1998 |
Desmarais, E; Lanneluc, I; Lagnel, J Direct amplification of length polymorphisms (DALP), or how to get and characterize new genetic markers in many species Journal Article Nucleic Acids Research, 26 (6), pp. 1458–1465, 1998, ISSN: 0305-1048, 1362-4962. @article{desmarais_direct_1998, title = {Direct amplification of length polymorphisms (DALP), or how to get and characterize new genetic markers in many species}, author = {E Desmarais and I Lanneluc and J Lagnel}, url = {https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/26.6.1458}, doi = {10.1093/nar/26.6.1458}, issn = {0305-1048, 1362-4962}, year = {1998}, date = {1998-03-01}, urldate = {2020-08-17}, journal = {Nucleic Acids Research}, volume = {26}, number = {6}, pages = {1458--1465}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Rigos, G; Christophilogiannis, P; Giahnishi, M; Andriopoulou, A; Koutsodimoy, M; Nengas, I; Alexis, M Amyloodinium occelatum infestation on sharpsnout sea bream, Puntazzo puntazzo cetti Journal Article Bulletin of the European Association of Fish Pathologists, 18 , pp. 198 – 200, 1998. @article{rigos_amyloodinium_1998, title = {Amyloodinium occelatum infestation on sharpsnout sea bream, Puntazzo puntazzo cetti}, author = {G Rigos and P Christophilogiannis and M Giahnishi and A Andriopoulou and M Koutsodimoy and I Nengas and M Alexis}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-3042877967&partnerID=40&md5=e145ac3be1044818c798bd4a72383612}, year = {1998}, date = {1998-01-01}, journal = {Bulletin of the European Association of Fish Pathologists}, volume = {18}, pages = {198 -- 200}, abstract = {The devastating effect of Amyloodinium occelatum on sharpsnout sea bream, Puntazzo puntazzo Cetti is described in this note. Massive and rapid mortalities (100% in 48 h) of cultured juvenile (12 g) sharpsnout sea bream were attributed to an epizootic infection with the dinoflagellate A. occelatum parasitising the gills. A clinical infection was also apparent in gilthead sea bream, Sparus aurata L (10 g ) kept in adjacent cages but 25 % of the infected population survived the infection. On the contrary, sea bass, Dicentrarchus labrax L. (20gr) that were maintained near to the infected Sparids remained unaffected. This epizootic was attributed to the location of the cages site and the optimum for the parasite water temperature. The incidence confirms the lethal identity of the particular pathogen on cultured fish.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The devastating effect of Amyloodinium occelatum on sharpsnout sea bream, Puntazzo puntazzo Cetti is described in this note. Massive and rapid mortalities (100% in 48 h) of cultured juvenile (12 g) sharpsnout sea bream were attributed to an epizootic infection with the dinoflagellate A. occelatum parasitising the gills. A clinical infection was also apparent in gilthead sea bream, Sparus aurata L (10 g ) kept in adjacent cages but 25 % of the infected population survived the infection. On the contrary, sea bass, Dicentrarchus labrax L. (20gr) that were maintained near to the infected Sparids remained unaffected. This epizootic was attributed to the location of the cages site and the optimum for the parasite water temperature. The incidence confirms the lethal identity of the particular pathogen on cultured fish. |
Magoulas, A Application of molecular markers to aquaculture and broodstock management with special emphasis on microsatellite DNA Journal Article Cahiers Options Mediterrannes, 34 , pp. 153– 168, 1998. @article{magoulas_application_1998, title = {Application of molecular markers to aquaculture and broodstock management with special emphasis on microsatellite DNA}, author = {A Magoulas}, year = {1998}, date = {1998-01-01}, journal = {Cahiers Options Mediterrannes}, volume = {34}, pages = {153-- 168}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Dounas, C; Koutsoubas, D; Arvanitidis, C; Petihakis, G; Drummond, L; A, Eleftheriou Biodiversity and the impact of anthropogenic activities in Mediterranean lagoons: The case of Gialova lagoon, SW Greece Journal Article Oebalia, 24 , pp. 77–91, 1998. @article{dounas_biodiversity_1998, title = {Biodiversity and the impact of anthropogenic activities in Mediterranean lagoons: The case of Gialova lagoon, SW Greece}, author = {C Dounas and D Koutsoubas and C Arvanitidis and G Petihakis and L Drummond and Eleftheriou A}, year = {1998}, date = {1998-01-01}, journal = {Oebalia}, volume = {24}, pages = {77--91}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Drakopoulos, PG; Kornilios, S; Kristallas, A; Dounas, C Distribution of floating tar in the Cretan Sea, Eastern Mediterranean, and its relation to the surface circulation Inproceedings Technical University of Crete, Chania Greece, 1998, (Publication Title: 7th International Conference on Natural & Man - Made Hazards). @inproceedings{drakopoulos_distribution_1998, title = {Distribution of floating tar in the Cretan Sea, Eastern Mediterranean, and its relation to the surface circulation}, author = {PG Drakopoulos and S Kornilios and A Kristallas and C Dounas}, year = {1998}, date = {1998-01-01}, address = {Technical University of Crete, Chania Greece}, note = {Publication Title: 7th International Conference on Natural & Man - Made Hazards}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Mylonas, C C; III, Woods L C; Thomas, P; Zohar, Y General and Comparative Endocrinology, 110 (3), pp. 276–289, 1998, ISSN: 00166480, (Publisher: Academic Press Inc.). @article{mylonas_endocrine_1998, title = {Endocrine profiles of female-striped bass (Morone saxatilis) in captivity, during postvitellogenesis and induction of final oocyte maturation via controlled-release GnRHa-delivery systems}, author = {C C Mylonas and L C Woods III and P Thomas and Y Zohar}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0031840295&doi=10.1006%2fgcen.1998.7073&partnerID=40&md5=c7317f6fe3df89e568e9b512562853c4}, doi = {10.1006/gcen.1998.7073}, issn = {00166480}, year = {1998}, date = {1998-01-01}, journal = {General and Comparative Endocrinology}, volume = {110}, number = {3}, pages = {276--289}, abstract = {Plasma levels of reproductive and thyroid hormones were measured in captive striped bass females during postvitellogenesis and the spawning period (March-June). Circulating gonadotropin II (GtH II), 17,20β-dihydroxy- 4-pregnen-3-one (17,20β-P), and 17,20β,21-trihydroxy-4-pregnen-3-one (17,20β,21-P) remained low and unchanged in nonmaturing females, while 17β- estradiol (E2) and testosterone (T) declined throughout postvitellogenesis. Plasma thyroxine (T4) declined significantly in mid-April, while triiodothyronine (T3) increased in mid-May. The only female that ovulated spontaneously had markedly different E2, T, and T3 profiles during postvitellogenesis, and had a surge in plasma GtH II during final oocyte maturation (FOM). The lack of a GtH II surge is presumably responsible for the absence of FOM, but earlier, and as of yet unknown, endocrine disruptions during postvitellogenesis may determine the female's ability to undergo FOM. Treatment of females with a gonadotropin-releasing hormone agonist (GnRHa)- delivery system induced FOM and ovulation within 3 and 10 days, respectively, and resulted in the production of fertile eggs. Plasma GtH II increased continually after GnRHa implantation, even in the presence of declining GnRHa plasma levels. Plasma E2 increased first and peaked prior to FOM, whereas T peaked at the peripheral germinal vesicle (GV) stage. Plasma 17,20β-P and 17,20β,21-P increased dramatically at the GV breakdown (GVBD) stage. Plasma T4 was unaffected by the GnRHa treatment, whereas T3 decreased after GnRHa implantation and remained low throughout FOM. Based on the observed hormonal profiles, FOM can be separated into an early phase (lipid-droplet coalescence, GV migration) associated with E2 and T elevations, and a late phase (yolk-globule coalescence, GVBD) associated with 17,20β-P and 17,20β,21-P elevation.}, note = {Publisher: Academic Press Inc.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Plasma levels of reproductive and thyroid hormones were measured in captive striped bass females during postvitellogenesis and the spawning period (March-June). Circulating gonadotropin II (GtH II), 17,20β-dihydroxy- 4-pregnen-3-one (17,20β-P), and 17,20β,21-trihydroxy-4-pregnen-3-one (17,20β,21-P) remained low and unchanged in nonmaturing females, while 17β- estradiol (E2) and testosterone (T) declined throughout postvitellogenesis. Plasma thyroxine (T4) declined significantly in mid-April, while triiodothyronine (T3) increased in mid-May. The only female that ovulated spontaneously had markedly different E2, T, and T3 profiles during postvitellogenesis, and had a surge in plasma GtH II during final oocyte maturation (FOM). The lack of a GtH II surge is presumably responsible for the absence of FOM, but earlier, and as of yet unknown, endocrine disruptions during postvitellogenesis may determine the female's ability to undergo FOM. Treatment of females with a gonadotropin-releasing hormone agonist (GnRHa)- delivery system induced FOM and ovulation within 3 and 10 days, respectively, and resulted in the production of fertile eggs. Plasma GtH II increased continually after GnRHa implantation, even in the presence of declining GnRHa plasma levels. Plasma E2 increased first and peaked prior to FOM, whereas T peaked at the peripheral germinal vesicle (GV) stage. Plasma 17,20β-P and 17,20β,21-P increased dramatically at the GV breakdown (GVBD) stage. Plasma T4 was unaffected by the GnRHa treatment, whereas T3 decreased after GnRHa implantation and remained low throughout FOM. Based on the observed hormonal profiles, FOM can be separated into an early phase (lipid-droplet coalescence, GV migration) associated with E2 and T elevations, and a late phase (yolk-globule coalescence, GVBD) associated with 17,20β-P and 17,20β,21-P elevation. |
Magoulas, A; Kotoulas, G; Batargias, C; Zouros, E Genetic markers in marine biology and aquaculture research: when to use what Incollection Agnese, J F (Ed.): Genetics and Aquaculture in Africa, pp. 67–78, ORSTOM, Paris, 1998, (Backup Publisher: ORSTOM). @incollection{magoulas_genetic_1998, title = {Genetic markers in marine biology and aquaculture research: when to use what}, author = {A Magoulas and G Kotoulas and C Batargias and E Zouros}, editor = {J F Agnese}, year = {1998}, date = {1998-01-01}, booktitle = {Genetics and Aquaculture in Africa}, pages = {67--78}, publisher = {ORSTOM}, address = {Paris}, note = {Backup Publisher: ORSTOM}, keywords = {}, pubstate = {published}, tppubtype = {incollection} } |
Vermeirssen, E L M; Scott, A P; Mylonas, C C; Zohar, Y General and Comparative Endocrinology, 112 (2), pp. 163–177, 1998, ISSN: 00166480, (Publisher: Academic Press Inc.). @article{vermeirssen_gonadotrophin-releasing_1998, title = {Gonadotrophin-releasing hormone agonist stimulates milt fluidity and plasma concentrations of 17,20β-dihydroxylated and 5β-reduced, 3α- hydroxylated C21 steroids in male plaice (Pleuronectes platessa)}, author = {E L M Vermeirssen and A P Scott and C C Mylonas and Y Zohar}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0031791147&doi=10.1006%2fgcen.1998.7132&partnerID=40&md5=084f5de31828689e1bc39bc6cff252d6}, doi = {10.1006/gcen.1998.7132}, issn = {00166480}, year = {1998}, date = {1998-01-01}, journal = {General and Comparative Endocrinology}, volume = {112}, number = {2}, pages = {163--177}, abstract = {Spermiating male plaice were caught in the North Sea and acclimatised to laboratory conditions. In two experiments, males were injected intramuscularly with either microspheres or pellets containing gonadotrophin- releasing hormone agonist (GnRHa). Blood was sampled at 2- to 5-day intervals. Individual blood plasma specimens were assayed for testosterone, 5β-reduced, 3α-hydroxy ('5β,3α') steroids and sulphated 17,20βdihydroxy ('17,20β') steroids. Pooled plasma samples were also assayed for free and sulphated 17,20β-dihydroxy-4-pregnen-3-one, free 11-ketotestosterone, and glucuronidated testosterone and 11-ketotestosterone. Plasma concentrations of all steroids were significantly elevated by GnRHa from 2 to 5 days onwards following treatment. The most marked changes occurred in the concentrations of the sulphated 17,20β steroids, which comprised approximately equal amounts of 5β-pregnane-3α, 17,20β-triol 20-sulphate (3α,17,20β-P-5β-S) and 5β-pregnane-3β,17,20β-triol 20-sulphate, rising from ca. 1 to 30-80 ng/ml in the first and from ca. 8 to 80 ng/ml in the second experiment. Concentrations of 511,3α steroids matched those of 17,20β steroids in one experiment. However, in the other experiment, the two RIAs yielded highly disparate results in about 50% of the fish (including males in the control group). The plasma of these fish contained excessive amounts of 5β,3α- immunoreactive material between 10 and 25 days. This material was identified as 3α,17,21-trihydroxy-5β-pregnan-20-one 21-sulphate (a metabolite of 11- deoxycortisol). All previous studies have indicated that when plasma concentrations of this steroid are high, so are those of 3α,17,20β-P-5β- S. This is the first indication that these steroids are regulated independently. In a third experiment, milt fluidity and production were assessed at 10, 15, and 25 days following GnRHa implantation. Milt volume and fluidity were significantly enhanced by the GnRHa treatment.}, note = {Publisher: Academic Press Inc.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Spermiating male plaice were caught in the North Sea and acclimatised to laboratory conditions. In two experiments, males were injected intramuscularly with either microspheres or pellets containing gonadotrophin- releasing hormone agonist (GnRHa). Blood was sampled at 2- to 5-day intervals. Individual blood plasma specimens were assayed for testosterone, 5β-reduced, 3α-hydroxy ('5β,3α') steroids and sulphated 17,20βdihydroxy ('17,20β') steroids. Pooled plasma samples were also assayed for free and sulphated 17,20β-dihydroxy-4-pregnen-3-one, free 11-ketotestosterone, and glucuronidated testosterone and 11-ketotestosterone. Plasma concentrations of all steroids were significantly elevated by GnRHa from 2 to 5 days onwards following treatment. The most marked changes occurred in the concentrations of the sulphated 17,20β steroids, which comprised approximately equal amounts of 5β-pregnane-3α, 17,20β-triol 20-sulphate (3α,17,20β-P-5β-S) and 5β-pregnane-3β,17,20β-triol 20-sulphate, rising from ca. 1 to 30-80 ng/ml in the first and from ca. 8 to 80 ng/ml in the second experiment. Concentrations of 511,3α steroids matched those of 17,20β steroids in one experiment. However, in the other experiment, the two RIAs yielded highly disparate results in about 50% of the fish (including males in the control group). The plasma of these fish contained excessive amounts of 5β,3α- immunoreactive material between 10 and 25 days. This material was identified as 3α,17,21-trihydroxy-5β-pregnan-20-one 21-sulphate (a metabolite of 11- deoxycortisol). All previous studies have indicated that when plasma concentrations of this steroid are high, so are those of 3α,17,20β-P-5β- S. This is the first indication that these steroids are regulated independently. In a third experiment, milt fluidity and production were assessed at 10, 15, and 25 days following GnRHa implantation. Milt volume and fluidity were significantly enhanced by the GnRHa treatment. |
Mylonas, C C; Zohar, Y; III, Woods L C; Thomas, P; Schulz, R W Hormone profiles of captive striped bass Morone saxatilis during spermiation, and long-term enhancement of milt production Journal Article Journal of the World Aquaculture Society, 29 (4), pp. 379–392, 1998, ISSN: 08938849, (Publisher: World Aquaculture Society). @article{mylonas_hormone_1998, title = {Hormone profiles of captive striped bass Morone saxatilis during spermiation, and long-term enhancement of milt production}, author = {C C Mylonas and Y Zohar and L C Woods III and P Thomas and R W Schulz}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032261401&doi=10.1111%2fj.1749-7345.1998.tb00662.x&partnerID=40&md5=d718fea93ab4b8da4e8fb81c44a88e58}, doi = {10.1111/j.1749-7345.1998.tb00662.x}, issn = {08938849}, year = {1998}, date = {1998-01-01}, journal = {Journal of the World Aquaculture Society}, volume = {29}, number = {4}, pages = {379--392}, abstract = {Plasma profiles of reproductive and thyroid hormones were studied in captive striped bass Morone saxatilis during an 11-wk period encompassing the spawning season, and the effect of a sustained-release gonadotropin-releasing hormone agonist (GnRHa)-delivery system (GnRHa-implant) on milt production was evaluated. The highest percentage of spermiating fish was observed between mid-April and mid-May, and mean total expressible milt ranged from 3.5 to 6.0 mL/kg. Plasma gonadotropin II (GtH II) increased significantly, though inconsistently, during the spermiation period, whereas testosterone and 11-ketotestosterone levels declined continually. Plasma 17,20β-dihydroxy-4-pregnen-3-one and 17,20β,21-dihydroxy-4-pregnen-3-one remained low and unchanged during the peak of the spermiation period, while thyroid hormones were high and fluctuated without exhibiting a trend consistent with spermiation. The observed endocrine profiles suggest that captivity can diminish plasma GtH II and triiodothyronine levels in striped bass. Transfer of spermiating males from large holding tanks to small spawning tanks reduced total expressible milt after 14 d, but treatment with a GnRHa-implant restored milt volume, presumably due to the prolonged elevation of plasma GnRHa and GtH II induced by the GnRHa-implant. Also, treatment with the GnRHa-implant induced a two-to four-fold elevation of expressible milt for at least 20 d compared to control fish, while resulting in only a 5 to 15% decrease in sperm density. It appears that captivity and hatchery operations can diminish milt production in striped bass, and that GnRHa-delivery systems, via sustained elevation of plasma GtH II, can induce long-term enhancement in milt volume without affecting sperm density greatly.}, note = {Publisher: World Aquaculture Society}, keywords = {}, pubstate = {published}, tppubtype = {article} } Plasma profiles of reproductive and thyroid hormones were studied in captive striped bass Morone saxatilis during an 11-wk period encompassing the spawning season, and the effect of a sustained-release gonadotropin-releasing hormone agonist (GnRHa)-delivery system (GnRHa-implant) on milt production was evaluated. The highest percentage of spermiating fish was observed between mid-April and mid-May, and mean total expressible milt ranged from 3.5 to 6.0 mL/kg. Plasma gonadotropin II (GtH II) increased significantly, though inconsistently, during the spermiation period, whereas testosterone and 11-ketotestosterone levels declined continually. Plasma 17,20β-dihydroxy-4-pregnen-3-one and 17,20β,21-dihydroxy-4-pregnen-3-one remained low and unchanged during the peak of the spermiation period, while thyroid hormones were high and fluctuated without exhibiting a trend consistent with spermiation. The observed endocrine profiles suggest that captivity can diminish plasma GtH II and triiodothyronine levels in striped bass. Transfer of spermiating males from large holding tanks to small spawning tanks reduced total expressible milt after 14 d, but treatment with a GnRHa-implant restored milt volume, presumably due to the prolonged elevation of plasma GnRHa and GtH II induced by the GnRHa-implant. Also, treatment with the GnRHa-implant induced a two-to four-fold elevation of expressible milt for at least 20 d compared to control fish, while resulting in only a 5 to 15% decrease in sperm density. It appears that captivity and hatchery operations can diminish milt production in striped bass, and that GnRHa-delivery systems, via sustained elevation of plasma GtH II, can induce long-term enhancement in milt volume without affecting sperm density greatly. |
Kristoffersen, J B; Salvanes, A G V Life history of Maurolicus muelleri in fjordic and oceanic environments Journal Article Journal of Fish Biology, 53 (6), pp. 1324–1341, 1998, ISSN: 00221112. @article{kristoffersen_life_1998, title = {Life history of Maurolicus muelleri in fjordic and oceanic environments}, author = {J B Kristoffersen and A G V Salvanes}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032432626&doi=10.1006%2Fjfbi.1998.0799&partnerID=40&md5=a54c3a94e98cf1400b83d5c425bf3141}, doi = {10.1006/jfbi.1998.0799}, issn = {00221112}, year = {1998}, date = {1998-01-01}, journal = {Journal of Fish Biology}, volume = {53}, number = {6}, pages = {1324--1341}, abstract = {Life history parameters are compared for populations of Maurolicus muelleri from Norwegian fjords and the Norwegian Sea in 1995 and 1996. The mortality rate was higher in the oceanic population (2.55 for males, 2.00 for females) than in the fjords (1.15 for males, 0.97 for females). Fish from the Norwegian Sea population occupied a depth with higher light levels, which may have contributed to the higher mortality through increased visibility to predators. Differences in growth, condition and gonad weight indicated different resource levels caused by different population densities. Recruitment was weak in many fjords in 1994, but not in the Norwegian Sea. It is suggested that wind-generated advection may affect recruitment in fjords on the western coast of Norway.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Life history parameters are compared for populations of Maurolicus muelleri from Norwegian fjords and the Norwegian Sea in 1995 and 1996. The mortality rate was higher in the oceanic population (2.55 for males, 2.00 for females) than in the fjords (1.15 for males, 0.97 for females). Fish from the Norwegian Sea population occupied a depth with higher light levels, which may have contributed to the higher mortality through increased visibility to predators. Differences in growth, condition and gonad weight indicated different resource levels caused by different population densities. Recruitment was weak in many fjords in 1994, but not in the Norwegian Sea. It is suggested that wind-generated advection may affect recruitment in fjords on the western coast of Norway. |
Navajas, M J; Thistlewood, H M A; Lagnel, J; Hughes, C Microsatellite sequences are under-represented in two mite genomes Journal Article Insect Molecular Biology, 7 , pp. 249 – 256, 1998. @article{navajas_microsatellite_1998, title = {Microsatellite sequences are under-represented in two mite genomes}, author = {M J Navajas and H M A Thistlewood and J Lagnel and C Hughes}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0032145763&partnerID=40&md5=0aa4254ea03fcdf7b80276196317feae}, year = {1998}, date = {1998-01-01}, journal = {Insect Molecular Biology}, volume = {7}, pages = {249 -- 256}, abstract = {Microsatellites are known to be a common feature of eukaryote genomes. Here we investigate the presence of microsatellite sequences in the genome of two mite species, Tetranychus urticae and Amblyseius fallacis, based on screening of both mite genomic libraries and Southern blots of these mites that we compare to two vertebrates. No signal with GT15 or a faint smear with CT10 were obtained in Southern analysis for the two mites, whereas both probes strongly bound with vertebrate DNA. Genomic libraries constructed in plasmid and lambda vectors were probed and only two CT microsatellites were isolated for T. urticae. Among eight trinucleotides probes tested, the strongest hybridization signal was detected for T. urticae with CAT and TGA probes. These two classes of repeats were also the most represented in genomic library screenings. However, only sequences with short numbers of units could be detected (< CAT4 or TGA9). Congruency of Southern analysis and screening of partial genomic libraries indicates an under-representation of microsatellite sequences in the two mite genomes. The potential of the scarce repetitive DNA isolated from mites to serve as population genetics markers is discussed on the basis of preliminary assessment of their polymorphism.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Microsatellites are known to be a common feature of eukaryote genomes. Here we investigate the presence of microsatellite sequences in the genome of two mite species, Tetranychus urticae and Amblyseius fallacis, based on screening of both mite genomic libraries and Southern blots of these mites that we compare to two vertebrates. No signal with GT15 or a faint smear with CT10 were obtained in Southern analysis for the two mites, whereas both probes strongly bound with vertebrate DNA. Genomic libraries constructed in plasmid and lambda vectors were probed and only two CT microsatellites were isolated for T. urticae. Among eight trinucleotides probes tested, the strongest hybridization signal was detected for T. urticae with CAT and TGA probes. These two classes of repeats were also the most represented in genomic library screenings. However, only sequences with short numbers of units could be detected (< CAT4 or TGA9). Congruency of Southern analysis and screening of partial genomic libraries indicates an under-representation of microsatellite sequences in the two mite genomes. The potential of the scarce repetitive DNA isolated from mites to serve as population genetics markers is discussed on the basis of preliminary assessment of their polymorphism. |
Goulas, A; Polymenakou, P N; Diallinas, G Molecular determinants involved in function and specificity of nucleobase transporters are conserved in bacteria, fungi, plants and metazoa Inproceedings Proceedings of the 16th SMYTE, Costa-Papiernicka, Slovakia, 1998, (Publication Title: Proceedings of the 16th SMYTE, Small Meeting on Yeast Transport and Energetics Type: Conference Paper). @inproceedings{goulas_molecular_1998, title = {Molecular determinants involved in function and specificity of nucleobase transporters are conserved in bacteria, fungi, plants and metazoa}, author = {A Goulas and P N Polymenakou and G Diallinas}, year = {1998}, date = {1998-01-01}, booktitle = {Proceedings of the 16th SMYTE}, address = {Costa-Papiernicka, Slovakia}, note = {Publication Title: Proceedings of the 16th SMYTE, Small Meeting on Yeast Transport and Energetics Type: Conference Paper}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Hill, J; Hostert, P; Tsiourlis, G; Kasapidis, P; Udelhoven, T; Diemer, C Monitoring 20 years of increased grazing impact on the Greek island of Crete with earth observation satellites Journal Article Journal of Arid Environments, 39 (2), pp. 165–178, 1998, ISSN: 01401963, (Publisher: Academic Press). @article{hill_monitoring_1998, title = {Monitoring 20 years of increased grazing impact on the Greek island of Crete with earth observation satellites}, author = {J Hill and P Hostert and G Tsiourlis and P Kasapidis and T Udelhoven and C Diemer}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032103622&doi=10.1006%2fjare.1998.0392&partnerID=40&md5=5948f809f102880deb29992fcbf6ba03}, doi = {10.1006/jare.1998.0392}, issn = {01401963}, year = {1998}, date = {1998-01-01}, journal = {Journal of Arid Environments}, volume = {39}, number = {2}, pages = {165--178}, abstract = {The degradation of permanent semi-natural vegetation and the resulting acceleration of soil degradation and erosion processes constitute major elements of land degradation in the Mediterranean basin. These elements are triggered by human activities rather than climatic conditions. The Greek island of Crete represents a characteristic case of land degradation resulting from hundreds of years of intensive grazing and fires. Since Greece joined the European Communities in 1981, grazing in mountainous regions has greatly increased due to subsidies that became available through the Common Agricultural Policy. Within a European Research Project on the use of satellite remote sensing for monitoring environmental change in Mediterranean ecosystems (DeMon-2: An Integrated Approach to Assess and Monitor Desertification Processes in the Mediterranean Basin), we have initiated a study to monitor the impact of increased grazing pressure on two mountainous ecosystems in Crete. The approach is based on describing surface conditions and vegetation cover over time with a long-term series of earth observation satellites.}, note = {Publisher: Academic Press}, keywords = {}, pubstate = {published}, tppubtype = {article} } The degradation of permanent semi-natural vegetation and the resulting acceleration of soil degradation and erosion processes constitute major elements of land degradation in the Mediterranean basin. These elements are triggered by human activities rather than climatic conditions. The Greek island of Crete represents a characteristic case of land degradation resulting from hundreds of years of intensive grazing and fires. Since Greece joined the European Communities in 1981, grazing in mountainous regions has greatly increased due to subsidies that became available through the Common Agricultural Policy. Within a European Research Project on the use of satellite remote sensing for monitoring environmental change in Mediterranean ecosystems (DeMon-2: An Integrated Approach to Assess and Monitor Desertification Processes in the Mediterranean Basin), we have initiated a study to monitor the impact of increased grazing pressure on two mountainous ecosystems in Crete. The approach is based on describing surface conditions and vegetation cover over time with a long-term series of earth observation satellites. |
Palma, J; Andrade, J P; Paspatis, M; Divanach, P; Kentouri, M Morphometric characters in gilthead sea bream, Sparus aurata, red porgy, Pagrus pagrus and their hybrids (Sparidae) Journal Article Italian Journal of Zoology, 65 (sup1), pp. 435 – 439, 1998, ISSN: 1125-0003, 1748-5851. @article{palma_morphometric_1998, title = {Morphometric characters in gilthead sea bream, Sparus aurata, red porgy, Pagrus pagrus and their hybrids (Sparidae)}, author = {J Palma and J P Andrade and M Paspatis and P Divanach and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0039702587&partnerID=40&md5=5ee992d3199c1a20e1e909920d20f6d8}, doi = {10.1080/11250009809386861}, issn = {1125-0003, 1748-5851}, year = {1998}, date = {1998-01-01}, journal = {Italian Journal of Zoology}, volume = {65}, number = {sup1}, pages = {435 -- 439}, abstract = {A morphometric comparison was made between Sparus aurata (n = 88), Pagrus pagrus (n = 38) and their hybrid 5. aurata (female) x P. pagrus (male) (n = 81) using 15 morphometric characteristics. The morphometric characteristics of the hybrids were generally intermediate to those of the parental species. However, characteristics related to the body shape (6) were more similar to S. aurata and those characters related to the head shape (9) were more similar to P. pagrus.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A morphometric comparison was made between Sparus aurata (n = 88), Pagrus pagrus (n = 38) and their hybrid 5. aurata (female) x P. pagrus (male) (n = 81) using 15 morphometric characteristics. The morphometric characteristics of the hybrids were generally intermediate to those of the parental species. However, characteristics related to the body shape (6) were more similar to S. aurata and those characters related to the head shape (9) were more similar to P. pagrus. |
Pitta, Paraskevi; Karakassis, Ioannis; Tsapakis, Manolis; Zivanovic, Snezana Natural vs. mariculture induced variability in nutrients and plankton in the eastern Mediterranean Journal Article Hydrobiologia, 391 (1/3), pp. 179–192, 1998, ISSN: 00188158. @article{pitta_natural_1998, title = {Natural vs. mariculture induced variability in nutrients and plankton in the eastern Mediterranean}, author = {Paraskevi Pitta and Ioannis Karakassis and Manolis Tsapakis and Snezana Zivanovic}, url = {http://link.springer.com/10.1023/A:1003501832069}, doi = {10.1023/A:1003501832069}, issn = {00188158}, year = {1998}, date = {1998-01-01}, urldate = {2020-08-18}, journal = {Hydrobiologia}, volume = {391}, number = {1/3}, pages = {179--192}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Dermitzakis, E; Clark, A; Batargias, C; Magoulas, A; Zouros, E Negative covariance indicates mutation bias in a two-locus microsatellite system in the fish Sparus aurata Journal Article Genetics, 150 , pp. 1567–1575, 1998. @article{dermitzakis_negative_1998, title = {Negative covariance indicates mutation bias in a two-locus microsatellite system in the fish Sparus aurata}, author = {E Dermitzakis and A Clark and C Batargias and A Magoulas and E Zouros}, year = {1998}, date = {1998-01-01}, journal = {Genetics}, volume = {150}, pages = {1567--1575}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Kornilios, S; Drakopoulos, P G; Dounas, C Pelagic tar, dissolved/dispersed petroleum hydrocarbons and plastic distribution in the Cretan Sea, Greece Journal Article Marine Pollution Bulletin, 36 (12), pp. 989–993, 1998, ISSN: 0025326X. @article{kornilios_pelagic_1998, title = {Pelagic tar, dissolved/dispersed petroleum hydrocarbons and plastic distribution in the Cretan Sea, Greece}, author = {S Kornilios and P G Drakopoulos and C Dounas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032425472&doi=10.1016%2fS0025-326X%2898%2900102-7&partnerID=40&md5=5c9338c5690910be6dbc8ceebf588557}, doi = {10.1016/S0025-326X(98)00102-7}, issn = {0025326X}, year = {1998}, date = {1998-01-01}, journal = {Marine Pollution Bulletin}, volume = {36}, number = {12}, pages = {989--993}, abstract = {During the first cruise of R/V 'Philia' in July 1997 within the framework of the TALOS programme supported by the creek Ministry of Physical Planning and Public Works, the sampling of floating tar, litter and sea water for dissolved/dispersed petroleum hydrocarbons (DDPH) was carried out in the Cretan Sea. Analysis of these data has allowed a first assessment of the status of floating marine pollution in the region. DDPH measurements showed a mean concentration of 0.145 μg/l of chrysene equivalents (n = 24). Tar and plastics concentrations were in the range of 1 - 4280 and 0 - 1160 μg/m2, respectively. Mean pelagic tar concentration was 318 μg/m2, more than two times higher than what was reported for the area in previous studies. Based on in situ hydrographic observations there is strong evidence that most of the floating tar enters the Cretan Sea through the Ionian Sea.}, keywords = {}, pubstate = {published}, tppubtype = {article} } During the first cruise of R/V 'Philia' in July 1997 within the framework of the TALOS programme supported by the creek Ministry of Physical Planning and Public Works, the sampling of floating tar, litter and sea water for dissolved/dispersed petroleum hydrocarbons (DDPH) was carried out in the Cretan Sea. Analysis of these data has allowed a first assessment of the status of floating marine pollution in the region. DDPH measurements showed a mean concentration of 0.145 μg/l of chrysene equivalents (n = 24). Tar and plastics concentrations were in the range of 1 - 4280 and 0 - 1160 μg/m2, respectively. Mean pelagic tar concentration was 318 μg/m2, more than two times higher than what was reported for the area in previous studies. Based on in situ hydrographic observations there is strong evidence that most of the floating tar enters the Cretan Sea through the Ionian Sea. |
Machias, A; Tsimenides, N; Kokokiris, L; Divanach, P Ring formation on otoliths and scales of Pagrus pagrus: A comparative study Journal Article Journal of Fish Biology, 52 , pp. 350 – 361, 1998. @article{machias_ring_1998, title = {Ring formation on otoliths and scales of Pagrus pagrus: A comparative study}, author = {A Machias and N Tsimenides and L Kokokiris and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0031891494&partnerID=40&md5=87566c6d1571076dbe0eb747e98a83cd}, year = {1998}, date = {1998-01-01}, journal = {Journal of Fish Biology}, volume = {52}, pages = {350 -- 361}, abstract = {One annulus formed on the scales of farmed red porgy Pagrus pagrus each year during March and one opaque zone on the otoliths during June. Scales proved to be more sensitive than otoliths in recording the life history of the red porgy. A false annulus (ring of capture) was observed on the scales of the 0+ age group, but not on the otoliths. A gradual lengthening of the period of annulus formation was observed, from the younger to the older fish. The annulus formation in fish older than 2+ was less synchronized, due to the aquaculture conditions. Scales were more accurate than otoliths in annuli formation. A high percentage of missing or additional opaque zones were observed on otoliths of older fish. These irregularities were related to fish maturation and/or farming conditions. Farmed fish grew three times as fast as the wild fish.}, keywords = {}, pubstate = {published}, tppubtype = {article} } One annulus formed on the scales of farmed red porgy Pagrus pagrus each year during March and one opaque zone on the otoliths during June. Scales proved to be more sensitive than otoliths in recording the life history of the red porgy. A false annulus (ring of capture) was observed on the scales of the 0+ age group, but not on the otoliths. A gradual lengthening of the period of annulus formation was observed, from the younger to the older fish. The annulus formation in fish older than 2+ was less synchronized, due to the aquaculture conditions. Scales were more accurate than otoliths in annuli formation. A high percentage of missing or additional opaque zones were observed on otoliths of older fish. These irregularities were related to fish maturation and/or farming conditions. Farmed fish grew three times as fast as the wild fish. |
Rigos, G; Grigorakis, K; Nengas, I; Christophilogiannis, P; Yiagnisi, M; Koutsodimou, M; Andriopoulou, A; Alexis, M Stress-related pathology seems a significant obstacle for the intensive farming of common dentex, Dentex dentex (linnaeus 1758) Journal Article Bulletin of the European Association of Fish Pathologists, 18 , pp. 15 – 18, 1998. @article{rigos_stress-related_1998, title = {Stress-related pathology seems a significant obstacle for the intensive farming of common dentex, Dentex dentex (linnaeus 1758)}, author = {G Rigos and K Grigorakis and I Nengas and P Christophilogiannis and M Yiagnisi and M Koutsodimou and A Andriopoulou and M Alexis}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0003198749&partnerID=40&md5=304dccbac8bdb17b0c47eb5a179a0be7}, year = {1998}, date = {1998-01-01}, journal = {Bulletin of the European Association of Fish Pathologists}, volume = {18}, pages = {15 -- 18}, abstract = {High mortalities of pathological origin were evident when juvenile common dentex (Dentex dentex) L. were exposed to stress situations and increased handling. The pathological examination of an outbreak revealed the presence of two parasites, two bacterial pathogens and a chlamydia-like organism. These pathological findings that were accompanied with a moderate agonistic behaviour of the fish and cannibalism signs seem to be associated with the relatively high susceptibility of common dentex to stress conditions. The stressed individuals found to succumb easily to disease problems leading to progressive mortalities. Similar findings were appeared in the investigation of a new disease incidence due to bacterial infections. Neither pathological signs nor agonistic behaviour or cannibalism bites were evident when the fish were exposed to minimum stress situations and not extensively handled.}, keywords = {}, pubstate = {published}, tppubtype = {article} } High mortalities of pathological origin were evident when juvenile common dentex (Dentex dentex) L. were exposed to stress situations and increased handling. The pathological examination of an outbreak revealed the presence of two parasites, two bacterial pathogens and a chlamydia-like organism. These pathological findings that were accompanied with a moderate agonistic behaviour of the fish and cannibalism signs seem to be associated with the relatively high susceptibility of common dentex to stress conditions. The stressed individuals found to succumb easily to disease problems leading to progressive mortalities. Similar findings were appeared in the investigation of a new disease incidence due to bacterial infections. Neither pathological signs nor agonistic behaviour or cannibalism bites were evident when the fish were exposed to minimum stress situations and not extensively handled. |
Koukouras, A; Kühlmann, D; Voultsiadou, E; Vafidis, D; Dounas, C; Chintiroglou, C; Koutsoubas, D The macrofaunal assemblage associated with the scleractinian coral Cladocora caespitosa (L.) in the Aegean Sea Journal Article Annales de l'Institute Oceanographique, 74 (2), pp. 97–114, 1998, ISSN: 00789682. @article{koukouras_macrofaunal_1998, title = {The macrofaunal assemblage associated with the scleractinian coral Cladocora caespitosa (L.) in the Aegean Sea}, author = {A Koukouras and D Kühlmann and E Voultsiadou and D Vafidis and C Dounas and C Chintiroglou and D Koutsoubas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032226464&partnerID=40&md5=a085efb4c6388beeabc74c3201106b21}, issn = {00789682}, year = {1998}, date = {1998-01-01}, journal = {Annales de l'Institute Oceanographique}, volume = {74}, number = {2}, pages = {97--114}, abstract = {Colonies of the Scleractinian C. caespitosa were collected from two different sites in the Chalkidiki Peninsula (North Aegean Sea. Greece) in September 1987. The information obtained from multidimensional scaling and analysis of the data revealed a difference in the qualitative and quantitative composition of the associated assemblage between the two sites. Two distinguishable benthic communities exist, due to the different prevailing physicochemical parameters. At the shallow site, crustaceans and molluscs are the dominant taxa, in contrast to polychaetes at the deepest site. At both sites, deposit feeders dominate, with their numbers increasing with depth. In contrast, numbers of suspension feeders decrease with depth.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Colonies of the Scleractinian C. caespitosa were collected from two different sites in the Chalkidiki Peninsula (North Aegean Sea. Greece) in September 1987. The information obtained from multidimensional scaling and analysis of the data revealed a difference in the qualitative and quantitative composition of the associated assemblage between the two sites. Two distinguishable benthic communities exist, due to the different prevailing physicochemical parameters. At the shallow site, crustaceans and molluscs are the dominant taxa, in contrast to polychaetes at the deepest site. At both sites, deposit feeders dominate, with their numbers increasing with depth. In contrast, numbers of suspension feeders decrease with depth. |
Magoulas, A; Gjetvaj, B; Terzoglou, V; Zouros, E Three polymorphic microsatellites in the Japanese oyster, Crassostrea gigas (Thunberg) Journal Article Animal Genetics, 29 , pp. 69 – 70, 1998. @article{magoulas_three_1998, title = {Three polymorphic microsatellites in the Japanese oyster, Crassostrea gigas (Thunberg)}, author = {A Magoulas and B Gjetvaj and V Terzoglou and E Zouros}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0031869608&partnerID=40&md5=d61c90542e845be7feace22b67b93902}, year = {1998}, date = {1998-01-01}, journal = {Animal Genetics}, volume = {29}, pages = {69 -- 70}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
1997 |
Koumoundouros, G; Oran, G; Divanach, P; Stefanakis, S; Kentouri, M The opercular complex deformity in intensive gilthead sea bream (Sparus aurata L.) larviculture. Moment of apparition and description Journal Article Aquaculture, 156 (1-2), pp. 165–177, 1997, ISSN: 00448486. @article{koumoundouros_opercular_1997, title = {The opercular complex deformity in intensive gilthead sea bream (Sparus aurata L.) larviculture. Moment of apparition and description}, author = {G Koumoundouros and G Oran and P Divanach and S Stefanakis and M Kentouri}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848697892940}, doi = {10.1016/S0044-8486(97)89294-0}, issn = {00448486}, year = {1997}, date = {1997-10-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {156}, number = {1-2}, pages = {165--177}, abstract = {External morphology, head morphometry and osteology were used for early detection, description and heuristic study of the opercular complex abnormalities of intensively reared gilthead sea bream larvae (Sparus aurata). The first evidence of malformations were observed in larvae of 6.1 mm total length (TL). The abnormalities were fully developed during metamorphosis (11.0-22.0 mm TL). i.e., before the end of the hatchery phase. In 81.4% of the abnormal fish, the problem was unilateral with similar right/left frequency. In 85.5% of the cases, it was associated with severe foldings and twists of the operculum and/or suboperculum and rarely with an atrophy of these bones. The etiology, aquaculture applications and research targets for the erasure of skeletal malformations are discussed.}, keywords = {}, pubstate = {published}, tppubtype = {article} } External morphology, head morphometry and osteology were used for early detection, description and heuristic study of the opercular complex abnormalities of intensively reared gilthead sea bream larvae (Sparus aurata). The first evidence of malformations were observed in larvae of 6.1 mm total length (TL). The abnormalities were fully developed during metamorphosis (11.0-22.0 mm TL). i.e., before the end of the hatchery phase. In 81.4% of the abnormal fish, the problem was unilateral with similar right/left frequency. In 85.5% of the cases, it was associated with severe foldings and twists of the operculum and/or suboperculum and rarely with an atrophy of these bones. The etiology, aquaculture applications and research targets for the erasure of skeletal malformations are discussed. |
Rueda, F M; Lopez, J A; Martinez, F J; Zamora, S; Divanach, P; Kentouri, M Fatty acids in muscle of wild and farmed red porgy, Pagrus pagrus Journal Article Aquaculture Nutrition, 3 (3), pp. 161–165, 1997, ISSN: 1353-5773, 1365-2095. @article{rueda_fatty_1997, title = {Fatty acids in muscle of wild and farmed red porgy, Pagrus pagrus}, author = {F M Rueda and J A Lopez and F J Martinez and S Zamora and P Divanach and M Kentouri}, url = {http://doi.wiley.com/10.1046/j.1365-2095.1997.00088.x}, doi = {10.1046/j.1365-2095.1997.00088.x}, issn = {1353-5773, 1365-2095}, year = {1997}, date = {1997-09-01}, urldate = {2020-08-18}, journal = {Aquaculture Nutrition}, volume = {3}, number = {3}, pages = {161--165}, abstract = {The total fat content and the fatty acids of total lipids (TL), phospholipids (PL) and triacylglycerols (TAG) were analysed in white muscle from wild and reared red porgy, Pagrus pagrus (L.). The fat percentage was higher in reared (3.03 ± 0.57%) (mean ± SE) than wild (0.65 ± 0.03%) fish. The fatty acid pattern of reared porgy TL reflected the diet’s lipid. Wild red porgy showed higher levels of ω3 and ω6 polyunsaturated fatty acids (PUFA) than reared, but both the atherogenic index and the index of thrombogenicity were very low for both wild and reared red porgy. The lipid fractions showed that the PL were composed of very high levels of PUFA, especially 22:6ω3, and their composition seemed to be better regulated than the composition of TAG. TAG showed greater differences between wild and reared fish, presumably because they reflected the dietary content of lipids. PUFA were the principal component of wild red porgy TAG.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The total fat content and the fatty acids of total lipids (TL), phospholipids (PL) and triacylglycerols (TAG) were analysed in white muscle from wild and reared red porgy, Pagrus pagrus (L.). The fat percentage was higher in reared (3.03 ± 0.57%) (mean ± SE) than wild (0.65 ± 0.03%) fish. The fatty acid pattern of reared porgy TL reflected the diet’s lipid. Wild red porgy showed higher levels of ω3 and ω6 polyunsaturated fatty acids (PUFA) than reared, but both the atherogenic index and the index of thrombogenicity were very low for both wild and reared red porgy. The lipid fractions showed that the PL were composed of very high levels of PUFA, especially 22:6ω3, and their composition seemed to be better regulated than the composition of TAG. TAG showed greater differences between wild and reared fish, presumably because they reflected the dietary content of lipids. PUFA were the principal component of wild red porgy TAG. |
Øie, Gunvor; Makridis, Pavlos; Reitan, Kjell Inge; Olsen, Yngvar Protein and carbon utilization of rotifers (Brachionus plicatilis) in first feeding of turbot larvae (Scophthalmus maximus L.) Journal Article Aquaculture, 153 (1-2), pp. 103–122, 1997, ISSN: 00448486. @article{oie_protein_1997, title = {Protein and carbon utilization of rotifers (Brachionus plicatilis) in first feeding of turbot larvae (Scophthalmus maximus L.)}, author = {Gunvor Øie and Pavlos Makridis and Kjell Inge Reitan and Yngvar Olsen}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848696015141}, doi = {10.1016/S0044-8486(96)01514-1}, issn = {00448486}, year = {1997}, date = {1997-06-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {153}, number = {1-2}, pages = {103--122}, abstract = {The effect of three different rotifer enrichments was examined on growth, survival, pigmentation and viability of first feeding turbot larvae. The diets differed in rotifer content of protein, lipid and ratio of protein/lipid. The diets were fed to turbot with or without algae (Isochrysis galbana) added to the larval tanks. The turbot larvae were fed rotifers for 10 days and thereafter the same Artemia diet was fed to all treatments for the rest of the experimental period. Growth and survival of fish larvae were higher in tanks containing algae than in tanks where no algae were added. Independent of algal addition, the highest growth rate and survival was obtained by feeding rotifers containing the highest protein content. Larvae reared in greenwater consumed higher numbers of rotifers during the stagnant period than larvae kept in clearwater conditions, while analysis of the larval gut contents showed lower rotifer numbers in the gut of larvae reared in greenwater conditions. This must imply longer residence time of the food in the larval gut, and presumably also higher digestion and assimilation efficiencies of larvae maintained without algae than in larvae maintained with algae. Calculation of protein and carbon conversion efficiency showed higher utilization in larvae maintained without algae (18-28% for protein, 12-19% for carbon) than in larvae maintained with algae (6-9% for protein, 4-7% for carbon). No significant differences in pigmentation rata and stress sensitivity were observed among the larvae of the various treatments.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The effect of three different rotifer enrichments was examined on growth, survival, pigmentation and viability of first feeding turbot larvae. The diets differed in rotifer content of protein, lipid and ratio of protein/lipid. The diets were fed to turbot with or without algae (Isochrysis galbana) added to the larval tanks. The turbot larvae were fed rotifers for 10 days and thereafter the same Artemia diet was fed to all treatments for the rest of the experimental period. Growth and survival of fish larvae were higher in tanks containing algae than in tanks where no algae were added. Independent of algal addition, the highest growth rate and survival was obtained by feeding rotifers containing the highest protein content. Larvae reared in greenwater consumed higher numbers of rotifers during the stagnant period than larvae kept in clearwater conditions, while analysis of the larval gut contents showed lower rotifer numbers in the gut of larvae reared in greenwater conditions. This must imply longer residence time of the food in the larval gut, and presumably also higher digestion and assimilation efficiencies of larvae maintained without algae than in larvae maintained with algae. Calculation of protein and carbon conversion efficiency showed higher utilization in larvae maintained without algae (18-28% for protein, 12-19% for carbon) than in larvae maintained with algae (6-9% for protein, 4-7% for carbon). No significant differences in pigmentation rata and stress sensitivity were observed among the larvae of the various treatments. |
Koumoundouros, G; Gagliardi, F; Divanach, P; Boglione, C; Cataudella, S; Kentouri, M Normal and abnormal osteological development of caudal fin in Sparus aurata L. fry Journal Article Aquaculture, 149 (3-4), pp. 215–226, 1997, ISSN: 00448486. @article{koumoundouros_normal_1997, title = {Normal and abnormal osteological development of caudal fin in Sparus aurata L. fry}, author = {G Koumoundouros and F Gagliardi and P Divanach and C Boglione and S Cataudella and M Kentouri}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848696014433}, doi = {10.1016/S0044-8486(96)01443-3}, issn = {00448486}, year = {1997}, date = {1997-03-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {149}, number = {3-4}, pages = {215--226}, abstract = {Normal osteological development of the caudal fin in gilthead sea bream is described at the larval and juvenile stages. It was used as a standard for the study of a sea bream larval population with characteristic osteological deviations which give rise to abnormal juveniles. Normal specimens were reared under extensive rearing conditions, and abnormal specimens under intensive rearing conditions. The first hypural elements appearing in the abnormal preflexion larvae were deformed and displaced. With development, all elements related to the caudal region (parhypural, hypuralia, epuralia, vertebra centra, neural and haemal processes) formed abnormally. The abnormalities were extra-numerous elements, fusions, deformities and displacements of the elements.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Normal osteological development of the caudal fin in gilthead sea bream is described at the larval and juvenile stages. It was used as a standard for the study of a sea bream larval population with characteristic osteological deviations which give rise to abnormal juveniles. Normal specimens were reared under extensive rearing conditions, and abnormal specimens under intensive rearing conditions. The first hypural elements appearing in the abnormal preflexion larvae were deformed and displaced. With development, all elements related to the caudal region (parhypural, hypuralia, epuralia, vertebra centra, neural and haemal processes) formed abnormally. The abnormalities were extra-numerous elements, fusions, deformities and displacements of the elements. |
Pitta, P; Tsapakis, E; Dafnomili, E; Karakassis, Ioannis Aquaculture impact on the planktonic system in three fish farms Inproceedings pp. 219–222, 1997, (Publication Title: 5th Pan-Hellenic Symposium of Oceanography and Fisheries). @inproceedings{pitta_aquaculture_1997, title = {Aquaculture impact on the planktonic system in three fish farms}, author = {P Pitta and E Tsapakis and E Dafnomili and Ioannis Karakassis}, year = {1997}, date = {1997-01-01}, volume = {II}, pages = {219--222}, note = {Publication Title: 5th Pan-Hellenic Symposium of Oceanography and Fisheries}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Rigos, G; Grigorakis, K; Christophilogannis, P; Nengas, I; Alexis, M Ceratomyxa spp. (myxosporea) infection in cultured common dentex from Greece Journal Article Bulletin of the European Association of Fish Pathologists, 17 , pp. 174 – 176, 1997. @article{rigos_ceratomyxa_1997, title = {Ceratomyxa spp. (myxosporea) infection in cultured common dentex from Greece}, author = {G Rigos and K Grigorakis and P Christophilogannis and I Nengas and M Alexis}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0001359151&partnerID=40&md5=c9888b53eed53faf487895ab5061da21}, year = {1997}, date = {1997-01-01}, journal = {Bulletin of the European Association of Fish Pathologists}, volume = {17}, pages = {174 -- 176}, abstract = {Ceratomyxosis was found in common dentex (Dentex dentex L.), a new cultured Sparidae species in Mediterranean mariculture. The cause of the disease appeared to be the myxosporean Ceratomyxa diplodae and C. labrakis with the first being more prevalent. Both coelozoic species were recovered from the bile and the intestinal mucosa without affecting other tissues, however, a possible disorder of the digestive functions may be the cause of the moderate emaciation and mortality of the fish.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Ceratomyxosis was found in common dentex (Dentex dentex L.), a new cultured Sparidae species in Mediterranean mariculture. The cause of the disease appeared to be the myxosporean Ceratomyxa diplodae and C. labrakis with the first being more prevalent. Both coelozoic species were recovered from the bile and the intestinal mucosa without affecting other tissues, however, a possible disorder of the digestive functions may be the cause of the moderate emaciation and mortality of the fish. |
Mylonas, C C; Scott, A P; Vermeirssen, E L M; Zohar, Y Biology of Reproduction, 57 (3), pp. 669–675, 1997, ISSN: 00063363. @article{mylonas_changes_1997, title = {Changes in plasma gonadotropin II and sex steroid hormones, and sperm production of striped bass after treatment with controlled-release gonadotropin-releasing hormone agonist-delivery systems}, author = {C C Mylonas and A P Scott and E L M Vermeirssen and Y Zohar}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030838590&doi=10.1095%2fbiolreprod57.3.669&partnerID=40&md5=611a3480a18c07ff0a4dcaa0996716a2}, doi = {10.1095/biolreprod57.3.669}, issn = {00063363}, year = {1997}, date = {1997-01-01}, journal = {Biology of Reproduction}, volume = {57}, number = {3}, pages = {669--675}, abstract = {Spermiating striped bass, a perciform fish, were treated with two controlled-release gonadotropin-releasing hormone agonist (GnRHa)-delivery systems, and the resulting changes in plasma gonadotropin II (GTH II), testosterone (T), 11-ketotestosterone (11-KT), 17,20β-dihydroxy-4-pregnen- 3-one (17,20β-P), and 17,20β,21-trihydroxy-4-pregnen-3-one (17,20β,21-P) were correlated with changes in milt production and sperm density. GnRHa- delivery systems induced a sustained elevation of plasma GnRHa and GTH II for 14 days. Plasma T levels were unchanged after GnRHa treatment, while 11-KT levels increased significantly. Plasma 17,20β-P also increased after GnRHa treatment and remained elevated compared to levels in controls, while plasma 17,20β,21-P levels were unaffected. Both GnRHa-delivery systems induced many-fold increases in total expressible milt, lasting throughout the 14 days of the study. Sperm density decreased 2 days after GnRHa treatment, with a subsequent increase by Day 7. This study demonstrates that GnRHa-delivery systems induce a sustained elevation of plasma GTH II levels in striped bass, resulting in a long-term enhancement of milt production. The endocrine changes observed suggest that 11-KT and 17,20β-P, but not 17,20β,21-P, are mediating the effects of GTH II on spermiation.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Spermiating striped bass, a perciform fish, were treated with two controlled-release gonadotropin-releasing hormone agonist (GnRHa)-delivery systems, and the resulting changes in plasma gonadotropin II (GTH II), testosterone (T), 11-ketotestosterone (11-KT), 17,20β-dihydroxy-4-pregnen- 3-one (17,20β-P), and 17,20β,21-trihydroxy-4-pregnen-3-one (17,20β,21-P) were correlated with changes in milt production and sperm density. GnRHa- delivery systems induced a sustained elevation of plasma GnRHa and GTH II for 14 days. Plasma T levels were unchanged after GnRHa treatment, while 11-KT levels increased significantly. Plasma 17,20β-P also increased after GnRHa treatment and remained elevated compared to levels in controls, while plasma 17,20β,21-P levels were unaffected. Both GnRHa-delivery systems induced many-fold increases in total expressible milt, lasting throughout the 14 days of the study. Sperm density decreased 2 days after GnRHa treatment, with a subsequent increase by Day 7. This study demonstrates that GnRHa-delivery systems induce a sustained elevation of plasma GTH II levels in striped bass, resulting in a long-term enhancement of milt production. The endocrine changes observed suggest that 11-KT and 17,20β-P, but not 17,20β,21-P, are mediating the effects of GTH II on spermiation. |
Dounas, C; Kallianiotis, Argyris Construction of an artificial reef park and creation of a marine protected zone in order to prevent fishing activities off the coasts of Thrace (N. Aegean Sea) Journal Article Fishing News, 197 , pp. 50–55, 1997, ((in Greek)). @article{dounas_construction_1997, title = {Construction of an artificial reef park and creation of a marine protected zone in order to prevent fishing activities off the coasts of Thrace (N. Aegean Sea)}, author = {C Dounas and Argyris Kallianiotis}, year = {1997}, date = {1997-01-01}, journal = {Fishing News}, volume = {197}, pages = {50--55}, note = {(in Greek)}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Mylonas, C C; III, Woods L C; Zohar, Y Cyto-histological examination of post-vitellogenesis and final oocyte maturation in captive-reared striped bass Journal Article Journal of Fish Biology, 50 (1), pp. 34–49, 1997, ISSN: 00221112. @article{mylonas_cyto-histological_1997, title = {Cyto-histological examination of post-vitellogenesis and final oocyte maturation in captive-reared striped bass}, author = {C C Mylonas and L C Woods III and Y Zohar}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030910478&doi=10.1006%2fjfbi.1996.0275&partnerID=40&md5=abcc991c7bc67da2d0568b13c38816b3}, doi = {10.1006/jfbi.1996.0275}, issn = {00221112}, year = {1997}, date = {1997-01-01}, journal = {Journal of Fish Biology}, volume = {50}, number = {1}, pages = {34--49}, abstract = {The ovarian development of captive-reared, striped bass Morone saxatilis was examined during a 10-week period encompassing the spawning season. Vitellogenic oocytes in March had a mean diameter of 838 ± 18 μm and did not grow significantly therafter. Except from one non-hormone-treated fish, all females failed to undergo final oocyte maturation (FOM) and their ovaries became atretic with the onset of high spring temperatures. A clearing fixative was found useful in identifying early of atresia, evident by the absence of the germinal vesicle (GV). Final oocyte maturation of fish maturation of fish treated with gonadotropin-releasing hormone agonist (GnRhA) consisted of two phases. Early FOM lasted from 1 to 3 weeks, and was associated with lipid-droplet coalescence, and displacement of the GV and yolk globules to the peripheral cytoplasm. Late FOM lasted textless24 h, and consisted of yolk-globule coalescence and GV breakdown (GVBD). Ovulated eggs had completely coalesced lipid and yolk masses, with cortical alveoli lined against the cell wall. Both phases of FOM were associated with significant increases in oocyte diameter. Striped boss oocytes showed important morphological differences compared to oocytes of other members of the Moronidae family, in terms of percentage lipid content, chorion thickness and degree of hydration after ovulation.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The ovarian development of captive-reared, striped bass Morone saxatilis was examined during a 10-week period encompassing the spawning season. Vitellogenic oocytes in March had a mean diameter of 838 ± 18 μm and did not grow significantly therafter. Except from one non-hormone-treated fish, all females failed to undergo final oocyte maturation (FOM) and their ovaries became atretic with the onset of high spring temperatures. A clearing fixative was found useful in identifying early of atresia, evident by the absence of the germinal vesicle (GV). Final oocyte maturation of fish maturation of fish treated with gonadotropin-releasing hormone agonist (GnRhA) consisted of two phases. Early FOM lasted from 1 to 3 weeks, and was associated with lipid-droplet coalescence, and displacement of the GV and yolk globules to the peripheral cytoplasm. Late FOM lasted textless24 h, and consisted of yolk-globule coalescence and GV breakdown (GVBD). Ovulated eggs had completely coalesced lipid and yolk masses, with cortical alveoli lined against the cell wall. Both phases of FOM were associated with significant increases in oocyte diameter. Striped boss oocytes showed important morphological differences compared to oocytes of other members of the Moronidae family, in terms of percentage lipid content, chorion thickness and degree of hydration after ovulation. |
Pavlidis, M; Berry, M; Divanach, P; Kentouri, M Diel pattern of haematocrit, serum metabolites, osmotic pressure, electrolytes and thyroid hormones in sea bass and sea bream Journal Article Aquaculture International, 5 , pp. 237 – 247, 1997. @article{pavlidis_diel_1997, title = {Diel pattern of haematocrit, serum metabolites, osmotic pressure, electrolytes and thyroid hormones in sea bass and sea bream}, author = {M Pavlidis and M Berry and P Divanach and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0000527603&partnerID=40&md5=43ffd0cedb64e4f7359a2e7f9a22c7ef}, year = {1997}, date = {1997-01-01}, journal = {Aquaculture International}, volume = {5}, pages = {237 -- 247}, abstract = {The diel pattern of haematocrit, serum metabolites, electrolytes and thyroid hormones are described in sea bass, Dicentrarchus labrax, and sea bream, Sparus auratus, held under 12L:12D light cycle (0600-1800 h light). No diel rhythmicity in haematocrit, total proteins, potassium and chloride was observed. Maximum values of glucose, osmotic pressure and sodium were recorded at dusk (1800 h) in both species, irrespective of differences in the size of fish and feeding regime used. Maximum and minimum triiodothyronine values were found during the scotophase in bass and during the photophase in bream. Maximum thyroxine levels occurred at 1400 h (bass) and 1800 h (bream) and minimum during the scotophase, in both species. The light/dark alternation seems to be an important synchronizer of the observed diel changes. Results are discussed in relation to the timing of feeding and the general activity of the fish.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The diel pattern of haematocrit, serum metabolites, electrolytes and thyroid hormones are described in sea bass, Dicentrarchus labrax, and sea bream, Sparus auratus, held under 12L:12D light cycle (0600-1800 h light). No diel rhythmicity in haematocrit, total proteins, potassium and chloride was observed. Maximum values of glucose, osmotic pressure and sodium were recorded at dusk (1800 h) in both species, irrespective of differences in the size of fish and feeding regime used. Maximum and minimum triiodothyronine values were found during the scotophase in bass and during the photophase in bream. Maximum thyroxine levels occurred at 1400 h (bass) and 1800 h (bream) and minimum during the scotophase, in both species. The light/dark alternation seems to be an important synchronizer of the observed diel changes. Results are discussed in relation to the timing of feeding and the general activity of the fish. |
Tselepides, A; Polychronaki, T; Dafnomili, E; Plaiti, W; Zivanovic, S Distribution of nutrients, chloroplastic pigments, POC, PON and ATP in the Cretan Sea (NE Mediterranean): Seasonal and interannual variability Inproceedings pp. 189–193, 1997, (Publication Title: The 5th Pan-Hellenic Symposium of Oceanography and Fisheries). @inproceedings{tselepides_distribution_1997, title = {Distribution of nutrients, chloroplastic pigments, POC, PON and ATP in the Cretan Sea (NE Mediterranean): Seasonal and interannual variability}, author = {A Tselepides and T Polychronaki and E Dafnomili and W Plaiti and S Zivanovic}, year = {1997}, date = {1997-01-01}, volume = {I}, pages = {189--193}, note = {Publication Title: The 5th Pan-Hellenic Symposium of Oceanography and Fisheries}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Koutsoubas, D; Dounas, C; Petihakis, G; Arvanitidis, C; Triantafyllou, G; Dafnomili, E; Zivanovic, S Dynamics of the physicochemical parameters in water column and in the sediments of the Gialova lagoon (NW Peloponnese, Ionian Sea) Inproceedings pp. 351–353, Kavala, Greece, 1997, (Publication Title: Seventh Symposium on Oceanography and Fisheries, Κavala). @inproceedings{koutsoubas_dynamics_1997, title = {Dynamics of the physicochemical parameters in water column and in the sediments of the Gialova lagoon (NW Peloponnese, Ionian Sea)}, author = {D Koutsoubas and C Dounas and G Petihakis and C Arvanitidis and G Triantafyllou and E Dafnomili and S Zivanovic}, year = {1997}, date = {1997-01-01}, volume = {II}, pages = {351--353}, address = {Kavala, Greece}, note = {Publication Title: Seventh Symposium on Oceanography and Fisheries, Κavala}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Chatzifotis, S; Takeuchi, T Aquaculture, 158 (1-2), pp. 129–140, 1997, ISSN: 00448486. @article{chatzifotis_effect_1997-1, title = {Effect of supplemental camitine on body weight loss, proximate and lipid compositions and carnitine content of red sea bream (Pagrus major) during starvation}, author = {S Chatzifotis and T Takeuchi}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0031457222&doi=10.1016%2fS0044-8486%2897%2900175-0&partnerID=40&md5=2050750033b443a4d28cf92926fcb924}, doi = {10.1016/S0044-8486(97)00175-0}, issn = {00448486}, year = {1997}, date = {1997-01-01}, journal = {Aquaculture}, volume = {158}, number = {1-2}, pages = {129--140}, abstract = {The effect of supplemental dietary carnitine on the body weight loss, lipid composition and carnitine content of dorsal muscle of red sea bream during starvation was investigated. Two experimental groups of red sea bream fingerlings were used. One group was fed a control diet (Diet 1; textless 0.09 g carnitine/kg feed) for 30 days prior to starvation while the second group was fed a carnitine-supplemented diet (Diet 2; 4 g carnitine/kg feed). Then fish were deprived of feed for 30 days. During the feeding period, fish fed Diet 2 accumulated free-carnitine in their dorsal muscle. The acid-soluble acylcarnitine content remained constant, indicating a redistribution of free and esterified carnitine. Although the long-chain acylcarnitine in the dorsal muscle increased during starvagion, no difference was observed in the long-chain acylcarnitine content between the carnitine and the control group. The higher concentration of carnitine in the diet prior to starvation clearly did not show any effect on body weight loss or on the proximate and fatty acid compositions of dorsal muscle nor on the fatty acid composition of the liver. Carnitine supplementation prior to starvation did not show any clear effect on lipid classes in dorsal muscle and liver or on crude lipid and water concentration in liver during starvation.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The effect of supplemental dietary carnitine on the body weight loss, lipid composition and carnitine content of dorsal muscle of red sea bream during starvation was investigated. Two experimental groups of red sea bream fingerlings were used. One group was fed a control diet (Diet 1; textless 0.09 g carnitine/kg feed) for 30 days prior to starvation while the second group was fed a carnitine-supplemented diet (Diet 2; 4 g carnitine/kg feed). Then fish were deprived of feed for 30 days. During the feeding period, fish fed Diet 2 accumulated free-carnitine in their dorsal muscle. The acid-soluble acylcarnitine content remained constant, indicating a redistribution of free and esterified carnitine. Although the long-chain acylcarnitine in the dorsal muscle increased during starvagion, no difference was observed in the long-chain acylcarnitine content between the carnitine and the control group. The higher concentration of carnitine in the diet prior to starvation clearly did not show any effect on body weight loss or on the proximate and fatty acid compositions of dorsal muscle nor on the fatty acid composition of the liver. Carnitine supplementation prior to starvation did not show any clear effect on lipid classes in dorsal muscle and liver or on crude lipid and water concentration in liver during starvation. |
Divanach, P; Papandroulakis, N; Anastasiadis, P; Koumoundouros, G; Kentouri, M Aquaculture, 156 , pp. 145 – 155, 1997. @article{divanach_effect_1997, title = {Effect of water currents on the development of skeletal deformities in sea bass (Dicentrarchus labtax L.) with functional swimbladder during postlarval and nursery phase}, author = {P Divanach and N Papandroulakis and P Anastasiadis and G Koumoundouros and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0030678081&partnerID=40&md5=ff2eb4edf54484b5987625d1168e5cac}, year = {1997}, date = {1997-01-01}, journal = {Aquaculture}, volume = {156}, pages = {145 -- 155}, abstract = {Lordosis in reared sea bass was previously related either to the absence of swimbladder, or to bad or late inflation of this organ when damages were irreversible. Our results prove that these explanations are insufficient and that similar deformations may occur frequently in fry with functional swimbladder during the early weaning phase, if currents in tanks are higher than 10 cm s-1.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Lordosis in reared sea bass was previously related either to the absence of swimbladder, or to bad or late inflation of this organ when damages were irreversible. Our results prove that these explanations are insufficient and that similar deformations may occur frequently in fry with functional swimbladder during the early weaning phase, if currents in tanks are higher than 10 cm s-1. |
Tsapakis, E; Karakassis, Ioannis; Xatziyanni, E; Maidanou, M Effects of aquaculture in three coastal areas Journal Article Fisheries News, 199 , pp. 90–95, 1997. @article{tsapakis_effects_1997, title = {Effects of aquaculture in three coastal areas}, author = {E Tsapakis and Ioannis Karakassis and E Xatziyanni and M Maidanou}, year = {1997}, date = {1997-01-01}, journal = {Fisheries News}, volume = {199}, pages = {90--95}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Larsson, D G J; Mylonas, C C; Zohar, Y; Crim, L W Canadian Journal of Fisheries and Aquatic Sciences, 54 (9), pp. 1957–1964, 1997, ISSN: 0706652X. @article{larsson_gonadotropin-releasing_1997, title = {Gonadotropin-releasing hormone analogue (GnRH-A) induces multiple ovulations of high-quality eggs in a cold-water, batch-spawning teleost, the yellowtail flounder (Pleuronectes ferrugineus)}, author = {D G J Larsson and C C Mylonas and Y Zohar and L W Crim}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0031449685&doi=10.1139%2fcjfas-54-9-1957&partnerID=40&md5=52dbd73406a33867ec5f010c83f98ca6}, doi = {10.1139/cjfas-54-9-1957}, issn = {0706652X}, year = {1997}, date = {1997-01-01}, journal = {Canadian Journal of Fisheries and Aquatic Sciences}, volume = {54}, number = {9}, pages = {1957--1964}, abstract = {Sustained delivery systems for a gonadotropin-releasing hormone analogue (GnRH-A) were used for the first time in a cold-ocean teleost for inducing multiple ovulations. Intramuscular implants of 75-224 μg/kg GnRH-A ((D-Ala6,Pro9-NHEt)LHRH) were administered to female yellowtail flounder (Pleuronectes ferrugineus) either as a cholesterol pellet or as biodegradable microspheres. Females implanted with GnRH-A ovulated 3-4 weeks earlier than controls, producing multiple batches of high-quality eggs (average rates of fertilization 66-68% and hatching 55-60%); control fish eggs had significantly lower fertilization (39%) and hatching rates (25%). GnRH-A treatment doubled the egg production, synchronized the females, and shortened the interovulatory periods. The proportion of round, clear, and floating eggs lacking a perivitelline space before fertilization was an indicator of the fertilization (r2= 0.60) and hatching (r2 = 0.56) success. Moreover, egg collections of less than 10 mL varied greatly in quality, whereas larger batches, generally promoted by GnRH-A, consisted of relatively high-quality eggs. GnRH-A treated fish had increased plasma levels of estradiol- 17β 4 days after implantation. A model for prediction of the time between hormone treatment and first spawning (T; days) was developed using the initial follicle diameter (D; μm) as the only predictor (T = 365(D - 380)-082; r2 = 0.91).}, keywords = {}, pubstate = {published}, tppubtype = {article} } Sustained delivery systems for a gonadotropin-releasing hormone analogue (GnRH-A) were used for the first time in a cold-ocean teleost for inducing multiple ovulations. Intramuscular implants of 75-224 μg/kg GnRH-A ((D-Ala6,Pro9-NHEt)LHRH) were administered to female yellowtail flounder (Pleuronectes ferrugineus) either as a cholesterol pellet or as biodegradable microspheres. Females implanted with GnRH-A ovulated 3-4 weeks earlier than controls, producing multiple batches of high-quality eggs (average rates of fertilization 66-68% and hatching 55-60%); control fish eggs had significantly lower fertilization (39%) and hatching rates (25%). GnRH-A treatment doubled the egg production, synchronized the females, and shortened the interovulatory periods. The proportion of round, clear, and floating eggs lacking a perivitelline space before fertilization was an indicator of the fertilization (r2= 0.60) and hatching (r2 = 0.56) success. Moreover, egg collections of less than 10 mL varied greatly in quality, whereas larger batches, generally promoted by GnRH-A, consisted of relatively high-quality eggs. GnRH-A treated fish had increased plasma levels of estradiol- 17β 4 days after implantation. A model for prediction of the time between hormone treatment and first spawning (T; days) was developed using the initial follicle diameter (D; μm) as the only predictor (T = 365(D - 380)-082; r2 = 0.91). |
Volckaert, F; Alvarez, M C; Argenton, F; Bonhomme, F; Chatain, B; Colombo, L; Castilho, R; Chevassus, B; Gorshkova, G; Kohler, M; Magoulas, A; Martinez, G; McAndrew, B; Piferrer, F; Vandeputte, M; Zanuy, S Has time come for the genetic management of sea bass? Journal Article Aquaculture Europe, 22 (2), pp. 46–47, 1997. @article{volckaert_has_1997, title = {Has time come for the genetic management of sea bass?}, author = {F Volckaert and M C Alvarez and F Argenton and F Bonhomme and B Chatain and L Colombo and R Castilho and B Chevassus and G Gorshkova and M Kohler and A Magoulas and G Martinez and B McAndrew and F Piferrer and M Vandeputte and S Zanuy}, year = {1997}, date = {1997-01-01}, journal = {Aquaculture Europe}, volume = {22}, number = {2}, pages = {46--47}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Mylonas, C C; Gissis, A; Magnus, Y; Zohar, Y Aquaculture, 153 (3-4), pp. 301–311, 1997, ISSN: 00448486. @article{mylonas_hormonal_1997, title = {Hormonal changes in male white bass (Morone chrysops) and evaluation of milt quality after treatment with a sustained-release GnRHa delivery system}, author = {C C Mylonas and A Gissis and Y Magnus and Y Zohar}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030759556&doi=10.1016%2fS0044-8486%2897%2987566-7&partnerID=40&md5=46bd2d2d502a25d0f3ec6e54a305aba9}, doi = {10.1016/S0044-8486(97)87566-7}, issn = {00448486}, year = {1997}, date = {1997-01-01}, journal = {Aquaculture}, volume = {153}, number = {3-4}, pages = {301--311}, abstract = {Captive white bass, (Morone chrysops) were exposed to an increase in temperature and treated with a gonadotropin-releasing hormone agonist (GnRHa) delivery system during the spermiation period. Circulating levels of various reproductive hormones, and milt production and quality were examined before and after GnRHa treatment. Circulating levels of gonadotropin II (GtH II) prior to GnRHa treatment were below the assay detection limit (0.4 ng ml-1), while mean (±SEM) plasma levels of 17α,20β-dihydroxy-4-pregnen-3-one (17,20β-P) were 0.09 ± 0.03 ng ml-1 testosterone (T) levels were 0,79 ± 0.11 ng ml-1 and 11-ketotestosterone (11-KT) levels were 1.7 ± 0.3 ng ml-1. Treatment with the GnRHa delivery system induced significant increases in plasma GtH 11 and 17,20β-P after 7 days, whereas T and 11-KT were unaffected. Plasma 17α,20β,21-trihydroxy-4-pregnen-3-one (17,20β,21-P) remained unchanged during the experiment with a mean of 0.55 ± 0.05 ng ml-1. Mean total expressible milt increased significantly after treatment with the GnRHa delivery system, from 2.0 ± 0.1 ml kg-1 body weight on day 0 to 3.6 ± 0.5 ml kg-1 on day 7. Milt production remained constant in saline-injected control fish and averaged 2.1 ± 0.2 ml kg-1. Sperm density, motility and fertilization percentage of total inseminated eggs did not differ between milt from GnRHa- and saline-injected fish, and averaged 52 ± 2 x 109 spermatozoa ml-1, 50 ± 4% motile spermatozoa and 60 ± 5% fertilized eggs, respectively. These results demonstrate the effectiveness of sustained GnRHa treatment in enhancing mill production in white bass, without negatively affecting mill quality. It is assumed that enhancement of milt production is caused by high circulating GtH II levels, via the action of 17.20β-P.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Captive white bass, (Morone chrysops) were exposed to an increase in temperature and treated with a gonadotropin-releasing hormone agonist (GnRHa) delivery system during the spermiation period. Circulating levels of various reproductive hormones, and milt production and quality were examined before and after GnRHa treatment. Circulating levels of gonadotropin II (GtH II) prior to GnRHa treatment were below the assay detection limit (0.4 ng ml-1), while mean (±SEM) plasma levels of 17α,20β-dihydroxy-4-pregnen-3-one (17,20β-P) were 0.09 ± 0.03 ng ml-1 testosterone (T) levels were 0,79 ± 0.11 ng ml-1 and 11-ketotestosterone (11-KT) levels were 1.7 ± 0.3 ng ml-1. Treatment with the GnRHa delivery system induced significant increases in plasma GtH 11 and 17,20β-P after 7 days, whereas T and 11-KT were unaffected. Plasma 17α,20β,21-trihydroxy-4-pregnen-3-one (17,20β,21-P) remained unchanged during the experiment with a mean of 0.55 ± 0.05 ng ml-1. Mean total expressible milt increased significantly after treatment with the GnRHa delivery system, from 2.0 ± 0.1 ml kg-1 body weight on day 0 to 3.6 ± 0.5 ml kg-1 on day 7. Milt production remained constant in saline-injected control fish and averaged 2.1 ± 0.2 ml kg-1. Sperm density, motility and fertilization percentage of total inseminated eggs did not differ between milt from GnRHa- and saline-injected fish, and averaged 52 ± 2 x 109 spermatozoa ml-1, 50 ± 4% motile spermatozoa and 60 ± 5% fertilized eggs, respectively. These results demonstrate the effectiveness of sustained GnRHa treatment in enhancing mill production in white bass, without negatively affecting mill quality. It is assumed that enhancement of milt production is caused by high circulating GtH II levels, via the action of 17.20β-P. |
Borsa, P; Naciri, M; Bahri, L; Chikhi, L; de Leon, Garcia F J; Kotoulas, G; Bonhomme, F Infraspecific zoogeography of the Mediterranean: population genetic analysis on sixteen Atlanto-Mediterranean species (fishes and invertebrates) Journal Article Vie et Milieu, 47 , pp. 295 – 305, 1997. @article{borsa_infraspecific_1997-1, title = {Infraspecific zoogeography of the Mediterranean: population genetic analysis on sixteen Atlanto-Mediterranean species (fishes and invertebrates)}, author = {P Borsa and M Naciri and L Bahri and L Chikhi and F J Garcia de Leon and G Kotoulas and F Bonhomme}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0031403021&partnerID=40&md5=84cd00d266e65a028ccbf3c537e40b88}, year = {1997}, date = {1997-01-01}, journal = {Vie et Milieu}, volume = {47}, pages = {295 -- 305}, abstract = {The faunal relationships of the Atlantic and the Mediterranean have been affected by tectonic change in the Miocene, by climate change in the Pleistocene and by hydrological discontinuity. We use a population genetics approach to the biogeography of the Mediterranean marine fauna, in particular to describe the level of recent or present-day exchanges with the Atlantic. The level of genetic differences between populations was estimated from published and unpublished data on sixteen species from the northeastern Atlantic and the Mediterranean. For a majority of species, either tropical or subtropical or boreal fishes, or coastal invertebrates, a moderately strong to strong genetic cline between each side of the Gibraltar strait area was observed. Such a pattern was also observed in a proportion of species between each side of the Sicilian-Tunisian strait. In a limited number of cases, the geographic patterns of population genetic structure however conformed with the expectations from an isolation-by-distance model. The molecular phylogeographic approach in a boreal species, the flounder Platichthys flesus, led to the inferrence of the historical movements of colonisation and geographic isolation of the populations. A similar approach used in a tropical species (the Spanish sardine Sardinella aurita) and a cosmopolitan species (the grey mullet Mugil cephalus) revealed close genetic relationships between Mediterranean and west-tropical Atlantic populations. We analyse the possible implications of these findings to the systematics of the Mediterranean populations and we speculate about the future of boreal species vis-a-vis the present climatic change. We debate on various hypotheses for the maintaining of abrupt genetic clines in the marine environment.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The faunal relationships of the Atlantic and the Mediterranean have been affected by tectonic change in the Miocene, by climate change in the Pleistocene and by hydrological discontinuity. We use a population genetics approach to the biogeography of the Mediterranean marine fauna, in particular to describe the level of recent or present-day exchanges with the Atlantic. The level of genetic differences between populations was estimated from published and unpublished data on sixteen species from the northeastern Atlantic and the Mediterranean. For a majority of species, either tropical or subtropical or boreal fishes, or coastal invertebrates, a moderately strong to strong genetic cline between each side of the Gibraltar strait area was observed. Such a pattern was also observed in a proportion of species between each side of the Sicilian-Tunisian strait. In a limited number of cases, the geographic patterns of population genetic structure however conformed with the expectations from an isolation-by-distance model. The molecular phylogeographic approach in a boreal species, the flounder Platichthys flesus, led to the inferrence of the historical movements of colonisation and geographic isolation of the populations. A similar approach used in a tropical species (the Spanish sardine Sardinella aurita) and a cosmopolitan species (the grey mullet Mugil cephalus) revealed close genetic relationships between Mediterranean and west-tropical Atlantic populations. We analyse the possible implications of these findings to the systematics of the Mediterranean populations and we speculate about the future of boreal species vis-a-vis the present climatic change. We debate on various hypotheses for the maintaining of abrupt genetic clines in the marine environment. |
Borsa, P; Naciri, M; Bahri, L; Chikhi, L; de Leon, Garcia F J; Kotoulas, G; Bonhomme, F Vie et Milieu, 47 (4), pp. 295–305, 1997, ISSN: 02408759. @article{borsa_infraspecific_1997, title = {Infraspecific zoogeography of the Mediterranean: population genetic analysis on sixteen Atlanto-Mediterranean species (fishes and invertebrates) [Zoogeographie infra-specifique de la Mer Mediterranee: analyse des donnees genetiques populationnelles sur seize especes atlanto-mediterraneennes (poissons et invertebres)]}, author = {P Borsa and M Naciri and L Bahri and L Chikhi and F J Garcia de Leon and G Kotoulas and F Bonhomme}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0031403021&partnerID=40&md5=84cd00d266e65a028ccbf3c537e40b88}, issn = {02408759}, year = {1997}, date = {1997-01-01}, journal = {Vie et Milieu}, volume = {47}, number = {4}, pages = {295--305}, abstract = {The faunal relationships of the Atlantic and the Mediterranean have been affected by tectonic change in the Miocene, by climate change in the Pleistocene and by hydrological discontinuity. We use a population genetics approach to the biogeography of the Mediterranean marine fauna, in particular to describe the level of recent or present-day exchanges with the Atlantic. The level of genetic differences between populations was estimated from published and unpublished data on sixteen species from the northeastern Atlantic and the Mediterranean. For a majority of species, either tropical or subtropical or boreal fishes, or coastal invertebrates, a moderately strong to strong genetic cline between each side of the Gibraltar strait area was observed. Such a pattern was also observed in a proportion of species between each side of the Sicilian-Tunisian strait. In a limited number of cases, the geographic patterns of population genetic structure however conformed with the expectations from an isolation-by-distance model. The molecular phylogeographic approach in a boreal species, the flounder Platichthys flesus, led to the inferrence of the historical movements of colonisation and geographic isolation of the populations. A similar approach used in a tropical species (the Spanish sardine Sardinella aurita) and a cosmopolitan species (the grey mullet Mugil cephalus) revealed close genetic relationships between Mediterranean and west-tropical Atlantic populations. We analyse the possible implications of these findings to the systematics of the Mediterranean populations and we speculate about the future of boreal species vis-a-vis the present climatic change. We debate on various hypotheses for the maintaining of abrupt genetic clines in the marine environment.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The faunal relationships of the Atlantic and the Mediterranean have been affected by tectonic change in the Miocene, by climate change in the Pleistocene and by hydrological discontinuity. We use a population genetics approach to the biogeography of the Mediterranean marine fauna, in particular to describe the level of recent or present-day exchanges with the Atlantic. The level of genetic differences between populations was estimated from published and unpublished data on sixteen species from the northeastern Atlantic and the Mediterranean. For a majority of species, either tropical or subtropical or boreal fishes, or coastal invertebrates, a moderately strong to strong genetic cline between each side of the Gibraltar strait area was observed. Such a pattern was also observed in a proportion of species between each side of the Sicilian-Tunisian strait. In a limited number of cases, the geographic patterns of population genetic structure however conformed with the expectations from an isolation-by-distance model. The molecular phylogeographic approach in a boreal species, the flounder Platichthys flesus, led to the inferrence of the historical movements of colonisation and geographic isolation of the populations. A similar approach used in a tropical species (the Spanish sardine Sardinella aurita) and a cosmopolitan species (the grey mullet Mugil cephalus) revealed close genetic relationships between Mediterranean and west-tropical Atlantic populations. We analyse the possible implications of these findings to the systematics of the Mediterranean populations and we speculate about the future of boreal species vis-a-vis the present climatic change. We debate on various hypotheses for the maintaining of abrupt genetic clines in the marine environment. |
Karakassis, Ioannis; Tsapakis, E; Xatziyanni, E; Maidanou, M; Papadopoulou, K -N; Pita, P; Smith, C Interaction of Aquaculture and the Marine Environment. Methodology and preliminary results Journal Article Fisheries News, 192 , pp. 40–45, 1997. @article{karakassis_interaction_1997, title = {Interaction of Aquaculture and the Marine Environment. Methodology and preliminary results}, author = {Ioannis Karakassis and E Tsapakis and E Xatziyanni and M Maidanou and K -N Papadopoulou and P Pita and C Smith}, year = {1997}, date = {1997-01-01}, journal = {Fisheries News}, volume = {192}, pages = {40--45}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Tselepides, A; Papadopoulou, K -N; Podaras, D; Plaiti, W; Koutsoubas, D; Pantazoglou, F Macrobenthic community structure in Cretan Sea. Inproceedings pp. 233–236, Kavala, Greece, 1997, (Publication Title: the 5th National Symposium on Oceanography and Fisheries). @inproceedings{tselepides_macrobenthic_1997, title = {Macrobenthic community structure in Cretan Sea.}, author = {A Tselepides and K -N Papadopoulou and D Podaras and W Plaiti and D Koutsoubas and F Pantazoglou}, year = {1997}, date = {1997-01-01}, pages = {233--236}, address = {Kavala, Greece}, note = {Publication Title: the 5th National Symposium on Oceanography and Fisheries}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Schulz, R W; Wind, Van Der F; Janssen-Dommerholt, C; Peute, J; Mylonas, C C; Zohar, Y; Swanson, P; Goos, H J T Modulation of testicular androgen production in adolescent African catfish (Clarias gariepinus) Journal Article General and Comparative Endocrinology, 108 (1), pp. 56–66, 1997, ISSN: 00166480, (Publisher: Academic Press Inc.). @article{schulz_modulation_1997, title = {Modulation of testicular androgen production in adolescent African catfish (Clarias gariepinus)}, author = {R W Schulz and F Van Der Wind and C Janssen-Dommerholt and J Peute and C C Mylonas and Y Zohar and P Swanson and H J T Goos}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0031260440&doi=10.1006%2fgcen.1997.6951&partnerID=40&md5=13ca91398eaf6fdeffb0cdab1a93756c}, doi = {10.1006/gcen.1997.6951}, issn = {00166480}, year = {1997}, date = {1997-01-01}, journal = {General and Comparative Endocrinology}, volume = {108}, number = {1}, pages = {56--66}, abstract = {At 6 months of age the first spermatozoa appear in the testes of the African catfish considered to be adolescent, since the development to adulthood (12 months of age) is accompanied by further morphological and functional differentiation of Leydig cells. There are increasing plasma levels of 11-ketotestosterone (11-KT) and an increasing responsiveness to luteinizing hormone (LH) of testicular androgen secretion in vitro. Whether treatment of adolescent males with key hormones of the brain-pituitary-gonad axis [gonadotropin-releasing hormone (GnRH), LH, and 11-KT] affects the testicular steroidogenic response to a challenge with LH in vitro 7 days later has been investigated. Injection of GnRH (2.5 μg chicken GnRH-II per kilogram of body weight), LH (25 μg/kg), or a high dose of 11-KT (50 μg/kg) down-regulated basal and LH-stimulated testicular androgen secretion to a minimum of 35% of control values. Treatment with LH was, moreover, associated with changes in the ultrastructure of Leydig cell mitochondria which were either swollen and had a less electron-dense matrix or showed an elongated shape. Conversely, a moderate dose of 11-KT (20 μg/kg) enhanced LH- stimulated, but not basal, androgen secretion in vitro to a maximum of 190% of control values. In view of the generally low LH plasma levels and of the steadily increasing 11-KT plasma levels during puberty, 11-KT may be involved in the up-regulation of the testicular steroidogenic capacity observed during development to full maturity.}, note = {Publisher: Academic Press Inc.}, keywords = {}, pubstate = {published}, tppubtype = {article} } At 6 months of age the first spermatozoa appear in the testes of the African catfish considered to be adolescent, since the development to adulthood (12 months of age) is accompanied by further morphological and functional differentiation of Leydig cells. There are increasing plasma levels of 11-ketotestosterone (11-KT) and an increasing responsiveness to luteinizing hormone (LH) of testicular androgen secretion in vitro. Whether treatment of adolescent males with key hormones of the brain-pituitary-gonad axis [gonadotropin-releasing hormone (GnRH), LH, and 11-KT] affects the testicular steroidogenic response to a challenge with LH in vitro 7 days later has been investigated. Injection of GnRH (2.5 μg chicken GnRH-II per kilogram of body weight), LH (25 μg/kg), or a high dose of 11-KT (50 μg/kg) down-regulated basal and LH-stimulated testicular androgen secretion to a minimum of 35% of control values. Treatment with LH was, moreover, associated with changes in the ultrastructure of Leydig cell mitochondria which were either swollen and had a less electron-dense matrix or showed an elongated shape. Conversely, a moderate dose of 11-KT (20 μg/kg) enhanced LH- stimulated, but not basal, androgen secretion in vitro to a maximum of 190% of control values. In view of the generally low LH plasma levels and of the steadily increasing 11-KT plasma levels during puberty, 11-KT may be involved in the up-regulation of the testicular steroidogenic capacity observed during development to full maturity. |
Alexis, M N; Karanikolas, K K; Richards, R H Pathological findings owing to the lack of ascorbic acid in cultured gilthead bream (Sparus aurata L.) Journal Article Aquaculture, 151 , pp. 209 – 218, 1997. @article{alexis_pathological_1997, title = {Pathological findings owing to the lack of ascorbic acid in cultured gilthead bream (Sparus aurata L.)}, author = {M N Alexis and K K Karanikolas and R H Richards}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0030744502&partnerID=40&md5=a2139d0a7cbd31d6263725d17ad392b7}, year = {1997}, date = {1997-01-01}, journal = {Aquaculture}, volume = {151}, pages = {209 -- 218}, abstract = {The appearance of pathological signs owing to vitamin C deficiency and the wound healing response were followed in experiments with gilthead bream, initial weight 0.5 g, fed with increasing levels of ascorbic acid in the diets. The levels of ascorbic acid used ranged from 0 to 3,2 g kg-1 diet. Certain pathological signs such as extensive tubular damage, glomerulonephritis, and inflammatory response of the haemopoetic tissue producing granuloma and in some cases extending in the nearby tissues and organs, appeared in all fish fed the vitamin C deficient diet, while fish fed the diet supplemented with 50 mg ascorbate per kilogram of diet showed only damage to renal tubules. The gross deficiency signs observed were anorexia, scale loss, depigmentation, internal and external haemorrhages. Mortalities reached a high level after the first month of rearing. The wound healing response showed a direct correlation to ascorbate level in the diet. The unknown etiology of granulomatosis observed by other researchers in cultured seabream may be related to vitamin C deficiency, since in this experiment, it produced the same kidney pathology.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The appearance of pathological signs owing to vitamin C deficiency and the wound healing response were followed in experiments with gilthead bream, initial weight 0.5 g, fed with increasing levels of ascorbic acid in the diets. The levels of ascorbic acid used ranged from 0 to 3,2 g kg-1 diet. Certain pathological signs such as extensive tubular damage, glomerulonephritis, and inflammatory response of the haemopoetic tissue producing granuloma and in some cases extending in the nearby tissues and organs, appeared in all fish fed the vitamin C deficient diet, while fish fed the diet supplemented with 50 mg ascorbate per kilogram of diet showed only damage to renal tubules. The gross deficiency signs observed were anorexia, scale loss, depigmentation, internal and external haemorrhages. Mortalities reached a high level after the first month of rearing. The wound healing response showed a direct correlation to ascorbate level in the diet. The unknown etiology of granulomatosis observed by other researchers in cultured seabream may be related to vitamin C deficiency, since in this experiment, it produced the same kidney pathology. |
Pitta, P; Giannakourou, A; Divanach, P; Kentouri, M Planktonic food web in marine mesocosms in the Eastern Mediterranean: Bottom-up or top-down regulation? Journal Article Hydrobiologia, 363 , pp. 97 – 105, 1997. @article{pitta_planktonic_1997, title = {Planktonic food web in marine mesocosms in the Eastern Mediterranean: Bottom-up or top-down regulation?}, author = {P Pitta and A Giannakourou and P Divanach and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0031402824&partnerID=40&md5=d32feeb1ddf43866aab11765538d6bfa}, year = {1997}, date = {1997-01-01}, journal = {Hydrobiologia}, volume = {363}, pages = {97 -- 105}, abstract = {A mesocosm experiment was conducted in order to study the structure of the planktonic food web. The dynamics of pico-, nano- and microplankton populations were followed during 40 days in four large (40 m3) enclosures. In three tanks a gradient of added nutrients (nitrogen and phosphorus) was applied, while a fourth tank was used as a control. On day 14, the top predator (sea bream Sparus aurata larvae) was introduced into the tanks and part of the water column in each tank was isolated in a plastic bag without fish larvae, to act as a control for predation. Physical parameters, chlorophyll a and nutrient concentrations, as well as plankton concentrations were monitored. A diatom bloom was observed in all four tanks, in the first phase ending with silicate depletion. Flagellate and dinoflagellate abundance subsequently increased, these organisms being limited by zooplankton grazing. The zooplankton populations were controlled by both resources (mostly flagellates) and predation (by fish larvae) as indicated by the results of the control experiments.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A mesocosm experiment was conducted in order to study the structure of the planktonic food web. The dynamics of pico-, nano- and microplankton populations were followed during 40 days in four large (40 m3) enclosures. In three tanks a gradient of added nutrients (nitrogen and phosphorus) was applied, while a fourth tank was used as a control. On day 14, the top predator (sea bream Sparus aurata larvae) was introduced into the tanks and part of the water column in each tank was isolated in a plastic bag without fish larvae, to act as a control for predation. Physical parameters, chlorophyll a and nutrient concentrations, as well as plankton concentrations were monitored. A diatom bloom was observed in all four tanks, in the first phase ending with silicate depletion. Flagellate and dinoflagellate abundance subsequently increased, these organisms being limited by zooplankton grazing. The zooplankton populations were controlled by both resources (mostly flagellates) and predation (by fish larvae) as indicated by the results of the control experiments. |
Mylonas, C C; Scott, A P; Zohar, Y General and Comparative Endocrinology, 108 (2), pp. 223–236, 1997, ISSN: 00166480, (Publisher: Academic Press Inc.). @article{mylonas_plasma_1997, title = {Plasma gonadotropin II, sex steroids, and thyroid hormones in wild striped bass (Morone saxatilis) during spermiation and final oocyte maturation}, author = {C C Mylonas and A P Scott and Y Zohar}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030728062&doi=10.1006%2fgcen.1997.6967&partnerID=40&md5=e2e788f3e08d9b279b44c1e17b96812f}, doi = {10.1006/gcen.1997.6967}, issn = {00166480}, year = {1997}, date = {1997-01-01}, journal = {General and Comparative Endocrinology}, volume = {108}, number = {2}, pages = {223--236}, abstract = {The blood levels of gonadotropin II (GtH II), sex-steroid hormones, and thyroid hormones were determined in wild spermiating male striped bass (Morone saxatilis) in males and in females at various stages of final oocyte maturation (FOM), captured on their spawning grounds. The progression of spermiation was associated with increases in plasma GtH II and decreases in plasma testosterone (T), 11-ketotestosterone, and thyroxine (T4). Plasma triiodothyronine (T3) remained at high and relatively unchanged levels. Plasma levels of 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P) and 17,20β,21-trihydroxy-4-pregnen-3-one (17,20β,21-P), the proposed maturation-inducing steroids (MIS) in striped bass, were low and unchanged during the same period. It was concluded that low progestogen levels are adequate to induce spermiation in striped bass, and that higher levels may be associated with spawning behavior. In the females, based on the profiles of the studied hormones, FOM was separated into two phases. Early FOM, which included germinal vesicle (GV) migration and lipid-droplet coalescence, was associated with elevations in plasma GtH II, T, and estradiol 17β. Late FOM, which included GV breakdown and yolk-globule coalescence, was associated with a further surge in plasma GtH II, increases in the levels of the two MIS, mainly 17,20β-P, and a drop in T4. Plasma T3 levels did not change during FOM. Examination of conjugated steroids demonstrated, in the males, a reduction in conjugated androgens at the peak of the spawning season and, in the females, a small increase in conjugated 17,20β-dihydroxylated and 5β- reduced, 3α-hydroxylated steroids after spawning. This is the most comprehensive report, to date, on the endocrine regulation of gonadal maturation in wild striped bass, demonstrating that a two-stage process of FOM is regulated by different endocrine signals, providing further evidence for the involvement of 17,20β-P as a MIS in the females, and indicating that both males and females are in an euthyroid state during the spawning season.}, note = {Publisher: Academic Press Inc.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The blood levels of gonadotropin II (GtH II), sex-steroid hormones, and thyroid hormones were determined in wild spermiating male striped bass (Morone saxatilis) in males and in females at various stages of final oocyte maturation (FOM), captured on their spawning grounds. The progression of spermiation was associated with increases in plasma GtH II and decreases in plasma testosterone (T), 11-ketotestosterone, and thyroxine (T4). Plasma triiodothyronine (T3) remained at high and relatively unchanged levels. Plasma levels of 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P) and 17,20β,21-trihydroxy-4-pregnen-3-one (17,20β,21-P), the proposed maturation-inducing steroids (MIS) in striped bass, were low and unchanged during the same period. It was concluded that low progestogen levels are adequate to induce spermiation in striped bass, and that higher levels may be associated with spawning behavior. In the females, based on the profiles of the studied hormones, FOM was separated into two phases. Early FOM, which included germinal vesicle (GV) migration and lipid-droplet coalescence, was associated with elevations in plasma GtH II, T, and estradiol 17β. Late FOM, which included GV breakdown and yolk-globule coalescence, was associated with a further surge in plasma GtH II, increases in the levels of the two MIS, mainly 17,20β-P, and a drop in T4. Plasma T3 levels did not change during FOM. Examination of conjugated steroids demonstrated, in the males, a reduction in conjugated androgens at the peak of the spawning season and, in the females, a small increase in conjugated 17,20β-dihydroxylated and 5β- reduced, 3α-hydroxylated steroids after spawning. This is the most comprehensive report, to date, on the endocrine regulation of gonadal maturation in wild striped bass, demonstrating that a two-stage process of FOM is regulated by different endocrine signals, providing further evidence for the involvement of 17,20β-P as a MIS in the females, and indicating that both males and females are in an euthyroid state during the spawning season. |
Tsagkarakou, Anastasia; Navajas, Maria; Lagnel, Jacques; Pasteur, Nicole Population structure in the spider mite Tetranychus urticae (Acari: Tetranychidae) from Crete based on multiple allozymes Journal Article Heredity, 78 (1), pp. 84–92, 1997, ISSN: 0018-067X, 1365-2540. @article{tsagkarakou_population_1997, title = {Population structure in the spider mite Tetranychus urticae (Acari: Tetranychidae) from Crete based on multiple allozymes}, author = {Anastasia Tsagkarakou and Maria Navajas and Jacques Lagnel and Nicole Pasteur}, url = {http://www.nature.com/articles/hdy199710}, doi = {10.1038/hdy.1997.10}, issn = {0018-067X, 1365-2540}, year = {1997}, date = {1997-01-01}, urldate = {2020-08-18}, journal = {Heredity}, volume = {78}, number = {1}, pages = {84--92}, abstract = {The polymorphism of four isozymes was studied on single females of Tetranychus urticae from Crete (Greece), using an isoelectric focusing technique. Genetic differentiation was found to be correlated with distance but not with the species of colonized host-plants. Thus no differentiation was observed between samples collected on citrus trees, tomato, pumpkin, okra or weed plants located within a 50 m2 area, showing that at this geographical scale T. urticae, populations are panmictic. In contrast, samples from plants at 150 m or more from one another displayed a significant genetic differentiation. These results are discussed in relation to the known pattern of migration in the species.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The polymorphism of four isozymes was studied on single females of Tetranychus urticae from Crete (Greece), using an isoelectric focusing technique. Genetic differentiation was found to be correlated with distance but not with the species of colonized host-plants. Thus no differentiation was observed between samples collected on citrus trees, tomato, pumpkin, okra or weed plants located within a 50 m2 area, showing that at this geographical scale T. urticae, populations are panmictic. In contrast, samples from plants at 150 m or more from one another displayed a significant genetic differentiation. These results are discussed in relation to the known pattern of migration in the species. |
Psara, S; Tselepides, A; Ignatiades, L; Dafnomili, E Primary production estimates in the oligotrophic Cretan sea Inproceedings pp. 225–229, 1997, (Publication Title: 5th Pan-Hellenic Symposium of Oceanography and Fisheries). @inproceedings{psara_primary_1997, title = {Primary production estimates in the oligotrophic Cretan sea}, author = {S Psara and A Tselepides and L Ignatiades and E Dafnomili}, year = {1997}, date = {1997-01-01}, volume = {I}, pages = {225--229}, note = {Publication Title: 5th Pan-Hellenic Symposium of Oceanography and Fisheries}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Mylonas, C C; Magnus, Y; Klebanov, Y; Gissis, A; Zohar, Y Reproductive biology and endocrine regulation of final oocyte maturation of captive white bass Journal Article Journal of Fish Biology, 51 (2), pp. 234–250, 1997, ISSN: 00221112. @article{mylonas_reproductive_1997, title = {Reproductive biology and endocrine regulation of final oocyte maturation of captive white bass}, author = {C C Mylonas and Y Magnus and Y Klebanov and A Gissis and Y Zohar}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030741032&doi=10.1006%2fjfbi.1997.0433&partnerID=40&md5=aa2b0d7a5082efd22a1935123608e83a}, doi = {10.1006/jfbi.1997.0433}, issn = {00221112}, year = {1997}, date = {1997-01-01}, journal = {Journal of Fish Biology}, volume = {51}, number = {2}, pages = {234--250}, abstract = {The ovarian development, and plasma levels of gonadotropin II (GtH II) and sex-steroid hormones at the end of vitellogenesis were examined in captive white bass Morone chrysops. The changes in plasma hormone levels and oocyte morphology associated with gonadotropin-releasing hormone agonist (GnRHa)-induced final oocyte maturation (FOM) were studied. Although plasma 17β-oestradiol (E2) and oocyte diameter increased, there were no changes in GtH II, testosterone (T), 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P) or 17,20β,21-dihydroxy-4-pregnen-3-one (17,20β,21-P) in non-hormone-treated females, and no FOM was observed. Treatment with a sustained-release GnRHa delivery system (GnRHa implant) induced two FOM cycles separated by about 24 h, with the release of approximately equal numbers of eggs in each spawn. Plasma GtH II levels were elevated significantly throughout FOM, reaching a maximum of 9.07 ± 1.55 ng ml-1 in ovulated fish. Both plasma E2 and T increased soon after the GnRHa treatment, but E2 declined in fish undergoing germinal vesicle (GV) migration. Plasma T increased further during FOM (7.55 ± 2.87 ng ml-1), but declined precipitously at ovulation. A surge in plasma 17,20β-P and 17,20β,21-P (4.11 ± 0.97 ng ml-1 and 3.10 ± 0.77 ng ml-1, respectively) was observed in females undergoing GV breakdown (GVBD). Based on the involvement of different sex-steroid hormones, FOM was separated into two stages. Early FOM included lipid-droplet coalescence and GV migration, and was associated with elevations in plasma GtH II and T. Late FOM included GVBD and yolk-globule coalescence, and was associated with elevations in plasma GtH II, 17,20β-P and 17,20β,21-P. The results of this study point to the absence of a surge in plasma GtH II as the missing link in the reproductive axis responsible for the failure of captive white bass to undergo FOM at the end of vitellogenesis. Sustained elevation of plasma GtH II via treatment with a GnRHa implant induced two consecutive spawns with an overall egg production two- to eightfold higher than previously obtained from captive broodstocks, and similar to annual egg production values reported for wild fish.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The ovarian development, and plasma levels of gonadotropin II (GtH II) and sex-steroid hormones at the end of vitellogenesis were examined in captive white bass Morone chrysops. The changes in plasma hormone levels and oocyte morphology associated with gonadotropin-releasing hormone agonist (GnRHa)-induced final oocyte maturation (FOM) were studied. Although plasma 17β-oestradiol (E2) and oocyte diameter increased, there were no changes in GtH II, testosterone (T), 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P) or 17,20β,21-dihydroxy-4-pregnen-3-one (17,20β,21-P) in non-hormone-treated females, and no FOM was observed. Treatment with a sustained-release GnRHa delivery system (GnRHa implant) induced two FOM cycles separated by about 24 h, with the release of approximately equal numbers of eggs in each spawn. Plasma GtH II levels were elevated significantly throughout FOM, reaching a maximum of 9.07 ± 1.55 ng ml-1 in ovulated fish. Both plasma E2 and T increased soon after the GnRHa treatment, but E2 declined in fish undergoing germinal vesicle (GV) migration. Plasma T increased further during FOM (7.55 ± 2.87 ng ml-1), but declined precipitously at ovulation. A surge in plasma 17,20β-P and 17,20β,21-P (4.11 ± 0.97 ng ml-1 and 3.10 ± 0.77 ng ml-1, respectively) was observed in females undergoing GV breakdown (GVBD). Based on the involvement of different sex-steroid hormones, FOM was separated into two stages. Early FOM included lipid-droplet coalescence and GV migration, and was associated with elevations in plasma GtH II and T. Late FOM included GVBD and yolk-globule coalescence, and was associated with elevations in plasma GtH II, 17,20β-P and 17,20β,21-P. The results of this study point to the absence of a surge in plasma GtH II as the missing link in the reproductive axis responsible for the failure of captive white bass to undergo FOM at the end of vitellogenesis. Sustained elevation of plasma GtH II via treatment with a GnRHa implant induced two consecutive spawns with an overall egg production two- to eightfold higher than previously obtained from captive broodstocks, and similar to annual egg production values reported for wild fish. |
Publications
1998 |
Direct amplification of length polymorphisms (DALP), or how to get and characterize new genetic markers in many species Journal Article Nucleic Acids Research, 26 (6), pp. 1458–1465, 1998, ISSN: 0305-1048, 1362-4962. |
Amyloodinium occelatum infestation on sharpsnout sea bream, Puntazzo puntazzo cetti Journal Article Bulletin of the European Association of Fish Pathologists, 18 , pp. 198 – 200, 1998. |
Application of molecular markers to aquaculture and broodstock management with special emphasis on microsatellite DNA Journal Article Cahiers Options Mediterrannes, 34 , pp. 153– 168, 1998. |
Biodiversity and the impact of anthropogenic activities in Mediterranean lagoons: The case of Gialova lagoon, SW Greece Journal Article Oebalia, 24 , pp. 77–91, 1998. |
Distribution of floating tar in the Cretan Sea, Eastern Mediterranean, and its relation to the surface circulation Inproceedings Technical University of Crete, Chania Greece, 1998, (Publication Title: 7th International Conference on Natural & Man - Made Hazards). |
General and Comparative Endocrinology, 110 (3), pp. 276–289, 1998, ISSN: 00166480, (Publisher: Academic Press Inc.). |
Genetic markers in marine biology and aquaculture research: when to use what Incollection Agnese, J F (Ed.): Genetics and Aquaculture in Africa, pp. 67–78, ORSTOM, Paris, 1998, (Backup Publisher: ORSTOM). |
General and Comparative Endocrinology, 112 (2), pp. 163–177, 1998, ISSN: 00166480, (Publisher: Academic Press Inc.). |
Hormone profiles of captive striped bass Morone saxatilis during spermiation, and long-term enhancement of milt production Journal Article Journal of the World Aquaculture Society, 29 (4), pp. 379–392, 1998, ISSN: 08938849, (Publisher: World Aquaculture Society). |
Life history of Maurolicus muelleri in fjordic and oceanic environments Journal Article Journal of Fish Biology, 53 (6), pp. 1324–1341, 1998, ISSN: 00221112. |
Microsatellite sequences are under-represented in two mite genomes Journal Article Insect Molecular Biology, 7 , pp. 249 – 256, 1998. |
Molecular determinants involved in function and specificity of nucleobase transporters are conserved in bacteria, fungi, plants and metazoa Inproceedings Proceedings of the 16th SMYTE, Costa-Papiernicka, Slovakia, 1998, (Publication Title: Proceedings of the 16th SMYTE, Small Meeting on Yeast Transport and Energetics Type: Conference Paper). |
Monitoring 20 years of increased grazing impact on the Greek island of Crete with earth observation satellites Journal Article Journal of Arid Environments, 39 (2), pp. 165–178, 1998, ISSN: 01401963, (Publisher: Academic Press). |
Morphometric characters in gilthead sea bream, Sparus aurata, red porgy, Pagrus pagrus and their hybrids (Sparidae) Journal Article Italian Journal of Zoology, 65 (sup1), pp. 435 – 439, 1998, ISSN: 1125-0003, 1748-5851. |
Natural vs. mariculture induced variability in nutrients and plankton in the eastern Mediterranean Journal Article Hydrobiologia, 391 (1/3), pp. 179–192, 1998, ISSN: 00188158. |
Negative covariance indicates mutation bias in a two-locus microsatellite system in the fish Sparus aurata Journal Article Genetics, 150 , pp. 1567–1575, 1998. |
Pelagic tar, dissolved/dispersed petroleum hydrocarbons and plastic distribution in the Cretan Sea, Greece Journal Article Marine Pollution Bulletin, 36 (12), pp. 989–993, 1998, ISSN: 0025326X. |
Ring formation on otoliths and scales of Pagrus pagrus: A comparative study Journal Article Journal of Fish Biology, 52 , pp. 350 – 361, 1998. |
Stress-related pathology seems a significant obstacle for the intensive farming of common dentex, Dentex dentex (linnaeus 1758) Journal Article Bulletin of the European Association of Fish Pathologists, 18 , pp. 15 – 18, 1998. |
The macrofaunal assemblage associated with the scleractinian coral Cladocora caespitosa (L.) in the Aegean Sea Journal Article Annales de l'Institute Oceanographique, 74 (2), pp. 97–114, 1998, ISSN: 00789682. |
Three polymorphic microsatellites in the Japanese oyster, Crassostrea gigas (Thunberg) Journal Article Animal Genetics, 29 , pp. 69 – 70, 1998. |
1997 |
The opercular complex deformity in intensive gilthead sea bream (Sparus aurata L.) larviculture. Moment of apparition and description Journal Article Aquaculture, 156 (1-2), pp. 165–177, 1997, ISSN: 00448486. |
Fatty acids in muscle of wild and farmed red porgy, Pagrus pagrus Journal Article Aquaculture Nutrition, 3 (3), pp. 161–165, 1997, ISSN: 1353-5773, 1365-2095. |
Protein and carbon utilization of rotifers (Brachionus plicatilis) in first feeding of turbot larvae (Scophthalmus maximus L.) Journal Article Aquaculture, 153 (1-2), pp. 103–122, 1997, ISSN: 00448486. |
Normal and abnormal osteological development of caudal fin in Sparus aurata L. fry Journal Article Aquaculture, 149 (3-4), pp. 215–226, 1997, ISSN: 00448486. |
Aquaculture impact on the planktonic system in three fish farms Inproceedings pp. 219–222, 1997, (Publication Title: 5th Pan-Hellenic Symposium of Oceanography and Fisheries). |
Ceratomyxa spp. (myxosporea) infection in cultured common dentex from Greece Journal Article Bulletin of the European Association of Fish Pathologists, 17 , pp. 174 – 176, 1997. |
Biology of Reproduction, 57 (3), pp. 669–675, 1997, ISSN: 00063363. |
Construction of an artificial reef park and creation of a marine protected zone in order to prevent fishing activities off the coasts of Thrace (N. Aegean Sea) Journal Article Fishing News, 197 , pp. 50–55, 1997, ((in Greek)). |
Cyto-histological examination of post-vitellogenesis and final oocyte maturation in captive-reared striped bass Journal Article Journal of Fish Biology, 50 (1), pp. 34–49, 1997, ISSN: 00221112. |
Diel pattern of haematocrit, serum metabolites, osmotic pressure, electrolytes and thyroid hormones in sea bass and sea bream Journal Article Aquaculture International, 5 , pp. 237 – 247, 1997. |
Distribution of nutrients, chloroplastic pigments, POC, PON and ATP in the Cretan Sea (NE Mediterranean): Seasonal and interannual variability Inproceedings pp. 189–193, 1997, (Publication Title: The 5th Pan-Hellenic Symposium of Oceanography and Fisheries). |
Dynamics of the physicochemical parameters in water column and in the sediments of the Gialova lagoon (NW Peloponnese, Ionian Sea) Inproceedings pp. 351–353, Kavala, Greece, 1997, (Publication Title: Seventh Symposium on Oceanography and Fisheries, Κavala). |
Aquaculture, 158 (1-2), pp. 129–140, 1997, ISSN: 00448486. |
Aquaculture, 156 , pp. 145 – 155, 1997. |
Effects of aquaculture in three coastal areas Journal Article Fisheries News, 199 , pp. 90–95, 1997. |
Canadian Journal of Fisheries and Aquatic Sciences, 54 (9), pp. 1957–1964, 1997, ISSN: 0706652X. |
Has time come for the genetic management of sea bass? Journal Article Aquaculture Europe, 22 (2), pp. 46–47, 1997. |
Aquaculture, 153 (3-4), pp. 301–311, 1997, ISSN: 00448486. |
Infraspecific zoogeography of the Mediterranean: population genetic analysis on sixteen Atlanto-Mediterranean species (fishes and invertebrates) Journal Article Vie et Milieu, 47 , pp. 295 – 305, 1997. |
Vie et Milieu, 47 (4), pp. 295–305, 1997, ISSN: 02408759. |
Interaction of Aquaculture and the Marine Environment. Methodology and preliminary results Journal Article Fisheries News, 192 , pp. 40–45, 1997. |
Macrobenthic community structure in Cretan Sea. Inproceedings pp. 233–236, Kavala, Greece, 1997, (Publication Title: the 5th National Symposium on Oceanography and Fisheries). |
Modulation of testicular androgen production in adolescent African catfish (Clarias gariepinus) Journal Article General and Comparative Endocrinology, 108 (1), pp. 56–66, 1997, ISSN: 00166480, (Publisher: Academic Press Inc.). |
Pathological findings owing to the lack of ascorbic acid in cultured gilthead bream (Sparus aurata L.) Journal Article Aquaculture, 151 , pp. 209 – 218, 1997. |
Planktonic food web in marine mesocosms in the Eastern Mediterranean: Bottom-up or top-down regulation? Journal Article Hydrobiologia, 363 , pp. 97 – 105, 1997. |
General and Comparative Endocrinology, 108 (2), pp. 223–236, 1997, ISSN: 00166480, (Publisher: Academic Press Inc.). |
Population structure in the spider mite Tetranychus urticae (Acari: Tetranychidae) from Crete based on multiple allozymes Journal Article Heredity, 78 (1), pp. 84–92, 1997, ISSN: 0018-067X, 1365-2540. |
Primary production estimates in the oligotrophic Cretan sea Inproceedings pp. 225–229, 1997, (Publication Title: 5th Pan-Hellenic Symposium of Oceanography and Fisheries). |
Reproductive biology and endocrine regulation of final oocyte maturation of captive white bass Journal Article Journal of Fish Biology, 51 (2), pp. 234–250, 1997, ISSN: 00221112. |