Elisavet Kaitetzidou

This user account status is Approved

2023

Kitsoulis, Christos V; Papadogiannis, Vasileios; Kristoffersen, Jon B; Kaitetzidou, Elisavet; Sterioti, Aspasia; Tsigenopoulos, Costas S; Manousaki, Tereza

Near-chromosome level genome assembly of devil firefish, Pterois miles Journal Article

Peer Community Journal, 3 , pp. e64, 2023, ISSN: 2804-3871.

Links | BibTeX

2022

Kaitetzidou, Elisavet; Gilfillan, Gregor D; Antonopoulou, Efthimia; Sarropoulou, Elena

Sex-biased dynamics of three-spined stickleback (Gasterosteus aculeatus) gene expression patterns Journal Article

Genomics, 114 (1), pp. 266–277, 2022, ISSN: 08887543.

Links | BibTeX

Papadaki, Maria; Kaitetzidou, Elisavet; Papadakis, Ioannis E; Sfakianakis, Dimitris G; Papandroulakis, Nikos; Mylonas, Constantinos C; Sarropoulou, Elena

Temperature-Biased miRNA Expression Patterns during European Sea Bass (Dicentrarchus labrax) Development Journal Article

International Journal of Molecular Sciences, 23 (19), pp. 11164, 2022, ISSN: 1422-0067.

Abstract | Links | BibTeX

@article{papadaki_temperature-biased_2022,
title = {Temperature-Biased miRNA Expression Patterns during European Sea Bass (Dicentrarchus labrax) Development},
author = {Maria Papadaki and Elisavet Kaitetzidou and Ioannis E Papadakis and Dimitris G Sfakianakis and Nikos Papandroulakis and Constantinos C Mylonas and Elena Sarropoulou},
url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/09/2022-Papadaki-IJMS-65.pdf
https://www.mdpi.com/1422-0067/23/19/11164},
doi = {10.3390/ijms231911164},
issn = {1422-0067},
year = {2022},
date = {2022-01-01},
urldate = {2022-09-27},
journal = {International Journal of Molecular Sciences},
volume = {23},
number = {19},
pages = {11164},
abstract = {Environmental effects and, particularly, temperature changes have been demonstrated to influence the activity, function, and well-being of teleosts. Temperature may change seasonally in the wild, and in captivity under aquaculture operations. Moreover, climate change is expected to shift temperature profiles worldwide. MicroRNAs (miRNA) are important temperature-sensitive gene-expression regulators acting at the post-transcriptional level. They are known to be key regulators in development, reproduction, and immune responses. Therefore, early larval development of the European sea bass (Dicentrarchus labrax), one of the most extensively cultured species in Mediterranean aquaculture, was investigated at early rearing temperatures, i.e., 15, 17.5, and 20 °C, in regard to the impact of temperatures on miRNAs through sncRNA high-throughput sequencing but also at the phenotypic level in terms of growth, sex, vision, and skeletal deformities. Expression profiling revealed stage- and temperature-specific miRNA expression targeting genes with roles in reproduction and immune response mainly at the flexion and all-fins stages. Similar stage- and temperature-specific results were also observed concerning the number of rod cells and lower jaw elongation. The present work presents for the first time highly promising results on the influence of early rearing temperature at the post-transcriptional level during European sea bass development, with a putative impact on reproduction and immune response, as well as regarding teleost vision and larval development.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

2021

Zafeiropoulos, Haris; Gioti, Anastasia; Ninidakis, Stelios; Potirakis, Antonis; Paragkamian, Savvas; Angelova, Nelina; Antoniou, Aglaia; Danis, Theodoros; Kaitetzidou, Eliza; Kasapidis, Panagiotis; Kristoffersen, Jon Bent; Papadogiannis, Vasileios; Pavloudi, Christina; Ha, Quoc Viet; Lagnel, Jacques; Pattakos, Nikos; Perantinos, Giorgos; Sidirokastritis, Dimitris; Vavilis, Panagiotis; Kotoulas, Georgios; Manousaki, Tereza; Sarropoulou, Elena; Tsigenopoulos, Costas S; Arvanitidis, Christos; Magoulas, Antonios; Pafilis, Evangelos

0s and 1s in marine molecular research: a regional HPC perspective Journal Article

GigaScience, 10 (8), pp. giab053, 2021, ISSN: 2047-217X.

Abstract | Links | BibTeX

@article{zafeiropoulos_0s_2021,
title = {0s and 1s in marine molecular research: a regional HPC perspective},
author = {Haris Zafeiropoulos and Anastasia Gioti and Stelios Ninidakis and Antonis Potirakis and Savvas Paragkamian and Nelina Angelova and Aglaia Antoniou and Theodoros Danis and Eliza Kaitetzidou and Panagiotis Kasapidis and Jon Bent Kristoffersen and Vasileios Papadogiannis and Christina Pavloudi and Quoc Viet Ha and Jacques Lagnel and Nikos Pattakos and Giorgos Perantinos and Dimitris Sidirokastritis and Panagiotis Vavilis and Georgios Kotoulas and Tereza Manousaki and Elena Sarropoulou and Costas S Tsigenopoulos and Christos Arvanitidis and Antonios Magoulas and Evangelos Pafilis},
url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/08/2021-Zafeiropoulos-GiGa-63.pdf
https://academic.oup.com/gigascience/article/doi/10.1093/gigascience/giab053/6353916},
doi = {10.1093/gigascience/giab053},
issn = {2047-217X},
year = {2021},
date = {2021-01-01},
urldate = {2021-08-23},
journal = {GigaScience},
volume = {10},
number = {8},
pages = {giab053},
abstract = {Abstract
High-performance computing (HPC) systems have become indispensable for modern marine research, providing support to an increasing number and diversity of users. Pairing with the impetus offered by high-throughput methods to key areas such as non-model organism studies, their operation continuously evolves to meet the corresponding computational challenges. Here, we present a Tier 2 (regional) HPC facility, operating for over a decade at the Institute of Marine Biology, Biotechnology, and Aquaculture of the Hellenic Centre for Marine Research in Greece. Strategic choices made in design and upgrades aimed to strike a balance between depth (the need for a few high-memory nodes) and breadth (a number of slimmer nodes), as dictated by the idiosyncrasy of the supported research. Qualitative computational requirement analysis of the latter revealed the diversity of marine fields, methods, and approaches adopted to translate data into knowledge. In addition, hardware and software architectures, usage statistics, policy, and user management aspects of the facility are presented. Drawing upon the last decade’s experience from the different levels of operation of the Institute of Marine Biology, Biotechnology, and Aquaculture HPC facility, a number of lessons are presented; these have contributed to the facility’s future directions in light of emerging distribution technologies (e.g., containers) and Research Infrastructure evolution. In combination with detailed knowledge of the facility usage and its upcoming upgrade, future collaborations in marine research and beyond are envisioned.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

2020

Papadaki, Maria; Kaitetzidou, Elisavet; Mylonas, Constantinos C; Sarropoulou, Elena

Non-coding RNA Expression Patterns of Two Different Teleost Gonad Maturation Stages Journal Article

Marine Biotechnology, 22 (5), pp. 683–695, 2020, ISSN: 1436-2228, 1436-2236.

Links | BibTeX

2019

Kaitetzidou, Elisavet; Katsiadaki, Ioanna; Lagnel, Jacques; Antonopoulou, Efthimia; Sarropoulou, Elena

Unravelling paralogous gene expression dynamics during three-spined stickleback embryogenesis Journal Article

Scientific Reports, 9 (1), pp. 3752, 2019, ISSN: 2045-2322.

Links | BibTeX

Sarropoulou, Elena; Kaitetzidou, Elizabet; Papandroulakis, Nikos; Tsalafouta, Aleka; Pavlidis, Michalis

Inventory of European Sea Bass (Dicentrarchus labrax) sncRNAs Vital During Early Teleost Development Journal Article

Frontiers in Genetics, 10 , pp. 657, 2019, ISSN: 1664-8021.

Links | BibTeX

2017

Sarropoulou, E; Sundaram, A Y M; Kaitetzidou, E; Kotoulas, G; Gilfillan, G D; Papandroulakis, N; Mylonas, C C; Magoulas, A

Full genome survey and dynamics of gene expression in the greater amberjack Seriola dumerili Journal Article

GigaScience, 6 (12), pp. 1–13, 2017, ISSN: 2047217X, (Publisher: Oxford University Press).

Abstract | Links | BibTeX

@article{sarropoulou_full_2017,
title = {Full genome survey and dynamics of gene expression in the greater amberjack Seriola dumerili},
author = {E Sarropoulou and A Y M Sundaram and E Kaitetzidou and G Kotoulas and G D Gilfillan and N Papandroulakis and C C Mylonas and A Magoulas},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042364258&doi=10.1093%2fgigascience%2fgix108&partnerID=40&md5=fa6e269b1e6ff57a26c0c9c6efe45226},
doi = {10.1093/gigascience/gix108},
issn = {2047217X},
year = {2017},
date = {2017-01-01},
journal = {GigaScience},
volume = {6},
number = {12},
pages = {1--13},
abstract = {Background: Teleosts of the genus Seriola, commonly known as amberjacks, are of high commercial value in international markets due to their flesh quality and worldwide distribution. The Seriola species of interest to Mediterranean aquaculture is the greater amberjack (Seriola dumerili). This species holds great potential for the aquaculture industry, but in captivity, reproduction has proved to be challenging, and observed growth dysfunction hinders their domestication. Insights into molecular mechanisms may contribute to a better understanding of traits like growth and sex, but investigations to unravel the molecular background of amberjacks have begun only recently. Findings: Illumina HiSeq sequencing generated a high-coverage greater amberjack genome sequence comprising 45 909 scaffolds. Comparative mapping to the Japanese yellowtail (Seriola quinqueriadiata) and to the model species medaka (Oryzias latipes) allowed the generation of in silico groups. Additional gonad transcriptome sequencing identified sex-biased transcripts, including known sex-determining and differentiation genes. Investigation of the muscle transcriptome of slow-growing individuals showed that transcripts involved in oxygen and gas transport were differentially expressed compared with fast/normal-growing individuals. On the other hand, transcripts involved in muscle functions were found to be enriched in fast/normal-growing individuals. Conclusion: The present study provides the first insights into the molecular background of male and female amberjacks and of fast- and slow-growing fish. Therefore, valuable molecular resources have been generated in the form of a first draft genome and a reference transcriptome. Sex-biased genes, which may also have roles in sex determination or differentiation, and genes that may be responsible for slow growth are suggested. © The Author(s) 2017.},
note = {Publisher: Oxford University Press},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Kaitetzidou, E; Ludwig, A; Gessner, J; Sarropoulou, E

Expression patterns of atlantic sturgeon (Acipenser oxyrinchus) during embryonic development Journal Article

G3: Genes, Genomes, Genetics, 7 (2), pp. 533–542, 2017, ISSN: 21601836, (Publisher: Genetics Society of America).

Abstract | Links | BibTeX

@article{kaitetzidou_expression_2017,
title = {Expression patterns of atlantic sturgeon (Acipenser oxyrinchus) during embryonic development},
author = {E Kaitetzidou and A Ludwig and J Gessner and E Sarropoulou},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85012900590&doi=10.1534%2fg3.116.036699&partnerID=40&md5=a55f7b655c7c43f35caf4131f17e4438},
doi = {10.1534/g3.116.036699},
issn = {21601836},
year = {2017},
date = {2017-01-01},
journal = {G3: Genes, Genomes, Genetics},
volume = {7},
number = {2},
pages = {533--542},
abstract = {During teleost ontogeny the larval and embryonic stages are key stages, since failure during this period of tissue differentiation may cause malformations, developmental delays, poor growth, and massive mortalities. Despite the rapid advances in sequencing technologies, the molecular backgrounds of the development of economically important but endangered fish species like the Atlantic sturgeon (Acipenser oxyrinchus) have not yet been thoroughly investigated. The current study examines the differential expression of transcripts involved in embryonic development of the Atlantic sturgeon. Addressing this goal, a reference transcriptome comprising eight stages was generated using an Illumina HiSequation 2500 platform. The constructed de novo assembly counted to 441,092 unfiltered and 179,564 filtered transcripts. Subsequently, the expression profile of four developmental stages ranging from early (gastrula) to late stages of prelarval development [2 d posthatching (dph)] were investigated applying an Illumina MiSeq platform. Differential expression analysis revealed distinct expression patterns among stages, especially between the two early and the two later stages. Transcripts upregulated at the two early stages were mainly enriched in transcripts linked to developmental processes, while transcripts expressed at the last two stages were mainly enriched in transcripts important to muscle contraction. Furthermore, important stagespecific expression has been detected for the hatching stage with transcripts enriched in molecule transport, and for the 2 dph stage with transcripts enriched in visual perception and lipid digestion. Our investigation represents a significant contribution to the understanding of Atlantic sturgeon embryonic development, and transcript characterization along with the differential expression results will significantly contribute to sturgeon research and aquaculture. © 2017 Kaitetzidou et al.},
note = {Publisher: Genetics Society of America},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Antonopoulou, E; Kaitetzidou, E; Castellana, B; Panteli, N; Kyriakis, D; Vraskou, Y; Planas, J V

In vivo effects of lipopolysaccharide on peroxisome proliferator-activated receptor expression in juvenile gilthead seabream (Sparus Aurata) Journal Article

Biology, 6 (4), 2017, ISSN: 20797737, (Publisher: MDPI AG).

Abstract | Links | BibTeX

@article{antonopoulou_vivo_2017,
title = {In vivo effects of lipopolysaccharide on peroxisome proliferator-activated receptor expression in juvenile gilthead seabream (Sparus Aurata)},
author = {E Antonopoulou and E Kaitetzidou and B Castellana and N Panteli and D Kyriakis and Y Vraskou and J V Planas},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85031111165&doi=10.3390%2fbiology6040036&partnerID=40&md5=2b1f32ff67149b9c6bc74d79a5408c67},
doi = {10.3390/biology6040036},
issn = {20797737},
year = {2017},
date = {2017-01-01},
journal = {Biology},
volume = {6},
number = {4},
abstract = {Fish are constantly exposed to microorganisms in the aquatic environment, many of which are bacterial pathogens. Bacterial pathogens activate the innate immune response in fish involving the production of pro-inflammatory molecules that, in addition to their immune-related role, can affect non-immune tissues. In the present study, we aimed at investigating how inflammatory responses can affect metabolic homeostasis in the gilthead seabream (Sparus aurata), a teleost of considerable economic importance in Southern European countries. Specifically, we mimicked a bacterial infection by in vivo administration of lipopolysaccharide (LPS, 6 mg/kg body weight) and measured metabolic parameters in the blood and, importantly, the mRNA expression levels of the three isotypes of peroxisome proliferator activated receptors (PPARα, β, and γ) in metabolically-relevant tissues in seabream. PPARs are nuclear receptors that are important for lipid and carbohydrate metabolism in mammals and that act as biological sensors of altered lipid metabolism. We show here that LPS-induced inflammatory responses result in the modulation of triglyceride plasma levels that are accompanied most notably by a decrease in the hepatic mRNA expression levels of PPARα, β, and γ and by the up-regulation of PPARγ expression only in adipose tissue and the anterior intestine. In addition, LPS-induced inflammation results in an increase in the hepatic mRNA expression and protein activity levels of members of the mitogen-activated protein kinase (MAPK) family, known in mammals to regulate the transcription and activity of PPARs. Our results provide evidence for the involvement of PPARs in the metabolic response to inflammatory stimuli in seabream and offer insights into the molecular mechanisms underlying the redirection of metabolic activities under inflammatory conditions in vertebrates. © 2017 by the authors. Licensee MDPI, Basel, Switzerland.},
note = {Publisher: MDPI AG},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

2015

Kaitetzidou, E; Chatzifotis, S; Antonopoulou, E; Sarropoulou, E

Identification, Phylogeny, and Function of fabp2 Paralogs in Two Non-Model Teleost Fish Species Journal Article

Marine Biotechnology, 17 (5), pp. 663–677, 2015, ISSN: 14362228, (Publisher: Springer New York LLC).

Abstract | Links | BibTeX

@article{kaitetzidou_identification_2015,
title = {Identification, Phylogeny, and Function of fabp2 Paralogs in Two Non-Model Teleost Fish Species},
author = {E Kaitetzidou and S Chatzifotis and E Antonopoulou and E Sarropoulou},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84939566208&doi=10.1007%2fs10126-015-9648-6&partnerID=40&md5=7ed4057e28f0af24da6033ebec855acb},
doi = {10.1007/s10126-015-9648-6},
issn = {14362228},
year = {2015},
date = {2015-01-01},
journal = {Marine Biotechnology},
volume = {17},
number = {5},
pages = {663--677},
abstract = {Intestinal fatty-acid-binding protein (IFABP or FABP2) is a cytosolic transporter of long-chain fatty acids, which is mainly expressed in cells of intestinal tissue. Fatty acids in teleosts are an important source of energy for growth, reproduction, and swimming and a main ingredient in the yolk sac of embryos and larvae. The fabp2 paralogs, fabp2a and fabp2b, were identified for 26 teleost fish species including the paralogs for the two non-model teleost fish species, namely the gilthead sea bream (Sparus aurata) and the European sea bass (Dicentrarchus labrax). Despite the high similarity of fabp2 paralogs, as well as the identical organization in four exons, paralogs were mapped to different chromosomes/linkage groups supporting the hypothesis that the identified transcripts are true paralogs originating from a single ancestor gene after genome duplication. This was also confirmed by phylogenetic analysis using fabp2 sequences of 26 teleosts and by synteny analysis carried out with ten teleosts. Differential expression analysis of the gilthead sea bream and European sea bass fabp2 paralogs in the intestine after fasting and refeeding experiment further revealed their altered implication in metabolism. Additional expression studies in seven developmental stages of the two species detected fabp2 paralogs relatively early in the embryonic development as well as possible complementary or separated roles of the paralogs. The identification and characterization of the two fabp2 paralogs will contribute significantly to the understanding of the fabp2 evolution as well as of the divergences in fatty acid metabolism. © 2015, Springer Science+Business Media New York.},
note = {Publisher: Springer New York LLC},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Kaitetzidou, E; Xiang, J; Antonopoulou, E; Tsigenopoulos, C S; Sarropoulou, E

Dynamics of gene expression patterns during early development of the European seabass (Dicentrarchus labrax) Journal Article

Physiological Genomics, 47 (5), pp. 158–169, 2015, ISSN: 10948341, (Publisher: American Physiological Society).

Abstract | Links | BibTeX

@article{kaitetzidou_dynamics_2015,
title = {Dynamics of gene expression patterns during early development of the European seabass (Dicentrarchus labrax)},
author = {E Kaitetzidou and J Xiang and E Antonopoulou and C S Tsigenopoulos and E Sarropoulou},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84930978495&doi=10.1152%2fphysiolgenomics.00001.2015&partnerID=40&md5=ac0e811b8dd4948aca47662547595ee0},
doi = {10.1152/physiolgenomics.00001.2015},
issn = {10948341},
year = {2015},
date = {2015-01-01},
journal = {Physiological Genomics},
volume = {47},
number = {5},
pages = {158--169},
abstract = {Larval and embryonic stages are the most critical period in the life cycle of marine fish. Key developmental events occur early in development and are influenced by external parameters like stress, temperature, salinity, and photoperiodism. Any failure may cause malformations, developmental delays, poor growth, and massive mortalities. Advanced understanding of molecular processes underlying marine larval development may lead to superior larval rearing conditions. Today, the new sequencing and bioinformatic methods allow transcriptome screens comprising messenger (mRNA) and microRNA (miRNA) with the scope of detecting differential expression for any species of interest. In the present study, we applied Illumina technology to investigate the transcriptome of early developmental stages of the European seabass (Dicentrarchus labrax). The European seabass, in its natural environment, is a euryhaline species and has shown high adaptation processes in early life phases. During its embryonic and larval phases the European seabass lives in a marine environment and as a juvenile it migrates to coastal zones, estuaries, and lagoons. Investigating the dynamics of gene expression in its early development may shed light on factors promoting phenotypic plasticity and may also contribute to the improvement and advancement of rearing methods of the European seabass, a species of high economic importance in European and Mediterranean aquaculture. We present the identification, characterization, and expression of mRNA and miRNA, comprising paralogous genes and differentially spliced transcripts from early developmental stages of the European seabass. We further investigated the detection of possible interactions of miRNA with mRNA. © 2015 the American Physiological Society.},
note = {Publisher: American Physiological Society},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

2014

Feidantsis, K; Kaitetzidou, E; Mavrogiannis, N; Michaelidis, B; Kotzamanis, Y; Antonopoulou, E

Effect of taurine-enriched diets on the Hsp expression, MAPK activation and the antioxidant defence of the European sea bass (Dicentrarchus labrax) Journal Article

Aquaculture Nutrition, 20 (4), pp. 431–442, 2014, ISSN: 13535773, (Publisher: Blackwell Publishing Ltd).

Abstract | Links | BibTeX

@article{feidantsis_effect_2014,
title = {Effect of taurine-enriched diets on the Hsp expression, MAPK activation and the antioxidant defence of the European sea bass (Dicentrarchus labrax)},
author = {K Feidantsis and E Kaitetzidou and N Mavrogiannis and B Michaelidis and Y Kotzamanis and E Antonopoulou},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84903899185&doi=10.1111%2fanu.12096&partnerID=40&md5=33717104f36c4f17b28f24bd6fd1eb7a},
doi = {10.1111/anu.12096},
issn = {13535773},
year = {2014},
date = {2014-01-01},
journal = {Aquaculture Nutrition},
volume = {20},
number = {4},
pages = {431--442},
abstract = {This study aimed to identify and characterize the cellular and metabolic response in vital organs of Dicentrarchus labrax, after feeding on soy-based diets enriched with different taurine concentrations (T0.2, T0.5, T1.0 w/w). Molecular responses (expression of Hsp70, Hsp90, phosphorylation of p38 mitogen-activated protein kinase-p38MAPK and extracellular signal-regulated kinases-ERK1/2) were addressed. Metabolic and antioxidant capacities such as activities of citrate synthase (CS), malate and lactate dehydrogenase (MDH, L-LDH), superoxide dismutase (SOD), catalase (CAT) and levels of thiobarbituric acid-reactive substances (TBARS) were assessed. The most intense changes were detected in the liver and in the anterior intestine showing that taurine induces important protective biochemical mechanisms. Specifically, in the liver and the anterior intestine, Hsp and MAPK levels, as well as L-LDH activity levels were up-regulated under the effect of taurine diets. T diet had no major effect on the activity levels of CS, SOD and TBARS, except for MDH in the liver and catalase in the anterior intestine, where activity levels of these enzymes were increased under the effect of T0.2 and T1.0, respectively. These results may facilitate monitoring of adequate health status of farmed fish, exploiting the beneficial T effects and could assist at the formulation of aquaculture feeding strategies. © 2014 John Wiley & Sons Ltd.},
note = {Publisher: Blackwell Publishing Ltd},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

2012

Kaitetzidou, E; Crespo, D; Vraskou, Y; Antonopoulou, E; Planas, J V

Transcriptomic Response of Skeletal Muscle to Lipopolysaccharide in the Gilthead Seabream (Sparus aurata) Journal Article

Marine Biotechnology, 14 (5), pp. 605–619, 2012, ISSN: 14362228.

Abstract | Links | BibTeX

@article{kaitetzidou_transcriptomic_2012,
title = {Transcriptomic Response of Skeletal Muscle to Lipopolysaccharide in the Gilthead Seabream (Sparus aurata)},
author = {E Kaitetzidou and D Crespo and Y Vraskou and E Antonopoulou and J V Planas},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84865418445&doi=10.1007%2fs10126-012-9469-9&partnerID=40&md5=918d6640000b1341c4c31b45ff33c978},
doi = {10.1007/s10126-012-9469-9},
issn = {14362228},
year = {2012},
date = {2012-01-01},
journal = {Marine Biotechnology},
volume = {14},
number = {5},
pages = {605--619},
abstract = {The physiological consequences of the activation of the immune system in fish are not well understood. In particular, skeletal muscle, due to its essential role in locomotion and whole-animal energy homeostasis, is a potentially important target of inflammation. In this study, we have evaluated the in vivo effects of lipopolysaccharide (LPS) on the white and red skeletal muscle transcriptome of the gilthead seabream (Sparus aurata) by microarray analysis at 24 and 72 h after injection. In white muscle, the transcriptomic response was characterized by an up-regulation of genes involved in carbohydrate catabolism and protein synthesis at 24 h and a complete reversal of this pattern at 72 h. In red muscle, an up-regulation of genes involved in carbohydrate catabolism and protein synthesis was observed only at 72 h after LPS administration. Interestingly, both white and red muscles showed a similar consistent down-regulation of immune genes at 72 h post-injection. However, genes involved in muscle contraction showed a general up-regulation in response to LPS in both types of muscle. In summary, LPS administration causes muscle type-specific responses regarding the expression of genes involved in carbohydrate and protein metabolism and a common decreased expression of immune genes in skeletal muscle, concomitant with increased expression of genes for contractile elements. Our results evidence a robust and tissue-specific transcriptomic response of the skeletal muscle to an acute inflammatory challenge. © 2012 Springer Science+Business Media, LLC.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}