Title: Development of an Advanced Integrated Toolbox for in vitro high-throughput screening of quality and phyto-estrogens in feed ingredients for Mediterranean finfish

Funding Source: EPAnEK Aquaculture

Budget IMBBC: 70,000€

Start / End Date: 2020 - 2023

Web site URL:

Project Progress: 100%

Principal Investigator:

Efthimia (Efi) Cotou

Project Members:

Research Directions:

Fish nutrition and feeding

Biomarkers and bioassays


The use of alternative ingredients in fish feeds calls for the development of adequate methodology for the control of their quality. Current methodologies are of analytical character and lacking the capacity to actually determine the dimension of their biological effects. The ultimate goal of the FishPhytoFeed is the development of an advanced integrated toolkit for the in vitro high throughput screening of fish feeds ingredients based on their biological activities and effects. No use of live fish will be required and the toolkit determinations will be combined in a synthetic quality factor that will reflect the effects of ingredients on key biological processes that consist fish growth.