2025
Lancerotto, Stefano; Fakriadis, Ioannis; Papadaki, Maria; Sigelaki, Irini; Giménez, Ignacio; Genoves, Jose Vicente Roig; Meloni, Andrea; Mylonas, Constantinos C
In: Aquaculture, vol. 609, pp. 742795, 2025, ISSN: 00448486.
@article{lancerotto_administration_2025,
title = {Administration of single-chain recombinant follicle-stimulating hormone (sdrFsh) and luteinizing hormone (sdrLh) stimulates spermatogenesis, but not vitellogenesis in pre-pubertal greater amberjack (\textit{Seriola dumerili})},
author = {Stefano Lancerotto and Ioannis Fakriadis and Maria Papadaki and Irini Sigelaki and Ignacio Giménez and Jose Vicente Roig Genoves and Andrea Meloni and Constantinos C Mylonas},
url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848625006817
/wp-content/uploads/2025/06/2025-Lancerotto-AQUA-pre-print32.pdf},
doi = {10.1016/j.aquaculture.2025.742795},
issn = {00448486},
year = {2025},
date = {2025-06-24},
urldate = {2025-06-24},
journal = {Aquaculture},
volume = {609},
pages = {742795},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Lancerotto, Stefano; Fakriadis, Ioannis; Papadaki, Maria; Giménez, Ignacio; Genovés, José Vicente Roig; Mylonas, Constantinos C
In: Aquaculture, vol. 594, pp. 741401, 2025, ISSN: 00448486.
@article{lancerotto_overcoming_2025,
title = {Overcoming dysfunctional gametogenesis in hatchery-produced greater amberjack, Seriola dumerili using recombinant gonadotropins, and spawning induction using gonadotropin releasing hormone agonist-loaded implants},
author = {Stefano Lancerotto and Ioannis Fakriadis and Maria Papadaki and Ignacio Giménez and José Vicente Roig Genovés and Constantinos C Mylonas},
url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848624008627
https://imbbc.hcmr.gr/wp-content/uploads/2025/01/2025-Lancerotto-AQUA-2.pdf},
doi = {10.1016/j.aquaculture.2024.741401},
issn = {00448486},
year = {2025},
date = {2025-01-21},
urldate = {2025-01-21},
journal = {Aquaculture},
volume = {594},
pages = {741401},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
2024
Superio, Joshua; Resseguier, Julien; Nobrega, Rafael Henrique; Grebstad, Caroline M; Fakriadis, Ioannis; Foss, Atle; Hagen, Ørjan; Zhang, Meiling; García-Hernández, Maria Del Pilar; Galindo-Villegas, Jorge
In: Aquaculture, vol. 592, pp. 741214, 2024, ISSN: 00448486.
@article{superio_unravelling_2024,
title = {Unravelling spermatogenesis in spotted wolffish: Insights from the ultrastructure of juvenile male testes to the cryopreservation of broodstock sperm},
author = {Joshua Superio and Julien Resseguier and Rafael Henrique Nobrega and Caroline M Grebstad and Ioannis Fakriadis and Atle Foss and Ørjan Hagen and Meiling Zhang and Maria Del Pilar García-Hernández and Jorge Galindo-Villegas},
url = {https://imbbc.hcmr.gr/wp-content/uploads/2024/07/2024-Superio-AQUA-30.pdf
https://linkinghub.elsevier.com/retrieve/pii/S0044848624006756},
doi = {10.1016/j.aquaculture.2024.741214},
issn = {00448486},
year = {2024},
date = {2024-07-01},
urldate = {2024-07-01},
journal = {Aquaculture},
volume = {592},
pages = {741214},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Papadaki, Maria; Karamanlidis, Dimitris; Sigelaki, Eirini; Fakriadis, Ioannis; Mylonas, Constantinos C
Evolution of sex ratio and egg production of gilthead seabream (Sparus aurata) over the course of five reproductive seasons Journal Article
In: Aquaculture and Fisheries, vol. 9, no. 4, pp. 534–542, 2024, ISSN: 2468550X.
@article{papadaki_evolution_2024,
title = {Evolution of sex ratio and egg production of gilthead seabream (Sparus aurata) over the course of five reproductive seasons},
author = {Maria Papadaki and Dimitris Karamanlidis and Eirini Sigelaki and Ioannis Fakriadis and Constantinos C Mylonas},
url = {https://imbbc.hcmr.gr/wp-content/uploads/2024/06/2024-Papadaki-AquaFish-27.pdf
https://linkinghub.elsevier.com/retrieve/pii/S2468550X22001836},
doi = {10.1016/j.aaf.2022.10.006},
issn = {2468550X},
year = {2024},
date = {2024-06-13},
urldate = {2024-06-13},
journal = {Aquaculture and Fisheries},
volume = {9},
number = {4},
pages = {534--542},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Katirtzoglou, Aikaterini; Tsaparis, Dimitris; Kolios, Evangelos; Magoulas, Antonios; Mylonas, Constantinos C; Fakriadis, Ioannis; Manousaki, Tereza; Tsigenopoulos, Costas S
In: Frontiers in Fish Science, vol. 2, pp. 1356313, 2024, ISSN: 2813-9097.
@article{katirtzoglou_population_2024,
title = {Population genomic analysis of the greater amberjack (\textit{Seriola dumerili}) in the Mediterranean and the Northeast Atlantic, based on SNPs, microsatellites, and mitochondrial DNA sequences},
author = {Aikaterini Katirtzoglou and Dimitris Tsaparis and Evangelos Kolios and Antonios Magoulas and Constantinos C Mylonas and Ioannis Fakriadis and Tereza Manousaki and Costas S Tsigenopoulos},
url = {https://imbbc.hcmr.gr/wp-content/uploads/2024/03/2024-Kaitirtzoglou-frish-14.pdf
https://www.frontiersin.org/articles/10.3389/frish.2024.1356313/full},
doi = {10.3389/frish.2024.1356313},
issn = {2813-9097},
year = {2024},
date = {2024-03-14},
urldate = {2024-03-14},
journal = {Frontiers in Fish Science},
volume = {2},
pages = {1356313},
abstract = {The greater amberjack (
Seriola dumerili
) has attracted considerable economic interest since the 1990s because it possesses traits that distinguish it as a promising candidate in aquaculture. However, the challenges of its successful rearing, combined with the need to develop proper management practices, underline the importance of research into the genetic diversity of both wild and farmed stocks. We aimed to decipher the genetic structure of
S. dumerili
in the Eastern Mediterranean and the Northeast Atlantic using three types of genetic markers (mitochondrial control region sequences, microsatellites, and single nucleotide polymorphisms, or SNPs) to evaluate the population genetic structure of the species. The results were compared to those of previous studies of the species focusing on the Central Mediterranean and confirmed the presence of two divergent mtDNA haplogroups distributed without any detectable geographical structuring within the Mediterranean and the Atlantic. However, population analyses of the genetic structure of the species using either 10 microsatellites or a set of 1,051 SNP markers suggest that the species forms three genetically distinct groups, one in the Mediterranean and two in the Atlantic. The latter has so far not been reported in the Northeast Atlantic, and that this differentiation refers to samples from the Canary Islands is surprising. Fifteen candidate outlier SNP loci were identified in the data set, one located within the
tead1a
/TEF-1A gene, which has been associated with temperature acclimation of the medaka. This work enriches our knowledge of the genetic diversity of wild populations of the greater amberjack in the Mediterranean and the Northeast Atlantic and attempts to investigate signs of local adaptation toward a better understanding of the species distribution patterns. Future studies should be conducted to investigate the genomic regions associated with temperature acclimation in marine organisms that have to adapt to the ongoing climate change pressures, such as sea temperature rise, in order to survive.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The greater amberjack (
Seriola dumerili
) has attracted considerable economic interest since the 1990s because it possesses traits that distinguish it as a promising candidate in aquaculture. However, the challenges of its successful rearing, combined with the need to develop proper management practices, underline the importance of research into the genetic diversity of both wild and farmed stocks. We aimed to decipher the genetic structure of
S. dumerili
in the Eastern Mediterranean and the Northeast Atlantic using three types of genetic markers (mitochondrial control region sequences, microsatellites, and single nucleotide polymorphisms, or SNPs) to evaluate the population genetic structure of the species. The results were compared to those of previous studies of the species focusing on the Central Mediterranean and confirmed the presence of two divergent mtDNA haplogroups distributed without any detectable geographical structuring within the Mediterranean and the Atlantic. However, population analyses of the genetic structure of the species using either 10 microsatellites or a set of 1,051 SNP markers suggest that the species forms three genetically distinct groups, one in the Mediterranean and two in the Atlantic. The latter has so far not been reported in the Northeast Atlantic, and that this differentiation refers to samples from the Canary Islands is surprising. Fifteen candidate outlier SNP loci were identified in the data set, one located within the
tead1a
/TEF-1A gene, which has been associated with temperature acclimation of the medaka. This work enriches our knowledge of the genetic diversity of wild populations of the greater amberjack in the Mediterranean and the Northeast Atlantic and attempts to investigate signs of local adaptation toward a better understanding of the species distribution patterns. Future studies should be conducted to investigate the genomic regions associated with temperature acclimation in marine organisms that have to adapt to the ongoing climate change pressures, such as sea temperature rise, in order to survive.
Seriola dumerili
) has attracted considerable economic interest since the 1990s because it possesses traits that distinguish it as a promising candidate in aquaculture. However, the challenges of its successful rearing, combined with the need to develop proper management practices, underline the importance of research into the genetic diversity of both wild and farmed stocks. We aimed to decipher the genetic structure of
S. dumerili
in the Eastern Mediterranean and the Northeast Atlantic using three types of genetic markers (mitochondrial control region sequences, microsatellites, and single nucleotide polymorphisms, or SNPs) to evaluate the population genetic structure of the species. The results were compared to those of previous studies of the species focusing on the Central Mediterranean and confirmed the presence of two divergent mtDNA haplogroups distributed without any detectable geographical structuring within the Mediterranean and the Atlantic. However, population analyses of the genetic structure of the species using either 10 microsatellites or a set of 1,051 SNP markers suggest that the species forms three genetically distinct groups, one in the Mediterranean and two in the Atlantic. The latter has so far not been reported in the Northeast Atlantic, and that this differentiation refers to samples from the Canary Islands is surprising. Fifteen candidate outlier SNP loci were identified in the data set, one located within the
tead1a
/TEF-1A gene, which has been associated with temperature acclimation of the medaka. This work enriches our knowledge of the genetic diversity of wild populations of the greater amberjack in the Mediterranean and the Northeast Atlantic and attempts to investigate signs of local adaptation toward a better understanding of the species distribution patterns. Future studies should be conducted to investigate the genomic regions associated with temperature acclimation in marine organisms that have to adapt to the ongoing climate change pressures, such as sea temperature rise, in order to survive.
Fakriadis, Ioannis; Meiri-Ashkenazi, Iris; Bracha, Chen; Rosenfeld, Hanna; Corriero, Aldo; Zupa, Rosa; Pousis, Chrysovalentinos; Papadaki, Maria; Mylonas, Constantinos C
Gonadotropin expression, pituitary and plasma levels in the reproductive cycle of wild and captive-reared greater amberjack (Seriola dumerili) Journal Article
In: General and Comparative Endocrinology, vol. 350, pp. 114465, 2024, ISSN: 00166480.
@article{fakriadis_gonadotropin_2024,
title = {Gonadotropin expression, pituitary and plasma levels in the reproductive cycle of wild and captive-reared greater amberjack (Seriola dumerili)},
author = {Ioannis Fakriadis and Iris Meiri-Ashkenazi and Chen Bracha and Hanna Rosenfeld and Aldo Corriero and Rosa Zupa and Chrysovalentinos Pousis and Maria Papadaki and Constantinos C Mylonas},
url = {https://imbbc.hcmr.gr/wp-content/uploads/2024/03/2024-Fakriadis-GCE-12.pdf
https://linkinghub.elsevier.com/retrieve/pii/S001664802400025X},
doi = {10.1016/j.ygcen.2024.114465},
issn = {00166480},
year = {2024},
date = {2024-03-05},
urldate = {2024-03-05},
journal = {General and Comparative Endocrinology},
volume = {350},
pages = {114465},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Ventriglia, G; Fakriadis, I; Papadaki, M; Zupa, R; Pousis, C; Mandalakis, M; Corriero, A; Mylonas, C C
Effects of different hormonal treatments on spermatogenesis advancement in hatchery-produced greater amberjack Seriola dumerili (Risso 1810) Journal Article
In: vol. 348, pp. 114447, 2024, ISSN: 00166480.
@article{ventriglia_effects_2024,
title = {Effects of different hormonal treatments on spermatogenesis advancement in hatchery-produced greater amberjack Seriola dumerili (Risso 1810)},
author = {G Ventriglia and I Fakriadis and M Papadaki and R Zupa and C Pousis and M Mandalakis and A Corriero and C C Mylonas},
url = {https://imbbc.hcmr.gr/wp-content/uploads/2024/02/2024-Ventriglia-GCE-7.pdf
https://linkinghub.elsevier.com/retrieve/pii/S0016648024000078},
doi = {10.1016/j.ygcen.2024.114447},
issn = {00166480},
year = {2024},
date = {2024-02-05},
urldate = {2024-02-09},
volume = {348},
pages = {114447},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Lancerotto, Stefano; Fakriadis, Ioannis; Papadaki, Maria; Mandalakis, Manolis; Sigelaki, Irini; Mylonas, Constantinos C
Timing of puberty in F1-generation hatchery-produced greater amberjack (Seriola dumerili) Journal Article
In: General and Comparative Endocrinology, vol. 347, pp. 114414, 2024, ISSN: 00166480.
@article{lancerotto_timing_2024,
title = {Timing of puberty in F1-generation hatchery-produced greater amberjack (Seriola dumerili)},
author = {Stefano Lancerotto and Ioannis Fakriadis and Maria Papadaki and Manolis Mandalakis and Irini Sigelaki and Constantinos C Mylonas},
url = {https://imbbc.hcmr.gr/wp-content/uploads/2024/01/2024-Lancerotto-GCE-SI-12ISRPF-1.pdf
https://linkinghub.elsevier.com/retrieve/pii/S0016648023002198},
doi = {10.1016/j.ygcen.2023.114414},
issn = {00166480},
year = {2024},
date = {2024-01-01},
urldate = {2024-01-10},
journal = {General and Comparative Endocrinology},
volume = {347},
pages = {114414},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
2021
Fakriadis, Ioannis; Mylonas, Constantinos C
In: Fish Physiology and Biochemistry, vol. 47, no. 2, pp. 281–292, 2021, ISSN: 1573-5168.
@article{fakriadis_sperm_2021,
title = {Sperm quality of greater amberjack Seriola dumerili throughout the reproductive season and in response to GnRHa treatment with controlled release implants},
author = {Ioannis Fakriadis and Constantinos C Mylonas},
url = {https://doi.org/10.1007/s10695-020-00910-9},
doi = {10.1007/s10695-020-00910-9},
issn = {1573-5168},
year = {2021},
date = {2021-01-01},
urldate = {2021-04-27},
journal = {Fish Physiology and Biochemistry},
volume = {47},
number = {2},
pages = {281--292},
abstract = {A wild-caught stock of greater amberjack Seriola dumerili reared in sea cages in two commercial aquaculture facilities in Greece was administered gonadotropin-releasing hormone agonist (GnRHa), loaded in controlled release implants, and was used in spawning induction experiments throughout the reproductive season. Sperm quality was evaluated using computer-assisted sperm analysis (CASA) before and after GnRHa administration and the extent of the spermiation period was determined. Males were in spermiation throughout the monitoring period from May 30 until July 18, at temperatures between 19 and 24 °C. However, lower sperm motility duration, density, and survival under cold storage were observed from early July onwards. Sperm quality did not recover after the end of spawning induction experiments in tanks and the return of the fish for 14–28 days to the sea cage in mid-July, which could be related to the high temperatures of this period. An improvement trend was observed in the quality of the milt collected on day 7 after a single GnRHa administration, but a significant decrease was observed on day 21 in sperm density, survival under cold storage, and straight line velocity (VSL). On the contrary, a double GnRHa administration spaced 14 days apart maintained the same sperm quality for a longer period of 29 days. Further spermiation enhancement experiments should be conducted in the future to describe in more detail the kinetics of sperm production after GnRHa therapy and its effects on sperm quality.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
A wild-caught stock of greater amberjack Seriola dumerili reared in sea cages in two commercial aquaculture facilities in Greece was administered gonadotropin-releasing hormone agonist (GnRHa), loaded in controlled release implants, and was used in spawning induction experiments throughout the reproductive season. Sperm quality was evaluated using computer-assisted sperm analysis (CASA) before and after GnRHa administration and the extent of the spermiation period was determined. Males were in spermiation throughout the monitoring period from May 30 until July 18, at temperatures between 19 and 24 °C. However, lower sperm motility duration, density, and survival under cold storage were observed from early July onwards. Sperm quality did not recover after the end of spawning induction experiments in tanks and the return of the fish for 14–28 days to the sea cage in mid-July, which could be related to the high temperatures of this period. An improvement trend was observed in the quality of the milt collected on day 7 after a single GnRHa administration, but a significant decrease was observed on day 21 in sperm density, survival under cold storage, and straight line velocity (VSL). On the contrary, a double GnRHa administration spaced 14 days apart maintained the same sperm quality for a longer period of 29 days. Further spermiation enhancement experiments should be conducted in the future to describe in more detail the kinetics of sperm production after GnRHa therapy and its effects on sperm quality.
Mello, Paulo De H; Lancerotto, Stefano; Fakriadis, Ioannis; Tsoukali, Panagiota; Papadaki, Maria; Mylonas, Constantinos Chrysanthos
In: Mediterranean Marine Science, vol. 22, no. 2, pp. 218–231, 2021, ISSN: 1791-6763.
@article{mello_importance_2021,
title = {The importance of thermoperiod for proper gametogenesis and successful egg and sperm production in meagre (Argyrosomus regius) breeders in aquaculture},
author = {Paulo De H Mello and Stefano Lancerotto and Ioannis Fakriadis and Panagiota Tsoukali and Maria Papadaki and Constantinos Chrysanthos Mylonas},
url = {https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/25806
https://imbbc.hcmr.gr/wp-content/uploads/2021/05/2021-De-Mello-MMS-27-2.pdf},
doi = {10.12681/mms.25806},
issn = {1791-6763},
year = {2021},
date = {2021-01-01},
urldate = {2021-04-27},
journal = {Mediterranean Marine Science},
volume = {22},
number = {2},
pages = {218--231},
abstract = {We examined the effect of constant water temperature throughout the year on gametogenesis, spawning success and egg/sperm/embryo quality in meagre (Argyrosomus regius). Two broodstocks were exposed to simulated natural photoperiod, andeither attenuated seasonal water temperature (SeasT, 16.4 to 19.6ºC) or relatively constant water temperature (CoT, 19.4 ± 0.6ºC).In the spawning period (May), 4 couples per group were induced to spawn with gonadotropin releasing hormone agonist (GnRHa). Gonadal stage of development, sperm quality parameters and plasma levels of sex steroids were evaluated prior to the GnRHa treatment. Spawning success and egg/sperm quality were examined over the following 4 weeks. Constant temperature did not prevent gametogenesis, but exposure to attenuated seasonal water temperature with the inclusion of winter low temperature was beneficial to both sexes. The mean (±SD) diameter of the largest vitellogenic oocytes prior to GnRHa administration was significantly higher in the SeasT compared to the CoT group (598 ± 27 vs 520 ± 17 μm). Testosterone plasma levels in the females were significantly higher in the SeasT group, but all other hormones were similar in both sexes. SeasT females spawned more consistently with higher relative fecundity, and 24-h embryo survival of the produced eggs. A more pronounced negative effect of constant water temperature was observed in males, since CoT males exhibited a spermiation index of 0 prior to GnRHa treatment, the latter clearly having a beneficial effect over the following 4 weeks. The study demonstrated that meagre do undergo gametogenesis to a significant extent even under constant water temperatures during the year. However, a seasonal thermal regime -even an attenuated one- was necessary for the proper development of the gametes, allowing for the successful spawning induction using the established GnRHa induction protocol.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
We examined the effect of constant water temperature throughout the year on gametogenesis, spawning success and egg/sperm/embryo quality in meagre (Argyrosomus regius). Two broodstocks were exposed to simulated natural photoperiod, andeither attenuated seasonal water temperature (SeasT, 16.4 to 19.6ºC) or relatively constant water temperature (CoT, 19.4 ± 0.6ºC).In the spawning period (May), 4 couples per group were induced to spawn with gonadotropin releasing hormone agonist (GnRHa). Gonadal stage of development, sperm quality parameters and plasma levels of sex steroids were evaluated prior to the GnRHa treatment. Spawning success and egg/sperm quality were examined over the following 4 weeks. Constant temperature did not prevent gametogenesis, but exposure to attenuated seasonal water temperature with the inclusion of winter low temperature was beneficial to both sexes. The mean (±SD) diameter of the largest vitellogenic oocytes prior to GnRHa administration was significantly higher in the SeasT compared to the CoT group (598 ± 27 vs 520 ± 17 μm). Testosterone plasma levels in the females were significantly higher in the SeasT group, but all other hormones were similar in both sexes. SeasT females spawned more consistently with higher relative fecundity, and 24-h embryo survival of the produced eggs. A more pronounced negative effect of constant water temperature was observed in males, since CoT males exhibited a spermiation index of 0 prior to GnRHa treatment, the latter clearly having a beneficial effect over the following 4 weeks. The study demonstrated that meagre do undergo gametogenesis to a significant extent even under constant water temperatures during the year. However, a seasonal thermal regime -even an attenuated one- was necessary for the proper development of the gametes, allowing for the successful spawning induction using the established GnRHa induction protocol.
Superio, Joshua; Fakriadis, Ioannis; Tsigenopoulos, Costas S; Lancerotto, Stefano Adam; Rodriguez, Andrea Villena; Vervelakis, Emanuele; Mylonas, Constantinos C
Spawning kinetics and parentage contribution of European sea bass (Dicentrarchus labrax) broodstocks, and influence of GnRHa-induced spawning Journal Article
In: Aquaculture Reports, vol. 21, pp. 100766, 2021, ISSN: 23525134.
@article{superio_spawning_2021,
title = {Spawning kinetics and parentage contribution of European sea bass (Dicentrarchus labrax) broodstocks, and influence of GnRHa-induced spawning},
author = {Joshua Superio and Ioannis Fakriadis and Costas S Tsigenopoulos and Stefano Adam Lancerotto and Andrea Villena Rodriguez and Emanuele Vervelakis and Constantinos C Mylonas},
url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/07/2021-Superio-AquaRep-47.pdf
https://linkinghub.elsevier.com/retrieve/pii/S2352513421001824},
doi = {10.1016/j.aqrep.2021.100766},
issn = {23525134},
year = {2021},
date = {2021-01-01},
urldate = {2021-07-26},
journal = {Aquaculture Reports},
volume = {21},
pages = {100766},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
2020
Fakriadis, Ioannis; Zanatta, Eugenio Maria; Fleck, Renata Pontes Dos Santos; Mateo, Daybet Lorena Sena; Papadaki, Maria; Mylonas, Constantinos C
Endocrine regulation of long-term enhancement of spermiation in meagre (Argyrosomus regius) with GnRHa controlled-delivery systems Journal Article
In: General and Comparative Endocrinology, vol. 297, pp. 113549, 2020, ISSN: 00166480.
@article{fakriadis_endocrine_2020,
title = {Endocrine regulation of long-term enhancement of spermiation in meagre (Argyrosomus regius) with GnRHa controlled-delivery systems},
author = {Ioannis Fakriadis and Eugenio Maria Zanatta and Renata Pontes Dos Santos Fleck and Daybet Lorena Sena Mateo and Maria Papadaki and Constantinos C Mylonas},
url = {https://linkinghub.elsevier.com/retrieve/pii/S0016648020303026},
doi = {10.1016/j.ygcen.2020.113549},
issn = {00166480},
year = {2020},
date = {2020-10-01},
urldate = {2020-08-06},
journal = {General and Comparative Endocrinology},
volume = {297},
pages = {113549},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Fakriadis, Ioannis; Miccoli, Andrea; Karapanagiotis, Stelios; Tsele, Nikoleta; Mylonas, Constantinos C
In: Aquaculture, vol. 521, 2020, ISSN: 00448486, (Type: Journal Article).
@article{fakriadis_optimization_2020,
title = {Optimization of a GnRHa treatment for spawning commercially reared greater amberjack Seriola dumerili: Dose response and extent of the reproductive season},
author = {Ioannis Fakriadis and Andrea Miccoli and Stelios Karapanagiotis and Nikoleta Tsele and Constantinos C Mylonas},
doi = {10.1016/j.aquaculture.2020.735011},
issn = {00448486},
year = {2020},
date = {2020-01-01},
journal = {Aquaculture},
volume = {521},
note = {Type: Journal Article},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Fakriadis, Ioannis; Sigelaki, Irini; Papadaki, Maria; Papandroulakis, Nikos; Raftopoulos, Anastasios; Tsakoniti, Kalliopi; Mylonas, Constantinos C
Control of reproduction of greater amberjack Seriola dumerili reared in aquaculture facilities Journal Article
In: Aquaculture, vol. 519, 2020, ISSN: 00448486, (Type: Journal Article).
@article{fakriadis_control_2020,
title = {Control of reproduction of greater amberjack Seriola dumerili reared in aquaculture facilities},
author = {Ioannis Fakriadis and Irini Sigelaki and Maria Papadaki and Nikos Papandroulakis and Anastasios Raftopoulos and Kalliopi Tsakoniti and Constantinos C Mylonas},
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doi = {10.1016/j.aquaculture.2019.734880},
issn = {00448486},
year = {2020},
date = {2020-01-01},
journal = {Aquaculture},
volume = {519},
note = {Type: Journal Article},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
2019
Fakriadis, I; Lisi, F; Sigelaki, I; Papadaki, M; Mylonas, C C
Spawning kinetics and egg/larval quality of greater amberjack (Seriola dumerili) in response to multiple GnRHa injections or implants Journal Article
In: General and Comparative Endocrinology, vol. 279, pp. 78–87, 2019, ISSN: 1095-6840 (Electronic) 0016-6480 (Linking), (Type: Journal Article).
@article{fakriadis_spawning_2019,
title = {Spawning kinetics and egg/larval quality of greater amberjack (Seriola dumerili) in response to multiple GnRHa injections or implants},
author = {I Fakriadis and F Lisi and I Sigelaki and M Papadaki and C C Mylonas},
url = {https://www.ncbi.nlm.nih.gov/pubmed/30571964 https://www.sciencedirect.com/science/article/pii/S0016648018304726?via%3Dihub},
doi = {10.1016/j.ygcen.2018.12.007},
issn = {1095-6840 (Electronic) 0016-6480 (Linking)},
year = {2019},
date = {2019-01-01},
journal = {General and Comparative Endocrinology},
volume = {279},
pages = {78--87},
note = {Type: Journal Article},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Pérez, Evaristo; Linares, Fátima; Villanueva, José Luis Rodríguez; Vilar, Antonio; Mylonas, Constantinos C; Fakriadis, Ioannis; Papadaki, Maria; Papandroulakis, Nikos; Papadakis, Ioannis; Robles, Rocío; Fauvel, Christian; Roo, Javier; Peleteiro, José Benito; Lluch, Nuria; Pazos, Gema; Méndez, Belén; Sigelaki, Irini; Gómez, Castora; Pérez, Montse; Álvarez-Blázquez, Blanca
Wreckfish (Polyprion americanus). New Knowledge About Reproduction, Larval Husbandry, and Nutrition. Promise as a New Species for Aquaculture Journal Article
In: Fishes, vol. 4, no. 1, pp. 14, 2019, (Type: Journal Article).
@article{perez_wreckfish_2019,
title = {Wreckfish (Polyprion americanus). New Knowledge About Reproduction, Larval Husbandry, and Nutrition. Promise as a New Species for Aquaculture},
author = {Evaristo Pérez and Fátima Linares and José Luis Rodríguez Villanueva and Antonio Vilar and Constantinos C Mylonas and Ioannis Fakriadis and Maria Papadaki and Nikos Papandroulakis and Ioannis Papadakis and Rocío Robles and Christian Fauvel and Javier Roo and José Benito Peleteiro and Nuria Lluch and Gema Pazos and Belén Méndez and Irini Sigelaki and Castora Gómez and Montse Pérez and Blanca Álvarez-Blázquez},
year = {2019},
date = {2019-01-01},
journal = {Fishes},
volume = {4},
number = {1},
pages = {14},
note = {Type: Journal Article},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Forner-Piquer, Isabel; Fakriadis, Ioannis; Mylonas, Constantinos C; Piscitelli, Fabiana; Marzo, Vincenzo Di; Maradonna, Francesca; Calduch-Giner, Josep; Pérez-Sánchez, Jaume; Carnevali, Oliana
Effects of Dietary Bisphenol A on the Reproductive Function of Gilthead Sea Bream (Sparus aurata) Testes Journal Article
In: International Journal of Molecular Sciences, vol. 20, no. 20, pp. 5003, 2019, (Type: Journal Article).
@article{forner-piquer_effects_2019,
title = {Effects of Dietary Bisphenol A on the Reproductive Function of Gilthead Sea Bream (Sparus aurata) Testes},
author = {Isabel Forner-Piquer and Ioannis Fakriadis and Constantinos C Mylonas and Fabiana Piscitelli and Vincenzo Di Marzo and Francesca Maradonna and Josep Calduch-Giner and Jaume Pérez-Sánchez and Oliana Carnevali},
url = {https://dx.doi.org/10.3390%2Fijms20205003},
doi = {10.3390/ijms20205003},
year = {2019},
date = {2019-01-01},
journal = {International Journal of Molecular Sciences},
volume = {20},
number = {20},
pages = {5003},
note = {Type: Journal Article},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Forner-Piquer, Isabel; Mylonas, Constantinos C; Fakriadis, Ioannis; Papadaki, Maria; Piscitelli, Fabiana; Marzo, Vincenzo Di; Calduch-Giner, Josep; Pérez-Sánchez, Jaume; Carnevali, Oliana
In: Archives of Toxicology, 2019, ISSN: 1432-0738, (Type: Journal Article).
@article{forner-piquer_effects_2019-1,
title = {Effects of diisononyl phthalate (DiNP) on the endocannabinoid and reproductive systems of male gilthead sea bream (Sparus aurata) during the spawning season},
author = {Isabel Forner-Piquer and Constantinos C Mylonas and Ioannis Fakriadis and Maria Papadaki and Fabiana Piscitelli and Vincenzo Di Marzo and Josep Calduch-Giner and Jaume Pérez-Sánchez and Oliana Carnevali},
url = {https://doi.org/10.1007/s00204-018-2378-6},
doi = {10.1007/s00204-018-2378-6},
issn = {1432-0738},
year = {2019},
date = {2019-01-01},
journal = {Archives of Toxicology},
note = {Type: Journal Article},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
2018
Pousis, C; Mylonas, C C; Virgilio, C De; Gadaleta, G; Santamaria, N; Passantino, L; Zupa, R; Papadaki, M; Fakriadis, I; Ferreri, R; Corriero, A
In: Aquaculture Research, vol. 49, no. 1, pp. 243–252, 2018, ISSN: 1355557X, (Publisher: Blackwell Publishing Ltd).
@article{pousis_observed_2018,
title = {The observed oogenesis impairment in greater amberjack Seriola dumerili (Risso, 1810) reared in captivity is not related to an insufficient liver transcription or oocyte uptake of vitellogenin},
author = {C Pousis and C C Mylonas and C De Virgilio and G Gadaleta and N Santamaria and L Passantino and R Zupa and M Papadaki and I Fakriadis and R Ferreri and A Corriero},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85026499054&doi=10.1111%2fare.13453&partnerID=40&md5=ecb8f6c6b9bbbf8b803322299447cfd4},
doi = {10.1111/are.13453},
issn = {1355557X},
year = {2018},
date = {2018-01-01},
journal = {Aquaculture Research},
volume = {49},
number = {1},
pages = {243--252},
abstract = {The greater amberjack Seriola dumerili is an excellent candidate for the Mediterranean aquaculture, due to its large body size and high growth rate, as well as its high flesh quality and commercial value worldwide. For its successful incorporation in the aquaculture industry, an in-depth understanding of the reproductive function of the species under rearing conditions is necessary, since completion of oogenesis in captivity is currently a bottleneck for the commercial production of the species. Liver and ovary samples from wild and captive-reared greater amberjack females were collected at three different phases of the reproductive cycle: early gametogenesis (EARLY, late April-early May), advanced gametogenesis (ADVANCED, late May-early June) and spawning (SPAWNING, late June-July). The cDNAs of three vitellogenins (VtgA, VtgB and VtgC) were partially sequenced and a qRT-PCR for their expression was used to compare ovarian maturity stage and liver vitellogenin transcript levels between wild and captive-reared individuals. An extensive atresia of late vitellogenic follicles, which prevented any further oocyte development and spawning was observed in captive-reared individuals during the ADVANCED phase. The expression levels of the three vitellogenins, as well as the amount of yolk globules in vitellogenic oocytes, did not differ significantly between captive-reared and wild females, indicating that the observed oogenesis impairment in greater amberjack reared in captivity was not related to an insufficient liver synthesis or a reduced oocyte uptake of vitellogenin. © 2017 John Wiley & Sons Ltd},
note = {Publisher: Blackwell Publishing Ltd},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The greater amberjack Seriola dumerili is an excellent candidate for the Mediterranean aquaculture, due to its large body size and high growth rate, as well as its high flesh quality and commercial value worldwide. For its successful incorporation in the aquaculture industry, an in-depth understanding of the reproductive function of the species under rearing conditions is necessary, since completion of oogenesis in captivity is currently a bottleneck for the commercial production of the species. Liver and ovary samples from wild and captive-reared greater amberjack females were collected at three different phases of the reproductive cycle: early gametogenesis (EARLY, late April-early May), advanced gametogenesis (ADVANCED, late May-early June) and spawning (SPAWNING, late June-July). The cDNAs of three vitellogenins (VtgA, VtgB and VtgC) were partially sequenced and a qRT-PCR for their expression was used to compare ovarian maturity stage and liver vitellogenin transcript levels between wild and captive-reared individuals. An extensive atresia of late vitellogenic follicles, which prevented any further oocyte development and spawning was observed in captive-reared individuals during the ADVANCED phase. The expression levels of the three vitellogenins, as well as the amount of yolk globules in vitellogenic oocytes, did not differ significantly between captive-reared and wild females, indicating that the observed oogenesis impairment in greater amberjack reared in captivity was not related to an insufficient liver synthesis or a reduced oocyte uptake of vitellogenin. © 2017 John Wiley & Sons Ltd
Papadaki, M; Mazzella, D; Santinelli, V; Fakriadis, I; Sigelaki, I; Mylonas, C C
Hermaphroditism and reproductive function of hatchery-produced sharpsnout seabream (Diplodus puntazzo) under attenuated annual thermal cycles Journal Article
In: Aquaculture, vol. 482, pp. 231–240, 2018, ISSN: 00448486, (Publisher: Elsevier B.V.).
@article{papadaki_hermaphroditism_2018,
title = {Hermaphroditism and reproductive function of hatchery-produced sharpsnout seabream (Diplodus puntazzo) under attenuated annual thermal cycles},
author = {M Papadaki and D Mazzella and V Santinelli and I Fakriadis and I Sigelaki and C C Mylonas},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85030865182&doi=10.1016%2fj.aquaculture.2017.09.028&partnerID=40&md5=61ad82357d509cb8f19491299ca570e0},
doi = {10.1016/j.aquaculture.2017.09.028},
issn = {00448486},
year = {2018},
date = {2018-01-01},
journal = {Aquaculture},
volume = {482},
pages = {231--240},
abstract = {Acquiring more knowledge on the reproductive biology of the sharpsnout seabream (Diplodus puntazzo) can improve our ability to control its reproduction and enable hatcheries to reconsider this fish as a potential species for the Mediterranean aquaculture production. We investigated hermaphroditism and reproductive function in a hatchery-produced broodstock over a long period of 6 years, which includes sex differentiation, puberty and four consecutive annual reproductive seasons, at relatively constant water temperatures of 18–20 °C, which are typical of borehole seawater supplies used frequently in marine hatcheries for biosecurity reasons. At the end of the first year of life, the gonads were bisexual and were classified into three different categories: mainly testicular tissue with rudimentary ovarian tissue (Mf), substantial amounts of both tissues (MF) and mainly ovarian tissue with rudimentary testicular tissue (mF). Fully female (F) gonads appeared already in 1 + years old fish, whereas fully male (M) gonads appeared for the first time in 2 + years old fish. The sex ratio (F:M) of the studied populations during the first 6 years of life in all age classes was not different from 1:1. Therefore, no annual restructuring of the broodstock is necessary in order to avoid the development of skewed sex ratios, since once functional sex is established it does not seem to change in the following years. Furthermore, the use of attenuated annual thermal cycling regimes did not seem to affect the annual gametogenic cycle and full maturation of this species, though spontaneous spawning was still missing and the fish exhibited the typical problems reported for this species in captivity. © 2017 Elsevier B.V.},
note = {Publisher: Elsevier B.V.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Acquiring more knowledge on the reproductive biology of the sharpsnout seabream (Diplodus puntazzo) can improve our ability to control its reproduction and enable hatcheries to reconsider this fish as a potential species for the Mediterranean aquaculture production. We investigated hermaphroditism and reproductive function in a hatchery-produced broodstock over a long period of 6 years, which includes sex differentiation, puberty and four consecutive annual reproductive seasons, at relatively constant water temperatures of 18–20 °C, which are typical of borehole seawater supplies used frequently in marine hatcheries for biosecurity reasons. At the end of the first year of life, the gonads were bisexual and were classified into three different categories: mainly testicular tissue with rudimentary ovarian tissue (Mf), substantial amounts of both tissues (MF) and mainly ovarian tissue with rudimentary testicular tissue (mF). Fully female (F) gonads appeared already in 1 + years old fish, whereas fully male (M) gonads appeared for the first time in 2 + years old fish. The sex ratio (F:M) of the studied populations during the first 6 years of life in all age classes was not different from 1:1. Therefore, no annual restructuring of the broodstock is necessary in order to avoid the development of skewed sex ratios, since once functional sex is established it does not seem to change in the following years. Furthermore, the use of attenuated annual thermal cycling regimes did not seem to affect the annual gametogenic cycle and full maturation of this species, though spontaneous spawning was still missing and the fish exhibited the typical problems reported for this species in captivity. © 2017 Elsevier B.V.
Jerez, Salvador; Fakriadis, Ioannis; Papadaki, Maria; Martín, M; Cejas, Juana; Mylonas, Constantinos C
Spawning induction of first-generation (F1) greater amberjack Seriola dumerili in the Canary Islands, Spain using GnRHa delivery systems Journal Article
In: Fishes, vol. 3, no. 3, pp. 1–22, 2018, ISSN: 2410-3888, (Type: Journal Article).
@article{jerez_spawning_2018,
title = {Spawning induction of first-generation (F1) greater amberjack Seriola dumerili in the Canary Islands, Spain using GnRHa delivery systems},
author = {Salvador Jerez and Ioannis Fakriadis and Maria Papadaki and M Martín and Juana Cejas and Constantinos C Mylonas},
url = {https://res.mdpi.com/fishes/fishes-03-00035/article_deploy/fishes-03-00035.pdf?filename=&attachment=1},
doi = {10.3390/fishes3030035},
issn = {2410-3888},
year = {2018},
date = {2018-01-01},
journal = {Fishes},
volume = {3},
number = {3},
pages = {1--22},
note = {Type: Journal Article},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Papadaki, Maria; Peleteiro, José Benito; Alvarez-Blázquez, Blanca; Villanueva, José Luis Rodríguez; Linares, Fatima; Vilar, Antonio; Rial, Evaristo Pérez; Lluch, Nuria; Fakriadis, Ioannis; Sigelaki, Irini; Mylonas, Constantinos C
Description of the Annual Reproductive Cycle of Wreckfish Polyprion americanus in Captivity Journal Article
In: Fishes, vol. 3, no. 4, pp. 1–20, 2018, ISSN: 2410-3888, (Type: Journal Article).
@article{papadaki_description_2018,
title = {Description of the Annual Reproductive Cycle of Wreckfish Polyprion americanus in Captivity},
author = {Maria Papadaki and José Benito Peleteiro and Blanca Alvarez-Blázquez and José Luis Rodríguez Villanueva and Fatima Linares and Antonio Vilar and Evaristo Pérez Rial and Nuria Lluch and Ioannis Fakriadis and Irini Sigelaki and Constantinos C Mylonas},
url = {https://res.mdpi.com/fishes/fishes-03-00043/article_deploy/fishes-03-00043.pdf?filename=&attachment=1},
doi = {10.3390/fishes3040043},
issn = {2410-3888},
year = {2018},
date = {2018-01-01},
journal = {Fishes},
volume = {3},
number = {4},
pages = {1--20},
note = {Type: Journal Article},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
2017
Zupa, R; Rodrõâguez, C; Mylonas, C C; Rosenfeld, H; Fakriadis, I; Papadaki, M; Peârez, J A; Pousis, C; Basilone, G; Corriero, A
Comparative study of reproductive development in wild and captive-reared greater amberjack seriola dumerili (Risso, 1810) Journal Article
In: PLoS ONE, vol. 12, no. 1, 2017, ISSN: 19326203, (Publisher: Public Library of Science).
@article{zupa_comparative_2017,
title = {Comparative study of reproductive development in wild and captive-reared greater amberjack seriola dumerili (Risso, 1810)},
author = {R Zupa and C Rodrõâguez and C C Mylonas and H Rosenfeld and I Fakriadis and M Papadaki and J A Peârez and C Pousis and G Basilone and A Corriero},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85008686824&doi=10.1371%2fjournal.pone.0169645&partnerID=40&md5=09301e14b368a835887bf0e7176db149},
doi = {10.1371/journal.pone.0169645},
issn = {19326203},
year = {2017},
date = {2017-01-01},
journal = {PLoS ONE},
volume = {12},
number = {1},
abstract = {The greater amberjack Seriola dumerili is a large teleost fish with rapid growth and excellent flesh quality, whose domestication represents an ambitious challenge for aquaculture. The occurrence of reproductive dysfunctions in greater amberjack reared in captivity was investigated by comparing reproductive development of wild and captive-reared individuals. Wild and captive-reared breeders were sampled in the Mediterranean Sea during three different phases of the reproductive cycle: Early gametogenesis (EARLY, late April-early May), advanced gametogenesis (ADVANCED, late May-early June) and spawning (SPAWNING, late June-July). Fish reproductive state was evaluated using the gonado-somatic index (GSI), histological analysis of the gonads and determination of sex steroid levels in the plasma, and correlated with leptin expression in the liver and gonad biochemical composition. The GSI and sex steroid levels were lower in captive-reared than in wild fish. During the ADVANCED period, when the wild greater amberjack breeders were already in spawning condition, ovaries of captive-reared breeders showed extensive atresia of late vitellogenic oocytes and spermatogenic activity ceased in the testes of half of the examined males. During the SPAWNING period, all captive-reared fish had regressed gonads, while wild breeders still displayed reproductive activity. Liver leptin expression and gonad proximate composition of wild and captive greater amberjack were similar. However, the gonads of captive-reared fish showed different total polar lipid contents, as well as specific lipid classes and fatty acid profiles with respect to wild individuals. This study underlines the need for an improvement in rearing technology for this species, which should include minimum handling during the reproductive season and the formulation of a specific diet to overcome the observed gonadal decrements of phospholipids, DHA (22:6n-3) and ARA (20:4n-6), compared to wild breeders. © 2017 Zupa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},
note = {Publisher: Public Library of Science},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The greater amberjack Seriola dumerili is a large teleost fish with rapid growth and excellent flesh quality, whose domestication represents an ambitious challenge for aquaculture. The occurrence of reproductive dysfunctions in greater amberjack reared in captivity was investigated by comparing reproductive development of wild and captive-reared individuals. Wild and captive-reared breeders were sampled in the Mediterranean Sea during three different phases of the reproductive cycle: Early gametogenesis (EARLY, late April-early May), advanced gametogenesis (ADVANCED, late May-early June) and spawning (SPAWNING, late June-July). Fish reproductive state was evaluated using the gonado-somatic index (GSI), histological analysis of the gonads and determination of sex steroid levels in the plasma, and correlated with leptin expression in the liver and gonad biochemical composition. The GSI and sex steroid levels were lower in captive-reared than in wild fish. During the ADVANCED period, when the wild greater amberjack breeders were already in spawning condition, ovaries of captive-reared breeders showed extensive atresia of late vitellogenic oocytes and spermatogenic activity ceased in the testes of half of the examined males. During the SPAWNING period, all captive-reared fish had regressed gonads, while wild breeders still displayed reproductive activity. Liver leptin expression and gonad proximate composition of wild and captive greater amberjack were similar. However, the gonads of captive-reared fish showed different total polar lipid contents, as well as specific lipid classes and fatty acid profiles with respect to wild individuals. This study underlines the need for an improvement in rearing technology for this species, which should include minimum handling during the reproductive season and the formulation of a specific diet to overcome the observed gonadal decrements of phospholipids, DHA (22:6n-3) and ARA (20:4n-6), compared to wild breeders. © 2017 Zupa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Zupa, R; Fauvel, C; Mylonas, C C; Pousis, C; Santamaria, N; Papadaki, Μ; Fakriadis, I; Cicirelli, V; Mangano, S; Passantino, L; Lacalandra, G M; Corriero, A
Rearing in captivity affects spermatogenesis and sperm quality in greater amberjack, Seriola dumerili (Risso, 1810) Journal Article
In: Journal of Animal Science, vol. 95, no. 9, pp. 4085–4100, 2017, ISSN: 00218812, (Publisher: American Society of Animal Science).
@article{zupa_rearing_2017,
title = {Rearing in captivity affects spermatogenesis and sperm quality in greater amberjack, Seriola dumerili (Risso, 1810)},
author = {R Zupa and C Fauvel and C C Mylonas and C Pousis and N Santamaria and Μ Papadaki and I Fakriadis and V Cicirelli and S Mangano and L Passantino and G M Lacalandra and A Corriero},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85029717001&doi=10.2527%2fjas.2017.1708&partnerID=40&md5=93369a73080df336a351b6a9a006cab1},
doi = {10.2527/jas.2017.1708},
issn = {00218812},
year = {2017},
date = {2017-01-01},
journal = {Journal of Animal Science},
volume = {95},
number = {9},
pages = {4085--4100},
abstract = {The greater amberjack, Seriola dumerili (Risso, 1810), is a promising candidate for the diversification of European aquaculture production, but inconsistent reproduction in captivity prevents commercial production. Recent studies showed that greater amberjack confined in sea cages exhibited scarce gonad development and early interruption of gametogenic activity during the reproductive season. The aim of the present study was to improve our understanding of the observed impairment of spermatogenesis. Adult wild and captive-reared males were sampled during 3 different phases of the reproductive cycle: early gametogenesis (EARLY; late April to early May), advanced gametogenesis (ADVANCED; late May to early June), and spawning (SPAWNING; late June to July). Spermatogonial stem cells and proliferating germ cells were identified through the immunohistochemical localization of Pou5f1 and proliferating cell nuclear antigen, respectively. Apoptotic germ cells were identified throughout the terminal deoxynucleotidyl transferase-mediated 2’-deoxyuridine 5’-triphosphate nick end labeling method. Sperm quality of captive-reared fish was evaluated using computer-assisted sperm analysis. Captive-reared males exhibited seminiferous lobules of a smaller diameter, a precocious and progressive decrease of spermatogonial mitosis, and a high level of apoptosis at the beginning of the reproductive season, concomitant with a many-fold higher 17β-estradiol plasma concentration. The motile spermatozoa percentage of captive greater amberjack was lower than in other teleosts, and a drastic decrease of spermatozoa motility duration, velocity, and ATP content occurred along the reproductive season. An abnormal increase of sperm concentration as well as an increase of dead spermatozoa occurred during the SPAWNING phase, probably because of lack of sperm hydration and ejaculation and consequent sperm ageing. The present study demonstrates the extreme susceptibility of greater amberjack to rearing stress and underscores the need for improvement of the rearing and handling procedures to ameliorate gametogenesis dysfunctions in commercial aquaculture production. © 2017 American Society of Animal Science. All rights reserved.},
note = {Publisher: American Society of Animal Science},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The greater amberjack, Seriola dumerili (Risso, 1810), is a promising candidate for the diversification of European aquaculture production, but inconsistent reproduction in captivity prevents commercial production. Recent studies showed that greater amberjack confined in sea cages exhibited scarce gonad development and early interruption of gametogenic activity during the reproductive season. The aim of the present study was to improve our understanding of the observed impairment of spermatogenesis. Adult wild and captive-reared males were sampled during 3 different phases of the reproductive cycle: early gametogenesis (EARLY; late April to early May), advanced gametogenesis (ADVANCED; late May to early June), and spawning (SPAWNING; late June to July). Spermatogonial stem cells and proliferating germ cells were identified through the immunohistochemical localization of Pou5f1 and proliferating cell nuclear antigen, respectively. Apoptotic germ cells were identified throughout the terminal deoxynucleotidyl transferase-mediated 2’-deoxyuridine 5’-triphosphate nick end labeling method. Sperm quality of captive-reared fish was evaluated using computer-assisted sperm analysis. Captive-reared males exhibited seminiferous lobules of a smaller diameter, a precocious and progressive decrease of spermatogonial mitosis, and a high level of apoptosis at the beginning of the reproductive season, concomitant with a many-fold higher 17β-estradiol plasma concentration. The motile spermatozoa percentage of captive greater amberjack was lower than in other teleosts, and a drastic decrease of spermatozoa motility duration, velocity, and ATP content occurred along the reproductive season. An abnormal increase of sperm concentration as well as an increase of dead spermatozoa occurred during the SPAWNING phase, probably because of lack of sperm hydration and ejaculation and consequent sperm ageing. The present study demonstrates the extreme susceptibility of greater amberjack to rearing stress and underscores the need for improvement of the rearing and handling procedures to ameliorate gametogenesis dysfunctions in commercial aquaculture production. © 2017 American Society of Animal Science. All rights reserved.
2016
Flack, A; Fiedler, W; Blas, J; Pokrovsky, I; Kaatz, M; Mitropolsky, M; Aghababyan, K; Fakriadis, I; Makrigianni, E; Jerzak, L; Azafzaf, H; Feltrup-Azafzaf, C; Rotics, S; Mokotjomela, T M; Nathan, R; Wikelski, M
Costs of migratory decisions: A comparison across eight white stork populations Journal Article
In: Science Advances, vol. 2, no. 1, 2016, ISSN: 23752548, (Publisher: American Association for the Advancement of Science).
@article{flack_costs_2016,
title = {Costs of migratory decisions: A comparison across eight white stork populations},
author = {A Flack and W Fiedler and J Blas and I Pokrovsky and M Kaatz and M Mitropolsky and K Aghababyan and I Fakriadis and E Makrigianni and L Jerzak and H Azafzaf and C Feltrup-Azafzaf and S Rotics and T M Mokotjomela and R Nathan and M Wikelski},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84974545850&doi=10.1126%2fsciadv.1500931&partnerID=40&md5=512900f1a33f4132dce13117d925ce50},
doi = {10.1126/sciadv.1500931},
issn = {23752548},
year = {2016},
date = {2016-01-01},
journal = {Science Advances},
volume = {2},
number = {1},
abstract = {Annual migratory movements can range from a few tens to thousands of kilometers, creating unique energetic requirements for each specific species and journey. Even within the same species, migration costs can vary largely because of flexible, opportunistic life history strategies. We uncover the large extent of variation in the lifetime migratory decisions of young white storks originating from eight populations. Not only did juvenile storks differ in their geographically distinct wintering locations, their diverse migration patterns also affected the amount of energy individuals invested for locomotion during the first months of their life. Overwintering in areas with higher human population reduced the stork's overall energy expenditure because of shorter daily foraging trips, closer wintering grounds, or a complete suppression of migration. Because migrants can change ecological processes in several distinct communities simultaneously, understanding their life history decisions helps not only to protect migratory species but also to conserve stable ecosystems. © The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science.},
note = {Publisher: American Association for the Advancement of Science},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Annual migratory movements can range from a few tens to thousands of kilometers, creating unique energetic requirements for each specific species and journey. Even within the same species, migration costs can vary largely because of flexible, opportunistic life history strategies. We uncover the large extent of variation in the lifetime migratory decisions of young white storks originating from eight populations. Not only did juvenile storks differ in their geographically distinct wintering locations, their diverse migration patterns also affected the amount of energy individuals invested for locomotion during the first months of their life. Overwintering in areas with higher human population reduced the stork's overall energy expenditure because of shorter daily foraging trips, closer wintering grounds, or a complete suppression of migration. Because migrants can change ecological processes in several distinct communities simultaneously, understanding their life history decisions helps not only to protect migratory species but also to conserve stable ecosystems. © The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science.
Mylonas, C C; Salone, S; Biglino, T; de Mello, P H; Fakriadis, I; Sigelaki, I; Duncan, N
Enhancement of oogenesis/spermatogenesis in meagre Argyrosomus regius using a combination of temperature control and GnRHa treatments Journal Article
In: Aquaculture, vol. 464, pp. 323–330, 2016, ISSN: 00448486, (Publisher: Elsevier B.V.).
@article{mylonas_enhancement_2016,
title = {Enhancement of oogenesis/spermatogenesis in meagre Argyrosomus regius using a combination of temperature control and GnRHa treatments},
author = {C C Mylonas and S Salone and T Biglino and P H de Mello and I Fakriadis and I Sigelaki and N Duncan},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84978193838&doi=10.1016%2fj.aquaculture.2016.07.006&partnerID=40&md5=33e0a7321455743f1b6c84fcc8aedb38},
doi = {10.1016/j.aquaculture.2016.07.006},
issn = {00448486},
year = {2016},
date = {2016-01-01},
journal = {Aquaculture},
volume = {464},
pages = {323--330},
abstract = {A multiple GnRHa injection method for inducing spawning of high fecundity and quality in meagre was optimized. Specifically, the study (a) examined how long females would continue spawning in response to consecutive, weekly GnRHa injections, if maintained at the natural spawning temperature, and (b) evaluated whether males can produce adequate amounts of good quality sperm for the same period, with the assistance of a GnRHa therapy. Combined with stable temperatures (19–20 °C) that are prevalent during the early spawning season (April–May) in the Mediterranean Sea, each weekly GnRHa injection induced 2 consecutive spawns per week for a period of 17 weeks. Fish spawned consistently 2 days after treatment and produced high fecundity and egg quality (i.e. fertilization, hatching and early larval development). Maximum mean total fecundity (± S.D.) obtained was 1,415,000 ± 149,000 eggs kg− 1 in 32 spawns, being the highest total season fecundity reported for meagre, and significantly higher than the estimated maximum potential fecundity for the species. At the same time, sperm of consistently high quality (i.e. initial motility, duration of motility and storage survival) was produced in response to treatment with multiple GnRHa implants, though towards the end of the experiment a significant reduction (ANOVA, P ≤ 0.05) in spermatozoa density was observed. This protocol is expected to enhance greatly commercial production of meagre. Statement of relevance The present study reports on an optimized protocol for the induction of consistent and repeated spawning in meagre, based on the use of weekly GnRHa injections combined with stable temperatures that are prevalent at the early spawning season in the Mediterranean Sea. This protocol produced eggs of high fecundity and quality (i.e. fertilization, hatching and early larval development) for a period of > 4 months, and it is expected to enhance greatly commercial production of meagre. © 2016},
note = {Publisher: Elsevier B.V.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
A multiple GnRHa injection method for inducing spawning of high fecundity and quality in meagre was optimized. Specifically, the study (a) examined how long females would continue spawning in response to consecutive, weekly GnRHa injections, if maintained at the natural spawning temperature, and (b) evaluated whether males can produce adequate amounts of good quality sperm for the same period, with the assistance of a GnRHa therapy. Combined with stable temperatures (19–20 °C) that are prevalent during the early spawning season (April–May) in the Mediterranean Sea, each weekly GnRHa injection induced 2 consecutive spawns per week for a period of 17 weeks. Fish spawned consistently 2 days after treatment and produced high fecundity and egg quality (i.e. fertilization, hatching and early larval development). Maximum mean total fecundity (± S.D.) obtained was 1,415,000 ± 149,000 eggs kg− 1 in 32 spawns, being the highest total season fecundity reported for meagre, and significantly higher than the estimated maximum potential fecundity for the species. At the same time, sperm of consistently high quality (i.e. initial motility, duration of motility and storage survival) was produced in response to treatment with multiple GnRHa implants, though towards the end of the experiment a significant reduction (ANOVA, P ≤ 0.05) in spermatozoa density was observed. This protocol is expected to enhance greatly commercial production of meagre. Statement of relevance The present study reports on an optimized protocol for the induction of consistent and repeated spawning in meagre, based on the use of weekly GnRHa injections combined with stable temperatures that are prevalent at the early spawning season in the Mediterranean Sea. This protocol produced eggs of high fecundity and quality (i.e. fertilization, hatching and early larval development) for a period of > 4 months, and it is expected to enhance greatly commercial production of meagre. © 2016
2008
Peñaranda, D S; Pérez, L; Fakriadis, G; Mylonas, C C; Asturiano, J F
Effects of extenders and cryoprotectant combinations on motility and morphometry of sea bass (Dicentrarchus labrax) spermatozoa Journal Article
In: Journal of Applied Ichthyology, vol. 24, no. 4, pp. 450–455, 2008, ISSN: 01758659.
@article{penaranda_effects_2008,
title = {Effects of extenders and cryoprotectant combinations on motility and morphometry of sea bass (Dicentrarchus labrax) spermatozoa},
author = {D S Peñaranda and L Pérez and G Fakriadis and C C Mylonas and J F Asturiano},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-48249144792&doi=10.1111%2fj.1439-0426.2008.01124.x&partnerID=40&md5=4f3e81039442457697bc5340cde58c28},
doi = {10.1111/j.1439-0426.2008.01124.x},
issn = {01758659},
year = {2008},
date = {2008-01-01},
journal = {Journal of Applied Ichthyology},
volume = {24},
number = {4},
pages = {450--455},
abstract = {The aim of this study was to evaluate the effects of different extenders on sea bass (Dicentrarchus labrax) spermatozoa motility and morphology. Six sperm extenders based on inactivator media, DI1 (here named SAN) and Non-Activating Medium (NAM) were tested with European sea bass spermatozoa. The best results were obtained with NAM medium (59.83 mm NaCl, 12.91 mm MgCl 2, 1.47 mm KCl, 3.51 mm CaCl2, 20 mm NaHCO3, 0.44 mm glucose) plus 1 and 2% of BSA (NAM1 and NAM2, respectively). The motility of the spermatozoa incubated in those media was similar to the fresh sperm until 48 h (NAM1: 74.3 ± 5.4; NAM2: 78.8 ± 5.8%, and higher than undiluted sperm, 19.1 ± 7.8). We also checked the spermatozoa motility and morphology reactions with some of the best extenders, NAM2 and SAN, and combined them with different concentrations (2, 5, 10%) of three cryoprotectants: methanol, glycerol and dimethyl sulphoxide (DMSO). Glycerol + SAN or NAM2 caused activation of spermatozoa motility, which was lost 5 min later. Methanol and DMSO plus NAM2 extenders resulted in a low activation level and high motility 5 min after incubation, identifying these combinations as good candidates to be used in the cryopreservation of the European sea bass spermatozoa. © 2008 Blackwell Verlag, Berlin.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The aim of this study was to evaluate the effects of different extenders on sea bass (Dicentrarchus labrax) spermatozoa motility and morphology. Six sperm extenders based on inactivator media, DI1 (here named SAN) and Non-Activating Medium (NAM) were tested with European sea bass spermatozoa. The best results were obtained with NAM medium (59.83 mm NaCl, 12.91 mm MgCl 2, 1.47 mm KCl, 3.51 mm CaCl2, 20 mm NaHCO3, 0.44 mm glucose) plus 1 and 2% of BSA (NAM1 and NAM2, respectively). The motility of the spermatozoa incubated in those media was similar to the fresh sperm until 48 h (NAM1: 74.3 ± 5.4; NAM2: 78.8 ± 5.8%, and higher than undiluted sperm, 19.1 ± 7.8). We also checked the spermatozoa motility and morphology reactions with some of the best extenders, NAM2 and SAN, and combined them with different concentrations (2, 5, 10%) of three cryoprotectants: methanol, glycerol and dimethyl sulphoxide (DMSO). Glycerol + SAN or NAM2 caused activation of spermatozoa motility, which was lost 5 min later. Methanol and DMSO plus NAM2 extenders resulted in a low activation level and high motility 5 min after incubation, identifying these combinations as good candidates to be used in the cryopreservation of the European sea bass spermatozoa. © 2008 Blackwell Verlag, Berlin.