of 10 mM Ca2+ and 55 mM Mg2+ to the medium resulted in an 8- to >120-fold reduction in FLU activity. The results indicate that FLU has an adequate kinetic profile in gilthead sea bream and that marine cations induce a significant impact on the activity of FLU, rendering its use against bacterial pathogens questionable. |
Berrebi, P; Tsigenopoulos, C S Phylogenetic organization of the genus Barbus sensu stricto: A review based on data using molecular markers Incollection Banarescu, P; Bogutskaya, N G (Ed.): The Freshwater Fishes of Europe, Vol. 5/II: Cyprinidae, Part II: Barbus, pp. 11–22, AULA-Verlag, 2003, (Backup Publisher: AULA-Verlag). @incollection{berrebi_phylogenetic_2003, title = {Phylogenetic organization of the genus Barbus sensu stricto: A review based on data using molecular markers}, author = {P Berrebi and C S Tsigenopoulos}, editor = {P Banarescu and N G Bogutskaya}, year = {2003}, date = {2003-01-01}, booktitle = {The Freshwater Fishes of Europe, Vol. 5/II: Cyprinidae, Part II: Barbus}, pages = {11--22}, publisher = {AULA-Verlag}, abstract = {In this review, we are providing all available information on the molecular phylogeny of the genus textitBarbus sensu lato. The number of chromosomes indicates whether each species originated as diploid (50), tetraploid (100), or hexaploid (150) fishes. This is considered the primary parameter of classification. Only the "true barbs," which are the European, West Asian, and North African tetraploids, are discussed in the text species by species. Special care is taken to provide an accurate phylogeny of the European species. The main conclusion reached through the molecular studies is that it is necessary to limit the genus textitBarbus to the "true barbs" and to give different generic names to all other taxa originally included in textitBarbus sensu lato}, note = {Backup Publisher: AULA-Verlag}, keywords = {}, pubstate = {published}, tppubtype = {incollection} } In this review, we are providing all available information on the molecular phylogeny of the genus textitBarbus sensu lato. The number of chromosomes indicates whether each species originated as diploid (50), tetraploid (100), or hexaploid (150) fishes. This is considered the primary parameter of classification. Only the "true barbs," which are the European, West Asian, and North African tetraploids, are discussed in the text species by species. Special care is taken to provide an accurate phylogeny of the European species. The main conclusion reached through the molecular studies is that it is necessary to limit the genus textitBarbus to the "true barbs" and to give different generic names to all other taxa originally included in textitBarbus sensu lato |
Tsigenopoulos, C S; Durand, J D; Ünlü, E; Berrebi, P Biological Journal of the Linnean Society, 80 , pp. 207 – 222, 2003. @article{tsigenopoulos_rapid_2003, title = {Rapid radiation of the Mediterranean Luciobarbus species (Cyprinidae) after the Messinian salinity crisis of the Mediterranean Sea, inferred from mitochondrial phylogenetic analysis}, author = {C S Tsigenopoulos and J D Durand and E Ünlü and P Berrebi}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0142095131&partnerID=40&md5=b366bb24a4425150565d56b1f334c4ee}, year = {2003}, date = {2003-01-01}, journal = {Biological Journal of the Linnean Society}, volume = {80}, pages = {207 -- 222}, abstract = {Phylogenetic relationships of 64 freshwater Barbus s.s. species distributed in basins around the Mediterranean Sea were assessed using cytochrome b sequences. Our results are in concordance with previous morphological and genetic studies, which proposed that these species belong to two major lineages (or subgenera): Barbus and Luciobarbus. We were particularly interested in phylogenetic relationships among species of the Luciobarbus lineage that are primarily found in the southern Mediterranean region from the Iberian Peninsula to the Middle East. In the Luciobarbus lineage, species that were previously attributed to the Capoeta genus were clustered. In this study, we observed short internodes between monophyletic groups having a geographical agreement around the Mediterranean. However, groups from the opposite sides of the Mediterranean Sea (Iberian Peninsula-Capoeta, north-western Africa-Middle East) seem to be phylogenetically close. We therefore infer that rapid radiation of Luciobarbus species in the Late Miocene better fits our data rather than gradual founder events in the southern Mediterranean. We propose that the biogeographical event along an east-west route, responsible for the present distribution of Luciobarbus species, was the ’Lago Mare’ phase of the Mediterranean Sea that provided a rapid dispersal route over extensive distances. This provides new insights into the speciation pattern of this group, and may be of general use in the study of freshwater species in these regions. © 2003 The Linnean Society of London.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Phylogenetic relationships of 64 freshwater Barbus s.s. species distributed in basins around the Mediterranean Sea were assessed using cytochrome b sequences. Our results are in concordance with previous morphological and genetic studies, which proposed that these species belong to two major lineages (or subgenera): Barbus and Luciobarbus. We were particularly interested in phylogenetic relationships among species of the Luciobarbus lineage that are primarily found in the southern Mediterranean region from the Iberian Peninsula to the Middle East. In the Luciobarbus lineage, species that were previously attributed to the Capoeta genus were clustered. In this study, we observed short internodes between monophyletic groups having a geographical agreement around the Mediterranean. However, groups from the opposite sides of the Mediterranean Sea (Iberian Peninsula-Capoeta, north-western Africa-Middle East) seem to be phylogenetically close. We therefore infer that rapid radiation of Luciobarbus species in the Late Miocene better fits our data rather than gradual founder events in the southern Mediterranean. We propose that the biogeographical event along an east-west route, responsible for the present distribution of Luciobarbus species, was the ’Lago Mare’ phase of the Mediterranean Sea that provided a rapid dispersal route over extensive distances. This provides new insights into the speciation pattern of this group, and may be of general use in the study of freshwater species in these regions. © 2003 The Linnean Society of London. |
Mylonas, C C; Papadaki, M; Divanach, P Seasonal changes in sperm production and quality in the red porgy Pagrus pagrus (L.) Journal Article Aquaculture Research, 34 (13), pp. 1161–1170, 2003, ISSN: 1355557X. @article{mylonas_seasonal_2003, title = {Seasonal changes in sperm production and quality in the red porgy Pagrus pagrus (L.)}, author = {C C Mylonas and M Papadaki and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0242286610&doi=10.1046%2fj.1365-2109.2003.00922.x&partnerID=40&md5=035f37a6d19b84e81a7004fb96f03fec}, doi = {10.1046/j.1365-2109.2003.00922.x}, issn = {1355557X}, year = {2003}, date = {2003-01-01}, journal = {Aquaculture Research}, volume = {34}, number = {13}, pages = {1161--1170}, abstract = {Cultured red porgy Pagrus pagrus (L.) males (n = 6) were sampled every 2 weeks for milt, in order to monitor changes in sperm quality parameters during a whole spawning period. On 11 January 2001, 60% of the fish were spermiating, increasing to 100% in mid-February and dropping to 30% by mid-April. Sperm density showed a slight increasing trend, with mean values ranging between 8.6 and 23.7 × 109 spermatozoa mL-1. Sperm motility percentage exhibited a significant improvement during the spawning season (analysis of variance (ANOVA) P = 0.0001). The duration of forward motility for the major part of the monitoring period ranged between 2 and 4 min. Red porgy spermatozoa maintained their viability for many days after whole storage of milt at 4°C. During the monitoring period there were significant changes in the mean duration of sperm survival after cold storage, ranging from 5 to 12 days. The total volume of expressible milt was maximal on 28 March, increasing from a mean value of 1.7 mL to 5.3 mL kg-1. Milt production of captive-reared red porgy does not appear to be limiting, when compared with the volume of expressible milt produced by other cultured marine fishes.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Cultured red porgy Pagrus pagrus (L.) males (n = 6) were sampled every 2 weeks for milt, in order to monitor changes in sperm quality parameters during a whole spawning period. On 11 January 2001, 60% of the fish were spermiating, increasing to 100% in mid-February and dropping to 30% by mid-April. Sperm density showed a slight increasing trend, with mean values ranging between 8.6 and 23.7 × 109 spermatozoa mL-1. Sperm motility percentage exhibited a significant improvement during the spawning season (analysis of variance (ANOVA) P = 0.0001). The duration of forward motility for the major part of the monitoring period ranged between 2 and 4 min. Red porgy spermatozoa maintained their viability for many days after whole storage of milt at 4°C. During the monitoring period there were significant changes in the mean duration of sperm survival after cold storage, ranging from 5 to 12 days. The total volume of expressible milt was maximal on 28 March, increasing from a mean value of 1.7 mL to 5.3 mL kg-1. Milt production of captive-reared red porgy does not appear to be limiting, when compared with the volume of expressible milt produced by other cultured marine fishes. |
Machias, A; Somarakis, S; Papandroulakis, N; Spedicato, M -T; Suquet, M; Lembo, G; Divanach, P Settlement of the wreckfish (Polyprion americanus) Journal Article Marine Biology, 142 (1), pp. 45–52, 2003, ISSN: 0025-3162. @article{machias_settlement_2003, title = {Settlement of the wreckfish (Polyprion americanus)}, author = {A Machias and S Somarakis and N Papandroulakis and M -T Spedicato and M Suquet and G Lembo and P Divanach}, url = {http://link.springer.com/10.1007/s00227-002-0918-2}, doi = {10.1007/s00227-002-0918-2}, issn = {0025-3162}, year = {2003}, date = {2003-01-01}, urldate = {2020-08-18}, journal = {Marine Biology}, volume = {142}, number = {1}, pages = {45--52}, abstract = {The wreckfish (Polyprion americanus) is a panoceanic, long-lived, deep-water demersal species, characterized by an extended pelagic juvenile phase associated with floating objects. In the present study morphometric data from 435 specimens collected from Greece (Crete), Italy (Ionian Sea) and the Atlantic coast of France from September 1999 to March 2001 were analyzed to estimate the settlement size of the species and associated changes in morphology. The fishery sample included specimens from both the pelagic and the demersal stage. Length-at-settlement (TL50) and 95% confidence intervals were estimated by fitting a logistic function. Eleven morphometric characters were analyzed, and the existing inflection points, reflecting alterations in body shape, were defined: (1) iteratively, as the transition point, splitting the data set into two groups, for which the reduced major axis functions, between a character and total length, of the successive groups best fit (minimum sum of squares of residuals) the combined data set and (2) where the second derivative of the fitted third-order polynomial functions to morphometric ratios equaled zero. The main size range within which wreckfish settled was 56-65 cm, and the means of the inflection points defined by the two methods were 61 and 64 cm. These results indicate changes in functional morphology associated with settlement. The monitoring of wreckfish juveniles caught in the wild and kept individually in captivity showed changes in growth and food intake at approximately the estimated settlement length and suggested that temperature was the most likely factor triggering settlement.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The wreckfish (Polyprion americanus) is a panoceanic, long-lived, deep-water demersal species, characterized by an extended pelagic juvenile phase associated with floating objects. In the present study morphometric data from 435 specimens collected from Greece (Crete), Italy (Ionian Sea) and the Atlantic coast of France from September 1999 to March 2001 were analyzed to estimate the settlement size of the species and associated changes in morphology. The fishery sample included specimens from both the pelagic and the demersal stage. Length-at-settlement (TL50) and 95% confidence intervals were estimated by fitting a logistic function. Eleven morphometric characters were analyzed, and the existing inflection points, reflecting alterations in body shape, were defined: (1) iteratively, as the transition point, splitting the data set into two groups, for which the reduced major axis functions, between a character and total length, of the successive groups best fit (minimum sum of squares of residuals) the combined data set and (2) where the second derivative of the fitted third-order polynomial functions to morphometric ratios equaled zero. The main size range within which wreckfish settled was 56-65 cm, and the means of the inflection points defined by the two methods were 61 and 64 cm. These results indicate changes in functional morphology associated with settlement. The monitoring of wreckfish juveniles caught in the wild and kept individually in captivity showed changes in growth and food intake at approximately the estimated settlement length and suggested that temperature was the most likely factor triggering settlement. |
Prinos, P; Koutandos, E; Karambas, T Spectral evolution of breaking-non breaking waves in the surf zone Inproceedings pp. 312–313, 2003, (Publication Title: 29th International Conference on Coastal Engineering). @inproceedings{prinos_spectral_2003, title = {Spectral evolution of breaking-non breaking waves in the surf zone}, author = {P Prinos and E Koutandos and T Karambas}, year = {2003}, date = {2003-01-01}, volume = {1}, pages = {312--313}, note = {Publication Title: 29th International Conference on Coastal Engineering}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Naletaki, M; Koutsoubas, D; Dounas, C; Arvanitidis, C Structure and dynamics of the macrobenthic crustaceans in the Gialova lagoon (SW Peloponnese) Inproceedings Chersonisos, Crete, Greece, 2003, (Publication Title: Seventh Symposium on Oceanography and Fisheries). @inproceedings{naletaki_structure_2003, title = {Structure and dynamics of the macrobenthic crustaceans in the Gialova lagoon (SW Peloponnese)}, author = {M Naletaki and D Koutsoubas and C Dounas and C Arvanitidis}, year = {2003}, date = {2003-01-01}, address = {Chersonisos, Crete, Greece}, note = {Publication Title: Seventh Symposium on Oceanography and Fisheries}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Arvanitidis, C; Bellan, G; Drakopoulos, P; Valavanis, V; Dounas, C; Koukouras, A; Eleftheriou, A Testing hypotheses on the biodiversity patterns occurring in the Mediterranean and Black Seas: the example of the benthic polychaetes Inproceedings Chersonisos, Crete, Greece, 2003, (Publication Title: Seventh Symposium on Oceanography and Fisheries). @inproceedings{arvanitidis_testing_2003, title = {Testing hypotheses on the biodiversity patterns occurring in the Mediterranean and Black Seas: the example of the benthic polychaetes}, author = {C Arvanitidis and G Bellan and P Drakopoulos and V Valavanis and C Dounas and A Koukouras and A Eleftheriou}, year = {2003}, date = {2003-01-01}, address = {Chersonisos, Crete, Greece}, note = {Publication Title: Seventh Symposium on Oceanography and Fisheries}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Heip, C; Costello, M; Hummel, H; Avesaath, Van P; Arvanitidis, C; Eleftheriou, A The status of Marine Biodiversity in Europe Inproceedings Arvanitidis, C; as representative of, MARBENA Consortium (Ed.): ΙΑSON: International Conference on the Sustainable Development of the Mediterranean and Black Sea Environment, Thessaloniki, Greece, 2003. @inproceedings{heip_status_2003, title = {The status of Marine Biodiversity in Europe}, author = {C Heip and M Costello and H Hummel and P Van Avesaath and C Arvanitidis and A Eleftheriou}, editor = {C Arvanitidis and MARBENA Consortium as representative of}, year = {2003}, date = {2003-01-01}, booktitle = {ΙΑSON: International Conference on the Sustainable Development of the Mediterranean and Black Sea Environment}, address = {Thessaloniki, Greece}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Rigos, G; Nengas, I; Alexis, M; Tyrpenou, A E; Troisi, G M Aquaculture, 224 , pp. 245 – 256, 2003. @article{rigos_tissue_2003, title = {Tissue distribution and residue depletion of oxolinic acid in gilthead sea bream (Sparus aurata) and sharpsnout sea bream (Diplodus puntazzo) following multiple in-feed dosing}, author = {G Rigos and I Nengas and M Alexis and A E Tyrpenou and G M Troisi}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0037911446&partnerID=40&md5=9b91d50c3055d3d2fde33418539e5e5f}, year = {2003}, date = {2003-01-01}, journal = {Aquaculture}, volume = {224}, pages = {245 -- 256}, abstract = {The tissue distribution and residue depletion of oxolinic acid (OA) were investigated in gilthead sea bream and sharpsnout sea bream after a multiple (10 days) in-feed administration (30 mg/kg fish) at 19°C. Plasma, muscle, skin, liver and bile were sampled daily during treatment (1-10 days) and post-treatment (11,12, 14, 15 and 17 days) and subsequently analysed for OA concentrations using high-performance liquid chromatography and fluorescent detection (HPLC-SFD). Oxolinic acid displayed biphasic elimination in tissues of both species during treatment and reached "consumer safe levels" 24 h after treatment. OA concentration in gilthead sea bream peaked on day 4, with highest concentrations in liver (2660 ng/g), followed by bile (2370 ng/ml), skin (2350 ng/g), muscle (1330 ng/g) and plasma (880 ng/ml). In sharpsnout sea bream, peaked OA concentrations were observed in liver (2720 ng/g) on day 4, while highest concentrations were measured later on day 5 in plasma (910 ng/ml) and day 6 in bile (2360 ng/ml), skin (1960 ng/g) and muscle (1770 ng/g). The calculated areas under the concentration curves (AUC) also reflect a good tissue distribution of OA. Elimination half-lives (t1/2) were estimated to be 11-14 and 11-19 h in tissues of gilthead sea bream and sharpsnout sea bream, respectively. Multiple dosing of OA in sparids should be adequate to confront bacterial pathogens considering published minimum inhibitory concentrations of the drug. © 2003 Elsevier Science B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The tissue distribution and residue depletion of oxolinic acid (OA) were investigated in gilthead sea bream and sharpsnout sea bream after a multiple (10 days) in-feed administration (30 mg/kg fish) at 19°C. Plasma, muscle, skin, liver and bile were sampled daily during treatment (1-10 days) and post-treatment (11,12, 14, 15 and 17 days) and subsequently analysed for OA concentrations using high-performance liquid chromatography and fluorescent detection (HPLC-SFD). Oxolinic acid displayed biphasic elimination in tissues of both species during treatment and reached "consumer safe levels" 24 h after treatment. OA concentration in gilthead sea bream peaked on day 4, with highest concentrations in liver (2660 ng/g), followed by bile (2370 ng/ml), skin (2350 ng/g), muscle (1330 ng/g) and plasma (880 ng/ml). In sharpsnout sea bream, peaked OA concentrations were observed in liver (2720 ng/g) on day 4, while highest concentrations were measured later on day 5 in plasma (910 ng/ml) and day 6 in bile (2360 ng/ml), skin (1960 ng/g) and muscle (1770 ng/g). The calculated areas under the concentration curves (AUC) also reflect a good tissue distribution of OA. Elimination half-lives (t1/2) were estimated to be 11-14 and 11-19 h in tissues of gilthead sea bream and sharpsnout sea bream, respectively. Multiple dosing of OA in sparids should be adequate to confront bacterial pathogens considering published minimum inhibitory concentrations of the drug. © 2003 Elsevier Science B.V. All rights reserved. |
Stephanou, E G; Mandalakis, M; Apostolaki, M Transport and Atmospheric Processes PCBs in the subtropical atmosphere of Eastern Mediterranean Inproceedings Goldschmidt Conference Abstracts, pp. 18, 447, 2003. @inproceedings{stephanou_transport_2003, title = {Transport and Atmospheric Processes PCBs in the subtropical atmosphere of Eastern Mediterranean}, author = {E G Stephanou and M Mandalakis and M Apostolaki}, year = {2003}, date = {2003-01-01}, booktitle = {Goldschmidt Conference Abstracts}, volume = {67}, pages = {18, 447}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
2002 |
Alasalvar, C; Taylor, K D A; Zubcov, E; Shahidi, F; Alexis, M Differentiation of cultured and wild sea bass (Dicentrarchus labrax): total lipid content, fatty acid and trace mineral composition Journal Article Food Chemistry, 79 (2), pp. 145–150, 2002, ISSN: 03088146. @article{alasalvar_differentiation_2002, title = {Differentiation of cultured and wild sea bass (Dicentrarchus labrax): total lipid content, fatty acid and trace mineral composition}, author = {C Alasalvar and K D A Taylor and E Zubcov and F Shahidi and M Alexis}, url = {https://linkinghub.elsevier.com/retrieve/pii/S030881460200122X}, doi = {10.1016/S0308-8146(02)00122-X}, issn = {03088146}, year = {2002}, date = {2002-11-01}, urldate = {2020-08-17}, journal = {Food Chemistry}, volume = {79}, number = {2}, pages = {145--150}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Koumoundouros, G; Maingot, E; Divanach, P; Kentouri, M Kyphosis in reared sea bass (Dicentrarchus labrax L.): ontogeny and effects on mortality Journal Article Aquaculture, 209 (1-4), pp. 49–58, 2002, ISSN: 00448486. @article{koumoundouros_kyphosis_2002, title = {Kyphosis in reared sea bass (Dicentrarchus labrax L.): ontogeny and effects on mortality}, author = {G Koumoundouros and E Maingot and P Divanach and M Kentouri}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848601008213}, doi = {10.1016/S0044-8486(01)00821-3}, issn = {00448486}, year = {2002}, date = {2002-06-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {209}, number = {1-4}, pages = {49--58}, abstract = {The ontogeny and the effects of vertebral kyphosis on the mortality rate of reared sea bass (Dicentrarchus labrax) are described. The first evidence of deformities was observed in individuals of 10 mm total length (TL) in the form of light malformations or bilateral asymmetries of individual vertebrae. At 14 mm mean TL, the 18.2% of the reared fish presented severe vertebral kyphosis at the pre-haemal region. Kyphosis continued to develop and presented its maximum incidence at 17 mm TL (24.8%). The centre of the deformity was mainly located on vertebrae 5-6. Kyphotic specimens presented a lethargic behaviour. After 17 mm mean TL, the incidence of the deformity decreased exponentially over time due to the heavy mortality of the affected fish (24.8% kyphosis incidence in the surviving fish vs. 52.6% in the dead fish collected from the tank, p textless 0.01). For the first time, it was shown that although a skeletal deformity could have little effect on the final quality of the reared juveniles, it can significantly affect the mortality rate and thus the productivity of the hatcheries. The results are discussed with respect to the aetiology, applications and research targets for the erasure of skeletal malformations from the reared fish. © 2002 Elsevier Science B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The ontogeny and the effects of vertebral kyphosis on the mortality rate of reared sea bass (Dicentrarchus labrax) are described. The first evidence of deformities was observed in individuals of 10 mm total length (TL) in the form of light malformations or bilateral asymmetries of individual vertebrae. At 14 mm mean TL, the 18.2% of the reared fish presented severe vertebral kyphosis at the pre-haemal region. Kyphosis continued to develop and presented its maximum incidence at 17 mm TL (24.8%). The centre of the deformity was mainly located on vertebrae 5-6. Kyphotic specimens presented a lethargic behaviour. After 17 mm mean TL, the incidence of the deformity decreased exponentially over time due to the heavy mortality of the affected fish (24.8% kyphosis incidence in the surviving fish vs. 52.6% in the dead fish collected from the tank, p textless 0.01). For the first time, it was shown that although a skeletal deformity could have little effect on the final quality of the reared juveniles, it can significantly affect the mortality rate and thus the productivity of the hatcheries. The results are discussed with respect to the aetiology, applications and research targets for the erasure of skeletal malformations from the reared fish. © 2002 Elsevier Science B.V. All rights reserved. |
Koumoundouros, George; Pavlidis, Michalis; Anezaki, Lina; Kokkari, Constantina; Sterioti, Aspasia; Divanach, Pascal; Kentouri, Maroudio Journal of Experimental Zoology, 292 (6), pp. 573–579, 2002, ISSN: 0022-104X, 1097-010X. @article{koumoundouros_temperature_2002, title = {Temperature sex determination in the European sea bass, Dicentrarchus labrax (L., 1758) (Teleostei, Perciformes, Moronidae): Critical sensitive ontogenetic phase}, author = {George Koumoundouros and Michalis Pavlidis and Lina Anezaki and Constantina Kokkari and Aspasia Sterioti and Pascal Divanach and Maroudio Kentouri}, url = {http://doi.wiley.com/10.1002/jez.10095}, doi = {10.1002/jez.10095}, issn = {0022-104X, 1097-010X}, year = {2002}, date = {2002-05-01}, urldate = {2020-08-18}, journal = {Journal of Experimental Zoology}, volume = {292}, number = {6}, pages = {573--579}, abstract = {The temperature sex determination (TSD) mechanism in the European sea bass (Dicentrarchus labrax L.) was studied in respect to: a) the TSD sensitivity during the different developmental stages; and b) the intrapopulation correlation of sex determination with the growth rate up to the end of the TSD-sensitive period. At the stage of half-epiboly, eggs from the same batch were divided into four groups and subjected to different thermal treatments: a) 15°C (G15 group) and b) 20°C (G20 group) up to the middle of metamorphosis stage; c) 15°C up to the end of yolk-sac larval stage and subsequently to 20°C (G15-5 group); and d) 15°C up to the end of the preflexion stage and then to 20°C (G15-10 group). At the end of the treatments, size grading was applied and four additional populations were established from the upper (L) and lower (S) size portions of the G15 and G20 populations: G15L, G15S, G20L, and G20S. During the following growing phase, all populations were subjected to common rearing conditions. The sex ratios of each population were macroscopically determined at 190-210 mm mean total length. Female incidence was significantly affected (P textless 0.05) by the different thermal treatments: 66.1% in the G15, 47.1% in the G15-10, 37.6% in the G15-5, and 18.1% in the G20 group. In addition, sex ratio was correlated with the growth rate of the fish up to the end of the TSD-sensitive period, with the larger fish presenting a significantly higher (P textless 0.01) female incidence than the smaller fish in both thermal regimes tested: 73.1% in G15L vs. 57% in G15S, and 36.6%in G20L vs. 22.5% in G20S group. Results provide, for the first time, clear evidence that the sea bass is sensitive to TSD during all different ontogenetic stages up to metamorphosis, and that sex ratio is correlated with the growth rate of the fish well before the differentiation and maturation of the gonads. © 2002 Wiley-Liss, Inc.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The temperature sex determination (TSD) mechanism in the European sea bass (Dicentrarchus labrax L.) was studied in respect to: a) the TSD sensitivity during the different developmental stages; and b) the intrapopulation correlation of sex determination with the growth rate up to the end of the TSD-sensitive period. At the stage of half-epiboly, eggs from the same batch were divided into four groups and subjected to different thermal treatments: a) 15°C (G15 group) and b) 20°C (G20 group) up to the middle of metamorphosis stage; c) 15°C up to the end of yolk-sac larval stage and subsequently to 20°C (G15-5 group); and d) 15°C up to the end of the preflexion stage and then to 20°C (G15-10 group). At the end of the treatments, size grading was applied and four additional populations were established from the upper (L) and lower (S) size portions of the G15 and G20 populations: G15L, G15S, G20L, and G20S. During the following growing phase, all populations were subjected to common rearing conditions. The sex ratios of each population were macroscopically determined at 190-210 mm mean total length. Female incidence was significantly affected (P textless 0.05) by the different thermal treatments: 66.1% in the G15, 47.1% in the G15-10, 37.6% in the G15-5, and 18.1% in the G20 group. In addition, sex ratio was correlated with the growth rate of the fish up to the end of the TSD-sensitive period, with the larger fish presenting a significantly higher (P textless 0.01) female incidence than the smaller fish in both thermal regimes tested: 73.1% in G15L vs. 57% in G15S, and 36.6%in G20L vs. 22.5% in G20S group. Results provide, for the first time, clear evidence that the sea bass is sensitive to TSD during all different ontogenetic stages up to metamorphosis, and that sex ratio is correlated with the growth rate of the fish well before the differentiation and maturation of the gonads. © 2002 Wiley-Liss, Inc. |
Koumoundouros, G; Sfakianakis, D G; Divanach, P; Kentouri, M Effect of temperature on swimming performance of sea bass juveniles Journal Article Journal of Fish Biology, 60 (4), pp. 923–932, 2002, ISSN: 0022-1112, 1095-8649. @article{koumoundouros_effect_2002, title = {Effect of temperature on swimming performance of sea bass juveniles}, author = {G Koumoundouros and D G Sfakianakis and P Divanach and M Kentouri}, url = {http://doi.wiley.com/10.1111/j.1095-8649.2002.tb02418.x}, doi = {10.1111/j.1095-8649.2002.tb02418.x}, issn = {0022-1112, 1095-8649}, year = {2002}, date = {2002-04-01}, urldate = {2020-08-18}, journal = {Journal of Fish Biology}, volume = {60}, number = {4}, pages = {923--932}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ladoukakis, E D; Saavedra, C; Magoulas, A; Zouros, E Molecular Ecology, 11 (4), pp. 755–769, 2002, ISSN: 0962-1083, 1365-294X. @article{ladoukakis_mitochondrial_2002, title = {Mitochondrial DNA variation in a species with two mitochondrial genomes: the case of Mytilus galloprovincialis from the Atlantic, the Mediterranean and the Black Sea}, author = {E D Ladoukakis and C Saavedra and A Magoulas and E Zouros}, url = {http://doi.wiley.com/10.1046/j.1365-294X.2002.01473.x}, doi = {10.1046/j.1365-294X.2002.01473.x}, issn = {0962-1083, 1365-294X}, year = {2002}, date = {2002-04-01}, urldate = {2020-08-09}, journal = {Molecular Ecology}, volume = {11}, number = {4}, pages = {755--769}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Papandroulakis, Nikos; Papaioannou, Dimitris; Divanach, Pascal An automated feeding system for intensive hatcheries Journal Article Aquacultural Engineering, 26 (1), pp. 13–26, 2002, ISSN: 01448609. @article{papandroulakis_automated_2002, title = {An automated feeding system for intensive hatcheries}, author = {Nikos Papandroulakis and Dimitris Papaioannou and Pascal Divanach}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0144860901000917}, doi = {10.1016/S0144-8609(01)00091-7}, issn = {01448609}, year = {2002}, date = {2002-03-01}, urldate = {2020-08-06}, journal = {Aquacultural Engineering}, volume = {26}, number = {1}, pages = {13--26}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Tsigenopoulos, Costas S; Kotlík, Petr; Berrebi, Patrick Biological Journal of the Linnean Society, 75 (1), pp. 83–99, 2002, ISSN: 00244066, 10958312. @article{tsigenopoulos_biogeography_2002, title = {Biogeography and pattern of gene flow among Barbus species (Teleostei: Cyprinidae) inhabiting the Italian Peninsula and neighbouring Adriatic drainages as revealed by allozyme and mitochondrial sequence data}, author = {Costas S Tsigenopoulos and Petr Kotlík and Patrick Berrebi}, url = {https://academic.oup.com/biolinnean/article-lookup/doi/10.1046/j.1095-8312.2002.00007.x}, doi = {10.1046/j.1095-8312.2002.00007.x}, issn = {00244066, 10958312}, year = {2002}, date = {2002-03-01}, urldate = {2020-08-10}, journal = {Biological Journal of the Linnean Society}, volume = {75}, number = {1}, pages = {83--99}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Hatziathanasiou, A; Paspatis, M; Houbart, M; Kestemont, P; Stefanakis, S; Kentouri, M Aquaculture, 205 (1-2), pp. 89–102, 2002, ISSN: 00448486. @article{hatziathanasiou_survival_2002, title = {Survival, growth and feeding in early life stages of European sea bass (Dicentrarchus labrax) intensively cultured under different stocking densities}, author = {A Hatziathanasiou and M Paspatis and M Houbart and P Kestemont and S Stefanakis and M Kentouri}, url = {https://linkinghub.elsevier.com/retrieve/pii/S004484860100672X}, doi = {10.1016/S0044-8486(01)00672-X}, issn = {00448486}, year = {2002}, date = {2002-02-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {205}, number = {1-2}, pages = {89--102}, abstract = {Two experiments were conducted in triplicate in order to study the effect of stocking densities on survival (highlighting sibling cannibalism), growth and feeding of intensively cultured sea bass larvae (50, 100, 150 and 200 fish 1-1) and post-larvae (5, 10, 15 and 20 fish 1-1). Experimental populations were reared under controlled conditions in 50-1 cylindroconical tanks. Dead fish were counted daily and classified into cannibalised and non-cannibalised. Total length and weight were measured weekly. Results indicate that stocking density did not affect survival and growth of larvae. No cannibalistic phenomena were observed at this stage. On the other hand, survival of post-larvae was higher at 5 and 10 fish 1-1 than at 15 and 20 fish 1-1, while growth performance fluctuated between the lowest value recorded in the group of 10 fish 1-1 and the highest value in that of 5 fish 1-1. Feed intake in post-larvae was independent of stocking density. Cannibalism was the main cause of death in post-larvae. Two types of cannibalism were detected: type I, attack from tail (observed at the beginning of this stage) and type II, attack from head (observed at the end of the stage). © 2002 Elsevier Science B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Two experiments were conducted in triplicate in order to study the effect of stocking densities on survival (highlighting sibling cannibalism), growth and feeding of intensively cultured sea bass larvae (50, 100, 150 and 200 fish 1-1) and post-larvae (5, 10, 15 and 20 fish 1-1). Experimental populations were reared under controlled conditions in 50-1 cylindroconical tanks. Dead fish were counted daily and classified into cannibalised and non-cannibalised. Total length and weight were measured weekly. Results indicate that stocking density did not affect survival and growth of larvae. No cannibalistic phenomena were observed at this stage. On the other hand, survival of post-larvae was higher at 5 and 10 fish 1-1 than at 15 and 20 fish 1-1, while growth performance fluctuated between the lowest value recorded in the group of 10 fish 1-1 and the highest value in that of 5 fish 1-1. Feed intake in post-larvae was independent of stocking density. Cannibalism was the main cause of death in post-larvae. Two types of cannibalism were detected: type I, attack from tail (observed at the beginning of this stage) and type II, attack from head (observed at the end of the stage). © 2002 Elsevier Science B.V. All rights reserved. |
Kantzaris, V; Iliopoulou-Georgudaki, J; Katharios, P; Kaspiris, P A comparison of several biotic indices used for water quality assessment at the Greek rivers Journal Article Fresenius Environmental Bulletin, 11 (11), pp. 1000–1007, 2002, ISSN: 10184619. @article{kantzaris_comparison_2002, title = {A comparison of several biotic indices used for water quality assessment at the Greek rivers}, author = {V Kantzaris and J Iliopoulou-Georgudaki and P Katharios and P Kaspiris}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036869322&partnerID=40&md5=63791a418eaa87d734878c737cd2cf66}, issn = {10184619}, year = {2002}, date = {2002-01-01}, journal = {Fresenius Environmental Bulletin}, volume = {11}, number = {11}, pages = {1000--1007}, abstract = {Nine biotic indices and benthic macroinvertebrates scores used for the assessment of water quality over a period of one year at the rivers Kalamas and Alfeios (Greece) were compared. The results of the biotic indexes were combined with the measurements of physicochemical parameters, in order to assess the water quality and to find the most applicable index for assessing Greek rivers. The indexes BBI and IBE were found to be more suitable. However, the need for the establishment of a Greek Biotic Index for assessment of the rivers in the Greek region is marked out.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Nine biotic indices and benthic macroinvertebrates scores used for the assessment of water quality over a period of one year at the rivers Kalamas and Alfeios (Greece) were compared. The results of the biotic indexes were combined with the measurements of physicochemical parameters, in order to assess the water quality and to find the most applicable index for assessing Greek rivers. The indexes BBI and IBE were found to be more suitable. However, the need for the establishment of a Greek Biotic Index for assessment of the rivers in the Greek region is marked out. |
Arvanitidis, C; Koutsoubas, D; Robin, J -P; Pereira, J; Moreno, A; Cunha, Da M M; Valavanis, V; Eleftheriou, A Bulletin of Marine Science, 71 (1), pp. 129–146, 2002, ISSN: 00074977. @article{arvanitidis_comparison_2002, title = {A comparison of the fishery biology of three Illex coindetii Vérany, 1839 (Cephalopoda: Ommastrephidae) populations from the European Atlantic and Mediterranean waters}, author = {C Arvanitidis and D Koutsoubas and J -P Robin and J Pereira and A Moreno and M M Da Cunha and V Valavanis and A Eleftheriou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0038066629&partnerID=40&md5=28717355f1e10d2ec68ace387c5860bc}, issn = {00074977}, year = {2002}, date = {2002-01-01}, journal = {Bulletin of Marine Science}, volume = {71}, number = {1}, pages = {129--146}, abstract = {Three populations of the short-finned squid Illex coindetii were simultaneously sampled and studied from the Southern Celtic Sea and Bay of Biscay, Portuguese waters and Greek Seas, during a EU funded project (FAIR CT 1520). Dorsal mantle length (DML) ranged from 24 mm to 360 mm and body weight (BW) from 2.4 g to 1630 g. Modal progression analysis (MPA) indicated a female life span of 13-18 mo and in male life span of 11 mo. Length-weight relationship was found to be positively allometric for males and negatively allometric for females in all three populations. Sex ratio did not differ significantly from 1:1 over the entire sampling period. Differences among the three populations were observed for the following life-cycle parameters: (1) males from the Portuguese waters had significantly different length-weight slopes from those from the remainder areas; (2) size at full recruitment was determined at 110 mm in females from the Portuguese waters whereas in females from the Southern Celtic Sea and Bay of Biscay and from the Greek Seas was 170 mm; (3) female recruitment peaks were recorded only during autumn in samples from the Southern Celtic Sea and Bay of Biscay while in those from the Portuguese waters and the Greek Seas the recruitment peaks were recorded at various seasons; (4) maturing and mature males were found abundantly over the entire sampling period in the samples from the Southern Celtic Sea and Bay of Biscay and the Greek Seas whereas in those from the Portuguese waters maturing and mature males were at higher percentages than immature ones only during spring and summer; peaks of the Gonadosomatic index, calculated for mature females from the Southern Celtic Sea and Bay of Biscay, were scored from February to April of 1998 and 1999 and during September of 1998 while in the samples from the Greek waters during April and August 1998 and during January and May 1999; (5) L50 (mantle length at which 50% of the individuals are mature) values estimated for both females and males from the three studied areas were gradually decreased from the North Atlantic to the Mediterranean; (6) length data suggest that females from the Greek Seas potentially mature at two modal sizes while those from the remainder areas show only one modal size at maturity. Environmental variables (sea surface temperature and chlorophyll-a concentration) were correlated with some of the biological indices of the I. coindetii populations. Finally, results on biological indices of the I. coindetii are compared with those calculated for its congeneric species I. illecebrosus, I. argentinus as well as with another European ommastrephid species Todaropsis eblanae.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Three populations of the short-finned squid Illex coindetii were simultaneously sampled and studied from the Southern Celtic Sea and Bay of Biscay, Portuguese waters and Greek Seas, during a EU funded project (FAIR CT 1520). Dorsal mantle length (DML) ranged from 24 mm to 360 mm and body weight (BW) from 2.4 g to 1630 g. Modal progression analysis (MPA) indicated a female life span of 13-18 mo and in male life span of 11 mo. Length-weight relationship was found to be positively allometric for males and negatively allometric for females in all three populations. Sex ratio did not differ significantly from 1:1 over the entire sampling period. Differences among the three populations were observed for the following life-cycle parameters: (1) males from the Portuguese waters had significantly different length-weight slopes from those from the remainder areas; (2) size at full recruitment was determined at 110 mm in females from the Portuguese waters whereas in females from the Southern Celtic Sea and Bay of Biscay and from the Greek Seas was 170 mm; (3) female recruitment peaks were recorded only during autumn in samples from the Southern Celtic Sea and Bay of Biscay while in those from the Portuguese waters and the Greek Seas the recruitment peaks were recorded at various seasons; (4) maturing and mature males were found abundantly over the entire sampling period in the samples from the Southern Celtic Sea and Bay of Biscay and the Greek Seas whereas in those from the Portuguese waters maturing and mature males were at higher percentages than immature ones only during spring and summer; peaks of the Gonadosomatic index, calculated for mature females from the Southern Celtic Sea and Bay of Biscay, were scored from February to April of 1998 and 1999 and during September of 1998 while in the samples from the Greek waters during April and August 1998 and during January and May 1999; (5) L50 (mantle length at which 50% of the individuals are mature) values estimated for both females and males from the three studied areas were gradually decreased from the North Atlantic to the Mediterranean; (6) length data suggest that females from the Greek Seas potentially mature at two modal sizes while those from the remainder areas show only one modal size at maturity. Environmental variables (sea surface temperature and chlorophyll-a concentration) were correlated with some of the biological indices of the I. coindetii populations. Finally, results on biological indices of the I. coindetii are compared with those calculated for its congeneric species I. illecebrosus, I. argentinus as well as with another European ommastrephid species Todaropsis eblanae. |
Plaiti, W; Sardá, F; Tselepides, A A comperative study of the deep-sea epibenthic fauna from the Balearic (NW Mediterranean) and Ionian (SE Mwediterranean) Basins. Inproceedings Lodz, Poland., 2002, (Publication Title: The 4th European Crustacean Conference.). @inproceedings{plaiti_comperative_2002, title = {A comperative study of the deep-sea epibenthic fauna from the Balearic (NW Mediterranean) and Ionian (SE Mwediterranean) Basins.}, author = {W Plaiti and F Sardá and A Tselepides}, year = {2002}, date = {2002-01-01}, address = {Lodz, Poland.}, note = {Publication Title: The 4th European Crustacean Conference.}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Rigos, G; Tyrpenou, A; Nengas, I; Alexis, M A pharmacokinetic study of flumequine in sea bass, Dicentrarchus labrax (L.), after a single intravascular injection Journal Article Journal of Fish Diseases, 25 , pp. 101 – 105, 2002. @article{rigos_pharmacokinetic_2002, title = {A pharmacokinetic study of flumequine in sea bass, Dicentrarchus labrax (L.), after a single intravascular injection}, author = {G Rigos and A Tyrpenou and I Nengas and M Alexis}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0036179502&partnerID=40&md5=fd41e0c3449ea53a451c6d5064795226}, doi = {https://doi.org/10.1046/j.1365-2761.2002.00340.x}, year = {2002}, date = {2002-01-01}, journal = {Journal of Fish Diseases}, volume = {25}, pages = {101 -- 105}, abstract = {The pharmacokinetic properties of flumequine following a single intravascular injection (10 mg kg -1 fish) were studied in sea bass, Dicentrarchus labrax (L.), 120 g held at 18°C. The absorption half life (t 1/2α) and the elimination half life (t 1/2β) of the drug were calculated to be 1.05 and 10.71 h, respectively. Tissue penetration of flumequine seemed to be moderate because both the apparent volume of distribution of the drug at steady-state (V d(ss)) and the extensive apparent volume of the central compartment (V c) were found to be small (1.51 and 0.626 L kg -1). The mean residence time (MRT) was short (09.73 h) and the total clearance (CL T) of the drug was rapid (0.156 L kg -1 h -1).}, keywords = {}, pubstate = {published}, tppubtype = {article} } The pharmacokinetic properties of flumequine following a single intravascular injection (10 mg kg -1 fish) were studied in sea bass, Dicentrarchus labrax (L.), 120 g held at 18°C. The absorption half life (t 1/2α) and the elimination half life (t 1/2β) of the drug were calculated to be 1.05 and 10.71 h, respectively. Tissue penetration of flumequine seemed to be moderate because both the apparent volume of distribution of the drug at steady-state (V d(ss)) and the extensive apparent volume of the central compartment (V c) were found to be small (1.51 and 0.626 L kg -1). The mean residence time (MRT) was short (09.73 h) and the total clearance (CL T) of the drug was rapid (0.156 L kg -1 h -1). |
Cotou, E; Papathanassiou, E; Tsangaris, C Environmental Pollution, 119 , pp. 141 – 149, 2002. @article{cotou_assessing_2002, title = {Assessing the quality of marine coastal environments: Comparison of scope for growth and Microtox® bioassay results of pollution gradient areas in eastern Mediterranean (Greece)}, author = {E Cotou and E Papathanassiou and C Tsangaris}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0036009842&partnerID=40&md5=e98eb4f218cec741ee061ca438d806b2}, year = {2002}, date = {2002-01-01}, journal = {Environmental Pollution}, volume = {119}, pages = {141 -- 149}, abstract = {Methods for assessing the quality of marine coastal environments are numerous and rapidly evolving. The integration of physiological parameters termed as Scope for Growth (SfG) and the luminescent bioassay Microtox® were applied in several sites of Saronikos and Amvrakikos Gulfs (Greece) to assess the environmental quality and compare the results of the two methods. Each site in the two areas undergoes different types and levels of pollution. Both methods could identify a pollution gradient reflecting the quality of each site. A very good linear correlation was noticed between the two methods (r=0.98). The stress order, which was similar in both methods, exhibited the expected pollution gradient according to the pressure that each site undergoes. Results suggest that for screening and fast isolation of polluted sites before further analysis, the rapid and simple Microtox® assay could operate as an alternative to the more complex SfG method. © 2002 Elsevier Science Ltd. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Methods for assessing the quality of marine coastal environments are numerous and rapidly evolving. The integration of physiological parameters termed as Scope for Growth (SfG) and the luminescent bioassay Microtox® were applied in several sites of Saronikos and Amvrakikos Gulfs (Greece) to assess the environmental quality and compare the results of the two methods. Each site in the two areas undergoes different types and levels of pollution. Both methods could identify a pollution gradient reflecting the quality of each site. A very good linear correlation was noticed between the two methods (r=0.98). The stress order, which was similar in both methods, exhibited the expected pollution gradient according to the pressure that each site undergoes. Results suggest that for screening and fast isolation of polluted sites before further analysis, the rapid and simple Microtox® assay could operate as an alternative to the more complex SfG method. © 2002 Elsevier Science Ltd. All rights reserved. |
Garriques, P; Narbonne, J F; Budzinski, H; Viarengo, A; Lehtonen, K; Bocquene, G; Andersen, O K; Peters, L; Lowe, D; Cajaraville, M P; E.Cotou, ; Dimitriadis, V; Mnier, C; Porte, C; Auffret, M; Devillers, J; Hansen, P D; Fortlin, L; Koehler, A; Barsiene, J; Balk, L; Jenssen, B M; Savva, D; Rahmani, R; Schiedek, D; Vuorinen, P; Lang, T; Pempkowiak, J; Skorkowski, E; Yawetz, A; Gercken, J; Bolognesi, C; Depledge, M Biological effects of environmental pollution in coastal marine ecosystems: The BEEP Project Journal Article Marine Environmental Research, 54 , pp. 843–844, 2002. @article{garriques_biological_2002, title = {Biological effects of environmental pollution in coastal marine ecosystems: The BEEP Project}, author = {P Garriques and J F Narbonne and H Budzinski and A Viarengo and K Lehtonen and G Bocquene and O K Andersen and L Peters and D Lowe and M P Cajaraville and E.Cotou and V Dimitriadis and C Mnier and C Porte and M Auffret and J Devillers and P D Hansen and L Fortlin and A Koehler and J Barsiene and L Balk and B M Jenssen and D Savva and R Rahmani and D Schiedek and P Vuorinen and T Lang and J Pempkowiak and E Skorkowski and A Yawetz and J Gercken and C Bolognesi and M Depledge}, year = {2002}, date = {2002-01-01}, journal = {Marine Environmental Research}, volume = {54}, pages = {843--844}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Moreno, A; Pereira, J; Arvanitidis, C; Robin, J -P; Koutsoubas, D; Perales-Raya, C; Cunha, M M; Balguerias, E; Denis, V Biological variation of Loligo vulgaris (Cephalopoda: Loliginidae) in the eastern Atlantic and Mediterranean Journal Article Bulletin of Marine Science, 71 (1), pp. 515–534, 2002, ISSN: 00074977. @article{moreno_biological_2002, title = {Biological variation of Loligo vulgaris (Cephalopoda: Loliginidae) in the eastern Atlantic and Mediterranean}, author = {A Moreno and J Pereira and C Arvanitidis and J -P Robin and D Koutsoubas and C Perales-Raya and M M Cunha and E Balguerias and V Denis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0038414518&partnerID=40&md5=697c23d401ca449162a83e6eebe8c623}, issn = {00074977}, year = {2002}, date = {2002-01-01}, journal = {Bulletin of Marine Science}, volume = {71}, number = {1}, pages = {515--534}, abstract = {The biological characteristics of the squid Loligo vulgaris from north France, northwest Portugal, the Saharan Bank, and the Greek Seas were analyzed to describe large-scale biological patterns and to evaluate geographical variation in the Atlantic and the Mediterranean. In northwest Portugal and on the Saharan Bank population length structures are more complex due to extended spawning and recruitment periods. Squid spawn only between November and April in north France and the Greek Seas. Gonadosomatic indices decreased with decreasing latitude in the Atlantic, while the highest indices were found in the Mediterranean. Full maturity occurred at smaller size in northwest Portugal than in other areas of the Atlantic, and at similar size to Mediterranean squid. Length-weight relationship slopes increased from the north to the south in the Atlantic and in the Mediterranean. Multivariate analysis of seasonal biological indices demonstrated significant biological differences between squid of different areas, mainly in terms of size at maturity, male GSI and average body size and weight. Biological variability between areas was considered related to plasticity of responses to large-scale geographic environmental conditions.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The biological characteristics of the squid Loligo vulgaris from north France, northwest Portugal, the Saharan Bank, and the Greek Seas were analyzed to describe large-scale biological patterns and to evaluate geographical variation in the Atlantic and the Mediterranean. In northwest Portugal and on the Saharan Bank population length structures are more complex due to extended spawning and recruitment periods. Squid spawn only between November and April in north France and the Greek Seas. Gonadosomatic indices decreased with decreasing latitude in the Atlantic, while the highest indices were found in the Mediterranean. Full maturity occurred at smaller size in northwest Portugal than in other areas of the Atlantic, and at similar size to Mediterranean squid. Length-weight relationship slopes increased from the north to the south in the Atlantic and in the Mediterranean. Multivariate analysis of seasonal biological indices demonstrated significant biological differences between squid of different areas, mainly in terms of size at maturity, male GSI and average body size and weight. Biological variability between areas was considered related to plasticity of responses to large-scale geographic environmental conditions. |
Alasalvar, C; Taylor, K D A; Öksüz, A; Shahidi, F; Alexis, M Comparison of freshness quality of cultured and wild sea bass (Dicentrarchus labrax) Journal Article Journal of Food Science, 67 (9), pp. 3220–3226, 2002, ISSN: 0022-1147, 1750-3841. @article{alasalvar_comparison_2002, title = {Comparison of freshness quality of cultured and wild sea bass (Dicentrarchus labrax)}, author = {C Alasalvar and K D A Taylor and A Öksüz and F Shahidi and M Alexis}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0036867918&partnerID=40&md5=fcf5f519de82c4f649e587a9b1eb5055}, doi = {10.1111/j.1365-2621.2002.tb09569.x}, issn = {0022-1147, 1750-3841}, year = {2002}, date = {2002-01-01}, journal = {Journal of Food Science}, volume = {67}, number = {9}, pages = {3220--3226}, abstract = {Freshness quality of cultured and wild sea bass stored in ice for up to 22 d was evaluated by monitoring sensory quality, levels of nucleotides, and nucleotide breakdown products. Sensory schemes for raw and cooked fish were modified according to the trained panelists’ perceptions, during ice storage. Freshness K and related values, namely K, Ki, G, P, H, and Fr, were calculated. The limit for acceptability of cultured and wild sea bass stored in ice was approximately 16 to 18 d. The sensory score for both cultured and wild raw fish was correlated with some freshness indicators (K, Ki, G, P, and Fr) values over the entire storage period (r2 values textgreater= 0.98).}, keywords = {}, pubstate = {published}, tppubtype = {article} } Freshness quality of cultured and wild sea bass stored in ice for up to 22 d was evaluated by monitoring sensory quality, levels of nucleotides, and nucleotide breakdown products. Sensory schemes for raw and cooked fish were modified according to the trained panelists’ perceptions, during ice storage. Freshness K and related values, namely K, Ki, G, P, H, and Fr, were calculated. The limit for acceptability of cultured and wild sea bass stored in ice was approximately 16 to 18 d. The sensory score for both cultured and wild raw fish was correlated with some freshness indicators (K, Ki, G, P, and Fr) values over the entire storage period (r2 values textgreater= 0.98). |
Grigorakis, K; Alexis, M N; Taylor, Anthony K D; Hole, M Comparison of wild and cultured gilthead sea bream (Sparus aurata); composition, appearance and seasonal variations Journal Article International Journal of Food Science and Technology, 37 (5), pp. 477–484, 2002. @article{grigorakis_comparison_2002, title = {Comparison of wild and cultured gilthead sea bream (Sparus aurata); composition, appearance and seasonal variations}, author = {K Grigorakis and M N Alexis and K D Anthony Taylor and M Hole}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0036072021&partnerID=40&md5=b91b887e4e6b84e0a210b498bc49857e}, doi = {https://doi.org/10.1046/j.1365-2621.2002.00604.x}, year = {2002}, date = {2002-01-01}, journal = {International Journal of Food Science and Technology}, volume = {37}, number = {5}, pages = {477--484}, abstract = {Major quality parameters, such as muscle composition, fat deposition, muscle fatty acid composition and external appearance were studied in wild and cultured gilthead sea bream. Muscle fat content and total depot fat (peritoneal and perivisceral fat) indicated a seasonal variation with minimum values observed in late spring and maximum in late summer. Gonadosomatic indices of cultured fish were lower than those found in wild specimens. Lipid content of cultured sea bream was much higher than that of wild fish. Differences were also observed in fatty acid profiles. Cultured fish were characterized by higher levels of monoenes, n-9 and 18:2n-6 fatty acids and wild fish by higher levels of saturates, 20:4n-6, n-3 fatty acids and n-3/n-6 ratios. Differences were also noted in the external appearance of fish.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Major quality parameters, such as muscle composition, fat deposition, muscle fatty acid composition and external appearance were studied in wild and cultured gilthead sea bream. Muscle fat content and total depot fat (peritoneal and perivisceral fat) indicated a seasonal variation with minimum values observed in late spring and maximum in late summer. Gonadosomatic indices of cultured fish were lower than those found in wild specimens. Lipid content of cultured sea bream was much higher than that of wild fish. Differences were also observed in fatty acid profiles. Cultured fish were characterized by higher levels of monoenes, n-9 and 18:2n-6 fatty acids and wild fish by higher levels of saturates, 20:4n-6, n-3 fatty acids and n-3/n-6 ratios. Differences were also noted in the external appearance of fish. |
Berrebi, P; Tsigenopoulos, C S Contribution des sequences mitochondriales a l’eclatement phylogenetique du genre polyploide Barbus (Teleosteen Cyprinides). Implications biogeographiques Inproceedings Journées Annuelles de la Société Française de Systématique (SFS) ’Systématique et biogéographie’, pp. 49–56, Biosystema, Paris, France, 2002, (Backup Publisher: Biosystema). @inproceedings{berrebi_contribution_2002, title = {Contribution des sequences mitochondriales a l’eclatement phylogenetique du genre polyploide Barbus (Teleosteen Cyprinides). Implications biogeographiques}, author = {P Berrebi and C S Tsigenopoulos}, year = {2002}, date = {2002-01-01}, booktitle = {Journées Annuelles de la Société Française de Systématique (SFS) ’Systématique et biogéographie’}, volume = {20}, pages = {49--56}, publisher = {Biosystema}, address = {Paris, France}, note = {Backup Publisher: Biosystema}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Tyrpenou, A E; Rigos, G G; Athanassopoulou, F Determination of chloramphenicol residues in gilthead seabream (Sparus aurata L.) tissues by HPLC-PDA Journal Article Journal of Liquid Chromatography and Related Technologies, 25 , pp. 655 – 663, 2002. @article{tyrpenou_determination_2002, title = {Determination of chloramphenicol residues in gilthead seabream (Sparus aurata L.) tissues by HPLC-PDA}, author = {A E Tyrpenou and G G Rigos and F Athanassopoulou}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0036123272&partnerID=40&md5=888db851499e8a6aaced859b08a724b5}, year = {2002}, date = {2002-01-01}, journal = {Journal of Liquid Chromatography and Related Technologies}, volume = {25}, pages = {655 -- 663}, abstract = {A high performance liquid chromatographic method for the determination of chloramphenicol residues in muscle tissue of the cultured fish gilthead seabream (Sparus aurata L.) was developed. Chloramphenicol is extracted with ethyl acetate and after centrifugation and solvent evaporation the oily extract is partitioned between 3% sodium chloride solution and n-pentane, and chloramphenicol is extracted back into ethyl acetate. After evaporation to near dryness, the residue is dissolved in n-hexane and is cleaned up on a Silica gel SPE mini column. Chloramphenicol was analyzed on a ZORBAX SB - C18 column at a temperature of 50°C, with the mobile phase being methanol:-water 30 + 70 v/v delivered isocratically. Detection was performed using a Photo Diode Array detector monitored at λmax ∼278 nm. The mean recovery (R%) achieved was 88.624 ± 9.65% for a range of 10, 25, 50, 100, 200 μg/kg blank fortified samples (n = 4). The limit of detection (LOD) was 1.87 ng corresponding to 5 μg/kg chloramphenicol in muscle and the limit of quantification (LOQ) was 10 μg/kg.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A high performance liquid chromatographic method for the determination of chloramphenicol residues in muscle tissue of the cultured fish gilthead seabream (Sparus aurata L.) was developed. Chloramphenicol is extracted with ethyl acetate and after centrifugation and solvent evaporation the oily extract is partitioned between 3% sodium chloride solution and n-pentane, and chloramphenicol is extracted back into ethyl acetate. After evaporation to near dryness, the residue is dissolved in n-hexane and is cleaned up on a Silica gel SPE mini column. Chloramphenicol was analyzed on a ZORBAX SB - C18 column at a temperature of 50°C, with the mobile phase being methanol:-water 30 + 70 v/v delivered isocratically. Detection was performed using a Photo Diode Array detector monitored at λmax ∼278 nm. The mean recovery (R%) achieved was 88.624 ± 9.65% for a range of 10, 25, 50, 100, 200 μg/kg blank fortified samples (n = 4). The limit of detection (LOD) was 1.87 ng corresponding to 5 μg/kg chloramphenicol in muscle and the limit of quantification (LOQ) was 10 μg/kg. |
Valavanis, V D; Georgakarakos, S; Koutsoubas, D; Arvanitidis, C; Haralabous, J Development of a marine information system for cephalopod fisheries in Eastern Mediterranean Journal Article Bulletin of Marine Science, 71 (2), pp. 867–882, 2002, ISSN: 00074977. @article{valavanis_development_2002, title = {Development of a marine information system for cephalopod fisheries in Eastern Mediterranean}, author = {V D Valavanis and S Georgakarakos and D Koutsoubas and C Arvanitidis and J Haralabous}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037847554&partnerID=40&md5=58a9f1fdcd43728aa0d3c54aa022065c}, issn = {00074977}, year = {2002}, date = {2002-01-01}, journal = {Bulletin of Marine Science}, volume = {71}, number = {2}, pages = {867--882}, abstract = {An interfaced marine information system is developed for integrated analysis of fisheries of five commercially important cephalopod species in Greek waters of the Eastern Mediterranean. The system combines data on the spatial and temporal patterns of cephalopod population dynamics focusing on geo-distribution of abundance, environmental variation, fisheries, spawning areas and migration habits. The system is developed as a customisation of a workstation ARC/INFO environment and features a series of innovative GIS map-overlay and integration routines for analysis and modelling of surveyed, statistical, and remote-sensed data. Geo-referenced datasets include cephalopod catch and landings, coastline-bathymetry, bottom substrate types, and a set of environmental variables provided by satellite sensors (AVHRR/sea surface temperature and SeaWiFS/chlorophyll-a concentration) and climatologic datasets (sea surface salinity). The innovative aspect of this marine system is the integration of species life history data to GIS analysis. Species preferences on certain spawning conditions, migration habits, and depth ranges are used as constraints in GIS analysis and integration. The application of GIS and Remote Sensing technologies has proved useful for the mapping of seasonal spatial components of cephalopod population dynamics. Results from this application may be used for information-based species management proposals, which is the goal of further development of this marine information system.}, keywords = {}, pubstate = {published}, tppubtype = {article} } An interfaced marine information system is developed for integrated analysis of fisheries of five commercially important cephalopod species in Greek waters of the Eastern Mediterranean. The system combines data on the spatial and temporal patterns of cephalopod population dynamics focusing on geo-distribution of abundance, environmental variation, fisheries, spawning areas and migration habits. The system is developed as a customisation of a workstation ARC/INFO environment and features a series of innovative GIS map-overlay and integration routines for analysis and modelling of surveyed, statistical, and remote-sensed data. Geo-referenced datasets include cephalopod catch and landings, coastline-bathymetry, bottom substrate types, and a set of environmental variables provided by satellite sensors (AVHRR/sea surface temperature and SeaWiFS/chlorophyll-a concentration) and climatologic datasets (sea surface salinity). The innovative aspect of this marine system is the integration of species life history data to GIS analysis. Species preferences on certain spawning conditions, migration habits, and depth ranges are used as constraints in GIS analysis and integration. The application of GIS and Remote Sensing technologies has proved useful for the mapping of seasonal spatial components of cephalopod population dynamics. Results from this application may be used for information-based species management proposals, which is the goal of further development of this marine information system. |
Papandroulakis, N; Divanach, P; Kentouri, M Enhanced biological performance of intensive sea bream (Sparus aurata) larviculture in the presence of phytoplankton with long photophase Journal Article Aquaculture, 204 , pp. 45 – 63, 2002. @article{papandroulakis_enhanced_2002, title = {Enhanced biological performance of intensive sea bream (Sparus aurata) larviculture in the presence of phytoplankton with long photophase}, author = {N Papandroulakis and P Divanach and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0037148117&partnerID=40&md5=05a492b10b2b9875a20edf9b69f585b8}, year = {2002}, date = {2002-01-01}, journal = {Aquaculture}, volume = {204}, pages = {45 -- 63}, abstract = {Sea bream larvae were reared under intensive conditions either with (pseudo-green water technique) or without (clear water technique) the addition of phytoplankton in the rearing tanks, under 24- or 18-h photophases. Phytoplankton presence in the rearing medium resulted in 44 ± 17% survival and individuals of 2.0 ± 0.2 mg wet weight after 20 days of rearing. With the clear water method, both survival and growth decreased to 16 ± 6% and 1.1 ± 0.2 mg, respectively. The daily consumption rate (as milligram of carbon consumed per milligram body carbon) at the beginning of exogenous feeding was 0.5 day-1 for all experimental conditions. At later stages, individuals reared with phytoplankton present had a daily consumption rate of about 0.7 day-1, while those reared with the clear water technique presented consumption rates from 1.0 (for 24-h photophase) to 1.9 day-1 (for 18-h photophase). The mean food assimilation efficiency of the larvae, expressed as a Food Conversion Index (FCI), was between 6.3 ± 1.4 (for 18-h photophase) and 8.2 ± 1.2 (for 24-h photophase) for the individuals reared in the presence of phytoplankton. For larvae reared using the clear water technique, FCI was between 12.8 ± 2.4 (for 24-h photophase) and 20.1 ± 5.3 (for 18-h photophase). © 2002 Elsevier Science B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Sea bream larvae were reared under intensive conditions either with (pseudo-green water technique) or without (clear water technique) the addition of phytoplankton in the rearing tanks, under 24- or 18-h photophases. Phytoplankton presence in the rearing medium resulted in 44 ± 17% survival and individuals of 2.0 ± 0.2 mg wet weight after 20 days of rearing. With the clear water method, both survival and growth decreased to 16 ± 6% and 1.1 ± 0.2 mg, respectively. The daily consumption rate (as milligram of carbon consumed per milligram body carbon) at the beginning of exogenous feeding was 0.5 day-1 for all experimental conditions. At later stages, individuals reared with phytoplankton present had a daily consumption rate of about 0.7 day-1, while those reared with the clear water technique presented consumption rates from 1.0 (for 24-h photophase) to 1.9 day-1 (for 18-h photophase). The mean food assimilation efficiency of the larvae, expressed as a Food Conversion Index (FCI), was between 6.3 ± 1.4 (for 18-h photophase) and 8.2 ± 1.2 (for 24-h photophase) for the individuals reared in the presence of phytoplankton. For larvae reared using the clear water technique, FCI was between 12.8 ± 2.4 (for 24-h photophase) and 20.1 ± 5.3 (for 18-h photophase). © 2002 Elsevier Science B.V. All rights reserved. |
Koutandos, E; Karambas, T; Koutitas, C; Prinos, P Floating breakwaters efficiency in intermediate and shallow waters Inproceedings pp. 177–178, 2002, (Publication Title: International Conference ‘Hydroscience-2002’). @inproceedings{koutandos_floating_2002, title = {Floating breakwaters efficiency in intermediate and shallow waters}, author = {E Koutandos and T Karambas and C Koutitas and P Prinos}, year = {2002}, date = {2002-01-01}, volume = {1}, pages = {177--178}, note = {Publication Title: International Conference ‘Hydroscience-2002’}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Mandalakis, M; Tsapakis, M; Tsoga, A; Stephanou, E G Gas-particle concentrations and distribution of aliphatic hydrocarbons, PAHs, PCBs and PCDD/Fs in the atmosphere of Athens (Greece) Journal Article Atmospheric Environment, 36 (25), pp. 4023–4035, 2002, ISSN: 13522310. @article{mandalakis_gas-particle_2002, title = {Gas-particle concentrations and distribution of aliphatic hydrocarbons, PAHs, PCBs and PCDD/Fs in the atmosphere of Athens (Greece)}, author = {M Mandalakis and M Tsapakis and A Tsoga and E G Stephanou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036721043&doi=10.1016%2fS1352-2310%2802%2900362-X&partnerID=40&md5=161204708dab3e6a7e4e7908d8e4d22d}, doi = {10.1016/S1352-2310(02)00362-X}, issn = {13522310}, year = {2002}, date = {2002-01-01}, journal = {Atmospheric Environment}, volume = {36}, number = {25}, pages = {4023--4035}, abstract = {Air samples were collected at the urban center, a background site, and the adjacent coastal area of the metropolitan area of Athens during July 2000. Gas and particle phase concentrations of aliphatic hydrocarbons, α,β-hopanes, polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and 2,3,7,8-substituted polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) were identified and measured. Total concentrations of aliphatic hydrocarbons, ranged between 943.0ngm-3 (urban site) and 947.1ngm-3 (coastal area) in the gas and from 141.8ngm-3 (coastal site) up to 469.0ngm-3 (urban center) in the particulate phase. The total concentration of α,β-hopanes (12 homologues) in the urban area was ca. 0.2ngm-3 in the gas and 1.0ngm-3 in the particulate phase. Total PAH concentrations (∑PAHs of 20 members) ranged from 3.5ngm-3 (background site) to 26.0ngm-3 (urban center) in the gas and from 1.3ngm-3 (coastal area) up to 5.0ngm-3 (urban center) in the particulate phase. The total concentration of PCBs (∑PCBs of 38 congeners) in the gas plus the particulate phase of the atmosphere ranged from 80.5pgm-3 (background site) up to 348.6pgm-3 (urban center) and the corresponding concentration of PCDD/Fs ranged from 166.6fgm-3 (background site) up to 701.5fgm-3 (urban center), respectively. Gas-particle partition coefficients Kp of n-alkanes, PAHs, PCBs and PCDD/Fs were well correlated (R2=0.50-0.99) with the sub-cooled liquid vapor pressure PL o. The average slopes mr of logKp versus log PL o for n-alkanes (-0.46) and PCBs (-0.44) were low compared to other urban areas. The corresponding mr values for PAHs (-0.57), PCDDs (-0.78) and PCDFs (-0.75) are comparable to those obtained in other urban areas. Copyright © 2002 Elsevier Science Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Air samples were collected at the urban center, a background site, and the adjacent coastal area of the metropolitan area of Athens during July 2000. Gas and particle phase concentrations of aliphatic hydrocarbons, α,β-hopanes, polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and 2,3,7,8-substituted polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) were identified and measured. Total concentrations of aliphatic hydrocarbons, ranged between 943.0ngm-3 (urban site) and 947.1ngm-3 (coastal area) in the gas and from 141.8ngm-3 (coastal site) up to 469.0ngm-3 (urban center) in the particulate phase. The total concentration of α,β-hopanes (12 homologues) in the urban area was ca. 0.2ngm-3 in the gas and 1.0ngm-3 in the particulate phase. Total PAH concentrations (∑PAHs of 20 members) ranged from 3.5ngm-3 (background site) to 26.0ngm-3 (urban center) in the gas and from 1.3ngm-3 (coastal area) up to 5.0ngm-3 (urban center) in the particulate phase. The total concentration of PCBs (∑PCBs of 38 congeners) in the gas plus the particulate phase of the atmosphere ranged from 80.5pgm-3 (background site) up to 348.6pgm-3 (urban center) and the corresponding concentration of PCDD/Fs ranged from 166.6fgm-3 (background site) up to 701.5fgm-3 (urban center), respectively. Gas-particle partition coefficients Kp of n-alkanes, PAHs, PCBs and PCDD/Fs were well correlated (R2=0.50-0.99) with the sub-cooled liquid vapor pressure PL o. The average slopes mr of logKp versus log PL o for n-alkanes (-0.46) and PCBs (-0.44) were low compared to other urban areas. The corresponding mr values for PAHs (-0.57), PCDDs (-0.78) and PCDFs (-0.75) are comparable to those obtained in other urban areas. Copyright © 2002 Elsevier Science Ltd. |
Navajas, M; Perrot-Minnot, M J; Lagnel, J; Migeon, A; Bourse, T; Cornuet, J M Genetic structure of a greenhouse population of the spider mite Tetranychus urticae: Spatio-temporal analysis with microsatellite markers Journal Article Insect Molecular Biology, 11 , pp. 157 – 165, 2002. @article{navajas_genetic_2002, title = {Genetic structure of a greenhouse population of the spider mite Tetranychus urticae: Spatio-temporal analysis with microsatellite markers}, author = {M Navajas and M J Perrot-Minnot and J Lagnel and A Migeon and T Bourse and J M Cornuet}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0036010997&partnerID=40&md5=6194a6b3f51d8c4d5dfa9d3e45c9c9e2}, year = {2002}, date = {2002-01-01}, journal = {Insect Molecular Biology}, volume = {11}, pages = {157 -- 165}, abstract = {The genetic structure of a greenhouse population of the mite Tetranychus urticae was studied by the analysis of five microsatellite loci. Genetic variation was compared during a crop season between periods of population foundation and rapid population increase and was investigated in two consecutive years. The population displayed significant heterozygote deficiency at all the sampling periods. However, inbreeding tended to decrease with increasing density (FIS coefficient between 0.13 and 0.25). No significant genetic differentiation between samples was found either at a spatial scale within the greenhouse or at a temporal scale between two growing seasons (FST between 0.008 and 0.09). Estimations of the genetic relatedness between pairs of individuals indicated that the distances between pairs of sisters and unrelated mites in the greenhouse were not significantly different, suggesting that mites do not tend to form patches that reside close to the point of birth.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The genetic structure of a greenhouse population of the mite Tetranychus urticae was studied by the analysis of five microsatellite loci. Genetic variation was compared during a crop season between periods of population foundation and rapid population increase and was investigated in two consecutive years. The population displayed significant heterozygote deficiency at all the sampling periods. However, inbreeding tended to decrease with increasing density (FIS coefficient between 0.13 and 0.25). No significant genetic differentiation between samples was found either at a spatial scale within the greenhouse or at a temporal scale between two growing seasons (FST between 0.008 and 0.09). Estimations of the genetic relatedness between pairs of individuals indicated that the distances between pairs of sisters and unrelated mites in the greenhouse were not significantly different, suggesting that mites do not tend to form patches that reside close to the point of birth. |
Gustafsson, Ö; Axelman, J; Bandh, C; Jönsson, A; Broman, D; Breivik, K; Manö, S; Pacyna, J; Cziudaj, G; Petrick, G; Schulz-Bull, D; Meijer, S; Ockenden, W; Jones, K; Sweetman, A; Mandalakis, M; Tsapakis, M; Stephanou, E G; van Drooge, B; Ribes, S; Torres, C; Grimalt, J Global budget for PCBs provides prediction of their environmental longevities Inproceedings Abstracts of Papers of the American Chemical Society, pp. Part 1–2, ENVR 7, 2002, (Publication Title: Abstracts of Papers of the American Chemical Society Type: Conference Publication Part 1-2, ENVR 7). @inproceedings{gustafsson_global_2002, title = {Global budget for PCBs provides prediction of their environmental longevities}, author = {Ö Gustafsson and J Axelman and C Bandh and A Jönsson and D Broman and K Breivik and S Manö and J Pacyna and G Cziudaj and G Petrick and D Schulz-Bull and S Meijer and W Ockenden and K Jones and A Sweetman and M Mandalakis and M Tsapakis and E G Stephanou and B van Drooge and S Ribes and C Torres and J Grimalt}, year = {2002}, date = {2002-01-01}, booktitle = {Abstracts of Papers of the American Chemical Society}, volume = {224}, pages = {Part 1--2, ENVR 7}, note = {Publication Title: Abstracts of Papers of the American Chemical Society Type: Conference Publication Part 1-2, ENVR 7}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Garnier-Géré, P H; Naciri-Graven, Y; Bougrier, S; Magoulas, A; Héral, M; Kotoulas, G; Hawkins, A; Gérard, A Molecular Ecology, 11 (8), pp. 1499–1514, 2002, ISSN: 09621083. @article{garnier-gere_influences_2002, title = {Influences of triploidy, parentage and genetic diversity on growth of the Pacific oyster Crassostrea gigas reared in contrasting natural environments}, author = {P H Garnier-Géré and Y Naciri-Graven and S Bougrier and A Magoulas and M Héral and G Kotoulas and A Hawkins and A Gérard}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035993750&doi=10.1046%2fj.1365-294X.2002.01531.x&partnerID=40&md5=fda8b0e20f8e19d34fe231f8cfaf81a2}, doi = {10.1046/j.1365-294X.2002.01531.x}, issn = {09621083}, year = {2002}, date = {2002-01-01}, journal = {Molecular Ecology}, volume = {11}, number = {8}, pages = {1499--1514}, abstract = {An increasing number of hypotheses are being proposed to explain the faster growth potential of triploids in molluscs, including their partial sterility or their higher heterozygosity compared to diploids. Triploid advantage however, remains controversial for poorer sites, because of a potential trade-off with survival. These questions were addressed in Crassostrea gigas by deploying meiosis II triploids and their diploid siblings from a single mass spawning of three males and seven females, in two contrasting locations for their trophic resources. One hundred and fifty individuals were sampled at each site after nine months, measured for weight and biochemical composition, and genotyped using three microsatellite and seven allozyme loci. Higher performance was observed at the fastgrowing site for all traits except shell weight, and triploids had greater weights and biochemical contents than diploids at harvest. Triploids also grew faster at the poorer site, and showed similar survival rates to diploids at both sites. Triploids had significantly higher average allozyme and microsatellite diversity. However, they performed better for a wide range of individual heterozygosity values, arguing for an advantage of the triploid state per se, that could be due to positive effects on growth of both sterility of triploids with subsequent resource re-allocation and possible faster transcription with three copies of each gene. Despite evidence of very low or no inbreeding in the diploid sample, positive associations between individual allozyme diversity and growth were detected, which explained little but significant amounts of phenotypic variation. These associations were interpreted as direct effects of allozymes, either alone or including epistatic interactions with other loci. In addition, measures of individual distance (mean-d2) specific to microsatellites, were negatively correlated with growth in diploids, indicating possible effects of outbreeding depression between more distant genomes of parents from distinct populations.}, keywords = {}, pubstate = {published}, tppubtype = {article} } An increasing number of hypotheses are being proposed to explain the faster growth potential of triploids in molluscs, including their partial sterility or their higher heterozygosity compared to diploids. Triploid advantage however, remains controversial for poorer sites, because of a potential trade-off with survival. These questions were addressed in Crassostrea gigas by deploying meiosis II triploids and their diploid siblings from a single mass spawning of three males and seven females, in two contrasting locations for their trophic resources. One hundred and fifty individuals were sampled at each site after nine months, measured for weight and biochemical composition, and genotyped using three microsatellite and seven allozyme loci. Higher performance was observed at the fastgrowing site for all traits except shell weight, and triploids had greater weights and biochemical contents than diploids at harvest. Triploids also grew faster at the poorer site, and showed similar survival rates to diploids at both sites. Triploids had significantly higher average allozyme and microsatellite diversity. However, they performed better for a wide range of individual heterozygosity values, arguing for an advantage of the triploid state per se, that could be due to positive effects on growth of both sterility of triploids with subsequent resource re-allocation and possible faster transcription with three copies of each gene. Despite evidence of very low or no inbreeding in the diploid sample, positive associations between individual allozyme diversity and growth were detected, which explained little but significant amounts of phenotypic variation. These associations were interpreted as direct effects of allozymes, either alone or including epistatic interactions with other loci. In addition, measures of individual distance (mean-d2) specific to microsatellites, were negatively correlated with growth in diploids, indicating possible effects of outbreeding depression between more distant genomes of parents from distinct populations. |
Georgakarakos, S; Haralabous, J; Valavanis, V; Arvanitidis, C; Koutsoubas, D; Kapantagakis, A Loliginid and ommastrephid stock prediction in greek waters using time series analysis techniques Journal Article Bulletin of Marine Science, 71 (1), pp. 269–287, 2002, ISSN: 00074977. @article{georgakarakos_loliginid_2002, title = {Loliginid and ommastrephid stock prediction in greek waters using time series analysis techniques}, author = {S Georgakarakos and J Haralabous and V Valavanis and C Arvanitidis and D Koutsoubas and A Kapantagakis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0012558157&partnerID=40&md5=ba8fe464323fefdcf7bb74558465a3f4}, issn = {00074977}, year = {2002}, date = {2002-01-01}, journal = {Bulletin of Marine Science}, volume = {71}, number = {1}, pages = {269--287}, abstract = {Time series of loliginid and ommastrephid landings were analysed taking into account spatio-temporal descriptors of sea surface temperature (SST). The data are based on fisheries statistics recorded from the three most important fishing ports in the Northern Aegean Sea (1984-1999) and NOAA satellite images processed using GIS and image analysis tools. Autocorrelation (AC) and partial autocorrelation (PAC) functions were estimated leading to the identification and construction of seasonal ARIMA models, suitable for explaining the time series and forecasting future abundance values. The performance of the models was tested by comparing the predicted against the observed data of the last year (1999) and by examining the distribution and the AC of the residuals. The analysis provided results characterizing the different fishing patterns in each geographic area, as well as new series containing seasonally adjusted values, trend, cycle and error components of the model. Time series of several statistical parameters describing spatio-temporal variations of the S ST were estimated and analysed aiming at the detection of anomalies and possible stock-environment relationships. Cross-correlation analysis between SST parameters and stock biomass indexes showed significant correlation coefficients, before and after compensation of the seasonal fluctuations by seasonal differencing. The results suggest that SST can be a leading indicator for stock prediction of the target species in the survey area.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Time series of loliginid and ommastrephid landings were analysed taking into account spatio-temporal descriptors of sea surface temperature (SST). The data are based on fisheries statistics recorded from the three most important fishing ports in the Northern Aegean Sea (1984-1999) and NOAA satellite images processed using GIS and image analysis tools. Autocorrelation (AC) and partial autocorrelation (PAC) functions were estimated leading to the identification and construction of seasonal ARIMA models, suitable for explaining the time series and forecasting future abundance values. The performance of the models was tested by comparing the predicted against the observed data of the last year (1999) and by examining the distribution and the AC of the residuals. The analysis provided results characterizing the different fishing patterns in each geographic area, as well as new series containing seasonally adjusted values, trend, cycle and error components of the model. Time series of several statistical parameters describing spatio-temporal variations of the S ST were estimated and analysed aiming at the detection of anomalies and possible stock-environment relationships. Cross-correlation analysis between SST parameters and stock biomass indexes showed significant correlation coefficients, before and after compensation of the seasonal fluctuations by seasonal differencing. The results suggest that SST can be a leading indicator for stock prediction of the target species in the survey area. |
Mañanós, E; Carrillo, M; Sorbera, L A; Mylonas, C C; Asturiano, J F; Bayarri, M J; Zohar, Y; Zanuy, S Journal of Fish Biology, 60 (2), pp. 328–339, 2002, ISSN: 00221112. @article{mananos_luteinizing_2002, title = {Luteinizing hormone and sexual steroid plasma levels after treatment of European sea bass with sustained-release delivery systems for gonadotropin-releasing hormone analogue}, author = {E Mañanós and M Carrillo and L A Sorbera and C C Mylonas and J F Asturiano and M J Bayarri and Y Zohar and S Zanuy}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036464151&doi=10.1006%2fjfbi.2001.1839&partnerID=40&md5=6f51e3fd5612a507457f3a7cfecdc891}, doi = {10.1006/jfbi.2001.1839}, issn = {00221112}, year = {2002}, date = {2002-01-01}, journal = {Journal of Fish Biology}, volume = {60}, number = {2}, pages = {328--339}, abstract = {Spermiating male European sea bass Dicentrarchus labrax were treated with gonadotropin-releasing hormone agonists (GnRHa), either a GnRHa injection (IN; 25 μg kg-1 body mass) or one of three types of controlled-release GnRHa-delivery systems: fast release implants (EVAc; 100 μg kg-1), slow release implants (EVSL; 100 μg kg-1) and slow release microspheres (MC; 50 μg kg-1). Luteinizing hormone (LH) release was highly stimulated by all GnRHa treatments, with elevated plasma levels lasting for 2 days in injected fish (IN) and 2, 4 and 6 weeks in controlled-release-treated fish (EVAc, MC and EVSL, respectively), correlating with a 1, 3, 5 and 5 week period of stimulation of milt production, respectively. Plasma levels of the androgens testosterone (T) and 11-ketotestosterone (11-KT), were not significantly affected by the GnRHa treatments. Plasma T was high at early spermiation and declined sharply near the end of this period. Plasma 11-KT levels declined continuously throughout the experiment. Levels of 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P), a proposed maturation-inducing steroid (MIS) in European sea bass, fluctuated around 0.2-1 ng ml-1 and were not greatly affected by the treatments. These results indicated a close correlation between sustained stimulation of LH release, achieved by GnRHa-delivery systems, and long-term enhancement of milt production. They also show an absence of changes in the common sex steroids, associated with elevated LH and enhanced spermiation. © 2002 The Fisheries Society of the British Isles.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Spermiating male European sea bass Dicentrarchus labrax were treated with gonadotropin-releasing hormone agonists (GnRHa), either a GnRHa injection (IN; 25 μg kg-1 body mass) or one of three types of controlled-release GnRHa-delivery systems: fast release implants (EVAc; 100 μg kg-1), slow release implants (EVSL; 100 μg kg-1) and slow release microspheres (MC; 50 μg kg-1). Luteinizing hormone (LH) release was highly stimulated by all GnRHa treatments, with elevated plasma levels lasting for 2 days in injected fish (IN) and 2, 4 and 6 weeks in controlled-release-treated fish (EVAc, MC and EVSL, respectively), correlating with a 1, 3, 5 and 5 week period of stimulation of milt production, respectively. Plasma levels of the androgens testosterone (T) and 11-ketotestosterone (11-KT), were not significantly affected by the GnRHa treatments. Plasma T was high at early spermiation and declined sharply near the end of this period. Plasma 11-KT levels declined continuously throughout the experiment. Levels of 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P), a proposed maturation-inducing steroid (MIS) in European sea bass, fluctuated around 0.2-1 ng ml-1 and were not greatly affected by the treatments. These results indicated a close correlation between sustained stimulation of LH release, achieved by GnRHa-delivery systems, and long-term enhancement of milt production. They also show an absence of changes in the common sex steroids, associated with elevated LH and enhanced spermiation. © 2002 The Fisheries Society of the British Isles. |
Potkonjak, V; Radojicic, J; Tzafestas, S Modeling robot "psycho-physical" state and reactions - A new option in human-robot communication part 1: Concept and background Journal Article Journal of Intelligent and Robotic Systems: Theory and Applications, 35 , pp. 339 – 352, 2002. @article{potkonjak_modeling_2002-1, title = {Modeling robot "psycho-physical" state and reactions - A new option in human-robot communication part 1: Concept and background}, author = {V Potkonjak and J Radojicic and S Tzafestas}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0036949985&partnerID=40&md5=37ee03a555f18e4b518474fbbb72e240}, year = {2002}, date = {2002-01-01}, journal = {Journal of Intelligent and Robotic Systems: Theory and Applications}, volume = {35}, pages = {339 -- 352}, abstract = {The concept and the theoretical background of the robot psycho-physical states were presented. A robot control scheme was elaborated to feature human-like behaviour to naturally resemble the motion of fatigued humans. The chemical based conditional reactions of human psycho-physical states like fear, stress and fatigue were studied.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The concept and the theoretical background of the robot psycho-physical states were presented. A robot control scheme was elaborated to feature human-like behaviour to naturally resemble the motion of fatigued humans. The chemical based conditional reactions of human psycho-physical states like fear, stress and fatigue were studied. |
Potkonjak, V; Radojicic, J; Tzafestas, S; Kostic, D Modeling robot "psycho-physical" state and reactions - A new option in human-robot communication part 2: Modeling and simulation Journal Article Journal of Intelligent and Robotic Systems: Theory and Applications, 35 , pp. 353 – 364, 2002. @article{potkonjak_modeling_2002, title = {Modeling robot "psycho-physical" state and reactions - A new option in human-robot communication part 2: Modeling and simulation}, author = {V Potkonjak and J Radojicic and S Tzafestas and D Kostic}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0036944969&partnerID=40&md5=e8279bc1eb58ca61ede449cb49241f43}, year = {2002}, date = {2002-01-01}, journal = {Journal of Intelligent and Robotic Systems: Theory and Applications}, volume = {35}, pages = {353 -- 364}, abstract = {This part of the paper examines numerically the possibility of modeling "robot fatigue’’ being representative of a human psychophysical state that can be applied to robots. Temperatures of driving motors are suggested as analogs to fatigue in muscles. Simulation of robot behavior is performed on a typical human task, namely handwriting. Three phases of task execution, characteristic for humans, are observed, i.e. regular motion, reconfiguration after symptoms of fatigue, and degeneration caused by the too long, hard work.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This part of the paper examines numerically the possibility of modeling "robot fatigue’’ being representative of a human psychophysical state that can be applied to robots. Temperatures of driving motors are suggested as analogs to fatigue in muscles. Simulation of robot behavior is performed on a typical human task, namely handwriting. Three phases of task execution, characteristic for humans, are observed, i.e. regular motion, reconfiguration after symptoms of fatigue, and degeneration caused by the too long, hard work. |
Petihakis, G; Triantafyllou, G; Allen, I J; Hoteit, I; Dounas, C Modelling the spatial and temporal variability of the Cretan Sea ecosystem Journal Article Journal of Marine Systems, 36 (3-4), pp. 173–196, 2002, ISSN: 09247963. @article{petihakis_modelling_2002, title = {Modelling the spatial and temporal variability of the Cretan Sea ecosystem}, author = {G Petihakis and G Triantafyllou and I J Allen and I Hoteit and C Dounas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036775648&doi=10.1016%2fS0924-7963%2802%2900186-0&partnerID=40&md5=f1e748d334e8ea4c11407d5a087e6585}, doi = {10.1016/S0924-7963(02)00186-0}, issn = {09247963}, year = {2002}, date = {2002-01-01}, journal = {Journal of Marine Systems}, volume = {36}, number = {3-4}, pages = {173--196}, abstract = {The ecosystem function of the oligotrophic Cretan Sea is explored through the development and application of a 3D ecological model. The simulation system comprises of two on-line coupled submodels: the 3D Princeton Ocean Model (POM) and the 1D European Regional Seas Ecosystem Model (ERSEM) adapted to the Cretan Sea. For the tuning and initialisation of the ecosystem parameters, the 1D version of the biogeochemical model is used. After a model spin up period of 10 years to reach a quasi-steady state, the results from an annual simulation are presented. A cost function is used as validation method for the comparison of model results with field data. The estimated annual primary and bacteria production are found to be in the range of the reported values. Simulation results are in good agreement with in situ data illustrating the role of the physical processes in determining the evolution and variability of the ecosystem. © 2002 Elsevier Science B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The ecosystem function of the oligotrophic Cretan Sea is explored through the development and application of a 3D ecological model. The simulation system comprises of two on-line coupled submodels: the 3D Princeton Ocean Model (POM) and the 1D European Regional Seas Ecosystem Model (ERSEM) adapted to the Cretan Sea. For the tuning and initialisation of the ecosystem parameters, the 1D version of the biogeochemical model is used. After a model spin up period of 10 years to reach a quasi-steady state, the results from an annual simulation are presented. A cost function is used as validation method for the comparison of model results with field data. The estimated annual primary and bacteria production are found to be in the range of the reported values. Simulation results are in good agreement with in situ data illustrating the role of the physical processes in determining the evolution and variability of the ecosystem. © 2002 Elsevier Science B.V. All rights reserved. |
Tsangaris, C; Cotou, E; Papathanassiou, E Multiple biomarker assessment for marine pollution: a case study to distinguish the type of pollutants in Amvrakikos gulf (Greece) Journal Article Marine Environmental Research, 54 , pp. 845, 2002. @article{tsangaris_multiple_2002, title = {Multiple biomarker assessment for marine pollution: a case study to distinguish the type of pollutants in Amvrakikos gulf (Greece)}, author = {C Tsangaris and E Cotou and E Papathanassiou}, year = {2002}, date = {2002-01-01}, journal = {Marine Environmental Research}, volume = {54}, pages = {845}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Tsigenopoulos, C S; Ráb, P; Naran, D; Berrebi, P Multiple origins of polyploidy in the phylogeny of southern African barbs (Cyprinidae) as inferred from mtDNA markers Journal Article Heredity, 88 , pp. 466 – 473, 2002. @article{tsigenopoulos_multiple_2002, title = {Multiple origins of polyploidy in the phylogeny of southern African barbs (Cyprinidae) as inferred from mtDNA markers}, author = {C S Tsigenopoulos and P Ráb and D Naran and P Berrebi}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035988371&partnerID=40&md5=987e5b01eed62d1567a0a38697c6be52}, year = {2002}, date = {2002-01-01}, journal = {Heredity}, volume = {88}, pages = {466 -- 473}, abstract = {The cyprinid genus Barbus, with more than 800 nominal species, is an apparently polyphyletic assemblage to which a number of unrelated species, groups and/or assemblages have been assigned. It includes species that exhibit three different ploidy levels: diploid, tetraploid and hexaploid. Several lineages of the family Cyprinidae constitute a major component of the African freshwater ichthyofauna, having about 500 species, and fishes assigned to the genus ’Barbus’ have the most species on the continent. We used complete sequences of the mitochondrial cytochrome b gene in order to infer phylogenetic relationships between diploid, tetraploid and hexaploid species of ’Barbus’ occurring in southern Africa, the only region where representatives of all of the three ploidy levels occur. The results indicate that most of the lineages are incorrectly classified in the genus ’Barbus’. The southern African tetraploids probably originated from southern African diploids. They constitute a monophyletic group distinct from tetraptoids occurring in the Euro-Mediterranean region (Barbus sensu stricto). The ’small’ African diploid species seem to be paraphyletic, while the ’large’ African hexaploid barbs species are of a single, recent origin and form a monophyletic group. The evidence of multiple, independent origins of polyptoidy occurring in the African cyprinine cyprinids thus provides a significant contribution to the knowledge on the systematic diversity of these fishes, and warrants a thorough taxonomic reorganization of the genus.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The cyprinid genus Barbus, with more than 800 nominal species, is an apparently polyphyletic assemblage to which a number of unrelated species, groups and/or assemblages have been assigned. It includes species that exhibit three different ploidy levels: diploid, tetraploid and hexaploid. Several lineages of the family Cyprinidae constitute a major component of the African freshwater ichthyofauna, having about 500 species, and fishes assigned to the genus ’Barbus’ have the most species on the continent. We used complete sequences of the mitochondrial cytochrome b gene in order to infer phylogenetic relationships between diploid, tetraploid and hexaploid species of ’Barbus’ occurring in southern Africa, the only region where representatives of all of the three ploidy levels occur. The results indicate that most of the lineages are incorrectly classified in the genus ’Barbus’. The southern African tetraploids probably originated from southern African diploids. They constitute a monophyletic group distinct from tetraptoids occurring in the Euro-Mediterranean region (Barbus sensu stricto). The ’small’ African diploid species seem to be paraphyletic, while the ’large’ African hexaploid barbs species are of a single, recent origin and form a monophyletic group. The evidence of multiple, independent origins of polyptoidy occurring in the African cyprinine cyprinids thus provides a significant contribution to the knowledge on the systematic diversity of these fishes, and warrants a thorough taxonomic reorganization of the genus. |
Koutandos, E; Prinos, P Numerical modeling of turbulent free-surface flow over an obstacle Inproceedings pp. 419–427, 2002, (Publication Title: International Conference ‘Riverflow-2002’). @inproceedings{koutandos_numerical_2002, title = {Numerical modeling of turbulent free-surface flow over an obstacle}, author = {E Koutandos and P Prinos}, year = {2002}, date = {2002-01-01}, volume = {1}, pages = {419--427}, note = {Publication Title: International Conference ‘Riverflow-2002’}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Rigos, G; Alexis, M; Andriopoulou, A; Nengas, I Pharmacokinetics and tissue distribution of oxytetracycline in sea bass, dicentrarchus labrax, at two water temperatures Journal Article Aquaculture, 210 , pp. 59 – 67, 2002. @article{rigos_pharmacokinetics_2002, title = {Pharmacokinetics and tissue distribution of oxytetracycline in sea bass, dicentrarchus labrax, at two water temperatures}, author = {G Rigos and M Alexis and A Andriopoulou and I Nengas}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0037205852&partnerID=40&md5=c7df800205f1389fd7180146f3242486}, year = {2002}, date = {2002-01-01}, journal = {Aquaculture}, volume = {210}, pages = {59 -- 67}, abstract = {A pharmacokinetic study of oxytetracycline (OTC) following an intravascular administration (40 mg/kg) was carried out in sea bass, Dicentrarchus labrax (110 g), at 13.5 and 22°C water temperature. Blood, muscle and liver samples were taken at 1, 2, 4, 8, 16, 32, 64 and 128 h post-injection. The plasma data were conformed to a two-compamnent model. The kinetic profile of the drug was found to be temperature dependent. The absorption half-life (t1/2a) of OTC was 0.98 and 0.192 h at 13.5 and 22°C, respectively, whereas the elimination half-time (t1/2β) of the drug was 69 h at 13.5°C and 9.65 h at 22°C. The apparent volume of distribution of the drug at steady state [Vd(ss)] was 5.62 l/kg at 13.5°C and 2.59 l/kg at 22°C. The mean residence time (MRT) of OTC was found to be 37.7 h at 22°C and 71 h at 13.5°C. The total clearance of the drug (CLT) was calculated to be 73.5 and 68.7 ml/kg/h at 13.5 and 22°C, respectively. Liver levels indicated higher OTC values than respective muscle levels at all time points and for both temperatures. The elimination of OTC from tissues tested was faster at the high temperature, whereas the drug was eliminated faster from liver compared to muscle when comparisons are made at the same temperature. © 2002 Elsevier Science B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A pharmacokinetic study of oxytetracycline (OTC) following an intravascular administration (40 mg/kg) was carried out in sea bass, Dicentrarchus labrax (110 g), at 13.5 and 22°C water temperature. Blood, muscle and liver samples were taken at 1, 2, 4, 8, 16, 32, 64 and 128 h post-injection. The plasma data were conformed to a two-compamnent model. The kinetic profile of the drug was found to be temperature dependent. The absorption half-life (t1/2a) of OTC was 0.98 and 0.192 h at 13.5 and 22°C, respectively, whereas the elimination half-time (t1/2β) of the drug was 69 h at 13.5°C and 9.65 h at 22°C. The apparent volume of distribution of the drug at steady state [Vd(ss)] was 5.62 l/kg at 13.5°C and 2.59 l/kg at 22°C. The mean residence time (MRT) of OTC was found to be 37.7 h at 22°C and 71 h at 13.5°C. The total clearance of the drug (CLT) was calculated to be 73.5 and 68.7 ml/kg/h at 13.5 and 22°C, respectively. Liver levels indicated higher OTC values than respective muscle levels at all time points and for both temperatures. The elimination of OTC from tissues tested was faster at the high temperature, whereas the drug was eliminated faster from liver compared to muscle when comparisons are made at the same temperature. © 2002 Elsevier Science B.V. All rights reserved. |
Publications
2003 |
Molecular phylogeny and biogeography of the wall-lizard Podarcis erhardii (Squamata: Lacertidae) Journal Article Molecular Phylogenetics and Evolution, 28 , pp. 38–46, 2003. |
Multiple spawning and egg quality of individual European sea bass (Dicentrarchus labrax) females after repeated injections of GnRHa Journal Article Aquaculture, 221 (1-4), pp. 605–620, 2003, ISSN: 00448486, (Publisher: Elsevier). |
Journal of Chromatography A, 996 (1-2), pp. 163–172, 2003, ISSN: 00219673, (Publisher: Elsevier). |
Pharmacokinetics and bioavailability of oxytetracycline in gilthead sea bream (Sparus aurata) after a single dose Journal Article Aquaculture, 221 , pp. 75 – 83, 2003. |
Pharmacokinetics of flumequine and in vitro activity against bacterial pathogens of gilthead sea bream Sparus aurata Journal Article Diseases of Aquatic Organisms, 54 , pp. 35 – 41, 2003. |
Phylogenetic organization of the genus Barbus sensu stricto: A review based on data using molecular markers Incollection Banarescu, P; Bogutskaya, N G (Ed.): The Freshwater Fishes of Europe, Vol. 5/II: Cyprinidae, Part II: Barbus, pp. 11–22, AULA-Verlag, 2003, (Backup Publisher: AULA-Verlag). |
Biological Journal of the Linnean Society, 80 , pp. 207 – 222, 2003. |
Seasonal changes in sperm production and quality in the red porgy Pagrus pagrus (L.) Journal Article Aquaculture Research, 34 (13), pp. 1161–1170, 2003, ISSN: 1355557X. |
Settlement of the wreckfish (Polyprion americanus) Journal Article Marine Biology, 142 (1), pp. 45–52, 2003, ISSN: 0025-3162. |
Spectral evolution of breaking-non breaking waves in the surf zone Inproceedings pp. 312–313, 2003, (Publication Title: 29th International Conference on Coastal Engineering). |
Structure and dynamics of the macrobenthic crustaceans in the Gialova lagoon (SW Peloponnese) Inproceedings Chersonisos, Crete, Greece, 2003, (Publication Title: Seventh Symposium on Oceanography and Fisheries). |
Testing hypotheses on the biodiversity patterns occurring in the Mediterranean and Black Seas: the example of the benthic polychaetes Inproceedings Chersonisos, Crete, Greece, 2003, (Publication Title: Seventh Symposium on Oceanography and Fisheries). |
The status of Marine Biodiversity in Europe Inproceedings Arvanitidis, C; as representative of, MARBENA Consortium (Ed.): ΙΑSON: International Conference on the Sustainable Development of the Mediterranean and Black Sea Environment, Thessaloniki, Greece, 2003. |
Aquaculture, 224 , pp. 245 – 256, 2003. |
Transport and Atmospheric Processes PCBs in the subtropical atmosphere of Eastern Mediterranean Inproceedings Goldschmidt Conference Abstracts, pp. 18, 447, 2003. |
2002 |
Differentiation of cultured and wild sea bass (Dicentrarchus labrax): total lipid content, fatty acid and trace mineral composition Journal Article Food Chemistry, 79 (2), pp. 145–150, 2002, ISSN: 03088146. |
Kyphosis in reared sea bass (Dicentrarchus labrax L.): ontogeny and effects on mortality Journal Article Aquaculture, 209 (1-4), pp. 49–58, 2002, ISSN: 00448486. |
Journal of Experimental Zoology, 292 (6), pp. 573–579, 2002, ISSN: 0022-104X, 1097-010X. |
Effect of temperature on swimming performance of sea bass juveniles Journal Article Journal of Fish Biology, 60 (4), pp. 923–932, 2002, ISSN: 0022-1112, 1095-8649. |
Molecular Ecology, 11 (4), pp. 755–769, 2002, ISSN: 0962-1083, 1365-294X. |
An automated feeding system for intensive hatcheries Journal Article Aquacultural Engineering, 26 (1), pp. 13–26, 2002, ISSN: 01448609. |
Biological Journal of the Linnean Society, 75 (1), pp. 83–99, 2002, ISSN: 00244066, 10958312. |
Aquaculture, 205 (1-2), pp. 89–102, 2002, ISSN: 00448486. |
A comparison of several biotic indices used for water quality assessment at the Greek rivers Journal Article Fresenius Environmental Bulletin, 11 (11), pp. 1000–1007, 2002, ISSN: 10184619. |
Bulletin of Marine Science, 71 (1), pp. 129–146, 2002, ISSN: 00074977. |
A comperative study of the deep-sea epibenthic fauna from the Balearic (NW Mediterranean) and Ionian (SE Mwediterranean) Basins. Inproceedings Lodz, Poland., 2002, (Publication Title: The 4th European Crustacean Conference.). |
A pharmacokinetic study of flumequine in sea bass, Dicentrarchus labrax (L.), after a single intravascular injection Journal Article Journal of Fish Diseases, 25 , pp. 101 – 105, 2002. |
Environmental Pollution, 119 , pp. 141 – 149, 2002. |
Biological effects of environmental pollution in coastal marine ecosystems: The BEEP Project Journal Article Marine Environmental Research, 54 , pp. 843–844, 2002. |
Biological variation of Loligo vulgaris (Cephalopoda: Loliginidae) in the eastern Atlantic and Mediterranean Journal Article Bulletin of Marine Science, 71 (1), pp. 515–534, 2002, ISSN: 00074977. |
Comparison of freshness quality of cultured and wild sea bass (Dicentrarchus labrax) Journal Article Journal of Food Science, 67 (9), pp. 3220–3226, 2002, ISSN: 0022-1147, 1750-3841. |
Comparison of wild and cultured gilthead sea bream (Sparus aurata); composition, appearance and seasonal variations Journal Article International Journal of Food Science and Technology, 37 (5), pp. 477–484, 2002. |
Contribution des sequences mitochondriales a l’eclatement phylogenetique du genre polyploide Barbus (Teleosteen Cyprinides). Implications biogeographiques Inproceedings Journées Annuelles de la Société Française de Systématique (SFS) ’Systématique et biogéographie’, pp. 49–56, Biosystema, Paris, France, 2002, (Backup Publisher: Biosystema). |
Determination of chloramphenicol residues in gilthead seabream (Sparus aurata L.) tissues by HPLC-PDA Journal Article Journal of Liquid Chromatography and Related Technologies, 25 , pp. 655 – 663, 2002. |
Development of a marine information system for cephalopod fisheries in Eastern Mediterranean Journal Article Bulletin of Marine Science, 71 (2), pp. 867–882, 2002, ISSN: 00074977. |
Enhanced biological performance of intensive sea bream (Sparus aurata) larviculture in the presence of phytoplankton with long photophase Journal Article Aquaculture, 204 , pp. 45 – 63, 2002. |
Floating breakwaters efficiency in intermediate and shallow waters Inproceedings pp. 177–178, 2002, (Publication Title: International Conference ‘Hydroscience-2002’). |
Gas-particle concentrations and distribution of aliphatic hydrocarbons, PAHs, PCBs and PCDD/Fs in the atmosphere of Athens (Greece) Journal Article Atmospheric Environment, 36 (25), pp. 4023–4035, 2002, ISSN: 13522310. |
Genetic structure of a greenhouse population of the spider mite Tetranychus urticae: Spatio-temporal analysis with microsatellite markers Journal Article Insect Molecular Biology, 11 , pp. 157 – 165, 2002. |
Global budget for PCBs provides prediction of their environmental longevities Inproceedings Abstracts of Papers of the American Chemical Society, pp. Part 1–2, ENVR 7, 2002, (Publication Title: Abstracts of Papers of the American Chemical Society Type: Conference Publication Part 1-2, ENVR 7). |
Molecular Ecology, 11 (8), pp. 1499–1514, 2002, ISSN: 09621083. |
Loliginid and ommastrephid stock prediction in greek waters using time series analysis techniques Journal Article Bulletin of Marine Science, 71 (1), pp. 269–287, 2002, ISSN: 00074977. |
Journal of Fish Biology, 60 (2), pp. 328–339, 2002, ISSN: 00221112. |
Modeling robot "psycho-physical" state and reactions - A new option in human-robot communication part 1: Concept and background Journal Article Journal of Intelligent and Robotic Systems: Theory and Applications, 35 , pp. 339 – 352, 2002. |
Modeling robot "psycho-physical" state and reactions - A new option in human-robot communication part 2: Modeling and simulation Journal Article Journal of Intelligent and Robotic Systems: Theory and Applications, 35 , pp. 353 – 364, 2002. |
Modelling the spatial and temporal variability of the Cretan Sea ecosystem Journal Article Journal of Marine Systems, 36 (3-4), pp. 173–196, 2002, ISSN: 09247963. |
Multiple biomarker assessment for marine pollution: a case study to distinguish the type of pollutants in Amvrakikos gulf (Greece) Journal Article Marine Environmental Research, 54 , pp. 845, 2002. |
Multiple origins of polyploidy in the phylogeny of southern African barbs (Cyprinidae) as inferred from mtDNA markers Journal Article Heredity, 88 , pp. 466 – 473, 2002. |
Numerical modeling of turbulent free-surface flow over an obstacle Inproceedings pp. 419–427, 2002, (Publication Title: International Conference ‘Riverflow-2002’). |
Pharmacokinetics and tissue distribution of oxytetracycline in sea bass, dicentrarchus labrax, at two water temperatures Journal Article Aquaculture, 210 , pp. 59 – 67, 2002. |