2021 |
Digenis, Markos; Ragkousis, Michail; Vasileiadou, Katerina; Gerovasileiou, Vasilis; Katsanevakis, Stelios New records of the Indo-Pacific shrimp Urocaridella pulchella Yokeş & Galil, 2006 from the Eastern Mediterranean Sea Journal Article BIR, 10 (2), pp. 295–303, 2021, ISSN: 22421300. @article{digenis_new_2021, title = {New records of the Indo-Pacific shrimp Urocaridella pulchella Yokeş & Galil, 2006 from the Eastern Mediterranean Sea}, author = {Markos Digenis and Michail Ragkousis and Katerina Vasileiadou and Vasilis Gerovasileiou and Stelios Katsanevakis}, url = {https://www.reabic.net/journals/bir/2021/Issue2.aspx https://imbbc.hcmr.gr/wp-content/uploads/2021/05/2021-Digenis-BioInvasions-Records-35.pdf}, doi = {10.3391/bir.2021.10.2.07}, issn = {22421300}, year = {2021}, date = {2021-01-01}, urldate = {2021-05-10}, journal = {BIR}, volume = {10}, number = {2}, pages = {295--303}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Koulouri, Panayota; Mogias, Athanasios; Mokos, Melita; Cheimonopoulou, Maria; Realdon, Giulia; Boubonari, Theodora; Previati, Monica; Formoso, Alba Tojeiro; Kideys, Ahmet Erkan; Hassaan, Mohamed Aly; Patti, Patrizia; Korfiatis, Kostas; Fabri, Sandra; Juan, Xavier Mediterranean Marine Science, 2021, ISSN: 1791-6763, 1108-393X. @article{koulouri_ocean_2021, title = {Ocean Literacy across the Mediterranean Sea basin: Evaluating Middle School Students’ Knowledge, Attitudes, and Behaviour towards Ocean Sciences Issues}, author = {Panayota Koulouri and Athanasios Mogias and Melita Mokos and Maria Cheimonopoulou and Giulia Realdon and Theodora Boubonari and Monica Previati and Alba Tojeiro Formoso and Ahmet Erkan Kideys and Mohamed Aly Hassaan and Patrizia Patti and Kostas Korfiatis and Sandra Fabri and Xavier Juan}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/05/2022-Koulouri-MedMarSci-30.pdf https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/26797}, doi = {10.12681/mms.26797}, issn = {1791-6763, 1108-393X}, year = {2021}, date = {2021-01-01}, urldate = {2022-05-09}, journal = {Mediterranean Marine Science}, abstract = {The Mediterranean Sea is characterized by rich biodiversity, and its region hosts people living in several countries with a rich variety of cultures, but – at the same time – it is “under siege”, due to anthropogenic pressures. To address these pressures, many actions are needed aiming, among others, at establishing Ocean Literacy (OL) across the Mediterranean countries and preparing the future generation of Mediterranean Sea-literate citizens. Towards this aim, the present cross-national study investigated OL issues in relation to content knowledge, possible common misconceptions, attitudes, and the self-reported behavior of 2,533 middle school students from eight Mediterranean countries (Croatia, Cyprus, Egypt, Greece, Italy, Malta, Spain, and Turkey), as well as certain background elements (e.g., gender, grade level, environmental education experience, sources of relevant information). The results of this study revealed that middle school students of all studied countries possess a moderate level of ocean sciences content knowledge, while they showed satisfactory pro-environmental attitudes and behavior. These findings along with further research are expected to function as a baseline for the design, implementation, and launch of specifically targeted programs, educational activities, teaching resources, curricula, and school textbooks, which will be achieved through close collaboration between schools, universities, research institutes, and Ministries of Education, thus contributing to the future protection and sustainable development of the Mediterranean Sea region.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The Mediterranean Sea is characterized by rich biodiversity, and its region hosts people living in several countries with a rich variety of cultures, but – at the same time – it is “under siege”, due to anthropogenic pressures. To address these pressures, many actions are needed aiming, among others, at establishing Ocean Literacy (OL) across the Mediterranean countries and preparing the future generation of Mediterranean Sea-literate citizens. Towards this aim, the present cross-national study investigated OL issues in relation to content knowledge, possible common misconceptions, attitudes, and the self-reported behavior of 2,533 middle school students from eight Mediterranean countries (Croatia, Cyprus, Egypt, Greece, Italy, Malta, Spain, and Turkey), as well as certain background elements (e.g., gender, grade level, environmental education experience, sources of relevant information). The results of this study revealed that middle school students of all studied countries possess a moderate level of ocean sciences content knowledge, while they showed satisfactory pro-environmental attitudes and behavior. These findings along with further research are expected to function as a baseline for the design, implementation, and launch of specifically targeted programs, educational activities, teaching resources, curricula, and school textbooks, which will be achieved through close collaboration between schools, universities, research institutes, and Ministries of Education, thus contributing to the future protection and sustainable development of the Mediterranean Sea region. |
Eparkhina, D; Pomaro, A; Koulouri, Panayota; Banchi, E; Canu, D; Uyarra, M; Burke, N Brussels, Belgium, 2021, (BIODIV). @book{eparkhina_ocean_2021, title = {Ocean Literacy in European Oceanographic Agencies: EuroGOOS recommendations for the UN Decade of Ocean Science for Sustainable Development 2021-2030}, author = {D Eparkhina and A Pomaro and Panayota Koulouri and E Banchi and D Canu and M Uyarra and N Burke}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/05/EuroGOOS_Ocean_Literacy_Policy_Brief_2021-1.pdf https://eurogoos.eu/download/ocean-literacy-in-european-oceanographic-agencies/?wpdmdl=10078&refresh=61f162cca08571643209420}, year = {2021}, date = {2021-01-01}, address = {Brussels, Belgium}, series = {EuroGOOS Policy Brief}, note = {BIODIV}, keywords = {}, pubstate = {published}, tppubtype = {book} } |
Fanini, Lucia; Guittard, Alice On single use plastic straws: Pre-ban findings on touristic beaches in Crete Journal Article Marine Pollution Bulletin, 171 , pp. 112790, 2021, ISSN: 0025326X. @article{fanini_single_2021, title = {On single use plastic straws: Pre-ban findings on touristic beaches in Crete}, author = {Lucia Fanini and Alice Guittard}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/08/2021-Fanini-MarPollBull-58-pre-print.pdf https://linkinghub.elsevier.com/retrieve/pii/S0025326X21008249 }, doi = {10.1016/j.marpolbul.2021.112790}, issn = {0025326X}, year = {2021}, date = {2021-01-01}, urldate = {2021-08-23}, journal = {Marine Pollution Bulletin}, volume = {171}, pages = {112790}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Papapetrou, Maria; Kazlari, Zoi; Papanna, Kantham; Papaharisis, Leonidas; Oikonomou, Stavroula; Manousaki, Tereza; Loukovitis, Dimitrios; Kottaras, Lefteris; Dimitroglou, Arkadios; Gourzioti, Evgenia; Pagonis, Charalampos; Kostandis, Andreas; Tsigenopoulos, Costas S; Chatziplis, Dimitrios Aquaculture Reports, 20 , pp. 100767, 2021, ISSN: 23525134. @article{papapetrou_trail_2021, title = {On the trail of detecting genetic (co)variation between resistance to parasite infections (Diplectanum aequans and Lernanthropus kroyeri) and growth in European seabass (Dicentrarchus labrax)}, author = {Maria Papapetrou and Zoi Kazlari and Kantham Papanna and Leonidas Papaharisis and Stavroula Oikonomou and Tereza Manousaki and Dimitrios Loukovitis and Lefteris Kottaras and Arkadios Dimitroglou and Evgenia Gourzioti and Charalampos Pagonis and Andreas Kostandis and Costas S Tsigenopoulos and Dimitrios Chatziplis}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/06/2021-Papapetrou-AquaRep-46.pdf https://linkinghub.elsevier.com/retrieve/pii/S2352513421001836}, doi = {10.1016/j.aqrep.2021.100767}, issn = {23525134}, year = {2021}, date = {2021-01-01}, urldate = {2021-06-24}, journal = {Aquaculture Reports}, volume = {20}, pages = {100767}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Demetriou, Monica; Raitsos, Dionysios E; Kournopoulou, Antonia; Mandalakis, Manolis; Sfenthourakis, Spyros; Psarra, Stella Phytoplankton Phenology in the Coastal Zone of Cyprus, Based on Remote Sensing and In Situ Observations Journal Article Remote Sensing, 14 (1), pp. 12, 2021, ISSN: 2072-4292. @article{demetriou_phytoplankton_2021, title = {Phytoplankton Phenology in the Coastal Zone of Cyprus, Based on Remote Sensing and In Situ Observations}, author = {Monica Demetriou and Dionysios E Raitsos and Antonia Kournopoulou and Manolis Mandalakis and Spyros Sfenthourakis and Stella Psarra}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/01/2022-Demetriou-RemSensing-4.pdf https://www.mdpi.com/2072-4292/14/1/12}, doi = {10.3390/rs14010012}, issn = {2072-4292}, year = {2021}, date = {2021-01-01}, urldate = {2022-01-12}, journal = {Remote Sensing}, volume = {14}, number = {1}, pages = {12}, abstract = {Alterations in phytoplankton biomass, community structure and timing of their growth (phenology), are directly implicated in the carbon cycle and energy transfer to higher trophic levels of the marine food web. Due to the lack of long-term in situ datasets, there is very little information on phytoplankton seasonal succession in Cyprus (eastern Mediterranean Sea). On the other hand, satellite-derived measurements of ocean colour can only provide long-term time series of chlorophyll (an index of phytoplankton biomass) up to the first optical depth (surface waters). The coupling of both means of observations is essential for understanding phytoplankton dynamics and their response to environmental change. Here, we use 23 years of remotely sensed, regionally tuned ocean-colour observations, along with a unique time series of in situ phytoplankton pigment composition data, collected in coastal waters of Cyprus during 2016. The satellite observations show an initiation of phytoplankton growth period in November, a peak in February and termination in April, with an overall mean duration of textasciitilde4 months. An in-depth exploration of in situ total Chl-a concentration and phytoplankton pigments revealed that pico- and nano-plankton cells dominated the phytoplankton community. The growth peak in February was dominated by nanophytoplankton and potentially larger diatoms (pigments of 19’ hexanoyloxyfucoxanthin and fucoxanthin, respectively), in the 0–20 m layer. The highest total Chl-a concentration was recorded at a station off Akrotiri peninsula in the south, where strong coastal upwelling has been reported. Another station in the southern part, located next to a fish farm, showed a higher contribution of picophytoplankton during the most oligotrophic period (summer). Our results highlight the importance of using available in situ data coupled to ocean-colour remote sensing, for monitoring marine ecosystems in areas with limited in situ data availability.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Alterations in phytoplankton biomass, community structure and timing of their growth (phenology), are directly implicated in the carbon cycle and energy transfer to higher trophic levels of the marine food web. Due to the lack of long-term in situ datasets, there is very little information on phytoplankton seasonal succession in Cyprus (eastern Mediterranean Sea). On the other hand, satellite-derived measurements of ocean colour can only provide long-term time series of chlorophyll (an index of phytoplankton biomass) up to the first optical depth (surface waters). The coupling of both means of observations is essential for understanding phytoplankton dynamics and their response to environmental change. Here, we use 23 years of remotely sensed, regionally tuned ocean-colour observations, along with a unique time series of in situ phytoplankton pigment composition data, collected in coastal waters of Cyprus during 2016. The satellite observations show an initiation of phytoplankton growth period in November, a peak in February and termination in April, with an overall mean duration of textasciitilde4 months. An in-depth exploration of in situ total Chl-a concentration and phytoplankton pigments revealed that pico- and nano-plankton cells dominated the phytoplankton community. The growth peak in February was dominated by nanophytoplankton and potentially larger diatoms (pigments of 19’ hexanoyloxyfucoxanthin and fucoxanthin, respectively), in the 0–20 m layer. The highest total Chl-a concentration was recorded at a station off Akrotiri peninsula in the south, where strong coastal upwelling has been reported. Another station in the southern part, located next to a fish farm, showed a higher contribution of picophytoplankton during the most oligotrophic period (summer). Our results highlight the importance of using available in situ data coupled to ocean-colour remote sensing, for monitoring marine ecosystems in areas with limited in situ data availability. |
Riechers, Maraja; Fanini, Lucia; Apicella, Annalisa; Galván, Carolina Batista; Blondel, Elise; Espiña, Begoña; Kefer, Simone; Keroullé, Tristan; Klun, Katja; Pereira, Tania R; Ronchi, Francesca; Rodríguez, Pedro Ruiz; Sardon, Haritz; Silva, Alexandra Viana; Stulgis, Maris; Ibarra-González, Nagore Plastics in our ocean as transdisciplinary challenge Journal Article Marine Pollution Bulletin, 164 , pp. 112051, 2021, ISSN: 0025326X. @article{riechers_plastics_2021, title = {Plastics in our ocean as transdisciplinary challenge}, author = {Maraja Riechers and Lucia Fanini and Annalisa Apicella and Carolina Batista Galván and Elise Blondel and Begoña Espiña and Simone Kefer and Tristan Keroullé and Katja Klun and Tania R Pereira and Francesca Ronchi and Pedro Ruiz Rodríguez and Haritz Sardon and Alexandra Viana Silva and Maris Stulgis and Nagore Ibarra-González}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0025326X21000850}, doi = {10.1016/j.marpolbul.2021.112051}, issn = {0025326X}, year = {2021}, date = {2021-01-01}, urldate = {2021-02-24}, journal = {Marine Pollution Bulletin}, volume = {164}, pages = {112051}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Mente, Eleni; Carter, Chris G; Barnes, Robin (Katersky) S; Vlahos, Nikolaos; Nengas, Ioannis Post-Prandial Amino Acid Changes in Gilthead Sea Bream Journal Article Animals, 11 (7), pp. 1889, 2021, ISSN: 2076-2615. @article{mente_post-prandial_2021, title = {Post-Prandial Amino Acid Changes in Gilthead Sea Bream}, author = {Eleni Mente and Chris G Carter and Robin (Katersky) S Barnes and Nikolaos Vlahos and Ioannis Nengas}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/07/2021-Mente-Animals-54.pdf https://www.mdpi.com/2076-2615/11/7/1889}, doi = {10.3390/ani11071889}, issn = {2076-2615}, year = {2021}, date = {2021-01-01}, urldate = {2021-07-26}, journal = {Animals}, volume = {11}, number = {7}, pages = {1889}, abstract = {Following a meal, a series of physiological changes occurs in fish as they digest, absorb and assimilate ingested nutrients. This study aims to assess post-prandial free amino acid (FAA) activity in gilthead sea bream consuming a partial marine protein (fishmeal) replacement. Sea bream were fed diets where 16 and 27% of the fishmeal protein was replaced by plant protein. The essential amino acid (EAA) composition of the white muscle, liver and gut of sea bream was strongly correlated with the EAA composition of the 16% protein replacement diet compared to the 27% protein replacement diet. The mean FAA concentration in the white muscle and liver changed at 4 to 8 h after a meal and was not different to pre-feeding (0 h) and at 24 h after feeding. It was confirmed in this study that 16% replacement of marine protein with plant protein meets the amino acid needs of sea bream. Overall, the present study contributes towards understanding post-prandial amino acid profiles during uptake, tissue assimilation and immediate metabolic processing of amino acids in sea bream consuming a partial marine protein replacement. This study suggests the need to further investigate the magnitude of the post-prandial tissue-specific amino acid activity in relation to species-specific abilities to regulate metabolism due to dietary nutrient utilization.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Following a meal, a series of physiological changes occurs in fish as they digest, absorb and assimilate ingested nutrients. This study aims to assess post-prandial free amino acid (FAA) activity in gilthead sea bream consuming a partial marine protein (fishmeal) replacement. Sea bream were fed diets where 16 and 27% of the fishmeal protein was replaced by plant protein. The essential amino acid (EAA) composition of the white muscle, liver and gut of sea bream was strongly correlated with the EAA composition of the 16% protein replacement diet compared to the 27% protein replacement diet. The mean FAA concentration in the white muscle and liver changed at 4 to 8 h after a meal and was not different to pre-feeding (0 h) and at 24 h after feeding. It was confirmed in this study that 16% replacement of marine protein with plant protein meets the amino acid needs of sea bream. Overall, the present study contributes towards understanding post-prandial amino acid profiles during uptake, tissue assimilation and immediate metabolic processing of amino acids in sea bream consuming a partial marine protein replacement. This study suggests the need to further investigate the magnitude of the post-prandial tissue-specific amino acid activity in relation to species-specific abilities to regulate metabolism due to dietary nutrient utilization. |
Amar, Yacine; Lagkouvardos, Ilias; Silva, Rafaela L; Ishola, Oluwaseun Ayodeji; Foesel, Bärbel U; Kublik, Susanne; Schöler, Anne; Niedermeier, Sebastian; Bleuel, Rachela; Zink, Alexander; Neuhaus, Klaus; Schloter, Michael; Biedermann, Tilo; Köberle, Martin Pre-digest of unprotected DNA by Benzonase improves the representation of living skin bacteria and efficiently depletes host DNA Journal Article Microbiome, 9 (1), pp. 123, 2021, ISSN: 2049-2618. @article{amar_pre-digest_2021, title = {Pre-digest of unprotected DNA by Benzonase improves the representation of living skin bacteria and efficiently depletes host DNA}, author = {Yacine Amar and Ilias Lagkouvardos and Rafaela L Silva and Oluwaseun Ayodeji Ishola and Bärbel U Foesel and Susanne Kublik and Anne Schöler and Sebastian Niedermeier and Rachela Bleuel and Alexander Zink and Klaus Neuhaus and Michael Schloter and Tilo Biedermann and Martin Köberle}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/07/2021-Amar-Microbiome-55.pdf https://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-021-01067-0}, doi = {10.1186/s40168-021-01067-0}, issn = {2049-2618}, year = {2021}, date = {2021-01-01}, urldate = {2021-07-26}, journal = {Microbiome}, volume = {9}, number = {1}, pages = {123}, abstract = {Abstract Background The identification of microbiota based on next-generation sequencing (NGS) of extracted DNA has drastically improved our understanding of the role of microbial communities in health and disease. However, DNA-based microbiome analysis cannot per se differentiate between living and dead microorganisms. In environments such as the skin, host defense mechanisms including antimicrobial peptides and low cutaneous pH result in a high microbial turnover, likely resulting in high numbers of dead cells present and releasing substantial amounts of microbial DNA. NGS analyses may thus lead to inaccurate estimations of microbiome structures and consequently functional capacities. Results We investigated in this study the feasibility of a Benzonase-based approach (BDA) to pre-digest unprotected DNA, i.e., of dead microbial cells, as a method to overcome these limitations, thus offering a more accurate assessment of the living microbiome. A skin mock community as well as skin microbiome samples were analyzed using 16S rRNA gene sequencing and metagenomics sequencing after DNA extraction with and without a Benzonase digest to assess bacterial diversity patterns. The BDA method resulted in less reads from dead bacteria both in the skin mock community and skin swabs spiked with either heat-inactivated bacteria or bacterial-free DNA. This approach also efficiently depleted host DNA reads in samples with high human-to-microbial DNA ratios, with no obvious impact on the microbiome profile. We further observed that low biomass samples generate an α-diversity bias when the bacterial load is lower than 10 5 CFU and that Benzonase digest is not sufficient to overcome this bias. Conclusions The BDA approach enables both a better assessment of the living microbiota and depletion of host DNA reads. Graphical abstract}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Background The identification of microbiota based on next-generation sequencing (NGS) of extracted DNA has drastically improved our understanding of the role of microbial communities in health and disease. However, DNA-based microbiome analysis cannot per se differentiate between living and dead microorganisms. In environments such as the skin, host defense mechanisms including antimicrobial peptides and low cutaneous pH result in a high microbial turnover, likely resulting in high numbers of dead cells present and releasing substantial amounts of microbial DNA. NGS analyses may thus lead to inaccurate estimations of microbiome structures and consequently functional capacities. Results We investigated in this study the feasibility of a Benzonase-based approach (BDA) to pre-digest unprotected DNA, i.e., of dead microbial cells, as a method to overcome these limitations, thus offering a more accurate assessment of the living microbiome. A skin mock community as well as skin microbiome samples were analyzed using 16S rRNA gene sequencing and metagenomics sequencing after DNA extraction with and without a Benzonase digest to assess bacterial diversity patterns. The BDA method resulted in less reads from dead bacteria both in the skin mock community and skin swabs spiked with either heat-inactivated bacteria or bacterial-free DNA. This approach also efficiently depleted host DNA reads in samples with high human-to-microbial DNA ratios, with no obvious impact on the microbiome profile. We further observed that low biomass samples generate an α-diversity bias when the bacterial load is lower than 10 5 CFU and that Benzonase digest is not sufficient to overcome this bias. Conclusions The BDA approach enables both a better assessment of the living microbiota and depletion of host DNA reads. Graphical abstract |
Wenderlein, Jasmin; Böswald, Linda F; Ulrich, Sebastian; Kienzle, Ellen; Neuhaus, Klaus; Lagkouvardos, Ilias; Zenner, Christian; Straubinger, Reinhard K Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Microbiome Journal Article Animals, 11 (3), pp. 862, 2021, ISSN: 2076-2615. @article{wenderlein_processing_2021, title = {Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Microbiome}, author = {Jasmin Wenderlein and Linda F Böswald and Sebastian Ulrich and Ellen Kienzle and Klaus Neuhaus and Ilias Lagkouvardos and Christian Zenner and Reinhard K Straubinger}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/07/2021-Wanderlein-animals-48.pdf https://www.mdpi.com/2076-2615/11/3/862}, doi = {10.3390/ani11030862}, issn = {2076-2615}, year = {2021}, date = {2021-01-01}, urldate = {2021-07-26}, journal = {Animals}, volume = {11}, number = {3}, pages = {862}, abstract = {The composition of the microbiome is subject to the host’s diet. In commercial laboratory mouse diets, different physical forms of the same diets are available, containing—according to their labels—identical ingredients and nutrient compositions. However, variations in nutrient composition and starch gelatinization due to production processes and their impact on digestibility have been described. In this study, a total of 48 C57BL/J6 mice were assigned to two equal groups and were fed diets (produced with different processes—extruded vs. pelleted) for eight weeks in two biological replicates. At the end of the experiment, samples were collected from five different gastrointestinal regions, including the stomach, small intestine, cecum, large intestine, and an extracorporeal region (feces), and the microbiome was analyzed with 16S rRNA gene amplicon sequencing. The replicates in both experiments differed significantly in their relative abundances of Muribaculaceae species. Furthermore, the gastrointestinal content of pellet-fed mice contained larger numbers of Lactobacillus species. These results indicate that starch gelatinization and ingredient composition significantly influence microbial makeup. In conclusion, different feed processing methods may affect fundamental digestive and metabolic processes, impacting animal experiments and biasing microbiome data.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The composition of the microbiome is subject to the host’s diet. In commercial laboratory mouse diets, different physical forms of the same diets are available, containing—according to their labels—identical ingredients and nutrient compositions. However, variations in nutrient composition and starch gelatinization due to production processes and their impact on digestibility have been described. In this study, a total of 48 C57BL/J6 mice were assigned to two equal groups and were fed diets (produced with different processes—extruded vs. pelleted) for eight weeks in two biological replicates. At the end of the experiment, samples were collected from five different gastrointestinal regions, including the stomach, small intestine, cecum, large intestine, and an extracorporeal region (feces), and the microbiome was analyzed with 16S rRNA gene amplicon sequencing. The replicates in both experiments differed significantly in their relative abundances of Muribaculaceae species. Furthermore, the gastrointestinal content of pellet-fed mice contained larger numbers of Lactobacillus species. These results indicate that starch gelatinization and ingredient composition significantly influence microbial makeup. In conclusion, different feed processing methods may affect fundamental digestive and metabolic processes, impacting animal experiments and biasing microbiome data. |
Stavrakidis-Zachou, Orestis; Lika, Konstadia; Anastasiadis, Panagiotis; Papandroulakis, Nikos Projecting climate change impacts on Mediterranean finfish production: a case study in Greece Journal Article Climatic Change, 165 (3), pp. 67, 2021, ISSN: 1573-1480. @article{stavrakidis-zachou_projecting_2021, title = {Projecting climate change impacts on Mediterranean finfish production: a case study in Greece}, author = {Orestis Stavrakidis-Zachou and Konstadia Lika and Panagiotis Anastasiadis and Nikos Papandroulakis}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/06/2021-Stavrakidis-Zachou-Climate-Chnage-38.pdf https://doi.org/10.1007/s10584-021-03096-y}, doi = {10.1007/s10584-021-03096-y}, issn = {1573-1480}, year = {2021}, date = {2021-01-01}, urldate = {2021-06-24}, journal = {Climatic Change}, volume = {165}, number = {3}, pages = {67}, abstract = {Finfish aquaculture in the Mediterranean Sea faces increasing challenges due to climate change, while potential adaptation requires a robust assessment of the arising threats and opportunities. This paper presents an approach developed to investigate effects of climate drivers on Greek aquaculture, a representative Mediterranean country with a leading role in the sector. Using a farm level approach, dynamic energy budget models for European seabass and meagre were developed, and environmental forcing was used to simulate changes in production and farm profitability under IPCC scenarios RCP45 and RCP85. The effects of temperature and extreme weather events at the individual and farm levels were considered along with that of husbandry parameters such as stocking timing, market size, and farm location (inshore, offshore) for nine regions. The simulations suggest that at the individual level, fish may benefit from warmer temperatures in the future in terms of growth, thus reaching commercial sizes faster, while the husbandry parameters may have as large an effect on growth as the projected shifts in climatic cues. However, this benefit will be largely offset by the adverse effects of extreme weather events at the population level. Such events will be more frequent in the future and, depending on the intensity one assigns to them, they could cause losses in biomass and farm profits that range from mild to detrimental for the industry. Overall, these results provide quantification of some of the potential threats for an important aquaculture sector while suggesting possibilities to benefit from emerging opportunities. Therefore, they could contribute to improving the sector’s readiness for tackling important challenges in the future.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Finfish aquaculture in the Mediterranean Sea faces increasing challenges due to climate change, while potential adaptation requires a robust assessment of the arising threats and opportunities. This paper presents an approach developed to investigate effects of climate drivers on Greek aquaculture, a representative Mediterranean country with a leading role in the sector. Using a farm level approach, dynamic energy budget models for European seabass and meagre were developed, and environmental forcing was used to simulate changes in production and farm profitability under IPCC scenarios RCP45 and RCP85. The effects of temperature and extreme weather events at the individual and farm levels were considered along with that of husbandry parameters such as stocking timing, market size, and farm location (inshore, offshore) for nine regions. The simulations suggest that at the individual level, fish may benefit from warmer temperatures in the future in terms of growth, thus reaching commercial sizes faster, while the husbandry parameters may have as large an effect on growth as the projected shifts in climatic cues. However, this benefit will be largely offset by the adverse effects of extreme weather events at the population level. Such events will be more frequent in the future and, depending on the intensity one assigns to them, they could cause losses in biomass and farm profits that range from mild to detrimental for the industry. Overall, these results provide quantification of some of the potential threats for an important aquaculture sector while suggesting possibilities to benefit from emerging opportunities. Therefore, they could contribute to improving the sector’s readiness for tackling important challenges in the future. |
Hitch, Thomas C A; Afrizal, Afrizal; Riedel, Thomas; Kioukis, Antonios; Haller, Dirk; Lagkouvardos, Ilias; Overmann, Jörg; Clavel, Thomas Recent advances in culture-based gut microbiome research Journal Article International Journal of Medical Microbiology, 311 (3), pp. 151485, 2021, ISSN: 14384221. @article{hitch_recent_2021, title = {Recent advances in culture-based gut microbiome research}, author = {Thomas C A Hitch and Afrizal Afrizal and Thomas Riedel and Antonios Kioukis and Dirk Haller and Ilias Lagkouvardos and Jörg Overmann and Thomas Clavel}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/07/2021-Hitch-IJMM-49.pdf https://linkinghub.elsevier.com/retrieve/pii/S143842212100014X}, doi = {10.1016/j.ijmm.2021.151485}, issn = {14384221}, year = {2021}, date = {2021-01-01}, urldate = {2021-07-26}, journal = {International Journal of Medical Microbiology}, volume = {311}, number = {3}, pages = {151485}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Karagiannidis, Efstratios; Papazoglou, Andreas S; Stalikas, Nikolaos; Deda, Olga; Panteris, Eleftherios; Begou, Olga; Sofidis, Georgios; Moysidis, Dimitrios V; Kartas, Anastasios; Chatzinikolaou, Evangelia; Keklikoglou, Kleoniki; Bompoti, Andreana; Gika, Helen; Theodoridis, Georgios; Sianos, Georgios Serum Ceramides as Prognostic Biomarkers of Large Thrombus Burden in Patients with STEMI: A Micro-Computed Tomography Study Journal Article Journal of Personalized Medicine, 11 (2), pp. 89, 2021. @article{karagiannidis_serum_2021, title = {Serum Ceramides as Prognostic Biomarkers of Large Thrombus Burden in Patients with STEMI: A Micro-Computed Tomography Study}, author = {Efstratios Karagiannidis and Andreas S Papazoglou and Nikolaos Stalikas and Olga Deda and Eleftherios Panteris and Olga Begou and Georgios Sofidis and Dimitrios V Moysidis and Anastasios Kartas and Evangelia Chatzinikolaou and Kleoniki Keklikoglou and Andreana Bompoti and Helen Gika and Georgios Theodoridis and Georgios Sianos}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/02/2021-Karagiannidis-Chatzinikolaou-Keklikoglou-JPMed-9.pdf https://www.mdpi.com/2075-4426/11/2/89}, doi = {10.3390/jpm11020089}, year = {2021}, date = {2021-01-01}, urldate = {2021-02-05}, journal = {Journal of Personalized Medicine}, volume = {11}, number = {2}, pages = {89}, abstract = {ST-elevation myocardial infarction (STEMI) remains one of the leading causes of mortality worldwide. The identification of novel metabolic and imaging biomarkers could unveil key pathophysiological mechanisms at the molecular level and promote personalized care in patients with acute coronary syndromes. We studied 38 patients with STEMI who underwent primary percutaneous coronary intervention and thrombus aspiration. We sought to correlate serum ceramide levels with micro-CT quantified aspirated thrombus volume and relevant angiographic outcomes, including modified TIMI thrombus grade and pre- or post-procedural TIMI flow. Higher ceramide C16:0 levels were significantly but weakly correlated with larger aspirated thrombus volume (Spearman r = 0.326}, keywords = {}, pubstate = {published}, tppubtype = {article} } ST-elevation myocardial infarction (STEMI) remains one of the leading causes of mortality worldwide. The identification of novel metabolic and imaging biomarkers could unveil key pathophysiological mechanisms at the molecular level and promote personalized care in patients with acute coronary syndromes. We studied 38 patients with STEMI who underwent primary percutaneous coronary intervention and thrombus aspiration. We sought to correlate serum ceramide levels with micro-CT quantified aspirated thrombus volume and relevant angiographic outcomes, including modified TIMI thrombus grade and pre- or post-procedural TIMI flow. Higher ceramide C16:0 levels were significantly but weakly correlated with larger aspirated thrombus volume (Spearman r = 0.326 |
Puzenat, Valentine; Escartín, Javier; Martelat, Jean-Emmanuel; Barreyre, Thibaut; Bauer, Sven Le Moine; Nomikou, Paraskevi; Gracias, Nuno; Allemand, Pascal; Antoniou, Varvara; Coskun, Omer; Garcia, Rafael; Grandjean, Philippe; Jørgensen, Steffen Leth; Magí, Lluís; Mandalakis, Manolis; Orsi, William; Polymenakou, Paraskevi; Schouw, Anders; Vallicrosa, Guillem; Vlasopoulos, Othonas Shallow-water hydrothermalism at Milos (Greece): Nature, distribution, heat fluxes and impact on ecosystems Journal Article Marine Geology, 438 , pp. 106521, 2021, ISSN: 00253227. @article{puzenat_shallow-water_2021, title = {Shallow-water hydrothermalism at Milos (Greece): Nature, distribution, heat fluxes and impact on ecosystems}, author = {Valentine Puzenat and Javier Escartín and Jean-Emmanuel Martelat and Thibaut Barreyre and Sven Le Moine Bauer and Paraskevi Nomikou and Nuno Gracias and Pascal Allemand and Varvara Antoniou and Omer Coskun and Rafael Garcia and Philippe Grandjean and Steffen Leth Jørgensen and Lluís Magí and Manolis Mandalakis and William Orsi and Paraskevi Polymenakou and Anders Schouw and Guillem Vallicrosa and Othonas Vlasopoulos}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0025322721001031}, doi = {10.1016/j.margeo.2021.106521}, issn = {00253227}, year = {2021}, date = {2021-01-01}, urldate = {2022-02-22}, journal = {Marine Geology}, volume = {438}, pages = {106521}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Superio, Joshua; Fakriadis, Ioannis; Tsigenopoulos, Costas S; Lancerotto, Stefano Adam; Rodriguez, Andrea Villena; Vervelakis, Emanuele; Mylonas, Constantinos C Spawning kinetics and parentage contribution of European sea bass (Dicentrarchus labrax) broodstocks, and influence of GnRHa-induced spawning Journal Article Aquaculture Reports, 21 , pp. 100766, 2021, ISSN: 23525134. @article{superio_spawning_2021, title = {Spawning kinetics and parentage contribution of European sea bass (Dicentrarchus labrax) broodstocks, and influence of GnRHa-induced spawning}, author = {Joshua Superio and Ioannis Fakriadis and Costas S Tsigenopoulos and Stefano Adam Lancerotto and Andrea Villena Rodriguez and Emanuele Vervelakis and Constantinos C Mylonas}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/07/2021-Superio-AquaRep-47.pdf https://linkinghub.elsevier.com/retrieve/pii/S2352513421001824}, doi = {10.1016/j.aqrep.2021.100766}, issn = {23525134}, year = {2021}, date = {2021-01-01}, urldate = {2021-07-26}, journal = {Aquaculture Reports}, volume = {21}, pages = {100766}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Fakriadis, Ioannis; Mylonas, Constantinos C Fish Physiology and Biochemistry, 47 (2), pp. 281–292, 2021, ISSN: 1573-5168. @article{fakriadis_sperm_2021, title = {Sperm quality of greater amberjack Seriola dumerili throughout the reproductive season and in response to GnRHa treatment with controlled release implants}, author = {Ioannis Fakriadis and Constantinos C Mylonas}, url = {https://doi.org/10.1007/s10695-020-00910-9}, doi = {10.1007/s10695-020-00910-9}, issn = {1573-5168}, year = {2021}, date = {2021-01-01}, urldate = {2021-04-27}, journal = {Fish Physiology and Biochemistry}, volume = {47}, number = {2}, pages = {281--292}, abstract = {A wild-caught stock of greater amberjack Seriola dumerili reared in sea cages in two commercial aquaculture facilities in Greece was administered gonadotropin-releasing hormone agonist (GnRHa), loaded in controlled release implants, and was used in spawning induction experiments throughout the reproductive season. Sperm quality was evaluated using computer-assisted sperm analysis (CASA) before and after GnRHa administration and the extent of the spermiation period was determined. Males were in spermiation throughout the monitoring period from May 30 until July 18, at temperatures between 19 and 24 °C. However, lower sperm motility duration, density, and survival under cold storage were observed from early July onwards. Sperm quality did not recover after the end of spawning induction experiments in tanks and the return of the fish for 14–28 days to the sea cage in mid-July, which could be related to the high temperatures of this period. An improvement trend was observed in the quality of the milt collected on day 7 after a single GnRHa administration, but a significant decrease was observed on day 21 in sperm density, survival under cold storage, and straight line velocity (VSL). On the contrary, a double GnRHa administration spaced 14 days apart maintained the same sperm quality for a longer period of 29 days. Further spermiation enhancement experiments should be conducted in the future to describe in more detail the kinetics of sperm production after GnRHa therapy and its effects on sperm quality.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A wild-caught stock of greater amberjack Seriola dumerili reared in sea cages in two commercial aquaculture facilities in Greece was administered gonadotropin-releasing hormone agonist (GnRHa), loaded in controlled release implants, and was used in spawning induction experiments throughout the reproductive season. Sperm quality was evaluated using computer-assisted sperm analysis (CASA) before and after GnRHa administration and the extent of the spermiation period was determined. Males were in spermiation throughout the monitoring period from May 30 until July 18, at temperatures between 19 and 24 °C. However, lower sperm motility duration, density, and survival under cold storage were observed from early July onwards. Sperm quality did not recover after the end of spawning induction experiments in tanks and the return of the fish for 14–28 days to the sea cage in mid-July, which could be related to the high temperatures of this period. An improvement trend was observed in the quality of the milt collected on day 7 after a single GnRHa administration, but a significant decrease was observed on day 21 in sperm density, survival under cold storage, and straight line velocity (VSL). On the contrary, a double GnRHa administration spaced 14 days apart maintained the same sperm quality for a longer period of 29 days. Further spermiation enhancement experiments should be conducted in the future to describe in more detail the kinetics of sperm production after GnRHa therapy and its effects on sperm quality. |
Bitner, M A; Gerovasileiou, V Taxonomic composition and assemblage structure of brachiopods from two submarine caves in the Aegean Sea, Eastern Mediterranean Journal Article The European Zoological Journal, 88 (1), pp. 316–327, 2021, ISSN: 2475-0263. @article{bitner_taxonomic_2021, title = {Taxonomic composition and assemblage structure of brachiopods from two submarine caves in the Aegean Sea, Eastern Mediterranean}, author = {M A Bitner and V Gerovasileiou}, url = {https://www.tandfonline.com/doi/full/10.1080/24750263.2021.1887947 https://imbbc.hcmr.gr/wp-content/uploads/2021/03/2021-Bitner-Gerovasileiou-25.pdf}, doi = {10.1080/24750263.2021.1887947}, issn = {2475-0263}, year = {2021}, date = {2021-01-01}, urldate = {2021-03-17}, journal = {The European Zoological Journal}, volume = {88}, number = {1}, pages = {316--327}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Yildiz, Hijran Yavuzcan; Chatzifotis, Stavros; Anastasiadis, Panagiotis; Parisi, Giuliana; Papandroulakis, Nikos Testing of the Salmon Welfare Index Model (SWIM 1.0) as a computational welfare assessment for sea-caged European sea bass Journal Article Italian Journal of Animal Science, 20 (1), pp. 1423–1430, 2021, ISSN: null. @article{yavuzcan_yildiz_testing_2021, title = {Testing of the Salmon Welfare Index Model (SWIM 1.0) as a computational welfare assessment for sea-caged European sea bass}, author = {Hijran Yavuzcan Yildiz and Stavros Chatzifotis and Panagiotis Anastasiadis and Giuliana Parisi and Nikos Papandroulakis}, url = {https://doi.org/10.1080/1828051X.2021.1961106}, doi = {10.1080/1828051X.2021.1961106}, issn = {null}, year = {2021}, date = {2021-01-01}, urldate = {2022-02-28}, journal = {Italian Journal of Animal Science}, volume = {20}, number = {1}, pages = {1423--1430}, abstract = {The overall fish welfare in sea-caged European sea bass (Dicentrarchus labrax) was quantitatively assessed by adapting the Salmon Welfare Index Model (SWIM 1.0). In the model, a total of 16 operational welfare indicators in three segments (i) individual fish-based; (ii) fish-group specific; and (iii) sea cage environment-specific indicators were evaluated. In this study, the welfare indicators for salmon aquaculture which are incompatible with the characteristics of European sea bass were modified. Differently from SWIM 1.0, the welfare indicators of smoltification and salinity were eliminated; however, gill integrity and pH were added in the modified model of this study while the water temperature attributes were also changed in the model proposed. The modified model was tested for two sea cage farms of European sea bass (Farm A and Farm B) in two different locations of Mediterranean Sea for the first time. The overall welfare scores were expressed by a scale from 0-poor to 1-good welfare. The overall welfare assessment calculated by the model yielded the value of 1.0 and 0.81 for Farm A and Farm B, respectively. Based on the scores, Farm A represented the conditions of ‘good welfare’ while Farm B gave fair warning about welfare status. The methodology developed in the SWIM 1.0 model can be applied to European sea bass in Mediterranean aquaculture with small modifications.HIGHLIGHTSSalmon Welfare Index Model (SWIM 1.0) was utilised for E. sea bass welfare evaluationSWIM 1.0 was tested in sea-caged fish from two farms located in Mediterranean SeaSWIM 1.0 model can be applied to E. sea bass with small modifications}, keywords = {}, pubstate = {published}, tppubtype = {article} } The overall fish welfare in sea-caged European sea bass (Dicentrarchus labrax) was quantitatively assessed by adapting the Salmon Welfare Index Model (SWIM 1.0). In the model, a total of 16 operational welfare indicators in three segments (i) individual fish-based; (ii) fish-group specific; and (iii) sea cage environment-specific indicators were evaluated. In this study, the welfare indicators for salmon aquaculture which are incompatible with the characteristics of European sea bass were modified. Differently from SWIM 1.0, the welfare indicators of smoltification and salinity were eliminated; however, gill integrity and pH were added in the modified model of this study while the water temperature attributes were also changed in the model proposed. The modified model was tested for two sea cage farms of European sea bass (Farm A and Farm B) in two different locations of Mediterranean Sea for the first time. The overall welfare scores were expressed by a scale from 0-poor to 1-good welfare. The overall welfare assessment calculated by the model yielded the value of 1.0 and 0.81 for Farm A and Farm B, respectively. Based on the scores, Farm A represented the conditions of ‘good welfare’ while Farm B gave fair warning about welfare status. The methodology developed in the SWIM 1.0 model can be applied to European sea bass in Mediterranean aquaculture with small modifications.HIGHLIGHTSSalmon Welfare Index Model (SWIM 1.0) was utilised for E. sea bass welfare evaluationSWIM 1.0 was tested in sea-caged fish from two farms located in Mediterranean SeaSWIM 1.0 model can be applied to E. sea bass with small modifications |
Zafeiropoulos, Haris; Gargan, Laura; Hintikka, Sanni; Pavloudi, Christina; Carlsson, Jens The Dark mAtteR iNvestigator (DARN) tool: getting to know the known unknowns in COI amplicon data Journal Article Metabarcoding and Metagenomics, 5 , pp. e69657, 2021, ISSN: 2534-9708. @article{zafeiropoulos_dark_2021, title = {The Dark mAtteR iNvestigator (DARN) tool: getting to know the known unknowns in COI amplicon data}, author = {Haris Zafeiropoulos and Laura Gargan and Sanni Hintikka and Christina Pavloudi and Jens Carlsson}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/12/2021-Zafeiropoulos-ΜΒΜΓ-81.pdf https://mbmg.pensoft.net/article/69657/}, doi = {10.3897/mbmg.5.69657}, issn = {2534-9708}, year = {2021}, date = {2021-01-01}, urldate = {2021-12-01}, journal = {Metabarcoding and Metagenomics}, volume = {5}, pages = {e69657}, abstract = {The mitochondrial cytochrome C oxidase subunit I gene (COI) is commonly used in environmental DNA (eDNA) metabarcoding studies, especially for assessing metazoan diversity. Yet, a great number of COI operational taxonomic units (OTUs) or/and amplicon sequence variants (ASVs) retrieved from such studies do not get a taxonomic assignment with a reference sequence. To assess and investigate such sequences, we have developed the Dark mAtteR iNvestigator (DARN) software tool. For this purpose, a reference COI-oriented phylogenetic tree was built from 1,593 consensus sequences covering all the three domains of life. With respect to eukaryotes, consensus sequences at the family level were constructed from 183,330 sequences retrieved from the Midori reference 2 database, which represented 70% of the initial number of reference sequences. Similarly, sequences from 431 bacterial and 15 archaeal taxa at the family level (29% and 1% of the initial number of reference sequences respectively) were retrieved from the BOLD and the PFam databases. DARN makes use of this phylogenetic tree to investigate COI pre-processed sequences of amplicon samples to provide both a tabular and a graphical overview of their phylogenetic assignments. To evaluate DARN, both environmental and bulk metabarcoding samples from different aquatic environments using various primer sets were analysed. We demonstrate that a large proportion of non-target prokaryotic organisms, such as bacteria and archaea, are also amplified in eDNA samples and we suggest prokaryotic COI sequences to be included in the reference databases used for the taxonomy assignment to allow for further analyses of dark matter. DARN source code is available on GitHub at https://github.com/hariszaf/darn and as a Docker image at https://hub.docker.com/r/hariszaf/darn.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The mitochondrial cytochrome C oxidase subunit I gene (COI) is commonly used in environmental DNA (eDNA) metabarcoding studies, especially for assessing metazoan diversity. Yet, a great number of COI operational taxonomic units (OTUs) or/and amplicon sequence variants (ASVs) retrieved from such studies do not get a taxonomic assignment with a reference sequence. To assess and investigate such sequences, we have developed the Dark mAtteR iNvestigator (DARN) software tool. For this purpose, a reference COI-oriented phylogenetic tree was built from 1,593 consensus sequences covering all the three domains of life. With respect to eukaryotes, consensus sequences at the family level were constructed from 183,330 sequences retrieved from the Midori reference 2 database, which represented 70% of the initial number of reference sequences. Similarly, sequences from 431 bacterial and 15 archaeal taxa at the family level (29% and 1% of the initial number of reference sequences respectively) were retrieved from the BOLD and the PFam databases. DARN makes use of this phylogenetic tree to investigate COI pre-processed sequences of amplicon samples to provide both a tabular and a graphical overview of their phylogenetic assignments. To evaluate DARN, both environmental and bulk metabarcoding samples from different aquatic environments using various primer sets were analysed. We demonstrate that a large proportion of non-target prokaryotic organisms, such as bacteria and archaea, are also amplified in eDNA samples and we suggest prokaryotic COI sequences to be included in the reference databases used for the taxonomy assignment to allow for further analyses of dark matter. DARN source code is available on GitHub at https://github.com/hariszaf/darn and as a Docker image at https://hub.docker.com/r/hariszaf/darn. |
Mokos, Melita; Cheimonopoulou, Maria; Koulouri, Panayota; Previati, Monica; Realdon, Giulia; Santoro, Francesca; Mogias, Athanasios; Boubonari, Theodora; Satta, Alessio; Ioakeimidis, Christos The Importance of Ocean Literacy in the Mediterranean Region—Steps Towards Blue Sustainability Incollection Koutsopoulos, Kostis C; Stel, Jan H (Ed.): Ocean Literacy: Understanding the Ocean, pp. 197–240, Springer International Publishing, Cham, 2021, ISBN: 978-3-030-70154-3 978-3-030-70155-0, (Series Title: Key Challenges in Geography). @incollection{koutsopoulos_importance_2021, title = {The Importance of Ocean Literacy in the Mediterranean Region—Steps Towards Blue Sustainability}, author = {Melita Mokos and Maria Cheimonopoulou and Panayota Koulouri and Monica Previati and Giulia Realdon and Francesca Santoro and Athanasios Mogias and Theodora Boubonari and Alessio Satta and Christos Ioakeimidis}, editor = {Kostis C Koutsopoulos and Jan H Stel}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/05/Mokos-et-al._revised-chapter.pdf}, doi = {10.1007/978-3-030-70155-0_9}, isbn = {978-3-030-70154-3 978-3-030-70155-0}, year = {2021}, date = {2021-01-01}, urldate = {2022-05-15}, booktitle = {Ocean Literacy: Understanding the Ocean}, pages = {197--240}, publisher = {Springer International Publishing}, address = {Cham}, note = {Series Title: Key Challenges in Geography}, keywords = {}, pubstate = {published}, tppubtype = {incollection} } |
Mello, Paulo De H; Lancerotto, Stefano; Fakriadis, Ioannis; Tsoukali, Panagiota; Papadaki, Maria; Mylonas, Constantinos Chrysanthos Mediterranean Marine Science, 22 (2), pp. 218–231, 2021, ISSN: 1791-6763. @article{mello_importance_2021, title = {The importance of thermoperiod for proper gametogenesis and successful egg and sperm production in meagre (Argyrosomus regius) breeders in aquaculture}, author = {Paulo De H Mello and Stefano Lancerotto and Ioannis Fakriadis and Panagiota Tsoukali and Maria Papadaki and Constantinos Chrysanthos Mylonas}, url = {https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/25806 https://imbbc.hcmr.gr/wp-content/uploads/2021/05/2021-De-Mello-MMS-27-2.pdf}, doi = {10.12681/mms.25806}, issn = {1791-6763}, year = {2021}, date = {2021-01-01}, urldate = {2021-04-27}, journal = {Mediterranean Marine Science}, volume = {22}, number = {2}, pages = {218--231}, abstract = {We examined the effect of constant water temperature throughout the year on gametogenesis, spawning success and egg/sperm/embryo quality in meagre (Argyrosomus regius). Two broodstocks were exposed to simulated natural photoperiod, andeither attenuated seasonal water temperature (SeasT, 16.4 to 19.6ºC) or relatively constant water temperature (CoT, 19.4 ± 0.6ºC).In the spawning period (May), 4 couples per group were induced to spawn with gonadotropin releasing hormone agonist (GnRHa). Gonadal stage of development, sperm quality parameters and plasma levels of sex steroids were evaluated prior to the GnRHa treatment. Spawning success and egg/sperm quality were examined over the following 4 weeks. Constant temperature did not prevent gametogenesis, but exposure to attenuated seasonal water temperature with the inclusion of winter low temperature was beneficial to both sexes. The mean (±SD) diameter of the largest vitellogenic oocytes prior to GnRHa administration was significantly higher in the SeasT compared to the CoT group (598 ± 27 vs 520 ± 17 μm). Testosterone plasma levels in the females were significantly higher in the SeasT group, but all other hormones were similar in both sexes. SeasT females spawned more consistently with higher relative fecundity, and 24-h embryo survival of the produced eggs. A more pronounced negative effect of constant water temperature was observed in males, since CoT males exhibited a spermiation index of 0 prior to GnRHa treatment, the latter clearly having a beneficial effect over the following 4 weeks. The study demonstrated that meagre do undergo gametogenesis to a significant extent even under constant water temperatures during the year. However, a seasonal thermal regime -even an attenuated one- was necessary for the proper development of the gametes, allowing for the successful spawning induction using the established GnRHa induction protocol.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We examined the effect of constant water temperature throughout the year on gametogenesis, spawning success and egg/sperm/embryo quality in meagre (Argyrosomus regius). Two broodstocks were exposed to simulated natural photoperiod, andeither attenuated seasonal water temperature (SeasT, 16.4 to 19.6ºC) or relatively constant water temperature (CoT, 19.4 ± 0.6ºC).In the spawning period (May), 4 couples per group were induced to spawn with gonadotropin releasing hormone agonist (GnRHa). Gonadal stage of development, sperm quality parameters and plasma levels of sex steroids were evaluated prior to the GnRHa treatment. Spawning success and egg/sperm quality were examined over the following 4 weeks. Constant temperature did not prevent gametogenesis, but exposure to attenuated seasonal water temperature with the inclusion of winter low temperature was beneficial to both sexes. The mean (±SD) diameter of the largest vitellogenic oocytes prior to GnRHa administration was significantly higher in the SeasT compared to the CoT group (598 ± 27 vs 520 ± 17 μm). Testosterone plasma levels in the females were significantly higher in the SeasT group, but all other hormones were similar in both sexes. SeasT females spawned more consistently with higher relative fecundity, and 24-h embryo survival of the produced eggs. A more pronounced negative effect of constant water temperature was observed in males, since CoT males exhibited a spermiation index of 0 prior to GnRHa treatment, the latter clearly having a beneficial effect over the following 4 weeks. The study demonstrated that meagre do undergo gametogenesis to a significant extent even under constant water temperatures during the year. However, a seasonal thermal regime -even an attenuated one- was necessary for the proper development of the gametes, allowing for the successful spawning induction using the established GnRHa induction protocol. |
Hermi, Fatma; Gómez-Abellán, Victoria; Pérez-Oliva, Ana B; García-Moreno, Diana; López-Muñoz, Azucena; Sarropoulou, Elena; Arizcun, Marta; Ridha, Oueslati; Mulero, Victoriano; Sepulcre, María P Developmental & Comparative Immunology, 114 , pp. 103803, 2021, ISSN: 0145305X. @article{hermi_molecular_2021, title = {The molecular, functional and phylogenetic characterization of PGE2 receptors reveals their different roles in the immune response of the teleost fish gilthead seabream (Sparus aurata L.)}, author = {Fatma Hermi and Victoria Gómez-Abellán and Ana B Pérez-Oliva and Diana García-Moreno and Azucena López-Muñoz and Elena Sarropoulou and Marta Arizcun and Oueslati Ridha and Victoriano Mulero and María P Sepulcre}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0145305X2030358X}, doi = {10.1016/j.dci.2020.103803}, issn = {0145305X}, year = {2021}, date = {2021-01-01}, urldate = {2020-08-23}, journal = {Developmental & Comparative Immunology}, volume = {114}, pages = {103803}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Fanini, Lucia; Marchetti, Giovanni M; Serafeimidou, Ioanna; Papadopoulou, Olympia The potential contribution of bloggers to change lifestyle and reduce plastic use and pollution: A small data approach Journal Article Marine Pollution Bulletin, 169 , pp. 112525, 2021, ISSN: 0025326X. @article{fanini_potential_2021, title = {The potential contribution of bloggers to change lifestyle and reduce plastic use and pollution: A small data approach}, author = {Lucia Fanini and Giovanni M Marchetti and Ioanna Serafeimidou and Olympia Papadopoulou}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0025326X21005592}, doi = {10.1016/j.marpolbul.2021.112525}, issn = {0025326X}, year = {2021}, date = {2021-01-01}, urldate = {2021-06-24}, journal = {Marine Pollution Bulletin}, volume = {169}, pages = {112525}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Polymenakou, Paraskevi N; Nomikou, Paraskevi; Zafeiropoulos, Haris; Mandalakis, Manolis; Anastasiou, Thekla I; Kilias, Stephanos; Kyrpides, Nikos C; Kotoulas, Georgios; Magoulas, Antoniοs The Santorini Volcanic Complex as a Valuable Source of Enzymes for Bioenergy Journal Article Energies, 14 (5), pp. 1414, 2021, ISSN: 1996-1073. @article{polymenakou_santorini_2021, title = {The Santorini Volcanic Complex as a Valuable Source of Enzymes for Bioenergy}, author = {Paraskevi N Polymenakou and Paraskevi Nomikou and Haris Zafeiropoulos and Manolis Mandalakis and Thekla I Anastasiou and Stephanos Kilias and Nikos C Kyrpides and Georgios Kotoulas and Antoniοs Magoulas}, url = {https://www.mdpi.com/1996-1073/14/5/1414 https://imbbc.hcmr.gr/wp-content/uploads/2021/03/2021-Polymenakou-ENERGIES-24.pdf}, doi = {10.3390/en14051414}, issn = {1996-1073}, year = {2021}, date = {2021-01-01}, urldate = {2021-03-17}, journal = {Energies}, volume = {14}, number = {5}, pages = {1414}, abstract = {Marine microbial communities are an untapped reservoir of genetic and metabolic diversity and a valuable source for the discovery of new natural products of biotechnological interest. The newly discovered hydrothermal vent field of Santorini volcanic complex located in the Aegean Sea is gaining increasing interest for potential biotechnological exploitation. The conditions in these environments, i.e., high temperatures, low pH values and high concentration of heavy metals, often resemble harsh industrial settings. Thus, these environments may serve as pools of enzymes of enhanced catalytic properties that may provide benefits to biotechnology. Here, we screened 11 metagenomic libraries previously constructed from microbial mat samples covering the seafloor and the polymetallic chimneys of Kolumbo volcano as well as mat samples from Santorini caldera, to mine, in silico, genes associated with bioenergy applications. We particularly focused on genes encoding biomass hydrolysis enzymes such as cellulases, hemicellulases and lignin-degrading enzymes. A total of 10,417 genes were found for three specific groups of enzymes—i.e., the endoglucanases, the three different beta-glucosidases BGL, bglX and bglB, and the alpha-galactosidases melA, and rafA. Overall, we concluded that the Santorini–Kolumbo volcanic ecosystems constitute a significant resource of novel genes with potential applications in bioenergy that deserve further investigation.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Marine microbial communities are an untapped reservoir of genetic and metabolic diversity and a valuable source for the discovery of new natural products of biotechnological interest. The newly discovered hydrothermal vent field of Santorini volcanic complex located in the Aegean Sea is gaining increasing interest for potential biotechnological exploitation. The conditions in these environments, i.e., high temperatures, low pH values and high concentration of heavy metals, often resemble harsh industrial settings. Thus, these environments may serve as pools of enzymes of enhanced catalytic properties that may provide benefits to biotechnology. Here, we screened 11 metagenomic libraries previously constructed from microbial mat samples covering the seafloor and the polymetallic chimneys of Kolumbo volcano as well as mat samples from Santorini caldera, to mine, in silico, genes associated with bioenergy applications. We particularly focused on genes encoding biomass hydrolysis enzymes such as cellulases, hemicellulases and lignin-degrading enzymes. A total of 10,417 genes were found for three specific groups of enzymes—i.e., the endoglucanases, the three different beta-glucosidases BGL, bglX and bglB, and the alpha-galactosidases melA, and rafA. Overall, we concluded that the Santorini–Kolumbo volcanic ecosystems constitute a significant resource of novel genes with potential applications in bioenergy that deserve further investigation. |
Psonis, Nikolaos; Antoniou, Aglaia; Karameta, Emmanouela; Darriba, Diego; Stamatakis, Alexandros; Lymberakis, Petros; Poulakakis, Nikos The wall lizards of the Balkan peninsula: Tackling questions at the interface of phylogenomics and population genomics Journal Article Molecular Phylogenetics and Evolution, 159 , pp. 107121, 2021, ISSN: 10557903. @article{psonis_wall_2021, title = {The wall lizards of the Balkan peninsula: Tackling questions at the interface of phylogenomics and population genomics}, author = {Nikolaos Psonis and Aglaia Antoniou and Emmanouela Karameta and Diego Darriba and Alexandros Stamatakis and Petros Lymberakis and Nikos Poulakakis}, url = {https://linkinghub.elsevier.com/retrieve/pii/S1055790321000543}, doi = {10.1016/j.ympev.2021.107121}, issn = {10557903}, year = {2021}, date = {2021-01-01}, urldate = {2021-03-12}, journal = {Molecular Phylogenetics and Evolution}, volume = {159}, pages = {107121}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Samuel, Robyn M; Meyer, Raissa; Buttigieg, Pier Luigi; Davies, Neil; Jeffery, Nicholas W; Meyer, Christopher; Pavloudi, Christina; Pitz, Kathleen Johnson; Sweetlove, Maxime; Theroux, Susanna; van de Kamp, Jodie; Watts, Alison Toward a Global Public Repository of Community Protocols to Encourage Best Practices in Biomolecular Ocean Observing and Research Journal Article Frontiers in Marine Science, 8 , pp. 1488, 2021, ISSN: 2296-7745. @article{samuel_toward_2021, title = {Toward a Global Public Repository of Community Protocols to Encourage Best Practices in Biomolecular Ocean Observing and Research}, author = {Robyn M Samuel and Raissa Meyer and Pier Luigi Buttigieg and Neil Davies and Nicholas W Jeffery and Christopher Meyer and Christina Pavloudi and Kathleen Johnson Pitz and Maxime Sweetlove and Susanna Theroux and Jodie van de Kamp and Alison Watts}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/12/2021-Samuel-FMS-79.pdf https://www.frontiersin.org/article/10.3389/fmars.2021.758694}, doi = {10.3389/fmars.2021.758694}, issn = {2296-7745}, year = {2021}, date = {2021-01-01}, urldate = {2021-12-01}, journal = {Frontiers in Marine Science}, volume = {8}, pages = {1488}, abstract = {Biomolecular ocean observing and research is a rapidly evolving field that uses omics approaches to describe biodiversity at its foundational level, giving insight into the structure and function of marine ecosystems over time and space. It is an especially effective approach for investigating the marine microbiome. To mature marine microbiome research and operations within a global ocean biomolecular observing network (OBON) for the UN Decade of Ocean Science for Sustainable Development and beyond, research groups will need a system to effectively share, discover, and compare “omic” practices and protocols. While numerous informatic tools and standards exist, there is currently no global, publicly-supported platform specifically designed for sharing marine omics [or any omics] protocols across the entire value-chain from initiating a study to the publication and use of its results. Toward that goal, we propose the development of the Minimum Information for an Omic Protocol (MIOP), a community-developed guide of curated, standardized metadata tags and categories that will orient protocols in the value-chain for the facilitated, structured, and user-driven discovery of suitable protocol suites on the Ocean Best Practices System. Users can annotate their protocols with these tags, or use them as search criteria to find appropriate protocols. Implementing such a curated repository is an essential step toward establishing best practices. Sharing protocols and encouraging comparisons through this repository will be the first steps toward designing a decision tree to guide users to community endorsed best practices.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Biomolecular ocean observing and research is a rapidly evolving field that uses omics approaches to describe biodiversity at its foundational level, giving insight into the structure and function of marine ecosystems over time and space. It is an especially effective approach for investigating the marine microbiome. To mature marine microbiome research and operations within a global ocean biomolecular observing network (OBON) for the UN Decade of Ocean Science for Sustainable Development and beyond, research groups will need a system to effectively share, discover, and compare “omic” practices and protocols. While numerous informatic tools and standards exist, there is currently no global, publicly-supported platform specifically designed for sharing marine omics [or any omics] protocols across the entire value-chain from initiating a study to the publication and use of its results. Toward that goal, we propose the development of the Minimum Information for an Omic Protocol (MIOP), a community-developed guide of curated, standardized metadata tags and categories that will orient protocols in the value-chain for the facilitated, structured, and user-driven discovery of suitable protocol suites on the Ocean Best Practices System. Users can annotate their protocols with these tags, or use them as search criteria to find appropriate protocols. Implementing such a curated repository is an essential step toward establishing best practices. Sharing protocols and encouraging comparisons through this repository will be the first steps toward designing a decision tree to guide users to community endorsed best practices. |
Georgopoulou, Dimitra G; Stavrakidis-Zachou, Orestis; Mitrizakis, Nikos; Papandroulakis, Nikos Tracking and Analysis of the Movement Behavior of European Seabass (Dicentrarchus labrax) in Aquaculture Systems Journal Article Frontiers in Animal Science, 2 , pp. 79, 2021, ISSN: 2673-6225. @article{georgopoulou_tracking_2021, title = {Tracking and Analysis of the Movement Behavior of European Seabass (Dicentrarchus labrax) in Aquaculture Systems}, author = {Dimitra G Georgopoulou and Orestis Stavrakidis-Zachou and Nikos Mitrizakis and Nikos Papandroulakis}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/01/2021-Georgopoulou-FrontAS-83.pdf https://www.frontiersin.org/article/10.3389/fanim.2021.754520}, doi = {10.3389/fanim.2021.754520}, issn = {2673-6225}, year = {2021}, date = {2021-01-01}, urldate = {2022-01-12}, journal = {Frontiers in Animal Science}, volume = {2}, pages = {79}, abstract = {Monitoring and understanding fish behavior is crucial for achieving precision in everyday husbandry practices (i.e. for optimizing farm performance), and for improving fish welfare in aquaculture. Various intelligent monitoring and control methods, using mathematical models, acoustic methods and computer vision, have been recently developed for this reason. Here, a tracking algorithm based on computer vision that extracts short trajectories of individual European seabass in both recirculating aquaculture systems and sea cages was developed using videos from network cameras. Using this methodology, parameters such as instantaneous normalized speed, travel direction and preference for the tank surface by European seabass could be quantified. When testing the sensitivity of this algorithm for detecting fish swimming variations under different husbandry scenarios, we found that the algorithm could detect variations in all of the abovementioned parameters and could potentially be a useful tool for monitoring the behavioral state of European seabass.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Monitoring and understanding fish behavior is crucial for achieving precision in everyday husbandry practices (i.e. for optimizing farm performance), and for improving fish welfare in aquaculture. Various intelligent monitoring and control methods, using mathematical models, acoustic methods and computer vision, have been recently developed for this reason. Here, a tracking algorithm based on computer vision that extracts short trajectories of individual European seabass in both recirculating aquaculture systems and sea cages was developed using videos from network cameras. Using this methodology, parameters such as instantaneous normalized speed, travel direction and preference for the tank surface by European seabass could be quantified. When testing the sensitivity of this algorithm for detecting fish swimming variations under different husbandry scenarios, we found that the algorithm could detect variations in all of the abovementioned parameters and could potentially be a useful tool for monitoring the behavioral state of European seabass. |
Maidanou, Maria; Koulouri, Panayota; Karachle, Paraskevi K; Arvanitidis, Christos; Koutsoubas, Drosos; Dounas, Costas Trophic Diversity of a Fish Community Associated with a Caulerpa prolifera (Forsskål) Meadow in a Shallow Semi-Enclosed Embayment Journal Article JMSE, 9 (2), pp. 165, 2021, ISSN: 2077-1312. @article{maidanou_trophic_2021, title = {Trophic Diversity of a Fish Community Associated with a Caulerpa prolifera (Forsskål) Meadow in a Shallow Semi-Enclosed Embayment}, author = {Maria Maidanou and Panayota Koulouri and Paraskevi K Karachle and Christos Arvanitidis and Drosos Koutsoubas and Costas Dounas}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/02/2021-Maidanou-JMSE-12.pdf https://www.mdpi.com/2077-1312/9/2/165}, doi = {10.3390/jmse9020165}, issn = {2077-1312}, year = {2021}, date = {2021-01-01}, urldate = {2021-02-24}, journal = {JMSE}, volume = {9}, number = {2}, pages = {165}, abstract = {This study investigates the trophic diversity of fishes living in a meadow of Caulerpa prolifera on a bimonthly basis between May 2006 and April 2007 in a semi-enclosed coastal marine ecosystem of the Mediterranean Sea (Elounda Bay, Crete Island). The study area is shallow and protected from waves, and it is covered by a C. prolifera bed, characterized by high organic input and a highly diverse macrobenthic community. Feeding patterns of the fish, investigated on the basis of stomach content analyses, were described in terms of numerical abundance and frequency of occurrence of prey taxa. A total of 1642 fish individuals, belonging to 17 species, were examined. In total, 45,674 prey individuals were identified belonging to 110 prey taxa, most of which were Malacostraca including their larvae and Copepoda (41,175 individuals identified to 71 taxa). Four different trophic groups were identified: herbivorous, pelagic, benthic (hyperbenthic) and piscivorous. Trophic diversity patterns of the fish species studied were also compared to the relative availability of macrobenthic and zooplanktonic taxa during the same period in the study area. The coexistence of many different, mostly benthic but also pelagic, fishes and their juveniles implies their high trophic flexibility, which is probably important for their survival in this particular habitat. Results of the present study provide basic knowledge on trophic diversity and interactions in the marine ecosystem and, therefore, some evidence as to the protection value of this particular habitat, which is essential for the implementation of a multispecies approach to decision-makers and managers of fisheries sources of the region.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study investigates the trophic diversity of fishes living in a meadow of Caulerpa prolifera on a bimonthly basis between May 2006 and April 2007 in a semi-enclosed coastal marine ecosystem of the Mediterranean Sea (Elounda Bay, Crete Island). The study area is shallow and protected from waves, and it is covered by a C. prolifera bed, characterized by high organic input and a highly diverse macrobenthic community. Feeding patterns of the fish, investigated on the basis of stomach content analyses, were described in terms of numerical abundance and frequency of occurrence of prey taxa. A total of 1642 fish individuals, belonging to 17 species, were examined. In total, 45,674 prey individuals were identified belonging to 110 prey taxa, most of which were Malacostraca including their larvae and Copepoda (41,175 individuals identified to 71 taxa). Four different trophic groups were identified: herbivorous, pelagic, benthic (hyperbenthic) and piscivorous. Trophic diversity patterns of the fish species studied were also compared to the relative availability of macrobenthic and zooplanktonic taxa during the same period in the study area. The coexistence of many different, mostly benthic but also pelagic, fishes and their juveniles implies their high trophic flexibility, which is probably important for their survival in this particular habitat. Results of the present study provide basic knowledge on trophic diversity and interactions in the marine ecosystem and, therefore, some evidence as to the protection value of this particular habitat, which is essential for the implementation of a multispecies approach to decision-makers and managers of fisheries sources of the region. |
Kounna, Christiana; Fountoulaki, Eleni; Miliou, Helen; Chatzifotis, Stavros Aquaculture, 540 , pp. 736683, 2021, ISSN: 00448486. @article{kounna_water_2021, title = {Water temperature effects on growth performance, proximate body and tissue composition, morphometric characteristics and gastrointestinal evacuation processes of juvenile meagre, Argyrosomus regius (Asso 1801)}, author = {Christiana Kounna and Eleni Fountoulaki and Helen Miliou and Stavros Chatzifotis}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848621003458}, doi = {10.1016/j.aquaculture.2021.736683}, issn = {00448486}, year = {2021}, date = {2021-01-01}, urldate = {2021-06-24}, journal = {Aquaculture}, volume = {540}, pages = {736683}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2020 |
Wylensek, David; Hitch, Thomas C A; Riedel, Thomas; Afrizal, Afrizal; Kumar, Neeraj; Wortmann, Esther; Liu, Tianzhe; Devendran, Saravanan; Lesker, Till R; Hernández, Sara B; Heine, Viktoria; Buhl, Eva M; D’Agostino, Paul M; Cumbo, Fabio; Fischöder, Thomas; Wyschkon, Marzena; Looft, Torey; Parreira, Valeria R; Abt, Birte; Doden, Heidi L; Ly, Lindsey; Alves, João M P; Reichlin, Markus; Flisikowski, Krzysztof; Suarez, Laura Navarro; Neumann, Anthony P; Suen, Garret; de Wouters, Tomas; Rohn, Sascha; Lagkouvardos, Ilias; Allen-Vercoe, Emma; Spröer, Cathrin; Bunk, Boyke; Taverne-Thiele, Anja J; Giesbers, Marcel; Wells, Jerry M; Neuhaus, Klaus; Schnieke, Angelika; Cava, Felipe; Segata, Nicola; Elling, Lothar; Strowig, Till; Ridlon, Jason M; Gulder, Tobias A M; Overmann, Jörg; Clavel, Thomas A collection of bacterial isolates from the pig intestine reveals functional and taxonomic diversity Journal Article Nature Communications, 11 (1), pp. 6389, 2020, ISSN: 2041-1723. @article{wylensek_collection_2020, title = {A collection of bacterial isolates from the pig intestine reveals functional and taxonomic diversity}, author = {David Wylensek and Thomas C A Hitch and Thomas Riedel and Afrizal Afrizal and Neeraj Kumar and Esther Wortmann and Tianzhe Liu and Saravanan Devendran and Till R Lesker and Sara B Hernández and Viktoria Heine and Eva M Buhl and Paul M. D’Agostino and Fabio Cumbo and Thomas Fischöder and Marzena Wyschkon and Torey Looft and Valeria R Parreira and Birte Abt and Heidi L Doden and Lindsey Ly and João M P Alves and Markus Reichlin and Krzysztof Flisikowski and Laura Navarro Suarez and Anthony P Neumann and Garret Suen and Tomas de Wouters and Sascha Rohn and Ilias Lagkouvardos and Emma Allen-Vercoe and Cathrin Spröer and Boyke Bunk and Anja J Taverne-Thiele and Marcel Giesbers and Jerry M Wells and Klaus Neuhaus and Angelika Schnieke and Felipe Cava and Nicola Segata and Lothar Elling and Till Strowig and Jason M Ridlon and Tobias A M Gulder and Jörg Overmann and Thomas Clavel}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/07/2020-Wylensek-Nature-Comm.pdf http://www.nature.com/articles/s41467-020-19929-w}, doi = {10.1038/s41467-020-19929-w}, issn = {2041-1723}, year = {2020}, date = {2020-12-01}, urldate = {2021-07-26}, journal = {Nature Communications}, volume = {11}, number = {1}, pages = {6389}, abstract = {Abstract Our knowledge about the gut microbiota of pigs is still scarce, despite the importance of these animals for biomedical research and agriculture. Here, we present a collection of cultured bacteria from the pig gut, including 110 species across 40 families and nine phyla. We provide taxonomic descriptions for 22 novel species and 16 genera. Meta-analysis of 16S rRNA amplicon sequence data and metagenome-assembled genomes reveal prevalent and pig-specific species within Lactobacillus , Streptococcus , Clostridium , Desulfovibrio , Enterococcus , Fusobacterium , and several new genera described in this study. Potentially interesting functions discovered in these organisms include a fucosyltransferase encoded in the genome of the novel species Clostridium porci , and prevalent gene clusters for biosynthesis of sactipeptide-like peptides. Many strains deconjugate primary bile acids in in vitro assays, and a Clostridium scindens strain produces secondary bile acids via dehydroxylation. In addition, cells of the novel species Bullifex porci are coccoidal or spherical under the culture conditions tested, in contrast with the usual helical shape of other members of the family Spirochaetaceae . The strain collection, called ‘Pig intestinal bacterial collection’ (PiBAC), is publicly available at www.dsmz.de/pibac and opens new avenues for functional studies of the pig gut microbiota.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Our knowledge about the gut microbiota of pigs is still scarce, despite the importance of these animals for biomedical research and agriculture. Here, we present a collection of cultured bacteria from the pig gut, including 110 species across 40 families and nine phyla. We provide taxonomic descriptions for 22 novel species and 16 genera. Meta-analysis of 16S rRNA amplicon sequence data and metagenome-assembled genomes reveal prevalent and pig-specific species within Lactobacillus , Streptococcus , Clostridium , Desulfovibrio , Enterococcus , Fusobacterium , and several new genera described in this study. Potentially interesting functions discovered in these organisms include a fucosyltransferase encoded in the genome of the novel species Clostridium porci , and prevalent gene clusters for biosynthesis of sactipeptide-like peptides. Many strains deconjugate primary bile acids in in vitro assays, and a Clostridium scindens strain produces secondary bile acids via dehydroxylation. In addition, cells of the novel species Bullifex porci are coccoidal or spherical under the culture conditions tested, in contrast with the usual helical shape of other members of the family Spirochaetaceae . The strain collection, called ‘Pig intestinal bacterial collection’ (PiBAC), is publicly available at www.dsmz.de/pibac and opens new avenues for functional studies of the pig gut microbiota. |
Rousou, Maria; Plaiti, Wanda; Lowry, Jim; Charalambous, Stephanos; Chintiroglou, Chariton Charles Zootaxa, 4896 (3), pp. 373–408, 2020, ISSN: 1175-5334, 1175-5326, (BIODIV). @article{rousou_amphipoda_2020, title = {Amphipoda species (Suborders: Amphilochidea and Senticaudata) from Vasiliko Bay, Cyprus: New records, information on their biogeography and an annotated checklist from the coasts of Cyprus}, author = {Maria Rousou and Wanda Plaiti and Jim Lowry and Stephanos Charalambous and Chariton Charles Chintiroglou}, url = {https://www.biotaxa.org/Zootaxa/article/view/zootaxa.4896.3.3}, doi = {10.11646/zootaxa.4896.3.3}, issn = {1175-5334, 1175-5326}, year = {2020}, date = {2020-12-01}, urldate = {2022-01-17}, journal = {Zootaxa}, volume = {4896}, number = {3}, pages = {373--408}, abstract = {Current information on soft-bottom benthic amphipod species of the south coasts of Cyprus is scarce. In the summer of 2013, a research survey was carried out, targeting the Vasiliko Bay and the nearby coastal area which is influenced by multiple human-induced stressors. Analysis of 126 samples revealed the presence of 2,122 individual amphipods (Amphilochidea and Senticaudata) belonging to 25 families and 52 species. Twenty-four species are new records for Cyprus increasing the number of amphipods to 141 species. In Vasiliko Bay, two amphipod species presented the highest abundances and were found to be constant (Microdeutopus periergos, Perioculodes longimanus), while seven are common species (Ampelisca brevicornis, Ampelisca typica, Apherusa chiereghinii, Dexamine spinosa, Harpinia crenulata, Leptocheirus pectinatus, Leucothoe incisa) and the remaining 43 species are rare. The highest amphipod total abundances and number of species were recorded in stations with dense Posidonia oceanica meadows and aquaculture units in the vicinity. Brief distributional and ecological characteristics of the identified amphipod species are given and an annotated checklist for the coasts of Cyprus is provided.}, note = {BIODIV}, keywords = {}, pubstate = {published}, tppubtype = {article} } Current information on soft-bottom benthic amphipod species of the south coasts of Cyprus is scarce. In the summer of 2013, a research survey was carried out, targeting the Vasiliko Bay and the nearby coastal area which is influenced by multiple human-induced stressors. Analysis of 126 samples revealed the presence of 2,122 individual amphipods (Amphilochidea and Senticaudata) belonging to 25 families and 52 species. Twenty-four species are new records for Cyprus increasing the number of amphipods to 141 species. In Vasiliko Bay, two amphipod species presented the highest abundances and were found to be constant (Microdeutopus periergos, Perioculodes longimanus), while seven are common species (Ampelisca brevicornis, Ampelisca typica, Apherusa chiereghinii, Dexamine spinosa, Harpinia crenulata, Leptocheirus pectinatus, Leucothoe incisa) and the remaining 43 species are rare. The highest amphipod total abundances and number of species were recorded in stations with dense Posidonia oceanica meadows and aquaculture units in the vicinity. Brief distributional and ecological characteristics of the identified amphipod species are given and an annotated checklist for the coasts of Cyprus is provided. |
Kotzamanis, Yannis; Tsironi, Theofania; Brezas, Andreas; Grigorakis, Kriton; Ilia, Vassiliki; Vatsos, Ioannis; Romano, Nicholas; van Eys, Jan; Kumar, Vikas Scientific Reports, 10 (1), pp. 12294, 2020, ISSN: 2045-2322. @article{kotzamanis_high_2020, title = {High taurine supplementation in plant protein-based diets improves growth and organoleptic characteristics of European seabass (Dicentrarchus labrax)}, author = {Yannis Kotzamanis and Theofania Tsironi and Andreas Brezas and Kriton Grigorakis and Vassiliki Ilia and Ioannis Vatsos and Nicholas Romano and Jan van Eys and Vikas Kumar}, url = {http://www.nature.com/articles/s41598-020-69014-x}, doi = {10.1038/s41598-020-69014-x}, issn = {2045-2322}, year = {2020}, date = {2020-12-01}, urldate = {2020-08-10}, journal = {Scientific Reports}, volume = {10}, number = {1}, pages = {12294}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Zenetos, Argyro; Karachle, Paraskevi K; Corsini-Foka, Maria; Gerovasileiou, Vasilis; Simboura, Nomiki; Xentidis, Nikolas Jason; Tsiamis, Konstantinos Mediterranean Marine Science, 21 (3), pp. 775, 2020, ISSN: 1791-6763, 1108-393X. @article{zenetos_is_2020, title = {Is the trend in new introductions of marine non-indigenous species a reliable criterion for assessing good environmental status? Τhe case study of Greece}, author = {Argyro Zenetos and Paraskevi K Karachle and Maria Corsini-Foka and Vasilis Gerovasileiou and Nomiki Simboura and Nikolas Jason Xentidis and Konstantinos Tsiamis}, url = {https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/25136}, doi = {10.12681/mms.25136}, issn = {1791-6763, 1108-393X}, year = {2020}, date = {2020-12-01}, urldate = {2020-12-08}, journal = {Mediterranean Marine Science}, volume = {21}, number = {3}, pages = {775}, abstract = {This study presents the updated status of marine non-indigenous species (NIS) distribution in Greece and investigates trends in new NIS introductions, at both national and subnational level, during 1970-2017. The overall picture shows an increase in new introductions from the 1970s to 2017. The number of unaided introduced species (mainly Lessepsian immigrants) followed an upward trend in the South Aegean Sea until 2017. Similarly, the number of NIS associated with transport-stowaway (NIS introduced mainly via ballast and boat hulls) followed an upward until 2017 in the South Aegean Sea, but also in the Hellenic Levantine coasts. However, these results are greatly affected by a monitoring bias, which appears to be the main factor influencing the number of new NIS introductions reported from Greece and its subnational areas. This monitoring bias, as well as the continuous influx of Lessepsian NIS into the Aegean Sea, constitutes a challenge for Greece as regards setting exact boundaries for areas with or without Good Environmental Status (GES), based on Descriptor 2, primary criterion C1 (D2C1), of the Marine Strategy Framework Directive (MSFD). Dedicated monitoring of marine NIS should be established and be constant in space, time and across taxonomic groups. Prioritization should be given to hot-spot areas of new NIS introductions, such as ports, aquaculture units and marine protected areas. This should be a prerequisite for applying the primary criterion D2C1 of the MSFD properly, at both national and subnational level. Finally, as regards the implementation of D2C1 of the MSDF and setting exact threshold values, we highlight the need for subregional and regional coordination in the Mediterranean.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study presents the updated status of marine non-indigenous species (NIS) distribution in Greece and investigates trends in new NIS introductions, at both national and subnational level, during 1970-2017. The overall picture shows an increase in new introductions from the 1970s to 2017. The number of unaided introduced species (mainly Lessepsian immigrants) followed an upward trend in the South Aegean Sea until 2017. Similarly, the number of NIS associated with transport-stowaway (NIS introduced mainly via ballast and boat hulls) followed an upward until 2017 in the South Aegean Sea, but also in the Hellenic Levantine coasts. However, these results are greatly affected by a monitoring bias, which appears to be the main factor influencing the number of new NIS introductions reported from Greece and its subnational areas. This monitoring bias, as well as the continuous influx of Lessepsian NIS into the Aegean Sea, constitutes a challenge for Greece as regards setting exact boundaries for areas with or without Good Environmental Status (GES), based on Descriptor 2, primary criterion C1 (D2C1), of the Marine Strategy Framework Directive (MSFD). Dedicated monitoring of marine NIS should be established and be constant in space, time and across taxonomic groups. Prioritization should be given to hot-spot areas of new NIS introductions, such as ports, aquaculture units and marine protected areas. This should be a prerequisite for applying the primary criterion D2C1 of the MSFD properly, at both national and subnational level. Finally, as regards the implementation of D2C1 of the MSDF and setting exact threshold values, we highlight the need for subregional and regional coordination in the Mediterranean. |
Karagiannidis, Efstratios; Konstantinidis, Nikolaos V; Sofidis, Georgios; Chatzinikolaou, Evangelia; Sianos, Georgios BMC Cardiovascular Disorders, 20 (1), pp. 125, 2020, ISSN: 1471-2261. @article{karagiannidis_rationale_2020, title = {Rationale and design of a prospective, observational study for the QUantitative EStimation of Thrombus burden in patients with ST-Elevation Myocardial Infarction using micro-computed tomography: the QUEST-STEMI trial}, author = {Efstratios Karagiannidis and Nikolaos V Konstantinidis and Georgios Sofidis and Evangelia Chatzinikolaou and Georgios Sianos}, url = {https://bmccardiovascdisord.biomedcentral.com/articles/10.1186/s12872-020-01393-5}, doi = {10.1186/s12872-020-01393-5}, issn = {1471-2261}, year = {2020}, date = {2020-12-01}, journal = {BMC Cardiovascular Disorders}, volume = {20}, number = {1}, pages = {125}, abstract = {Although the presence of thrombus in patients with ST-elevation myocardial infarction (STEMI) has been linked to adverse outcomes, routine thrombus aspiration has not been proven effective. A potential explanation is that these patients should be risk-stratified. Traditional clinical, laboratory and angiographic parameters used in clinical trials have been proven inadequate to classify patients. Aspirated thrombotic material characteristics might be an additional important parameter that has not yet been addressed. In this report, we aim to describe a methodological analysis of thrombus aspirated from coronary arteries during primary PCI using micro-Computed $textbackslashTau$omography (micro-CT). These data will be combined with traditional factors to develop a risk-stratification system with high discriminative power for these patients.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Although the presence of thrombus in patients with ST-elevation myocardial infarction (STEMI) has been linked to adverse outcomes, routine thrombus aspiration has not been proven effective. A potential explanation is that these patients should be risk-stratified. Traditional clinical, laboratory and angiographic parameters used in clinical trials have been proven inadequate to classify patients. Aspirated thrombotic material characteristics might be an additional important parameter that has not yet been addressed. In this report, we aim to describe a methodological analysis of thrombus aspirated from coronary arteries during primary PCI using micro-Computed $textbackslashTau$omography (micro-CT). These data will be combined with traditional factors to develop a risk-stratification system with high discriminative power for these patients. |
Mastoraki, Maria; Ferrándiz, Paula Mollá; Vardali, Sofia C; Kontodimas, Demetrius C; Kotzamanis, Yannis P; Gasco, Laura; Chatzifotis, Stavros; Antonopoulou, Efthimia Aquaculture, 528 , pp. 735511, 2020, ISSN: 00448486. @article{mastoraki_comparative_2020, title = {A comparative study on the effect of fish meal substitution with three different insect meals on growth, body composition and metabolism of European sea bass (Dicentrarchus labrax L.)}, author = {Maria Mastoraki and Paula Mollá Ferrándiz and Sofia C Vardali and Demetrius C Kontodimas and Yannis P Kotzamanis and Laura Gasco and Stavros Chatzifotis and Efthimia Antonopoulou}, url = {https://linkinghub.elsevier.com/retrieve/pii/S004484862030065X}, doi = {10.1016/j.aquaculture.2020.735511}, issn = {00448486}, year = {2020}, date = {2020-11-01}, urldate = {2020-07-06}, journal = {Aquaculture}, volume = {528}, pages = {735511}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Obst, Matthias; Exter, Katrina; Allcock, Louise A; Arvanitidis, Christos; Axberg, Alizz; Bustamante, Maria; Cancio, Ibon; Carreira-Flores, Diego; Chatzinikolaou, Eva; Chatzigeorgiou, Giorgos; Chrismas, Nathan; Clark, Melody S; Comtet, Thierry; Dailianis, Thanos; Davies, Neil; Deneudt, Klaas; de Cerio, Oihane Diaz; Fortič, Ana; Gerovasileiou, Vasilis; Hablützel, Pascal I; Keklikoglou, Kleoniki; Kotoulas, Georgios; Lasota, Rafal; Leite, Barbara R; Loisel, Stéphane; Lévêque, Laurent; Levy, Liraz; Malachowicz, Magdalena; Mavrič, Borut; Meyer, Christopher; Mortelmans, Jonas; Norkko, Joanna; Pade, Nicolas; Power, Anne Marie; Ramšak, Andreja; Reiss, Henning; Solbakken, Jostein; Staehr, Peter A; Sundberg, Per; Thyrring, Jakob; Troncoso, Jesus S; Viard, Frédérique; Wenne, Roman; Yperifanou, Eleni Ioanna; Zbawicka, Malgorzata; Pavloudi, Christina A Marine Biodiversity Observation Network for Genetic Monitoring of Hard-Bottom Communities (ARMS-MBON) Journal Article Frontiers in Marine Science, 7 , pp. 572680, 2020, ISSN: 2296-7745. @article{obst_marine_2020, title = {A Marine Biodiversity Observation Network for Genetic Monitoring of Hard-Bottom Communities (ARMS-MBON)}, author = {Matthias Obst and Katrina Exter and Louise A Allcock and Christos Arvanitidis and Alizz Axberg and Maria Bustamante and Ibon Cancio and Diego Carreira-Flores and Eva Chatzinikolaou and Giorgos Chatzigeorgiou and Nathan Chrismas and Melody S Clark and Thierry Comtet and Thanos Dailianis and Neil Davies and Klaas Deneudt and Oihane Diaz de Cerio and Ana Fortič and Vasilis Gerovasileiou and Pascal I Hablützel and Kleoniki Keklikoglou and Georgios Kotoulas and Rafal Lasota and Barbara R Leite and Stéphane Loisel and Laurent Lévêque and Liraz Levy and Magdalena Malachowicz and Borut Mavrič and Christopher Meyer and Jonas Mortelmans and Joanna Norkko and Nicolas Pade and Anne Marie Power and Andreja Ramšak and Henning Reiss and Jostein Solbakken and Peter A Staehr and Per Sundberg and Jakob Thyrring and Jesus S Troncoso and Frédérique Viard and Roman Wenne and Eleni Ioanna Yperifanou and Malgorzata Zbawicka and Christina Pavloudi}, url = {https://www.frontiersin.org/articles/10.3389/fmars.2020.572680/full}, doi = {10.3389/fmars.2020.572680}, issn = {2296-7745}, year = {2020}, date = {2020-11-01}, urldate = {2020-12-08}, journal = {Frontiers in Marine Science}, volume = {7}, pages = {572680}, abstract = {Marine hard-bottom communities are undergoing severe change under the influence of multiple drivers, notably climate change, extraction of natural resources, pollution and eutrophication, habitat degradation, and invasive species. Monitoring marine biodiversity in such habitats is, however, challenging as it typically involves expensive, non-standardized, and often destructive sampling methods that limit its scalability. Differences in monitoring approaches furthermore hinders inter-comparison among monitoring programs. Here, we announce a Marine Biodiversity Observation Network (MBON) consisting of Autonomous Reef Monitoring Structures (ARMS) with the aim to assess the status and changes in benthic fauna with genomic-based methods, notably DNA metabarcoding, in combination with image-based identifications. This article presents the results of a 30-month pilot phase in which we established an operational and geographically expansive ARMS-MBON. The network currently consists of 20 observatories distributed across European coastal waters and the polar regions, in which 134 ARMS have been deployed to date. Sampling takes place annually, either as short-term deployments during the summer or as long-term deployments starting in spring. The pilot phase was used to establish a common set of standards for field sampling, genetic analysis, data management, and legal compliance, which are presented here. We also tested the potential of ARMS for combining genetic and image-based identification methods in comparative studies of benthic diversity, as well as for detecting non-indigenous species. Results show that ARMS are suitable for monitoring hard-bottom environments as they provide genetic data that can be continuously enriched, re-analyzed, and integrated with conventional data to document benthic community composition and detect non-indigenous species. Finally, we provide guidelines to expand the network and present a sustainability plan as part of the European Marine Biological Resource Centre ( www.embrc.eu ).}, keywords = {}, pubstate = {published}, tppubtype = {article} } Marine hard-bottom communities are undergoing severe change under the influence of multiple drivers, notably climate change, extraction of natural resources, pollution and eutrophication, habitat degradation, and invasive species. Monitoring marine biodiversity in such habitats is, however, challenging as it typically involves expensive, non-standardized, and often destructive sampling methods that limit its scalability. Differences in monitoring approaches furthermore hinders inter-comparison among monitoring programs. Here, we announce a Marine Biodiversity Observation Network (MBON) consisting of Autonomous Reef Monitoring Structures (ARMS) with the aim to assess the status and changes in benthic fauna with genomic-based methods, notably DNA metabarcoding, in combination with image-based identifications. This article presents the results of a 30-month pilot phase in which we established an operational and geographically expansive ARMS-MBON. The network currently consists of 20 observatories distributed across European coastal waters and the polar regions, in which 134 ARMS have been deployed to date. Sampling takes place annually, either as short-term deployments during the summer or as long-term deployments starting in spring. The pilot phase was used to establish a common set of standards for field sampling, genetic analysis, data management, and legal compliance, which are presented here. We also tested the potential of ARMS for combining genetic and image-based identification methods in comparative studies of benthic diversity, as well as for detecting non-indigenous species. Results show that ARMS are suitable for monitoring hard-bottom environments as they provide genetic data that can be continuously enriched, re-analyzed, and integrated with conventional data to document benthic community composition and detect non-indigenous species. Finally, we provide guidelines to expand the network and present a sustainability plan as part of the European Marine Biological Resource Centre ( www.embrc.eu ). |
Emmanouilidis, Alexandros; Unkel, Ingmar; Seguin, Joana; Keklikoglou, Kleoniki; Gianni, Eleni; Avramidis, Pavlos Application of Non-Destructive Techniques on a Varve Sediment Record from Vouliagmeni Coastal Lake, Eastern Gulf of Corinth, Greece Journal Article Applied Sciences, 10 (22), pp. 8273, 2020, ISSN: 2076-3417. @article{emmanouilidis_application_2020, title = {Application of Non-Destructive Techniques on a Varve Sediment Record from Vouliagmeni Coastal Lake, Eastern Gulf of Corinth, Greece}, author = {Alexandros Emmanouilidis and Ingmar Unkel and Joana Seguin and Kleoniki Keklikoglou and Eleni Gianni and Pavlos Avramidis}, url = {https://www.mdpi.com/2076-3417/10/22/8273}, doi = {10.3390/app10228273}, issn = {2076-3417}, year = {2020}, date = {2020-11-01}, urldate = {2020-11-23}, journal = {Applied Sciences}, volume = {10}, number = {22}, pages = {8273}, abstract = {During the last few decades, X-ray attenuation systems have been established as standard techniques in geosciences and as excellent scientific tools for the analysis of sedimentary facies and structures. In the present study, we use two non-destructive and high-resolution systems (computed tomography, X-ray fluorescence) to address all sedimentological facies and structural characteristics on a 6 m long, partly laminated sediment record, from Vouliagmeni lake, located at the eastern part of the Corinth Gulf, Greece. Vouliagmeni lake is the deepest coastal lake in Greece, and its location is of great importance since it is located in one of the most tectonically active regions in the world. The chronological framework of the retrieved sediment sequence spans the last 12,000 years, with alternations of laminated and non-laminated sections. The annual accumulation of the laminated sequences was determined through the radionuclide concentration of 14 samples. Scanning tomography was performed with a medical CT scanner and a μCT scanner, aiming to compare the potentials and variations of both methods. Lamination boundaries, event layers (turbidites) and sediment deformations were distinguished through processing the extracted 3D rendered volumes, after applying ranges depending on Hounsfield (HU) values. Microscopic analysis revealed three main layer types in the varve sequences that were examined, attributing to summer/spring, autumn and winter deposits. Statistical analysis of the elemental composition, magnetic susceptibility (MS) measurements and HU values revealed three clusters/facies, reflecting climatic and in-lake hydrological changes. Cluster A emulates sedimentation during arid conditions, while Cluster B reflects humid conditions with increased precipitation and erosion. Cluster C represents sequences with homogenous Ca-rich sediment. Our proposed workflow highlights the possible correlation between the non-destructive variables that were measured, but also the variations and applications of each method and software used during this study.}, keywords = {}, pubstate = {published}, tppubtype = {article} } During the last few decades, X-ray attenuation systems have been established as standard techniques in geosciences and as excellent scientific tools for the analysis of sedimentary facies and structures. In the present study, we use two non-destructive and high-resolution systems (computed tomography, X-ray fluorescence) to address all sedimentological facies and structural characteristics on a 6 m long, partly laminated sediment record, from Vouliagmeni lake, located at the eastern part of the Corinth Gulf, Greece. Vouliagmeni lake is the deepest coastal lake in Greece, and its location is of great importance since it is located in one of the most tectonically active regions in the world. The chronological framework of the retrieved sediment sequence spans the last 12,000 years, with alternations of laminated and non-laminated sections. The annual accumulation of the laminated sequences was determined through the radionuclide concentration of 14 samples. Scanning tomography was performed with a medical CT scanner and a μCT scanner, aiming to compare the potentials and variations of both methods. Lamination boundaries, event layers (turbidites) and sediment deformations were distinguished through processing the extracted 3D rendered volumes, after applying ranges depending on Hounsfield (HU) values. Microscopic analysis revealed three main layer types in the varve sequences that were examined, attributing to summer/spring, autumn and winter deposits. Statistical analysis of the elemental composition, magnetic susceptibility (MS) measurements and HU values revealed three clusters/facies, reflecting climatic and in-lake hydrological changes. Cluster A emulates sedimentation during arid conditions, while Cluster B reflects humid conditions with increased precipitation and erosion. Cluster C represents sequences with homogenous Ca-rich sediment. Our proposed workflow highlights the possible correlation between the non-destructive variables that were measured, but also the variations and applications of each method and software used during this study. |
Costa, Leonardo Lopes; Zalmon, Ilana Rosental; Fanini, Lucia; Defeo, Omar Macroinvertebrates as indicators of human disturbances on sandy beaches: A global review Journal Article Ecological Indicators, 118 , pp. 106764, 2020, ISSN: 1470160X. @article{costa_macroinvertebrates_2020, title = {Macroinvertebrates as indicators of human disturbances on sandy beaches: A global review}, author = {Leonardo Lopes Costa and Ilana Rosental Zalmon and Lucia Fanini and Omar Defeo}, url = {https://linkinghub.elsevier.com/retrieve/pii/S1470160X20307020}, doi = {10.1016/j.ecolind.2020.106764}, issn = {1470160X}, year = {2020}, date = {2020-11-01}, urldate = {2020-09-17}, journal = {Ecological Indicators}, volume = {118}, pages = {106764}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Kogiannou, Dimitra; Rigos, George Praziquantel depletion from muscle plus skin tissue of gilthead sea bream (Sparus aurata) Journal Article Mediterranean Marine Science, 22 (1), pp. 121–124, 2020, ISSN: 1791-6763, 1108-393X, (AQUA). @article{kogiannou_praziquantel_2020, title = {Praziquantel depletion from muscle plus skin tissue of gilthead sea bream (Sparus aurata)}, author = {Dimitra Kogiannou and George Rigos}, url = {https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/25126}, doi = {10.12681/mms.25126}, issn = {1791-6763, 1108-393X}, year = {2020}, date = {2020-11-01}, urldate = {2022-01-17}, journal = {Mediterranean Marine Science}, volume = {22}, number = {1}, pages = {121--124}, abstract = {The depletion pattern of praziquantel (PZQ) from gilthead sea bream (Sparus aurata) muscle plus skin was determined in this study. Fish averaging 100.2 ± 17.7 g and kept at 25oC received a PZQ-dosing of 150 mg/kg fish for 3 days. Muscle plus skin of each of ten fish were collected on days 1, 2, 3, 4, and 6 days post-treatment. Depletion of PZQ from the edible gilthead sea bream tissues was rapid, as its concentrations declined to 0.04 μg/g as early as 24 h post-treatment, while was undetectable at 72 h. This information confirms the fact that PZQ is depleted fast from farmed animals including fish such as the gilthead sea bream and its levels in edible tissues failed below the detection limit in approximately 75 dd.}, note = {AQUA}, keywords = {}, pubstate = {published}, tppubtype = {article} } The depletion pattern of praziquantel (PZQ) from gilthead sea bream (Sparus aurata) muscle plus skin was determined in this study. Fish averaging 100.2 ± 17.7 g and kept at 25oC received a PZQ-dosing of 150 mg/kg fish for 3 days. Muscle plus skin of each of ten fish were collected on days 1, 2, 3, 4, and 6 days post-treatment. Depletion of PZQ from the edible gilthead sea bream tissues was rapid, as its concentrations declined to 0.04 μg/g as early as 24 h post-treatment, while was undetectable at 72 h. This information confirms the fact that PZQ is depleted fast from farmed animals including fish such as the gilthead sea bream and its levels in edible tissues failed below the detection limit in approximately 75 dd. |
Rosso, Antonietta; Gerovasileiou, Vasilis; Martino, Emanuela Di Really Onychocellids? Revisions and New Findings Increase the Astonishing Bryozoan Diversity of the Mediterranean Sea Journal Article Journal of Marine Science and Engineering, 8 (11), pp. 904, 2020, ISSN: 2077-1312. @article{rosso_really_2020, title = {Really Onychocellids? Revisions and New Findings Increase the Astonishing Bryozoan Diversity of the Mediterranean Sea}, author = {Antonietta Rosso and Vasilis Gerovasileiou and Emanuela Di Martino}, url = {https://www.mdpi.com/2077-1312/8/11/904}, doi = {10.3390/jmse8110904}, issn = {2077-1312}, year = {2020}, date = {2020-11-01}, urldate = {2020-12-08}, journal = {Journal of Marine Science and Engineering}, volume = {8}, number = {11}, pages = {904}, abstract = {Investigation of bryozoan faunas collected in two submarine caves in Lesvos Island, Aegean Sea revealed a great number of colonies of three species currently assigned to the cheilostome family Onychocellidae: Onychocella marioni Jullien, 1882, O. vibraculifera Neviani, 1895, and Smittipora disjuncta Canu & Bassler, 1930. All species were first described and subsequently recorded on several occasions, from the Mediterranean Sea, particularly from the Aegean Sea. The availability of this material provided the basis for more detailed observations and first scanning electron microscopy (SEM) study of some diagnostic characters, including ovicells and ancestrulae, for the well-known species, as well as a few colonies of a species left in open nomenclature (i.e., Onychocellidae sp. 1) in previous works. In this paper we (i) update the descriptions of these four species; (ii) resurrect the species Floridinella arculifera Canu & Bassler, 1927, which was previously synonymised with Caleschara minuta (Maplestone, 1909), suggesting for it the new combination Tretosina arculifera; (iii) and introduce the new genus Bryobifallax for S. disjuncta.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Investigation of bryozoan faunas collected in two submarine caves in Lesvos Island, Aegean Sea revealed a great number of colonies of three species currently assigned to the cheilostome family Onychocellidae: Onychocella marioni Jullien, 1882, O. vibraculifera Neviani, 1895, and Smittipora disjuncta Canu & Bassler, 1930. All species were first described and subsequently recorded on several occasions, from the Mediterranean Sea, particularly from the Aegean Sea. The availability of this material provided the basis for more detailed observations and first scanning electron microscopy (SEM) study of some diagnostic characters, including ovicells and ancestrulae, for the well-known species, as well as a few colonies of a species left in open nomenclature (i.e., Onychocellidae sp. 1) in previous works. In this paper we (i) update the descriptions of these four species; (ii) resurrect the species Floridinella arculifera Canu & Bassler, 1927, which was previously synonymised with Caleschara minuta (Maplestone, 1909), suggesting for it the new combination Tretosina arculifera; (iii) and introduce the new genus Bryobifallax for S. disjuncta. |
Bolgan, Marta; Pereira, Beatriz P; Crucianelli, Aurora; Mylonas, Constantinos C; Pousão-Ferreira, Pedro; Parmentier, Eric; Fonseca, Paulo J; Amorim, Clara M P Vocal repertoire and consistency of call features in the meagre Argyrosomous regius (Asso, 1801) Journal Article PLOS ONE, 15 (11), pp. e0241792, 2020, ISSN: 1932-6203. @article{bolgan_vocal_2020, title = {Vocal repertoire and consistency of call features in the meagre Argyrosomous regius (Asso, 1801)}, author = {Marta Bolgan and Beatriz P Pereira and Aurora Crucianelli and Constantinos C Mylonas and Pedro Pousão-Ferreira and Eric Parmentier and Paulo J Fonseca and Clara M P Amorim}, editor = {Dennis M Higgs}, url = {https://dx.plos.org/10.1371/journal.pone.0241792}, doi = {10.1371/journal.pone.0241792}, issn = {1932-6203}, year = {2020}, date = {2020-11-01}, urldate = {2020-12-08}, journal = {PLOS ONE}, volume = {15}, number = {11}, pages = {e0241792}, abstract = {Passive Acoustic Monitoring (PAM) is a non-intrusive and cost-effective method capable of providing high-resolution, long-term information on the status and health of vocal populations and communities. To successfully monitor the same species over wide geographical and temporal scales, it is necessary to characterise the range of sound variability, as well as the consistency of sound features between populations. The meagre ( Argyrosomus regius , Asso 1801) is an interesting case study because recent investigations suggest a wider vocal repertoire than previously described. In this study, meagre vocalizations were recorded and analysed from a variety of settings, ranging from rearing facilities to wild populations to provide a comprehensive characterisation of its vocal repertoire, while investigating the consistency of spawning sound features between populations. All sounds presented a similar acoustic structure in their basic unit ( i . e . the pulse), while an important variability was found in the number of pulses; the meagre can emit sounds made of one single pulse or many pulses (up to more than 100). High level of overlap in the Principal Component Analysis made difficult to differentiate sound type clusters. Despite this, two sound types were identifiable: knocks (sounds from 1 to 3 pulses) and long grunts (sounds with more than 29 pulses). Discriminant Analysis carried out on PCA residuals showed that knock had the highest proportion of correct placement (92% of the observations correctly placed) followed by long grunts (80%). All other previously described sound types (intermediate grunt, short grunt and disturbance sounds) could not be separated and presented low levels of correct placement, suggesting that care should be taken when defining these as independent sound types. Finally, acoustic features consistency was found in meagre grunts emitted by different populations during spawning nights; statistical differences could be explained by recording settings and fish conditions. The results of this study provide important information for fostering PAM programs of wild meagre populations, while contributing to the discussion around the definition of fish sound types in vocal fish communities. Studies of this kind, which evaluate both variability and consistency of sound features, are of fundamental importance for maximising PAM efforts in the wild, at both the specific and the community level.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Passive Acoustic Monitoring (PAM) is a non-intrusive and cost-effective method capable of providing high-resolution, long-term information on the status and health of vocal populations and communities. To successfully monitor the same species over wide geographical and temporal scales, it is necessary to characterise the range of sound variability, as well as the consistency of sound features between populations. The meagre ( Argyrosomus regius , Asso 1801) is an interesting case study because recent investigations suggest a wider vocal repertoire than previously described. In this study, meagre vocalizations were recorded and analysed from a variety of settings, ranging from rearing facilities to wild populations to provide a comprehensive characterisation of its vocal repertoire, while investigating the consistency of spawning sound features between populations. All sounds presented a similar acoustic structure in their basic unit ( i . e . the pulse), while an important variability was found in the number of pulses; the meagre can emit sounds made of one single pulse or many pulses (up to more than 100). High level of overlap in the Principal Component Analysis made difficult to differentiate sound type clusters. Despite this, two sound types were identifiable: knocks (sounds from 1 to 3 pulses) and long grunts (sounds with more than 29 pulses). Discriminant Analysis carried out on PCA residuals showed that knock had the highest proportion of correct placement (92% of the observations correctly placed) followed by long grunts (80%). All other previously described sound types (intermediate grunt, short grunt and disturbance sounds) could not be separated and presented low levels of correct placement, suggesting that care should be taken when defining these as independent sound types. Finally, acoustic features consistency was found in meagre grunts emitted by different populations during spawning nights; statistical differences could be explained by recording settings and fish conditions. The results of this study provide important information for fostering PAM programs of wild meagre populations, while contributing to the discussion around the definition of fish sound types in vocal fish communities. Studies of this kind, which evaluate both variability and consistency of sound features, are of fundamental importance for maximising PAM efforts in the wild, at both the specific and the community level. |
Misol, Gerald N; Kokkari, Constantinta; Katharios, Pantelis Complete Genome Sequence of a Jumbo Bacteriophage, vB_pir03, against textitVibrio harveyi Journal Article Microbiology Resource Announcements, 9 (44), pp. e00910–20, /mra/9/44/MRA.00910–20.atom, 2020, ISSN: 2576-098X. @article{misol_complete_2020, title = {Complete Genome Sequence of a Jumbo Bacteriophage, vB_pir03, against textitVibrio harveyi}, author = {Gerald N Misol and Constantinta Kokkari and Pantelis Katharios}, editor = {John J Dennehy}, url = {https://mra.asm.org/content/9/44/e00910-20 https://imbbc.hcmr.gr/wp-content/uploads/2020/12/Katharios-2020-Microbiology-Resource-Announcements.pdf}, doi = {10.1128/MRA.00910-20}, issn = {2576-098X}, year = {2020}, date = {2020-10-01}, urldate = {2020-12-08}, journal = {Microbiology Resource Announcements}, volume = {9}, number = {44}, pages = {e00910--20, /mra/9/44/MRA.00910--20.atom}, abstract = {ABSTRACT Vibrio harveyi is a persistent pathogen responsible for disease outbreaks in aquaculture. We have sequenced the genome of a jumbo Vibrio phage, vB_pir03, isolated in Greece. Here, we present the complete genome of vB_pir03, which consists of 286,284 bp and 336 open reading frames.}, keywords = {}, pubstate = {published}, tppubtype = {article} } ABSTRACT Vibrio harveyi is a persistent pathogen responsible for disease outbreaks in aquaculture. We have sequenced the genome of a jumbo Vibrio phage, vB_pir03, isolated in Greece. Here, we present the complete genome of vB_pir03, which consists of 286,284 bp and 336 open reading frames. |
Dimogianopoulos, D; Grigorakis, K Effective algorithmic operational framework for fish texture evaluation in industry: Achieving maturity Journal Article Aquaculture and Fisheries, pp. S2468550X20301386, 2020, ISSN: 2468550X, (AQUA). @article{dimogianopoulos_effective_2020, title = {Effective algorithmic operational framework for fish texture evaluation in industry: Achieving maturity}, author = {D Dimogianopoulos and K Grigorakis}, url = {https://linkinghub.elsevier.com/retrieve/pii/S2468550X20301386}, doi = {10.1016/j.aaf.2020.10.001}, issn = {2468550X}, year = {2020}, date = {2020-10-01}, urldate = {2022-01-17}, journal = {Aquaculture and Fisheries}, pages = {S2468550X20301386}, note = {AQUA}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Fakriadis, Ioannis; Zanatta, Eugenio Maria; Fleck, Renata Pontes Dos Santos; Mateo, Daybet Lorena Sena; Papadaki, Maria; Mylonas, Constantinos C Endocrine regulation of long-term enhancement of spermiation in meagre (Argyrosomus regius) with GnRHa controlled-delivery systems Journal Article General and Comparative Endocrinology, 297 , pp. 113549, 2020, ISSN: 00166480. @article{fakriadis_endocrine_2020, title = {Endocrine regulation of long-term enhancement of spermiation in meagre (Argyrosomus regius) with GnRHa controlled-delivery systems}, author = {Ioannis Fakriadis and Eugenio Maria Zanatta and Renata Pontes Dos Santos Fleck and Daybet Lorena Sena Mateo and Maria Papadaki and Constantinos C Mylonas}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0016648020303026}, doi = {10.1016/j.ygcen.2020.113549}, issn = {00166480}, year = {2020}, date = {2020-10-01}, urldate = {2020-08-06}, journal = {General and Comparative Endocrinology}, volume = {297}, pages = {113549}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Papadaki, Maria; Kaitetzidou, Elisavet; Mylonas, Constantinos C; Sarropoulou, Elena Non-coding RNA Expression Patterns of Two Different Teleost Gonad Maturation Stages Journal Article Marine Biotechnology, 22 (5), pp. 683–695, 2020, ISSN: 1436-2228, 1436-2236. @article{papadaki_non-coding_2020, title = {Non-coding RNA Expression Patterns of Two Different Teleost Gonad Maturation Stages}, author = {Maria Papadaki and Elisavet Kaitetzidou and Constantinos C Mylonas and Elena Sarropoulou}, url = {http://link.springer.com/10.1007/s10126-020-09991-2 https://imbbc.hcmr.gr/wp-content/uploads/2020/10/Papadaki-2020-MBTE-pre-print.pdf}, doi = {10.1007/s10126-020-09991-2}, issn = {1436-2228, 1436-2236}, year = {2020}, date = {2020-10-01}, urldate = {2020-10-05}, journal = {Marine Biotechnology}, volume = {22}, number = {5}, pages = {683--695}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Chatziantoniou, Andromachi; Bakopoulos, Vasileios; Papandroulakis, Nikos; Topouzelis, Konstantinos Detection of biogenic oil film near aquaculture sites seen by Sentinel-2 multispectral images Inproceedings Bostater, Charles R; Neyt, Xavier; Viallefont-Robinet, Françoise (Ed.): Remote Sensing of the Ocean, Sea Ice, Coastal Waters, and Large Water Regions 2020, pp. 4, SPIE, Online Only, United Kingdom, 2020, ISBN: 978-1-5106-3871-6 978-1-5106-3872-3. @inproceedings{chatziantoniou_detection_2020, title = {Detection of biogenic oil film near aquaculture sites seen by Sentinel-2 multispectral images}, author = {Andromachi Chatziantoniou and Vasileios Bakopoulos and Nikos Papandroulakis and Konstantinos Topouzelis}, editor = {Charles R Bostater and Xavier Neyt and Françoise Viallefont-Robinet}, url = {https://www.spiedigitallibrary.org/conference-proceedings-of-spie/11529/2573455/Detection-of-biogenic-oil-film-near-aquaculture-sites-seen-by/10.1117/12.2573455.full}, doi = {10.1117/12.2573455}, isbn = {978-1-5106-3871-6 978-1-5106-3872-3}, year = {2020}, date = {2020-09-01}, urldate = {2020-10-05}, booktitle = {Remote Sensing of the Ocean, Sea Ice, Coastal Waters, and Large Water Regions 2020}, pages = {4}, publisher = {SPIE}, address = {Online Only, United Kingdom}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Henry, Morgane A; Kokou, Fotini; Palenzuela, Oswaldo; Pyrenis, George; Rigos, George Folia Parasitologica, 67 , 2020, ISSN: 00155683, 18036465. @article{henry_experimental_2020, title = {Experimental infection model with the bivalvulid Enteromyxum leei (Myxidiidae) in the sharpsnout seabream, Diplodus puntazzo (Sparidae), and evaluation of the antiparasitic efficacy of a functional diet}, author = {Morgane A Henry and Fotini Kokou and Oswaldo Palenzuela and George Pyrenis and George Rigos}, url = {http://folia.paru.cas.cz/doi/10.14411/fp.2020.024.html}, doi = {10.14411/fp.2020.024}, issn = {00155683, 18036465}, year = {2020}, date = {2020-09-01}, urldate = {2020-12-08}, journal = {Folia Parasitologica}, volume = {67}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Digenis, Markos; Gerovasileiou, Vasilis First Record of Leucothea multicornis (Quoy & Gaimard, 1824) (Ctenophora: Leucotheidae) in Greek Waters Journal Article Acta Zoologica Bulgarica, 72 (3), pp. 499–500, 2020, (BIODIV). @article{digenis_first_2020, title = {First Record of Leucothea multicornis (Quoy & Gaimard, 1824) (Ctenophora: Leucotheidae) in Greek Waters}, author = {Markos Digenis and Vasilis Gerovasileiou}, url = {http://www.acta-zoologica-bulgarica.eu/002402}, year = {2020}, date = {2020-09-01}, journal = {Acta Zoologica Bulgarica}, volume = {72}, number = {3}, pages = {499--500}, abstract = {The lobate ctenophore Leucothea multicornis (Quoy & Gaimard, 1824) was recorded off Kefalonia Is- land, Greece, in July 2019. This new record, in the Eastern Ionian Sea, fills distribution gaps between the central Mediterranean basin and the Levantine Sea.}, note = {BIODIV}, keywords = {}, pubstate = {published}, tppubtype = {article} } The lobate ctenophore Leucothea multicornis (Quoy & Gaimard, 1824) was recorded off Kefalonia Is- land, Greece, in July 2019. This new record, in the Eastern Ionian Sea, fills distribution gaps between the central Mediterranean basin and the Levantine Sea. |
Polymenakou, Paraskevi N; Mandalakis, Manolis; Macheras, Michalis; Oulas, Anastasis; Kristoffersen, Jon Bent; Christakis, Christos A; Terzoglou, Vasso; Stavroulaki, Melanthia High genetic diversity and variability of microbial communities in near-surface atmosphere of Crete island, Greece Journal Article Aerobiologia, 36 (3), pp. 341–353, 2020, ISSN: 0393-5965, 1573-3025. @article{polymenakou_high_2020, title = {High genetic diversity and variability of microbial communities in near-surface atmosphere of Crete island, Greece}, author = {Paraskevi N Polymenakou and Manolis Mandalakis and Michalis Macheras and Anastasis Oulas and Jon Bent Kristoffersen and Christos A Christakis and Vasso Terzoglou and Melanthia Stavroulaki}, url = {http://link.springer.com/10.1007/s10453-020-09636-w}, doi = {10.1007/s10453-020-09636-w}, issn = {0393-5965, 1573-3025}, year = {2020}, date = {2020-09-01}, urldate = {2020-08-31}, journal = {Aerobiologia}, volume = {36}, number = {3}, pages = {341--353}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Chatziplis, Dimitrios; Oikonomou, Stavroula; Loukovitis, Dimitrios; Tsiokos, Dimitrios; Samaras, Athanasios; Dimitroglou, Arkadios; Kottaras, Lefteris; Papanna, Kantham; Papaharisis, Leonidas; Tsigenopoulos, Costas; Pavlidis, Michail QTL for Stress and Disease Resistance in European Sea Bass, Dicentrarhus labrax L. Journal Article Animals, 10 (9), pp. 1668, 2020, ISSN: 2076-2615. @article{chatziplis_qtl_2020, title = {QTL for Stress and Disease Resistance in European Sea Bass, Dicentrarhus labrax L.}, author = {Dimitrios Chatziplis and Stavroula Oikonomou and Dimitrios Loukovitis and Dimitrios Tsiokos and Athanasios Samaras and Arkadios Dimitroglou and Lefteris Kottaras and Kantham Papanna and Leonidas Papaharisis and Costas Tsigenopoulos and Michail Pavlidis}, url = {https://www.mdpi.com/2076-2615/10/9/1668}, doi = {10.3390/ani10091668}, issn = {2076-2615}, year = {2020}, date = {2020-09-01}, urldate = {2020-10-05}, journal = {Animals}, volume = {10}, number = {9}, pages = {1668}, abstract = {There is a growing interest in selective breeding in European sea bass (Dicentrarchus labrax), especially regarding family selection based on growth performance. In particular, quantitative trait loci (QTL) identification in sea bass enhances the application of marker-assisted breeding for the genetic improvement of the production traits. The aims of the study were to identify potential QTL affecting stress and immunological indicators, body weight, and mortality after vibriosis injection in sea bass as well as to estimate heritability and genetic/phenotypic correlations for the aforementioned traits. To this end, stress test was performed on 960 offspring and a sub-group of them (420) was selected to explore the mortality after vibrio injection. Selective genotyping was performed in 620 offspring for 35 microsatellite markers and distributed into 6 linkage groups. The length of the genetic linkage map was 283.6 cM and the mean distance between the markers was 8.1 cM. QTL affecting body weight in three different growth periods detected on linkage groups LG1, LG4, LG6, and LG14. A QTL associated with weight in early growth stages (290–306 days post-hatching) was also identified on LG3. QTL analysis confirmed the existence of QTL affecting cortisol levels, on LG3 and LG14. Moreover, new QTL affecting only cortisol and glucose levels were detected on LG1 and LG23. No QTL affecting hormonal or biochemical marks was found on LG4 and LG6. Heritability of cortisol, lysozyme levels, and mortality were high (0.36, 0.55, and 0.38, respectively).}, keywords = {}, pubstate = {published}, tppubtype = {article} } There is a growing interest in selective breeding in European sea bass (Dicentrarchus labrax), especially regarding family selection based on growth performance. In particular, quantitative trait loci (QTL) identification in sea bass enhances the application of marker-assisted breeding for the genetic improvement of the production traits. The aims of the study were to identify potential QTL affecting stress and immunological indicators, body weight, and mortality after vibriosis injection in sea bass as well as to estimate heritability and genetic/phenotypic correlations for the aforementioned traits. To this end, stress test was performed on 960 offspring and a sub-group of them (420) was selected to explore the mortality after vibrio injection. Selective genotyping was performed in 620 offspring for 35 microsatellite markers and distributed into 6 linkage groups. The length of the genetic linkage map was 283.6 cM and the mean distance between the markers was 8.1 cM. QTL affecting body weight in three different growth periods detected on linkage groups LG1, LG4, LG6, and LG14. A QTL associated with weight in early growth stages (290–306 days post-hatching) was also identified on LG3. QTL analysis confirmed the existence of QTL affecting cortisol levels, on LG3 and LG14. Moreover, new QTL affecting only cortisol and glucose levels were detected on LG1 and LG23. No QTL affecting hormonal or biochemical marks was found on LG4 and LG6. Heritability of cortisol, lysozyme levels, and mortality were high (0.36, 0.55, and 0.38, respectively). |
Publications
2021 |
New records of the Indo-Pacific shrimp Urocaridella pulchella Yokeş & Galil, 2006 from the Eastern Mediterranean Sea Journal Article BIR, 10 (2), pp. 295–303, 2021, ISSN: 22421300. |
Mediterranean Marine Science, 2021, ISSN: 1791-6763, 1108-393X. |
Brussels, Belgium, 2021, (BIODIV). |
On single use plastic straws: Pre-ban findings on touristic beaches in Crete Journal Article Marine Pollution Bulletin, 171 , pp. 112790, 2021, ISSN: 0025326X. |
Aquaculture Reports, 20 , pp. 100767, 2021, ISSN: 23525134. |
Phytoplankton Phenology in the Coastal Zone of Cyprus, Based on Remote Sensing and In Situ Observations Journal Article Remote Sensing, 14 (1), pp. 12, 2021, ISSN: 2072-4292. |
Plastics in our ocean as transdisciplinary challenge Journal Article Marine Pollution Bulletin, 164 , pp. 112051, 2021, ISSN: 0025326X. |
Post-Prandial Amino Acid Changes in Gilthead Sea Bream Journal Article Animals, 11 (7), pp. 1889, 2021, ISSN: 2076-2615. |
Pre-digest of unprotected DNA by Benzonase improves the representation of living skin bacteria and efficiently depletes host DNA Journal Article Microbiome, 9 (1), pp. 123, 2021, ISSN: 2049-2618. |
Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Microbiome Journal Article Animals, 11 (3), pp. 862, 2021, ISSN: 2076-2615. |
Projecting climate change impacts on Mediterranean finfish production: a case study in Greece Journal Article Climatic Change, 165 (3), pp. 67, 2021, ISSN: 1573-1480. |
Recent advances in culture-based gut microbiome research Journal Article International Journal of Medical Microbiology, 311 (3), pp. 151485, 2021, ISSN: 14384221. |
Serum Ceramides as Prognostic Biomarkers of Large Thrombus Burden in Patients with STEMI: A Micro-Computed Tomography Study Journal Article Journal of Personalized Medicine, 11 (2), pp. 89, 2021. |
Shallow-water hydrothermalism at Milos (Greece): Nature, distribution, heat fluxes and impact on ecosystems Journal Article Marine Geology, 438 , pp. 106521, 2021, ISSN: 00253227. |
Spawning kinetics and parentage contribution of European sea bass (Dicentrarchus labrax) broodstocks, and influence of GnRHa-induced spawning Journal Article Aquaculture Reports, 21 , pp. 100766, 2021, ISSN: 23525134. |
Fish Physiology and Biochemistry, 47 (2), pp. 281–292, 2021, ISSN: 1573-5168. |
Taxonomic composition and assemblage structure of brachiopods from two submarine caves in the Aegean Sea, Eastern Mediterranean Journal Article The European Zoological Journal, 88 (1), pp. 316–327, 2021, ISSN: 2475-0263. |
Testing of the Salmon Welfare Index Model (SWIM 1.0) as a computational welfare assessment for sea-caged European sea bass Journal Article Italian Journal of Animal Science, 20 (1), pp. 1423–1430, 2021, ISSN: null. |
The Dark mAtteR iNvestigator (DARN) tool: getting to know the known unknowns in COI amplicon data Journal Article Metabarcoding and Metagenomics, 5 , pp. e69657, 2021, ISSN: 2534-9708. |
The Importance of Ocean Literacy in the Mediterranean Region—Steps Towards Blue Sustainability Incollection Koutsopoulos, Kostis C; Stel, Jan H (Ed.): Ocean Literacy: Understanding the Ocean, pp. 197–240, Springer International Publishing, Cham, 2021, ISBN: 978-3-030-70154-3 978-3-030-70155-0, (Series Title: Key Challenges in Geography). |
Mediterranean Marine Science, 22 (2), pp. 218–231, 2021, ISSN: 1791-6763. |
Developmental & Comparative Immunology, 114 , pp. 103803, 2021, ISSN: 0145305X. |
The potential contribution of bloggers to change lifestyle and reduce plastic use and pollution: A small data approach Journal Article Marine Pollution Bulletin, 169 , pp. 112525, 2021, ISSN: 0025326X. |
The Santorini Volcanic Complex as a Valuable Source of Enzymes for Bioenergy Journal Article Energies, 14 (5), pp. 1414, 2021, ISSN: 1996-1073. |
The wall lizards of the Balkan peninsula: Tackling questions at the interface of phylogenomics and population genomics Journal Article Molecular Phylogenetics and Evolution, 159 , pp. 107121, 2021, ISSN: 10557903. |
Toward a Global Public Repository of Community Protocols to Encourage Best Practices in Biomolecular Ocean Observing and Research Journal Article Frontiers in Marine Science, 8 , pp. 1488, 2021, ISSN: 2296-7745. |
Tracking and Analysis of the Movement Behavior of European Seabass (Dicentrarchus labrax) in Aquaculture Systems Journal Article Frontiers in Animal Science, 2 , pp. 79, 2021, ISSN: 2673-6225. |
Trophic Diversity of a Fish Community Associated with a Caulerpa prolifera (Forsskål) Meadow in a Shallow Semi-Enclosed Embayment Journal Article JMSE, 9 (2), pp. 165, 2021, ISSN: 2077-1312. |
Aquaculture, 540 , pp. 736683, 2021, ISSN: 00448486. |
2020 |
A collection of bacterial isolates from the pig intestine reveals functional and taxonomic diversity Journal Article Nature Communications, 11 (1), pp. 6389, 2020, ISSN: 2041-1723. |
Zootaxa, 4896 (3), pp. 373–408, 2020, ISSN: 1175-5334, 1175-5326, (BIODIV). |
Scientific Reports, 10 (1), pp. 12294, 2020, ISSN: 2045-2322. |
Mediterranean Marine Science, 21 (3), pp. 775, 2020, ISSN: 1791-6763, 1108-393X. |
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