This user account status is Approved
2022
Mastoraki, Maria; Katsika, Lydia; Enes, Paula; Guerreiro, Inês; Kotzamanis, Yannis P; Gasco, Laura; Chatzifotis, Stavros; Antonopoulou, Efthimia
In: Aquaculture, vol. 561, pp. 738674, 2022, ISSN: 00448486.
@article{mastoraki_insect_2022,
title = {Insect meals in feeds for juvenile gilthead seabream (Sparus aurata): Effects on growth, blood chemistry, hepatic metabolic enzymes, body composition and nutrient utilization},
author = {Maria Mastoraki and Lydia Katsika and Paula Enes and Inês Guerreiro and Yannis P Kotzamanis and Laura Gasco and Stavros Chatzifotis and Efthimia Antonopoulou},
url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/10/2022-Mastoraki-AQUApre-print-70.pdf
https://linkinghub.elsevier.com/retrieve/pii/S0044848622007918},
doi = {10.1016/j.aquaculture.2022.738674},
issn = {00448486},
year = {2022},
date = {2022-10-14},
urldate = {2022-10-14},
journal = {Aquaculture},
volume = {561},
pages = {738674},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Mente, Eleni; Bousdras, Thomas; Feidantsis, Konstantinos; Panteli, Nikolas; Mastoraki, Maria; Kormas, Konstantinos Ar.; Chatzifotis, Stavros; Piccolo, Giovanni; Gasco, Laura; Gai, Francesco; Martin, Samuel A M; Antonopoulou, Efthimia
Tenebrio molitor larvae meal inclusion affects hepatic proteome and apoptosis and/or autophagy of three farmed fish species Journal Article
In: Scientific Reports, vol. 12, no. 1, pp. 121, 2022, ISSN: 2045-2322.
@article{mente_tenebrio_2022,
title = {Tenebrio molitor larvae meal inclusion affects hepatic proteome and apoptosis and/or autophagy of three farmed fish species},
author = {Eleni Mente and Thomas Bousdras and Konstantinos Feidantsis and Nikolas Panteli and Maria Mastoraki and Konstantinos Ar. Kormas and Stavros Chatzifotis and Giovanni Piccolo and Laura Gasco and Francesco Gai and Samuel A M Martin and Efthimia Antonopoulou},
url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/05/2022-Mente-SciRep-23.pdf
https://www.nature.com/articles/s41598-021-03306-8},
doi = {10.1038/s41598-021-03306-8},
issn = {2045-2322},
year = {2022},
date = {2022-04-29},
urldate = {2022-05-09},
journal = {Scientific Reports},
volume = {12},
number = {1},
pages = {121},
abstract = {Abstract
Herein, the effect of dietary inclusion of insect (
Tenebrio molitor
) meal on hepatic pathways of apoptosis and autophagy in three farmed fish species, gilthead seabream (
Sparus aurata
), European seabass (
Dicentrarchus labrax
) and rainbow trout (
Oncorhynchus mykiss
), fed diets at 25%, 50% and 60% insect meal inclusion levels respectively, was investigated. Hepatic proteome was examined by liver protein profiles from the three fish species, obtained by two-dimensional gel electrophoresis. Although cellular stress was evident in the three teleost species following insect meal, inclusion by
T. molitor
,
D. labrax
and
O. mykiss
suppressed apoptosis through induction of hepatic autophagy, while in
S. aurata
both cellular procedures were activated. Protein abundance showed that a total of 30, 81 and 74 spots were altered significantly in seabream, European seabass and rainbow trout, respectively. Insect meal inclusion resulted in individual protein abundance changes, with less number of proteins altered in gilthead seabream compared to European seabass and rainbow trout. This is the first study demonstrating that insect meal in fish diets is causing changes in liver protein abundances. However, a species-specific response both in the above mentioned bioindicators, indicates the need to strategically manage fish meal replacement in fish diets per species.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Abstract
Herein, the effect of dietary inclusion of insect (
Tenebrio molitor
) meal on hepatic pathways of apoptosis and autophagy in three farmed fish species, gilthead seabream (
Sparus aurata
), European seabass (
Dicentrarchus labrax
) and rainbow trout (
Oncorhynchus mykiss
), fed diets at 25%, 50% and 60% insect meal inclusion levels respectively, was investigated. Hepatic proteome was examined by liver protein profiles from the three fish species, obtained by two-dimensional gel electrophoresis. Although cellular stress was evident in the three teleost species following insect meal, inclusion by
T. molitor
,
D. labrax
and
O. mykiss
suppressed apoptosis through induction of hepatic autophagy, while in
S. aurata
both cellular procedures were activated. Protein abundance showed that a total of 30, 81 and 74 spots were altered significantly in seabream, European seabass and rainbow trout, respectively. Insect meal inclusion resulted in individual protein abundance changes, with less number of proteins altered in gilthead seabream compared to European seabass and rainbow trout. This is the first study demonstrating that insect meal in fish diets is causing changes in liver protein abundances. However, a species-specific response both in the above mentioned bioindicators, indicates the need to strategically manage fish meal replacement in fish diets per species.
Herein, the effect of dietary inclusion of insect (
Tenebrio molitor
) meal on hepatic pathways of apoptosis and autophagy in three farmed fish species, gilthead seabream (
Sparus aurata
), European seabass (
Dicentrarchus labrax
) and rainbow trout (
Oncorhynchus mykiss
), fed diets at 25%, 50% and 60% insect meal inclusion levels respectively, was investigated. Hepatic proteome was examined by liver protein profiles from the three fish species, obtained by two-dimensional gel electrophoresis. Although cellular stress was evident in the three teleost species following insect meal, inclusion by
T. molitor
,
D. labrax
and
O. mykiss
suppressed apoptosis through induction of hepatic autophagy, while in
S. aurata
both cellular procedures were activated. Protein abundance showed that a total of 30, 81 and 74 spots were altered significantly in seabream, European seabass and rainbow trout, respectively. Insect meal inclusion resulted in individual protein abundance changes, with less number of proteins altered in gilthead seabream compared to European seabass and rainbow trout. This is the first study demonstrating that insect meal in fish diets is causing changes in liver protein abundances. However, a species-specific response both in the above mentioned bioindicators, indicates the need to strategically manage fish meal replacement in fish diets per species.
Antonopoulou, Efthimia; Panteli, Nikolas; Feidantsis, Kostantinos; Mastoraki, Maria; Koutsogeorgiou, Eleni; Grivaki, Eirini; Papagrigoriou, Theodora; Christias, Spyros; Chatzifotis, Stavros; Lazari, Diamanto; Andreadis, Stefanos; Krigas, Nikos
In: Antioxidants, vol. 11, no. 9, pp. 1840, 2022, ISSN: 2076-3921.
@article{antonopoulou_carob_2022,
title = {Carob (Ceratonia siliqua) as Functional Feed Is Beneficial in Yellow Mealworm (Tenebrio molitor) Rearing: Evidence from Growth, Antioxidant Status and Cellular Responses},
author = {Efthimia Antonopoulou and Nikolas Panteli and Kostantinos Feidantsis and Maria Mastoraki and Eleni Koutsogeorgiou and Eirini Grivaki and Theodora Papagrigoriou and Spyros Christias and Stavros Chatzifotis and Diamanto Lazari and Stefanos Andreadis and Nikos Krigas},
url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/10/2022-Antonopoulou-Antioxidants-69-1.pdf
https://www.mdpi.com/2076-3921/11/9/1840},
doi = {10.3390/antiox11091840},
issn = {2076-3921},
year = {2022},
date = {2022-01-01},
urldate = {2022-10-12},
journal = {Antioxidants},
volume = {11},
number = {9},
pages = {1840},
abstract = {In terms of sustainability and circular economy, agricultural by-products may be efficiently reused in insects’ rearing for high-quality protein sources in human diet and animal feeds. The present study aimed to explore whether the utilization of carob pods as feeding substrate may beneficially affect Tenebrio molitor’s growth, nutritional value, antioxidant status and cellular responses. Increasing levels of milled whole carob pods (0, 25, 50, 75, 100%) were used as alternative wheat bran (control) substrates for yellow mealworm rearing, while growth performance, proximate composition, total phenolic content, antioxidant enzyme activity and the expression of stress- and apoptotic-related proteins were evaluated in larvae. The results showed that carob pods’ content up to 75% did not significantly differentiate larvae weight, development time and total dry matter. Larvae total phenolic content and antioxidant activity exhibited a significant increase at 75% content. Although the antioxidant enzymes’ activity decreased at both 25 and 50% levels, higher carob content levels (75 and 100%) resulted in no significant changes compared to the control. Carob pods led to decreased apoptotic indicators and the low expression of most stress-related proteins compared to the control. The present findings demonstrate that carob pods and their antioxidant properties exert beneficial effects on T. molitor’s rearing and nutritional status, although 100% carob content may impact adversely the larvae due to the high amounts of carob tannins.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In terms of sustainability and circular economy, agricultural by-products may be efficiently reused in insects’ rearing for high-quality protein sources in human diet and animal feeds. The present study aimed to explore whether the utilization of carob pods as feeding substrate may beneficially affect Tenebrio molitor’s growth, nutritional value, antioxidant status and cellular responses. Increasing levels of milled whole carob pods (0, 25, 50, 75, 100%) were used as alternative wheat bran (control) substrates for yellow mealworm rearing, while growth performance, proximate composition, total phenolic content, antioxidant enzyme activity and the expression of stress- and apoptotic-related proteins were evaluated in larvae. The results showed that carob pods’ content up to 75% did not significantly differentiate larvae weight, development time and total dry matter. Larvae total phenolic content and antioxidant activity exhibited a significant increase at 75% content. Although the antioxidant enzymes’ activity decreased at both 25 and 50% levels, higher carob content levels (75 and 100%) resulted in no significant changes compared to the control. Carob pods led to decreased apoptotic indicators and the low expression of most stress-related proteins compared to the control. The present findings demonstrate that carob pods and their antioxidant properties exert beneficial effects on T. molitor’s rearing and nutritional status, although 100% carob content may impact adversely the larvae due to the high amounts of carob tannins.
2021
Andreadis, Stefanos S; Panteli, Nikolas; Mastoraki, Maria; Rizou, Eleftheria; Stefanou, Vassilia; Tzentilasvili, Sofia; Sarrou, Eirini; Chatzifotis, Stavros; Krigas, Nikos; Antonopoulou, Efthimia
In: Antioxidants, vol. 11, no. 1, pp. 68, 2021, ISSN: 2076-3921.
@article{andreadis_towards_2021,
title = {Towards Functional Insect Feeds: Agri-Food By-Products Enriched with Post-Distillation Residues of Medicinal Aromatic Plants in Tenebrio molitor (Coleoptera: Tenebrionidae) Breeding},
author = {Stefanos S Andreadis and Nikolas Panteli and Maria Mastoraki and Eleftheria Rizou and Vassilia Stefanou and Sofia Tzentilasvili and Eirini Sarrou and Stavros Chatzifotis and Nikos Krigas and Efthimia Antonopoulou},
url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/05/2022-Αndreadis-antioxidants-22.pdf
https://www.mdpi.com/2076-3921/11/1/68},
doi = {10.3390/antiox11010068},
issn = {2076-3921},
year = {2021},
date = {2021-12-01},
urldate = {2022-05-09},
journal = {Antioxidants},
volume = {11},
number = {1},
pages = {68},
abstract = {Sustainability, circular economy and alternative production systems are urgent imperatives for humanity and animal husbandry. Unless wasted, agri-food by-products can offer a promising source of high value. We evaluated the effect of rice bran (RB), corncob (CC), potato peels (PP), solid biogas residues (BR), and olive-oil processing residuals (OR), as alternative substrates to wheat bran (WB as control), on the growth and nutritional value of Tenebrio molitor during its breeding for animal feeds and/or human consumption. Innovation-wise, we further investigated the substrate supplementation (0, 10, 20%) with post-distillation residues of Mediterranean aromatic-medicinal plants (MAPs: lavender, Greek oregano, rosemary, olive; 1:1:1:1 ratio). Tenebrio molitor larvae (TML) were reared in all the studied substrates, and TML and diets’ proximate and fatty acid compositions as well as total phenol and flavonoid content and antioxidant potential were assessed using standard procedures. After statistical analysis of correlations, we observed that CC promoted oviposition and progeny survival; larval weight and dry matter were positively affected mainly by dietary energy and fat content; number of TML and/or larval weight increased using 10% MAPs inclusion in WB, RB and OR or RB, OR, BR and PP, respectively, which did not affect protein content; TML fatty acid composition decreased the content of saturated ones and increased that of mono-unsaturated ones; MAPs residues had an apparent favorable impact on total phenolic content and antioxidant activity of each substrate, with RB displaying the highest capacity and content. These findings indicate that alternative substrates can be exploited and their enrichment with natural phenolics is able to influence T. molitor growth, offering highly beneficial and nutritional value.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Sustainability, circular economy and alternative production systems are urgent imperatives for humanity and animal husbandry. Unless wasted, agri-food by-products can offer a promising source of high value. We evaluated the effect of rice bran (RB), corncob (CC), potato peels (PP), solid biogas residues (BR), and olive-oil processing residuals (OR), as alternative substrates to wheat bran (WB as control), on the growth and nutritional value of Tenebrio molitor during its breeding for animal feeds and/or human consumption. Innovation-wise, we further investigated the substrate supplementation (0, 10, 20%) with post-distillation residues of Mediterranean aromatic-medicinal plants (MAPs: lavender, Greek oregano, rosemary, olive; 1:1:1:1 ratio). Tenebrio molitor larvae (TML) were reared in all the studied substrates, and TML and diets’ proximate and fatty acid compositions as well as total phenol and flavonoid content and antioxidant potential were assessed using standard procedures. After statistical analysis of correlations, we observed that CC promoted oviposition and progeny survival; larval weight and dry matter were positively affected mainly by dietary energy and fat content; number of TML and/or larval weight increased using 10% MAPs inclusion in WB, RB and OR or RB, OR, BR and PP, respectively, which did not affect protein content; TML fatty acid composition decreased the content of saturated ones and increased that of mono-unsaturated ones; MAPs residues had an apparent favorable impact on total phenolic content and antioxidant activity of each substrate, with RB displaying the highest capacity and content. These findings indicate that alternative substrates can be exploited and their enrichment with natural phenolics is able to influence T. molitor growth, offering highly beneficial and nutritional value.
Katsika, Lydia; Tasbozan, Oguz; Mastoraki, Maria; Karapanagiotis, Stelios; Zalamitsou, Chrysa; Feidantsis, Konstantinos; Antonopoulou, Efthimia; Chatzifotis, Stavros
In: Aquaculture Research, vol. 52, no. 11, pp. 5760–5776, 2021, ISSN: 1355-557X, 1365-2109.
@article{katsika_effects_2021,
title = {Effects of fish oil substitution by hazelnut oil on growth performance, whole‐body fatty acid composition and enzymes of intermediary metabolism of juvenile meagre ( textitArgyrosomus regius Asso, 1801)},
author = {Lydia Katsika and Oguz Tasbozan and Maria Mastoraki and Stelios Karapanagiotis and Chrysa Zalamitsou and Konstantinos Feidantsis and Efthimia Antonopoulou and Stavros Chatzifotis},
url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/12/2021-Katsika-ARE-pre-print-78.pdf
https://onlinelibrary.wiley.com/doi/10.1111/are.15452},
doi = {10.1111/are.15452},
issn = {1355-557X, 1365-2109},
year = {2021},
date = {2021-11-01},
urldate = {2021-12-01},
journal = {Aquaculture Research},
volume = {52},
number = {11},
pages = {5760--5776},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Panteli, Nikolas; Mastoraki, Maria; Lazarina, Maria; Chatzifotis, Stavros; Mente, Eleni; Kormas, Konstantinos Ar.; Antonopoulou, Efthimia
Configuration of Gut Microbiota Structure and Potential Functionality in Two Teleosts under the Influence of Dietary Insect Meals Journal Article
In: Microorganisms, vol. 9, no. 4, pp. 699, 2021, ISSN: 2076-2607, (AQUA).
@article{panteli_configuration_2021,
title = {Configuration of Gut Microbiota Structure and Potential Functionality in Two Teleosts under the Influence of Dietary Insect Meals},
author = {Nikolas Panteli and Maria Mastoraki and Maria Lazarina and Stavros Chatzifotis and Eleni Mente and Konstantinos Ar. Kormas and Efthimia Antonopoulou},
url = {https://www.mdpi.com/2076-2607/9/4/699},
doi = {10.3390/microorganisms9040699},
issn = {2076-2607},
year = {2021},
date = {2021-01-01},
urldate = {2022-01-17},
journal = {Microorganisms},
volume = {9},
number = {4},
pages = {699},
abstract = {Insect meals are considered promising, eco-friendly, alternative ingredients for aquafeed. Considering the dietary influence on establishment of functioning gut microbiota, the effect of the insect meal diets on the microbial ecology should be addressed. The present study assessed diet- and species-specific shifts in gut resident bacterial communities of juvenile reared Dicentrarchus labrax and Sparus aurata in response to three experimental diets with insect meals from three insects (Hermetia illucens, Tenebrio molitor, Musca domestica), using high-throughput Illumina sequencing of the V3–V4 region of the 16S rRNA gene. The dominant phyla were Firmicutes, Proteobacteria and Actinobacteria in all dietary treatments. Anaerococcus sp., Cutibacterium sp. and Pseudomonas sp. in D. labrax, and Staphylococcus sp., Hafnia sp. and Aeromonas sp. in S. aurata were the most enriched shared species, following insect-meal inclusion. Network analysis of the dietary treatments highlighted diet-induced changes in the microbial community assemblies and revealed unique and shared microbe-to-microbe interactions. PICRUSt-predicted Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were significantly differentiated, including genes associated with metabolic pathways. The present findings strengthen the importance of diet in microbiota configuration and underline that different insects as fish feed ingredients elicit species-specific differential responses of structural and functional dynamics in gut microbial communities.},
note = {AQUA},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Insect meals are considered promising, eco-friendly, alternative ingredients for aquafeed. Considering the dietary influence on establishment of functioning gut microbiota, the effect of the insect meal diets on the microbial ecology should be addressed. The present study assessed diet- and species-specific shifts in gut resident bacterial communities of juvenile reared Dicentrarchus labrax and Sparus aurata in response to three experimental diets with insect meals from three insects (Hermetia illucens, Tenebrio molitor, Musca domestica), using high-throughput Illumina sequencing of the V3–V4 region of the 16S rRNA gene. The dominant phyla were Firmicutes, Proteobacteria and Actinobacteria in all dietary treatments. Anaerococcus sp., Cutibacterium sp. and Pseudomonas sp. in D. labrax, and Staphylococcus sp., Hafnia sp. and Aeromonas sp. in S. aurata were the most enriched shared species, following insect-meal inclusion. Network analysis of the dietary treatments highlighted diet-induced changes in the microbial community assemblies and revealed unique and shared microbe-to-microbe interactions. PICRUSt-predicted Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were significantly differentiated, including genes associated with metabolic pathways. The present findings strengthen the importance of diet in microbiota configuration and underline that different insects as fish feed ingredients elicit species-specific differential responses of structural and functional dynamics in gut microbial communities.
2020
Mastoraki, Maria; Ferrándiz, Paula Mollá; Vardali, Sofia C; Kontodimas, Demetrius C; Kotzamanis, Yannis P; Gasco, Laura; Chatzifotis, Stavros; Antonopoulou, Efthimia
In: Aquaculture, vol. 528, pp. 735511, 2020, ISSN: 00448486.
@article{mastoraki_comparative_2020,
title = {A comparative study on the effect of fish meal substitution with three different insect meals on growth, body composition and metabolism of European sea bass (Dicentrarchus labrax L.)},
author = {Maria Mastoraki and Paula Mollá Ferrándiz and Sofia C Vardali and Demetrius C Kontodimas and Yannis P Kotzamanis and Laura Gasco and Stavros Chatzifotis and Efthimia Antonopoulou},
url = {https://linkinghub.elsevier.com/retrieve/pii/S004484862030065X},
doi = {10.1016/j.aquaculture.2020.735511},
issn = {00448486},
year = {2020},
date = {2020-11-01},
urldate = {2020-07-06},
journal = {Aquaculture},
volume = {528},
pages = {735511},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Mastoraki, Maria; Vlahos, Nikolaos; Patsea, Efstathia; Chatzifotis, Stavros; Mente, Eleni; Antonopoulou, Efthimia
In: Aquaculture Research, vol. 51, no. 9, pp. 3551–3562, 2020, ISSN: 1355-557X, 1365-2109.
@article{mastoraki_effect_2020,
title = {The effect of insect meal as a feed ingredient on survival, growth, and metabolic and antioxidant response of juvenile prawn textitPalaemon adspersus (Rathke, 1837)},
author = {Maria Mastoraki and Nikolaos Vlahos and Efstathia Patsea and Stavros Chatzifotis and Eleni Mente and Efthimia Antonopoulou},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/are.14692},
doi = {10.1111/are.14692},
issn = {1355-557X, 1365-2109},
year = {2020},
date = {2020-09-01},
urldate = {2020-08-31},
journal = {Aquaculture Research},
volume = {51},
number = {9},
pages = {3551--3562},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
2018
Sofoulaki, K; Kalantzi, I; Machias, A; Mastoraki, M; Chatzifotis, S; Mylona, K; Pergantis, S A; Tsapakis, M
Metals and elements in sardine and anchovy: Species specific differences and correlations with proximate composition and size Journal Article
In: Science of the Total Environment, vol. 645, pp. 329–338, 2018, ISSN: 00489697, (Publisher: Elsevier B.V.).
@article{sofoulaki_metals_2018,
title = {Metals and elements in sardine and anchovy: Species specific differences and correlations with proximate composition and size},
author = {K Sofoulaki and I Kalantzi and A Machias and M Mastoraki and S Chatzifotis and K Mylona and S A Pergantis and M Tsapakis},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85049968790&doi=10.1016%2fj.scitotenv.2018.07.133&partnerID=40&md5=0d484f76540527fffb262d6ab87ad0ff},
doi = {10.1016/j.scitotenv.2018.07.133},
issn = {00489697},
year = {2018},
date = {2018-01-01},
journal = {Science of the Total Environment},
volume = {645},
pages = {329--338},
abstract = {Species – specific differences in the levels of 26 metals and elements in sardine and anchovy are investigated and the factors of proximate composition (proteins, lipids, ash) and body size (length, weight) that may affect the metal and elemental concentrations in fish are explored. Statistical analysis revealed that levels of metals and elements in fish seem to strongly depend on species. Significantly higher levels of most of the metals and elements studied have been observed in anchovy compared to sardine at each of six different sites. The observed species-specific differences in metal content could be attributed to different proximate composition and size among other factors. The significant (positive or negative) correlations found between lipids, proteins, ash, weight, length and metals or elements, suggest that higher levels of most of the metals and elements studied can be associated with lower lipid content, greater protein and ash content and smaller size. © 2018 Elsevier B.V.},
note = {Publisher: Elsevier B.V.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Species – specific differences in the levels of 26 metals and elements in sardine and anchovy are investigated and the factors of proximate composition (proteins, lipids, ash) and body size (length, weight) that may affect the metal and elemental concentrations in fish are explored. Statistical analysis revealed that levels of metals and elements in fish seem to strongly depend on species. Significantly higher levels of most of the metals and elements studied have been observed in anchovy compared to sardine at each of six different sites. The observed species-specific differences in metal content could be attributed to different proximate composition and size among other factors. The significant (positive or negative) correlations found between lipids, proteins, ash, weight, length and metals or elements, suggest that higher levels of most of the metals and elements studied can be associated with lower lipid content, greater protein and ash content and smaller size. © 2018 Elsevier B.V.