2024 |
Triga, Adriana; Issa, Zeenat Atinuke; Smyrli, Maria; Fenske, Linda; Katharios, Pantelis Virulence and pangenome analysis of Vibrio harveyi strains from Greek and Red Sea marine aquaculture Journal Article Aquaculture, 587 , pp. 740839, 2024, ISSN: 00448486. @article{triga_virulence_2024, title = {Virulence and pangenome analysis of Vibrio harveyi strains from Greek and Red Sea marine aquaculture}, author = {Adriana Triga and Zeenat Atinuke Issa and Maria Smyrli and Linda Fenske and Pantelis Katharios}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2024/04/2024-Triga-AQUA-preprint-22.pdf https://linkinghub.elsevier.com/retrieve/pii/S0044848624003004}, doi = {10.1016/j.aquaculture.2024.740839}, issn = {00448486}, year = {2024}, date = {2024-04-19}, urldate = {2024-04-19}, journal = {Aquaculture}, volume = {587}, pages = {740839}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2023 |
Triga, Adriana; Smyrli, Maria; Katharios, Pantelis Microorganisms, 11 (5), pp. 1197, 2023, ISSN: 2076-2607. @article{triga_pathogenic_2023, title = {Pathogenic and Opportunistic Vibrio spp. Associated with Vibriosis Incidences in the Greek Aquaculture: The Role of Vibrio harveyi as the Principal Cause of Vibriosis}, author = {Adriana Triga and Maria Smyrli and Pantelis Katharios}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/05/2023-Triga-microorganisms-19.pdf https://www.mdpi.com/2076-2607/11/5/1197}, doi = {10.3390/microorganisms11051197}, issn = {2076-2607}, year = {2023}, date = {2023-05-17}, urldate = {2023-05-17}, journal = {Microorganisms}, volume = {11}, number = {5}, pages = {1197}, abstract = {A monitoring program to follow vibriosis incidents in the Greek marine aquaculture was implemented over the past 13 years. 273 isolates, from various cases originating from eight regions and nine hosts, were collected and characterized. The main aquaculture species of the survey were the European seabass (Dicentrarchus labrax) and the gilthead seabream (Sparus aurata). Various species of Vibrionaceae were associated with vibriosis. Vibrio harveyi had the highest prevalence and was isolated throughout the year from all hosts. During the warm months, Vibrio harveyi prevailed with frequent co-isolations of Photobacterium damselae subsp. damselae and Vibrio alginolyticus, while during spring, other Vibrio species were more abundant, such as Vibrio lentus, Vibrio cyclitrophicus, and Vibrio gigantis. Phylogenetic analysis using the mreB gene and the metabolic fingerprint of the isolates showed great variability within the species of the collection. The severity of the disease and the frequency of outbreaks make vibriosis (that is, mainly attributed to V. harveyi) an important concern for the regional aquaculture sector.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A monitoring program to follow vibriosis incidents in the Greek marine aquaculture was implemented over the past 13 years. 273 isolates, from various cases originating from eight regions and nine hosts, were collected and characterized. The main aquaculture species of the survey were the European seabass (Dicentrarchus labrax) and the gilthead seabream (Sparus aurata). Various species of Vibrionaceae were associated with vibriosis. Vibrio harveyi had the highest prevalence and was isolated throughout the year from all hosts. During the warm months, Vibrio harveyi prevailed with frequent co-isolations of Photobacterium damselae subsp. damselae and Vibrio alginolyticus, while during spring, other Vibrio species were more abundant, such as Vibrio lentus, Vibrio cyclitrophicus, and Vibrio gigantis. Phylogenetic analysis using the mreB gene and the metabolic fingerprint of the isolates showed great variability within the species of the collection. The severity of the disease and the frequency of outbreaks make vibriosis (that is, mainly attributed to V. harveyi) an important concern for the regional aquaculture sector. |
2022 |
Smyrli, Maria; Anka, Ishrat Zahan; Koutsoni, Olga; Dotsika, Eleni; Kyriazis, Ioannis D; Pavlidis, Michail; Katharios, Pantelis Development of autogenous vaccines for farmed European seabass against Aeromonas veronii using zebrafish as a model for efficacy assessment Journal Article Fish & Shellfish Immunology, 123 , pp. 381–387, 2022, ISSN: 10504648. @article{smyrli_development_2022, title = {Development of autogenous vaccines for farmed European seabass against Aeromonas veronii using zebrafish as a model for efficacy assessment}, author = {Maria Smyrli and Ishrat Zahan Anka and Olga Koutsoni and Eleni Dotsika and Ioannis D Kyriazis and Michail Pavlidis and Pantelis Katharios}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/05/2022-Smyrli-FSImmune-24-pre-print.pdf https://linkinghub.elsevier.com/retrieve/pii/S1050464822001450}, doi = {10.1016/j.fsi.2022.03.019}, issn = {10504648}, year = {2022}, date = {2022-04-01}, urldate = {2022-05-09}, journal = {Fish & Shellfish Immunology}, volume = {123}, pages = {381--387}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Triga, Adriana; Smyrli, Maria; Katharios, Pantelis Aeromoniasis Incollection Aquaculture Pathophysiology, pp. 317–327, Elsevier, 2022, ISBN: 9780128122112. @incollection{triga_aeromoniasis_2022, title = {Aeromoniasis}, author = {Adriana Triga and Maria Smyrli and Pantelis Katharios}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/10/2022-Tringa-chapter-25-Aeromonas-preprint-68.pdf https://linkinghub.elsevier.com/retrieve/pii/B978012812211200024X}, doi = {10.1016/B978-0-12-812211-2.00024-X}, isbn = {9780128122112}, year = {2022}, date = {2022-01-01}, urldate = {2022-10-12}, booktitle = {Aquaculture Pathophysiology}, pages = {317--327}, publisher = {Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {incollection} } |
2020 |
Smyrli, Maria; Katharios, Pantelis Aeromonas spp. Incollection Zrnčić, Snježana (Ed.): Diagnostic manual for the main pathogens in European seabass and Gilthead seabream aquaculture, (75), pp. 107–116, CIHEAM, Zaragoza, 2020, ISBN: 978-2-85352-596-1. @incollection{smyrli_aeromonas_2020, title = {Aeromonas spp.}, author = {Maria Smyrli and Pantelis Katharios}, editor = {Snježana Zrnčić}, url = {http://om.ciheam.org/om/pdf/b75/b75.pdf}, isbn = {978-2-85352-596-1}, year = {2020}, date = {2020-01-01}, booktitle = {Diagnostic manual for the main pathogens in European seabass and Gilthead seabream aquaculture}, number = {75}, pages = {107--116}, publisher = {CIHEAM}, address = {Zaragoza}, series = {Options Méditerranéennes, Series B: Studies and Research}, keywords = {}, pubstate = {published}, tppubtype = {incollection} } |
2019 |
Smyrli, Maria; Triga, Adriana; Dourala, Nancy; Varvarigos, Panos; Pavlidis, Michael; Quoc, Viet Ha; Katharios, Pantelis Comparative study on a novel pathogen of european seabass. Diversity of Aeromonas veronii in the aegean sea Journal Article Microorganisms, 7 (11), 2019, ISSN: 20762607. @article{smyrli_comparative_2019, title = {Comparative study on a novel pathogen of european seabass. Diversity of Aeromonas veronii in the aegean sea}, author = {Maria Smyrli and Adriana Triga and Nancy Dourala and Panos Varvarigos and Michael Pavlidis and Viet Ha Quoc and Pantelis Katharios}, doi = {10.3390/microorganisms7110504}, issn = {20762607}, year = {2019}, date = {2019-01-01}, journal = {Microorganisms}, volume = {7}, number = {11}, abstract = {Aeromonas veronii is an emerging pathogen causing severe pathology and mortalities in European seabass aquaculture in the Aegean Sea, Mediterranean. More than 50 strains of the pathogen were characterized biochemically and genetically in order to study the epidemiology of the disease, as well as the phylogeny and virulence of the bacterium. Based on the phenotypic characteristics, the isolates form three groups consisting of: (a) the West Aegean Sea, non-motile, non-pigment-producing strains, (b) the West Aegean Sea, motile, and pigment-producing strains and (c) the East Aegean Sea motile strains that produce minute amounts of pigment. All strains were highly similar at the genomic level; however, the pattern of West/East geographic origin was reflected in biochemical properties, in general genomic level comparison and in the putative virulent factors studied. Type VI secretion system was not detected in the western strains. The outer membrane protein (OMP) profile which contains proteins that are putative antigenic factors, was very similar between strains from the different areas. Although most of the OMPs were detected in all strains with great sequence similarity, diversification according to geographic origin was evident in known antigenic factors such as the maltoporin LamB. A systematic comparative analysis of the strains is presented and discussed in view of the emergence of A. veronii as a significant pathogen for the Mediterranean aquaculture.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Aeromonas veronii is an emerging pathogen causing severe pathology and mortalities in European seabass aquaculture in the Aegean Sea, Mediterranean. More than 50 strains of the pathogen were characterized biochemically and genetically in order to study the epidemiology of the disease, as well as the phylogeny and virulence of the bacterium. Based on the phenotypic characteristics, the isolates form three groups consisting of: (a) the West Aegean Sea, non-motile, non-pigment-producing strains, (b) the West Aegean Sea, motile, and pigment-producing strains and (c) the East Aegean Sea motile strains that produce minute amounts of pigment. All strains were highly similar at the genomic level; however, the pattern of West/East geographic origin was reflected in biochemical properties, in general genomic level comparison and in the putative virulent factors studied. Type VI secretion system was not detected in the western strains. The outer membrane protein (OMP) profile which contains proteins that are putative antigenic factors, was very similar between strains from the different areas. Although most of the OMPs were detected in all strains with great sequence similarity, diversification according to geographic origin was evident in known antigenic factors such as the maltoporin LamB. A systematic comparative analysis of the strains is presented and discussed in view of the emergence of A. veronii as a significant pathogen for the Mediterranean aquaculture. |
2018 |
Tsertou, M I; Smyrli, M; Kokkari, C; Antonopoulou, E; Katharios, P The aetiology of systemic granulomatosis in meagre (Argyrosomus regius): The “Nocardia” hypothesis Journal Article Aquaculture Reports, 12 , pp. 5–11, 2018, ISSN: 23525134, (Publisher: Elsevier B.V.). @article{tsertou_aetiology_2018, title = {The aetiology of systemic granulomatosis in meagre (Argyrosomus regius): The “Nocardia” hypothesis}, author = {M I Tsertou and M Smyrli and C Kokkari and E Antonopoulou and P Katharios}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85052467668&doi=10.1016%2fj.aqrep.2018.08.002&partnerID=40&md5=625a13223330d31956144851287ee794}, doi = {10.1016/j.aqrep.2018.08.002}, issn = {23525134}, year = {2018}, date = {2018-01-01}, journal = {Aquaculture Reports}, volume = {12}, pages = {5--11}, abstract = {One of the most important bottlenecks of meagre (Argyrosomus regius) production is Systemic Granulomatosis (SG), a pathological condition affecting the majority of farmed populations. The aetiology of the disease, which is characterized by multiple granulomas in all soft tissues, is unknown; however, two hypotheses have been raised. The first is that it may be a metabolic disorder similar to systemic granulomas observed in other cultured fishes. The second hypothesis is that it is caused by bacterial pathogens, most likely Nocardia spp. (i.e. nocardiosis). The aim of this study was to test the bacterial pathogen hypothesis as the aetiological factor of SG. Meagre from various locations of Greece were sampled and examined using microbiological, molecular and histological techniques. The prevalence of granulomas in fish was 100% and was not correlated to an infectious agent, whereas nocardiosis was found in a single incidence (prevalence: 1.3%). The results suggest that nocardiosis is not the cause of SG, as it is most probably present in a confined geographical region in Greece. The metabolic disorder hypothesis for SG is more probable and more research has to been done to conclusively identify the aetiological factor of SG. © 2018 The Authors}, note = {Publisher: Elsevier B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } One of the most important bottlenecks of meagre (Argyrosomus regius) production is Systemic Granulomatosis (SG), a pathological condition affecting the majority of farmed populations. The aetiology of the disease, which is characterized by multiple granulomas in all soft tissues, is unknown; however, two hypotheses have been raised. The first is that it may be a metabolic disorder similar to systemic granulomas observed in other cultured fishes. The second hypothesis is that it is caused by bacterial pathogens, most likely Nocardia spp. (i.e. nocardiosis). The aim of this study was to test the bacterial pathogen hypothesis as the aetiological factor of SG. Meagre from various locations of Greece were sampled and examined using microbiological, molecular and histological techniques. The prevalence of granulomas in fish was 100% and was not correlated to an infectious agent, whereas nocardiosis was found in a single incidence (prevalence: 1.3%). The results suggest that nocardiosis is not the cause of SG, as it is most probably present in a confined geographical region in Greece. The metabolic disorder hypothesis for SG is more probable and more research has to been done to conclusively identify the aetiological factor of SG. © 2018 The Authors |
2017 |
Smyrli, M; Prapas, A; Rigos, G; Kokkari, C; Pavlidis, M; Katharios, P Fish Pathology, 52 (2), pp. 68–81, 2017, ISSN: 0388788X, (Publisher: Japanese Society of Fish Pathology). @article{smyrli_aeromonas_2017, title = {Aeromonas veronii infection associated with high morbidity and mortality in farmed European seabass Dicentrarchus labrax in the Aegean Sea, Greece}, author = {M Smyrli and A Prapas and G Rigos and C Kokkari and M Pavlidis and P Katharios}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85022184875&doi=10.3147%2fjsfp.52.68&partnerID=40&md5=d4e9a7e3342b0d17ae8291af235a0206}, doi = {10.3147/jsfp.52.68}, issn = {0388788X}, year = {2017}, date = {2017-01-01}, journal = {Fish Pathology}, volume = {52}, number = {2}, pages = {68--81}, abstract = {In the present study, we examined a disease caused by Aeromonas veronii bv. sobria in sea cage-farmed European seabass Dicentrarchus labrax, in the Aegean Sea, Greece. Commercial sized fish were affected by A. veronii bv. sobria and exhibited high morbidity and mortality. Gross pathologic features and histology revealed a systemic infection characterized by the presence of abscesses and chronic granulomatous inflammation. Two clinical bacterial isolates (Aero NS and Aero PDB) were identified as A. veronii bv. sobria based on bacteriological characteristics and sequence analysis for 16S rRNA and gyrB genes. Infectivity tests in the form of intraperitoneal injection administration (Aero NS) and immersion in a bacterial suspension (Aero NS and Aero PDB) revealed that both isolates could cause clinical signs similar to those observed in the field and high mortality rate. To our knowledge, this is the first report of A. veronii bv. sobria isolated from farmed European seabass in the Mediterranean Sea accompanied by supporting data of its pathogenicity. © 2017 The Japanese Society of Fish Pathology.}, note = {Publisher: Japanese Society of Fish Pathology}, keywords = {}, pubstate = {published}, tppubtype = {article} } In the present study, we examined a disease caused by Aeromonas veronii bv. sobria in sea cage-farmed European seabass Dicentrarchus labrax, in the Aegean Sea, Greece. Commercial sized fish were affected by A. veronii bv. sobria and exhibited high morbidity and mortality. Gross pathologic features and histology revealed a systemic infection characterized by the presence of abscesses and chronic granulomatous inflammation. Two clinical bacterial isolates (Aero NS and Aero PDB) were identified as A. veronii bv. sobria based on bacteriological characteristics and sequence analysis for 16S rRNA and gyrB genes. Infectivity tests in the form of intraperitoneal injection administration (Aero NS) and immersion in a bacterial suspension (Aero NS and Aero PDB) revealed that both isolates could cause clinical signs similar to those observed in the field and high mortality rate. To our knowledge, this is the first report of A. veronii bv. sobria isolated from farmed European seabass in the Mediterranean Sea accompanied by supporting data of its pathogenicity. © 2017 The Japanese Society of Fish Pathology. |
2015 |
Katharios, P; Kokkari, C; Dourala, N; Smyrli, M First report of Edwardsiellosis in cage-cultured sharpsnout sea bream, Diplodus puntazzo from the Mediterranean Journal Article BMC Veterinary Research, 11 (1), pp. 155, 2015, ISSN: 17466148, (Publisher: BioMed Central Ltd.). @article{katharios_first_2015, title = {First report of Edwardsiellosis in cage-cultured sharpsnout sea bream, Diplodus puntazzo from the Mediterranean}, author = {P Katharios and C Kokkari and N Dourala and M Smyrli}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84937551746&doi=10.1186%2fs12917-015-0482-x&partnerID=40&md5=a7c848edbc8a8ac0151f14734aabb8cf}, doi = {10.1186/s12917-015-0482-x}, issn = {17466148}, year = {2015}, date = {2015-01-01}, journal = {BMC Veterinary Research}, volume = {11}, number = {1}, pages = {155}, abstract = {Background: Edwardsiella tarda, is a serious bacterial pathogen affecting a broad range of aquaculture fish species. The bacterium has also been reported as a human pathogen, however recent studies have dissociated the fish pathogenic Edwardsiella from those isolated from humans by placing them in a new species, E. piscicida. Here we report the first case of Edwardsiellosis in cultured sharpsnout sea breams, Diplodus puntazzo in Greece. Case presentation: The disease has affected cultured sharpsnout sea breams of a commercial fish farm in a single location in East Greece. Two populations of sharpsnout sea breams stocked in two consecutive years in floating cages presented signs of disease which included nodules and abscesses in spleen and kidney, morbidity and cumulative mortality reaching 5.3 %. Using microbiological, biochemical and molecular tools we have identified Edwardsiella sp. as the main aetiological factor of the disease. Following phylogenetic analysis the bacterial isolates are grouped with the newly described Edwardsiella piscicida species. Conclusions: This is the first report of Edwardsiellosis in this species but most importantly in sea cage-cultured fish in the Mediterranean which may pose a serious threat for aquaculture fish species in this region. © 2015 Katharios et al.}, note = {Publisher: BioMed Central Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Background: Edwardsiella tarda, is a serious bacterial pathogen affecting a broad range of aquaculture fish species. The bacterium has also been reported as a human pathogen, however recent studies have dissociated the fish pathogenic Edwardsiella from those isolated from humans by placing them in a new species, E. piscicida. Here we report the first case of Edwardsiellosis in cultured sharpsnout sea breams, Diplodus puntazzo in Greece. Case presentation: The disease has affected cultured sharpsnout sea breams of a commercial fish farm in a single location in East Greece. Two populations of sharpsnout sea breams stocked in two consecutive years in floating cages presented signs of disease which included nodules and abscesses in spleen and kidney, morbidity and cumulative mortality reaching 5.3 %. Using microbiological, biochemical and molecular tools we have identified Edwardsiella sp. as the main aetiological factor of the disease. Following phylogenetic analysis the bacterial isolates are grouped with the newly described Edwardsiella piscicida species. Conclusions: This is the first report of Edwardsiellosis in this species but most importantly in sea cage-cultured fish in the Mediterranean which may pose a serious threat for aquaculture fish species in this region. © 2015 Katharios et al. |
2014 |
Katharios, P; Kokkari, C; Sterioti, A; Smyrli, M; Kalatzis, P G Enteromyxum leei infection in parrotfish, Sparisoma cretense: Histopathological, morphological and molecular study Journal Article Veterinary Parasitology, 199 (3-4), pp. 136–143, 2014, ISSN: 03044017. @article{katharios_enteromyxum_2014, title = {Enteromyxum leei infection in parrotfish, Sparisoma cretense: Histopathological, morphological and molecular study}, author = {P Katharios and C Kokkari and A Sterioti and M Smyrli and P G Kalatzis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84890553160&doi=10.1016%2fj.vetpar.2013.10.011&partnerID=40&md5=f9944a453e454ecfa3c4431daeb37632}, doi = {10.1016/j.vetpar.2013.10.011}, issn = {03044017}, year = {2014}, date = {2014-01-01}, journal = {Veterinary Parasitology}, volume = {199}, number = {3-4}, pages = {136--143}, abstract = {Enteromyxum leei (Myxozoa) has been identified as the aetiological agent of morbidity and mortality in captive feral parrotfish, Sparisoma cretense held at the facilities of Cretaquarium, the public aquarium of the Hellenic Centre for Marine Research in Crete. The parasite caused substantial mortality to the parrotfish exceeding 60% over a period of 3.5 months. Here we describe the course of disease, the effects of the parasite on the host based on histopathological observations and we provide morphological and molecular data on the parasite. © 2013 Elsevier B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Enteromyxum leei (Myxozoa) has been identified as the aetiological agent of morbidity and mortality in captive feral parrotfish, Sparisoma cretense held at the facilities of Cretaquarium, the public aquarium of the Hellenic Centre for Marine Research in Crete. The parasite caused substantial mortality to the parrotfish exceeding 60% over a period of 3.5 months. Here we describe the course of disease, the effects of the parasite on the host based on histopathological observations and we provide morphological and molecular data on the parasite. © 2013 Elsevier B.V. |
2011 |
González-Solís, J; Smyrli, M; Militão, T; Gremillet, D; Tveraa, T; Phillips, Ra; Boulinier, T Combining stable isotope analyses and geolocation to reveal kittiwake migration Journal Article Marine Ecology Progress Series, 435 , pp. 251–261, 2011, ISSN: 0171-8630, 1616-1599. @article{gonzalez-solis_combining_2011, title = {Combining stable isotope analyses and geolocation to reveal kittiwake migration}, author = {J González-Solís and M Smyrli and T Militão and D Gremillet and T Tveraa and Ra Phillips and T Boulinier}, url = {http://www.int-res.com/abstracts/meps/v435/p251-261/}, doi = {10.3354/meps09233}, issn = {0171-8630, 1616-1599}, year = {2011}, date = {2011-08-01}, urldate = {2020-09-14}, journal = {Marine Ecology Progress Series}, volume = {435}, pages = {251--261}, keywords = {}, pubstate = {published}, tppubtype = {article} } |