2023 |
Vasilaki, A; Mente, E; Fountoulaki, E; Henry, M; Nikoloudaki, C; Berillis, P; Kousoulaki, K; Nengas, I Fishmeal, plant protein, and fish oil substitution with single-cell ingredients in organic feeds for European sea bass (Dicentrarchus labrax) Journal Article Frontiers in Physiology, 14 , pp. 1199497, 2023, ISSN: 1664-042X. @article{vasilaki_fishmeal_2023, title = {Fishmeal, plant protein, and fish oil substitution with single-cell ingredients in organic feeds for European sea bass (Dicentrarchus labrax)}, author = {A Vasilaki and E Mente and E Fountoulaki and M Henry and C Nikoloudaki and P Berillis and K Kousoulaki and I Nengas}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/06/2023-Vasilaki-Frontiers-Physio-28.pdf https://www.frontiersin.org/articles/10.3389/fphys.2023.1199497/full}, doi = {10.3389/fphys.2023.1199497}, issn = {1664-042X}, year = {2023}, date = {2023-06-06}, urldate = {2023-06-12}, journal = {Frontiers in Physiology}, volume = {14}, pages = {1199497}, abstract = {Single-cell ingredients (SCI) are considered promising nutrient sources which are produced using environmentally friendly biotechnological processes. The aim of the current study was to evaluate the replacement of fishmeal, plant protein sources, and fish oil with SCI in organic feeds for European sea bass ( Dicentrarchus labrax ). Bacterial protein, yeast protein, and microalgae were used to replace fishmeal trimmings, soya bean meal, and fish oil from trimmings. Triplicate groups (30 fish per replicate) of European sea bass (14.4 ± 2.4 g) were fed the experimental diets for 71 days. The results showed that the incorporation of SCI at all levels of inclusion significantly enhanced nutrient digestibility. Additionally, growth performance parameters were not affected by SCI inclusion, exhibiting similar or improved values. Moreover, a tendency for improved anterior and posterior gut structure was observed and a significant increase of lysozyme activity at the two highest inclusion levels of SCI was determined. Overall, the results showed that the inclusion of SCI at 15% (bacterial: yeast: algae—9.4: 4.7: 1) is possible without compromising any of the parameters evaluated. According to these findings, a higher substitution of fishmeal trimmings, plant protein sources, and fish oil from trimmings with SCI in organic diets for European sea bass ( D. labrax ) can be further evaluated in future studies.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Single-cell ingredients (SCI) are considered promising nutrient sources which are produced using environmentally friendly biotechnological processes. The aim of the current study was to evaluate the replacement of fishmeal, plant protein sources, and fish oil with SCI in organic feeds for European sea bass ( Dicentrarchus labrax ). Bacterial protein, yeast protein, and microalgae were used to replace fishmeal trimmings, soya bean meal, and fish oil from trimmings. Triplicate groups (30 fish per replicate) of European sea bass (14.4 ± 2.4 g) were fed the experimental diets for 71 days. The results showed that the incorporation of SCI at all levels of inclusion significantly enhanced nutrient digestibility. Additionally, growth performance parameters were not affected by SCI inclusion, exhibiting similar or improved values. Moreover, a tendency for improved anterior and posterior gut structure was observed and a significant increase of lysozyme activity at the two highest inclusion levels of SCI was determined. Overall, the results showed that the inclusion of SCI at 15% (bacterial: yeast: algae—9.4: 4.7: 1) is possible without compromising any of the parameters evaluated. According to these findings, a higher substitution of fishmeal trimmings, plant protein sources, and fish oil from trimmings with SCI in organic diets for European sea bass ( D. labrax ) can be further evaluated in future studies. |
2022 |
Henry, M A; Golomazou, E; Asimaki, A; Psofakis, P; Fountoulaki, E; Mente, E; Rumbos, C I; Athanassiou, C G; Karapanagiotidis, I T Aquaculture Reports, 27 , pp. 101347, 2022, ISSN: 23525134. @article{henry_partial_2022, title = {Partial dietary fishmeal replacement with full-fat or defatted superworm (Zophobas morio) larvae meals modulates the innate immune system of gilthead seabream, Sparus aurata}, author = {M A Henry and E Golomazou and A Asimaki and P Psofakis and E Fountoulaki and E Mente and C I Rumbos and C G Athanassiou and I T Karapanagiotidis}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/10/2022-Henry-AquaRepo-66.pdf https://linkinghub.elsevier.com/retrieve/pii/S235251342200343X}, doi = {10.1016/j.aqrep.2022.101347}, issn = {23525134}, year = {2022}, date = {2022-10-19}, urldate = {2022-10-19}, journal = {Aquaculture Reports}, volume = {27}, pages = {101347}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Michail, George; Berillis, Panagiotis; Nakas, Christos; Henry, Morgane; Mente, Eleni Haematology reference values for textitDicentrarchus labrax and textitSparus aurata : A systematic review and meta‐analysis Journal Article Journal of Fish Diseases, 45 (10), pp. 1549–1570, 2022, ISSN: 0140-7775, 1365-2761. @article{michail_haematology_2022, title = {Haematology reference values for textitDicentrarchus labrax and textitSparus aurata : A systematic review and meta‐analysis}, author = {George Michail and Panagiotis Berillis and Christos Nakas and Morgane Henry and Eleni Mente}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/09/2022-Michail-FD-61-preprint.pdf https://onlinelibrary.wiley.com/doi/10.1111/jfd.13680}, doi = {10.1111/jfd.13680}, issn = {0140-7775, 1365-2761}, year = {2022}, date = {2022-01-01}, urldate = {2022-09-26}, journal = {Journal of Fish Diseases}, volume = {45}, number = {10}, pages = {1549--1570}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2021 |
Gasco, L; Józefiak, A; Henry, M Beyond the protein concept: health aspects of using edible insects on animals Journal Article 7 (5), pp. 715–741, 2021, ISSN: 2352-4588. @article{gasco_beyond_2021, title = {Beyond the protein concept: health aspects of using edible insects on animals}, author = {L Gasco and A Józefiak and M Henry}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/09/2021-Gasco-Henry-JIFF-67.pdf https://www.wageningenacademic.com/doi/10.3920/JIFF2020.0077}, doi = {10.3920/JIFF2020.0077}, issn = {2352-4588}, year = {2021}, date = {2021-08-13}, urldate = {2021-09-22}, volume = {7}, number = {5}, pages = {715--741}, abstract = {There is an increasing interest in the use of insects in animal feed since they contain high proteins levels, lipids, vitamins and minerals. In particular, insect-derived proteins are seen as one of the potential solution to face the increasing protein shortage and are able to fully substitute soybean meal or fishmeal in aquaculture or livestock feeds. However, beside their interesting nutritional composition, insects are also rich in bioactive compounds such as chitin, antimicrobial peptides or specific fatty acids with immunostimulating, antimicrobial and/or anti-inflammatory properties able to sustain animal health, increase their resistance to diseases. Further studies will also have to investigate whether insects share similarities with bacterial or parasitical pathogens and may act as immunostimulants. These recent findings may launch insects beyond the protein concept into healthy animal feeds. This review presents the effects of insects and their bioactive compounds on fish and crustaceans, poultry, pigs and rabbits immune system, gut health, microbiota and resistance to diseases.}, keywords = {}, pubstate = {published}, tppubtype = {article} } There is an increasing interest in the use of insects in animal feed since they contain high proteins levels, lipids, vitamins and minerals. In particular, insect-derived proteins are seen as one of the potential solution to face the increasing protein shortage and are able to fully substitute soybean meal or fishmeal in aquaculture or livestock feeds. However, beside their interesting nutritional composition, insects are also rich in bioactive compounds such as chitin, antimicrobial peptides or specific fatty acids with immunostimulating, antimicrobial and/or anti-inflammatory properties able to sustain animal health, increase their resistance to diseases. Further studies will also have to investigate whether insects share similarities with bacterial or parasitical pathogens and may act as immunostimulants. These recent findings may launch insects beyond the protein concept into healthy animal feeds. This review presents the effects of insects and their bioactive compounds on fish and crustaceans, poultry, pigs and rabbits immune system, gut health, microbiota and resistance to diseases. |
2020 |
Henry, Morgane A; Kokou, Fotini; Palenzuela, Oswaldo; Pyrenis, George; Rigos, George Folia Parasitologica, 67 , 2020, ISSN: 00155683, 18036465. @article{henry_experimental_2020, title = {Experimental infection model with the bivalvulid Enteromyxum leei (Myxidiidae) in the sharpsnout seabream, Diplodus puntazzo (Sparidae), and evaluation of the antiparasitic efficacy of a functional diet}, author = {Morgane A Henry and Fotini Kokou and Oswaldo Palenzuela and George Pyrenis and George Rigos}, url = {http://folia.paru.cas.cz/doi/10.14411/fp.2020.024.html}, doi = {10.14411/fp.2020.024}, issn = {00155683, 18036465}, year = {2020}, date = {2020-09-01}, urldate = {2020-12-08}, journal = {Folia Parasitologica}, volume = {67}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Kokou, Fotini; Bastias, Roberto; Kokkari, Konstantina; Katharios, Pantelis; Cotou, Efthimia; Seimenis, Nikos; Vasilakis, Manolis; Papandroulakis, Nikos; Henry, Morgane; Rigos, George Surplus of dietary micronutrients promotes antioxidant defense and improves fin erosions in European seabass (Dicentrarchus labrax) fry Journal Article Aquaculture, 523 , pp. 735224, 2020, ISSN: 0044-8486. @article{kokou_surplus_2020, title = {Surplus of dietary micronutrients promotes antioxidant defense and improves fin erosions in European seabass (Dicentrarchus labrax) fry}, author = {Fotini Kokou and Roberto Bastias and Konstantina Kokkari and Pantelis Katharios and Efthimia Cotou and Nikos Seimenis and Manolis Vasilakis and Nikos Papandroulakis and Morgane Henry and George Rigos}, url = {http://www.sciencedirect.com/science/article/pii/S0044848619330868}, doi = {https://doi.org/10.1016/j.aquaculture.2020.735224}, issn = {0044-8486}, year = {2020}, date = {2020-01-01}, journal = {Aquaculture}, volume = {523}, pages = {735224}, abstract = {Dietary micronutrient supplementation can serve as skin health promotor to prevent from natural infections and can be applied to decrease the use of antibacterial agents and their impact on the environment. Such supplementation has indicated anti-inflammatory and antioxidant properties in farmed fish. In this study, dietary supplementation of zinc, selenium, ascorbic acid and niacin, at levels 2 to 3-fold higher than recommended, was evaluated as a measure to promote skin health and antioxidant defense in European seabass, Dicentrarchus labrax, fry. For this purpose, fish survival, growth performance, fin and skin erosions as well as whole-fish antioxidant enzyme activity (catalase, selenium (Se)-independent and dependent glutathione peroxidase) were assessed. Moreover, bacterial load in the rearing water and skin mucus were evaluated to assess the potential of the dietary micronutrient mixture as a preventive from natural infections. A group fed with a diet including these micronutrients at recommended levels served as a control. Surplus of these micronutrients significantly reduced the percentage of fish with eroded fins, while also induced the activity of catalase and Se-dependent glutathione peroxidase. Fish growth and bacterial loads in the water and fish skin mucus were not affected, whereas no skin lesions were observed. Overall, a mineral- and vitamin-supplemented diet at levels higher than recommended seems to act as a promotor of skin health and prevent from fin erosions, potentially through an increase in the antioxidant defense system.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dietary micronutrient supplementation can serve as skin health promotor to prevent from natural infections and can be applied to decrease the use of antibacterial agents and their impact on the environment. Such supplementation has indicated anti-inflammatory and antioxidant properties in farmed fish. In this study, dietary supplementation of zinc, selenium, ascorbic acid and niacin, at levels 2 to 3-fold higher than recommended, was evaluated as a measure to promote skin health and antioxidant defense in European seabass, Dicentrarchus labrax, fry. For this purpose, fish survival, growth performance, fin and skin erosions as well as whole-fish antioxidant enzyme activity (catalase, selenium (Se)-independent and dependent glutathione peroxidase) were assessed. Moreover, bacterial load in the rearing water and skin mucus were evaluated to assess the potential of the dietary micronutrient mixture as a preventive from natural infections. A group fed with a diet including these micronutrients at recommended levels served as a control. Surplus of these micronutrients significantly reduced the percentage of fish with eroded fins, while also induced the activity of catalase and Se-dependent glutathione peroxidase. Fish growth and bacterial loads in the water and fish skin mucus were not affected, whereas no skin lesions were observed. Overall, a mineral- and vitamin-supplemented diet at levels higher than recommended seems to act as a promotor of skin health and prevent from fin erosions, potentially through an increase in the antioxidant defense system. |
Henry, Morgane A; Fountoulaki, Eleni; Vasilaki, Antigoni; Rigos, George; Kokou, Fotini; Karalazos, Vasileios Aquaculture, 528 , pp. 735479, 2020, ISSN: 0044-8486. @article{henry_dietary_2020, title = {Dietary micronutrient supplementation in low fishmeal based diets for optimum growth and immune status of European sea bass (Dicentrarchus labrax) juveniles}, author = {Morgane A Henry and Eleni Fountoulaki and Antigoni Vasilaki and George Rigos and Fotini Kokou and Vasileios Karalazos}, url = {http://www.sciencedirect.com/science/article/pii/S0044848620312412}, doi = {https://doi.org/10.1016/j.aquaculture.2020.735479}, issn = {0044-8486}, year = {2020}, date = {2020-01-01}, journal = {Aquaculture}, volume = {528}, pages = {735479}, abstract = {The present study aimed to evaluate the optimal dietary micronutrient supplementation of plant-based diets for juvenile European sea bass. A 90-day feeding trial was performed, where fish were fed a high fishmeal (FM) based diet (62% DM; positive control) or low FM diets (12.5% FM and krill meal), supplemented with increasing levels of a micronutrient mixture (Nutrient Package, NP) consisting of vitamins, minerals and other micronutrients. The supplementation levels ranged from 0% (no supplementation, negative control) to 400%, where 100% supplementation level corresponded to the requirement levels recommended for other fish species. Fish growth and feed performance (SGR, DGI, FCR, PER) were significantly impaired at levels NP0 and NP25 compared to the FM control. Body Fe and Zn concentrations were positively affected by the graded levels of the NP, while Mn and Se concentrations remained stable. Growth parameters were particularly correlated to body Fe and Zn, while immune parameters were mainly correlated to body Zn. Significant immunosuppression was evidenced (concerning the respiratory burst activity) when low FM diets were not supplemented with NP. High inclusion levels of the NP showed a negative impact on lysozyme activity. Histological examinations did not reveal any significant effects on gills, gut and liver morphology. At identical NP supplementation level (50%), fish fed low and high FM diets showed similar growth and innate immunity suggesting that long-term feeding with plant-based diets do not significantly affect the fish when adequate minerals and vitamins supplementation is provided. Quadratic regression analysis showed that the inclusion of the NP between 260 and 300% improves growth and immune parameters of fish fed low-FM diets as well as Zn body incorporation. Overall, the present study suggested the positive impact of NP supplementation of PM-based diets on both fish growth and health but future NP could include less Mn, more Fe, closer to levels obtained in fish fed FM (245 mg·kg−1), and Zn at 122 mg·kg−1.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The present study aimed to evaluate the optimal dietary micronutrient supplementation of plant-based diets for juvenile European sea bass. A 90-day feeding trial was performed, where fish were fed a high fishmeal (FM) based diet (62% DM; positive control) or low FM diets (12.5% FM and krill meal), supplemented with increasing levels of a micronutrient mixture (Nutrient Package, NP) consisting of vitamins, minerals and other micronutrients. The supplementation levels ranged from 0% (no supplementation, negative control) to 400%, where 100% supplementation level corresponded to the requirement levels recommended for other fish species. Fish growth and feed performance (SGR, DGI, FCR, PER) were significantly impaired at levels NP0 and NP25 compared to the FM control. Body Fe and Zn concentrations were positively affected by the graded levels of the NP, while Mn and Se concentrations remained stable. Growth parameters were particularly correlated to body Fe and Zn, while immune parameters were mainly correlated to body Zn. Significant immunosuppression was evidenced (concerning the respiratory burst activity) when low FM diets were not supplemented with NP. High inclusion levels of the NP showed a negative impact on lysozyme activity. Histological examinations did not reveal any significant effects on gills, gut and liver morphology. At identical NP supplementation level (50%), fish fed low and high FM diets showed similar growth and innate immunity suggesting that long-term feeding with plant-based diets do not significantly affect the fish when adequate minerals and vitamins supplementation is provided. Quadratic regression analysis showed that the inclusion of the NP between 260 and 300% improves growth and immune parameters of fish fed low-FM diets as well as Zn body incorporation. Overall, the present study suggested the positive impact of NP supplementation of PM-based diets on both fish growth and health but future NP could include less Mn, more Fe, closer to levels obtained in fish fed FM (245 mg·kg−1), and Zn at 122 mg·kg−1. |
2019 |
Motte, Constant; Rios, Alfredo; Lefebvre, Thomas; Do, Hong; Henry, Morgane; Jintasataporn, Orapint Animals, 9 (5), pp. 258, 2019, ISSN: 2076-2615. @article{motte_replacing_2019, title = {Replacing Fish Meal with Defatted Insect Meal (Yellow Mealworm Tenebrio molitor) Improves the Growth and Immunity of Pacific White Shrimp (Litopenaeus vannamei)}, author = {Constant Motte and Alfredo Rios and Thomas Lefebvre and Hong Do and Morgane Henry and Orapint Jintasataporn}, url = {https://www.mdpi.com/2076-2615/9/5/258}, doi = {10.3390/ani9050258}, issn = {2076-2615}, year = {2019}, date = {2019-05-01}, urldate = {2020-09-02}, journal = {Animals}, volume = {9}, number = {5}, pages = {258}, abstract = {Recently, ecological and economic issues have affected fish meal (FM) supply, the main source of protein for shrimp. This triggered a search for alternative dietary protein sources for shrimp production. We studied the consequences of replacing FM with a defatted insect meal, ŸnMealTM (YM), comprised of yellow mealworm (Tenebrio molitor). Growth and immune parameters of juvenile Pacific white shrimp (Litopenaeus vannanmei) were compared after an eight-week feeding trial. Shrimp were kept in aquaria with densities of 60 and 40 shrimp/m2 and fed one of five diets in which a proportion of FM was replaced by YM. All diets were isoproteic, isoenergetic, and balanced in lysine and methionine. After the feeding trial, shrimp were challenged with pathogenic bacteria (Vibrio parahaemolyticus). Growth and feed conversion parameters improved when YM was included in shrimp diets; with the highest weight gain and best food conversion ratio (FCR) achieved when 50% of FM was replaced by YM versus the control diet that contained no YM (initial weight: 1.60 g/shrimp; growth: 5.27 vs. 3.94 g/shrimp; FCR 1.20 vs. 1.59). In challenged shrimp, mortality rates were significantly less among groups that received YM, with a 76.9% lower mortality rate in the 50% FM replacement group versus the control.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Recently, ecological and economic issues have affected fish meal (FM) supply, the main source of protein for shrimp. This triggered a search for alternative dietary protein sources for shrimp production. We studied the consequences of replacing FM with a defatted insect meal, ŸnMealTM (YM), comprised of yellow mealworm (Tenebrio molitor). Growth and immune parameters of juvenile Pacific white shrimp (Litopenaeus vannanmei) were compared after an eight-week feeding trial. Shrimp were kept in aquaria with densities of 60 and 40 shrimp/m2 and fed one of five diets in which a proportion of FM was replaced by YM. All diets were isoproteic, isoenergetic, and balanced in lysine and methionine. After the feeding trial, shrimp were challenged with pathogenic bacteria (Vibrio parahaemolyticus). Growth and feed conversion parameters improved when YM was included in shrimp diets; with the highest weight gain and best food conversion ratio (FCR) achieved when 50% of FM was replaced by YM versus the control diet that contained no YM (initial weight: 1.60 g/shrimp; growth: 5.27 vs. 3.94 g/shrimp; FCR 1.20 vs. 1.59). In challenged shrimp, mortality rates were significantly less among groups that received YM, with a 76.9% lower mortality rate in the 50% FM replacement group versus the control. |
Kokou, Fotini; Henry, Morgane; Nikoloudaki, Chrisa; Kounna, Christiana; Vasilaki, Antigoni; Fountoulaki, Eleni Optimum protein-to-lipid ratio requirement of the juvenile shi drum (Umbrina cirrosa) as estimated by nutritional and histological parameters Journal Article Aquaculture Nutrition, 25 (2), pp. 444–455, 2019, (_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/anu.12870). @article{kokou_optimum_2019, title = {Optimum protein-to-lipid ratio requirement of the juvenile shi drum (Umbrina cirrosa) as estimated by nutritional and histological parameters}, author = {Fotini Kokou and Morgane Henry and Chrisa Nikoloudaki and Christiana Kounna and Antigoni Vasilaki and Eleni Fountoulaki}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/anu.12870}, doi = {10.1111/anu.12870}, year = {2019}, date = {2019-01-01}, journal = {Aquaculture Nutrition}, volume = {25}, number = {2}, pages = {444--455}, abstract = {Abstract The shi drum is an emerging Mediterranean aquaculture species that has gained increasing interest in recent years. However, the nutrient requirements of this species remain to be determined to optimize its culture. The present study evaluated the effects of dietary protein (470g/kg or 520g/kg) to lipid ratios (100g/kg, 150 or 200g/kg) on the growth performance, nutrient utilization and tissue morphology of the shi drum (7 g average initial weight). Dietary protein levels positively correlated with weight gain, showing high protein demands (textgreater470g/kg) for this species. Nonetheless, dietary lipid levels negatively affected growth rate, feed intake and efficiency, and protein utilization, suggesting that this macronutrient cannot be efficiently utilized in high concentrations (textgreater100g/kg). Histological evaluation revealed fat accumulation within the hepatocytes and the enterocytes with increasing dietary lipid levels, starting from 150g/kg lipid inclusion. Overall, our results confirm the high dietary protein requirements of the shi drum (textgreater470g/kg for fish of 7 g initial weight) and suggest that lipid levels should not exceed 100g/kg of inclusion in the diets as this species has a low tolerance to this macronutrient.}, note = {_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/anu.12870}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract The shi drum is an emerging Mediterranean aquaculture species that has gained increasing interest in recent years. However, the nutrient requirements of this species remain to be determined to optimize its culture. The present study evaluated the effects of dietary protein (470g/kg or 520g/kg) to lipid ratios (100g/kg, 150 or 200g/kg) on the growth performance, nutrient utilization and tissue morphology of the shi drum (7 g average initial weight). Dietary protein levels positively correlated with weight gain, showing high protein demands (textgreater470g/kg) for this species. Nonetheless, dietary lipid levels negatively affected growth rate, feed intake and efficiency, and protein utilization, suggesting that this macronutrient cannot be efficiently utilized in high concentrations (textgreater100g/kg). Histological evaluation revealed fat accumulation within the hepatocytes and the enterocytes with increasing dietary lipid levels, starting from 150g/kg lipid inclusion. Overall, our results confirm the high dietary protein requirements of the shi drum (textgreater470g/kg for fish of 7 g initial weight) and suggest that lipid levels should not exceed 100g/kg of inclusion in the diets as this species has a low tolerance to this macronutrient. |
2018 |
Henry, M A; Gai, F; Enes, P; Peréz-Jiménez, A; Gasco, L Fish & Shellfish Immunology, 83 , pp. 308–313, 2018, ISSN: 10504648. @article{henry_effect_2018, title = {Effect of partial dietary replacement of fishmeal by yellow mealworm (Tenebrio molitor) larvae meal on the innate immune response and intestinal antioxidant enzymes of rainbow trout (Oncorhynchus mykiss)}, author = {M A Henry and F Gai and P Enes and A Peréz-Jiménez and L Gasco}, url = {https://linkinghub.elsevier.com/retrieve/pii/S1050464818305849}, doi = {10.1016/j.fsi.2018.09.040}, issn = {10504648}, year = {2018}, date = {2018-12-01}, urldate = {2020-09-02}, journal = {Fish & Shellfish Immunology}, volume = {83}, pages = {308--313}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Henry, M A; Gasco, L; Chatzifotis, S; Piccolo, G Does dietary insect meal affect the fish immune system? The case of mealworm, Tenebrio molitor on European sea bass, Dicentrarchus labrax Journal Article Developmental and Comparative Immunology, 81 , pp. 204–209, 2018, ISSN: 0145305X, (Publisher: Elsevier Ltd). @article{henry_does_2018, title = {Does dietary insect meal affect the fish immune system? The case of mealworm, Tenebrio molitor on European sea bass, Dicentrarchus labrax}, author = {M A Henry and L Gasco and S Chatzifotis and G Piccolo}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85037664445&doi=10.1016%2fj.dci.2017.12.002&partnerID=40&md5=862b8ee40442d665b3ad205cf90f8095}, doi = {10.1016/j.dci.2017.12.002}, issn = {0145305X}, year = {2018}, date = {2018-01-01}, journal = {Developmental and Comparative Immunology}, volume = {81}, pages = {204--209}, abstract = {Feeding small European sea bass, Dicentrarchus labrax, for 6 weeks with Tenebrio molitor larval meal showed significant anti-inflammatory responses (ceruloplasmin, myeloperoxidase and nitric oxide). Serum bacteriolytic activity against a Gram negative bacterium was not significantly affected by dietary Tenebrio, while both lysozyme antibacterial activity and serum trypsin inhibition usually linked to the anti-parasite activity of the fish, were significantly enhanced. The latter may be due to the similarities in the composition of the exoskeleton of parasites and insects that may therefore act as an immunostimulant potentially increasing the anti-parasitic activity. The addition of exogenous proteases significantly decreased both trypsin-inhibition and serum bacteriolytic activity probably through direct inhibition of the proteins responsible for these immune functions. Further investigation involving bacterial or parasitic challenges will be necessary to assess if the effects of dietary mealworm meal on the immune system observed in the present study are translated into an improved resistance to diseases. © 2017 Elsevier Ltd}, note = {Publisher: Elsevier Ltd}, keywords = {}, pubstate = {published}, tppubtype = {article} } Feeding small European sea bass, Dicentrarchus labrax, for 6 weeks with Tenebrio molitor larval meal showed significant anti-inflammatory responses (ceruloplasmin, myeloperoxidase and nitric oxide). Serum bacteriolytic activity against a Gram negative bacterium was not significantly affected by dietary Tenebrio, while both lysozyme antibacterial activity and serum trypsin inhibition usually linked to the anti-parasite activity of the fish, were significantly enhanced. The latter may be due to the similarities in the composition of the exoskeleton of parasites and insects that may therefore act as an immunostimulant potentially increasing the anti-parasitic activity. The addition of exogenous proteases significantly decreased both trypsin-inhibition and serum bacteriolytic activity probably through direct inhibition of the proteins responsible for these immune functions. Further investigation involving bacterial or parasitic challenges will be necessary to assess if the effects of dietary mealworm meal on the immune system observed in the present study are translated into an improved resistance to diseases. © 2017 Elsevier Ltd |
2016 |
Gasco, L; Henry, M; Piccolo, G; Marono, S; Gai, F; Renna, M; Lussiana, C; Antonopoulou, E; Mola, P; Chatzifotis, S Animal Feed Science and Technology, 220 , pp. 34–45, 2016, ISSN: 03778401, (Publisher: Elsevier B.V.). @article{gasco_tenebrio_2016, title = {Tenebrio molitor meal in diets for European sea bass (Dicentrarchus labrax L.) juveniles: Growth performance, whole body composition and in vivo apparent digestibility}, author = {L Gasco and M Henry and G Piccolo and S Marono and F Gai and M Renna and C Lussiana and E Antonopoulou and P Mola and S Chatzifotis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84995467750&doi=10.1016%2fj.anifeedsci.2016.07.003&partnerID=40&md5=31b52a5867163311a054ffd4f7e540db}, doi = {10.1016/j.anifeedsci.2016.07.003}, issn = {03778401}, year = {2016}, date = {2016-01-01}, journal = {Animal Feed Science and Technology}, volume = {220}, pages = {34--45}, abstract = {Two trials were carried out on European sea bass (Dicentrarchus labrax L.) juveniles to evaluate the effects of dietary inclusion of a full-fat Tenebrio molitor (TM) larvae meal. A first growth trail was performed on 450 European sea bass using three isonitrogenous and isolipidic experimental diets (3 tanks/diet, 50 fish/tank) formulated to contain increasing levels of TM meal inclusion and precisely: 0 (TM0), 25 (TM25) and 50% (TM50) as fed basis. The performances, proximate body composition and fatty acid (FA) profile of whole fish fed the experimental diets were evaluated. A digestibility trial was then conducted on 180 fish to evaluate the in vivo apparent digestibility coefficient (ADC) of diets having 25% of TM inclusion in absence (TMD) or presence of exogenous enzymes (Carbohydrases, TM-Carb; Proteases, TM-Prot) compared to a fish meal based control diet (CD). The growth trial results showed that the highest inclusion level (TM50) led to a worsening of final body weight, weight gain, specific growth rate, and feeding rate if compared to the control diet (TM0). Regarding the whole body composition, crude protein and ether extract were not significantly influenced by the use of TM, while changes were observed in the FA profile. In particular, C18:2 n6 increased (+91% and +173% in TM25 and TM50, respectively vs TM0) with the inclusion of TM while sharp decreases of C20:5 n3 (−30% and −58% in TM25 and TM50, respectively vs TM0) and C22:6 n3 (−35% and −67% respectively vs TM0) were highlighted. Consequently, the ∑ n3/∑ n6 FA ratio showed a significant decrease (−63% and −84% in TM25 and TM50, respectively vs TM0). As far as digestibility trial is concerned, the crude protein ADC of the fish fed TMD was significantly higher than that of the fish fed CD (92.31 vs 89.97, respectively). The supplementation of digestive enzymes did not improve the protein and ADF digestibility. © 2016 Elsevier B.V.}, note = {Publisher: Elsevier B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Two trials were carried out on European sea bass (Dicentrarchus labrax L.) juveniles to evaluate the effects of dietary inclusion of a full-fat Tenebrio molitor (TM) larvae meal. A first growth trail was performed on 450 European sea bass using three isonitrogenous and isolipidic experimental diets (3 tanks/diet, 50 fish/tank) formulated to contain increasing levels of TM meal inclusion and precisely: 0 (TM0), 25 (TM25) and 50% (TM50) as fed basis. The performances, proximate body composition and fatty acid (FA) profile of whole fish fed the experimental diets were evaluated. A digestibility trial was then conducted on 180 fish to evaluate the in vivo apparent digestibility coefficient (ADC) of diets having 25% of TM inclusion in absence (TMD) or presence of exogenous enzymes (Carbohydrases, TM-Carb; Proteases, TM-Prot) compared to a fish meal based control diet (CD). The growth trial results showed that the highest inclusion level (TM50) led to a worsening of final body weight, weight gain, specific growth rate, and feeding rate if compared to the control diet (TM0). Regarding the whole body composition, crude protein and ether extract were not significantly influenced by the use of TM, while changes were observed in the FA profile. In particular, C18:2 n6 increased (+91% and +173% in TM25 and TM50, respectively vs TM0) with the inclusion of TM while sharp decreases of C20:5 n3 (−30% and −58% in TM25 and TM50, respectively vs TM0) and C22:6 n3 (−35% and −67% respectively vs TM0) were highlighted. Consequently, the ∑ n3/∑ n6 FA ratio showed a significant decrease (−63% and −84% in TM25 and TM50, respectively vs TM0). As far as digestibility trial is concerned, the crude protein ADC of the fish fed TMD was significantly higher than that of the fish fed CD (92.31 vs 89.97, respectively). The supplementation of digestive enzymes did not improve the protein and ADF digestibility. © 2016 Elsevier B.V. |
2015 |
Kokou, F; Sarropoulou, E; Cotou, E; Rigos, G; Henry, M; Alexis, M; Kentouri, M Journal of the World Aquaculture Society, 46 (2), pp. 115–128, 2015, ISSN: 08938849, (Publisher: Blackwell Publishing Inc.). @article{kokou_effects_2015, title = {Effects of fish meal replacement by a soybean protein on growth, histology, selected immune and oxidative status markers of gilthead sea bream, Sparus aurata}, author = {F Kokou and E Sarropoulou and E Cotou and G Rigos and M Henry and M Alexis and M Kentouri}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84925962477&doi=10.1111%2fjwas.12181&partnerID=40&md5=8968f20ba7b477a763db252568770b94}, doi = {10.1111/jwas.12181}, issn = {08938849}, year = {2015}, date = {2015-01-01}, journal = {Journal of the World Aquaculture Society}, volume = {46}, number = {2}, pages = {115--128}, abstract = {The purpose of the trial was to study the impact of a 6-mo dietary administration of soybean protein on growth, liver and intestine morphology, immune response, and oxidative stress in gilthead sea bream. The immune response was evaluated by performing immunological assays in blood, head kidney (HK), or serum (respiratory burst activity [RBA], myeloperoxidase content and bacteriolytic activity) and gene expression analysis of immune-associated genes (MHCIIα [major histocompatibility complex IIα], β2m [β-2-microglobulin], CSF-1R [colony-stimulating factor-1 receptor], NCCRP-1 [nonspecific cytotoxic cell receptor protein 1], TGF-β1 [transforming growth factor beta-1], and HSP70 [heat-shock protein 70]) in HK and intestine. Oxidative stress was evaluated by measuring the activity of liver enzymes associated with antioxidant system. The soybean protein was administrated in the diets at 20, 40, and 60% levels and its effects were evaluated compared with a fish meal diet. Growth and feed efficiency were affected negatively from 40% level. Increased RBA and expression levels of TGF-β1 and β2m were found in HK only at 40% level. In intestine, at 60% level CSF-1R expression was upregulated and some signs of inflammation were evident. In liver, at 60% level lipid accumulation in hepatocytes was observed and enzyme activity was increased. Dietary administration of soybean protein indicated changes from 40% in growth and immune response, and exerted an antioxidative effect at 60% level. © by the World Aquaculture Society 2015.}, note = {Publisher: Blackwell Publishing Inc.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The purpose of the trial was to study the impact of a 6-mo dietary administration of soybean protein on growth, liver and intestine morphology, immune response, and oxidative stress in gilthead sea bream. The immune response was evaluated by performing immunological assays in blood, head kidney (HK), or serum (respiratory burst activity [RBA], myeloperoxidase content and bacteriolytic activity) and gene expression analysis of immune-associated genes (MHCIIα [major histocompatibility complex IIα], β2m [β-2-microglobulin], CSF-1R [colony-stimulating factor-1 receptor], NCCRP-1 [nonspecific cytotoxic cell receptor protein 1], TGF-β1 [transforming growth factor beta-1], and HSP70 [heat-shock protein 70]) in HK and intestine. Oxidative stress was evaluated by measuring the activity of liver enzymes associated with antioxidant system. The soybean protein was administrated in the diets at 20, 40, and 60% levels and its effects were evaluated compared with a fish meal diet. Growth and feed efficiency were affected negatively from 40% level. Increased RBA and expression levels of TGF-β1 and β2m were found in HK only at 40% level. In intestine, at 60% level CSF-1R expression was upregulated and some signs of inflammation were evident. In liver, at 60% level lipid accumulation in hepatocytes was observed and enzyme activity was increased. Dietary administration of soybean protein indicated changes from 40% in growth and immune response, and exerted an antioxidative effect at 60% level. © by the World Aquaculture Society 2015. |
Rigos, G; Henry, M; Tsigenopoulos, C Sparicotyle chrysophrii and gilthead sea bream - Potential experimental infection model Journal Article Bulletin of the European Association of Fish Pathologists, 35 (2), pp. 50–54, 2015, ISSN: 01080288, (Publisher: FRS Marine Laboratory). @article{rigos_sparicotyle_2015, title = {Sparicotyle chrysophrii and gilthead sea bream - Potential experimental infection model}, author = {G Rigos and M Henry and C Tsigenopoulos}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84930162854&partnerID=40&md5=0a11a3004542dd5972bac0241c2a571e}, issn = {01080288}, year = {2015}, date = {2015-01-01}, journal = {Bulletin of the European Association of Fish Pathologists}, volume = {35}, number = {2}, pages = {50--54}, abstract = {An infection model of gilthead sea bream challenged with the monogenean Sparicotyle chrysophrii is described herein. Its design aimed to simulate the pathogenesis in situ. One thousand farmed fish (16 g) in a cement tank were heavily infected by adult parasites two weeks following the transfer of the 250 donor fish (50 g). Anorexia, lethargy and anaemia along with 15 % cumulatively mortalities were observed. A parasitological analysis carried out at end of the experiment (12 weeks) revealed that 7 % of the examined population was pathogen free and the mean intensity was 6.4 parasites/ fish. Surprisingly, growth reduction of infected fish was not correlated with the parasitic load, perhaps due to the unfavourable feeding ground for the parasite in anaemic small fish. Overall, the described infection pattern resembles the characteristics of the disease seen in the field and could be ideally adopted in controlled environments.}, note = {Publisher: FRS Marine Laboratory}, keywords = {}, pubstate = {published}, tppubtype = {article} } An infection model of gilthead sea bream challenged with the monogenean Sparicotyle chrysophrii is described herein. Its design aimed to simulate the pathogenesis in situ. One thousand farmed fish (16 g) in a cement tank were heavily infected by adult parasites two weeks following the transfer of the 250 donor fish (50 g). Anorexia, lethargy and anaemia along with 15 % cumulatively mortalities were observed. A parasitological analysis carried out at end of the experiment (12 weeks) revealed that 7 % of the examined population was pathogen free and the mean intensity was 6.4 parasites/ fish. Surprisingly, growth reduction of infected fish was not correlated with the parasitic load, perhaps due to the unfavourable feeding ground for the parasite in anaemic small fish. Overall, the described infection pattern resembles the characteristics of the disease seen in the field and could be ideally adopted in controlled environments. |
Henry, M A; Nikoloudaki, C; Tsigenopoulos, C; Rigos, G Strong effect of long-term Sparicotyle chrysophrii infection on the cellular and innate immune responses of gilthead sea bream, Sparus aurata Journal Article Developmental and Comparative Immunology, 51 (1), pp. 185–193, 2015, ISSN: 0145305X, (Publisher: Elsevier Ltd). @article{henry_strong_2015, title = {Strong effect of long-term Sparicotyle chrysophrii infection on the cellular and innate immune responses of gilthead sea bream, Sparus aurata}, author = {M A Henry and C Nikoloudaki and C Tsigenopoulos and G Rigos}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84926678862&doi=10.1016%2fj.dci.2015.03.010&partnerID=40&md5=174b70c31d6b753405608c4b64bd6416}, doi = {10.1016/j.dci.2015.03.010}, issn = {0145305X}, year = {2015}, date = {2015-01-01}, journal = {Developmental and Comparative Immunology}, volume = {51}, number = {1}, pages = {185--193}, abstract = {One thousand healthy recipient gilthead sea bream, Sparus aurata, cohabited with 250 donor fish parasitized by Sparicotyle chrysophrii (Van Beneden and Hesse, 1963) (Monogenea: Polyopisthocotylea), a common parasite of the gills of this fish species. Controls consisted of 1000 healthy fish kept in a separate tank. After 10 weeks, fish were weighed and parasite load, hemoglobin concentration and immunological parameters were assessed. Rather than the absence of parasite, hemoglobin concentration was a better marker of the health status of the fish, because S. . chrysophrii had detached from the strongly anemic gills of some animals leaving fish with affected immune system but without parasites. The parasite infection seemed to trigger a cellular response of the fish immune system but to inhibit its humoral components. Thus, parasitized fish may control the parasite infection through the action of reactive oxygen species but they may become more sensitive to potential secondary bacterial or parasitical infections. This phenomenon was demonstrated not only through significant differences between recipient and control fish but also through strong correlations between those parameters and parasite load, fish weight and/or hemoglobin concentration. © 2015 Elsevier Ltd.}, note = {Publisher: Elsevier Ltd}, keywords = {}, pubstate = {published}, tppubtype = {article} } One thousand healthy recipient gilthead sea bream, Sparus aurata, cohabited with 250 donor fish parasitized by Sparicotyle chrysophrii (Van Beneden and Hesse, 1963) (Monogenea: Polyopisthocotylea), a common parasite of the gills of this fish species. Controls consisted of 1000 healthy fish kept in a separate tank. After 10 weeks, fish were weighed and parasite load, hemoglobin concentration and immunological parameters were assessed. Rather than the absence of parasite, hemoglobin concentration was a better marker of the health status of the fish, because S. . chrysophrii had detached from the strongly anemic gills of some animals leaving fish with affected immune system but without parasites. The parasite infection seemed to trigger a cellular response of the fish immune system but to inhibit its humoral components. Thus, parasitized fish may control the parasite infection through the action of reactive oxygen species but they may become more sensitive to potential secondary bacterial or parasitical infections. This phenomenon was demonstrated not only through significant differences between recipient and control fish but also through strong correlations between those parameters and parasite load, fish weight and/or hemoglobin concentration. © 2015 Elsevier Ltd. |
Henry, M; Gasco, L; Piccolo, G; Fountoulaki, E Review on the use of insects in the diet of farmed fish: Past and future Journal Article Animal Feed Science and Technology, 203 (1), pp. 1–22, 2015, ISSN: 03778401, (Publisher: Elsevier B.V.). @article{henry_review_2015, title = {Review on the use of insects in the diet of farmed fish: Past and future}, author = {M Henry and L Gasco and G Piccolo and E Fountoulaki}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84928339785&doi=10.1016%2fj.anifeedsci.2015.03.001&partnerID=40&md5=ca52f92d79e1c44dd55c764f9c79ff2b}, doi = {10.1016/j.anifeedsci.2015.03.001}, issn = {03778401}, year = {2015}, date = {2015-01-01}, journal = {Animal Feed Science and Technology}, volume = {203}, number = {1}, pages = {1--22}, abstract = {The decrease in the availability and the increase in the prices of fishmeal and fish oil have prompted the search for sustainable alternatives for aquaculture feeds. Insects, which are part of the natural diet of fish, leave a small ecological footprint and have a limited need for arable land, may represent a good candidate. Over the last decade, studies of the replacement of fishmeal with insects in the diet of fish have emerged and the promising results have encouraged further research. The present review displays these results in tables and emphasizes the achievable dietary inclusion levels. It discusses the potential of locusts, grasshoppers, termites, yellow mealworms, Asiatic rhinoceros beetles, superworms, domesticated silkworms, common houseflies, common mosquitoes and black soldier flies for use as fishmeal and/or fish oil replacement in the fish diet. The review succinctly compares the composition of the insects with the requirements of the fish (proteins and amino acids, lipids and fatty acids, vitamins and minerals). This review also discusses the potential hurdles of using insects in fish feeds (toxicity of insects through bioaccumulation, deficiencies in amino acids or fatty acids, chitin content, palatability, digestibility), a nd the available ways of avoiding these drawbacks (control of the dietary substrate of insects in mass rearing units, manipulation of the diet of insects, mixture of dietary proteins, use of aquatic insects, processing of insect meal). Finally, it suggests paths worthy of future research on these new fishmeal alternatives. © 2015 Elsevier B.V.}, note = {Publisher: Elsevier B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The decrease in the availability and the increase in the prices of fishmeal and fish oil have prompted the search for sustainable alternatives for aquaculture feeds. Insects, which are part of the natural diet of fish, leave a small ecological footprint and have a limited need for arable land, may represent a good candidate. Over the last decade, studies of the replacement of fishmeal with insects in the diet of fish have emerged and the promising results have encouraged further research. The present review displays these results in tables and emphasizes the achievable dietary inclusion levels. It discusses the potential of locusts, grasshoppers, termites, yellow mealworms, Asiatic rhinoceros beetles, superworms, domesticated silkworms, common houseflies, common mosquitoes and black soldier flies for use as fishmeal and/or fish oil replacement in the fish diet. The review succinctly compares the composition of the insects with the requirements of the fish (proteins and amino acids, lipids and fatty acids, vitamins and minerals). This review also discusses the potential hurdles of using insects in fish feeds (toxicity of insects through bioaccumulation, deficiencies in amino acids or fatty acids, chitin content, palatability, digestibility), a nd the available ways of avoiding these drawbacks (control of the dietary substrate of insects in mass rearing units, manipulation of the diet of insects, mixture of dietary proteins, use of aquatic insects, processing of insect meal). Finally, it suggests paths worthy of future research on these new fishmeal alternatives. © 2015 Elsevier B.V. |
Eslamloo, Khalil; Akhavan, Sobhan R; Eslamifar, Ahmad; Henry, Morgane A Aquaculture Research, 46 (1), pp. 202–215, 2015, ISSN: 1355557X. @article{eslamloo_effects_2015, title = {Effects of background colour on growth performance, skin pigmentation, physiological condition and innate immune responses of goldfish, Carassius auratus}, author = {Khalil Eslamloo and Sobhan R Akhavan and Ahmad Eslamifar and Morgane A Henry}, url = {http://doi.wiley.com/10.1111/are.12177}, doi = {10.1111/are.12177}, issn = {1355557X}, year = {2015}, date = {2015-01-01}, urldate = {2020-08-18}, journal = {Aquaculture Research}, volume = {46}, number = {1}, pages = {202--215}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2014 |
Henry, Morgane; Fountoulaki, Eleni Fish & Shellfish Immunology, 37 (2), pp. 215–219, 2014, ISSN: 10504648. @article{henry_optimal_2014, title = {Optimal dietary protein/lipid ratio for improved immune status of a newly cultivated Mediterranean fish species, the shi drum Umbrina cirrosa, L.}, author = {Morgane Henry and Eleni Fountoulaki}, url = {https://linkinghub.elsevier.com/retrieve/pii/S1050464814000370}, doi = {10.1016/j.fsi.2014.02.005}, issn = {10504648}, year = {2014}, date = {2014-04-01}, urldate = {2020-08-18}, journal = {Fish & Shellfish Immunology}, volume = {37}, number = {2}, pages = {215--219}, abstract = {Shi drum is a new fish species recently cultivated in Greek fish farms. Its feeding requirements and health status must be determined in order to optimize its culture conditions. The present study looked at the effects of different dietary protein levels (47 or 52%) in combination with 3 lipid levels (10, 15 or 20%) on the immune system of juvenile shi drums. Most immune parameters assessed were affected by the dietary treatment. The ceruloplasmin activity, the whole blood respiratory burst activity, the serum myeloperoxidase activity, the antibacterial activity of serum lysozyme and the serum antiprotease activity all varied with the dietary protein/lipid ratio. In view of these results, the lowest dietary lipid level tested (10%) was optimal for the immune system of shi drum but a clear determination of the optimal dietary protein levels was not obtained as 47% reduced stress but also increased the ceruloplasmin response of the fish. A dietary lipid level of 10% lipids was recommended for optimal immune status of the fish. Dietary protein level of 47% seemed to give better immune responses but further study is needed to refine protein requirements. © 2014 Elsevier Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Shi drum is a new fish species recently cultivated in Greek fish farms. Its feeding requirements and health status must be determined in order to optimize its culture conditions. The present study looked at the effects of different dietary protein levels (47 or 52%) in combination with 3 lipid levels (10, 15 or 20%) on the immune system of juvenile shi drums. Most immune parameters assessed were affected by the dietary treatment. The ceruloplasmin activity, the whole blood respiratory burst activity, the serum myeloperoxidase activity, the antibacterial activity of serum lysozyme and the serum antiprotease activity all varied with the dietary protein/lipid ratio. In view of these results, the lowest dietary lipid level tested (10%) was optimal for the immune system of shi drum but a clear determination of the optimal dietary protein levels was not obtained as 47% reduced stress but also increased the ceruloplasmin response of the fish. A dietary lipid level of 10% lipids was recommended for optimal immune status of the fish. Dietary protein level of 47% seemed to give better immune responses but further study is needed to refine protein requirements. © 2014 Elsevier Ltd. |
Eslamloo, K; Akhavan, S R; Fallah, F J; Henry, M A Variations of physiological and innate immunological responses in goldfish (Carassius auratus) subjected to recurrent acute stress Journal Article Fish and Shellfish Immunology, 37 (1), pp. 147–153, 2014, ISSN: 10504648. @article{eslamloo_variations_2014, title = {Variations of physiological and innate immunological responses in goldfish (Carassius auratus) subjected to recurrent acute stress}, author = {K Eslamloo and S R Akhavan and F J Fallah and M A Henry}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84894069671&doi=10.1016%2fj.fsi.2014.01.014&partnerID=40&md5=83f2cf0a127d10a4753985f22fb5abaa}, doi = {10.1016/j.fsi.2014.01.014}, issn = {10504648}, year = {2014}, date = {2014-01-01}, journal = {Fish and Shellfish Immunology}, volume = {37}, number = {1}, pages = {147--153}, abstract = {This study was undertaken to investigate the influence of repeated acute stress on the physiological status and non-specific immune response of goldfish, Carassius auratus. The acute stress was a succession of a 3min-chasing period followed by a 2min-air exposure. The goldfish in triplicate tanks were subjected 3 times daily to this stress for one (S3) or three (S9) days. A separate group of unstressed fish was used as control for each sampling time. Blood samples were collected 12, 48 and 120h after the last stress procedure. Variations of globulin levels, plasma anti-protease and bactericidal activities were not significant in the present study. The haematological parameters and plasma total protein and albumin strongly declined in S9 fish 12h post-stress compared to control fish. However, plasma cortisol, glucose and lactate levels in both S3 and S9 transiently increased compared to the control fish. Similarly, plasma peroxidase activity transiently increased in both stressed groups 12h after stress. An increase in plasma lysozyme and complement activities suggested a hormesis-like effect with one-day acute stress improving the immunological response of goldfish while an extension of the stress period to three days impaired physiology and immunity for up to 5 days. This study revealed that recurrent acute stress could immunosuppress goldfish as usually expected of chronic stress. © 2014 Elsevier Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study was undertaken to investigate the influence of repeated acute stress on the physiological status and non-specific immune response of goldfish, Carassius auratus. The acute stress was a succession of a 3min-chasing period followed by a 2min-air exposure. The goldfish in triplicate tanks were subjected 3 times daily to this stress for one (S3) or three (S9) days. A separate group of unstressed fish was used as control for each sampling time. Blood samples were collected 12, 48 and 120h after the last stress procedure. Variations of globulin levels, plasma anti-protease and bactericidal activities were not significant in the present study. The haematological parameters and plasma total protein and albumin strongly declined in S9 fish 12h post-stress compared to control fish. However, plasma cortisol, glucose and lactate levels in both S3 and S9 transiently increased compared to the control fish. Similarly, plasma peroxidase activity transiently increased in both stressed groups 12h after stress. An increase in plasma lysozyme and complement activities suggested a hormesis-like effect with one-day acute stress improving the immunological response of goldfish while an extension of the stress period to three days impaired physiology and immunity for up to 5 days. This study revealed that recurrent acute stress could immunosuppress goldfish as usually expected of chronic stress. © 2014 Elsevier Ltd. |
2013 |
Eslamloo, Khalil; Akhavan, Sobhan R; Henry, Morgane A Acta Ichthyologica Et Piscatoria, 43 (3), pp. 211–218, 2013, ISSN: 01371592, 17341515. @article{eslamloo_effects_2013, title = {Effects of Dietary Administration of Bacillus Probiotics on the Non-Specific Immune Responses of Tinfoil Barb, Barbonymus schwanenfeldii (Actinopterygii: Cypriniformes: Cyprinidae)}, author = {Khalil Eslamloo and Sobhan R Akhavan and Morgane A Henry}, url = {http://www.aiep.pl/volumes/2010/4_3/txt/txt_05.php}, doi = {10.3750/AIP2013.43.3.05}, issn = {01371592, 17341515}, year = {2013}, date = {2013-09-01}, urldate = {2020-08-18}, journal = {Acta Ichthyologica Et Piscatoria}, volume = {43}, number = {3}, pages = {211--218}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Cotou, E; Tsangaris, C; Henry, M Comparative study of biochemical and immunological biomarkers in three marine bivalves exposed at a polluted site Journal Article Environmental Science and Pollution Research, 20 (3), pp. 1812–1822, 2013, ISSN: 09441344. @article{cotou_comparative_2013, title = {Comparative study of biochemical and immunological biomarkers in three marine bivalves exposed at a polluted site}, author = {E Cotou and C Tsangaris and M Henry}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84874322576&doi=10.1007%2fs11356-012-1150-3&partnerID=40&md5=df1b5b97f643a90c262b2b7c27443e42}, doi = {10.1007/s11356-012-1150-3}, issn = {09441344}, year = {2013}, date = {2013-01-01}, journal = {Environmental Science and Pollution Research}, volume = {20}, number = {3}, pages = {1812--1822}, abstract = {A battery of biochemical and immunological biomarkers used for pollution assessment were measured for first time in the clams Venus verrucosa and Callista chione and were compared with those of the mussel Mytilus galloprovincialis, a well-established indicator organism utilized in numerous environmental monitoring programs. Clams and mussel were transplanted at a polluted and a reference site or maintained at the laboratory. Among biochemical biomarkers, acetylcholinesterase did not differ at the polluted site in all species, but there was a significant difference between the mussel and the clams, glutathione S-transferase showed a clear inhibition at the polluted site in all species and a significant difference between the two clams was also indicated, while catalase activities were increased only in V. verrucosa at the polluted site and not in mussel or the other clam. Immunological biomarkers responses were also pronounced at the polluted site. Lysozyme activity was species-dependent whereas respiratory burst activity measured as luminol-dependent chemiluminescence (CL) was site and stimulus dependent, and it was evident in M. galloprovincialis and V. verrucosa and not in C. chione. Further investigation focused on biochemical and immunological biomarkers related with the oxidative mechanisms in clams will strengthen and expand their use as bioindicators for pollution assessment. © 2012 Springer-Verlag.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A battery of biochemical and immunological biomarkers used for pollution assessment were measured for first time in the clams Venus verrucosa and Callista chione and were compared with those of the mussel Mytilus galloprovincialis, a well-established indicator organism utilized in numerous environmental monitoring programs. Clams and mussel were transplanted at a polluted and a reference site or maintained at the laboratory. Among biochemical biomarkers, acetylcholinesterase did not differ at the polluted site in all species, but there was a significant difference between the mussel and the clams, glutathione S-transferase showed a clear inhibition at the polluted site in all species and a significant difference between the two clams was also indicated, while catalase activities were increased only in V. verrucosa at the polluted site and not in mussel or the other clam. Immunological biomarkers responses were also pronounced at the polluted site. Lysozyme activity was species-dependent whereas respiratory burst activity measured as luminol-dependent chemiluminescence (CL) was site and stimulus dependent, and it was evident in M. galloprovincialis and V. verrucosa and not in C. chione. Further investigation focused on biochemical and immunological biomarkers related with the oxidative mechanisms in clams will strengthen and expand their use as bioindicators for pollution assessment. © 2012 Springer-Verlag. |
Rigos, G; Zonaras, V; Nikoloudaki, X; Cotou, E; Henry, M; Varo, I; Alexis, M Distribution and depletion of sulfadiazine after a multiple per os dosing in gilthead sea bream (Sparus aurata) fed two different diets Journal Article Mediterranean Marine Science, 14 (2), pp. 377–383, 2013, ISSN: 1108393X. @article{rigos_distribution_2013, title = {Distribution and depletion of sulfadiazine after a multiple per os dosing in gilthead sea bream (Sparus aurata) fed two different diets}, author = {G Rigos and V Zonaras and X Nikoloudaki and E Cotou and M Henry and I Varo and M Alexis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84880738781&doi=10.12681%2fmms.490&partnerID=40&md5=4c0455add70b6cdc2410df863d88cc13}, doi = {10.12681/mms.490}, issn = {1108393X}, year = {2013}, date = {2013-01-01}, journal = {Mediterranean Marine Science}, volume = {14}, number = {2}, pages = {377--383}, abstract = {The distribution and depletion profile of sulfadiazine (SDZ) were investigated in gilthead sea bream (Sparus aurata) fed on fish oil (FO) or plant oil-based (PO) diets. Fish averaging 230 g were given medicated feed containing 25 mg SDZ kg-1 fish for 5 days at 24-26°C. Blood and muscle plus skin were sampled on days 1, 3, 5, 6, 8 and 9. Differences in plasma and fillet SDZ levels between the two groups were statistically insignificant. The maximum drug concentrations in plasma were 3.2 ± 1.9 μg mL-1 and 2.9 ± 1.2 μg mL-1 in the PO and FO groups, respectively. In post-medicated samples depletion rapidly reached concentrations close to the level of quantification at 72 h post medication. Withdrawal times to reach consumer safety levels were calculated to be 103 and 118 h for the FO and the PO groups, respectively. N4-acetylation was found to be the major metabolic pathway of SDZ in gilthead sea bream fillet accounting for 23 and 19% of the parent compound in the FO and the PO groups, respectively. Overall, alteration of the dietary lipid profile induced insignificant effects on the kinetics of SDZ. The high tissue SDZ levels during medication and the fast removal of the parent compound and its metabolites from edible tissues of gilthead sea bream reflect a promising antibacterial profile.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The distribution and depletion profile of sulfadiazine (SDZ) were investigated in gilthead sea bream (Sparus aurata) fed on fish oil (FO) or plant oil-based (PO) diets. Fish averaging 230 g were given medicated feed containing 25 mg SDZ kg-1 fish for 5 days at 24-26°C. Blood and muscle plus skin were sampled on days 1, 3, 5, 6, 8 and 9. Differences in plasma and fillet SDZ levels between the two groups were statistically insignificant. The maximum drug concentrations in plasma were 3.2 ± 1.9 μg mL-1 and 2.9 ± 1.2 μg mL-1 in the PO and FO groups, respectively. In post-medicated samples depletion rapidly reached concentrations close to the level of quantification at 72 h post medication. Withdrawal times to reach consumer safety levels were calculated to be 103 and 118 h for the FO and the PO groups, respectively. N4-acetylation was found to be the major metabolic pathway of SDZ in gilthead sea bream fillet accounting for 23 and 19% of the parent compound in the FO and the PO groups, respectively. Overall, alteration of the dietary lipid profile induced insignificant effects on the kinetics of SDZ. The high tissue SDZ levels during medication and the fast removal of the parent compound and its metabolites from edible tissues of gilthead sea bream reflect a promising antibacterial profile. |
2012 |
Henry, M A; Nikolopoulou, D; Alexis, M N In vitro effect of peas, Pisum pisum, and chickpeas, Cicer arietinum, on the immune system of gilthead seabream, Sparus aurata Journal Article In Vitro Cellular & Developmental Biology - Animal, 48 (7), pp. 407–412, 2012, ISSN: 1071-2690, 1543-706X. @article{henry_vitro_2012, title = {In vitro effect of peas, Pisum pisum, and chickpeas, Cicer arietinum, on the immune system of gilthead seabream, Sparus aurata}, author = {M A Henry and D Nikolopoulou and M N Alexis}, url = {http://link.springer.com/10.1007/s11626-012-9528-6}, doi = {10.1007/s11626-012-9528-6}, issn = {1071-2690, 1543-706X}, year = {2012}, date = {2012-08-01}, urldate = {2020-08-18}, journal = {In Vitro Cellular & Developmental Biology - Animal}, volume = {48}, number = {7}, pages = {407--412}, abstract = {The future for a sustainable aquaculture relies on the formulation of feed including alternatives to fish meal and fish oil that do not impair fish growth and that improve fish health status. Grain legumes such as field peas and chickpeas offer good sources of proteins, carbohydrates, fibers, vitamins, and minerals. The effect of peas and chickpeas on the immune system of seabream was assessed in vitro in order to detect any potential immunosuppressing problem. Peas was determined to be a better fishmeal alternative than chickpeas as they induced higher respiratory burst measured by the nitro blue tetrazolium assay and primed the Phorbol 12-myristate 13- acetate (PMA)-stimulated intracellular respiratory burst whereas chickpeas neither directly stimulated respiratory burst nor primed it. However, when the intra- and extracellular respiratory burst activities were taken into account, high concentrations of peas inhibited the zymosan- and PMA-triggered chemiluminescence. This apparent reduction of the production of reactive oxygen species may reflect in fact the antioxidant activity of legumes. This, together with the absence of effect on the phagocytosis activity, suggested that peas are not immunosuppressing gilthead seabream. Further in vivo studies preferably comporting a bacterial challenge will have to ascertain the absence of immunosuppressing effect of these legumes. © The Society for In Vitro Biology 2012.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The future for a sustainable aquaculture relies on the formulation of feed including alternatives to fish meal and fish oil that do not impair fish growth and that improve fish health status. Grain legumes such as field peas and chickpeas offer good sources of proteins, carbohydrates, fibers, vitamins, and minerals. The effect of peas and chickpeas on the immune system of seabream was assessed in vitro in order to detect any potential immunosuppressing problem. Peas was determined to be a better fishmeal alternative than chickpeas as they induced higher respiratory burst measured by the nitro blue tetrazolium assay and primed the Phorbol 12-myristate 13- acetate (PMA)-stimulated intracellular respiratory burst whereas chickpeas neither directly stimulated respiratory burst nor primed it. However, when the intra- and extracellular respiratory burst activities were taken into account, high concentrations of peas inhibited the zymosan- and PMA-triggered chemiluminescence. This apparent reduction of the production of reactive oxygen species may reflect in fact the antioxidant activity of legumes. This, together with the absence of effect on the phagocytosis activity, suggested that peas are not immunosuppressing gilthead seabream. Further in vivo studies preferably comporting a bacterial challenge will have to ascertain the absence of immunosuppressing effect of these legumes. © The Society for In Vitro Biology 2012. |
Vectesi, D; Kotzamanis, I; Henry, M A; Vatsos, I N Haematological and immunological responses of sea bass (Dicentrarchus labrax) to a short-term exposure to increased water levels of nitrate Journal Article Iranian Journal of Fisheries Sciences, 11 (2), pp. 430–439, 2012, ISSN: 15622916. @article{vectesi_haematological_2012, title = {Haematological and immunological responses of sea bass (Dicentrarchus labrax) to a short-term exposure to increased water levels of nitrate}, author = {D Vectesi and I Kotzamanis and M A Henry and I N Vatsos}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84867551222&partnerID=40&md5=7290fe927b272531401b44e7a7be750d}, issn = {15622916}, year = {2012}, date = {2012-01-01}, journal = {Iranian Journal of Fisheries Sciences}, volume = {11}, number = {2}, pages = {430--439}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Cotou, E; Henry, M; Zeri, C; Rigos, G; Torreblanca, A; Catsiki, V -A Chemosphere, 89 , pp. 1091 – 1097, 2012. @article{cotou_short-term_2012, title = {Short-term exposure of the European sea bass Dicentrarchus labrax to copper-based antifouling treated nets: Copper bioavailability and biomarkers responses}, author = {E Cotou and M Henry and C Zeri and G Rigos and A Torreblanca and V -A Catsiki}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84865737122&partnerID=40&md5=756a3c35cf896d33539cf359b2ee5701}, doi = {10.1016/j.chemosphere.2012.05.075}, year = {2012}, date = {2012-01-01}, journal = {Chemosphere}, volume = {89}, pages = {1091 -- 1097}, abstract = {We studied if the levels of copper released from antifouling treated nets used in finfish mariculture could affect the immune defense mechanism and/or induce oxidative stress in Dicentrarchus labrax, after short term exposure in laboratory experiments. Dissolved copper concentration released from the treated nets, copper bioavailability and a set of biomarkers responses were measured. Biomarkers included hemoglobin concentration, activities of lysozyme, total complement, respiratory burst, glutathione S-transferase and acetycholinesterase and concentration of thiobarbituric acid reactive substances. Results indicated elevated copper concentration in seawater (184μgL-1) but low concentration in muscle (1.5μgg-1) and liver (117μgg-1). Copper bioavailability was independent of copper complexes with dissolved organic carbon. However, formation of copper complexes with other matrices could neither be excluded nor justified. The released copper from the treated nets did not induce oxidative stress but affected the immediate immune defense mechanism of the exposed fish making them more easily vulnerable to diseases. Consequently, copper-based antifouling treated nets could be a risk factor for D. labrax health. © 2012 Elsevier Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We studied if the levels of copper released from antifouling treated nets used in finfish mariculture could affect the immune defense mechanism and/or induce oxidative stress in Dicentrarchus labrax, after short term exposure in laboratory experiments. Dissolved copper concentration released from the treated nets, copper bioavailability and a set of biomarkers responses were measured. Biomarkers included hemoglobin concentration, activities of lysozyme, total complement, respiratory burst, glutathione S-transferase and acetycholinesterase and concentration of thiobarbituric acid reactive substances. Results indicated elevated copper concentration in seawater (184μgL-1) but low concentration in muscle (1.5μgg-1) and liver (117μgg-1). Copper bioavailability was independent of copper complexes with dissolved organic carbon. However, formation of copper complexes with other matrices could neither be excluded nor justified. The released copper from the treated nets did not induce oxidative stress but affected the immediate immune defense mechanism of the exposed fish making them more easily vulnerable to diseases. Consequently, copper-based antifouling treated nets could be a risk factor for D. labrax health. © 2012 Elsevier Ltd. |
Kokou, F; Rigos, G; Henry, M; Kentouri, M; Alexis, M Aquaculture, 364-365 , pp. 74 – 81, 2012. @article{kokou_growth_2012, title = {Growth performance, feed utilization and non-specific immune response of gilthead sea bream (Sparus aurata L.) fed graded levels of a bioprocessed soybean meal}, author = {F Kokou and G Rigos and M Henry and M Kentouri and M Alexis}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84865338480&partnerID=40&md5=3766be14a1b238e69520da3727139bf6}, doi = {10.1016/j.aquaculture.2012.08.009}, year = {2012}, date = {2012-01-01}, journal = {Aquaculture}, volume = {364-365}, pages = {74 -- 81}, abstract = {The objective of this study was to evaluate the effects of partial fish meal replacement by a bioprocessed soy product (BS) in juvenile gilthead sea bream over the course of a 3-month trial. A diet with fish meal as the sole protein source (FM) was compared to diets with 20% (BS20), 40% (BS40) and 60% (BS60) of replacement. The effects of diets on growth performance, feed utilization, gut integrity, liver structure and immune status were evaluated. Weight gain, feed efficiency, daily growth index and specific growth rate decreased at the highest level of BS, while feed intake increased. In fish from BS40 and BS60 groups, indices indicating growth in response to protein intake such as protein efficiency ratio and protein productive value were decreased significantly. Body composition did not differ among groups for all parameters analysed. As far as non-specific immune response is concerned, spontaneous respiratory burst activity of blood significantly decreased at BS20 and BS40 groups, while stimulated activity did not indicate any significant change. Serum myeloperoxidase levels and total bacteriolytic activity of complement increased significantly at the highest dietary inclusion of BS, compared to the control group, while serum lysozyme levels did not indicate significant changes. Liver structure was not affected at any level, but some alterations in intestinal morphology were observed in the distal intestine mainly in the BS60 group. Overall, FM replacement by BS affected negatively gilthead sea bream growth performance at the 60% level, protein efficiency from the 40% level and affected some indicators of the immune response. © 2012 Elsevier B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The objective of this study was to evaluate the effects of partial fish meal replacement by a bioprocessed soy product (BS) in juvenile gilthead sea bream over the course of a 3-month trial. A diet with fish meal as the sole protein source (FM) was compared to diets with 20% (BS20), 40% (BS40) and 60% (BS60) of replacement. The effects of diets on growth performance, feed utilization, gut integrity, liver structure and immune status were evaluated. Weight gain, feed efficiency, daily growth index and specific growth rate decreased at the highest level of BS, while feed intake increased. In fish from BS40 and BS60 groups, indices indicating growth in response to protein intake such as protein efficiency ratio and protein productive value were decreased significantly. Body composition did not differ among groups for all parameters analysed. As far as non-specific immune response is concerned, spontaneous respiratory burst activity of blood significantly decreased at BS20 and BS40 groups, while stimulated activity did not indicate any significant change. Serum myeloperoxidase levels and total bacteriolytic activity of complement increased significantly at the highest dietary inclusion of BS, compared to the control group, while serum lysozyme levels did not indicate significant changes. Liver structure was not affected at any level, but some alterations in intestinal morphology were observed in the distal intestine mainly in the BS60 group. Overall, FM replacement by BS affected negatively gilthead sea bream growth performance at the 60% level, protein efficiency from the 40% level and affected some indicators of the immune response. © 2012 Elsevier B.V. |
Rigos, G; Zonaras, V; Cotou, E; Henry, M; Nikoloudaki, X; Alexis, M Dietary fish oil replacement does not alter quinolone uptake in gilthead seabream Journal Article Bulletin of the European Association of Fish Pathologists, 32 , pp. 94 – 102, 2012. @article{rigos_dietary_2012, title = {Dietary fish oil replacement does not alter quinolone uptake in gilthead seabream}, author = {G Rigos and V Zonaras and E Cotou and M Henry and X Nikoloudaki and M Alexis}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84862734496&partnerID=40&md5=d73786c730ef0aecbefe7d2fd30181f7}, year = {2012}, date = {2012-01-01}, journal = {Bulletin of the European Association of Fish Pathologists}, volume = {32}, pages = {94 -- 102}, abstract = {The effects of fish oil replacement on the availability of oxolinic acid (OA) and flumequine (FLU) were investigated in gilthead sea bream (Sparus aurata). Two experimental feeds were used containing 15% (FO diet) or 5% fish oil (VO diet), the later replaced by rapeseed, linseed and palm oil. Fish received through the diet a dosage of 30 or 75 mg OA/kg fish for 7 days at 26°C water temperature. FLU was administered at 30 mg/kg fish for 5 days at 26 or 13°C. Fish given FO diets showed insignificantly higher OA plasma levels compared to those receiving VO feeds. Maximum OA plasma concentrations were measured to be 0.13 and 0.08 ug/ml for the FO and VO groups, respectively at standard dosage. A small increase of OA levels in plasma was observed in FO and VO group (0.14 vs.0.13 μg/ml) when fish were administered double drug dosing. Circulatory FLU levels were also measured to be low and insignificantly different between the two groups at the low water temperature. They were higher compared to those measured at 26°C with peaked concentrations of 0.15 and 0.17 μg/ml for FO and VO groups, respectively vs. 0.085 and 0.083 μg/ ml at 13 °C. Overall, low quinolone levels were measured in circulation of gilthead sea bream fed alternative diets. Reduction of the dietary fish oil level induced insignificant effects on the uptake of OA and FLU in this species.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The effects of fish oil replacement on the availability of oxolinic acid (OA) and flumequine (FLU) were investigated in gilthead sea bream (Sparus aurata). Two experimental feeds were used containing 15% (FO diet) or 5% fish oil (VO diet), the later replaced by rapeseed, linseed and palm oil. Fish received through the diet a dosage of 30 or 75 mg OA/kg fish for 7 days at 26°C water temperature. FLU was administered at 30 mg/kg fish for 5 days at 26 or 13°C. Fish given FO diets showed insignificantly higher OA plasma levels compared to those receiving VO feeds. Maximum OA plasma concentrations were measured to be 0.13 and 0.08 ug/ml for the FO and VO groups, respectively at standard dosage. A small increase of OA levels in plasma was observed in FO and VO group (0.14 vs.0.13 μg/ml) when fish were administered double drug dosing. Circulatory FLU levels were also measured to be low and insignificantly different between the two groups at the low water temperature. They were higher compared to those measured at 26°C with peaked concentrations of 0.15 and 0.17 μg/ml for FO and VO groups, respectively vs. 0.085 and 0.083 μg/ ml at 13 °C. Overall, low quinolone levels were measured in circulation of gilthead sea bream fed alternative diets. Reduction of the dietary fish oil level induced insignificant effects on the uptake of OA and FLU in this species. |
2011 |
Adamidou, S; Nengas, I; Henry, M; Midoy, Ioakei N; Rigos, G; Bell, G J; Jauncey, K Effects of dietary inclusion of peas, chickpeas and faba beans on growth, feed utilization and health of gilthead seabream (Sparus aurata) Journal Article Aquaculture Nutrition, 17 , pp. e288 – e296, 2011. @article{adamidou_effects_2011, title = {Effects of dietary inclusion of peas, chickpeas and faba beans on growth, feed utilization and health of gilthead seabream (Sparus aurata)}, author = {S Adamidou and I Nengas and M Henry and N Ioakei Midoy and G Rigos and G J Bell and K Jauncey}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79952502639&partnerID=40&md5=3f40d3886ed69bb45394174909f89c68}, doi = {10.1111/j.1365-2095.2010.00762.x}, year = {2011}, date = {2011-01-01}, journal = {Aquaculture Nutrition}, volume = {17}, pages = {e288 -- e296}, abstract = {Three legumes [field peas (P), chickpeas (CP) and faba beans (B)] at two inclusion levels [170gkg -1 (L) and 350gkg -1 (H)] were evaluated in a 13-week experiment with triplicate groups of 92.6±5.0g gilthead seabream (Sparus aurata L.). A control diet included wheat meal, fishmeal (FM) and a mixture of plant ingredients as protein sources. Diets were formulated to be isonitrogenous and isoenergetic and processed in a twin-screw extruder. Restricted feeding was applied (15gkg -1 of body weight) and growth, haematology and histology parameters were evaluated. Decreased, but not significant, growth values were observed for all diets including legumes compared to the control. Poorer feed conversion ratio values were observed for both P diets and for high level B diet. Liver glycogen increased with increasing starch level, but hepatosomatic index did not differ significantly for any of the diet treatments. Histological examination of internal organs showed no pathological abnormalities that could be related to nutritional treatment. The study indicated that the tested legumes are ingredients that could be used in farmed seabream diets up to 350gkg -1 without negative effects replacing other carbohydrate sources and part of FM. © 2010 Blackwell Publishing Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Three legumes [field peas (P), chickpeas (CP) and faba beans (B)] at two inclusion levels [170gkg -1 (L) and 350gkg -1 (H)] were evaluated in a 13-week experiment with triplicate groups of 92.6±5.0g gilthead seabream (Sparus aurata L.). A control diet included wheat meal, fishmeal (FM) and a mixture of plant ingredients as protein sources. Diets were formulated to be isonitrogenous and isoenergetic and processed in a twin-screw extruder. Restricted feeding was applied (15gkg -1 of body weight) and growth, haematology and histology parameters were evaluated. Decreased, but not significant, growth values were observed for all diets including legumes compared to the control. Poorer feed conversion ratio values were observed for both P diets and for high level B diet. Liver glycogen increased with increasing starch level, but hepatosomatic index did not differ significantly for any of the diet treatments. Histological examination of internal organs showed no pathological abnormalities that could be related to nutritional treatment. The study indicated that the tested legumes are ingredients that could be used in farmed seabream diets up to 350gkg -1 without negative effects replacing other carbohydrate sources and part of FM. © 2010 Blackwell Publishing Ltd. |
Rigos, G; Zonaras, V; Nikolopoulou, D; Henry, M; Nikoloudaki, X; Alexis, M Aquaculture, 311 , pp. 31 – 35, 2011. @article{rigos_effect_2011, title = {The effect of diet composition (plant vs fish oil-based diets) on the availability of oxytetracycline in gilthead sea bream (Sparus aurata) at two water temperatures}, author = {G Rigos and V Zonaras and D Nikolopoulou and M Henry and X Nikoloudaki and M Alexis}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-78651511216&partnerID=40&md5=1ff8997553f46392794bf523d922f6ad}, doi = {10.1016/j.aquaculture.2010.11.018}, year = {2011}, date = {2011-01-01}, journal = {Aquaculture}, volume = {311}, pages = {31 -- 35}, abstract = {The effect of partial fish oil replacement (66%) by rapeseed, linseed and palm oil on the availability of oxytetracycline (OTC) was investigated in gilthead sea bream (Sparus aurata). Fish received a dosage of 75. mg OTC/kg for 5. days at 27 or 14°C water temperature. Tissues including blood and muscle plus skin were sampled on days 1, 3 and 5 during treatment and on days 6, 8, 12 and 16 post-treatment. At the high water temperature, OTC levels were undetectable in the tested tissues at all sampling points for both diets. At the winter temperature, plasma OTC concentrations were insignificantly higher in fish given plant oil-based diets compared to fish oil diets with maximum values of 1.03 and 0.73μg/ml respectively on day 6 for both groups. Similarly, OTC muscle plus skin levels were insignificantly lower in the fish oil compared to the plant oil group with respective values of 0.48μg/g on day 5 and 1.21μg/g on day 6. Overall, replacement of fish oil by plant oil sources showed no significant influence on OTC availability in gilthead sea bream at winter temperatures. The use of OTC should be discouraged at very high water temperatures in this species irrespective of the diet source probably due to inhibited absorption or very rapid elimination of OTC from circulation. Long withdrawal times were necessary in gilthead sea bream fed with both plant oil (312. h) and fish oil (239 h) to ensure consumer safety at winter temperatures. © 2010 Elsevier B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The effect of partial fish oil replacement (66%) by rapeseed, linseed and palm oil on the availability of oxytetracycline (OTC) was investigated in gilthead sea bream (Sparus aurata). Fish received a dosage of 75. mg OTC/kg for 5. days at 27 or 14°C water temperature. Tissues including blood and muscle plus skin were sampled on days 1, 3 and 5 during treatment and on days 6, 8, 12 and 16 post-treatment. At the high water temperature, OTC levels were undetectable in the tested tissues at all sampling points for both diets. At the winter temperature, plasma OTC concentrations were insignificantly higher in fish given plant oil-based diets compared to fish oil diets with maximum values of 1.03 and 0.73μg/ml respectively on day 6 for both groups. Similarly, OTC muscle plus skin levels were insignificantly lower in the fish oil compared to the plant oil group with respective values of 0.48μg/g on day 5 and 1.21μg/g on day 6. Overall, replacement of fish oil by plant oil sources showed no significant influence on OTC availability in gilthead sea bream at winter temperatures. The use of OTC should be discouraged at very high water temperatures in this species irrespective of the diet source probably due to inhibited absorption or very rapid elimination of OTC from circulation. Long withdrawal times were necessary in gilthead sea bream fed with both plant oil (312. h) and fish oil (239 h) to ensure consumer safety at winter temperatures. © 2010 Elsevier B.V. |
2010 |
Vatsos, I N; Kotzamanis, Y; Henry, M; Angelidis, P; Alexis, M N Monitoring stress in fish by applying image analysis to their skin mucous cells Journal Article European Journal of Histochemistry, 54 (2), pp. 107–111, 2010, ISSN: 1121760X. @article{vatsos_monitoring_2010, title = {Monitoring stress in fish by applying image analysis to their skin mucous cells}, author = {I N Vatsos and Y Kotzamanis and M Henry and P Angelidis and M N Alexis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77953160103&partnerID=40&md5=e164b9bb6870e5411efbe171878b3580}, issn = {1121760X}, year = {2010}, date = {2010-01-01}, journal = {European Journal of Histochemistry}, volume = {54}, number = {2}, pages = {107--111}, abstract = {Several authors have previously demonstrated that the number of the skin mucous cells of fish is affected by many stressors. In the present study, two experiments were conducted in order to examine the effects of two common environmental conditions on the morphology of skin of sea bass and particularly on the number and diameter of skin mucous cells. In the first experiment, two groups of sea bass (mean weight 155.6±10.3 g SD) were maintained in two different concentrations of nitrate, 100 and 700 ppm respectively, for 48 h, while a third group was used as control. In the second experiment, sea bass (initial mean weight 78.9±3.1 g SD) were divided into four groups and each group was maintained in a different level of oxygen for 9 weeks. The oxygen concentration in each group was: 3.6±0.2 ppm, 4.7±0.2 ppm, 6.2±0.2 ppm and 8.2±0.2 ppm. In both experiments the effects of the two environmental factors on the morphology of the fish skin were examined histologically and a software containing a visual basic script macro, allowing quantification of the skin mucous cells, was used to analyze the skin tissue sections. Concerning the overall morphology of the skin and the diameter of the skin mucous cells, no differences were noted in both experiments (P>0.05). It was demonstrated however, that fish maintained in the lowest oxygen level and fish maintained in the highest concentration of nitrate exhibited significantly increased number of mucous cells per skin area (mm 2). There is evidence that the enumeration of the skin mucous cells of fish can be used to monitor stress in fish. © I.N. Vatsos et al., 2010 Licensee PAGEPress, Italy.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Several authors have previously demonstrated that the number of the skin mucous cells of fish is affected by many stressors. In the present study, two experiments were conducted in order to examine the effects of two common environmental conditions on the morphology of skin of sea bass and particularly on the number and diameter of skin mucous cells. In the first experiment, two groups of sea bass (mean weight 155.6±10.3 g SD) were maintained in two different concentrations of nitrate, 100 and 700 ppm respectively, for 48 h, while a third group was used as control. In the second experiment, sea bass (initial mean weight 78.9±3.1 g SD) were divided into four groups and each group was maintained in a different level of oxygen for 9 weeks. The oxygen concentration in each group was: 3.6±0.2 ppm, 4.7±0.2 ppm, 6.2±0.2 ppm and 8.2±0.2 ppm. In both experiments the effects of the two environmental factors on the morphology of the fish skin were examined histologically and a software containing a visual basic script macro, allowing quantification of the skin mucous cells, was used to analyze the skin tissue sections. Concerning the overall morphology of the skin and the diameter of the skin mucous cells, no differences were noted in both experiments (P>0.05). It was demonstrated however, that fish maintained in the lowest oxygen level and fish maintained in the highest concentration of nitrate exhibited significantly increased number of mucous cells per skin area (mm 2). There is evidence that the enumeration of the skin mucous cells of fish can be used to monitor stress in fish. © I.N. Vatsos et al., 2010 Licensee PAGEPress, Italy. |
Fountoulaki, E; Henry, M; Rigos, G; Vasilaki, A; Mente, E; Sweetman, J; Nengas, I Evaluation of zinc supplementation in European sea bass (Dicentrarchus labrax) juvenile diets Journal Article Aquaculture Research, 41 , pp. e208 – e216, 2010. @article{fountoulaki_evaluation_2010, title = {Evaluation of zinc supplementation in European sea bass (Dicentrarchus labrax) juvenile diets}, author = {E Fountoulaki and M Henry and G Rigos and A Vasilaki and E Mente and J Sweetman and I Nengas}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77955955157&partnerID=40&md5=998c56a51dbd36e139c6f19395287c12}, doi = {10.1111/j.1365-2109.2010.02503.x}, year = {2010}, date = {2010-01-01}, journal = {Aquaculture Research}, volume = {41}, pages = {e208 -- e216}, abstract = {This study aimed to investigate the effect of increased zinc dietary levels on the growth performance, feed utilization, immune status and induced wound healing in European sea bass (Dicentrarchus labrax). Fish weighing 10 g were fed five diets containing organic zinc at 30, 70, 110 and 150 mg kg -1 diet and one inorganic zinc source (zinc oxide) at 150 mg kg -1 diet for a period of 12 weeks. No significant (P>0.05) differences were found in the growth performance parameters (final weight, specific growth rate), immunological indices tested (respiratory burst activity in whole blood) and the wound-healing process. Zinc accumulation in the skin, vertebrae and liver increased significantly (P<0.01) with increased dietary zinc levels but not in muscle. The highest zinc concentrations were obtained in skin tissue, followed by vertebrae, liver and muscle tissue. Using the concentration of zinc in skin as a response criterion, broken-line analysis showed that the supplementation of 148 mg organic Zn kg -1 diet seemed to be the optimum dietary zinc supplementation level for sea bass juveniles. © 2010 The Authors. Journal Compilation © 2010 Blackwell Publishing Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study aimed to investigate the effect of increased zinc dietary levels on the growth performance, feed utilization, immune status and induced wound healing in European sea bass (Dicentrarchus labrax). Fish weighing 10 g were fed five diets containing organic zinc at 30, 70, 110 and 150 mg kg -1 diet and one inorganic zinc source (zinc oxide) at 150 mg kg -1 diet for a period of 12 weeks. No significant (P>0.05) differences were found in the growth performance parameters (final weight, specific growth rate), immunological indices tested (respiratory burst activity in whole blood) and the wound-healing process. Zinc accumulation in the skin, vertebrae and liver increased significantly (P<0.01) with increased dietary zinc levels but not in muscle. The highest zinc concentrations were obtained in skin tissue, followed by vertebrae, liver and muscle tissue. Using the concentration of zinc in skin as a response criterion, broken-line analysis showed that the supplementation of 148 mg organic Zn kg -1 diet seemed to be the optimum dietary zinc supplementation level for sea bass juveniles. © 2010 The Authors. Journal Compilation © 2010 Blackwell Publishing Ltd. |
Rigos, G; Samartzis, A; Henry, M; Fountoulaki, E; Cotou, E; Sweetman, J; Davies, S; Nengas, I Effects of additive iron on growth, tissue distribution, haematology and immunology of gilthead sea bream, Sparus aurata Journal Article Aquaculture International, 18 , pp. 1093 – 1104, 2010. @article{rigos_effects_2010, title = {Effects of additive iron on growth, tissue distribution, haematology and immunology of gilthead sea bream, Sparus aurata}, author = {G Rigos and A Samartzis and M Henry and E Fountoulaki and E Cotou and J Sweetman and S Davies and I Nengas}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77957281268&partnerID=40&md5=cb00eeaf070421b4b6c73322441a8834}, doi = {10.1007/s10499-010-9326-7}, year = {2010}, date = {2010-01-01}, journal = {Aquaculture International}, volume = {18}, pages = {1093 -- 1104}, abstract = {The comparative effects of iron-supplemented levels on growth, tissue distribution, haematology and immunology of gilthead sea bream, Sparus aurata (2 g) were investigated, using four organic (50, 100, 200, 300 mg ORG/kg diet) and one inorganic iron source (200 INOR mg/kg diet). Fish were treated for 12 weeks with the experimental diets and maintained at a water temperature of 19-22°C. Growth (final weight and specific growth rate), tissue distribution (spleen, liver and muscle), haematological parameters (red blood cells, haematocrit, haemoglobin and mean corpuscular haemoglobin concentration) and non-specific immune indexes (respiratory burst activity and antibacterial activity of serum) were analysed. No significant differences were found in growth and iron tissue distribution among the tested groups. Red blood cell counting was statistically higher in fish given 50 ORG, 100 ORG, 200 ORG and 200 INOR feeds. However, haematocrit, haemoglobin and mean corpuscular haemoglobin concentration were not significantly affected by increasing dietary iron. Fish receiving the 100 ORG diet had the best performance with respect to the respiratory burst activity and significantly higher values for antibacterial activity of serum were obtained in fish fed with the 300 ORG diet. The present findings provided no clear evidence of the optimum iron concentration. However, there was adequate indication that iron supplementation enhanced the performance of gilthead sea bream, mainly from a haematological and immunological point of view. © 2010 Springer Science+Business Media B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The comparative effects of iron-supplemented levels on growth, tissue distribution, haematology and immunology of gilthead sea bream, Sparus aurata (2 g) were investigated, using four organic (50, 100, 200, 300 mg ORG/kg diet) and one inorganic iron source (200 INOR mg/kg diet). Fish were treated for 12 weeks with the experimental diets and maintained at a water temperature of 19-22°C. Growth (final weight and specific growth rate), tissue distribution (spleen, liver and muscle), haematological parameters (red blood cells, haematocrit, haemoglobin and mean corpuscular haemoglobin concentration) and non-specific immune indexes (respiratory burst activity and antibacterial activity of serum) were analysed. No significant differences were found in growth and iron tissue distribution among the tested groups. Red blood cell counting was statistically higher in fish given 50 ORG, 100 ORG, 200 ORG and 200 INOR feeds. However, haematocrit, haemoglobin and mean corpuscular haemoglobin concentration were not significantly affected by increasing dietary iron. Fish receiving the 100 ORG diet had the best performance with respect to the respiratory burst activity and significantly higher values for antibacterial activity of serum were obtained in fish fed with the 300 ORG diet. The present findings provided no clear evidence of the optimum iron concentration. However, there was adequate indication that iron supplementation enhanced the performance of gilthead sea bream, mainly from a haematological and immunological point of view. © 2010 Springer Science+Business Media B.V. |
2009 |
Adamidou, S; Nengas, I; Henry, M; Grigorakis, K; Rigos, G; Nikolopoulou, D; Kotzamanis, Y; Bell, G J; Jauncey, K Aquaculture, 293 (3-4), pp. 263–271, 2009, ISSN: 00448486. @article{adamidou_growth_2009, title = {Growth, feed utilization, health and organoleptic characteristics of European seabass (Dicentrarchus labrax) fed extruded diets including low and high levels of three different legumes}, author = {S Adamidou and I Nengas and M Henry and K Grigorakis and G Rigos and D Nikolopoulou and Y Kotzamanis and G J Bell and K Jauncey}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-67649088041&doi=10.1016%2fj.aquaculture.2009.04.045&partnerID=40&md5=dfaf34a8796e08dbe50d7099ac4f3f40}, doi = {10.1016/j.aquaculture.2009.04.045}, issn = {00448486}, year = {2009}, date = {2009-01-01}, journal = {Aquaculture}, volume = {293}, number = {3-4}, pages = {263--271}, abstract = {Three legumes [field peas (P), chickpeas (CP) and faba beans (B)] were evaluated at two inclusion levels; 170 (L) and 350 g kg- 1 (H) in a 14-week experiment with triplicate groups of 97.9 ± 6.1 g European seabass. A control diet included wheat meal, fish meal (FM) and a mixture of plant ingredients as protein sources. Diets, processed in a twin-screw extruder, were isonitrogenous and isoenergetic. All experimental diets improved growth compared to the control. Weight gain, feed intake, FCR and SGR were improved for fish fed diet CPL, while fish fed diet CPH gave higher FCR. Protein and starch digestibility were highest for the control diet, while fish fed diets CPL and PL showed significantly lower protein ADCs and lower starch ADC (P ≤ 0.05) for diet PH. Among H diets, significantly better fillet yield was found for fish fed diet PH, but no other differences were found in seabass fillet organoleptic characteristics. Serum glucose and cholesterol were elevated in fish fed CP diets, while protein and triacylglycerols did not show significant differences among treatments. Fish fed the test diets showed no significant evidence of either immunosuppression or immunostimulation. Histology of liver, spleen, kidney and foregut revealed no pathological abnormalities. Field peas, chickpeas and faba beans can be included in European seabass diets up to 350 g kg- 1 substituting for wheat with no negative effects on growth performance, carcass composition or organoleptic characteristics. © 2009 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Three legumes [field peas (P), chickpeas (CP) and faba beans (B)] were evaluated at two inclusion levels; 170 (L) and 350 g kg- 1 (H) in a 14-week experiment with triplicate groups of 97.9 ± 6.1 g European seabass. A control diet included wheat meal, fish meal (FM) and a mixture of plant ingredients as protein sources. Diets, processed in a twin-screw extruder, were isonitrogenous and isoenergetic. All experimental diets improved growth compared to the control. Weight gain, feed intake, FCR and SGR were improved for fish fed diet CPL, while fish fed diet CPH gave higher FCR. Protein and starch digestibility were highest for the control diet, while fish fed diets CPL and PL showed significantly lower protein ADCs and lower starch ADC (P ≤ 0.05) for diet PH. Among H diets, significantly better fillet yield was found for fish fed diet PH, but no other differences were found in seabass fillet organoleptic characteristics. Serum glucose and cholesterol were elevated in fish fed CP diets, while protein and triacylglycerols did not show significant differences among treatments. Fish fed the test diets showed no significant evidence of either immunosuppression or immunostimulation. Histology of liver, spleen, kidney and foregut revealed no pathological abnormalities. Field peas, chickpeas and faba beans can be included in European seabass diets up to 350 g kg- 1 substituting for wheat with no negative effects on growth performance, carcass composition or organoleptic characteristics. © 2009 Elsevier B.V. All rights reserved. |
Henry, M; Alexis, M N; Fountoulaki, E; Nengas, I; Rigos, G Effects of a natural parasitical infection (Lernanthropus kroyeri) on the immune system of European sea bass, Dicentrarchus labrax L Journal Article Parasite Immunology, 31 , pp. 729 – 740, 2009. @article{henry_effects_2009, title = {Effects of a natural parasitical infection (Lernanthropus kroyeri) on the immune system of European sea bass, Dicentrarchus labrax L}, author = {M Henry and M N Alexis and E Fountoulaki and I Nengas and G Rigos}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-71049186396&partnerID=40&md5=856f23a4caa21fd8ac2308babc61d401}, year = {2009}, date = {2009-01-01}, journal = {Parasite Immunology}, volume = {31}, pages = {729 -- 740}, abstract = {The immune response of European sea bass, Dicentrarchus labrax, to a natural infection by the copepod parasite Lernanthropus kroyeri was evaluated for the first time in vivo. The results clearly demonstrated the triggering of the fish immune system by the parasite. Lysozyme activity and alternative complement pathway were involved in the early action against the parasitical infection, whilst classical complement and respiratory burst (RB) activity took over in the later stages of infection. It was hypothesized that the levels of alternative and classical complement and RB stimulation indexes may determine the resistance capacity of the fish to the parasite. It is not clear how parasites can survive despite the strong immunological arsenal deployed by the fish. The continual increase of prevalence and severity of parasite infection suggested that the parasite’s mechanism of evasion of the immune system was extremely successful. The contrasting decrease in the negative effects of parasites on the fish health (such as gills anaemia) suggested that an equilibrium between the parasites and their hosts was reached in chronic infection. These dynamic interactions between parasites and fish hosts were probably the main determinant of host specificity. © 2009 Hellenic Centre for Marine Research.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The immune response of European sea bass, Dicentrarchus labrax, to a natural infection by the copepod parasite Lernanthropus kroyeri was evaluated for the first time in vivo. The results clearly demonstrated the triggering of the fish immune system by the parasite. Lysozyme activity and alternative complement pathway were involved in the early action against the parasitical infection, whilst classical complement and respiratory burst (RB) activity took over in the later stages of infection. It was hypothesized that the levels of alternative and classical complement and RB stimulation indexes may determine the resistance capacity of the fish to the parasite. It is not clear how parasites can survive despite the strong immunological arsenal deployed by the fish. The continual increase of prevalence and severity of parasite infection suggested that the parasite’s mechanism of evasion of the immune system was extremely successful. The contrasting decrease in the negative effects of parasites on the fish health (such as gills anaemia) suggested that an equilibrium between the parasites and their hosts was reached in chronic infection. These dynamic interactions between parasites and fish hosts were probably the main determinant of host specificity. © 2009 Hellenic Centre for Marine Research. |
Henry, M; Alexis, M N Effects of in vitro lactoferricin and lactoferrin on the head kidney cells of European sea bass (Dicentrarchus labrax, L.) Journal Article Veterinary Immunology and Immunopathology, 130 , pp. 236 – 242, 2009. @article{henry_effects_2009-1, title = {Effects of in vitro lactoferricin and lactoferrin on the head kidney cells of European sea bass (Dicentrarchus labrax, L.)}, author = {M Henry and M N Alexis}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-67649946810&partnerID=40&md5=5c681def25ec82185a873d50196e1731}, doi = {10.1016/j.vetimm.2009.02.014}, year = {2009}, date = {2009-01-01}, journal = {Veterinary Immunology and Immunopathology}, volume = {130}, pages = {236 -- 242}, abstract = {Antimicrobial, anti-inflammatory and immunomodulating properties of lactoferrin have been demonstrated in mammals and in fish. However, in vivo, lactoferrin is digested by gastric pepsin treatment into the N-terminal derived peptide named lactoferricin. This has been so far overlooked in fish in vitro studies. The aim of the present study was to assess in vitro the effects of both lactoferricin and lactoferrin on the head kidney cells of European sea bass (Dicentrarchus labrax, L.) in order to determine their potential as dietary additives and to get some insight into their mode of action. In vitro lactoferricin decreased significantly the chemiluminescent response of head kidney cells but did not affect the zymosan-triggered chemiluminescence activity. On the other hand, a high concentration of lactoferrin directly stimulated chemiluminescence but reduced the zymosan-triggered chemiluminescence. The bactericidal activity of head kidney cells was also significantly diminished by pre-incubation with lactoferrin in a dose-dependent manner. Although no significant effect of lactoferricin or lactoferrin was evidenced on head kidney cellular viability, absent or negative effect on the priming of respiratory burst activity suggested that care should be taken when using lactoferrin in the diet of sea bass and high doses should be avoided. Hypotheses about the mechanisms of action of lactoferricin and lactoferrin are presented. © 2009 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Antimicrobial, anti-inflammatory and immunomodulating properties of lactoferrin have been demonstrated in mammals and in fish. However, in vivo, lactoferrin is digested by gastric pepsin treatment into the N-terminal derived peptide named lactoferricin. This has been so far overlooked in fish in vitro studies. The aim of the present study was to assess in vitro the effects of both lactoferricin and lactoferrin on the head kidney cells of European sea bass (Dicentrarchus labrax, L.) in order to determine their potential as dietary additives and to get some insight into their mode of action. In vitro lactoferricin decreased significantly the chemiluminescent response of head kidney cells but did not affect the zymosan-triggered chemiluminescence activity. On the other hand, a high concentration of lactoferrin directly stimulated chemiluminescence but reduced the zymosan-triggered chemiluminescence. The bactericidal activity of head kidney cells was also significantly diminished by pre-incubation with lactoferrin in a dose-dependent manner. Although no significant effect of lactoferricin or lactoferrin was evidenced on head kidney cellular viability, absent or negative effect on the priming of respiratory burst activity suggested that care should be taken when using lactoferrin in the diet of sea bass and high doses should be avoided. Hypotheses about the mechanisms of action of lactoferricin and lactoferrin are presented. © 2009 Elsevier B.V. All rights reserved. |
Vatsos, I; Kotzamanis, Y; Vectesi, D; Henry, Morgane; Angelidis, P Short-term of European sea bass (Dicentrarchus labrax) to nitrate. Effects on skin morphology and skin mucous cells. Journal Article Sc. Annals of DDI, 15 , pp. 105–108, 2009. @article{vatsos_short-term_2009, title = {Short-term of European sea bass (Dicentrarchus labrax) to nitrate. Effects on skin morphology and skin mucous cells.}, author = {I Vatsos and Y Kotzamanis and D Vectesi and Morgane Henry and P Angelidis}, year = {2009}, date = {2009-01-01}, journal = {Sc. Annals of DDI}, volume = {15}, pages = {105--108}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2000 |
Henry, Morgane A; Secombes, Christopher J The A-layer influences the susceptibility of Aeromonas salmonicida to antibacterial peptides Journal Article Fish & Shellfish Immunology, 10 (7), pp. 637–642, 2000, ISSN: 10504648. @article{henry_-layer_2000, title = {The A-layer influences the susceptibility of Aeromonas salmonicida to antibacterial peptides}, author = {Morgane A Henry and Christopher J Secombes}, url = {https://linkinghub.elsevier.com/retrieve/pii/S1050464800902882}, doi = {10.1006/fsim.2000.0288}, issn = {10504648}, year = {2000}, date = {2000-10-01}, urldate = {2020-08-18}, journal = {Fish & Shellfish Immunology}, volume = {10}, number = {7}, pages = {637--642}, keywords = {}, pubstate = {published}, tppubtype = {article} } |