2002 |
Mañanós, E; Carrillo, M; Sorbera, L A; Mylonas, C C; Asturiano, J F; Bayarri, M J; Zohar, Y; Zanuy, S Journal of Fish Biology, 60 (2), pp. 328–339, 2002, ISSN: 00221112. @article{mananos_luteinizing_2002, title = {Luteinizing hormone and sexual steroid plasma levels after treatment of European sea bass with sustained-release delivery systems for gonadotropin-releasing hormone analogue}, author = {E Mañanós and M Carrillo and L A Sorbera and C C Mylonas and J F Asturiano and M J Bayarri and Y Zohar and S Zanuy}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036464151&doi=10.1006%2fjfbi.2001.1839&partnerID=40&md5=6f51e3fd5612a507457f3a7cfecdc891}, doi = {10.1006/jfbi.2001.1839}, issn = {00221112}, year = {2002}, date = {2002-01-01}, journal = {Journal of Fish Biology}, volume = {60}, number = {2}, pages = {328--339}, abstract = {Spermiating male European sea bass Dicentrarchus labrax were treated with gonadotropin-releasing hormone agonists (GnRHa), either a GnRHa injection (IN; 25 μg kg-1 body mass) or one of three types of controlled-release GnRHa-delivery systems: fast release implants (EVAc; 100 μg kg-1), slow release implants (EVSL; 100 μg kg-1) and slow release microspheres (MC; 50 μg kg-1). Luteinizing hormone (LH) release was highly stimulated by all GnRHa treatments, with elevated plasma levels lasting for 2 days in injected fish (IN) and 2, 4 and 6 weeks in controlled-release-treated fish (EVAc, MC and EVSL, respectively), correlating with a 1, 3, 5 and 5 week period of stimulation of milt production, respectively. Plasma levels of the androgens testosterone (T) and 11-ketotestosterone (11-KT), were not significantly affected by the GnRHa treatments. Plasma T was high at early spermiation and declined sharply near the end of this period. Plasma 11-KT levels declined continuously throughout the experiment. Levels of 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P), a proposed maturation-inducing steroid (MIS) in European sea bass, fluctuated around 0.2-1 ng ml-1 and were not greatly affected by the treatments. These results indicated a close correlation between sustained stimulation of LH release, achieved by GnRHa-delivery systems, and long-term enhancement of milt production. They also show an absence of changes in the common sex steroids, associated with elevated LH and enhanced spermiation. © 2002 The Fisheries Society of the British Isles.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Spermiating male European sea bass Dicentrarchus labrax were treated with gonadotropin-releasing hormone agonists (GnRHa), either a GnRHa injection (IN; 25 μg kg-1 body mass) or one of three types of controlled-release GnRHa-delivery systems: fast release implants (EVAc; 100 μg kg-1), slow release implants (EVSL; 100 μg kg-1) and slow release microspheres (MC; 50 μg kg-1). Luteinizing hormone (LH) release was highly stimulated by all GnRHa treatments, with elevated plasma levels lasting for 2 days in injected fish (IN) and 2, 4 and 6 weeks in controlled-release-treated fish (EVAc, MC and EVSL, respectively), correlating with a 1, 3, 5 and 5 week period of stimulation of milt production, respectively. Plasma levels of the androgens testosterone (T) and 11-ketotestosterone (11-KT), were not significantly affected by the GnRHa treatments. Plasma T was high at early spermiation and declined sharply near the end of this period. Plasma 11-KT levels declined continuously throughout the experiment. Levels of 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P), a proposed maturation-inducing steroid (MIS) in European sea bass, fluctuated around 0.2-1 ng ml-1 and were not greatly affected by the treatments. These results indicated a close correlation between sustained stimulation of LH release, achieved by GnRHa-delivery systems, and long-term enhancement of milt production. They also show an absence of changes in the common sex steroids, associated with elevated LH and enhanced spermiation. © 2002 The Fisheries Society of the British Isles. |
Potkonjak, V; Radojicic, J; Tzafestas, S Modeling robot "psycho-physical" state and reactions - A new option in human-robot communication part 1: Concept and background Journal Article Journal of Intelligent and Robotic Systems: Theory and Applications, 35 , pp. 339 – 352, 2002. @article{potkonjak_modeling_2002-1, title = {Modeling robot "psycho-physical" state and reactions - A new option in human-robot communication part 1: Concept and background}, author = {V Potkonjak and J Radojicic and S Tzafestas}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0036949985&partnerID=40&md5=37ee03a555f18e4b518474fbbb72e240}, year = {2002}, date = {2002-01-01}, journal = {Journal of Intelligent and Robotic Systems: Theory and Applications}, volume = {35}, pages = {339 -- 352}, abstract = {The concept and the theoretical background of the robot psycho-physical states were presented. A robot control scheme was elaborated to feature human-like behaviour to naturally resemble the motion of fatigued humans. The chemical based conditional reactions of human psycho-physical states like fear, stress and fatigue were studied.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The concept and the theoretical background of the robot psycho-physical states were presented. A robot control scheme was elaborated to feature human-like behaviour to naturally resemble the motion of fatigued humans. The chemical based conditional reactions of human psycho-physical states like fear, stress and fatigue were studied. |
Potkonjak, V; Radojicic, J; Tzafestas, S; Kostic, D Modeling robot "psycho-physical" state and reactions - A new option in human-robot communication part 2: Modeling and simulation Journal Article Journal of Intelligent and Robotic Systems: Theory and Applications, 35 , pp. 353 – 364, 2002. @article{potkonjak_modeling_2002, title = {Modeling robot "psycho-physical" state and reactions - A new option in human-robot communication part 2: Modeling and simulation}, author = {V Potkonjak and J Radojicic and S Tzafestas and D Kostic}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0036944969&partnerID=40&md5=e8279bc1eb58ca61ede449cb49241f43}, year = {2002}, date = {2002-01-01}, journal = {Journal of Intelligent and Robotic Systems: Theory and Applications}, volume = {35}, pages = {353 -- 364}, abstract = {This part of the paper examines numerically the possibility of modeling "robot fatigue’’ being representative of a human psychophysical state that can be applied to robots. Temperatures of driving motors are suggested as analogs to fatigue in muscles. Simulation of robot behavior is performed on a typical human task, namely handwriting. Three phases of task execution, characteristic for humans, are observed, i.e. regular motion, reconfiguration after symptoms of fatigue, and degeneration caused by the too long, hard work.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This part of the paper examines numerically the possibility of modeling "robot fatigue’’ being representative of a human psychophysical state that can be applied to robots. Temperatures of driving motors are suggested as analogs to fatigue in muscles. Simulation of robot behavior is performed on a typical human task, namely handwriting. Three phases of task execution, characteristic for humans, are observed, i.e. regular motion, reconfiguration after symptoms of fatigue, and degeneration caused by the too long, hard work. |
Petihakis, G; Triantafyllou, G; Allen, I J; Hoteit, I; Dounas, C Modelling the spatial and temporal variability of the Cretan Sea ecosystem Journal Article Journal of Marine Systems, 36 (3-4), pp. 173–196, 2002, ISSN: 09247963. @article{petihakis_modelling_2002, title = {Modelling the spatial and temporal variability of the Cretan Sea ecosystem}, author = {G Petihakis and G Triantafyllou and I J Allen and I Hoteit and C Dounas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036775648&doi=10.1016%2fS0924-7963%2802%2900186-0&partnerID=40&md5=f1e748d334e8ea4c11407d5a087e6585}, doi = {10.1016/S0924-7963(02)00186-0}, issn = {09247963}, year = {2002}, date = {2002-01-01}, journal = {Journal of Marine Systems}, volume = {36}, number = {3-4}, pages = {173--196}, abstract = {The ecosystem function of the oligotrophic Cretan Sea is explored through the development and application of a 3D ecological model. The simulation system comprises of two on-line coupled submodels: the 3D Princeton Ocean Model (POM) and the 1D European Regional Seas Ecosystem Model (ERSEM) adapted to the Cretan Sea. For the tuning and initialisation of the ecosystem parameters, the 1D version of the biogeochemical model is used. After a model spin up period of 10 years to reach a quasi-steady state, the results from an annual simulation are presented. A cost function is used as validation method for the comparison of model results with field data. The estimated annual primary and bacteria production are found to be in the range of the reported values. Simulation results are in good agreement with in situ data illustrating the role of the physical processes in determining the evolution and variability of the ecosystem. © 2002 Elsevier Science B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The ecosystem function of the oligotrophic Cretan Sea is explored through the development and application of a 3D ecological model. The simulation system comprises of two on-line coupled submodels: the 3D Princeton Ocean Model (POM) and the 1D European Regional Seas Ecosystem Model (ERSEM) adapted to the Cretan Sea. For the tuning and initialisation of the ecosystem parameters, the 1D version of the biogeochemical model is used. After a model spin up period of 10 years to reach a quasi-steady state, the results from an annual simulation are presented. A cost function is used as validation method for the comparison of model results with field data. The estimated annual primary and bacteria production are found to be in the range of the reported values. Simulation results are in good agreement with in situ data illustrating the role of the physical processes in determining the evolution and variability of the ecosystem. © 2002 Elsevier Science B.V. All rights reserved. |
Tsangaris, C; Cotou, E; Papathanassiou, E Multiple biomarker assessment for marine pollution: a case study to distinguish the type of pollutants in Amvrakikos gulf (Greece) Journal Article Marine Environmental Research, 54 , pp. 845, 2002. @article{tsangaris_multiple_2002, title = {Multiple biomarker assessment for marine pollution: a case study to distinguish the type of pollutants in Amvrakikos gulf (Greece)}, author = {C Tsangaris and E Cotou and E Papathanassiou}, year = {2002}, date = {2002-01-01}, journal = {Marine Environmental Research}, volume = {54}, pages = {845}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Tsigenopoulos, C S; Ráb, P; Naran, D; Berrebi, P Multiple origins of polyploidy in the phylogeny of southern African barbs (Cyprinidae) as inferred from mtDNA markers Journal Article Heredity, 88 , pp. 466 – 473, 2002. @article{tsigenopoulos_multiple_2002, title = {Multiple origins of polyploidy in the phylogeny of southern African barbs (Cyprinidae) as inferred from mtDNA markers}, author = {C S Tsigenopoulos and P Ráb and D Naran and P Berrebi}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035988371&partnerID=40&md5=987e5b01eed62d1567a0a38697c6be52}, year = {2002}, date = {2002-01-01}, journal = {Heredity}, volume = {88}, pages = {466 -- 473}, abstract = {The cyprinid genus Barbus, with more than 800 nominal species, is an apparently polyphyletic assemblage to which a number of unrelated species, groups and/or assemblages have been assigned. It includes species that exhibit three different ploidy levels: diploid, tetraploid and hexaploid. Several lineages of the family Cyprinidae constitute a major component of the African freshwater ichthyofauna, having about 500 species, and fishes assigned to the genus ’Barbus’ have the most species on the continent. We used complete sequences of the mitochondrial cytochrome b gene in order to infer phylogenetic relationships between diploid, tetraploid and hexaploid species of ’Barbus’ occurring in southern Africa, the only region where representatives of all of the three ploidy levels occur. The results indicate that most of the lineages are incorrectly classified in the genus ’Barbus’. The southern African tetraploids probably originated from southern African diploids. They constitute a monophyletic group distinct from tetraptoids occurring in the Euro-Mediterranean region (Barbus sensu stricto). The ’small’ African diploid species seem to be paraphyletic, while the ’large’ African hexaploid barbs species are of a single, recent origin and form a monophyletic group. The evidence of multiple, independent origins of polyptoidy occurring in the African cyprinine cyprinids thus provides a significant contribution to the knowledge on the systematic diversity of these fishes, and warrants a thorough taxonomic reorganization of the genus.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The cyprinid genus Barbus, with more than 800 nominal species, is an apparently polyphyletic assemblage to which a number of unrelated species, groups and/or assemblages have been assigned. It includes species that exhibit three different ploidy levels: diploid, tetraploid and hexaploid. Several lineages of the family Cyprinidae constitute a major component of the African freshwater ichthyofauna, having about 500 species, and fishes assigned to the genus ’Barbus’ have the most species on the continent. We used complete sequences of the mitochondrial cytochrome b gene in order to infer phylogenetic relationships between diploid, tetraploid and hexaploid species of ’Barbus’ occurring in southern Africa, the only region where representatives of all of the three ploidy levels occur. The results indicate that most of the lineages are incorrectly classified in the genus ’Barbus’. The southern African tetraploids probably originated from southern African diploids. They constitute a monophyletic group distinct from tetraptoids occurring in the Euro-Mediterranean region (Barbus sensu stricto). The ’small’ African diploid species seem to be paraphyletic, while the ’large’ African hexaploid barbs species are of a single, recent origin and form a monophyletic group. The evidence of multiple, independent origins of polyptoidy occurring in the African cyprinine cyprinids thus provides a significant contribution to the knowledge on the systematic diversity of these fishes, and warrants a thorough taxonomic reorganization of the genus. |
Koutandos, E; Prinos, P Numerical modeling of turbulent free-surface flow over an obstacle Inproceedings pp. 419–427, 2002, (Publication Title: International Conference ‘Riverflow-2002’). @inproceedings{koutandos_numerical_2002, title = {Numerical modeling of turbulent free-surface flow over an obstacle}, author = {E Koutandos and P Prinos}, year = {2002}, date = {2002-01-01}, volume = {1}, pages = {419--427}, note = {Publication Title: International Conference ‘Riverflow-2002’}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Rigos, G; Alexis, M; Andriopoulou, A; Nengas, I Pharmacokinetics and tissue distribution of oxytetracycline in sea bass, dicentrarchus labrax, at two water temperatures Journal Article Aquaculture, 210 , pp. 59 – 67, 2002. @article{rigos_pharmacokinetics_2002, title = {Pharmacokinetics and tissue distribution of oxytetracycline in sea bass, dicentrarchus labrax, at two water temperatures}, author = {G Rigos and M Alexis and A Andriopoulou and I Nengas}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0037205852&partnerID=40&md5=c7df800205f1389fd7180146f3242486}, year = {2002}, date = {2002-01-01}, journal = {Aquaculture}, volume = {210}, pages = {59 -- 67}, abstract = {A pharmacokinetic study of oxytetracycline (OTC) following an intravascular administration (40 mg/kg) was carried out in sea bass, Dicentrarchus labrax (110 g), at 13.5 and 22°C water temperature. Blood, muscle and liver samples were taken at 1, 2, 4, 8, 16, 32, 64 and 128 h post-injection. The plasma data were conformed to a two-compamnent model. The kinetic profile of the drug was found to be temperature dependent. The absorption half-life (t1/2a) of OTC was 0.98 and 0.192 h at 13.5 and 22°C, respectively, whereas the elimination half-time (t1/2β) of the drug was 69 h at 13.5°C and 9.65 h at 22°C. The apparent volume of distribution of the drug at steady state [Vd(ss)] was 5.62 l/kg at 13.5°C and 2.59 l/kg at 22°C. The mean residence time (MRT) of OTC was found to be 37.7 h at 22°C and 71 h at 13.5°C. The total clearance of the drug (CLT) was calculated to be 73.5 and 68.7 ml/kg/h at 13.5 and 22°C, respectively. Liver levels indicated higher OTC values than respective muscle levels at all time points and for both temperatures. The elimination of OTC from tissues tested was faster at the high temperature, whereas the drug was eliminated faster from liver compared to muscle when comparisons are made at the same temperature. © 2002 Elsevier Science B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A pharmacokinetic study of oxytetracycline (OTC) following an intravascular administration (40 mg/kg) was carried out in sea bass, Dicentrarchus labrax (110 g), at 13.5 and 22°C water temperature. Blood, muscle and liver samples were taken at 1, 2, 4, 8, 16, 32, 64 and 128 h post-injection. The plasma data were conformed to a two-compamnent model. The kinetic profile of the drug was found to be temperature dependent. The absorption half-life (t1/2a) of OTC was 0.98 and 0.192 h at 13.5 and 22°C, respectively, whereas the elimination half-time (t1/2β) of the drug was 69 h at 13.5°C and 9.65 h at 22°C. The apparent volume of distribution of the drug at steady state [Vd(ss)] was 5.62 l/kg at 13.5°C and 2.59 l/kg at 22°C. The mean residence time (MRT) of OTC was found to be 37.7 h at 22°C and 71 h at 13.5°C. The total clearance of the drug (CLT) was calculated to be 73.5 and 68.7 ml/kg/h at 13.5 and 22°C, respectively. Liver levels indicated higher OTC values than respective muscle levels at all time points and for both temperatures. The elimination of OTC from tissues tested was faster at the high temperature, whereas the drug was eliminated faster from liver compared to muscle when comparisons are made at the same temperature. © 2002 Elsevier Science B.V. All rights reserved. |
Katharios, P; Iliopoulou-Georgudaki, J; Antimisiaris, S; Kantzaris, V; Pavlidis, M Pharmacokinetics of ivermectin in sea bream, Sparus aurata using a direct competitive ELISA Journal Article Fish Physiology and Biochemistry, 26 (2), pp. 189–195, 2002, ISSN: 09201742. @article{katharios_pharmacokinetics_2002, title = {Pharmacokinetics of ivermectin in sea bream, Sparus aurata using a direct competitive ELISA}, author = {P Katharios and J Iliopoulou-Georgudaki and S Antimisiaris and V Kantzaris and M Pavlidis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0141496418&doi=10.1023%2fA%3a1025473904513&partnerID=40&md5=ceed6774234c000f5932e520562473b6}, doi = {10.1023/A:1025473904513}, issn = {09201742}, year = {2002}, date = {2002-01-01}, journal = {Fish Physiology and Biochemistry}, volume = {26}, number = {2}, pages = {189--195}, abstract = {The pharmacokinetics of ivermectin in the serum of cultured sea bream, Sparus aurata, after a single intraperitoneal injection of 100 μg kg-1 body weight was studied by the use of direct competitive ELISA. Pharmacokinetic analysis of the serum concentrations versus time points obtained was performed using non-comparmental analysis and a compartmental pharmacokinetic model approach. In the latter case a two-compartment open model with a lag time gave the best fitting. The maximum peak serum concentration was 308.4 ng ml-1 at 2 h post treatment. The AUC of ivermectin was 10700 ng h ml-1 and the elimination half-life 15.37 h, indicating a rapid uptake, high bioavailability and fast elimination of the drug by sea bream.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The pharmacokinetics of ivermectin in the serum of cultured sea bream, Sparus aurata, after a single intraperitoneal injection of 100 μg kg-1 body weight was studied by the use of direct competitive ELISA. Pharmacokinetic analysis of the serum concentrations versus time points obtained was performed using non-comparmental analysis and a compartmental pharmacokinetic model approach. In the latter case a two-compartment open model with a lag time gave the best fitting. The maximum peak serum concentration was 308.4 ng ml-1 at 2 h post treatment. The AUC of ivermectin was 10700 ng h ml-1 and the elimination half-life 15.37 h, indicating a rapid uptake, high bioavailability and fast elimination of the drug by sea bream. |
Rigos, G; Alexis, M; Tyrpenou, A E; Nengas, I; Piper, I; Troisi, G Pharmacokinetics of oxolinic acid in gilthead sea bream, Sparus aurata L. Journal Article Journal of Fish Diseases, 25 , pp. 401 – 408, 2002. @article{rigos_pharmacokinetics_2002-1, title = {Pharmacokinetics of oxolinic acid in gilthead sea bream, Sparus aurata L.}, author = {G Rigos and M Alexis and A E Tyrpenou and I Nengas and I Piper and G Troisi}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0036333185&partnerID=40&md5=56977a9d1b32140fe02fdfb38572cf87}, year = {2002}, date = {2002-01-01}, journal = {Journal of Fish Diseases}, volume = {25}, pages = {401 -- 408}, abstract = {This is the first study on the pharmacokinetic parameters of oxolinic acid (OA) in gilthead sea bream, Sparus aurata L. The kinetic profile of OA was studied after a single intravascular injection (20 mg kg-1) in 100 g fish at 20°C. The distribution half-life (t1/2α) and the elimination half-life (t1/2β) of the drug were found to be short (0.51 and 12.60 h, respectively). The drug penetration from the plasma to the tissues was adequate as the apparent volume of distribution of the drug at steady-state (Vd(ss)) was found to be 2.11 L kg-1. The mean residence time (MRT) of OA was short (14.25 h) and the total clearance rate (ClT) of the drug was low (0.15 L kg-1 h-1). The bioavailability (F%) of OA following oral administration (30 mg kg-1) was also low (14%). Maximum values were observed for muscle at 0.5 h after injection, with levels declining as with subsequent sampling. At the first two time points (0.5 and 1 h) plasma levels of OA were higher than muscle, however, the reverse was evident for subsequent samples. Following oral administration, highest musde levels were found at 16 h and, with the exception of the 24-h sampling, musde OA concentrations were higher than plasma at all time points. The fast elimination of OA suggests short withdrawal times with reference to human consumption of treated fish.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This is the first study on the pharmacokinetic parameters of oxolinic acid (OA) in gilthead sea bream, Sparus aurata L. The kinetic profile of OA was studied after a single intravascular injection (20 mg kg-1) in 100 g fish at 20°C. The distribution half-life (t1/2α) and the elimination half-life (t1/2β) of the drug were found to be short (0.51 and 12.60 h, respectively). The drug penetration from the plasma to the tissues was adequate as the apparent volume of distribution of the drug at steady-state (Vd(ss)) was found to be 2.11 L kg-1. The mean residence time (MRT) of OA was short (14.25 h) and the total clearance rate (ClT) of the drug was low (0.15 L kg-1 h-1). The bioavailability (F%) of OA following oral administration (30 mg kg-1) was also low (14%). Maximum values were observed for muscle at 0.5 h after injection, with levels declining as with subsequent sampling. At the first two time points (0.5 and 1 h) plasma levels of OA were higher than muscle, however, the reverse was evident for subsequent samples. Following oral administration, highest musde levels were found at 16 h and, with the exception of the 24-h sampling, musde OA concentrations were higher than plasma at all time points. The fast elimination of OA suggests short withdrawal times with reference to human consumption of treated fish. |
Durand, J -D; Tsigenopoulos, C S; Ünlü, E; Berrebi, P Phylogeny and biogeography of the family cyprinidae in the Middle East inferred from cytochrome b DNA - Evolutionary significance of this region Journal Article Molecular Phylogenetics and Evolution, 22 , pp. 91 – 100, 2002. @article{durand_phylogeny_2002, title = {Phylogeny and biogeography of the family cyprinidae in the Middle East inferred from cytochrome b DNA - Evolutionary significance of this region}, author = {J -D Durand and C S Tsigenopoulos and E Ünlü and P Berrebi}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0036367829&partnerID=40&md5=baef71440c137fcb1a598cc09e6c17c5}, year = {2002}, date = {2002-01-01}, journal = {Molecular Phylogenetics and Evolution}, volume = {22}, pages = {91 -- 100}, abstract = {The phylogenetic relationships of cyprinid species from the Middle East and neighboring biogeographical areas were investigated using cytochrome b sequence variation in order to test hypotheses that consider the Middle Eastern area as an important interchange area or a center of speciation for the freshwater fauna. A total of 62 cyprinid species were analyzed over the complete cytochrome b fragment (1140 bp); 28 belong to the Leuciscinae subfamily and 34 to the Cyprininae. All the Leuciscinae lineage fish recorded in the Middle East are also found in Europe, which was interpreted as an important Palearctic influence in the Middle Eastern ichthyofauna consistent with the Lago Mare dispersion. However, it has also been suggested that several Danube species have their origins in the Middle East. In contrast, the Cyprininae subfamily showed three highly divergent lineages, one shared with the Euro-Mediterranean area (Barbus/Luciobarbus genus) relict of the Lago Mare dispersion, one shared with Africa (Carasobarbus/Varicorhinus subgenus), and the third shared with Asia (Garra genus). Furthermore, clades observed in the phylogenetic reconstructions are not consistent with morphometric or karyological data and disagree with previous taxonomic assumptions. Lastly, the dispersion history in the Middle East of this subfamily appears much more complicated and ancient than that of the Leuciscinae. However, taking into account Cyprininae and Leuciscinae distribution, the Middle East appears more like an important interchange area for the freshwater ichtyofauna than a center of speciation. © 2002 Elsevier Science.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The phylogenetic relationships of cyprinid species from the Middle East and neighboring biogeographical areas were investigated using cytochrome b sequence variation in order to test hypotheses that consider the Middle Eastern area as an important interchange area or a center of speciation for the freshwater fauna. A total of 62 cyprinid species were analyzed over the complete cytochrome b fragment (1140 bp); 28 belong to the Leuciscinae subfamily and 34 to the Cyprininae. All the Leuciscinae lineage fish recorded in the Middle East are also found in Europe, which was interpreted as an important Palearctic influence in the Middle Eastern ichthyofauna consistent with the Lago Mare dispersion. However, it has also been suggested that several Danube species have their origins in the Middle East. In contrast, the Cyprininae subfamily showed three highly divergent lineages, one shared with the Euro-Mediterranean area (Barbus/Luciobarbus genus) relict of the Lago Mare dispersion, one shared with Africa (Carasobarbus/Varicorhinus subgenus), and the third shared with Asia (Garra genus). Furthermore, clades observed in the phylogenetic reconstructions are not consistent with morphometric or karyological data and disagree with previous taxonomic assumptions. Lastly, the dispersion history in the Middle East of this subfamily appears much more complicated and ancient than that of the Leuciscinae. However, taking into account Cyprininae and Leuciscinae distribution, the Middle East appears more like an important interchange area for the freshwater ichtyofauna than a center of speciation. © 2002 Elsevier Science. |
Mandalakis, M; Stephanou, E G Environmental Toxicology and Chemistry, 21 (11), pp. 2270–2275, 2002, ISSN: 07307268. @article{mandalakis_polychlorinated_2002, title = {Polychlorinated biphenyls associated with fine particles (PM2.5) in the urban environment of Chile: Concentration levels, and sampling volatilization losses}, author = {M Mandalakis and E G Stephanou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036837688&partnerID=40&md5=103f99d2532d3e08bec6f200b9fb9303}, issn = {07307268}, year = {2002}, date = {2002-01-01}, journal = {Environmental Toxicology and Chemistry}, volume = {21}, number = {11}, pages = {2270--2275}, abstract = {Polychlorinated biphenyls (PCBs) were measured in fine particles (PM2.5, particles smaller than 2.5 μm in diameter) collected in two urban areas of Chile, Santiago and Temuco, during August and October 1998. A diffusion denuder sampling system was used for the collection of 19 samples. The concentrations of total PCBs (47 congeners) ranged from 672 to 1,790 pg/m3 in the city of Temuco and from 1,159 to 2,750 pg/m3 in Santiago. These concentrations are moderately higher than those observed in other urban areas around the world. High amounts of PCBs may volatilize from fine particles during aerosols sampling using conventional high-volume samplers. Average volatilization losses, determined by the diffusion denuder system, varied between 54 and 97%, showing a strong dependence on partial pressure of individual PCB congeners and air temperature. Overall, our results suggest that measurements of PCBs in the particulate phase of the atmosphere, by using conventional high-volume samplers, might be significantly underestimated because of volatilization losses. The underestimation of PCB and other toxic semivolatile organic compound (SOC) levels associated with fine particles in the urban environment may have consequences to the estimation of health risk posed by inhalation.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Polychlorinated biphenyls (PCBs) were measured in fine particles (PM2.5, particles smaller than 2.5 μm in diameter) collected in two urban areas of Chile, Santiago and Temuco, during August and October 1998. A diffusion denuder sampling system was used for the collection of 19 samples. The concentrations of total PCBs (47 congeners) ranged from 672 to 1,790 pg/m3 in the city of Temuco and from 1,159 to 2,750 pg/m3 in Santiago. These concentrations are moderately higher than those observed in other urban areas around the world. High amounts of PCBs may volatilize from fine particles during aerosols sampling using conventional high-volume samplers. Average volatilization losses, determined by the diffusion denuder system, varied between 54 and 97%, showing a strong dependence on partial pressure of individual PCB congeners and air temperature. Overall, our results suggest that measurements of PCBs in the particulate phase of the atmosphere, by using conventional high-volume samplers, might be significantly underestimated because of volatilization losses. The underestimation of PCB and other toxic semivolatile organic compound (SOC) levels associated with fine particles in the urban environment may have consequences to the estimation of health risk posed by inhalation. |
Koulouri, P Preliminary study of hyperbenthos in Heraklion Bay (Cretan Sea) Journal Article Biomare Newsletter, pp. 12, 2002, (Publisher: Ecological Consultancy Services (Ecoserve) Ltd). @article{koulouri_preliminary_2002, title = {Preliminary study of hyperbenthos in Heraklion Bay (Cretan Sea)}, author = {P Koulouri}, url = {http://www.biomareweb.org/3.6.html}, year = {2002}, date = {2002-01-01}, journal = {Biomare Newsletter}, pages = {12}, note = {Publisher: Ecological Consultancy Services (Ecoserve) Ltd}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Karambas, T; Koutandos, E; Koutitas, C Sea-level rise effects on coastal erosion Inproceedings pp. 387–395, 2002, (Publication Title: International Conference ‘Protection and Restoration of the Environment VI’). @inproceedings{karambas_sea-level_2002, title = {Sea-level rise effects on coastal erosion}, author = {T Karambas and E Koutandos and C Koutitas}, year = {2002}, date = {2002-01-01}, volume = {1}, pages = {387--395}, note = {Publication Title: International Conference ‘Protection and Restoration of the Environment VI’}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Arvanitidis, C; Bellan, G; Drakopoulos, P; Valavanis, V; Dounas, C; Koukouras, A; Eleftheriou, A Seascape biodiversity patterns along the Mediterranean and the Black Sea: Lessons from the biogeography of benthic polychaetes Journal Article Marine Ecology Progress Series, 244 , pp. 139–152, 2002, ISSN: 01718630, (Publisher: Inter-Research). @article{arvanitidis_seascape_2002, title = {Seascape biodiversity patterns along the Mediterranean and the Black Sea: Lessons from the biogeography of benthic polychaetes}, author = {C Arvanitidis and G Bellan and P Drakopoulos and V Valavanis and C Dounas and A Koukouras and A Eleftheriou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037196059&doi=10.3354%2fmeps244139&partnerID=40&md5=24de7c68fd8f96fef3454bcff8471663}, doi = {10.3354/meps244139}, issn = {01718630}, year = {2002}, date = {2002-01-01}, journal = {Marine Ecology Progress Series}, volume = {244}, pages = {139--152}, abstract = {The purpose of this paper is to investigate seascape biodiversity patterns along the Mediterranean and the Black Sea through the study of the benthic polychaete biogeography of the region. A set of non-parametric multivariate analyses and recently developed diversity indices were performed on the benthic polychaete inventories of the areas of the Mediterranean and the Black Sea. Both the numbers of species and the multivariate analyses demonstrate a west-east zoogeocline, shown by the decreasing number of species and by the multivariate similarity pattern of the areas. The performance of the 'second-stage' multi-dimensional scaling (MDS) shows, when species information is aggregated to genera, results belonging practically to the same similarity pattern, independent of the similarity coefficients utilized. The same similarity pattern is derived when species information is aggregated to the zoogeographical categories of amphi-Atlantic, Atlanto-Mediterranean, cosmopolitan and endemic species. BIO-ENV analysis reveals a large number of geographic, climatic and trophic variables to be highly correlated with the similarity pattern derived from the various taxonomic/zoogeographical categories. The synergy, however, of the environmental variables is best reflected in the case of the endemic Mediterranean species. The latter category is considered as the critical zoogeographic category with respect to providing information on the evolutionary history of the taxon in the region. The application of the average taxonomic distinctness and the variation of taxonomic distinctness indices resulted in a diversity ranking of the areas, which appears to be independent of the number of species hosted in each area. However, this is not the case for the phylogenetic diversity (PD) index. Additionally, the former 2 indices indicate that the benthic polychaete species-pool, hosted in the Mediterranean and the Black Sea areas, may well serve as a useful basis for future comparisons in environmental assessment studies. Finally, results from the application of the area-diversity formula show that the equilibrium model can be applicable for the endemic benthic polychaetes of the region: the number of endemic species can be considered as a function of the degree of isolation of the area from the source region against the degree of within-area isolated habitats.}, note = {Publisher: Inter-Research}, keywords = {}, pubstate = {published}, tppubtype = {article} } The purpose of this paper is to investigate seascape biodiversity patterns along the Mediterranean and the Black Sea through the study of the benthic polychaete biogeography of the region. A set of non-parametric multivariate analyses and recently developed diversity indices were performed on the benthic polychaete inventories of the areas of the Mediterranean and the Black Sea. Both the numbers of species and the multivariate analyses demonstrate a west-east zoogeocline, shown by the decreasing number of species and by the multivariate similarity pattern of the areas. The performance of the 'second-stage' multi-dimensional scaling (MDS) shows, when species information is aggregated to genera, results belonging practically to the same similarity pattern, independent of the similarity coefficients utilized. The same similarity pattern is derived when species information is aggregated to the zoogeographical categories of amphi-Atlantic, Atlanto-Mediterranean, cosmopolitan and endemic species. BIO-ENV analysis reveals a large number of geographic, climatic and trophic variables to be highly correlated with the similarity pattern derived from the various taxonomic/zoogeographical categories. The synergy, however, of the environmental variables is best reflected in the case of the endemic Mediterranean species. The latter category is considered as the critical zoogeographic category with respect to providing information on the evolutionary history of the taxon in the region. The application of the average taxonomic distinctness and the variation of taxonomic distinctness indices resulted in a diversity ranking of the areas, which appears to be independent of the number of species hosted in each area. However, this is not the case for the phylogenetic diversity (PD) index. Additionally, the former 2 indices indicate that the benthic polychaete species-pool, hosted in the Mediterranean and the Black Sea areas, may well serve as a useful basis for future comparisons in environmental assessment studies. Finally, results from the application of the area-diversity formula show that the equilibrium model can be applicable for the endemic benthic polychaetes of the region: the number of endemic species can be considered as a function of the degree of isolation of the area from the source region against the degree of within-area isolated habitats. |
Radojičić, J M; Cvetković, D D; Tomović, L M; Džukić, G V; Kalezić, M L Sexual dimorphism in fire-bellied toads Bombina spp. from the central Balkans Journal Article Folia Zoologica, 51 , pp. 129 – 140, 2002. @article{radojicic_sexual_2002, title = {Sexual dimorphism in fire-bellied toads Bombina spp. from the central Balkans}, author = {J M Radojičić and D D Cvetković and L M Tomović and G V Džukić and M L Kalezić}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0036075229&partnerID=40&md5=a67788787bc35ced257a0f62e2156bf1}, year = {2002}, date = {2002-01-01}, journal = {Folia Zoologica}, volume = {51}, pages = {129 -- 140}, abstract = {The direction and level of sexual size dimorphism (SSD) was examined in fire-bellied toads from the central Balkans. Samples were taken from 12 populations: three Bombina bombina, three B. variegata variegata and six B. variegata scabra populations. Intersexual variation of 20 morphometric characters was analysed. In addition, correspondence analysis of eight qualitative characters was performed. The results showed that, though body length had an inconsistent pattern of intersexual variation across taxa, other traits contributed to statistically significant level of SSD. The analysis on multivariate level revealed a highly significant effect of population in all three taxa, and significant effect of sex on character variation. Discriminant analysis confirmed a higher level of intersexual differences in B. variegata compared to B. bombina. Correspondence analysis showed that females and males were similar with respect to qualitative traits in all three taxa. At the univariate level, the most prominent features were: significant differences in tibia length in all three taxa, and highly significant differences in head width in B. bombina and in humerus length in B. v. scabra. These results are discussed with respect to specific reproductive behaviour and possible ecological differences between sexes.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The direction and level of sexual size dimorphism (SSD) was examined in fire-bellied toads from the central Balkans. Samples were taken from 12 populations: three Bombina bombina, three B. variegata variegata and six B. variegata scabra populations. Intersexual variation of 20 morphometric characters was analysed. In addition, correspondence analysis of eight qualitative characters was performed. The results showed that, though body length had an inconsistent pattern of intersexual variation across taxa, other traits contributed to statistically significant level of SSD. The analysis on multivariate level revealed a highly significant effect of population in all three taxa, and significant effect of sex on character variation. Discriminant analysis confirmed a higher level of intersexual differences in B. variegata compared to B. bombina. Correspondence analysis showed that females and males were similar with respect to qualitative traits in all three taxa. At the univariate level, the most prominent features were: significant differences in tibia length in all three taxa, and highly significant differences in head width in B. bombina and in humerus length in B. v. scabra. These results are discussed with respect to specific reproductive behaviour and possible ecological differences between sexes. |
Koutandos, E; Karambas, T; Koutitas, C; Prinos, P Shoreline changes in presence of a floating breakwater Inproceedings pp. 403–410, 2002, (Publication Title: International Conference ‘Protection and Restoration of the Environment VI’). @inproceedings{koutandos_shoreline_2002, title = {Shoreline changes in presence of a floating breakwater}, author = {E Koutandos and T Karambas and C Koutitas and P Prinos}, year = {2002}, date = {2002-01-01}, volume = {1}, pages = {403--410}, note = {Publication Title: International Conference ‘Protection and Restoration of the Environment VI’}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Mandalakis, M; Stephanou, E G Study of atmospheric PCB concentrations over the eastern Mediterranean Sea Journal Article Journal of Geophysical Research: Atmospheres, 107 (D23), 2002, ISSN: 2169897X, (Publisher: Blackwell Publishing Ltd). @article{mandalakis_study_2002, title = {Study of atmospheric PCB concentrations over the eastern Mediterranean Sea}, author = {M Mandalakis and E G Stephanou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-2542452603&doi=10.1029%2f2001JD001566&partnerID=40&md5=c9307efd806bc8c8189a8992004e2a39}, doi = {10.1029/2001JD001566}, issn = {2169897X}, year = {2002}, date = {2002-01-01}, journal = {Journal of Geophysical Research: Atmospheres}, volume = {107}, number = {D23}, abstract = {Air samples were collected between April 1999 and March 2001 at a background marine site in the eastern Mediterranean Sea and analyzed for polychlorinated biphenyls (PCBs). The average concentrations of total PCB congeners (∑PCBs) in the gas and particulate phases of the atmosphere were 68.1 ± 28.8 and 2.3 ± 1.8 pg/m3, respectively. The lack of seasonal variation for the atmospheric concentration of individual congeners and ∑PCBs and the shallow slopes obtained from the Clausius-Clapeyron (CC) plots for several PCB congeners indicated that long-range transport is the main factor controlling the atmospheric levels of PCBs in this area. Most of the episodes with elevated concentrations of ∑PCBs concurred with air transport from western and central Europe. This observation was mostly attributed to the predominance of N-NW winds in eastern Mediterranean. The wind speed apparently did not affect the atmospheric concentration of PCBs. Significant changes observed on the homologue group profile of PCBs were mostly related to the origin of air parcels sampled and during summer were most probably related to the reaction of the lighter congeners with OH radicals. Partitioning of PCBs between gas and particulate phases was well correlated with the subcooled liquid vapor pressure (PLo) for 11 of the 37 samples. The slopes of log Kp versus log PLo ranged between -0.233 and -0.445 and are among the shallowest measured worldwide. This fact may indicate absorption of PCBs in particles covered by a polar film. Copyright 2002 by the American Geophysical Union.}, note = {Publisher: Blackwell Publishing Ltd}, keywords = {}, pubstate = {published}, tppubtype = {article} } Air samples were collected between April 1999 and March 2001 at a background marine site in the eastern Mediterranean Sea and analyzed for polychlorinated biphenyls (PCBs). The average concentrations of total PCB congeners (∑PCBs) in the gas and particulate phases of the atmosphere were 68.1 ± 28.8 and 2.3 ± 1.8 pg/m3, respectively. The lack of seasonal variation for the atmospheric concentration of individual congeners and ∑PCBs and the shallow slopes obtained from the Clausius-Clapeyron (CC) plots for several PCB congeners indicated that long-range transport is the main factor controlling the atmospheric levels of PCBs in this area. Most of the episodes with elevated concentrations of ∑PCBs concurred with air transport from western and central Europe. This observation was mostly attributed to the predominance of N-NW winds in eastern Mediterranean. The wind speed apparently did not affect the atmospheric concentration of PCBs. Significant changes observed on the homologue group profile of PCBs were mostly related to the origin of air parcels sampled and during summer were most probably related to the reaction of the lighter congeners with OH radicals. Partitioning of PCBs between gas and particulate phases was well correlated with the subcooled liquid vapor pressure (PLo) for 11 of the 37 samples. The slopes of log Kp versus log PLo ranged between -0.233 and -0.445 and are among the shallowest measured worldwide. This fact may indicate absorption of PCBs in particles covered by a polar film. Copyright 2002 by the American Geophysical Union. |
Rigos, G; Alexis, M; Andriopoulou, A; Nengas, I Aquaculture Research, 33 , pp. 1175 – 1181, 2002. @article{rigos_temperature-dependent_2002, title = {Temperature-dependent pharmacokinetics and tissue distribution of oxolinic acid in sea bass, Dicentrarchus labrax L., after a single intravascular injection}, author = {G Rigos and M Alexis and A Andriopoulou and I Nengas}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0037180348&partnerID=40&md5=994b617ee638610a5a8b9aef295f8c93}, year = {2002}, date = {2002-01-01}, journal = {Aquaculture Research}, volume = {33}, pages = {1175 -- 1181}, abstract = {The pharmacokinetics and tissue distribution of oxolinic acid following an intravascular administration (15 mg kg-1 fish) were determined in sea bass, Dicentrarchus labrax L. (110g), at 13°C and 22°C water temperature. The kinetic profile of the drug was found to be temperature dependent, with increased temperature having a greater effect on distribution after equilibrium and the elimination phase than on the distribution process. The distibution half-life of oxolinic acid was 1.15 and 2.76h at 22°C and 13°C respectively, whereas the elimination half-life of the drug was 55h at 22°C and 315h at 13°C. The values of the apparent volume of distribution (1.44Lkg-1 at 22°C and 3.31 Lkg-1 at 13°C) and the volume of distribution at steady state (5.2 and 14.7Lkg-1 at the high and low temperature respectively) were considerably different between the two tested temperatures. The total body clearance of the antibiotic was found to be low (1.47Lkg-1 day-1 at 22°C and 0.80Lkg-1 day-1 at 13°C). Lower rates of elimination were found for the liver compared with muscle, the difference increasing with increasing temperature, while elimination rates from the serum were higher than those of other tissues, especially at the high temperature.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The pharmacokinetics and tissue distribution of oxolinic acid following an intravascular administration (15 mg kg-1 fish) were determined in sea bass, Dicentrarchus labrax L. (110g), at 13°C and 22°C water temperature. The kinetic profile of the drug was found to be temperature dependent, with increased temperature having a greater effect on distribution after equilibrium and the elimination phase than on the distribution process. The distibution half-life of oxolinic acid was 1.15 and 2.76h at 22°C and 13°C respectively, whereas the elimination half-life of the drug was 55h at 22°C and 315h at 13°C. The values of the apparent volume of distribution (1.44Lkg-1 at 22°C and 3.31 Lkg-1 at 13°C) and the volume of distribution at steady state (5.2 and 14.7Lkg-1 at the high and low temperature respectively) were considerably different between the two tested temperatures. The total body clearance of the antibiotic was found to be low (1.47Lkg-1 day-1 at 22°C and 0.80Lkg-1 day-1 at 13°C). Lower rates of elimination were found for the liver compared with muscle, the difference increasing with increasing temperature, while elimination rates from the serum were higher than those of other tissues, especially at the high temperature. |
Plaiti, W; Sardá, F; Tselepides, A The contribution of decapod crustaceans to the hyperbenthic fauna of the deep Mediterranean Sea. (poster) Inproceedings Corfu, Greece. 2-6 September., 2002, (Publication Title: The 8th Colloquium Crustacea Decapoda Mediterranea. Type: Poster). @inproceedings{plaiti_contribution_2002, title = {The contribution of decapod crustaceans to the hyperbenthic fauna of the deep Mediterranean Sea. (poster)}, author = {W Plaiti and F Sardá and A Tselepides}, year = {2002}, date = {2002-01-01}, address = {Corfu, Greece. 2-6 September.}, note = {Publication Title: The 8th Colloquium Crustacea Decapoda Mediterranea. Type: Poster}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Venizelos, L; Mouratian, Z; Apostolaki, A; Lagonica, C; Papageorgiou, Nafsika; Manoli, V The Mediterranean, a living sea - A kit for environmental education and awareness for children Inproceedings Miami, Florida, 2002, (Publication Title: 22nd Annual Symposium on Sea Turtle Biology and Conservation Type: Poster). @inproceedings{venizelos_mediterranean_2002, title = {The Mediterranean, a living sea - A kit for environmental education and awareness for children}, author = {L Venizelos and Z Mouratian and A Apostolaki and C Lagonica and Nafsika Papageorgiou and V Manoli}, year = {2002}, date = {2002-01-01}, address = {Miami, Florida}, note = {Publication Title: 22nd Annual Symposium on Sea Turtle Biology and Conservation Type: Poster}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Koutandos, E; Prinos, P Turbulent free-surface flow over a sinusoidal bed Inproceedings pp. 241–246, 2002, (Publication Title: International Conference ‘Hydroinformatics-2002). @inproceedings{koutandos_turbulent_2002, title = {Turbulent free-surface flow over a sinusoidal bed}, author = {E Koutandos and P Prinos}, year = {2002}, date = {2002-01-01}, volume = {1}, pages = {241--246}, note = {Publication Title: International Conference ‘Hydroinformatics-2002}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Kotlik, P; Tsigenopoulos, C S; Rab, P; Berrebi, P Two new Barbus species from the Danube River basin, with redescription of B. petenyi (Teleostei: Cyprinidae) Journal Article Folia Zoologica, 51 (3), pp. 227–240, 2002. @article{kotlik_two_2002, title = {Two new Barbus species from the Danube River basin, with redescription of B. petenyi (Teleostei: Cyprinidae)}, author = {P Kotlik and C S Tsigenopoulos and P Rab and P Berrebi}, year = {2002}, date = {2002-01-01}, journal = {Folia Zoologica}, volume = {51}, number = {3}, pages = {227--240}, abstract = {Three rheophilic species of western Palaearctic textitBarbus are recognised in the Danube River basin, each diagnosed by a set of unique mitochondrial DNA alleles. The three species are parapatrically distributed and morphologically similar to each other. The redescribed textitB. petenyi, from the Eastern and Southern Carpathians and Stara Planina Mts., is distinguished by a short, sharply pointed snout. textitBarbus carpathicus, new species, from the Western and Eastern Carpathians, is distinguished by a long, sharply pointed snout. textitBarbus balcanicus, new species, from the Dinaric Mts. and Western Stara Planina Mts., is distinguished by a short, bluntly pointed snout, and a unique body and fin colour pattern..}, keywords = {}, pubstate = {published}, tppubtype = {article} } Three rheophilic species of western Palaearctic textitBarbus are recognised in the Danube River basin, each diagnosed by a set of unique mitochondrial DNA alleles. The three species are parapatrically distributed and morphologically similar to each other. The redescribed textitB. petenyi, from the Eastern and Southern Carpathians and Stara Planina Mts., is distinguished by a short, sharply pointed snout. textitBarbus carpathicus, new species, from the Western and Eastern Carpathians, is distinguished by a long, sharply pointed snout. textitBarbus balcanicus, new species, from the Dinaric Mts. and Western Stara Planina Mts., is distinguished by a short, bluntly pointed snout, and a unique body and fin colour pattern.. |
Machias, Athanassios; Maraveyia, Eleni; Pavlidis, Michalis; Somarakis, Stylianos; Divanach, Pascal Validation of annuli on scales and otoliths of common dentex (Dentex dentex) Journal Article Fisheries Research, 54 (2), pp. 287–294, 2002, ISSN: 01657836. @article{machias_validation_2002, title = {Validation of annuli on scales and otoliths of common dentex (Dentex dentex)}, author = {Athanassios Machias and Eleni Maraveyia and Michalis Pavlidis and Stylianos Somarakis and Pascal Divanach}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0165783600003027}, doi = {10.1016/S0165-7836(00)00302-7}, issn = {01657836}, year = {2002}, date = {2002-01-01}, urldate = {2020-08-18}, journal = {Fisheries Research}, volume = {54}, number = {2}, pages = {287--294}, abstract = {The formation of annuli was examined from scales and otoliths of farmed (known age) common dentex, Dentex dentex. One scale annulus formed each year during May and one hyaline zone on the otoliths during June. The formation of scale annuli and the hyaline zone on otoliths of adult fish coincided with gonadal maturation which takes place during April to May. Scales were found to be more accurate than otoliths in respect to annuli formation, but their annuli were not as intense and distinct. Missing or additional hyaline zones were observed on adult otoliths. These irregularities were observed after fish maturation and were related to temperature conditions, as well as to faster growth of the farmed fish than the wild ones. © 2002 Elsevier Science B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The formation of annuli was examined from scales and otoliths of farmed (known age) common dentex, Dentex dentex. One scale annulus formed each year during May and one hyaline zone on the otoliths during June. The formation of scale annuli and the hyaline zone on otoliths of adult fish coincided with gonadal maturation which takes place during April to May. Scales were found to be more accurate than otoliths in respect to annuli formation, but their annuli were not as intense and distinct. Missing or additional hyaline zones were observed on adult otoliths. These irregularities were observed after fish maturation and were related to temperature conditions, as well as to faster growth of the farmed fish than the wild ones. © 2002 Elsevier Science B.V. All rights reserved. |
2001 |
Kotzamanis, Y P; Alexis, M N; Andriopoulou, A; Castritsi-Cathariou, I; Fotis, G Utilization of waste material resulting from trout processing in gilthead bream (Sparus aurata L.) diets Journal Article Aquaculture Research, 32 , pp. 288–295, 2001, ISSN: 1355557X, 13652109. @article{kotzamanis_utilization_2001, title = {Utilization of waste material resulting from trout processing in gilthead bream (Sparus aurata L.) diets}, author = {Y P Kotzamanis and M N Alexis and A Andriopoulou and I Castritsi-Cathariou and G Fotis}, url = {http://doi.wiley.com/10.1046/j.1355-557x.2001.00042.x}, doi = {10.1046/j.1355-557x.2001.00042.x}, issn = {1355557X, 13652109}, year = {2001}, date = {2001-12-01}, urldate = {2020-08-18}, journal = {Aquaculture Research}, volume = {32}, pages = {288--295}, abstract = {Fish processing creates a large amount of waste of high nutrient content which, if not properly processed for use in human or animal nutrition, is likely to be deposited in the environment creating pollution problems. Waste parts from rainbow trout processing for smoking, consisting of heads, bones, tails and intestines, were used as feed ingredients for gilthead bream diets. Heads, bones and tails had similar compositions, their weighed mean indicating about 700 g kg-1 moisture, 150 g kg-1 protein and 110 g kg-1 fat. Intestines contained higher lipid (350 g kg-1) and lower moisture (560 g kg-1) and protein content (80 g kg-1). Seasonal changes in composition indicated significant differences. Three experimental diets were formulated having the same proximate composition on a dry weight basis. The control diet (A) contained fish meal as the main protein source and fish oil as the oil supplement. In diet B part of the protein and most of lipid was provided by trout waste and in diet C most of the lipid was provided by trout intestines. Gilthead bream fingerlings of 4 g initial weight were fed to apparent satiation for 72 days, at a temperature of 20°C, to an average final weight of 19 g. All diets were fed in a dry form. The experiment was performed in duplicate. Growth and feed utilization data were high and similar among groups. The body composition of the resulting fish did not show any difference among dietary treatments. Differences in liver lipid and fatty acid content were found between all dietary treatments. The growth and body composition data from this preliminary experiment indicated that trout waste could be used successfully as a dietary ingredient of sea bream diets. © 2001 Blackwell Science Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Fish processing creates a large amount of waste of high nutrient content which, if not properly processed for use in human or animal nutrition, is likely to be deposited in the environment creating pollution problems. Waste parts from rainbow trout processing for smoking, consisting of heads, bones, tails and intestines, were used as feed ingredients for gilthead bream diets. Heads, bones and tails had similar compositions, their weighed mean indicating about 700 g kg-1 moisture, 150 g kg-1 protein and 110 g kg-1 fat. Intestines contained higher lipid (350 g kg-1) and lower moisture (560 g kg-1) and protein content (80 g kg-1). Seasonal changes in composition indicated significant differences. Three experimental diets were formulated having the same proximate composition on a dry weight basis. The control diet (A) contained fish meal as the main protein source and fish oil as the oil supplement. In diet B part of the protein and most of lipid was provided by trout waste and in diet C most of the lipid was provided by trout intestines. Gilthead bream fingerlings of 4 g initial weight were fed to apparent satiation for 72 days, at a temperature of 20°C, to an average final weight of 19 g. All diets were fed in a dry form. The experiment was performed in duplicate. Growth and feed utilization data were high and similar among groups. The body composition of the resulting fish did not show any difference among dietary treatments. Differences in liver lipid and fatty acid content were found between all dietary treatments. The growth and body composition data from this preliminary experiment indicated that trout waste could be used successfully as a dietary ingredient of sea bream diets. © 2001 Blackwell Science Ltd. |
Koumoundouros, G; Sfakianakis, D; Maingot, E; Divanach, P; Kentouri, M Osteological development of the vertebral column and of the fins in Diplodus sargus (Teleostei: Perciformes: Sparidae) Journal Article Marine Biology, 139 (5), pp. 853 – 862, 2001, ISSN: 0025-3162, 1432-1793. @article{koumoundouros_osteological_2001, title = {Osteological development of the vertebral column and of the fins in Diplodus sargus (Teleostei: Perciformes: Sparidae)}, author = {G Koumoundouros and D Sfakianakis and E Maingot and P Divanach and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035654760&partnerID=40&md5=cc6c9efa1262f94c00bbb1f34b0fc21d}, doi = {10.1007/s002270100645}, issn = {0025-3162, 1432-1793}, year = {2001}, date = {2001-11-01}, journal = {Marine Biology}, volume = {139}, number = {5}, pages = {853 -- 862}, abstract = {The osteological development of vertebral column and fins in white sea bream, Diplodus sargus (Linnaeus, 1758) was studied. Vertebral ontogeny started at 5.7 mm total length (TL, post-fixation), with the formation of the first cartilaginous neural and haemal arches, and was completed by the full attainment of dorsal ribs (20.8 mm TL). The formation of vertebral centra occurred between 7.3 and 9.4 mm TL, but was fully completed only after the development of post- and pre-zygapophyses (26.0 mm TL). Pectoral supports were the first fin elements that started to develop (3.8 mm TL), followed by those of the caudal fin (5.2 mm TL), dorsal and anal fins (6.7 mm TL), and then by those of the pelvic fin (9.5 mm TL). The caudal fin of D. sargus was the first to develop fin rays and attain the full count of lepidotrichia (5.6-7.8 mm TL), but the last to attain the full count of dermatotrichia (7.9-16.0 mm TL). The next fins starting to present rays were the pectoral (6.5 mm TL) and the dorsal and anal fins (7.5 mm TL), while the pelvic fins were the last (11.5 mm TL). Following the caudal lepidotrichia (7.8 mm TL), the anal (10.5 mm TL), dorsal (12.0 mm TL), pectoral (11.4 mm TL) and pelvic fins (12.5 mm TL) were the next with fully completed ray counts. Finally, fin meristics were fully developed with the caudal dermatotrichia. The results are discussed in comparison with the fin and vertebral ontogeny of other teleosts.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The osteological development of vertebral column and fins in white sea bream, Diplodus sargus (Linnaeus, 1758) was studied. Vertebral ontogeny started at 5.7 mm total length (TL, post-fixation), with the formation of the first cartilaginous neural and haemal arches, and was completed by the full attainment of dorsal ribs (20.8 mm TL). The formation of vertebral centra occurred between 7.3 and 9.4 mm TL, but was fully completed only after the development of post- and pre-zygapophyses (26.0 mm TL). Pectoral supports were the first fin elements that started to develop (3.8 mm TL), followed by those of the caudal fin (5.2 mm TL), dorsal and anal fins (6.7 mm TL), and then by those of the pelvic fin (9.5 mm TL). The caudal fin of D. sargus was the first to develop fin rays and attain the full count of lepidotrichia (5.6-7.8 mm TL), but the last to attain the full count of dermatotrichia (7.9-16.0 mm TL). The next fins starting to present rays were the pectoral (6.5 mm TL) and the dorsal and anal fins (7.5 mm TL), while the pelvic fins were the last (11.5 mm TL). Following the caudal lepidotrichia (7.8 mm TL), the anal (10.5 mm TL), dorsal (12.0 mm TL), pectoral (11.4 mm TL) and pelvic fins (12.5 mm TL) were the next with fully completed ray counts. Finally, fin meristics were fully developed with the caudal dermatotrichia. The results are discussed in comparison with the fin and vertebral ontogeny of other teleosts. |
Koumoundouros, G; Divanach, P; Anezaki, L; Kentouri, M Temperature-induced ontogenetic plasticity in sea bass (Dicentrarchus labrax) Journal Article Marine Biology, 139 (5), pp. 817 – 830, 2001, ISSN: 0025-3162, 1432-1793. @article{koumoundouros_temperature-induced_2001, title = {Temperature-induced ontogenetic plasticity in sea bass (Dicentrarchus labrax)}, author = {G Koumoundouros and P Divanach and L Anezaki and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035651680&partnerID=40&md5=c0b2c440d241826ebd72b40d6ade80f2}, doi = {10.1007/s002270100635}, issn = {0025-3162, 1432-1793}, year = {2001}, date = {2001-11-01}, journal = {Marine Biology}, volume = {139}, number = {5}, pages = {817 -- 830}, abstract = {We studied the ontogeny of Dicentrarchus labrax comparatively under constant rearing temperatures of 13, 15 and 20°C. At hatching, yolk-sac larval morphometry differed significantly between the temperatures and especially between the two extremes, while at the end of the yolk-sac larval stage, it mainly differed between the two lower temperature regimes and that of 20°C. Compared with the two lower temperature conditions, at 20°C D. labrax presented a significant ontogenetic acceleration which was morphologically expressed either as a significantly smaller total length (TL) at feeding onset, notochord flexion and fin differentiation, or as shifts of the allometric inflection points of 8 out of the 15 morphometric characters studied. Additionally, temperature significantly affected the allometry coefficients, with a decreasing growth intensity as the temperature difference decreased. The rate of TL growth increased under elevated temperature conditions throughout the entire ontogenetic period, except during the early larval period (feeding onset to metamorphosis onset), at which time D. labrax presented equal growth rates at 15°C and 20°C. The results are discussed with respect to the ontogeny of the functional morphology and the meanings of temperature- induced ontogenetic plasticity for the survival of fish larvae.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We studied the ontogeny of Dicentrarchus labrax comparatively under constant rearing temperatures of 13, 15 and 20°C. At hatching, yolk-sac larval morphometry differed significantly between the temperatures and especially between the two extremes, while at the end of the yolk-sac larval stage, it mainly differed between the two lower temperature regimes and that of 20°C. Compared with the two lower temperature conditions, at 20°C D. labrax presented a significant ontogenetic acceleration which was morphologically expressed either as a significantly smaller total length (TL) at feeding onset, notochord flexion and fin differentiation, or as shifts of the allometric inflection points of 8 out of the 15 morphometric characters studied. Additionally, temperature significantly affected the allometry coefficients, with a decreasing growth intensity as the temperature difference decreased. The rate of TL growth increased under elevated temperature conditions throughout the entire ontogenetic period, except during the early larval period (feeding onset to metamorphosis onset), at which time D. labrax presented equal growth rates at 15°C and 20°C. The results are discussed with respect to the ontogeny of the functional morphology and the meanings of temperature- induced ontogenetic plasticity for the survival of fish larvae. |
I., Leonardos; P., Katharios; Charisis, C Age and growth of Carassius auratus gibelio (Linnaeus, 1758) (Pisces: Cyprinidae) in Lake Lysimachia (W. Greece). Inproceedings of Ichthyologists, Panhellenic Society (Ed.): 10th Panhellenic Conference of Ichthyologists, pp. 257–259, Chania, Greece, 2001. @inproceedings{leonardos_age_2001, title = {Age and growth of Carassius auratus gibelio (Linnaeus, 1758) (Pisces: Cyprinidae) in Lake Lysimachia (W. Greece).}, author = {Leonardos I. and Katharios P. and C Charisis}, editor = {Panhellenic Society of Ichthyologists}, year = {2001}, date = {2001-10-01}, booktitle = {10th Panhellenic Conference of Ichthyologists}, pages = {257--259}, address = {Chania, Greece}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Koumoundouros, G; Divanach, P; Kentouri, M The effect of rearing conditions on development of saddleback syndrome and caudal fin deformities in Dentex dentex (L.) Journal Article Aquaculture, 200 (3-4), pp. 285–304, 2001, ISSN: 00448486. @article{koumoundouros_effect_2001, title = {The effect of rearing conditions on development of saddleback syndrome and caudal fin deformities in Dentex dentex (L.)}, author = {G Koumoundouros and P Divanach and M Kentouri}, url = {https://linkinghub.elsevier.com/retrieve/pii/S004484860100552X}, doi = {10.1016/S0044-8486(01)00552-X}, issn = {00448486}, year = {2001}, date = {2001-09-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {200}, number = {3-4}, pages = {285--304}, abstract = {The development of saddleback syndrome and of caudal fin deformities in Dentex dentex (Linnaeus, 1758) was compared under two rearing methods, extensive (E) and semi-extensive (S). The osteological appearance and the meristic characters of the reared fish were compared to that of D. dentex juveniles, collected from the natural environment. All the wild juveniles were normal in respect to their osteological appearance, while the reared specimens presented skeletal deformities resulting from different rearing methods applied. Saddleback syndrome (4.0-4.4%) and severe external deformities of the caudal fin (14.3-15.0%) characterised exclusively the semi-extensive populations, while the extensive populations presented severe abnormalities of the pre-ural centra (25.0-25.6%) with significantly higher frequency than the S reared fish (5.8%). Saddleback syndrome was expressed as a lack of one to all the hard spines of the dorsal fin, accompanied by shape, number and position abnormalities of the related pterygiophores. Caudal fin deformities were mainly characterised by the lack of the upper lepidotrichia or/and dermatotrichia, accompanied by severe deformities of the supporting elements of the upper lobe. The saddleback syndrome and severe caudal fin deformities were anatomically and ontogenetically related to each other, originating at the early larval stage as a result of abnormalities of the primordial marginal finfold and of the posterior tip of the notochord. In respect to the dominant phenotypes, the meristic characters of the normal reared fish were not differentiated from those of the wild, but they presented higher variability. The results are discussed in view of the aetiology, applications and research targets for the erasure of skeletal malformations. © 2001 Elsevier Science B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The development of saddleback syndrome and of caudal fin deformities in Dentex dentex (Linnaeus, 1758) was compared under two rearing methods, extensive (E) and semi-extensive (S). The osteological appearance and the meristic characters of the reared fish were compared to that of D. dentex juveniles, collected from the natural environment. All the wild juveniles were normal in respect to their osteological appearance, while the reared specimens presented skeletal deformities resulting from different rearing methods applied. Saddleback syndrome (4.0-4.4%) and severe external deformities of the caudal fin (14.3-15.0%) characterised exclusively the semi-extensive populations, while the extensive populations presented severe abnormalities of the pre-ural centra (25.0-25.6%) with significantly higher frequency than the S reared fish (5.8%). Saddleback syndrome was expressed as a lack of one to all the hard spines of the dorsal fin, accompanied by shape, number and position abnormalities of the related pterygiophores. Caudal fin deformities were mainly characterised by the lack of the upper lepidotrichia or/and dermatotrichia, accompanied by severe deformities of the supporting elements of the upper lobe. The saddleback syndrome and severe caudal fin deformities were anatomically and ontogenetically related to each other, originating at the early larval stage as a result of abnormalities of the primordial marginal finfold and of the posterior tip of the notochord. In respect to the dominant phenotypes, the meristic characters of the normal reared fish were not differentiated from those of the wild, but they presented higher variability. The results are discussed in view of the aetiology, applications and research targets for the erasure of skeletal malformations. © 2001 Elsevier Science B.V. All rights reserved. |
Palma, J; Alarcon, J A; Alvarez, C; Zouros, E; Magoulas, A; Andrade, J P Developmental stability and genetic heterozygosity in wild and cultured stocks of gilthead sea bream (Sparus aurata) Journal Article Journal of the Marine Biological Association of the United Kingdom, 81 (2), pp. 283–288, 2001, ISSN: 0025-3154, 1469-7769. @article{palma_developmental_2001, title = {Developmental stability and genetic heterozygosity in wild and cultured stocks of gilthead sea bream (Sparus aurata)}, author = {J Palma and J A Alarcon and C Alvarez and E Zouros and A Magoulas and J P Andrade}, url = {https://www.cambridge.org/core/product/identifier/S0025315401003757/type/journal_article}, doi = {10.1017/S0025315401003757}, issn = {0025-3154, 1469-7769}, year = {2001}, date = {2001-04-01}, urldate = {2020-08-09}, journal = {Journal of the Marine Biological Association of the United Kingdom}, volume = {81}, number = {2}, pages = {283--288}, abstract = {The present study checks on the validity of the hypothesis that heterozygosity and the fluctuating asymmetry (FA), common measure of the developmental stability, are linked in populations of wild and cultured stocks of Sparus aurata from five countries. Muscle and liver samples were analysed for variation in 26 allozymes and three microsatellite loci. Pectoral fin rays and upper and lower gill rakers of the first branchial arch were counted on the left and on the right sides of each fish. Fluctuating asymmetry existed in the majority of the samples although their values were consistently low, (0·305±0·147), but higher in the cultured samples. The allozyme heterozygosity values were always high, but lower in the cultured samples. The microsatellite DNA analysis produced similar results. Heterozygosity was higher in cultured individuals (except for the Greek samples). These findings seem to be early evidence that the reared samples are losing some genetic variation, especially due to the loss of the rarest alleles (which were present in the wild populations). Genetic drift, probably caused by propagation practices, is most likely responsible for the decrease of the genetic variation. No distinct pattern of geographic separation was identified.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The present study checks on the validity of the hypothesis that heterozygosity and the fluctuating asymmetry (FA), common measure of the developmental stability, are linked in populations of wild and cultured stocks of Sparus aurata from five countries. Muscle and liver samples were analysed for variation in 26 allozymes and three microsatellite loci. Pectoral fin rays and upper and lower gill rakers of the first branchial arch were counted on the left and on the right sides of each fish. Fluctuating asymmetry existed in the majority of the samples although their values were consistently low, (0·305±0·147), but higher in the cultured samples. The allozyme heterozygosity values were always high, but lower in the cultured samples. The microsatellite DNA analysis produced similar results. Heterozygosity was higher in cultured individuals (except for the Greek samples). These findings seem to be early evidence that the reared samples are losing some genetic variation, especially due to the loss of the rarest alleles (which were present in the wild populations). Genetic drift, probably caused by propagation practices, is most likely responsible for the decrease of the genetic variation. No distinct pattern of geographic separation was identified. |
Navajas, M; Thistlewood, H; Lagnel, J; Marshall, D; Tsagkarakou, A; Pasteur, N Biological Control, 20 (3), pp. 191–198, 2001, ISSN: 10499644. @article{navajas_field_2001, title = {Field releases of the predatory mite Neoseiulus fallacis (Acari : Phytoseiidae) in Canada, monitored by pyrethroid resistance and allozyme markers}, author = {M Navajas and H Thistlewood and J Lagnel and D Marshall and A Tsagkarakou and N Pasteur}, url = {https://linkinghub.elsevier.com/retrieve/pii/S104996440090896X}, doi = {10.1006/bcon.2000.0896}, issn = {10499644}, year = {2001}, date = {2001-03-01}, urldate = {2020-08-18}, journal = {Biological Control}, volume = {20}, number = {3}, pages = {191--198}, abstract = {The predacious phytoseiid mite Neoseiulus fallacis (Garman) is an important agent for the biological control of spider mites in deciduous fruit orchards in North America and Canada. It would be helpful to monitor the fate of released individuals to improve the results of introductions of the predators in biological control trials. We have used two types of genetic markers, pyrethroid resistance and allozymes, for indirect estimation of the survival of N. fallacis introduced in an apple orchard in Ontario, Canada. Mite samples were submitted to toxicological tests. The polymorphism of four enzymes was studied in individual females using an isoelectric focusing technique. A mite sample was taken from the field, mass-reared in the laboratory, and selected for permethrin resistance. This strain was released on several apple trees treated with permethrin, and mite samples were collected from the same trees 10 to 90 days later. The genetic composition and the insecticide resistance level of this sample were compared to those of two other samples from trees where mites had not previously been released, either in the same orchard or in a neighboring block. A control susceptible strain was compared using mites collected earlier from trees on the same site but outside the present experiment. The mites collected from the release trees and those from the strain used for the releases were found to be genetically closely related, as judged from a small genetic distance, and from similar levels of insecticide resistance in both samples. The control samples from the nonrelease trees were genetically distant from these and displayed low resistance levels. These results indicate that the released genotypes established and persisted in the release trees for the period of the experiment. The utility of the two approaches in assessing the fate of released natural enemies is discussed. (C) 2001 Academic Press.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The predacious phytoseiid mite Neoseiulus fallacis (Garman) is an important agent for the biological control of spider mites in deciduous fruit orchards in North America and Canada. It would be helpful to monitor the fate of released individuals to improve the results of introductions of the predators in biological control trials. We have used two types of genetic markers, pyrethroid resistance and allozymes, for indirect estimation of the survival of N. fallacis introduced in an apple orchard in Ontario, Canada. Mite samples were submitted to toxicological tests. The polymorphism of four enzymes was studied in individual females using an isoelectric focusing technique. A mite sample was taken from the field, mass-reared in the laboratory, and selected for permethrin resistance. This strain was released on several apple trees treated with permethrin, and mite samples were collected from the same trees 10 to 90 days later. The genetic composition and the insecticide resistance level of this sample were compared to those of two other samples from trees where mites had not previously been released, either in the same orchard or in a neighboring block. A control susceptible strain was compared using mites collected earlier from trees on the same site but outside the present experiment. The mites collected from the release trees and those from the strain used for the releases were found to be genetically closely related, as judged from a small genetic distance, and from similar levels of insecticide resistance in both samples. The control samples from the nonrelease trees were genetically distant from these and displayed low resistance levels. These results indicate that the released genotypes established and persisted in the release trees for the period of the experiment. The utility of the two approaches in assessing the fate of released natural enemies is discussed. (C) 2001 Academic Press. |
Cotou, E; Castritsi-Catharios, I; Moraitou-Apostolopoulou, M Surfactant-based oil dispersant toxicity to developing nauplii of Artemia: Effects on ATPase enzymatic system Journal Article Chemosphere, 42 (8), pp. 959–964, 2001, ISSN: 00456535. @article{cotou_surfactant-based_2001, title = {Surfactant-based oil dispersant toxicity to developing nauplii of Artemia: Effects on ATPase enzymatic system}, author = {E Cotou and I Castritsi-Catharios and M Moraitou-Apostolopoulou}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0045653500001089}, doi = {10.1016/S0045-6535(00)00108-9}, issn = {00456535}, year = {2001}, date = {2001-03-01}, urldate = {2020-08-18}, journal = {Chemosphere}, volume = {42}, number = {8}, pages = {959--964}, abstract = {The paper deals with the toxicity of a surfactant-based oil dispersant to the ATPase activities of two naupliar stages of Artemia (instar I & II). Both instars were exposed to sub-lethal and lethal concentrations derived from acute toxicity data. The chosen concentrations were near to LOECs and NOECs. An eightfold difference indicated between the instars was instar-exposure time dependent. The most prominent effects were the inhibition and the stimulation of Na+/K+-ATPase and Mg2+-ATPase activities, respectively. The cause of these effects was related to the dispersant components, the surfactants. The pattern stimulation/inhibition of Mg2+-ATPase and Na+/K+-ATPase activities could be used to indicate toxic stress by surfactant-based oil dispersants since previous studies with other contaminants have shown different ATPase activity patterns. © 2001 Elsevier Science Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The paper deals with the toxicity of a surfactant-based oil dispersant to the ATPase activities of two naupliar stages of Artemia (instar I & II). Both instars were exposed to sub-lethal and lethal concentrations derived from acute toxicity data. The chosen concentrations were near to LOECs and NOECs. An eightfold difference indicated between the instars was instar-exposure time dependent. The most prominent effects were the inhibition and the stimulation of Na+/K+-ATPase and Mg2+-ATPase activities, respectively. The cause of these effects was related to the dispersant components, the surfactants. The pattern stimulation/inhibition of Mg2+-ATPase and Na+/K+-ATPase activities could be used to indicate toxic stress by surfactant-based oil dispersants since previous studies with other contaminants have shown different ATPase activity patterns. © 2001 Elsevier Science Ltd. |
Koumoundouros, G; Divanach, P; Kentouri, M Osteological development of Dentex dentex (Osteichthyes: Sparidae): dorsal, anal, paired fins and squamation Journal Article Marine Biology, 138 (2), pp. 399–406, 2001, ISSN: 0025-3162, 1432-1793. @article{koumoundouros_osteological_2001-1, title = {Osteological development of Dentex dentex (Osteichthyes: Sparidae): dorsal, anal, paired fins and squamation}, author = {G Koumoundouros and P Divanach and M Kentouri}, url = {http://link.springer.com/10.1007/s002270000460}, doi = {10.1007/s002270000460}, issn = {0025-3162, 1432-1793}, year = {2001}, date = {2001-02-01}, urldate = {2020-08-18}, journal = {Marine Biology}, volume = {138}, number = {2}, pages = {399--406}, abstract = {The osteological development of dorsal, anal and paired fins and the squamation in common dentex, Dentex dentex (Linnaeus, 1758), was studied under extensive larval rearing conditions. The ontogeny of the fins was determined in three stages: (1) the yolk-sac and the early larval stage (up to 6.7 mm in vivo total length, TL), in which the majority of the elements of the pectoral fins and the predorsals, dorsal and anal proximal pterygiophores formed; (2) the late larval stage (up to 11.6 mm TL), in which the majority of the pectoral lepidotrichia, the lower extrascapulae, all the elements of the pelvic fins, as well as the lepidotrichia, spines and the distal radials of the rest fins developed; and (3) the metamorphosis stage (up to 24.0 mm TL), when the upper extrascapulae formed and the pectoral lepidotrichia attained their full count. The ontogeny of squamation was carried out during the metamorphosis stage; it started at 13.4 mm TL with laterally symmetric points on the middle of each side of the body, followed by one ventral centre (15.6 mm TL) which was located anterior to the pelvic fins, and two bilateral pairs of centres which appeared on the head (17.8 and 21.2 mm TL). The results are discussed from a functional viewpoint and in comparison with the fin and scale ontogeny of other teleosts.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The osteological development of dorsal, anal and paired fins and the squamation in common dentex, Dentex dentex (Linnaeus, 1758), was studied under extensive larval rearing conditions. The ontogeny of the fins was determined in three stages: (1) the yolk-sac and the early larval stage (up to 6.7 mm in vivo total length, TL), in which the majority of the elements of the pectoral fins and the predorsals, dorsal and anal proximal pterygiophores formed; (2) the late larval stage (up to 11.6 mm TL), in which the majority of the pectoral lepidotrichia, the lower extrascapulae, all the elements of the pelvic fins, as well as the lepidotrichia, spines and the distal radials of the rest fins developed; and (3) the metamorphosis stage (up to 24.0 mm TL), when the upper extrascapulae formed and the pectoral lepidotrichia attained their full count. The ontogeny of squamation was carried out during the metamorphosis stage; it started at 13.4 mm TL with laterally symmetric points on the middle of each side of the body, followed by one ventral centre (15.6 mm TL) which was located anterior to the pelvic fins, and two bilateral pairs of centres which appeared on the head (17.8 and 21.2 mm TL). The results are discussed from a functional viewpoint and in comparison with the fin and scale ontogeny of other teleosts. |
Makridis, P; Bergh, Ã; Skjermo, J; Vadstein, O Addition of bacteria bioencapsulated in Artemia metanauplii to a rearing system for halibut larvae Journal Article Aquaculture International, 9 , pp. 225 – 235, 2001. @article{makridis_addition_2001, title = {Addition of bacteria bioencapsulated in Artemia metanauplii to a rearing system for halibut larvae}, author = {P Makridis and à Bergh and J Skjermo and O Vadstein}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035741645&partnerID=40&md5=e90e6486f7d68ec62f82abaa5bb80066}, year = {2001}, date = {2001-01-01}, journal = {Aquaculture International}, volume = {9}, pages = {225 -- 235}, abstract = {The aim of this study was to determine whether it is possible to influence the species composition of the gut microflora in 70-days old halibut larvae (Hippoglossus hippoglossus L.) by addition of bacteria bioencapsulated in Artemia franciscana in two short pulses. Two Vibrio strains, PB 1-11 and PB 6-1 were used. Two treatments received each strain alone, a third treatment received the two bacterial strains in mixture, whereas in a control treatment no bacteria were added. Five fish tanks were included in each treatment. The bacteria were bioencapsulated in Artemia franciscana metanauplii during a short-term incubation in bacterial suspensions of the specific strains, and were added in two pulses, on day 1 and day 10 of the experiment. Addition of bacteria did not increase the total number of colony-forming units (CFU) in the larval gut. The total CFU in the water was lower in tanks added bioencapsulated bacteria than in the control treatment (p textless 0.05). An immunocolony blot assay was used to measure the numbers of the specific bacteria added in samples of water, A. franciscana, and fish larvae. At the end of the experiment on day 13, the strain PB 1-11 reached on average 10% of the total CFU when added alone and 25% when added in mixture with PB 6-1. Strain PB 6-1 reached at the end of the experiment 26.8% of the total CFU when added alone and 24.2% when added in mixture with PB 1-11, whereas the percentages of PB 1-11 and PB 6-1 in the control fish were 12.7% and 10.8% of the total CFU, respectively. However, the differences between the experimental treatments compared with the control group were not significant (p textgreater 0.05). The addition of bioencapsulated bacteria in two pulses within a ten days period was therefore not sufficient to influence the species composition of the microflora of the halibut larvae.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The aim of this study was to determine whether it is possible to influence the species composition of the gut microflora in 70-days old halibut larvae (Hippoglossus hippoglossus L.) by addition of bacteria bioencapsulated in Artemia franciscana in two short pulses. Two Vibrio strains, PB 1-11 and PB 6-1 were used. Two treatments received each strain alone, a third treatment received the two bacterial strains in mixture, whereas in a control treatment no bacteria were added. Five fish tanks were included in each treatment. The bacteria were bioencapsulated in Artemia franciscana metanauplii during a short-term incubation in bacterial suspensions of the specific strains, and were added in two pulses, on day 1 and day 10 of the experiment. Addition of bacteria did not increase the total number of colony-forming units (CFU) in the larval gut. The total CFU in the water was lower in tanks added bioencapsulated bacteria than in the control treatment (p textless 0.05). An immunocolony blot assay was used to measure the numbers of the specific bacteria added in samples of water, A. franciscana, and fish larvae. At the end of the experiment on day 13, the strain PB 1-11 reached on average 10% of the total CFU when added alone and 25% when added in mixture with PB 6-1. Strain PB 6-1 reached at the end of the experiment 26.8% of the total CFU when added alone and 24.2% when added in mixture with PB 1-11, whereas the percentages of PB 1-11 and PB 6-1 in the control fish were 12.7% and 10.8% of the total CFU, respectively. However, the differences between the experimental treatments compared with the control group were not significant (p textgreater 0.05). The addition of bioencapsulated bacteria in two pulses within a ten days period was therefore not sufficient to influence the species composition of the microflora of the halibut larvae. |
Triantafyllou, G; Petihakis, George; Dounas, C; Theodorou, A Assessing marine ecosystem response to nutrient inputs Journal Article Marine Pollution Bulletin, 43 , pp. 175–186, 2001. @article{triantafyllou_assessing_2001, title = {Assessing marine ecosystem response to nutrient inputs}, author = {G Triantafyllou and George Petihakis and C Dounas and A Theodorou}, year = {2001}, date = {2001-01-01}, journal = {Marine Pollution Bulletin}, volume = {43}, pages = {175--186}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Parpoura, A C R; Alexis, M N Effects of different dietary oils in sea bass (Dicentrarchus labrax) nutrition Journal Article Aquaculture International, 9 , pp. 463 – 476, 2001. @article{parpoura_effects_2001, title = {Effects of different dietary oils in sea bass (Dicentrarchus labrax) nutrition}, author = {A C R Parpoura and M N Alexis}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035779783&partnerID=40&md5=f2693ea4761f47fdbe16fdc2575c7510}, doi = {https://doi.org/10.1023/A:1020590701325}, year = {2001}, date = {2001-01-01}, journal = {Aquaculture International}, volume = {9}, pages = {463 -- 476}, abstract = {Five diets having the same proximate composition but containing different types of supplemental oils, singly or in combination, were used for growing sea bass from 95 g to about 200 g in small sea cages. The oils tested were olive oil, soybean oil and fish oil. The diets formulated contained EPA and DHA levels ranging from 0.88 to 1.35% of the diet. Growth parameters and fish body composition were not significantly affected by the type of oil used. The same was generally apparent for liver and hematological characteristics. The content of phospholipids in EPA and DHA was highest in the livers of fish fed diets supplemented with fish oil. A positive correlation was found between dietary and liver n-6 PUFA. Histological sections indicated extended pathological symptoms (intensive liver degeneration and hemorrhages, changes in the gill structure) in the fish receiving diets supplemented only with plant oils. These symptoms existed but to a smaller frequency and degree in livers of fish fed diets supplemented with plant and fish oil, while were not apparent in those fed the fish oil diet.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Five diets having the same proximate composition but containing different types of supplemental oils, singly or in combination, were used for growing sea bass from 95 g to about 200 g in small sea cages. The oils tested were olive oil, soybean oil and fish oil. The diets formulated contained EPA and DHA levels ranging from 0.88 to 1.35% of the diet. Growth parameters and fish body composition were not significantly affected by the type of oil used. The same was generally apparent for liver and hematological characteristics. The content of phospholipids in EPA and DHA was highest in the livers of fish fed diets supplemented with fish oil. A positive correlation was found between dietary and liver n-6 PUFA. Histological sections indicated extended pathological symptoms (intensive liver degeneration and hemorrhages, changes in the gill structure) in the fish receiving diets supplemented only with plant oils. These symptoms existed but to a smaller frequency and degree in livers of fish fed diets supplemented with plant and fish oil, while were not apparent in those fed the fish oil diet. |
Garrigues, P; Narbonne, J F; Budzinski, H; Viarengo, A; Lehtonen, K; Bocquene, G; Anderen, O K; Peters, L; Lowe, D; Cajaraville, M P; Cotou, E; Minier, C; Auffret, M; Rahmani, R Effets biologiques de la pollution environmentale dans les ecosystemes marins cotiers Journal Article Journal de Recherche Océanographique, 26 (3), pp. 31–34, 2001. @article{garrigues_effets_2001, title = {Effets biologiques de la pollution environmentale dans les ecosystemes marins cotiers}, author = {P Garrigues and J F Narbonne and H Budzinski and A Viarengo and K Lehtonen and G Bocquene and O K Anderen and L Peters and D Lowe and M P Cajaraville and E Cotou and C Minier and M Auffret and R Rahmani}, year = {2001}, date = {2001-01-01}, journal = {Journal de Recherche Océanographique}, volume = {26}, number = {3}, pages = {31--34}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Zohar, Y; Mylonas, C C Endocrine manipulations of spawning in cultured fish: From hormones to genes Journal Article Aquaculture, 197 (1-4), pp. 99–136, 2001, ISSN: 00448486. @article{zohar_endocrine_2001, title = {Endocrine manipulations of spawning in cultured fish: From hormones to genes}, author = {Y Zohar and C C Mylonas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035371765&doi=10.1016%2fS0044-8486%2801%2900584-1&partnerID=40&md5=a0f675c73f277d13cecaf3b092505c8c}, doi = {10.1016/S0044-8486(01)00584-1}, issn = {00448486}, year = {2001}, date = {2001-01-01}, journal = {Aquaculture}, volume = {197}, number = {1-4}, pages = {99--136}, abstract = {Almost all fish reared in captivity exhibit some form of reproductive dysfunction. In females, there is often failure to undergo final oocyte maturation, ovulation and spawning; while in males milt production may be reduced and of low quality. These dysfunctions are due to the fact that fish in captivity do not experience the conditions of the spawning grounds, and as a result there is a failure of the pituitary to release the maturational gonadotropin, luteinizing hormone (LH). Reproductive hormones have been utilized since the 1930s to stimulate reproductive processes and induce ovulation/spermiation and spawning. The first methods employed freshly ground pituitaries collected from reproductively mature fish, which contained gonadotropins (mainly LH) and induced steroidogenesis and gonadal maturation. Eventually, purified gonadotropins became available, both of piscine and mammalian origin, e.g., carp or salmon gonadotropin, and human chorionic gonadotropin. In the 1970s, spawning induction methods begun employing the newly discovered gonadotropin-releasing hormone (GnRH), which induces the secretion of the fish's own gonadotropin from the pituitary, thereby overcoming the endocrine failure observed in captive broodstocks. Development of highly potent, synthetic agonists of GnRH (GnRHa) constituted the next generation of hormonal manipulation therapies, and created a surge in the use of hormones to control reproductive processes in aquaculture. The most recent development is the incorporation of GnRHa into polymeric sustained-release delivery systems, which release the hormone over a period of days to weeks. These delivery systems alleviate the need for multiple treatments and induce (a) long-term elevation in sperm production and (b) multiple spawning in fish with asynchronous or multiple-batch group-synchronous ovarian physiology. Based on the recent discovery of GnRH multiplicity in fish and the increasing understanding of its functional significance, new GnRH agonists can be designed for more potent, affordable and physiologically-compatible spawning induction therapies. Future strategies for improved spawning manipulations will be based on understanding the captivity-induced alterations in the GnRH system, and on new approaches for their repair at the level of GnRH gene expression and release. © 2001 Elsevier Science B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Almost all fish reared in captivity exhibit some form of reproductive dysfunction. In females, there is often failure to undergo final oocyte maturation, ovulation and spawning; while in males milt production may be reduced and of low quality. These dysfunctions are due to the fact that fish in captivity do not experience the conditions of the spawning grounds, and as a result there is a failure of the pituitary to release the maturational gonadotropin, luteinizing hormone (LH). Reproductive hormones have been utilized since the 1930s to stimulate reproductive processes and induce ovulation/spermiation and spawning. The first methods employed freshly ground pituitaries collected from reproductively mature fish, which contained gonadotropins (mainly LH) and induced steroidogenesis and gonadal maturation. Eventually, purified gonadotropins became available, both of piscine and mammalian origin, e.g., carp or salmon gonadotropin, and human chorionic gonadotropin. In the 1970s, spawning induction methods begun employing the newly discovered gonadotropin-releasing hormone (GnRH), which induces the secretion of the fish's own gonadotropin from the pituitary, thereby overcoming the endocrine failure observed in captive broodstocks. Development of highly potent, synthetic agonists of GnRH (GnRHa) constituted the next generation of hormonal manipulation therapies, and created a surge in the use of hormones to control reproductive processes in aquaculture. The most recent development is the incorporation of GnRHa into polymeric sustained-release delivery systems, which release the hormone over a period of days to weeks. These delivery systems alleviate the need for multiple treatments and induce (a) long-term elevation in sperm production and (b) multiple spawning in fish with asynchronous or multiple-batch group-synchronous ovarian physiology. Based on the recent discovery of GnRH multiplicity in fish and the increasing understanding of its functional significance, new GnRH agonists can be designed for more potent, affordable and physiologically-compatible spawning induction therapies. Future strategies for improved spawning manipulations will be based on understanding the captivity-induced alterations in the GnRH system, and on new approaches for their repair at the level of GnRH gene expression and release. © 2001 Elsevier Science B.V. |
Mylonas, C C; Zohar, Y Endocrine regulation and artificial induction of oocyte maturation and spermiation in basses of the genus Morone Journal Article Aquaculture, 202 (3-4), pp. 205–220, 2001, ISSN: 00448486. @article{mylonas_endocrine_2001, title = {Endocrine regulation and artificial induction of oocyte maturation and spermiation in basses of the genus Morone}, author = {C C Mylonas and Y Zohar}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035510250&doi=10.1016%2fS0044-8486%2801%2900772-4&partnerID=40&md5=234515ff5965a8f17502acba9688f431}, doi = {10.1016/S0044-8486(01)00772-4}, issn = {00448486}, year = {2001}, date = {2001-01-01}, journal = {Aquaculture}, volume = {202}, number = {3-4}, pages = {205--220}, abstract = {Studying the process of final oocyte maturation (FOM) and spermiation in striped bass (Morone saxatilis), it was determined that the failure to undergo FOM and produce normal amounts of milt in captivity was due to low plasma levels of luteinizing hormone (LH) during the spawning period. As a result, the expected changes in the plasma profiles of the steroids involved in FOM and spermiation do not take place. Based on the hormone profiles and the associated histological examinations, FOM can be separated into an early phase (germinal vesicle (gv) migration) and a late phase (germinal vesicle breakdown). The progestogen 17,20β,21-trihydroxy-4-pregnen-3-one (17,20β,21-P) was identified as the maturation-inducing steroid (MIS), but the high levels of 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P) present during late FOM, suggest a role for this steroid also. In wild striped bass, spermiation was associated with increases in LH and unchanged plasma levels of 17,20β,21-P and 17, 20β-P. In culture, spermiation in both striped bass and white bass (M. chrysops) proceeds under undetectable levels of LH, 17,20β,21-P and 17,20β-P. GnRHa-induced spermiation, however, was associated with increases in plasma LH and 17,20β-P, but not 17,20β,21-P. Treatment of females with GnRHa-delivery systems induced long-term increases in plasma LH and the appropriate changes in sex-steroid hormones. In white bass, GnRHa-delivery systems induced two successive spawns within 3 days from treatment, and in a commercial production facility induced ovulation of 90% of the females, producing 294,500 eggs kg-1 with 81% fertilization. Similarly treated striped bass underwent FOM and ovulation within 10 days after treatment, while tank spawning resulted in the production of 170,000 eggs kg-1 with 47% fertilization success. In male fish, GnRHa-delivery systems induced significant elevations of expressible milt for up to 20 days, resulting in a fourfold increase in production of spermatozoa, without affecting sperm density, motility or fertilization %. © 2001 Published by Elsevier Science B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Studying the process of final oocyte maturation (FOM) and spermiation in striped bass (Morone saxatilis), it was determined that the failure to undergo FOM and produce normal amounts of milt in captivity was due to low plasma levels of luteinizing hormone (LH) during the spawning period. As a result, the expected changes in the plasma profiles of the steroids involved in FOM and spermiation do not take place. Based on the hormone profiles and the associated histological examinations, FOM can be separated into an early phase (germinal vesicle (gv) migration) and a late phase (germinal vesicle breakdown). The progestogen 17,20β,21-trihydroxy-4-pregnen-3-one (17,20β,21-P) was identified as the maturation-inducing steroid (MIS), but the high levels of 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P) present during late FOM, suggest a role for this steroid also. In wild striped bass, spermiation was associated with increases in LH and unchanged plasma levels of 17,20β,21-P and 17, 20β-P. In culture, spermiation in both striped bass and white bass (M. chrysops) proceeds under undetectable levels of LH, 17,20β,21-P and 17,20β-P. GnRHa-induced spermiation, however, was associated with increases in plasma LH and 17,20β-P, but not 17,20β,21-P. Treatment of females with GnRHa-delivery systems induced long-term increases in plasma LH and the appropriate changes in sex-steroid hormones. In white bass, GnRHa-delivery systems induced two successive spawns within 3 days from treatment, and in a commercial production facility induced ovulation of 90% of the females, producing 294,500 eggs kg-1 with 81% fertilization. Similarly treated striped bass underwent FOM and ovulation within 10 days after treatment, while tank spawning resulted in the production of 170,000 eggs kg-1 with 47% fertilization success. In male fish, GnRHa-delivery systems induced significant elevations of expressible milt for up to 20 days, resulting in a fourfold increase in production of spermatozoa, without affecting sperm density, motility or fertilization %. © 2001 Published by Elsevier Science B.V. |
Alasalvar, C; Taylor, K D A; Öksüz, A; Garthwaite, T; Alexis, M N; Grigorakis, K Freshness assessment of cultured sea bream (Sparus aurata) by chemical, physical and sensory methods Journal Article Food Chemistry, 72 (1), pp. 33–40, 2001, ISSN: 03088146. @article{alasalvar_freshness_2001, title = {Freshness assessment of cultured sea bream (Sparus aurata) by chemical, physical and sensory methods}, author = {C Alasalvar and K D A Taylor and A Öksüz and T Garthwaite and M N Alexis and K Grigorakis}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0308814600001965}, doi = {10.1016/S0308-8146(00)00196-5}, issn = {03088146}, year = {2001}, date = {2001-01-01}, urldate = {2020-08-10}, journal = {Food Chemistry}, volume = {72}, number = {1}, pages = {33--40}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Rigos, G; Pavlidis, M; Divanach, P Host susceptibility to Cryptocaryon sp. infection of Mediterranean marine broodfish held under intensive culture conditions: A case report Journal Article Bulletin of the European Association of Fish Pathologists, 21 , pp. 33 – 36, 2001. @article{rigos_host_2001, title = {Host susceptibility to Cryptocaryon sp. infection of Mediterranean marine broodfish held under intensive culture conditions: A case report}, author = {G Rigos and M Pavlidis and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0002831958&partnerID=40&md5=265b03fc4289569f541da14d6b89a6c8}, year = {2001}, date = {2001-01-01}, journal = {Bulletin of the European Association of Fish Pathologists}, volume = {21}, pages = {33 -- 36}, abstract = {The detrimental effect of Cryptocaryon irritans exhibiting a host specificity on several marine fish broodstock is described in this study. The parasite caused considerable mortalities on gilthead seabream, Sparus aurata, common dentex, Dentex dentex, and greater amberjack, Seriola dumerili, while red porgy, Pagrus pagrus, white sea bream, Diplodus sargus, sea bass, Dicentrarchus labrax, and sharpsnout sea bream, Diplodus puntazzo, remained unaffected. Chemical treatments were not found to be effective against the intense parasitosis and only extended, rapid and repeated hyposalinity measures seemed to minimise fish losses.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The detrimental effect of Cryptocaryon irritans exhibiting a host specificity on several marine fish broodstock is described in this study. The parasite caused considerable mortalities on gilthead seabream, Sparus aurata, common dentex, Dentex dentex, and greater amberjack, Seriola dumerili, while red porgy, Pagrus pagrus, white sea bream, Diplodus sargus, sea bass, Dicentrarchus labrax, and sharpsnout sea bream, Diplodus puntazzo, remained unaffected. Chemical treatments were not found to be effective against the intense parasitosis and only extended, rapid and repeated hyposalinity measures seemed to minimise fish losses. |
Panini, E B; Mylonas, C C; Zanuy, S; Carrillo, M; Ramos, J; Bruce, M P Incubation of embryos and larvae of marine fish using microtiter plates Journal Article Aquaculture International, 9 (2), pp. 189–195, 2001, ISSN: 09676120. @article{panini_incubation_2001, title = {Incubation of embryos and larvae of marine fish using microtiter plates}, author = {E B Panini and C C Mylonas and S Zanuy and M Carrillo and J Ramos and M P Bruce}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035574240&doi=10.1023%2fA%3a1014261830098&partnerID=40&md5=f38e791b9ea172d4e363686105d97e50}, doi = {10.1023/A:1014261830098}, issn = {09676120}, year = {2001}, date = {2001-01-01}, journal = {Aquaculture International}, volume = {9}, number = {2}, pages = {189--195}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Salvanes, AGV; Kristoffersen, JB Mesopelagic Fishes Incollection Steele, J; Thorpe, S; Turekian, K (Ed.): Encyclopedia of Ocean Sciences, pp. 1711–1717, Elsevier, 2001, ISBN: 978-0-12-227430-5. @incollection{salvanes_mesopelagic_2001, title = {Mesopelagic Fishes}, author = {AGV Salvanes and JB Kristoffersen}, editor = {J Steele and S Thorpe and K Turekian}, url = {http://www.sciencedirect.com/science/article/pii/B012227430X00012X}, doi = {10.1006/rwos.2001.0012}, isbn = {978-0-12-227430-5}, year = {2001}, date = {2001-01-01}, booktitle = {Encyclopedia of Ocean Sciences}, pages = {1711--1717}, publisher = {Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {incollection} } |
Cotou, E; Vagias, C; Rapti, T; Roussis, V Metallothionein levels in the bivalves Callista chione and Venus verrucosa from two mediterranean sites Journal Article Zeitschrift fur Naturforschung - Section C Journal of Biosciences, 56 , pp. 848 – 852, 2001. @article{cotou_metallothionein_2001, title = {Metallothionein levels in the bivalves Callista chione and Venus verrucosa from two mediterranean sites}, author = {E Cotou and C Vagias and T Rapti and V Roussis}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034761822&partnerID=40&md5=ff39bcb4ee40c3f1b5d1f4520eb3454d}, year = {2001}, date = {2001-01-01}, journal = {Zeitschrift fur Naturforschung - Section C Journal of Biosciences}, volume = {56}, pages = {848 -- 852}, abstract = {Metallothioneins levels (MTs) in the clams Callista chione and Venus verrucosa, collected from two coastal sites in Greece, were determined and quantified by SDS polyacrylamide gel electrophoresis (SDS-PAGE) and a spectrophotometric assay (Ellman’s reaction). SDS-PAGE separation in the digestive gland, which represents the hepato-pancreas in clams, demonstrated the presence of MTs similar to mammalian MT (rabbit liver Cd, Zn-thionein). No other SH-containing proteins apart from the MTs were detected. MT levels quantified by the Ellman’s reaction indicated seasonal variation for both species. The highest values were recorded in the spring and the lowest in the autumn. The seasonal variation and the differences in the MT levels of the two areas seem to be related to the reproductive cycle of the organisms as well as to abiotic factors of each area. Our results show that both C. chione and V. verrucosa have the potential to be used as biomarkers of metal pollution, provided that the influence of the external factors is safely quantified.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Metallothioneins levels (MTs) in the clams Callista chione and Venus verrucosa, collected from two coastal sites in Greece, were determined and quantified by SDS polyacrylamide gel electrophoresis (SDS-PAGE) and a spectrophotometric assay (Ellman’s reaction). SDS-PAGE separation in the digestive gland, which represents the hepato-pancreas in clams, demonstrated the presence of MTs similar to mammalian MT (rabbit liver Cd, Zn-thionein). No other SH-containing proteins apart from the MTs were detected. MT levels quantified by the Ellman’s reaction indicated seasonal variation for both species. The highest values were recorded in the spring and the lowest in the autumn. The seasonal variation and the differences in the MT levels of the two areas seem to be related to the reproductive cycle of the organisms as well as to abiotic factors of each area. Our results show that both C. chione and V. verrucosa have the potential to be used as biomarkers of metal pollution, provided that the influence of the external factors is safely quantified. |
Carrillo, J; Koumoundouros, G; Divanach, P; Martinez, J Morphological malformations of the lateral line in reared gilthead sea bream (Sparus aurata L. 1758) Journal Article Aquaculture, 192 (2-4), pp. 281–290, 2001, ISSN: 00448486. @article{carrillo_morphological_2001, title = {Morphological malformations of the lateral line in reared gilthead sea bream (Sparus aurata L. 1758)}, author = {J Carrillo and G Koumoundouros and P Divanach and J Martinez}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848600004543}, doi = {10.1016/S0044-8486(00)00454-3}, issn = {00448486}, year = {2001}, date = {2001-01-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {192}, number = {2-4}, pages = {281--290}, abstract = {A general description of the morphological modifications of the lateral line in gilthead sea bream, reared under hatchery conditions, is provided. Farm-reared fish were examined and compared to wild fish. We used the presence of a pore on the specialized scales of the lateral line to determine the density and distribution of associated sensory neuromasts. The malformations of the lateral line were: zigzag with missing sector, wavy with missing sector, several scattered missing sectors, and several consecutive missing sectors. These abnormalities occurred in 71.0-86.2% of the reared fish, depending on the hatchery. They did not occur in wild fish. The possible behaviour consequences for gilthead sea bream of deficient sensory reception are considered. In addition, the suitability of these abnormalities for use as individual markers in different types of studies is proposed. © 2001 Elsevier Science B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A general description of the morphological modifications of the lateral line in gilthead sea bream, reared under hatchery conditions, is provided. Farm-reared fish were examined and compared to wild fish. We used the presence of a pore on the specialized scales of the lateral line to determine the density and distribution of associated sensory neuromasts. The malformations of the lateral line were: zigzag with missing sector, wavy with missing sector, several scattered missing sectors, and several consecutive missing sectors. These abnormalities occurred in 71.0-86.2% of the reared fish, depending on the hatchery. They did not occur in wild fish. The possible behaviour consequences for gilthead sea bream of deficient sensory reception are considered. In addition, the suitability of these abnormalities for use as individual markers in different types of studies is proposed. © 2001 Elsevier Science B.V. |
Mandalakis, M; Tsapakis, M; Stephanou, E G Journal of Chromatography A, 925 (1-2), pp. 183–196, 2001, ISSN: 00219673, (Publisher: Elsevier). @article{mandalakis_optimization_2001, title = {Optimization and application of high-resolution gas chromatography with ion trap tandem mass spectrometry to the determination of polychlorinated biphenyls in atmospheric aerosols}, author = {M Mandalakis and M Tsapakis and E G Stephanou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035800692&doi=10.1016%2fS0021-9673%2801%2901033-0&partnerID=40&md5=bc2efef41df1a986a561509985d9f65b}, doi = {10.1016/S0021-9673(01)01033-0}, issn = {00219673}, year = {2001}, date = {2001-01-01}, journal = {Journal of Chromatography A}, volume = {925}, number = {1-2}, pages = {183--196}, abstract = {Optimization of the Finnigan GCQ ion trap mass spectrometry (ITMS) system and a clean-up procedure were carried out in order to apply high-resolution gas chromatography-tandem mass spectrometry for the analysis of polychlorinated biphenyls (PCBs) in aerosols. Six ITMS operating parameters, including isolation time, excitation voltage, excitation time, 'q' value, ion source temperature and electron energy were adjusted in order to optimize the instrument analytical performance. The adjustment of all parameters substantially increased the sensitivity of ITMS in the MS-MS mode. Changes in isolation time did not particularly affect ITMS sensitivity while ion source temperature had the strongest influence. After optimization, a limit of detection of 600 fg/μl with S/N varying from 8 up to 91 was achieved. The application of the optimized ITMS parameters conjointly with the developed clean-up procedure resulted in method detection limits of 10-20 fg/m3 for the determination of PCBs, in the particulate and gas phase of the atmospheric aerosol of background areas in the Eastern Mediterranean and Sweden. Copyright © 2001 Elsevier Science B.V.}, note = {Publisher: Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {article} } Optimization of the Finnigan GCQ ion trap mass spectrometry (ITMS) system and a clean-up procedure were carried out in order to apply high-resolution gas chromatography-tandem mass spectrometry for the analysis of polychlorinated biphenyls (PCBs) in aerosols. Six ITMS operating parameters, including isolation time, excitation voltage, excitation time, 'q' value, ion source temperature and electron energy were adjusted in order to optimize the instrument analytical performance. The adjustment of all parameters substantially increased the sensitivity of ITMS in the MS-MS mode. Changes in isolation time did not particularly affect ITMS sensitivity while ion source temperature had the strongest influence. After optimization, a limit of detection of 600 fg/μl with S/N varying from 8 up to 91 was achieved. The application of the optimized ITMS parameters conjointly with the developed clean-up procedure resulted in method detection limits of 10-20 fg/m3 for the determination of PCBs, in the particulate and gas phase of the atmospheric aerosol of background areas in the Eastern Mediterranean and Sweden. Copyright © 2001 Elsevier Science B.V. |
Greenwood, L N; Scott, A P; Vermeirssen, E L M; Mylonas, C C; Pavlidis, M Plasma steroids in mature common dentex (Dentex dentex) stimulated with a gonadotropin-releasing hormone agonist Journal Article General and Comparative Endocrinology, 123 (1), pp. 1–12, 2001, ISSN: 00166480. @article{greenwood_plasma_2001, title = {Plasma steroids in mature common dentex (Dentex dentex) stimulated with a gonadotropin-releasing hormone agonist}, author = {L N Greenwood and A P Scott and E L M Vermeirssen and C C Mylonas and M Pavlidis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034944955&doi=10.1006%2fgcen.2000.7519&partnerID=40&md5=a67970e64ad41e8b9ec71482ff619e51}, doi = {10.1006/gcen.2000.7519}, issn = {00166480}, year = {2001}, date = {2001-01-01}, journal = {General and Comparative Endocrinology}, volume = {123}, number = {1}, pages = {1--12}, abstract = {The aim of this study was to identify the major C21 steroids produced in vivo during artificially induced final oocyte maturation and spawning in female common dentex (Dentex dentex). During the spawning season, mature females were treated with a gonadotropin-releasing hormone agonist (GnRHa)-loaded delivery system, with or without pimozide (given as a single dose at the beginning of the experiment). Blood samples were collected at various intervals during the experiment and were assayed for GnRHa, 17, 20β-dihydroxy-4-pregnen-3-one (17, 20β-P), and 17, 20β, 21-trihydroxy-4-pregnen-3-one (17, 20β, 21-P). A higher percentage of ovulated females was observed in GnRHa-implanted fish, which produced over 10 times more eggs than controls. Relative fecundity was highest in the GnRHa + pimozide group and lowest in controls. The viability of naturally released eggs was low (2 to 15%) in all groups. Plasma concentrations of 17, 20β-P in GnRHa-implanted fish did not increase, but those in control fish decreased, such that there was a significant difference between control and treated fish between 2 and 10 days after treatment. In another experiment, ovulating common dentex were injected intramuscularly with a single dose of 50 μg kg−1 of GnRHa in saline and were sampled for blood at 0, 3, 6, 12, and 24 h postinjection. A single water sample was taken from the tanks at 9 h postinjection, the tanks having been emptied and refilled at 6 h. Measurements were made of plasma and water concentrations of free and conjugated 17, 20β-P, 17, 20β, 21-P, 17β-oestradiol (E2), and GnRHa (plasma only). The GnRHa injection increased plasma levels of all steroids, with free 17, 20β-P reaching maximal levels within 3 h. GnRHa treatment also increased the amounts of free and conjugated steroids released into the water between 6 and 9 h. © 2001 British Crown.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The aim of this study was to identify the major C21 steroids produced in vivo during artificially induced final oocyte maturation and spawning in female common dentex (Dentex dentex). During the spawning season, mature females were treated with a gonadotropin-releasing hormone agonist (GnRHa)-loaded delivery system, with or without pimozide (given as a single dose at the beginning of the experiment). Blood samples were collected at various intervals during the experiment and were assayed for GnRHa, 17, 20β-dihydroxy-4-pregnen-3-one (17, 20β-P), and 17, 20β, 21-trihydroxy-4-pregnen-3-one (17, 20β, 21-P). A higher percentage of ovulated females was observed in GnRHa-implanted fish, which produced over 10 times more eggs than controls. Relative fecundity was highest in the GnRHa + pimozide group and lowest in controls. The viability of naturally released eggs was low (2 to 15%) in all groups. Plasma concentrations of 17, 20β-P in GnRHa-implanted fish did not increase, but those in control fish decreased, such that there was a significant difference between control and treated fish between 2 and 10 days after treatment. In another experiment, ovulating common dentex were injected intramuscularly with a single dose of 50 μg kg−1 of GnRHa in saline and were sampled for blood at 0, 3, 6, 12, and 24 h postinjection. A single water sample was taken from the tanks at 9 h postinjection, the tanks having been emptied and refilled at 6 h. Measurements were made of plasma and water concentrations of free and conjugated 17, 20β-P, 17, 20β, 21-P, 17β-oestradiol (E2), and GnRHa (plasma only). The GnRHa injection increased plasma levels of all steroids, with free 17, 20β-P reaching maximal levels within 3 h. GnRHa treatment also increased the amounts of free and conjugated steroids released into the water between 6 and 9 h. © 2001 British Crown. |
Kristoffersen, J B; Salvanes, A G V Sexual size dimorphism and sex ratio in Müller's pearlside (Maurolicus muelleri) Journal Article Marine Biology, 138 (6), pp. 1087–1092, 2001, ISSN: 00253162. @article{kristoffersen_sexual_2001, title = {Sexual size dimorphism and sex ratio in Müller's pearlside (Maurolicus muelleri)}, author = {J B Kristoffersen and A G V Salvanes}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034933210&doi=10.1007%2Fs002270000529&partnerID=40&md5=231a7d359b6ca8692eeba623e13d94a0}, doi = {10.1007/s002270000529}, issn = {00253162}, year = {2001}, date = {2001-01-01}, journal = {Marine Biology}, volume = {138}, number = {6}, pages = {1087--1092}, abstract = {From a series of samples from the Norwegian Sea and fjords, we demonstrate sexual size dimorphism in Maurolicus muelleri, and geographical variation in the degree of dimorphism. The dimorphism was smaller in the Norwegian Sea than in the fjord populations. In fjords, males had a lower maximum size and higher mortality than females. One-year-old females weighed on average about 50% more than males in fjords, but this weight difference was reduced to about 10% among 3-year-old fish. Possible causes of this decreasing sexual size dimorphism are discussed.}, keywords = {}, pubstate = {published}, tppubtype = {article} } From a series of samples from the Norwegian Sea and fjords, we demonstrate sexual size dimorphism in Maurolicus muelleri, and geographical variation in the degree of dimorphism. The dimorphism was smaller in the Norwegian Sea than in the fjord populations. In fjords, males had a lower maximum size and higher mortality than females. One-year-old females weighed on average about 50% more than males in fjords, but this weight difference was reduced to about 10% among 3-year-old fish. Possible causes of this decreasing sexual size dimorphism are discussed. |
Forniés, M A; Mañanós, E; Carrillo, M; Rocha, A; Laureau, S; Mylonas, C C; Zohar, Y; Zanuy, S Spawning induction of individual European sea bass females (Dicentrarchus labrax) using different GnRHa-delivery systems Journal Article Aquaculture, 202 (3-4), pp. 221–234, 2001, ISSN: 00448486, (Publisher: Elsevier). @article{fornies_spawning_2001, title = {Spawning induction of individual European sea bass females (Dicentrarchus labrax) using different GnRHa-delivery systems}, author = {M A Forniés and E Mañanós and M Carrillo and A Rocha and S Laureau and C C Mylonas and Y Zohar and S Zanuy}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035509922&doi=10.1016%2fS0044-8486%2801%2900773-6&partnerID=40&md5=9b233b084d0915f643c35513f1180854}, doi = {10.1016/S0044-8486(01)00773-6}, issn = {00448486}, year = {2001}, date = {2001-01-01}, journal = {Aquaculture}, volume = {202}, number = {3-4}, pages = {221--234}, abstract = {The present study evaluated the effect of different delivery systems loaded with gonadotropin-releasing hormone agonist (GnRHa) on spawning induction, and egg and larval quality in female European sea bass (Dicentrarchus labrax). Mature females (mean ± S.E.M. oocyte diameter = 905 ± 10 μm) were treated with [D-Ala6, Pro9 NEt]-mGnRHa using GnRHa-loaded microspheres, Mc (60 μg kg-1 body weight, bw) GnRHa-loaded implants, EVAc (50 μg kg-1 bw) or received a single injection of GnRHa in saline, IN (15 μg kg-1 bw). Controls (C) were injected with saline. Females were allocated individually in 2-m3 tanks with two GnRHa-treated males and were allowed to spawn without further handling. All GnRHa treatments induced spawning 3 days after treatment (d.a.t.), whereas control fish did not spawn during the 21-day experimental period. GnRHa microspheres and implants induced up to four consecutive spawns in one female, whereas GnRHa-injected fish spawned only once. Egg quality seemed relatively lower in all GnRHa-treated groups compared to spontaneously spawning fish (natural spawns, NAT group) maintained in large populations (80 fish in 15-m3 tanks), possibly due to both the isolation of individual females in separate tanks and the stress from handling. Mean egg quality of the first spawns was variable, but similar in all GnRHa treatments (6.6-18.2% buoyancy; 21.7-27.4% hatching). In subsequent spawns, the Mc-treated females produced eggs of higher buoyancy than the EVAc group (20.5 ± 6.0% vs. 2.87 ± 1.14%), and similar to the first spawn of the GnRHa-injected group. Our results indicate that, compared to a single GnRHa injection, GnRHa-loaded microspheres can induce multiple spawns in European sea bass, without a negative effect on egg quality. © 2001 Elsevier Science B.V. All rights reserved.}, note = {Publisher: Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {article} } The present study evaluated the effect of different delivery systems loaded with gonadotropin-releasing hormone agonist (GnRHa) on spawning induction, and egg and larval quality in female European sea bass (Dicentrarchus labrax). Mature females (mean ± S.E.M. oocyte diameter = 905 ± 10 μm) were treated with [D-Ala6, Pro9 NEt]-mGnRHa using GnRHa-loaded microspheres, Mc (60 μg kg-1 body weight, bw) GnRHa-loaded implants, EVAc (50 μg kg-1 bw) or received a single injection of GnRHa in saline, IN (15 μg kg-1 bw). Controls (C) were injected with saline. Females were allocated individually in 2-m3 tanks with two GnRHa-treated males and were allowed to spawn without further handling. All GnRHa treatments induced spawning 3 days after treatment (d.a.t.), whereas control fish did not spawn during the 21-day experimental period. GnRHa microspheres and implants induced up to four consecutive spawns in one female, whereas GnRHa-injected fish spawned only once. Egg quality seemed relatively lower in all GnRHa-treated groups compared to spontaneously spawning fish (natural spawns, NAT group) maintained in large populations (80 fish in 15-m3 tanks), possibly due to both the isolation of individual females in separate tanks and the stress from handling. Mean egg quality of the first spawns was variable, but similar in all GnRHa treatments (6.6-18.2% buoyancy; 21.7-27.4% hatching). In subsequent spawns, the Mc-treated females produced eggs of higher buoyancy than the EVAc group (20.5 ± 6.0% vs. 2.87 ± 1.14%), and similar to the first spawn of the GnRHa-injected group. Our results indicate that, compared to a single GnRHa injection, GnRHa-loaded microspheres can induce multiple spawns in European sea bass, without a negative effect on egg quality. © 2001 Elsevier Science B.V. All rights reserved. |
Wilkes, D; Xie, S Q; Stickland, N C; Alami-Durante, H; Kentouri, M; Sterioti, A; Koumoundouros, G; Fauconneau, B; Goldspink, G Journal of Experimental Biology, 204 , pp. 2763 – 2771, 2001. @article{wilkes_temperature_2001, title = {Temperature and myogenic factor transcript levels during early development determines muscle growth potential in rainbow trout (Oncorhynchus mykiss) and sea bass (Dicentrarchus labrax)}, author = {D Wilkes and S Q Xie and N C Stickland and H Alami-Durante and M Kentouri and A Sterioti and G Koumoundouros and B Fauconneau and G Goldspink}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035745854&partnerID=40&md5=fd20f80568bada767740f83db9f355ac}, year = {2001}, date = {2001-01-01}, journal = {Journal of Experimental Biology}, volume = {204}, pages = {2763 -- 2771}, abstract = {The influence of changes in environmental temperature on the mRNA levels of myogenic regulatory factors (MRFs), i.e. MyoD and myogenin, as well as myosin heavy chain (MyHC) were studied during early larval development in rainbow trout and sea bass. Phosphoimager analysis of northern blots indicated that there is an optimum temperature for the RNA transcript levels of MRF and MyHC RNA in trout and in sea bass larvae. In the trout strain studied, the highest concentration for MRF and MyHC transcripts was found at 8°C rather than 4°C or 20°C. In European sea bass, the highest concentrations of MRF and MyHC mRNA were observed at 15-20°C rather than 13°C. Raising sea bass larvae at 15°C was associated with higher MyHC gene expression as well as a trend towards an increase in total muscle fibre number and higher growth rates after transfer at ambient temperature. Results suggest that mRNA levels of MRF and MyHC can be used to optimise early development. An experiment in which the temperature was changed illustrates the consequence of precise temporal expression of MRF genes in specifying muscle fibre number at critical stages during early development.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The influence of changes in environmental temperature on the mRNA levels of myogenic regulatory factors (MRFs), i.e. MyoD and myogenin, as well as myosin heavy chain (MyHC) were studied during early larval development in rainbow trout and sea bass. Phosphoimager analysis of northern blots indicated that there is an optimum temperature for the RNA transcript levels of MRF and MyHC RNA in trout and in sea bass larvae. In the trout strain studied, the highest concentration for MRF and MyHC transcripts was found at 8°C rather than 4°C or 20°C. In European sea bass, the highest concentrations of MRF and MyHC mRNA were observed at 15-20°C rather than 13°C. Raising sea bass larvae at 15°C was associated with higher MyHC gene expression as well as a trend towards an increase in total muscle fibre number and higher growth rates after transfer at ambient temperature. Results suggest that mRNA levels of MRF and MyHC can be used to optimise early development. An experiment in which the temperature was changed illustrates the consequence of precise temporal expression of MRF genes in specifying muscle fibre number at critical stages during early development. |
Publications
2002 |
Journal of Fish Biology, 60 (2), pp. 328–339, 2002, ISSN: 00221112. |
Modeling robot "psycho-physical" state and reactions - A new option in human-robot communication part 1: Concept and background Journal Article Journal of Intelligent and Robotic Systems: Theory and Applications, 35 , pp. 339 – 352, 2002. |
Modeling robot "psycho-physical" state and reactions - A new option in human-robot communication part 2: Modeling and simulation Journal Article Journal of Intelligent and Robotic Systems: Theory and Applications, 35 , pp. 353 – 364, 2002. |
Modelling the spatial and temporal variability of the Cretan Sea ecosystem Journal Article Journal of Marine Systems, 36 (3-4), pp. 173–196, 2002, ISSN: 09247963. |
Multiple biomarker assessment for marine pollution: a case study to distinguish the type of pollutants in Amvrakikos gulf (Greece) Journal Article Marine Environmental Research, 54 , pp. 845, 2002. |
Multiple origins of polyploidy in the phylogeny of southern African barbs (Cyprinidae) as inferred from mtDNA markers Journal Article Heredity, 88 , pp. 466 – 473, 2002. |
Numerical modeling of turbulent free-surface flow over an obstacle Inproceedings pp. 419–427, 2002, (Publication Title: International Conference ‘Riverflow-2002’). |
Pharmacokinetics and tissue distribution of oxytetracycline in sea bass, dicentrarchus labrax, at two water temperatures Journal Article Aquaculture, 210 , pp. 59 – 67, 2002. |
Pharmacokinetics of ivermectin in sea bream, Sparus aurata using a direct competitive ELISA Journal Article Fish Physiology and Biochemistry, 26 (2), pp. 189–195, 2002, ISSN: 09201742. |
Pharmacokinetics of oxolinic acid in gilthead sea bream, Sparus aurata L. Journal Article Journal of Fish Diseases, 25 , pp. 401 – 408, 2002. |
Phylogeny and biogeography of the family cyprinidae in the Middle East inferred from cytochrome b DNA - Evolutionary significance of this region Journal Article Molecular Phylogenetics and Evolution, 22 , pp. 91 – 100, 2002. |
Environmental Toxicology and Chemistry, 21 (11), pp. 2270–2275, 2002, ISSN: 07307268. |
Preliminary study of hyperbenthos in Heraklion Bay (Cretan Sea) Journal Article Biomare Newsletter, pp. 12, 2002, (Publisher: Ecological Consultancy Services (Ecoserve) Ltd). |
Sea-level rise effects on coastal erosion Inproceedings pp. 387–395, 2002, (Publication Title: International Conference ‘Protection and Restoration of the Environment VI’). |
Seascape biodiversity patterns along the Mediterranean and the Black Sea: Lessons from the biogeography of benthic polychaetes Journal Article Marine Ecology Progress Series, 244 , pp. 139–152, 2002, ISSN: 01718630, (Publisher: Inter-Research). |
Sexual dimorphism in fire-bellied toads Bombina spp. from the central Balkans Journal Article Folia Zoologica, 51 , pp. 129 – 140, 2002. |
Shoreline changes in presence of a floating breakwater Inproceedings pp. 403–410, 2002, (Publication Title: International Conference ‘Protection and Restoration of the Environment VI’). |
Study of atmospheric PCB concentrations over the eastern Mediterranean Sea Journal Article Journal of Geophysical Research: Atmospheres, 107 (D23), 2002, ISSN: 2169897X, (Publisher: Blackwell Publishing Ltd). |
Aquaculture Research, 33 , pp. 1175 – 1181, 2002. |
The contribution of decapod crustaceans to the hyperbenthic fauna of the deep Mediterranean Sea. (poster) Inproceedings Corfu, Greece. 2-6 September., 2002, (Publication Title: The 8th Colloquium Crustacea Decapoda Mediterranea. Type: Poster). |
The Mediterranean, a living sea - A kit for environmental education and awareness for children Inproceedings Miami, Florida, 2002, (Publication Title: 22nd Annual Symposium on Sea Turtle Biology and Conservation Type: Poster). |
Turbulent free-surface flow over a sinusoidal bed Inproceedings pp. 241–246, 2002, (Publication Title: International Conference ‘Hydroinformatics-2002). |
Two new Barbus species from the Danube River basin, with redescription of B. petenyi (Teleostei: Cyprinidae) Journal Article Folia Zoologica, 51 (3), pp. 227–240, 2002. |
Validation of annuli on scales and otoliths of common dentex (Dentex dentex) Journal Article Fisheries Research, 54 (2), pp. 287–294, 2002, ISSN: 01657836. |
2001 |
Utilization of waste material resulting from trout processing in gilthead bream (Sparus aurata L.) diets Journal Article Aquaculture Research, 32 , pp. 288–295, 2001, ISSN: 1355557X, 13652109. |
Osteological development of the vertebral column and of the fins in Diplodus sargus (Teleostei: Perciformes: Sparidae) Journal Article Marine Biology, 139 (5), pp. 853 – 862, 2001, ISSN: 0025-3162, 1432-1793. |
Temperature-induced ontogenetic plasticity in sea bass (Dicentrarchus labrax) Journal Article Marine Biology, 139 (5), pp. 817 – 830, 2001, ISSN: 0025-3162, 1432-1793. |
Age and growth of Carassius auratus gibelio (Linnaeus, 1758) (Pisces: Cyprinidae) in Lake Lysimachia (W. Greece). Inproceedings of Ichthyologists, Panhellenic Society (Ed.): 10th Panhellenic Conference of Ichthyologists, pp. 257–259, Chania, Greece, 2001. |
The effect of rearing conditions on development of saddleback syndrome and caudal fin deformities in Dentex dentex (L.) Journal Article Aquaculture, 200 (3-4), pp. 285–304, 2001, ISSN: 00448486. |
Developmental stability and genetic heterozygosity in wild and cultured stocks of gilthead sea bream (Sparus aurata) Journal Article Journal of the Marine Biological Association of the United Kingdom, 81 (2), pp. 283–288, 2001, ISSN: 0025-3154, 1469-7769. |
Biological Control, 20 (3), pp. 191–198, 2001, ISSN: 10499644. |
Surfactant-based oil dispersant toxicity to developing nauplii of Artemia: Effects on ATPase enzymatic system Journal Article Chemosphere, 42 (8), pp. 959–964, 2001, ISSN: 00456535. |
Osteological development of Dentex dentex (Osteichthyes: Sparidae): dorsal, anal, paired fins and squamation Journal Article Marine Biology, 138 (2), pp. 399–406, 2001, ISSN: 0025-3162, 1432-1793. |
Addition of bacteria bioencapsulated in Artemia metanauplii to a rearing system for halibut larvae Journal Article Aquaculture International, 9 , pp. 225 – 235, 2001. |
Assessing marine ecosystem response to nutrient inputs Journal Article Marine Pollution Bulletin, 43 , pp. 175–186, 2001. |
Effects of different dietary oils in sea bass (Dicentrarchus labrax) nutrition Journal Article Aquaculture International, 9 , pp. 463 – 476, 2001. |
Effets biologiques de la pollution environmentale dans les ecosystemes marins cotiers Journal Article Journal de Recherche Océanographique, 26 (3), pp. 31–34, 2001. |
Endocrine manipulations of spawning in cultured fish: From hormones to genes Journal Article Aquaculture, 197 (1-4), pp. 99–136, 2001, ISSN: 00448486. |
Endocrine regulation and artificial induction of oocyte maturation and spermiation in basses of the genus Morone Journal Article Aquaculture, 202 (3-4), pp. 205–220, 2001, ISSN: 00448486. |
Freshness assessment of cultured sea bream (Sparus aurata) by chemical, physical and sensory methods Journal Article Food Chemistry, 72 (1), pp. 33–40, 2001, ISSN: 03088146. |
Host susceptibility to Cryptocaryon sp. infection of Mediterranean marine broodfish held under intensive culture conditions: A case report Journal Article Bulletin of the European Association of Fish Pathologists, 21 , pp. 33 – 36, 2001. |
Incubation of embryos and larvae of marine fish using microtiter plates Journal Article Aquaculture International, 9 (2), pp. 189–195, 2001, ISSN: 09676120. |
Mesopelagic Fishes Incollection Steele, J; Thorpe, S; Turekian, K (Ed.): Encyclopedia of Ocean Sciences, pp. 1711–1717, Elsevier, 2001, ISBN: 978-0-12-227430-5. |
Metallothionein levels in the bivalves Callista chione and Venus verrucosa from two mediterranean sites Journal Article Zeitschrift fur Naturforschung - Section C Journal of Biosciences, 56 , pp. 848 – 852, 2001. |
Morphological malformations of the lateral line in reared gilthead sea bream (Sparus aurata L. 1758) Journal Article Aquaculture, 192 (2-4), pp. 281–290, 2001, ISSN: 00448486. |
Journal of Chromatography A, 925 (1-2), pp. 183–196, 2001, ISSN: 00219673, (Publisher: Elsevier). |
Plasma steroids in mature common dentex (Dentex dentex) stimulated with a gonadotropin-releasing hormone agonist Journal Article General and Comparative Endocrinology, 123 (1), pp. 1–12, 2001, ISSN: 00166480. |
Sexual size dimorphism and sex ratio in Müller's pearlside (Maurolicus muelleri) Journal Article Marine Biology, 138 (6), pp. 1087–1092, 2001, ISSN: 00253162. |
Spawning induction of individual European sea bass females (Dicentrarchus labrax) using different GnRHa-delivery systems Journal Article Aquaculture, 202 (3-4), pp. 221–234, 2001, ISSN: 00448486, (Publisher: Elsevier). |
Journal of Experimental Biology, 204 , pp. 2763 – 2771, 2001. |