Ioannis Fakriadis

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2024

Superio, Joshua; Resseguier, Julien; Nobrega, Rafael Henrique; Grebstad, Caroline M; Fakriadis, Ioannis; Foss, Atle; Hagen, Ørjan; Zhang, Meiling; García-Hernández, Maria Del Pilar; Galindo-Villegas, Jorge

Unravelling spermatogenesis in spotted wolffish: Insights from the ultrastructure of juvenile male testes to the cryopreservation of broodstock sperm Journal Article

Aquaculture, 592 , pp. 741214, 2024, ISSN: 00448486.

Links | BibTeX

Papadaki, Maria; Karamanlidis, Dimitris; Sigelaki, Eirini; Fakriadis, Ioannis; Mylonas, Constantinos C

Evolution of sex ratio and egg production of gilthead seabream (Sparus aurata) over the course of five reproductive seasons Journal Article

Aquaculture and Fisheries, 9 (4), pp. 534–542, 2024, ISSN: 2468550X.

Links | BibTeX

Katirtzoglou, Aikaterini; Tsaparis, Dimitris; Kolios, Evangelos; Magoulas, Antonios; Mylonas, Constantinos C; Fakriadis, Ioannis; Manousaki, Tereza; Tsigenopoulos, Costas S

Population genomic analysis of the greater amberjack (Seriola dumerili) in the Mediterranean and the Northeast Atlantic, based on SNPs, microsatellites, and mitochondrial DNA sequences Journal Article

Frontiers in Fish Science, 2 , pp. 1356313, 2024, ISSN: 2813-9097.

Abstract | Links | BibTeX

@article{katirtzoglou_population_2024,
title = {Population genomic analysis of the greater amberjack (\textit{Seriola dumerili}) in the Mediterranean and the Northeast Atlantic, based on SNPs, microsatellites, and mitochondrial DNA sequences},
author = {Aikaterini Katirtzoglou and Dimitris Tsaparis and Evangelos Kolios and Antonios Magoulas and Constantinos C Mylonas and Ioannis Fakriadis and Tereza Manousaki and Costas S Tsigenopoulos},
url = {https://imbbc.hcmr.gr/wp-content/uploads/2024/03/2024-Kaitirtzoglou-frish-14.pdf
https://www.frontiersin.org/articles/10.3389/frish.2024.1356313/full},
doi = {10.3389/frish.2024.1356313},
issn = {2813-9097},
year = {2024},
date = {2024-03-14},
urldate = {2024-03-14},
journal = {Frontiers in Fish Science},
volume = {2},
pages = {1356313},
abstract = {The greater amberjack (
Seriola dumerili
) has attracted considerable economic interest since the 1990s because it possesses traits that distinguish it as a promising candidate in aquaculture. However, the challenges of its successful rearing, combined with the need to develop proper management practices, underline the importance of research into the genetic diversity of both wild and farmed stocks. We aimed to decipher the genetic structure of
S. dumerili
in the Eastern Mediterranean and the Northeast Atlantic using three types of genetic markers (mitochondrial control region sequences, microsatellites, and single nucleotide polymorphisms, or SNPs) to evaluate the population genetic structure of the species. The results were compared to those of previous studies of the species focusing on the Central Mediterranean and confirmed the presence of two divergent mtDNA haplogroups distributed without any detectable geographical structuring within the Mediterranean and the Atlantic. However, population analyses of the genetic structure of the species using either 10 microsatellites or a set of 1,051 SNP markers suggest that the species forms three genetically distinct groups, one in the Mediterranean and two in the Atlantic. The latter has so far not been reported in the Northeast Atlantic, and that this differentiation refers to samples from the Canary Islands is surprising. Fifteen candidate outlier SNP loci were identified in the data set, one located within the
tead1a
/TEF-1A gene, which has been associated with temperature acclimation of the medaka. This work enriches our knowledge of the genetic diversity of wild populations of the greater amberjack in the Mediterranean and the Northeast Atlantic and attempts to investigate signs of local adaptation toward a better understanding of the species distribution patterns. Future studies should be conducted to investigate the genomic regions associated with temperature acclimation in marine organisms that have to adapt to the ongoing climate change pressures, such as sea temperature rise, in order to survive.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

The greater amberjack (
Seriola dumerili
) has attracted considerable economic interest since the 1990s because it possesses traits that distinguish it as a promising candidate in aquaculture. However, the challenges of its successful rearing, combined with the need to develop proper management practices, underline the importance of research into the genetic diversity of both wild and farmed stocks. We aimed to decipher the genetic structure of
S. dumerili
in the Eastern Mediterranean and the Northeast Atlantic using three types of genetic markers (mitochondrial control region sequences, microsatellites, and single nucleotide polymorphisms, or SNPs) to evaluate the population genetic structure of the species. The results were compared to those of previous studies of the species focusing on the Central Mediterranean and confirmed the presence of two divergent mtDNA haplogroups distributed without any detectable geographical structuring within the Mediterranean and the Atlantic. However, population analyses of the genetic structure of the species using either 10 microsatellites or a set of 1,051 SNP markers suggest that the species forms three genetically distinct groups, one in the Mediterranean and two in the Atlantic. The latter has so far not been reported in the Northeast Atlantic, and that this differentiation refers to samples from the Canary Islands is surprising. Fifteen candidate outlier SNP loci were identified in the data set, one located within the
tead1a
/TEF-1A gene, which has been associated with temperature acclimation of the medaka. This work enriches our knowledge of the genetic diversity of wild populations of the greater amberjack in the Mediterranean and the Northeast Atlantic and attempts to investigate signs of local adaptation toward a better understanding of the species distribution patterns. Future studies should be conducted to investigate the genomic regions associated with temperature acclimation in marine organisms that have to adapt to the ongoing climate change pressures, such as sea temperature rise, in order to survive.

Fakriadis, Ioannis; Meiri-Ashkenazi, Iris; Bracha, Chen; Rosenfeld, Hanna; Corriero, Aldo; Zupa, Rosa; Pousis, Chrysovalentinos; Papadaki, Maria; Mylonas, Constantinos C

Gonadotropin expression, pituitary and plasma levels in the reproductive cycle of wild and captive-reared greater amberjack (Seriola dumerili) Journal Article

General and Comparative Endocrinology, 350 , pp. 114465, 2024, ISSN: 00166480.

Links | BibTeX

Ventriglia, G; Fakriadis, I; Papadaki, M; Zupa, R; Pousis, C; Mandalakis, M; Corriero, A; Mylonas, C C

Effects of different hormonal treatments on spermatogenesis advancement in hatchery-produced greater amberjack Seriola dumerili (Risso 1810) Journal Article

348 , pp. 114447, 2024, ISSN: 00166480.

Links | BibTeX

Lancerotto, Stefano; Fakriadis, Ioannis; Papadaki, Maria; Mandalakis, Manolis; Sigelaki, Irini; Mylonas, Constantinos C

Timing of puberty in F1-generation hatchery-produced greater amberjack (Seriola dumerili) Journal Article

General and Comparative Endocrinology, 347 , pp. 114414, 2024, ISSN: 00166480.

Links | BibTeX

2021

Fakriadis, Ioannis; Mylonas, Constantinos C

Sperm quality of greater amberjack Seriola dumerili throughout the reproductive season and in response to GnRHa treatment with controlled release implants Journal Article

Fish Physiology and Biochemistry, 47 (2), pp. 281–292, 2021, ISSN: 1573-5168.

Abstract | Links | BibTeX

@article{fakriadis_sperm_2021,
title = {Sperm quality of greater amberjack Seriola dumerili throughout the reproductive season and in response to GnRHa treatment with controlled release implants},
author = {Ioannis Fakriadis and Constantinos C Mylonas},
url = {https://doi.org/10.1007/s10695-020-00910-9},
doi = {10.1007/s10695-020-00910-9},
issn = {1573-5168},
year = {2021},
date = {2021-01-01},
urldate = {2021-04-27},
journal = {Fish Physiology and Biochemistry},
volume = {47},
number = {2},
pages = {281--292},
abstract = {A wild-caught stock of greater amberjack Seriola dumerili reared in sea cages in two commercial aquaculture facilities in Greece was administered gonadotropin-releasing hormone agonist (GnRHa), loaded in controlled release implants, and was used in spawning induction experiments throughout the reproductive season. Sperm quality was evaluated using computer-assisted sperm analysis (CASA) before and after GnRHa administration and the extent of the spermiation period was determined. Males were in spermiation throughout the monitoring period from May 30 until July 18, at temperatures between 19 and 24 °C. However, lower sperm motility duration, density, and survival under cold storage were observed from early July onwards. Sperm quality did not recover after the end of spawning induction experiments in tanks and the return of the fish for 14–28 days to the sea cage in mid-July, which could be related to the high temperatures of this period. An improvement trend was observed in the quality of the milt collected on day 7 after a single GnRHa administration, but a significant decrease was observed on day 21 in sperm density, survival under cold storage, and straight line velocity (VSL). On the contrary, a double GnRHa administration spaced 14 days apart maintained the same sperm quality for a longer period of 29 days. Further spermiation enhancement experiments should be conducted in the future to describe in more detail the kinetics of sperm production after GnRHa therapy and its effects on sperm quality.},
keywords = {},
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tppubtype = {article}
}

Mello, Paulo De H; Lancerotto, Stefano; Fakriadis, Ioannis; Tsoukali, Panagiota; Papadaki, Maria; Mylonas, Constantinos Chrysanthos

The importance of thermoperiod for proper gametogenesis and successful egg and sperm production in meagre (Argyrosomus regius) breeders in aquaculture Journal Article

Mediterranean Marine Science, 22 (2), pp. 218–231, 2021, ISSN: 1791-6763.

Abstract | Links | BibTeX

@article{mello_importance_2021,
title = {The importance of thermoperiod for proper gametogenesis and successful egg and sperm production in meagre (Argyrosomus regius) breeders in aquaculture},
author = {Paulo De H Mello and Stefano Lancerotto and Ioannis Fakriadis and Panagiota Tsoukali and Maria Papadaki and Constantinos Chrysanthos Mylonas},
url = {https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/25806
https://imbbc.hcmr.gr/wp-content/uploads/2021/05/2021-De-Mello-MMS-27-2.pdf},
doi = {10.12681/mms.25806},
issn = {1791-6763},
year = {2021},
date = {2021-01-01},
urldate = {2021-04-27},
journal = {Mediterranean Marine Science},
volume = {22},
number = {2},
pages = {218--231},
abstract = {We examined the effect of constant water temperature throughout the year on gametogenesis, spawning success and egg/sperm/embryo quality in meagre (Argyrosomus regius). Two broodstocks were exposed to simulated natural photoperiod, andeither attenuated seasonal water temperature (SeasT, 16.4 to 19.6ºC) or relatively constant water temperature (CoT, 19.4 ± 0.6ºC).In the spawning period (May), 4 couples per group were induced to spawn with gonadotropin releasing hormone agonist (GnRHa). Gonadal stage of development, sperm quality parameters and plasma levels of sex steroids were evaluated prior to the GnRHa treatment. Spawning success and egg/sperm quality were examined over the following 4 weeks. Constant temperature did not prevent gametogenesis, but exposure to attenuated seasonal water temperature with the inclusion of winter low temperature was beneficial to both sexes. The mean (±SD) diameter of the largest vitellogenic oocytes prior to GnRHa administration was significantly higher in the SeasT compared to the CoT group (598 ± 27 vs 520 ± 17 μm). Testosterone plasma levels in the females were significantly higher in the SeasT group, but all other hormones were similar in both sexes. SeasT females spawned more consistently with higher relative fecundity, and 24-h embryo survival of the produced eggs. A more pronounced negative effect of constant water temperature was observed in males, since CoT males exhibited a spermiation index of 0 prior to GnRHa treatment, the latter clearly having a beneficial effect over the following 4 weeks. The study demonstrated that meagre do undergo gametogenesis to a significant extent even under constant water temperatures during the year. However, a seasonal thermal regime -even an attenuated one- was necessary for the proper development of the gametes, allowing for the successful spawning induction using the established GnRHa induction protocol.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Superio, Joshua; Fakriadis, Ioannis; Tsigenopoulos, Costas S; Lancerotto, Stefano Adam; Rodriguez, Andrea Villena; Vervelakis, Emanuele; Mylonas, Constantinos C

Spawning kinetics and parentage contribution of European sea bass (Dicentrarchus labrax) broodstocks, and influence of GnRHa-induced spawning Journal Article

Aquaculture Reports, 21 , pp. 100766, 2021, ISSN: 23525134.

Links | BibTeX

2020

Fakriadis, Ioannis; Zanatta, Eugenio Maria; Fleck, Renata Pontes Dos Santos; Mateo, Daybet Lorena Sena; Papadaki, Maria; Mylonas, Constantinos C

Endocrine regulation of long-term enhancement of spermiation in meagre (Argyrosomus regius) with GnRHa controlled-delivery systems Journal Article

General and Comparative Endocrinology, 297 , pp. 113549, 2020, ISSN: 00166480.

Links | BibTeX

Fakriadis, Ioannis; Miccoli, Andrea; Karapanagiotis, Stelios; Tsele, Nikoleta; Mylonas, Constantinos C

Optimization of a GnRHa treatment for spawning commercially reared greater amberjack Seriola dumerili: Dose response and extent of the reproductive season Journal Article

Aquaculture, 521 , 2020, ISSN: 00448486, (Type: Journal Article).

Links | BibTeX

Fakriadis, Ioannis; Sigelaki, Irini; Papadaki, Maria; Papandroulakis, Nikos; Raftopoulos, Anastasios; Tsakoniti, Kalliopi; Mylonas, Constantinos C

Control of reproduction of greater amberjack Seriola dumerili reared in aquaculture facilities Journal Article

Aquaculture, 519 , 2020, ISSN: 00448486, (Type: Journal Article).

Links | BibTeX

@article{fakriadis_control_2020,
title = {Control of reproduction of greater amberjack Seriola dumerili reared in aquaculture facilities},
author = {Ioannis Fakriadis and Irini Sigelaki and Maria Papadaki and Nikos Papandroulakis and Anastasios Raftopoulos and Kalliopi Tsakoniti and Constantinos C Mylonas},
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doi = {10.1016/j.aquaculture.2019.734880},
issn = {00448486},
year = {2020},
date = {2020-01-01},
journal = {Aquaculture},
volume = {519},
note = {Type: Journal Article},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

2019

Pérez, Evaristo; Linares, Fátima; Villanueva, José Luis Rodríguez; Vilar, Antonio; Mylonas, Constantinos C; Fakriadis, Ioannis; Papadaki, Maria; Papandroulakis, Nikos; Papadakis, Ioannis; Robles, Rocío; Fauvel, Christian; Roo, Javier; Peleteiro, José Benito; Lluch, Nuria; Pazos, Gema; Méndez, Belén; Sigelaki, Irini; Gómez, Castora; Pérez, Montse; Álvarez-Blázquez, Blanca

Wreckfish (Polyprion americanus). New Knowledge About Reproduction, Larval Husbandry, and Nutrition. Promise as a New Species for Aquaculture Journal Article

Fishes, 4 (1), pp. 14, 2019, (Type: Journal Article).

BibTeX

Forner-Piquer, Isabel; Fakriadis, Ioannis; Mylonas, Constantinos C; Piscitelli, Fabiana; Marzo, Vincenzo Di; Maradonna, Francesca; Calduch-Giner, Josep; Pérez-Sánchez, Jaume; Carnevali, Oliana

Effects of Dietary Bisphenol A on the Reproductive Function of Gilthead Sea Bream (Sparus aurata) Testes Journal Article

International Journal of Molecular Sciences, 20 (20), pp. 5003, 2019, (Type: Journal Article).

Links | BibTeX

Forner-Piquer, Isabel; Mylonas, Constantinos C; Fakriadis, Ioannis; Papadaki, Maria; Piscitelli, Fabiana; Marzo, Vincenzo Di; Calduch-Giner, Josep; Pérez-Sánchez, Jaume; Carnevali, Oliana

Effects of diisononyl phthalate (DiNP) on the endocannabinoid and reproductive systems of male gilthead sea bream (Sparus aurata) during the spawning season Journal Article

Archives of Toxicology, 2019, ISSN: 1432-0738, (Type: Journal Article).

Links | BibTeX

Fakriadis, I; Lisi, F; Sigelaki, I; Papadaki, M; Mylonas, C C

Spawning kinetics and egg/larval quality of greater amberjack (Seriola dumerili) in response to multiple GnRHa injections or implants Journal Article

General and Comparative Endocrinology, 279 , pp. 78–87, 2019, ISSN: 1095-6840 (Electronic) 0016-6480 (Linking), (Type: Journal Article).

Links | BibTeX

2018

Pousis, C; Mylonas, C C; Virgilio, De C; Gadaleta, G; Santamaria, N; Passantino, L; Zupa, R; Papadaki, M; Fakriadis, I; Ferreri, R; Corriero, A

The observed oogenesis impairment in greater amberjack Seriola dumerili (Risso, 1810) reared in captivity is not related to an insufficient liver transcription or oocyte uptake of vitellogenin Journal Article

Aquaculture Research, 49 (1), pp. 243–252, 2018, ISSN: 1355557X, (Publisher: Blackwell Publishing Ltd).

Abstract | Links | BibTeX

@article{pousis_observed_2018,
title = {The observed oogenesis impairment in greater amberjack Seriola dumerili (Risso, 1810) reared in captivity is not related to an insufficient liver transcription or oocyte uptake of vitellogenin},
author = {C Pousis and C C Mylonas and C De Virgilio and G Gadaleta and N Santamaria and L Passantino and R Zupa and M Papadaki and I Fakriadis and R Ferreri and A Corriero},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85026499054&doi=10.1111%2fare.13453&partnerID=40&md5=ecb8f6c6b9bbbf8b803322299447cfd4},
doi = {10.1111/are.13453},
issn = {1355557X},
year = {2018},
date = {2018-01-01},
journal = {Aquaculture Research},
volume = {49},
number = {1},
pages = {243--252},
abstract = {The greater amberjack Seriola dumerili is an excellent candidate for the Mediterranean aquaculture, due to its large body size and high growth rate, as well as its high flesh quality and commercial value worldwide. For its successful incorporation in the aquaculture industry, an in-depth understanding of the reproductive function of the species under rearing conditions is necessary, since completion of oogenesis in captivity is currently a bottleneck for the commercial production of the species. Liver and ovary samples from wild and captive-reared greater amberjack females were collected at three different phases of the reproductive cycle: early gametogenesis (EARLY, late April-early May), advanced gametogenesis (ADVANCED, late May-early June) and spawning (SPAWNING, late June-July). The cDNAs of three vitellogenins (VtgA, VtgB and VtgC) were partially sequenced and a qRT-PCR for their expression was used to compare ovarian maturity stage and liver vitellogenin transcript levels between wild and captive-reared individuals. An extensive atresia of late vitellogenic follicles, which prevented any further oocyte development and spawning was observed in captive-reared individuals during the ADVANCED phase. The expression levels of the three vitellogenins, as well as the amount of yolk globules in vitellogenic oocytes, did not differ significantly between captive-reared and wild females, indicating that the observed oogenesis impairment in greater amberjack reared in captivity was not related to an insufficient liver synthesis or a reduced oocyte uptake of vitellogenin. © 2017 John Wiley & Sons Ltd},
note = {Publisher: Blackwell Publishing Ltd},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Papadaki, M; Mazzella, D; Santinelli, V; Fakriadis, I; Sigelaki, I; Mylonas, C C

Hermaphroditism and reproductive function of hatchery-produced sharpsnout seabream (Diplodus puntazzo) under attenuated annual thermal cycles Journal Article

Aquaculture, 482 , pp. 231–240, 2018, ISSN: 00448486, (Publisher: Elsevier B.V.).

Abstract | Links | BibTeX

@article{papadaki_hermaphroditism_2018,
title = {Hermaphroditism and reproductive function of hatchery-produced sharpsnout seabream (Diplodus puntazzo) under attenuated annual thermal cycles},
author = {M Papadaki and D Mazzella and V Santinelli and I Fakriadis and I Sigelaki and C C Mylonas},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85030865182&doi=10.1016%2fj.aquaculture.2017.09.028&partnerID=40&md5=61ad82357d509cb8f19491299ca570e0},
doi = {10.1016/j.aquaculture.2017.09.028},
issn = {00448486},
year = {2018},
date = {2018-01-01},
journal = {Aquaculture},
volume = {482},
pages = {231--240},
abstract = {Acquiring more knowledge on the reproductive biology of the sharpsnout seabream (Diplodus puntazzo) can improve our ability to control its reproduction and enable hatcheries to reconsider this fish as a potential species for the Mediterranean aquaculture production. We investigated hermaphroditism and reproductive function in a hatchery-produced broodstock over a long period of 6 years, which includes sex differentiation, puberty and four consecutive annual reproductive seasons, at relatively constant water temperatures of 18–20 °C, which are typical of borehole seawater supplies used frequently in marine hatcheries for biosecurity reasons. At the end of the first year of life, the gonads were bisexual and were classified into three different categories: mainly testicular tissue with rudimentary ovarian tissue (Mf), substantial amounts of both tissues (MF) and mainly ovarian tissue with rudimentary testicular tissue (mF). Fully female (F) gonads appeared already in 1 + years old fish, whereas fully male (M) gonads appeared for the first time in 2 + years old fish. The sex ratio (F:M) of the studied populations during the first 6 years of life in all age classes was not different from 1:1. Therefore, no annual restructuring of the broodstock is necessary in order to avoid the development of skewed sex ratios, since once functional sex is established it does not seem to change in the following years. Furthermore, the use of attenuated annual thermal cycling regimes did not seem to affect the annual gametogenic cycle and full maturation of this species, though spontaneous spawning was still missing and the fish exhibited the typical problems reported for this species in captivity. © 2017 Elsevier B.V.},
note = {Publisher: Elsevier B.V.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Jerez, Salvador; Fakriadis, Ioannis; Papadaki, Maria; Martín, M; Cejas, Juana; Mylonas, Constantinos C

Spawning induction of first-generation (F1) greater amberjack Seriola dumerili in the Canary Islands, Spain using GnRHa delivery systems Journal Article

Fishes, 3 (3), pp. 1–22, 2018, ISSN: 2410-3888, (Type: Journal Article).

Links | BibTeX

Papadaki, Maria; Peleteiro, José Benito; Alvarez-Blázquez, Blanca; Villanueva, José Luis Rodríguez; Linares, Fatima; Vilar, Antonio; Rial, Evaristo Pérez; Lluch, Nuria; Fakriadis, Ioannis; Sigelaki, Irini; Mylonas, Constantinos C

Description of the Annual Reproductive Cycle of Wreckfish Polyprion americanus in Captivity Journal Article

Fishes, 3 (4), pp. 1–20, 2018, ISSN: 2410-3888, (Type: Journal Article).

Links | BibTeX

2017

Zupa, R; Rodrõâguez, C; Mylonas, C C; Rosenfeld, H; Fakriadis, I; Papadaki, M; Peârez, J A; Pousis, C; Basilone, G; Corriero, A

Comparative study of reproductive development in wild and captive-reared greater amberjack seriola dumerili (Risso, 1810) Journal Article

PLoS ONE, 12 (1), 2017, ISSN: 19326203, (Publisher: Public Library of Science).

Abstract | Links | BibTeX

@article{zupa_comparative_2017,
title = {Comparative study of reproductive development in wild and captive-reared greater amberjack seriola dumerili (Risso, 1810)},
author = {R Zupa and C Rodrõâguez and C C Mylonas and H Rosenfeld and I Fakriadis and M Papadaki and J A Peârez and C Pousis and G Basilone and A Corriero},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85008686824&doi=10.1371%2fjournal.pone.0169645&partnerID=40&md5=09301e14b368a835887bf0e7176db149},
doi = {10.1371/journal.pone.0169645},
issn = {19326203},
year = {2017},
date = {2017-01-01},
journal = {PLoS ONE},
volume = {12},
number = {1},
abstract = {The greater amberjack Seriola dumerili is a large teleost fish with rapid growth and excellent flesh quality, whose domestication represents an ambitious challenge for aquaculture. The occurrence of reproductive dysfunctions in greater amberjack reared in captivity was investigated by comparing reproductive development of wild and captive-reared individuals. Wild and captive-reared breeders were sampled in the Mediterranean Sea during three different phases of the reproductive cycle: Early gametogenesis (EARLY, late April-early May), advanced gametogenesis (ADVANCED, late May-early June) and spawning (SPAWNING, late June-July). Fish reproductive state was evaluated using the gonado-somatic index (GSI), histological analysis of the gonads and determination of sex steroid levels in the plasma, and correlated with leptin expression in the liver and gonad biochemical composition. The GSI and sex steroid levels were lower in captive-reared than in wild fish. During the ADVANCED period, when the wild greater amberjack breeders were already in spawning condition, ovaries of captive-reared breeders showed extensive atresia of late vitellogenic oocytes and spermatogenic activity ceased in the testes of half of the examined males. During the SPAWNING period, all captive-reared fish had regressed gonads, while wild breeders still displayed reproductive activity. Liver leptin expression and gonad proximate composition of wild and captive greater amberjack were similar. However, the gonads of captive-reared fish showed different total polar lipid contents, as well as specific lipid classes and fatty acid profiles with respect to wild individuals. This study underlines the need for an improvement in rearing technology for this species, which should include minimum handling during the reproductive season and the formulation of a specific diet to overcome the observed gonadal decrements of phospholipids, DHA (22:6n-3) and ARA (20:4n-6), compared to wild breeders. © 2017 Zupa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},
note = {Publisher: Public Library of Science},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

The greater amberjack Seriola dumerili is a large teleost fish with rapid growth and excellent flesh quality, whose domestication represents an ambitious challenge for aquaculture. The occurrence of reproductive dysfunctions in greater amberjack reared in captivity was investigated by comparing reproductive development of wild and captive-reared individuals. Wild and captive-reared breeders were sampled in the Mediterranean Sea during three different phases of the reproductive cycle: Early gametogenesis (EARLY, late April-early May), advanced gametogenesis (ADVANCED, late May-early June) and spawning (SPAWNING, late June-July). Fish reproductive state was evaluated using the gonado-somatic index (GSI), histological analysis of the gonads and determination of sex steroid levels in the plasma, and correlated with leptin expression in the liver and gonad biochemical composition. The GSI and sex steroid levels were lower in captive-reared than in wild fish. During the ADVANCED period, when the wild greater amberjack breeders were already in spawning condition, ovaries of captive-reared breeders showed extensive atresia of late vitellogenic oocytes and spermatogenic activity ceased in the testes of half of the examined males. During the SPAWNING period, all captive-reared fish had regressed gonads, while wild breeders still displayed reproductive activity. Liver leptin expression and gonad proximate composition of wild and captive greater amberjack were similar. However, the gonads of captive-reared fish showed different total polar lipid contents, as well as specific lipid classes and fatty acid profiles with respect to wild individuals. This study underlines the need for an improvement in rearing technology for this species, which should include minimum handling during the reproductive season and the formulation of a specific diet to overcome the observed gonadal decrements of phospholipids, DHA (22:6n-3) and ARA (20:4n-6), compared to wild breeders. © 2017 Zupa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Zupa, R; Fauvel, C; Mylonas, C C; Pousis, C; Santamaria, N; Papadaki, Μ; Fakriadis, I; Cicirelli, V; Mangano, S; Passantino, L; Lacalandra, G M; Corriero, A

Rearing in captivity affects spermatogenesis and sperm quality in greater amberjack, Seriola dumerili (Risso, 1810) Journal Article

Journal of Animal Science, 95 (9), pp. 4085–4100, 2017, ISSN: 00218812, (Publisher: American Society of Animal Science).

Abstract | Links | BibTeX

@article{zupa_rearing_2017,
title = {Rearing in captivity affects spermatogenesis and sperm quality in greater amberjack, Seriola dumerili (Risso, 1810)},
author = {R Zupa and C Fauvel and C C Mylonas and C Pousis and N Santamaria and Μ Papadaki and I Fakriadis and V Cicirelli and S Mangano and L Passantino and G M Lacalandra and A Corriero},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85029717001&doi=10.2527%2fjas.2017.1708&partnerID=40&md5=93369a73080df336a351b6a9a006cab1},
doi = {10.2527/jas.2017.1708},
issn = {00218812},
year = {2017},
date = {2017-01-01},
journal = {Journal of Animal Science},
volume = {95},
number = {9},
pages = {4085--4100},
abstract = {The greater amberjack, Seriola dumerili (Risso, 1810), is a promising candidate for the diversification of European aquaculture production, but inconsistent reproduction in captivity prevents commercial production. Recent studies showed that greater amberjack confined in sea cages exhibited scarce gonad development and early interruption of gametogenic activity during the reproductive season. The aim of the present study was to improve our understanding of the observed impairment of spermatogenesis. Adult wild and captive-reared males were sampled during 3 different phases of the reproductive cycle: early gametogenesis (EARLY; late April to early May), advanced gametogenesis (ADVANCED; late May to early June), and spawning (SPAWNING; late June to July). Spermatogonial stem cells and proliferating germ cells were identified through the immunohistochemical localization of Pou5f1 and proliferating cell nuclear antigen, respectively. Apoptotic germ cells were identified throughout the terminal deoxynucleotidyl transferase-mediated 2’-deoxyuridine 5’-triphosphate nick end labeling method. Sperm quality of captive-reared fish was evaluated using computer-assisted sperm analysis. Captive-reared males exhibited seminiferous lobules of a smaller diameter, a precocious and progressive decrease of spermatogonial mitosis, and a high level of apoptosis at the beginning of the reproductive season, concomitant with a many-fold higher 17β-estradiol plasma concentration. The motile spermatozoa percentage of captive greater amberjack was lower than in other teleosts, and a drastic decrease of spermatozoa motility duration, velocity, and ATP content occurred along the reproductive season. An abnormal increase of sperm concentration as well as an increase of dead spermatozoa occurred during the SPAWNING phase, probably because of lack of sperm hydration and ejaculation and consequent sperm ageing. The present study demonstrates the extreme susceptibility of greater amberjack to rearing stress and underscores the need for improvement of the rearing and handling procedures to ameliorate gametogenesis dysfunctions in commercial aquaculture production. © 2017 American Society of Animal Science. All rights reserved.},
note = {Publisher: American Society of Animal Science},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

The greater amberjack, Seriola dumerili (Risso, 1810), is a promising candidate for the diversification of European aquaculture production, but inconsistent reproduction in captivity prevents commercial production. Recent studies showed that greater amberjack confined in sea cages exhibited scarce gonad development and early interruption of gametogenic activity during the reproductive season. The aim of the present study was to improve our understanding of the observed impairment of spermatogenesis. Adult wild and captive-reared males were sampled during 3 different phases of the reproductive cycle: early gametogenesis (EARLY; late April to early May), advanced gametogenesis (ADVANCED; late May to early June), and spawning (SPAWNING; late June to July). Spermatogonial stem cells and proliferating germ cells were identified through the immunohistochemical localization of Pou5f1 and proliferating cell nuclear antigen, respectively. Apoptotic germ cells were identified throughout the terminal deoxynucleotidyl transferase-mediated 2’-deoxyuridine 5’-triphosphate nick end labeling method. Sperm quality of captive-reared fish was evaluated using computer-assisted sperm analysis. Captive-reared males exhibited seminiferous lobules of a smaller diameter, a precocious and progressive decrease of spermatogonial mitosis, and a high level of apoptosis at the beginning of the reproductive season, concomitant with a many-fold higher 17β-estradiol plasma concentration. The motile spermatozoa percentage of captive greater amberjack was lower than in other teleosts, and a drastic decrease of spermatozoa motility duration, velocity, and ATP content occurred along the reproductive season. An abnormal increase of sperm concentration as well as an increase of dead spermatozoa occurred during the SPAWNING phase, probably because of lack of sperm hydration and ejaculation and consequent sperm ageing. The present study demonstrates the extreme susceptibility of greater amberjack to rearing stress and underscores the need for improvement of the rearing and handling procedures to ameliorate gametogenesis dysfunctions in commercial aquaculture production. © 2017 American Society of Animal Science. All rights reserved.

2016

Flack, A; Fiedler, W; Blas, J; Pokrovsky, I; Kaatz, M; Mitropolsky, M; Aghababyan, K; Fakriadis, I; Makrigianni, E; Jerzak, L; Azafzaf, H; Feltrup-Azafzaf, C; Rotics, S; Mokotjomela, T M; Nathan, R; Wikelski, M

Costs of migratory decisions: A comparison across eight white stork populations Journal Article

Science Advances, 2 (1), 2016, ISSN: 23752548, (Publisher: American Association for the Advancement of Science).

Abstract | Links | BibTeX

@article{flack_costs_2016,
title = {Costs of migratory decisions: A comparison across eight white stork populations},
author = {A Flack and W Fiedler and J Blas and I Pokrovsky and M Kaatz and M Mitropolsky and K Aghababyan and I Fakriadis and E Makrigianni and L Jerzak and H Azafzaf and C Feltrup-Azafzaf and S Rotics and T M Mokotjomela and R Nathan and M Wikelski},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84974545850&doi=10.1126%2fsciadv.1500931&partnerID=40&md5=512900f1a33f4132dce13117d925ce50},
doi = {10.1126/sciadv.1500931},
issn = {23752548},
year = {2016},
date = {2016-01-01},
journal = {Science Advances},
volume = {2},
number = {1},
abstract = {Annual migratory movements can range from a few tens to thousands of kilometers, creating unique energetic requirements for each specific species and journey. Even within the same species, migration costs can vary largely because of flexible, opportunistic life history strategies. We uncover the large extent of variation in the lifetime migratory decisions of young white storks originating from eight populations. Not only did juvenile storks differ in their geographically distinct wintering locations, their diverse migration patterns also affected the amount of energy individuals invested for locomotion during the first months of their life. Overwintering in areas with higher human population reduced the stork's overall energy expenditure because of shorter daily foraging trips, closer wintering grounds, or a complete suppression of migration. Because migrants can change ecological processes in several distinct communities simultaneously, understanding their life history decisions helps not only to protect migratory species but also to conserve stable ecosystems. © The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science.},
note = {Publisher: American Association for the Advancement of Science},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Mylonas, C C; Salone, S; Biglino, T; de Mello, P H; Fakriadis, I; Sigelaki, I; Duncan, N

Enhancement of oogenesis/spermatogenesis in meagre Argyrosomus regius using a combination of temperature control and GnRHa treatments Journal Article

Aquaculture, 464 , pp. 323–330, 2016, ISSN: 00448486, (Publisher: Elsevier B.V.).

Abstract | Links | BibTeX

@article{mylonas_enhancement_2016,
title = {Enhancement of oogenesis/spermatogenesis in meagre Argyrosomus regius using a combination of temperature control and GnRHa treatments},
author = {C C Mylonas and S Salone and T Biglino and P H de Mello and I Fakriadis and I Sigelaki and N Duncan},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84978193838&doi=10.1016%2fj.aquaculture.2016.07.006&partnerID=40&md5=33e0a7321455743f1b6c84fcc8aedb38},
doi = {10.1016/j.aquaculture.2016.07.006},
issn = {00448486},
year = {2016},
date = {2016-01-01},
journal = {Aquaculture},
volume = {464},
pages = {323--330},
abstract = {A multiple GnRHa injection method for inducing spawning of high fecundity and quality in meagre was optimized. Specifically, the study (a) examined how long females would continue spawning in response to consecutive, weekly GnRHa injections, if maintained at the natural spawning temperature, and (b) evaluated whether males can produce adequate amounts of good quality sperm for the same period, with the assistance of a GnRHa therapy. Combined with stable temperatures (19–20 °C) that are prevalent during the early spawning season (April–May) in the Mediterranean Sea, each weekly GnRHa injection induced 2 consecutive spawns per week for a period of 17 weeks. Fish spawned consistently 2 days after treatment and produced high fecundity and egg quality (i.e. fertilization, hatching and early larval development). Maximum mean total fecundity (± S.D.) obtained was 1,415,000 ± 149,000 eggs kg− 1 in 32 spawns, being the highest total season fecundity reported for meagre, and significantly higher than the estimated maximum potential fecundity for the species. At the same time, sperm of consistently high quality (i.e. initial motility, duration of motility and storage survival) was produced in response to treatment with multiple GnRHa implants, though towards the end of the experiment a significant reduction (ANOVA, P ≤ 0.05) in spermatozoa density was observed. This protocol is expected to enhance greatly commercial production of meagre. Statement of relevance The present study reports on an optimized protocol for the induction of consistent and repeated spawning in meagre, based on the use of weekly GnRHa injections combined with stable temperatures that are prevalent at the early spawning season in the Mediterranean Sea. This protocol produced eggs of high fecundity and quality (i.e. fertilization, hatching and early larval development) for a period of > 4 months, and it is expected to enhance greatly commercial production of meagre. © 2016},
note = {Publisher: Elsevier B.V.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

2008

Peñaranda, D S; Pérez, L; Fakriadis, G; Mylonas, C C; Asturiano, J F

Effects of extenders and cryoprotectant combinations on motility and morphometry of sea bass (Dicentrarchus labrax) spermatozoa Journal Article

Journal of Applied Ichthyology, 24 (4), pp. 450–455, 2008, ISSN: 01758659.

Abstract | Links | BibTeX