2025
Kotsiri, Mado; Kogiannou, Dimitra; Nikoloudaki, Chrisanthi; Kleidas, Ioannis; Dogrammatzi, Aikaterini; Karachle, Paraskevi K; Grigorakis, Kriton
Seasonal Variation in Nutritional Value and Technical Quality of Lionfish (Pterois miles) from the Ionian and Aegean Seas Journal Article
In: Foods, vol. 14, no. 13, pp. 2353, 2025, ISSN: 2304-8158.
@article{kotsiri_seasonal_2025,
title = {Seasonal Variation in Nutritional Value and Technical Quality of Lionfish (Pterois miles) from the Ionian and Aegean Seas},
author = {Mado Kotsiri and Dimitra Kogiannou and Chrisanthi Nikoloudaki and Ioannis Kleidas and Aikaterini Dogrammatzi and Paraskevi K Karachle and Kriton Grigorakis},
url = {https://www.mdpi.com/2304-8158/14/13/2353
/wp-content/uploads/2025/07/2025-Kotsiri-foods-37.pdf
},
doi = {10.3390/foods14132353},
issn = {2304-8158},
year = {2025},
date = {2025-07-18},
urldate = {2025-07-18},
journal = {Foods},
volume = {14},
number = {13},
pages = {2353},
abstract = {Lionfish (Pterois miles), an invasive species in the Mediterranean, pose ecological and socioeconomic challenges. This study examines the seasonal variation in the nutritional composition and technical quality of lionfish from the Ionian and Aegean Seas, evaluating their potential as a food resource. Fillets were high in protein (19.4%) and low in fat (2.0%), with significant seasonal differences in the Ionian Sea, where winter samples had higher lipid content. The fillet yield (28.4%) was satisfactory given the fish’s morphology. Fatty acid analysis confirmed lionfish as a valuable source of polyunsaturated fatty acids (PUFAs), particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), with EPA + DHA levels exceeding the recommended daily intake (119.2%). Seasonal variations in fatty acid composition were observed, including higher EPA in autumn and lower lipid nutritional quality in summer. Arachidonic acid (ARA) was also present at nutritionally significant levels (79.4 mg/100 g). The n3/n6 ratio (2.2) and favourable atherogenic and thrombogenic indices highlight its nutritional benefits. This is the first study to assess seasonal variations in the nutritional value and technical yield of lionfish in the Mediterranean, offering novel insights into its commercial valorisation. These findings support its promotion as a sustainable protein source and as a means of managing its invasive population.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Lionfish (Pterois miles), an invasive species in the Mediterranean, pose ecological and socioeconomic challenges. This study examines the seasonal variation in the nutritional composition and technical quality of lionfish from the Ionian and Aegean Seas, evaluating their potential as a food resource. Fillets were high in protein (19.4%) and low in fat (2.0%), with significant seasonal differences in the Ionian Sea, where winter samples had higher lipid content. The fillet yield (28.4%) was satisfactory given the fish’s morphology. Fatty acid analysis confirmed lionfish as a valuable source of polyunsaturated fatty acids (PUFAs), particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), with EPA + DHA levels exceeding the recommended daily intake (119.2%). Seasonal variations in fatty acid composition were observed, including higher EPA in autumn and lower lipid nutritional quality in summer. Arachidonic acid (ARA) was also present at nutritionally significant levels (79.4 mg/100 g). The n3/n6 ratio (2.2) and favourable atherogenic and thrombogenic indices highlight its nutritional benefits. This is the first study to assess seasonal variations in the nutritional value and technical yield of lionfish in the Mediterranean, offering novel insights into its commercial valorisation. These findings support its promotion as a sustainable protein source and as a means of managing its invasive population.
Grigorakis, Kriton; Kogiannou, Dimitra; Kotsiri, Mado; Kleidas, Ioannis; Mello, Paulo H De; Habiballah, Salaheldeen; Alshaikhi, Ali; Alhafedh, Youssef S; Mohamed, Asaad H W
Freshness and Spoilage Patterns of Wild and Farmed Tropical Fish Species with Major Commercial Importance Originating from Saudi Arabian Waters Journal Article
In: Foods, vol. 14, no. 4, pp. 690, 2025, ISSN: 2304-8158.
@article{grigorakis_freshness_2025,
title = {Freshness and Spoilage Patterns of Wild and Farmed Tropical Fish Species with Major Commercial Importance Originating from Saudi Arabian Waters},
author = {Kriton Grigorakis and Dimitra Kogiannou and Mado Kotsiri and Ioannis Kleidas and Paulo H De Mello and Salaheldeen Habiballah and Ali Alshaikhi and Youssef S Alhafedh and Asaad H W Mohamed},
url = {https://www.mdpi.com/2304-8158/14/4/690
/wp-content/uploads/2025/02/2025-Grigorakis-foods-8.pdf},
doi = {10.3390/foods14040690},
issn = {2304-8158},
year = {2025},
date = {2025-02-24},
urldate = {2025-02-24},
journal = {Foods},
volume = {14},
number = {4},
pages = {690},
abstract = {Ice-stored farmed barramundi (Lates calcarifer), snubnose pompano (Trachinotus blochii) and sobaity bream (Sparidentex hasta), as well as wild-caught cobia (Rachycentron canadum), coral trout (Plectropomus leopardus), giant trevally (Caranx ignobilis), milkfish (Chanos chanos) and mangrove red snapper (Lutjanus argentimaculatus), were compared for their freshness/spoilage using sensory, chemical and microbiological methods. Quality Index Method schemes were developed to determine alterations in the sensory freshness. The shelf lives ranged from 8 (coral trout) to 18 days (sobaity bream). The farmed species always exhibited a significantly longer shelf life than the wild-caught species. The adenosine triphosphate (ATP) breakdown followed different patterns in the studied species. The K-values at the time of sensory rejection ranged from 30 to 80% depending on the species, while the microbial load reached or exceeded a level of 6 log cfu/g. Although the shelf life duration was dependent on the origin of the fish (wild or farmed), the ATP breakdown scheme, as well as the K-values and microbial loads at the time of rejection, were species-dependent and independent of the origin.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Ice-stored farmed barramundi (Lates calcarifer), snubnose pompano (Trachinotus blochii) and sobaity bream (Sparidentex hasta), as well as wild-caught cobia (Rachycentron canadum), coral trout (Plectropomus leopardus), giant trevally (Caranx ignobilis), milkfish (Chanos chanos) and mangrove red snapper (Lutjanus argentimaculatus), were compared for their freshness/spoilage using sensory, chemical and microbiological methods. Quality Index Method schemes were developed to determine alterations in the sensory freshness. The shelf lives ranged from 8 (coral trout) to 18 days (sobaity bream). The farmed species always exhibited a significantly longer shelf life than the wild-caught species. The adenosine triphosphate (ATP) breakdown followed different patterns in the studied species. The K-values at the time of sensory rejection ranged from 30 to 80% depending on the species, while the microbial load reached or exceeded a level of 6 log cfu/g. Although the shelf life duration was dependent on the origin of the fish (wild or farmed), the ATP breakdown scheme, as well as the K-values and microbial loads at the time of rejection, were species-dependent and independent of the origin.
2024
Kotsiri, Mado; Kleidas, Ioannis; Kogiannou, Dimitra; Vryonides, Katerina; Papadouli, Christina; Golomazou, Eleni; Rigos, George
Evaluation of the long-term effects of formaldehyde on the physiology of the Mediterranean mussel, Mytilus galloprovincialis Journal Article
In: Chemosphere, vol. 364, pp. 143190, 2024, ISSN: 00456535.
@article{kotsiri_evaluation_2024,
title = {Evaluation of the long-term effects of formaldehyde on the physiology of the Mediterranean mussel, Mytilus galloprovincialis},
author = {Mado Kotsiri and Ioannis Kleidas and Dimitra Kogiannou and Katerina Vryonides and Christina Papadouli and Eleni Golomazou and George Rigos},
url = {https://linkinghub.elsevier.com/retrieve/pii/S0045653524020873
https://imbbc.hcmr.gr/wp-content/uploads/2024/10/2024-Kotsiri-43-pre-print.pdf},
doi = {10.1016/j.chemosphere.2024.143190},
issn = {00456535},
year = {2024},
date = {2024-09-30},
urldate = {2024-10-01},
journal = {Chemosphere},
volume = {364},
pages = {143190},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
2023
Kogiannou, Dimitra; Nikoloudaki, Chrysanthi; Valsamidi, Vasilia; Kotsiri, Mado; Golomazou, Eleni; Rigos, George
Pharmacokinetics of praziquantel in gilthead seabream (Sparus aurata) plasma and gills Journal Article
In: Aquaculture, vol. 577, pp. 739975, 2023, ISSN: 00448486.
@article{kogiannou_pharmacokinetics_2023,
title = {Pharmacokinetics of praziquantel in gilthead seabream (Sparus aurata) plasma and gills},
author = {Dimitra Kogiannou and Chrysanthi Nikoloudaki and Vasilia Valsamidi and Mado Kotsiri and Eleni Golomazou and George Rigos},
url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/09/2023-Kogiannou-AQUA-pre-print45.pdf
https://linkinghub.elsevier.com/retrieve/pii/S0044848623007494},
doi = {10.1016/j.aquaculture.2023.739975},
issn = {00448486},
year = {2023},
date = {2023-09-06},
urldate = {2023-09-07},
journal = {Aquaculture},
volume = {577},
pages = {739975},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Kotsiri, Mado; Panara, Anthi; Koupa, Anastasia; Gourzioti, Evgenia; Thomaidis, Nikolaos S; Rigos, George
Preliminary measurements of formaldehyde in seawater and edible tissues of farmed gilthead seabream after formalin immersion Journal Article
In: Mediterranean Marine Science, vol. 24, no. 2, pp. 384–391, 2023, ISSN: 1791-6763, 1108-393X.
@article{kotsiri_preliminary_2023,
title = {Preliminary measurements of formaldehyde in seawater and edible tissues of farmed gilthead seabream after formalin immersion},
author = {Mado Kotsiri and Anthi Panara and Anastasia Koupa and Evgenia Gourzioti and Nikolaos S Thomaidis and George Rigos},
url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/07/2023-Kotsiri-MMS-39.pdf
https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/33606},
doi = {10.12681/mms.33606},
issn = {1791-6763, 1108-393X},
year = {2023},
date = {2023-07-21},
urldate = {2023-07-21},
journal = {Mediterranean Marine Science},
volume = {24},
number = {2},
pages = {384--391},
abstract = {This study was performed to measure the possible persistence of formaldehyde, the active substance of formalin solution, in seawater and edible gilthead seabream (Sparus aurata) tissues after a formalin bath. The trial was carried out during the summer period as parasitic infestations are of a high frequency. Water samples were taken within or at a short distance from the treatment cages, during and following formalin immersion. Fish fillets were also sampled at the same sampling points. Chromatographic analysis of water and tissue samples was performed with a photodiode array detector (HPLC-PDA). Measured formaldehyde concentrations in gilthead seabream fillets clearly showed no increase due to the immersion or advent of time. Formaldehyde measurements in seawater revealed that after formalin treatments negligible amounts of the substance remain in the aquatic environment and these values seem to be relatively unaffected by depth in the vicinity of the fish cages. Further research is required to investigate the formalin degradation cycle under Mediterranean seawater conditions, including more farm sites and temperature ranges.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
This study was performed to measure the possible persistence of formaldehyde, the active substance of formalin solution, in seawater and edible gilthead seabream (Sparus aurata) tissues after a formalin bath. The trial was carried out during the summer period as parasitic infestations are of a high frequency. Water samples were taken within or at a short distance from the treatment cages, during and following formalin immersion. Fish fillets were also sampled at the same sampling points. Chromatographic analysis of water and tissue samples was performed with a photodiode array detector (HPLC-PDA). Measured formaldehyde concentrations in gilthead seabream fillets clearly showed no increase due to the immersion or advent of time. Formaldehyde measurements in seawater revealed that after formalin treatments negligible amounts of the substance remain in the aquatic environment and these values seem to be relatively unaffected by depth in the vicinity of the fish cages. Further research is required to investigate the formalin degradation cycle under Mediterranean seawater conditions, including more farm sites and temperature ranges.
Nanou, Evangelia; Kotsiri, Mado; Kogiannou, Dimitra; Katsouli, Maria; Grigorakis, Kriton
In: Foods, vol. 12, no. 3, pp. 505, 2023, ISSN: 2304-8158.
@article{nanou_consumer_2023,
title = {Consumer Perception of Freshness and Volatile Composition of Fresh Gilthead Seabream and Seabass in Active Packaging with and without CO2-Emitting Pads},
author = {Evangelia Nanou and Mado Kotsiri and Dimitra Kogiannou and Maria Katsouli and Kriton Grigorakis},
url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/02/2023-Nanou-foods-10.pdf
https://www.mdpi.com/2304-8158/12/3/505},
doi = {10.3390/foods12030505},
issn = {2304-8158},
year = {2023},
date = {2023-02-14},
urldate = {2023-02-15},
journal = {Foods},
volume = {12},
number = {3},
pages = {505},
abstract = {Active packaging with CO2-emitters (pads) has recently been used for shelf-life extension of fresh fish. The aim of this study was to identify consumer attitudes towards fresh fish packaging, to examine whether Greek consumers prefer active packaging with pad over active packaging without pad, to investigate any perceived differences in the sensory freshness of the fish, and to relate consumer perception to volatile composition of fish fillets. In total, 274 consumers participated in the study which included freshness sensory evaluation of gilthead seabream (Sparus aurata) and seabass (Dicentrarchuslabrax), whole-gutted and filleted, raw and cooked, at high quality and at the end of high-quality shelf-life. Samples were packed under modified atmosphere either with a pad or without. Results showed that consumers preferred packages with pads, especially at the end of high quality shelf-life. They perceived raw samples packed with a pad to be fresher and closer to the ideal product, and also had a higher purchase intention towards them. Cooked samples were not perceived differently. Consumers’ perception was in accordance with the GC-MS findings in the volatile compounds that function as freshness or spoilage indicators. Most participants were positive towards fresh fish packaging although they usually buy unpacked fresh fish. Our results suggest that active packaging with CO2 emitters contribute to freshness preservation and that it has a positive potential in the Greek market.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Active packaging with CO2-emitters (pads) has recently been used for shelf-life extension of fresh fish. The aim of this study was to identify consumer attitudes towards fresh fish packaging, to examine whether Greek consumers prefer active packaging with pad over active packaging without pad, to investigate any perceived differences in the sensory freshness of the fish, and to relate consumer perception to volatile composition of fish fillets. In total, 274 consumers participated in the study which included freshness sensory evaluation of gilthead seabream (Sparus aurata) and seabass (Dicentrarchuslabrax), whole-gutted and filleted, raw and cooked, at high quality and at the end of high-quality shelf-life. Samples were packed under modified atmosphere either with a pad or without. Results showed that consumers preferred packages with pads, especially at the end of high quality shelf-life. They perceived raw samples packed with a pad to be fresher and closer to the ideal product, and also had a higher purchase intention towards them. Cooked samples were not perceived differently. Consumers’ perception was in accordance with the GC-MS findings in the volatile compounds that function as freshness or spoilage indicators. Most participants were positive towards fresh fish packaging although they usually buy unpacked fresh fish. Our results suggest that active packaging with CO2 emitters contribute to freshness preservation and that it has a positive potential in the Greek market.
2022
Kogiannou, Dimitra; Kotsiri, Mado; Grigorakis, Kriton
A method to assess gaping in Sparidae species fillets Journal Article
In: Aquaculture Research, vol. 53, no. 2, pp. 689–693, 2022, ISSN: 1355-557X, 1365-2109.
@article{kogiannou_method_2022,
title = {A method to assess gaping in Sparidae species fillets},
author = {Dimitra Kogiannou and Mado Kotsiri and Kriton Grigorakis},
url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/01/2021-Kogianou-ARE-preprint-88.pdf
https://onlinelibrary.wiley.com/doi/10.1111/are.15590},
doi = {10.1111/are.15590},
issn = {1355-557X, 1365-2109},
year = {2022},
date = {2022-01-01},
urldate = {2022-01-12},
journal = {Aquaculture Research},
volume = {53},
number = {2},
pages = {689--693},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Katsouli, Maria; Semenoglou, Ioanna; Kotsiri, Mado; Gogou, Eleni; Tsironi, Theofania; Taoukis, Petros
In: Foods, vol. 11, no. 15, pp. 2245, 2022, ISSN: 2304-8158.
@article{katsouli_active_2022,
title = {Active and Intelligent Packaging for Enhancing Modified Atmospheres and Monitoring Quality and Shelf Life of Packed Gilthead Seabream Fillets at Isothermal and Variable Temperature Conditions},
author = {Maria Katsouli and Ioanna Semenoglou and Mado Kotsiri and Eleni Gogou and Theofania Tsironi and Petros Taoukis},
url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/08/2022-Kotsiri-Foods-55.pdf
https://www.mdpi.com/2304-8158/11/15/2245},
doi = {10.3390/foods11152245},
issn = {2304-8158},
year = {2022},
date = {2022-01-01},
urldate = {2022-08-26},
journal = {Foods},
volume = {11},
number = {15},
pages = {2245},
abstract = {The study investigated the effect of active modified atmosphere packaging (20% CO2–60% N2–20% O2) with CO2 emitters (MAP-PAD) and conventional MAP (MAP) on the quality and shelf-life of gilthead seabream fillets during chill storage, while the most appropriate enzymatic Time Temperature Integrators (TTI) were selected for monitoring their shelf-life at isothermal and variable temperature storage conditions (Teff = 4.8 °C). The concentration of CO2 and O2 in the headspace of the package, volatile compounds and of the microbial population were monitored during storage. The kinetic parameters for bacterial growth were estimated at 0–10 °C using the Baranyi growth model. The MAP-PAD samples presented significantly lower microbial growth rates and longer lag phases compared to the MAP samples, leading to significant shelf-life extension: 2 days of extension at 2.5 °C and 5 °C, while 50% extension at variable conditions (Teff = 4.8 °C). CO2 emitters in the package improved the chemical freshness (K-values) and volatile compounds (characterizing freshness). The responses of different enzymatic TTI were modeled as the function of enzyme concentration, temperature and storage time. The activation energy (Ea) ranged from 97 to 148 kJ mol−1, allowing the selection of appropriate TTIs for the shelf-life monitoring of each fish product: LP-150U for the MAP and M-25U for the MAP-PAD samples. The validation experiment at Teff = 4.8 °C confirmed the applicability of Arrhenius-type models, as well as the use of TTIs as effective chill chain management tools during distribution and storage.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The study investigated the effect of active modified atmosphere packaging (20% CO2–60% N2–20% O2) with CO2 emitters (MAP-PAD) and conventional MAP (MAP) on the quality and shelf-life of gilthead seabream fillets during chill storage, while the most appropriate enzymatic Time Temperature Integrators (TTI) were selected for monitoring their shelf-life at isothermal and variable temperature storage conditions (Teff = 4.8 °C). The concentration of CO2 and O2 in the headspace of the package, volatile compounds and of the microbial population were monitored during storage. The kinetic parameters for bacterial growth were estimated at 0–10 °C using the Baranyi growth model. The MAP-PAD samples presented significantly lower microbial growth rates and longer lag phases compared to the MAP samples, leading to significant shelf-life extension: 2 days of extension at 2.5 °C and 5 °C, while 50% extension at variable conditions (Teff = 4.8 °C). CO2 emitters in the package improved the chemical freshness (K-values) and volatile compounds (characterizing freshness). The responses of different enzymatic TTI were modeled as the function of enzyme concentration, temperature and storage time. The activation energy (Ea) ranged from 97 to 148 kJ mol−1, allowing the selection of appropriate TTIs for the shelf-life monitoring of each fish product: LP-150U for the MAP and M-25U for the MAP-PAD samples. The validation experiment at Teff = 4.8 °C confirmed the applicability of Arrhenius-type models, as well as the use of TTIs as effective chill chain management tools during distribution and storage.
2021
Rigos, George; Kogiannou, Dimitra; Vasilaki, Antigoni; Kotsiri, Mado
Evaluation of Praziquantel Efficacy against Zeuxapta seriolae Infections in Greater Amberjack, Seriola dumerili Journal Article
In: Applied Sciences, vol. 11, no. 10, pp. 4656, 2021, ISSN: 2076-3417.
@article{rigos_evaluation_2021,
title = {Evaluation of Praziquantel Efficacy against Zeuxapta seriolae Infections in Greater Amberjack, Seriola dumerili},
author = {George Rigos and Dimitra Kogiannou and Antigoni Vasilaki and Mado Kotsiri},
url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/06/2021-Rigos-ApllSciences-41l.pdf
https://www.mdpi.com/2076-3417/11/10/4656},
doi = {10.3390/app11104656},
issn = {2076-3417},
year = {2021},
date = {2021-05-01},
urldate = {2021-06-24},
journal = {Applied Sciences},
volume = {11},
number = {10},
pages = {4656},
abstract = {The aim of this study was to evaluate the efficacy of dietary praziquantel (PZQ), a promising fish anthelminthic, against Zeuxapta seriolae, a lethal ectoparasite of greater amberjack (Seriola dumerili Risso) farmed in the Mediterranean and elsewhere. The trial was carried out in small cages (3 m3) in situ, harbouring fish (125 ± 14 g) naturally infected with Z. seriolae, at 25 ± 1 °C water temperature. Praziquantel-medicated diet (150 mg PZQ/kg fish) was delivered for three days against a control group. Measurement of both adults and oncomiracidia of Z. seriolae in sampled gill arches revealed significant differences between the tested groups (PZQ: 19.3 ± 9.8 vs. Control: 3.8 ± 6.3). Considering an estimated efficacy of 80.4% in the medicated fish, the present study indicates that oral PZQ treatments can confront Z. seriolae infections considerably in farmed greater amberjack and, perhaps, replace the commonly used hydrogen peroxidase baths.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The aim of this study was to evaluate the efficacy of dietary praziquantel (PZQ), a promising fish anthelminthic, against Zeuxapta seriolae, a lethal ectoparasite of greater amberjack (Seriola dumerili Risso) farmed in the Mediterranean and elsewhere. The trial was carried out in small cages (3 m3) in situ, harbouring fish (125 ± 14 g) naturally infected with Z. seriolae, at 25 ± 1 °C water temperature. Praziquantel-medicated diet (150 mg PZQ/kg fish) was delivered for three days against a control group. Measurement of both adults and oncomiracidia of Z. seriolae in sampled gill arches revealed significant differences between the tested groups (PZQ: 19.3 ± 9.8 vs. Control: 3.8 ± 6.3). Considering an estimated efficacy of 80.4% in the medicated fish, the present study indicates that oral PZQ treatments can confront Z. seriolae infections considerably in farmed greater amberjack and, perhaps, replace the commonly used hydrogen peroxidase baths.