2024 |
Skliros, Dimitrios; Kostakou, Maria; Kokkari, Constantina; Tsertou, Maria Ioanna; Pavloudi, Christina; Zafeiropoulos, Haris; Katharios, Pantelis; Flemetakis, Emmanouil Microorganisms, 12 (11), pp. 2281, 2024, ISSN: 2076-2607. @article{skliros_unveiling_2024, title = {Unveiling Emerging Opportunistic Fish Pathogens in Aquaculture: A Comprehensive Seasonal Study of Microbial Composition in Mediterranean Fish Hatcheries}, author = {Dimitrios Skliros and Maria Kostakou and Constantina Kokkari and Maria Ioanna Tsertou and Christina Pavloudi and Haris Zafeiropoulos and Pantelis Katharios and Emmanouil Flemetakis}, url = {https://www.mdpi.com/2076-2607/12/11/2281 https://imbbc.hcmr.gr/wp-content/uploads/2024/11/2024-Skliros-microorganisms-45.pdf}, doi = {10.3390/microorganisms12112281}, issn = {2076-2607}, year = {2024}, date = {2024-11-12}, urldate = {2024-11-12}, journal = {Microorganisms}, volume = {12}, number = {11}, pages = {2281}, abstract = {The importance of microbial communities in fish hatcheries for fish health and welfare has been recognized, with several studies mapping these communities during healthy rearing conditions and disease outbreaks. In this study, we analyzed the bacteriome of the live feeds, such as microalgae, rotifers, and Artemia, used in fish hatcheries that produce Mediterranean species. Our goal was to provide baseline information about their structure, emphasizing in environmental putative fish pathogenic bacteria. We conducted 16S rRNA amplicon Novaseq sequencing for our analysis, and we inferred 46,745 taxonomically annotated ASVs. Results showed that incoming environmental water plays a significant role in the presence of important taxa that constitute presumptive pathogens. Bio-statistical analyses revealed a relatively stable bacteriome among seasonal samplings for every hatchery but a diverse bacteriome between sampling stations and a distinct core bacteriome for each hatchery. Analysis of putative opportunistic fish pathogenic genera revealed some co-occurrence correlation events and a high average relative abundance of Vibrio, Tenacibaculum, and Photobacterium genera in live feeds, reaching a grand mean average of up to 7.3% for the hatchery of the Hellenic Center of Marine Research (HCMR), 12% for Hatchery A, and 11.5% for Hatchery B. Mapping the bacteriome in live feeds is pivotal for understanding the marine environment and distinct aquaculture practices and can guide improvements in hatchery management, enhancing fish health and sustainability in the Mediterranean region.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The importance of microbial communities in fish hatcheries for fish health and welfare has been recognized, with several studies mapping these communities during healthy rearing conditions and disease outbreaks. In this study, we analyzed the bacteriome of the live feeds, such as microalgae, rotifers, and Artemia, used in fish hatcheries that produce Mediterranean species. Our goal was to provide baseline information about their structure, emphasizing in environmental putative fish pathogenic bacteria. We conducted 16S rRNA amplicon Novaseq sequencing for our analysis, and we inferred 46,745 taxonomically annotated ASVs. Results showed that incoming environmental water plays a significant role in the presence of important taxa that constitute presumptive pathogens. Bio-statistical analyses revealed a relatively stable bacteriome among seasonal samplings for every hatchery but a diverse bacteriome between sampling stations and a distinct core bacteriome for each hatchery. Analysis of putative opportunistic fish pathogenic genera revealed some co-occurrence correlation events and a high average relative abundance of Vibrio, Tenacibaculum, and Photobacterium genera in live feeds, reaching a grand mean average of up to 7.3% for the hatchery of the Hellenic Center of Marine Research (HCMR), 12% for Hatchery A, and 11.5% for Hatchery B. Mapping the bacteriome in live feeds is pivotal for understanding the marine environment and distinct aquaculture practices and can guide improvements in hatchery management, enhancing fish health and sustainability in the Mediterranean region. |
Zrnčić, Snježana; Katharios, Pantelis; Padrós, Francesc; Fioravanti, Marialetizia; Gustinelli, Andrea; Breton, Alain Le; Toffan, Anna New Challenges and Achievements in Mediterranean Fish Health Management Journal Article Bulletin of the European Association of Fish Pathologists, 2024, ISSN: 3005-4648, 0108-0288. @article{zrncic_new_2024, title = {New Challenges and Achievements in Mediterranean Fish Health Management}, author = {Snježana Zrnčić and Pantelis Katharios and Francesc Padrós and Marialetizia Fioravanti and Andrea Gustinelli and Alain Le Breton and Anna Toffan}, url = {https://eafpbulletin.scholasticahq.com/article/118459-new-challenges-and-achievements-in-mediterranean-fish-health-management https://imbbc.hcmr.gr/wp-content/uploads/2024/07/2024-Katharios-Bulletin-of-EAFP-36.pdf}, doi = {10.48045/001c.118459}, issn = {3005-4648, 0108-0288}, year = {2024}, date = {2024-07-26}, urldate = {2024-07-26}, journal = {Bulletin of the European Association of Fish Pathologists}, abstract = {The European seabass ( Dicentrarchus labrax ) and gilthead seabream ( Sparus aurata ) are key species in marine aquaculture, cultivated in both EU and North African/Levant Mediterranean regions. Production disparities between these areas emphasize a significant reliance on trade for juveniles, feed, and other resources, posing notable biosecurity risks. Addressing shared health concerns across the Mediterranean requires a regional approach to ensure successful management. A recent workshop delved into pivotal topics, including insights from concluded Horizon 2020 projects, effectiveness of nutraceuticals in combating parasitic infections, legal considerations surrounding autogenous vaccines, updated knowledge in betanodavirus infections, and biosecurity measures in Mediterranean aquaculture. Discussions also explored upcoming challenges such as sustainability in cage-based production, impact of climate change, enhancing diagnostic capabilities, fortifying biosecurity measures, advocating for education, and fostering networking opportunities.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The European seabass ( Dicentrarchus labrax ) and gilthead seabream ( Sparus aurata ) are key species in marine aquaculture, cultivated in both EU and North African/Levant Mediterranean regions. Production disparities between these areas emphasize a significant reliance on trade for juveniles, feed, and other resources, posing notable biosecurity risks. Addressing shared health concerns across the Mediterranean requires a regional approach to ensure successful management. A recent workshop delved into pivotal topics, including insights from concluded Horizon 2020 projects, effectiveness of nutraceuticals in combating parasitic infections, legal considerations surrounding autogenous vaccines, updated knowledge in betanodavirus infections, and biosecurity measures in Mediterranean aquaculture. Discussions also explored upcoming challenges such as sustainability in cage-based production, impact of climate change, enhancing diagnostic capabilities, fortifying biosecurity measures, advocating for education, and fostering networking opportunities. |
Triga, Adriana; Issa, Zeenat Atinuke; Smyrli, Maria; Fenske, Linda; Katharios, Pantelis Virulence and pangenome analysis of Vibrio harveyi strains from Greek and Red Sea marine aquaculture Journal Article Aquaculture, 587 , pp. 740839, 2024, ISSN: 00448486. @article{triga_virulence_2024, title = {Virulence and pangenome analysis of Vibrio harveyi strains from Greek and Red Sea marine aquaculture}, author = {Adriana Triga and Zeenat Atinuke Issa and Maria Smyrli and Linda Fenske and Pantelis Katharios}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2024/04/2024-Triga-AQUA-preprint-22.pdf https://linkinghub.elsevier.com/retrieve/pii/S0044848624003004}, doi = {10.1016/j.aquaculture.2024.740839}, issn = {00448486}, year = {2024}, date = {2024-04-19}, urldate = {2024-04-19}, journal = {Aquaculture}, volume = {587}, pages = {740839}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2023 |
Villa, Yanelys Cantillo; Triga, Adriana; Katharios, Pantelis Polyinfection in Fish Aeromoniasis: A Study of Co-Isolated Aeromonas Species in Aeromonas veronii Outbreaks Journal Article Pathogens, 12 (11), pp. 1337, 2023, ISSN: 2076-0817. @article{cantillo_villa_polyinfection_2023, title = {Polyinfection in Fish Aeromoniasis: A Study of Co-Isolated Aeromonas Species in Aeromonas veronii Outbreaks}, author = {Yanelys Cantillo Villa and Adriana Triga and Pantelis Katharios}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/11/2023-Cantillo-Villa-pathogens-58.pdf https://www.mdpi.com/2076-0817/12/11/1337}, doi = {10.3390/pathogens12111337}, issn = {2076-0817}, year = {2023}, date = {2023-11-13}, urldate = {2023-11-14}, journal = {Pathogens}, volume = {12}, number = {11}, pages = {1337}, abstract = {We studied the phenotypic and genomic characteristics related to the virulence and antibiotic resistance of two Aeromonas strains, which were co-isolated before an outbreak of Aeromonas veronii among diseased seabass on Agathonisi Island, Greece, in April 2015. The first strain, AG2.13.2, is a potentially pathogenic mesophilic variant of Aeromonas salmonicida, and the second, AG2.13.5, corresponds to an Aeromonas rivipollensis related to A. rivipollensis KN-Mc-11N1 with an ANI value of 97.32%. AG2.13.2 lacks the type III secretion system just like other mesophilic strains of A. salmonicida. This characteristic has been associated with lower virulence. However, the genome of AG2.13.2 contains other important virulence factors such as type II and type VI secretion systems, and toxins such as rtxA, aerolysin aer/act, and different types of hemolysins. The strain also carries several genes associated with antibiotic resistance such as the tetE efflux pump, and exhibits resistance to tetracycline, ampicillin, and oxolinic acid. In an in vivo challenge test with gilthead seabream larvae, the A. veronii bv sobria strain AG5.28.6 exhibited the highest virulence among all tested strains. Conversely, both A. salmonicida and A. rivipollensis showed minimal virulence when administered alone. Interestingly, when A. veronii bv sobria AG5.28.6 was co-administered with A. rivipollensis, the larvae survival probability increased compared to those exposed to A. veronii bv sobria AG5.28.6 alone. This finding indicates an antagonistic interaction between A. veronii bv sobria AG5.28.6 and A. rivipollensis AG2.13.5. The co-administration of A. veronii bv sobria AG5.28.6 with Aeromonas salmonicida did not yield distinct survival probabilities. Our results validate that the primary pathogen responsible for European seabass aeromoniasis is Aeromonas veronii bv sobria.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We studied the phenotypic and genomic characteristics related to the virulence and antibiotic resistance of two Aeromonas strains, which were co-isolated before an outbreak of Aeromonas veronii among diseased seabass on Agathonisi Island, Greece, in April 2015. The first strain, AG2.13.2, is a potentially pathogenic mesophilic variant of Aeromonas salmonicida, and the second, AG2.13.5, corresponds to an Aeromonas rivipollensis related to A. rivipollensis KN-Mc-11N1 with an ANI value of 97.32%. AG2.13.2 lacks the type III secretion system just like other mesophilic strains of A. salmonicida. This characteristic has been associated with lower virulence. However, the genome of AG2.13.2 contains other important virulence factors such as type II and type VI secretion systems, and toxins such as rtxA, aerolysin aer/act, and different types of hemolysins. The strain also carries several genes associated with antibiotic resistance such as the tetE efflux pump, and exhibits resistance to tetracycline, ampicillin, and oxolinic acid. In an in vivo challenge test with gilthead seabream larvae, the A. veronii bv sobria strain AG5.28.6 exhibited the highest virulence among all tested strains. Conversely, both A. salmonicida and A. rivipollensis showed minimal virulence when administered alone. Interestingly, when A. veronii bv sobria AG5.28.6 was co-administered with A. rivipollensis, the larvae survival probability increased compared to those exposed to A. veronii bv sobria AG5.28.6 alone. This finding indicates an antagonistic interaction between A. veronii bv sobria AG5.28.6 and A. rivipollensis AG2.13.5. The co-administration of A. veronii bv sobria AG5.28.6 with Aeromonas salmonicida did not yield distinct survival probabilities. Our results validate that the primary pathogen responsible for European seabass aeromoniasis is Aeromonas veronii bv sobria. |
Skliros, Dimitrios; Droubogiannis, Stavros; Kalloniati, Chrysanthi; Katharios, Pantelis; Flemetakis, Emmanouil Perturbation of Quorum Sensing after the Acquisition of Bacteriophage Resistance Could Contribute to Novel Traits in Vibrio alginolyticus Journal Article Microorganisms, 11 (9), pp. 2273, 2023, ISSN: 2076-2607. @article{skliros_perturbation_2023, title = {Perturbation of Quorum Sensing after the Acquisition of Bacteriophage Resistance Could Contribute to Novel Traits in Vibrio alginolyticus}, author = {Dimitrios Skliros and Stavros Droubogiannis and Chrysanthi Kalloniati and Pantelis Katharios and Emmanouil Flemetakis}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/10/2023-Skliros-MicroO-51.pdf https://www.mdpi.com/2076-2607/11/9/2273}, doi = {10.3390/microorganisms11092273}, issn = {2076-2607}, year = {2023}, date = {2023-10-02}, urldate = {2023-10-04}, journal = {Microorganisms}, volume = {11}, number = {9}, pages = {2273}, abstract = {Bacteria employ a wide range of molecular mechanisms to confer resistance to bacteriophages, and these mechanisms are continuously being discovered and characterized. However, there are instances where certain bacterial species, despite lacking these known mechanisms, can still develop bacteriophage resistance through intricate metabolic adaptation strategies, potentially involving mutations in transcriptional regulators or phage receptors. Vibrio species have been particularly useful for studying the orchestrated metabolic responses of Gram-negative marine bacteria in various challenges. In a previous study, we demonstrated that Vibrio alginolyticus downregulates the expression of specific receptors and transporters in its membrane, which may enable the bacterium to evade infection by lytic bacteriophages. In our current study, our objective was to explore how the development of bacteriophage resistance in Vibrio species disrupts the quorum-sensing cascade, subsequently affecting bacterial physiology and metabolic capacity. Using a real-time quantitative PCR (rt-QPCR) platform, we examined the expression pattern of quorum-sensing genes, auto-inducer biosynthesis genes, and cell density regulatory proteins in phage-resistant strains. Our results revealed that bacteriophage-resistant bacteria downregulate the expression of quorum-sensing regulatory proteins, such as LuxM, LuxN, and LuxP. This downregulation attenuates the normal perception of quorum-sensing peptides and subsequently diminishes the expression of cell density regulatory proteins, including LuxU, aphA, and LuxR. These findings align with the diverse phenotypic traits observed in the phage-resistant strains, such as altered biofilm formation, reduced planktonic growth, and reduced virulence. Moreover, the transcriptional depletion of aphA, the master regulator associated with low cell density, was linked to the downregulation of genes related to virulence. This phenomenon appears to be phage-specific, suggesting a finely tuned metabolic adaptation driven by phage–host interaction. These findings contribute to our understanding of the role of Vibrio species in microbial marine ecology and highlight the complex interplay between phage resistance, quorum sensing, and bacterial physiology.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Bacteria employ a wide range of molecular mechanisms to confer resistance to bacteriophages, and these mechanisms are continuously being discovered and characterized. However, there are instances where certain bacterial species, despite lacking these known mechanisms, can still develop bacteriophage resistance through intricate metabolic adaptation strategies, potentially involving mutations in transcriptional regulators or phage receptors. Vibrio species have been particularly useful for studying the orchestrated metabolic responses of Gram-negative marine bacteria in various challenges. In a previous study, we demonstrated that Vibrio alginolyticus downregulates the expression of specific receptors and transporters in its membrane, which may enable the bacterium to evade infection by lytic bacteriophages. In our current study, our objective was to explore how the development of bacteriophage resistance in Vibrio species disrupts the quorum-sensing cascade, subsequently affecting bacterial physiology and metabolic capacity. Using a real-time quantitative PCR (rt-QPCR) platform, we examined the expression pattern of quorum-sensing genes, auto-inducer biosynthesis genes, and cell density regulatory proteins in phage-resistant strains. Our results revealed that bacteriophage-resistant bacteria downregulate the expression of quorum-sensing regulatory proteins, such as LuxM, LuxN, and LuxP. This downregulation attenuates the normal perception of quorum-sensing peptides and subsequently diminishes the expression of cell density regulatory proteins, including LuxU, aphA, and LuxR. These findings align with the diverse phenotypic traits observed in the phage-resistant strains, such as altered biofilm formation, reduced planktonic growth, and reduced virulence. Moreover, the transcriptional depletion of aphA, the master regulator associated with low cell density, was linked to the downregulation of genes related to virulence. This phenomenon appears to be phage-specific, suggesting a finely tuned metabolic adaptation driven by phage–host interaction. These findings contribute to our understanding of the role of Vibrio species in microbial marine ecology and highlight the complex interplay between phage resistance, quorum sensing, and bacterial physiology. |
Mladineo, Ivona; Volpatti, Donatella; Beraldo, Paola; Rigos, George; Katharios, Pantelis; Padros, Francesc Monogenean Sparicotyle chrysophrii : The major pathogen of the Mediterranean gilthead seabream aquaculture Journal Article Reviews in Aquaculture, pp. raq.12839, 2023, ISSN: 1753-5123, 1753-5131. @article{mladineo_monogenean_2023, title = {Monogenean \textit{Sparicotyle chrysophrii} : The major pathogen of the Mediterranean gilthead seabream aquaculture}, author = {Ivona Mladineo and Donatella Volpatti and Paola Beraldo and George Rigos and Pantelis Katharios and Francesc Padros}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/06/2023-Mladineo-RevAqua-33.pdf https://onlinelibrary.wiley.com/doi/10.1111/raq.12839}, doi = {10.1111/raq.12839}, issn = {1753-5123, 1753-5131}, year = {2023}, date = {2023-06-26}, urldate = {2023-06-26}, journal = {Reviews in Aquaculture}, pages = {raq.12839}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Triga, Adriana; Smyrli, Maria; Katharios, Pantelis Microorganisms, 11 (5), pp. 1197, 2023, ISSN: 2076-2607. @article{triga_pathogenic_2023, title = {Pathogenic and Opportunistic Vibrio spp. Associated with Vibriosis Incidences in the Greek Aquaculture: The Role of Vibrio harveyi as the Principal Cause of Vibriosis}, author = {Adriana Triga and Maria Smyrli and Pantelis Katharios}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/05/2023-Triga-microorganisms-19.pdf https://www.mdpi.com/2076-2607/11/5/1197}, doi = {10.3390/microorganisms11051197}, issn = {2076-2607}, year = {2023}, date = {2023-05-17}, urldate = {2023-05-17}, journal = {Microorganisms}, volume = {11}, number = {5}, pages = {1197}, abstract = {A monitoring program to follow vibriosis incidents in the Greek marine aquaculture was implemented over the past 13 years. 273 isolates, from various cases originating from eight regions and nine hosts, were collected and characterized. The main aquaculture species of the survey were the European seabass (Dicentrarchus labrax) and the gilthead seabream (Sparus aurata). Various species of Vibrionaceae were associated with vibriosis. Vibrio harveyi had the highest prevalence and was isolated throughout the year from all hosts. During the warm months, Vibrio harveyi prevailed with frequent co-isolations of Photobacterium damselae subsp. damselae and Vibrio alginolyticus, while during spring, other Vibrio species were more abundant, such as Vibrio lentus, Vibrio cyclitrophicus, and Vibrio gigantis. Phylogenetic analysis using the mreB gene and the metabolic fingerprint of the isolates showed great variability within the species of the collection. The severity of the disease and the frequency of outbreaks make vibriosis (that is, mainly attributed to V. harveyi) an important concern for the regional aquaculture sector.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A monitoring program to follow vibriosis incidents in the Greek marine aquaculture was implemented over the past 13 years. 273 isolates, from various cases originating from eight regions and nine hosts, were collected and characterized. The main aquaculture species of the survey were the European seabass (Dicentrarchus labrax) and the gilthead seabream (Sparus aurata). Various species of Vibrionaceae were associated with vibriosis. Vibrio harveyi had the highest prevalence and was isolated throughout the year from all hosts. During the warm months, Vibrio harveyi prevailed with frequent co-isolations of Photobacterium damselae subsp. damselae and Vibrio alginolyticus, while during spring, other Vibrio species were more abundant, such as Vibrio lentus, Vibrio cyclitrophicus, and Vibrio gigantis. Phylogenetic analysis using the mreB gene and the metabolic fingerprint of the isolates showed great variability within the species of the collection. The severity of the disease and the frequency of outbreaks make vibriosis (that is, mainly attributed to V. harveyi) an important concern for the regional aquaculture sector. |
Droubogiannis, Stavros; Pavlidi, Lydia; Skliros, Dimitrios; Flemetakis, Emmanouil; Katharios, Pantelis International Journal of Molecular Sciences, 24 (9), pp. 8200, 2023, ISSN: 1422-0067. @article{droubogiannis_comprehensive_2023, title = {Comprehensive Characterization of a Novel Bacteriophage, vB_VhaS_MAG7 against a Fish Pathogenic Strain of Vibrio harveyi and Its In Vivo Efficacy in Phage Therapy Trials}, author = {Stavros Droubogiannis and Lydia Pavlidi and Dimitrios Skliros and Emmanouil Flemetakis and Pantelis Katharios}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/05/2023-Droumbogiannis-IJMarSci-18.pdf https://www.mdpi.com/1422-0067/24/9/8200}, doi = {10.3390/ijms24098200}, issn = {1422-0067}, year = {2023}, date = {2023-05-08}, urldate = {2023-05-08}, journal = {International Journal of Molecular Sciences}, volume = {24}, number = {9}, pages = {8200}, abstract = {Vibrio harveyi, a significant opportunistic marine pathogen, has been a challenge to the aquaculture industry, leading to severe economical and production losses. Phage therapy has been an auspicious approach in controlling such bacterial infections in the era of antimicrobial resistance. In this study, we isolated and fully characterized a novel strain-specific phage, vB_VhaS_MAG7, which infects V. harveyi MM46, and tested its efficacy as a therapeutic agent in challenged gilthead seabream larvae. vB_VhaS_MAG7 is a tailed bacteriophage with a double-stranded DNA of 49,315 bp. No genes linked with virulence or antibiotic resistance were harbored in the genome. The phage had a remarkably large burst size of 1393 PFU cell−1 and showed strong lytic ability in in vitro assays. When applied in phage therapy trials in challenged gilthead seabream larvae, vB_VhaS_MAG7 was capable of improving the survival of the larvae up to 20%. Due to its distinct features and safety, vB_VhaS_MAG7 is considered a suitable candidate for applied phage therapy.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Vibrio harveyi, a significant opportunistic marine pathogen, has been a challenge to the aquaculture industry, leading to severe economical and production losses. Phage therapy has been an auspicious approach in controlling such bacterial infections in the era of antimicrobial resistance. In this study, we isolated and fully characterized a novel strain-specific phage, vB_VhaS_MAG7, which infects V. harveyi MM46, and tested its efficacy as a therapeutic agent in challenged gilthead seabream larvae. vB_VhaS_MAG7 is a tailed bacteriophage with a double-stranded DNA of 49,315 bp. No genes linked with virulence or antibiotic resistance were harbored in the genome. The phage had a remarkably large burst size of 1393 PFU cell−1 and showed strong lytic ability in in vitro assays. When applied in phage therapy trials in challenged gilthead seabream larvae, vB_VhaS_MAG7 was capable of improving the survival of the larvae up to 20%. Due to its distinct features and safety, vB_VhaS_MAG7 is considered a suitable candidate for applied phage therapy. |
Skliros, Dimitrios; Papazoglou, Polyxeni; Gkizi, Danai; Paraskevopoulou, Eleni; Katharios, Pantelis; Goumas, Dimitrios E; Tjamos, Sotirios; Flemetakis, Emmanouil In planta interactions of a novel bacteriophage against Pseudomonas syringae pv. tomato Journal Article Applied Microbiology and Biotechnology, 2023, ISSN: 0175-7598, 1432-0614. @article{skliros_planta_2023, title = {In planta interactions of a novel bacteriophage against Pseudomonas syringae pv. tomato}, author = {Dimitrios Skliros and Polyxeni Papazoglou and Danai Gkizi and Eleni Paraskevopoulou and Pantelis Katharios and Dimitrios E Goumas and Sotirios Tjamos and Emmanouil Flemetakis}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/04/2023-Skliros-AMB-16.pdf https://link.springer.com/10.1007/s00253-023-12493-5}, doi = {10.1007/s00253-023-12493-5}, issn = {0175-7598, 1432-0614}, year = {2023}, date = {2023-04-27}, urldate = {2023-04-27}, journal = {Applied Microbiology and Biotechnology}, abstract = {Abstract The biology and biotechnology of bacteriophages have been extensively studied in recent years to explore new and environmentally friendly methods of controlling phytopathogenic bacteria. Pseudomonas syringae pv. tomato (Pst) is responsible for bacterial speck disease in tomato plants, leading to decreased yield. Disease management strategies rely on the use of copper-based pesticides. The biological control of Pst with the use of bacteriophages could be an alternative environmentally friendly approach to diminish the detrimental effects of Pst in tomato cultivations. The lytic efficacy of bacteriophages can be used in biocontrol-based disease management strategies. Here, we report the isolation and complete characterization of a bacteriophage, named Medea1, which was also tested in planta against Pst, under greenhouse conditions. The application of Medea1 as a root drenching inoculum or foliar spraying reduced 2.5- and fourfold on average, respectively, Pst symptoms in tomato plants, compared to a control group. In addition, it was observed that defense-related genes PR1b and Pin2 were upregulated in the phage-treated plants. Our research explores a new genus of Pseudomonas phages and explores its biocontrol potential against Pst, by utilizing its lytic nature and ability to trigger the immune response of plants. Key points • Medea1 is a newly reported bacteriophage against Pseudomonas syringae pv. tomato having genomic similarities with the phiPSA1 bacteriophage • Two application strategies were reported, one by root drenching the plants with a phage-based solution and one by foliar spraying, showing up to 60- and 6-fold reduction of Pst population and disease severity in some cases, respectively, compared to control • Bacteriophage Medea1 induced the expression of the plant defense-related genes Pin2 and PR1b}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract The biology and biotechnology of bacteriophages have been extensively studied in recent years to explore new and environmentally friendly methods of controlling phytopathogenic bacteria. Pseudomonas syringae pv. tomato (Pst) is responsible for bacterial speck disease in tomato plants, leading to decreased yield. Disease management strategies rely on the use of copper-based pesticides. The biological control of Pst with the use of bacteriophages could be an alternative environmentally friendly approach to diminish the detrimental effects of Pst in tomato cultivations. The lytic efficacy of bacteriophages can be used in biocontrol-based disease management strategies. Here, we report the isolation and complete characterization of a bacteriophage, named Medea1, which was also tested in planta against Pst, under greenhouse conditions. The application of Medea1 as a root drenching inoculum or foliar spraying reduced 2.5- and fourfold on average, respectively, Pst symptoms in tomato plants, compared to a control group. In addition, it was observed that defense-related genes PR1b and Pin2 were upregulated in the phage-treated plants. Our research explores a new genus of Pseudomonas phages and explores its biocontrol potential against Pst, by utilizing its lytic nature and ability to trigger the immune response of plants. Key points • Medea1 is a newly reported bacteriophage against Pseudomonas syringae pv. tomato having genomic similarities with the phiPSA1 bacteriophage • Two application strategies were reported, one by root drenching the plants with a phage-based solution and one by foliar spraying, showing up to 60- and 6-fold reduction of Pst population and disease severity in some cases, respectively, compared to control • Bacteriophage Medea1 induced the expression of the plant defense-related genes Pin2 and PR1b |
Tsertou, Maria Ioanna; Triga, Adriana; Droubogiannis, Stavros; Kokkari, Constantina; Anasi, Grammatiki; Katharios, Pantelis Frontiers in Microbiology, 14 , pp. 1078669, 2023, ISSN: 1664-302X. @article{tsertou_isolation_2023, title = {Isolation and characterization of a novel Tenacibaculum species and a corresponding bacteriophage from a Mediterranean fish hatchery: Description of Tenacibaculum larymnensis sp. nov. and Tenacibaculum phage Larrie}, author = {Maria Ioanna Tsertou and Adriana Triga and Stavros Droubogiannis and Constantina Kokkari and Grammatiki Anasi and Pantelis Katharios}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/03/2023-Tsertou-FroMicro-13.pdf https://www.frontiersin.org/articles/10.3389/fmicb.2023.1078669/full}, doi = {10.3389/fmicb.2023.1078669}, issn = {1664-302X}, year = {2023}, date = {2023-03-07}, urldate = {2023-03-07}, journal = {Frontiers in Microbiology}, volume = {14}, pages = {1078669}, abstract = {Tenacibaculum larymnensis sp. nov., a novel species of the Tenacibaculum genus was isolated from a commercial fish hatchery in Greece. The novel species is phylogenetically close to T. discolor and was biochemically and genetically characterized. The genome of T . larymnensis has 3.66 Mbps length, 31.83% GC content and the genomic analysis demonstrated that it harbors a wide enzymatic repertoire suggestive of increased degrading capacity but also several virulence factors including hemolysins, secretion systems, transporters, siderophores, pili and extracellular proteins. Using the novel strain, a virulent bacteriophage designated as Tenacibaculum phage Larrie was isolated and characterized. Larrie is a novel Siphovirus with relatively large genome, 77.5 kbps with 111 ORFs, a GC content of 33.7% and an exclusively lytic lifestyle. The new phage-host system can serve as an efficient model to study microbial interactions in the aquatic environment which contribute to the nutrient cycling.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Tenacibaculum larymnensis sp. nov., a novel species of the Tenacibaculum genus was isolated from a commercial fish hatchery in Greece. The novel species is phylogenetically close to T. discolor and was biochemically and genetically characterized. The genome of T . larymnensis has 3.66 Mbps length, 31.83% GC content and the genomic analysis demonstrated that it harbors a wide enzymatic repertoire suggestive of increased degrading capacity but also several virulence factors including hemolysins, secretion systems, transporters, siderophores, pili and extracellular proteins. Using the novel strain, a virulent bacteriophage designated as Tenacibaculum phage Larrie was isolated and characterized. Larrie is a novel Siphovirus with relatively large genome, 77.5 kbps with 111 ORFs, a GC content of 33.7% and an exclusively lytic lifestyle. The new phage-host system can serve as an efficient model to study microbial interactions in the aquatic environment which contribute to the nutrient cycling. |
Kalatzis, Panos G; Mauritzen, Jesper Juel; Winther-Have, Caroline Sophie; Michniewski, Slawomir; Millard, Andrew; Tsertou, Maria Ioanna; Katharios, Pantelis; Middelboe, Mathias International Journal of Molecular Sciences, 24 (4), pp. 3937, 2023, ISSN: 1422-0067. @article{kalatzis_staying_2023, title = {Staying below the Radar: Unraveling a New Family of Ubiquitous “Cryptic” Non-Tailed Temperate Vibriophages and Implications for Their Bacterial Hosts}, author = {Panos G Kalatzis and Jesper Juel Mauritzen and Caroline Sophie Winther-Have and Slawomir Michniewski and Andrew Millard and Maria Ioanna Tsertou and Pantelis Katharios and Mathias Middelboe}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/02/2023-Kalatzis-IJMolSci-11.pdf https://www.mdpi.com/1422-0067/24/4/3937}, doi = {10.3390/ijms24043937}, issn = {1422-0067}, year = {2023}, date = {2023-02-17}, urldate = {2023-02-20}, journal = {International Journal of Molecular Sciences}, volume = {24}, number = {4}, pages = {3937}, abstract = {Bacteriophages are the most abundant biological entities in the oceans and play key roles in bacterial activity, diversity and evolution. While extensive research has been conducted on the role of tailed viruses (Class: Caudoviricetes), very little is known about the distribution and functions of the non-tailed viruses (Class: Tectiliviricetes). The recent discovery of the lytic Autolykiviridae family demonstrated the potential importance of this structural lineage, emphasizing the need for further exploration of the role of this group of marine viruses. Here, we report the novel family of temperate phages under the class of Tectiliviricetes, which we propose to name “Asemoviridae” with phage NO16 as a main representative. These phages are widely distributed across geographical regions and isolation sources and found inside the genomes of at least 30 species of Vibrio, in addition to the original V. anguillarum isolation host. Genomic analysis identified dif-like sites, suggesting that NO16 prophages recombine with the bacterial genome based on the XerCD site-specific recombination mechanism. The interactions between the NO16 phage and its V. anguillarum host were linked to cell density and phage–host ratio. High cell density and low phage predation levels were shown to favor the temperate over the lytic lifestyle for NO16 viruses, and their spontaneous induction rate was highly variable between different V. anguillarum lysogenic strains. NO16 prophages coexist with the V. anguillarum host in a mutualistic interaction by rendering fitness properties to the host, such as increased virulence and biofilm formation through lysogenic conversion, likely contributing to their global distribution.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Bacteriophages are the most abundant biological entities in the oceans and play key roles in bacterial activity, diversity and evolution. While extensive research has been conducted on the role of tailed viruses (Class: Caudoviricetes), very little is known about the distribution and functions of the non-tailed viruses (Class: Tectiliviricetes). The recent discovery of the lytic Autolykiviridae family demonstrated the potential importance of this structural lineage, emphasizing the need for further exploration of the role of this group of marine viruses. Here, we report the novel family of temperate phages under the class of Tectiliviricetes, which we propose to name “Asemoviridae” with phage NO16 as a main representative. These phages are widely distributed across geographical regions and isolation sources and found inside the genomes of at least 30 species of Vibrio, in addition to the original V. anguillarum isolation host. Genomic analysis identified dif-like sites, suggesting that NO16 prophages recombine with the bacterial genome based on the XerCD site-specific recombination mechanism. The interactions between the NO16 phage and its V. anguillarum host were linked to cell density and phage–host ratio. High cell density and low phage predation levels were shown to favor the temperate over the lytic lifestyle for NO16 viruses, and their spontaneous induction rate was highly variable between different V. anguillarum lysogenic strains. NO16 prophages coexist with the V. anguillarum host in a mutualistic interaction by rendering fitness properties to the host, such as increased virulence and biofilm formation through lysogenic conversion, likely contributing to their global distribution. |
Pavloudi, Christina; Tsertou, Maria Ioanna; Antonopoulou, Efthimia; Katharios, Pantelis Investigation of systemic granulomatosis in cultured meagre, Argyrosomus regius, using clinical metagenomics Journal Article Aquaculture, 567 , pp. 739249, 2023, ISSN: 00448486. @article{pavloudi_investigation_2023, title = {Investigation of systemic granulomatosis in cultured meagre, Argyrosomus regius, using clinical metagenomics}, author = {Christina Pavloudi and Maria Ioanna Tsertou and Efthimia Antonopoulou and Pantelis Katharios}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/01/2023-Pavloudi-AQUA-pre-print-8.pdf https://linkinghub.elsevier.com/retrieve/pii/S0044848623000224}, doi = {10.1016/j.aquaculture.2023.739249}, issn = {00448486}, year = {2023}, date = {2023-01-25}, urldate = {2023-01-25}, journal = {Aquaculture}, volume = {567}, pages = {739249}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2022 |
Katharios, Pantelis; Kokkari, Constantina; Cascarano, Maria Chiara; Gyparakis, Stylianos; Aggelidaki, Chrissi; Antonakaki, Ioanna; Vargiakaki, Efthimia A fish kill at the Aposelemis dam (Crete, Greece) caused by heavy parasitism by textitIchthyobodo sp. Journal Article Journal of Fish Diseases, pp. jfd.13717, 2022, ISSN: 0140-7775, 1365-2761. @article{katharios_fish_2022, title = {A fish kill at the Aposelemis dam (Crete, Greece) caused by heavy parasitism by textitIchthyobodo sp.}, author = {Pantelis Katharios and Constantina Kokkari and Maria Chiara Cascarano and Stylianos Gyparakis and Chrissi Aggelidaki and Ioanna Antonakaki and Efthimia Vargiakaki}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/09/2022-Katharios-Journal-of-Fish-Diseases-2022-Katharios-A-fish-kill-at-the-Aposelemis-dam-Crete-Greece-caused-by-heavy-parasitism-63.pdf https://onlinelibrary.wiley.com/doi/10.1111/jfd.13717}, doi = {10.1111/jfd.13717}, issn = {0140-7775, 1365-2761}, year = {2022}, date = {2022-09-01}, urldate = {2022-09-26}, journal = {Journal of Fish Diseases}, pages = {jfd.13717}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Droubogiannis, Stavros; Katharios, Pantelis Pathogens, 11 (6), pp. 630, 2022, ISSN: 2076-0817. @article{droubogiannis_genomic_2022, title = {Genomic and Biological Profile of a Novel Bacteriophage, Vibrio phage Virtus, Which Improves Survival of Sparus aurata Larvae Challenged with Vibrio harveyi}, author = {Stavros Droubogiannis and Pantelis Katharios}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/07/2022-Droubogiannis-Pathogens-46.pdf https://www.mdpi.com/2076-0817/11/6/630}, doi = {10.3390/pathogens11060630}, issn = {2076-0817}, year = {2022}, date = {2022-05-01}, urldate = {2022-07-29}, journal = {Pathogens}, volume = {11}, number = {6}, pages = {630}, abstract = {Due to the emergence of multidrug-resistant bacteria, commonly known as “superbugs”, phage therapy for the control of bacterial diseases rose in popularity. In this context, the use of phages for the management of many important bacterial diseases in the aquaculture environment is auspicious. Vibrio harveyi, a well-known and serious bacterial pathogen, is responsible for many disease outbreaks in aquaculture, resulting in huge economic and production losses. We isolated and fully characterized a novel bacteriophage, Vibrio phage Virtus, infecting V. harveyi strain VH2. Vibrio phage Virtus can infect a wide spectrum of Vibrio spp., including strains of V. harveyi, V. owensii, V. campbellii, V. parahaemolyticus, and V. mediterranei. It has a latent period of 40 min with an unusually high burst size of 3200 PFU/cell. Vibrio phage Virtus has a double-stranded DNA of 82,960 base pairs with 127 predicted open reading frames (ORFs). No virulence, antibiotic resistance, or integrase-encoding genes were detected. In vivo phage therapy trials in gilthead seabream, Sparus aurata, larvae demonstrated that Vibrio phage Virtus was able to significantly improve the survival of larvae for five days at a multiplicity of infection (MOI) of 10, which suggests that it can be an excellent candidate for phage therapy.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Due to the emergence of multidrug-resistant bacteria, commonly known as “superbugs”, phage therapy for the control of bacterial diseases rose in popularity. In this context, the use of phages for the management of many important bacterial diseases in the aquaculture environment is auspicious. Vibrio harveyi, a well-known and serious bacterial pathogen, is responsible for many disease outbreaks in aquaculture, resulting in huge economic and production losses. We isolated and fully characterized a novel bacteriophage, Vibrio phage Virtus, infecting V. harveyi strain VH2. Vibrio phage Virtus can infect a wide spectrum of Vibrio spp., including strains of V. harveyi, V. owensii, V. campbellii, V. parahaemolyticus, and V. mediterranei. It has a latent period of 40 min with an unusually high burst size of 3200 PFU/cell. Vibrio phage Virtus has a double-stranded DNA of 82,960 base pairs with 127 predicted open reading frames (ORFs). No virulence, antibiotic resistance, or integrase-encoding genes were detected. In vivo phage therapy trials in gilthead seabream, Sparus aurata, larvae demonstrated that Vibrio phage Virtus was able to significantly improve the survival of larvae for five days at a multiplicity of infection (MOI) of 10, which suggests that it can be an excellent candidate for phage therapy. |
Smyrli, Maria; Anka, Ishrat Zahan; Koutsoni, Olga; Dotsika, Eleni; Kyriazis, Ioannis D; Pavlidis, Michail; Katharios, Pantelis Development of autogenous vaccines for farmed European seabass against Aeromonas veronii using zebrafish as a model for efficacy assessment Journal Article Fish & Shellfish Immunology, 123 , pp. 381–387, 2022, ISSN: 10504648. @article{smyrli_development_2022, title = {Development of autogenous vaccines for farmed European seabass against Aeromonas veronii using zebrafish as a model for efficacy assessment}, author = {Maria Smyrli and Ishrat Zahan Anka and Olga Koutsoni and Eleni Dotsika and Ioannis D Kyriazis and Michail Pavlidis and Pantelis Katharios}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/05/2022-Smyrli-FSImmune-24-pre-print.pdf https://linkinghub.elsevier.com/retrieve/pii/S1050464822001450}, doi = {10.1016/j.fsi.2022.03.019}, issn = {10504648}, year = {2022}, date = {2022-04-01}, urldate = {2022-05-09}, journal = {Fish & Shellfish Immunology}, volume = {123}, pages = {381--387}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Cascarano, Maria Chiara; Ruetten, Maja; Vaughan, Lloyd; Tsertou, Maria Ioanna; Georgopoulou, Dimitra; Keklikoglou, Kleoniki; Papandroulakis, Nikos; Katharios, Pantelis Epitheliocystis in Greater Amberjack: Evidence of a Novel Causative Agent, Pathology, Immune Response and Epidemiological Findings Journal Article Microorganisms, 10 (3), pp. 627, 2022, ISSN: 2076-2607. @article{cascarano_epitheliocystis_2022, title = {Epitheliocystis in Greater Amberjack: Evidence of a Novel Causative Agent, Pathology, Immune Response and Epidemiological Findings}, author = {Maria Chiara Cascarano and Maja Ruetten and Lloyd Vaughan and Maria Ioanna Tsertou and Dimitra Georgopoulou and Kleoniki Keklikoglou and Nikos Papandroulakis and Pantelis Katharios}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/05/2022-Cascarano-Microorganisms-20.pdf https://www.mdpi.com/2076-2607/10/3/627}, doi = {10.3390/microorganisms10030627}, issn = {2076-2607}, year = {2022}, date = {2022-03-01}, urldate = {2022-05-09}, journal = {Microorganisms}, volume = {10}, number = {3}, pages = {627}, abstract = {Epitheliocystis is a fish gill disease caused by a broad range of intracellular bacteria infecting freshwater and marine fish worldwide. Here we report the occurrence and progression of epitheliocystis in greater amberjack reared in Crete (Greece). The disease appears to be caused mainly by a novel Betaproteobacteria belonging to the Candidatus Ichthyocystis genus with a second agent genetically similar to Ca. Parilichlamydia carangidicola coinfecting the gills in some cases. After a first detection of the disease in 2017, we investigated epitheliocystis in the following year’s cohort of greater amberjack juveniles (cohort 2018) transferred from inland tanks to the same cage farm in the open sea where the first outbreak was detected. This cohort was monitored for over a year together with stocks of gilthead seabream and meagre co-farmed in the same area. Our observations showed that epitheliocystis could be detected in greater amberjack gills as early as a month following the transfer to sea cages, with ionocytes at the base of the gill lamellae being initially infected. Cyst formation appears to trigger a proliferative response, leading to the fusion of lamellae, impairment of gill functions and subsequently to mortality. Lesions are characterized by infiltration of immune cells, indicating activation of the innate immune response. At later stages of the outbreak, cysts were no longer found in ionocytes but were observed in mucocytes at the trailing edge of the filament. Whole cysts appeared finally to be expelled from infected mucocytes directly into the water, which might constitute a novel means of dispersion of the infectious agents. Molecular screening indicates that meagre is not affected by this disease and confirms the presence of previously described epitheliocystis agents, Ca. Ichthyocystis sparus, Ca. Ichthyocystis hellenicum and Ca. Similichlamydia spp., in gilthead seabream. Prevalence data show that the bacteria persist in both gilthead seabream and greater amberjack cohorts after first infection.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Epitheliocystis is a fish gill disease caused by a broad range of intracellular bacteria infecting freshwater and marine fish worldwide. Here we report the occurrence and progression of epitheliocystis in greater amberjack reared in Crete (Greece). The disease appears to be caused mainly by a novel Betaproteobacteria belonging to the Candidatus Ichthyocystis genus with a second agent genetically similar to Ca. Parilichlamydia carangidicola coinfecting the gills in some cases. After a first detection of the disease in 2017, we investigated epitheliocystis in the following year’s cohort of greater amberjack juveniles (cohort 2018) transferred from inland tanks to the same cage farm in the open sea where the first outbreak was detected. This cohort was monitored for over a year together with stocks of gilthead seabream and meagre co-farmed in the same area. Our observations showed that epitheliocystis could be detected in greater amberjack gills as early as a month following the transfer to sea cages, with ionocytes at the base of the gill lamellae being initially infected. Cyst formation appears to trigger a proliferative response, leading to the fusion of lamellae, impairment of gill functions and subsequently to mortality. Lesions are characterized by infiltration of immune cells, indicating activation of the innate immune response. At later stages of the outbreak, cysts were no longer found in ionocytes but were observed in mucocytes at the trailing edge of the filament. Whole cysts appeared finally to be expelled from infected mucocytes directly into the water, which might constitute a novel means of dispersion of the infectious agents. Molecular screening indicates that meagre is not affected by this disease and confirms the presence of previously described epitheliocystis agents, Ca. Ichthyocystis sparus, Ca. Ichthyocystis hellenicum and Ca. Similichlamydia spp., in gilthead seabream. Prevalence data show that the bacteria persist in both gilthead seabream and greater amberjack cohorts after first infection. |
Grau, Amalia; Villalba, Antonio; Navas, José I; Hansjosten, Beatriz; Valencia, José M; García-March, José R; Prado, Patricia; Follana-Berná, Guillermo; Morage, Titouan; Vázquez-Luis, Maite; Álvarez, Elvira; Katharios, Pantelis; Pavloudi, Christina; Nebot-Colomer, Elisabet; Tena-Medialdea, José; Lopez-Sanmartín, Monserrat; Peyran, Claire; Čižmek, Hrvoje; Sarafidou, Georgia; Issaris, Yiannis; Tüney-Kizilkaya, Inci; Deudero, Salud; Planes, Serge; Catanese, Gaetano Wide-Geographic and Long-Term Analysis of the Role of Pathogens in the Decline of Pinna nobilis to Critically Endangered Species Journal Article Frontiers in Marine Science, 9 , pp. 666640, 2022, ISSN: 2296-7745. @article{grau_wide-geographic_2022, title = {Wide-Geographic and Long-Term Analysis of the Role of Pathogens in the Decline of Pinna nobilis to Critically Endangered Species}, author = {Amalia Grau and Antonio Villalba and José I Navas and Beatriz Hansjosten and José M Valencia and José R García-March and Patricia Prado and Guillermo Follana-Berná and Titouan Morage and Maite Vázquez-Luis and Elvira Álvarez and Pantelis Katharios and Christina Pavloudi and Elisabet Nebot-Colomer and José Tena-Medialdea and Monserrat Lopez-Sanmartín and Claire Peyran and Hrvoje Čižmek and Georgia Sarafidou and Yiannis Issaris and Inci Tüney-Kizilkaya and Salud Deudero and Serge Planes and Gaetano Catanese}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/05/2022-Grau-FMSci-21.pdf https://www.frontiersin.org/articles/10.3389/fmars.2022.666640/full}, doi = {10.3389/fmars.2022.666640}, issn = {2296-7745}, year = {2022}, date = {2022-03-01}, urldate = {2022-05-09}, journal = {Frontiers in Marine Science}, volume = {9}, pages = {666640}, abstract = {A mass mortality event (MME) affecting the fan mussel Pinna nobilis was first detected in Spain in autumn 2016 and spread north- and eastward through the Mediterranean Sea. Various pathogens have been blamed for contributing to the MME, with emphasis in Haplosporidium pinnae , Mycobacterium sp. and Vibrio spp. In this study, samples from 762 fan mussels (necropsies from 263 individuals, mantle biopsies from 499) of various health conditions, with wide geographic and age range, taken before and during the MME spread from various environments along Mediterranean Sea, were used to assess the role of pathogens in the MME. The number of samples processed by both histological and molecular methods was 83. The most important factor playing a main role on the onset of the mass mortality of P. nobilis throughout the Mediterranean Sea was the infection by H. pinnae . It was the only non-detected pathogen before the MME while, during MME spreading, its prevalence was higher in sick and dead individuals than in asymptomatic ones, in MME-affected areas than in non-affected sites, and it was not associated with host size, infecting both juveniles and adults. Conversely, infection with mycobacteria was independent from the period (before or during MME), from the affection of the area by MME and from the host health condition, and it was associated with host size. Gram (-) bacteria neither appeared associated with MME.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A mass mortality event (MME) affecting the fan mussel Pinna nobilis was first detected in Spain in autumn 2016 and spread north- and eastward through the Mediterranean Sea. Various pathogens have been blamed for contributing to the MME, with emphasis in Haplosporidium pinnae , Mycobacterium sp. and Vibrio spp. In this study, samples from 762 fan mussels (necropsies from 263 individuals, mantle biopsies from 499) of various health conditions, with wide geographic and age range, taken before and during the MME spread from various environments along Mediterranean Sea, were used to assess the role of pathogens in the MME. The number of samples processed by both histological and molecular methods was 83. The most important factor playing a main role on the onset of the mass mortality of P. nobilis throughout the Mediterranean Sea was the infection by H. pinnae . It was the only non-detected pathogen before the MME while, during MME spreading, its prevalence was higher in sick and dead individuals than in asymptomatic ones, in MME-affected areas than in non-affected sites, and it was not associated with host size, infecting both juveniles and adults. Conversely, infection with mycobacteria was independent from the period (before or during MME), from the affection of the area by MME and from the host health condition, and it was associated with host size. Gram (-) bacteria neither appeared associated with MME. |
Cascarano, Maria Chiara; Katharios, Pantelis Expansion of the Beta-Proteobacterial Genus Ca. Ichthyocystis: A Case Report of Epitheliocystis in the Pompano Trachinotus ovatus Journal Article Pathogens, 11 (4), pp. 421, 2022, ISSN: 2076-0817. @article{cascarano_expansion_2022, title = {Expansion of the Beta-Proteobacterial Genus Ca. Ichthyocystis: A Case Report of Epitheliocystis in the Pompano Trachinotus ovatus}, author = {Maria Chiara Cascarano and Pantelis Katharios}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/05/2022-Cascarano-Pathogens-28.pdf https://www.mdpi.com/2076-0817/11/4/421}, doi = {10.3390/pathogens11040421}, issn = {2076-0817}, year = {2022}, date = {2022-03-01}, urldate = {2022-05-09}, journal = {Pathogens}, volume = {11}, number = {4}, pages = {421}, abstract = {Epitheliocystis is a disease caused by a wide variety of host-specific intracellular bacteria infecting fish gills. In the Mediterranean Sea, epitheliocystis has been recently associated with a novel genus of beta-proteobacteria, the Ca. Ichthyocystis genus. In the present study, we report a case of epitheliocystis in a wild-caught specimen of pompano Trachinotus ovatus in Crete, Greece. Molecular analysis of partial 16s rRNA sequence led to the discovery of a putative novel species of the Ca. Ichthyocystis genus. Investigation of the phylogenetic relationship between closely related sequences deposited in NCBI suggests that bacterial ancestors in gilthead seabream might have a pivotal role in the differentiation of genus.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Epitheliocystis is a disease caused by a wide variety of host-specific intracellular bacteria infecting fish gills. In the Mediterranean Sea, epitheliocystis has been recently associated with a novel genus of beta-proteobacteria, the Ca. Ichthyocystis genus. In the present study, we report a case of epitheliocystis in a wild-caught specimen of pompano Trachinotus ovatus in Crete, Greece. Molecular analysis of partial 16s rRNA sequence led to the discovery of a putative novel species of the Ca. Ichthyocystis genus. Investigation of the phylogenetic relationship between closely related sequences deposited in NCBI suggests that bacterial ancestors in gilthead seabream might have a pivotal role in the differentiation of genus. |
Tsertou, M I; Papandroulakis, N; Keklikoglou, K; Kalantzi, I; Tsapakis, M; Tsalafouta, A; Pavlidis, M; Antonopoulou, E; Katharios, P Comparative study of Chronic Ulcerative Dermatopathy in cultured meagre, Argyrosomus regius Journal Article Aquaculture, 556 , pp. 738301, 2022, ISSN: 00448486. @article{tsertou_comparative_2022, title = {Comparative study of Chronic Ulcerative Dermatopathy in cultured meagre, Argyrosomus regius}, author = {M I Tsertou and N Papandroulakis and K Keklikoglou and I Kalantzi and M Tsapakis and A Tsalafouta and M Pavlidis and E Antonopoulou and P Katharios}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/05/2022-Tsertou-AQUA-pre-print-34.pdf https://linkinghub.elsevier.com/retrieve/pii/S0044848622004173}, doi = {10.1016/j.aquaculture.2022.738301}, issn = {00448486}, year = {2022}, date = {2022-01-01}, urldate = {2022-05-09}, journal = {Aquaculture}, volume = {556}, pages = {738301}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Droubogiannis, Stavros; Pavlidi, Lydia; Tsertou, Maria Ioanna; Kokkari, Constantina; Skliros, Dimitrios; Flemetakis, Emmanouil; Katharios, Pantelis Vibrio Phage Artemius, a Novel Phage Infecting Vibrio alginolyticus Journal Article Pathogens, 11 (8), pp. 848, 2022, ISSN: 2076-0817. @article{droubogiannis_vibrio_2022, title = {Vibrio Phage Artemius, a Novel Phage Infecting Vibrio alginolyticus}, author = {Stavros Droubogiannis and Lydia Pavlidi and Maria Ioanna Tsertou and Constantina Kokkari and Dimitrios Skliros and Emmanouil Flemetakis and Pantelis Katharios}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/07/2022-Droubogiannis-pathogen-54.pdf https://www.mdpi.com/2076-0817/11/8/848}, doi = {10.3390/pathogens11080848}, issn = {2076-0817}, year = {2022}, date = {2022-01-01}, urldate = {2022-07-29}, journal = {Pathogens}, volume = {11}, number = {8}, pages = {848}, abstract = {Vibrio alginolyticus is an important pathogen of marine animals and has been the target of phage therapy applications in marine aquaculture for many years. Here, we report the isolation and partial characterization of a novel species of the Siphoviridae family, the Vibrio phage Artemius. The novel phage was species-specific and could only infect strains of V. alginolyticus. It could efficiently reduce the growth of the host bacterium at various multiplicities of infection as assessed by an in vitro lysis assay. It had a genome length of 43,349 base pairs. The complete genome has double-stranded DNA with a G + C content of 43.61%. In total, 57 ORFs were identified, of which 19 were assigned a predicted function. A genomic analysis indicated that Vibrio phage Artemius is lytic and does not harbor genes encoding toxins and antibiotic resistance determinants.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Vibrio alginolyticus is an important pathogen of marine animals and has been the target of phage therapy applications in marine aquaculture for many years. Here, we report the isolation and partial characterization of a novel species of the Siphoviridae family, the Vibrio phage Artemius. The novel phage was species-specific and could only infect strains of V. alginolyticus. It could efficiently reduce the growth of the host bacterium at various multiplicities of infection as assessed by an in vitro lysis assay. It had a genome length of 43,349 base pairs. The complete genome has double-stranded DNA with a G + C content of 43.61%. In total, 57 ORFs were identified, of which 19 were assigned a predicted function. A genomic analysis indicated that Vibrio phage Artemius is lytic and does not harbor genes encoding toxins and antibiotic resistance determinants. |
Tsertou, Maria Ioanna; Antonopoulou, Efthimia; Katharios, Pantelis Chronic diseases of the lateral line organ in fish Incollection Aquaculture Pathophysiology, pp. 721–725, Elsevier, 2022, ISBN: 9780128122112. @incollection{tsertou_chronic_2022, title = {Chronic diseases of the lateral line organ in fish}, author = {Maria Ioanna Tsertou and Efthimia Antonopoulou and Pantelis Katharios}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/10/2022-Tsertou-EUD-preprint-67-1.pdf https://linkinghub.elsevier.com/retrieve/pii/B9780128122112000640}, doi = {10.1016/B978-0-12-812211-2.00064-0}, isbn = {9780128122112}, year = {2022}, date = {2022-01-01}, urldate = {2022-10-12}, booktitle = {Aquaculture Pathophysiology}, pages = {721--725}, publisher = {Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {incollection} } |
Triga, Adriana; Smyrli, Maria; Katharios, Pantelis Aeromoniasis Incollection Aquaculture Pathophysiology, pp. 317–327, Elsevier, 2022, ISBN: 9780128122112. @incollection{triga_aeromoniasis_2022, title = {Aeromoniasis}, author = {Adriana Triga and Maria Smyrli and Pantelis Katharios}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/10/2022-Tringa-chapter-25-Aeromonas-preprint-68.pdf https://linkinghub.elsevier.com/retrieve/pii/B978012812211200024X}, doi = {10.1016/B978-0-12-812211-2.00024-X}, isbn = {9780128122112}, year = {2022}, date = {2022-01-01}, urldate = {2022-10-12}, booktitle = {Aquaculture Pathophysiology}, pages = {317--327}, publisher = {Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {incollection} } |
2021 |
Papadaki, Maria; Mandalakis, Manolis; Anastasiou, Thekla I; Pouli, Marina; Asderis, Michalis; Katharios, Pantelis; Papandroulakis, Nikos; Mylonas, Constantinos C Fish Physiology and Biochemistry, 47 (6), pp. 1777–1792, 2021, ISSN: 1573-5168. @article{papadaki_histological_2021b, title = {Histological evaluation of sex differentiation and early sex identification in hatchery-produced greater amberjack (Seriola dumerili) reared in sea cages}, author = {Maria Papadaki and Manolis Mandalakis and Thekla I Anastasiou and Marina Pouli and Michalis Asderis and Pantelis Katharios and Nikos Papandroulakis and Constantinos C Mylonas}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/01/2021-Papadaki-FFB-82-pre-print.pdf https://doi.org/10.1007/s10695-021-01007-7}, doi = {10.1007/s10695-021-01007-7}, issn = {1573-5168}, year = {2021}, date = {2021-12-01}, urldate = {2021-12-04}, journal = {Fish Physiology and Biochemistry}, volume = {47}, number = {6}, pages = {1777--1792}, abstract = {The histological process of gonadal differentiation, together with the endocrine changes of sex steroid hormones and some of their precursors, was studied in hatchery-produced greater amberjack Seriola dumerili from 101 until 408 days post-hatching (dph), with samplings conducted every 50 days. Histological processing showed that sex differentiation began at 101 dph with the formation of the ovarian cavity in females, while the presumptive males did not yet contain any germ cells in their gonad. At 150 dph, we observed the first germ cells in the developing testes. Sex differentiation in almost all sampled individuals was complete at 408 dph. No size dimorphism was observed between the sexes, and the sex ratio was 1:1, suggesting that there was no influence of early rearing in captivity on sex differentiation. Plasma concentrations of adrenosterone (Ad), androstenedione (Δ4), 11-ketotestosterone (11ΚΤ), testosterone (Τ), estradiol (Ε2), progesterone (P4) and 17,20β-dihydroxy-4-pregnen-3-one (17,20βP) were measured in males and females with the use of liquid chromatography tandem mass spectrometry (LC–MS/MS) to examine their role in the sex differentiation process. From the seven hormones, the only one that exhibited differences between the sexes was 11-KT and the plasma 11-KT concentration was found to be a useful indication of greater amberjack sex. Variations were observed in the mean values of Ad, Δ4, 11-KT, T, P4 and 17,20βP over time in one or both sexes, indicating their involvement in the sex differentiation process.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The histological process of gonadal differentiation, together with the endocrine changes of sex steroid hormones and some of their precursors, was studied in hatchery-produced greater amberjack Seriola dumerili from 101 until 408 days post-hatching (dph), with samplings conducted every 50 days. Histological processing showed that sex differentiation began at 101 dph with the formation of the ovarian cavity in females, while the presumptive males did not yet contain any germ cells in their gonad. At 150 dph, we observed the first germ cells in the developing testes. Sex differentiation in almost all sampled individuals was complete at 408 dph. No size dimorphism was observed between the sexes, and the sex ratio was 1:1, suggesting that there was no influence of early rearing in captivity on sex differentiation. Plasma concentrations of adrenosterone (Ad), androstenedione (Δ4), 11-ketotestosterone (11ΚΤ), testosterone (Τ), estradiol (Ε2), progesterone (P4) and 17,20β-dihydroxy-4-pregnen-3-one (17,20βP) were measured in males and females with the use of liquid chromatography tandem mass spectrometry (LC–MS/MS) to examine their role in the sex differentiation process. From the seven hormones, the only one that exhibited differences between the sexes was 11-KT and the plasma 11-KT concentration was found to be a useful indication of greater amberjack sex. Variations were observed in the mean values of Ad, Δ4, 11-KT, T, P4 and 17,20βP over time in one or both sexes, indicating their involvement in the sex differentiation process. |
Cascarano, Maria Chiara; Stavrakidis-Zachou, Orestis; Mladineo, Ivona; Thompson, Kim D; Papandroulakis, Nikos; Katharios, Pantelis Mediterranean Aquaculture in a Changing Climate: Temperature Effects on Pathogens and Diseases of Three Farmed Fish Species Journal Article 10 (9), pp. 1205, 2021. @article{cascarano_mediterranean_2021, title = {Mediterranean Aquaculture in a Changing Climate: Temperature Effects on Pathogens and Diseases of Three Farmed Fish Species}, author = {Maria Chiara Cascarano and Orestis Stavrakidis-Zachou and Ivona Mladineo and Kim D Thompson and Nikos Papandroulakis and Pantelis Katharios}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/09/2021-Cascarano-pathogens-71.pdf https://www.mdpi.com/2076-0817/10/9/1205}, doi = {10.3390/pathogens10091205}, year = {2021}, date = {2021-09-01}, urldate = {2021-09-22}, volume = {10}, number = {9}, pages = {1205}, abstract = {Climate change is expected to have a drastic effect on aquaculture worldwide. As we move forward with the agenda to increase and diversify aquaculture production, rising temperatures will have a progressively relevant impact on fish farming, linked to a multitude of issues associated with fish welfare. Temperature affects the physiology of both fish and pathogens, and has the potential to lead to significant increases in disease outbreaks within aquaculture systems, resulting in severe financial impacts. Significant shifts in future temperature regimes are projected for the Mediterranean Sea. We therefore aim to review and discuss the existing knowledge relating to disease outbreaks in the context of climate change in Mediterranean finfish aquaculture. The objective is to describe the effects of temperature on the physiology of both fish and pathogens, and moreover to list and discuss the principal diseases of the three main fish species farmed in the Mediterranean, namely gilthead seabream (Sparus aurata), European seabass (Dicentrarchus labrax), and meagre (Argyrosomus regius). We will attempt to link the pathology of each disease to a specific temperature range, while discussing potential future disease threats associated with the available climate change trends for the Mediterranean Sea.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Climate change is expected to have a drastic effect on aquaculture worldwide. As we move forward with the agenda to increase and diversify aquaculture production, rising temperatures will have a progressively relevant impact on fish farming, linked to a multitude of issues associated with fish welfare. Temperature affects the physiology of both fish and pathogens, and has the potential to lead to significant increases in disease outbreaks within aquaculture systems, resulting in severe financial impacts. Significant shifts in future temperature regimes are projected for the Mediterranean Sea. We therefore aim to review and discuss the existing knowledge relating to disease outbreaks in the context of climate change in Mediterranean finfish aquaculture. The objective is to describe the effects of temperature on the physiology of both fish and pathogens, and moreover to list and discuss the principal diseases of the three main fish species farmed in the Mediterranean, namely gilthead seabream (Sparus aurata), European seabass (Dicentrarchus labrax), and meagre (Argyrosomus regius). We will attempt to link the pathology of each disease to a specific temperature range, while discussing potential future disease threats associated with the available climate change trends for the Mediterranean Sea. |
Kogiannou, Dimitra; Nikoloudaki, Chrysanthi; Katharios, Pantelis; Triga, Adriana; Rigos, George Evaluation of absorption and depletion of florfenicol in European seabass textitDicentrarchus labrax Journal Article Veterinary Medicine and Science, 7 (3), pp. 987–997, 2021, ISSN: 2053-1095, 2053-1095. @article{kogiannou_evaluation_2021, title = {Evaluation of absorption and depletion of florfenicol in European seabass textitDicentrarchus labrax}, author = {Dimitra Kogiannou and Chrysanthi Nikoloudaki and Pantelis Katharios and Adriana Triga and George Rigos}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/06/2021-Kogiannou-VetMed-Sci-40.pdf https://onlinelibrary.wiley.com/doi/10.1002/vms3.415}, doi = {10.1002/vms3.415}, issn = {2053-1095, 2053-1095}, year = {2021}, date = {2021-05-01}, urldate = {2021-06-24}, journal = {Veterinary Medicine and Science}, volume = {7}, number = {3}, pages = {987--997}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Skliros, Dimitrios; Kalatzis, Panos G; Kalloniati, Chrysanthi; Komaitis, Fotios; Papathanasiou, Sokratis; Kouri, Evangelia D; Udvardi, Michael K; Kokkari, Constantina; Katharios, Pantelis; Flemetakis, Emmanouil The Development of Bacteriophage Resistance in Vibrio alginolyticus Depends on a Complex Metabolic Adaptation Strategy Journal Article Viruses, 13 (4), pp. 656, 2021. @article{skliros_development_2021, title = {The Development of Bacteriophage Resistance in Vibrio alginolyticus Depends on a Complex Metabolic Adaptation Strategy}, author = {Dimitrios Skliros and Panos G Kalatzis and Chrysanthi Kalloniati and Fotios Komaitis and Sokratis Papathanasiou and Evangelia D Kouri and Michael K Udvardi and Constantina Kokkari and Pantelis Katharios and Emmanouil Flemetakis}, url = {https://www.mdpi.com/1999-4915/13/4/656 https://imbbc.hcmr.gr/wp-content/uploads/2021/05/2021-Sκliros-viruses-29-1.pdf}, doi = {10.3390/v13040656}, year = {2021}, date = {2021-04-01}, urldate = {2021-04-27}, journal = {Viruses}, volume = {13}, number = {4}, pages = {656}, abstract = {Lytic bacteriophages have been well documented to play a pivotal role in microbial ecology due to their complex interactions with bacterial species, especially in aquatic habitats. Although the use of phages as antimicrobial agents, known as phage therapy, in the aquatic environment has been increasing, recent research has revealed drawbacks due to the development of phage-resistant strains among Gram-negative species. Acquired phage resistance in marine Vibrios has been proven to be a very complicated process utilizing biochemical, metabolic, and molecular adaptation strategies. The results of our multi-omics approach, incorporating transcriptome and metabolome analyses of Vibrio alginolyticus phage-resistant strains, corroborate this prospect. Our results provide insights into phage-tolerant strains diminishing the expression of phage receptors ompF, lamB, and btuB. The same pattern was observed for genes encoding natural nutrient channels, such as rbsA, ptsG, tryP, livH, lysE, and hisp, meaning that the cell needs to readjust its biochemistry to achieve phage resistance. The results showed reprogramming of bacterial metabolism by transcript regulations in key-metabolic pathways, such as the tricarboxylic acid cycle (TCA) and lysine biosynthesis, as well as the content of intracellular metabolites belonging to processes that could also significantly affect the cell physiology. Finally, SNP analysis in resistant strains revealed no evidence of amino acid alterations in the studied putative bacterial phage receptors, but several SNPs were detected in genes involved in transcriptional regulation. This phenomenon appears to be a phage-specific, fine-tuned metabolic engineering, imposed by the different phage genera the bacteria have interacted with, updating the role of lytic phages in microbial marine ecology.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Lytic bacteriophages have been well documented to play a pivotal role in microbial ecology due to their complex interactions with bacterial species, especially in aquatic habitats. Although the use of phages as antimicrobial agents, known as phage therapy, in the aquatic environment has been increasing, recent research has revealed drawbacks due to the development of phage-resistant strains among Gram-negative species. Acquired phage resistance in marine Vibrios has been proven to be a very complicated process utilizing biochemical, metabolic, and molecular adaptation strategies. The results of our multi-omics approach, incorporating transcriptome and metabolome analyses of Vibrio alginolyticus phage-resistant strains, corroborate this prospect. Our results provide insights into phage-tolerant strains diminishing the expression of phage receptors ompF, lamB, and btuB. The same pattern was observed for genes encoding natural nutrient channels, such as rbsA, ptsG, tryP, livH, lysE, and hisp, meaning that the cell needs to readjust its biochemistry to achieve phage resistance. The results showed reprogramming of bacterial metabolism by transcript regulations in key-metabolic pathways, such as the tricarboxylic acid cycle (TCA) and lysine biosynthesis, as well as the content of intracellular metabolites belonging to processes that could also significantly affect the cell physiology. Finally, SNP analysis in resistant strains revealed no evidence of amino acid alterations in the studied putative bacterial phage receptors, but several SNPs were detected in genes involved in transcriptional regulation. This phenomenon appears to be a phage-specific, fine-tuned metabolic engineering, imposed by the different phage genera the bacteria have interacted with, updating the role of lytic phages in microbial marine ecology. |
Mandalakis, Manolis; Anastasiou, Thekla I; Martou, Natalia; Keisaris, Sofoklis; Greveniotis, Vasileios; Katharios, Pantelis; Lazari, Diamanto; Krigas, Nikos; Antonopoulou, Efthimia Molecules, 26 (9), pp. 2731, 2021. @article{mandalakis_antibacterial_2021, title = {Antibacterial Effects of Essential Oils of Seven Medicinal-Aromatic Plants Against the Fish Pathogen Aeromonas veronii bv. sobria: To Blend or Not to Blend?}, author = {Manolis Mandalakis and Thekla I Anastasiou and Natalia Martou and Sofoklis Keisaris and Vasileios Greveniotis and Pantelis Katharios and Diamanto Lazari and Nikos Krigas and Efthimia Antonopoulou}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/05/2021-Mandalakis-molecules-37.pdf https://www.mdpi.com/1420-3049/26/9/2731}, doi = {10.3390/molecules26092731}, year = {2021}, date = {2021-01-01}, urldate = {2021-05-10}, journal = {Molecules}, volume = {26}, number = {9}, pages = {2731}, abstract = {Despite progress achieved, there is limited available information about the antibacterial activity of constituents of essential oils (EOs) from different medicinal-aromatic plants (MAPs) against fish pathogens and the complex interactions of blended EOs thereof. The present study aimed to investigate possible synergistic antimicrobial effects of EOs from seven Greek MAPs with strong potential against Aeromonas veronii bv. sobria, a fish pathogen associated with aquaculture disease outbreaks. The main objective was to evaluate whether blending of these EOs can lead to increased antimicrobial activity against the specific microorganism. A total of 127 combinations of EOs were prepared and their effect on A. veronii bv. sobria growth was tested in vitro. We examined both the inhibitory and bactericidal activities of the individual EOs and compared them to those of the blended EOs. The vast majority of the investigated combinations exhibited significant synergistic and additive effects, while antagonistic effects were evident only in a few cases, such as the mixtures containing EOs from rosemary, lemon balm and pennyroyal. The combination of EOs from Greek oregano and wild carrot, as well as the combinations of those two with Spanish oregano or savoury were the most promising ones. Overall, Greek oregano, savoury and Spanish oregano EOs were the most effective ones when applied either in pure form or blended with other EOs.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Despite progress achieved, there is limited available information about the antibacterial activity of constituents of essential oils (EOs) from different medicinal-aromatic plants (MAPs) against fish pathogens and the complex interactions of blended EOs thereof. The present study aimed to investigate possible synergistic antimicrobial effects of EOs from seven Greek MAPs with strong potential against Aeromonas veronii bv. sobria, a fish pathogen associated with aquaculture disease outbreaks. The main objective was to evaluate whether blending of these EOs can lead to increased antimicrobial activity against the specific microorganism. A total of 127 combinations of EOs were prepared and their effect on A. veronii bv. sobria growth was tested in vitro. We examined both the inhibitory and bactericidal activities of the individual EOs and compared them to those of the blended EOs. The vast majority of the investigated combinations exhibited significant synergistic and additive effects, while antagonistic effects were evident only in a few cases, such as the mixtures containing EOs from rosemary, lemon balm and pennyroyal. The combination of EOs from Greek oregano and wild carrot, as well as the combinations of those two with Spanish oregano or savoury were the most promising ones. Overall, Greek oregano, savoury and Spanish oregano EOs were the most effective ones when applied either in pure form or blended with other EOs. |
Papadaki, Maria; Mandalakis, Manolis; Anastasiou, Thekla I; Pouli, Marina; Asderis, Michalis; Katharios, Pantelis; Papandroulakis, Nikos; Mylonas, Constantinos C Fish Physiology and Biochemistry, 47 (6), pp. 1777–1792, 2021, ISSN: 1573-5168. @article{papadaki_histological_2021, title = {Histological evaluation of sex differentiation and early sex identification in hatchery-produced greater amberjack (Seriola dumerili) reared in sea cages}, author = {Maria Papadaki and Manolis Mandalakis and Thekla I Anastasiou and Marina Pouli and Michalis Asderis and Pantelis Katharios and Nikos Papandroulakis and Constantinos C Mylonas}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/12/2021-Papadaki-FFB-69-pre-print-1.pdf https://doi.org/10.1007/s10695-021-01007-7}, doi = {10.1007/s10695-021-01007-7}, issn = {1573-5168}, year = {2021}, date = {2021-01-01}, urldate = {2021-12-04}, journal = {Fish Physiology and Biochemistry}, volume = {47}, number = {6}, pages = {1777--1792}, abstract = {The histological process of gonadal differentiation, together with the endocrine changes of sex steroid hormones and some of their precursors, was studied in hatchery-produced greater amberjack Seriola dumerili from 101 until 408 days post-hatching (dph), with samplings conducted every 50 days. Histological processing showed that sex differentiation began at 101 dph with the formation of the ovarian cavity in females, while the presumptive males did not yet contain any germ cells in their gonad. At 150 dph, we observed the first germ cells in the developing testes. Sex differentiation in almost all sampled individuals was complete at 408 dph. No size dimorphism was observed between the sexes, and the sex ratio was 1:1, suggesting that there was no influence of early rearing in captivity on sex differentiation. Plasma concentrations of adrenosterone (Ad), androstenedione (Δ4), 11-ketotestosterone (11ΚΤ), testosterone (Τ), estradiol (Ε2), progesterone (P4) and 17,20β-dihydroxy-4-pregnen-3-one (17,20βP) were measured in males and females with the use of liquid chromatography tandem mass spectrometry (LC–MS/MS) to examine their role in the sex differentiation process. From the seven hormones, the only one that exhibited differences between the sexes was 11-KT and the plasma 11-KT concentration was found to be a useful indication of greater amberjack sex. Variations were observed in the mean values of Ad, Δ4, 11-KT, T, P4 and 17,20βP over time in one or both sexes, indicating their involvement in the sex differentiation process.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The histological process of gonadal differentiation, together with the endocrine changes of sex steroid hormones and some of their precursors, was studied in hatchery-produced greater amberjack Seriola dumerili from 101 until 408 days post-hatching (dph), with samplings conducted every 50 days. Histological processing showed that sex differentiation began at 101 dph with the formation of the ovarian cavity in females, while the presumptive males did not yet contain any germ cells in their gonad. At 150 dph, we observed the first germ cells in the developing testes. Sex differentiation in almost all sampled individuals was complete at 408 dph. No size dimorphism was observed between the sexes, and the sex ratio was 1:1, suggesting that there was no influence of early rearing in captivity on sex differentiation. Plasma concentrations of adrenosterone (Ad), androstenedione (Δ4), 11-ketotestosterone (11ΚΤ), testosterone (Τ), estradiol (Ε2), progesterone (P4) and 17,20β-dihydroxy-4-pregnen-3-one (17,20βP) were measured in males and females with the use of liquid chromatography tandem mass spectrometry (LC–MS/MS) to examine their role in the sex differentiation process. From the seven hormones, the only one that exhibited differences between the sexes was 11-KT and the plasma 11-KT concentration was found to be a useful indication of greater amberjack sex. Variations were observed in the mean values of Ad, Δ4, 11-KT, T, P4 and 17,20βP over time in one or both sexes, indicating their involvement in the sex differentiation process. |
2020 |
Misol, Gerald N; Kokkari, Constantinta; Katharios, Pantelis Complete Genome Sequence of a Jumbo Bacteriophage, vB_pir03, against textitVibrio harveyi Journal Article Microbiology Resource Announcements, 9 (44), pp. e00910–20, /mra/9/44/MRA.00910–20.atom, 2020, ISSN: 2576-098X. @article{misol_complete_2020, title = {Complete Genome Sequence of a Jumbo Bacteriophage, vB_pir03, against textitVibrio harveyi}, author = {Gerald N Misol and Constantinta Kokkari and Pantelis Katharios}, editor = {John J Dennehy}, url = {https://mra.asm.org/content/9/44/e00910-20 https://imbbc.hcmr.gr/wp-content/uploads/2020/12/Katharios-2020-Microbiology-Resource-Announcements.pdf}, doi = {10.1128/MRA.00910-20}, issn = {2576-098X}, year = {2020}, date = {2020-10-01}, urldate = {2020-12-08}, journal = {Microbiology Resource Announcements}, volume = {9}, number = {44}, pages = {e00910--20, /mra/9/44/MRA.00910--20.atom}, abstract = {ABSTRACT Vibrio harveyi is a persistent pathogen responsible for disease outbreaks in aquaculture. We have sequenced the genome of a jumbo Vibrio phage, vB_pir03, isolated in Greece. Here, we present the complete genome of vB_pir03, which consists of 286,284 bp and 336 open reading frames.}, keywords = {}, pubstate = {published}, tppubtype = {article} } ABSTRACT Vibrio harveyi is a persistent pathogen responsible for disease outbreaks in aquaculture. We have sequenced the genome of a jumbo Vibrio phage, vB_pir03, isolated in Greece. Here, we present the complete genome of vB_pir03, which consists of 286,284 bp and 336 open reading frames. |
Rigos, George; Katharios, Pantelis; Kogiannou, Dimitra; Cascarano, Chiara M Infectious diseases and treatment solutions of farmed greater amberjack Seriola dumerili with particular emphasis in Mediterranean region Journal Article Reviews in Aquaculture, pp. raq.12476, 2020, ISSN: 1753-5123, 1753-5131. @article{rigos_infectious_2020, title = {Infectious diseases and treatment solutions of farmed greater amberjack Seriola dumerili with particular emphasis in Mediterranean region}, author = {George Rigos and Pantelis Katharios and Dimitra Kogiannou and Chiara M Cascarano}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/raq.12476}, doi = {10.1111/raq.12476}, issn = {1753-5123, 1753-5131}, year = {2020}, date = {2020-07-01}, urldate = {2020-09-02}, journal = {Reviews in Aquaculture}, pages = {raq.12476}, abstract = {Abstract Greater amberjack (Seriola dumerili) is a very promising candidate for the diversification of the Mediterranean aquaculture due to its high demand, excellent flesh quality and high market prices. Its production expansion has, however, failed so far, due to several bottlenecks mainly related to pathology. This review addresses the major pathogens, which hinder the culture of greater amberjack with special focus in the Mediterranean region, and highlights possible treatment solutions. Among the important recorded pathogens of caged greater amberjack in the Mediterranean, the gill monogenean Zeuxapta seriolae seems to be the most problematic, causing significant losses. While formalin immersions are inefficient to combat this parasite, baths with hydrogen peroxide are extremely effective and praziquantel administration could be a more practical in-feed treatment solution. The digenean blood flukes, Paradeontacylix spp., also account for important losses in greater amberjack farms in the same region. Dietary administration of praziquantel constitutes an effective therapeutic measure against those infections. Vibrio harveyi is also a bacterial pathogen severely affecting fish maintained both in land-based facilities and in cages, whereas Epitheliocystis is a disease reported frequently that can be fatal when it occurs at early stages. Skin flukes such as Benedenia seriolae and Neobenedenia girellae as well as other parasites bacteria and viruses mentioned herein, which have caused substantial losses in Asian enterprises, but have not been identified yet in greater amberjack farmed in the Mediterranean, should be considered as potential threats.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Greater amberjack (Seriola dumerili) is a very promising candidate for the diversification of the Mediterranean aquaculture due to its high demand, excellent flesh quality and high market prices. Its production expansion has, however, failed so far, due to several bottlenecks mainly related to pathology. This review addresses the major pathogens, which hinder the culture of greater amberjack with special focus in the Mediterranean region, and highlights possible treatment solutions. Among the important recorded pathogens of caged greater amberjack in the Mediterranean, the gill monogenean Zeuxapta seriolae seems to be the most problematic, causing significant losses. While formalin immersions are inefficient to combat this parasite, baths with hydrogen peroxide are extremely effective and praziquantel administration could be a more practical in-feed treatment solution. The digenean blood flukes, Paradeontacylix spp., also account for important losses in greater amberjack farms in the same region. Dietary administration of praziquantel constitutes an effective therapeutic measure against those infections. Vibrio harveyi is also a bacterial pathogen severely affecting fish maintained both in land-based facilities and in cages, whereas Epitheliocystis is a disease reported frequently that can be fatal when it occurs at early stages. Skin flukes such as Benedenia seriolae and Neobenedenia girellae as well as other parasites bacteria and viruses mentioned herein, which have caused substantial losses in Asian enterprises, but have not been identified yet in greater amberjack farmed in the Mediterranean, should be considered as potential threats. |
Tsertou, M I; Chatzifotis, S; Fontanillas, R; Cotou, E; Fountoulaki, E; Antonopoulou, E; Katharios, P Aquaculture, 521 , pp. 735052, 2020, ISSN: 00448486. @article{tsertou_effect_2020, title = {The effect of dietary vitamin D3, minerals (Ca, P) and plant-protein sources in the development of systemic granulomatosis in meagre (Argyrosomus regius, Asso, 1801)}, author = {M I Tsertou and S Chatzifotis and R Fontanillas and E Cotou and E Fountoulaki and E Antonopoulou and P Katharios}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848619326626}, doi = {10.1016/j.aquaculture.2020.735052}, issn = {00448486}, year = {2020}, date = {2020-05-01}, urldate = {2020-08-31}, journal = {Aquaculture}, volume = {521}, pages = {735052}, abstract = {Systemic Granulomatosis (SG) affects the majority of cultured meagre (Argyrosomus regius). This pathological condition, whose aetiology is still not known, is identified by multiple granulomas in a variety of organs that become calcified and necrotic with time. The available evidence supports the hypothesis that SG is a metabolic disorder associated with nutritional imbalances, and the present study examined the role of dietary vitamin D3, minerals (Ca, P) and plant-protein sources in the development of the disease. The use of plant-protein in the diet formulation exacerbated the progression of SG, while vitamin D3 did not prevent its occurrence. On the other hand, the severity of SG was improved by increasing the P content in the feed. However, the exact irritant that causes this inflammatory response in meagre is still unknown and more research needs to examine other nutritional factors.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Systemic Granulomatosis (SG) affects the majority of cultured meagre (Argyrosomus regius). This pathological condition, whose aetiology is still not known, is identified by multiple granulomas in a variety of organs that become calcified and necrotic with time. The available evidence supports the hypothesis that SG is a metabolic disorder associated with nutritional imbalances, and the present study examined the role of dietary vitamin D3, minerals (Ca, P) and plant-protein sources in the development of the disease. The use of plant-protein in the diet formulation exacerbated the progression of SG, while vitamin D3 did not prevent its occurrence. On the other hand, the severity of SG was improved by increasing the P content in the feed. However, the exact irritant that causes this inflammatory response in meagre is still unknown and more research needs to examine other nutritional factors. |
Kokou, Fotini; Bastias, Roberto; Kokkari, Konstantina; Katharios, Pantelis; Cotou, Efthimia; Seimenis, Nikos; Vasilakis, Manolis; Papandroulakis, Nikos; Henry, Morgane; Rigos, George Surplus of dietary micronutrients promotes antioxidant defense and improves fin erosions in European seabass (Dicentrarchus labrax) fry Journal Article Aquaculture, 523 , pp. 735224, 2020, ISSN: 0044-8486. @article{kokou_surplus_2020, title = {Surplus of dietary micronutrients promotes antioxidant defense and improves fin erosions in European seabass (Dicentrarchus labrax) fry}, author = {Fotini Kokou and Roberto Bastias and Konstantina Kokkari and Pantelis Katharios and Efthimia Cotou and Nikos Seimenis and Manolis Vasilakis and Nikos Papandroulakis and Morgane Henry and George Rigos}, url = {http://www.sciencedirect.com/science/article/pii/S0044848619330868}, doi = {https://doi.org/10.1016/j.aquaculture.2020.735224}, issn = {0044-8486}, year = {2020}, date = {2020-01-01}, journal = {Aquaculture}, volume = {523}, pages = {735224}, abstract = {Dietary micronutrient supplementation can serve as skin health promotor to prevent from natural infections and can be applied to decrease the use of antibacterial agents and their impact on the environment. Such supplementation has indicated anti-inflammatory and antioxidant properties in farmed fish. In this study, dietary supplementation of zinc, selenium, ascorbic acid and niacin, at levels 2 to 3-fold higher than recommended, was evaluated as a measure to promote skin health and antioxidant defense in European seabass, Dicentrarchus labrax, fry. For this purpose, fish survival, growth performance, fin and skin erosions as well as whole-fish antioxidant enzyme activity (catalase, selenium (Se)-independent and dependent glutathione peroxidase) were assessed. Moreover, bacterial load in the rearing water and skin mucus were evaluated to assess the potential of the dietary micronutrient mixture as a preventive from natural infections. A group fed with a diet including these micronutrients at recommended levels served as a control. Surplus of these micronutrients significantly reduced the percentage of fish with eroded fins, while also induced the activity of catalase and Se-dependent glutathione peroxidase. Fish growth and bacterial loads in the water and fish skin mucus were not affected, whereas no skin lesions were observed. Overall, a mineral- and vitamin-supplemented diet at levels higher than recommended seems to act as a promotor of skin health and prevent from fin erosions, potentially through an increase in the antioxidant defense system.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dietary micronutrient supplementation can serve as skin health promotor to prevent from natural infections and can be applied to decrease the use of antibacterial agents and their impact on the environment. Such supplementation has indicated anti-inflammatory and antioxidant properties in farmed fish. In this study, dietary supplementation of zinc, selenium, ascorbic acid and niacin, at levels 2 to 3-fold higher than recommended, was evaluated as a measure to promote skin health and antioxidant defense in European seabass, Dicentrarchus labrax, fry. For this purpose, fish survival, growth performance, fin and skin erosions as well as whole-fish antioxidant enzyme activity (catalase, selenium (Se)-independent and dependent glutathione peroxidase) were assessed. Moreover, bacterial load in the rearing water and skin mucus were evaluated to assess the potential of the dietary micronutrient mixture as a preventive from natural infections. A group fed with a diet including these micronutrients at recommended levels served as a control. Surplus of these micronutrients significantly reduced the percentage of fish with eroded fins, while also induced the activity of catalase and Se-dependent glutathione peroxidase. Fish growth and bacterial loads in the water and fish skin mucus were not affected, whereas no skin lesions were observed. Overall, a mineral- and vitamin-supplemented diet at levels higher than recommended seems to act as a promotor of skin health and prevent from fin erosions, potentially through an increase in the antioxidant defense system. |
Mladineo, Ivona; Hrabar, Jerko; Vidjak, Olja; Bočina, Ivana; Čolak, Slavica; Katharios, Pantelis; Cascarano, Maria Chiara; Keklikoglou, Kleoniki; Volpatti, Donatella; Beraldo, Paola Host-parasite interaction between parasitic cy-mothoid ceratothoa oestroides and its host, farmed european sea bass (Dicentrarchus labrax) Journal Article Pathogens, 9 (3), pp. 1–19, 2020, ISSN: 20760817. @article{mladineo_host-parasite_2020, title = {Host-parasite interaction between parasitic cy-mothoid ceratothoa oestroides and its host, farmed european sea bass (Dicentrarchus labrax)}, author = {Ivona Mladineo and Jerko Hrabar and Olja Vidjak and Ivana Bočina and Slavica Čolak and Pantelis Katharios and Maria Chiara Cascarano and Kleoniki Keklikoglou and Donatella Volpatti and Paola Beraldo}, doi = {10.3390/pathogens9030230}, issn = {20760817}, year = {2020}, date = {2020-01-01}, journal = {Pathogens}, volume = {9}, number = {3}, pages = {1--19}, abstract = {Parasitic isopod Ceratothoa oestroides (Cymothoidea, Isopoda) is a common and generalist buccal cavity-dweller in marine fish, recognised for its detrimental effect in fingerling and juvenile farmed European sea bass (Dicentrarchus labrax). Although distributed throughout the Mediterra-nean, the isopod provokes acute outbreaks mainly limited to particular endemic areas in Croatia (Adriatic Sea) and Greece (Aegean Sea). While numerous studies have previously evidenced its gross effect on farmed fish (i.e. decreased condition index, slower growth rate, lethargy and mortal-ity), details on the host-parasite interaction are still lacking. Therefore, using a multimethodological approach, we closely examined the structure and appearance of isopod body parts acting in the attachment and feeding (stereomicroscopy, scanning and transmission electron microscopy), and the extent of host tissues damage (histology, immunohistochemistry, micro-computational tomog-raphy) induced by parasitation. Interestingly, while hematophagous nature of the parasite has been previously postulated we found no unambiguous data to support this; we observed host tissues fragmentation and extensive hyperplasia at the parasitation site, and no structures indicative of heme detoxifying mechanisms in the parasite gut, or other traces of a blood meal. The bacterial biofilm covering C. oestroides mouthparts and pereopods suggests that the isopod may play a role in conveying secondary pathogens to the infected host, or alternatively, it serves the parasite in normal interaction with its environment.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Parasitic isopod Ceratothoa oestroides (Cymothoidea, Isopoda) is a common and generalist buccal cavity-dweller in marine fish, recognised for its detrimental effect in fingerling and juvenile farmed European sea bass (Dicentrarchus labrax). Although distributed throughout the Mediterra-nean, the isopod provokes acute outbreaks mainly limited to particular endemic areas in Croatia (Adriatic Sea) and Greece (Aegean Sea). While numerous studies have previously evidenced its gross effect on farmed fish (i.e. decreased condition index, slower growth rate, lethargy and mortal-ity), details on the host-parasite interaction are still lacking. Therefore, using a multimethodological approach, we closely examined the structure and appearance of isopod body parts acting in the attachment and feeding (stereomicroscopy, scanning and transmission electron microscopy), and the extent of host tissues damage (histology, immunohistochemistry, micro-computational tomog-raphy) induced by parasitation. Interestingly, while hematophagous nature of the parasite has been previously postulated we found no unambiguous data to support this; we observed host tissues fragmentation and extensive hyperplasia at the parasitation site, and no structures indicative of heme detoxifying mechanisms in the parasite gut, or other traces of a blood meal. The bacterial biofilm covering C. oestroides mouthparts and pereopods suggests that the isopod may play a role in conveying secondary pathogens to the infected host, or alternatively, it serves the parasite in normal interaction with its environment. |
Katharios, Pantelis; Varvarigos, Panos; Keklikoglou, Kleoniki; Ruetten, Maja; Sojan, Jerry; Akter, Morgina; Cascarano, Maria Chiara; Tsertou, Maria Ioanna; Kokkari, Constantina Parasites & Vectors, 13 (1), pp. 27, 2020, ISSN: 17563305, (ISBN: 1307102038887 Publisher: BioMed Central). @article{katharios_native_2020, title = {Native parasite affecting an introduced host in aquaculture: cardiac henneguyosis in the red seabream Pagrus major Temminck & Schlegel (Perciformes: Sparidae) caused by Henneguya aegea n. sp. (Myxosporea: Myxobolidae)}, author = {Pantelis Katharios and Panos Varvarigos and Kleoniki Keklikoglou and Maja Ruetten and Jerry Sojan and Morgina Akter and Maria Chiara Cascarano and Maria Ioanna Tsertou and Constantina Kokkari}, url = {https://doi.org/10.1186/s13071-020-3888-7}, doi = {10.1186/s13071-020-3888-7}, issn = {17563305}, year = {2020}, date = {2020-01-01}, journal = {Parasites & Vectors}, volume = {13}, number = {1}, pages = {27}, abstract = {BACKGROUND: Henneguya Thélohan, 1892 (Myxobolidae) is one of the most species-rich genera of myxosporean parasites infecting fish. Although common in nature, there are few reports of these parasites causing important disease in aquaculture. In this paper, we describe a new species of Henneguya infecting Pagrus major (Temminck & Schlegel), a fish host introduced to the Mediterranean Sea from Japan in the late 1980s. RESULTS: Large plasmodia of the parasite were found in the bulbus arteriosus and in the ventricle of the infected fish. Spores were found mainly in the kidney and heart and were accompanied by melanized macrophages or vascular intimal proliferation mixed with a mild non-suppurative response, respectively. Comparisons of morphometric data for spore and polar capsule length and width, suggest a unique combination of features in the newly described species. Molecular analysis, based on 18S rDNA sequence of the parasite, followed by phylogenetic analysis, indicated that the parasite described here is a novel species of Henneguya, clustered with the marine congeneric species. CONCLUSIONS: Henneguya aegea n. sp. infects in aquaculture P. major, a host introduced as eggs to the Mediterranean from Japan. Despite the high host specificity of the myxobolid parasites, H. aegea n. sp. seems to be able to use P. major as a host and propagate successfully, causing morbidity and mortality. This could result in spillback of the new species from high density cultured non-native P. major to native fish hosts.}, note = {ISBN: 1307102038887 Publisher: BioMed Central}, keywords = {}, pubstate = {published}, tppubtype = {article} } BACKGROUND: Henneguya Thélohan, 1892 (Myxobolidae) is one of the most species-rich genera of myxosporean parasites infecting fish. Although common in nature, there are few reports of these parasites causing important disease in aquaculture. In this paper, we describe a new species of Henneguya infecting Pagrus major (Temminck & Schlegel), a fish host introduced to the Mediterranean Sea from Japan in the late 1980s. RESULTS: Large plasmodia of the parasite were found in the bulbus arteriosus and in the ventricle of the infected fish. Spores were found mainly in the kidney and heart and were accompanied by melanized macrophages or vascular intimal proliferation mixed with a mild non-suppurative response, respectively. Comparisons of morphometric data for spore and polar capsule length and width, suggest a unique combination of features in the newly described species. Molecular analysis, based on 18S rDNA sequence of the parasite, followed by phylogenetic analysis, indicated that the parasite described here is a novel species of Henneguya, clustered with the marine congeneric species. CONCLUSIONS: Henneguya aegea n. sp. infects in aquaculture P. major, a host introduced as eggs to the Mediterranean from Japan. Despite the high host specificity of the myxobolid parasites, H. aegea n. sp. seems to be able to use P. major as a host and propagate successfully, causing morbidity and mortality. This could result in spillback of the new species from high density cultured non-native P. major to native fish hosts. |
Smyrli, Maria; Katharios, Pantelis Aeromonas spp. Incollection Zrnčić, Snježana (Ed.): Diagnostic manual for the main pathogens in European seabass and Gilthead seabream aquaculture, (75), pp. 107–116, CIHEAM, Zaragoza, 2020, ISBN: 978-2-85352-596-1. @incollection{smyrli_aeromonas_2020, title = {Aeromonas spp.}, author = {Maria Smyrli and Pantelis Katharios}, editor = {Snježana Zrnčić}, url = {http://om.ciheam.org/om/pdf/b75/b75.pdf}, isbn = {978-2-85352-596-1}, year = {2020}, date = {2020-01-01}, booktitle = {Diagnostic manual for the main pathogens in European seabass and Gilthead seabream aquaculture}, number = {75}, pages = {107--116}, publisher = {CIHEAM}, address = {Zaragoza}, series = {Options Méditerranéennes, Series B: Studies and Research}, keywords = {}, pubstate = {published}, tppubtype = {incollection} } |
Misol, Gerald N; Kokkari, Constantina; Katharios, Pantelis Biological and Genomic Characterization of a Novel Jumbo Bacteriophage, vB_VhaM_pir03 with Broad Host Lytic Activity against Vibrio harveyi Journal Article Pathogens, 9 (12), pp. 1051, 2020, ISSN: 2076-0817. @article{misol_biological_2020, title = {Biological and Genomic Characterization of a Novel Jumbo Bacteriophage, vB_VhaM_pir03 with Broad Host Lytic Activity against Vibrio harveyi}, author = {Gerald N Misol and Constantina Kokkari and Pantelis Katharios}, url = {https://www.mdpi.com/2076-0817/9/12/1051 https://imbbc.hcmr.gr/wp-content/uploads/2020/12/2020-Misol-Pathogens-53.pdf}, doi = {10.3390/pathogens9121051}, issn = {2076-0817}, year = {2020}, date = {2020-01-01}, urldate = {2020-12-29}, journal = {Pathogens}, volume = {9}, number = {12}, pages = {1051}, abstract = {Vibrio harveyi is a Gram-negative marine bacterium that causes major disease outbreaks and economic losses in aquaculture. Phage therapy has been considered as a potential alternative to antibiotics however, candidate bacteriophages require comprehensive characterization for a safe and practical phage therapy. In this work, a lytic novel jumbo bacteriophage, vB_VhaM_pir03 belonging to the Myoviridae family was isolated and characterized against V. harveyi type strain DSM19623. It had broad host lytic activity against 31 antibiotic-resistant strains of V. harveyi, V. alginolyticus, V. campbellii and V. owensii. Adsorption time of vB_VhaM_pir03 was determined at 6 min while the latent-phase was at 40 min and burst-size at 75 pfu/mL. vB_VhaM_pir03 was able to lyse several host strains at multiplicity-of-infections (MOI) 0.1 to 10. The genome of vB_VhaM_pir03 consists of 286,284 base pairs with 334 predicted open reading frames (ORFs). No virulence, antibiotic resistance, integrase encoding genes and transducing potential were detected. Phylogenetic and phylogenomic analysis showed that vB_VhaM_pir03 is a novel bacteriophage displaying the highest similarity to another jumbo phage, vB_BONAISHI infecting Vibrio coralliilyticus. Experimental phage therapy trial using brine shrimp, Artemia salina infected with V. harveyi demonstrated that vB_VhaM_pir03 was able to significantly reduce mortality 24 h post infection when administered at MOI 0.1 which suggests that it can be an excellent candidate for phage therapy.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Vibrio harveyi is a Gram-negative marine bacterium that causes major disease outbreaks and economic losses in aquaculture. Phage therapy has been considered as a potential alternative to antibiotics however, candidate bacteriophages require comprehensive characterization for a safe and practical phage therapy. In this work, a lytic novel jumbo bacteriophage, vB_VhaM_pir03 belonging to the Myoviridae family was isolated and characterized against V. harveyi type strain DSM19623. It had broad host lytic activity against 31 antibiotic-resistant strains of V. harveyi, V. alginolyticus, V. campbellii and V. owensii. Adsorption time of vB_VhaM_pir03 was determined at 6 min while the latent-phase was at 40 min and burst-size at 75 pfu/mL. vB_VhaM_pir03 was able to lyse several host strains at multiplicity-of-infections (MOI) 0.1 to 10. The genome of vB_VhaM_pir03 consists of 286,284 base pairs with 334 predicted open reading frames (ORFs). No virulence, antibiotic resistance, integrase encoding genes and transducing potential were detected. Phylogenetic and phylogenomic analysis showed that vB_VhaM_pir03 is a novel bacteriophage displaying the highest similarity to another jumbo phage, vB_BONAISHI infecting Vibrio coralliilyticus. Experimental phage therapy trial using brine shrimp, Artemia salina infected with V. harveyi demonstrated that vB_VhaM_pir03 was able to significantly reduce mortality 24 h post infection when administered at MOI 0.1 which suggests that it can be an excellent candidate for phage therapy. |
2019 |
Anastasiou, Thekla I; Mandalakis, Manolis; Krigas, Nikos; Vézignol, Thomas; Lazari, Diamanto; Katharios, Pantelis; Dailianis, Thanos; Antonopoulou, Efthimia Molecules, 25 (1), pp. 148, 2019, ISSN: 1420-3049. @article{anastasiou_comparative_2019, title = {Comparative Evaluation of Essential Oils from Medicinal-Aromatic Plants of Greece: Chemical Composition, Antioxidant Capacity and Antimicrobial Activity against Bacterial Fish Pathogens}, author = {Thekla I Anastasiou and Manolis Mandalakis and Nikos Krigas and Thomas Vézignol and Diamanto Lazari and Pantelis Katharios and Thanos Dailianis and Efthimia Antonopoulou}, url = {https://www.mdpi.com/1420-3049/25/1/148}, doi = {10.3390/molecules25010148}, issn = {1420-3049}, year = {2019}, date = {2019-12-01}, urldate = {2020-08-09}, journal = {Molecules}, volume = {25}, number = {1}, pages = {148}, abstract = {The administration of antibiotics in aquaculture has raised concern about the impact of their overuse in marine ecosystems, seafood safety and consumers’ health. This “green consumerism” has forced researchers to find new alternatives against fish pathogens. The present study focused on 12 Mediterranean medicinal-aromatic plants as potential antimicrobials and antioxidant agents that could be used in fish aquaculture. In vitro assays showed that the essential oils (EOs) from all studied plants had anti-bacterial and antioxidant properties, with their efficacy being dependent on their chemical composition. More specifically, EOs rich in carvacrol, p-cymene and γ-terpinene exhibited not only the strongest inhibitory activity against the growth of bacterial pathogens (inhibitory concentration: 26–88 μg mL−1), but also the greatest total antioxidant capacity (ABTS: 2591–5879 μmole mL−1; CUPRAC: 931–2733 μmole mL−1). These compounds were mainly found in the EOs from Greek oregano (Origanum vulgare subsp. hirtum), Spanish oregano (Thymbra capitata) and savoury (Satureja thymbra) collected from cultivations in Greece. The specific EOs stand out as promising candidates for the treatment of bacterial diseases and oxidative stress in farmed fish. Further in vivo experiments are needed to fully understand the effects of EO dietary supplementation on fish farming processes.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The administration of antibiotics in aquaculture has raised concern about the impact of their overuse in marine ecosystems, seafood safety and consumers’ health. This “green consumerism” has forced researchers to find new alternatives against fish pathogens. The present study focused on 12 Mediterranean medicinal-aromatic plants as potential antimicrobials and antioxidant agents that could be used in fish aquaculture. In vitro assays showed that the essential oils (EOs) from all studied plants had anti-bacterial and antioxidant properties, with their efficacy being dependent on their chemical composition. More specifically, EOs rich in carvacrol, p-cymene and γ-terpinene exhibited not only the strongest inhibitory activity against the growth of bacterial pathogens (inhibitory concentration: 26–88 μg mL−1), but also the greatest total antioxidant capacity (ABTS: 2591–5879 μmole mL−1; CUPRAC: 931–2733 μmole mL−1). These compounds were mainly found in the EOs from Greek oregano (Origanum vulgare subsp. hirtum), Spanish oregano (Thymbra capitata) and savoury (Satureja thymbra) collected from cultivations in Greece. The specific EOs stand out as promising candidates for the treatment of bacterial diseases and oxidative stress in farmed fish. Further in vivo experiments are needed to fully understand the effects of EO dietary supplementation on fish farming processes. |
Mladineo, ; Hrabar, ; Smodlaka, ; Palmer, ; Sakamaki, ; Keklikoglou, ; Katharios, Functional Ultrastructure of the Excretory Gland Cell in Zoonotic Anisakids (Anisakidae, Nematoda) Journal Article Cells, 8 (11), pp. 1451, 2019, ISSN: 2073-4409. @article{mladineo_functional_2019, title = {Functional Ultrastructure of the Excretory Gland Cell in Zoonotic Anisakids (Anisakidae, Nematoda)}, author = {Mladineo and Hrabar and Smodlaka and Palmer and Sakamaki and Keklikoglou and Katharios}, url = {https://www.mdpi.com/2073-4409/8/11/1451}, doi = {10.3390/cells8111451}, issn = {2073-4409}, year = {2019}, date = {2019-11-01}, urldate = {2020-08-21}, journal = {Cells}, volume = {8}, number = {11}, pages = {1451}, abstract = {Excretory and secretory products are crucial for parasite infectivity and host immunomodulation, but the functioning and ultrastructure of the excretory gland cell (EC) that produces these products are still scarcely understood and described. In light of growing reports on anisakiasis cases in Europe, we aimed to characterise the EC of larval Anisakis pegreffii and adult Pseudoterranova azarasi. In the latter, EC starts 0.85 mm from the head tip, measuring 1.936 × 0.564 mm. Larval EC shows a long nucleus with thorn-like extravaginations toward the cytoplasm, numerous electron-dense and -lucent secretory granules spanning from the perinuclear to subplasmalemmal space, an elevated number of free ribosomes, small, spherical mitochondria with few cristae and a laminated matrix, small and few Golgi apparatuses, and few endoplasmic reticula, with wide cisternae complexes. Ultrastructure suggests that anaerobic glycolysis is the main metabolic pathway, obtained through nutrient endocytosis across the pseudocoelomic surface of the EC plasmalemma and its endocytic canaliculi. Thorn-like extravaginations of EC karyotheca likely mediate specific processes (Ca2+ signaling, gene expression, transport, nuclear lipid metabolism) into the extremely wide EC cytosol, enabling focal delivery of a signal to specific sites in a short time. These functional annotations of parasitic EC should help to clarify anisakiasis pathogenesis.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Excretory and secretory products are crucial for parasite infectivity and host immunomodulation, but the functioning and ultrastructure of the excretory gland cell (EC) that produces these products are still scarcely understood and described. In light of growing reports on anisakiasis cases in Europe, we aimed to characterise the EC of larval Anisakis pegreffii and adult Pseudoterranova azarasi. In the latter, EC starts 0.85 mm from the head tip, measuring 1.936 × 0.564 mm. Larval EC shows a long nucleus with thorn-like extravaginations toward the cytoplasm, numerous electron-dense and -lucent secretory granules spanning from the perinuclear to subplasmalemmal space, an elevated number of free ribosomes, small, spherical mitochondria with few cristae and a laminated matrix, small and few Golgi apparatuses, and few endoplasmic reticula, with wide cisternae complexes. Ultrastructure suggests that anaerobic glycolysis is the main metabolic pathway, obtained through nutrient endocytosis across the pseudocoelomic surface of the EC plasmalemma and its endocytic canaliculi. Thorn-like extravaginations of EC karyotheca likely mediate specific processes (Ca2+ signaling, gene expression, transport, nuclear lipid metabolism) into the extremely wide EC cytosol, enabling focal delivery of a signal to specific sites in a short time. These functional annotations of parasitic EC should help to clarify anisakiasis pathogenesis. |
Smyrli, Maria; Triga, Adriana; Dourala, Nancy; Varvarigos, Panos; Pavlidis, Michael; Quoc, Viet Ha; Katharios, Pantelis Comparative study on a novel pathogen of european seabass. Diversity of Aeromonas veronii in the aegean sea Journal Article Microorganisms, 7 (11), 2019, ISSN: 20762607. @article{smyrli_comparative_2019, title = {Comparative study on a novel pathogen of european seabass. Diversity of Aeromonas veronii in the aegean sea}, author = {Maria Smyrli and Adriana Triga and Nancy Dourala and Panos Varvarigos and Michael Pavlidis and Viet Ha Quoc and Pantelis Katharios}, doi = {10.3390/microorganisms7110504}, issn = {20762607}, year = {2019}, date = {2019-01-01}, journal = {Microorganisms}, volume = {7}, number = {11}, abstract = {Aeromonas veronii is an emerging pathogen causing severe pathology and mortalities in European seabass aquaculture in the Aegean Sea, Mediterranean. More than 50 strains of the pathogen were characterized biochemically and genetically in order to study the epidemiology of the disease, as well as the phylogeny and virulence of the bacterium. Based on the phenotypic characteristics, the isolates form three groups consisting of: (a) the West Aegean Sea, non-motile, non-pigment-producing strains, (b) the West Aegean Sea, motile, and pigment-producing strains and (c) the East Aegean Sea motile strains that produce minute amounts of pigment. All strains were highly similar at the genomic level; however, the pattern of West/East geographic origin was reflected in biochemical properties, in general genomic level comparison and in the putative virulent factors studied. Type VI secretion system was not detected in the western strains. The outer membrane protein (OMP) profile which contains proteins that are putative antigenic factors, was very similar between strains from the different areas. Although most of the OMPs were detected in all strains with great sequence similarity, diversification according to geographic origin was evident in known antigenic factors such as the maltoporin LamB. A systematic comparative analysis of the strains is presented and discussed in view of the emergence of A. veronii as a significant pathogen for the Mediterranean aquaculture.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Aeromonas veronii is an emerging pathogen causing severe pathology and mortalities in European seabass aquaculture in the Aegean Sea, Mediterranean. More than 50 strains of the pathogen were characterized biochemically and genetically in order to study the epidemiology of the disease, as well as the phylogeny and virulence of the bacterium. Based on the phenotypic characteristics, the isolates form three groups consisting of: (a) the West Aegean Sea, non-motile, non-pigment-producing strains, (b) the West Aegean Sea, motile, and pigment-producing strains and (c) the East Aegean Sea motile strains that produce minute amounts of pigment. All strains were highly similar at the genomic level; however, the pattern of West/East geographic origin was reflected in biochemical properties, in general genomic level comparison and in the putative virulent factors studied. Type VI secretion system was not detected in the western strains. The outer membrane protein (OMP) profile which contains proteins that are putative antigenic factors, was very similar between strains from the different areas. Although most of the OMPs were detected in all strains with great sequence similarity, diversification according to geographic origin was evident in known antigenic factors such as the maltoporin LamB. A systematic comparative analysis of the strains is presented and discussed in view of the emergence of A. veronii as a significant pathogen for the Mediterranean aquaculture. |
León, Marcela; Kokkari, Constantina; García, Katherine; Castillo, Daniel; Katharios, Pantelis; Bastías, Roberto Diversification of vibrio anguillarum driven by the bacteriophage CHOED Journal Article Frontiers in Microbiology, 10 , pp. 1396, 2019, ISSN: 1664302X. @article{leon_diversification_2019, title = {Diversification of vibrio anguillarum driven by the bacteriophage CHOED}, author = {Marcela León and Constantina Kokkari and Katherine García and Daniel Castillo and Pantelis Katharios and Roberto Bastías}, doi = {10.3389/fmicb.2019.01396}, issn = {1664302X}, year = {2019}, date = {2019-01-01}, journal = {Frontiers in Microbiology}, volume = {10}, pages = {1396}, abstract = {Bacteriophages are an important factor in bacterial evolution. Some reports suggest that lytic bacteriophages can select for resistant mutant strains with reduced virulence. The present study explores the role of the CHOED bacteriophage in the diversification and virulence of its host Vibrio anguillarum. Nine phage-resistant strains were analyzed for their phenotype and different virulence factors, showing alterations in their fitness, motility, biofilm formation, lipopolysaccharide profiles and/or protease activity. Seven of the nine phage-resistant strains showed virulence reduction in a Sparus aurata larvae model. However, this is not generalized since two of the resistant strains show equal virulence compared with the parental strain. The genomic analysis of representative resistant strains displayed that the majority of the mutations are specific for each isolate, affecting genes related to lipopolysaccharide biosynthesis, quorum sensing, motility, toxin and membrane transport. The observed mutations were coherent with the phenotypic and virulence differences observed. These results suggest that the CHOED phage acts as a selective pressure on V. anguillarum, allowing proliferation of resistant strains with different genotypes, phenotypes and degrees of virulence, contributing to bacterial diversification.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Bacteriophages are an important factor in bacterial evolution. Some reports suggest that lytic bacteriophages can select for resistant mutant strains with reduced virulence. The present study explores the role of the CHOED bacteriophage in the diversification and virulence of its host Vibrio anguillarum. Nine phage-resistant strains were analyzed for their phenotype and different virulence factors, showing alterations in their fitness, motility, biofilm formation, lipopolysaccharide profiles and/or protease activity. Seven of the nine phage-resistant strains showed virulence reduction in a Sparus aurata larvae model. However, this is not generalized since two of the resistant strains show equal virulence compared with the parental strain. The genomic analysis of representative resistant strains displayed that the majority of the mutations are specific for each isolate, affecting genes related to lipopolysaccharide biosynthesis, quorum sensing, motility, toxin and membrane transport. The observed mutations were coherent with the phenotypic and virulence differences observed. These results suggest that the CHOED phage acts as a selective pressure on V. anguillarum, allowing proliferation of resistant strains with different genotypes, phenotypes and degrees of virulence, contributing to bacterial diversification. |
Kalatzis, Panos G; Carstens, Alexander B; Katharios, Pantelis; Castillo, Daniel; Hansen, Lars H; Middelboe, Mathias Complete Genome Sequence of Vibrio anguillarum Nontailed Bacteriophage NO16 Journal Article Microbiology Resource Announcements, 8 (15), pp. 11–12, 2019, ISSN: 2576-098X, (ISBN: 9781603271646). @article{kalatzis_complete_2019, title = {Complete Genome Sequence of Vibrio anguillarum Nontailed Bacteriophage NO16}, author = {Panos G Kalatzis and Alexander B Carstens and Pantelis Katharios and Daniel Castillo and Lars H Hansen and Mathias Middelboe}, doi = {10.1128/mra.00020-19}, issn = {2576-098X}, year = {2019}, date = {2019-01-01}, journal = {Microbiology Resource Announcements}, volume = {8}, number = {15}, pages = {11--12}, abstract = {A rare nontailed virus designated NO16 was isolated against Vibrio anguillarum , a major aquaculture pathogen for both fish and shellfish. Here, we announce the 10,594-bp genome sequence of Vibrio phage NO16 with a 23-gene content. A rare nontailed virus designated NO16 was isolated against Vibrio anguillarum , a major aquaculture pathogen for both fish and shellfish. Here, we announce the 10,594-bp genome sequence of Vibrio phage NO16 with a 23-gene content.}, note = {ISBN: 9781603271646}, keywords = {}, pubstate = {published}, tppubtype = {article} } A rare nontailed virus designated NO16 was isolated against Vibrio anguillarum , a major aquaculture pathogen for both fish and shellfish. Here, we announce the 10,594-bp genome sequence of Vibrio phage NO16 with a 23-gene content. A rare nontailed virus designated NO16 was isolated against Vibrio anguillarum , a major aquaculture pathogen for both fish and shellfish. Here, we announce the 10,594-bp genome sequence of Vibrio phage NO16 with a 23-gene content. |
Katharios, Pantelis; Kalatzis, Panos G; Kokkari, Constantina; Pavlidis, Michail; Wang, Qiyao Frontiers in Microbiology, 10 , pp. 141, 2019, ISSN: 1664302X. @article{katharios_characterization_2019, title = {Characterization of a highly virulent Edwardsiella anguillarum strain isolated from Greek aquaculture, and a spontaneously induced prophage therein}, author = {Pantelis Katharios and Panos G Kalatzis and Constantina Kokkari and Michail Pavlidis and Qiyao Wang}, doi = {10.3389/fmicb.2019.00141}, issn = {1664302X}, year = {2019}, date = {2019-01-01}, journal = {Frontiers in Microbiology}, volume = {10}, pages = {141}, abstract = {Edwardsiella-associated outbreaks are increasingly reported on both marine and freshwater aquaculture setups, accounting for severe financial and biomass losses. E. tarda, E. ictaluri, and E. hoshinae have been the traditional causative agents of edwardsiellosis in aquaculture, however, intensive studies due to the significance of the disease have just recently revealed two more species, E. piscicida and E. anguillarum. Whole genome sequencing that was conducted on the strain EA011113, isolated from farmed Diplodus puntazzo after an edwardsiellosis outbreak in Greece, confirmed it as a new clinical strain of E. anguillarum. Extensive phylogenetic analysis showed that this Greek strain is closely related to an Israeli E. piscicida-like clinical strain, isolated from diseased groupers, Epinephelus aeneus and E. marginatus in Red Sea. Bioinformatic analyses of E. anguillarum strain EA011113 unveiled a wide repertoire of potential virulence factors, the effect of which was corroborated by the mortalities that the strain induced in adult zebrafish, Danio rerio, under different levels of infection intensity (LD50 after 48 h: 1.85 × 104 cfu/fish). This strain was non-motile and according to electron microscopy lacked flagella, a fact that is not typical for E. anguillarum. Comparative genomic analysis revealed a deletion of 36 nt found in the flagellar biosynthetic gene (FlhB) that could explain that trait. Further in silico analysis revealed an intact prophage that was integrated in the bacterial genome. Following spontaneous induction, the phage was isolated, purified, characterized and independently sequenced, confirming its viability as a free, inducible virion as well. Separate genomic analysis of the prophage implies a plausible case of lysogenic conversion. Focusing on edwardsiellosis as a rapidly emerging aquaculture disease on a global scale, this work offers some insight into the virulence, fitness, and potential lysogenic conversion of a of a newly described, yet highly pathogenic, strain of E. anguillarum.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Edwardsiella-associated outbreaks are increasingly reported on both marine and freshwater aquaculture setups, accounting for severe financial and biomass losses. E. tarda, E. ictaluri, and E. hoshinae have been the traditional causative agents of edwardsiellosis in aquaculture, however, intensive studies due to the significance of the disease have just recently revealed two more species, E. piscicida and E. anguillarum. Whole genome sequencing that was conducted on the strain EA011113, isolated from farmed Diplodus puntazzo after an edwardsiellosis outbreak in Greece, confirmed it as a new clinical strain of E. anguillarum. Extensive phylogenetic analysis showed that this Greek strain is closely related to an Israeli E. piscicida-like clinical strain, isolated from diseased groupers, Epinephelus aeneus and E. marginatus in Red Sea. Bioinformatic analyses of E. anguillarum strain EA011113 unveiled a wide repertoire of potential virulence factors, the effect of which was corroborated by the mortalities that the strain induced in adult zebrafish, Danio rerio, under different levels of infection intensity (LD50 after 48 h: 1.85 × 104 cfu/fish). This strain was non-motile and according to electron microscopy lacked flagella, a fact that is not typical for E. anguillarum. Comparative genomic analysis revealed a deletion of 36 nt found in the flagellar biosynthetic gene (FlhB) that could explain that trait. Further in silico analysis revealed an intact prophage that was integrated in the bacterial genome. Following spontaneous induction, the phage was isolated, purified, characterized and independently sequenced, confirming its viability as a free, inducible virion as well. Separate genomic analysis of the prophage implies a plausible case of lysogenic conversion. Focusing on edwardsiellosis as a rapidly emerging aquaculture disease on a global scale, this work offers some insight into the virulence, fitness, and potential lysogenic conversion of a of a newly described, yet highly pathogenic, strain of E. anguillarum. |
2018 |
Kokkari, C; Sarropoulou, E; Bastias, R; Mandalakis, M; Katharios, P Isolation and characterization of a novel bacteriophage infecting Vibrio alginolyticus Journal Article Archives of Microbiology, 200 (5), pp. 707–718, 2018, ISSN: 03028933, (Publisher: Springer Verlag). @article{kokkari_isolation_2018, title = {Isolation and characterization of a novel bacteriophage infecting Vibrio alginolyticus}, author = {C Kokkari and E Sarropoulou and R Bastias and M Mandalakis and P Katharios}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040930909&doi=10.1007%2fs00203-018-1480-8&partnerID=40&md5=b22268a55a9ed98e93e66d4448fbe221}, doi = {10.1007/s00203-018-1480-8}, issn = {03028933}, year = {2018}, date = {2018-01-01}, journal = {Archives of Microbiology}, volume = {200}, number = {5}, pages = {707--718}, abstract = {Vibrio alginolyticus is a common marine bacterium implicated in disease outbreaks in marine farmed fish and invertebrates. Due to the inappropriate use of antibiotics in aquaculture, alternative therapies have been proposed. One of the most promising options is the use of lytic bacteriophages to control pathogenic bacteria. This work describes the isolation and characterization of a lytic phage (VEN) against a V. alginolyticus strain (V2) isolated from a disease outbreak in common dentex (Dentex dentex) cultured at the Hellenic Centre for Marine Research (HCMR) in Crete, Greece. The bacteriophage is morphologically similar to phages from Podoviridae family and remained stable for 1 year at 4 °C and over 1 h when kept at 50 °C. VEN was able to lyse the host bacteria at several multiplicity of infection (MOI) (0.1–100) in liquid cultures. However, it was unable to infect other V. alginolyticus strains. Its genome consists of 44,603 bp with a GC content of 43.5%, while sequence analysis revealed the presence of 54 potential ORFs with a T7-like genomic organization. Almost 65% of the predicted ORFs presented homology with proteins of the vibriophages Vc1 and phi-A318 infecting Vibrio cyclitrophicus and Vibrio alginolyticus, respectively. Phylogenetic analysis applying the amino acid sequence of the large terminase subunit confirmed the close relationship of these phages. Furthermore, the comparison of the RNA polymerase of these phages revealed that the motifs A, B and C related to the catalytic activity and the recognition loop related to promotor identification were also conserved. VEN has an obligate lytic life cycle demonstrated by experimental data and genomic analysis. These results suggest that VEN may provide a good candidate to control recurrent diseases caused by V. alginolyticus at HCMR. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.}, note = {Publisher: Springer Verlag}, keywords = {}, pubstate = {published}, tppubtype = {article} } Vibrio alginolyticus is a common marine bacterium implicated in disease outbreaks in marine farmed fish and invertebrates. Due to the inappropriate use of antibiotics in aquaculture, alternative therapies have been proposed. One of the most promising options is the use of lytic bacteriophages to control pathogenic bacteria. This work describes the isolation and characterization of a lytic phage (VEN) against a V. alginolyticus strain (V2) isolated from a disease outbreak in common dentex (Dentex dentex) cultured at the Hellenic Centre for Marine Research (HCMR) in Crete, Greece. The bacteriophage is morphologically similar to phages from Podoviridae family and remained stable for 1 year at 4 °C and over 1 h when kept at 50 °C. VEN was able to lyse the host bacteria at several multiplicity of infection (MOI) (0.1–100) in liquid cultures. However, it was unable to infect other V. alginolyticus strains. Its genome consists of 44,603 bp with a GC content of 43.5%, while sequence analysis revealed the presence of 54 potential ORFs with a T7-like genomic organization. Almost 65% of the predicted ORFs presented homology with proteins of the vibriophages Vc1 and phi-A318 infecting Vibrio cyclitrophicus and Vibrio alginolyticus, respectively. Phylogenetic analysis applying the amino acid sequence of the large terminase subunit confirmed the close relationship of these phages. Furthermore, the comparison of the RNA polymerase of these phages revealed that the motifs A, B and C related to the catalytic activity and the recognition loop related to promotor identification were also conserved. VEN has an obligate lytic life cycle demonstrated by experimental data and genomic analysis. These results suggest that VEN may provide a good candidate to control recurrent diseases caused by V. alginolyticus at HCMR. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature. |
Kalatzis, P G; Castillo, D; Katharios, P; Middelboe, M Bacteriophage interactions with marine pathogenic vibrios: Implications for phage therapy Journal Article Antibiotics, 7 (1), pp. 1–23, 2018, ISSN: 20796382, (Publisher: MDPI AG). @article{kalatzis_bacteriophage_2018, title = {Bacteriophage interactions with marine pathogenic vibrios: Implications for phage therapy}, author = {P G Kalatzis and D Castillo and P Katharios and M Middelboe}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85043227912&doi=10.3390%2fantibiotics7010015&partnerID=40&md5=c2609e35bdaa50623bfdd8dc492ee0e2}, doi = {10.3390/antibiotics7010015}, issn = {20796382}, year = {2018}, date = {2018-01-01}, journal = {Antibiotics}, volume = {7}, number = {1}, pages = {1--23}, abstract = {A global distribution in marine, brackish, and freshwater ecosystems, in combination with high abundances and biomass, make vibrios key players in aquatic environments, as well as important pathogens for humans and marine animals. Incidents of Vibrio-associated diseases (vibriosis) in marine aquaculture are being increasingly reported on a global scale, due to the fast growth of the industry over the past few decades years. The administration of antibiotics has been the most commonly applied therapy used to control vibriosis outbreaks, giving rise to concerns about development and spreading of antibiotic-resistant bacteria in the environment. Hence, the idea of using lytic bacteriophages as therapeutic agents against bacterial diseases has been revived during the last years. Bacteriophage therapy constitutes a promising alternative not only for treatment, but also for prevention of vibriosis in aquaculture. However, several scientific and technological challenges still need further investigation before reliable, reproducible treatments with commercial potential are available for the aquaculture industry. The potential and the challenges of phage-based alternatives to antibiotic treatment of vibriosis are addressed in this review. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.}, note = {Publisher: MDPI AG}, keywords = {}, pubstate = {published}, tppubtype = {article} } A global distribution in marine, brackish, and freshwater ecosystems, in combination with high abundances and biomass, make vibrios key players in aquatic environments, as well as important pathogens for humans and marine animals. Incidents of Vibrio-associated diseases (vibriosis) in marine aquaculture are being increasingly reported on a global scale, due to the fast growth of the industry over the past few decades years. The administration of antibiotics has been the most commonly applied therapy used to control vibriosis outbreaks, giving rise to concerns about development and spreading of antibiotic-resistant bacteria in the environment. Hence, the idea of using lytic bacteriophages as therapeutic agents against bacterial diseases has been revived during the last years. Bacteriophage therapy constitutes a promising alternative not only for treatment, but also for prevention of vibriosis in aquaculture. However, several scientific and technological challenges still need further investigation before reliable, reproducible treatments with commercial potential are available for the aquaculture industry. The potential and the challenges of phage-based alternatives to antibiotic treatment of vibriosis are addressed in this review. © 2018 by the authors. Licensee MDPI, Basel, Switzerland. |
Qi, W; Cascarano, M C; Schlapbach, R; Katharios, P; Vaughan, L; Seth-Smith, H M B Ca. Endozoicomonas cretensis: A novel fish pathogen characterized by genome plasticity Journal Article Genome Biology and Evolution, 10 (6), pp. 1363–1374, 2018, ISSN: 17596653, (Publisher: Oxford University Press). @article{qi_ca_2018, title = {Ca. Endozoicomonas cretensis: A novel fish pathogen characterized by genome plasticity}, author = {W Qi and M C Cascarano and R Schlapbach and P Katharios and L Vaughan and H M B Seth-Smith}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85052000776&doi=10.1093%2fgbe%2fevy092&partnerID=40&md5=804287f4e3358e9490133d60a8ae2e71}, doi = {10.1093/gbe/evy092}, issn = {17596653}, year = {2018}, date = {2018-01-01}, journal = {Genome Biology and Evolution}, volume = {10}, number = {6}, pages = {1363--1374}, abstract = {Endozoicomonas bacteria are generally beneficial symbionts of diverse marine invertebrates including reef-building corals, sponges, sea squirts, sea slugs, molluscs, and Bryozoans. In contrast, the recently reported Ca. Endozoicomonas cretensis was identified as a vertebrate pathogen, causing epitheliocystis in fish larvae resulting in massive mortality. Here, we described the Ca. E. cretensis draft genome, currently undergoing genome decay as evidenced by massive insertion sequence (IS element) expansion and pseudogene formation. Many of the insertion sequences are also predicted to carry outward-directed promoters, implying that they may be able to modulate the expression of neighbouring coding sequences (CDSs). Comparative genomic analysis has revealed many Ca. E. cretensis-specific CDSs, phage integration and novel gene families. Potential virulence related CDSs and machineries were identified in the genome, including secretion systems and related effector proteins, and systems related to biofilm formation and directed cell movement. Mucin degradation would be of importance to a fish pathogen, and many candidate CDSs associated with this pathway have been identified. The genome may reflect a bacteriumin the process of changing niche from symbiont to pathogen, through expansion of virulence genes and some loss of metabolic capacity. © The Author(s) 2018.}, note = {Publisher: Oxford University Press}, keywords = {}, pubstate = {published}, tppubtype = {article} } Endozoicomonas bacteria are generally beneficial symbionts of diverse marine invertebrates including reef-building corals, sponges, sea squirts, sea slugs, molluscs, and Bryozoans. In contrast, the recently reported Ca. Endozoicomonas cretensis was identified as a vertebrate pathogen, causing epitheliocystis in fish larvae resulting in massive mortality. Here, we described the Ca. E. cretensis draft genome, currently undergoing genome decay as evidenced by massive insertion sequence (IS element) expansion and pseudogene formation. Many of the insertion sequences are also predicted to carry outward-directed promoters, implying that they may be able to modulate the expression of neighbouring coding sequences (CDSs). Comparative genomic analysis has revealed many Ca. E. cretensis-specific CDSs, phage integration and novel gene families. Potential virulence related CDSs and machineries were identified in the genome, including secretion systems and related effector proteins, and systems related to biofilm formation and directed cell movement. Mucin degradation would be of importance to a fish pathogen, and many candidate CDSs associated with this pathway have been identified. The genome may reflect a bacteriumin the process of changing niche from symbiont to pathogen, through expansion of virulence genes and some loss of metabolic capacity. © The Author(s) 2018. |
Tsertou, M I; Smyrli, M; Kokkari, C; Antonopoulou, E; Katharios, P The aetiology of systemic granulomatosis in meagre (Argyrosomus regius): The “Nocardia” hypothesis Journal Article Aquaculture Reports, 12 , pp. 5–11, 2018, ISSN: 23525134, (Publisher: Elsevier B.V.). @article{tsertou_aetiology_2018, title = {The aetiology of systemic granulomatosis in meagre (Argyrosomus regius): The “Nocardia” hypothesis}, author = {M I Tsertou and M Smyrli and C Kokkari and E Antonopoulou and P Katharios}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85052467668&doi=10.1016%2fj.aqrep.2018.08.002&partnerID=40&md5=625a13223330d31956144851287ee794}, doi = {10.1016/j.aqrep.2018.08.002}, issn = {23525134}, year = {2018}, date = {2018-01-01}, journal = {Aquaculture Reports}, volume = {12}, pages = {5--11}, abstract = {One of the most important bottlenecks of meagre (Argyrosomus regius) production is Systemic Granulomatosis (SG), a pathological condition affecting the majority of farmed populations. The aetiology of the disease, which is characterized by multiple granulomas in all soft tissues, is unknown; however, two hypotheses have been raised. The first is that it may be a metabolic disorder similar to systemic granulomas observed in other cultured fishes. The second hypothesis is that it is caused by bacterial pathogens, most likely Nocardia spp. (i.e. nocardiosis). The aim of this study was to test the bacterial pathogen hypothesis as the aetiological factor of SG. Meagre from various locations of Greece were sampled and examined using microbiological, molecular and histological techniques. The prevalence of granulomas in fish was 100% and was not correlated to an infectious agent, whereas nocardiosis was found in a single incidence (prevalence: 1.3%). The results suggest that nocardiosis is not the cause of SG, as it is most probably present in a confined geographical region in Greece. The metabolic disorder hypothesis for SG is more probable and more research has to been done to conclusively identify the aetiological factor of SG. © 2018 The Authors}, note = {Publisher: Elsevier B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } One of the most important bottlenecks of meagre (Argyrosomus regius) production is Systemic Granulomatosis (SG), a pathological condition affecting the majority of farmed populations. The aetiology of the disease, which is characterized by multiple granulomas in all soft tissues, is unknown; however, two hypotheses have been raised. The first is that it may be a metabolic disorder similar to systemic granulomas observed in other cultured fishes. The second hypothesis is that it is caused by bacterial pathogens, most likely Nocardia spp. (i.e. nocardiosis). The aim of this study was to test the bacterial pathogen hypothesis as the aetiological factor of SG. Meagre from various locations of Greece were sampled and examined using microbiological, molecular and histological techniques. The prevalence of granulomas in fish was 100% and was not correlated to an infectious agent, whereas nocardiosis was found in a single incidence (prevalence: 1.3%). The results suggest that nocardiosis is not the cause of SG, as it is most probably present in a confined geographical region in Greece. The metabolic disorder hypothesis for SG is more probable and more research has to been done to conclusively identify the aetiological factor of SG. © 2018 The Authors |
2017 |
Seth-Smith, Helena M B; Katharios, Pantelis; Dourala, Nancy; Mateos, José M; Fehr, Alexander G J; Nufer, Lisbeth; Ruetten, Maja; Soto, Maricruz Guevara; Vaughan, Lloyd Frontiers in Microbiology, 8 , pp. 508, 2017, ISSN: 1664-302X. @article{seth-smith_ca_2017, title = {Ca. Similichlamydia in Epitheliocystis Co-infection of Gilthead Seabream Gills: Unique Morphological Features of a Deep Branching Chlamydial Family}, author = {Helena M B Seth-Smith and Pantelis Katharios and Nancy Dourala and José M Mateos and Alexander G J Fehr and Lisbeth Nufer and Maja Ruetten and Maricruz Guevara Soto and Lloyd Vaughan}, url = {http://journal.frontiersin.org/article/10.3389/fmicb.2017.00508/full}, doi = {10.3389/fmicb.2017.00508}, issn = {1664-302X}, year = {2017}, date = {2017-03-01}, urldate = {2020-09-09}, journal = {Frontiers in Microbiology}, volume = {8}, pages = {508}, abstract = {The Planctomycetes-Verrucomicrobia-Chlamydiae (PVC) bacterial superphylum constitutes a broad range of organisms with an intriguing array of ultrastructural morphologies, including intracellular membranes and compartments and their corresponding complex genomes encoding these forms. The phylum Chlamydiae are all obligate intracellular bacteria and, although much is already known of their genomes from various families and how these regulate the various morphological forms, we know remarkably little about what is likely the deepest rooting clade of this phylum, which has only been found to contain pathogens of marine and fresh water vertebrates. The disease they are associated with is called epitheliocystis; however, analyses of the causative agents is hindered by an inability to cultivate them for refined in vitro experimentation. For this reason, we have developed tools to analyse both the genomes and the ultrastructures of bacteria causing this disease, directly from infected tissues. Here we present structural data for a member of the family Ca. Similichlamydiaceae from this deep-rooted clade, which we have identified using molecular tools, in epitheliocystis lesions of gilthead seabream (Sparus aurata) in Greece. We present evidence that the chlamydial inclusions appear to develop in a perinuclear location, similar to other members of the phylum and that a chlamydial developmental cycle is present, with chlamydial forms similar to reticular bodies (RBs) and elementary bodies (EBs) detected. Division of the RBs appeared to follow a budding process, and larger RBs with multiple condensed nucleoids were detected using both transmission electron microscopy (TEM) and by focused-ion beam, scanning electron microscopy (FIB-SEM). As model hosts, fish offer many advantages for investigation, and we hope by these efforts to encourage others to explore the biology of fish pathogens from the PVC.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The Planctomycetes-Verrucomicrobia-Chlamydiae (PVC) bacterial superphylum constitutes a broad range of organisms with an intriguing array of ultrastructural morphologies, including intracellular membranes and compartments and their corresponding complex genomes encoding these forms. The phylum Chlamydiae are all obligate intracellular bacteria and, although much is already known of their genomes from various families and how these regulate the various morphological forms, we know remarkably little about what is likely the deepest rooting clade of this phylum, which has only been found to contain pathogens of marine and fresh water vertebrates. The disease they are associated with is called epitheliocystis; however, analyses of the causative agents is hindered by an inability to cultivate them for refined in vitro experimentation. For this reason, we have developed tools to analyse both the genomes and the ultrastructures of bacteria causing this disease, directly from infected tissues. Here we present structural data for a member of the family Ca. Similichlamydiaceae from this deep-rooted clade, which we have identified using molecular tools, in epitheliocystis lesions of gilthead seabream (Sparus aurata) in Greece. We present evidence that the chlamydial inclusions appear to develop in a perinuclear location, similar to other members of the phylum and that a chlamydial developmental cycle is present, with chlamydial forms similar to reticular bodies (RBs) and elementary bodies (EBs) detected. Division of the RBs appeared to follow a budding process, and larger RBs with multiple condensed nucleoids were detected using both transmission electron microscopy (TEM) and by focused-ion beam, scanning electron microscopy (FIB-SEM). As model hosts, fish offer many advantages for investigation, and we hope by these efforts to encourage others to explore the biology of fish pathogens from the PVC. |
Cascarano, M C; Keklikoglou, K; Arvanitidis, C; Katharios, P Zootaxa, 4337 (1), pp. 91–108, 2017, ISSN: 11755326, (Publisher: Magnolia Press). @article{cascarano_contribution_2017, title = {Contribution to the morphological description of the marine leech, Ozobranchus margoi (Apáthy) (Rhynchobdellida: Ozobranchidae)by using combined histology, micro-CT and SEM}, author = {M C Cascarano and K Keklikoglou and C Arvanitidis and P Katharios}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85031712662&doi=10.11646%2fzootaxa.4337.1.4&partnerID=40&md5=2af1dcba738b14946bfe874ad9b1eb88}, doi = {10.11646/zootaxa.4337.1.4}, issn = {11755326}, year = {2017}, date = {2017-01-01}, journal = {Zootaxa}, volume = {4337}, number = {1}, pages = {91--108}, abstract = {The ozobranchid leech, Ozobranchus margoi (Apáthy, 1890), parasite of the loggerhead turtle, Caretta caretta (Linnaeus), was described through a multilevel approach including three different techniques: scanning electronic microscopy, histology and micro-CT. New insights are provided concerning the structure of the sensilla on the body, the eyes with emphasis on the structure of photoreceptors, the digestive system and the reproductive organs. Copyright © 2017 Magnolia Press.}, note = {Publisher: Magnolia Press}, keywords = {}, pubstate = {published}, tppubtype = {article} } The ozobranchid leech, Ozobranchus margoi (Apáthy, 1890), parasite of the loggerhead turtle, Caretta caretta (Linnaeus), was described through a multilevel approach including three different techniques: scanning electronic microscopy, histology and micro-CT. New insights are provided concerning the structure of the sensilla on the body, the eyes with emphasis on the structure of photoreceptors, the digestive system and the reproductive organs. Copyright © 2017 Magnolia Press. |
Chatzikonstantinou, M; Kalliampakou, A; Gatzogia, M; Flemetakis, E; Katharios, P; Labrou, N E Comparative analyses and evaluation of the cosmeceutical potential of selected Chlorella strains Journal Article Journal of Applied Phycology, 29 (1), pp. 179–188, 2017, ISSN: 09218971, (Publisher: Springer Netherlands). @article{chatzikonstantinou_comparative_2017, title = {Comparative analyses and evaluation of the cosmeceutical potential of selected Chlorella strains}, author = {M Chatzikonstantinou and A Kalliampakou and M Gatzogia and E Flemetakis and P Katharios and N E Labrou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84979211606&doi=10.1007%2fs10811-016-0909-1&partnerID=40&md5=e4b187b06b870915a70691e0110e850a}, doi = {10.1007/s10811-016-0909-1}, issn = {09218971}, year = {2017}, date = {2017-01-01}, journal = {Journal of Applied Phycology}, volume = {29}, number = {1}, pages = {179--188}, abstract = {In the present study, aqueous extracts from eight Chlorella strains were evaluated towards selected cosmeceutical properties such as enzymatic and non-enzymatic antioxidant capacity, glutathione content, inhibition potency towards skin regulatory enzymes, and cytotoxic effect on human cell lines. The results showed that the Chlorella strains display wide catalytic diversity with different strains exhibiting quite varied antioxidant enzymatic activities. The enzymes glutathione reductase, peroxidase, and superoxide dismutase were found in all strains, whereas only Chlorella minutissima displays glutathione transferase activity. Total antioxidant capacity (TAC) measurements, using the FRAP, ABTS, and DPPH assays, indicated significant differences among strains suggesting a large chemical diversity of antioxidants in Chlorella species. Chlorella minutissima extract showed high inhibitory potency with IC50 23.3 ± 2.5 and 29.2±2.7 μg towards tyrosinase and elastase, respectively, suggesting a potential activity in controlling skin aging, inflammatory process, and pigmentation. Furthermore, the effect of C. minutissima extract on a human hepatoma cell line (Huh-7) was evaluated using cell proliferation assay and qRT-PCR to analyze the expression of marker genes (SOD1, GPx1, GPx2) involved in oxidative stress response. The results established the absence of cytotoxic effects of the aqueous extracts. SOD1, GPx1, and GPx4 expression levels showed a specific profile and appear to be slightly upregulated, downregulated, and remained unaffected, respectively. Taken together, these results demonstrate that Chlorella species can provide promising extracts rich in antioxidants, anti-aging, and skin-whitening ingredients with the potential for use as raw material in cosmetics industry. © 2016, Springer Science+Business Media Dordrecht.}, note = {Publisher: Springer Netherlands}, keywords = {}, pubstate = {published}, tppubtype = {article} } In the present study, aqueous extracts from eight Chlorella strains were evaluated towards selected cosmeceutical properties such as enzymatic and non-enzymatic antioxidant capacity, glutathione content, inhibition potency towards skin regulatory enzymes, and cytotoxic effect on human cell lines. The results showed that the Chlorella strains display wide catalytic diversity with different strains exhibiting quite varied antioxidant enzymatic activities. The enzymes glutathione reductase, peroxidase, and superoxide dismutase were found in all strains, whereas only Chlorella minutissima displays glutathione transferase activity. Total antioxidant capacity (TAC) measurements, using the FRAP, ABTS, and DPPH assays, indicated significant differences among strains suggesting a large chemical diversity of antioxidants in Chlorella species. Chlorella minutissima extract showed high inhibitory potency with IC50 23.3 ± 2.5 and 29.2±2.7 μg towards tyrosinase and elastase, respectively, suggesting a potential activity in controlling skin aging, inflammatory process, and pigmentation. Furthermore, the effect of C. minutissima extract on a human hepatoma cell line (Huh-7) was evaluated using cell proliferation assay and qRT-PCR to analyze the expression of marker genes (SOD1, GPx1, GPx2) involved in oxidative stress response. The results established the absence of cytotoxic effects of the aqueous extracts. SOD1, GPx1, and GPx4 expression levels showed a specific profile and appear to be slightly upregulated, downregulated, and remained unaffected, respectively. Taken together, these results demonstrate that Chlorella species can provide promising extracts rich in antioxidants, anti-aging, and skin-whitening ingredients with the potential for use as raw material in cosmetics industry. © 2016, Springer Science+Business Media Dordrecht. |
Kalatzis, P G; Rørbo, N; Castillo, D; Mauritzen, J J; Jørgensen, J; Kokkari, C; Zhang, F; Katharios, P; Middelboe, M Stumbling across the same phage: Comparative genomics of widespread temperate phages infecting the fish pathogen Vibrio anguillarum Journal Article Viruses, 9 (5), pp. 122, 2017, ISSN: 19994915, (Publisher: MDPI AG). @article{kalatzis_stumbling_2017, title = {Stumbling across the same phage: Comparative genomics of widespread temperate phages infecting the fish pathogen Vibrio anguillarum}, author = {P G Kalatzis and N Rørbo and D Castillo and J J Mauritzen and J Jørgensen and C Kokkari and F Zhang and P Katharios and M Middelboe}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85019581810&doi=10.3390%2fv9050122&partnerID=40&md5=a0f1b080ff39b0a1d1bc21f1692e1b58}, doi = {10.3390/v9050122}, issn = {19994915}, year = {2017}, date = {2017-01-01}, journal = {Viruses}, volume = {9}, number = {5}, pages = {122}, abstract = {Nineteen Vibrio anguillarum-specific temperate bacteriophages isolated across Europe and Chile from aquaculture and environmental sites were genome sequenced and analyzed for host range, morphology and life cycle characteristics. The phages were classified as Siphoviridae with genome sizes between 46,006 and 54,201 bp. All 19 phages showed high genetic similarity, and 13 phages were genetically identical. Apart from sporadically distributed single nucleotide polymorphisms (SNPs), genetic diversifications were located in three variable regions (VR1, VR2 and VR3) in six of the phage genomes. Identification of specific genes, such as N6-adenine methyltransferase and lambda like repressor, as well as the presence of a tRNAArg, suggested a both mutualistic and parasitic interaction between phages and hosts. During short term phage exposure experiments, 28% of a V. anguillarum host population was lysogenized by the temperate phages and a genomic analysis of a collection of 31 virulent V. anguillarum showed that the isolated phages were present as prophages in textgreater50% of the strains covering large geographical distances. Further, phage sequences were widely distributed among CRISPR-Cas arrays of publicly available sequenced Vibrios. The observed distribution of these specific temperate Vibriophages across large geographical scales may be explained by efficient dispersal of phages and bacteria in the marine environment combined with a mutualistic interaction between temperate phages and their hosts which selects for co-existence rather than arms race dynamics. © 2017 by the authors. Licensee MDPI, Basel, Switzerland.}, note = {Publisher: MDPI AG}, keywords = {}, pubstate = {published}, tppubtype = {article} } Nineteen Vibrio anguillarum-specific temperate bacteriophages isolated across Europe and Chile from aquaculture and environmental sites were genome sequenced and analyzed for host range, morphology and life cycle characteristics. The phages were classified as Siphoviridae with genome sizes between 46,006 and 54,201 bp. All 19 phages showed high genetic similarity, and 13 phages were genetically identical. Apart from sporadically distributed single nucleotide polymorphisms (SNPs), genetic diversifications were located in three variable regions (VR1, VR2 and VR3) in six of the phage genomes. Identification of specific genes, such as N6-adenine methyltransferase and lambda like repressor, as well as the presence of a tRNAArg, suggested a both mutualistic and parasitic interaction between phages and hosts. During short term phage exposure experiments, 28% of a V. anguillarum host population was lysogenized by the temperate phages and a genomic analysis of a collection of 31 virulent V. anguillarum showed that the isolated phages were present as prophages in textgreater50% of the strains covering large geographical distances. Further, phage sequences were widely distributed among CRISPR-Cas arrays of publicly available sequenced Vibrios. The observed distribution of these specific temperate Vibriophages across large geographical scales may be explained by efficient dispersal of phages and bacteria in the marine environment combined with a mutualistic interaction between temperate phages and their hosts which selects for co-existence rather than arms race dynamics. © 2017 by the authors. Licensee MDPI, Basel, Switzerland. |
Sterioti, A; Doxa, C C; Grigoriou, P; Vardanis, G; Cascarano, M C; Katharios, P Ozobranchus margoi Infections in Loggerhead Turtles (Caretta caretta) in Greece and Potential Treatment Options Journal Article Journal of Exotic Pet Medicine, 26 (3), pp. 196–199, 2017, ISSN: 15575063, (Publisher: W.B. Saunders). @article{sterioti_ozobranchus_2017, title = {Ozobranchus margoi Infections in Loggerhead Turtles (Caretta caretta) in Greece and Potential Treatment Options}, author = {A Sterioti and C C Doxa and P Grigoriou and G Vardanis and M C Cascarano and P Katharios}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85020130417&doi=10.1053%2fj.jepm.2017.05.006&partnerID=40&md5=df6cf6b579863fe0b8d0241ce8fa4b20}, doi = {10.1053/j.jepm.2017.05.006}, issn = {15575063}, year = {2017}, date = {2017-01-01}, journal = {Journal of Exotic Pet Medicine}, volume = {26}, number = {3}, pages = {196--199}, abstract = {Two injured loggerhead turtles, Caretta caretta, were presented to the public aquarium in Crete, infested by the parasitic marine leech, Ozobranchus margoi. This report describes these 2 loggerhead turtles and parasitic marine leeches along with the results of various treatment trials. Iodine ointment was the most efficient treatment against all life stages of O. margoi, taking into account animal welfare, ease of application, and efficacy. Monitoring the occurrence of parasitic leeches is important, as apart from the direct clinical affects to the host animal, they have been reported as vectors of threatening viral diseases. © 2017 Elsevier Inc.}, note = {Publisher: W.B. Saunders}, keywords = {}, pubstate = {published}, tppubtype = {article} } Two injured loggerhead turtles, Caretta caretta, were presented to the public aquarium in Crete, infested by the parasitic marine leech, Ozobranchus margoi. This report describes these 2 loggerhead turtles and parasitic marine leeches along with the results of various treatment trials. Iodine ointment was the most efficient treatment against all life stages of O. margoi, taking into account animal welfare, ease of application, and efficacy. Monitoring the occurrence of parasitic leeches is important, as apart from the direct clinical affects to the host animal, they have been reported as vectors of threatening viral diseases. © 2017 Elsevier Inc. |
Katharios, P Virulence regulation during late infection by a fish pathogen; sense and sensibility of bacteria may lead to novel vaccine development strategy Journal Article Virulence, 8 (7), pp. 1096–1098, 2017, ISSN: 21505594, (Publisher: Taylor and Francis Inc.). @article{katharios_virulence_2017, title = {Virulence regulation during late infection by a fish pathogen; sense and sensibility of bacteria may lead to novel vaccine development strategy}, author = {P Katharios}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85025132333&doi=10.1080%2f21505594.2017.1344807&partnerID=40&md5=6173ea428fe58dea83cde5687b680040}, doi = {10.1080/21505594.2017.1344807}, issn = {21505594}, year = {2017}, date = {2017-01-01}, journal = {Virulence}, volume = {8}, number = {7}, pages = {1096--1098}, note = {Publisher: Taylor and Francis Inc.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Katharios, P; Kalatzis, P G; Kokkari, C; Sarropoulou, E; Middelboe, M Isolation and characterization of a N4-like lytic bacteriophage infecting Vibrio splendidus, a pathogen of fish and bivalves Journal Article PLoS ONE, 12 (12), pp. e0190083, 2017, ISSN: 19326203, (Publisher: Public Library of Science). @article{katharios_isolation_2017, title = {Isolation and characterization of a N4-like lytic bacteriophage infecting Vibrio splendidus, a pathogen of fish and bivalves}, author = {P Katharios and P G Kalatzis and C Kokkari and E Sarropoulou and M Middelboe}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85039803769&doi=10.1371%2fjournal.pone.0190083&partnerID=40&md5=764cc6c742f2f2111527fd680e4aa5b2}, doi = {10.1371/journal.pone.0190083}, issn = {19326203}, year = {2017}, date = {2017-01-01}, journal = {PLoS ONE}, volume = {12}, number = {12}, pages = {e0190083}, abstract = {A novel virulent bacteriophage, vB_VspP_pVa5, infecting a strain of Vibrio splendidus was isolated from a sea-cage aquaculture farm in Greece, and characterized using microbiological methods and genomic analysis. Bacteriophage vB_VspP_pVa5 is a N4-like podovirus with an icosahedral head measuring 85 nm in length and a short non-contractile tail. The phage had a narrow host range infecting only the bacterial host, a latent period of 30 min and a burst size of 24 virions per infected bacterium. Its genome size was 78,145 bp and genomic analysis identified 107 densely-packed genes, 40 of which could be annotated. In addition to the very large virion encapsulated DNA-dependent RNA polymerase which is the signature of the N4-like genus, an interesting feature of the novel phage is the presence of a self-splicing group I intron in the thymidylate synthase gene. A tRNAStop interrupted by a 2.5kb open reading frame–containing area was also identified. The absence of genes related to lysogeny along with the high efficacy observed during in vitro cell lysis trials, indicate that the vB_VspP_pVa5 is a potential candidate component in a bacteriophage cocktail suitable for the biological control of V. splendidus in aquaculture. © 2017 Katharios et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.}, note = {Publisher: Public Library of Science}, keywords = {}, pubstate = {published}, tppubtype = {article} } A novel virulent bacteriophage, vB_VspP_pVa5, infecting a strain of Vibrio splendidus was isolated from a sea-cage aquaculture farm in Greece, and characterized using microbiological methods and genomic analysis. Bacteriophage vB_VspP_pVa5 is a N4-like podovirus with an icosahedral head measuring 85 nm in length and a short non-contractile tail. The phage had a narrow host range infecting only the bacterial host, a latent period of 30 min and a burst size of 24 virions per infected bacterium. Its genome size was 78,145 bp and genomic analysis identified 107 densely-packed genes, 40 of which could be annotated. In addition to the very large virion encapsulated DNA-dependent RNA polymerase which is the signature of the N4-like genus, an interesting feature of the novel phage is the presence of a self-splicing group I intron in the thymidylate synthase gene. A tRNAStop interrupted by a 2.5kb open reading frame–containing area was also identified. The absence of genes related to lysogeny along with the high efficacy observed during in vitro cell lysis trials, indicate that the vB_VspP_pVa5 is a potential candidate component in a bacteriophage cocktail suitable for the biological control of V. splendidus in aquaculture. © 2017 Katharios et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
Smyrli, M; Prapas, A; Rigos, G; Kokkari, C; Pavlidis, M; Katharios, P Fish Pathology, 52 (2), pp. 68–81, 2017, ISSN: 0388788X, (Publisher: Japanese Society of Fish Pathology). @article{smyrli_aeromonas_2017, title = {Aeromonas veronii infection associated with high morbidity and mortality in farmed European seabass Dicentrarchus labrax in the Aegean Sea, Greece}, author = {M Smyrli and A Prapas and G Rigos and C Kokkari and M Pavlidis and P Katharios}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85022184875&doi=10.3147%2fjsfp.52.68&partnerID=40&md5=d4e9a7e3342b0d17ae8291af235a0206}, doi = {10.3147/jsfp.52.68}, issn = {0388788X}, year = {2017}, date = {2017-01-01}, journal = {Fish Pathology}, volume = {52}, number = {2}, pages = {68--81}, abstract = {In the present study, we examined a disease caused by Aeromonas veronii bv. sobria in sea cage-farmed European seabass Dicentrarchus labrax, in the Aegean Sea, Greece. Commercial sized fish were affected by A. veronii bv. sobria and exhibited high morbidity and mortality. Gross pathologic features and histology revealed a systemic infection characterized by the presence of abscesses and chronic granulomatous inflammation. Two clinical bacterial isolates (Aero NS and Aero PDB) were identified as A. veronii bv. sobria based on bacteriological characteristics and sequence analysis for 16S rRNA and gyrB genes. Infectivity tests in the form of intraperitoneal injection administration (Aero NS) and immersion in a bacterial suspension (Aero NS and Aero PDB) revealed that both isolates could cause clinical signs similar to those observed in the field and high mortality rate. To our knowledge, this is the first report of A. veronii bv. sobria isolated from farmed European seabass in the Mediterranean Sea accompanied by supporting data of its pathogenicity. © 2017 The Japanese Society of Fish Pathology.}, note = {Publisher: Japanese Society of Fish Pathology}, keywords = {}, pubstate = {published}, tppubtype = {article} } In the present study, we examined a disease caused by Aeromonas veronii bv. sobria in sea cage-farmed European seabass Dicentrarchus labrax, in the Aegean Sea, Greece. Commercial sized fish were affected by A. veronii bv. sobria and exhibited high morbidity and mortality. Gross pathologic features and histology revealed a systemic infection characterized by the presence of abscesses and chronic granulomatous inflammation. Two clinical bacterial isolates (Aero NS and Aero PDB) were identified as A. veronii bv. sobria based on bacteriological characteristics and sequence analysis for 16S rRNA and gyrB genes. Infectivity tests in the form of intraperitoneal injection administration (Aero NS) and immersion in a bacterial suspension (Aero NS and Aero PDB) revealed that both isolates could cause clinical signs similar to those observed in the field and high mortality rate. To our knowledge, this is the first report of A. veronii bv. sobria isolated from farmed European seabass in the Mediterranean Sea accompanied by supporting data of its pathogenicity. © 2017 The Japanese Society of Fish Pathology. |
2016 |
Qi, W; Vaughan, L; Katharios, P; Schlapbach, R; Seth-Smith, H M B Genome Biology and Evolution, 8 (6), pp. 1672–1689, 2016, ISSN: 17596653, (Publisher: Oxford University Press). @article{qi_host-associated_2016, title = {Host-associated genomic features of the novel uncultured intracellular Pathogen Ca. Ichthyocystis revealed by direct sequencing of epitheliocysts}, author = {W Qi and L Vaughan and P Katharios and R Schlapbach and H M B Seth-Smith}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85014855085&doi=10.1093%2fgbe%2fevw111&partnerID=40&md5=e6a407680b87c77199ba72e7606682d8}, doi = {10.1093/gbe/evw111}, issn = {17596653}, year = {2016}, date = {2016-01-01}, journal = {Genome Biology and Evolution}, volume = {8}, number = {6}, pages = {1672--1689}, abstract = {Advances in single-cell and mini-metagenome sequencing have enabled important investigations into uncultured bacteria. In this study, we applied the mini-metagenome sequencing method to assemble genome drafts of the uncultured causative agents of epitheliocystis, an emerging infectious disease in theMediterranean aquaculture species gilthead seabream.We sequenced multiple cyst samples and constructed 11 genome drafts from a novel beta-proteobacterial lineage, Candidatus Ichthyocystis. The draft genomes demonstrate features typical of pathogenic bacteria with an obligate intracellular lifestyle: A reduced genome of up to 2.6Mb, reducedG+Ccontent, andreducedmetabolic capacity.Reconstruction ofmetabolic pathways reveals that Ca. Ichthyocystis genomes lack all amino acid synthesis pathways, compelling them to scavenge from the fish host. All genomes encode type II, III, and IV secretion systems, a large repertoire of predicted effectors, and a type IV pilus. These are all considered to be virulence factors, required for adherence, invasion, and host manipulation. However, no evidence of lipopolysaccharide synthesis could be found. Beyond the core functions shared within the genus, alignments showed distinction into different species, characterized by alternative large gene families. These comprise up to a third of each genome, appear to have arisen through duplication and diversification, encode many effector proteins, and are seemingly critical for virulence. Thus, Ca. Ichthyocystis represents a novel obligatory intracellular pathogenic beta-proteobacterial lineage. The methods used: Mini-metagenome analysis and manual annotation, have generated important insights into the lifestyle and evolution of the novel, uncultured pathogens, elucidating many putative virulence factors including an unprecedented array of novel gene families. © The Author 2016.}, note = {Publisher: Oxford University Press}, keywords = {}, pubstate = {published}, tppubtype = {article} } Advances in single-cell and mini-metagenome sequencing have enabled important investigations into uncultured bacteria. In this study, we applied the mini-metagenome sequencing method to assemble genome drafts of the uncultured causative agents of epitheliocystis, an emerging infectious disease in theMediterranean aquaculture species gilthead seabream.We sequenced multiple cyst samples and constructed 11 genome drafts from a novel beta-proteobacterial lineage, Candidatus Ichthyocystis. The draft genomes demonstrate features typical of pathogenic bacteria with an obligate intracellular lifestyle: A reduced genome of up to 2.6Mb, reducedG+Ccontent, andreducedmetabolic capacity.Reconstruction ofmetabolic pathways reveals that Ca. Ichthyocystis genomes lack all amino acid synthesis pathways, compelling them to scavenge from the fish host. All genomes encode type II, III, and IV secretion systems, a large repertoire of predicted effectors, and a type IV pilus. These are all considered to be virulence factors, required for adherence, invasion, and host manipulation. However, no evidence of lipopolysaccharide synthesis could be found. Beyond the core functions shared within the genus, alignments showed distinction into different species, characterized by alternative large gene families. These comprise up to a third of each genome, appear to have arisen through duplication and diversification, encode many effector proteins, and are seemingly critical for virulence. Thus, Ca. Ichthyocystis represents a novel obligatory intracellular pathogenic beta-proteobacterial lineage. The methods used: Mini-metagenome analysis and manual annotation, have generated important insights into the lifestyle and evolution of the novel, uncultured pathogens, elucidating many putative virulence factors including an unprecedented array of novel gene families. © The Author 2016. |
Skliros, D; Kalatzis, P G; Katharios, P; Flemetakis, E Comparative functional genomic analysis of two vibrio phages reveals complex metabolic interactions with the host cell Journal Article Frontiers in Microbiology, 7 , pp. 1807, 2016, ISSN: 1664302X, (Publisher: Frontiers Media S.A.). @article{skliros_comparative_2016, title = {Comparative functional genomic analysis of two vibrio phages reveals complex metabolic interactions with the host cell}, author = {D Skliros and P G Kalatzis and P Katharios and E Flemetakis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85006707461&doi=10.3389%2ffmicb.2016.01807&partnerID=40&md5=02cca0d66bd25f4fb040cd5ee07742d0}, doi = {10.3389/fmicb.2016.01807}, issn = {1664302X}, year = {2016}, date = {2016-01-01}, journal = {Frontiers in Microbiology}, volume = {7}, pages = {1807}, abstract = {Sequencing and annotation was performed for two large double stranded DNA bacteriophages, φGrn1 and φSt2 of the Myoviridae family, considered to be of great interest for phage therapy against Vibrios in aquaculture live feeds. In addition, phage-host metabolic interactions and exploitation was studied by transcript profiling of selected viral and host genes. Comparative genomic analysis with other large Vibrio phages was also performed to establish the presence and location of homing endonucleases highlighting distinct features for both phages. Phylogenetic analysis revealed that they belong to the "schizoT4like" clade. Although many reports of newly sequenced viruses have provided a large set of information, basic research related to the shift of the bacterial metabolism during infection remains stagnant. The function of many viral protein products in the process of infection is still unknown. Genome annotation identified the presence of several viral open reading frames (ORFs) participating in metabolism, including a Sir2/cobB (sirtuin) protein and a number of genes involved in auxiliary NAD+ and nucleotide biosynthesis, necessary for phage DNA replication. Key genes were subsequently selected for detail study of their expression levels during infection. This work suggests a complex metabolic interaction and exploitation of the host metabolic pathways and biochemical processes, including a possible post-translational protein modification, by the virus during infection. © 2016 Skliros, Kalatzis, Katharios and Flemetakis.}, note = {Publisher: Frontiers Media S.A.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Sequencing and annotation was performed for two large double stranded DNA bacteriophages, φGrn1 and φSt2 of the Myoviridae family, considered to be of great interest for phage therapy against Vibrios in aquaculture live feeds. In addition, phage-host metabolic interactions and exploitation was studied by transcript profiling of selected viral and host genes. Comparative genomic analysis with other large Vibrio phages was also performed to establish the presence and location of homing endonucleases highlighting distinct features for both phages. Phylogenetic analysis revealed that they belong to the "schizoT4like" clade. Although many reports of newly sequenced viruses have provided a large set of information, basic research related to the shift of the bacterial metabolism during infection remains stagnant. The function of many viral protein products in the process of infection is still unknown. Genome annotation identified the presence of several viral open reading frames (ORFs) participating in metabolism, including a Sir2/cobB (sirtuin) protein and a number of genes involved in auxiliary NAD+ and nucleotide biosynthesis, necessary for phage DNA replication. Key genes were subsequently selected for detail study of their expression levels during infection. This work suggests a complex metabolic interaction and exploitation of the host metabolic pathways and biochemical processes, including a possible post-translational protein modification, by the virus during infection. © 2016 Skliros, Kalatzis, Katharios and Flemetakis. |
Kalatzis, P G; Bastías, R; Kokkari, C; Katharios, P PLoS ONE, 11 (3), 2016, ISSN: 19326203, (Publisher: Public Library of Science). @article{kalatzis_isolation_2016, title = {Isolation and characterization of two lytic bacteriophages, φst2 and φgrn1; Phage therapy application for biological control of vibrio alginolyticus in aquaculture live feeds}, author = {P G Kalatzis and R Bastías and C Kokkari and P Katharios}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84961152351&doi=10.1371%2fjournal.pone.0151101&partnerID=40&md5=3cd378d8fe59cedc364f9f85f58851ef}, doi = {10.1371/journal.pone.0151101}, issn = {19326203}, year = {2016}, date = {2016-01-01}, journal = {PLoS ONE}, volume = {11}, number = {3}, abstract = {Bacterial infections are a serious problem in aquaculture since they can result in massive mortalities in farmed fish and invertebrates. Vibriosis is one of the most common diseases in marine aquaculture hatcheries and its causative agents are bacteria of the genus Vibrio mostly entering larval rearing water through live feeds, such as Artemia and rotifers. The pathogenic Vibrio alginolyticus strain V1, isolated during a vibriosis outbreak in cultured seabream, Sparus aurata, was used as host to isolate and characterize the two novel bacteriophages φSt2 and φGrn1 for phage therapy application. In vitro cell lysis experiments were performed against the bacterial host V. alginolyticus strain V1 but also against 12 presumptive Vibrio strains originating from live prey Artemia salina cultures indicating the strong lytic efficacy of the 2 phages. In vivo administration of the phage cocktail, φSt2 and φGrn1, at MOI = 100 directly on live prey A. salina cultures, led to a 93% decrease of presumptive Vibrio population after 4 h of treatment. Current study suggests that administration of φSt2 and φGrn1 to live preys could selectively reduce Vibrio load in fish hatcheries. Innovative and environmental friendly solutions against bacterial diseases are more than necessary and phage therapy is one of them. © 2016 Kalatzis et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.}, note = {Publisher: Public Library of Science}, keywords = {}, pubstate = {published}, tppubtype = {article} } Bacterial infections are a serious problem in aquaculture since they can result in massive mortalities in farmed fish and invertebrates. Vibriosis is one of the most common diseases in marine aquaculture hatcheries and its causative agents are bacteria of the genus Vibrio mostly entering larval rearing water through live feeds, such as Artemia and rotifers. The pathogenic Vibrio alginolyticus strain V1, isolated during a vibriosis outbreak in cultured seabream, Sparus aurata, was used as host to isolate and characterize the two novel bacteriophages φSt2 and φGrn1 for phage therapy application. In vitro cell lysis experiments were performed against the bacterial host V. alginolyticus strain V1 but also against 12 presumptive Vibrio strains originating from live prey Artemia salina cultures indicating the strong lytic efficacy of the 2 phages. In vivo administration of the phage cocktail, φSt2 and φGrn1, at MOI = 100 directly on live prey A. salina cultures, led to a 93% decrease of presumptive Vibrio population after 4 h of treatment. Current study suggests that administration of φSt2 and φGrn1 to live preys could selectively reduce Vibrio load in fish hatcheries. Innovative and environmental friendly solutions against bacterial diseases are more than necessary and phage therapy is one of them. © 2016 Kalatzis et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
Seth-Smith, H M B; Dourala, N; Fehr, A; Qi, W; Katharios, P; Ruetten, M; Mateos, J M; Nufer, L; Weilenmann, R; Ziegler, U; Thomson, N R; Schlapbach, R; Vaughan, L Emerging pathogens of gilthead seabream: characterisation and genomic analysis of novel intracellular β-proteobacteria Journal Article ISME Journal, 10 (7), pp. 1791–1803, 2016, ISSN: 17517362, (Publisher: Nature Publishing Group). @article{seth-smith_emerging_2016, title = {Emerging pathogens of gilthead seabream: characterisation and genomic analysis of novel intracellular β-proteobacteria}, author = {H M B Seth-Smith and N Dourala and A Fehr and W Qi and P Katharios and M Ruetten and J M Mateos and L Nufer and R Weilenmann and U Ziegler and N R Thomson and R Schlapbach and L Vaughan}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84957661023&doi=10.1038%2fismej.2015.223&partnerID=40&md5=8baa5be9708000d32194fb3b0217bf19}, doi = {10.1038/ismej.2015.223}, issn = {17517362}, year = {2016}, date = {2016-01-01}, journal = {ISME Journal}, volume = {10}, number = {7}, pages = {1791--1803}, abstract = {New and emerging environmental pathogens pose some of the greatest threats to modern aquaculture, a critical source of food protein globally. As with other intensive farming practices, increasing our understanding of the biology of infections is important to improve animal welfare and husbandry. The gill infection epitheliocystis is increasingly problematic in gilthead seabream (Sparus aurata), a major Mediterranean aquaculture species. Epitheliocystis is generally associated with chlamydial bacteria, yet we were not able to localise chlamydial targets within the major gilthead seabream lesions. Two previously unidentified species within a novel β-proteobacterial genus were instead identified. These co-infecting intracellular bacteria have been characterised using high-resolution imaging and genomics, presenting the most comprehensive study on epitheliocystis agents to date. Draft genomes of the two uncultured species, Ca. Ichthyocystis hellenicum and Ca. Ichthyocystis sparus, have been de novo sequenced and annotated from preserved material. Analysis of the genomes shows a compact core indicating a metabolic dependency on the host, and an accessory genome with an unprecedented number of tandemly arrayed gene families. This study represents a critical insight into novel, emerging fish pathogens and will be used to underpin future investigations into the bacterial origins, and to develop diagnostic and treatment strategies. © 2016 International Society for Microbial Ecology.}, note = {Publisher: Nature Publishing Group}, keywords = {}, pubstate = {published}, tppubtype = {article} } New and emerging environmental pathogens pose some of the greatest threats to modern aquaculture, a critical source of food protein globally. As with other intensive farming practices, increasing our understanding of the biology of infections is important to improve animal welfare and husbandry. The gill infection epitheliocystis is increasingly problematic in gilthead seabream (Sparus aurata), a major Mediterranean aquaculture species. Epitheliocystis is generally associated with chlamydial bacteria, yet we were not able to localise chlamydial targets within the major gilthead seabream lesions. Two previously unidentified species within a novel β-proteobacterial genus were instead identified. These co-infecting intracellular bacteria have been characterised using high-resolution imaging and genomics, presenting the most comprehensive study on epitheliocystis agents to date. Draft genomes of the two uncultured species, Ca. Ichthyocystis hellenicum and Ca. Ichthyocystis sparus, have been de novo sequenced and annotated from preserved material. Analysis of the genomes shows a compact core indicating a metabolic dependency on the host, and an accessory genome with an unprecedented number of tandemly arrayed gene families. This study represents a critical insight into novel, emerging fish pathogens and will be used to underpin future investigations into the bacterial origins, and to develop diagnostic and treatment strategies. © 2016 International Society for Microbial Ecology. |
Andree, K B; Roque, A; Duncan, N; Gisbert, E; Estevez, A; Tsertou, M I; Katharios, P Veterinary Parasitology: Regional Studies and Reports, 1-2 , pp. 75–79, 2016, (Publisher: Elsevier B.V.). @article{andree_diplectanum_2016, title = {Diplectanum sciaenae (Van Beneden & Hesse, 1863) (Monogenea) infecting meagre, Argyrosomus regius (Asso, 1801) broodstock in Catalonia, Spain. A case report}, author = {K B Andree and A Roque and N Duncan and E Gisbert and A Estevez and M I Tsertou and P Katharios}, url = {https://doi.org/10.1016/j.vprsr.2016.02.006}, doi = {10.1016/j.vprsr.2016.02.006}, year = {2016}, date = {2016-01-01}, journal = {Veterinary Parasitology: Regional Studies and Reports}, volume = {1-2}, pages = {75--79}, abstract = {Recurrent infection of captive meagre, Argyrosomus regius, by a monogenean parasite has been observed in the broodstock facilities of Institut de Recerca i Tecnologia Agroalimentàries (IRTA) in Catalonia, Spain, between 2008 and 2015. Following handling procedures related with hormonal treatment for spawning induction, one fish died. Post-mortem examination and detailed microscopical examination using light microscopy, SEM, and histology revealed intense infection by Diplectanum sciaenae (Monogenea, Diplectanidae) which caused noteworthy gill pathology. In the present study, we provide detailed description of the parasite and the pathology caused to its host. This is the first report associating this parasite with disease and mortality in farmed meagre.}, note = {Publisher: Elsevier B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Recurrent infection of captive meagre, Argyrosomus regius, by a monogenean parasite has been observed in the broodstock facilities of Institut de Recerca i Tecnologia Agroalimentàries (IRTA) in Catalonia, Spain, between 2008 and 2015. Following handling procedures related with hormonal treatment for spawning induction, one fish died. Post-mortem examination and detailed microscopical examination using light microscopy, SEM, and histology revealed intense infection by Diplectanum sciaenae (Monogenea, Diplectanidae) which caused noteworthy gill pathology. In the present study, we provide detailed description of the parasite and the pathology caused to its host. This is the first report associating this parasite with disease and mortality in farmed meagre. |
2015 |
Castillo, D; D'Alvise, P; Middelboe, M; Gram, L; Liu, S; Kalatzis, P G; Kokkari, C; Katharios, P Draft genome sequences of the fish pathogen Vibrio harveyi strains VH2 and VH5 Journal Article Genome Announcements, 3 (5), pp. e01062–15, 2015, ISSN: 21698287, (Publisher: American Society for Microbiology). @article{castillo_draft_2015, title = {Draft genome sequences of the fish pathogen Vibrio harveyi strains VH2 and VH5}, author = {D Castillo and P D'Alvise and M Middelboe and L Gram and S Liu and P G Kalatzis and C Kokkari and P Katharios}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85008929958&doi=10.1128%2fgenomeA.01062-15&partnerID=40&md5=2dfb9decf27ca3d6752ac7331683d3f3}, doi = {10.1128/genomeA.01062-15}, issn = {21698287}, year = {2015}, date = {2015-01-01}, journal = {Genome Announcements}, volume = {3}, number = {5}, pages = {e01062--15}, abstract = {Vibrio harveyi is an important marine pathogen that is responsible for vibriosis outbreaks in cultured fish and invertebrates worldwide. Here, we announce the draft genome sequences of V. harveyi strains VH2 and VH5, isolated from farmed juvenile Seriola dumerili during outbreaks of vibriosis in Crete, Greece. © 2015 Castillo et al.}, note = {Publisher: American Society for Microbiology}, keywords = {}, pubstate = {published}, tppubtype = {article} } Vibrio harveyi is an important marine pathogen that is responsible for vibriosis outbreaks in cultured fish and invertebrates worldwide. Here, we announce the draft genome sequences of V. harveyi strains VH2 and VH5, isolated from farmed juvenile Seriola dumerili during outbreaks of vibriosis in Crete, Greece. © 2015 Castillo et al. |
Kalatzis, P G; Kokkari, C; Katharios, P Systematic Parasitology, 92 (3), pp. 211–218, 2015, ISSN: 01655752, (Publisher: Kluwer Academic Publishers). @article{kalatzis_morphological_2015, title = {Morphological characterisation and phylogenetic relationships of Zschokkella candia n. sp. from the gall-bladder of Sparisoma cretense (L.) (Perciformes: Scaridae) in the Sea of Crete off Greece}, author = {P G Kalatzis and C Kokkari and P Katharios}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84943234121&doi=10.1007%2fs11230-015-9594-8&partnerID=40&md5=e43e7892cbdaf98c88dcb5f221b2f54f}, doi = {10.1007/s11230-015-9594-8}, issn = {01655752}, year = {2015}, date = {2015-01-01}, journal = {Systematic Parasitology}, volume = {92}, number = {3}, pages = {211--218}, abstract = {A new myxosporean parasite, Zschokkella candia n. sp., from the gall-bladder of the wild parrotfish Sparisoma cretense (L.) (Perciformes: Scaridae) is described based on light and scanning electron microscopy. Mature spores are elliptical, with mean dimensions 11.2 ± 0.5 × 7.8 ± 0.1 μm and possess spherical polar capsules with mean diameter of 2.3 ± 0.3 μm. The new species is differentiated from other similar species of the genus based on spore morphology, its coelozoic life-style and molecular data. The phylogenetic tree constructed using maximum likelihood analysis of small subunit (SSU) rDNA sequence data complements the characterisation of Zschokkella candia n. sp. by defining its phylogenetic position among the species of Zschokkella Auerbach, 1909 sequenced to date. The phylogenetic analysis supports the existing knowledge on the complicated polyphyletic relationships among the members of the genus Zschokkella. © 2015, Springer Science+Business Media Dordrecht.}, note = {Publisher: Kluwer Academic Publishers}, keywords = {}, pubstate = {published}, tppubtype = {article} } A new myxosporean parasite, Zschokkella candia n. sp., from the gall-bladder of the wild parrotfish Sparisoma cretense (L.) (Perciformes: Scaridae) is described based on light and scanning electron microscopy. Mature spores are elliptical, with mean dimensions 11.2 ± 0.5 × 7.8 ± 0.1 μm and possess spherical polar capsules with mean diameter of 2.3 ± 0.3 μm. The new species is differentiated from other similar species of the genus based on spore morphology, its coelozoic life-style and molecular data. The phylogenetic tree constructed using maximum likelihood analysis of small subunit (SSU) rDNA sequence data complements the characterisation of Zschokkella candia n. sp. by defining its phylogenetic position among the species of Zschokkella Auerbach, 1909 sequenced to date. The phylogenetic analysis supports the existing knowledge on the complicated polyphyletic relationships among the members of the genus Zschokkella. © 2015, Springer Science+Business Media Dordrecht. |
Castillo, D; D'Alvise, P; Kalatzis, P G; Kokkari, C; Middelboe, M; Gram, L; Liu, S; Katharios, P Draft genome sequences of Vibrio alginolyticus strains V1 and V2, opportunistic marine pathogens Journal Article Genome Announcements, 3 (4), pp. e00729–15, 2015, ISSN: 21698287, (Publisher: American Society for Microbiology). @article{castillo_draft_2015-1, title = {Draft genome sequences of Vibrio alginolyticus strains V1 and V2, opportunistic marine pathogens}, author = {D Castillo and P D'Alvise and P G Kalatzis and C Kokkari and M Middelboe and L Gram and S Liu and P Katharios}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85008429014&doi=10.1128%2fgenomeA.00729-15&partnerID=40&md5=52c4090ca04c4acf8107d2bbde043ddc}, doi = {10.1128/genomeA.00729-15}, issn = {21698287}, year = {2015}, date = {2015-01-01}, journal = {Genome Announcements}, volume = {3}, number = {4}, pages = {e00729--15}, abstract = {We announce the draft genome sequences of Vibrio alginolyticus strains V1 and V2, isolated from juvenile Sparus aurata and Dentex dentex, respectively, during outbreaks of vibriosis. The genome sequences are 5,257,950 bp with a G+C content of 44.5% for V. alginolyticus V1 and 5,068,299 bp with a G+C content of 44.8% for strain V2. These genomes provide further insights into the putative virulence factors, prophage carriage, and evolution of this opportunistic marine pathogen. © 2015 Castillo et al.}, note = {Publisher: American Society for Microbiology}, keywords = {}, pubstate = {published}, tppubtype = {article} } We announce the draft genome sequences of Vibrio alginolyticus strains V1 and V2, isolated from juvenile Sparus aurata and Dentex dentex, respectively, during outbreaks of vibriosis. The genome sequences are 5,257,950 bp with a G+C content of 44.5% for V. alginolyticus V1 and 5,068,299 bp with a G+C content of 44.8% for strain V2. These genomes provide further insights into the putative virulence factors, prophage carriage, and evolution of this opportunistic marine pathogen. © 2015 Castillo et al. |
Castillo, D; Jun, J W; D'Alvise, P; Middelboe, M; Gram, L; Liu, S; Katharios, P Draft genome sequence of Vibrio parahaemolyticus VH3, isolated from an aquaculture environment in Greece Journal Article Genome Announcements, 3 (4), pp. e00731, 2015, ISSN: 21698287, (Publisher: American Society for Microbiology). @article{castillo_draft_2015-2, title = {Draft genome sequence of Vibrio parahaemolyticus VH3, isolated from an aquaculture environment in Greece}, author = {D Castillo and J W Jun and P D'Alvise and M Middelboe and L Gram and S Liu and P Katharios}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85008430480&doi=10.1128%2fgenomeA.00731-15&partnerID=40&md5=43e474e8b8d60d6ca59adbf4f6255ae6}, doi = {10.1128/genomeA.00731-15}, issn = {21698287}, year = {2015}, date = {2015-01-01}, journal = {Genome Announcements}, volume = {3}, number = {4}, pages = {e00731}, abstract = {Vibrio parahaemolyticus is an important foodborne pathogen responsible for gastroenteritis outbreaks globally. It has also been identified as an important pathogen in aquatic organisms. Here, we report a draft genome sequence of V. parahaemolyticus, strain VH3, isolated from farmed juvenile greater amberjack, Seriola dumerili, in Greece. © 2015 Castillo et al.}, note = {Publisher: American Society for Microbiology}, keywords = {}, pubstate = {published}, tppubtype = {article} } Vibrio parahaemolyticus is an important foodborne pathogen responsible for gastroenteritis outbreaks globally. It has also been identified as an important pathogen in aquatic organisms. Here, we report a draft genome sequence of V. parahaemolyticus, strain VH3, isolated from farmed juvenile greater amberjack, Seriola dumerili, in Greece. © 2015 Castillo et al. |
Katharios, P; Kokkari, C; Dourala, N; Smyrli, M First report of Edwardsiellosis in cage-cultured sharpsnout sea bream, Diplodus puntazzo from the Mediterranean Journal Article BMC Veterinary Research, 11 (1), pp. 155, 2015, ISSN: 17466148, (Publisher: BioMed Central Ltd.). @article{katharios_first_2015, title = {First report of Edwardsiellosis in cage-cultured sharpsnout sea bream, Diplodus puntazzo from the Mediterranean}, author = {P Katharios and C Kokkari and N Dourala and M Smyrli}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84937551746&doi=10.1186%2fs12917-015-0482-x&partnerID=40&md5=a7c848edbc8a8ac0151f14734aabb8cf}, doi = {10.1186/s12917-015-0482-x}, issn = {17466148}, year = {2015}, date = {2015-01-01}, journal = {BMC Veterinary Research}, volume = {11}, number = {1}, pages = {155}, abstract = {Background: Edwardsiella tarda, is a serious bacterial pathogen affecting a broad range of aquaculture fish species. The bacterium has also been reported as a human pathogen, however recent studies have dissociated the fish pathogenic Edwardsiella from those isolated from humans by placing them in a new species, E. piscicida. Here we report the first case of Edwardsiellosis in cultured sharpsnout sea breams, Diplodus puntazzo in Greece. Case presentation: The disease has affected cultured sharpsnout sea breams of a commercial fish farm in a single location in East Greece. Two populations of sharpsnout sea breams stocked in two consecutive years in floating cages presented signs of disease which included nodules and abscesses in spleen and kidney, morbidity and cumulative mortality reaching 5.3 %. Using microbiological, biochemical and molecular tools we have identified Edwardsiella sp. as the main aetiological factor of the disease. Following phylogenetic analysis the bacterial isolates are grouped with the newly described Edwardsiella piscicida species. Conclusions: This is the first report of Edwardsiellosis in this species but most importantly in sea cage-cultured fish in the Mediterranean which may pose a serious threat for aquaculture fish species in this region. © 2015 Katharios et al.}, note = {Publisher: BioMed Central Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Background: Edwardsiella tarda, is a serious bacterial pathogen affecting a broad range of aquaculture fish species. The bacterium has also been reported as a human pathogen, however recent studies have dissociated the fish pathogenic Edwardsiella from those isolated from humans by placing them in a new species, E. piscicida. Here we report the first case of Edwardsiellosis in cultured sharpsnout sea breams, Diplodus puntazzo in Greece. Case presentation: The disease has affected cultured sharpsnout sea breams of a commercial fish farm in a single location in East Greece. Two populations of sharpsnout sea breams stocked in two consecutive years in floating cages presented signs of disease which included nodules and abscesses in spleen and kidney, morbidity and cumulative mortality reaching 5.3 %. Using microbiological, biochemical and molecular tools we have identified Edwardsiella sp. as the main aetiological factor of the disease. Following phylogenetic analysis the bacterial isolates are grouped with the newly described Edwardsiella piscicida species. Conclusions: This is the first report of Edwardsiellosis in this species but most importantly in sea cage-cultured fish in the Mediterranean which may pose a serious threat for aquaculture fish species in this region. © 2015 Katharios et al. |
Yáñez, R; Bastías, R; Higuera, G; Salgado, O; Katharios, P; Romero, J; Espejo, R; García, K Amplification of tlh gene in other Vibrionaceae specie by specie-specific multiplex PCR of Vibrio parahaemolyticus Journal Article Electronic Journal of Biotechnology, 18 (6), pp. 459–463, 2015, ISSN: 07173458, (Publisher: Electronic Journal of Biotechnology). @article{yanez_amplification_2015, title = {Amplification of tlh gene in other Vibrionaceae specie by specie-specific multiplex PCR of Vibrio parahaemolyticus}, author = {R Yáñez and R Bastías and G Higuera and O Salgado and P Katharios and J Romero and R Espejo and K García}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84946922086&doi=10.1016%2fj.ejbt.2015.09.007&partnerID=40&md5=df2aa530602f2f9b6fd76f3297e2c232}, doi = {10.1016/j.ejbt.2015.09.007}, issn = {07173458}, year = {2015}, date = {2015-01-01}, journal = {Electronic Journal of Biotechnology}, volume = {18}, number = {6}, pages = {459--463}, abstract = {Background: The surveillance of Vibrio parahaemolyticus in the Chilean coast has been mainly performed by multiplex PCR amplification of three different hemolysin genes, which are specie-specific virulence factors. These genes are also employed in the determination of V. parahaemolyticus pathogenic load in seafood and for characterization of pathogenic strains associated to diarrhea cases in human. During environmental surveillance that we performed every summer, we occasionally observed a thermolabile hemolysin (tlh) PCR product of a slightly smaller size than expected, which was coincident with low loads of V. parahaemolyticus in the environment. In order to understand this observation, we probed the specificity of tlh primers for detection of V. parahaemolyticus at different bacterial loads and DNA concentrations. Results: Primers used for detection of V. parahaemolyticus specific tlh amplified a slightly smaller tlh gene, which is found in Vibrio alginolyticus and other related strains. These amplicons were observed when V. parahaemolyticus was absent or in undetectable loads in the environment. Conclusions: Surveillance of V. parahaemolyticus using tlh primers can be imprecise because amplification of a V. parahaemolyticus specific marker in V. alginolyticus and other related strains occurs. This situation complicates potentially the estimation of bacterial load in seafood, because do not ensure the correct identification of V. parahaemolyticus when his load is low. Additionally, it could complicate the tracking of outbreaks of V. parahaemolyticus infections, considering the genetic markers used would not be specie-specific. © 2015 Pontificia Universidad Católica de Valparaíso. Production and hosting by Elsevier B.V. All rights reserved.}, note = {Publisher: Electronic Journal of Biotechnology}, keywords = {}, pubstate = {published}, tppubtype = {article} } Background: The surveillance of Vibrio parahaemolyticus in the Chilean coast has been mainly performed by multiplex PCR amplification of three different hemolysin genes, which are specie-specific virulence factors. These genes are also employed in the determination of V. parahaemolyticus pathogenic load in seafood and for characterization of pathogenic strains associated to diarrhea cases in human. During environmental surveillance that we performed every summer, we occasionally observed a thermolabile hemolysin (tlh) PCR product of a slightly smaller size than expected, which was coincident with low loads of V. parahaemolyticus in the environment. In order to understand this observation, we probed the specificity of tlh primers for detection of V. parahaemolyticus at different bacterial loads and DNA concentrations. Results: Primers used for detection of V. parahaemolyticus specific tlh amplified a slightly smaller tlh gene, which is found in Vibrio alginolyticus and other related strains. These amplicons were observed when V. parahaemolyticus was absent or in undetectable loads in the environment. Conclusions: Surveillance of V. parahaemolyticus using tlh primers can be imprecise because amplification of a V. parahaemolyticus specific marker in V. alginolyticus and other related strains occurs. This situation complicates potentially the estimation of bacterial load in seafood, because do not ensure the correct identification of V. parahaemolyticus when his load is low. Additionally, it could complicate the tracking of outbreaks of V. parahaemolyticus infections, considering the genetic markers used would not be specie-specific. © 2015 Pontificia Universidad Católica de Valparaíso. Production and hosting by Elsevier B.V. All rights reserved. |
Katharios, P; Seth-Smith, H M B; Fehr, A; Mateos, J M; Qi, W; Richter, D; Nufer, L; Ruetten, M; Soto, Guevara M; Ziegler, U; Thomson, N R; Schlapbach, R; Vaughan, L Scientific Reports, 5 , pp. 17609, 2015, ISSN: 20452322, (Publisher: Nature Publishing Group). @article{katharios_environmental_2015, title = {Environmental marine pathogen isolation using mesocosm culture of sharpsnout seabream: Striking genomic and morphological features of novel Endozoicomonas sp.}, author = {P Katharios and H M B Seth-Smith and A Fehr and J M Mateos and W Qi and D Richter and L Nufer and M Ruetten and M Guevara Soto and U Ziegler and N R Thomson and R Schlapbach and L Vaughan}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84949517859&doi=10.1038%2fsrep17609&partnerID=40&md5=f8b04acf96f393c71066cebfca267705}, doi = {10.1038/srep17609}, issn = {20452322}, year = {2015}, date = {2015-01-01}, journal = {Scientific Reports}, volume = {5}, pages = {17609}, abstract = {Aquaculture is a burgeoning industry, requiring diversification into new farmed species, which are often at risk from infectious disease. We used a mesocosm technique to investigate the susceptibility of sharpsnout seabream (Diplodus puntazzo) larvae to potential environmental pathogens in seawater compared to control borehole water. Fish exposed to seawater succumbed to epitheliocystis from 21 days post hatching, causing mortality in a quarter of the hosts. The pathogen responsible was not chlamydial, as is often found in epitheliocystis, but a novel species of the γ-proteobacterial genus Endozoicomonas. Detailed characterisation of this pathogen within the infectious lesions using high resolution fluorescent and electron microscopy showed densely packed rod shaped bacteria. A draft genome sequence of this uncultured bacterium was obtained from preserved material. Comparison with the genome of the Endozoicomonas elysicola type strain shows that the genome of Ca. Endozoicomonas cretensis is undergoing decay through loss of functional genes and insertion sequence expansion, often indicative of adaptation to a new niche or restriction to an alternative lifestyle. These results demonstrate the advantage of mesocosm studies for investigating the effect of environmental bacteria on susceptible hosts and provide an important insight into the genome dynamics of a novel fish pathogen.}, note = {Publisher: Nature Publishing Group}, keywords = {}, pubstate = {published}, tppubtype = {article} } Aquaculture is a burgeoning industry, requiring diversification into new farmed species, which are often at risk from infectious disease. We used a mesocosm technique to investigate the susceptibility of sharpsnout seabream (Diplodus puntazzo) larvae to potential environmental pathogens in seawater compared to control borehole water. Fish exposed to seawater succumbed to epitheliocystis from 21 days post hatching, causing mortality in a quarter of the hosts. The pathogen responsible was not chlamydial, as is often found in epitheliocystis, but a novel species of the γ-proteobacterial genus Endozoicomonas. Detailed characterisation of this pathogen within the infectious lesions using high resolution fluorescent and electron microscopy showed densely packed rod shaped bacteria. A draft genome sequence of this uncultured bacterium was obtained from preserved material. Comparison with the genome of the Endozoicomonas elysicola type strain shows that the genome of Ca. Endozoicomonas cretensis is undergoing decay through loss of functional genes and insertion sequence expansion, often indicative of adaptation to a new niche or restriction to an alternative lifestyle. These results demonstrate the advantage of mesocosm studies for investigating the effect of environmental bacteria on susceptible hosts and provide an important insight into the genome dynamics of a novel fish pathogen. |
2014 |
Katharios, P; Kokkari, C; Sterioti, A; Smyrli, M; Kalatzis, P G Enteromyxum leei infection in parrotfish, Sparisoma cretense: Histopathological, morphological and molecular study Journal Article Veterinary Parasitology, 199 (3-4), pp. 136–143, 2014, ISSN: 03044017. @article{katharios_enteromyxum_2014, title = {Enteromyxum leei infection in parrotfish, Sparisoma cretense: Histopathological, morphological and molecular study}, author = {P Katharios and C Kokkari and A Sterioti and M Smyrli and P G Kalatzis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84890553160&doi=10.1016%2fj.vetpar.2013.10.011&partnerID=40&md5=f9944a453e454ecfa3c4431daeb37632}, doi = {10.1016/j.vetpar.2013.10.011}, issn = {03044017}, year = {2014}, date = {2014-01-01}, journal = {Veterinary Parasitology}, volume = {199}, number = {3-4}, pages = {136--143}, abstract = {Enteromyxum leei (Myxozoa) has been identified as the aetiological agent of morbidity and mortality in captive feral parrotfish, Sparisoma cretense held at the facilities of Cretaquarium, the public aquarium of the Hellenic Centre for Marine Research in Crete. The parasite caused substantial mortality to the parrotfish exceeding 60% over a period of 3.5 months. Here we describe the course of disease, the effects of the parasite on the host based on histopathological observations and we provide morphological and molecular data on the parasite. © 2013 Elsevier B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Enteromyxum leei (Myxozoa) has been identified as the aetiological agent of morbidity and mortality in captive feral parrotfish, Sparisoma cretense held at the facilities of Cretaquarium, the public aquarium of the Hellenic Centre for Marine Research in Crete. The parasite caused substantial mortality to the parrotfish exceeding 60% over a period of 3.5 months. Here we describe the course of disease, the effects of the parasite on the host based on histopathological observations and we provide morphological and molecular data on the parasite. © 2013 Elsevier B.V. |
Tsalafouta, A; Papandroulakis, N; Gorissen, M; Katharios, P; Flik, G; Pavlidis, M Ontogenesis of the HPI axis and molecular regulation of the cortisol stress response during early development in Dicentrarchus labrax Journal Article Scientific Reports, 4 , 2014, ISSN: 20452322, (Publisher: Nature Publishing Group). @article{tsalafouta_ontogenesis_2014, title = {Ontogenesis of the HPI axis and molecular regulation of the cortisol stress response during early development in Dicentrarchus labrax}, author = {A Tsalafouta and N Papandroulakis and M Gorissen and P Katharios and G Flik and M Pavlidis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84903781314&doi=10.1038%2fsrep05525&partnerID=40&md5=3f6318c0b65058abe75a45c284c78174}, doi = {10.1038/srep05525}, issn = {20452322}, year = {2014}, date = {2014-01-01}, journal = {Scientific Reports}, volume = {4}, abstract = {The cortisol stress response and the molecular programming of the corticoid axis were characterized for the first time during early ontogeny in a Mediterranean marine teleost, the European sea bass (Dicentrarchus labrax). Sea bass embryos, pre-larvae and larvae at specific points of development were exposed to acute stressors and the temporal patterns of cortisol whole body concentrations and the expression of genes involved in corticosteroid biosynthesis, degradation and signaling were determined. Expression of genes (gr1, gr2, mr, crf) involved into the corticoid response regulation combined with histological data indicated that, although a cortisol stress response is evident for the first time around first feeding, a pattern becomes established in larvae at flexion until the formation of all fins. Moreover, mRNA transcript levels of 11β 2-hydroxylase and 11β 2-hsd2 showed a strong correlation with the whole body cortisol concentrations. Concluding, our data reveal the presence of an adaptive mechanism in European sea bass at early ontogeny enabling to cope with external stressful stimuli and provide a better insight into the onset and regulation of the stress response in this species.}, note = {Publisher: Nature Publishing Group}, keywords = {}, pubstate = {published}, tppubtype = {article} } The cortisol stress response and the molecular programming of the corticoid axis were characterized for the first time during early ontogeny in a Mediterranean marine teleost, the European sea bass (Dicentrarchus labrax). Sea bass embryos, pre-larvae and larvae at specific points of development were exposed to acute stressors and the temporal patterns of cortisol whole body concentrations and the expression of genes involved in corticosteroid biosynthesis, degradation and signaling were determined. Expression of genes (gr1, gr2, mr, crf) involved into the corticoid response regulation combined with histological data indicated that, although a cortisol stress response is evident for the first time around first feeding, a pattern becomes established in larvae at flexion until the formation of all fins. Moreover, mRNA transcript levels of 11β 2-hydroxylase and 11β 2-hsd2 showed a strong correlation with the whole body cortisol concentrations. Concluding, our data reveal the presence of an adaptive mechanism in European sea bass at early ontogeny enabling to cope with external stressful stimuli and provide a better insight into the onset and regulation of the stress response in this species. |
Loizides, M; Georgakopoulou, E; Christou, M; Iliopoulou, M; Papadakis, I; Katharios, P; Divanach, P; Koumoundouros, G Thermally-induced phenotypic plasticity in gilthead seabream Sparus aurata L. (Perciformes, Sparidae) Journal Article Aquaculture, 432 , pp. 383–388, 2014, ISSN: 00448486, (Publisher: Elsevier). @article{loizides_thermally-induced_2014, title = {Thermally-induced phenotypic plasticity in gilthead seabream Sparus aurata L. (Perciformes, Sparidae)}, author = {M Loizides and E Georgakopoulou and M Christou and M Iliopoulou and I Papadakis and P Katharios and P Divanach and G Koumoundouros}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84907594566&doi=10.1016%2fj.aquaculture.2014.06.002&partnerID=40&md5=2d0fb32cff829342c38f272f14f01d7d}, doi = {10.1016/j.aquaculture.2014.06.002}, issn = {00448486}, year = {2014}, date = {2014-01-01}, journal = {Aquaculture}, volume = {432}, pages = {383--388}, abstract = {Finfish present a great ability to modify their phenotype in response to environmental conditions especially in the early ontogenetic period. Such plastic phenotypic responses have a great importance, from both the ecological and aquaculture point of view. The present study examined the effect of water temperature on the ontogeny and juvenile phenotype of Sparus aurata L. Six combinations of three temperatures (16, 19, and 22. °C) were applied during different phases of ontogeny up to the juvenile stage (ca 33-37. mm standard length, SL). Elevated temperature resulted in a relative acceleration of differentiation versus growth, which morphologically was evident as a significantly smaller SL (ca 11% decrease) at different ontogenetic events (development of fins, squamation and body shape). Moreover, geometric morphometric analysis clearly showed a significant effect of water temperature during early ontogeny on the juvenile body-shape. Thermally-induced variation of juvenile body shape was mainly expressed across the first canonical axis (56.4% explained variance), which discriminated the groups of highest temperatures from the rest. Water temperature mainly affected the position of the bases of supraoccipital and of basioccipital bones, of the upper jaw, as well as of the dorsal, anal and pelvic fins. The results are discussed with respect to the potential use of thermal manipulations during the hatchery phase to control the phenotype of S. aurata at the end of on-growing phase, i.e. at commercial size. © 2014 Elsevier B.V.}, note = {Publisher: Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {article} } Finfish present a great ability to modify their phenotype in response to environmental conditions especially in the early ontogenetic period. Such plastic phenotypic responses have a great importance, from both the ecological and aquaculture point of view. The present study examined the effect of water temperature on the ontogeny and juvenile phenotype of Sparus aurata L. Six combinations of three temperatures (16, 19, and 22. °C) were applied during different phases of ontogeny up to the juvenile stage (ca 33-37. mm standard length, SL). Elevated temperature resulted in a relative acceleration of differentiation versus growth, which morphologically was evident as a significantly smaller SL (ca 11% decrease) at different ontogenetic events (development of fins, squamation and body shape). Moreover, geometric morphometric analysis clearly showed a significant effect of water temperature during early ontogeny on the juvenile body-shape. Thermally-induced variation of juvenile body shape was mainly expressed across the first canonical axis (56.4% explained variance), which discriminated the groups of highest temperatures from the rest. Water temperature mainly affected the position of the bases of supraoccipital and of basioccipital bones, of the upper jaw, as well as of the dorsal, anal and pelvic fins. The results are discussed with respect to the potential use of thermal manipulations during the hatchery phase to control the phenotype of S. aurata at the end of on-growing phase, i.e. at commercial size. © 2014 Elsevier B.V. |
2013 |
NE., Ioannidis; A., Papazi; G., Tsoukali; T., Tsiavos; P., Katharios; P., Divanach; Kotzabasis, K The physiology of Chlorella minutissima cultures in a modern low cost photobioreactor designed for high productivity. Inproceedings HBS, (Ed.): Thessaloniki, Greece, 2013. @inproceedings{ioannidis_physiology_2013, title = {The physiology of Chlorella minutissima cultures in a modern low cost photobioreactor designed for high productivity.}, author = {Ioannidis NE. and Papazi A. and Tsoukali G. and Tsiavos T. and Katharios P. and Divanach P. and K Kotzabasis}, editor = {HBS}, year = {2013}, date = {2013-10-01}, address = {Thessaloniki, Greece}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
V., Kostopoulou; A., Tsopelakos; E., Zogopoulou; H., Miliou; P., Divanach; Katharios, P The effect of different phytoplankton species and commercial enrichment products on the fatty acid profile, enzyme activity and overall condition of the rotifer Brachionus plicatilis. Inproceedings of Ghent, University (Ed.): Larve 2013, pp. 219–222, Ghent, Belgium, 2013. @inproceedings{kostopoulou_effect_2013-1, title = {The effect of different phytoplankton species and commercial enrichment products on the fatty acid profile, enzyme activity and overall condition of the rotifer Brachionus plicatilis.}, author = {Kostopoulou V. and Tsopelakos A. and Zogopoulou E. and Miliou H. and Divanach P. and P Katharios}, editor = {University of Ghent}, year = {2013}, date = {2013-09-01}, booktitle = {Larve 2013}, pages = {219--222}, address = {Ghent, Belgium}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Katharios, P Pathological problems of meagre Journal Article Fishing News, 379 , pp. 24–37, 2013. @article{katharios_pathological_2013, title = {Pathological problems of meagre}, author = {P Katharios}, year = {2013}, date = {2013-08-01}, journal = {Fishing News}, volume = {379}, pages = {24--37}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Rigos, G; Karagouni, E; Kyriazis, I; Athanasiou, E; Grigorakis, K; Kotou, E; Katharios, P In vitro and in vivo evaluation of quinine in gilthead sea bream, Sparus aurata naturally infected with the ciliate Cryptocaryon irritans Journal Article Aquaculture, 416-417 , pp. 185–191, 2013, ISSN: 00448486. @article{rigos_vitro_2013, title = {In vitro and in vivo evaluation of quinine in gilthead sea bream, Sparus aurata naturally infected with the ciliate Cryptocaryon irritans}, author = {G Rigos and E Karagouni and I Kyriazis and E Athanasiou and K Grigorakis and E Kotou and P Katharios}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84884939051&doi=10.1016%2fj.aquaculture.2013.09.021&partnerID=40&md5=0f7732d82be918191ceed6ca9f8115d8}, doi = {10.1016/j.aquaculture.2013.09.021}, issn = {00448486}, year = {2013}, date = {2013-01-01}, journal = {Aquaculture}, volume = {416-417}, pages = {185--191}, abstract = {The use of quinine as a potential antiparasitic compound was evaluated at cellular level and in naturally infected gilthead sea bream, Sparus aurata with the ciliate Cryptocaryon irritans. Quinine concentrations up to 10. μg/ml (30. μΜ) did not exhibit any in vitro hemolytic or pro-apoptotic effects on red blood cells or head kidney macrophages, respectively. Dietary administration of quinine in gilthead sea bream at a concentration of 100. mg/kg fish for 10. days was incomplete due to palatability problems after the fifth day post treatment. A decreasing distribution profile of quinine was evident in the analyzed tissues even during the period where quinine consumption was supposedly ensured. The highest concentrations were measured on day 1 reaching 8.98. ±. 3.85. μg/ml (27. μΜ), 1.33. ±. 0.45. μg/g and 0.75. ±. 0.48. μg/g in plasma, skin and gills respectively. Quinine was rapidly removed from the tissue compartment while it remained at low concentration in fish circulation after therapy. A 100% C. irritans prevalence was accompanied with no mortalities during the experiment. Parasitic intensity, measured as trophont number, was significantly reduced in quinine-treated fish at the first sampling point (3rd day: 8. ±. 0.8 vs 11. ±. 2.6), while no statistical differences were observed thereafter. A significant reduction in plasma glucose level was also observed at the same time point. Organoleptic evaluation in fillets, showed no difference in the flesh bitterness between quinine-treated and control fish.In conclusion, quinine has no cytotoxic effect on the assessed fish cells and is adequately absorbed in circulation, affecting the glycemic level but its distribution to the targeted tissues by the ciliate tissues is relatively small. Quinine significantly reduced C. irritans' intensity in lightly-infected gilthead sea bream. The sensory attributes of quinine-treated fish are not affected by the treatment. © 2013 Elsevier B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The use of quinine as a potential antiparasitic compound was evaluated at cellular level and in naturally infected gilthead sea bream, Sparus aurata with the ciliate Cryptocaryon irritans. Quinine concentrations up to 10. μg/ml (30. μΜ) did not exhibit any in vitro hemolytic or pro-apoptotic effects on red blood cells or head kidney macrophages, respectively. Dietary administration of quinine in gilthead sea bream at a concentration of 100. mg/kg fish for 10. days was incomplete due to palatability problems after the fifth day post treatment. A decreasing distribution profile of quinine was evident in the analyzed tissues even during the period where quinine consumption was supposedly ensured. The highest concentrations were measured on day 1 reaching 8.98. ±. 3.85. μg/ml (27. μΜ), 1.33. ±. 0.45. μg/g and 0.75. ±. 0.48. μg/g in plasma, skin and gills respectively. Quinine was rapidly removed from the tissue compartment while it remained at low concentration in fish circulation after therapy. A 100% C. irritans prevalence was accompanied with no mortalities during the experiment. Parasitic intensity, measured as trophont number, was significantly reduced in quinine-treated fish at the first sampling point (3rd day: 8. ±. 0.8 vs 11. ±. 2.6), while no statistical differences were observed thereafter. A significant reduction in plasma glucose level was also observed at the same time point. Organoleptic evaluation in fillets, showed no difference in the flesh bitterness between quinine-treated and control fish.In conclusion, quinine has no cytotoxic effect on the assessed fish cells and is adequately absorbed in circulation, affecting the glycemic level but its distribution to the targeted tissues by the ciliate tissues is relatively small. Quinine significantly reduced C. irritans' intensity in lightly-infected gilthead sea bream. The sensory attributes of quinine-treated fish are not affected by the treatment. © 2013 Elsevier B.V. |
Georgakopoulou, E; Loizides, M; Christou, M; Iliopoulou, M; Papadakis, I; Katharios, P; Divanach, P; Koumoundouros, G Thermally-induced phenotypic plasticity in gilthead sea bream. Journal Article Communications in agricultural and applied biological sciences, 78 (4), pp. 143–146, 2013, ISSN: 13791176. @article{georgakopoulou_thermally-induced_2013, title = {Thermally-induced phenotypic plasticity in gilthead sea bream.}, author = {E Georgakopoulou and M Loizides and M Christou and M Iliopoulou and I Papadakis and P Katharios and P Divanach and G Koumoundouros}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84907271903&partnerID=40&md5=fc2e59b41c153fa8c094c3eb304c3191}, issn = {13791176}, year = {2013}, date = {2013-01-01}, journal = {Communications in agricultural and applied biological sciences}, volume = {78}, number = {4}, pages = {143--146}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Kostopoulou, V; Tsopelakos, A; Zogopoulou, E; Miliou, H; Divanach, P; Katharios, P Communications in agricultural and applied biological sciences, 78 (4), pp. 219–222, 2013, ISSN: 13791176. @article{kostopoulou_effect_2013, title = {The effect of different phytoplankton species and commercial enrichment products on the fatty acid profile, enzyme activity, and overall condition of the rotifer Brachionus Plicatilis.}, author = {V Kostopoulou and A Tsopelakos and E Zogopoulou and H Miliou and P Divanach and P Katharios}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84907278292&partnerID=40&md5=a21b495ca8214710c394fe4eff5e1fba}, issn = {13791176}, year = {2013}, date = {2013-01-01}, journal = {Communications in agricultural and applied biological sciences}, volume = {78}, number = {4}, pages = {219--222}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Kalatzis, P G; Kokkari, C; Katharios, P Parasitology Research, 112 (5), pp. 2055–2061, 2013, ISSN: 09320113. @article{kalatzis_description_2013, title = {Description and relationships of two novel species of Ceratomyxa Thelohan, 1892 infecting the gallbladders of Aulopiformes: Atlantic lizardfish Synodus saurus Linnaeus, 1758 and royal flagfin Aulopus filamentosus Bloch, 1792 from Cretan Sea, Greece}, author = {P G Kalatzis and C Kokkari and P Katharios}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84876289084&doi=10.1007%2fs00436-013-3366-7&partnerID=40&md5=a06d035fd72a62aa863bce67aeed3dbb}, doi = {10.1007/s00436-013-3366-7}, issn = {09320113}, year = {2013}, date = {2013-01-01}, journal = {Parasitology Research}, volume = {112}, number = {5}, pages = {2055--2061}, abstract = {Two members of Aulopiformes (grinners) were examined for myxosporean parasites in their gallbladders. Synodus saurus and Aulopus filamentosus from Cretan Sea, Greece were infected by Ceratomyxa parasites which are described in this paper. Both species of Ceratomyxa are new and referred for the first time as Ceratomyxa cretensis n.sp. and Ceratomyxa filamentosi n.sp. The new species are described morphologically by light and scanning electron microscopy analysis, and characterized genetically by sequencing the small subunit (SSU) rDNA. The SSU rDNA sequences obtained from the two novel Ceratomyxa species were used for the construction of a phylogenetic tree with all the available Ceratomyxa SSU rDNA sequences. © 2013 Springer-Verlag Berlin Heidelberg.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Two members of Aulopiformes (grinners) were examined for myxosporean parasites in their gallbladders. Synodus saurus and Aulopus filamentosus from Cretan Sea, Greece were infected by Ceratomyxa parasites which are described in this paper. Both species of Ceratomyxa are new and referred for the first time as Ceratomyxa cretensis n.sp. and Ceratomyxa filamentosi n.sp. The new species are described morphologically by light and scanning electron microscopy analysis, and characterized genetically by sequencing the small subunit (SSU) rDNA. The SSU rDNA sequences obtained from the two novel Ceratomyxa species were used for the construction of a phylogenetic tree with all the available Ceratomyxa SSU rDNA sequences. © 2013 Springer-Verlag Berlin Heidelberg. |
Sfakianakis, D G; Katharios, P; Tsirigotakis, N; Doxa, C K; Kentouri, M Lateral line deformities in wild and farmed sea bass (Dicentrarchus labrax, L.) and sea bream (Sparus aurata, L.) Journal Article Journal of Applied Ichthyology, 29 (5), pp. 1015–1021, 2013, ISSN: 01758659. @article{sfakianakis_lateral_2013, title = {Lateral line deformities in wild and farmed sea bass (Dicentrarchus labrax, L.) and sea bream (Sparus aurata, L.)}, author = {D G Sfakianakis and P Katharios and N Tsirigotakis and C K Doxa and M Kentouri}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84883744109&doi=10.1111%2fjai.12248&partnerID=40&md5=4c1685a4a6dae5a7dcda84385a0c14cc}, doi = {10.1111/jai.12248}, issn = {01758659}, year = {2013}, date = {2013-01-01}, journal = {Journal of Applied Ichthyology}, volume = {29}, number = {5}, pages = {1015--1021}, abstract = {Summary: The lateral line of aquaculture fishes has rarely been studied although it is a very important anatomical organ that could serve as an inexpensive and easy tool to distinguish farmed from wild individuals. In the present study, lateral line deformities were examined in both wild and farmed sea bass (Dicentrarchus labrax) and sea bream (Sparus aurata) specimens to try to detail all possible differences between them. In order to do so, the morphology of the trunk lateral line in wild and farmed adults was examined whereby two major deformities were observed in both species: the 'scale pocket' deformity (14-40% incidence in all groups) where the specialized scales are missing but the canal underneath is present and the scale print is obvious, and the 'somatic scales' deformity (14-56% incidence in farmed individuals only) where the missing lateral line is covered with normal somatic scales. Histological examination confirmed the macroscopic observations in that the lateral line mechanism was present - although damaged - beneath the scale pocket deformity and completely absent beneath the somatic scales deformity. It is argued that the scale pocket deformity is a result of an accident during the life of the fish whereas the somatic scales deformity is an actual deformity in development. © 2013 Blackwell Verlag GmbH.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Summary: The lateral line of aquaculture fishes has rarely been studied although it is a very important anatomical organ that could serve as an inexpensive and easy tool to distinguish farmed from wild individuals. In the present study, lateral line deformities were examined in both wild and farmed sea bass (Dicentrarchus labrax) and sea bream (Sparus aurata) specimens to try to detail all possible differences between them. In order to do so, the morphology of the trunk lateral line in wild and farmed adults was examined whereby two major deformities were observed in both species: the 'scale pocket' deformity (14-40% incidence in all groups) where the specialized scales are missing but the canal underneath is present and the scale print is obvious, and the 'somatic scales' deformity (14-56% incidence in farmed individuals only) where the missing lateral line is covered with normal somatic scales. Histological examination confirmed the macroscopic observations in that the lateral line mechanism was present - although damaged - beneath the scale pocket deformity and completely absent beneath the somatic scales deformity. It is argued that the scale pocket deformity is a result of an accident during the life of the fish whereas the somatic scales deformity is an actual deformity in development. © 2013 Blackwell Verlag GmbH. |
2011 |
R., Bastias; G., Higuera; D., Castillo; R., Espejo; M., Tediashvili; M., Kutateladze; E., Tevdoradze; T., Kokashvili; Katharios, P Phage isolation and phage therapy against fish bacterial pathogens. Inproceedings EAS, (Ed.): Aquaculture Europe 11, pp. 96–97, Rhodes, Greece, 2011. @inproceedings{bastias_phage_2011, title = {Phage isolation and phage therapy against fish bacterial pathogens.}, author = {Bastias R. and Higuera G. and Castillo D. and Espejo R. and Tediashvili M. and Kutateladze M. and Tevdoradze E. and Kokashvili T. and P Katharios}, editor = {EAS}, year = {2011}, date = {2011-10-01}, booktitle = {Aquaculture Europe 11}, pages = {96--97}, address = {Rhodes, Greece}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Katharios, P; Kokkari, K; Papadaki, M; Papandroulakis, N Systemic granulomas in cultured meagre, Argyrosomus regius. Inproceedings pp. 537–538, European Aquaculture Society, Rhodes, Greece, 2011, (Publication Title: Aquaculture Europe 11 Type: Conference Paper). @inproceedings{katharios_systemic_2011, title = {Systemic granulomas in cultured meagre, Argyrosomus regius.}, author = {P Katharios and K Kokkari and M Papadaki and N Papandroulakis}, year = {2011}, date = {2011-10-01}, pages = {537--538}, publisher = {European Aquaculture Society}, address = {Rhodes, Greece}, note = {Publication Title: Aquaculture Europe 11 Type: Conference Paper}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Katharios, P; Rigos, G; Divanach, P Enteromyxum leei (Myxozoa), a Lethal Intruder of Tropical Pet Fish: First Case in Humphead Wrasse, Cheilinus undulatus (Rüppell, 1835) Journal Article Journal of Exotic Pet Medicine, 20 (2), pp. 138–143, 2011, ISSN: 15575063. @article{katharios_enteromyxum_2011, title = {Enteromyxum leei (Myxozoa), a Lethal Intruder of Tropical Pet Fish: First Case in Humphead Wrasse, Cheilinus undulatus (Rüppell, 1835)}, author = {P Katharios and G Rigos and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79955634789&doi=10.1053%2fj.jepm.2011.02.009&partnerID=40&md5=e3c00ff0668cec7f9df0c2a21f5fd013}, doi = {10.1053/j.jepm.2011.02.009}, issn = {15575063}, year = {2011}, date = {2011-01-01}, journal = {Journal of Exotic Pet Medicine}, volume = {20}, number = {2}, pages = {138--143}, abstract = {The lethal effect of enteromyxosis is recorded for the first time in the humphead wrasse, Cheilinus undulatus, a tropical pet fish. Enteromyxosis was caused by the myxosporean parasite Enteromyxum leei, and the gut was the only tissue affected in this case. Severe enteritis and degeneration of the intestinal mucosa may have caused a digestive function disorder, thereby resulting in the fish's death. The histopathological findings of this case included destruction of normal tissue architecture of the intestinal mucosa, chronic inflammation, and infiltration of mast cells/eosinophilic granular cells, which resembled findings described in other fish that were diagnosed with enteromyxosis. Induced stress by transportation and entrance to a new environment possibly played an underlying role in the virulence of the parasitic infection and associated tissue pathology. © 2011 Elsevier Inc.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The lethal effect of enteromyxosis is recorded for the first time in the humphead wrasse, Cheilinus undulatus, a tropical pet fish. Enteromyxosis was caused by the myxosporean parasite Enteromyxum leei, and the gut was the only tissue affected in this case. Severe enteritis and degeneration of the intestinal mucosa may have caused a digestive function disorder, thereby resulting in the fish's death. The histopathological findings of this case included destruction of normal tissue architecture of the intestinal mucosa, chronic inflammation, and infiltration of mast cells/eosinophilic granular cells, which resembled findings described in other fish that were diagnosed with enteromyxosis. Induced stress by transportation and entrance to a new environment possibly played an underlying role in the virulence of the parasitic infection and associated tissue pathology. © 2011 Elsevier Inc. |
Katharios, P; Papadaki, M; Ternengo, S; Kantham, P K; Zeri, C; Petraki, P E; Divanach, P Chronic ulcerative dermatopathy in cultured marine fishes. Comparative study in sharpsnout sea bream, Diplodus puntazzo (Walbaum) Journal Article Journal of Fish Diseases, 34 (6), pp. 459–474, 2011, ISSN: 01407775. @article{katharios_chronic_2011, title = {Chronic ulcerative dermatopathy in cultured marine fishes. Comparative study in sharpsnout sea bream, Diplodus puntazzo (Walbaum)}, author = {P Katharios and M Papadaki and S Ternengo and P K Kantham and C Zeri and P E Petraki and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79955640274&doi=10.1111%2fj.1365-2761.2011.01257.x&partnerID=40&md5=da9a133e20c28b1376b9ba550b57d497}, doi = {10.1111/j.1365-2761.2011.01257.x}, issn = {01407775}, year = {2011}, date = {2011-01-01}, journal = {Journal of Fish Diseases}, volume = {34}, number = {6}, pages = {459--474}, abstract = {Chronic ulcerative dermatopathy (CUD) also known as chronic erosive dermatopathy, hole-in-the-head, head and lateral line erosion syndrome (HLLE) and lateral line depigmentation (LLD) is a chronic disease of unknown aetiology that affects the lateral line canals of the head and the trunk of various fish species. It has been described only in freshwater species although there are reports that it also affects marine fish. Here, we describe the disease in cultured sharpsnout sea bream using histology and scanning electron microscopy and identify several marine species as CUD sensitive. The results of this study correlate the development of the disease with the use of borehole water, indicating that the aetiology is probably associated with water quality rather than nutritional imbalance or infectious agents. © 2011 Blackwell Publishing Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Chronic ulcerative dermatopathy (CUD) also known as chronic erosive dermatopathy, hole-in-the-head, head and lateral line erosion syndrome (HLLE) and lateral line depigmentation (LLD) is a chronic disease of unknown aetiology that affects the lateral line canals of the head and the trunk of various fish species. It has been described only in freshwater species although there are reports that it also affects marine fish. Here, we describe the disease in cultured sharpsnout sea bream using histology and scanning electron microscopy and identify several marine species as CUD sensitive. The results of this study correlate the development of the disease with the use of borehole water, indicating that the aetiology is probably associated with water quality rather than nutritional imbalance or infectious agents. © 2011 Blackwell Publishing Ltd. |
P., Kokkinias; I., Georga; I.E., Papadakis; P., Katharios; C., Batargias; P., Divanach; G., Koumoundouros Effect of early temperature on the osteological morphometry of European sea bass juveniles. Inproceedings EAS, (Ed.): pp. 564–565, Rhodes, Greece, 2011. @inproceedings{kokkinias_effect_2011, title = {Effect of early temperature on the osteological morphometry of European sea bass juveniles.}, author = {Kokkinias P. and Georga I. and Papadakis I.E. and Katharios P. and Batargias C. and Divanach P. and Koumoundouros G.}, editor = {EAS}, year = {2011}, date = {2011-01-01}, pages = {564--565}, address = {Rhodes, Greece}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
2010 |
Katharios, P Infectious fish diseases in Mediterranean mariculture Journal Article Fish Farming Expert, (5), pp. 33–45, 2010, (Place: Bergn, Norway). @article{katharios_infectious_2010, title = {Infectious fish diseases in Mediterranean mariculture}, author = {P Katharios}, year = {2010}, date = {2010-11-01}, journal = {Fish Farming Expert}, number = {5}, pages = {33--45}, note = {Place: Bergn, Norway}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Katharios, P Experimental study on Head and Lateral Line Erosion Syndrome Inproceedings EUAC, (Ed.): 27th EUAC Meeting, Cretaquarium, Heraklion, Greece, 2010. @inproceedings{katharios_experimental_2010, title = {Experimental study on Head and Lateral Line Erosion Syndrome}, author = {P Katharios}, editor = {EUAC}, year = {2010}, date = {2010-10-01}, booktitle = {27th EUAC Meeting}, address = {Cretaquarium, Heraklion, Greece}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Rigos, G; Katharios, P Pathological obstacles of newly-introduced fish species in Mediterranean mariculture: A review Journal Article Reviews in Fish Biology and Fisheries, 20 (1), pp. 47–70, 2010, ISSN: 09603166. @article{rigos_pathological_2010, title = {Pathological obstacles of newly-introduced fish species in Mediterranean mariculture: A review}, author = {G Rigos and P Katharios}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-76649096331&doi=10.1007%2fs11160-009-9120-7&partnerID=40&md5=47199e5ef09af01c2cd4e97aa9274262}, doi = {10.1007/s11160-009-9120-7}, issn = {09603166}, year = {2010}, date = {2010-01-01}, journal = {Reviews in Fish Biology and Fisheries}, volume = {20}, number = {1}, pages = {47--70}, abstract = {Species diversification has been extensively employed in Mediterranean mariculture industry as a tool to relief the crisis resulting from market saturation and overproduction of the two main representatives, sea bass (Dicentrarchus labrax) and gilthead sea bream (Sparus aurata). Numerous new promising candidates belonging to several fish families such as Sparidae, Polyprionidae, Serranidae, Sciaenidae, Soleidae, Carangidae and Scombridae have been introduced to provide alternative culture choices in Mediterranean. The farming potential of these species in production scale has received some or little success partly due to the fact that their entrance has not been accompanied without the presence of considerable pathological problems. Bacterial outbreaks causing serious problems have been mainly induced by Photobacterium damsella subsp. piscicida, Vibrio alginolyticus, V. harveyi, Tenacibaculum maritimum and Flavobacterium sp. Detrimental parasitic pathogens included the myxosporean Enteromyxum leei, the ciliates Cryptocaryon irritans and Uronema spp., the flagellate Amyloodinium occelatum, the monogenean Zeuxapta seriolae and the digeneans Paradeontacylix-like spp. Viral encephalopathy and retinopathy infections and the chlamydia-like bacteria causing Epitheliocystis disease are also of significance importance. Numerous other agents of lesser pathogenic significance as well as other occasionally recorded pathogens are also mentioned in this review. Additional research effort has to be devoted to the pathogenesis of important pathogens and the interactions with their hosts to assist the viability of new fish species farming in Mediterranean region. © Springer Science+Business Media B.V. 2009.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Species diversification has been extensively employed in Mediterranean mariculture industry as a tool to relief the crisis resulting from market saturation and overproduction of the two main representatives, sea bass (Dicentrarchus labrax) and gilthead sea bream (Sparus aurata). Numerous new promising candidates belonging to several fish families such as Sparidae, Polyprionidae, Serranidae, Sciaenidae, Soleidae, Carangidae and Scombridae have been introduced to provide alternative culture choices in Mediterranean. The farming potential of these species in production scale has received some or little success partly due to the fact that their entrance has not been accompanied without the presence of considerable pathological problems. Bacterial outbreaks causing serious problems have been mainly induced by Photobacterium damsella subsp. piscicida, Vibrio alginolyticus, V. harveyi, Tenacibaculum maritimum and Flavobacterium sp. Detrimental parasitic pathogens included the myxosporean Enteromyxum leei, the ciliates Cryptocaryon irritans and Uronema spp., the flagellate Amyloodinium occelatum, the monogenean Zeuxapta seriolae and the digeneans Paradeontacylix-like spp. Viral encephalopathy and retinopathy infections and the chlamydia-like bacteria causing Epitheliocystis disease are also of significance importance. Numerous other agents of lesser pathogenic significance as well as other occasionally recorded pathogens are also mentioned in this review. Additional research effort has to be devoted to the pathogenesis of important pathogens and the interactions with their hosts to assist the viability of new fish species farming in Mediterranean region. © Springer Science+Business Media B.V. 2009. |
Georgakopoulou, E; Katharios, P; Divanach, P; Koumoundouros, G Effect of temperature on the development of skeletal deformities in Gilthead seabream (Sparus aurata Linnaeus, 1758) Journal Article Aquaculture, 308 (1-2), pp. 13–19, 2010, ISSN: 00448486. @article{georgakopoulou_effect_2010, title = {Effect of temperature on the development of skeletal deformities in Gilthead seabream (Sparus aurata Linnaeus, 1758)}, author = {E Georgakopoulou and P Katharios and P Divanach and G Koumoundouros}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77956618251&doi=10.1016%2fj.aquaculture.2010.08.006&partnerID=40&md5=0385694485812464aad46479cf10e535}, doi = {10.1016/j.aquaculture.2010.08.006}, issn = {00448486}, year = {2010}, date = {2010-01-01}, journal = {Aquaculture}, volume = {308}, number = {1-2}, pages = {13--19}, abstract = {The development of skeletal deformities is an important problem for finfish hatcheries. In the present paper, the effect of water temperature on the development of skeletal deformities in Sparus aurata was examined. Six combinations of three temperatures (16, 19, and 22°C) were applied during three ontogenetic windows: the autotrophic (embryonic and yolk-sac larval stages), the exotrophic larval (first feeding to metamorphosing larvae of 14-16. mm TL) and the juvenile (metamorphosing larvae of 14-16. mm TL to juveniles of 40-45 mm TL) periods. The results demonstrated a significant effect (ptextless 0.05) of water temperature on the development of inside folded gill-cover, haemal lordosis, as well as of mild deformities of the caudal and dorsal fin-supporting elements. The prevalence of gill-cover deformities was elevated when 16°C water temperature was applied during the autotrophic and exotrophic larval periods (50.0 ± 2.8%, mean ± SD), or only during the autotrophic period (14.0 ± 0.0%). Haemal lordosis development, the second most severe skeletal deformity, presented fluctuating response against water temperature up to 14-16. mm TL (3.0 ± 4.2 to 13.0 ± 9.9%). However, the application of 22°C during the juvenile period resulted in the lowest and less variable incidence of haemal lordosis (1.0 ± 0.0 to 5.0 ± 1.4%). The mild deformities of caudal and dorsal fins presented different responses to water temperature. The prevalence of caudal-fin deformities was elevated when 16°C temperature was applied during the exotrophic larval period (54.0 ± 8.5%), while dorsal-fin deformities were favoured when 22°C temperature was applied during the autotrophic and exotrophic phases (35.0 ± 9.9 to 39.0 ± 4.2%). In the examined thermal range, growth rate was significantly elevated with the temperature increase. Fish survival was higher in the treatments where temperature after yolk-consumption increased from 16 or 19°C to 19 or 22°C, respectively. The results are discussed in respect to the onset of ontogeny of the different skeletal elements and the need of applying different thermal conditions during the development of S. aurata. © 2010 Elsevier B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The development of skeletal deformities is an important problem for finfish hatcheries. In the present paper, the effect of water temperature on the development of skeletal deformities in Sparus aurata was examined. Six combinations of three temperatures (16, 19, and 22°C) were applied during three ontogenetic windows: the autotrophic (embryonic and yolk-sac larval stages), the exotrophic larval (first feeding to metamorphosing larvae of 14-16. mm TL) and the juvenile (metamorphosing larvae of 14-16. mm TL to juveniles of 40-45 mm TL) periods. The results demonstrated a significant effect (ptextless 0.05) of water temperature on the development of inside folded gill-cover, haemal lordosis, as well as of mild deformities of the caudal and dorsal fin-supporting elements. The prevalence of gill-cover deformities was elevated when 16°C water temperature was applied during the autotrophic and exotrophic larval periods (50.0 ± 2.8%, mean ± SD), or only during the autotrophic period (14.0 ± 0.0%). Haemal lordosis development, the second most severe skeletal deformity, presented fluctuating response against water temperature up to 14-16. mm TL (3.0 ± 4.2 to 13.0 ± 9.9%). However, the application of 22°C during the juvenile period resulted in the lowest and less variable incidence of haemal lordosis (1.0 ± 0.0 to 5.0 ± 1.4%). The mild deformities of caudal and dorsal fins presented different responses to water temperature. The prevalence of caudal-fin deformities was elevated when 16°C temperature was applied during the exotrophic larval period (54.0 ± 8.5%), while dorsal-fin deformities were favoured when 22°C temperature was applied during the autotrophic and exotrophic phases (35.0 ± 9.9 to 39.0 ± 4.2%). In the examined thermal range, growth rate was significantly elevated with the temperature increase. Fish survival was higher in the treatments where temperature after yolk-consumption increased from 16 or 19°C to 19 or 22°C, respectively. The results are discussed in respect to the onset of ontogeny of the different skeletal elements and the need of applying different thermal conditions during the development of S. aurata. © 2010 Elsevier B.V. |
Katharios, P; Tsigenopoulos, C S First report of nodavirus outbreak in cultured juvenile shi drum, Umbrina cirrosa L., in Greece Journal Article Aquaculture Research, 42 , pp. 147–152, 2010, ISSN: 1355557X. @article{katharios_first_2010, title = {First report of nodavirus outbreak in cultured juvenile shi drum, Umbrina cirrosa L., in Greece}, author = {P Katharios and C S Tsigenopoulos}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-78650132503&doi=10.1111%2fj.1365-2109.2010.02532.x&partnerID=40&md5=9fb2484d5ca280c83fd3574d9a935e71}, doi = {10.1111/j.1365-2109.2010.02532.x}, issn = {1355557X}, year = {2010}, date = {2010-01-01}, journal = {Aquaculture Research}, volume = {42}, pages = {147--152}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Rigos, G; Katharios, P; Papandroulakis, N Single intramuscular administration of long-acting oxytetracycline in grouper (Epinephelus marginatus) Journal Article Turkish Journal of Veterinary and Animal Sciences, 34 (5), pp. 441–445, 2010, ISSN: 13000128. @article{rigos_single_2010, title = {Single intramuscular administration of long-acting oxytetracycline in grouper (Epinephelus marginatus)}, author = {G Rigos and P Katharios and N Papandroulakis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-78650669591&doi=10.3906%2fvet-0906-10&partnerID=40&md5=31f361ba713c356eb768142370d17368}, doi = {10.3906/vet-0906-10}, issn = {13000128}, year = {2010}, date = {2010-01-01}, journal = {Turkish Journal of Veterinary and Animal Sciences}, volume = {34}, number = {5}, pages = {441--445}, abstract = {The plasma concentration of long-acting oxytetracycline (OTC-LA) was measured following a single intramuscular (IM) injection (50 mg/kg) in grouper (Epinephelus marginatus), a common inhabitant of marine aquaria and a potential candidate for fish farming. The experiment was carried out at 20 °C and sampling points were selected at 0.25, 0.5, 1, 2, 4, 6, 8, 12, 24, and 48 h post-administration. OTC concentration in grouper circulation was maintained at high levels for the whole experiment (8.35-39.67 μg/mL) with maximum plasma concentration measured at 1 h postinjection. The area under the curve values was also promising since AUC0-24 and AUC 0-48 were calculated to be high (363.45 and 668.73 μg h/mL, respectively). Due to the high OTC levels accomplished for prolonged period of time, a single administration of OTC-LA could be an ideal route in cases when multiple handling is unwanted and big animals such as grouper are to be treated. © Tübi̇tak.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The plasma concentration of long-acting oxytetracycline (OTC-LA) was measured following a single intramuscular (IM) injection (50 mg/kg) in grouper (Epinephelus marginatus), a common inhabitant of marine aquaria and a potential candidate for fish farming. The experiment was carried out at 20 °C and sampling points were selected at 0.25, 0.5, 1, 2, 4, 6, 8, 12, 24, and 48 h post-administration. OTC concentration in grouper circulation was maintained at high levels for the whole experiment (8.35-39.67 μg/mL) with maximum plasma concentration measured at 1 h postinjection. The area under the curve values was also promising since AUC0-24 and AUC 0-48 were calculated to be high (363.45 and 668.73 μg h/mL, respectively). Due to the high OTC levels accomplished for prolonged period of time, a single administration of OTC-LA could be an ideal route in cases when multiple handling is unwanted and big animals such as grouper are to be treated. © Tübi̇tak. |
2009 |
Ternengo, S; Quilichini, Y; Katharios, P; Marchand, B Parasitology Research, 104 (4), pp. 801–807, 2009, ISSN: 09320113. @article{ternengo_sperm_2009, title = {Sperm ultrastructure of the gall bladder fluke Anisocoelium capitellatum (Digenea: Cryptogonimidae), a parasite of Uranoscopus scaber (Pisces: Uranoscopidae)}, author = {S Ternengo and Y Quilichini and P Katharios and B Marchand}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-65549115445&doi=10.1007%2fs00436-008-1259-y&partnerID=40&md5=380759291a15cb1fd00df0fcf025ff2e}, doi = {10.1007/s00436-008-1259-y}, issn = {09320113}, year = {2009}, date = {2009-01-01}, journal = {Parasitology Research}, volume = {104}, number = {4}, pages = {801--807}, abstract = {Ultrastructure of mature spermatozoon of Anisocoelium capitellatum is described with transmission electron microscopy. The description gave evidence of some characteristics of this digenean. The male gamete presents features allowing the distinction between A. capitellatum and other digenetic trematodes. It is characterized by original anterior and posterior extremities. The peripheral doublets appear prior to the central cores in the anterior extremity of axoneme. In the distal part of the spermatozoon, these ultrastructural features are also the first elements disappearing in the axoneme 1 and the last remaining in the axoneme 2. Another important peculiarity of the spermatozoon is the presence of three mitochondria. © 2008 Springer-Verlag.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Ultrastructure of mature spermatozoon of Anisocoelium capitellatum is described with transmission electron microscopy. The description gave evidence of some characteristics of this digenean. The male gamete presents features allowing the distinction between A. capitellatum and other digenetic trematodes. It is characterized by original anterior and posterior extremities. The peripheral doublets appear prior to the central cores in the anterior extremity of axoneme. In the distal part of the spermatozoon, these ultrastructural features are also the first elements disappearing in the axoneme 1 and the last remaining in the axoneme 2. Another important peculiarity of the spermatozoon is the presence of three mitochondria. © 2008 Springer-Verlag. |
Katharios, P; Divanach, P; Georgakopoulou, E; Koumoundouros, G Temperature limits for gilthead seabream and seabass Incollection FEAP, (Ed.): Control of malformation in fish aquaculture: Science and practice, pp. 43–45, Federation of European Aquaculture Producers, Belgium, 2009. @incollection{katharios_temperature_2009, title = {Temperature limits for gilthead seabream and seabass}, author = {P Katharios and P Divanach and E Georgakopoulou and G Koumoundouros}, editor = {FEAP}, year = {2009}, date = {2009-01-01}, booktitle = {Control of malformation in fish aquaculture: Science and practice}, pages = {43--45}, publisher = {Federation of European Aquaculture Producers}, address = {Belgium}, keywords = {}, pubstate = {published}, tppubtype = {incollection} } |
Katharios, P Transition from hatchery to cages. Considerations for proper health management in sea bass and sea bream farming. Journal Article Fish Farming Expert, (4), pp. 43–52, 2009, (Place: Bergen, Norway). @article{katharios_transition_2009, title = {Transition from hatchery to cages. Considerations for proper health management in sea bass and sea bream farming.}, author = {P Katharios}, year = {2009}, date = {2009-01-01}, journal = {Fish Farming Expert}, number = {4}, pages = {43--52}, note = {Place: Bergen, Norway}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Zambonino-Infante, J L; Katharios, P; Koumoundouros, G; Tandler, A Recommendations on prevention o malformations in seabass and seabream Incollection FEAP, (Ed.): Control of malformation in fish aquaculture: Science and practice, pp. 141–145, Federation of European Aquaculture Producers, Belgium, 2009. @incollection{zambonino-infante_recommendations_2009, title = {Recommendations on prevention o malformations in seabass and seabream}, author = {J L Zambonino-Infante and P Katharios and G Koumoundouros and A Tandler}, editor = {FEAP}, year = {2009}, date = {2009-01-01}, booktitle = {Control of malformation in fish aquaculture: Science and practice}, pages = {141--145}, publisher = {Federation of European Aquaculture Producers}, address = {Belgium}, keywords = {}, pubstate = {published}, tppubtype = {incollection} } |
2008 |
Ternengo, S; Katharios, P Microcotylid parasites: an emerging problem in Mediterranean cage aquaculture. Journal Article Fish Farming Expert, pp. 44–48, 2008, (Place: Bergen, Norway). @article{ternengo_microcotylid_2008, title = {Microcotylid parasites: an emerging problem in Mediterranean cage aquaculture.}, author = {S Ternengo and P Katharios}, year = {2008}, date = {2008-10-01}, journal = {Fish Farming Expert}, pages = {44--48}, note = {Place: Bergen, Norway}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Tevdoradze, E; Kokashvili, T; Skhirtladze, N; Balarjishvili, N; Tskhvediani, A; Lashkhi, N; Kutateladze, M; Tediashvili, M; Katharios, P; Papandroulakis, N Isolation and preliminary characterization of Phages specific to Mediterranean fish pathogens. Inproceedings Phage Biology, Ecology and Therapy Meeting, Tbilisi, Georgia, 2008. @inproceedings{tevdoradze_isolation_2008, title = {Isolation and preliminary characterization of Phages specific to Mediterranean fish pathogens.}, author = {E Tevdoradze and T Kokashvili and N Skhirtladze and N Balarjishvili and A Tskhvediani and N Lashkhi and M Kutateladze and M Tediashvili and P Katharios and N Papandroulakis}, year = {2008}, date = {2008-06-01}, booktitle = {Phage Biology, Ecology and Therapy Meeting}, address = {Tbilisi, Georgia}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Katharios, P; Papadaki, M; Papandroulakis, N; Divanach, P Severe mortality in mesocosm-reared sharpsnout sea bream Diplodus puntazzo larvae due to epitheliocystis infection Journal Article Diseases of Aquatic Organisms, 82 (1), pp. 55–60, 2008, ISSN: 01775103. @article{katharios_severe_2008, title = {Severe mortality in mesocosm-reared sharpsnout sea bream Diplodus puntazzo larvae due to epitheliocystis infection}, author = {P Katharios and M Papadaki and N Papandroulakis and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-56649123097&doi=10.3354%2fdao01968&partnerID=40&md5=1c652a653ce36b0e7ae3f6a7b9d1aff6}, doi = {10.3354/dao01968}, issn = {01775103}, year = {2008}, date = {2008-01-01}, journal = {Diseases of Aquatic Organisms}, volume = {82}, number = {1}, pages = {55--60}, abstract = {This paper describes severe mortalities recorded in sharpsnout sea bream Diplodus puntazzo larvae reared in mesocosms. The mortalities were attributed to epitheliocystis infection. The pathology associated with the disease is described using histological techniques. Microscopical examination showed a massive infection of the skin, fins, and oral cavity, with impaired feeding, respiration, and osmoregulation being the most likely cause of death. This is the first report of epitheliocystis disease in sharpsnout sea bream and in fish at such an early developmental stage. © Inter-Research 2008.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This paper describes severe mortalities recorded in sharpsnout sea bream Diplodus puntazzo larvae reared in mesocosms. The mortalities were attributed to epitheliocystis infection. The pathology associated with the disease is described using histological techniques. Microscopical examination showed a massive infection of the skin, fins, and oral cavity, with impaired feeding, respiration, and osmoregulation being the most likely cause of death. This is the first report of epitheliocystis disease in sharpsnout sea bream and in fish at such an early developmental stage. © Inter-Research 2008. |
Papandroulakis, N; Mylonas, C C; Syggelaki, E; Katharios, P; Divanach, P First reproduction of captive-reared wreckfish (Polyprion americanus) using GnRH implants. Inproceedings Aquaculture Europe 08, European Aquaculture Society, Krakow, Poland, 2008, (Backup Publisher: European Aquaculture Society). @inproceedings{papandroulakis_first_2008, title = {First reproduction of captive-reared wreckfish (Polyprion americanus) using GnRH implants.}, author = {N Papandroulakis and C C Mylonas and E Syggelaki and P Katharios and P Divanach}, year = {2008}, date = {2008-01-01}, booktitle = {Aquaculture Europe 08}, publisher = {European Aquaculture Society}, address = {Krakow, Poland}, note = {Backup Publisher: European Aquaculture Society}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Katharios, P; Papandroulakis, N; Papadaki, M; Syggelaki, E; Tzamarias, D; Gounalaki, N; Divanach, P Pseudomonas putida infection in captive wreckfish Polyprion americanus. A case report. Inproceedings Aquaculture Europe 08, pp. 307, European Aquacultrure Society, Krakow, Poland, 2008, (Backup Publisher: European Aquacultrure Society). @inproceedings{katharios_pseudomonas_2008, title = {Pseudomonas putida infection in captive wreckfish Polyprion americanus. A case report.}, author = {P Katharios and N Papandroulakis and M Papadaki and E Syggelaki and D Tzamarias and N Gounalaki and P Divanach}, year = {2008}, date = {2008-01-01}, booktitle = {Aquaculture Europe 08}, pages = {307}, publisher = {European Aquacultrure Society}, address = {Krakow, Poland}, note = {Backup Publisher: European Aquacultrure Society}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
2007 |
Pavlidis, M; Futter, W C; Katharios, P; Divanach, P Blood cell profile of six Mediterranean mariculture fish species Journal Article Journal of Applied Ichthyology, 23 (1), pp. 70–73, 2007, ISSN: 01758659. @article{pavlidis_blood_2007, title = {Blood cell profile of six Mediterranean mariculture fish species}, author = {M Pavlidis and W C Futter and P Katharios and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33846307101&doi=10.1111%2fj.1439-0426.2006.00771.x&partnerID=40&md5=e1768163fb5c96efc554987a9d488e39}, doi = {10.1111/j.1439-0426.2006.00771.x}, issn = {01758659}, year = {2007}, date = {2007-01-01}, journal = {Journal of Applied Ichthyology}, volume = {23}, number = {1}, pages = {70--73}, abstract = {The haematological profile and a description of the cell types from the peripheral blood of six Mediterranean fish species are presented. The highest haematocrit value was recorded in the saupe, Sarpa salpa (P textless 0.001), the only herbivorous species, and which also lacked monocyte cells. Eosinophils were absent from the blood of the European sea bass Dicentrarchus labrax. White sea bream, Diplodus sargus and gilthead sea bream, Sparus aurata had statistically significantly high numbers of neutrophils and low numbers of lymphocytes (P textless 0.001). The numbers of different leucocyte cell types were not influenced by sex or maturity stage in any species, although some variation in the maximum diameter of the cells was observed. © 2007 The Authors Journal compilation © 2007 Blackwell Verlag, Berlin.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The haematological profile and a description of the cell types from the peripheral blood of six Mediterranean fish species are presented. The highest haematocrit value was recorded in the saupe, Sarpa salpa (P textless 0.001), the only herbivorous species, and which also lacked monocyte cells. Eosinophils were absent from the blood of the European sea bass Dicentrarchus labrax. White sea bream, Diplodus sargus and gilthead sea bream, Sparus aurata had statistically significantly high numbers of neutrophils and low numbers of lymphocytes (P textless 0.001). The numbers of different leucocyte cell types were not influenced by sex or maturity stage in any species, although some variation in the maximum diameter of the cells was observed. © 2007 The Authors Journal compilation © 2007 Blackwell Verlag, Berlin. |
Katharios, P; Agathaggelou, A; Paraskevopoulos, S; Mylonas, C C Comparison of iodine and glutaraldehyde as surface disinfectants for red porgy (Pagrus pagrus) and white sea bream (Diplodus sargus sargus) eggs Journal Article Aquaculture Research, 38 (5), pp. 527–536, 2007, ISSN: 1355557X. @article{katharios_comparison_2007, title = {Comparison of iodine and glutaraldehyde as surface disinfectants for red porgy (Pagrus pagrus) and white sea bream (Diplodus sargus sargus) eggs}, author = {P Katharios and A Agathaggelou and S Paraskevopoulos and C C Mylonas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33947541372&doi=10.1111%2fj.1365-2109.2007.01697.x&partnerID=40&md5=ae501174b22223ee369fd8ed85c7cc70}, doi = {10.1111/j.1365-2109.2007.01697.x}, issn = {1355557X}, year = {2007}, date = {2007-01-01}, journal = {Aquaculture Research}, volume = {38}, number = {5}, pages = {527--536}, abstract = {The efficacy of iodine and glutaraldehyde as fish egg surface disinfectants were assessed in red porgy (Pagrus pagrus) and white sea bream (Diplodus sargus sargus) eggs, two species of interest for Mediterranean aquaculture. Iodine was effective in reducing the bacterial load of the 1-day-old eggs when applied at 50 mg L-1 for 5 min. The same concentration did not cause any significant change in hatching success or survival of the larvae for the first 5 days. Glutaraldehyde failed to reduce the bacterial load of the fish eggs at concentrations that were safe for the eggs (100 mg L-1 for 5 min), as it had a significant effect in preventing hatching of the developed embryo. Disinfecting 0-day-old eggs with iodine resulted in a significant reduction of hatching percentage, while larval survival thereafter was unaffected. The results of the present study suggest that iodine may be an appropriate egg disinfectant for both red porgy and white sea bream. © 2007 Blackwell Publishing Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The efficacy of iodine and glutaraldehyde as fish egg surface disinfectants were assessed in red porgy (Pagrus pagrus) and white sea bream (Diplodus sargus sargus) eggs, two species of interest for Mediterranean aquaculture. Iodine was effective in reducing the bacterial load of the 1-day-old eggs when applied at 50 mg L-1 for 5 min. The same concentration did not cause any significant change in hatching success or survival of the larvae for the first 5 days. Glutaraldehyde failed to reduce the bacterial load of the fish eggs at concentrations that were safe for the eggs (100 mg L-1 for 5 min), as it had a significant effect in preventing hatching of the developed embryo. Disinfecting 0-day-old eggs with iodine resulted in a significant reduction of hatching percentage, while larval survival thereafter was unaffected. The results of the present study suggest that iodine may be an appropriate egg disinfectant for both red porgy and white sea bream. © 2007 Blackwell Publishing Ltd. |
Katharios, P; Garaffo, M; Sarter, K; Athanassopoulou, F; Mylonas, C C Bulletin of the European Association of Fish Pathologists, 27 (1), pp. 43–47, 2007, ISSN: 01080288. @article{katharios_case_2007, title = {A case of high mortality due to heavy infestation of Ceratomyxa diplodae in sharpsnout sea bream (Diplodus puntazzo) treated with reproductive steroids}, author = {P Katharios and M Garaffo and K Sarter and F Athanassopoulou and C C Mylonas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-34247115991&partnerID=40&md5=1174ac78ae096f88fc3093f06fc5d4ec}, issn = {01080288}, year = {2007}, date = {2007-01-01}, journal = {Bulletin of the European Association of Fish Pathologists}, volume = {27}, number = {1}, pages = {43--47}, abstract = {A case of 100% mortality in cultured sharpsnout sea bream (Diplodus puntazzo) caused by Ceratomyxa diplodae after sustained steroid treatment for induction of sex inversion is described. The pathology caused by the parasite as well as the possible association of the hormone treatment with the mortality is discussed.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A case of 100% mortality in cultured sharpsnout sea bream (Diplodus puntazzo) caused by Ceratomyxa diplodae after sustained steroid treatment for induction of sex inversion is described. The pathology caused by the parasite as well as the possible association of the hormone treatment with the mortality is discussed. |
2006 |
Katharios, P; Sterioti, A; Divanach, P Cretaquarium: Aquatic health related challenges in setting up a new public aquarium. Inproceedings Fifth International Symposium on Aquatic Animal Health, pp. 147, San Francisco, U.S., 2006. @inproceedings{katharios_cretaquarium_2006, title = {Cretaquarium: Aquatic health related challenges in setting up a new public aquarium.}, author = {P Katharios and A Sterioti and P Divanach}, year = {2006}, date = {2006-09-01}, booktitle = {Fifth International Symposium on Aquatic Animal Health}, pages = {147}, address = {San Francisco, U.S.}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
P., Katharios; N., Papandroulakis; C., Hayward; M., Asderis; Divanach, P Parasitic monogeneans in cultured fish in Crete. Methods for treatment. Inproceedings HCMR, (Ed.): 8th Congress of Oceanography and Fisheries, Thessaloniki, Greece, 2006. @inproceedings{katharios_parasitic_2006, title = {Parasitic monogeneans in cultured fish in Crete. Methods for treatment.}, author = {Katharios P. and Papandroulakis N. and Hayward C. and Asderis M. and P Divanach}, editor = {HCMR}, year = {2006}, date = {2006-06-01}, booktitle = {8th Congress of Oceanography and Fisheries}, address = {Thessaloniki, Greece}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Katharios, P; Papandroulakis, N; Divanach, P Treatment of Microcotyle sp. (Monogenea) on the gills of cage-cultured red porgy, Pagrus pagrus following baths with formalin and mebendazole Journal Article Aquaculture, 251 (2-4), pp. 167–171, 2006, ISSN: 00448486. @article{katharios_treatment_2006, title = {Treatment of Microcotyle sp. (Monogenea) on the gills of cage-cultured red porgy, Pagrus pagrus following baths with formalin and mebendazole}, author = {P Katharios and N Papandroulakis and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-31344445980&doi=10.1016%2fj.aquaculture.2005.05.046&partnerID=40&md5=91ab895aa4134d0252eb1817aaaa56a2}, doi = {10.1016/j.aquaculture.2005.05.046}, issn = {00448486}, year = {2006}, date = {2006-01-01}, journal = {Aquaculture}, volume = {251}, number = {2-4}, pages = {167--171}, abstract = {Two bath treatment trials for the control of the monogenean Microcotyle sp. in the gills of cultured red porgy, Pagrus pagrus using formalin (200 ppm for 1 h) and mebendazole (400 ppm for 1 h) were conducted. Formalin was very effective removing all the parasites from the gills of the infected fish while mebendazole produced no significant result. Based on the results of the trial, formalin was selected for the treatment of a large population of heavily infected red porgies held in sea cages. The results of the treatment are discussed. © 2005 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Two bath treatment trials for the control of the monogenean Microcotyle sp. in the gills of cultured red porgy, Pagrus pagrus using formalin (200 ppm for 1 h) and mebendazole (400 ppm for 1 h) were conducted. Formalin was very effective removing all the parasites from the gills of the infected fish while mebendazole produced no significant result. Based on the results of the trial, formalin was selected for the treatment of a large population of heavily infected red porgies held in sea cages. The results of the treatment are discussed. © 2005 Elsevier B.V. All rights reserved. |
Katharios, P; Hayward, C; Papandroulakis, N; Divanach, P Pathology of Lamellodiscus spp. (Monogenea) parasitizing the gills of sharpsnout seabream and preliminary results of formalin treatment Journal Article Bulletin of the European Association of Fish Pathologists, 26 (5), pp. 196–201, 2006, ISSN: 01080288. @article{katharios_pathology_2006, title = {Pathology of Lamellodiscus spp. (Monogenea) parasitizing the gills of sharpsnout seabream and preliminary results of formalin treatment}, author = {P Katharios and C Hayward and N Papandroulakis and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33845670054&partnerID=40&md5=09814c007ac1f9256a311f1d53bc1f34}, issn = {01080288}, year = {2006}, date = {2006-01-01}, journal = {Bulletin of the European Association of Fish Pathologists}, volume = {26}, number = {5}, pages = {196--201}, abstract = {This report describes the pathological effects Lamellodiscus spp. on the gills of the sharpsnout seabream, Diplodus puntazzo and the efficacy of formalin as a treatment. Two species of Lamellodiscus were identified: Lamellodiscus ergensi and L. bidens. The attachment of parasites caused destruction, fusion and hyperplasia of the gill filaments. Formalin was effective in removing the worms when applied as a bath treatment at a concentration of 200mg/ml for one hour.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This report describes the pathological effects Lamellodiscus spp. on the gills of the sharpsnout seabream, Diplodus puntazzo and the efficacy of formalin as a treatment. Two species of Lamellodiscus were identified: Lamellodiscus ergensi and L. bidens. The attachment of parasites caused destruction, fusion and hyperplasia of the gill filaments. Formalin was effective in removing the worms when applied as a bath treatment at a concentration of 200mg/ml for one hour. |
Katharios, P; Garaffo, M; Sarter, K; Poulou, Athanasso F; Mylonas, C C; Divanach, P Mortality of steroid-treated sharpsnout sea bream Diplodus puntazzo caused by Ceratomyxa diplodae (Protozoa: Myxosporea). Inproceedings Fifth International Symposium on Aquatic Animal Health, pp. 148, San Francisco, U.S., 2006. @inproceedings{katharios_mortality_2006, title = {Mortality of steroid-treated sharpsnout sea bream Diplodus puntazzo caused by Ceratomyxa diplodae (Protozoa: Myxosporea).}, author = {P Katharios and M Garaffo and K Sarter and F Athanasso Poulou and C C Mylonas and P Divanach}, year = {2006}, date = {2006-01-01}, booktitle = {Fifth International Symposium on Aquatic Animal Health}, pages = {148}, address = {San Francisco, U.S.}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
2005 |
Katharios, P; Smullen, R P; Inglis, V The use of the polychaete worm Nereis virens eggs as vehicle for the delivery of oxytetracycline in Solea solea larvae Journal Article Aquaculture, 243 (1-4), pp. 1–7, 2005, ISSN: 00448486. @article{katharios_use_2005, title = {The use of the polychaete worm Nereis virens eggs as vehicle for the delivery of oxytetracycline in Solea solea larvae}, author = {P Katharios and R P Smullen and V Inglis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-10644230309&doi=10.1016%2fj.aquaculture.2004.09.031&partnerID=40&md5=cd90fc4dea25c5202c986ac6b4710655}, doi = {10.1016/j.aquaculture.2004.09.031}, issn = {00448486}, year = {2005}, date = {2005-01-01}, journal = {Aquaculture}, volume = {243}, number = {1-4}, pages = {1--7}, abstract = {The eggs of the polychaete worm Nereis virens were used as a vehicle for the delivery of oxytetracycline (OTC) in Dover sole, Solea solea larvae. The eggs were osmotically loaded, and the level of antibiotic was estimated by HPLC and a microbiological bioassay. The amount of OTC in the N. virens eggs was over 1 μg per egg, which was considered more than sufficient for a therapeutic dose for larval fish. The loaded eggs were tested on the sole larvae, and the results of the experiment (9 μg OTC per fish larva) indicated that the eggs of the Nereis worm can be used as biocarriers of water soluble drugs. © 2004 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The eggs of the polychaete worm Nereis virens were used as a vehicle for the delivery of oxytetracycline (OTC) in Dover sole, Solea solea larvae. The eggs were osmotically loaded, and the level of antibiotic was estimated by HPLC and a microbiological bioassay. The amount of OTC in the N. virens eggs was over 1 μg per egg, which was considered more than sufficient for a therapeutic dose for larval fish. The loaded eggs were tested on the sole larvae, and the results of the experiment (9 μg OTC per fish larva) indicated that the eggs of the Nereis worm can be used as biocarriers of water soluble drugs. © 2004 Elsevier B.V. All rights reserved. |
Katharios, P; Papadakis, I E; Prapas, A; Dermon, C R; Ampatzis, K; Divanach, P Bulletin of the European Association of Fish Pathologists, 25 (1), pp. 28–31, 2005, ISSN: 01080288. @article{katharios_mortality_2005, title = {Mortality control of viral encephalopathy and retinopathy in 0+ grouper Epinephelus marginatus after prolonged bath in dense Chlorella minutissima culture}, author = {P Katharios and I E Papadakis and A Prapas and C R Dermon and K Ampatzis and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-15744380255&partnerID=40&md5=1882681096115ce168df99dfd0aaf0d8}, issn = {01080288}, year = {2005}, date = {2005-01-01}, journal = {Bulletin of the European Association of Fish Pathologists}, volume = {25}, number = {1}, pages = {28--31}, abstract = {0+ grouper (1±0.2g), presenting symptoms of Viral Encephalopathy and Retinopathy (confirmed by isolation of the virus in SSN-1 cell line and identification by IFAT and DNA sequencing as well as by brain histology), were submitted to seven bath treatment regimes for a 30-day period, using different concentrations of Chlorella minutissima and the antibiotic furaltadone. The cumulative mortalities were 0, 0, 5 and 32% when using 2×108, 108, 5×107 and 2×107 cells of C. minutissima ml-1 respectively, compared to 31-36 % in the control and 55-68% in the antibiotic-treated groups. The beneficial effect, the possible immuno-stimulant and antiviral properties of unicellular micro-algae such as C. minutissima, as well as the role of their concentration in the bath treatment, are discussed.}, keywords = {}, pubstate = {published}, tppubtype = {article} } 0+ grouper (1±0.2g), presenting symptoms of Viral Encephalopathy and Retinopathy (confirmed by isolation of the virus in SSN-1 cell line and identification by IFAT and DNA sequencing as well as by brain histology), were submitted to seven bath treatment regimes for a 30-day period, using different concentrations of Chlorella minutissima and the antibiotic furaltadone. The cumulative mortalities were 0, 0, 5 and 32% when using 2×108, 108, 5×107 and 2×107 cells of C. minutissima ml-1 respectively, compared to 31-36 % in the control and 55-68% in the antibiotic-treated groups. The beneficial effect, the possible immuno-stimulant and antiviral properties of unicellular micro-algae such as C. minutissima, as well as the role of their concentration in the bath treatment, are discussed. |
2004 |
P., Katharios; I.E., Papadakis; A., Prapas; C.R., Dermon; K., Ampatzis; Divanach, P Mortality control of VNN disease in 0+ grouper, Epinephelus marginatus after prolonged bath in dense Chlorella minutissima culture. Inproceedings EAS, (Ed.): European Aquaculture Society, Biotechnologies for Quality, Barcelona, Spain, 2004. @inproceedings{katharios_p_mortality_2004, title = {Mortality control of VNN disease in 0+ grouper, Epinephelus marginatus after prolonged bath in dense Chlorella minutissima culture.}, author = {Katharios P. and Papadakis I.E. and Prapas A. and Dermon C.R. and Ampatzis K. and P Divanach}, editor = {EAS}, year = {2004}, date = {2004-10-01}, booktitle = {European Aquaculture Society, Biotechnologies for Quality}, address = {Barcelona, Spain}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Katharios, P; Iliopoulou-Georgudaki, J; Antimisiaris, S; Verri, T; Toma, P; Acierno, R; Maffia, M Pharmacokinetics of cephalexin in sea bream, Sparus aurata (L.), after a single intraperitoneal injection Journal Article Journal of Applied Ichthyology, 20 (5), pp. 422–426, 2004, ISSN: 01758659. @article{katharios_pharmacokinetics_2004, title = {Pharmacokinetics of cephalexin in sea bream, Sparus aurata (L.), after a single intraperitoneal injection}, author = {P Katharios and J Iliopoulou-Georgudaki and S Antimisiaris and T Verri and P Toma and R Acierno and M Maffia}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-5444259750&doi=10.1111%2fj.1439-0426.2004.00578.x&partnerID=40&md5=7ca32ad32162890875c6aca6aff4f216}, doi = {10.1111/j.1439-0426.2004.00578.x}, issn = {01758659}, year = {2004}, date = {2004-01-01}, journal = {Journal of Applied Ichthyology}, volume = {20}, number = {5}, pages = {422--426}, abstract = {The pharmacokinetic properties of cephalexin were studied in sea bream (mean weight 77 g), Sparus aurata (L.) after a single intraperitoneal injection (200 mg kg-1). Pharmacokinetic analysis of the serum concentrations vs time points obtained was performed using non-compartmental analysis and a compartmental pharmacokinetic model approach. In the latter case, a two-compartment open model with a lag time gave the best fitting. The maximum peak serum concentration was 5.018 mg ml-1 kg-1 1 h post-treatment. The area under the curve (AUC) cephalexin was 17.394 mg·h kg-1 and the elimination half-life of 1.83 h. © 2004 Blackwell Verlag, Berlin.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The pharmacokinetic properties of cephalexin were studied in sea bream (mean weight 77 g), Sparus aurata (L.) after a single intraperitoneal injection (200 mg kg-1). Pharmacokinetic analysis of the serum concentrations vs time points obtained was performed using non-compartmental analysis and a compartmental pharmacokinetic model approach. In the latter case, a two-compartment open model with a lag time gave the best fitting. The maximum peak serum concentration was 5.018 mg ml-1 kg-1 1 h post-treatment. The area under the curve (AUC) cephalexin was 17.394 mg·h kg-1 and the elimination half-life of 1.83 h. © 2004 Blackwell Verlag, Berlin. |
Katharios, P; Pavlidis, M; Iliopoulou-Georgudaki, J Accumulation of ivermectin in the brain of sea bream, Sparus aurata after intraperitoneal administration Journal Article Environmental Toxicology and Pharmacology, 17 (1), pp. 9–12, 2004, ISSN: 13826689. @article{katharios_accumulation_2004, title = {Accumulation of ivermectin in the brain of sea bream, Sparus aurata after intraperitoneal administration}, author = {P Katharios and M Pavlidis and J Iliopoulou-Georgudaki}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-2342451297&doi=10.1016%2fj.etap.2004.01.003&partnerID=40&md5=a461dd2f65ec8b5bfa8e6a22e6b2677d}, doi = {10.1016/j.etap.2004.01.003}, issn = {13826689}, year = {2004}, date = {2004-01-01}, journal = {Environmental Toxicology and Pharmacology}, volume = {17}, number = {1}, pages = {9--12}, abstract = {Ivermectin, which is widely used in veterinary and human, has been considered safe due to its inability to penetrate into the central nervous system of higher vertebrates. This paper presents data on the ability of the drug to cross the blood-brain barrier of the marine teleost sea bream, Sparus aurata and accumulate in the brain. The concentration of the drug in the brain and the serum of the fish was assessed by the use of a direct competitive ELISA commercial kit. Our results showed a rapid uptake of the substance by the brain of the fish reaching a maximum concentration of 98.9 ng g-1 8 h post treatment. The trend of the absorption of the drug in brain followed that of the blood. Concentration of the drug in the brain remained high at each sampling point over the 24 h duration of the experiment. In view of these findings, the need of study of the role of the blood-brain barrier and particularly the multidrug resistance mechanism in sea bream is outlined. © 2004 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Ivermectin, which is widely used in veterinary and human, has been considered safe due to its inability to penetrate into the central nervous system of higher vertebrates. This paper presents data on the ability of the drug to cross the blood-brain barrier of the marine teleost sea bream, Sparus aurata and accumulate in the brain. The concentration of the drug in the brain and the serum of the fish was assessed by the use of a direct competitive ELISA commercial kit. Our results showed a rapid uptake of the substance by the brain of the fish reaching a maximum concentration of 98.9 ng g-1 8 h post treatment. The trend of the absorption of the drug in brain followed that of the blood. Concentration of the drug in the brain remained high at each sampling point over the 24 h duration of the experiment. In view of these findings, the need of study of the role of the blood-brain barrier and particularly the multidrug resistance mechanism in sea bream is outlined. © 2004 Elsevier B.V. All rights reserved. |
2003 |
Iliopoulou-Georgudaki, J; Kantzaris, V; Katharios, P; Kaspiris, P; Georgiadis, Th.; Montesantou, B An application of different bioindicators for assessing water quality: A case study in the rivers Alfeios and Pineios (Peloponnisos, Greece) Journal Article Ecological Indicators, 2 (4), pp. 345–360, 2003, ISSN: 1470160X, (Publisher: Elsevier). @article{iliopoulou-georgudaki_application_2003, title = {An application of different bioindicators for assessing water quality: A case study in the rivers Alfeios and Pineios (Peloponnisos, Greece)}, author = {J Iliopoulou-Georgudaki and V Kantzaris and P Katharios and P Kaspiris and Th. Georgiadis and B Montesantou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0141993249&doi=10.1016%2fS1470-160X%2803%2900004-9&partnerID=40&md5=0655df035f5bd0c4f216644b1fe3c623}, doi = {10.1016/S1470-160X(03)00004-9}, issn = {1470160X}, year = {2003}, date = {2003-01-01}, journal = {Ecological Indicators}, volume = {2}, number = {4}, pages = {345--360}, abstract = {A number of bioindicators and biotic indices and scores based on benthic macroinvertebrates, diatoms, fishes, aquatic and riparian vegetation in relation to physicochemical parameters have been applied in assessing the water quality of the rivers Alfeios and Pineios (Peloponnisos, Greece). According to the findings, the water quality in both rivers varied from very poor to very good. Among the bioindicators used, the benthic macroinvertebrates seem to be the most reliable. The BBI and IBE were the most applicable indexes while the applicability of the IBMWP and IASPT in the Greek region can be enforced with the inclusion of the Diptreran family Rhagionidae and the Coleopteran family Elminthidae. © 2003 Published by Elsevier Science Ltd.}, note = {Publisher: Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {article} } A number of bioindicators and biotic indices and scores based on benthic macroinvertebrates, diatoms, fishes, aquatic and riparian vegetation in relation to physicochemical parameters have been applied in assessing the water quality of the rivers Alfeios and Pineios (Peloponnisos, Greece). According to the findings, the water quality in both rivers varied from very poor to very good. Among the bioindicators used, the benthic macroinvertebrates seem to be the most reliable. The BBI and IBE were the most applicable indexes while the applicability of the IBMWP and IASPT in the Greek region can be enforced with the inclusion of the Diptreran family Rhagionidae and the Coleopteran family Elminthidae. © 2003 Published by Elsevier Science Ltd. |
2002 |
Katharios, P; Iliopoulou-Georgudaki, J; Antimisiaris, S; Kantzaris, V; Pavlidis, M Pharmacokinetics of ivermectin in sea bream, Sparus aurata using a direct competitive ELISA Journal Article Fish Physiology and Biochemistry, 26 (2), pp. 189–195, 2002, ISSN: 09201742. @article{katharios_pharmacokinetics_2002, title = {Pharmacokinetics of ivermectin in sea bream, Sparus aurata using a direct competitive ELISA}, author = {P Katharios and J Iliopoulou-Georgudaki and S Antimisiaris and V Kantzaris and M Pavlidis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0141496418&doi=10.1023%2fA%3a1025473904513&partnerID=40&md5=ceed6774234c000f5932e520562473b6}, doi = {10.1023/A:1025473904513}, issn = {09201742}, year = {2002}, date = {2002-01-01}, journal = {Fish Physiology and Biochemistry}, volume = {26}, number = {2}, pages = {189--195}, abstract = {The pharmacokinetics of ivermectin in the serum of cultured sea bream, Sparus aurata, after a single intraperitoneal injection of 100 μg kg-1 body weight was studied by the use of direct competitive ELISA. Pharmacokinetic analysis of the serum concentrations versus time points obtained was performed using non-comparmental analysis and a compartmental pharmacokinetic model approach. In the latter case a two-compartment open model with a lag time gave the best fitting. The maximum peak serum concentration was 308.4 ng ml-1 at 2 h post treatment. The AUC of ivermectin was 10700 ng h ml-1 and the elimination half-life 15.37 h, indicating a rapid uptake, high bioavailability and fast elimination of the drug by sea bream.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The pharmacokinetics of ivermectin in the serum of cultured sea bream, Sparus aurata, after a single intraperitoneal injection of 100 μg kg-1 body weight was studied by the use of direct competitive ELISA. Pharmacokinetic analysis of the serum concentrations versus time points obtained was performed using non-comparmental analysis and a compartmental pharmacokinetic model approach. In the latter case a two-compartment open model with a lag time gave the best fitting. The maximum peak serum concentration was 308.4 ng ml-1 at 2 h post treatment. The AUC of ivermectin was 10700 ng h ml-1 and the elimination half-life 15.37 h, indicating a rapid uptake, high bioavailability and fast elimination of the drug by sea bream. |
Kantzaris, V; Iliopoulou-Georgudaki, J; Katharios, P; Kaspiris, P A comparison of several biotic indices used for water quality assessment at the Greek rivers Journal Article Fresenius Environmental Bulletin, 11 (11), pp. 1000–1007, 2002, ISSN: 10184619. @article{kantzaris_comparison_2002, title = {A comparison of several biotic indices used for water quality assessment at the Greek rivers}, author = {V Kantzaris and J Iliopoulou-Georgudaki and P Katharios and P Kaspiris}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036869322&partnerID=40&md5=63791a418eaa87d734878c737cd2cf66}, issn = {10184619}, year = {2002}, date = {2002-01-01}, journal = {Fresenius Environmental Bulletin}, volume = {11}, number = {11}, pages = {1000--1007}, abstract = {Nine biotic indices and benthic macroinvertebrates scores used for the assessment of water quality over a period of one year at the rivers Kalamas and Alfeios (Greece) were compared. The results of the biotic indexes were combined with the measurements of physicochemical parameters, in order to assess the water quality and to find the most applicable index for assessing Greek rivers. The indexes BBI and IBE were found to be more suitable. However, the need for the establishment of a Greek Biotic Index for assessment of the rivers in the Greek region is marked out.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Nine biotic indices and benthic macroinvertebrates scores used for the assessment of water quality over a period of one year at the rivers Kalamas and Alfeios (Greece) were compared. The results of the biotic indexes were combined with the measurements of physicochemical parameters, in order to assess the water quality and to find the most applicable index for assessing Greek rivers. The indexes BBI and IBE were found to be more suitable. However, the need for the establishment of a Greek Biotic Index for assessment of the rivers in the Greek region is marked out. |
2001 |
I., Leonardos; P., Katharios; Charisis, C Age and growth of Carassius auratus gibelio (Linnaeus, 1758) (Pisces: Cyprinidae) in Lake Lysimachia (W. Greece). Inproceedings of Ichthyologists, Panhellenic Society (Ed.): 10th Panhellenic Conference of Ichthyologists, pp. 257–259, Chania, Greece, 2001. @inproceedings{leonardos_age_2001, title = {Age and growth of Carassius auratus gibelio (Linnaeus, 1758) (Pisces: Cyprinidae) in Lake Lysimachia (W. Greece).}, author = {Leonardos I. and Katharios P. and C Charisis}, editor = {Panhellenic Society of Ichthyologists}, year = {2001}, date = {2001-10-01}, booktitle = {10th Panhellenic Conference of Ichthyologists}, pages = {257--259}, address = {Chania, Greece}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Katharios, P; Iliopoulou-Georgudaki, J; Kapata-Zoumbos, K; Spiropoulos, S Toxicity of intraperitoneally injected ivermectin in sea bream, Sparus aurata Journal Article Fish Physiology and Biochemistry, 25 (2), pp. 99–108, 2001, ISSN: 09201742. @article{katharios_toxicity_2001, title = {Toxicity of intraperitoneally injected ivermectin in sea bream, Sparus aurata}, author = {P Katharios and J Iliopoulou-Georgudaki and K Kapata-Zoumbos and S Spiropoulos}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-14244271014&doi=10.1023%2fA%3a1020574810332&partnerID=40&md5=07b57b30739180b79477443f43f5a1e5}, doi = {10.1023/A:1020574810332}, issn = {09201742}, year = {2001}, date = {2001-01-01}, journal = {Fish Physiology and Biochemistry}, volume = {25}, number = {2}, pages = {99--108}, abstract = {Ivermectin has been widely used as a treatment against sea lice infections of farmed fish (salmonids and non-salmonids) without any official license for use in aquaculture. This study presents data on the toxic side effects of ivermectin administered intraperitoneally in sea bream, Sparus aurata at single doses of 100, 200, 400 and 800 μg ivermectin kg-1 fish. The toxicity of ivermectin was evaluated for a period of 35 days post treatment by determining changes in selected hematological parameters (hematocrit, hemoglobin, red blood cell count, leucocyte count, differential white blood cell count, plasma glucose, electrolytes, urea and alkaline phosphatase), pathological effects in the tissues and the organs of the fish using histological techniques and changes in the behavior of the fish. The results showed a decrease of the hematocrit value one hour after the injection for most of the doses along with an increase of the hemoglobin concentration, the number of the white blood cells, the lymphocytes and monocytes and plasma glucose. No mortality or any histopathological alteration at the tissues examined, were recorded. The fish, which were injected with the highest dose of ivermectin, experienced a loss of appetite, lethargy and darkening of their color during the first week post injection. All the studied parameters reached the normal level by the end of the experiment indicating a low and reversible toxicity of the drug.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Ivermectin has been widely used as a treatment against sea lice infections of farmed fish (salmonids and non-salmonids) without any official license for use in aquaculture. This study presents data on the toxic side effects of ivermectin administered intraperitoneally in sea bream, Sparus aurata at single doses of 100, 200, 400 and 800 μg ivermectin kg-1 fish. The toxicity of ivermectin was evaluated for a period of 35 days post treatment by determining changes in selected hematological parameters (hematocrit, hemoglobin, red blood cell count, leucocyte count, differential white blood cell count, plasma glucose, electrolytes, urea and alkaline phosphatase), pathological effects in the tissues and the organs of the fish using histological techniques and changes in the behavior of the fish. The results showed a decrease of the hematocrit value one hour after the injection for most of the doses along with an increase of the hemoglobin concentration, the number of the white blood cells, the lymphocytes and monocytes and plasma glucose. No mortality or any histopathological alteration at the tissues examined, were recorded. The fish, which were injected with the highest dose of ivermectin, experienced a loss of appetite, lethargy and darkening of their color during the first week post injection. All the studied parameters reached the normal level by the end of the experiment indicating a low and reversible toxicity of the drug. |
2000 |
P., Katharios; Κ., Kapata-Zoumbou; Iliopoulou-Georgudaki, J Toxicity of intraperitoneal administered ivermectin in sea bream, Sparus aurata. Inproceedings of Sciences, Hellenic Society Biological (Ed.): pp. 56–60, Chios, Greece, 2000. @inproceedings{katharios_toxicity_2000, title = {Toxicity of intraperitoneal administered ivermectin in sea bream, Sparus aurata.}, author = {Katharios P. and Kapata-Zoumbou Κ. and J Iliopoulou-Georgudaki}, editor = {Hellenic Society Biological of Sciences}, year = {2000}, date = {2000-01-01}, pages = {56--60}, address = {Chios, Greece}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
1999 |
J., Iliopoulou-Georgudaki; P., Kaspiris; V., Kantzaris; Katharios, P Study of the seasonal fluctuation of the ecological quality of the Rivers: Kalamas, Acherontas, Alfios, Pinios with the use of benthic macro-invertebrates as bioindicators. Inproceedings of Sciences, Hellenic Society Biological (Ed.): 21st Conference of the Hellenic Society of Biological Sciences, pp. 91–92, Syros, Greece, 1999. @inproceedings{iliopoulou-georgudaki_study_1999, title = {Study of the seasonal fluctuation of the ecological quality of the Rivers: Kalamas, Acherontas, Alfios, Pinios with the use of benthic macro-invertebrates as bioindicators.}, author = {Iliopoulou-Georgudaki J. and Kaspiris P. and Kantzaris V. and P Katharios}, editor = {Hellenic Society Biological of Sciences}, year = {1999}, date = {1999-01-01}, booktitle = {21st Conference of the Hellenic Society of Biological Sciences}, pages = {91--92}, address = {Syros, Greece}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |