This user account status is Approved
2026
Henry, Morgane A.; Fountoulaki, Eleni; Mastoraki, Maria; Chronopoulos, Petros; Kogiannou, Dimitra; Vasilaki, Antigoni; Nikoloudaki, Chrisanthi; Theillier, Eloise; Chatteleyn, Matteo; Karapanagiotidis, Ioannis T.
In: Fishes, vol. 11, no. 8, pp. 444, 2026, ISSN: 2410-3888.
@article{henry_effects_2026,
title = {Effects of Fish Oil and Microalgae Finishing Diets on Fillet Fatty Acid Recovery, Liver and Intestine Histology, and Innate Immune Parameters of European Sea Bass (\textit{Dicentrarchus labrax}) Fed with Rapeseed Oil},
author = {Morgane A. Henry and Eleni Fountoulaki and Maria Mastoraki and Petros Chronopoulos and Dimitra Kogiannou and Antigoni Vasilaki and Chrisanthi Nikoloudaki and Eloise Theillier and Matteo Chatteleyn and Ioannis T. Karapanagiotidis},
url = {https://www.mdpi.com/2410-3888/11/8/444
/wp-content/uploads/2026/07/2026-Henry-FISHES-32.pdf},
doi = {10.3390/fishes11080444},
issn = {2410-3888},
year = {2026},
date = {2026-07-30},
urldate = {2026-07-01},
journal = {Fishes},
volume = {11},
number = {8},
pages = {444},
abstract = {This study evaluated the use of finishing diets to reduce fish oil (FO) inclusion in European sea bass feeds while preserving fillet quality and n-3 fatty acid content. Fish (197 g) were fed for 25 weeks with a control FO diet, based on 9% FO and 6% plant oil, or diets where 6% FO was replaced by rapeseed oil (RO) or microalgae (AO; Nannochloropsis sp. and Schizochytrium sp.). After 13 weeks, 2 groups of fish fed the RO diet were switched to the FO or AO finishing diets for the final 12 weeks. Growth, feed efficiency, body composition, and health were unaffected by dietary treatments. However, long-term RO feeding reduced eicosapentaenoic acid (EPA) and docosahexanoic acid (DHA) and increased monounsaturated and n-6 fatty acids compared to fish fed AO or FO. A 12-wk FO finishing diets shifted morphometric traits, fatty acid profiles, liver histology, and overall fish quality of fish previously fed with RO toward those of fish continuously fed FO, whereas the AO finishing diet produced a smaller shift. Longer finishing periods may further enhance fillet quality when using microalgae-based oils.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
This study evaluated the use of finishing diets to reduce fish oil (FO) inclusion in European sea bass feeds while preserving fillet quality and n-3 fatty acid content. Fish (197 g) were fed for 25 weeks with a control FO diet, based on 9% FO and 6% plant oil, or diets where 6% FO was replaced by rapeseed oil (RO) or microalgae (AO; Nannochloropsis sp. and Schizochytrium sp.). After 13 weeks, 2 groups of fish fed the RO diet were switched to the FO or AO finishing diets for the final 12 weeks. Growth, feed efficiency, body composition, and health were unaffected by dietary treatments. However, long-term RO feeding reduced eicosapentaenoic acid (EPA) and docosahexanoic acid (DHA) and increased monounsaturated and n-6 fatty acids compared to fish fed AO or FO. A 12-wk FO finishing diets shifted morphometric traits, fatty acid profiles, liver histology, and overall fish quality of fish previously fed with RO toward those of fish continuously fed FO, whereas the AO finishing diet produced a smaller shift. Longer finishing periods may further enhance fillet quality when using microalgae-based oils.
Debailleul, Gatien; Gatouillat, Hugo; Chatzimpalasis, Dimitrios; Chatteleyn, Matteo; Chronopoulos, Petros; Nikoloudaki, Chrisanthi; Vasilaki, Antigoni; Kogiannou, Dimitra; Henry, Morgane
Antibacterial Activity of Extracts from Seven Insect Species Against Mediterranean Marine Aquaculture Bacterial Pathogens Journal Article
In: Fishes, vol. 11, no. 7, pp. 389, 2026, ISSN: 2410-3888.
@article{debailleul_antibacterial_2026,
title = {Antibacterial Activity of Extracts from Seven Insect Species Against Mediterranean Marine Aquaculture Bacterial Pathogens},
author = {Gatien Debailleul and Hugo Gatouillat and Dimitrios Chatzimpalasis and Matteo Chatteleyn and Petros Chronopoulos and Chrisanthi Nikoloudaki and Antigoni Vasilaki and Dimitra Kogiannou and Morgane Henry},
url = {https://www.mdpi.com/2410-3888/11/7/389
/wp-content/uploads/2026/07/2026-Debailleu-FISHES-28.pdf},
doi = {10.3390/fishes11070389},
issn = {2410-3888},
year = {2026},
date = {2026-07-13},
urldate = {2026-06-01},
journal = {Fishes},
volume = {11},
number = {7},
pages = {389},
abstract = {Beyond their role as fishmeal replacements in aquafeeds, insects may enhance fish resistance to microbial diseases and improve feed shelf life through the presence of antibacterial compounds. This study evaluated the antibacterial activity of extracts from silkworm pupae (Bombyx mori, BM), larval meals of black soldier fly (Hermetia illucens, HI), mealworm (Tenebrio molitor, TM), and superworm (Zophobas morio, ZM), and adults of house cricket (Acheta domesticus, AD), two-spotted cricket (Gryllus bimaculatus, GB) and weaver ant (Oecophylla smaragdina, OS). The extracts were tested against ten Gram-negative marine bacterial pathogens associated with aquaculture disease outbreaks, as well as the Gram-positive bacterium, Bacillus thuringiensis. The antibacterial activity of aqueous and solvent-soluble extracts was assessed using agar diffusion assays, turbidimetric growth kinetics, and MTT metabolic assays. Several insect extracts exhibited moderate to strong antibacterial activity. In particular, the aqueous extracts of BM and GB, the methanolic extract of HI, and the isopropanolic extracts of HI and OS were effective against multiple bacterial strains, including strains with reduced susceptibility to the reference antibiotics bacitracin, oxolinic acid, and streptomycin. These findings highlight the potential of HI, GB and especially OS as functional ingredients or feed additives for aquaculture.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Beyond their role as fishmeal replacements in aquafeeds, insects may enhance fish resistance to microbial diseases and improve feed shelf life through the presence of antibacterial compounds. This study evaluated the antibacterial activity of extracts from silkworm pupae (Bombyx mori, BM), larval meals of black soldier fly (Hermetia illucens, HI), mealworm (Tenebrio molitor, TM), and superworm (Zophobas morio, ZM), and adults of house cricket (Acheta domesticus, AD), two-spotted cricket (Gryllus bimaculatus, GB) and weaver ant (Oecophylla smaragdina, OS). The extracts were tested against ten Gram-negative marine bacterial pathogens associated with aquaculture disease outbreaks, as well as the Gram-positive bacterium, Bacillus thuringiensis. The antibacterial activity of aqueous and solvent-soluble extracts was assessed using agar diffusion assays, turbidimetric growth kinetics, and MTT metabolic assays. Several insect extracts exhibited moderate to strong antibacterial activity. In particular, the aqueous extracts of BM and GB, the methanolic extract of HI, and the isopropanolic extracts of HI and OS were effective against multiple bacterial strains, including strains with reduced susceptibility to the reference antibiotics bacitracin, oxolinic acid, and streptomycin. These findings highlight the potential of HI, GB and especially OS as functional ingredients or feed additives for aquaculture.
2025
Vasilaki, Antigoni; Nengas, Ioannis; Kogiannou, Dimitra; Henry, Morgane; Nikoloudaki, Chrysanthi; Chronopoulos, Petros; Berillis, Panagiotis; Golomazou, Eleni; Fountoulaki, Eleni; Karapanagiotidis, Ioannis T.; Mente, Elena
In: Aquaculture Reports, vol. 45, pp. 103135, 2025, ISSN: 23525134.
@article{vasilaki_evaluation_2025,
title = {Evaluation of the nutritional value of processed lupin meal (Lupinus albus) with exogenous enzymes, as feed ingredient in European sea bass (Dicentrarchus labrax) aquafeeds},
author = {Antigoni Vasilaki and Ioannis Nengas and Dimitra Kogiannou and Morgane Henry and Chrysanthi Nikoloudaki and Petros Chronopoulos and Panagiotis Berillis and Eleni Golomazou and Eleni Fountoulaki and Ioannis T. Karapanagiotidis and Elena Mente},
url = {https://linkinghub.elsevier.com/retrieve/pii/S2352513425005216
/wp-content/uploads/2025/11/2025-Vasilaki-Aqua-Rep-65.pdf},
doi = {10.1016/j.aqrep.2025.103135},
issn = {23525134},
year = {2025},
date = {2025-11-06},
urldate = {2025-12-01},
journal = {Aquaculture Reports},
volume = {45},
pages = {103135},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Tampou, Anna; Kousoulaki, Katerina; Vasilaki, Antigoni; Vlahos, Nikolaos; Nikouli, Eleni; Panteli, Nikolas; Feidantsis, Konstantinos; Kormas, Konstantinos; Andreopoulou, Styliani; Karapanagiotidis, Ioannis T; Berillis, Panagiotis; Nengas, Ioannis; Antonopoulou, Efthimia; Mente, Eleni
Growth Performance of Gilthead Sea Bream ( Sparus aurata ) Fed Low Fish Meal Diets With an Innovative Mixture of Low Trophic Ingredients Journal Article
In: Aquaculture Nutrition, vol. 2025, no. 1, pp. 7504207, 2025, ISSN: 1353-5773, 1365-2095.
@article{tampou_growth_2025,
title = {Growth Performance of Gilthead Sea Bream ( \textit{Sparus aurata} ) Fed Low Fish Meal Diets With an Innovative Mixture of Low Trophic Ingredients},
author = {Anna Tampou and Katerina Kousoulaki and Antigoni Vasilaki and Nikolaos Vlahos and Eleni Nikouli and Nikolas Panteli and Konstantinos Feidantsis and Konstantinos Kormas and Styliani Andreopoulou and Ioannis T Karapanagiotidis and Panagiotis Berillis and Ioannis Nengas and Efthimia Antonopoulou and Eleni Mente},
editor = {Noah Esmaeili},
url = {https://onlinelibrary.wiley.com/doi/10.1155/anu/7504207
/wp-content/uploads/2025/09/2025-Tampou-Aqua-Nutr-48.pdf},
doi = {10.1155/anu/7504207},
issn = {1353-5773, 1365-2095},
year = {2025},
date = {2025-09-11},
urldate = {2025-09-12},
journal = {Aquaculture Nutrition},
volume = {2025},
number = {1},
pages = {7504207},
abstract = {This study examined the growth performance, cellular signaling, and gut microbiome of gilthead sea bream (
Sparus aurata
) fed four diets using low‐trophic‐level ingredients for 65 days. Control (C) diet contained fish meal (FM) as the main protein source and fish oil (FO) as a lipid source. In the 0%FMFO diet all FM and FO present in the C diet was replaced with a combination of microalgae, insect meal (IM), and tunicate meal (TM). IM and TM diets were formulated to contain 20% of the novel protein ingredients, replacing 68.09% and 45.91% of FM in diet C, respectively. Compared to diet C, feed utilization and growth performance of
S
.
aurata
fed 0%FMFO diet was not different (
p
textgreater 0.05) and TM diet had a significantly lower (
p
textless 0.05) specific growth rate (SGR), higher (
p
textless 0.05) feed conversion ratio (FCR) and decreased fillet lipid content (
p
textless 0.05). In liver and foregut of fish fed TM diet the activity of lactate dehydrogenase (L‐LDH) significant increased (
p
textless 0.05), indicating higher glycolytic potential, whereas the decrease in Hsp70, Hsp90, AMPK, and p38 MAPK may indicate reduced stress response. Fish midgut microbiome included beneficial taxa for the host. The results suggested that the mixture of algae, insect, and TM could replace all FM and FO in gilthead sea bream diets without affecting the fish growth performance.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
This study examined the growth performance, cellular signaling, and gut microbiome of gilthead sea bream (
Sparus aurata
) fed four diets using low‐trophic‐level ingredients for 65 days. Control (C) diet contained fish meal (FM) as the main protein source and fish oil (FO) as a lipid source. In the 0%FMFO diet all FM and FO present in the C diet was replaced with a combination of microalgae, insect meal (IM), and tunicate meal (TM). IM and TM diets were formulated to contain 20% of the novel protein ingredients, replacing 68.09% and 45.91% of FM in diet C, respectively. Compared to diet C, feed utilization and growth performance of
S
.
aurata
fed 0%FMFO diet was not different (
p
textgreater 0.05) and TM diet had a significantly lower (
p
textless 0.05) specific growth rate (SGR), higher (
p
textless 0.05) feed conversion ratio (FCR) and decreased fillet lipid content (
p
textless 0.05). In liver and foregut of fish fed TM diet the activity of lactate dehydrogenase (L‐LDH) significant increased (
p
textless 0.05), indicating higher glycolytic potential, whereas the decrease in Hsp70, Hsp90, AMPK, and p38 MAPK may indicate reduced stress response. Fish midgut microbiome included beneficial taxa for the host. The results suggested that the mixture of algae, insect, and TM could replace all FM and FO in gilthead sea bream diets without affecting the fish growth performance.
Sparus aurata
) fed four diets using low‐trophic‐level ingredients for 65 days. Control (C) diet contained fish meal (FM) as the main protein source and fish oil (FO) as a lipid source. In the 0%FMFO diet all FM and FO present in the C diet was replaced with a combination of microalgae, insect meal (IM), and tunicate meal (TM). IM and TM diets were formulated to contain 20% of the novel protein ingredients, replacing 68.09% and 45.91% of FM in diet C, respectively. Compared to diet C, feed utilization and growth performance of
S
.
aurata
fed 0%FMFO diet was not different (
p
textgreater 0.05) and TM diet had a significantly lower (
p
textless 0.05) specific growth rate (SGR), higher (
p
textless 0.05) feed conversion ratio (FCR) and decreased fillet lipid content (
p
textless 0.05). In liver and foregut of fish fed TM diet the activity of lactate dehydrogenase (L‐LDH) significant increased (
p
textless 0.05), indicating higher glycolytic potential, whereas the decrease in Hsp70, Hsp90, AMPK, and p38 MAPK may indicate reduced stress response. Fish midgut microbiome included beneficial taxa for the host. The results suggested that the mixture of algae, insect, and TM could replace all FM and FO in gilthead sea bream diets without affecting the fish growth performance.
Vasilaki, Antigoni; Nengas, Ioannis; Fountoulaki, Eleni; Henry, Morgane; Kogiannou, Dimitra; Nikoloudaki, Chrysanthi; Chronopoulos, Petros; Karapanagiotidis, Ioannis T; Mente, Elena
In: Aquaculture, vol. 609, pp. 742820, 2025, ISSN: 0044-8486.
@article{vasilaki_nutritional_2025,
title = {Nutritional enhancement of lupin meal (\textit{Lupinus albus}), through fermentation with textitSaccharomyces cerevisiae, as plant protein ingredient in aquafeeds for the European sea bass (\textit{Dicentrarchus labrax}).},
author = {Antigoni Vasilaki and Ioannis Nengas and Eleni Fountoulaki and Morgane Henry and Dimitra Kogiannou and Chrysanthi Nikoloudaki and Petros Chronopoulos and Ioannis T Karapanagiotidis and Elena Mente},
url = {https://www.sciencedirect.com/science/article/pii/S0044848625007069
/wp-content/uploads/2025/07/2025-Vasilaki-AQUA-34.pdf},
doi = {10.1016/j.aquaculture.2025.742820},
issn = {0044-8486},
year = {2025},
date = {2025-07-04},
urldate = {2025-07-09},
journal = {Aquaculture},
volume = {609},
pages = {742820},
abstract = {Legumes contain antinutritional factors that restrict their use in fish nutrition, however, biotechnological methods such as solid-state fermentation can improve their nutritional profile, positioning them as a sustainable alternative for aquafeed formulation. This research evaluated the feasibility of replacing soybean meal with Saccharomyces cerevisiae-fermented lupin meal (Lupinus albus) in feed formulations for European sea bass (Dicentrarchus labrax). Four diets were produced; one commercial type diet (FRL0) and three diets with partial (FRL10, FRL12.5) or complete (FRL15) replacement of soybean meal by fermented lupin meal. European sea bass juveniles, with an initial average weight: 18.9 g, were fed the experimental diets for 71 days in triplicate groups. Results indicated no adverse effects on key production indices when soybean meal was replaced partially or totally. Fermented lupin meal linearly improved feed utilization. Apparent digestibility coefficient of protein significantly increased in groups fed 12.5 % and 15 % fermented lupin, as did apparent digestibility coefficient of fat, compared to the FRL0 group. Trypsin activity in pyloric caeca was statistically elevated in the fermented lupin groups compare to FRL0 group. Whole-body composition, amino acid content, and deposition showed no significant differences among groups. Fermented lupin did not suppress the immune system, and a slight immunostimulatory effect was observed at a 12.5 % inclusion level. No histopathological alterations were detected. Overall, the results demonstrated the efficacy of solid-state fermentation process in enhancing lupin meal, highlighting its potential as a sustainable replacement for soybean meal in feed formulations for Mediterranean marine aquaculture species such us D. labrax.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Legumes contain antinutritional factors that restrict their use in fish nutrition, however, biotechnological methods such as solid-state fermentation can improve their nutritional profile, positioning them as a sustainable alternative for aquafeed formulation. This research evaluated the feasibility of replacing soybean meal with Saccharomyces cerevisiae-fermented lupin meal (Lupinus albus) in feed formulations for European sea bass (Dicentrarchus labrax). Four diets were produced; one commercial type diet (FRL0) and three diets with partial (FRL10, FRL12.5) or complete (FRL15) replacement of soybean meal by fermented lupin meal. European sea bass juveniles, with an initial average weight: 18.9 g, were fed the experimental diets for 71 days in triplicate groups. Results indicated no adverse effects on key production indices when soybean meal was replaced partially or totally. Fermented lupin meal linearly improved feed utilization. Apparent digestibility coefficient of protein significantly increased in groups fed 12.5 % and 15 % fermented lupin, as did apparent digestibility coefficient of fat, compared to the FRL0 group. Trypsin activity in pyloric caeca was statistically elevated in the fermented lupin groups compare to FRL0 group. Whole-body composition, amino acid content, and deposition showed no significant differences among groups. Fermented lupin did not suppress the immune system, and a slight immunostimulatory effect was observed at a 12.5 % inclusion level. No histopathological alterations were detected. Overall, the results demonstrated the efficacy of solid-state fermentation process in enhancing lupin meal, highlighting its potential as a sustainable replacement for soybean meal in feed formulations for Mediterranean marine aquaculture species such us D. labrax.
Asimaki, Adamantia; Psofakis, Pier; Gkalogianni, Elli-Zafeiria; Katouni, Aikaterini-Maria; Berillis, Panagiotis; Kormas, Konstantinos A; Rumbos, Christos I; Athanassiou, Christos G; Vasilaki, Antigoni; Fountoulaki, Eleni; Henry, Morgane; Mente, Eleni; Gisbert, Enric; Karapanagiotidis, Ioannis T
In: Aquaculture Nutrition, vol. 2025, no. 1, pp. 8885509, 2025, ISSN: 1353-5773, 1365-2095.
@article{asimaki_effects_2025,
title = {The Effects of Dietary Fishmeal Substitution by Full‐Fat and Defatted textitZophobas morio Larvae Meals on Juvenile Gilthead Seabream (\textit{Sparus aurata}): An Integrative Approach},
author = {Adamantia Asimaki and Pier Psofakis and Elli-Zafeiria Gkalogianni and Aikaterini-Maria Katouni and Panagiotis Berillis and Konstantinos A Kormas and Christos I Rumbos and Christos G Athanassiou and Antigoni Vasilaki and Eleni Fountoulaki and Morgane Henry and Eleni Mente and Enric Gisbert and Ioannis T Karapanagiotidis},
editor = {Lee Seong Wei},
url = {https://onlinelibrary.wiley.com/doi/10.1155/anu/8885509
/wp-content/uploads/2025/06/2025-Asimaki-Aq-Nutr-27.pdf},
doi = {10.1155/anu/8885509},
issn = {1353-5773, 1365-2095},
year = {2025},
date = {2025-06-10},
urldate = {2025-06-10},
journal = {Aquaculture Nutrition},
volume = {2025},
number = {1},
pages = {8885509},
abstract = {This study evaluated the dietary fishmeal substitution by full‐fat (FF) and defatted (DF)
Zophobas morio
meals regarding growth, feed efficiency, proximate and fatty acid compositions, digestive enzymes activities, histology and midgut microbiota in gilthead seabream (
Sparus aurata
). Juveniles initially weighing 3.4 g were distributed to triplicate groups and fed at satiation six isonitrogenous (8.41%) and isocaloric (21 Mj/kg) diets for 100 days. An insect meal‐free diet was the control (CTRL), two diets contained a FF
Z. morio
meal at 49 g/kg (FF‐49) and 97 g/kg (FF‐97), and three diets contained a DF
Z. morio
meal at 58 g/kg (DF‐58), 116 g/kg (DF‐116) and 174 g/kg (DF‐174) at the expense of fishmeal. Neither the form nor the inclusion level of
Z. morio
meals affected the feed intake of fish denoting a similar acceptability to that of fishmeal. Fish survival, growth and feed efficiency were not impaired by all dietary inclusion levels of
Z. morio
meals. Proximate composition of fish was altered but without indicating a clear correlation with the form or inclusion level of
Z. morio
. Increasing inclusions of both forms of
Z. morio
meals tended to decrease 22:6n‐3, 20:5n‐3, 18:3n‐3 and 18:2n‐6 levels in fish tissues. All fish exhibited similar proteolytic enzyme activities, but the increasing inclusions of both insect meal forms led to gradual increases in the lipase and α‐amylase activities indicating a compensatory mechanism for lipid and carbohydrate digestion. The use of
Z. morio
meals led to some mild histomorphological changes in the intestine and liver that were more pronounced in fish fed the FF form at the highest inclusion level. Midgut bacterial communities of the groups were similar and dominated by potentially beneficial members of
Saccharimonadales
and
Rhodobacteraceae
, except FF‐97 fish that had high abundances of
Legionella
‐ and
Pandoraea
‐like bacteria. To conclude,
Z. morio
meal, either FF or DF, is a suitable insect protein for fishmeal substitution towards more sustainable aquafeeds for
S. aurata
.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
This study evaluated the dietary fishmeal substitution by full‐fat (FF) and defatted (DF)
Zophobas morio
meals regarding growth, feed efficiency, proximate and fatty acid compositions, digestive enzymes activities, histology and midgut microbiota in gilthead seabream (
Sparus aurata
). Juveniles initially weighing 3.4 g were distributed to triplicate groups and fed at satiation six isonitrogenous (8.41%) and isocaloric (21 Mj/kg) diets for 100 days. An insect meal‐free diet was the control (CTRL), two diets contained a FF
Z. morio
meal at 49 g/kg (FF‐49) and 97 g/kg (FF‐97), and three diets contained a DF
Z. morio
meal at 58 g/kg (DF‐58), 116 g/kg (DF‐116) and 174 g/kg (DF‐174) at the expense of fishmeal. Neither the form nor the inclusion level of
Z. morio
meals affected the feed intake of fish denoting a similar acceptability to that of fishmeal. Fish survival, growth and feed efficiency were not impaired by all dietary inclusion levels of
Z. morio
meals. Proximate composition of fish was altered but without indicating a clear correlation with the form or inclusion level of
Z. morio
. Increasing inclusions of both forms of
Z. morio
meals tended to decrease 22:6n‐3, 20:5n‐3, 18:3n‐3 and 18:2n‐6 levels in fish tissues. All fish exhibited similar proteolytic enzyme activities, but the increasing inclusions of both insect meal forms led to gradual increases in the lipase and α‐amylase activities indicating a compensatory mechanism for lipid and carbohydrate digestion. The use of
Z. morio
meals led to some mild histomorphological changes in the intestine and liver that were more pronounced in fish fed the FF form at the highest inclusion level. Midgut bacterial communities of the groups were similar and dominated by potentially beneficial members of
Saccharimonadales
and
Rhodobacteraceae
, except FF‐97 fish that had high abundances of
Legionella
‐ and
Pandoraea
‐like bacteria. To conclude,
Z. morio
meal, either FF or DF, is a suitable insect protein for fishmeal substitution towards more sustainable aquafeeds for
S. aurata
.
Zophobas morio
meals regarding growth, feed efficiency, proximate and fatty acid compositions, digestive enzymes activities, histology and midgut microbiota in gilthead seabream (
Sparus aurata
). Juveniles initially weighing 3.4 g were distributed to triplicate groups and fed at satiation six isonitrogenous (8.41%) and isocaloric (21 Mj/kg) diets for 100 days. An insect meal‐free diet was the control (CTRL), two diets contained a FF
Z. morio
meal at 49 g/kg (FF‐49) and 97 g/kg (FF‐97), and three diets contained a DF
Z. morio
meal at 58 g/kg (DF‐58), 116 g/kg (DF‐116) and 174 g/kg (DF‐174) at the expense of fishmeal. Neither the form nor the inclusion level of
Z. morio
meals affected the feed intake of fish denoting a similar acceptability to that of fishmeal. Fish survival, growth and feed efficiency were not impaired by all dietary inclusion levels of
Z. morio
meals. Proximate composition of fish was altered but without indicating a clear correlation with the form or inclusion level of
Z. morio
. Increasing inclusions of both forms of
Z. morio
meals tended to decrease 22:6n‐3, 20:5n‐3, 18:3n‐3 and 18:2n‐6 levels in fish tissues. All fish exhibited similar proteolytic enzyme activities, but the increasing inclusions of both insect meal forms led to gradual increases in the lipase and α‐amylase activities indicating a compensatory mechanism for lipid and carbohydrate digestion. The use of
Z. morio
meals led to some mild histomorphological changes in the intestine and liver that were more pronounced in fish fed the FF form at the highest inclusion level. Midgut bacterial communities of the groups were similar and dominated by potentially beneficial members of
Saccharimonadales
and
Rhodobacteraceae
, except FF‐97 fish that had high abundances of
Legionella
‐ and
Pandoraea
‐like bacteria. To conclude,
Z. morio
meal, either FF or DF, is a suitable insect protein for fishmeal substitution towards more sustainable aquafeeds for
S. aurata
.
2023
Vasilaki, A; Mente, E; Fountoulaki, E; Henry, M; Nikoloudaki, C; Berillis, P; Kousoulaki, K; Nengas, I
Fishmeal, plant protein, and fish oil substitution with single-cell ingredients in organic feeds for European sea bass (Dicentrarchus labrax) Journal Article
In: Frontiers in Physiology, vol. 14, pp. 1199497, 2023, ISSN: 1664-042X.
@article{vasilaki_fishmeal_2023,
title = {Fishmeal, plant protein, and fish oil substitution with single-cell ingredients in organic feeds for European sea bass (Dicentrarchus labrax)},
author = {A Vasilaki and E Mente and E Fountoulaki and M Henry and C Nikoloudaki and P Berillis and K Kousoulaki and I Nengas},
url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/06/2023-Vasilaki-Frontiers-Physio-28.pdf
https://www.frontiersin.org/articles/10.3389/fphys.2023.1199497/full},
doi = {10.3389/fphys.2023.1199497},
issn = {1664-042X},
year = {2023},
date = {2023-06-06},
urldate = {2023-06-12},
journal = {Frontiers in Physiology},
volume = {14},
pages = {1199497},
abstract = {Single-cell ingredients (SCI) are considered promising nutrient sources which are produced using environmentally friendly biotechnological processes. The aim of the current study was to evaluate the replacement of fishmeal, plant protein sources, and fish oil with SCI in organic feeds for European sea bass (
Dicentrarchus labrax
). Bacterial protein, yeast protein, and microalgae were used to replace fishmeal trimmings, soya bean meal, and fish oil from trimmings. Triplicate groups (30 fish per replicate) of European sea bass (14.4 ± 2.4 g) were fed the experimental diets for 71 days. The results showed that the incorporation of SCI at all levels of inclusion significantly enhanced nutrient digestibility. Additionally, growth performance parameters were not affected by SCI inclusion, exhibiting similar or improved values. Moreover, a tendency for improved anterior and posterior gut structure was observed and a significant
increase of lysozyme activity at the two highest inclusion levels of SCI was determined. Overall, the results showed that the inclusion of SCI at 15%
(bacterial: yeast: algae—9.4: 4.7: 1) is possible without compromising any of the parameters evaluated. According to these findings, a higher substitution of fishmeal trimmings, plant protein sources, and fish oil from trimmings with SCI in organic diets for European sea bass (
D. labrax
) can be further evaluated in future studies.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Single-cell ingredients (SCI) are considered promising nutrient sources which are produced using environmentally friendly biotechnological processes. The aim of the current study was to evaluate the replacement of fishmeal, plant protein sources, and fish oil with SCI in organic feeds for European sea bass (
Dicentrarchus labrax
). Bacterial protein, yeast protein, and microalgae were used to replace fishmeal trimmings, soya bean meal, and fish oil from trimmings. Triplicate groups (30 fish per replicate) of European sea bass (14.4 ± 2.4 g) were fed the experimental diets for 71 days. The results showed that the incorporation of SCI at all levels of inclusion significantly enhanced nutrient digestibility. Additionally, growth performance parameters were not affected by SCI inclusion, exhibiting similar or improved values. Moreover, a tendency for improved anterior and posterior gut structure was observed and a significant
increase of lysozyme activity at the two highest inclusion levels of SCI was determined. Overall, the results showed that the inclusion of SCI at 15%
(bacterial: yeast: algae—9.4: 4.7: 1) is possible without compromising any of the parameters evaluated. According to these findings, a higher substitution of fishmeal trimmings, plant protein sources, and fish oil from trimmings with SCI in organic diets for European sea bass (
D. labrax
) can be further evaluated in future studies.
Dicentrarchus labrax
). Bacterial protein, yeast protein, and microalgae were used to replace fishmeal trimmings, soya bean meal, and fish oil from trimmings. Triplicate groups (30 fish per replicate) of European sea bass (14.4 ± 2.4 g) were fed the experimental diets for 71 days. The results showed that the incorporation of SCI at all levels of inclusion significantly enhanced nutrient digestibility. Additionally, growth performance parameters were not affected by SCI inclusion, exhibiting similar or improved values. Moreover, a tendency for improved anterior and posterior gut structure was observed and a significant
increase of lysozyme activity at the two highest inclusion levels of SCI was determined. Overall, the results showed that the inclusion of SCI at 15%
(bacterial: yeast: algae—9.4: 4.7: 1) is possible without compromising any of the parameters evaluated. According to these findings, a higher substitution of fishmeal trimmings, plant protein sources, and fish oil from trimmings with SCI in organic diets for European sea bass (
D. labrax
) can be further evaluated in future studies.
2022
Fountoulaki, E; Vasilaki, A; Nikolopoulou, D; Schrama, J; Kaushik, S J; Prabhu, P Antony Jesu
Faecal waste production, characteristics and recovery in European seabass (Dicentrarchus labrax) is affected by dietary ingredient composition Journal Article
In: Aquaculture, vol. 548, pp. 737582, 2022, ISSN: 00448486.
@article{fountoulaki_faecal_2022b,
title = {Faecal waste production, characteristics and recovery in European seabass (Dicentrarchus labrax) is affected by dietary ingredient composition},
author = {E Fountoulaki and A Vasilaki and D Nikolopoulou and J Schrama and S J Kaushik and P Antony Jesu Prabhu},
url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/03/2022-Fountoulaki-AQUA-preprint-1.pdf
https://linkinghub.elsevier.com/retrieve/pii/S004484862101245X},
doi = {10.1016/j.aquaculture.2021.737582},
issn = {00448486},
year = {2022},
date = {2022-02-01},
urldate = {2022-03-11},
journal = {Aquaculture},
volume = {548},
pages = {737582},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
2021
Rigos, George; Kogiannou, Dimitra; Vasilaki, Antigoni; Kotsiri, Mado
Evaluation of Praziquantel Efficacy against Zeuxapta seriolae Infections in Greater Amberjack, Seriola dumerili Journal Article
In: Applied Sciences, vol. 11, no. 10, pp. 4656, 2021, ISSN: 2076-3417.
@article{rigos_evaluation_2021,
title = {Evaluation of Praziquantel Efficacy against Zeuxapta seriolae Infections in Greater Amberjack, Seriola dumerili},
author = {George Rigos and Dimitra Kogiannou and Antigoni Vasilaki and Mado Kotsiri},
url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/06/2021-Rigos-ApllSciences-41l.pdf
https://www.mdpi.com/2076-3417/11/10/4656},
doi = {10.3390/app11104656},
issn = {2076-3417},
year = {2021},
date = {2021-05-01},
urldate = {2021-06-24},
journal = {Applied Sciences},
volume = {11},
number = {10},
pages = {4656},
abstract = {The aim of this study was to evaluate the efficacy of dietary praziquantel (PZQ), a promising fish anthelminthic, against Zeuxapta seriolae, a lethal ectoparasite of greater amberjack (Seriola dumerili Risso) farmed in the Mediterranean and elsewhere. The trial was carried out in small cages (3 m3) in situ, harbouring fish (125 ± 14 g) naturally infected with Z. seriolae, at 25 ± 1 °C water temperature. Praziquantel-medicated diet (150 mg PZQ/kg fish) was delivered for three days against a control group. Measurement of both adults and oncomiracidia of Z. seriolae in sampled gill arches revealed significant differences between the tested groups (PZQ: 19.3 ± 9.8 vs. Control: 3.8 ± 6.3). Considering an estimated efficacy of 80.4% in the medicated fish, the present study indicates that oral PZQ treatments can confront Z. seriolae infections considerably in farmed greater amberjack and, perhaps, replace the commonly used hydrogen peroxidase baths.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The aim of this study was to evaluate the efficacy of dietary praziquantel (PZQ), a promising fish anthelminthic, against Zeuxapta seriolae, a lethal ectoparasite of greater amberjack (Seriola dumerili Risso) farmed in the Mediterranean and elsewhere. The trial was carried out in small cages (3 m3) in situ, harbouring fish (125 ± 14 g) naturally infected with Z. seriolae, at 25 ± 1 °C water temperature. Praziquantel-medicated diet (150 mg PZQ/kg fish) was delivered for three days against a control group. Measurement of both adults and oncomiracidia of Z. seriolae in sampled gill arches revealed significant differences between the tested groups (PZQ: 19.3 ± 9.8 vs. Control: 3.8 ± 6.3). Considering an estimated efficacy of 80.4% in the medicated fish, the present study indicates that oral PZQ treatments can confront Z. seriolae infections considerably in farmed greater amberjack and, perhaps, replace the commonly used hydrogen peroxidase baths.
2020
Henry, Morgane A; Fountoulaki, Eleni; Vasilaki, Antigoni; Rigos, George; Kokou, Fotini; Karalazos, Vasileios
In: Aquaculture, vol. 528, pp. 735479, 2020, ISSN: 0044-8486.
@article{henry_dietary_2020,
title = {Dietary micronutrient supplementation in low fishmeal based diets for optimum growth and immune status of European sea bass (Dicentrarchus labrax) juveniles},
author = {Morgane A Henry and Eleni Fountoulaki and Antigoni Vasilaki and George Rigos and Fotini Kokou and Vasileios Karalazos},
url = {http://www.sciencedirect.com/science/article/pii/S0044848620312412},
doi = {https://doi.org/10.1016/j.aquaculture.2020.735479},
issn = {0044-8486},
year = {2020},
date = {2020-01-01},
journal = {Aquaculture},
volume = {528},
pages = {735479},
abstract = {The present study aimed to evaluate the optimal dietary micronutrient supplementation of plant-based diets for juvenile European sea bass. A 90-day feeding trial was performed, where fish were fed a high fishmeal (FM) based diet (62% DM; positive control) or low FM diets (12.5% FM and krill meal), supplemented with increasing levels of a micronutrient mixture (Nutrient Package, NP) consisting of vitamins, minerals and other micronutrients. The supplementation levels ranged from 0% (no supplementation, negative control) to 400%, where 100% supplementation level corresponded to the requirement levels recommended for other fish species. Fish growth and feed performance (SGR, DGI, FCR, PER) were significantly impaired at levels NP0 and NP25 compared to the FM control. Body Fe and Zn concentrations were positively affected by the graded levels of the NP, while Mn and Se concentrations remained stable. Growth parameters were particularly correlated to body Fe and Zn, while immune parameters were mainly correlated to body Zn. Significant immunosuppression was evidenced (concerning the respiratory burst activity) when low FM diets were not supplemented with NP. High inclusion levels of the NP showed a negative impact on lysozyme activity. Histological examinations did not reveal any significant effects on gills, gut and liver morphology. At identical NP supplementation level (50%), fish fed low and high FM diets showed similar growth and innate immunity suggesting that long-term feeding with plant-based diets do not significantly affect the fish when adequate minerals and vitamins supplementation is provided. Quadratic regression analysis showed that the inclusion of the NP between 260 and 300% improves growth and immune parameters of fish fed low-FM diets as well as Zn body incorporation. Overall, the present study suggested the positive impact of NP supplementation of PM-based diets on both fish growth and health but future NP could include less Mn, more Fe, closer to levels obtained in fish fed FM (245 mg·kg−1), and Zn at 122 mg·kg−1.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The present study aimed to evaluate the optimal dietary micronutrient supplementation of plant-based diets for juvenile European sea bass. A 90-day feeding trial was performed, where fish were fed a high fishmeal (FM) based diet (62% DM; positive control) or low FM diets (12.5% FM and krill meal), supplemented with increasing levels of a micronutrient mixture (Nutrient Package, NP) consisting of vitamins, minerals and other micronutrients. The supplementation levels ranged from 0% (no supplementation, negative control) to 400%, where 100% supplementation level corresponded to the requirement levels recommended for other fish species. Fish growth and feed performance (SGR, DGI, FCR, PER) were significantly impaired at levels NP0 and NP25 compared to the FM control. Body Fe and Zn concentrations were positively affected by the graded levels of the NP, while Mn and Se concentrations remained stable. Growth parameters were particularly correlated to body Fe and Zn, while immune parameters were mainly correlated to body Zn. Significant immunosuppression was evidenced (concerning the respiratory burst activity) when low FM diets were not supplemented with NP. High inclusion levels of the NP showed a negative impact on lysozyme activity. Histological examinations did not reveal any significant effects on gills, gut and liver morphology. At identical NP supplementation level (50%), fish fed low and high FM diets showed similar growth and innate immunity suggesting that long-term feeding with plant-based diets do not significantly affect the fish when adequate minerals and vitamins supplementation is provided. Quadratic regression analysis showed that the inclusion of the NP between 260 and 300% improves growth and immune parameters of fish fed low-FM diets as well as Zn body incorporation. Overall, the present study suggested the positive impact of NP supplementation of PM-based diets on both fish growth and health but future NP could include less Mn, more Fe, closer to levels obtained in fish fed FM (245 mg·kg−1), and Zn at 122 mg·kg−1.
2019
Kokou, Fotini; Henry, Morgane; Nikoloudaki, Chrisa; Kounna, Christiana; Vasilaki, Antigoni; Fountoulaki, Eleni
Optimum protein-to-lipid ratio requirement of the juvenile shi drum (Umbrina cirrosa) as estimated by nutritional and histological parameters Journal Article
In: Aquaculture Nutrition, vol. 25, no. 2, pp. 444–455, 2019, (_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/anu.12870).
@article{kokou_optimum_2019,
title = {Optimum protein-to-lipid ratio requirement of the juvenile shi drum (Umbrina cirrosa) as estimated by nutritional and histological parameters},
author = {Fotini Kokou and Morgane Henry and Chrisa Nikoloudaki and Christiana Kounna and Antigoni Vasilaki and Eleni Fountoulaki},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/anu.12870},
doi = {10.1111/anu.12870},
year = {2019},
date = {2019-01-01},
journal = {Aquaculture Nutrition},
volume = {25},
number = {2},
pages = {444--455},
abstract = {Abstract The shi drum is an emerging Mediterranean aquaculture species that has gained increasing interest in recent years. However, the nutrient requirements of this species remain to be determined to optimize its culture. The present study evaluated the effects of dietary protein (470g/kg or 520g/kg) to lipid ratios (100g/kg, 150 or 200g/kg) on the growth performance, nutrient utilization and tissue morphology of the shi drum (7 g average initial weight). Dietary protein levels positively correlated with weight gain, showing high protein demands (textgreater470g/kg) for this species. Nonetheless, dietary lipid levels negatively affected growth rate, feed intake and efficiency, and protein utilization, suggesting that this macronutrient cannot be efficiently utilized in high concentrations (textgreater100g/kg). Histological evaluation revealed fat accumulation within the hepatocytes and the enterocytes with increasing dietary lipid levels, starting from 150g/kg lipid inclusion. Overall, our results confirm the high dietary protein requirements of the shi drum (textgreater470g/kg for fish of 7 g initial weight) and suggest that lipid levels should not exceed 100g/kg of inclusion in the diets as this species has a low tolerance to this macronutrient.},
note = {_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/anu.12870},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Abstract The shi drum is an emerging Mediterranean aquaculture species that has gained increasing interest in recent years. However, the nutrient requirements of this species remain to be determined to optimize its culture. The present study evaluated the effects of dietary protein (470g/kg or 520g/kg) to lipid ratios (100g/kg, 150 or 200g/kg) on the growth performance, nutrient utilization and tissue morphology of the shi drum (7 g average initial weight). Dietary protein levels positively correlated with weight gain, showing high protein demands (textgreater470g/kg) for this species. Nonetheless, dietary lipid levels negatively affected growth rate, feed intake and efficiency, and protein utilization, suggesting that this macronutrient cannot be efficiently utilized in high concentrations (textgreater100g/kg). Histological evaluation revealed fat accumulation within the hepatocytes and the enterocytes with increasing dietary lipid levels, starting from 150g/kg lipid inclusion. Overall, our results confirm the high dietary protein requirements of the shi drum (textgreater470g/kg for fish of 7 g initial weight) and suggest that lipid levels should not exceed 100g/kg of inclusion in the diets as this species has a low tolerance to this macronutrient.
2016
Grigorakis, K; Alexi, N; Vasilaki, A; Giogios, I; Fountoulaki, E
Chemical quality and sensory profile of the mediterranean farmed fish shi drum (Umbrina cirrosa) as affected by its dietary protein/fat levels Journal Article
In: Italian Journal of Animal Science, vol. 15, no. 4, pp. 681–688, 2016, ISSN: 15944077, (Publisher: Taylor and Francis Ltd.).
@article{grigorakis_chemical_2016,
title = {Chemical quality and sensory profile of the mediterranean farmed fish shi drum (Umbrina cirrosa) as affected by its dietary protein/fat levels},
author = {K Grigorakis and N Alexi and A Vasilaki and I Giogios and E Fountoulaki},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84996565541&doi=10.1080%2f1828051X.2016.1222890&partnerID=40&md5=991fee64ac8d2146e822844d0c584c62},
doi = {10.1080/1828051X.2016.1222890},
issn = {15944077},
year = {2016},
date = {2016-01-01},
journal = {Italian Journal of Animal Science},
volume = {15},
number = {4},
pages = {681--688},
abstract = {Two groups of identically-reared shi drum,having received different diets (Group A: 45% protein and 16% fat and Group B: 48% protein and 12% fat),were compared for their yields and their chemical and sensory quality. They exhibited similar dressing and filleting yields,fat deposit and fillet composition. Differences were observed in the fillet fatty acids,with group B exhibiting higher 16:1ω-7,16:1ω-9,16:0,18:0,and total saturate contents. Their fillet volatile compounds also differed (group A,in particular,contained higher levels of carbonyl-compounds). A triangle test revealed that the two shi drum groups were perceived as sensory different. A Check-All-That-Apply (CATA) test showed that group A (high dietary lipids) was perceived as having a significantly higher ‘fish oil’ aroma,hardness and elasticity,while group B was characterised mainly by higher ‘sweet taste’,higher ‘hay’ and ‘fresh seaweed’ aroma and ‘crab/prawn’ flavour. © 2016 The Author(s).},
note = {Publisher: Taylor and Francis Ltd.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Two groups of identically-reared shi drum,having received different diets (Group A: 45% protein and 16% fat and Group B: 48% protein and 12% fat),were compared for their yields and their chemical and sensory quality. They exhibited similar dressing and filleting yields,fat deposit and fillet composition. Differences were observed in the fillet fatty acids,with group B exhibiting higher 16:1ω-7,16:1ω-9,16:0,18:0,and total saturate contents. Their fillet volatile compounds also differed (group A,in particular,contained higher levels of carbonyl-compounds). A triangle test revealed that the two shi drum groups were perceived as sensory different. A Check-All-That-Apply (CATA) test showed that group A (high dietary lipids) was perceived as having a significantly higher ‘fish oil’ aroma,hardness and elasticity,while group B was characterised mainly by higher ‘sweet taste’,higher ‘hay’ and ‘fresh seaweed’ aroma and ‘crab/prawn’ flavour. © 2016 The Author(s).
2011
Grigorakis, Kriton; Fountoulaki, Eleni; Vasilaki, Antigoni; Mittakos, Ioannis; Nathanailides, Cosmas
Lipid quality and filleting yield of reared meagre (Argyrosomus regius): Lipid quality & filleting yield of reared meagre Journal Article
In: International Journal of Food Science & Technology, vol. 46, no. 4, pp. 711–716, 2011, ISSN: 09505423.
@article{grigorakis_lipid_2011,
title = {Lipid quality and filleting yield of reared meagre (Argyrosomus regius): Lipid quality & filleting yield of reared meagre},
author = {Kriton Grigorakis and Eleni Fountoulaki and Antigoni Vasilaki and Ioannis Mittakos and Cosmas Nathanailides},
url = {http://doi.wiley.com/10.1111/j.1365-2621.2010.02537.x},
doi = {10.1111/j.1365-2621.2010.02537.x},
issn = {09505423},
year = {2011},
date = {2011-04-01},
urldate = {2020-08-10},
journal = {International Journal of Food Science & Technology},
volume = {46},
number = {4},
pages = {711--716},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
2010
Grigorakis, K; Giogios, I; Vasilaki, A; Nengas, I
Effect of the fish oil, oxidation status and of heat treatment temperature on the volatile compounds of the produced fish feeds Journal Article
In: Animal Feed Science and Technology, vol. 158, no. 1-2, pp. 73–84, 2010, ISSN: 03778401.
@article{grigorakis_effect_2010,
title = {Effect of the fish oil, oxidation status and of heat treatment temperature on the volatile compounds of the produced fish feeds},
author = {K Grigorakis and I Giogios and A Vasilaki and I Nengas},
url = {https://linkinghub.elsevier.com/retrieve/pii/S0377840110000970},
doi = {10.1016/j.anifeedsci.2010.03.012},
issn = {03778401},
year = {2010},
date = {2010-06-01},
urldate = {2020-08-10},
journal = {Animal Feed Science and Technology},
volume = {158},
number = {1-2},
pages = {73--84},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Fountoulaki, E; Henry, M; Rigos, G; Vasilaki, A; Mente, E; Sweetman, J; Nengas, I
Evaluation of zinc supplementation in European sea bass (Dicentrarchus labrax) juvenile diets Journal Article
In: Aquaculture Research, vol. 41, pp. e208 – e216, 2010.
@article{fountoulaki_evaluation_2010,
title = {Evaluation of zinc supplementation in European sea bass (Dicentrarchus labrax) juvenile diets},
author = {E Fountoulaki and M Henry and G Rigos and A Vasilaki and E Mente and J Sweetman and I Nengas},
url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77955955157&partnerID=40&md5=998c56a51dbd36e139c6f19395287c12},
doi = {10.1111/j.1365-2109.2010.02503.x},
year = {2010},
date = {2010-01-01},
journal = {Aquaculture Research},
volume = {41},
pages = {e208 -- e216},
abstract = {This study aimed to investigate the effect of increased zinc dietary levels on the growth performance, feed utilization, immune status and induced wound healing in European sea bass (Dicentrarchus labrax). Fish weighing 10 g were fed five diets containing organic zinc at 30, 70, 110 and 150 mg kg -1 diet and one inorganic zinc source (zinc oxide) at 150 mg kg -1 diet for a period of 12 weeks. No significant (P>0.05) differences were found in the growth performance parameters (final weight, specific growth rate), immunological indices tested (respiratory burst activity in whole blood) and the wound-healing process. Zinc accumulation in the skin, vertebrae and liver increased significantly (P<0.01) with increased dietary zinc levels but not in muscle. The highest zinc concentrations were obtained in skin tissue, followed by vertebrae, liver and muscle tissue. Using the concentration of zinc in skin as a response criterion, broken-line analysis showed that the supplementation of 148 mg organic Zn kg -1 diet seemed to be the optimum dietary zinc supplementation level for sea bass juveniles. © 2010 The Authors. Journal Compilation © 2010 Blackwell Publishing Ltd.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
This study aimed to investigate the effect of increased zinc dietary levels on the growth performance, feed utilization, immune status and induced wound healing in European sea bass (Dicentrarchus labrax). Fish weighing 10 g were fed five diets containing organic zinc at 30, 70, 110 and 150 mg kg -1 diet and one inorganic zinc source (zinc oxide) at 150 mg kg -1 diet for a period of 12 weeks. No significant (P>0.05) differences were found in the growth performance parameters (final weight, specific growth rate), immunological indices tested (respiratory burst activity in whole blood) and the wound-healing process. Zinc accumulation in the skin, vertebrae and liver increased significantly (P<0.01) with increased dietary zinc levels but not in muscle. The highest zinc concentrations were obtained in skin tissue, followed by vertebrae, liver and muscle tissue. Using the concentration of zinc in skin as a response criterion, broken-line analysis showed that the supplementation of 148 mg organic Zn kg -1 diet seemed to be the optimum dietary zinc supplementation level for sea bass juveniles. © 2010 The Authors. Journal Compilation © 2010 Blackwell Publishing Ltd.
2009
Fountoulaki, E; Vasilaki, A; Hurtado, R; Grigorakis, K; Karacostas, I; Nengas, I; Rigos, G; Kotzamanis, Y; Venou, B; Alexis, M N
In: Aquaculture, vol. 289, no. 3-4, pp. 317–326, 2009, ISSN: 00448486.
@article{fountoulaki_fish_2009,
title = {Fish oil substitution by vegetable oils in commercial diets for gilthead sea bream (Sparus aurata L.); effects on growth performance, flesh quality and fillet fatty acid profile. Recovery of fatty acid profiles by a fish oil finishing diet under fluctuating water temperatures},
author = {E Fountoulaki and A Vasilaki and R Hurtado and K Grigorakis and I Karacostas and I Nengas and G Rigos and Y Kotzamanis and B Venou and M N Alexis},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-61649088364&doi=10.1016%2fj.aquaculture.2009.01.023&partnerID=40&md5=e3b4d18877877d60dcd12caa1fda3f45},
doi = {10.1016/j.aquaculture.2009.01.023},
issn = {00448486},
year = {2009},
date = {2009-01-01},
journal = {Aquaculture},
volume = {289},
number = {3-4},
pages = {317--326},
abstract = {The effects of long term feeding (6 months) of commercial diets with low fish meal content and high levels of vegetable oils (69% fish oil substitution level) were determined in gilthead sea bream (110 g). A control diet containing South American fish oil (FO) was evaluated against feeds with either soybean oil (SO), palm oil (PO) or rapeseed oil (RO). Afterwards, all fish were fed a fish oil finishing diet to determine the progressive recovery of the fillet fatty acid profiles. The results showed that growth and feed utilization in gilthead sea bream are not affected by fish oil substitution with soybean and rapeseed oil, contrary to palm oil inclusion. Flesh and liver docosahexaenoic acid (DHA) and arachidonic acid (ArA) contents were reduced to a lower degree than their reduction in the diet, whereas eicosapentaenoic (EPA) reduction was more pronounced in both tissues. Sensory analysis revealed no difference in the organoleptic characteristics of the dietary groups. However, low acceptance scores were calculated for all treated groups. No histological alterations were seen in gut tissue but liver of the PO group showed intense lipid accumulation. Re-feeding with a fish oil finishing diet for 120 days was not adequate for restoration of DHA, ArA and EPA. Linoleic (LA) and oleic acid (OA) were retained even after 120 days re-feeding with the fish oil diet. © 2009 Elsevier B.V. All rights reserved.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The effects of long term feeding (6 months) of commercial diets with low fish meal content and high levels of vegetable oils (69% fish oil substitution level) were determined in gilthead sea bream (110 g). A control diet containing South American fish oil (FO) was evaluated against feeds with either soybean oil (SO), palm oil (PO) or rapeseed oil (RO). Afterwards, all fish were fed a fish oil finishing diet to determine the progressive recovery of the fillet fatty acid profiles. The results showed that growth and feed utilization in gilthead sea bream are not affected by fish oil substitution with soybean and rapeseed oil, contrary to palm oil inclusion. Flesh and liver docosahexaenoic acid (DHA) and arachidonic acid (ArA) contents were reduced to a lower degree than their reduction in the diet, whereas eicosapentaenoic (EPA) reduction was more pronounced in both tissues. Sensory analysis revealed no difference in the organoleptic characteristics of the dietary groups. However, low acceptance scores were calculated for all treated groups. No histological alterations were seen in gut tissue but liver of the PO group showed intense lipid accumulation. Re-feeding with a fish oil finishing diet for 120 days was not adequate for restoration of DHA, ArA and EPA. Linoleic (LA) and oleic acid (OA) were retained even after 120 days re-feeding with the fish oil diet. © 2009 Elsevier B.V. All rights reserved.