2001 |
Antoniadou, Chryssanthi; Manoudis, G; Dounas, C; Kallianiotis, Argyris; Vidoris, P; Chintiroglou, Chariton Charles The colonization of experimental artificial reefs, from the serpulids polychaetes, in the N. Aegean Sea (Greece). preliminary results Inproceedings Rapp. Comm. Int. Mer Médit., pp. 352, Monaco, 2001. @inproceedings{antoniadou_colonization_2001, title = {The colonization of experimental artificial reefs, from the serpulids polychaetes, in the N. Aegean Sea (Greece). preliminary results}, author = {Chryssanthi Antoniadou and G Manoudis and C Dounas and Argyris Kallianiotis and P Vidoris and Chariton Charles Chintiroglou}, year = {2001}, date = {2001-01-01}, booktitle = {Rapp. Comm. Int. Mer Médit.}, volume = {36}, pages = {352}, address = {Monaco}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Rigos, G; Stevenson, R Aquaculture International, 9 , pp. 247 – 253, 2001. @article{rigos_effect_2001, title = {The effect of antibiotic treatment on the establishment of persistent infection with Yersinia ruckeri Serovar II in rainbow trout Oncorhynchus mykiss (Walbaum)}, author = {G Rigos and R Stevenson}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035741198&partnerID=40&md5=70a523c4134f5aee8b8488ffc10cc738}, year = {2001}, date = {2001-01-01}, journal = {Aquaculture International}, volume = {9}, pages = {247 -- 253}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Pollani, A; Triantafyllou, G; Petihakis, George; Nittis, K; Dounas, C; Koutitas, C The Poseidon operational tool for the prediction of floating pollutant transport Journal Article Marine Pollution Bulletin, 43 , pp. 270–278, 2001. @article{pollani_poseidon_2001, title = {The Poseidon operational tool for the prediction of floating pollutant transport}, author = {A Pollani and G Triantafyllou and George Petihakis and K Nittis and C Dounas and C Koutitas}, year = {2001}, date = {2001-01-01}, journal = {Marine Pollution Bulletin}, volume = {43}, pages = {270--278}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Papandroulakis, N; Divanach, P; Anastasiadis, P; Kentouri, M The pseudo-green water technique for intensive rearing of sea bream (Sparus aurata) larvae Journal Article Aquaculture International, 9 , pp. 205 – 216, 2001. @article{papandroulakis_pseudo-green_2001, title = {The pseudo-green water technique for intensive rearing of sea bream (Sparus aurata) larvae}, author = {N Papandroulakis and P Divanach and P Anastasiadis and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035741183&partnerID=40&md5=25026d07e6e77837a60e0648627158db}, year = {2001}, date = {2001-01-01}, journal = {Aquaculture International}, volume = {9}, pages = {205 -- 216}, abstract = {The "pseudo green water" technology for sea bream (Sparus aurata) larval rearing was evaluated by analyzing results of a 2-year study, performed in a pilot scale system. The technology is characterized by the daily addition of phytoplankton in the rearing tanks during the first month of rearing. Effects of egg origin, spawning season and initial larval density on the larval rearing were investigated. Fish reared in "pseudo green water" systems for 60 days, presented high biological performance in terms of survival (56 ± 16%), mean weight (62 ± 12 mg), total length (20 ± 1 mm), conformity with wild standard (88 ± 9%), and functional swim bladder (93 ± 4%). The results present homogenity between the categories (origin of eggs, time of spawning, initial egg density) studied, proving the stability of the technology.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The "pseudo green water" technology for sea bream (Sparus aurata) larval rearing was evaluated by analyzing results of a 2-year study, performed in a pilot scale system. The technology is characterized by the daily addition of phytoplankton in the rearing tanks during the first month of rearing. Effects of egg origin, spawning season and initial larval density on the larval rearing were investigated. Fish reared in "pseudo green water" systems for 60 days, presented high biological performance in terms of survival (56 ± 16%), mean weight (62 ± 12 mg), total length (20 ± 1 mm), conformity with wild standard (88 ± 9%), and functional swim bladder (93 ± 4%). The results present homogenity between the categories (origin of eggs, time of spawning, initial egg density) studied, proving the stability of the technology. |
Katharios, P; Iliopoulou-Georgudaki, J; Kapata-Zoumbos, K; Spiropoulos, S Toxicity of intraperitoneally injected ivermectin in sea bream, Sparus aurata Journal Article Fish Physiology and Biochemistry, 25 (2), pp. 99–108, 2001, ISSN: 09201742. @article{katharios_toxicity_2001, title = {Toxicity of intraperitoneally injected ivermectin in sea bream, Sparus aurata}, author = {P Katharios and J Iliopoulou-Georgudaki and K Kapata-Zoumbos and S Spiropoulos}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-14244271014&doi=10.1023%2fA%3a1020574810332&partnerID=40&md5=07b57b30739180b79477443f43f5a1e5}, doi = {10.1023/A:1020574810332}, issn = {09201742}, year = {2001}, date = {2001-01-01}, journal = {Fish Physiology and Biochemistry}, volume = {25}, number = {2}, pages = {99--108}, abstract = {Ivermectin has been widely used as a treatment against sea lice infections of farmed fish (salmonids and non-salmonids) without any official license for use in aquaculture. This study presents data on the toxic side effects of ivermectin administered intraperitoneally in sea bream, Sparus aurata at single doses of 100, 200, 400 and 800 μg ivermectin kg-1 fish. The toxicity of ivermectin was evaluated for a period of 35 days post treatment by determining changes in selected hematological parameters (hematocrit, hemoglobin, red blood cell count, leucocyte count, differential white blood cell count, plasma glucose, electrolytes, urea and alkaline phosphatase), pathological effects in the tissues and the organs of the fish using histological techniques and changes in the behavior of the fish. The results showed a decrease of the hematocrit value one hour after the injection for most of the doses along with an increase of the hemoglobin concentration, the number of the white blood cells, the lymphocytes and monocytes and plasma glucose. No mortality or any histopathological alteration at the tissues examined, were recorded. The fish, which were injected with the highest dose of ivermectin, experienced a loss of appetite, lethargy and darkening of their color during the first week post injection. All the studied parameters reached the normal level by the end of the experiment indicating a low and reversible toxicity of the drug.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Ivermectin has been widely used as a treatment against sea lice infections of farmed fish (salmonids and non-salmonids) without any official license for use in aquaculture. This study presents data on the toxic side effects of ivermectin administered intraperitoneally in sea bream, Sparus aurata at single doses of 100, 200, 400 and 800 μg ivermectin kg-1 fish. The toxicity of ivermectin was evaluated for a period of 35 days post treatment by determining changes in selected hematological parameters (hematocrit, hemoglobin, red blood cell count, leucocyte count, differential white blood cell count, plasma glucose, electrolytes, urea and alkaline phosphatase), pathological effects in the tissues and the organs of the fish using histological techniques and changes in the behavior of the fish. The results showed a decrease of the hematocrit value one hour after the injection for most of the doses along with an increase of the hemoglobin concentration, the number of the white blood cells, the lymphocytes and monocytes and plasma glucose. No mortality or any histopathological alteration at the tissues examined, were recorded. The fish, which were injected with the highest dose of ivermectin, experienced a loss of appetite, lethargy and darkening of their color during the first week post injection. All the studied parameters reached the normal level by the end of the experiment indicating a low and reversible toxicity of the drug. |
Bergh, Ø; Vikanes, L; Makridis, P; Skjermo, J; Knappskog, D; Rødseth, O M Uptake and processing of a Vibrio anguillarum bacterin in Artemia franciscana measured by ELISA and immunohistochemistry Journal Article Fish and Shellfish Immunology, 11 , pp. 15 – 22, 2001. @article{bergh_uptake_2001, title = {Uptake and processing of a Vibrio anguillarum bacterin in Artemia franciscana measured by ELISA and immunohistochemistry}, author = {Ø Bergh and L Vikanes and P Makridis and J Skjermo and D Knappskog and O M Rødseth}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035221650&partnerID=40&md5=00a741f967e4ad5935deb0eccaf166a6}, year = {2001}, date = {2001-01-01}, journal = {Fish and Shellfish Immunology}, volume = {11}, pages = {15 -- 22}, abstract = {Nauplii of Artemia franciscana were incubated in two different concentrations (undiluted and 1:9 in autoclaved sea water) of a divalent bacterin composed of two different serovars of Vibrio anguillarum. In order to investigate uptake and further processing of a bacterin in the live feed organism A. franciscana, immunohistochemistry was applied, visualising the presence of whole bacterial cells and antigens from the bacterin in individual nauplii. By using ELISA, it was shown that approximately 1·5-2·5×105cells were incorporated into each Artemia under the conditions used. Maximum incorporation of cells was measured after 30min, whereas after 60min there was a decline to levels of 0·9-1·6×105cells per Artemia. Immediately after incubation in the bacterin solution, the nauplii were transferred to a culture of the alga Isochrysis galbana, in order to simulate transfer of the nauplii to rearing tanks for fish larvae. From the ELISA, it could be concluded that the incorporated bacterial cells were excreted from the Artemia nauplii rapidly, however a large variation among different nauplii could be visualised by immunohistochemistry. © 2001 Academic Press.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Nauplii of Artemia franciscana were incubated in two different concentrations (undiluted and 1:9 in autoclaved sea water) of a divalent bacterin composed of two different serovars of Vibrio anguillarum. In order to investigate uptake and further processing of a bacterin in the live feed organism A. franciscana, immunohistochemistry was applied, visualising the presence of whole bacterial cells and antigens from the bacterin in individual nauplii. By using ELISA, it was shown that approximately 1·5-2·5×105cells were incorporated into each Artemia under the conditions used. Maximum incorporation of cells was measured after 30min, whereas after 60min there was a decline to levels of 0·9-1·6×105cells per Artemia. Immediately after incubation in the bacterin solution, the nauplii were transferred to a culture of the alga Isochrysis galbana, in order to simulate transfer of the nauplii to rearing tanks for fish larvae. From the ELISA, it could be concluded that the incorporated bacterial cells were excreted from the Artemia nauplii rapidly, however a large variation among different nauplii could be visualised by immunohistochemistry. © 2001 Academic Press. |
2000 |
Ali, Ben Z; Boursot, P; Said, K; Lagnel, J; Chatti, N; Navajas, M Comparison of Ribosomal ITS Regions Among Androctonus spp. Scorpions (Scorpionida: Buthidae) from Tunisia Journal Article Journal of Medical Entomology, 37 (6), pp. 787–790, 2000, ISSN: 0022-2585, 1938-2928. @article{ben_ali_comparison_2000, title = {Comparison of Ribosomal ITS Regions Among Androctonus spp. Scorpions (Scorpionida: Buthidae) from Tunisia}, author = {Z Ben Ali and P Boursot and K Said and J Lagnel and N Chatti and M Navajas}, url = {https://academic.oup.com/jme/article-lookup/doi/10.1603/0022-2585-37.6.787}, doi = {10.1603/0022-2585-37.6.787}, issn = {0022-2585, 1938-2928}, year = {2000}, date = {2000-11-01}, urldate = {2020-08-17}, journal = {Journal of Medical Entomology}, volume = {37}, number = {6}, pages = {787--790}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Karakassis, I Impact of cage farming of fish on the seabed in three Mediterranean coastal areas Journal Article ICES Journal of Marine Science, 57 (5), pp. 1462–1471, 2000, ISSN: 10543139. @article{karakassis_impact_2000, title = {Impact of cage farming of fish on the seabed in three Mediterranean coastal areas}, author = {I Karakassis}, url = {https://academic.oup.com/icesjms/article-lookup/doi/10.1006/jmsc.2000.0925}, doi = {10.1006/jmsc.2000.0925}, issn = {10543139}, year = {2000}, date = {2000-10-01}, urldate = {2020-08-18}, journal = {ICES Journal of Marine Science}, volume = {57}, number = {5}, pages = {1462--1471}, abstract = {The impact of cage culture of marine fish on the benthic environment was investigated seasonally at three commercial fish farms (Cephalonia, Ithaki, and Sounion) established in coastal waters 20-30 m deep, with different types of sediment (from 80% silt to coarse sand) and varying current intensity. A transect of stations in the vicinity of the cages and a control station in each area were sampled for macrofauna and geochemical variables during July, October, and April. Redox potential in the sediment decreased under and near the cages but reached negative values only at the silty sediment site. Organic carbon and nitrogen content of the sediment near the cages increased by a factor of 1.5-5 and ATP content by 4-28 compared with the control. Azoic zones were not encountered, but the macrofaunal community was affected up to 25 m from the edge of the cages. At the coarse sediment sites, abundance and biomass under the cages were 10 times higher than at the control. Diversity indicated that the ecotone was in the vicinity of 25 m from the cages in all cases. Capitella cf. capitata dominated macrofauna up to 10 m from the cages in two fish farms, whereas the third was dominated by Protodorvillea kefersteini. Similar patterns of succession from the impacted to the normal zones were found, although macrofaunal composition differed among sites. Seasonal variability in geochemistry and macrofauna was higher in proximity of the cages. The results indicate that impacts of fish farming on benthos in the Mediterranean vary considerably depending on site characteristics. © 2000 International Council for the Exploration of the Sea.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The impact of cage culture of marine fish on the benthic environment was investigated seasonally at three commercial fish farms (Cephalonia, Ithaki, and Sounion) established in coastal waters 20-30 m deep, with different types of sediment (from 80% silt to coarse sand) and varying current intensity. A transect of stations in the vicinity of the cages and a control station in each area were sampled for macrofauna and geochemical variables during July, October, and April. Redox potential in the sediment decreased under and near the cages but reached negative values only at the silty sediment site. Organic carbon and nitrogen content of the sediment near the cages increased by a factor of 1.5-5 and ATP content by 4-28 compared with the control. Azoic zones were not encountered, but the macrofaunal community was affected up to 25 m from the edge of the cages. At the coarse sediment sites, abundance and biomass under the cages were 10 times higher than at the control. Diversity indicated that the ecotone was in the vicinity of 25 m from the cages in all cases. Capitella cf. capitata dominated macrofauna up to 10 m from the cages in two fish farms, whereas the third was dominated by Protodorvillea kefersteini. Similar patterns of succession from the impacted to the normal zones were found, although macrofaunal composition differed among sites. Seasonal variability in geochemistry and macrofauna was higher in proximity of the cages. The results indicate that impacts of fish farming on benthos in the Mediterranean vary considerably depending on site characteristics. © 2000 International Council for the Exploration of the Sea. |
Henry, Morgane A; Secombes, Christopher J The A-layer influences the susceptibility of Aeromonas salmonicida to antibacterial peptides Journal Article Fish & Shellfish Immunology, 10 (7), pp. 637–642, 2000, ISSN: 10504648. @article{henry_-layer_2000, title = {The A-layer influences the susceptibility of Aeromonas salmonicida to antibacterial peptides}, author = {Morgane A Henry and Christopher J Secombes}, url = {https://linkinghub.elsevier.com/retrieve/pii/S1050464800902882}, doi = {10.1006/fsim.2000.0288}, issn = {10504648}, year = {2000}, date = {2000-10-01}, urldate = {2020-08-18}, journal = {Fish & Shellfish Immunology}, volume = {10}, number = {7}, pages = {637--642}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Tselepides, Anastasios; Papadopoulou, Konstantia-N; Podaras, Dimitris; Plaiti, Wanda; Koutsoubas, Drosos Macrobenthic community structure over the continental margin of Crete (South Aegean Sea, NE Mediterranean) Journal Article Progress in Oceanography, 46 (2-4), pp. 401–428, 2000, ISSN: 00796611. @article{tselepides_macrobenthic_2000, title = {Macrobenthic community structure over the continental margin of Crete (South Aegean Sea, NE Mediterranean)}, author = {Anastasios Tselepides and Konstantia-N Papadopoulou and Dimitris Podaras and Wanda Plaiti and Drosos Koutsoubas}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0079661100000276}, doi = {10.1016/S0079-6611(00)00027-6}, issn = {00796611}, year = {2000}, date = {2000-08-01}, urldate = {2020-08-11}, journal = {Progress in Oceanography}, volume = {46}, number = {2-4}, pages = {401--428}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Perrot-Minnot, Marie-Jeanne; Lagnel, Jacques; Migeon, Alain; Navajas, Maria Heredity, 84 (6), pp. 702–709, 2000, ISSN: 0018-067X, 1365-2540. @article{perrot-minnot_tracking_2000, title = {Tracking paternal genes with DALP markers in a pseudoarrhenotokous reproductive system: biparental transmission but haplodiploid-like inheritance in the mite Neoseiulus californicus}, author = {Marie-Jeanne Perrot-Minnot and Jacques Lagnel and Alain Migeon and Maria Navajas}, url = {http://link.springer.com/10.1046/j.1365-2540.2000.00708.x}, doi = {10.1046/j.1365-2540.2000.00708.x}, issn = {0018-067X, 1365-2540}, year = {2000}, date = {2000-06-01}, urldate = {2020-08-18}, journal = {Heredity}, volume = {84}, number = {6}, pages = {702--709}, abstract = {The complexity of some sexual reproductive systems in arthropods still leaves both their genetic and epigenetic determinism and their evolutionary significance poorly understood. Pseudoarrhenotoky is characterized by obligate fertilization and differential inactivation and/or elimination of paternal chromosomes in embryos that develop into males. Here, we investigate how the paternal genome is transmitted in a pseudoarrhenotokous mite, Neoseiulus californicus, using codominant genetic markers detected by DALP (direct amplification of length polymorphism). Transmission patterns of parental alleles through one and two generations are reported at four or five loci corresponding to four linkage groups. Our data provide strong evidence for selective elimination of the paternal genome among male tissues. Sperm contained maternal genes exclusively, whereas some male somatic tissues retained most if not all paternal chromosomes. No recombination between parental genomes prior to paternal genome elimination from the embryonic germ line was observed. These data allow a reinterpretation of previous phenotypic and cytogenetic observations in these mites, from which we suggest some relevant mechanistic and evolutionary implications. In addition, this is the first published study using polymorphic codominant loci detected by the recently developed DALP method.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The complexity of some sexual reproductive systems in arthropods still leaves both their genetic and epigenetic determinism and their evolutionary significance poorly understood. Pseudoarrhenotoky is characterized by obligate fertilization and differential inactivation and/or elimination of paternal chromosomes in embryos that develop into males. Here, we investigate how the paternal genome is transmitted in a pseudoarrhenotokous mite, Neoseiulus californicus, using codominant genetic markers detected by DALP (direct amplification of length polymorphism). Transmission patterns of parental alleles through one and two generations are reported at four or five loci corresponding to four linkage groups. Our data provide strong evidence for selective elimination of the paternal genome among male tissues. Sperm contained maternal genes exclusively, whereas some male somatic tissues retained most if not all paternal chromosomes. No recombination between parental genomes prior to paternal genome elimination from the embryonic germ line was observed. These data allow a reinterpretation of previous phenotypic and cytogenetic observations in these mites, from which we suggest some relevant mechanistic and evolutionary implications. In addition, this is the first published study using polymorphic codominant loci detected by the recently developed DALP method. |
Makridis, Pavlos; Fjellheim, Anders Jon; Skjermo, Jorunn; Vadstein, Olav Control of the bacterial flora of Brachionus plicatilis and Artemia franciscana by incubation in bacterial suspensions Journal Article Aquaculture, 185 (3-4), pp. 207–218, 2000, ISSN: 00448486. @article{makridis_control_2000, title = {Control of the bacterial flora of Brachionus plicatilis and Artemia franciscana by incubation in bacterial suspensions}, author = {Pavlos Makridis and Anders Jon Fjellheim and Jorunn Skjermo and Olav Vadstein}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848699003518}, doi = {10.1016/S0044-8486(99)00351-8}, issn = {00448486}, year = {2000}, date = {2000-05-01}, urldate = {2020-08-17}, journal = {Aquaculture}, volume = {185}, number = {3-4}, pages = {207--218}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Dounas, C; Arvanitidis, C; Koulouri, P Artificial reefs as a management tool for the delineation, protection and enhancement of coastal ecosystems Journal Article Fishing News, 228 , pp. 94–96 (In Greek), 2000. @article{dounas_artificial_2000, title = {Artificial reefs as a management tool for the delineation, protection and enhancement of coastal ecosystems}, author = {C Dounas and C Arvanitidis and P Koulouri}, year = {2000}, date = {2000-01-01}, journal = {Fishing News}, volume = {228}, pages = {94--96 (In Greek)}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Makridis, P; Fjellheim, Jon A; Skjermo, J; Vadstein, O Colonization of the gut in first feeding turbot by bacterial strains added to the water or bioencapsulated in rotifers Journal Article Aquaculture International, 8 , pp. 367 – 380, 2000. @article{makridis_colonization_2000, title = {Colonization of the gut in first feeding turbot by bacterial strains added to the water or bioencapsulated in rotifers}, author = {P Makridis and A Jon Fjellheim and J Skjermo and O Vadstein}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034526864&partnerID=40&md5=dd669b9377094049f452d8b00dac0ab5}, year = {2000}, date = {2000-01-01}, journal = {Aquaculture International}, volume = {8}, pages = {367 -- 380}, abstract = {Two bacterial strains, 4:44 and PB52, isolated from turbot (Scophthalmus maximus L.) were used during a first feeding experiment with turbot larvae. Bacteria were either added directly to the water on the day of hatching of the larvae (day 0), or bioencapsulated in rotifers (Brachionus plicarilis) distributed on day 2 after hatching. The two bacterial strains were found to be present in the water of the rearing tanks throughout the experiment. The addition of bacteria influenced the species-composition of the microflora associated with intensively produced rotifers added to the tanks, and resulted in colonization of the gut of the larvae by the added bacterial strains. The strain 4:44 showed a peak on day 9 after hatching, and reached a mean(sem) of 2.5(1.4) x 10 4 bacteria per larva, when added in a mixture with PB52. and 0.4(0.1) x 10 4 bacteria per larva, when added alone. The strain PB52, on day 12 after hatching, reached 5.2(1.5) x 10 4 bacteria per larva when added in a mixture with strain 4:44, and 12.5(0.7) x 10 4 bacteria per larva, when added alone. The added bacteria colonized the gut turbot larvae, whereas the bacterial land, the survival and growth of the larvae were in most cases not influenced in a negative way by the addition of bacteria.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Two bacterial strains, 4:44 and PB52, isolated from turbot (Scophthalmus maximus L.) were used during a first feeding experiment with turbot larvae. Bacteria were either added directly to the water on the day of hatching of the larvae (day 0), or bioencapsulated in rotifers (Brachionus plicarilis) distributed on day 2 after hatching. The two bacterial strains were found to be present in the water of the rearing tanks throughout the experiment. The addition of bacteria influenced the species-composition of the microflora associated with intensively produced rotifers added to the tanks, and resulted in colonization of the gut of the larvae by the added bacterial strains. The strain 4:44 showed a peak on day 9 after hatching, and reached a mean(sem) of 2.5(1.4) x 10 4 bacteria per larva, when added in a mixture with PB52. and 0.4(0.1) x 10 4 bacteria per larva, when added alone. The strain PB52, on day 12 after hatching, reached 5.2(1.5) x 10 4 bacteria per larva when added in a mixture with strain 4:44, and 12.5(0.7) x 10 4 bacteria per larva, when added alone. The added bacteria colonized the gut turbot larvae, whereas the bacterial land, the survival and growth of the larvae were in most cases not influenced in a negative way by the addition of bacteria. |
Koutsoubas, D; Arvanitidis, C; Dounas, C; Drummond, L Community structure and dynamics of the Molluscan Fauna in a Mediterranean lagoon (Gialova lagoon, SW Greece) Journal Article Belgian Journal of Zoology, 130 (SUPPL. 1), pp. 131–138, 2000, ISSN: 07776276. @article{koutsoubas_community_2000, title = {Community structure and dynamics of the Molluscan Fauna in a Mediterranean lagoon (Gialova lagoon, SW Greece)}, author = {D Koutsoubas and C Arvanitidis and C Dounas and L Drummond}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-78649411533&partnerID=40&md5=0db45fbd7fa9f9b765b6564d3c978855}, issn = {07776276}, year = {2000}, date = {2000-01-01}, journal = {Belgian Journal of Zoology}, volume = {130}, number = {SUPPL. 1}, pages = {131--138}, abstract = {The molluscan community of a shallow Mediterranean lagoon (Gialova lagoon, SW Greece, Ionian Sea) was studied on a seasonal basis during 1995-1996. A total of 23 species were recorded from the lagoon. Two of them namely: Placida viridis and Polycerella emertoni are reported for the first time from the Greek seas and the Eastern Mediterranean respectively. The dominant species were the gastropods Bittium reticulatum, Cerithium vulgatum, Pirenella conica, Hydrobia acuta, Cyclope neritea and the bivalves Cerastoderma glaucum, Abra ovata. Uni- and multivariate methods were employed to study the community structure and dynamics. On the basis of the multivariate pattern of the molluscan community a coenocline is observed which is strongly related with the degree of isolation. Two main zones with different faunal composition can be distinguished: one narrow zone close to the channel of communication with the sea and another in the innermost part of the lagoon. Following the confinement scale suggested for lagoonal ecosystems by GUELORGET & PERTHUISOT (1992) the first zone can be assigned to the "zone III" and the second to "zones IVV". The distribution pattern of the molluscan community is governed by a different set of environmental variables in each season, discussed in detail. Although no disturbance due to anthropogenic impact was revealed, this narrow lagoonal habitat was proved to suffer severe "dystrophic episodes", during late summer and the beginning of autumn, but successfully recovered, demonstrating a seasonal community pattern.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The molluscan community of a shallow Mediterranean lagoon (Gialova lagoon, SW Greece, Ionian Sea) was studied on a seasonal basis during 1995-1996. A total of 23 species were recorded from the lagoon. Two of them namely: Placida viridis and Polycerella emertoni are reported for the first time from the Greek seas and the Eastern Mediterranean respectively. The dominant species were the gastropods Bittium reticulatum, Cerithium vulgatum, Pirenella conica, Hydrobia acuta, Cyclope neritea and the bivalves Cerastoderma glaucum, Abra ovata. Uni- and multivariate methods were employed to study the community structure and dynamics. On the basis of the multivariate pattern of the molluscan community a coenocline is observed which is strongly related with the degree of isolation. Two main zones with different faunal composition can be distinguished: one narrow zone close to the channel of communication with the sea and another in the innermost part of the lagoon. Following the confinement scale suggested for lagoonal ecosystems by GUELORGET & PERTHUISOT (1992) the first zone can be assigned to the "zone III" and the second to "zones IVV". The distribution pattern of the molluscan community is governed by a different set of environmental variables in each season, discussed in detail. Although no disturbance due to anthropogenic impact was revealed, this narrow lagoonal habitat was proved to suffer severe "dystrophic episodes", during late summer and the beginning of autumn, but successfully recovered, demonstrating a seasonal community pattern. |
Magoulas, A; Kotoulas, G; Gérard, A; Naciri-Graven, Y; Dermitzakis, E; Hawkins, A J S Comparison of genetic variability and parentage in different ploidy classes of the Japanese oyster Crassostrea gigas Journal Article Genetical Research, 76 (3), pp. 261–272, 2000, ISSN: 00166723. @article{magoulas_comparison_2000, title = {Comparison of genetic variability and parentage in different ploidy classes of the Japanese oyster Crassostrea gigas}, author = {A Magoulas and G Kotoulas and A Gérard and Y Naciri-Graven and E Dermitzakis and A J S Hawkins}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034520835&doi=10.1017%2fS0016672300004778&partnerID=40&md5=6f9a177082cb2cf8620b0bf2ba31d383}, doi = {10.1017/S0016672300004778}, issn = {00166723}, year = {2000}, date = {2000-01-01}, journal = {Genetical Research}, volume = {76}, number = {3}, pages = {261--272}, abstract = {Chemical treatments with cytochalasin B were used to induce triploidy in the progeny of a mass fertilization of 3 male and 7 female Crassostrea gigas parents. Triploids were produced either by retention of the first (meiosis I (MI) triploids) or the second (meiosis II (MII) triploids) polar bodies. These animals, together with their diploid siblings, were divided for two experiments. One set was used to compare physiological performance, and the other set deployed to compare growth in two different natural environments. For both experiments, genetic variability in different ploidy classes was estimated using three microsatellite loci and eight allozyme loci. The microsatellite loci were highly polymorphic, allowing independent confirmation of ploidy status and the unambiguous identification of parentage for each oyster. Significant differences in parentage were found between ploidy classes, despite the fact they originated from the same mass fertilization. This indicates that the assumptions of a common genetic background among random samples of animals taken from the same mass fertilization may be generally valid. Knowledge of parentage also allowed the more accurate scoring of allozyme loci. As expected, triploids were found to be significantly more polymorphic than diploids. However, MI triploids were not significantly more polymorphic than MII triploids. MII triploid genotypes were used to estimate recombination rates between loci and their centromeres. These rates varied between 0.29 and 0.71, indicating only moderate chiasma interference.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Chemical treatments with cytochalasin B were used to induce triploidy in the progeny of a mass fertilization of 3 male and 7 female Crassostrea gigas parents. Triploids were produced either by retention of the first (meiosis I (MI) triploids) or the second (meiosis II (MII) triploids) polar bodies. These animals, together with their diploid siblings, were divided for two experiments. One set was used to compare physiological performance, and the other set deployed to compare growth in two different natural environments. For both experiments, genetic variability in different ploidy classes was estimated using three microsatellite loci and eight allozyme loci. The microsatellite loci were highly polymorphic, allowing independent confirmation of ploidy status and the unambiguous identification of parentage for each oyster. Significant differences in parentage were found between ploidy classes, despite the fact they originated from the same mass fertilization. This indicates that the assumptions of a common genetic background among random samples of animals taken from the same mass fertilization may be generally valid. Knowledge of parentage also allowed the more accurate scoring of allozyme loci. As expected, triploids were found to be significantly more polymorphic than diploids. However, MI triploids were not significantly more polymorphic than MII triploids. MII triploid genotypes were used to estimate recombination rates between loci and their centromeres. These rates varied between 0.29 and 0.71, indicating only moderate chiasma interference. |
Koukouras, A; Dounas, C Decapod crustaceans new to the fauna of the Aegean sea Journal Article Crustaceana, 73 (4), pp. 497–502, 2000, ISSN: 0011216X. @article{koukouras_decapod_2000, title = {Decapod crustaceans new to the fauna of the Aegean sea}, author = {A Koukouras and C Dounas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034487839&doi=10.1163%2f156854000504462&partnerID=40&md5=1bf21e323555907fd4a71a1a95c1fcb8}, doi = {10.1163/156854000504462}, issn = {0011216X}, year = {2000}, date = {2000-01-01}, journal = {Crustaceana}, volume = {73}, number = {4}, pages = {497--502}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Koumoundouros, G; Divanach, P; Kentouri, M Development of the skull in Dentex dentex (Osteichthyes: Sparidae) Journal Article Marine Biology, 136 (1), pp. 175–184, 2000, ISSN: 0025-3162, 1432-1793. @article{koumoundouros_development_2000, title = {Development of the skull in Dentex dentex (Osteichthyes: Sparidae)}, author = {G Koumoundouros and P Divanach and M Kentouri}, url = {http://link.springer.com/10.1007/s002270050020}, doi = {10.1007/s002270050020}, issn = {0025-3162, 1432-1793}, year = {2000}, date = {2000-01-01}, urldate = {2020-08-17}, journal = {Marine Biology}, volume = {136}, number = {1}, pages = {175--184}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Moschou, E A; Lasarte, Azpiroz U; Fouskaki, M; Chaniotakis, N A; Papandroulakis, N; Divanach, P Direct electrochemical flow analysis system for simultaneous monitoring of total ammonia and nitrite in seawater Journal Article Aquacultural Engineering, 22 , pp. 255 – 268, 2000. @article{moschou_direct_2000, title = {Direct electrochemical flow analysis system for simultaneous monitoring of total ammonia and nitrite in seawater}, author = {E A Moschou and U Azpiroz Lasarte and M Fouskaki and N A Chaniotakis and N Papandroulakis and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034128510&partnerID=40&md5=b82b0b1ee44766fb1f6d6e77b70ec3a9}, year = {2000}, date = {2000-01-01}, journal = {Aquacultural Engineering}, volume = {22}, pages = {255 -- 268}, abstract = {The design and the performance of a new portable flow analysis (FA) system for the continuous, fast and accurate measurement of total ammonia and nitrite content in non-filtered seawater samples is demonstrated. The complete system has been optimized to operate within the ammonia and nitrite concentration range of 0.05-10 ppm. The system offers good reproducibility (textless 5%) and stability (textless 0.02 ppm/h) at constant temperature, while the analysis time is in the order of 1.5-4 min depending on the sample analyzed. The analysis results of seawater samples obtained with the FA system were compared to that obtained with the standard colorimetric method and establish the suitability of the analyzer for the precise and continuous measurements of untreated samples for both in field and laboratory applications. In addition, its small size and weight offer the advantage of portability, while its datalogging capabilities also allow for independent ammonia and nitrite monitoring. (C) 2000 Elsevier Science B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The design and the performance of a new portable flow analysis (FA) system for the continuous, fast and accurate measurement of total ammonia and nitrite content in non-filtered seawater samples is demonstrated. The complete system has been optimized to operate within the ammonia and nitrite concentration range of 0.05-10 ppm. The system offers good reproducibility (textless 5%) and stability (textless 0.02 ppm/h) at constant temperature, while the analysis time is in the order of 1.5-4 min depending on the sample analyzed. The analysis results of seawater samples obtained with the FA system were compared to that obtained with the standard colorimetric method and establish the suitability of the analyzer for the precise and continuous measurements of untreated samples for both in field and laboratory applications. In addition, its small size and weight offer the advantage of portability, while its datalogging capabilities also allow for independent ammonia and nitrite monitoring. (C) 2000 Elsevier Science B.V. |
Koumoundouros, G; Divanach, P; Savaki, A; Kentouri, M Effects of three preservation methods on the evolution of swimbladder radiographic appearance in sea bass and sea bream juveniles Journal Article Aquaculture, 182 , pp. 17 – 25, 2000. @article{koumoundouros_effects_2000, title = {Effects of three preservation methods on the evolution of swimbladder radiographic appearance in sea bass and sea bream juveniles}, author = {G Koumoundouros and P Divanach and A Savaki and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034142216&partnerID=40&md5=676bd624fd0f9054b964b990f7787846}, doi = {https://doi.org/10.1016/S0044-8486(99)00249-5}, year = {2000}, date = {2000-01-01}, journal = {Aquaculture}, volume = {182}, pages = {17 -- 25}, abstract = {Sea bass and sea bream juveniles with functional swimbladder were exposed to three methods of preservation: (a) in 10% phosphate buffered formalin, (b) at 4°C, and (c) at -25°C. They were then X-rayed daily over 10 days to evaluate the impact of these methods on the evolution of the radiographic image. All methods significantly decreased the apparent length and depth of the swimbladder, but with different intensities. Freezing had the least effect (9% and 12% reduction in bream and bass, respectively) and only during the first day of preservation. Four days of formalin fixation led to the total disappearance of this image. Preservation at 4°C led to the autolysis and putrefaction of the internal organs which was responsible for the poor images after three days. The results were discussed in terms of their use for the assessment of Juvenile quality by radiography.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Sea bass and sea bream juveniles with functional swimbladder were exposed to three methods of preservation: (a) in 10% phosphate buffered formalin, (b) at 4°C, and (c) at -25°C. They were then X-rayed daily over 10 days to evaluate the impact of these methods on the evolution of the radiographic image. All methods significantly decreased the apparent length and depth of the swimbladder, but with different intensities. Freezing had the least effect (9% and 12% reduction in bream and bass, respectively) and only during the first day of preservation. Four days of formalin fixation led to the total disappearance of this image. Preservation at 4°C led to the autolysis and putrefaction of the internal organs which was responsible for the poor images after three days. The results were discussed in terms of their use for the assessment of Juvenile quality by radiography. |
Pavlidis, M; Koumoundouros, G; Sterioti, A; Somarakis, S; Divanach, P; Kentouri, M Evidence of temperature-dependent sex determination in the european sea bass (Dicentrarchus labrax L.) Journal Article Journal of Experimental Zoology, 287 , pp. 225 – 232, 2000. @article{pavlidis_evidence_2000, title = {Evidence of temperature-dependent sex determination in the european sea bass (Dicentrarchus labrax L.)}, author = {M Pavlidis and G Koumoundouros and A Sterioti and S Somarakis and P Divanach and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034255757&partnerID=40&md5=2d194d1edd776c20c4fe6c96e412b855}, year = {2000}, date = {2000-01-01}, journal = {Journal of Experimental Zoology}, volume = {287}, pages = {225 -- 232}, abstract = {To test the hypothesis that sex determination in the European sea bass (Dicentrarchus labrax L.) can be affected by the incubating temperature during the very early developmental stages, eggs from the same batch of spontaneously spawned broodstock were divided at the stage of half-epiboly into three groups according to rearing temperature: G13 = 13°C}, keywords = {}, pubstate = {published}, tppubtype = {article} } To test the hypothesis that sex determination in the European sea bass (Dicentrarchus labrax L.) can be affected by the incubating temperature during the very early developmental stages, eggs from the same batch of spontaneously spawned broodstock were divided at the stage of half-epiboly into three groups according to rearing temperature: G13 = 13°C |
Papandroulakis, N; Markakis, G; Divanach, P; Kentouri, M Feeding requirements of sea bream (Sparus aurata) larvae under intensive rearing conditions: Development of a fuzzy logic controller for feeding Journal Article Aquacultural Engineering, 21 , pp. 285 – 299, 2000. @article{papandroulakis_feeding_2000, title = {Feeding requirements of sea bream (Sparus aurata) larvae under intensive rearing conditions: Development of a fuzzy logic controller for feeding}, author = {N Papandroulakis and G Markakis and P Divanach and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034008999&partnerID=40&md5=08b95efaa79a087e5c80a9df9872afc1}, year = {2000}, date = {2000-01-01}, journal = {Aquacultural Engineering}, volume = {21}, pages = {285 -- 299}, abstract = {Applied methods for larval rearing suffer from the difficulty of accurately predicting larvae quantitative feeding requirements for optimum growth. Even when known, requirements change according to the behavior of each population reared. Computer based feeding systems require tools for adapting the delivered amounts of food to the needs of the reared population. In this work the daily feeding requirements of sea bream (Sparus aurata) larvae were estimated, and based on these estimations a fuzzy logic controller (FLC) for feeding was designed and developed. Sea bream larvae were reared, in triplicate, under intensive conditions in a pilot scale rearing system for 20 days with the pseudo-green water method. Larvae reached 6.7 ± 0.6 mm (mean value and S.D.) at the end of the experimental period while the mean survival of the populations was 48.0 ± 12.6%. Consumption was 0.017 ± 0.009 mg of carbon per individual at first feeding and increased to 0.198 ± 0.032 mg at day 20 post-hatching. The findings were used for the development of a FLC for feeding that adapts the amount of food delivered to the larvae according to changes in the reared population. The FLC utilizes five linguistic variables describing the state of the population and a rule base composed of 316 rules. The developed FLC allows the use of computer based feeding systems during rearing of early larval stages. (C) 2000 Elsevier Science B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Applied methods for larval rearing suffer from the difficulty of accurately predicting larvae quantitative feeding requirements for optimum growth. Even when known, requirements change according to the behavior of each population reared. Computer based feeding systems require tools for adapting the delivered amounts of food to the needs of the reared population. In this work the daily feeding requirements of sea bream (Sparus aurata) larvae were estimated, and based on these estimations a fuzzy logic controller (FLC) for feeding was designed and developed. Sea bream larvae were reared, in triplicate, under intensive conditions in a pilot scale rearing system for 20 days with the pseudo-green water method. Larvae reached 6.7 ± 0.6 mm (mean value and S.D.) at the end of the experimental period while the mean survival of the populations was 48.0 ± 12.6%. Consumption was 0.017 ± 0.009 mg of carbon per individual at first feeding and increased to 0.198 ± 0.032 mg at day 20 post-hatching. The findings were used for the development of a FLC for feeding that adapts the amount of food delivered to the larvae according to changes in the reared population. The FLC utilizes five linguistic variables describing the state of the population and a rule base composed of 316 rules. The developed FLC allows the use of computer based feeding systems during rearing of early larval stages. (C) 2000 Elsevier Science B.V. |
Castritsi-Catharios, ; Kotzamanis, I Fish farming design Incollection Castritsi-Catharios, J (Ed.): Current knowledge in Aquaculture, Students’ Handbook, pp. Β 1–11, University publications, Athens, 2000, (Backup Publisher: University publications). @incollection{castritsi-catharios_fish_2000, title = {Fish farming design}, author = {Castritsi-Catharios and I Kotzamanis}, editor = {J Castritsi-Catharios}, year = {2000}, date = {2000-01-01}, booktitle = {Current knowledge in Aquaculture, Students’ Handbook}, pages = {Β 1--11}, publisher = {University publications}, address = {Athens}, series = {Modern Trends in Aquaculture}, note = {Backup Publisher: University publications}, keywords = {}, pubstate = {published}, tppubtype = {incollection} } |
Navajas, M; Tsagkarakov, A; Lagnel, J; Perrot-Minnot, M -J Genetic differentiation in Tetranychus urticae (Acari: Tetranychidae): Polymorphism, host races or sibling species? Journal Article Experimental and Applied Acarology, 24 , pp. 365 – 376, 2000. @article{navajas_genetic_2000, title = {Genetic differentiation in Tetranychus urticae (Acari: Tetranychidae): Polymorphism, host races or sibling species?}, author = {M Navajas and A Tsagkarakov and J Lagnel and M -J Perrot-Minnot}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0033665460&partnerID=40&md5=2ee7e5fb6a27402605860fa294ee99eb}, year = {2000}, date = {2000-01-01}, journal = {Experimental and Applied Acarology}, volume = {24}, pages = {365 -- 376}, abstract = {Based on allozyme electrophoresis at the Pgm locus and nuclear ribosomal DNA (ITS2) sequences, we studied the genetic variation of the two-spotted spider mite Tetranychus urticae Koch collected on rose bay, Nerium oleander L. (Apocynaceae), from several localities around the Mediterranean basin. In addition, we compared these results with those of Navajas et al. (1998) and Tsagkarakou (1997) who collected from several other host plants from the Mediterranean. In the western part of this area (Spain, France, Tunisia), we found the individuals collected from rose bay to be clearly genetically differentiated from other samples. No evidence of such host-associated differentiation was detected in the eastern Mediterranean (Italy and Greece), The genetic differentiation of mites collected on rose bay was investigated further by studying the reproductive incompatibilities between populations in Greece and in France and a laboratory strain reared on bean, Phaseolus vulgaris, in France. Reciprocal crosses performed between these strains revealed variable levels of incompatibility, spanning from partial to complete reproductive isolation. In all cases incompatibility was asymmetric. We designed a test based on double-mating to establish the fertilization status of females in fully incompatible crosses. These crosses showed that the females had been inseminated, which suggests that the barrier to reproduction is not of a prezygotic behavioral nature. The data raises the question of the relative role of ecological factors (host plant) and geographical distance, in the ongoing differentiation process potentially leading to speciation.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Based on allozyme electrophoresis at the Pgm locus and nuclear ribosomal DNA (ITS2) sequences, we studied the genetic variation of the two-spotted spider mite Tetranychus urticae Koch collected on rose bay, Nerium oleander L. (Apocynaceae), from several localities around the Mediterranean basin. In addition, we compared these results with those of Navajas et al. (1998) and Tsagkarakou (1997) who collected from several other host plants from the Mediterranean. In the western part of this area (Spain, France, Tunisia), we found the individuals collected from rose bay to be clearly genetically differentiated from other samples. No evidence of such host-associated differentiation was detected in the eastern Mediterranean (Italy and Greece), The genetic differentiation of mites collected on rose bay was investigated further by studying the reproductive incompatibilities between populations in Greece and in France and a laboratory strain reared on bean, Phaseolus vulgaris, in France. Reciprocal crosses performed between these strains revealed variable levels of incompatibility, spanning from partial to complete reproductive isolation. In all cases incompatibility was asymmetric. We designed a test based on double-mating to establish the fertilization status of females in fully incompatible crosses. These crosses showed that the females had been inseminated, which suggests that the barrier to reproduction is not of a prezygotic behavioral nature. The data raises the question of the relative role of ecological factors (host plant) and geographical distance, in the ongoing differentiation process potentially leading to speciation. |
Hatziyanni, E; Tsapakis, E; Plaiti, W; Karakassis, I Impact of organic enrichment on the vertical distribution and the size of macrofauna in a coastal fish farming size. Inproceedings pp. 341–345, Chios, Greece, 2000, (Publication Title: The 6th Hellenic Symposium of Oceanography and Fisheries,). @inproceedings{hatziyanni_impact_2000, title = {Impact of organic enrichment on the vertical distribution and the size of macrofauna in a coastal fish farming size.}, author = {E Hatziyanni and E Tsapakis and W Plaiti and I Karakassis}, year = {2000}, date = {2000-01-01}, volume = {2}, pages = {341--345}, address = {Chios, Greece}, note = {Publication Title: The 6th Hellenic Symposium of Oceanography and Fisheries,}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Perrot-Minnot, Marie-Jeanne; Lagnel, Jacques; Desmarais, Eric; Navajas, Maria Experimental and Applied Acarology, 24 (10/11), pp. 795–803, 2000, ISSN: 01688162. @article{perrot-minnot_isolation_2000, title = {Isolation and characterization by direct amplification of length polymorphisms (DALP) of codominant genetic markers with mendelian inheritance in Neoseiulus californicus (Acari : Phytoseiidae)}, author = {Marie-Jeanne Perrot-Minnot and Jacques Lagnel and Eric Desmarais and Maria Navajas}, url = {http://link.springer.com/10.1023/A:1006457310697}, doi = {10.1023/A:1006457310697}, issn = {01688162}, year = {2000}, date = {2000-01-01}, urldate = {2020-08-18}, journal = {Experimental and Applied Acarology}, volume = {24}, number = {10/11}, pages = {795--803}, abstract = {We report the first application of a new method designed to isolate polymorphic loci in any organism, the direct amplification of length polymorphism. Five polymorphic loci were readily isolated in the predatory mite Neoseiulus californicus (Acari: Phytoseiidae). Two to five alleles were identified among 46 isofemale lines based on fragment size variation due to micro-deletions/insertions. Genotyping F1 and F2 progenies from controlled heterogametic crosses and backcrosses allowed to establish the Mendelian inheritance of these alleles, their codominance, and pairwise recombination rates. Nucleotidic sequence divergence due to single base substitution was also found in the flanking regions of the polymorphism. We discuss the usefulness of these markers in studies of reproductive systems as well as population genetics, in particular, in mite species where the amount of DNA or richness in microsatellites could be limiting factors in the isolation of polymorphic loci.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We report the first application of a new method designed to isolate polymorphic loci in any organism, the direct amplification of length polymorphism. Five polymorphic loci were readily isolated in the predatory mite Neoseiulus californicus (Acari: Phytoseiidae). Two to five alleles were identified among 46 isofemale lines based on fragment size variation due to micro-deletions/insertions. Genotyping F1 and F2 progenies from controlled heterogametic crosses and backcrosses allowed to establish the Mendelian inheritance of these alleles, their codominance, and pairwise recombination rates. Nucleotidic sequence divergence due to single base substitution was also found in the flanking regions of the polymorphism. We discuss the usefulness of these markers in studies of reproductive systems as well as population genetics, in particular, in mite species where the amount of DNA or richness in microsatellites could be limiting factors in the isolation of polymorphic loci. |
Koutsoubas, D; Dounas, C; Arvanitidis, C; Kornilios, S; Petihakis, G; Triantafyllou, G; Eleftheriou, A Macrobenthic community structure and disturbance assessment in Gialova Lagoon, Ionian Sea Journal Article ICES Journal of Marine Science, 57 (5), pp. 1472–1480, 2000, ISSN: 10543139, (Publisher: Academic Press). @article{koutsoubas_macrobenthic_2000, title = {Macrobenthic community structure and disturbance assessment in Gialova Lagoon, Ionian Sea}, author = {D Koutsoubas and C Dounas and C Arvanitidis and S Kornilios and G Petihakis and G Triantafyllou and A Eleftheriou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034493473&doi=10.1006%2fjmsc.2000.0905&partnerID=40&md5=ab96a84ab0a1dbac9ddf62aba60edc11}, doi = {10.1006/jmsc.2000.0905}, issn = {10543139}, year = {2000}, date = {2000-01-01}, journal = {ICES Journal of Marine Science}, volume = {57}, number = {5}, pages = {1472--1480}, abstract = {Gialova Lagoon, a coastal marine ecosystem in the Ionian Sea, suffered the impact of an oil spill incident in October 1993, leading to the extensive fish deaths when the oil tanker "Iliad" hit bottom in the entrance of the neighbouring Navarino Bay. A multidisciplinary study investigating the structure and functioning of Gialova Lagoon for the development of an integrated economic, social, and environmental management policy consistent with its conservation was carried out on a seasonal basis during 1995/1996. One of the research priorities was to investigate the structure and dynamics of the macrozoobenthic communities and assess whether these communities had recovered from the impact of the oil spill. The various methods applied (abundance/biomass comparison, distribution of species in geometric abundance and geometric size classes) revealed no detectable disturbance of the macrobenthic communities due to anthropogenic impact. However, it was proved that the lagoonal macrofauna suffered extreme natural disturbance due to a dystrophic crisis that occurred during autumn although they successfully recovered in subsequent seasons. Different sets of environmental factors were found to be highly correlated with the temporal and spatial distribution pattern of the macrobenthic communities and are discussed in detail. The coenocline observed in Gialova Lagoon appears to be strongly correlated with the degree of water exchange with adjacent marine and continental environments. © 2000 International Council for the Exploration of the Sea.}, note = {Publisher: Academic Press}, keywords = {}, pubstate = {published}, tppubtype = {article} } Gialova Lagoon, a coastal marine ecosystem in the Ionian Sea, suffered the impact of an oil spill incident in October 1993, leading to the extensive fish deaths when the oil tanker "Iliad" hit bottom in the entrance of the neighbouring Navarino Bay. A multidisciplinary study investigating the structure and functioning of Gialova Lagoon for the development of an integrated economic, social, and environmental management policy consistent with its conservation was carried out on a seasonal basis during 1995/1996. One of the research priorities was to investigate the structure and dynamics of the macrozoobenthic communities and assess whether these communities had recovered from the impact of the oil spill. The various methods applied (abundance/biomass comparison, distribution of species in geometric abundance and geometric size classes) revealed no detectable disturbance of the macrobenthic communities due to anthropogenic impact. However, it was proved that the lagoonal macrofauna suffered extreme natural disturbance due to a dystrophic crisis that occurred during autumn although they successfully recovered in subsequent seasons. Different sets of environmental factors were found to be highly correlated with the temporal and spatial distribution pattern of the macrobenthic communities and are discussed in detail. The coenocline observed in Gialova Lagoon appears to be strongly correlated with the degree of water exchange with adjacent marine and continental environments. © 2000 International Council for the Exploration of the Sea. |
Huvet, Arnaud; Lapègue, S; Magoulas, A; Boudry, P Mitochondrial and nuclear DNA phylogeography of Crassostrea angulata, the Portuguese oyster endangered in Europe Journal Article Conservation Genetics, 1 (3), pp. 251–262, 2000, ISSN: 15660621. @article{huvet_mitochondrial_2000, title = {Mitochondrial and nuclear DNA phylogeography of Crassostrea angulata, the Portuguese oyster endangered in Europe}, author = {Arnaud Huvet and S Lapègue and A Magoulas and P Boudry}, url = {http://link.springer.com/10.1023/A:1011505805923}, doi = {10.1023/A:1011505805923}, issn = {15660621}, year = {2000}, date = {2000-01-01}, urldate = {2020-08-09}, journal = {Conservation Genetics}, volume = {1}, number = {3}, pages = {251--262}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Tsigenopoulos, C S; Berrebi, P Molecular Phylogenetics and Evolution, 14 , pp. 165 – 179, 2000. @article{tsigenopoulos_molecular_2000, title = {Molecular phylogeny of north Mediterranean freshwater barbs (genus Barbus: Cyprinidae) inferred from cytochrome b sequences: Biogeographic and systematic implications}, author = {C S Tsigenopoulos and P Berrebi}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034029971&partnerID=40&md5=ace051f20e05e83cd39f80b54a10d813}, year = {2000}, date = {2000-01-01}, journal = {Molecular Phylogenetics and Evolution}, volume = {14}, pages = {165 -- 179}, abstract = {We investigated phylogenetic relationships among north Mediterranean species of the genus Barbus using sequences of the cytochrome b gene. Our results indicate that the species belong to two major clades that are consistent with those previously defined from morphological features. The first clade includes species ranging from France to the Black Sea. In this clade, there is a well-supported monophyletic group of large-sized fluvio-lacustrine barbs; however, the monophyly of the small-sized rheophilic species is not clear. The second clade comprises species found in Spain, Greece, and Asia Minor and probably represents the oldest group present in the north Mediterranean rivers. In general, there is good concordance between geography and phylogenetic relationships. These results are compared to those from previous morphological- and allozyme-based studies and demonstrate widespread discordance and polyphyly in the traditional taxonomy of the genus Barbus. This study is one of the first reporting the phylogenetic and biogeographic relationships of a genus that is widely distributed in European rivers and contains species that are a major component of the European ichthyofauna. (C) 2000 Academic Press.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We investigated phylogenetic relationships among north Mediterranean species of the genus Barbus using sequences of the cytochrome b gene. Our results indicate that the species belong to two major clades that are consistent with those previously defined from morphological features. The first clade includes species ranging from France to the Black Sea. In this clade, there is a well-supported monophyletic group of large-sized fluvio-lacustrine barbs; however, the monophyly of the small-sized rheophilic species is not clear. The second clade comprises species found in Spain, Greece, and Asia Minor and probably represents the oldest group present in the north Mediterranean rivers. In general, there is good concordance between geography and phylogenetic relationships. These results are compared to those from previous morphological- and allozyme-based studies and demonstrate widespread discordance and polyphyly in the traditional taxonomy of the genus Barbus. This study is one of the first reporting the phylogenetic and biogeographic relationships of a genus that is widely distributed in European rivers and contains species that are a major component of the European ichthyofauna. (C) 2000 Academic Press. |
Arvanitidis, C Polychaete fauna of the Aegean Sea: Inventory and new information Journal Article Bulletin of Marine Science, 66 (1), pp. 73–96, 2000, ISSN: 00074977, (Publisher: Rosenstiel School of Marine and Atmospheric Science). @article{arvanitidis_polychaete_2000, title = {Polychaete fauna of the Aegean Sea: Inventory and new information}, author = {C Arvanitidis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034580549&partnerID=40&md5=2f5e5caaa482887d362e82dfe1ece550}, issn = {00074977}, year = {2000}, date = {2000-01-01}, journal = {Bulletin of Marine Science}, volume = {66}, number = {1}, pages = {73--96}, abstract = {Examination of collections from the Aegean Sea and a review of the literature revealed that 592 polychaete species have been recorded from this area. Five of the species identified are reported for the first time from the Mediterranean and the Black Sea, 38 species are new records for the Eastern Mediterranean and 55 are new elements for the Aegean polychaete fauna. Information on the habitat and the geographic distribution of the species newly reported from the Eastern Mediterranean is given. The checklist of the Aegean Polychaeta is presented. Although being located in the vicinity of the Eastern Mediterranean province and the Black Sea which are characterized as faunistically impoverished areas, the Aegean hosts a rich polychaete fauna, second in order in species diversity after the Western Mediterranean. The latter demonstrates the importance of this area in the understanding the patterns of polychaete distribution along the Mediterranean and the Black Sea.}, note = {Publisher: Rosenstiel School of Marine and Atmospheric Science}, keywords = {}, pubstate = {published}, tppubtype = {article} } Examination of collections from the Aegean Sea and a review of the literature revealed that 592 polychaete species have been recorded from this area. Five of the species identified are reported for the first time from the Mediterranean and the Black Sea, 38 species are new records for the Eastern Mediterranean and 55 are new elements for the Aegean polychaete fauna. Information on the habitat and the geographic distribution of the species newly reported from the Eastern Mediterranean is given. The checklist of the Aegean Polychaeta is presented. Although being located in the vicinity of the Eastern Mediterranean province and the Black Sea which are characterized as faunistically impoverished areas, the Aegean hosts a rich polychaete fauna, second in order in species diversity after the Western Mediterranean. The latter demonstrates the importance of this area in the understanding the patterns of polychaete distribution along the Mediterranean and the Black Sea. |
Plaiti, W; Akoumianaki, I; Eleftheriou, A Seasonal and bathymetric trends of the amphipod fauna in Heraklion Bay (Aegean Sea) Journal Article Polskie Archiwum Hydrobiologii, 47 , pp. 517 – 525, 2000. @article{plaiti_seasonal_2000, title = {Seasonal and bathymetric trends of the amphipod fauna in Heraklion Bay (Aegean Sea)}, author = {W Plaiti and I Akoumianaki and A Eleftheriou}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034430130&partnerID=40&md5=6f5edad5cfd6e473fac2226dc4071988}, year = {2000}, date = {2000-01-01}, journal = {Polskie Archiwum Hydrobiologii}, volume = {47}, pages = {517 -- 525}, abstract = {The distribution of the benthic amphipod fauna in Heraklion Bay along a bathymetric gradient from 70 to 160 m was investigated during 1988-89 and 1991-92 by means of quantitative grab sampling. A total of 1456 individuals belonging to 38 species was found in 305 grab samples. Differences in the distribution of the dominant species as well in the structure of the amphipod community, as evidenced by cluster analysis, indicate the presence of distinct environmental conditions between the upper and lower circalittoral zone of Heraklion Bay. Temporal variability although evident does not clearly correspond to expected seasonal trends.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The distribution of the benthic amphipod fauna in Heraklion Bay along a bathymetric gradient from 70 to 160 m was investigated during 1988-89 and 1991-92 by means of quantitative grab sampling. A total of 1456 individuals belonging to 38 species was found in 305 grab samples. Differences in the distribution of the dominant species as well in the structure of the amphipod community, as evidenced by cluster analysis, indicate the presence of distinct environmental conditions between the upper and lower circalittoral zone of Heraklion Bay. Temporal variability although evident does not clearly correspond to expected seasonal trends. |
Pavlidis, M; Greenwood, L; Mourot, B; Kokkari, C; Menn, Le F; Divanach, P; Scott, A P General and Comparative Endocrinology, 118 , pp. 14 – 25, 2000. @article{pavlidis_seasonal_2000, title = {Seasonal variations and maturity stages in relation to differences in serum levels of gonadal steroids, vitellogenin, and thyroid hormones in the common dentex (Dentex dentex)}, author = {M Pavlidis and L Greenwood and B Mourot and C Kokkari and F Le Menn and P Divanach and A P Scott}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034006188&partnerID=40&md5=d5286b0db60ae52af7841e4963700b70}, year = {2000}, date = {2000-01-01}, journal = {General and Comparative Endocrinology}, volume = {118}, pages = {14 -- 25}, abstract = {Seasonal variations in serum concentrations of 17β-estradiol (E2), vitellogenin (Vg), testosterone (T), 11 ketotestosterone (11-KT), and thyroid hormones (T4, L-thyroxine; and T3, 3,5,3’-triiodo-L-thyronine) were investigated during the first, second, and third reproductive cycles in intensively reared populations of common dentex, Dentex dentex, and correlated with gonadal development and spawning. In females, there were baseline E2 values (<0.10 ng/ml) and negligible Vg concentrations during the postspawning and pregametogenesis period (June to December), and these increased thereafter to peak during the spawning period. Maximum T3 and T4 serum concentrations were found around spawning. There was a positive correlation during vitellogenesis and final maturation between Vg and T3 (r2 = 0.366). In addition, Vg and T3 concentrations were statistically higher in the stages of vitellogenesis and final maturation than at the other stages (P < 0.001). Minimum T3 and T4 concentrations (October) coincided with the decrease in water temperature and the associated decrease in the daily feeding rate and the specific growth rate. In males, as in females, seasonal changes in serum levels of T and 11-KT were well correlated with gonadal development. The presence of males in the stage of completed spermiogenesis in December coincided with the surge in both androgens and this increase lasted until the end of the spawning period. There were no significant differences in serum T3 and T4 levels among the maturity stages. The observed seasonal changes in serum gonadal steroids and Vg reflected the pattern of oocyte development and the spawning behavior of common dentex and were typical of the patterns described in most multiple spawners studied to date. Thyroid hormones may enhance early ovarian development and stimulate vitellogenesis in female dentex. (C) 2000 Academic Press.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Seasonal variations in serum concentrations of 17β-estradiol (E2), vitellogenin (Vg), testosterone (T), 11 ketotestosterone (11-KT), and thyroid hormones (T4, L-thyroxine; and T3, 3,5,3’-triiodo-L-thyronine) were investigated during the first, second, and third reproductive cycles in intensively reared populations of common dentex, Dentex dentex, and correlated with gonadal development and spawning. In females, there were baseline E2 values (<0.10 ng/ml) and negligible Vg concentrations during the postspawning and pregametogenesis period (June to December), and these increased thereafter to peak during the spawning period. Maximum T3 and T4 serum concentrations were found around spawning. There was a positive correlation during vitellogenesis and final maturation between Vg and T3 (r2 = 0.366). In addition, Vg and T3 concentrations were statistically higher in the stages of vitellogenesis and final maturation than at the other stages (P < 0.001). Minimum T3 and T4 concentrations (October) coincided with the decrease in water temperature and the associated decrease in the daily feeding rate and the specific growth rate. In males, as in females, seasonal changes in serum levels of T and 11-KT were well correlated with gonadal development. The presence of males in the stage of completed spermiogenesis in December coincided with the surge in both androgens and this increase lasted until the end of the spawning period. There were no significant differences in serum T3 and T4 levels among the maturity stages. The observed seasonal changes in serum gonadal steroids and Vg reflected the pattern of oocyte development and the spawning behavior of common dentex and were typical of the patterns described in most multiple spawners studied to date. Thyroid hormones may enhance early ovarian development and stimulate vitellogenesis in female dentex. (C) 2000 Academic Press. |
Hawkins, A J S; Magoulas, A; Héral, M; Bougrier, S; Naciri-Graven, Y; Day, A J; Kotoulas, G Genetical Research, 76 (3), pp. 273–284, 2000, ISSN: 00166723. @article{hawkins_separate_2000, title = {Separate effects of triploidy, parentage and genomic diversity upon feeding behaviour, metabolic efficiency and net energy balance in the pacific oyster Crassostrea gigas}, author = {A J S Hawkins and A Magoulas and M Héral and S Bougrier and Y Naciri-Graven and A J Day and G Kotoulas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034521887&doi=10.1017%2fS0016672300004766&partnerID=40&md5=b1e5a6ea52adb13f396dcec5d01c96d9}, doi = {10.1017/S0016672300004766}, issn = {00166723}, year = {2000}, date = {2000-01-01}, journal = {Genetical Research}, volume = {76}, number = {3}, pages = {273--284}, abstract = {Triploid oysters were induced using cytochalasin B upon retention of either the first (meiosis I triploids) or the second (meiosis II triploids) polar body in embryos from a single cohort derived from mixed parentage. Allozyme and microsatellite assays enabled the confirmation of both parentage and triploidy status in each oyster. Comparison of meiosis I triploids, meiosis II triploids and diploid siblings established that improved physiological performance in triploids was associated with increased allelic variation, rather than with the quantitative dosage effects of ploidy status. An unidentified maternal influence also interacted with genotype. Among full sibs, allelic variation measured as multi-locus enzyme heterozygosity accounted for up to 42% of the variance in physiological performance; significant positive influences were identified upon feeding rate, absorption efficiency, net energy balance and growth efficiency (= net energy balance ÷ energy absorbed). Whilst allelic variation was greater in both meiosis I and meiosis II triploids than in diploid siblings, both allelic variation and net energy balance were highest in triploids induced at meiosis I. This suggests that it may be preferable to induce triploidy by blocking meiosis I, rather than meiosis II as has traditionally been undertaken during commercial breeding programmes.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Triploid oysters were induced using cytochalasin B upon retention of either the first (meiosis I triploids) or the second (meiosis II triploids) polar body in embryos from a single cohort derived from mixed parentage. Allozyme and microsatellite assays enabled the confirmation of both parentage and triploidy status in each oyster. Comparison of meiosis I triploids, meiosis II triploids and diploid siblings established that improved physiological performance in triploids was associated with increased allelic variation, rather than with the quantitative dosage effects of ploidy status. An unidentified maternal influence also interacted with genotype. Among full sibs, allelic variation measured as multi-locus enzyme heterozygosity accounted for up to 42% of the variance in physiological performance; significant positive influences were identified upon feeding rate, absorption efficiency, net energy balance and growth efficiency (= net energy balance ÷ energy absorbed). Whilst allelic variation was greater in both meiosis I and meiosis II triploids than in diploid siblings, both allelic variation and net energy balance were highest in triploids induced at meiosis I. This suggests that it may be preferable to induce triploidy by blocking meiosis I, rather than meiosis II as has traditionally been undertaken during commercial breeding programmes. |
Lampadariou, N; Karakassis, Ioannis; Chapdelaine, L; Wilkinson, M F; Dafnomili, E Spatial variability in water column and sediment chemistry in Heraklion harbour (Crete, E. Mediterranean Journal Article Fresenius Envir Bull, 9 , pp. 164–171, 2000. @article{lampadariou_spatial_2000, title = {Spatial variability in water column and sediment chemistry in Heraklion harbour (Crete, E. Mediterranean}, author = {N Lampadariou and Ioannis Karakassis and L Chapdelaine and M F Wilkinson and E Dafnomili}, year = {2000}, date = {2000-01-01}, journal = {Fresenius Envir Bull}, volume = {9}, pages = {164--171}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Athanassopoulou, F; Cotou, E; Watsos, I; Giagnisi, M Study of the bacteriostatic activity of an Artemia enrichment compound based on plant extracts from Angelica sp Journal Article Bulletin of the Hellenic Veterinary Medical Society, 51 , pp. 293–296, 2000. @article{athanassopoulou_study_2000, title = {Study of the bacteriostatic activity of an Artemia enrichment compound based on plant extracts from Angelica sp}, author = {F Athanassopoulou and E Cotou and I Watsos and M Giagnisi}, year = {2000}, date = {2000-01-01}, journal = {Bulletin of the Hellenic Veterinary Medical Society}, volume = {51}, pages = {293--296}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Triantafyllou, G; Petihakis, G; Dounas, C; Koutsoubas, D; Arvanitidis, C; Eleftheriou, A Temporal variations in benthic communities and their response to physicochemical forcing: A numerical approach Journal Article ICES Journal of Marine Science, 57 (5), pp. 1507–1516, 2000, ISSN: 10543139, (Publisher: Academic Press). @article{triantafyllou_temporal_2000, title = {Temporal variations in benthic communities and their response to physicochemical forcing: A numerical approach}, author = {G Triantafyllou and G Petihakis and C Dounas and D Koutsoubas and C Arvanitidis and A Eleftheriou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034492083&doi=10.1006%2fjmsc.2000.0923&partnerID=40&md5=37dbe9cbf95e8c4e2e62b7f6e7c163d2}, doi = {10.1006/jmsc.2000.0923}, issn = {10543139}, year = {2000}, date = {2000-01-01}, journal = {ICES Journal of Marine Science}, volume = {57}, number = {5}, pages = {1507--1516}, abstract = {A generic complex ecological model, the European Regional Seas Ecosystem Model (ERSEM), was applied to a shallow lagoon system in the Eastern Mediterranean. Model results depicting the seasonal variation of nutrients and Chl-α in the water column, as well as three benthic functional groups (suspended feeders, deposit feeders, and benthic carnivores), are validated with in situ data. The likely effect of a technical intervention (river input) increasing the freshwater nutrient inputs on ecosystem functioning is also investigated. Detailed annual carbon fluxes and benthic fauna biomasses are calculated, before and after the river input. The importance of external physical/chemical forcing on the pelagic system and its subsequent effect on the benthic system are demonstrated. Model experiments indicate the shift of the ecosystem from nitrate limitation to predator control with external inputs. Model experiments also show a significant increase in the amount of carbon entering the benthic system through the activity of filter feeders when river inputs are implemented. © 2000 International Council for the Exploration of the Sea.}, note = {Publisher: Academic Press}, keywords = {}, pubstate = {published}, tppubtype = {article} } A generic complex ecological model, the European Regional Seas Ecosystem Model (ERSEM), was applied to a shallow lagoon system in the Eastern Mediterranean. Model results depicting the seasonal variation of nutrients and Chl-α in the water column, as well as three benthic functional groups (suspended feeders, deposit feeders, and benthic carnivores), are validated with in situ data. The likely effect of a technical intervention (river input) increasing the freshwater nutrient inputs on ecosystem functioning is also investigated. Detailed annual carbon fluxes and benthic fauna biomasses are calculated, before and after the river input. The importance of external physical/chemical forcing on the pelagic system and its subsequent effect on the benthic system are demonstrated. Model experiments indicate the shift of the ecosystem from nitrate limitation to predator control with external inputs. Model experiments also show a significant increase in the amount of carbon entering the benthic system through the activity of filter feeders when river inputs are implemented. © 2000 International Council for the Exploration of the Sea. |
Maurizi, A; Diaz, J P; Divanach, P; Papandroulakis, N; Connes, R The effect of glycerol dissolved in the rearing water on the transition to exotrophy in gilthead sea bream Sparus aurata larvae Journal Article Aquaculture, 189 , pp. 119 – 131, 2000. @article{maurizi_effect_2000, title = {The effect of glycerol dissolved in the rearing water on the transition to exotrophy in gilthead sea bream Sparus aurata larvae}, author = {A Maurizi and J P Diaz and P Divanach and N Papandroulakis and R Connes}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034715335&partnerID=40&md5=2ad2493b26d28332d0f11b019e7a552a}, year = {2000}, date = {2000-01-01}, journal = {Aquaculture}, volume = {189}, pages = {119 -- 131}, abstract = {The effects of glycerol added to seawater at different concentrations were tested in monocultures of Chlorella minutissima and Brachionus plicatilis in mixed cultures of these two species and on starved or fed Sparus aurata larvae. Glycerol enhanced the growth of Chlorella and seemed to indirectly benefit the rotifers that feed on it. Its beneficial effect on gilthead sea bream prelarvae and larvae resulted in glycogen accumulation in the liver and absence of signs of cholestasis-like pathology. This effect varied according to the glycerol concentration in the water, to its addition timing and to light and feeding conditions. The gluconeogenic origin of the hepatic glycogen reserves is discussed, together with the mediating role of the food chain in glycerol larval assimilation. (C) 2000 Elsevier Science B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The effects of glycerol added to seawater at different concentrations were tested in monocultures of Chlorella minutissima and Brachionus plicatilis in mixed cultures of these two species and on starved or fed Sparus aurata larvae. Glycerol enhanced the growth of Chlorella and seemed to indirectly benefit the rotifers that feed on it. Its beneficial effect on gilthead sea bream prelarvae and larvae resulted in glycogen accumulation in the liver and absence of signs of cholestasis-like pathology. This effect varied according to the glycerol concentration in the water, to its addition timing and to light and feeding conditions. The gluconeogenic origin of the hepatic glycogen reserves is discussed, together with the mediating role of the food chain in glycerol larval assimilation. (C) 2000 Elsevier Science B.V. |
McArthur, V E; Koutsoubas, D; Lampadariou, N; Dounas, C The meiofaunal community structure of a Mediterranean lagoon (Gialova lagoon, Ionian Sea) Journal Article Helgoland Marine Research, 54 (1), pp. 7–17, 2000, ISSN: 1438387X, (Publisher: Springer Verlag). @article{mcarthur_meiofaunal_2000, title = {The meiofaunal community structure of a Mediterranean lagoon (Gialova lagoon, Ionian Sea)}, author = {V E McArthur and D Koutsoubas and N Lampadariou and C Dounas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034125190&doi=10.1007%2fs101520050031&partnerID=40&md5=a39dd162e646b360cd77d24fb663534d}, doi = {10.1007/s101520050031}, issn = {1438387X}, year = {2000}, date = {2000-01-01}, journal = {Helgoland Marine Research}, volume = {54}, number = {1}, pages = {7--17}, abstract = {The higher taxonomic structure of a meiofaunal community was investigated in an Eastern Mediterranean lagoon (Gialova lagoon, Ionian Sea). Seven stations were sampled on a seasonal basis during 1995-1996. Stations within the lagoon were found to support a maximum of 14 meiofaunal taxa with densities ranging from 17 to over 2000 individuals per 10 cm2. Nematodes were not always the most abundant taxa, although they dominated about half of the total 28 samples. Uni- and multivariate analyses were employed to study the community structure. The distribution pattern of the meiofaunal community varied both across the lagoon and over the seasons. On the basis of the spatial differences a meiofaunal coenocline, correlated with the degree of isolation, was observed, composed of mainly two zones: one defined by the area close to the marine channel and the other the more isolated area in the inner lagoon. Meiofaunal distribution pattern was not clearly correlated to one environmental variable, but rather to many, and spatial and seasonal effects could be seen.}, note = {Publisher: Springer Verlag}, keywords = {}, pubstate = {published}, tppubtype = {article} } The higher taxonomic structure of a meiofaunal community was investigated in an Eastern Mediterranean lagoon (Gialova lagoon, Ionian Sea). Seven stations were sampled on a seasonal basis during 1995-1996. Stations within the lagoon were found to support a maximum of 14 meiofaunal taxa with densities ranging from 17 to over 2000 individuals per 10 cm2. Nematodes were not always the most abundant taxa, although they dominated about half of the total 28 samples. Uni- and multivariate analyses were employed to study the community structure. The distribution pattern of the meiofaunal community varied both across the lagoon and over the seasons. On the basis of the spatial differences a meiofaunal coenocline, correlated with the degree of isolation, was observed, composed of mainly two zones: one defined by the area close to the marine channel and the other the more isolated area in the inner lagoon. Meiofaunal distribution pattern was not clearly correlated to one environmental variable, but rather to many, and spatial and seasonal effects could be seen. |
Rigos, G; Kotzamanis, I; Gialamas, I; Nengas, I; Alexis, M Toxicity and digestibility of fumagillin DCH in gilthead sea bream, Sparus aurata L. Journal Article Journal of Fish Diseases, 23 , pp. 161 – 164, 2000. @article{rigos_toxicity_2000, title = {Toxicity and digestibility of fumagillin DCH in gilthead sea bream, Sparus aurata L.}, author = {G Rigos and I Kotzamanis and I Gialamas and I Nengas and M Alexis}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0033835878&partnerID=40&md5=fe8776f28328e1bb9903067f107d2210}, year = {2000}, date = {2000-01-01}, journal = {Journal of Fish Diseases}, volume = {23}, pages = {161 -- 164}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
P., Katharios; Κ., Kapata-Zoumbou; Iliopoulou-Georgudaki, J Toxicity of intraperitoneal administered ivermectin in sea bream, Sparus aurata. Inproceedings of Sciences, Hellenic Society Biological (Ed.): pp. 56–60, Chios, Greece, 2000. @inproceedings{katharios_toxicity_2000, title = {Toxicity of intraperitoneal administered ivermectin in sea bream, Sparus aurata.}, author = {Katharios P. and Kapata-Zoumbou Κ. and J Iliopoulou-Georgudaki}, editor = {Hellenic Society Biological of Sciences}, year = {2000}, date = {2000-01-01}, pages = {56--60}, address = {Chios, Greece}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Mylonas, C C; Zohar, Y Use of GnRHa-delivery systems for the control of reproduction in fish Journal Article Reviews in Fish Biology and Fisheries, 10 (4), pp. 463–491, 2000, ISSN: 09603166. @article{mylonas_use_2000, title = {Use of GnRHa-delivery systems for the control of reproduction in fish}, author = {C C Mylonas and Y Zohar}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034528406&doi=10.1023%2fA%3a1012279814708&partnerID=40&md5=93dc83bb17d6f49997555b7c15c0bd30}, doi = {10.1023/A:1012279814708}, issn = {09603166}, year = {2000}, date = {2000-01-01}, journal = {Reviews in Fish Biology and Fisheries}, volume = {10}, number = {4}, pages = {463--491}, abstract = {The most commonly observed reproductive dysfunctions in cultured fish are the unpredictability of final oocyte maturation (FOM) in females, and the diminished volume and quality of sperm in males. Gonadotropin-releasing hormone agonists (GnRHa) have been used extensively in order to stimulate the release of pituitary luteinizing hormone (LH) required to induce FOM, ovulation and spermiation. Because multiple hormonal treatments are often necessary for a successful response, fish must be monitored and handled extensively, which is labor intensive, stressful to the fish and can often result in broodstock mortalities. To ameliorate this problem, sustained-release delivery systems for GnRHa have been developed during the last two decades and have been increasingly applied in controlling reproduction of a variety of cultured fish. Solid implants of cholesterol or poly[ethylene-vinyl acetate], and biodegradable microspheres of poly[lactide-glycolide] or poly[fatty acid dimer-sebasic acid] release GnRHa for a period of time (from a few days to many weeks.) GnRHa-delivery systems do not cause desensitization of the pituitary gonadotrophs in fish, and by stimulating a sustained elevation of plasma LH they induce the natural progression of plasma steroid increases associated with FOM and spermiation. This method has been used with very encouraging results in females of more than 40 cultured species and has been effective in inducing FOM, ovulation or spawning in fish with synchronous, group-synchronous and asynchronous ovarian development. In males, GnRHa-delivery systems have been tested in more than 20 species, producing significant increases in milt production for up to 5 weeks. Future research should focus on the optimization of this technology in terms of (a) using the most potent GnRHa, (b) identifying the most appropriate GnRHa release kinetics according to the reproductive biology of different species, and (c) determining minimum effective doses. Developments in these areas will greatly enhance the effectiveness and efficiency of GnRHa-delivery systems, while at the same time reducing their cost thus making them more affordable to the aquaculture industry.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The most commonly observed reproductive dysfunctions in cultured fish are the unpredictability of final oocyte maturation (FOM) in females, and the diminished volume and quality of sperm in males. Gonadotropin-releasing hormone agonists (GnRHa) have been used extensively in order to stimulate the release of pituitary luteinizing hormone (LH) required to induce FOM, ovulation and spermiation. Because multiple hormonal treatments are often necessary for a successful response, fish must be monitored and handled extensively, which is labor intensive, stressful to the fish and can often result in broodstock mortalities. To ameliorate this problem, sustained-release delivery systems for GnRHa have been developed during the last two decades and have been increasingly applied in controlling reproduction of a variety of cultured fish. Solid implants of cholesterol or poly[ethylene-vinyl acetate], and biodegradable microspheres of poly[lactide-glycolide] or poly[fatty acid dimer-sebasic acid] release GnRHa for a period of time (from a few days to many weeks.) GnRHa-delivery systems do not cause desensitization of the pituitary gonadotrophs in fish, and by stimulating a sustained elevation of plasma LH they induce the natural progression of plasma steroid increases associated with FOM and spermiation. This method has been used with very encouraging results in females of more than 40 cultured species and has been effective in inducing FOM, ovulation or spawning in fish with synchronous, group-synchronous and asynchronous ovarian development. In males, GnRHa-delivery systems have been tested in more than 20 species, producing significant increases in milt production for up to 5 weeks. Future research should focus on the optimization of this technology in terms of (a) using the most potent GnRHa, (b) identifying the most appropriate GnRHa release kinetics according to the reproductive biology of different species, and (c) determining minimum effective doses. Developments in these areas will greatly enhance the effectiveness and efficiency of GnRHa-delivery systems, while at the same time reducing their cost thus making them more affordable to the aquaculture industry. |
1999 |
Asplin, Lars; Salvanes, Anne Gro Vea; Kristoffersen, Jon Bent Nonlocal wind-driven fjord-coast advection and its potential effect on plankton and fish recruitment Journal Article Fisheries Oceanography, 8 (4), pp. 255–263, 1999, ISSN: 1054-6006, 1365-2419. @article{asplin_nonlocal_1999, title = {Nonlocal wind-driven fjord-coast advection and its potential effect on plankton and fish recruitment}, author = {Lars Asplin and Anne Gro Vea Salvanes and Jon Bent Kristoffersen}, url = {http://doi.wiley.com/10.1046/j.1365-2419.1999.00109.x}, doi = {10.1046/j.1365-2419.1999.00109.x}, issn = {1054-6006, 1365-2419}, year = {1999}, date = {1999-12-01}, urldate = {2020-08-18}, journal = {Fisheries Oceanography}, volume = {8}, number = {4}, pages = {255--263}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Koumoundouros, G; Divanach, P; Kentouri, M Ontogeny and allometric plasticity of Dentex dentex (Osteichthyes: Sparidae) in rearing conditions Journal Article Marine Biology, 135 (3), pp. 561–572, 1999, ISSN: 0025-3162, 1432-1793. @article{koumoundouros_ontogeny_1999, title = {Ontogeny and allometric plasticity of Dentex dentex (Osteichthyes: Sparidae) in rearing conditions}, author = {G Koumoundouros and P Divanach and M Kentouri}, url = {http://link.springer.com/10.1007/s002270050657}, doi = {10.1007/s002270050657}, issn = {0025-3162, 1432-1793}, year = {1999}, date = {1999-12-01}, urldate = {2020-08-18}, journal = {Marine Biology}, volume = {135}, number = {3}, pages = {561--572}, abstract = {The ontogeny of Dentex dentex (Linnaeus, 1758) was studied comparatively under extensive and semi-extensive rearing conditions. The exotrophic phase was divided into three distinct developmental stages (a) larval, from feeding onset up to 10 mm total length (TL); (b) metamorphosis, from 10 mm TL up to 24 mm TL; and (c) further juveniles. The majority of all ontogenic changes was expressed during the larval stage and integrated during the metamorphosis stage. The juvenile stage was characterized by developmental stability. The normal development of D. dentex was seriously affected by the rearing conditions applied during the early exotrophic phase. The two differently reared populations followed similar developmental patterns of general morphology, pigmentation and behaviour, but they presented high developmental plasticity where allometric growth was concerned. Rearing conditions influenced both the boundaries of the sequential stages (in 50% of the morphometric ratios) and the allometry coefficient of each morphometric character in the various developmental stages (in 80% of the characters). The results are discussed with respect to the ontogeny of the functional morphology under both ecological and aquaculture considerations.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The ontogeny of Dentex dentex (Linnaeus, 1758) was studied comparatively under extensive and semi-extensive rearing conditions. The exotrophic phase was divided into three distinct developmental stages (a) larval, from feeding onset up to 10 mm total length (TL); (b) metamorphosis, from 10 mm TL up to 24 mm TL; and (c) further juveniles. The majority of all ontogenic changes was expressed during the larval stage and integrated during the metamorphosis stage. The juvenile stage was characterized by developmental stability. The normal development of D. dentex was seriously affected by the rearing conditions applied during the early exotrophic phase. The two differently reared populations followed similar developmental patterns of general morphology, pigmentation and behaviour, but they presented high developmental plasticity where allometric growth was concerned. Rearing conditions influenced both the boundaries of the sequential stages (in 50% of the morphometric ratios) and the allometry coefficient of each morphometric character in the various developmental stages (in 80% of the characters). The results are discussed with respect to the ontogeny of the functional morphology under both ecological and aquaculture considerations. |
Kristoffersen, JB; Salvanes, AGV Growth, life history and population genetics of Benthosema glacial in fjordic and oceanic environments Inproceedings Kristoffersen, JB (Ed.): Gilleleje, Denmark, 1999. @inproceedings{kristoffersen_growth_1999, title = {Growth, life history and population genetics of Benthosema glacial in fjordic and oceanic environments}, author = {JB Kristoffersen and AGV Salvanes}, editor = {JB Kristoffersen}, year = {1999}, date = {1999-11-01}, address = {Gilleleje, Denmark}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Nengas, Ioannis; Alexis, Maria N; Davies, Simon J High inclusion levels of poultry meals and related byproducts in diets for gilthead seabream Sparus aurata L. Journal Article Aquaculture, 179 (1-4), pp. 13–23, 1999, ISSN: 00448486. @article{nengas_high_1999, title = {High inclusion levels of poultry meals and related byproducts in diets for gilthead seabream Sparus aurata L.}, author = {Ioannis Nengas and Maria N Alexis and Simon J Davies}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848699001489}, doi = {10.1016/S0044-8486(99)00148-9}, issn = {00448486}, year = {1999}, date = {1999-09-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {179}, number = {1-4}, pages = {13--23}, abstract = {The feasibility of replacing fish meal protein at high levels of 75 and 100% with a high quality poultry meat meal was assessed in diets for gilthead seabream. A combined mixture of poultry meat meal and feather meal was also tested at high inclusion levels of 75 and 100%. Finally, two lower grade poultry by-product meals produced for the Greek industry were tested at various levels for comparison. The diets were isocaloric containing 18 MJ/kg of gross energy, isonitrogenous (CP: 45%) and had a lipid content of 13% on an as fed basis. The experiment was carried out in a semiclosed rearing system and its duration was 84 days. The groups of fish fed 75 and 100% poultry meat meal showed a slight reduction in growth parameters compared to fish fed the control diet containing white fish meal but was not statistically significant (P textless 0.05). Similar results were obtained for the fish fed the poultry and feather mixture. Feed efficiency, protein and energy utilisation followed the same trends. One of the locally produced meals at substitution levels of up to 50% produced no significant reduction in growth of seabream (P textless 0.05). At the level of 75% of the protein, however, the material caused a severe decrease in growth performance, feed efficiency, protein efficiency ratio and apparent net protein utilisation. Inferior quality was also demonstrated by the other poultry meal available on the Greek market. Protein digestibility coefficients were measured for all diets and essential amino acid indices and chemical score values were calculated.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The feasibility of replacing fish meal protein at high levels of 75 and 100% with a high quality poultry meat meal was assessed in diets for gilthead seabream. A combined mixture of poultry meat meal and feather meal was also tested at high inclusion levels of 75 and 100%. Finally, two lower grade poultry by-product meals produced for the Greek industry were tested at various levels for comparison. The diets were isocaloric containing 18 MJ/kg of gross energy, isonitrogenous (CP: 45%) and had a lipid content of 13% on an as fed basis. The experiment was carried out in a semiclosed rearing system and its duration was 84 days. The groups of fish fed 75 and 100% poultry meat meal showed a slight reduction in growth parameters compared to fish fed the control diet containing white fish meal but was not statistically significant (P textless 0.05). Similar results were obtained for the fish fed the poultry and feather mixture. Feed efficiency, protein and energy utilisation followed the same trends. One of the locally produced meals at substitution levels of up to 50% produced no significant reduction in growth of seabream (P textless 0.05). At the level of 75% of the protein, however, the material caused a severe decrease in growth performance, feed efficiency, protein efficiency ratio and apparent net protein utilisation. Inferior quality was also demonstrated by the other poultry meal available on the Greek market. Protein digestibility coefficients were measured for all diets and essential amino acid indices and chemical score values were calculated. |
Alexis, Maria N; Nengas, Ioannis; Fountoulaki, Eleni; Papoutsi, Eleni; Andriopoulou, Argiro; Koutsodimou, Maria; Gaubaudan, J Aquaculture, 179 (1-4), pp. 447–456, 1999, ISSN: 00448486. @article{alexis_tissue_1999, title = {Tissue ascorbic acid levels in European sea bass (Dicentrarchus labrax) and gilthead sea bream (Sparus aurata L.) fingerlings fed diets containing different forms of ascorbic acid}, author = {Maria N Alexis and Ioannis Nengas and Eleni Fountoulaki and Eleni Papoutsi and Argiro Andriopoulou and Maria Koutsodimou and J Gaubaudan}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848699001787}, doi = {10.1016/S0044-8486(99)00178-7}, issn = {00448486}, year = {1999}, date = {1999-09-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {179}, number = {1-4}, pages = {447--456}, abstract = {Sea bass and sea bream fingerlings of 3-4 g initial weight were fed four diets supplemented with 250 mg/kg of ascorbic acid (AA) supplied as the fat coated form or different phosphate ester forms. An ascorbate free diet was also fed. Before the start of the experiment, fish were fed an ascorbate free diet in order to deplete their ascorbate stores. The same diet formulation was used in all treatments containing fish meal LT, soybean meal and wheat flour as the main ingredients. Feeding levels were the same for all groups of fish and were close to satiation. The results obtained for fish growth and feed efficiency did not differ significantly among groups of fish for both species studied. Muscle, liver and kidney were analysed for AA content after frequent sampling of fish. Tissue ascorbate levels increased after feeding the ascorbate containing diets, the rate of increase was although higher for sea bream than for sea bass. Ascorbate levels in groups fed the fat coated diet were lower than those fed the diets containing phosphate esters, probably due to a higher destruction of this form during feed preparation and storage. AA levels in the tissues studied were different with muscle having the lowest and kidney the highest value. Ascorbate levels in fish fed the ascorbate free diet decreased significantly. No growth reduction or deficiency signs were observed.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Sea bass and sea bream fingerlings of 3-4 g initial weight were fed four diets supplemented with 250 mg/kg of ascorbic acid (AA) supplied as the fat coated form or different phosphate ester forms. An ascorbate free diet was also fed. Before the start of the experiment, fish were fed an ascorbate free diet in order to deplete their ascorbate stores. The same diet formulation was used in all treatments containing fish meal LT, soybean meal and wheat flour as the main ingredients. Feeding levels were the same for all groups of fish and were close to satiation. The results obtained for fish growth and feed efficiency did not differ significantly among groups of fish for both species studied. Muscle, liver and kidney were analysed for AA content after frequent sampling of fish. Tissue ascorbate levels increased after feeding the ascorbate containing diets, the rate of increase was although higher for sea bream than for sea bass. Ascorbate levels in groups fed the fat coated diet were lower than those fed the diets containing phosphate esters, probably due to a higher destruction of this form during feed preparation and storage. AA levels in the tissues studied were different with muscle having the lowest and kidney the highest value. Ascorbate levels in fish fed the ascorbate free diet decreased significantly. No growth reduction or deficiency signs were observed. |
Rufingier, Catherine; Pasteur, Nicole; Lagnel, Jacques; Martin, Christian; Navajas, Maria Mechanisms of insecticide resistance in the aphid Nasonovia ribisnigri (Mosley) (Homoptera: Aphididae) from France Journal Article Insect Biochemistry and Molecular Biology, 29 (4), pp. 385–391, 1999, ISSN: 09651748. @article{rufingier_mechanisms_1999, title = {Mechanisms of insecticide resistance in the aphid Nasonovia ribisnigri (Mosley) (Homoptera: Aphididae) from France}, author = {Catherine Rufingier and Nicole Pasteur and Jacques Lagnel and Christian Martin and Maria Navajas}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0965174899000144}, doi = {10.1016/S0965-1748(99)00014-4}, issn = {09651748}, year = {1999}, date = {1999-04-01}, urldate = {2020-08-18}, journal = {Insect Biochemistry and Molecular Biology}, volume = {29}, number = {4}, pages = {385--391}, abstract = {Nasonovia ribisnigri, a main pest of salad crops, has developed resistance to Various insecticides in southern France, including the carbamate pirimicarb and the cyclodiene endosulfan, two insecticides widely used to control this aphid. Here we have investigated the mechanisms of resistance to these two insecticides by studying cross-resistance, synergism, activity of detoxifying enzymes, and possible modifications of the target proteins. Resistance to pirimicarb was shown to be mainly due to a decreased sensitivity of the target acetylcholinesterase; this modification confered also, resistance to propoxur but not to methomyl and the two tested organophosphates (acephate and paraoxon). Endosulfan resistance was associated with a moderate level of resistance to dieldrin, and resistance to both insecticides was due, in part, to increased detoxification by glutathione S-transferases (GST). The endosulfan resistant strain displayed the same amino acid at position 302 of the Rdl gene (GABA receptor) as susceptible aphids (e.g. Ala), indicating that the Ala to Ser (or to Gly) mutation observed among dieldrin resistant strains of other insect species was not present. (C) 1999 Elsevier Science Ltd. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Nasonovia ribisnigri, a main pest of salad crops, has developed resistance to Various insecticides in southern France, including the carbamate pirimicarb and the cyclodiene endosulfan, two insecticides widely used to control this aphid. Here we have investigated the mechanisms of resistance to these two insecticides by studying cross-resistance, synergism, activity of detoxifying enzymes, and possible modifications of the target proteins. Resistance to pirimicarb was shown to be mainly due to a decreased sensitivity of the target acetylcholinesterase; this modification confered also, resistance to propoxur but not to methomyl and the two tested organophosphates (acephate and paraoxon). Endosulfan resistance was associated with a moderate level of resistance to dieldrin, and resistance to both insecticides was due, in part, to increased detoxification by glutathione S-transferases (GST). The endosulfan resistant strain displayed the same amino acid at position 302 of the Rdl gene (GABA receptor) as susceptible aphids (e.g. Ala), indicating that the Ala to Ser (or to Gly) mutation observed among dieldrin resistant strains of other insect species was not present. (C) 1999 Elsevier Science Ltd. All rights reserved. |
Makridis, Pavlos; Olsen, Yngvar Protein depletion of the rotifer Brachionus plicatilis during starvation Journal Article Aquaculture, 174 (3-4), pp. 343–353, 1999, ISSN: 00448486. @article{makridis_protein_1999, title = {Protein depletion of the rotifer Brachionus plicatilis during starvation}, author = {Pavlos Makridis and Yngvar Olsen}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848699000204}, doi = {10.1016/S0044-8486(99)00020-4}, issn = {00448486}, year = {1999}, date = {1999-04-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {174}, number = {3-4}, pages = {343--353}, abstract = {During the cultivation of marine fish larvae, unconsumed rotifers (Brachionus plicatilis) may reside in the tanks for several days and their nutritional value may become severely reduced. In order to quantify this process, body biomass and protein content per individual rotifer was measured during starvation at different temperatures (4-28°C) for a period of 5 to 7 days. Both well-fed rotifers and poorly-fed rotifers were used, in order to examine the effect of the nutritional status at the start of the experiment on the loss rates of biomass and protein. At high temperatures the carbon, nitrogen and amino acid content decreased exponentially with time. A large part of body biomass (40-50%) was lost during the first 4 days of starvation at 18-20°C. The rate of decrease was positively related to the temperature. Poorly-fed rotifers were more sensitive to starvation than well-fed rotifers, as their nitrogen content decreased at a higher rate. Starvation of rotifers may be prevented by addition of microalgae in concentrations of 1-2 mg C l-1. At 4-5°C, the carbon, nitrogen and amino acid content remained relatively stable. Rotifers may thus be stored for < 5 days at 4-5°C without a significant loss in their nutritional value.}, keywords = {}, pubstate = {published}, tppubtype = {article} } During the cultivation of marine fish larvae, unconsumed rotifers (Brachionus plicatilis) may reside in the tanks for several days and their nutritional value may become severely reduced. In order to quantify this process, body biomass and protein content per individual rotifer was measured during starvation at different temperatures (4-28°C) for a period of 5 to 7 days. Both well-fed rotifers and poorly-fed rotifers were used, in order to examine the effect of the nutritional status at the start of the experiment on the loss rates of biomass and protein. At high temperatures the carbon, nitrogen and amino acid content decreased exponentially with time. A large part of body biomass (40-50%) was lost during the first 4 days of starvation at 18-20°C. The rate of decrease was positively related to the temperature. Poorly-fed rotifers were more sensitive to starvation than well-fed rotifers, as their nitrogen content decreased at a higher rate. Starvation of rotifers may be prevented by addition of microalgae in concentrations of 1-2 mg C l-1. At 4-5°C, the carbon, nitrogen and amino acid content remained relatively stable. Rotifers may thus be stored for < 5 days at 4-5°C without a significant loss in their nutritional value. |
Navajas, M; Gutierrez, J; Lagnel, J; Fauvel, G; Gotoh, T Entomologia Experimentalis Et Applicata, 90 (2), pp. 113–122, 1999, (ISBN: 0013-8703). @article{navajas_dna_1999, title = {DNA sequences and cross-breeding experiments in the hawthorn spider mite Amphitetranychus viennensis reveal high genetic differentiation between Japanese and French populations}, author = {M Navajas and J Gutierrez and J Lagnel and G Fauvel and T Gotoh}, url = {<Go to ISI>://000080275700001}, year = {1999}, date = {1999-02-01}, journal = {Entomologia Experimentalis Et Applicata}, volume = {90}, number = {2}, pages = {113--122}, abstract = {Sequence variation of the complete second internal transcribed spacer (ITS2, 445 bp) of nuclear ribosomal DNA and part of the mitochondrial cytochrome oxidase I gene (COI, 350 bp) was examined in Amphitetranychus viennensis (Zacher) mites (Acari:Tetranychidae) from four French and four Japanese locations. Sequence analysis consistently revealed the separation of the samples in two major groups: French mites differed from Japanese by 3.8-4.1% of the nucleotide divergence in COI sequences. These two groups also displayed distinct ITS2 consensus sequences (2.1% nucleotide divergence). A few variations, not affecting the diagnostic sites around the consensus sequence, were revealed among cloned copies of the same individual. Reciprocal crosses and backcrosses between one French and two Japanese populations disclosed strong reproductive incompatibility. However, fertile hybrid females were obtained, indicating the conspecificity of the tested mites. Despite the presence of Wolbachia in the French strain, but not in the Japanese ones, our crosses did not display the unidirectional incompatibility typically produced by this microorganism, but rather a bidirectional - although asymmetrical - incompatibility pattern. The post-zygotic incompatibilities in A. viennensis cannot be explained by the presence of Wolbachia but to some extent by mite genome divergence resulting from limited gene exchange between allopatric populations. Experiments of Wolbachia elimination by antibiotic treatment and subsequent crosses with cured strains are still needed to fully understand the reproductive incompatibility patterns in this mite species.}, note = {ISBN: 0013-8703}, keywords = {}, pubstate = {published}, tppubtype = {article} } Sequence variation of the complete second internal transcribed spacer (ITS2, 445 bp) of nuclear ribosomal DNA and part of the mitochondrial cytochrome oxidase I gene (COI, 350 bp) was examined in Amphitetranychus viennensis (Zacher) mites (Acari:Tetranychidae) from four French and four Japanese locations. Sequence analysis consistently revealed the separation of the samples in two major groups: French mites differed from Japanese by 3.8-4.1% of the nucleotide divergence in COI sequences. These two groups also displayed distinct ITS2 consensus sequences (2.1% nucleotide divergence). A few variations, not affecting the diagnostic sites around the consensus sequence, were revealed among cloned copies of the same individual. Reciprocal crosses and backcrosses between one French and two Japanese populations disclosed strong reproductive incompatibility. However, fertile hybrid females were obtained, indicating the conspecificity of the tested mites. Despite the presence of Wolbachia in the French strain, but not in the Japanese ones, our crosses did not display the unidirectional incompatibility typically produced by this microorganism, but rather a bidirectional - although asymmetrical - incompatibility pattern. The post-zygotic incompatibilities in A. viennensis cannot be explained by the presence of Wolbachia but to some extent by mite genome divergence resulting from limited gene exchange between allopatric populations. Experiments of Wolbachia elimination by antibiotic treatment and subsequent crosses with cured strains are still needed to fully understand the reproductive incompatibility patterns in this mite species. |
Koumoundouros, G; Divanach, P; Kentouri, M Osteological development of the vertebral column and of the caudal complex in Dentex dentex Journal Article Journal of Fish Biology, 54 (2), pp. 424–436, 1999, ISSN: 0022-1112, 1095-8649. @article{koumoundouros_osteological_1999, title = {Osteological development of the vertebral column and of the caudal complex in Dentex dentex}, author = {G Koumoundouros and P Divanach and M Kentouri}, url = {http://doi.wiley.com/10.1111/j.1095-8649.1999.tb00841.x}, doi = {10.1111/j.1095-8649.1999.tb00841.x}, issn = {0022-1112, 1095-8649}, year = {1999}, date = {1999-02-01}, urldate = {2020-08-18}, journal = {Journal of Fish Biology}, volume = {54}, number = {2}, pages = {424--436}, abstract = {Osteological development of the vertebral column and caudal complex in common dentex was described under extensive larval rearing conditions. Generally, the cartilaginous bones developed prior to the membranous bones. The development of the axial skeleton began with the formation of the hypural 1, the neural arches 2 and 3, as well as the haemal arches 1-8 at 4.8, 4.9 and 5.0 mm total length (L(T), measured in vivo), respectively. By 7.5 mm L(T), all the cartilaginous elements were formed, except for the ventral ribs, which formed between the range of 8.4-18.0 mm L(T) The caudal lepidotrichia were the first membranous bones to appear (5.3 mm L(T)) and attain their full meristic count (7.4 mm L(T)), followed by the vertebral centra, which formed between 6.6 and 9.7 mm L(T). By 25.0 mm L(T), all the elements were fully ossified except for the ventral ribs. The developmental direction and order of all the elements were studied with respect to their formation and ossification. The results were discussed in the contexts of ichthyoplankton, ecology and aquaculture. Compared with other Sparidae species, common dentex followed a pattern of relatively rapid rate of osteological development.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Osteological development of the vertebral column and caudal complex in common dentex was described under extensive larval rearing conditions. Generally, the cartilaginous bones developed prior to the membranous bones. The development of the axial skeleton began with the formation of the hypural 1, the neural arches 2 and 3, as well as the haemal arches 1-8 at 4.8, 4.9 and 5.0 mm total length (L(T), measured in vivo), respectively. By 7.5 mm L(T), all the cartilaginous elements were formed, except for the ventral ribs, which formed between the range of 8.4-18.0 mm L(T) The caudal lepidotrichia were the first membranous bones to appear (5.3 mm L(T)) and attain their full meristic count (7.4 mm L(T)), followed by the vertebral centra, which formed between 6.6 and 9.7 mm L(T). By 25.0 mm L(T), all the elements were fully ossified except for the ventral ribs. The developmental direction and order of all the elements were studied with respect to their formation and ossification. The results were discussed in the contexts of ichthyoplankton, ecology and aquaculture. Compared with other Sparidae species, common dentex followed a pattern of relatively rapid rate of osteological development. |
Publications
2001 |
The colonization of experimental artificial reefs, from the serpulids polychaetes, in the N. Aegean Sea (Greece). preliminary results Inproceedings Rapp. Comm. Int. Mer Médit., pp. 352, Monaco, 2001. |
Aquaculture International, 9 , pp. 247 – 253, 2001. |
The Poseidon operational tool for the prediction of floating pollutant transport Journal Article Marine Pollution Bulletin, 43 , pp. 270–278, 2001. |
The pseudo-green water technique for intensive rearing of sea bream (Sparus aurata) larvae Journal Article Aquaculture International, 9 , pp. 205 – 216, 2001. |
Toxicity of intraperitoneally injected ivermectin in sea bream, Sparus aurata Journal Article Fish Physiology and Biochemistry, 25 (2), pp. 99–108, 2001, ISSN: 09201742. |
Uptake and processing of a Vibrio anguillarum bacterin in Artemia franciscana measured by ELISA and immunohistochemistry Journal Article Fish and Shellfish Immunology, 11 , pp. 15 – 22, 2001. |
2000 |
Comparison of Ribosomal ITS Regions Among Androctonus spp. Scorpions (Scorpionida: Buthidae) from Tunisia Journal Article Journal of Medical Entomology, 37 (6), pp. 787–790, 2000, ISSN: 0022-2585, 1938-2928. |
Impact of cage farming of fish on the seabed in three Mediterranean coastal areas Journal Article ICES Journal of Marine Science, 57 (5), pp. 1462–1471, 2000, ISSN: 10543139. |
The A-layer influences the susceptibility of Aeromonas salmonicida to antibacterial peptides Journal Article Fish & Shellfish Immunology, 10 (7), pp. 637–642, 2000, ISSN: 10504648. |
Macrobenthic community structure over the continental margin of Crete (South Aegean Sea, NE Mediterranean) Journal Article Progress in Oceanography, 46 (2-4), pp. 401–428, 2000, ISSN: 00796611. |
Heredity, 84 (6), pp. 702–709, 2000, ISSN: 0018-067X, 1365-2540. |
Control of the bacterial flora of Brachionus plicatilis and Artemia franciscana by incubation in bacterial suspensions Journal Article Aquaculture, 185 (3-4), pp. 207–218, 2000, ISSN: 00448486. |
Artificial reefs as a management tool for the delineation, protection and enhancement of coastal ecosystems Journal Article Fishing News, 228 , pp. 94–96 (In Greek), 2000. |
Colonization of the gut in first feeding turbot by bacterial strains added to the water or bioencapsulated in rotifers Journal Article Aquaculture International, 8 , pp. 367 – 380, 2000. |
Community structure and dynamics of the Molluscan Fauna in a Mediterranean lagoon (Gialova lagoon, SW Greece) Journal Article Belgian Journal of Zoology, 130 (SUPPL. 1), pp. 131–138, 2000, ISSN: 07776276. |
Comparison of genetic variability and parentage in different ploidy classes of the Japanese oyster Crassostrea gigas Journal Article Genetical Research, 76 (3), pp. 261–272, 2000, ISSN: 00166723. |
Decapod crustaceans new to the fauna of the Aegean sea Journal Article Crustaceana, 73 (4), pp. 497–502, 2000, ISSN: 0011216X. |
Development of the skull in Dentex dentex (Osteichthyes: Sparidae) Journal Article Marine Biology, 136 (1), pp. 175–184, 2000, ISSN: 0025-3162, 1432-1793. |
Direct electrochemical flow analysis system for simultaneous monitoring of total ammonia and nitrite in seawater Journal Article Aquacultural Engineering, 22 , pp. 255 – 268, 2000. |
Effects of three preservation methods on the evolution of swimbladder radiographic appearance in sea bass and sea bream juveniles Journal Article Aquaculture, 182 , pp. 17 – 25, 2000. |
Evidence of temperature-dependent sex determination in the european sea bass (Dicentrarchus labrax L.) Journal Article Journal of Experimental Zoology, 287 , pp. 225 – 232, 2000. |
Feeding requirements of sea bream (Sparus aurata) larvae under intensive rearing conditions: Development of a fuzzy logic controller for feeding Journal Article Aquacultural Engineering, 21 , pp. 285 – 299, 2000. |
Fish farming design Incollection Castritsi-Catharios, J (Ed.): Current knowledge in Aquaculture, Students’ Handbook, pp. Β 1–11, University publications, Athens, 2000, (Backup Publisher: University publications). |
Genetic differentiation in Tetranychus urticae (Acari: Tetranychidae): Polymorphism, host races or sibling species? Journal Article Experimental and Applied Acarology, 24 , pp. 365 – 376, 2000. |
Impact of organic enrichment on the vertical distribution and the size of macrofauna in a coastal fish farming size. Inproceedings pp. 341–345, Chios, Greece, 2000, (Publication Title: The 6th Hellenic Symposium of Oceanography and Fisheries,). |
Experimental and Applied Acarology, 24 (10/11), pp. 795–803, 2000, ISSN: 01688162. |
Macrobenthic community structure and disturbance assessment in Gialova Lagoon, Ionian Sea Journal Article ICES Journal of Marine Science, 57 (5), pp. 1472–1480, 2000, ISSN: 10543139, (Publisher: Academic Press). |
Mitochondrial and nuclear DNA phylogeography of Crassostrea angulata, the Portuguese oyster endangered in Europe Journal Article Conservation Genetics, 1 (3), pp. 251–262, 2000, ISSN: 15660621. |
Molecular Phylogenetics and Evolution, 14 , pp. 165 – 179, 2000. |
Polychaete fauna of the Aegean Sea: Inventory and new information Journal Article Bulletin of Marine Science, 66 (1), pp. 73–96, 2000, ISSN: 00074977, (Publisher: Rosenstiel School of Marine and Atmospheric Science). |
Seasonal and bathymetric trends of the amphipod fauna in Heraklion Bay (Aegean Sea) Journal Article Polskie Archiwum Hydrobiologii, 47 , pp. 517 – 525, 2000. |
General and Comparative Endocrinology, 118 , pp. 14 – 25, 2000. |
Genetical Research, 76 (3), pp. 273–284, 2000, ISSN: 00166723. |
Spatial variability in water column and sediment chemistry in Heraklion harbour (Crete, E. Mediterranean Journal Article Fresenius Envir Bull, 9 , pp. 164–171, 2000. |
Study of the bacteriostatic activity of an Artemia enrichment compound based on plant extracts from Angelica sp Journal Article Bulletin of the Hellenic Veterinary Medical Society, 51 , pp. 293–296, 2000. |
Temporal variations in benthic communities and their response to physicochemical forcing: A numerical approach Journal Article ICES Journal of Marine Science, 57 (5), pp. 1507–1516, 2000, ISSN: 10543139, (Publisher: Academic Press). |
The effect of glycerol dissolved in the rearing water on the transition to exotrophy in gilthead sea bream Sparus aurata larvae Journal Article Aquaculture, 189 , pp. 119 – 131, 2000. |
The meiofaunal community structure of a Mediterranean lagoon (Gialova lagoon, Ionian Sea) Journal Article Helgoland Marine Research, 54 (1), pp. 7–17, 2000, ISSN: 1438387X, (Publisher: Springer Verlag). |
Toxicity and digestibility of fumagillin DCH in gilthead sea bream, Sparus aurata L. Journal Article Journal of Fish Diseases, 23 , pp. 161 – 164, 2000. |
Toxicity of intraperitoneal administered ivermectin in sea bream, Sparus aurata. Inproceedings of Sciences, Hellenic Society Biological (Ed.): pp. 56–60, Chios, Greece, 2000. |
Use of GnRHa-delivery systems for the control of reproduction in fish Journal Article Reviews in Fish Biology and Fisheries, 10 (4), pp. 463–491, 2000, ISSN: 09603166. |
1999 |
Nonlocal wind-driven fjord-coast advection and its potential effect on plankton and fish recruitment Journal Article Fisheries Oceanography, 8 (4), pp. 255–263, 1999, ISSN: 1054-6006, 1365-2419. |
Ontogeny and allometric plasticity of Dentex dentex (Osteichthyes: Sparidae) in rearing conditions Journal Article Marine Biology, 135 (3), pp. 561–572, 1999, ISSN: 0025-3162, 1432-1793. |
Growth, life history and population genetics of Benthosema glacial in fjordic and oceanic environments Inproceedings Kristoffersen, JB (Ed.): Gilleleje, Denmark, 1999. |
High inclusion levels of poultry meals and related byproducts in diets for gilthead seabream Sparus aurata L. Journal Article Aquaculture, 179 (1-4), pp. 13–23, 1999, ISSN: 00448486. |
Aquaculture, 179 (1-4), pp. 447–456, 1999, ISSN: 00448486. |
Mechanisms of insecticide resistance in the aphid Nasonovia ribisnigri (Mosley) (Homoptera: Aphididae) from France Journal Article Insect Biochemistry and Molecular Biology, 29 (4), pp. 385–391, 1999, ISSN: 09651748. |
Protein depletion of the rotifer Brachionus plicatilis during starvation Journal Article Aquaculture, 174 (3-4), pp. 343–353, 1999, ISSN: 00448486. |
Entomologia Experimentalis Et Applicata, 90 (2), pp. 113–122, 1999, (ISBN: 0013-8703). |
Osteological development of the vertebral column and of the caudal complex in Dentex dentex Journal Article Journal of Fish Biology, 54 (2), pp. 424–436, 1999, ISSN: 0022-1112, 1095-8649. |