2020
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Polymenakou, Paraskevi N; Mandalakis, Manolis; Macheras, Michalis; Oulas, Anastasis; Kristoffersen, Jon Bent; Christakis, Christos A; Terzoglou, Vasso; Stavroulaki, Melanthia High genetic diversity and variability of microbial communities in near-surface atmosphere of Crete island, Greece Journal Article Aerobiologia, 36 (3), pp. 341–353, 2020, ISSN: 0393-5965, 1573-3025. Links | BibTeX @article{polymenakou_high_2020,
title = {High genetic diversity and variability of microbial communities in near-surface atmosphere of Crete island, Greece},
author = {Paraskevi N Polymenakou and Manolis Mandalakis and Michalis Macheras and Anastasis Oulas and Jon Bent Kristoffersen and Christos A Christakis and Vasso Terzoglou and Melanthia Stavroulaki},
url = {http://link.springer.com/10.1007/s10453-020-09636-w},
doi = {10.1007/s10453-020-09636-w},
issn = {0393-5965, 1573-3025},
year = {2020},
date = {2020-09-01},
urldate = {2020-08-31},
journal = {Aerobiologia},
volume = {36},
number = {3},
pages = {341--353},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
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2009
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Sarropoulou, E; Sepulcre, P; Poisa-Beiro, L; Mulero, V; Meseguer, J; Figueras, A; Novoa, B; Terzoglou, V; Reinhardt, R; Magoulas, A; Kotoulas, G Profiling of infection specific mRNA transcripts of the European seabass Dicentrarchus labrax Journal Article BMC Genomics, 10 , 2009, ISSN: 14712164. Abstract | Links | BibTeX @article{sarropoulou_profiling_2009,
title = {Profiling of infection specific mRNA transcripts of the European seabass Dicentrarchus labrax},
author = {E Sarropoulou and P Sepulcre and L Poisa-Beiro and V Mulero and J Meseguer and A Figueras and B Novoa and V Terzoglou and R Reinhardt and A Magoulas and G Kotoulas},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-65449174874&doi=10.1186%2f1471-2164-10-157&partnerID=40&md5=fc9c5760823ecfa4f4b04e26d52e3c4c},
doi = {10.1186/1471-2164-10-157},
issn = {14712164},
year = {2009},
date = {2009-01-01},
journal = {BMC Genomics},
volume = {10},
abstract = {Background: The European seabass (Dicentrarchus labrax), one of the most extensively cultured species in European aquaculture productions, is, along with the gilthead sea bream (Sparus aurata), a prospective model species for the Perciformes which includes several other commercially important species. Massive mortalities may be caused by bacterial or viral infections in intensive aquaculture production. Revealing transcripts involved in immune response and studying their relative expression enhances the understanding of the immune response mechanism and consequently also the creation of vaccines. The analysis of expressed sequence tags (EST) is an efficient and easy approach for gene discovery, comparative genomics and for examining gene expression in specific tissues in a qualitative and quantitative way. Results: Here we describe the construction, analysis and comparison of a total of ten cDNA libraries, six from different tissues infected with V. anguillarum (liver, spleen, head kidney, gill, peritoneal exudates and intestine) and four cDNA libraries from different tissues infected with Nodavirus (liver, spleen, head kidney and brain). In total 9605 sequences representing 3075 (32%) unique sequences (set of sequences obtained after clustering) were obtained and analysed. Among the sequences several immune-related proteins were identified for the first time in the order of Perciformes as well as in Teleostei. Conclusion: The present study provides new information to the Gene Index of seabass. It gives a unigene set that will make a significant contribution to functional genomic studies and to studies of differential gene expression in relation to the immune system. In addition some of the potentially interesting genes identified by in silico analysis and confirmed by real-time PCR are putative biomarkers for bacterial and viral infections in fish. © 2009 Sarropoulou et al; licensee BioMed Central Ltd.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Background: The European seabass (Dicentrarchus labrax), one of the most extensively cultured species in European aquaculture productions, is, along with the gilthead sea bream (Sparus aurata), a prospective model species for the Perciformes which includes several other commercially important species. Massive mortalities may be caused by bacterial or viral infections in intensive aquaculture production. Revealing transcripts involved in immune response and studying their relative expression enhances the understanding of the immune response mechanism and consequently also the creation of vaccines. The analysis of expressed sequence tags (EST) is an efficient and easy approach for gene discovery, comparative genomics and for examining gene expression in specific tissues in a qualitative and quantitative way. Results: Here we describe the construction, analysis and comparison of a total of ten cDNA libraries, six from different tissues infected with V. anguillarum (liver, spleen, head kidney, gill, peritoneal exudates and intestine) and four cDNA libraries from different tissues infected with Nodavirus (liver, spleen, head kidney and brain). In total 9605 sequences representing 3075 (32%) unique sequences (set of sequences obtained after clustering) were obtained and analysed. Among the sequences several immune-related proteins were identified for the first time in the order of Perciformes as well as in Teleostei. Conclusion: The present study provides new information to the Gene Index of seabass. It gives a unigene set that will make a significant contribution to functional genomic studies and to studies of differential gene expression in relation to the immune system. In addition some of the potentially interesting genes identified by in silico analysis and confirmed by real-time PCR are putative biomarkers for bacterial and viral infections in fish. © 2009 Sarropoulou et al; licensee BioMed Central Ltd. |
2005
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Brown, R C; Tsalavouta, M; Terzoglou, V; Magoulas, A; McAndrew, B J Additional microsatellites for Sparus aurata and cross-species amplification within the Sparidae family Journal Article Molecular Ecology Notes, 5 , pp. 605 – 607, 2005. Abstract | Links | BibTeX @article{brown_additional_2005,
title = {Additional microsatellites for Sparus aurata and cross-species amplification within the Sparidae family},
author = {R C Brown and M Tsalavouta and V Terzoglou and A Magoulas and B J McAndrew},
url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-28344455572&partnerID=40&md5=7f56611349fb1cbe98f6e3fbe1c0e1a7},
doi = {10.1111/j.1471-8286.2005.01007.x},
year = {2005},
date = {2005-01-01},
journal = {Molecular Ecology Notes},
volume = {5},
pages = {605 -- 607},
abstract = {Six new microsatellite loci were isolated and characterized in 32 individuals from a farm population of gilthead seabream (Sparus aurata). Expected heterozygosity at all loci was high, ranging from 0.835 to 0.958 with between 10 and 27 alleles per locus. A multiplex polymerase chain reaction protocol was developed using four of the loci. Cross-species amplification of the loci was tested in six species of the Sparidae family and four loci were successfully amplified in two or more related species. © 2005 Blackwell Publishing Ltd.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Six new microsatellite loci were isolated and characterized in 32 individuals from a farm population of gilthead seabream (Sparus aurata). Expected heterozygosity at all loci was high, ranging from 0.835 to 0.958 with between 10 and 27 alleles per locus. A multiplex polymerase chain reaction protocol was developed using four of the loci. Cross-species amplification of the loci was tested in six species of the Sparidae family and four loci were successfully amplified in two or more related species. © 2005 Blackwell Publishing Ltd. |
2004
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Kasapidis, P; Planes, S; Laurent, V; Quinta, R; Teia, A; Lenfant, R; Darivianakis, S; Terzoglou, V; Kotoulas, G; Magoulas, A Stock discrimination and temporal and spatial genetic variation of sardine (Sardina pilchardus) in northeastern Atlantic, with a combined analysis of nuclear (microsatellites and allozymes) and mitochondrial DNA markers. Inproceedings Kasapidis, P (Ed.): 2004 ICES Annual Science Conference, 2004. BibTeX @inproceedings{kasapidis_stock_2004,
title = {Stock discrimination and temporal and spatial genetic variation of sardine (Sardina pilchardus) in northeastern Atlantic, with a combined analysis of nuclear (microsatellites and allozymes) and mitochondrial DNA markers.},
author = {P Kasapidis and S Planes and V Laurent and R Quinta and A Teia and R Lenfant and S Darivianakis and V Terzoglou and G Kotoulas and A Magoulas},
editor = {P Kasapidis},
year = {2004},
date = {2004-09-01},
booktitle = {2004 ICES Annual Science Conference},
keywords = {},
pubstate = {published},
tppubtype = {inproceedings}
}
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1998
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Magoulas, A; Gjetvaj, B; Terzoglou, V; Zouros, E Three polymorphic microsatellites in the Japanese oyster, Crassostrea gigas (Thunberg) Journal Article Animal Genetics, 29 , pp. 69 – 70, 1998. Links | BibTeX @article{magoulas_three_1998,
title = {Three polymorphic microsatellites in the Japanese oyster, Crassostrea gigas (Thunberg)},
author = {A Magoulas and B Gjetvaj and V Terzoglou and E Zouros},
url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0031869608&partnerID=40&md5=d61c90542e845be7feace22b67b93902},
year = {1998},
date = {1998-01-01},
journal = {Animal Genetics},
volume = {29},
pages = {69 -- 70},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
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