2022 |
de Mello, Paulo H; Divanach, Pascal; Papadakis, Ioannis E Aquaculture Research, 53 (9), pp. 3416–3429, 2022, ISSN: 1355-557X, 1365-2109. @article{de_mello_temperature_2022, title = {Temperature influences growth, digestive system ontogeny and lipids deposition in the liver in gilthead seabream ( textitSparus aurata ) larvae and juveniles}, author = {Paulo H de Mello and Pascal Divanach and Ioannis E Papadakis}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/07/2022-de-Mello-ARE-pre-print-44.pdf https://onlinelibrary.wiley.com/doi/10.1111/are.15849}, doi = {10.1111/are.15849}, issn = {1355-557X, 1365-2109}, year = {2022}, date = {2022-06-01}, urldate = {2022-07-29}, journal = {Aquaculture Research}, volume = {53}, number = {9}, pages = {3416--3429}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2021 |
Doxa, Chrysa K; Sterioti, Aspasia; Divanach, Pascal; Kentouri, Maroudio Effect of temperature on embryonic development of the marine gastropod Charonia seguenzae (Aradas & Benoit, 1870) Journal Article Journal of Thermal Biology, 100 , pp. 103044, 2021, ISSN: 03064565, (AQUA). @article{doxa_effect_2021, title = {Effect of temperature on embryonic development of the marine gastropod Charonia seguenzae (Aradas & Benoit, 1870)}, author = {Chrysa K Doxa and Aspasia Sterioti and Pascal Divanach and Maroudio Kentouri}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0306456521002126}, doi = {10.1016/j.jtherbio.2021.103044}, issn = {03064565}, year = {2021}, date = {2021-01-01}, urldate = {2022-01-17}, journal = {Journal of Thermal Biology}, volume = {100}, pages = {103044}, note = {AQUA}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2019 |
Doxa, Chrysa K; Sterioti, Aspasia; Divanach, Pascal; Kentouri, Maroudio Reproductive behavior of the marine gastropod Charonia seguenzae (Aradas & Benoit, 1870) in captivity Journal Article Mediterranean Marine Science, 2019, ISSN: 1791-6763, 1108-393X. @article{doxa_reproductive_2019, title = {Reproductive behavior of the marine gastropod Charonia seguenzae (Aradas & Benoit, 1870) in captivity}, author = {Chrysa K Doxa and Aspasia Sterioti and Pascal Divanach and Maroudio Kentouri}, url = {https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/14917}, doi = {10.12681/mms.14917}, issn = {1791-6763, 1108-393X}, year = {2019}, date = {2019-01-01}, urldate = {2022-02-22}, journal = {Mediterranean Marine Science}, abstract = {The reproductive behavior of the gastropod Charonia seguenzae (Aradas & Benoit, 1870) was studied through the description of 19 copulation and 21 egg laying events of 134 wild individuals. Findings in the present study regarding a part of the reproduction temperature range (20 to 23oC) and the demonstration of maternal care provided important information on their biology, behavior and ecology. Furthermore, observed polyandry by the females and collaborative care of embryonic sacks, were two aspects of the species reproductive biology that pose new questions both at ecological and evolutionary level.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The reproductive behavior of the gastropod Charonia seguenzae (Aradas & Benoit, 1870) was studied through the description of 19 copulation and 21 egg laying events of 134 wild individuals. Findings in the present study regarding a part of the reproduction temperature range (20 to 23oC) and the demonstration of maternal care provided important information on their biology, behavior and ecology. Furthermore, observed polyandry by the females and collaborative care of embryonic sacks, were two aspects of the species reproductive biology that pose new questions both at ecological and evolutionary level. |
2018 |
Papadakis, I E; Kentouri, M; Divanach, P; Mylonas, C C Ontogeny of the eye of meagre (Argyrosomus regius) from hatching to juvenile and implications to commercial larval rearing Journal Article Aquaculture, 484 , pp. 32–43, 2018, ISSN: 00448486, (Publisher: Elsevier B.V.). @article{papadakis_ontogeny_2018, title = {Ontogeny of the eye of meagre (Argyrosomus regius) from hatching to juvenile and implications to commercial larval rearing}, author = {I E Papadakis and M Kentouri and P Divanach and C C Mylonas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85032915502&doi=10.1016%2fj.aquaculture.2017.10.038&partnerID=40&md5=6d5582db60caf9774e0ef281ba47a57f}, doi = {10.1016/j.aquaculture.2017.10.038}, issn = {00448486}, year = {2018}, date = {2018-01-01}, journal = {Aquaculture}, volume = {484}, pages = {32--43}, abstract = {The histological development of the visual system of meagre (Argyrosomus regius) was studied from hatching to juvenile stage (44 days post hatching, dph), under aquaculture rearing conditions. The cones, horizontal, bipolar, amacrine and ganglia cells appeared already at 3 dph at a total body length (TL) of 3.11 ± 0.21 mm, at the stage of mouth opening. Rods appeared at 6 dph (TL 4.16 ± 0.73 mm) and increased their density continually during the monitoring period. On the contrary at the same period, the evolution of the number of cones, horizontal, bipolar, amacrine and ganglia cells in the retina were reduced. While photopic sensitivity increased over time, meagre may be considered as a species with well-developed scotopic sensitivity as well, already by 17 dph (TL 8.14 ± 1.64 mm). This observation suggests that instead of the commonly used 24-h continuous high light intensities used in larval rearing protocols of meagre, lower light intensities should be employed, especially during the night hours. Histological visual acuity increased with larval development and appeared to stabilize by the early juvenile stage. Based on the estimated histological visual acuity and the theoretical distance at which rotifers and Artemia may be identified by meagre larvae, minimum prey densities should be 0.5 rotifers and 0.009 Artemia ml− 1 during their first use as feed items. The study demonstrated that the majority of ontogenetic events that are related with retinal differentiation and the function of the eye occurred very early during larval development in meagre. The results provide important information for the biology of the species, as well as for the optimization of larval rearing conditions regarding light management and prey item density under aquaculture conditions. © 2017 Elsevier B.V.}, note = {Publisher: Elsevier B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The histological development of the visual system of meagre (Argyrosomus regius) was studied from hatching to juvenile stage (44 days post hatching, dph), under aquaculture rearing conditions. The cones, horizontal, bipolar, amacrine and ganglia cells appeared already at 3 dph at a total body length (TL) of 3.11 ± 0.21 mm, at the stage of mouth opening. Rods appeared at 6 dph (TL 4.16 ± 0.73 mm) and increased their density continually during the monitoring period. On the contrary at the same period, the evolution of the number of cones, horizontal, bipolar, amacrine and ganglia cells in the retina were reduced. While photopic sensitivity increased over time, meagre may be considered as a species with well-developed scotopic sensitivity as well, already by 17 dph (TL 8.14 ± 1.64 mm). This observation suggests that instead of the commonly used 24-h continuous high light intensities used in larval rearing protocols of meagre, lower light intensities should be employed, especially during the night hours. Histological visual acuity increased with larval development and appeared to stabilize by the early juvenile stage. Based on the estimated histological visual acuity and the theoretical distance at which rotifers and Artemia may be identified by meagre larvae, minimum prey densities should be 0.5 rotifers and 0.009 Artemia ml− 1 during their first use as feed items. The study demonstrated that the majority of ontogenetic events that are related with retinal differentiation and the function of the eye occurred very early during larval development in meagre. The results provide important information for the biology of the species, as well as for the optimization of larval rearing conditions regarding light management and prey item density under aquaculture conditions. © 2017 Elsevier B.V. |
2014 |
Loizides, M; Georgakopoulou, E; Christou, M; Iliopoulou, M; Papadakis, I; Katharios, P; Divanach, P; Koumoundouros, G Thermally-induced phenotypic plasticity in gilthead seabream Sparus aurata L. (Perciformes, Sparidae) Journal Article Aquaculture, 432 , pp. 383–388, 2014, ISSN: 00448486, (Publisher: Elsevier). @article{loizides_thermally-induced_2014, title = {Thermally-induced phenotypic plasticity in gilthead seabream Sparus aurata L. (Perciformes, Sparidae)}, author = {M Loizides and E Georgakopoulou and M Christou and M Iliopoulou and I Papadakis and P Katharios and P Divanach and G Koumoundouros}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84907594566&doi=10.1016%2fj.aquaculture.2014.06.002&partnerID=40&md5=2d0fb32cff829342c38f272f14f01d7d}, doi = {10.1016/j.aquaculture.2014.06.002}, issn = {00448486}, year = {2014}, date = {2014-01-01}, journal = {Aquaculture}, volume = {432}, pages = {383--388}, abstract = {Finfish present a great ability to modify their phenotype in response to environmental conditions especially in the early ontogenetic period. Such plastic phenotypic responses have a great importance, from both the ecological and aquaculture point of view. The present study examined the effect of water temperature on the ontogeny and juvenile phenotype of Sparus aurata L. Six combinations of three temperatures (16, 19, and 22. °C) were applied during different phases of ontogeny up to the juvenile stage (ca 33-37. mm standard length, SL). Elevated temperature resulted in a relative acceleration of differentiation versus growth, which morphologically was evident as a significantly smaller SL (ca 11% decrease) at different ontogenetic events (development of fins, squamation and body shape). Moreover, geometric morphometric analysis clearly showed a significant effect of water temperature during early ontogeny on the juvenile body-shape. Thermally-induced variation of juvenile body shape was mainly expressed across the first canonical axis (56.4% explained variance), which discriminated the groups of highest temperatures from the rest. Water temperature mainly affected the position of the bases of supraoccipital and of basioccipital bones, of the upper jaw, as well as of the dorsal, anal and pelvic fins. The results are discussed with respect to the potential use of thermal manipulations during the hatchery phase to control the phenotype of S. aurata at the end of on-growing phase, i.e. at commercial size. © 2014 Elsevier B.V.}, note = {Publisher: Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {article} } Finfish present a great ability to modify their phenotype in response to environmental conditions especially in the early ontogenetic period. Such plastic phenotypic responses have a great importance, from both the ecological and aquaculture point of view. The present study examined the effect of water temperature on the ontogeny and juvenile phenotype of Sparus aurata L. Six combinations of three temperatures (16, 19, and 22. °C) were applied during different phases of ontogeny up to the juvenile stage (ca 33-37. mm standard length, SL). Elevated temperature resulted in a relative acceleration of differentiation versus growth, which morphologically was evident as a significantly smaller SL (ca 11% decrease) at different ontogenetic events (development of fins, squamation and body shape). Moreover, geometric morphometric analysis clearly showed a significant effect of water temperature during early ontogeny on the juvenile body-shape. Thermally-induced variation of juvenile body shape was mainly expressed across the first canonical axis (56.4% explained variance), which discriminated the groups of highest temperatures from the rest. Water temperature mainly affected the position of the bases of supraoccipital and of basioccipital bones, of the upper jaw, as well as of the dorsal, anal and pelvic fins. The results are discussed with respect to the potential use of thermal manipulations during the hatchery phase to control the phenotype of S. aurata at the end of on-growing phase, i.e. at commercial size. © 2014 Elsevier B.V. |
Papandroulakis, N; Lika, K; Kristiansen, T S; Oppedal, F; Divanach, P; Pavlidis, M Behaviour of European sea bass, Dicentrarchus labrax L., in cages - impact of early life rearing conditions and management Journal Article Aquaculture Research, 45 (9), pp. 1545–1558, 2014, ISSN: 1355557X, (Publisher: Blackwell Publishing Ltd). @article{papandroulakis_behaviour_2014, title = {Behaviour of European sea bass, Dicentrarchus labrax L., in cages - impact of early life rearing conditions and management}, author = {N Papandroulakis and K Lika and T S Kristiansen and F Oppedal and P Divanach and M Pavlidis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84904957506&doi=10.1111%2fare.12103&partnerID=40&md5=2b75258c38f880692350144e12b4c676}, doi = {10.1111/are.12103}, issn = {1355557X}, year = {2014}, date = {2014-01-01}, journal = {Aquaculture Research}, volume = {45}, number = {9}, pages = {1545--1558}, abstract = {European sea bass, Dicentrarchus labrax L., groups reared under different conditions during larval stages (mesocosm and intensive rearing) were monitored during on-growing in sea cages until marketable size (350-400 g). Four caged groups were followed for a period of 17 months each at a pilot scale farm, where vertical distribution behaviour was monitored. This was performed while fish were under calm conditions, during feeding and when stress events occurred. Also during two specific periods: (i) spawning and (ii) high water temperature. Clear differences in the behavioural pattern of swimming depth, displacement and used space between the groups were observed. The individuals from the mesocosm rearing were more sensitive to human presence, showing stronger reactions (speed of displacement and vertical distribution). Most pronounced differences were observed during the 'extreme' warm period and the reproductive season. Within the seasons, European sea bass responded during feeding and stress showing a tendency to move deeper compared to calm conditions. The increased displacements were longer during feeding and stress. © 2012 John Wiley & Sons Ltd.}, note = {Publisher: Blackwell Publishing Ltd}, keywords = {}, pubstate = {published}, tppubtype = {article} } European sea bass, Dicentrarchus labrax L., groups reared under different conditions during larval stages (mesocosm and intensive rearing) were monitored during on-growing in sea cages until marketable size (350-400 g). Four caged groups were followed for a period of 17 months each at a pilot scale farm, where vertical distribution behaviour was monitored. This was performed while fish were under calm conditions, during feeding and when stress events occurred. Also during two specific periods: (i) spawning and (ii) high water temperature. Clear differences in the behavioural pattern of swimming depth, displacement and used space between the groups were observed. The individuals from the mesocosm rearing were more sensitive to human presence, showing stronger reactions (speed of displacement and vertical distribution). Most pronounced differences were observed during the 'extreme' warm period and the reproductive season. Within the seasons, European sea bass responded during feeding and stress showing a tendency to move deeper compared to calm conditions. The increased displacements were longer during feeding and stress. © 2012 John Wiley & Sons Ltd. |
2013 |
NE., Ioannidis; A., Papazi; G., Tsoukali; T., Tsiavos; P., Katharios; P., Divanach; Kotzabasis, K The physiology of Chlorella minutissima cultures in a modern low cost photobioreactor designed for high productivity. Inproceedings HBS, (Ed.): Thessaloniki, Greece, 2013. @inproceedings{ioannidis_physiology_2013, title = {The physiology of Chlorella minutissima cultures in a modern low cost photobioreactor designed for high productivity.}, author = {Ioannidis NE. and Papazi A. and Tsoukali G. and Tsiavos T. and Katharios P. and Divanach P. and K Kotzabasis}, editor = {HBS}, year = {2013}, date = {2013-10-01}, address = {Thessaloniki, Greece}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
V., Kostopoulou; A., Tsopelakos; E., Zogopoulou; H., Miliou; P., Divanach; Katharios, P The effect of different phytoplankton species and commercial enrichment products on the fatty acid profile, enzyme activity and overall condition of the rotifer Brachionus plicatilis. Inproceedings of Ghent, University (Ed.): Larve 2013, pp. 219–222, Ghent, Belgium, 2013. @inproceedings{kostopoulou_effect_2013-1, title = {The effect of different phytoplankton species and commercial enrichment products on the fatty acid profile, enzyme activity and overall condition of the rotifer Brachionus plicatilis.}, author = {Kostopoulou V. and Tsopelakos A. and Zogopoulou E. and Miliou H. and Divanach P. and P Katharios}, editor = {University of Ghent}, year = {2013}, date = {2013-09-01}, booktitle = {Larve 2013}, pages = {219--222}, address = {Ghent, Belgium}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Doxa, Chrisa K; Divanach, Pascal; Kentouri, Maroudio Consumption rates and digestibility of four food items by the marine gastropod Charonia seguenzae (Aradas & Benoit, 1870) Journal Article Journal of Experimental Marine Biology and Ecology, 446 , pp. 10–16, 2013, ISSN: 00220981. @article{doxa_consumption_2013, title = {Consumption rates and digestibility of four food items by the marine gastropod Charonia seguenzae (Aradas & Benoit, 1870)}, author = {Chrisa K Doxa and Pascal Divanach and Maroudio Kentouri}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0022098113001731}, doi = {10.1016/j.jembe.2013.04.019}, issn = {00220981}, year = {2013}, date = {2013-08-01}, urldate = {2020-08-17}, journal = {Journal of Experimental Marine Biology and Ecology}, volume = {446}, pages = {10--16}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Doxa, Chrisa K; Kentouri, Maroudio; Divanach, Pascal Feeding of Charonia seguenzae (Aradas & Benoit, 1870) on natural prey and alternative foods Journal Article Journal of Molluscan Studies, 79 (1), pp. 76–78, 2013, ISSN: 0260-1230, 1464-3766. @article{doxa_feeding_2013, title = {Feeding of Charonia seguenzae (Aradas & Benoit, 1870) on natural prey and alternative foods}, author = {Chrisa K Doxa and Maroudio Kentouri and Pascal Divanach}, url = {https://academic.oup.com/mollus/article-lookup/doi/10.1093/mollus/eys029}, doi = {10.1093/mollus/eys029}, issn = {0260-1230, 1464-3766}, year = {2013}, date = {2013-02-01}, urldate = {2020-08-18}, journal = {Journal of Molluscan Studies}, volume = {79}, number = {1}, pages = {76--78}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Papadakis, I E; Kentouri, M; Divanach, P; Mylonas, C C Aquaculture, 388-391 (1), pp. 76–88, 2013, ISSN: 00448486, (Publisher: Elsevier B.V.). @article{papadakis_ontogeny_2013, title = {Ontogeny of the digestive system of meagre Argyrosomus regius reared in a mesocosm, and quantitative changes of lipids in the liver from hatching to juvenile}, author = {I E Papadakis and M Kentouri and P Divanach and C C Mylonas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84873700252&doi=10.1016%2fj.aquaculture.2013.01.012&partnerID=40&md5=f4c62c1417bb80d113e957f7d9a763ac}, doi = {10.1016/j.aquaculture.2013.01.012}, issn = {00448486}, year = {2013}, date = {2013-01-01}, journal = {Aquaculture}, volume = {388-391}, number = {1}, pages = {76--88}, abstract = {Histological development of the digestive systemwas studied in association with feeding preferences in meagre (Argyrosomus regius) fromhatching to juvenile (44 days after hatching, dah), using amesocosmsystem. In addition, the liver lipid content was evaluated using histological methods (area covered with lipid vacuoles, ACLV%). The ontogeny of the digestive system was completed by 19 dah (or 444 degree days) with the gastric glands appearing at 15 dah (361 degree days), the pyloric ceaca at 17 dah (404 degree days) and the Y-shaped stomach formed at 19 dah (444 degree days). The rearing period was characterized first by relatively slow growth until tail flexion ( 15 dah) and fast growth thereafter (mean±SD total length of 45.14±4.00 mm at 44 dah). When the feeding protocol included exclusively rotifers, mean ACLV remained low (2.39±0.34%) while feeding on Artemia nauplii and copepods increased liver ACLV to 47.18±6.56% at 20 dah. Changes in the feeding protocol were reflected in feeding preferences (stomach content), and variations of liver lipid content and the occurrence of vacuoles in the intestine. During transition from live prey to artificial feed ( 28 dah), ACLV decreased significantly - indicating amalnutrition period - concomitantwith a delay in the acceptance of artificial feed of 8 days. Thereafter, consumption of artificial feed resulted in an increased ACLV to 56.3±7.6% at 36 dah. The results indicate that during early development meagre is a fast growing species, developing rapidly the structures and basic organs of the digestive system required to overcome successfully the critical stages of larval rearing. The study also shows that histological evaluation of liver lipid content using the ACLV may be a valuable tool incommercial aquaculture to improve larval rearing protocols, and production efficiency. © 2013 Elsevier B.V.}, note = {Publisher: Elsevier B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Histological development of the digestive systemwas studied in association with feeding preferences in meagre (Argyrosomus regius) fromhatching to juvenile (44 days after hatching, dah), using amesocosmsystem. In addition, the liver lipid content was evaluated using histological methods (area covered with lipid vacuoles, ACLV%). The ontogeny of the digestive system was completed by 19 dah (or 444 degree days) with the gastric glands appearing at 15 dah (361 degree days), the pyloric ceaca at 17 dah (404 degree days) and the Y-shaped stomach formed at 19 dah (444 degree days). The rearing period was characterized first by relatively slow growth until tail flexion ( 15 dah) and fast growth thereafter (mean±SD total length of 45.14±4.00 mm at 44 dah). When the feeding protocol included exclusively rotifers, mean ACLV remained low (2.39±0.34%) while feeding on Artemia nauplii and copepods increased liver ACLV to 47.18±6.56% at 20 dah. Changes in the feeding protocol were reflected in feeding preferences (stomach content), and variations of liver lipid content and the occurrence of vacuoles in the intestine. During transition from live prey to artificial feed ( 28 dah), ACLV decreased significantly - indicating amalnutrition period - concomitantwith a delay in the acceptance of artificial feed of 8 days. Thereafter, consumption of artificial feed resulted in an increased ACLV to 56.3±7.6% at 36 dah. The results indicate that during early development meagre is a fast growing species, developing rapidly the structures and basic organs of the digestive system required to overcome successfully the critical stages of larval rearing. The study also shows that histological evaluation of liver lipid content using the ACLV may be a valuable tool incommercial aquaculture to improve larval rearing protocols, and production efficiency. © 2013 Elsevier B.V. |
Georgakopoulou, E; Loizides, M; Christou, M; Iliopoulou, M; Papadakis, I; Katharios, P; Divanach, P; Koumoundouros, G Thermally-induced phenotypic plasticity in gilthead sea bream. Journal Article Communications in agricultural and applied biological sciences, 78 (4), pp. 143–146, 2013, ISSN: 13791176. @article{georgakopoulou_thermally-induced_2013, title = {Thermally-induced phenotypic plasticity in gilthead sea bream.}, author = {E Georgakopoulou and M Loizides and M Christou and M Iliopoulou and I Papadakis and P Katharios and P Divanach and G Koumoundouros}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84907271903&partnerID=40&md5=fc2e59b41c153fa8c094c3eb304c3191}, issn = {13791176}, year = {2013}, date = {2013-01-01}, journal = {Communications in agricultural and applied biological sciences}, volume = {78}, number = {4}, pages = {143--146}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Kostopoulou, V; Tsopelakos, A; Zogopoulou, E; Miliou, H; Divanach, P; Katharios, P Communications in agricultural and applied biological sciences, 78 (4), pp. 219–222, 2013, ISSN: 13791176. @article{kostopoulou_effect_2013, title = {The effect of different phytoplankton species and commercial enrichment products on the fatty acid profile, enzyme activity, and overall condition of the rotifer Brachionus Plicatilis.}, author = {V Kostopoulou and A Tsopelakos and E Zogopoulou and H Miliou and P Divanach and P Katharios}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84907278292&partnerID=40&md5=a21b495ca8214710c394fe4eff5e1fba}, issn = {13791176}, year = {2013}, date = {2013-01-01}, journal = {Communications in agricultural and applied biological sciences}, volume = {78}, number = {4}, pages = {219--222}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Neofytou, M; Somarakis, S; Papadakis, V; Divanach, P; Sterioti, A; Kentouri, M Journal of Biological Research (Greece), 19 , pp. 99 – 110, 2013. @article{neofytou_effect_2013, title = {Effect of temperature, stocking density, feeding conditions and experimental day on the horizontal and vertical distribution of sea bass fry Dicentrarchus labrax (Linnaeus, 1758)}, author = {M Neofytou and S Somarakis and V Papadakis and P Divanach and A Sterioti and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84875945263&partnerID=40&md5=1fa1417692f1382db98a707c27d11da4}, year = {2013}, date = {2013-01-01}, journal = {Journal of Biological Research (Greece)}, volume = {19}, pages = {99 -- 110}, abstract = {The working hypothesis of the present study is to verify whether, the spatial distribution of sea bass fry remains invariable under the pressure of certain rearing conditions, due to its extended plasticity or, certain situations lead to specific distributions. for this reason, we investigated and compared horizontal and vertical distribution of sea bass reared in aquaria, at three different stocking densities (5 ind. l-1, 10 ind. l-1 and 20 ind. l-1), two different temperatures (16 and 23°C) and two different feeding conditions (feeding fish and starving fish). the analysis of the video records and General Linear Models revealed a strong effect of stocking density, feeding condition, and day of experimentation on fish behavior interpreted by the vertical and horizontal distribution of fish. temperature also affected distribution when it was combined with stocking density and feeding condition. however, this effect seemed to be lessened by the effect of the other variables (i.e. stocking density, feeding condition and day of experimentation). the exposure time (chronic stress) to the stocking density, temperature and feeding condition as well as to their additive cumulative effect (variables interactions) was the cause of the development of stereotypic behaviors that increased in occurrence frequency and over an experimental time lapse and gradually led to the weakening and death of the fry (bad welfare). thus, it is assumed that stocking density, feeding condition, day of experimentation and temperature can have negative effects on sea bass "well being" and in order to maintain its welfare during rearing it is important to take all necessary precautions of handling when dealing with these variables in the fish environment.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The working hypothesis of the present study is to verify whether, the spatial distribution of sea bass fry remains invariable under the pressure of certain rearing conditions, due to its extended plasticity or, certain situations lead to specific distributions. for this reason, we investigated and compared horizontal and vertical distribution of sea bass reared in aquaria, at three different stocking densities (5 ind. l-1, 10 ind. l-1 and 20 ind. l-1), two different temperatures (16 and 23°C) and two different feeding conditions (feeding fish and starving fish). the analysis of the video records and General Linear Models revealed a strong effect of stocking density, feeding condition, and day of experimentation on fish behavior interpreted by the vertical and horizontal distribution of fish. temperature also affected distribution when it was combined with stocking density and feeding condition. however, this effect seemed to be lessened by the effect of the other variables (i.e. stocking density, feeding condition and day of experimentation). the exposure time (chronic stress) to the stocking density, temperature and feeding condition as well as to their additive cumulative effect (variables interactions) was the cause of the development of stereotypic behaviors that increased in occurrence frequency and over an experimental time lapse and gradually led to the weakening and death of the fry (bad welfare). thus, it is assumed that stocking density, feeding condition, day of experimentation and temperature can have negative effects on sea bass "well being" and in order to maintain its welfare during rearing it is important to take all necessary precautions of handling when dealing with these variables in the fish environment. |
Makridis, P; Papandroulakis, N; Divanach, P Use of Phaeobacter sp. probiotic bacteria for the rearing of sea bass larvae (Dicentrarchus labrax). Journal Article Communications in agricultural and applied biological sciences, 78 (4), pp. 259–261, 2013, ISSN: 13791176. @article{makridis_use_2013, title = {Use of Phaeobacter sp. probiotic bacteria for the rearing of sea bass larvae (Dicentrarchus labrax).}, author = {P Makridis and N Papandroulakis and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84907261002&partnerID=40&md5=2d43cf01b17d910e6d0c1e4186e768bd}, issn = {13791176}, year = {2013}, date = {2013-01-01}, journal = {Communications in agricultural and applied biological sciences}, volume = {78}, number = {4}, pages = {259--261}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2012 |
Makridis, P; Ferreira, T; Kokou, F; Tsigenopoulos, C S; Divanach, P Quantitative and Qualitative Aspects of Bacterial Communities Associated with Cultures of Chlorella minutissima Journal Article Journal of the World Aquaculture Society, 43 , pp. 571 – 578, 2012. @article{makridis_quantitative_2012, title = {Quantitative and Qualitative Aspects of Bacterial Communities Associated with Cultures of Chlorella minutissima}, author = {P Makridis and T Ferreira and F Kokou and C S Tsigenopoulos and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84864765348&partnerID=40&md5=24dbfa072f09304f0a5ae3c91e329aab}, year = {2012}, date = {2012-01-01}, journal = {Journal of the World Aquaculture Society}, volume = {43}, pages = {571 -- 578}, abstract = {Microbiological conditions in 200 L cultures of microalgae Chlorella minutissima in polyethylene sleeves were examined. The influence of addition of antibiotic (nitrofurantoin) was studied. Samples were taken 2, 5, 9, and 14 d after inoculation and were spread on solid Zobell medium and on thiosulfate citrate bile salts sucrose agar for the enumeration of total numbers of bacteria and numbers of presumptive Vibrio bacteria, respectively. Microalgae grew well in the cultures and high numbers of culturable bacteria (about 107 bacteria/mL) were present in all microalgae cultures. Addition of antibiotic in 200-L cultures of C. minutissima did not result in a significant decrease of total counts of bacteria per unit volume (P > 0.05), while the numbers of presumptive Vibrio were higher in cultures added antibiotic compared with the control treatment (P < 0.05). The numbers of presumptive Vibrio per unit volume and the percentage of fast-growing bacteria were higher in microalgae supernatant than in noncentrifuged samples. Representative colonies were taken from all samples and in total 649 bacterial strains were isolated during this study. A range of phylotypes was identified by amplification and sequencing of the 16S rDNA gene and phylogenetic trees were constructed. Several members of the Roseobacter clade were dominant among the culturable isolates. © Copyright by the World Aquaculture Society 2012.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Microbiological conditions in 200 L cultures of microalgae Chlorella minutissima in polyethylene sleeves were examined. The influence of addition of antibiotic (nitrofurantoin) was studied. Samples were taken 2, 5, 9, and 14 d after inoculation and were spread on solid Zobell medium and on thiosulfate citrate bile salts sucrose agar for the enumeration of total numbers of bacteria and numbers of presumptive Vibrio bacteria, respectively. Microalgae grew well in the cultures and high numbers of culturable bacteria (about 107 bacteria/mL) were present in all microalgae cultures. Addition of antibiotic in 200-L cultures of C. minutissima did not result in a significant decrease of total counts of bacteria per unit volume (P > 0.05), while the numbers of presumptive Vibrio were higher in cultures added antibiotic compared with the control treatment (P < 0.05). The numbers of presumptive Vibrio per unit volume and the percentage of fast-growing bacteria were higher in microalgae supernatant than in noncentrifuged samples. Representative colonies were taken from all samples and in total 649 bacterial strains were isolated during this study. A range of phylotypes was identified by amplification and sequencing of the 16S rDNA gene and phylogenetic trees were constructed. Several members of the Roseobacter clade were dominant among the culturable isolates. © Copyright by the World Aquaculture Society 2012. |
Chatzifotis, S; Panagiotidou, M; Divanach, P Effect of protein and lipid dietary levels on the growth of juvenile meagre (Argyrosomus regius) Journal Article Aquaculture International, 20 (1), pp. 91–98, 2012, ISSN: 09676120. @article{chatzifotis_effect_2012, title = {Effect of protein and lipid dietary levels on the growth of juvenile meagre (Argyrosomus regius)}, author = {S Chatzifotis and M Panagiotidou and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84855968322&doi=10.1007%2fs10499-011-9443-y&partnerID=40&md5=452601ee09bed5f72bc5ea3876ab251e}, doi = {10.1007/s10499-011-9443-y}, issn = {09676120}, year = {2012}, date = {2012-01-01}, journal = {Aquaculture International}, volume = {20}, number = {1}, pages = {91--98}, abstract = {This study investigates the effects of dietary lipid and protein levels in the growth, feed utilization and body composition of meagre (Argyrosomus regius). Triplicate groups of juvenile fish (23. 4 ± 4. 9 g average weight ± SD) were fed four isolipidic diets (17. 5% crude lipids) containing 40, 45, 50 and 54% of protein, while in a separate experiment, fish (21. 8 ± 3. 7 g average weight ± SD) were fed four isonitrogenous (50% crude protein, dry matter) each containing 12, 15, 17 or 20% of crude lipids. In the protein requirement experiment, the increase in crude protein in the diet from 40 up to 50% positively affected SGR (Specific growth rate) and FCR (Feed conversion ratio). The increase in crude lipids in the diet from 12 up to 17% showed a tendency for improvement in SGR and FCR. In both experiments, the fastest growing fish exhibited higher lipid depots. Overall, the present investigation showed that juvenile meagre has an estimated protein requirement of 50% and it does not seem to require high dietary lipid levels. © 2011 Springer Science+Business Media B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study investigates the effects of dietary lipid and protein levels in the growth, feed utilization and body composition of meagre (Argyrosomus regius). Triplicate groups of juvenile fish (23. 4 ± 4. 9 g average weight ± SD) were fed four isolipidic diets (17. 5% crude lipids) containing 40, 45, 50 and 54% of protein, while in a separate experiment, fish (21. 8 ± 3. 7 g average weight ± SD) were fed four isonitrogenous (50% crude protein, dry matter) each containing 12, 15, 17 or 20% of crude lipids. In the protein requirement experiment, the increase in crude protein in the diet from 40 up to 50% positively affected SGR (Specific growth rate) and FCR (Feed conversion ratio). The increase in crude lipids in the diet from 12 up to 17% showed a tendency for improvement in SGR and FCR. In both experiments, the fastest growing fish exhibited higher lipid depots. Overall, the present investigation showed that juvenile meagre has an estimated protein requirement of 50% and it does not seem to require high dietary lipid levels. © 2011 Springer Science+Business Media B.V. |
Kokou, F; Makridis, P; Kentouri, M; Divanach, P Antibacterial activity in microalgae cultures Journal Article Aquaculture Research, 43 , pp. 1520 – 1527, 2012. @article{kokou_antibacterial_2012, title = {Antibacterial activity in microalgae cultures}, author = {F Kokou and P Makridis and M Kentouri and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84865858405&partnerID=40&md5=81655dac592229e01c3b9c73ab44ce38}, year = {2012}, date = {2012-01-01}, journal = {Aquaculture Research}, volume = {43}, pages = {1520 -- 1527}, abstract = {Five cultures of microalgae (Chlorella minutissima, Tetraselmis chui, Nannochloropsis sp., Arthrospira platensis and Isochrysis sp.) with no culturable bacteria were tested for their ability to inhibit the growth of six Vibrio bacterial strains (V. parahaemolyticus, V. anguillarum, V. splendidus, V. scophthalmi, V. alginolyticus and V. lentus). The influence of light on the antibacterial activity of the microalgae was investigated. All microalgae cultures inhibited the growth of bacteria compared with the control treatments (P textless 0.05), and their antibacterial activity was not influenced by the presence or absence of light. In the control groups, the numbers of bacteria increased exponentially during the experimental period in the absence of microalgae cells demonstrating that the bacterial cells were able to utilize the growth medium of microalgae cultures. The present results may explain the low levels or absence of Vibrio strains in microalgae cultures, and the positive effect of addition of microalgae in rearing of fish larvae, and implicate the production of antibacterial compounds by microalgae cells. © 2011 Blackwell Publishing Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Five cultures of microalgae (Chlorella minutissima, Tetraselmis chui, Nannochloropsis sp., Arthrospira platensis and Isochrysis sp.) with no culturable bacteria were tested for their ability to inhibit the growth of six Vibrio bacterial strains (V. parahaemolyticus, V. anguillarum, V. splendidus, V. scophthalmi, V. alginolyticus and V. lentus). The influence of light on the antibacterial activity of the microalgae was investigated. All microalgae cultures inhibited the growth of bacteria compared with the control treatments (P textless 0.05), and their antibacterial activity was not influenced by the presence or absence of light. In the control groups, the numbers of bacteria increased exponentially during the experimental period in the absence of microalgae cells demonstrating that the bacterial cells were able to utilize the growth medium of microalgae cultures. The present results may explain the low levels or absence of Vibrio strains in microalgae cultures, and the positive effect of addition of microalgae in rearing of fish larvae, and implicate the production of antibacterial compounds by microalgae cells. © 2011 Blackwell Publishing Ltd. |
Kostopoulou, Venetia; Vasilakis, Manolis; Divanach, Pascal Semi-continuous mass culture of rotifers (Brachionus plicatilis) using an automatic feeder Journal Article Aquaculture Research, 43 (1), pp. 91–98, 2012, ISSN: 1355557X. @article{kostopoulou_semi-continuous_2012, title = {Semi-continuous mass culture of rotifers (Brachionus plicatilis) using an automatic feeder}, author = {Venetia Kostopoulou and Manolis Vasilakis and Pascal Divanach}, url = {http://doi.wiley.com/10.1111/j.1365-2109.2011.02807.x}, doi = {10.1111/j.1365-2109.2011.02807.x}, issn = {1355557X}, year = {2012}, date = {2012-01-01}, urldate = {2020-08-18}, journal = {Aquaculture Research}, volume = {43}, number = {1}, pages = {91--98}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2011 |
Doxa, Chrisa K; Papadakis, Ioannis E; Divanach, Pascal; Kentouri, Maroudio Aquaculture Research, 42 (11), pp. 1623–1631, 2011, ISSN: 1355557X. @article{doxa_effect_2011, title = {Effect of feeding delay after self-feeder activation on growth performance and feeding behaviour of red porgy (Pagrus pagrus L. 1758): application to submergible cages with surface hopper: Feeding behaviour of red porgy}, author = {Chrisa K Doxa and Ioannis E Papadakis and Pascal Divanach and Maroudio Kentouri}, url = {http://doi.wiley.com/10.1111/j.1365-2109.2010.02753.x}, doi = {10.1111/j.1365-2109.2010.02753.x}, issn = {1355557X}, year = {2011}, date = {2011-10-01}, urldate = {2020-08-18}, journal = {Aquaculture Research}, volume = {42}, number = {11}, pages = {1623--1631}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Vardanis, George; Sfichi-Duke, Liliana; Tort, Lluis; Divanach, Pascal; Kotzabasis, Kiriakos; Pavlidis, Michail Aquaculture Research, 42 (3), pp. 341–350, 2011, ISSN: 1355557X. @article{vardanis_use_2011, title = {The use of biochemical, sensorial and chromaticity attributes as indicators of postmortem changes in commercial-size, cultured red porgy Pagrus pagrus, stored on ice: Freshness indicators in red porgy}, author = {George Vardanis and Liliana Sfichi-Duke and Lluis Tort and Pascal Divanach and Kiriakos Kotzabasis and Michail Pavlidis}, url = {http://doi.wiley.com/10.1111/j.1365-2109.2010.02628.x}, doi = {10.1111/j.1365-2109.2010.02628.x}, issn = {1355557X}, year = {2011}, date = {2011-02-01}, urldate = {2020-08-18}, journal = {Aquaculture Research}, volume = {42}, number = {3}, pages = {341--350}, abstract = {The freshness of red porgy slaughtered in ice slurry and stored in melting ice was evaluated instrumentally, biochemically and sensorialy. Additionally, postmortem skin colour changes were monitored, in an attempt to demonstrate the use of chromaticity parameters as a reliable and convenient approach to quality assessment. Dielectric properties showed a statistically significant decrease (P<0.05) from 12±0 on day 0 to 7.85±0.31 on day 7; there was also a significant decrease in muscle polyamines with more than two amino groups (spermidine and spermine) and an increase in the di-amine putrescine. The sensorial score was significantly decreased from 30±0 to 4.33±0.21 on day 7. Minimal lightness (L*) and hue (H°ab) at the dorsal skin area were observed on days 1 and 3 following harvesting. Finally, there was a marked decrease in the entire colour index (ECI - a combination of skin hue and chroma) of both the dorsal and the ventral area in day 3 onwards to day 7, as well as a statistically significant correlation between all the estimated freshness indices and ECI. Based on all these, we inferred that the total polyamines (or putrescine to spermidine and spermine ratio) and ECI could be reliable estimators of freshness, at least under the experimental conditions applied. © 2010 The Authors. Aquaculture Research © 2010 Blackwell Publishing Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The freshness of red porgy slaughtered in ice slurry and stored in melting ice was evaluated instrumentally, biochemically and sensorialy. Additionally, postmortem skin colour changes were monitored, in an attempt to demonstrate the use of chromaticity parameters as a reliable and convenient approach to quality assessment. Dielectric properties showed a statistically significant decrease (P<0.05) from 12±0 on day 0 to 7.85±0.31 on day 7; there was also a significant decrease in muscle polyamines with more than two amino groups (spermidine and spermine) and an increase in the di-amine putrescine. The sensorial score was significantly decreased from 30±0 to 4.33±0.21 on day 7. Minimal lightness (L*) and hue (H°ab) at the dorsal skin area were observed on days 1 and 3 following harvesting. Finally, there was a marked decrease in the entire colour index (ECI - a combination of skin hue and chroma) of both the dorsal and the ventral area in day 3 onwards to day 7, as well as a statistically significant correlation between all the estimated freshness indices and ECI. Based on all these, we inferred that the total polyamines (or putrescine to spermidine and spermine ratio) and ECI could be reliable estimators of freshness, at least under the experimental conditions applied. © 2010 The Authors. Aquaculture Research © 2010 Blackwell Publishing Ltd. |
Katharios, P; Rigos, G; Divanach, P Enteromyxum leei (Myxozoa), a Lethal Intruder of Tropical Pet Fish: First Case in Humphead Wrasse, Cheilinus undulatus (Rüppell, 1835) Journal Article Journal of Exotic Pet Medicine, 20 (2), pp. 138–143, 2011, ISSN: 15575063. @article{katharios_enteromyxum_2011, title = {Enteromyxum leei (Myxozoa), a Lethal Intruder of Tropical Pet Fish: First Case in Humphead Wrasse, Cheilinus undulatus (Rüppell, 1835)}, author = {P Katharios and G Rigos and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79955634789&doi=10.1053%2fj.jepm.2011.02.009&partnerID=40&md5=e3c00ff0668cec7f9df0c2a21f5fd013}, doi = {10.1053/j.jepm.2011.02.009}, issn = {15575063}, year = {2011}, date = {2011-01-01}, journal = {Journal of Exotic Pet Medicine}, volume = {20}, number = {2}, pages = {138--143}, abstract = {The lethal effect of enteromyxosis is recorded for the first time in the humphead wrasse, Cheilinus undulatus, a tropical pet fish. Enteromyxosis was caused by the myxosporean parasite Enteromyxum leei, and the gut was the only tissue affected in this case. Severe enteritis and degeneration of the intestinal mucosa may have caused a digestive function disorder, thereby resulting in the fish's death. The histopathological findings of this case included destruction of normal tissue architecture of the intestinal mucosa, chronic inflammation, and infiltration of mast cells/eosinophilic granular cells, which resembled findings described in other fish that were diagnosed with enteromyxosis. Induced stress by transportation and entrance to a new environment possibly played an underlying role in the virulence of the parasitic infection and associated tissue pathology. © 2011 Elsevier Inc.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The lethal effect of enteromyxosis is recorded for the first time in the humphead wrasse, Cheilinus undulatus, a tropical pet fish. Enteromyxosis was caused by the myxosporean parasite Enteromyxum leei, and the gut was the only tissue affected in this case. Severe enteritis and degeneration of the intestinal mucosa may have caused a digestive function disorder, thereby resulting in the fish's death. The histopathological findings of this case included destruction of normal tissue architecture of the intestinal mucosa, chronic inflammation, and infiltration of mast cells/eosinophilic granular cells, which resembled findings described in other fish that were diagnosed with enteromyxosis. Induced stress by transportation and entrance to a new environment possibly played an underlying role in the virulence of the parasitic infection and associated tissue pathology. © 2011 Elsevier Inc. |
Chatzifotis, S; Juan, Vaz I; Kyriazi, P; Divanach, P; Pavlidis, M Dietary carotenoids and skin melanin content influence the coloration of farmed red porgy (Pagrus pagrus) Journal Article Aquaculture Nutrition, 17 (2), pp. e90–e100, 2011, ISSN: 13535773. @article{chatzifotis_dietary_2011, title = {Dietary carotenoids and skin melanin content influence the coloration of farmed red porgy (Pagrus pagrus)}, author = {S Chatzifotis and I Vaz Juan and P Kyriazi and P Divanach and M Pavlidis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79952572119&doi=10.1111%2fj.1365-2095.2009.00738.x&partnerID=40&md5=c1612c2e4e5ab3ab429a90e0a18c1eb2}, doi = {10.1111/j.1365-2095.2009.00738.x}, issn = {13535773}, year = {2011}, date = {2011-01-01}, journal = {Aquaculture Nutrition}, volume = {17}, number = {2}, pages = {e90--e100}, abstract = {The present study investigates the effects of dietary carotenoid sources on the coloration of the red porgy, Pagrus pagrus. Red porgies (131.9±16.2g; mean±SD) were fed for 12weeks on five different diets supplemented with red carotenoids (mainly astaxanthin esters) supplied from Haematococcus pluvialis algae and yellow carotenoids (mainly β-carotene, lutein, and zeaxanthin) supplied from Alfalfa, Medicago sativa L. and Spirulina, Spirulina pacifica. The carotenoid-supplemented diets did not have any marked effect on the growth rate, the feed conversion ratio, the daily feeding rate, or the hepatosomatic index of red porgy. The biochemical indices measured in plasma including cholesterol, total proteins, glucose, lactate, phospholipids, non-esterified fatty acids, triglycerides, and thyroid hormones did not differ significantly among groups. Diet did not affect significantly the melanophore-area coverage, the melanin skin concentration and skin lightness. Carotenoid-supplemented diets affected significantly the carotenoid deposition in the skin, the presence and distribution of erythorphores and xanthophores, and skin hue and chroma. Overall, data have shown the efficacy of Haematococcus algae in promoting a reddish coloration in red porgy. © 2009 Blackwell Publishing Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The present study investigates the effects of dietary carotenoid sources on the coloration of the red porgy, Pagrus pagrus. Red porgies (131.9±16.2g; mean±SD) were fed for 12weeks on five different diets supplemented with red carotenoids (mainly astaxanthin esters) supplied from Haematococcus pluvialis algae and yellow carotenoids (mainly β-carotene, lutein, and zeaxanthin) supplied from Alfalfa, Medicago sativa L. and Spirulina, Spirulina pacifica. The carotenoid-supplemented diets did not have any marked effect on the growth rate, the feed conversion ratio, the daily feeding rate, or the hepatosomatic index of red porgy. The biochemical indices measured in plasma including cholesterol, total proteins, glucose, lactate, phospholipids, non-esterified fatty acids, triglycerides, and thyroid hormones did not differ significantly among groups. Diet did not affect significantly the melanophore-area coverage, the melanin skin concentration and skin lightness. Carotenoid-supplemented diets affected significantly the carotenoid deposition in the skin, the presence and distribution of erythorphores and xanthophores, and skin hue and chroma. Overall, data have shown the efficacy of Haematococcus algae in promoting a reddish coloration in red porgy. © 2009 Blackwell Publishing Ltd. |
Katharios, P; Papadaki, M; Ternengo, S; Kantham, P K; Zeri, C; Petraki, P E; Divanach, P Chronic ulcerative dermatopathy in cultured marine fishes. Comparative study in sharpsnout sea bream, Diplodus puntazzo (Walbaum) Journal Article Journal of Fish Diseases, 34 (6), pp. 459–474, 2011, ISSN: 01407775. @article{katharios_chronic_2011, title = {Chronic ulcerative dermatopathy in cultured marine fishes. Comparative study in sharpsnout sea bream, Diplodus puntazzo (Walbaum)}, author = {P Katharios and M Papadaki and S Ternengo and P K Kantham and C Zeri and P E Petraki and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79955640274&doi=10.1111%2fj.1365-2761.2011.01257.x&partnerID=40&md5=da9a133e20c28b1376b9ba550b57d497}, doi = {10.1111/j.1365-2761.2011.01257.x}, issn = {01407775}, year = {2011}, date = {2011-01-01}, journal = {Journal of Fish Diseases}, volume = {34}, number = {6}, pages = {459--474}, abstract = {Chronic ulcerative dermatopathy (CUD) also known as chronic erosive dermatopathy, hole-in-the-head, head and lateral line erosion syndrome (HLLE) and lateral line depigmentation (LLD) is a chronic disease of unknown aetiology that affects the lateral line canals of the head and the trunk of various fish species. It has been described only in freshwater species although there are reports that it also affects marine fish. Here, we describe the disease in cultured sharpsnout sea bream using histology and scanning electron microscopy and identify several marine species as CUD sensitive. The results of this study correlate the development of the disease with the use of borehole water, indicating that the aetiology is probably associated with water quality rather than nutritional imbalance or infectious agents. © 2011 Blackwell Publishing Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Chronic ulcerative dermatopathy (CUD) also known as chronic erosive dermatopathy, hole-in-the-head, head and lateral line erosion syndrome (HLLE) and lateral line depigmentation (LLD) is a chronic disease of unknown aetiology that affects the lateral line canals of the head and the trunk of various fish species. It has been described only in freshwater species although there are reports that it also affects marine fish. Here, we describe the disease in cultured sharpsnout sea bream using histology and scanning electron microscopy and identify several marine species as CUD sensitive. The results of this study correlate the development of the disease with the use of borehole water, indicating that the aetiology is probably associated with water quality rather than nutritional imbalance or infectious agents. © 2011 Blackwell Publishing Ltd. |
Doxa, C K; Sterioti, A; Kentouri, M; Divanach, P Encapsulated development of the marine gastropod Tonna galea (Linnaeus, 1758) in captivity Journal Article Journal of Biological Research, 16 , pp. 304 – 307, 2011. @article{doxa_encapsulated_2011, title = {Encapsulated development of the marine gastropod Tonna galea (Linnaeus, 1758) in captivity}, author = {C K Doxa and A Sterioti and M Kentouri and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-80052546780&partnerID=40&md5=e2d86fb176ffda6ce56ee10b2c701ef2}, year = {2011}, date = {2011-01-01}, journal = {Journal of Biological Research}, volume = {16}, pages = {304 -- 307}, abstract = {In the present study the reproductive biology of Tonna galea (Linnaeus, 1758) was studied for the first time. In mid-September 2006 one individual was found laying a pale pink egg rosette of 39.5 cm length. Number of embryos, stages of development, shape and dimensions were studied in relation to time and measured on microphotographs of randomly sampled capsules. Each oval or spherical shaped capsule of 3.61 mm total length contained 101 developing embryos. The embryo diameter ranged from 297.5 μm of the unsegmented egg to 489 μm of the free veliger. At 21°C eclosion occurred 34 days after capsule deposition, at a free-swimming veliger stage. The duration of each developmental stage, from one cell to veliger, is reported. Results are discussed in relation to possible culture and use for ecological purposes.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In the present study the reproductive biology of Tonna galea (Linnaeus, 1758) was studied for the first time. In mid-September 2006 one individual was found laying a pale pink egg rosette of 39.5 cm length. Number of embryos, stages of development, shape and dimensions were studied in relation to time and measured on microphotographs of randomly sampled capsules. Each oval or spherical shaped capsule of 3.61 mm total length contained 101 developing embryos. The embryo diameter ranged from 297.5 μm of the unsegmented egg to 489 μm of the free veliger. At 21°C eclosion occurred 34 days after capsule deposition, at a free-swimming veliger stage. The duration of each developmental stage, from one cell to veliger, is reported. Results are discussed in relation to possible culture and use for ecological purposes. |
P., Kokkinias; I., Georga; I.E., Papadakis; P., Katharios; C., Batargias; P., Divanach; G., Koumoundouros Effect of early temperature on the osteological morphometry of European sea bass juveniles. Inproceedings EAS, (Ed.): pp. 564–565, Rhodes, Greece, 2011. @inproceedings{kokkinias_effect_2011, title = {Effect of early temperature on the osteological morphometry of European sea bass juveniles.}, author = {Kokkinias P. and Georga I. and Papadakis I.E. and Katharios P. and Batargias C. and Divanach P. and Koumoundouros G.}, editor = {EAS}, year = {2011}, date = {2011-01-01}, pages = {564--565}, address = {Rhodes, Greece}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
2010 |
Laggis, A; Sfakianakis, D G; Divanach, P; Kentouri, M Ontogeny of the body skeleton in Seriola dumerili (Risso, 1810) Journal Article Italian Journal of Zoology, 77 (3), pp. 303–315, 2010, ISSN: 1125-0003, 1748-5851. @article{laggis_ontogeny_2010, title = {Ontogeny of the body skeleton in Seriola dumerili (Risso, 1810)}, author = {A Laggis and D G Sfakianakis and P Divanach and M Kentouri}, url = {http://www.tandfonline.com/doi/abs/10.1080/11250000903170870}, doi = {10.1080/11250000903170870}, issn = {1125-0003, 1748-5851}, year = {2010}, date = {2010-08-01}, urldate = {2020-08-18}, journal = {Italian Journal of Zoology}, volume = {77}, number = {3}, pages = {303--315}, abstract = {Osteological development of Seriola dumerili is described and illustrated based on 141 specimens (3.9-27.0 mm TL) reared under semi-intensive culture conditions (Greece). The ontogeny of the vertebral column started with the formation of the neural arches (4.6 mm TL) and continued with the haemal arches and the centrum (4.8 mm TL). Until the end of the study, the centrum (12.5 mm TL) and neural arches (23.8 mm TL) were fully formed, while the haemal arches were not. The first skeletal elements of the fins started developing in the pectoral (3.9 mm TL), anal (4.8 mm TL), caudal (4.8 mm TL), dorsal (6.0 mm TL) and pelvic fins (6.3 mm TL), while the fin rays followed a slightly different pattern pectoral (5.6 mm TL), caudal (5.3 mm TL), anal (6.2 mm TL), dorsal (6.3 mm TL) and pelvic fin rays (7.0 mm TL). The ontogeny of the skeletal parts are compared with those of other Carangidae and Teleosts. © 2010 Unione Zoologica Italiana.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Osteological development of Seriola dumerili is described and illustrated based on 141 specimens (3.9-27.0 mm TL) reared under semi-intensive culture conditions (Greece). The ontogeny of the vertebral column started with the formation of the neural arches (4.6 mm TL) and continued with the haemal arches and the centrum (4.8 mm TL). Until the end of the study, the centrum (12.5 mm TL) and neural arches (23.8 mm TL) were fully formed, while the haemal arches were not. The first skeletal elements of the fins started developing in the pectoral (3.9 mm TL), anal (4.8 mm TL), caudal (4.8 mm TL), dorsal (6.0 mm TL) and pelvic fins (6.3 mm TL), while the fin rays followed a slightly different pattern pectoral (5.6 mm TL), caudal (5.3 mm TL), anal (6.2 mm TL), dorsal (6.3 mm TL) and pelvic fin rays (7.0 mm TL). The ontogeny of the skeletal parts are compared with those of other Carangidae and Teleosts. © 2010 Unione Zoologica Italiana. |
Papazi, Aikaterini; Makridis, Pavlos; Divanach, Pascal Harvesting Chlorella minutissima using cell coagulants Journal Article Journal of Applied Phycology, 22 (3), pp. 349–355, 2010, ISSN: 0921-8971, 1573-5176. @article{papazi_harvesting_2010, title = {Harvesting Chlorella minutissima using cell coagulants}, author = {Aikaterini Papazi and Pavlos Makridis and Pascal Divanach}, url = {http://link.springer.com/10.1007/s10811-009-9465-2}, doi = {10.1007/s10811-009-9465-2}, issn = {0921-8971, 1573-5176}, year = {2010}, date = {2010-06-01}, urldate = {2020-08-18}, journal = {Journal of Applied Phycology}, volume = {22}, number = {3}, pages = {349--355}, abstract = {Twelve salts were tested for their ability to coagulate microalgae cells in cultures of Chlorella minutissima. The final aim was to develop an easy and efficient approach for harvesting microalgae biomass in dense cultures. Aluminum, ferric, and zinc salts coagulated C. minutissima cultures, while optimum concentration was 0.75 and 0.5 g L-1 for sulfate and chloride salts, respectively. Aluminum salts were most efficient, but caused some cell lysis, which may render this approach inappropriate in some cases. Ferric and zinc salts were ranked second and third, respectively, according to their culture cell-coagulation efficiency. Ferric salts caused a change in the color of the cells, mainly at concentrations higher than 1 g L-1. Zinc salts were less harmful for the microalgal cells, but an additional problem was observed with cell aggregates adhering to the walls of the glass test tubes. Selection of the appropriate coagulant is related to the purpose of the coagulation process. © Springer Science+Business Media B.V. 2009.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Twelve salts were tested for their ability to coagulate microalgae cells in cultures of Chlorella minutissima. The final aim was to develop an easy and efficient approach for harvesting microalgae biomass in dense cultures. Aluminum, ferric, and zinc salts coagulated C. minutissima cultures, while optimum concentration was 0.75 and 0.5 g L-1 for sulfate and chloride salts, respectively. Aluminum salts were most efficient, but caused some cell lysis, which may render this approach inappropriate in some cases. Ferric and zinc salts were ranked second and third, respectively, according to their culture cell-coagulation efficiency. Ferric salts caused a change in the color of the cells, mainly at concentrations higher than 1 g L-1. Zinc salts were less harmful for the microalgal cells, but an additional problem was observed with cell aggregates adhering to the walls of the glass test tubes. Selection of the appropriate coagulant is related to the purpose of the coagulation process. © Springer Science+Business Media B.V. 2009. |
Dimitroglou, A; Davies, S J; Sweetman, J; Divanach, P; Chatzifotis, S Aquaculture Research, 41 (9), pp. e245–e251, 2010, ISSN: 1355557X. @article{dimitroglou_dietary_2010, title = {Dietary supplementation of mannan oligosaccharide on white sea bream (Diplodus sargus L.) larvae: Effects on development, gut morphology and salinity tolerance}, author = {A Dimitroglou and S J Davies and J Sweetman and P Divanach and S Chatzifotis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77955944815&doi=10.1111%2fj.1365-2109.2010.02513.x&partnerID=40&md5=00b906376b6d2064549969ba72d38709}, doi = {10.1111/j.1365-2109.2010.02513.x}, issn = {1355557X}, year = {2010}, date = {2010-01-01}, journal = {Aquaculture Research}, volume = {41}, number = {9}, pages = {e245--e251}, abstract = {The influence of dietary mannan oligosaccharide (MOS) on the development, gut integrity and quality (in respect of stamina and survivability) of white sea bream Diplodus sargus L. larvae was investigated. White sea bream larvae were held under appropriate rearing conditions and fed Artemia, enriched by A1 DHA Selco™ with the addition or absence of MOS (Bio-Mos®). The results indicated that larval growth performance and survivability were not affected by the MOS supplementation. Light microscopy revealed that MOS supplementation significantly improved the intestinal morphology by increasing the villi surface area by over 12%. Transmission electron microscopy revealed that MOS supplementation increased the microvilli length by 26% compared with the control. Salinity challenge experiments showed that MOS significantly increased larval stamina and survival in both 0 and 60 mg L-1 salinity water by 13% and 22.9% respectively. These improvements in the larval quality at the early stages of fish development are important for the efficiency of intensive hatchery production. © 2010 The Authors. Journal Compilation © 2010 Blackwell Publishing Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The influence of dietary mannan oligosaccharide (MOS) on the development, gut integrity and quality (in respect of stamina and survivability) of white sea bream Diplodus sargus L. larvae was investigated. White sea bream larvae were held under appropriate rearing conditions and fed Artemia, enriched by A1 DHA Selco™ with the addition or absence of MOS (Bio-Mos®). The results indicated that larval growth performance and survivability were not affected by the MOS supplementation. Light microscopy revealed that MOS supplementation significantly improved the intestinal morphology by increasing the villi surface area by over 12%. Transmission electron microscopy revealed that MOS supplementation increased the microvilli length by 26% compared with the control. Salinity challenge experiments showed that MOS significantly increased larval stamina and survival in both 0 and 60 mg L-1 salinity water by 13% and 22.9% respectively. These improvements in the larval quality at the early stages of fish development are important for the efficiency of intensive hatchery production. © 2010 The Authors. Journal Compilation © 2010 Blackwell Publishing Ltd. |
Georgakopoulou, E; Katharios, P; Divanach, P; Koumoundouros, G Effect of temperature on the development of skeletal deformities in Gilthead seabream (Sparus aurata Linnaeus, 1758) Journal Article Aquaculture, 308 (1-2), pp. 13–19, 2010, ISSN: 00448486. @article{georgakopoulou_effect_2010, title = {Effect of temperature on the development of skeletal deformities in Gilthead seabream (Sparus aurata Linnaeus, 1758)}, author = {E Georgakopoulou and P Katharios and P Divanach and G Koumoundouros}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77956618251&doi=10.1016%2fj.aquaculture.2010.08.006&partnerID=40&md5=0385694485812464aad46479cf10e535}, doi = {10.1016/j.aquaculture.2010.08.006}, issn = {00448486}, year = {2010}, date = {2010-01-01}, journal = {Aquaculture}, volume = {308}, number = {1-2}, pages = {13--19}, abstract = {The development of skeletal deformities is an important problem for finfish hatcheries. In the present paper, the effect of water temperature on the development of skeletal deformities in Sparus aurata was examined. Six combinations of three temperatures (16, 19, and 22°C) were applied during three ontogenetic windows: the autotrophic (embryonic and yolk-sac larval stages), the exotrophic larval (first feeding to metamorphosing larvae of 14-16. mm TL) and the juvenile (metamorphosing larvae of 14-16. mm TL to juveniles of 40-45 mm TL) periods. The results demonstrated a significant effect (ptextless 0.05) of water temperature on the development of inside folded gill-cover, haemal lordosis, as well as of mild deformities of the caudal and dorsal fin-supporting elements. The prevalence of gill-cover deformities was elevated when 16°C water temperature was applied during the autotrophic and exotrophic larval periods (50.0 ± 2.8%, mean ± SD), or only during the autotrophic period (14.0 ± 0.0%). Haemal lordosis development, the second most severe skeletal deformity, presented fluctuating response against water temperature up to 14-16. mm TL (3.0 ± 4.2 to 13.0 ± 9.9%). However, the application of 22°C during the juvenile period resulted in the lowest and less variable incidence of haemal lordosis (1.0 ± 0.0 to 5.0 ± 1.4%). The mild deformities of caudal and dorsal fins presented different responses to water temperature. The prevalence of caudal-fin deformities was elevated when 16°C temperature was applied during the exotrophic larval period (54.0 ± 8.5%), while dorsal-fin deformities were favoured when 22°C temperature was applied during the autotrophic and exotrophic phases (35.0 ± 9.9 to 39.0 ± 4.2%). In the examined thermal range, growth rate was significantly elevated with the temperature increase. Fish survival was higher in the treatments where temperature after yolk-consumption increased from 16 or 19°C to 19 or 22°C, respectively. The results are discussed in respect to the onset of ontogeny of the different skeletal elements and the need of applying different thermal conditions during the development of S. aurata. © 2010 Elsevier B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The development of skeletal deformities is an important problem for finfish hatcheries. In the present paper, the effect of water temperature on the development of skeletal deformities in Sparus aurata was examined. Six combinations of three temperatures (16, 19, and 22°C) were applied during three ontogenetic windows: the autotrophic (embryonic and yolk-sac larval stages), the exotrophic larval (first feeding to metamorphosing larvae of 14-16. mm TL) and the juvenile (metamorphosing larvae of 14-16. mm TL to juveniles of 40-45 mm TL) periods. The results demonstrated a significant effect (ptextless 0.05) of water temperature on the development of inside folded gill-cover, haemal lordosis, as well as of mild deformities of the caudal and dorsal fin-supporting elements. The prevalence of gill-cover deformities was elevated when 16°C water temperature was applied during the autotrophic and exotrophic larval periods (50.0 ± 2.8%, mean ± SD), or only during the autotrophic period (14.0 ± 0.0%). Haemal lordosis development, the second most severe skeletal deformity, presented fluctuating response against water temperature up to 14-16. mm TL (3.0 ± 4.2 to 13.0 ± 9.9%). However, the application of 22°C during the juvenile period resulted in the lowest and less variable incidence of haemal lordosis (1.0 ± 0.0 to 5.0 ± 1.4%). The mild deformities of caudal and dorsal fins presented different responses to water temperature. The prevalence of caudal-fin deformities was elevated when 16°C temperature was applied during the exotrophic larval period (54.0 ± 8.5%), while dorsal-fin deformities were favoured when 22°C temperature was applied during the autotrophic and exotrophic phases (35.0 ± 9.9 to 39.0 ± 4.2%). In the examined thermal range, growth rate was significantly elevated with the temperature increase. Fish survival was higher in the treatments where temperature after yolk-consumption increased from 16 or 19°C to 19 or 22°C, respectively. The results are discussed in respect to the onset of ontogeny of the different skeletal elements and the need of applying different thermal conditions during the development of S. aurata. © 2010 Elsevier B.V. |
2009 |
Koumoundouros, G; Ashton, C; Sfakianakis, D G; Divanach, P; Kentouri, M; Anthwal, N; Stickland, N C Thermally induced phenotypic plasticity of swimming performance in European sea bass Dicentrarchus labrax juveniles Journal Article Journal of Fish Biology, 74 (6), pp. 1309–1322, 2009, ISSN: 00221112, 10958649. @article{koumoundouros_thermally_2009, title = {Thermally induced phenotypic plasticity of swimming performance in European sea bass Dicentrarchus labrax juveniles}, author = {G Koumoundouros and C Ashton and D G Sfakianakis and P Divanach and M Kentouri and N Anthwal and N C Stickland}, url = {http://doi.wiley.com/10.1111/j.1095-8649.2009.02206.x}, doi = {10.1111/j.1095-8649.2009.02206.x}, issn = {00221112, 10958649}, year = {2009}, date = {2009-04-01}, urldate = {2020-08-18}, journal = {Journal of Fish Biology}, volume = {74}, number = {6}, pages = {1309--1322}, abstract = {The vulnerability of embryonic and larval stages of European sea bass Dicentrarchus labrax to environmental temperature and the longer-term consequences for the early juveniles was demonstrated. This phenotypic plasticity was highlighted by subjecting D. labrax at 15·2 ± 0·3 or 20·0 ± 0·4° C (mean ± s.d.) up to metamorphosis and then at the same temperature (18·5 ± 0·7° C). After 4-6 weeks at the same temperature, the measurement of critical swimming speed at four exercise temperatures (15, 20, 25 and 28° C) showed a significantly higher swimming capacity in the fish initially reared at 15° C than for fish initially reared at 20° C. This performance was correlated with significant differences in the phenotype of red muscle. Thermally induced phenotypic plasticity was clearly demonstrated as an important mechanism controlling swimming performance in early juveniles of D. labrax. © 2009 The Fisheries Society of the British Isles.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The vulnerability of embryonic and larval stages of European sea bass Dicentrarchus labrax to environmental temperature and the longer-term consequences for the early juveniles was demonstrated. This phenotypic plasticity was highlighted by subjecting D. labrax at 15·2 ± 0·3 or 20·0 ± 0·4° C (mean ± s.d.) up to metamorphosis and then at the same temperature (18·5 ± 0·7° C). After 4-6 weeks at the same temperature, the measurement of critical swimming speed at four exercise temperatures (15, 20, 25 and 28° C) showed a significantly higher swimming capacity in the fish initially reared at 15° C than for fish initially reared at 20° C. This performance was correlated with significant differences in the phenotype of red muscle. Thermally induced phenotypic plasticity was clearly demonstrated as an important mechanism controlling swimming performance in early juveniles of D. labrax. © 2009 The Fisheries Society of the British Isles. |
Chatzifotis, S; Arias, M V; Papadakis, I E; Divanach, P Evaluation of feed stimulants in diets for sea bream (Sparus aurata) Journal Article Israeli Journal of Aquaculture - Bamidgeh, 61 (4), pp. 315–321, 2009, ISSN: 0792156X. @article{chatzifotis_evaluation_2009, title = {Evaluation of feed stimulants in diets for sea bream (Sparus aurata)}, author = {S Chatzifotis and M V Arias and I E Papadakis and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-75649099386&partnerID=40&md5=bc0d7bc111cad49326e8f8416ddf1524}, issn = {0792156X}, year = {2009}, date = {2009-01-01}, journal = {Israeli Journal of Aquaculture - Bamidgeh}, volume = {61}, number = {4}, pages = {315--321}, abstract = {Six isoprotein and isolipidic diets were formulated to investigate the effect of dietary additives on growth and feed efficiency of sea bream (Sparus aurata) fry fed a fishmeal-based diet for 80 days. The additives (protorsan, hydrolyzed fish protein, squid meal, krill meal, and betaine + inosine-5'- monophosphate) were added to the diets at the expense of fishmeal. The specific growth rates of the fish ranged from 2.17-2.18% per day for the protorsan and control groups to 2.42% per day for the group fed the squid additive. Significant differences (ptextless0.05) in final body weight and specific growth rate were detected only between the protorsan and control groups and the squid additive group. The feed conversion ratio ranged from 1.04 in the group fed hydrolyzed fish protein group to 1.24 in the group fed krill with no statistically significant differences (ptextgreater0.05) between treatments. The feed stimulating action of taurine was tested by observation. Pellets coated in a taurine solution were more actively consumed than control pellets during the morning feeding but consumption did not differ during the afternoon feeding.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Six isoprotein and isolipidic diets were formulated to investigate the effect of dietary additives on growth and feed efficiency of sea bream (Sparus aurata) fry fed a fishmeal-based diet for 80 days. The additives (protorsan, hydrolyzed fish protein, squid meal, krill meal, and betaine + inosine-5'- monophosphate) were added to the diets at the expense of fishmeal. The specific growth rates of the fish ranged from 2.17-2.18% per day for the protorsan and control groups to 2.42% per day for the group fed the squid additive. Significant differences (ptextless0.05) in final body weight and specific growth rate were detected only between the protorsan and control groups and the squid additive group. The feed conversion ratio ranged from 1.04 in the group fed hydrolyzed fish protein group to 1.24 in the group fed krill with no statistically significant differences (ptextgreater0.05) between treatments. The feed stimulating action of taurine was tested by observation. Pellets coated in a taurine solution were more actively consumed than control pellets during the morning feeding but consumption did not differ during the afternoon feeding. |
Papadakis, I E; Zaiss, M M; Kyriakou, Y; Georgiou, G; Divanach, P; Mylonas, C C Aquaculture, 286 (1-2), pp. 45–52, 2009, ISSN: 00448486. @article{papadakis_histological_2009, title = {Histological evaluation of the elimination of Artemia nauplii from larval rearing protocols on the digestive system ontogeny of shi drum (Umbrina cirrosa L.)}, author = {I E Papadakis and M M Zaiss and Y Kyriakou and G Georgiou and P Divanach and C C Mylonas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-56649095808&doi=10.1016%2fj.aquaculture.2008.08.028&partnerID=40&md5=98c4d0d5f556cfa435dead4371ff509a}, doi = {10.1016/j.aquaculture.2008.08.028}, issn = {00448486}, year = {2009}, date = {2009-01-01}, journal = {Aquaculture}, volume = {286}, number = {1-2}, pages = {45--52}, abstract = {The influence of the absence of Artemia nauplii from larval diet protocols on growth and digestive system ontogeny was studied using histological techniques in the shi drum (Umbrina cirrosa). One group of larvae was reared using the standard intensive rearing protocol, which offers a combination of enriched rotifers (Brachionus plicatilis), Artemia spp. nauplii and artificial diet (Std-group). Another group was reared using the same protocol, but without the offering of Artemia nauplii (group No-Artemia). The ontogenesis of the digestive system from hatching to metamorphosis was a very rapid process, and there were no differences between the two feeding regimes in the temporal appearance of the various components of the digestive system. The first organised presence of the hepatic and pancreatic tissue appeared at 2-3 d after hatching (dah), suggesting that these organs function from a very early developmental stage. In the No-Artemia larvae between 13 and 29 dah there was a reduction in the height of enterocytes in the intestinal mucosa, a progressive flattening of the primary intestinal folds in the anterior and posterior intestine and a decrease in lipid stores in the liver, suggesting a period of relative starvation. However, by the end of the study at 41 dah, there were no significant differences in body length, intestinal morphology or liver lipid stores between larvae reared under the two feeding regimes. The study suggests that the diet may influence the maturation and/or function, but not the ontogeny of the digestive system. Furthermore, the rapid differentiation of the digestive system in shi drum and the prompt recovery of the No-Artemia larvae from the symptoms of starvation by 29 dah, indicate a plasticity during ontogenesis and the ability of larvae to adapt to artificial diets at very early developmental stages. © 2008 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The influence of the absence of Artemia nauplii from larval diet protocols on growth and digestive system ontogeny was studied using histological techniques in the shi drum (Umbrina cirrosa). One group of larvae was reared using the standard intensive rearing protocol, which offers a combination of enriched rotifers (Brachionus plicatilis), Artemia spp. nauplii and artificial diet (Std-group). Another group was reared using the same protocol, but without the offering of Artemia nauplii (group No-Artemia). The ontogenesis of the digestive system from hatching to metamorphosis was a very rapid process, and there were no differences between the two feeding regimes in the temporal appearance of the various components of the digestive system. The first organised presence of the hepatic and pancreatic tissue appeared at 2-3 d after hatching (dah), suggesting that these organs function from a very early developmental stage. In the No-Artemia larvae between 13 and 29 dah there was a reduction in the height of enterocytes in the intestinal mucosa, a progressive flattening of the primary intestinal folds in the anterior and posterior intestine and a decrease in lipid stores in the liver, suggesting a period of relative starvation. However, by the end of the study at 41 dah, there were no significant differences in body length, intestinal morphology or liver lipid stores between larvae reared under the two feeding regimes. The study suggests that the diet may influence the maturation and/or function, but not the ontogeny of the digestive system. Furthermore, the rapid differentiation of the digestive system in shi drum and the prompt recovery of the No-Artemia larvae from the symptoms of starvation by 29 dah, indicate a plasticity during ontogenesis and the ability of larvae to adapt to artificial diets at very early developmental stages. © 2008 Elsevier B.V. All rights reserved. |
Katharios, P; Divanach, P; Georgakopoulou, E; Koumoundouros, G Temperature limits for gilthead seabream and seabass Incollection FEAP, (Ed.): Control of malformation in fish aquaculture: Science and practice, pp. 43–45, Federation of European Aquaculture Producers, Belgium, 2009. @incollection{katharios_temperature_2009, title = {Temperature limits for gilthead seabream and seabass}, author = {P Katharios and P Divanach and E Georgakopoulou and G Koumoundouros}, editor = {FEAP}, year = {2009}, date = {2009-01-01}, booktitle = {Control of malformation in fish aquaculture: Science and practice}, pages = {43--45}, publisher = {Federation of European Aquaculture Producers}, address = {Belgium}, keywords = {}, pubstate = {published}, tppubtype = {incollection} } |
Suquet, M; Divanach, P; Hussenot, J; Coves, D; Fauvel, C Marine fish culture of "new species" farmed in Europe Journal Article Agricultures, 18 , pp. 148 – 156, 2009, (Original title (in French): Pisciculture marine de "nouvelles espéces" d’élevage pour l’EuropeCahiers). @article{suquet_marine_2009, title = {Marine fish culture of "new species" farmed in Europe}, author = {M Suquet and P Divanach and J Hussenot and D Coves and C Fauvel}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-70249124237&partnerID=40&md5=ed687af413b8a94c76e68cf9e43aee57}, year = {2009}, date = {2009-01-01}, journal = {Agricultures}, volume = {18}, pages = {148 -- 156}, abstract = {In Europe, fish aquaculture in a marine environment developed following two successive waves: Atlantic salmon and then sea-bass and sea-bream. Rearing "new marine fish species" is sustained for many complementary reasons. This paper aims at presenting the aquaculture status of these "new species," research carried out and then problems and perspectives suggested by farming development in these species. © 2007 John Libbey Eurotext - All rights reserved.}, note = {Original title (in French): Pisciculture marine de "nouvelles espéces" d’élevage pour l’EuropeCahiers}, keywords = {}, pubstate = {published}, tppubtype = {article} } In Europe, fish aquaculture in a marine environment developed following two successive waves: Atlantic salmon and then sea-bass and sea-bream. Rearing "new marine fish species" is sustained for many complementary reasons. This paper aims at presenting the aquaculture status of these "new species," research carried out and then problems and perspectives suggested by farming development in these species. © 2007 John Libbey Eurotext - All rights reserved. |
2008 |
Papazi, Aikaterini; Makridis, Pavlos; Divanach, Pascal; Kotzabasis, Kiriakos Physiologia Plantarum, 132 (3), pp. 338–349, 2008, ISSN: 0031-9317, 1399-3054. @article{papazi_bioenergetic_2008, title = {Bioenergetic changes in the microalgal photosynthetic apparatus by extremely high CO $_textrm2$ concentrations induce an intense biomass production}, author = {Aikaterini Papazi and Pavlos Makridis and Pascal Divanach and Kiriakos Kotzabasis}, url = {http://doi.wiley.com/10.1111/j.1399-3054.2007.01015.x}, doi = {10.1111/j.1399-3054.2007.01015.x}, issn = {0031-9317, 1399-3054}, year = {2008}, date = {2008-03-01}, urldate = {2020-08-17}, journal = {Physiologia Plantarum}, volume = {132}, number = {3}, pages = {338--349}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Chatzifotis, Stavros; Polemitou, Irene; Divanach, Pascal; Antonopoulou, Efthimia Aquaculture, 275 (1-4), pp. 201–208, 2008, ISSN: 00448486. @article{chatzifotis_effect_2008, title = {Effect of dietary taurine supplementation on growth performance and bile salt activated lipase activity of common dentex, Dentex dentex, fed a fish meal/soy protein concentrate-based diet}, author = {Stavros Chatzifotis and Irene Polemitou and Pascal Divanach and Efthimia Antonopoulou}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848607011672}, doi = {10.1016/j.aquaculture.2007.12.013}, issn = {00448486}, year = {2008}, date = {2008-03-01}, urldate = {2020-08-31}, journal = {Aquaculture}, volume = {275}, number = {1-4}, pages = {201--208}, abstract = {Common dentex were fed three isoprotein and isoenergetic diets to investigate the effect of partial substitution of fish meal by soy protein concentrate (0 [FM], 25 [SM25] and 40% [SM40]) in the presence or absence of taurine (T) supplementation (2 g kg- 1 on a dry weight basis) [FM + T], [SM25 + T] and [SM40 + T], on growth related parameters, muscle composition and bile salt activated lipase activity. The fish were fed twice a day by hand to apparent satiation over the course of a 12-week growth trial. The [SM40] diet resulted in lower feed intake compared to the [FM] and [SM25] diets, lower specific growth rate compared to the [FM] diet, lower condition factor and increased mortality of dentex compared to the [FM] and [SM25] diets. On the other hand, the [SM25] diet had no adverse effect on either feed intake or specific growth rate of dentex compared to the [FM] diet. The hepatosomatic and mesenteric fat indices did not change by increasing soy content. Moreover, no difference was observed in the composition of muscle in dentex fed different proportions of soy protein concentrate. However, the activity of the bile salt-activated lipase in liver was significantly lower in fish fed the [SM25] diet than in fish fed the [FM] diet. Dietary taurine supplementation increased the specific growth rate and daily feed intake while it reduced feed conversion ratio of dentex. Comparison among groups showed that dietary taurine supplementation increased feed intake but not specific growth rate in the [SM40 + T] group compared to [SM40] group. The condition factor was higher in fish fed the [FM] diet compared to the [SM25 + T] and [SM40 + T] diets. However, no difference was observed between the [SM25] and [SM40] groups compared with the [SM25 + T] and [SM40 + T] groups, respectively. Analysis of the chemical composition of the muscle showed that the [SM25 + T] fed fish had higher crude protein compared to the [SM40 + T] fed fish. Furthermore, the mesenteric lipid index was higher in [SM25 + T] fed fish compared to those fed the [SM25] and [SM40 + T] diets. The activity of the bile salt-activated lipase in the liver increased in fish fed the three diets [FM], [SM25] and [SM40] in the presence of taurine, indicating that taurine in diets at 2 g kg- 1 may improve lipid metabolism, although no significant differences were observed in the total fat content of the muscle of fish fed either of these experimental diets. In conclusion, it appears that in dentex, partial substitution of fish meal is possible with 25% of soy protein concentrate without affecting growth, which can be improved further by dietary taurine supplementation of 2 g kg- 1 diet. © 2007 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Common dentex were fed three isoprotein and isoenergetic diets to investigate the effect of partial substitution of fish meal by soy protein concentrate (0 [FM], 25 [SM25] and 40% [SM40]) in the presence or absence of taurine (T) supplementation (2 g kg- 1 on a dry weight basis) [FM + T], [SM25 + T] and [SM40 + T], on growth related parameters, muscle composition and bile salt activated lipase activity. The fish were fed twice a day by hand to apparent satiation over the course of a 12-week growth trial. The [SM40] diet resulted in lower feed intake compared to the [FM] and [SM25] diets, lower specific growth rate compared to the [FM] diet, lower condition factor and increased mortality of dentex compared to the [FM] and [SM25] diets. On the other hand, the [SM25] diet had no adverse effect on either feed intake or specific growth rate of dentex compared to the [FM] diet. The hepatosomatic and mesenteric fat indices did not change by increasing soy content. Moreover, no difference was observed in the composition of muscle in dentex fed different proportions of soy protein concentrate. However, the activity of the bile salt-activated lipase in liver was significantly lower in fish fed the [SM25] diet than in fish fed the [FM] diet. Dietary taurine supplementation increased the specific growth rate and daily feed intake while it reduced feed conversion ratio of dentex. Comparison among groups showed that dietary taurine supplementation increased feed intake but not specific growth rate in the [SM40 + T] group compared to [SM40] group. The condition factor was higher in fish fed the [FM] diet compared to the [SM25 + T] and [SM40 + T] diets. However, no difference was observed between the [SM25] and [SM40] groups compared with the [SM25 + T] and [SM40 + T] groups, respectively. Analysis of the chemical composition of the muscle showed that the [SM25 + T] fed fish had higher crude protein compared to the [SM40 + T] fed fish. Furthermore, the mesenteric lipid index was higher in [SM25 + T] fed fish compared to those fed the [SM25] and [SM40 + T] diets. The activity of the bile salt-activated lipase in the liver increased in fish fed the three diets [FM], [SM25] and [SM40] in the presence of taurine, indicating that taurine in diets at 2 g kg- 1 may improve lipid metabolism, although no significant differences were observed in the total fat content of the muscle of fish fed either of these experimental diets. In conclusion, it appears that in dentex, partial substitution of fish meal is possible with 25% of soy protein concentrate without affecting growth, which can be improved further by dietary taurine supplementation of 2 g kg- 1 diet. © 2007 Elsevier B.V. All rights reserved. |
Chatzifotis, S; Kokou, F; Ampatzis, K; Papadakis, I E; Divanach, P; Dermon, C R Aquaculture Nutrition, 14 (5), pp. 405–415, 2008, ISSN: 13535773. @article{chatzifotis_effects_2008, title = {Effects of dietary caffeine on growth, body composition, somatic indexes, and cerebral distribution of acetyl-cholinesterase and nitric oxide synthase in gilthead sea bream (Sparus aurata), reared in winter temperature}, author = {S Chatzifotis and F Kokou and K Ampatzis and I E Papadakis and P Divanach and C R Dermon}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-51349164015&doi=10.1111%2fj.1365-2095.2007.00541.x&partnerID=40&md5=5b17b1a62bdf3d88373fd728afada0b0}, doi = {10.1111/j.1365-2095.2007.00541.x}, issn = {13535773}, year = {2008}, date = {2008-01-01}, journal = {Aquaculture Nutrition}, volume = {14}, number = {5}, pages = {405--415}, abstract = {This study aims at examining the effect of caffeine administration on growth, feed efficiency, and consumption of sea bream (Sparus aurata), reared in winter temperatures. Moreover, it is questioned whether caffeine has a central action in the brain and its effects are partly mediated via central brain mechanisms. For this, we studied the influences of caffeine treatment on the cerebral pattern of the cholinergic neurotransmission and the novel neuromodulator nitric oxide (NO), by means of acetyl-cholinesterase (AchE) and nitric oxide synthase (NOS) histochemistry. Five different diets containing 0.0, 0.1, 1.0, 2.0 and 5.0 g caffeine kg-1 of diet were administrated to five groups of fish. Caffeine adversely affected sea-bream growth at a concentration higher than 1 g kg-1 diet and increased feed conversion ratio in the treatments of 2 and 5 g kg-1 (P < 0.05). The daily consumption of feeds was similar to all groups, indicating that caffeine did not influence diet palatability. AChE- and NADPH-diaphorase histochemistry showed densely labeled cells and fibers mainly in dorsal telencephalon, preoptic, pretectal, hypothalamic areas, optic tectum, reticular formation, cerebellum and motor nuclei. When compared with matched caffeine-treated animals, no differences in the histochemical pattern and cell densities of cerebral AChE and NADPH-diaphorase were found. © 2008 Blackwell Publishing Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study aims at examining the effect of caffeine administration on growth, feed efficiency, and consumption of sea bream (Sparus aurata), reared in winter temperatures. Moreover, it is questioned whether caffeine has a central action in the brain and its effects are partly mediated via central brain mechanisms. For this, we studied the influences of caffeine treatment on the cerebral pattern of the cholinergic neurotransmission and the novel neuromodulator nitric oxide (NO), by means of acetyl-cholinesterase (AchE) and nitric oxide synthase (NOS) histochemistry. Five different diets containing 0.0, 0.1, 1.0, 2.0 and 5.0 g caffeine kg-1 of diet were administrated to five groups of fish. Caffeine adversely affected sea-bream growth at a concentration higher than 1 g kg-1 diet and increased feed conversion ratio in the treatments of 2 and 5 g kg-1 (P < 0.05). The daily consumption of feeds was similar to all groups, indicating that caffeine did not influence diet palatability. AChE- and NADPH-diaphorase histochemistry showed densely labeled cells and fibers mainly in dorsal telencephalon, preoptic, pretectal, hypothalamic areas, optic tectum, reticular formation, cerebellum and motor nuclei. When compared with matched caffeine-treated animals, no differences in the histochemical pattern and cell densities of cerebral AChE and NADPH-diaphorase were found. © 2008 Blackwell Publishing Ltd. |
Papadakis, I E; Chatzifotis, S; Divanach, P; Kentouri, M Weaning of greater amberjack (Seriola dumerilii Risso 1810) juveniles from moist to dry pellet Journal Article Aquaculture International, 16 (1), pp. 13–25, 2008, ISSN: 09676120. @article{papadakis_weaning_2008, title = {Weaning of greater amberjack (Seriola dumerilii Risso 1810) juveniles from moist to dry pellet}, author = {I E Papadakis and S Chatzifotis and P Divanach and M Kentouri}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-38149134529&doi=10.1007%2fs10499-007-9118-x&partnerID=40&md5=7a53d9f9573132c2ddb1145ec1d41d32}, doi = {10.1007/s10499-007-9118-x}, issn = {09676120}, year = {2008}, date = {2008-01-01}, journal = {Aquaculture International}, volume = {16}, number = {1}, pages = {13--25}, abstract = {The weaning of greater amberjack (Seriola dumerilii Risso 1810) to a dry diet was studied in two consecutive experiments. The experimental fish were hatchery-produced and grown to their initial size on a soft pellet containing 50% trash fish and 50% commercial dry pellet. In the first experiment, three homogenous groups of greater amberjacks (n = 153, mean weight: 374 ± 43 g), initially adapted for a period of 30 days to a diet with a 40% moisture content, were fed on commercial pellets containing three different levels of dietary moisture (7, 20 and 40%, respectively) for 126 days. We found that fish needed a month to adapt to the 7%- and 20%-moisture diets and that moisture content of the diet had a significant effect on fish growth in terms of increasing feed consumption and lowering feed conversion ratio. The group fed on the 20%-moisture diet not only compensated for the initial adaptation period but presented the higher growth rate and best feed conversion ratio. In the second experiment, all groups of experiment 1 were fed the same commercial diet with a 7% moisture content for 72 days. The group that had been previously fed on the 20%-moisture diet showed the best growth performance. The implication of the above results for the weaning of greater amberjack to dry diets is discussed in terms of the digestion process and physiology. © Springer Science+Business Media B.V. 2007.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The weaning of greater amberjack (Seriola dumerilii Risso 1810) to a dry diet was studied in two consecutive experiments. The experimental fish were hatchery-produced and grown to their initial size on a soft pellet containing 50% trash fish and 50% commercial dry pellet. In the first experiment, three homogenous groups of greater amberjacks (n = 153, mean weight: 374 ± 43 g), initially adapted for a period of 30 days to a diet with a 40% moisture content, were fed on commercial pellets containing three different levels of dietary moisture (7, 20 and 40%, respectively) for 126 days. We found that fish needed a month to adapt to the 7%- and 20%-moisture diets and that moisture content of the diet had a significant effect on fish growth in terms of increasing feed consumption and lowering feed conversion ratio. The group fed on the 20%-moisture diet not only compensated for the initial adaptation period but presented the higher growth rate and best feed conversion ratio. In the second experiment, all groups of experiment 1 were fed the same commercial diet with a 7% moisture content for 72 days. The group that had been previously fed on the 20%-moisture diet showed the best growth performance. The implication of the above results for the weaning of greater amberjack to dry diets is discussed in terms of the digestion process and physiology. © Springer Science+Business Media B.V. 2007. |
Katharios, P; Papadaki, M; Papandroulakis, N; Divanach, P Severe mortality in mesocosm-reared sharpsnout sea bream Diplodus puntazzo larvae due to epitheliocystis infection Journal Article Diseases of Aquatic Organisms, 82 (1), pp. 55–60, 2008, ISSN: 01775103. @article{katharios_severe_2008, title = {Severe mortality in mesocosm-reared sharpsnout sea bream Diplodus puntazzo larvae due to epitheliocystis infection}, author = {P Katharios and M Papadaki and N Papandroulakis and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-56649123097&doi=10.3354%2fdao01968&partnerID=40&md5=1c652a653ce36b0e7ae3f6a7b9d1aff6}, doi = {10.3354/dao01968}, issn = {01775103}, year = {2008}, date = {2008-01-01}, journal = {Diseases of Aquatic Organisms}, volume = {82}, number = {1}, pages = {55--60}, abstract = {This paper describes severe mortalities recorded in sharpsnout sea bream Diplodus puntazzo larvae reared in mesocosms. The mortalities were attributed to epitheliocystis infection. The pathology associated with the disease is described using histological techniques. Microscopical examination showed a massive infection of the skin, fins, and oral cavity, with impaired feeding, respiration, and osmoregulation being the most likely cause of death. This is the first report of epitheliocystis disease in sharpsnout sea bream and in fish at such an early developmental stage. © Inter-Research 2008.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This paper describes severe mortalities recorded in sharpsnout sea bream Diplodus puntazzo larvae reared in mesocosms. The mortalities were attributed to epitheliocystis infection. The pathology associated with the disease is described using histological techniques. Microscopical examination showed a massive infection of the skin, fins, and oral cavity, with impaired feeding, respiration, and osmoregulation being the most likely cause of death. This is the first report of epitheliocystis disease in sharpsnout sea bream and in fish at such an early developmental stage. © Inter-Research 2008. |
Papandroulakis, N; Mylonas, C C; Syggelaki, E; Katharios, P; Divanach, P First reproduction of captive-reared wreckfish (Polyprion americanus) using GnRH implants. Inproceedings Aquaculture Europe 08, European Aquaculture Society, Krakow, Poland, 2008, (Backup Publisher: European Aquaculture Society). @inproceedings{papandroulakis_first_2008, title = {First reproduction of captive-reared wreckfish (Polyprion americanus) using GnRH implants.}, author = {N Papandroulakis and C C Mylonas and E Syggelaki and P Katharios and P Divanach}, year = {2008}, date = {2008-01-01}, booktitle = {Aquaculture Europe 08}, publisher = {European Aquaculture Society}, address = {Krakow, Poland}, note = {Backup Publisher: European Aquaculture Society}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Katharios, P; Papandroulakis, N; Papadaki, M; Syggelaki, E; Tzamarias, D; Gounalaki, N; Divanach, P Pseudomonas putida infection in captive wreckfish Polyprion americanus. A case report. Inproceedings Aquaculture Europe 08, pp. 307, European Aquacultrure Society, Krakow, Poland, 2008, (Backup Publisher: European Aquacultrure Society). @inproceedings{katharios_pseudomonas_2008, title = {Pseudomonas putida infection in captive wreckfish Polyprion americanus. A case report.}, author = {P Katharios and N Papandroulakis and M Papadaki and E Syggelaki and D Tzamarias and N Gounalaki and P Divanach}, year = {2008}, date = {2008-01-01}, booktitle = {Aquaculture Europe 08}, pages = {307}, publisher = {European Aquacultrure Society}, address = {Krakow, Poland}, note = {Backup Publisher: European Aquacultrure Society}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
2007 |
Fanouraki, E; Divanach, P; Pavlidis, M Baseline values for acute and chronic stress indicators in sexually immature red porgy (Pagrus pagrus) Journal Article Aquaculture, 265 (1-4), pp. 294–304, 2007, ISSN: 00448486. @article{fanouraki_baseline_2007, title = {Baseline values for acute and chronic stress indicators in sexually immature red porgy (Pagrus pagrus)}, author = {E Fanouraki and P Divanach and M Pavlidis}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848607000397}, doi = {10.1016/j.aquaculture.2007.01.006}, issn = {00448486}, year = {2007}, date = {2007-05-01}, urldate = {2020-08-17}, journal = {Aquaculture}, volume = {265}, number = {1-4}, pages = {294--304}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Georgakopoulou, E; Angelopoulou, A; Kaspiris, P; Divanach, P; Koumoundouros, G Temperature effects on cranial deformities in European sea bass, Dicentrarchus labrax (L.) Journal Article Journal of Applied Ichthyology, 23 (1), pp. 99–103, 2007, ISSN: 0175-8659, 1439-0426. @article{georgakopoulou_temperature_2007, title = {Temperature effects on cranial deformities in European sea bass, Dicentrarchus labrax (L.)}, author = {E Georgakopoulou and A Angelopoulou and P Kaspiris and P Divanach and G Koumoundouros}, url = {http://doi.wiley.com/10.1111/j.1439-0426.2006.00810.x}, doi = {10.1111/j.1439-0426.2006.00810.x}, issn = {0175-8659, 1439-0426}, year = {2007}, date = {2007-02-01}, urldate = {2020-08-18}, journal = {Journal of Applied Ichthyology}, volume = {23}, number = {1}, pages = {99--103}, abstract = {The present study investigates the effect of water temperature on the development of deformities during embryonic and larval stages of European sea bass, Dicentrarchus labrax (L.). Two temperature conditions were examined in duplicate by studying the presence of skeletal deformities in a sample of 45 to 51 fish taken from each population at the end of the rearing trials [20-23 mm total length (TL)]. The results indicated that water temperature during the embryonic and larval phase has significant effects on deformation of the branchiostegal rays (P textless 0.001), but not of the mouth and the fins (P textgreater 0.05). At 15°C, 27.2-33.4% of the examined fish had branchiostegal rays of abnormal shape and/or orientation, whereas at 20°C this deformity had a frequency of only 4.0-4.1%. The frequency distribution graph of branchiostegal counts demonstrated a significant deviation in the deformed fish from the normal (seven rays on each side of the body) phenotype at both temperatures tested. This deviation was mainly expressed as a lack of one to four rays (56.7% of deformed fish), or the formation of one extra ray (26.7% of deformed fish) (P textless 0.001, G-test). The results are discussed in respect to the possible mechanisms of temperature effects on the development of skeletal deformities. © 2007 The Authors Journal compilation © 2007 Blackwell Verlag, Berlin.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The present study investigates the effect of water temperature on the development of deformities during embryonic and larval stages of European sea bass, Dicentrarchus labrax (L.). Two temperature conditions were examined in duplicate by studying the presence of skeletal deformities in a sample of 45 to 51 fish taken from each population at the end of the rearing trials [20-23 mm total length (TL)]. The results indicated that water temperature during the embryonic and larval phase has significant effects on deformation of the branchiostegal rays (P textless 0.001), but not of the mouth and the fins (P textgreater 0.05). At 15°C, 27.2-33.4% of the examined fish had branchiostegal rays of abnormal shape and/or orientation, whereas at 20°C this deformity had a frequency of only 4.0-4.1%. The frequency distribution graph of branchiostegal counts demonstrated a significant deviation in the deformed fish from the normal (seven rays on each side of the body) phenotype at both temperatures tested. This deviation was mainly expressed as a lack of one to four rays (56.7% of deformed fish), or the formation of one extra ray (26.7% of deformed fish) (P textless 0.001, G-test). The results are discussed in respect to the possible mechanisms of temperature effects on the development of skeletal deformities. © 2007 The Authors Journal compilation © 2007 Blackwell Verlag, Berlin. |
Pavlidis, M; Futter, W C; Katharios, P; Divanach, P Blood cell profile of six Mediterranean mariculture fish species Journal Article Journal of Applied Ichthyology, 23 (1), pp. 70–73, 2007, ISSN: 01758659. @article{pavlidis_blood_2007, title = {Blood cell profile of six Mediterranean mariculture fish species}, author = {M Pavlidis and W C Futter and P Katharios and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33846307101&doi=10.1111%2fj.1439-0426.2006.00771.x&partnerID=40&md5=e1768163fb5c96efc554987a9d488e39}, doi = {10.1111/j.1439-0426.2006.00771.x}, issn = {01758659}, year = {2007}, date = {2007-01-01}, journal = {Journal of Applied Ichthyology}, volume = {23}, number = {1}, pages = {70--73}, abstract = {The haematological profile and a description of the cell types from the peripheral blood of six Mediterranean fish species are presented. The highest haematocrit value was recorded in the saupe, Sarpa salpa (P textless 0.001), the only herbivorous species, and which also lacked monocyte cells. Eosinophils were absent from the blood of the European sea bass Dicentrarchus labrax. White sea bream, Diplodus sargus and gilthead sea bream, Sparus aurata had statistically significantly high numbers of neutrophils and low numbers of lymphocytes (P textless 0.001). The numbers of different leucocyte cell types were not influenced by sex or maturity stage in any species, although some variation in the maximum diameter of the cells was observed. © 2007 The Authors Journal compilation © 2007 Blackwell Verlag, Berlin.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The haematological profile and a description of the cell types from the peripheral blood of six Mediterranean fish species are presented. The highest haematocrit value was recorded in the saupe, Sarpa salpa (P textless 0.001), the only herbivorous species, and which also lacked monocyte cells. Eosinophils were absent from the blood of the European sea bass Dicentrarchus labrax. White sea bream, Diplodus sargus and gilthead sea bream, Sparus aurata had statistically significantly high numbers of neutrophils and low numbers of lymphocytes (P textless 0.001). The numbers of different leucocyte cell types were not influenced by sex or maturity stage in any species, although some variation in the maximum diameter of the cells was observed. © 2007 The Authors Journal compilation © 2007 Blackwell Verlag, Berlin. |
Fanouraki, E; Laitinen, J T; Divanach, P; Pavlidis, M Endocrine regulation of skin blanching in red porgy, Pagrus pagrus Journal Article Annales Zoologici Fennici, 44 , pp. 241 – 248, 2007. @article{fanouraki_endocrine_2007, title = {Endocrine regulation of skin blanching in red porgy, Pagrus pagrus}, author = {E Fanouraki and J T Laitinen and P Divanach and M Pavlidis}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-34548814148&partnerID=40&md5=b66ebbdc2e41a3d8faf3e09be20cb2c1}, year = {2007}, date = {2007-01-01}, journal = {Annales Zoologici Fennici}, volume = {44}, pages = {241 -- 248}, abstract = {Previous research on red porgy, Pagrus pagrus, has shown that physiological colour change does not depend on changes in plasma cortisol or α-MSH. The purpose of this study was to identify the endocrine mechanism responsible for skin blanching in fish exposed to white background. There was no significant difference in serum cortisol, glucose, lactate, thyroid hormones, or plasma epinephrine concentration between black and white-background-adapted fish. Mean daily plasma melatonin concentration was significantly higher in black- (41.29 ± 2.46 pg ml-1) than white-background-exposed fish (32.07 ± 1.26 pg ml-1) while mean plasma norepinephrine was almost fivefold higher in white- (6.26 ± 1.57 ng ml-1) than in black-background-adapted fish (1.01 ± 0.34 ng ml-1). Skin melanin concentration did not differ significantly between the experimental groups. Our study indicates that skin blanching in red porgy is mediated through norepinephrine release. © Finnish Zoological and Botanical Publishing Board 2007.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Previous research on red porgy, Pagrus pagrus, has shown that physiological colour change does not depend on changes in plasma cortisol or α-MSH. The purpose of this study was to identify the endocrine mechanism responsible for skin blanching in fish exposed to white background. There was no significant difference in serum cortisol, glucose, lactate, thyroid hormones, or plasma epinephrine concentration between black and white-background-adapted fish. Mean daily plasma melatonin concentration was significantly higher in black- (41.29 ± 2.46 pg ml-1) than white-background-exposed fish (32.07 ± 1.26 pg ml-1) while mean plasma norepinephrine was almost fivefold higher in white- (6.26 ± 1.57 ng ml-1) than in black-background-adapted fish (1.01 ± 0.34 ng ml-1). Skin melanin concentration did not differ significantly between the experimental groups. Our study indicates that skin blanching in red porgy is mediated through norepinephrine release. © Finnish Zoological and Botanical Publishing Board 2007. |
Georgakopoulou, E; Sfakianakis, D G; Kouttouki, S; Divanach, P; Kentouri, M; Koumoundouros, G The influence of temperature during early life on phenotypic expression at later ontogenetic stages in sea bass Journal Article Journal of Fish Biology, 70 (1), pp. 278–291, 2007, ISSN: 0022-1112, 1095-8649. @article{georgakopoulou_influence_2007, title = {The influence of temperature during early life on phenotypic expression at later ontogenetic stages in sea bass}, author = {E Georgakopoulou and D G Sfakianakis and S Kouttouki and P Divanach and M Kentouri and G Koumoundouros}, url = {http://doi.wiley.com/10.1111/j.1095-8649.2007.01305.x}, doi = {10.1111/j.1095-8649.2007.01305.x}, issn = {0022-1112, 1095-8649}, year = {2007}, date = {2007-01-01}, urldate = {2020-08-18}, journal = {Journal of Fish Biology}, volume = {70}, number = {1}, pages = {278--291}, abstract = {To exam whether the temperature experienced by fishes at early developmental stages can influence their phenotype at subsequent stages, the model species used, European sea bass Dicentrarchus labrax was subjected to water temperatures of 15 or 20°C during the half-epiboly stage until the metamorphosis. Meristic and morphometric characters at three different stages, well after the end of the thermal treatments, were explored. Body shape and most of the meristic characters were significantly affected by the environmental temperature during their early life stages. Fish body shape at 15°C tended to be more slender than at 20°C. The dorsal spines and soft rays, the pectoral lepidotrichia and caudal dermatotrichia were significantly affected. Phenotypic differences due to the two thermal regimes are discussed in terms of their functional meaning during the transition from the planktonic to the littoral niche. © 2007 The Fisheries Society of the British Isles.}, keywords = {}, pubstate = {published}, tppubtype = {article} } To exam whether the temperature experienced by fishes at early developmental stages can influence their phenotype at subsequent stages, the model species used, European sea bass Dicentrarchus labrax was subjected to water temperatures of 15 or 20°C during the half-epiboly stage until the metamorphosis. Meristic and morphometric characters at three different stages, well after the end of the thermal treatments, were explored. Body shape and most of the meristic characters were significantly affected by the environmental temperature during their early life stages. Fish body shape at 15°C tended to be more slender than at 20°C. The dorsal spines and soft rays, the pectoral lepidotrichia and caudal dermatotrichia were significantly affected. Phenotypic differences due to the two thermal regimes are discussed in terms of their functional meaning during the transition from the planktonic to the littoral niche. © 2007 The Fisheries Society of the British Isles. |
2006 |
Katharios, P; Sterioti, A; Divanach, P Cretaquarium: Aquatic health related challenges in setting up a new public aquarium. Inproceedings Fifth International Symposium on Aquatic Animal Health, pp. 147, San Francisco, U.S., 2006. @inproceedings{katharios_cretaquarium_2006, title = {Cretaquarium: Aquatic health related challenges in setting up a new public aquarium.}, author = {P Katharios and A Sterioti and P Divanach}, year = {2006}, date = {2006-09-01}, booktitle = {Fifth International Symposium on Aquatic Animal Health}, pages = {147}, address = {San Francisco, U.S.}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
P., Katharios; N., Papandroulakis; C., Hayward; M., Asderis; Divanach, P Parasitic monogeneans in cultured fish in Crete. Methods for treatment. Inproceedings HCMR, (Ed.): 8th Congress of Oceanography and Fisheries, Thessaloniki, Greece, 2006. @inproceedings{katharios_parasitic_2006, title = {Parasitic monogeneans in cultured fish in Crete. Methods for treatment.}, author = {Katharios P. and Papandroulakis N. and Hayward C. and Asderis M. and P Divanach}, editor = {HCMR}, year = {2006}, date = {2006-06-01}, booktitle = {8th Congress of Oceanography and Fisheries}, address = {Thessaloniki, Greece}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Kouttouki, Savoula; Georgakopoulou, Eustathia; Kaspiris, Panagiotis; Divanach, Pascal; Koumoundouros, Georgios Shape ontogeny and variation in the sharpsnout seabream, Diplodus puntazzo (Cetti 1777) Journal Article Aquaculture Research, 37 (7), pp. 655–663, 2006, ISSN: 1355-557X, 1365-2109. @article{kouttouki_shape_2006, title = {Shape ontogeny and variation in the sharpsnout seabream, Diplodus puntazzo (Cetti 1777)}, author = {Savoula Kouttouki and Eustathia Georgakopoulou and Panagiotis Kaspiris and Pascal Divanach and Georgios Koumoundouros}, url = {http://doi.wiley.com/10.1111/j.1365-2109.2006.01475.x}, doi = {10.1111/j.1365-2109.2006.01475.x}, issn = {1355-557X, 1365-2109}, year = {2006}, date = {2006-05-01}, urldate = {2020-08-18}, journal = {Aquaculture Research}, volume = {37}, number = {7}, pages = {655--663}, abstract = {Shape ontogeny of Diplodus puntazzo was studied during the larval and metamorphosis phase (2.6-33.0 mm total length (TL)) by means of geometric morphometrics. Additionally, shape comparison was performed between newly settled wild individuals of D. puntazzo (11.0-18.0 mm TL) and reared of the same TL range. Results clearly demonstrated that shape ontogeny of D. puntazzo is not continuous during the studied period, but it is characterized by the presence of two inflection points (at 6.2 and 11.4 mm TL), which define three phases of significantly different rates of shape development (Ptextless0.05). Spline diagrams demonstrated that shape ontogeny mainly correlated with the development of fins, caudal peduncle, snoot and the ventral abdominal profile. Concerning the comparison of reared fish with wild fish, morphometric analysis revealed a significant effect of fish origin on the shape of D. puntazzo (Wilks’ λ=0.147, Ptextless0.001). Shape differences between the two populations were mainly demonstrated at the caudal peduncle and at the ventral profile of the abdominal area. Combined with the results of shape ontogeny, spline diagrams showed that shape differences between the two populations were related to the comparatively more advanced ontogenetic state of reared fish, at the studied size range. © 2006 Blackwell Publishing Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Shape ontogeny of Diplodus puntazzo was studied during the larval and metamorphosis phase (2.6-33.0 mm total length (TL)) by means of geometric morphometrics. Additionally, shape comparison was performed between newly settled wild individuals of D. puntazzo (11.0-18.0 mm TL) and reared of the same TL range. Results clearly demonstrated that shape ontogeny of D. puntazzo is not continuous during the studied period, but it is characterized by the presence of two inflection points (at 6.2 and 11.4 mm TL), which define three phases of significantly different rates of shape development (Ptextless0.05). Spline diagrams demonstrated that shape ontogeny mainly correlated with the development of fins, caudal peduncle, snoot and the ventral abdominal profile. Concerning the comparison of reared fish with wild fish, morphometric analysis revealed a significant effect of fish origin on the shape of D. puntazzo (Wilks’ λ=0.147, Ptextless0.001). Shape differences between the two populations were mainly demonstrated at the caudal peduncle and at the ventral profile of the abdominal area. Combined with the results of shape ontogeny, spline diagrams showed that shape differences between the two populations were related to the comparatively more advanced ontogenetic state of reared fish, at the studied size range. © 2006 Blackwell Publishing Ltd. |
Sfakianakis, D G; Georgakopoulou, E; Papadakis, I E; Divanach, P; Kentouri, M; Koumoundouros, G Environmental determinants of haemal lordosis in European sea bass, Dicentrarchus labrax (Linnaeus, 1758) Journal Article Aquaculture, 254 (1-4), pp. 54–64, 2006, ISSN: 00448486. @article{sfakianakis_environmental_2006, title = {Environmental determinants of haemal lordosis in European sea bass, Dicentrarchus labrax (Linnaeus, 1758)}, author = {D G Sfakianakis and E Georgakopoulou and I E Papadakis and P Divanach and M Kentouri and G Koumoundouros}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848605006332}, doi = {10.1016/j.aquaculture.2005.10.028}, issn = {00448486}, year = {2006}, date = {2006-04-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {254}, number = {1-4}, pages = {54--64}, abstract = {We tested the effect of water-temperature during the larval phase on sea bass sensitivity to current-induced lordosis. During larval rearing phase, fish were subjected to two different water temperatures (15 or 20 °C), with the rest of the rearing parameters remaining identical between the different populations. During the pregrowing phase, the different populations were subjected to four different water-current velocities (WCV), with the rest of the environmental conditions remaining common. At the end of the pregrowing phase (43.5 ± 0.8 mm TL), lordosis was more frequent in fish that developed at 20 °C (p textless 0.05), independently of the WCVs applied. The anatomic analysis of the deformed fish revealed a significant effect of early developmental temperature on the severity of the deformity, with the 15 °C populations showing the lower severity in respect to the angle of lordosis and the number of affected vertebrae (p textless 0.05). Two weeks of exposure to WCVs (24.0 ± 0.8 mm TL) resulted in 15 °C populations presenting the lower incidence of lordosis and the higher incidence of normal fish in all WCVs, except 50%, tested (p textless 0.05). At this stage, WCV had a clear effect on lordosis development in populations coming from both developmental temperatures (p textless 0.05), although at 15 °C these effects were more pronounced. Fish that developed at 20 °C showed significantly higher incidence of deformed vertebral centra and arches than those developed at 15 °C (p textless 0.05). These early anatomical signs were evident in samples taken at the end of the larval rearing phase (15.7 ± 1.0 mm TL), as well as 1 week after the onset of exposure to WCVs (21.4 ± 0.6 mm TL). In both cases, their incidence was significantly higher in fish that initially developed at 20 °C than at 15 °C. © 2005 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We tested the effect of water-temperature during the larval phase on sea bass sensitivity to current-induced lordosis. During larval rearing phase, fish were subjected to two different water temperatures (15 or 20 °C), with the rest of the rearing parameters remaining identical between the different populations. During the pregrowing phase, the different populations were subjected to four different water-current velocities (WCV), with the rest of the environmental conditions remaining common. At the end of the pregrowing phase (43.5 ± 0.8 mm TL), lordosis was more frequent in fish that developed at 20 °C (p textless 0.05), independently of the WCVs applied. The anatomic analysis of the deformed fish revealed a significant effect of early developmental temperature on the severity of the deformity, with the 15 °C populations showing the lower severity in respect to the angle of lordosis and the number of affected vertebrae (p textless 0.05). Two weeks of exposure to WCVs (24.0 ± 0.8 mm TL) resulted in 15 °C populations presenting the lower incidence of lordosis and the higher incidence of normal fish in all WCVs, except 50%, tested (p textless 0.05). At this stage, WCV had a clear effect on lordosis development in populations coming from both developmental temperatures (p textless 0.05), although at 15 °C these effects were more pronounced. Fish that developed at 20 °C showed significantly higher incidence of deformed vertebral centra and arches than those developed at 15 °C (p textless 0.05). These early anatomical signs were evident in samples taken at the end of the larval rearing phase (15.7 ± 1.0 mm TL), as well as 1 week after the onset of exposure to WCVs (21.4 ± 0.6 mm TL). In both cases, their incidence was significantly higher in fish that initially developed at 20 °C than at 15 °C. © 2005 Elsevier B.V. All rights reserved. |
Pavlidis, Michael; Kokokiris, Lambros; Paspatis, Michael; Somarakis, Stylianos; Kentouri, Maroudio; Divanach, Pascal Gonadal development in hybrids of Mediterranean sparids: Sparus aurata (female) xPagrus pagrus (male) Journal Article Aquaculture Research, 37 (3), pp. 302–305, 2006, ISSN: 1355-557X, 1365-2109. @article{pavlidis_gonadal_2006, title = {Gonadal development in hybrids of Mediterranean sparids: Sparus aurata (female) xPagrus pagrus (male)}, author = {Michael Pavlidis and Lambros Kokokiris and Michael Paspatis and Stylianos Somarakis and Maroudio Kentouri and Pascal Divanach}, url = {http://doi.wiley.com/10.1111/j.1365-2109.2005.01405.x}, doi = {10.1111/j.1365-2109.2005.01405.x}, issn = {1355-557X, 1365-2109}, year = {2006}, date = {2006-02-01}, urldate = {2020-08-18}, journal = {Aquaculture Research}, volume = {37}, number = {3}, pages = {302--305}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Chatzifotis, S; Martin-Prat, Villamor A; Limberis, N; Papandroulakis, N; Divanach, P First data on growth of cultured brown meagre Sciaena umbra using diets with different protein and fat contents Journal Article Fisheries Science, 72 (1), pp. 83–88, 2006, ISSN: 09199268. @article{chatzifotis_first_2006, title = {First data on growth of cultured brown meagre Sciaena umbra using diets with different protein and fat contents}, author = {S Chatzifotis and A Villamor Martin-Prat and N Limberis and N Papandroulakis and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33645073706&doi=10.1111%2fj.1444-2906.2006.01120.x&partnerID=40&md5=710f8cdfc624dfbc7ecd98730393dbf5}, doi = {10.1111/j.1444-2906.2006.01120.x}, issn = {09199268}, year = {2006}, date = {2006-01-01}, journal = {Fisheries Science}, volume = {72}, number = {1}, pages = {83--88}, abstract = {Brown meagre Sciaena umbra Linnaeus 1758 is a demersal species living at depths of 0-200 m with a wide distribution in the Mediterranean Sea, Black Sea and eastern Atlantic Ocean. It exhibits nocturnal behavior and occupies bottom caves and seabeds covered with vegetation. This study presents the first data on brown meagre growth under culture conditions and contributes some basic information on its dietary protein requirement by feeding isoenergetic diets with different protein-lipid ratios by means of self-feeders. Fish with 78.8 ± 15.8 g (mean ± standard deviation) body weight were divided into four groups and subjected to four different feeding regimes with three replications for 77 days. In the first group, fish were supplied with a high protein and low fat diet (HPLF, 52: 8 w/w fat-protein ratio), the second one a medium protein and medium fat diet (MPMF, 42: 14), the third with a low protein and high fat diet (LPHF, 31: 23). In the fourth group, fish had access to both HPLF and LPHF diets. Fish fed on HPLF and MPMF diets showed significantly better growth and feed conversion ratios than fish fed on the LPHF diet. Fish with access to HPLF and LPHF diets exhibited comparable growth to the HPLF group but their feed conversion ratio was significantly higher. The condition factor and hepatosomatic index revealed no significant differences between dietary regimes. The liver of brown meagre may serve as a depository organ for energy, judging from its relatively high lipid content (39-43%).}, keywords = {}, pubstate = {published}, tppubtype = {article} } Brown meagre Sciaena umbra Linnaeus 1758 is a demersal species living at depths of 0-200 m with a wide distribution in the Mediterranean Sea, Black Sea and eastern Atlantic Ocean. It exhibits nocturnal behavior and occupies bottom caves and seabeds covered with vegetation. This study presents the first data on brown meagre growth under culture conditions and contributes some basic information on its dietary protein requirement by feeding isoenergetic diets with different protein-lipid ratios by means of self-feeders. Fish with 78.8 ± 15.8 g (mean ± standard deviation) body weight were divided into four groups and subjected to four different feeding regimes with three replications for 77 days. In the first group, fish were supplied with a high protein and low fat diet (HPLF, 52: 8 w/w fat-protein ratio), the second one a medium protein and medium fat diet (MPMF, 42: 14), the third with a low protein and high fat diet (LPHF, 31: 23). In the fourth group, fish had access to both HPLF and LPHF diets. Fish fed on HPLF and MPMF diets showed significantly better growth and feed conversion ratios than fish fed on the LPHF diet. Fish with access to HPLF and LPHF diets exhibited comparable growth to the HPLF group but their feed conversion ratio was significantly higher. The condition factor and hepatosomatic index revealed no significant differences between dietary regimes. The liver of brown meagre may serve as a depository organ for energy, judging from its relatively high lipid content (39-43%). |
Chatzifotis, S; Esteban, A G; Divanach, P Fishmeal replacement by alfalfa protein concentrate in sharp snout sea bream Diplodus puntazzo Journal Article Fisheries Science, 72 (6), pp. 1313–1315, 2006, ISSN: 09199268. @article{chatzifotis_fishmeal_2006, title = {Fishmeal replacement by alfalfa protein concentrate in sharp snout sea bream Diplodus puntazzo}, author = {S Chatzifotis and A G Esteban and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33845565120&doi=10.1111%2fj.1444-2906.2006.01290.x&partnerID=40&md5=25798d024283606bd371fa82238e625c}, doi = {10.1111/j.1444-2906.2006.01290.x}, issn = {09199268}, year = {2006}, date = {2006-01-01}, journal = {Fisheries Science}, volume = {72}, number = {6}, pages = {1313--1315}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Katharios, P; Papandroulakis, N; Divanach, P Treatment of Microcotyle sp. (Monogenea) on the gills of cage-cultured red porgy, Pagrus pagrus following baths with formalin and mebendazole Journal Article Aquaculture, 251 (2-4), pp. 167–171, 2006, ISSN: 00448486. @article{katharios_treatment_2006, title = {Treatment of Microcotyle sp. (Monogenea) on the gills of cage-cultured red porgy, Pagrus pagrus following baths with formalin and mebendazole}, author = {P Katharios and N Papandroulakis and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-31344445980&doi=10.1016%2fj.aquaculture.2005.05.046&partnerID=40&md5=91ab895aa4134d0252eb1817aaaa56a2}, doi = {10.1016/j.aquaculture.2005.05.046}, issn = {00448486}, year = {2006}, date = {2006-01-01}, journal = {Aquaculture}, volume = {251}, number = {2-4}, pages = {167--171}, abstract = {Two bath treatment trials for the control of the monogenean Microcotyle sp. in the gills of cultured red porgy, Pagrus pagrus using formalin (200 ppm for 1 h) and mebendazole (400 ppm for 1 h) were conducted. Formalin was very effective removing all the parasites from the gills of the infected fish while mebendazole produced no significant result. Based on the results of the trial, formalin was selected for the treatment of a large population of heavily infected red porgies held in sea cages. The results of the treatment are discussed. © 2005 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Two bath treatment trials for the control of the monogenean Microcotyle sp. in the gills of cultured red porgy, Pagrus pagrus using formalin (200 ppm for 1 h) and mebendazole (400 ppm for 1 h) were conducted. Formalin was very effective removing all the parasites from the gills of the infected fish while mebendazole produced no significant result. Based on the results of the trial, formalin was selected for the treatment of a large population of heavily infected red porgies held in sea cages. The results of the treatment are discussed. © 2005 Elsevier B.V. All rights reserved. |
Katharios, P; Hayward, C; Papandroulakis, N; Divanach, P Pathology of Lamellodiscus spp. (Monogenea) parasitizing the gills of sharpsnout seabream and preliminary results of formalin treatment Journal Article Bulletin of the European Association of Fish Pathologists, 26 (5), pp. 196–201, 2006, ISSN: 01080288. @article{katharios_pathology_2006, title = {Pathology of Lamellodiscus spp. (Monogenea) parasitizing the gills of sharpsnout seabream and preliminary results of formalin treatment}, author = {P Katharios and C Hayward and N Papandroulakis and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33845670054&partnerID=40&md5=09814c007ac1f9256a311f1d53bc1f34}, issn = {01080288}, year = {2006}, date = {2006-01-01}, journal = {Bulletin of the European Association of Fish Pathologists}, volume = {26}, number = {5}, pages = {196--201}, abstract = {This report describes the pathological effects Lamellodiscus spp. on the gills of the sharpsnout seabream, Diplodus puntazzo and the efficacy of formalin as a treatment. Two species of Lamellodiscus were identified: Lamellodiscus ergensi and L. bidens. The attachment of parasites caused destruction, fusion and hyperplasia of the gill filaments. Formalin was effective in removing the worms when applied as a bath treatment at a concentration of 200mg/ml for one hour.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This report describes the pathological effects Lamellodiscus spp. on the gills of the sharpsnout seabream, Diplodus puntazzo and the efficacy of formalin as a treatment. Two species of Lamellodiscus were identified: Lamellodiscus ergensi and L. bidens. The attachment of parasites caused destruction, fusion and hyperplasia of the gill filaments. Formalin was effective in removing the worms when applied as a bath treatment at a concentration of 200mg/ml for one hour. |
Zaiss, M M; Papadakis, I E; Maingot, E; Divanach, P; Mylonas, C C Ontogeny of the digestive tract in shi drum (Umbrina cirrosa L.) reared using the mesocosm larval rearing system Journal Article Aquaculture, 260 (1-4), pp. 357–368, 2006, ISSN: 00448486. @article{zaiss_ontogeny_2006, title = {Ontogeny of the digestive tract in shi drum (Umbrina cirrosa L.) reared using the mesocosm larval rearing system}, author = {M M Zaiss and I E Papadakis and E Maingot and P Divanach and C C Mylonas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33747800319&doi=10.1016%2fj.aquaculture.2006.06.001&partnerID=40&md5=4f7c6eb4f3932426b7e5fb62cafa6f95}, doi = {10.1016/j.aquaculture.2006.06.001}, issn = {00448486}, year = {2006}, date = {2006-01-01}, journal = {Aquaculture}, volume = {260}, number = {1-4}, pages = {357--368}, abstract = {Histological changes of the digestive tract were studied in shi drum (Umbrina cirrosa) from hatching until 41 days post hatching (dph), when the fry had a mean (±S.D.) total length (TL) of 32 ± 2 mm and wet weight (WW) of 0.42 ± 0.07 g. Larvae were reared using the mesocosm technique, the most natural among commercially employed rearing methods for marine larvae. Shi drum opened their mouth at 2 dph (2.78 ± 0.09 mm TL), at which time 90% of the larvae already had an inflated swim bladder. The differentiation of the digestive tract into buccopharynx, esophagus, and anterior and posterior intestine was completed by 3 dph (2.82 ± 0.07 mm TL), 1 day after the onset of exogenous feeding. The alimentary canal started coiling and formed its first loop at 2 dph, while the pancreas and liver were differentiated at 3 dph. Yolk sac reserves lasted until 7 dph (4.3 ± 0.1 mm TL), suggesting a brief period of endogenous and exogenous feeding. The first esophageal goblet cells appeared at 7 dph containing acid mucins and at 8 dph taste buds appeared on the buccopharyngeal epithelium. The stomach was morphologically differentiated at 9 dph (5.5 ± 0.1 mm TL) when gastric glands became abundant in the cardiac region, and the first pyloric caeca appeared at 14 dph (10.1 ± 0.9 mm TL). Supranuclear eosinophilic vacuoles were observed in the posterior intestine between 3 and 11 dph (6.3 ± 0.9 mm TL). Their number decreased as the stomach differentiated, suggesting a change in the protein digestion mechanism. The results of the study suggest a rapid development of shi drum and its digestive system and underline the possibility of weaning larvae to artificial feed even earlier than the 12 dph employed in the present study. © 2006 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Histological changes of the digestive tract were studied in shi drum (Umbrina cirrosa) from hatching until 41 days post hatching (dph), when the fry had a mean (±S.D.) total length (TL) of 32 ± 2 mm and wet weight (WW) of 0.42 ± 0.07 g. Larvae were reared using the mesocosm technique, the most natural among commercially employed rearing methods for marine larvae. Shi drum opened their mouth at 2 dph (2.78 ± 0.09 mm TL), at which time 90% of the larvae already had an inflated swim bladder. The differentiation of the digestive tract into buccopharynx, esophagus, and anterior and posterior intestine was completed by 3 dph (2.82 ± 0.07 mm TL), 1 day after the onset of exogenous feeding. The alimentary canal started coiling and formed its first loop at 2 dph, while the pancreas and liver were differentiated at 3 dph. Yolk sac reserves lasted until 7 dph (4.3 ± 0.1 mm TL), suggesting a brief period of endogenous and exogenous feeding. The first esophageal goblet cells appeared at 7 dph containing acid mucins and at 8 dph taste buds appeared on the buccopharyngeal epithelium. The stomach was morphologically differentiated at 9 dph (5.5 ± 0.1 mm TL) when gastric glands became abundant in the cardiac region, and the first pyloric caeca appeared at 14 dph (10.1 ± 0.9 mm TL). Supranuclear eosinophilic vacuoles were observed in the posterior intestine between 3 and 11 dph (6.3 ± 0.9 mm TL). Their number decreased as the stomach differentiated, suggesting a change in the protein digestion mechanism. The results of the study suggest a rapid development of shi drum and its digestive system and underline the possibility of weaning larvae to artificial feed even earlier than the 12 dph employed in the present study. © 2006 Elsevier B.V. All rights reserved. |
Katharios, P; Garaffo, M; Sarter, K; Poulou, Athanasso F; Mylonas, C C; Divanach, P Mortality of steroid-treated sharpsnout sea bream Diplodus puntazzo caused by Ceratomyxa diplodae (Protozoa: Myxosporea). Inproceedings Fifth International Symposium on Aquatic Animal Health, pp. 148, San Francisco, U.S., 2006. @inproceedings{katharios_mortality_2006, title = {Mortality of steroid-treated sharpsnout sea bream Diplodus puntazzo caused by Ceratomyxa diplodae (Protozoa: Myxosporea).}, author = {P Katharios and M Garaffo and K Sarter and F Athanasso Poulou and C C Mylonas and P Divanach}, year = {2006}, date = {2006-01-01}, booktitle = {Fifth International Symposium on Aquatic Animal Health}, pages = {148}, address = {San Francisco, U.S.}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Pavlidis, M; Papandroulakis, N; Divanach, P A method for the comparison of chromaticity parameters in fish skin: Preliminary results for coloration pattern of red skin Sparidae Journal Article Aquaculture, 258 (1-4), pp. 211 – 219, 2006. @article{pavlidis_method_2006, title = {A method for the comparison of chromaticity parameters in fish skin: Preliminary results for coloration pattern of red skin Sparidae}, author = {M Pavlidis and N Papandroulakis and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33746956857&partnerID=40&md5=00f1e5f14d6fc540ca0f76a0817c61dd}, doi = {https://doi.org/10.1016/j.aquaculture.2006.05.028}, year = {2006}, date = {2006-01-01}, journal = {Aquaculture}, volume = {258}, number = {1-4}, pages = {211 -- 219}, abstract = {Skin coloration patterns in fish are of great physiological, behavioural and ecological importance, can be considered as an index of animal welfare in aquaculture and as an important quality factor for the retail value, especially of red skinned fish. In the present study we developed an appropriate method for comparisons of the skin chromaticity parameters in wild (Pagrus pagrus, Pagrus caeruleostictus, and Dentex gibbosus) and farmed red skin Sparidae and investigated the effect of storage time on skin colour of farmed Pagrus pagrus. The three-dimensional characteristics of colour appearance (lightness - L*, hue - H°ab and chroma -Cab *) were calculated. A new index, named Entire Colour Index (ECI), was developed to express hue and chroma which as combined variables, cannot be considered separately. In all species there was a remarkable dorsoventral gradient in mean L* and H°ab, with the ventral area being statistically significant brighter than the dorsal one. ECI value was species specific but did not show any statistically significant dorsoventral gradient, with the exception of P. pagrus. Skin melanin and carotenoids content presented differences between the Pagrus and the Dentex species that may explain the differences in the observed chromaticity pattern. Storage time affected lightness and hue only in the dorsal skin area. However, the effect of storage on ice was better reflected in mean ECI value which showed a marked decrease from Day 3 to Day 7 in both the dorsal (Day 0: 8.96 ± 0.40; Day 7: 2.86 ± 0.29) and ventral (Day 0: 8.97 ± 0.18; Day 7: 4.10 ± 0.29) skin area. Our results provide data for a non-subjective determination of skin colour pattern and show that ECI offers a good index of the actual colour in a meaningful and objective way. © 2006 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Skin coloration patterns in fish are of great physiological, behavioural and ecological importance, can be considered as an index of animal welfare in aquaculture and as an important quality factor for the retail value, especially of red skinned fish. In the present study we developed an appropriate method for comparisons of the skin chromaticity parameters in wild (Pagrus pagrus, Pagrus caeruleostictus, and Dentex gibbosus) and farmed red skin Sparidae and investigated the effect of storage time on skin colour of farmed Pagrus pagrus. The three-dimensional characteristics of colour appearance (lightness - L*, hue - H°ab and chroma -Cab *) were calculated. A new index, named Entire Colour Index (ECI), was developed to express hue and chroma which as combined variables, cannot be considered separately. In all species there was a remarkable dorsoventral gradient in mean L* and H°ab, with the ventral area being statistically significant brighter than the dorsal one. ECI value was species specific but did not show any statistically significant dorsoventral gradient, with the exception of P. pagrus. Skin melanin and carotenoids content presented differences between the Pagrus and the Dentex species that may explain the differences in the observed chromaticity pattern. Storage time affected lightness and hue only in the dorsal skin area. However, the effect of storage on ice was better reflected in mean ECI value which showed a marked decrease from Day 3 to Day 7 in both the dorsal (Day 0: 8.96 ± 0.40; Day 7: 2.86 ± 0.29) and ventral (Day 0: 8.97 ± 0.18; Day 7: 4.10 ± 0.29) skin area. Our results provide data for a non-subjective determination of skin colour pattern and show that ECI offers a good index of the actual colour in a meaningful and objective way. © 2006 Elsevier B.V. All rights reserved. |
2005 |
Sfakianakis, D G; Doxa, C K; Kouttouki, S; Koumoundouros, G; Maingot, E; Divanach, P; Kentouri, M Osteological development of the vertebral column and of the fins in Diplodus puntazzo (Cetti, 1777) Journal Article Aquaculture, 250 (1-2), pp. 36–46, 2005, ISSN: 00448486. @article{sfakianakis_osteological_2005, title = {Osteological development of the vertebral column and of the fins in Diplodus puntazzo (Cetti, 1777)}, author = {D G Sfakianakis and C K Doxa and S Kouttouki and G Koumoundouros and E Maingot and P Divanach and M Kentouri}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848605002498}, doi = {10.1016/j.aquaculture.2005.03.042}, issn = {00448486}, year = {2005}, date = {2005-11-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {250}, number = {1-2}, pages = {36--46}, abstract = {The normal development of the vertebral column and of the fins in Diplodus puntazzo (Cetti, 1777) was studied. The ontogenetic pattern of the skeleton follows that described in all other Sparidae studied so far: the onset of the ontogeny of the vertebral column is at 4.9 mm total length (TL) with the formation of the first neural processes, while the vertebral centra begin forming at 5.8 mm TL and are completed by 8.1 mm TL. Pectoral supports (cleithrum, coracoid-scapula cartilage, fin plate) are the first fin elements to be formed at 2.9 mm TL and are followed by those of the caudal (4.5 mm TL), dorsal, anal (6.4 mm TL) and finally by those of the pelvic fin (8.1 mm TL). The caudal fin is the first to develop fin rays and attain the full count of lepidotrichia (5.8-7.1 mm TL), but the last to attain the full count of all its rays (dermatotrichia, 14.1 mm TL). The next fins to complete their rays are the anal (8.5 mm TL), the dorsal (10.1 mm TL) and finally the pectoral and the pelvic (10.6 mm TL). Fin meristic characters were compared between the reared and 27 wild D. puntazzo juveniles. The results of the skeletal ontogeny are compared with the pattern of development of the Sparidae. © 2005 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The normal development of the vertebral column and of the fins in Diplodus puntazzo (Cetti, 1777) was studied. The ontogenetic pattern of the skeleton follows that described in all other Sparidae studied so far: the onset of the ontogeny of the vertebral column is at 4.9 mm total length (TL) with the formation of the first neural processes, while the vertebral centra begin forming at 5.8 mm TL and are completed by 8.1 mm TL. Pectoral supports (cleithrum, coracoid-scapula cartilage, fin plate) are the first fin elements to be formed at 2.9 mm TL and are followed by those of the caudal (4.5 mm TL), dorsal, anal (6.4 mm TL) and finally by those of the pelvic fin (8.1 mm TL). The caudal fin is the first to develop fin rays and attain the full count of lepidotrichia (5.8-7.1 mm TL), but the last to attain the full count of all its rays (dermatotrichia, 14.1 mm TL). The next fins to complete their rays are the anal (8.5 mm TL), the dorsal (10.1 mm TL) and finally the pectoral and the pelvic (10.6 mm TL). Fin meristic characters were compared between the reared and 27 wild D. puntazzo juveniles. The results of the skeletal ontogeny are compared with the pattern of development of the Sparidae. © 2005 Elsevier B.V. All rights reserved. |
Chatzifotis, S; Papadakis, I E; Divanach, P Effect of dietary water on growth of dentex Dentex dentex Journal Article Fisheries Science, 71 (6), pp. 1243–1248, 2005, ISSN: 09199268. @article{chatzifotis_effect_2005, title = {Effect of dietary water on growth of dentex Dentex dentex}, author = {S Chatzifotis and I E Papadakis and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-31144439969&doi=10.1111%2fj.1444-2906.2005.01089.x&partnerID=40&md5=8d72e22c36d27bfa837cdc0365529fd7}, doi = {10.1111/j.1444-2906.2005.01089.x}, issn = {09199268}, year = {2005}, date = {2005-01-01}, journal = {Fisheries Science}, volume = {71}, number = {6}, pages = {1243--1248}, abstract = {This study is concerned with the effect of hyrdated diets on growth and feed performance of Dentex dentex. Three levels of dietary water were tested: 7%, 20% and 40%. For the 7% level a commercial feed was used and the 20% and 40% water levels were obtained after adding an appropriate amount of deionized water to the commercial feed and mixing the pellets well. The diets were offered to fish by self-feeders, by which fish were allowed continuous access to diets after activating the self-feeders; by belt feeders, by which the diets were fed continuously and in excess over a 12 h interval; and by hand to apparent satiation two times a day. After 151 days of feeding, the group fed on the 20%-water diet showed significantly (P textless 0.05) higher growth rate than the 7 and 40% groups, while no significant differences were observed in feed conversion rate or daily feed consumption. The means of feed delivery did not influence growth rate. Self-feeder, belt and hand feeding all gave comparable growth. Fish fed the more condensed 7%-water diet had smaller stomachs than fish fed the larger volume 40%-water diet. In the stomach, all groups of fish hydrated their digesta within 2 h after feeding to a moisture content of around 60%. Apparently no difference in digestive process was observed between groups. It was concluded that the addition of water in diet at the level of 20% has a beneficial effect on dentex growth.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study is concerned with the effect of hyrdated diets on growth and feed performance of Dentex dentex. Three levels of dietary water were tested: 7%, 20% and 40%. For the 7% level a commercial feed was used and the 20% and 40% water levels were obtained after adding an appropriate amount of deionized water to the commercial feed and mixing the pellets well. The diets were offered to fish by self-feeders, by which fish were allowed continuous access to diets after activating the self-feeders; by belt feeders, by which the diets were fed continuously and in excess over a 12 h interval; and by hand to apparent satiation two times a day. After 151 days of feeding, the group fed on the 20%-water diet showed significantly (P textless 0.05) higher growth rate than the 7 and 40% groups, while no significant differences were observed in feed conversion rate or daily feed consumption. The means of feed delivery did not influence growth rate. Self-feeder, belt and hand feeding all gave comparable growth. Fish fed the more condensed 7%-water diet had smaller stomachs than fish fed the larger volume 40%-water diet. In the stomach, all groups of fish hydrated their digesta within 2 h after feeding to a moisture content of around 60%. Apparently no difference in digestive process was observed between groups. It was concluded that the addition of water in diet at the level of 20% has a beneficial effect on dentex growth. |
Chatzifotis, S; Pavlidis, M; Jimeno, C D; Vardanis, G; Sterioti, A; Divanach, P The effect of different carotenoid sources on skin coloration of cultured red porgy (Pagrus pagrus) Journal Article Aquaculture Research, 36 (15), pp. 1517–1525, 2005, ISSN: 1355557X. @article{chatzifotis_effect_2005-1, title = {The effect of different carotenoid sources on skin coloration of cultured red porgy (Pagrus pagrus)}, author = {S Chatzifotis and M Pavlidis and C D Jimeno and G Vardanis and A Sterioti and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-28144454613&doi=10.1111%2fj.1365-2109.2005.01374.x&partnerID=40&md5=70151f9b5623aeec3809a55ae9d2a95d}, doi = {10.1111/j.1365-2109.2005.01374.x}, issn = {1355557X}, year = {2005}, date = {2005-01-01}, journal = {Aquaculture Research}, volume = {36}, number = {15}, pages = {1517--1525}, abstract = {This study presents data on the effect of carotenoid sources on skin coloration of red porgy (Pagrus pagrus). Three experiments were conducted: in the first, fish were fed an astaxanthin (Naturose®)-supplemented diet, while the second fish received diets supplemented with β-carotene (Rovimix β-caroten®) or lycopene (Lyc-O-Mato®): Carotenoids were added to the level of 100 ppm in each diet, while a non-carotenoid-supplemented diet served as a control. In the third experiment, the effect of dietary protein/carbohydrate ratio on melanin content in the skin was investigated. For this experimentation, four diets were formulated to contain 50/23, 40/32, 30/48 and 20/59 protein/carbohydrate ratio. Naturose astaxanthin increased total carotenoid content in the dorsal skin area while β-carotene and lycopene seem to have had no significant effect. Naturose was the only carotenoid source that had a significant effect on skin hue, promoting a reddish coloration to the dorsal skin area and a ventral hue similar to wild red porgy. No apparent effect of carotenoid source on skin melanin content was observed. In contrast, dietary protein/ carbohydrate ratio affected melanin content in the skin. The fish fed the 50/23 diet showed significantly higher values. Farmed red porgy had eight times higher dorsal-skin melanin content than wild ones. © 2005 Blackwell Publishing Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study presents data on the effect of carotenoid sources on skin coloration of red porgy (Pagrus pagrus). Three experiments were conducted: in the first, fish were fed an astaxanthin (Naturose®)-supplemented diet, while the second fish received diets supplemented with β-carotene (Rovimix β-caroten®) or lycopene (Lyc-O-Mato®): Carotenoids were added to the level of 100 ppm in each diet, while a non-carotenoid-supplemented diet served as a control. In the third experiment, the effect of dietary protein/carbohydrate ratio on melanin content in the skin was investigated. For this experimentation, four diets were formulated to contain 50/23, 40/32, 30/48 and 20/59 protein/carbohydrate ratio. Naturose astaxanthin increased total carotenoid content in the dorsal skin area while β-carotene and lycopene seem to have had no significant effect. Naturose was the only carotenoid source that had a significant effect on skin hue, promoting a reddish coloration to the dorsal skin area and a ventral hue similar to wild red porgy. No apparent effect of carotenoid source on skin melanin content was observed. In contrast, dietary protein/ carbohydrate ratio affected melanin content in the skin. The fish fed the 50/23 diet showed significantly higher values. Farmed red porgy had eight times higher dorsal-skin melanin content than wild ones. © 2005 Blackwell Publishing Ltd. |
Katharios, P; Papadakis, I E; Prapas, A; Dermon, C R; Ampatzis, K; Divanach, P Bulletin of the European Association of Fish Pathologists, 25 (1), pp. 28–31, 2005, ISSN: 01080288. @article{katharios_mortality_2005, title = {Mortality control of viral encephalopathy and retinopathy in 0+ grouper Epinephelus marginatus after prolonged bath in dense Chlorella minutissima culture}, author = {P Katharios and I E Papadakis and A Prapas and C R Dermon and K Ampatzis and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-15744380255&partnerID=40&md5=1882681096115ce168df99dfd0aaf0d8}, issn = {01080288}, year = {2005}, date = {2005-01-01}, journal = {Bulletin of the European Association of Fish Pathologists}, volume = {25}, number = {1}, pages = {28--31}, abstract = {0+ grouper (1±0.2g), presenting symptoms of Viral Encephalopathy and Retinopathy (confirmed by isolation of the virus in SSN-1 cell line and identification by IFAT and DNA sequencing as well as by brain histology), were submitted to seven bath treatment regimes for a 30-day period, using different concentrations of Chlorella minutissima and the antibiotic furaltadone. The cumulative mortalities were 0, 0, 5 and 32% when using 2×108, 108, 5×107 and 2×107 cells of C. minutissima ml-1 respectively, compared to 31-36 % in the control and 55-68% in the antibiotic-treated groups. The beneficial effect, the possible immuno-stimulant and antiviral properties of unicellular micro-algae such as C. minutissima, as well as the role of their concentration in the bath treatment, are discussed.}, keywords = {}, pubstate = {published}, tppubtype = {article} } 0+ grouper (1±0.2g), presenting symptoms of Viral Encephalopathy and Retinopathy (confirmed by isolation of the virus in SSN-1 cell line and identification by IFAT and DNA sequencing as well as by brain histology), were submitted to seven bath treatment regimes for a 30-day period, using different concentrations of Chlorella minutissima and the antibiotic furaltadone. The cumulative mortalities were 0, 0, 5 and 32% when using 2×108, 108, 5×107 and 2×107 cells of C. minutissima ml-1 respectively, compared to 31-36 % in the control and 55-68% in the antibiotic-treated groups. The beneficial effect, the possible immuno-stimulant and antiviral properties of unicellular micro-algae such as C. minutissima, as well as the role of their concentration in the bath treatment, are discussed. |
Mylonas, C C; Anezaki, L; Divanach, P; Zanuy, S; Piferrer, F; Ron, B; Peduel, A; Atia, Ben I; Gorshkov, S; Tandler, A Journal of Fish Biology, 67 (3), pp. 652–668, 2005, ISSN: 00221112. @article{mylonas_influence_2005, title = {Influence of rearing temperature during the larval and nursery periods on growth and sex differentiation in two Mediterranean strains of Dicentrarchus labrax}, author = {C C Mylonas and L Anezaki and P Divanach and S Zanuy and F Piferrer and B Ron and A Peduel and I Ben Atia and S Gorshkov and A Tandler}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-20244367191&doi=10.1111%2fj.0022-1112.2005.00766.x&partnerID=40&md5=4bcba3bc5b4f5b44ae84310c6b854a28}, doi = {10.1111/j.0022-1112.2005.00766.x}, issn = {00221112}, year = {2005}, date = {2005-01-01}, journal = {Journal of Fish Biology}, volume = {67}, number = {3}, pages = {652--668}, abstract = {European sea bass Dicentrarchus labrax of the north-western (NW) and south-eastern (SE) Mediterranean Sea strains were exposed to different temperatures (13, 17 or 21°C) during the larval rearing (11-51 days post hatching, dph) or nursery periods (55-95 dph), in order to examine the effects of temperature on sex differentiation and subsequent growth during the first year of life. Higher growth was observed during exposure to higher temperatures, but fish of the NW strain exposed to 13 or 17°C during larval rearing exhibited compensatory growth once exposure to the lower temperatures finished, and as a result their final size at 300 dph was similar or greater to the group exposed to 21°C. Fish exposed to 17°C during the nursery period also had similar size to fish exposed to 21°C after 300 days of rearing, but the fish exposed to 13°C remained significantly smaller (ANOVA}, keywords = {}, pubstate = {published}, tppubtype = {article} } European sea bass Dicentrarchus labrax of the north-western (NW) and south-eastern (SE) Mediterranean Sea strains were exposed to different temperatures (13, 17 or 21°C) during the larval rearing (11-51 days post hatching, dph) or nursery periods (55-95 dph), in order to examine the effects of temperature on sex differentiation and subsequent growth during the first year of life. Higher growth was observed during exposure to higher temperatures, but fish of the NW strain exposed to 13 or 17°C during larval rearing exhibited compensatory growth once exposure to the lower temperatures finished, and as a result their final size at 300 dph was similar or greater to the group exposed to 21°C. Fish exposed to 17°C during the nursery period also had similar size to fish exposed to 21°C after 300 days of rearing, but the fish exposed to 13°C remained significantly smaller (ANOVA |
Koumoundouros, G; Kouttouki, S; Georgakopoulou, E; Papadakis, I; Maingot, E; Kaspiris, P; Kiriakou, Y; Georgiou, G; Divanach, P; Kentouri, M; Mylonas, C C Ontogeny of the shi drum Umbrina cirrosa (Linnaeus 1758), a candidate new species for aquaculture Journal Article Aquaculture Research, 36 (13), pp. 1265–1272, 2005, ISSN: 1355557X. @article{koumoundouros_ontogeny_2005, title = {Ontogeny of the shi drum Umbrina cirrosa (Linnaeus 1758), a candidate new species for aquaculture}, author = {G Koumoundouros and S Kouttouki and E Georgakopoulou and I Papadakis and E Maingot and P Kaspiris and Y Kiriakou and G Georgiou and P Divanach and M Kentouri and C C Mylonas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-25844487755&doi=10.1111%2fj.1365-2109.2005.01314.x&partnerID=40&md5=e26503abd5c1e3f3b24d5805f8231280}, doi = {10.1111/j.1365-2109.2005.01314.x}, issn = {1355557X}, year = {2005}, date = {2005-01-01}, journal = {Aquaculture Research}, volume = {36}, number = {13}, pages = {1265--1272}, abstract = {The ontogeny of shi drum Umbrina cirrosa (Linnaeus 1758), a candidate new species for aquaculture, was studied throughout the entire larval phase. Geometric morphometric analysis revealed two clear inflection points (7.0 and 12.7 mm total length, TL) in the shape ontogeny of this species, separating the studied period into three phases of different allometric priorities. Spline graphs demonstrated that the major non-uniform shape ontogeny correlated with the development of the fins, the anterior dorsal area of the body, the caudal peduncle, the eye and the mouth. Concerning the morphological features, shi drum larvae are characterized by an upward anterior bending of the notochord. The ontogeny of the fins began with the formation of the pectoral buds (2.9 mm TL), continued with the notochord flexion (4.3 mm TL, associated with the caudal fin development), the appearance of the pelvic buds, the first anal rays (4.5 mm TL) and the first dorsal rays (4.8 mm TL). Shi drum juveniles presented 25 vertebrae and the following dominant fin types: D XI,23, AII,6,VI,5, P17 and C17. © 2005 Blackwell Publishing Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The ontogeny of shi drum Umbrina cirrosa (Linnaeus 1758), a candidate new species for aquaculture, was studied throughout the entire larval phase. Geometric morphometric analysis revealed two clear inflection points (7.0 and 12.7 mm total length, TL) in the shape ontogeny of this species, separating the studied period into three phases of different allometric priorities. Spline graphs demonstrated that the major non-uniform shape ontogeny correlated with the development of the fins, the anterior dorsal area of the body, the caudal peduncle, the eye and the mouth. Concerning the morphological features, shi drum larvae are characterized by an upward anterior bending of the notochord. The ontogeny of the fins began with the formation of the pectoral buds (2.9 mm TL), continued with the notochord flexion (4.3 mm TL, associated with the caudal fin development), the appearance of the pelvic buds, the first anal rays (4.5 mm TL) and the first dorsal rays (4.8 mm TL). Shi drum juveniles presented 25 vertebrae and the following dominant fin types: D XI,23, AII,6,VI,5, P17 and C17. © 2005 Blackwell Publishing Ltd. |
Papadaki, M; Piferrer, F; Zanuy, S; Maingot, E; Divanach, P; Mylonas, C C Journal of Fish Biology, 66 (4), pp. 938–956, 2005, ISSN: 00221112, (Publisher: Blackwell Publishing Ltd). @article{papadaki_growth_2005, title = {Growth, sex differentiation and gonad and plasma levels of sex steroids in male- and female-dominant populations of Dicentrarchus labrax obtained through repeated size grading}, author = {M Papadaki and F Piferrer and S Zanuy and E Maingot and P Divanach and C C Mylonas}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-17244363508&doi=10.1111%2fj.0022-1112.2005.00639.x&partnerID=40&md5=818526ad19bf4809e7b643ad51c39425}, doi = {10.1111/j.0022-1112.2005.00639.x}, issn = {00221112}, year = {2005}, date = {2005-01-01}, journal = {Journal of Fish Biology}, volume = {66}, number = {4}, pages = {938--956}, abstract = {Starting from 66 days post hatching (dph), European sea bass Dicentrarchus labrax were graded successively to create a fast growing (L-extreme) and a slow growing (S-extreme) population. The L-extreme population grew significantly larger (ANOVA}, note = {Publisher: Blackwell Publishing Ltd}, keywords = {}, pubstate = {published}, tppubtype = {article} } Starting from 66 days post hatching (dph), European sea bass Dicentrarchus labrax were graded successively to create a fast growing (L-extreme) and a slow growing (S-extreme) population. The L-extreme population grew significantly larger (ANOVA |
Kokokiris, L; Canario, A; Mylonas, C; Pavlidis, M; Kentouri, M; Divanach, P Induction of ovulation and spawning in the Mediterranean Red Porgy, Pagrus Pagrus, by controlled delivery and acute injection of GnRHa Journal Article Israeli Journal of Aquaculture - Bamidgeh, 57 (4), pp. 223–230, 2005, ISSN: 0792156X. @article{kokokiris_induction_2005, title = {Induction of ovulation and spawning in the Mediterranean Red Porgy, Pagrus Pagrus, by controlled delivery and acute injection of GnRHa}, author = {L Kokokiris and A Canario and C Mylonas and M Pavlidis and M Kentouri and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-29744437550&partnerID=40&md5=fe674a5bea66c6aa820eb3a5b7d62661}, issn = {0792156X}, year = {2005}, date = {2005-01-01}, journal = {Israeli Journal of Aquaculture - Bamidgeh}, volume = {57}, number = {4}, pages = {223--230}, abstract = {Gonadotropin-releasing hormone analogue (GnRHa) in the form of saline injections or sustained-release microspheres was used to induce oocyte maturation, ovulation, and spawning in captive red porgy (Pagrus pagrus). Individually tagged vitellogenic females (n = 9 or 10) were treated at the beginning of the spawning season (March) with 20 μg/kg body weight (bw) GnRHa-loaded microspheres, a single injection of 20 μg/ kg bw dissolved in saline, or physiological saline (control). Females were placed in tanks (one tank per treatment) connected to overflow egg collectors and monitored for 11 days. In addition to the eggs collected from the tank overflow, eggs were stripped from the fish on a daily basis. Only one spawn was obtained from the control fish, probably from a single female, given the small relative fecundity (700 eggs/kg bw). On the contrary, treatment with a GnRHa injection produced two spawns (9 and 11 days after treatment) and 50% of the fish ovulated. Treatment with GnRHa microspheres induced seven spawns (3 and 6-11 days after treatment) and 100% of the females ovulated. Females did not spawn all the eggs ovulated on a particular day, evident from the significant number of eggs obtained by manual stripping. Egg quality did not significantly differ among treatments, whereas number of spawned eggs and total relative fecundity were significantly higher in fish treated with GnRHa microspheres (ANOVA, ptextless0.05). The results demonstrate the potential of GnRHa-loaded microspheres to induce spawning in red porgy, as a method of overcoming spawning failures in commercial hatcheries.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Gonadotropin-releasing hormone analogue (GnRHa) in the form of saline injections or sustained-release microspheres was used to induce oocyte maturation, ovulation, and spawning in captive red porgy (Pagrus pagrus). Individually tagged vitellogenic females (n = 9 or 10) were treated at the beginning of the spawning season (March) with 20 μg/kg body weight (bw) GnRHa-loaded microspheres, a single injection of 20 μg/ kg bw dissolved in saline, or physiological saline (control). Females were placed in tanks (one tank per treatment) connected to overflow egg collectors and monitored for 11 days. In addition to the eggs collected from the tank overflow, eggs were stripped from the fish on a daily basis. Only one spawn was obtained from the control fish, probably from a single female, given the small relative fecundity (700 eggs/kg bw). On the contrary, treatment with a GnRHa injection produced two spawns (9 and 11 days after treatment) and 50% of the fish ovulated. Treatment with GnRHa microspheres induced seven spawns (3 and 6-11 days after treatment) and 100% of the females ovulated. Females did not spawn all the eggs ovulated on a particular day, evident from the significant number of eggs obtained by manual stripping. Egg quality did not significantly differ among treatments, whereas number of spawned eggs and total relative fecundity were significantly higher in fish treated with GnRHa microspheres (ANOVA, ptextless0.05). The results demonstrate the potential of GnRHa-loaded microspheres to induce spawning in red porgy, as a method of overcoming spawning failures in commercial hatcheries. |
Papandroulakis, N; Mylonas, C C; Maingot, E; Divanach, P First results of greater amberjack (Seriola dumerili) larval rearing in mesocosm Journal Article Aquaculture, 250 (1-2), pp. 155–161, 2005, ISSN: 00448486. @article{papandroulakis_first_2005, title = {First results of greater amberjack (Seriola dumerili) larval rearing in mesocosm}, author = {N Papandroulakis and C C Mylonas and E Maingot and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-27344450721&doi=10.1016%2fj.aquaculture.2005.02.036&partnerID=40&md5=85aba7698333eb1cdf77b60229a40d1b}, doi = {10.1016/j.aquaculture.2005.02.036}, issn = {00448486}, year = {2005}, date = {2005-01-01}, journal = {Aquaculture}, volume = {250}, number = {1-2}, pages = {155--161}, abstract = {Species diversification is considered a major approach for the sustainable development of aquaculture. The greater amberjack (Seriola dumerili) has particular characteristics-advantages making it an appropriate candidate: high growth rate, large size, and established worldwide market. In the present study, the mesocosm method for larval rearing was applied, since it has been shown to be effective in the larval rearing of several species so far. This method is a semi-intensive technology, based on daily exogenous food addition, but also having the capacity of some endogenous productivity. Greater amberjack eggs were obtained from wild-caught fish matured in captivity in 6 years, after induced spawning with implants containing gonadotropin-releasing hormone agonist (GnRHa). A total of 9800 eggs survived after embryo appearance and were incubated in a 40-m3 tank with natural seawater of 40 psu. Rearing lasted 40 days. After mouth opening on day 2 post hatching, exogenous feeding with rotifers, Artemia nauplii and inert feed was initiated, while endogenous produced copepods contributed as food for the larvae from day 7 post hatching onwards. During rearing, larvae grew with an exponential rate of 0.073 day -1 in terms of total length (TL), and reached 39.9 ± 5.4 mm and 0.5 ± 0.1 g body weight at the end of the trial. All larvae inflated their swim bladder and completed metamorphosis at about 5 mm and 8 mm TL, respectively. Schooling behavior was first observed when larvae reached 9-10 mm TL, while aggression against the smallest individuals was first noticed the same period. The final population of about 350 individuals (3.5% survival) was transferred at the end of the trial for subsequent on-growing. The results obtained indicate the reliability of the technology for the larval rearing of the greater amberjack, and also its appropriateness for diversification with difficult marine species. © 2005 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Species diversification is considered a major approach for the sustainable development of aquaculture. The greater amberjack (Seriola dumerili) has particular characteristics-advantages making it an appropriate candidate: high growth rate, large size, and established worldwide market. In the present study, the mesocosm method for larval rearing was applied, since it has been shown to be effective in the larval rearing of several species so far. This method is a semi-intensive technology, based on daily exogenous food addition, but also having the capacity of some endogenous productivity. Greater amberjack eggs were obtained from wild-caught fish matured in captivity in 6 years, after induced spawning with implants containing gonadotropin-releasing hormone agonist (GnRHa). A total of 9800 eggs survived after embryo appearance and were incubated in a 40-m3 tank with natural seawater of 40 psu. Rearing lasted 40 days. After mouth opening on day 2 post hatching, exogenous feeding with rotifers, Artemia nauplii and inert feed was initiated, while endogenous produced copepods contributed as food for the larvae from day 7 post hatching onwards. During rearing, larvae grew with an exponential rate of 0.073 day -1 in terms of total length (TL), and reached 39.9 ± 5.4 mm and 0.5 ± 0.1 g body weight at the end of the trial. All larvae inflated their swim bladder and completed metamorphosis at about 5 mm and 8 mm TL, respectively. Schooling behavior was first observed when larvae reached 9-10 mm TL, while aggression against the smallest individuals was first noticed the same period. The final population of about 350 individuals (3.5% survival) was transferred at the end of the trial for subsequent on-growing. The results obtained indicate the reliability of the technology for the larval rearing of the greater amberjack, and also its appropriateness for diversification with difficult marine species. © 2005 Elsevier B.V. All rights reserved. |
Bahri-Sfar, Lilia; Lemaire, Christophe; Chatain, Béatrice; Divanach, Pascal; Hassine, Oum Kalthoum Ben; Bonhomme, François Impact of aquaculture on the genetic structure of mediterranean populations of Dicentrarchus labrax Journal Article Aquatic Living Resources, 18 (1), pp. 71–76, 2005, ISSN: 0990-7440, 1765-2952, (Original title (in French): Impact de l'élevage sur la structure génétique des populations méditerranéennes de Dicentrarchus labrax). @article{bahri-sfar_impact_2005, title = {Impact of aquaculture on the genetic structure of mediterranean populations of Dicentrarchus labrax}, author = {Lilia Bahri-Sfar and Christophe Lemaire and Béatrice Chatain and Pascal Divanach and Oum Kalthoum Ben Hassine and François Bonhomme}, url = {http://www.alr-journal.org/10.1051/alr:2005007}, doi = {10.1051/alr:2005007}, issn = {0990-7440, 1765-2952}, year = {2005}, date = {2005-01-01}, urldate = {2020-08-18}, journal = {Aquatic Living Resources}, volume = {18}, number = {1}, pages = {71--76}, note = {Original title (in French): Impact de l'élevage sur la structure génétique des populations méditerranéennes de Dicentrarchus labrax}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2004 |
P., Katharios; I.E., Papadakis; A., Prapas; C.R., Dermon; K., Ampatzis; Divanach, P Mortality control of VNN disease in 0+ grouper, Epinephelus marginatus after prolonged bath in dense Chlorella minutissima culture. Inproceedings EAS, (Ed.): European Aquaculture Society, Biotechnologies for Quality, Barcelona, Spain, 2004. @inproceedings{katharios_p_mortality_2004, title = {Mortality control of VNN disease in 0+ grouper, Epinephelus marginatus after prolonged bath in dense Chlorella minutissima culture.}, author = {Katharios P. and Papadakis I.E. and Prapas A. and Dermon C.R. and Ampatzis K. and P Divanach}, editor = {EAS}, year = {2004}, date = {2004-10-01}, booktitle = {European Aquaculture Society, Biotechnologies for Quality}, address = {Barcelona, Spain}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Koumoundouros, G; Carrillo, J; Divanach, P; Kentouri, M The rearing of common dentex Dentex dentex (L.) During the hatchery and on-growing phases Journal Article Aquaculture, 240 (1-4), pp. 165–173, 2004, ISSN: 00448486. @article{koumoundouros_rearing_2004, title = {The rearing of common dentex Dentex dentex (L.) During the hatchery and on-growing phases}, author = {G Koumoundouros and J Carrillo and P Divanach and M Kentouri}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848604000869}, doi = {10.1016/j.aquaculture.2004.01.038}, issn = {00448486}, year = {2004}, date = {2004-10-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {240}, number = {1-4}, pages = {165--173}, abstract = {In the current paper we present the rearing methodology of common dentex (Dentex dentex), a promising candidate for Mediterranean marine aquaculture, from the larval to the on-growing phase. The results are discussed in terms of the efficiency of rearing methodology followed, for the mass production of the species. © 2004 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In the current paper we present the rearing methodology of common dentex (Dentex dentex), a promising candidate for Mediterranean marine aquaculture, from the larval to the on-growing phase. The results are discussed in terms of the efficiency of rearing methodology followed, for the mass production of the species. © 2004 Elsevier B.V. All rights reserved. |
Sfakianakis, D G; Koumoundouros, G; Divanach, P; Kentouri, M Aquaculture, 232 (1-4), pp. 407–424, 2004, ISSN: 00448486. @article{sfakianakis_osteological_2004, title = {Osteological development of the vertebral column and of the fins in Pagellus erythrinus (L. 1758). Temperature effect on the developmental plasticity and morpho-anatomical abnormalities}, author = {D G Sfakianakis and G Koumoundouros and P Divanach and M Kentouri}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848603005623}, doi = {10.1016/j.aquaculture.2003.08.014}, issn = {00448486}, year = {2004}, date = {2004-04-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {232}, number = {1-4}, pages = {407--424}, abstract = {The osteological ontogeny of Pagellus erythrinus (Linnaeus, 1758) and its temperature-induced plasticity is studied. The ontogeny of the skeleton follows the pattern described in all other Sparidae studied so far: the onset of the ontogeny of the vertebral column and the caudal fin is at 4.3 mm total length (TL) and the completion at 17.6 and 15.4 mm TL, respectively. Dorsal and anal fin development appears at about 5.2 mm TL and completes at 7.5 mm TL. The pelvic are the last fins to develop (7.5-11.5 mm TL). Although the pectorals are the only fins present before first feeding, their ontogeny is completed only after 12.0 mm TL. Temperature affects the developmental rate of the skeleton giving as much as 10.9% of differentiation in total length between 16 and 21°C (caudal lepidotrichia), 9.2% between 16 and 18°C (caudal lepidotrichia) and 6.7% between 18 and 21°C (pelvic lepidotrichia). The role of temperature in developmental plasticity is enhanced by the induction of abnormalities mainly in the area of the caudal fin, which reached (in total) 75% occurrence in the higher temperature regime (23°C) tested. The results of the skeletal ontogeny are compared with the pattern of development of the Sparidae, and are discussed in respect to the role of temperature in the developmental plasticity of fish. © 2004 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The osteological ontogeny of Pagellus erythrinus (Linnaeus, 1758) and its temperature-induced plasticity is studied. The ontogeny of the skeleton follows the pattern described in all other Sparidae studied so far: the onset of the ontogeny of the vertebral column and the caudal fin is at 4.3 mm total length (TL) and the completion at 17.6 and 15.4 mm TL, respectively. Dorsal and anal fin development appears at about 5.2 mm TL and completes at 7.5 mm TL. The pelvic are the last fins to develop (7.5-11.5 mm TL). Although the pectorals are the only fins present before first feeding, their ontogeny is completed only after 12.0 mm TL. Temperature affects the developmental rate of the skeleton giving as much as 10.9% of differentiation in total length between 16 and 21°C (caudal lepidotrichia), 9.2% between 16 and 18°C (caudal lepidotrichia) and 6.7% between 18 and 21°C (pelvic lepidotrichia). The role of temperature in developmental plasticity is enhanced by the induction of abnormalities mainly in the area of the caudal fin, which reached (in total) 75% occurrence in the higher temperature regime (23°C) tested. The results of the skeletal ontogeny are compared with the pattern of development of the Sparidae, and are discussed in respect to the role of temperature in the developmental plasticity of fish. © 2004 Elsevier B.V. All rights reserved. |
Martinez, Brotons J; Chatzifotis, S; Divanach, P; Takeuchi, T Effect of dietary taurine supplementation on growth performance and feed selection of sea bass Dicentrarchus labrax fry fed with demand-feeders Journal Article Fisheries Science, 70 (1), pp. 74–79, 2004, ISSN: 09199268. @article{brotons_martinez_effect_2004, title = {Effect of dietary taurine supplementation on growth performance and feed selection of sea bass Dicentrarchus labrax fry fed with demand-feeders}, author = {J Brotons Martinez and S Chatzifotis and P Divanach and T Takeuchi}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-1542381102&doi=10.1111%2fj.1444-2906.2003.00773.x&partnerID=40&md5=3c8ecc80e8dfafd48e7224dbfaacd7ea}, doi = {10.1111/j.1444-2906.2003.00773.x}, issn = {09199268}, year = {2004}, date = {2004-01-01}, journal = {Fisheries Science}, volume = {70}, number = {1}, pages = {74--79}, abstract = {Two series of experiments were conducted for the evaluation of the effect of dietary taurine supplementation on survival, growth performance and feed preference of sea bass Dicentrarchus labrax fry when fed with demand-feeders a fish/soybean meal-based diet. In the first experiment, fish were imposed one of four diets supplemented with taurine (0, 0.1, 0.2 or 0.3% taurine on a dry weight basis), while in the second they had simultaneous free access to the four diets supplemented with taurine. When not allowed to choose, fish exhibited better specific growth rate of 3.2 and 3.2% per day with diets supplemented with 0.2 and 0.3% taurine than those of 2.3 and 2.2% per day of O and 0.1% taurine, respectively. When fish were free to access the diets, they selected more actively the 0.2 and 0.3% taurine-supplemented diets than the 0 and 0.1% taurine. As survival was not significantly different among trials, the results indicate that sea bass fry require a 0.2% taurine in the diet for better growth when fish meal and soybean meal are the primary sources of protein. The positive correlation between growth performance and nutrient preference is discussed as a method for improvement of fry food formulation.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Two series of experiments were conducted for the evaluation of the effect of dietary taurine supplementation on survival, growth performance and feed preference of sea bass Dicentrarchus labrax fry when fed with demand-feeders a fish/soybean meal-based diet. In the first experiment, fish were imposed one of four diets supplemented with taurine (0, 0.1, 0.2 or 0.3% taurine on a dry weight basis), while in the second they had simultaneous free access to the four diets supplemented with taurine. When not allowed to choose, fish exhibited better specific growth rate of 3.2 and 3.2% per day with diets supplemented with 0.2 and 0.3% taurine than those of 2.3 and 2.2% per day of O and 0.1% taurine, respectively. When fish were free to access the diets, they selected more actively the 0.2 and 0.3% taurine-supplemented diets than the 0 and 0.1% taurine. As survival was not significantly different among trials, the results indicate that sea bass fry require a 0.2% taurine in the diet for better growth when fish meal and soybean meal are the primary sources of protein. The positive correlation between growth performance and nutrient preference is discussed as a method for improvement of fry food formulation. |
Atienza, M T; Chatzifotis, S; Divanach, P Macronutrient selection by sharp snout seabream (Diplodus puntazzo) Journal Article Aquaculture, 232 (1-4), pp. 481–491, 2004, ISSN: 00448486. @article{atienza_macronutrient_2004, title = {Macronutrient selection by sharp snout seabream (Diplodus puntazzo)}, author = {M T Atienza and S Chatzifotis and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-1542321232&doi=10.1016%2fS0044-8486%2803%2900468-X&partnerID=40&md5=7f2fd0488cac53aa1a23cefd86f2ffa5}, doi = {10.1016/S0044-8486(03)00468-X}, issn = {00448486}, year = {2004}, date = {2004-01-01}, journal = {Aquaculture}, volume = {232}, number = {1-4}, pages = {481--491}, abstract = {This study is concerned with the investigation of feed preference in juvenile sharp snout seabream (Diplodus puntazzo). Three isoenergetic diets, high protein-low fat (HPLF), medium protein and fat (MPMF) and high fat-low protein (LPHF) were tested using six feeding regimes. Three groups of fish were used as controls to test the diets independently, while to the other three, a different diet combination was provided (HPLF-MPMF, MPMF-LPHF and HPLF-LPHF). Diets were provided by means of one or two self-feeders and fish had continuous access to one or two diets. Fish had an initial weight of 11.1±3.8 g (mean±S.D.) and after 45 days of feeding, no significant differences (Ptextless0.05) was observed in specific growth rate (SGR), feed conversion ratio (FCR) and protein efficiency ratio (PER) among different groups. Fish on HPLF-MPMF and HPLF-LPHF feeding regimes showed the highest growth rate of 1.96% per day and the lowest feed conversion ratio of 1.09, respectively. The result obtained showed that although there was different availability of diets among groups, macronutrient consumption appears to converge suggesting a target intake (47% protein and 10% fat content). The protein content of the diet influenced feed consumption, growth and feed conversion ratio. © 2004 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study is concerned with the investigation of feed preference in juvenile sharp snout seabream (Diplodus puntazzo). Three isoenergetic diets, high protein-low fat (HPLF), medium protein and fat (MPMF) and high fat-low protein (LPHF) were tested using six feeding regimes. Three groups of fish were used as controls to test the diets independently, while to the other three, a different diet combination was provided (HPLF-MPMF, MPMF-LPHF and HPLF-LPHF). Diets were provided by means of one or two self-feeders and fish had continuous access to one or two diets. Fish had an initial weight of 11.1±3.8 g (mean±S.D.) and after 45 days of feeding, no significant differences (Ptextless0.05) was observed in specific growth rate (SGR), feed conversion ratio (FCR) and protein efficiency ratio (PER) among different groups. Fish on HPLF-MPMF and HPLF-LPHF feeding regimes showed the highest growth rate of 1.96% per day and the lowest feed conversion ratio of 1.09, respectively. The result obtained showed that although there was different availability of diets among groups, macronutrient consumption appears to converge suggesting a target intake (47% protein and 10% fat content). The protein content of the diet influenced feed consumption, growth and feed conversion ratio. © 2004 Elsevier B.V. All rights reserved. |
Mylonas, C C; Papadaki, M; Pavlidis, M; Divanach, P Evaluation of egg production and quality in the Mediterranean red porgy (Pagrus pagrus) during two consecutive spawning seasons Journal Article Aquaculture, 232 (1-4), pp. 637–649, 2004, ISSN: 00448486. @article{mylonas_evaluation_2004, title = {Evaluation of egg production and quality in the Mediterranean red porgy (Pagrus pagrus) during two consecutive spawning seasons}, author = {C C Mylonas and M Papadaki and M Pavlidis and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-1542321216&doi=10.1016%2fS0044-8486%2803%2900534-9&partnerID=40&md5=bb798427551c7d2bdbf2d0812cf57e45}, doi = {10.1016/S0044-8486(03)00534-9}, issn = {00448486}, year = {2004}, date = {2004-01-01}, journal = {Aquaculture}, volume = {232}, number = {1-4}, pages = {637--649}, abstract = {Egg production from two broodstocks of captive-reared red porgy (Pagrus pagrus) was monitored for two consecutive seasons (2001 and 2002). Spawning lasted from January to April-May under water temperature between 15.8 and 19.2°C. Spawning was mostly daily, though over the course of the two spawning seasons, the mean interval was 1.5-1.2 days spawn-1. There was a large variation in daily fecundity without a consistent trend, except for a slight peak in the middle of the spawning season. Mean (S.E.M.) annual relative fecundity ranged between 408,300 (95,800) and 442,500 (180,800) eggs kg-1 year-1. Mean fertilization success increased significantly from 36.9 (2.0)% in 2001 to 68.9 (2.5)% in 2002, resulting in mean annual viable egg production of 174,800 (87,600) and 301,500 (99,800) eggs kg-1, respectively. There were no significant differences between the 2 years in other egg quality parameters, which ranged between 74.5 and 79. 3% 1-day embryo survival, 81.6-85.5% hatching success and 76.7-78.8% 5-day larval survival. Of the relations examined, fertilization % had a significantly positive correlation with 1-day embryo survival, which in turn was significantly correlated with hatching %. Larval survival was further correlated with hatching %. These data suggest that decisions on whether to proceed with the incubation of a batch of eggs and rearing of the larvae can be made within 1 day from egg collection, before much effort is invested by the hatchery. The present study presents further information on the spawning kinetics, and egg production and quality characteristics of the Mediterranean red porgy in culture, and demonstrates the suitability of this species for reproduction in captivity. © 2004 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Egg production from two broodstocks of captive-reared red porgy (Pagrus pagrus) was monitored for two consecutive seasons (2001 and 2002). Spawning lasted from January to April-May under water temperature between 15.8 and 19.2°C. Spawning was mostly daily, though over the course of the two spawning seasons, the mean interval was 1.5-1.2 days spawn-1. There was a large variation in daily fecundity without a consistent trend, except for a slight peak in the middle of the spawning season. Mean (S.E.M.) annual relative fecundity ranged between 408,300 (95,800) and 442,500 (180,800) eggs kg-1 year-1. Mean fertilization success increased significantly from 36.9 (2.0)% in 2001 to 68.9 (2.5)% in 2002, resulting in mean annual viable egg production of 174,800 (87,600) and 301,500 (99,800) eggs kg-1, respectively. There were no significant differences between the 2 years in other egg quality parameters, which ranged between 74.5 and 79. 3% 1-day embryo survival, 81.6-85.5% hatching success and 76.7-78.8% 5-day larval survival. Of the relations examined, fertilization % had a significantly positive correlation with 1-day embryo survival, which in turn was significantly correlated with hatching %. Larval survival was further correlated with hatching %. These data suggest that decisions on whether to proceed with the incubation of a batch of eggs and rearing of the larvae can be made within 1 day from egg collection, before much effort is invested by the hatchery. The present study presents further information on the spawning kinetics, and egg production and quality characteristics of the Mediterranean red porgy in culture, and demonstrates the suitability of this species for reproduction in captivity. © 2004 Elsevier B.V. All rights reserved. |
Mylonas, C C; Kyriakou, Y; Sigelaki, I; Georgiou, G; Stephanou, D; Divanach, P Reproductive biology of the shi drum (Umrina cirrosa) in captivity and induction of spawning using GNRHA Journal Article Israeli Journal of Aquaculture - Bamidgeh, 56 (2), pp. 77–94, 2004, ISSN: 0792156X. @article{mylonas_reproductive_2004, title = {Reproductive biology of the shi drum (Umrina cirrosa) in captivity and induction of spawning using GNRHA}, author = {C C Mylonas and Y Kyriakou and I Sigelaki and G Georgiou and D Stephanou and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-3142760090&partnerID=40&md5=9f2ed2f2e75086d9b738a1ab4dedd38d}, issn = {0792156X}, year = {2004}, date = {2004-01-01}, journal = {Israeli Journal of Aquaculture - Bamidgeh}, volume = {56}, number = {2}, pages = {77--94}, abstract = {The reproductive biology of the shi drum (Umbrina cirrosa) in culture was histologically examined and sperm quality was monitored during an entire reproductive period. Already in April, the ovary contained oocytes in all stages of maturation, from primary oocytes to full vitellogenesis, as expected from a group-synchronous multiple-batch spawning fish. Vitellogenesis of the first batch of oocytes occurred very rapidly and their mean diameter (500 μm) did not increase significantly (p>0.05) as the reproductive period proceeded. The spermiation index peaked in May-June, but fish never produced copious amounts of milt upon abdominal pressure. The spermatozoa motility percentage remained unchanged throughout the spawning season (80%) and a significant percentage (40%) maintained viability after overnight storage at 4°C. Sperm density and motility duration increased during the reproductive period and varied 13-26 × 109 spermatozoa/ml and 26-40 s, respectively. Spontaneous spawning was not observed during the two-year study. Injection of post-vitellogenic females with an agonist of gonadotropin-releasing hormone (GnRHa) was successful in inducing a single spawning after two days, with fertilization, hatching and 4-day larval survival rates of 65%, 42-76% and 46-80%, respectively. The results underline the failure of female shi drum in culture to undergo final oocyte maturation and, although GnRHa injection was effective in inducing spawning of viable eggs, multiple treatments did not induce multiple spawns, as was expected from fish with multiple-batch group-synchronous ovarian biology.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The reproductive biology of the shi drum (Umbrina cirrosa) in culture was histologically examined and sperm quality was monitored during an entire reproductive period. Already in April, the ovary contained oocytes in all stages of maturation, from primary oocytes to full vitellogenesis, as expected from a group-synchronous multiple-batch spawning fish. Vitellogenesis of the first batch of oocytes occurred very rapidly and their mean diameter (500 μm) did not increase significantly (p>0.05) as the reproductive period proceeded. The spermiation index peaked in May-June, but fish never produced copious amounts of milt upon abdominal pressure. The spermatozoa motility percentage remained unchanged throughout the spawning season (80%) and a significant percentage (40%) maintained viability after overnight storage at 4°C. Sperm density and motility duration increased during the reproductive period and varied 13-26 × 109 spermatozoa/ml and 26-40 s, respectively. Spontaneous spawning was not observed during the two-year study. Injection of post-vitellogenic females with an agonist of gonadotropin-releasing hormone (GnRHa) was successful in inducing a single spawning after two days, with fertilization, hatching and 4-day larval survival rates of 65%, 42-76% and 46-80%, respectively. The results underline the failure of female shi drum in culture to undergo final oocyte maturation and, although GnRHa injection was effective in inducing spawning of viable eggs, multiple treatments did not induce multiple spawns, as was expected from fish with multiple-batch group-synchronous ovarian biology. |
Mylonas, C C; Papandroulakis, N; Smboukis, A; Papadaki, M; Divanach, P Induction of spawning of cultured greater amberjack (Seriola dumerili) using GnRHa implants Journal Article Aquaculture, 237 (1-4), pp. 141–154, 2004, ISSN: 00448486. @article{mylonas_induction_2004, title = {Induction of spawning of cultured greater amberjack (Seriola dumerili) using GnRHa implants}, author = {C C Mylonas and N Papandroulakis and A Smboukis and M Papadaki and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-3142566876&doi=10.1016%2fj.aquaculture.2004.04.015&partnerID=40&md5=1d5f2c1607615bf23df2ad0e72aaa783}, doi = {10.1016/j.aquaculture.2004.04.015}, issn = {00448486}, year = {2004}, date = {2004-01-01}, journal = {Aquaculture}, volume = {237}, number = {1-4}, pages = {141--154}, abstract = {The objective of the current study was to examine the possibility of maturing Mediterranean greater amberjack (Seriola dumerili) in culture at two different facilities in Greece and inducing spawning using gonadotropin releasing-hormone agonist (GnRHa) implants. Males in both stocks (n=8) were in spermiating condition in mid June, though sperm motility was low ranging between 5% and 30%, while motility duration ranged between 2.1 and 2.5 min. Females of the first stock (n=4) at the same time had ovaries with either primary oocytes or early vitellogenic oocytes at advanced stages of atresia, indicating a failure in oogenesis. On the contrary, the only female of the second stock contained oocytes at all stages of oogenesis, including early final oocyte maturation (FOM; oocyte diameter 650 μm). Implantation with a GnRHa implant induced the first spawn about 36 h later. In the absence of another spawn, a gonadal biopsy was taken 15 days later, indicating the existence of more oocytes at post vitellogenesis and some with almost complete lipid droplet coalescence. A second GnRHa implantation at this time resulted in consecutive egg releases after 36 h, 4 and 5 days, with fertilized eggs only at 36 h and 5 days. The study underlines the existence of important reproductive dysfunctions of greater amberjack in captivity, and demonstrates that GnRHa implants can be used for the induction of multiple spawns of viable eggs. © 2004 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The objective of the current study was to examine the possibility of maturing Mediterranean greater amberjack (Seriola dumerili) in culture at two different facilities in Greece and inducing spawning using gonadotropin releasing-hormone agonist (GnRHa) implants. Males in both stocks (n=8) were in spermiating condition in mid June, though sperm motility was low ranging between 5% and 30%, while motility duration ranged between 2.1 and 2.5 min. Females of the first stock (n=4) at the same time had ovaries with either primary oocytes or early vitellogenic oocytes at advanced stages of atresia, indicating a failure in oogenesis. On the contrary, the only female of the second stock contained oocytes at all stages of oogenesis, including early final oocyte maturation (FOM; oocyte diameter 650 μm). Implantation with a GnRHa implant induced the first spawn about 36 h later. In the absence of another spawn, a gonadal biopsy was taken 15 days later, indicating the existence of more oocytes at post vitellogenesis and some with almost complete lipid droplet coalescence. A second GnRHa implantation at this time resulted in consecutive egg releases after 36 h, 4 and 5 days, with fertilized eggs only at 36 h and 5 days. The study underlines the existence of important reproductive dysfunctions of greater amberjack in captivity, and demonstrates that GnRHa implants can be used for the induction of multiple spawns of viable eggs. © 2004 Elsevier B.V. All rights reserved. |
Papandroulakis, N; Kentouri, M; Divanach, P Biological performance of red porgy (Pagrus pagrus) larvae under intensive rearing conditions with the use of an automated feeding system Journal Article Aquaculture International, 12 , pp. 191 – 203, 2004. @article{papandroulakis_biological_2004, title = {Biological performance of red porgy (Pagrus pagrus) larvae under intensive rearing conditions with the use of an automated feeding system}, author = {N Papandroulakis and M Kentouri and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-3042549353&partnerID=40&md5=f683700be4763c4677be7bf71340bc32}, year = {2004}, date = {2004-01-01}, journal = {Aquaculture International}, volume = {12}, pages = {191 -- 203}, abstract = {Pagrus pagrus is one of the promising species for the industry in the Mediterranean but its rearing is still far from satisfactory. In the present work, the conditions and results of larval rearing with the use of an automatic feeding system are presented. Eight populations were reared for 20 days with the ’pseudo green water’ methodology in two successive trials. Larvae were fed enriched rotifers during the entire rearing period, a mixed diet of rotifers and Artemia (Instar I when larvae reached 5 mm in length, replaced by Instar II after larvae reached 5.5 mm) and a formulated diet (after day 16 post hatching). A computerized system for feeding management was used. A total of 388,000 eggs were incubated and after 20 days, 237,973 larvae (TL = 6.96 ± 0.17 mm) were produced with a survival rate of 61.4 ± 6.3%. Individuals grew in terms of wet weight with an exponential rate of 0.167 ± 0.008 daily (R2 = 0.983) with no variations between replicates. Mean individual daily consumption at first feeding (day 4-post hatching), was 0.20 ± 0.06 mg of food (0.03 ± 0.01 dry weight), that is, 180-300 rotifers, while on day 20, consumption increased by 10 (2.081 ± 0.106 mg and 0.276 ± 0.014 in wet and dry weight, respectively). When compared with sea bream, consumption was higher by approximately 3-4-fold. The mean food conversion ratio of the experimental period was 4. In comparison with sea bream larviculture, food consumption of red porgy is higher, and a different feeding strategy is required to satisfy the requirements of the larvae. © 2004 Kluwer Academic Publishers.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Pagrus pagrus is one of the promising species for the industry in the Mediterranean but its rearing is still far from satisfactory. In the present work, the conditions and results of larval rearing with the use of an automatic feeding system are presented. Eight populations were reared for 20 days with the ’pseudo green water’ methodology in two successive trials. Larvae were fed enriched rotifers during the entire rearing period, a mixed diet of rotifers and Artemia (Instar I when larvae reached 5 mm in length, replaced by Instar II after larvae reached 5.5 mm) and a formulated diet (after day 16 post hatching). A computerized system for feeding management was used. A total of 388,000 eggs were incubated and after 20 days, 237,973 larvae (TL = 6.96 ± 0.17 mm) were produced with a survival rate of 61.4 ± 6.3%. Individuals grew in terms of wet weight with an exponential rate of 0.167 ± 0.008 daily (R2 = 0.983) with no variations between replicates. Mean individual daily consumption at first feeding (day 4-post hatching), was 0.20 ± 0.06 mg of food (0.03 ± 0.01 dry weight), that is, 180-300 rotifers, while on day 20, consumption increased by 10 (2.081 ± 0.106 mg and 0.276 ± 0.014 in wet and dry weight, respectively). When compared with sea bream, consumption was higher by approximately 3-4-fold. The mean food conversion ratio of the experimental period was 4. In comparison with sea bream larviculture, food consumption of red porgy is higher, and a different feeding strategy is required to satisfy the requirements of the larvae. © 2004 Kluwer Academic Publishers. |
Papandroulakis, N; Suquet, M; Spedicato, M T; Machias, A; Fauvel, C; Divanach, P Feeding rates, growth performance and gametogenesis of wreckfish (Polyprion americanus) kept in captivity Journal Article Aquaculture International, 12 , pp. 395 – 407, 2004. @article{papandroulakis_feeding_2004, title = {Feeding rates, growth performance and gametogenesis of wreckfish (Polyprion americanus) kept in captivity}, author = {N Papandroulakis and M Suquet and M T Spedicato and A Machias and C Fauvel and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33749494732&partnerID=40&md5=452a6a205ec500f8fdcd8736ff3a50f1}, year = {2004}, date = {2004-01-01}, journal = {Aquaculture International}, volume = {12}, pages = {395 -- 407}, abstract = {Wreckfish (Polyprion americanus) is a panoceanic, antitropical distributed species. Despite the high market price, high growth rate, good quality flesh and the potential for aquaculture, research data are scarce. In this study, the growth performance and food intake was investigated in juvenile fish, while gametogenesis was studied in adult fish. Three groups were created from 19 individuals, which were collected during neustonic sampling, using fish aggregating devices, in association with floating objects and during tuna fishing with drift nets from Greece (Crete), Italy (Ionian Sea) and the Atlantic coast of France from September 1999 to March 2001. All individuals (initial weight between 0.5 g and 4.5kg) were reared in aquaculture facilities so as to monitor growth performance and food intake. Food (de-frozen fish - bogue, mackerel and squid - or moist pellets) was delivered ad libitum. Total length and wet weight were measured regularly. Daily food intake, the food conversion index and daily growth rate were calculated. Individuals, aging 0+, grew exceptionally fast, reaching 2.0kg (total length 50cm) in 12 months and 5.0kg (total length 65cm) in 24, while at later stages, the growth rate decreased and depended on the water temperature during rearing. Food consumption varied during the rearing period according to the developmental stage and the rearing conditions. The food conversion ratio ranged between 0.9 and 2.5 on dry weight basis during the period of the study. Gametogenesis was monitored in a separate stock of 15 individuals (11.7 ± 3.7 kg) by biopsis while blood samples were taken for steroid estimation. Increased oocyte diameter was observed in females from August to January, while plasma estradiol levels also increased from October to March. However, neither egg or sperm were collected from the stock. Young wreckfish presented a high growth rate in captivity, thus supporting the feasibility of the species for rearing. Further research, however, is required for the reproduction of the species. © 2004 Kluwer Academic Publishers.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Wreckfish (Polyprion americanus) is a panoceanic, antitropical distributed species. Despite the high market price, high growth rate, good quality flesh and the potential for aquaculture, research data are scarce. In this study, the growth performance and food intake was investigated in juvenile fish, while gametogenesis was studied in adult fish. Three groups were created from 19 individuals, which were collected during neustonic sampling, using fish aggregating devices, in association with floating objects and during tuna fishing with drift nets from Greece (Crete), Italy (Ionian Sea) and the Atlantic coast of France from September 1999 to March 2001. All individuals (initial weight between 0.5 g and 4.5kg) were reared in aquaculture facilities so as to monitor growth performance and food intake. Food (de-frozen fish - bogue, mackerel and squid - or moist pellets) was delivered ad libitum. Total length and wet weight were measured regularly. Daily food intake, the food conversion index and daily growth rate were calculated. Individuals, aging 0+, grew exceptionally fast, reaching 2.0kg (total length 50cm) in 12 months and 5.0kg (total length 65cm) in 24, while at later stages, the growth rate decreased and depended on the water temperature during rearing. Food consumption varied during the rearing period according to the developmental stage and the rearing conditions. The food conversion ratio ranged between 0.9 and 2.5 on dry weight basis during the period of the study. Gametogenesis was monitored in a separate stock of 15 individuals (11.7 ± 3.7 kg) by biopsis while blood samples were taken for steroid estimation. Increased oocyte diameter was observed in females from August to January, while plasma estradiol levels also increased from October to March. However, neither egg or sperm were collected from the stock. Young wreckfish presented a high growth rate in captivity, thus supporting the feasibility of the species for rearing. Further research, however, is required for the reproduction of the species. © 2004 Kluwer Academic Publishers. |
Papandroulakis, N; Kentouri, M; Maingot, E; Divanach, P Mesocosm: A reliable technology for larval rearing of Diplodus puntazzo and Diplodus sargus sargus Journal Article Aquaculture International, 12 , pp. 345 – 355, 2004. @article{papandroulakis_mesocosm_2004, title = {Mesocosm: A reliable technology for larval rearing of Diplodus puntazzo and Diplodus sargus sargus}, author = {N Papandroulakis and M Kentouri and E Maingot and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33644987838&partnerID=40&md5=76b681983b3ee6f2281fdabaab3a4b3d}, year = {2004}, date = {2004-01-01}, journal = {Aquaculture International}, volume = {12}, pages = {345 -- 355}, abstract = {Species diversification is today considered as a major issue for the sustainable development of the Mediterranean aquaculture. For successful propagation of any species however, larval rearing is considered a bottleneck and therefore the development of appropriate tools is essential. Mesocosm is a semi-intensive technology that facilitates larval rearing of several species integrating principles of both intensive and extensive aquaculture, which solves biological problems and many of their technical, human and economical consequences. The extensive (and now even the semi-extensive) strategy is used in the most critical segments of the rearing process during the early developmental stages, when larvae are still extremely weak, sensitive to intensive environment, easily stressed and difficult to feed. The intensive strategy is used as soon as larvae are considered mature enough to be reared easily using classical methods. The technology was used for the rearing of two species, with potential for aquaculture, the sharpsnout seabream (Diplodus puntazzo) and the white seabream (Diplodus sargus sargus). Three groups of each species were monitored for a period of 50-70 days post hatching. Survival for both species was about 54% at the end of the trials. Sharpsnout seabream larvae reached 19.6 ± 0.9 mm total length and 107.2±31.9mg body weight 50 days post hatching. White seabream larvae 60 days post hatching reached 32.7 ± 2.7 mm total length and 450±70mg body weight. In order to verify the economical viability of the technology, the individual production cost for each species was estimated and reached €0.027 for white seabream and €0.043 for sharpsnout seabream. Results indicate the reliability of the technology for the larval rearing of the two species. © 2004 Kluwer Academic Publishers.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Species diversification is today considered as a major issue for the sustainable development of the Mediterranean aquaculture. For successful propagation of any species however, larval rearing is considered a bottleneck and therefore the development of appropriate tools is essential. Mesocosm is a semi-intensive technology that facilitates larval rearing of several species integrating principles of both intensive and extensive aquaculture, which solves biological problems and many of their technical, human and economical consequences. The extensive (and now even the semi-extensive) strategy is used in the most critical segments of the rearing process during the early developmental stages, when larvae are still extremely weak, sensitive to intensive environment, easily stressed and difficult to feed. The intensive strategy is used as soon as larvae are considered mature enough to be reared easily using classical methods. The technology was used for the rearing of two species, with potential for aquaculture, the sharpsnout seabream (Diplodus puntazzo) and the white seabream (Diplodus sargus sargus). Three groups of each species were monitored for a period of 50-70 days post hatching. Survival for both species was about 54% at the end of the trials. Sharpsnout seabream larvae reached 19.6 ± 0.9 mm total length and 107.2±31.9mg body weight 50 days post hatching. White seabream larvae 60 days post hatching reached 32.7 ± 2.7 mm total length and 450±70mg body weight. In order to verify the economical viability of the technology, the individual production cost for each species was estimated and reached €0.027 for white seabream and €0.043 for sharpsnout seabream. Results indicate the reliability of the technology for the larval rearing of the two species. © 2004 Kluwer Academic Publishers. |
2003 |
Sfakianakis, D G; Koumoundouros, G; Anezaki, L; Divanach, P; Kentouri, M Development of a saddleback-like syndrome in reared white seabream Diplodus sargus (Linnaeus, 1758) Journal Article Aquaculture, 217 (1-4), pp. 673–676, 2003, ISSN: 00448486. @article{sfakianakis_development_2003, title = {Development of a saddleback-like syndrome in reared white seabream Diplodus sargus (Linnaeus, 1758)}, author = {D G Sfakianakis and G Koumoundouros and L Anezaki and P Divanach and M Kentouri}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848602001990}, doi = {10.1016/S0044-8486(02)00199-0}, issn = {00448486}, year = {2003}, date = {2003-03-01}, urldate = {2020-08-17}, journal = {Aquaculture}, volume = {217}, number = {1-4}, pages = {673--676}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Rainis, S; Mylonas, C C; Kyriakou, Y; Divanach, P Enhancement of spermiation in European sea bass (Dicentrarchus labrax) at the end of the reproductive season using GnRHa implants Journal Article Aquaculture, 219 (1-4), pp. 873–890, 2003, ISSN: 00448486, (Publisher: Elsevier). @article{rainis_enhancement_2003, title = {Enhancement of spermiation in European sea bass (Dicentrarchus labrax) at the end of the reproductive season using GnRHa implants}, author = {S Rainis and C C Mylonas and Y Kyriakou and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037414059&doi=10.1016%2fS0044-8486%2803%2900028-0&partnerID=40&md5=1ef1061621cd17c6bab8bac850349250}, doi = {10.1016/S0044-8486(03)00028-0}, issn = {00448486}, year = {2003}, date = {2003-01-01}, journal = {Aquaculture}, volume = {219}, number = {1-4}, pages = {873--890}, abstract = {Spermiating European sea bass (Dicentrarchus labrax) were treated at the end of the reproductive period (22 February 2002) with implants of gonadotropin-releasing hormone agonist (GnRHa), in order to examine their potential in enhancing expressible milt volume, sperm quality and in vitro fertilization success. The GnRHa implants at all doses (20, 40 and 80 μg GnRHa kg-1 body weight [bw]) stimulated a significantly higher production of milt compared to controls over a 27-day period, increasing total volume from 0.5 to 2.2 ml kg-1 sample time-1. No differences in milt production were observed among the three GnRHa doses. Histological evaluation of the testes indicated that, by day 21, control fish had entered into gonadal regression, containing almost exclusively spermatogonia. At the same time, testes from GnRHa-implanted fish still contained large numbers of spermatozoa in the testes, indicating that spermiation was still underway. Sperm density varied between 7.4 and 14.4 × 1010 spermatozoa ml-1, but remained unchanged in response to GnRHa implantation. Sperm motility was high at the beginning of the experiment (92-100%) and decreased significantly by day 27 (32-55%), whereas motility duration was unchanged (0.9-1.2 min). Neither sperm motility nor motility duration was affected by the GnRHa treatment. Finally, fertilization success was high (40-80%) and not significantly different in response to GnRHa implantation. These results demonstrate that GnRHa implants are effective at increasing milt production at the end of the spermiation period, maintaining high sperm density and not affecting, either positively or negatively, sperm quality and fertilization capacity. © 2003 Elsevier Science B.V. All rights reserved.}, note = {Publisher: Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {article} } Spermiating European sea bass (Dicentrarchus labrax) were treated at the end of the reproductive period (22 February 2002) with implants of gonadotropin-releasing hormone agonist (GnRHa), in order to examine their potential in enhancing expressible milt volume, sperm quality and in vitro fertilization success. The GnRHa implants at all doses (20, 40 and 80 μg GnRHa kg-1 body weight [bw]) stimulated a significantly higher production of milt compared to controls over a 27-day period, increasing total volume from 0.5 to 2.2 ml kg-1 sample time-1. No differences in milt production were observed among the three GnRHa doses. Histological evaluation of the testes indicated that, by day 21, control fish had entered into gonadal regression, containing almost exclusively spermatogonia. At the same time, testes from GnRHa-implanted fish still contained large numbers of spermatozoa in the testes, indicating that spermiation was still underway. Sperm density varied between 7.4 and 14.4 × 1010 spermatozoa ml-1, but remained unchanged in response to GnRHa implantation. Sperm motility was high at the beginning of the experiment (92-100%) and decreased significantly by day 27 (32-55%), whereas motility duration was unchanged (0.9-1.2 min). Neither sperm motility nor motility duration was affected by the GnRHa treatment. Finally, fertilization success was high (40-80%) and not significantly different in response to GnRHa implantation. These results demonstrate that GnRHa implants are effective at increasing milt production at the end of the spermiation period, maintaining high sperm density and not affecting, either positively or negatively, sperm quality and fertilization capacity. © 2003 Elsevier Science B.V. All rights reserved. |
Mylonas, C C; Papadaki, M; Divanach, P Seasonal changes in sperm production and quality in the red porgy Pagrus pagrus (L.) Journal Article Aquaculture Research, 34 (13), pp. 1161–1170, 2003, ISSN: 1355557X. @article{mylonas_seasonal_2003, title = {Seasonal changes in sperm production and quality in the red porgy Pagrus pagrus (L.)}, author = {C C Mylonas and M Papadaki and P Divanach}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0242286610&doi=10.1046%2fj.1365-2109.2003.00922.x&partnerID=40&md5=035f37a6d19b84e81a7004fb96f03fec}, doi = {10.1046/j.1365-2109.2003.00922.x}, issn = {1355557X}, year = {2003}, date = {2003-01-01}, journal = {Aquaculture Research}, volume = {34}, number = {13}, pages = {1161--1170}, abstract = {Cultured red porgy Pagrus pagrus (L.) males (n = 6) were sampled every 2 weeks for milt, in order to monitor changes in sperm quality parameters during a whole spawning period. On 11 January 2001, 60% of the fish were spermiating, increasing to 100% in mid-February and dropping to 30% by mid-April. Sperm density showed a slight increasing trend, with mean values ranging between 8.6 and 23.7 × 109 spermatozoa mL-1. Sperm motility percentage exhibited a significant improvement during the spawning season (analysis of variance (ANOVA) P = 0.0001). The duration of forward motility for the major part of the monitoring period ranged between 2 and 4 min. Red porgy spermatozoa maintained their viability for many days after whole storage of milt at 4°C. During the monitoring period there were significant changes in the mean duration of sperm survival after cold storage, ranging from 5 to 12 days. The total volume of expressible milt was maximal on 28 March, increasing from a mean value of 1.7 mL to 5.3 mL kg-1. Milt production of captive-reared red porgy does not appear to be limiting, when compared with the volume of expressible milt produced by other cultured marine fishes.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Cultured red porgy Pagrus pagrus (L.) males (n = 6) were sampled every 2 weeks for milt, in order to monitor changes in sperm quality parameters during a whole spawning period. On 11 January 2001, 60% of the fish were spermiating, increasing to 100% in mid-February and dropping to 30% by mid-April. Sperm density showed a slight increasing trend, with mean values ranging between 8.6 and 23.7 × 109 spermatozoa mL-1. Sperm motility percentage exhibited a significant improvement during the spawning season (analysis of variance (ANOVA) P = 0.0001). The duration of forward motility for the major part of the monitoring period ranged between 2 and 4 min. Red porgy spermatozoa maintained their viability for many days after whole storage of milt at 4°C. During the monitoring period there were significant changes in the mean duration of sperm survival after cold storage, ranging from 5 to 12 days. The total volume of expressible milt was maximal on 28 March, increasing from a mean value of 1.7 mL to 5.3 mL kg-1. Milt production of captive-reared red porgy does not appear to be limiting, when compared with the volume of expressible milt produced by other cultured marine fishes. |
Mylonas, C C; Sigelaki, I; Divanach, P; Mananõs, E; Carrillo, M; Afonso-Polyviou, A Multiple spawning and egg quality of individual European sea bass (Dicentrarchus labrax) females after repeated injections of GnRHa Journal Article Aquaculture, 221 (1-4), pp. 605–620, 2003, ISSN: 00448486, (Publisher: Elsevier). @article{mylonas_multiple_2003, title = {Multiple spawning and egg quality of individual European sea bass (Dicentrarchus labrax) females after repeated injections of GnRHa}, author = {C C Mylonas and I Sigelaki and P Divanach and E Mananõs and M Carrillo and A Afonso-Polyviou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037402489&doi=10.1016%2fS0044-8486%2803%2900120-0&partnerID=40&md5=4fa3ca75d178e099a929643bd3f005cd}, doi = {10.1016/S0044-8486(03)00120-0}, issn = {00448486}, year = {2003}, date = {2003-01-01}, journal = {Aquaculture}, volume = {221}, number = {1-4}, pages = {605--620}, abstract = {Using multiple injections of gonadotropin-releasing hormone agonist (GnRHa) (10 μg kg-1 bw) spaced 7-14 days apart, we investigated the multiple-batch group-synchronous spawning kinetics of individual European sea bass females (n = 6) and examined batch fecundity and egg quality parameters. Spawning was obtained 3-4 days after each GnRHa injection. A mean of 2.8 spawns female-1 was obtained, with a minimum of 2 and maximum of 4. Mean relative fecundity of the first spawn was 320,211 eggs kg-1 bw and decreased continuously in subsequent spawns, reaching 52,381 eggs kg-1. Total mean relative fecundity for all spawns was 637,900 eggs kg-1. Mean fertilization success did not vary significantly among sequential spawns and ranged between 67% and 91%. On the contrary, there were significant differences among the four spawns in embryo survival 1 day after egg collection, hatching percentage and larval survival 4 days after egg collection, but there was no consistent trend during the study. Significant positive correlations existed between 4-day larval survival and hatching percentage (P=0.0002), and between hatching percentage and embryo survival 1 day after egg collection (P=0.03), indicating that 1-day embryo survival can be a potential biomarker for egg quality in commercial hatcheries. The results of the study demonstrate that European sea bass can produce up to four sequential spawns of high quality eggs in response to repeated acute treatments of GnRHa, and suggest that a pulsatile treatment of GnRHa may be necessary to induce the appropriate cycles of hormone changes for the recruitment, maturation and ovulation of multiple batches of viable eggs. © 2003 Elsevier Science B.V. All rights reserved.}, note = {Publisher: Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {article} } Using multiple injections of gonadotropin-releasing hormone agonist (GnRHa) (10 μg kg-1 bw) spaced 7-14 days apart, we investigated the multiple-batch group-synchronous spawning kinetics of individual European sea bass females (n = 6) and examined batch fecundity and egg quality parameters. Spawning was obtained 3-4 days after each GnRHa injection. A mean of 2.8 spawns female-1 was obtained, with a minimum of 2 and maximum of 4. Mean relative fecundity of the first spawn was 320,211 eggs kg-1 bw and decreased continuously in subsequent spawns, reaching 52,381 eggs kg-1. Total mean relative fecundity for all spawns was 637,900 eggs kg-1. Mean fertilization success did not vary significantly among sequential spawns and ranged between 67% and 91%. On the contrary, there were significant differences among the four spawns in embryo survival 1 day after egg collection, hatching percentage and larval survival 4 days after egg collection, but there was no consistent trend during the study. Significant positive correlations existed between 4-day larval survival and hatching percentage (P=0.0002), and between hatching percentage and embryo survival 1 day after egg collection (P=0.03), indicating that 1-day embryo survival can be a potential biomarker for egg quality in commercial hatcheries. The results of the study demonstrate that European sea bass can produce up to four sequential spawns of high quality eggs in response to repeated acute treatments of GnRHa, and suggest that a pulsatile treatment of GnRHa may be necessary to induce the appropriate cycles of hormone changes for the recruitment, maturation and ovulation of multiple batches of viable eggs. © 2003 Elsevier Science B.V. All rights reserved. |
Mylonas, C C; Anezaki, L; Divanach, P; Zanuy, S; Piferrer, F; Ron, B; Peduel, A; Atia, Ben I; Gorshkov, S; Tandler, A Influence of rearing temperature at two periods during early life on growth and sex differentiation of two strains of European sea bass Journal Article Fish Physiology and Biochemistry, 28 (1-4), pp. 167–168, 2003, ISSN: 09201742. @article{mylonas_influence_2003, title = {Influence of rearing temperature at two periods during early life on growth and sex differentiation of two strains of European sea bass}, author = {C C Mylonas and L Anezaki and P Divanach and S Zanuy and F Piferrer and B Ron and A Peduel and I Ben Atia and S Gorshkov and A Tandler}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-8844247073&doi=10.1023%2fB%3aFISH.0000030514.63577.84&partnerID=40&md5=14276c977f32f39ea79feff0f756a6c1}, doi = {10.1023/B:FISH.0000030514.63577.84}, issn = {09201742}, year = {2003}, date = {2003-01-01}, journal = {Fish Physiology and Biochemistry}, volume = {28}, number = {1-4}, pages = {167--168}, abstract = {The effect of water temperature on sex differentiation was examined in European sea bass (Dicentrarchus labrax) of two strains from the Northwestern and Southeastern Mediterranean Sea, during larval or nursery rearing. Temperatures textless 17 °C enhanced feminization, while 21 °C during larval rearing induced higher male% compared to the nursery stage, and the overall male% was significantly different among strains (Ptextless0.05). The study shows that management of larval rearing temperature can prevent the high male% observed in cultured populations. © 2004 Kluwer Academic Publishers.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The effect of water temperature on sex differentiation was examined in European sea bass (Dicentrarchus labrax) of two strains from the Northwestern and Southeastern Mediterranean Sea, during larval or nursery rearing. Temperatures textless 17 °C enhanced feminization, while 21 °C during larval rearing induced higher male% compared to the nursery stage, and the overall male% was significantly different among strains (Ptextless0.05). The study shows that management of larval rearing temperature can prevent the high male% observed in cultured populations. © 2004 Kluwer Academic Publishers. |
Pavlidis, M; Angellotti, L; Papandroulakis, N; Divanach, P Evaluation of transportation procedures on water quality and fry performance in red porgy (Pagrus pagrus) fry Journal Article Aquaculture, 218 , pp. 187 – 202, 2003. @article{pavlidis_evaluation_2003, title = {Evaluation of transportation procedures on water quality and fry performance in red porgy (Pagrus pagrus) fry}, author = {M Pavlidis and L Angellotti and N Papandroulakis and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0037468983&partnerID=40&md5=80ecd722fd5c0e27d85db4c0de5ae08f}, year = {2003}, date = {2003-01-01}, journal = {Aquaculture}, volume = {218}, pages = {187 -- 202}, abstract = {The effect of stocking density (10, 20, 30, 40 kg/m3), water renewal (0% or 100% renewal rate per hour), hauling temperature (14, 19, 24 °C), salinity (20, 25, 30, 35, 40 psu), and use of anaesthesia (0, 10, 20, or 50 ppm ethynelglycol-monophenylether) prior to transportation on red porgy’s fry performance, liver glycogen, and water quality parameters was tested to evaluate transportation procedures in a promising candidate for aquaculture. Simulated transport was performed in plastic containers (volume 60 1) for 48 h. Water samples were taken at 4-h intervals after transport for the determination of pH, carbon dioxide (CO2), un-ionised ammonia (NH3) and ammonium (NH4 +). Additionally, liver samples were collected at 4 and 48 h for glycogen determination. There were no statistically significant fluctuations in dissolved CO2 concentration in all tested conditions. Stocking density did not affect NH3 and NH4 + average values and hepatic glycogen content in groups exposed to a water renewal rate of 100%, while increasing NH3 and NH4 + average values with increasing stocking density was observed in groups with no water renewal. Under the same stocking density, a significant change in NH3 and NH4 + fluctuations over the duration of the experiment was observed with concentrations increasing, with a mean exponential rate (± SD) of 0.060 ± 0.005 (NH3) and 0.062 ± 0.005 (NH4 +) per hour in groups with no water renewal, and -0.033 ± 0.004 (NH3) and -0.024 ± 0.007 (NH4 +) per hour in groups with 100% water renewal. Water temperature affected significantly the hepatic glycogen content and survival during transport. There was no significant effect of salinity and anaesthetic (except at a dose of 50 ppm) on fry survival and on the water quality parameters. It is suggested that red porgy should be transported in stocking densities of 20-25 kg/m3 and at a hauling temperature similar to that kept at the exporter’s fish rearing tanks (preferable 19 °C). Besides, it is recommended to avoid temperature differences between the hauling water and the water used for renewal during fry transportation. © 2003 Elsevier Science B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The effect of stocking density (10, 20, 30, 40 kg/m3), water renewal (0% or 100% renewal rate per hour), hauling temperature (14, 19, 24 °C), salinity (20, 25, 30, 35, 40 psu), and use of anaesthesia (0, 10, 20, or 50 ppm ethynelglycol-monophenylether) prior to transportation on red porgy’s fry performance, liver glycogen, and water quality parameters was tested to evaluate transportation procedures in a promising candidate for aquaculture. Simulated transport was performed in plastic containers (volume 60 1) for 48 h. Water samples were taken at 4-h intervals after transport for the determination of pH, carbon dioxide (CO2), un-ionised ammonia (NH3) and ammonium (NH4 +). Additionally, liver samples were collected at 4 and 48 h for glycogen determination. There were no statistically significant fluctuations in dissolved CO2 concentration in all tested conditions. Stocking density did not affect NH3 and NH4 + average values and hepatic glycogen content in groups exposed to a water renewal rate of 100%, while increasing NH3 and NH4 + average values with increasing stocking density was observed in groups with no water renewal. Under the same stocking density, a significant change in NH3 and NH4 + fluctuations over the duration of the experiment was observed with concentrations increasing, with a mean exponential rate (± SD) of 0.060 ± 0.005 (NH3) and 0.062 ± 0.005 (NH4 +) per hour in groups with no water renewal, and -0.033 ± 0.004 (NH3) and -0.024 ± 0.007 (NH4 +) per hour in groups with 100% water renewal. Water temperature affected significantly the hepatic glycogen content and survival during transport. There was no significant effect of salinity and anaesthetic (except at a dose of 50 ppm) on fry survival and on the water quality parameters. It is suggested that red porgy should be transported in stocking densities of 20-25 kg/m3 and at a hauling temperature similar to that kept at the exporter’s fish rearing tanks (preferable 19 °C). Besides, it is recommended to avoid temperature differences between the hauling water and the water used for renewal during fry transportation. © 2003 Elsevier Science B.V. All rights reserved. |
Machias, A; Somarakis, S; Papandroulakis, N; Spedicato, M -T; Suquet, M; Lembo, G; Divanach, P Settlement of the wreckfish (Polyprion americanus) Journal Article Marine Biology, 142 (1), pp. 45–52, 2003, ISSN: 0025-3162. @article{machias_settlement_2003, title = {Settlement of the wreckfish (Polyprion americanus)}, author = {A Machias and S Somarakis and N Papandroulakis and M -T Spedicato and M Suquet and G Lembo and P Divanach}, url = {http://link.springer.com/10.1007/s00227-002-0918-2}, doi = {10.1007/s00227-002-0918-2}, issn = {0025-3162}, year = {2003}, date = {2003-01-01}, urldate = {2020-08-18}, journal = {Marine Biology}, volume = {142}, number = {1}, pages = {45--52}, abstract = {The wreckfish (Polyprion americanus) is a panoceanic, long-lived, deep-water demersal species, characterized by an extended pelagic juvenile phase associated with floating objects. In the present study morphometric data from 435 specimens collected from Greece (Crete), Italy (Ionian Sea) and the Atlantic coast of France from September 1999 to March 2001 were analyzed to estimate the settlement size of the species and associated changes in morphology. The fishery sample included specimens from both the pelagic and the demersal stage. Length-at-settlement (TL50) and 95% confidence intervals were estimated by fitting a logistic function. Eleven morphometric characters were analyzed, and the existing inflection points, reflecting alterations in body shape, were defined: (1) iteratively, as the transition point, splitting the data set into two groups, for which the reduced major axis functions, between a character and total length, of the successive groups best fit (minimum sum of squares of residuals) the combined data set and (2) where the second derivative of the fitted third-order polynomial functions to morphometric ratios equaled zero. The main size range within which wreckfish settled was 56-65 cm, and the means of the inflection points defined by the two methods were 61 and 64 cm. These results indicate changes in functional morphology associated with settlement. The monitoring of wreckfish juveniles caught in the wild and kept individually in captivity showed changes in growth and food intake at approximately the estimated settlement length and suggested that temperature was the most likely factor triggering settlement.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The wreckfish (Polyprion americanus) is a panoceanic, long-lived, deep-water demersal species, characterized by an extended pelagic juvenile phase associated with floating objects. In the present study morphometric data from 435 specimens collected from Greece (Crete), Italy (Ionian Sea) and the Atlantic coast of France from September 1999 to March 2001 were analyzed to estimate the settlement size of the species and associated changes in morphology. The fishery sample included specimens from both the pelagic and the demersal stage. Length-at-settlement (TL50) and 95% confidence intervals were estimated by fitting a logistic function. Eleven morphometric characters were analyzed, and the existing inflection points, reflecting alterations in body shape, were defined: (1) iteratively, as the transition point, splitting the data set into two groups, for which the reduced major axis functions, between a character and total length, of the successive groups best fit (minimum sum of squares of residuals) the combined data set and (2) where the second derivative of the fitted third-order polynomial functions to morphometric ratios equaled zero. The main size range within which wreckfish settled was 56-65 cm, and the means of the inflection points defined by the two methods were 61 and 64 cm. These results indicate changes in functional morphology associated with settlement. The monitoring of wreckfish juveniles caught in the wild and kept individually in captivity showed changes in growth and food intake at approximately the estimated settlement length and suggested that temperature was the most likely factor triggering settlement. |
2002 |
Koumoundouros, G; Maingot, E; Divanach, P; Kentouri, M Kyphosis in reared sea bass (Dicentrarchus labrax L.): ontogeny and effects on mortality Journal Article Aquaculture, 209 (1-4), pp. 49–58, 2002, ISSN: 00448486. @article{koumoundouros_kyphosis_2002, title = {Kyphosis in reared sea bass (Dicentrarchus labrax L.): ontogeny and effects on mortality}, author = {G Koumoundouros and E Maingot and P Divanach and M Kentouri}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848601008213}, doi = {10.1016/S0044-8486(01)00821-3}, issn = {00448486}, year = {2002}, date = {2002-06-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {209}, number = {1-4}, pages = {49--58}, abstract = {The ontogeny and the effects of vertebral kyphosis on the mortality rate of reared sea bass (Dicentrarchus labrax) are described. The first evidence of deformities was observed in individuals of 10 mm total length (TL) in the form of light malformations or bilateral asymmetries of individual vertebrae. At 14 mm mean TL, the 18.2% of the reared fish presented severe vertebral kyphosis at the pre-haemal region. Kyphosis continued to develop and presented its maximum incidence at 17 mm TL (24.8%). The centre of the deformity was mainly located on vertebrae 5-6. Kyphotic specimens presented a lethargic behaviour. After 17 mm mean TL, the incidence of the deformity decreased exponentially over time due to the heavy mortality of the affected fish (24.8% kyphosis incidence in the surviving fish vs. 52.6% in the dead fish collected from the tank, p textless 0.01). For the first time, it was shown that although a skeletal deformity could have little effect on the final quality of the reared juveniles, it can significantly affect the mortality rate and thus the productivity of the hatcheries. The results are discussed with respect to the aetiology, applications and research targets for the erasure of skeletal malformations from the reared fish. © 2002 Elsevier Science B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The ontogeny and the effects of vertebral kyphosis on the mortality rate of reared sea bass (Dicentrarchus labrax) are described. The first evidence of deformities was observed in individuals of 10 mm total length (TL) in the form of light malformations or bilateral asymmetries of individual vertebrae. At 14 mm mean TL, the 18.2% of the reared fish presented severe vertebral kyphosis at the pre-haemal region. Kyphosis continued to develop and presented its maximum incidence at 17 mm TL (24.8%). The centre of the deformity was mainly located on vertebrae 5-6. Kyphotic specimens presented a lethargic behaviour. After 17 mm mean TL, the incidence of the deformity decreased exponentially over time due to the heavy mortality of the affected fish (24.8% kyphosis incidence in the surviving fish vs. 52.6% in the dead fish collected from the tank, p textless 0.01). For the first time, it was shown that although a skeletal deformity could have little effect on the final quality of the reared juveniles, it can significantly affect the mortality rate and thus the productivity of the hatcheries. The results are discussed with respect to the aetiology, applications and research targets for the erasure of skeletal malformations from the reared fish. © 2002 Elsevier Science B.V. All rights reserved. |
Koumoundouros, George; Pavlidis, Michalis; Anezaki, Lina; Kokkari, Constantina; Sterioti, Aspasia; Divanach, Pascal; Kentouri, Maroudio Journal of Experimental Zoology, 292 (6), pp. 573–579, 2002, ISSN: 0022-104X, 1097-010X. @article{koumoundouros_temperature_2002, title = {Temperature sex determination in the European sea bass, Dicentrarchus labrax (L., 1758) (Teleostei, Perciformes, Moronidae): Critical sensitive ontogenetic phase}, author = {George Koumoundouros and Michalis Pavlidis and Lina Anezaki and Constantina Kokkari and Aspasia Sterioti and Pascal Divanach and Maroudio Kentouri}, url = {http://doi.wiley.com/10.1002/jez.10095}, doi = {10.1002/jez.10095}, issn = {0022-104X, 1097-010X}, year = {2002}, date = {2002-05-01}, urldate = {2020-08-18}, journal = {Journal of Experimental Zoology}, volume = {292}, number = {6}, pages = {573--579}, abstract = {The temperature sex determination (TSD) mechanism in the European sea bass (Dicentrarchus labrax L.) was studied in respect to: a) the TSD sensitivity during the different developmental stages; and b) the intrapopulation correlation of sex determination with the growth rate up to the end of the TSD-sensitive period. At the stage of half-epiboly, eggs from the same batch were divided into four groups and subjected to different thermal treatments: a) 15°C (G15 group) and b) 20°C (G20 group) up to the middle of metamorphosis stage; c) 15°C up to the end of yolk-sac larval stage and subsequently to 20°C (G15-5 group); and d) 15°C up to the end of the preflexion stage and then to 20°C (G15-10 group). At the end of the treatments, size grading was applied and four additional populations were established from the upper (L) and lower (S) size portions of the G15 and G20 populations: G15L, G15S, G20L, and G20S. During the following growing phase, all populations were subjected to common rearing conditions. The sex ratios of each population were macroscopically determined at 190-210 mm mean total length. Female incidence was significantly affected (P textless 0.05) by the different thermal treatments: 66.1% in the G15, 47.1% in the G15-10, 37.6% in the G15-5, and 18.1% in the G20 group. In addition, sex ratio was correlated with the growth rate of the fish up to the end of the TSD-sensitive period, with the larger fish presenting a significantly higher (P textless 0.01) female incidence than the smaller fish in both thermal regimes tested: 73.1% in G15L vs. 57% in G15S, and 36.6%in G20L vs. 22.5% in G20S group. Results provide, for the first time, clear evidence that the sea bass is sensitive to TSD during all different ontogenetic stages up to metamorphosis, and that sex ratio is correlated with the growth rate of the fish well before the differentiation and maturation of the gonads. © 2002 Wiley-Liss, Inc.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The temperature sex determination (TSD) mechanism in the European sea bass (Dicentrarchus labrax L.) was studied in respect to: a) the TSD sensitivity during the different developmental stages; and b) the intrapopulation correlation of sex determination with the growth rate up to the end of the TSD-sensitive period. At the stage of half-epiboly, eggs from the same batch were divided into four groups and subjected to different thermal treatments: a) 15°C (G15 group) and b) 20°C (G20 group) up to the middle of metamorphosis stage; c) 15°C up to the end of yolk-sac larval stage and subsequently to 20°C (G15-5 group); and d) 15°C up to the end of the preflexion stage and then to 20°C (G15-10 group). At the end of the treatments, size grading was applied and four additional populations were established from the upper (L) and lower (S) size portions of the G15 and G20 populations: G15L, G15S, G20L, and G20S. During the following growing phase, all populations were subjected to common rearing conditions. The sex ratios of each population were macroscopically determined at 190-210 mm mean total length. Female incidence was significantly affected (P textless 0.05) by the different thermal treatments: 66.1% in the G15, 47.1% in the G15-10, 37.6% in the G15-5, and 18.1% in the G20 group. In addition, sex ratio was correlated with the growth rate of the fish up to the end of the TSD-sensitive period, with the larger fish presenting a significantly higher (P textless 0.01) female incidence than the smaller fish in both thermal regimes tested: 73.1% in G15L vs. 57% in G15S, and 36.6%in G20L vs. 22.5% in G20S group. Results provide, for the first time, clear evidence that the sea bass is sensitive to TSD during all different ontogenetic stages up to metamorphosis, and that sex ratio is correlated with the growth rate of the fish well before the differentiation and maturation of the gonads. © 2002 Wiley-Liss, Inc. |
Koumoundouros, G; Sfakianakis, D G; Divanach, P; Kentouri, M Effect of temperature on swimming performance of sea bass juveniles Journal Article Journal of Fish Biology, 60 (4), pp. 923–932, 2002, ISSN: 0022-1112, 1095-8649. @article{koumoundouros_effect_2002, title = {Effect of temperature on swimming performance of sea bass juveniles}, author = {G Koumoundouros and D G Sfakianakis and P Divanach and M Kentouri}, url = {http://doi.wiley.com/10.1111/j.1095-8649.2002.tb02418.x}, doi = {10.1111/j.1095-8649.2002.tb02418.x}, issn = {0022-1112, 1095-8649}, year = {2002}, date = {2002-04-01}, urldate = {2020-08-18}, journal = {Journal of Fish Biology}, volume = {60}, number = {4}, pages = {923--932}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Papandroulakis, Nikos; Papaioannou, Dimitris; Divanach, Pascal An automated feeding system for intensive hatcheries Journal Article Aquacultural Engineering, 26 (1), pp. 13–26, 2002, ISSN: 01448609. @article{papandroulakis_automated_2002, title = {An automated feeding system for intensive hatcheries}, author = {Nikos Papandroulakis and Dimitris Papaioannou and Pascal Divanach}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0144860901000917}, doi = {10.1016/S0144-8609(01)00091-7}, issn = {01448609}, year = {2002}, date = {2002-03-01}, urldate = {2020-08-06}, journal = {Aquacultural Engineering}, volume = {26}, number = {1}, pages = {13--26}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Papandroulakis, N; Divanach, P; Kentouri, M Enhanced biological performance of intensive sea bream (Sparus aurata) larviculture in the presence of phytoplankton with long photophase Journal Article Aquaculture, 204 , pp. 45 – 63, 2002. @article{papandroulakis_enhanced_2002, title = {Enhanced biological performance of intensive sea bream (Sparus aurata) larviculture in the presence of phytoplankton with long photophase}, author = {N Papandroulakis and P Divanach and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0037148117&partnerID=40&md5=05a492b10b2b9875a20edf9b69f585b8}, year = {2002}, date = {2002-01-01}, journal = {Aquaculture}, volume = {204}, pages = {45 -- 63}, abstract = {Sea bream larvae were reared under intensive conditions either with (pseudo-green water technique) or without (clear water technique) the addition of phytoplankton in the rearing tanks, under 24- or 18-h photophases. Phytoplankton presence in the rearing medium resulted in 44 ± 17% survival and individuals of 2.0 ± 0.2 mg wet weight after 20 days of rearing. With the clear water method, both survival and growth decreased to 16 ± 6% and 1.1 ± 0.2 mg, respectively. The daily consumption rate (as milligram of carbon consumed per milligram body carbon) at the beginning of exogenous feeding was 0.5 day-1 for all experimental conditions. At later stages, individuals reared with phytoplankton present had a daily consumption rate of about 0.7 day-1, while those reared with the clear water technique presented consumption rates from 1.0 (for 24-h photophase) to 1.9 day-1 (for 18-h photophase). The mean food assimilation efficiency of the larvae, expressed as a Food Conversion Index (FCI), was between 6.3 ± 1.4 (for 18-h photophase) and 8.2 ± 1.2 (for 24-h photophase) for the individuals reared in the presence of phytoplankton. For larvae reared using the clear water technique, FCI was between 12.8 ± 2.4 (for 24-h photophase) and 20.1 ± 5.3 (for 18-h photophase). © 2002 Elsevier Science B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Sea bream larvae were reared under intensive conditions either with (pseudo-green water technique) or without (clear water technique) the addition of phytoplankton in the rearing tanks, under 24- or 18-h photophases. Phytoplankton presence in the rearing medium resulted in 44 ± 17% survival and individuals of 2.0 ± 0.2 mg wet weight after 20 days of rearing. With the clear water method, both survival and growth decreased to 16 ± 6% and 1.1 ± 0.2 mg, respectively. The daily consumption rate (as milligram of carbon consumed per milligram body carbon) at the beginning of exogenous feeding was 0.5 day-1 for all experimental conditions. At later stages, individuals reared with phytoplankton present had a daily consumption rate of about 0.7 day-1, while those reared with the clear water technique presented consumption rates from 1.0 (for 24-h photophase) to 1.9 day-1 (for 18-h photophase). The mean food assimilation efficiency of the larvae, expressed as a Food Conversion Index (FCI), was between 6.3 ± 1.4 (for 18-h photophase) and 8.2 ± 1.2 (for 24-h photophase) for the individuals reared in the presence of phytoplankton. For larvae reared using the clear water technique, FCI was between 12.8 ± 2.4 (for 24-h photophase) and 20.1 ± 5.3 (for 18-h photophase). © 2002 Elsevier Science B.V. All rights reserved. |
Machias, Athanassios; Maraveyia, Eleni; Pavlidis, Michalis; Somarakis, Stylianos; Divanach, Pascal Validation of annuli on scales and otoliths of common dentex (Dentex dentex) Journal Article Fisheries Research, 54 (2), pp. 287–294, 2002, ISSN: 01657836. @article{machias_validation_2002, title = {Validation of annuli on scales and otoliths of common dentex (Dentex dentex)}, author = {Athanassios Machias and Eleni Maraveyia and Michalis Pavlidis and Stylianos Somarakis and Pascal Divanach}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0165783600003027}, doi = {10.1016/S0165-7836(00)00302-7}, issn = {01657836}, year = {2002}, date = {2002-01-01}, urldate = {2020-08-18}, journal = {Fisheries Research}, volume = {54}, number = {2}, pages = {287--294}, abstract = {The formation of annuli was examined from scales and otoliths of farmed (known age) common dentex, Dentex dentex. One scale annulus formed each year during May and one hyaline zone on the otoliths during June. The formation of scale annuli and the hyaline zone on otoliths of adult fish coincided with gonadal maturation which takes place during April to May. Scales were found to be more accurate than otoliths in respect to annuli formation, but their annuli were not as intense and distinct. Missing or additional hyaline zones were observed on adult otoliths. These irregularities were observed after fish maturation and were related to temperature conditions, as well as to faster growth of the farmed fish than the wild ones. © 2002 Elsevier Science B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The formation of annuli was examined from scales and otoliths of farmed (known age) common dentex, Dentex dentex. One scale annulus formed each year during May and one hyaline zone on the otoliths during June. The formation of scale annuli and the hyaline zone on otoliths of adult fish coincided with gonadal maturation which takes place during April to May. Scales were found to be more accurate than otoliths in respect to annuli formation, but their annuli were not as intense and distinct. Missing or additional hyaline zones were observed on adult otoliths. These irregularities were observed after fish maturation and were related to temperature conditions, as well as to faster growth of the farmed fish than the wild ones. © 2002 Elsevier Science B.V. All rights reserved. |
2001 |
Koumoundouros, G; Divanach, P; Anezaki, L; Kentouri, M Temperature-induced ontogenetic plasticity in sea bass (Dicentrarchus labrax) Journal Article Marine Biology, 139 (5), pp. 817 – 830, 2001, ISSN: 0025-3162, 1432-1793. @article{koumoundouros_temperature-induced_2001, title = {Temperature-induced ontogenetic plasticity in sea bass (Dicentrarchus labrax)}, author = {G Koumoundouros and P Divanach and L Anezaki and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035651680&partnerID=40&md5=c0b2c440d241826ebd72b40d6ade80f2}, doi = {10.1007/s002270100635}, issn = {0025-3162, 1432-1793}, year = {2001}, date = {2001-11-01}, journal = {Marine Biology}, volume = {139}, number = {5}, pages = {817 -- 830}, abstract = {We studied the ontogeny of Dicentrarchus labrax comparatively under constant rearing temperatures of 13, 15 and 20°C. At hatching, yolk-sac larval morphometry differed significantly between the temperatures and especially between the two extremes, while at the end of the yolk-sac larval stage, it mainly differed between the two lower temperature regimes and that of 20°C. Compared with the two lower temperature conditions, at 20°C D. labrax presented a significant ontogenetic acceleration which was morphologically expressed either as a significantly smaller total length (TL) at feeding onset, notochord flexion and fin differentiation, or as shifts of the allometric inflection points of 8 out of the 15 morphometric characters studied. Additionally, temperature significantly affected the allometry coefficients, with a decreasing growth intensity as the temperature difference decreased. The rate of TL growth increased under elevated temperature conditions throughout the entire ontogenetic period, except during the early larval period (feeding onset to metamorphosis onset), at which time D. labrax presented equal growth rates at 15°C and 20°C. The results are discussed with respect to the ontogeny of the functional morphology and the meanings of temperature- induced ontogenetic plasticity for the survival of fish larvae.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We studied the ontogeny of Dicentrarchus labrax comparatively under constant rearing temperatures of 13, 15 and 20°C. At hatching, yolk-sac larval morphometry differed significantly between the temperatures and especially between the two extremes, while at the end of the yolk-sac larval stage, it mainly differed between the two lower temperature regimes and that of 20°C. Compared with the two lower temperature conditions, at 20°C D. labrax presented a significant ontogenetic acceleration which was morphologically expressed either as a significantly smaller total length (TL) at feeding onset, notochord flexion and fin differentiation, or as shifts of the allometric inflection points of 8 out of the 15 morphometric characters studied. Additionally, temperature significantly affected the allometry coefficients, with a decreasing growth intensity as the temperature difference decreased. The rate of TL growth increased under elevated temperature conditions throughout the entire ontogenetic period, except during the early larval period (feeding onset to metamorphosis onset), at which time D. labrax presented equal growth rates at 15°C and 20°C. The results are discussed with respect to the ontogeny of the functional morphology and the meanings of temperature- induced ontogenetic plasticity for the survival of fish larvae. |
Koumoundouros, G; Sfakianakis, D; Maingot, E; Divanach, P; Kentouri, M Osteological development of the vertebral column and of the fins in Diplodus sargus (Teleostei: Perciformes: Sparidae) Journal Article Marine Biology, 139 (5), pp. 853 – 862, 2001, ISSN: 0025-3162, 1432-1793. @article{koumoundouros_osteological_2001, title = {Osteological development of the vertebral column and of the fins in Diplodus sargus (Teleostei: Perciformes: Sparidae)}, author = {G Koumoundouros and D Sfakianakis and E Maingot and P Divanach and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035654760&partnerID=40&md5=cc6c9efa1262f94c00bbb1f34b0fc21d}, doi = {10.1007/s002270100645}, issn = {0025-3162, 1432-1793}, year = {2001}, date = {2001-11-01}, journal = {Marine Biology}, volume = {139}, number = {5}, pages = {853 -- 862}, abstract = {The osteological development of vertebral column and fins in white sea bream, Diplodus sargus (Linnaeus, 1758) was studied. Vertebral ontogeny started at 5.7 mm total length (TL, post-fixation), with the formation of the first cartilaginous neural and haemal arches, and was completed by the full attainment of dorsal ribs (20.8 mm TL). The formation of vertebral centra occurred between 7.3 and 9.4 mm TL, but was fully completed only after the development of post- and pre-zygapophyses (26.0 mm TL). Pectoral supports were the first fin elements that started to develop (3.8 mm TL), followed by those of the caudal fin (5.2 mm TL), dorsal and anal fins (6.7 mm TL), and then by those of the pelvic fin (9.5 mm TL). The caudal fin of D. sargus was the first to develop fin rays and attain the full count of lepidotrichia (5.6-7.8 mm TL), but the last to attain the full count of dermatotrichia (7.9-16.0 mm TL). The next fins starting to present rays were the pectoral (6.5 mm TL) and the dorsal and anal fins (7.5 mm TL), while the pelvic fins were the last (11.5 mm TL). Following the caudal lepidotrichia (7.8 mm TL), the anal (10.5 mm TL), dorsal (12.0 mm TL), pectoral (11.4 mm TL) and pelvic fins (12.5 mm TL) were the next with fully completed ray counts. Finally, fin meristics were fully developed with the caudal dermatotrichia. The results are discussed in comparison with the fin and vertebral ontogeny of other teleosts.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The osteological development of vertebral column and fins in white sea bream, Diplodus sargus (Linnaeus, 1758) was studied. Vertebral ontogeny started at 5.7 mm total length (TL, post-fixation), with the formation of the first cartilaginous neural and haemal arches, and was completed by the full attainment of dorsal ribs (20.8 mm TL). The formation of vertebral centra occurred between 7.3 and 9.4 mm TL, but was fully completed only after the development of post- and pre-zygapophyses (26.0 mm TL). Pectoral supports were the first fin elements that started to develop (3.8 mm TL), followed by those of the caudal fin (5.2 mm TL), dorsal and anal fins (6.7 mm TL), and then by those of the pelvic fin (9.5 mm TL). The caudal fin of D. sargus was the first to develop fin rays and attain the full count of lepidotrichia (5.6-7.8 mm TL), but the last to attain the full count of dermatotrichia (7.9-16.0 mm TL). The next fins starting to present rays were the pectoral (6.5 mm TL) and the dorsal and anal fins (7.5 mm TL), while the pelvic fins were the last (11.5 mm TL). Following the caudal lepidotrichia (7.8 mm TL), the anal (10.5 mm TL), dorsal (12.0 mm TL), pectoral (11.4 mm TL) and pelvic fins (12.5 mm TL) were the next with fully completed ray counts. Finally, fin meristics were fully developed with the caudal dermatotrichia. The results are discussed in comparison with the fin and vertebral ontogeny of other teleosts. |
Koumoundouros, G; Divanach, P; Kentouri, M The effect of rearing conditions on development of saddleback syndrome and caudal fin deformities in Dentex dentex (L.) Journal Article Aquaculture, 200 (3-4), pp. 285–304, 2001, ISSN: 00448486. @article{koumoundouros_effect_2001, title = {The effect of rearing conditions on development of saddleback syndrome and caudal fin deformities in Dentex dentex (L.)}, author = {G Koumoundouros and P Divanach and M Kentouri}, url = {https://linkinghub.elsevier.com/retrieve/pii/S004484860100552X}, doi = {10.1016/S0044-8486(01)00552-X}, issn = {00448486}, year = {2001}, date = {2001-09-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {200}, number = {3-4}, pages = {285--304}, abstract = {The development of saddleback syndrome and of caudal fin deformities in Dentex dentex (Linnaeus, 1758) was compared under two rearing methods, extensive (E) and semi-extensive (S). The osteological appearance and the meristic characters of the reared fish were compared to that of D. dentex juveniles, collected from the natural environment. All the wild juveniles were normal in respect to their osteological appearance, while the reared specimens presented skeletal deformities resulting from different rearing methods applied. Saddleback syndrome (4.0-4.4%) and severe external deformities of the caudal fin (14.3-15.0%) characterised exclusively the semi-extensive populations, while the extensive populations presented severe abnormalities of the pre-ural centra (25.0-25.6%) with significantly higher frequency than the S reared fish (5.8%). Saddleback syndrome was expressed as a lack of one to all the hard spines of the dorsal fin, accompanied by shape, number and position abnormalities of the related pterygiophores. Caudal fin deformities were mainly characterised by the lack of the upper lepidotrichia or/and dermatotrichia, accompanied by severe deformities of the supporting elements of the upper lobe. The saddleback syndrome and severe caudal fin deformities were anatomically and ontogenetically related to each other, originating at the early larval stage as a result of abnormalities of the primordial marginal finfold and of the posterior tip of the notochord. In respect to the dominant phenotypes, the meristic characters of the normal reared fish were not differentiated from those of the wild, but they presented higher variability. The results are discussed in view of the aetiology, applications and research targets for the erasure of skeletal malformations. © 2001 Elsevier Science B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The development of saddleback syndrome and of caudal fin deformities in Dentex dentex (Linnaeus, 1758) was compared under two rearing methods, extensive (E) and semi-extensive (S). The osteological appearance and the meristic characters of the reared fish were compared to that of D. dentex juveniles, collected from the natural environment. All the wild juveniles were normal in respect to their osteological appearance, while the reared specimens presented skeletal deformities resulting from different rearing methods applied. Saddleback syndrome (4.0-4.4%) and severe external deformities of the caudal fin (14.3-15.0%) characterised exclusively the semi-extensive populations, while the extensive populations presented severe abnormalities of the pre-ural centra (25.0-25.6%) with significantly higher frequency than the S reared fish (5.8%). Saddleback syndrome was expressed as a lack of one to all the hard spines of the dorsal fin, accompanied by shape, number and position abnormalities of the related pterygiophores. Caudal fin deformities were mainly characterised by the lack of the upper lepidotrichia or/and dermatotrichia, accompanied by severe deformities of the supporting elements of the upper lobe. The saddleback syndrome and severe caudal fin deformities were anatomically and ontogenetically related to each other, originating at the early larval stage as a result of abnormalities of the primordial marginal finfold and of the posterior tip of the notochord. In respect to the dominant phenotypes, the meristic characters of the normal reared fish were not differentiated from those of the wild, but they presented higher variability. The results are discussed in view of the aetiology, applications and research targets for the erasure of skeletal malformations. © 2001 Elsevier Science B.V. All rights reserved. |
Koumoundouros, G; Divanach, P; Kentouri, M Osteological development of Dentex dentex (Osteichthyes: Sparidae): dorsal, anal, paired fins and squamation Journal Article Marine Biology, 138 (2), pp. 399–406, 2001, ISSN: 0025-3162, 1432-1793. @article{koumoundouros_osteological_2001-1, title = {Osteological development of Dentex dentex (Osteichthyes: Sparidae): dorsal, anal, paired fins and squamation}, author = {G Koumoundouros and P Divanach and M Kentouri}, url = {http://link.springer.com/10.1007/s002270000460}, doi = {10.1007/s002270000460}, issn = {0025-3162, 1432-1793}, year = {2001}, date = {2001-02-01}, urldate = {2020-08-18}, journal = {Marine Biology}, volume = {138}, number = {2}, pages = {399--406}, abstract = {The osteological development of dorsal, anal and paired fins and the squamation in common dentex, Dentex dentex (Linnaeus, 1758), was studied under extensive larval rearing conditions. The ontogeny of the fins was determined in three stages: (1) the yolk-sac and the early larval stage (up to 6.7 mm in vivo total length, TL), in which the majority of the elements of the pectoral fins and the predorsals, dorsal and anal proximal pterygiophores formed; (2) the late larval stage (up to 11.6 mm TL), in which the majority of the pectoral lepidotrichia, the lower extrascapulae, all the elements of the pelvic fins, as well as the lepidotrichia, spines and the distal radials of the rest fins developed; and (3) the metamorphosis stage (up to 24.0 mm TL), when the upper extrascapulae formed and the pectoral lepidotrichia attained their full count. The ontogeny of squamation was carried out during the metamorphosis stage; it started at 13.4 mm TL with laterally symmetric points on the middle of each side of the body, followed by one ventral centre (15.6 mm TL) which was located anterior to the pelvic fins, and two bilateral pairs of centres which appeared on the head (17.8 and 21.2 mm TL). The results are discussed from a functional viewpoint and in comparison with the fin and scale ontogeny of other teleosts.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The osteological development of dorsal, anal and paired fins and the squamation in common dentex, Dentex dentex (Linnaeus, 1758), was studied under extensive larval rearing conditions. The ontogeny of the fins was determined in three stages: (1) the yolk-sac and the early larval stage (up to 6.7 mm in vivo total length, TL), in which the majority of the elements of the pectoral fins and the predorsals, dorsal and anal proximal pterygiophores formed; (2) the late larval stage (up to 11.6 mm TL), in which the majority of the pectoral lepidotrichia, the lower extrascapulae, all the elements of the pelvic fins, as well as the lepidotrichia, spines and the distal radials of the rest fins developed; and (3) the metamorphosis stage (up to 24.0 mm TL), when the upper extrascapulae formed and the pectoral lepidotrichia attained their full count. The ontogeny of squamation was carried out during the metamorphosis stage; it started at 13.4 mm TL with laterally symmetric points on the middle of each side of the body, followed by one ventral centre (15.6 mm TL) which was located anterior to the pelvic fins, and two bilateral pairs of centres which appeared on the head (17.8 and 21.2 mm TL). The results are discussed from a functional viewpoint and in comparison with the fin and scale ontogeny of other teleosts. |
Papandroulakis, N; Divanach, P; Anastasiadis, P; Kentouri, M The pseudo-green water technique for intensive rearing of sea bream (Sparus aurata) larvae Journal Article Aquaculture International, 9 , pp. 205 – 216, 2001. @article{papandroulakis_pseudo-green_2001, title = {The pseudo-green water technique for intensive rearing of sea bream (Sparus aurata) larvae}, author = {N Papandroulakis and P Divanach and P Anastasiadis and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035741183&partnerID=40&md5=25026d07e6e77837a60e0648627158db}, year = {2001}, date = {2001-01-01}, journal = {Aquaculture International}, volume = {9}, pages = {205 -- 216}, abstract = {The "pseudo green water" technology for sea bream (Sparus aurata) larval rearing was evaluated by analyzing results of a 2-year study, performed in a pilot scale system. The technology is characterized by the daily addition of phytoplankton in the rearing tanks during the first month of rearing. Effects of egg origin, spawning season and initial larval density on the larval rearing were investigated. Fish reared in "pseudo green water" systems for 60 days, presented high biological performance in terms of survival (56 ± 16%), mean weight (62 ± 12 mg), total length (20 ± 1 mm), conformity with wild standard (88 ± 9%), and functional swim bladder (93 ± 4%). The results present homogenity between the categories (origin of eggs, time of spawning, initial egg density) studied, proving the stability of the technology.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The "pseudo green water" technology for sea bream (Sparus aurata) larval rearing was evaluated by analyzing results of a 2-year study, performed in a pilot scale system. The technology is characterized by the daily addition of phytoplankton in the rearing tanks during the first month of rearing. Effects of egg origin, spawning season and initial larval density on the larval rearing were investigated. Fish reared in "pseudo green water" systems for 60 days, presented high biological performance in terms of survival (56 ± 16%), mean weight (62 ± 12 mg), total length (20 ± 1 mm), conformity with wild standard (88 ± 9%), and functional swim bladder (93 ± 4%). The results present homogenity between the categories (origin of eggs, time of spawning, initial egg density) studied, proving the stability of the technology. |
Rigos, G; Pavlidis, M; Divanach, P Host susceptibility to Cryptocaryon sp. infection of Mediterranean marine broodfish held under intensive culture conditions: A case report Journal Article Bulletin of the European Association of Fish Pathologists, 21 , pp. 33 – 36, 2001. @article{rigos_host_2001, title = {Host susceptibility to Cryptocaryon sp. infection of Mediterranean marine broodfish held under intensive culture conditions: A case report}, author = {G Rigos and M Pavlidis and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0002831958&partnerID=40&md5=265b03fc4289569f541da14d6b89a6c8}, year = {2001}, date = {2001-01-01}, journal = {Bulletin of the European Association of Fish Pathologists}, volume = {21}, pages = {33 -- 36}, abstract = {The detrimental effect of Cryptocaryon irritans exhibiting a host specificity on several marine fish broodstock is described in this study. The parasite caused considerable mortalities on gilthead seabream, Sparus aurata, common dentex, Dentex dentex, and greater amberjack, Seriola dumerili, while red porgy, Pagrus pagrus, white sea bream, Diplodus sargus, sea bass, Dicentrarchus labrax, and sharpsnout sea bream, Diplodus puntazzo, remained unaffected. Chemical treatments were not found to be effective against the intense parasitosis and only extended, rapid and repeated hyposalinity measures seemed to minimise fish losses.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The detrimental effect of Cryptocaryon irritans exhibiting a host specificity on several marine fish broodstock is described in this study. The parasite caused considerable mortalities on gilthead seabream, Sparus aurata, common dentex, Dentex dentex, and greater amberjack, Seriola dumerili, while red porgy, Pagrus pagrus, white sea bream, Diplodus sargus, sea bass, Dicentrarchus labrax, and sharpsnout sea bream, Diplodus puntazzo, remained unaffected. Chemical treatments were not found to be effective against the intense parasitosis and only extended, rapid and repeated hyposalinity measures seemed to minimise fish losses. |
Carrillo, J; Koumoundouros, G; Divanach, P; Martinez, J Morphological malformations of the lateral line in reared gilthead sea bream (Sparus aurata L. 1758) Journal Article Aquaculture, 192 (2-4), pp. 281–290, 2001, ISSN: 00448486. @article{carrillo_morphological_2001, title = {Morphological malformations of the lateral line in reared gilthead sea bream (Sparus aurata L. 1758)}, author = {J Carrillo and G Koumoundouros and P Divanach and J Martinez}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848600004543}, doi = {10.1016/S0044-8486(00)00454-3}, issn = {00448486}, year = {2001}, date = {2001-01-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {192}, number = {2-4}, pages = {281--290}, abstract = {A general description of the morphological modifications of the lateral line in gilthead sea bream, reared under hatchery conditions, is provided. Farm-reared fish were examined and compared to wild fish. We used the presence of a pore on the specialized scales of the lateral line to determine the density and distribution of associated sensory neuromasts. The malformations of the lateral line were: zigzag with missing sector, wavy with missing sector, several scattered missing sectors, and several consecutive missing sectors. These abnormalities occurred in 71.0-86.2% of the reared fish, depending on the hatchery. They did not occur in wild fish. The possible behaviour consequences for gilthead sea bream of deficient sensory reception are considered. In addition, the suitability of these abnormalities for use as individual markers in different types of studies is proposed. © 2001 Elsevier Science B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A general description of the morphological modifications of the lateral line in gilthead sea bream, reared under hatchery conditions, is provided. Farm-reared fish were examined and compared to wild fish. We used the presence of a pore on the specialized scales of the lateral line to determine the density and distribution of associated sensory neuromasts. The malformations of the lateral line were: zigzag with missing sector, wavy with missing sector, several scattered missing sectors, and several consecutive missing sectors. These abnormalities occurred in 71.0-86.2% of the reared fish, depending on the hatchery. They did not occur in wild fish. The possible behaviour consequences for gilthead sea bream of deficient sensory reception are considered. In addition, the suitability of these abnormalities for use as individual markers in different types of studies is proposed. © 2001 Elsevier Science B.V. |
2000 |
Maurizi, A; Diaz, J P; Divanach, P; Papandroulakis, N; Connes, R The effect of glycerol dissolved in the rearing water on the transition to exotrophy in gilthead sea bream Sparus aurata larvae Journal Article Aquaculture, 189 , pp. 119 – 131, 2000. @article{maurizi_effect_2000, title = {The effect of glycerol dissolved in the rearing water on the transition to exotrophy in gilthead sea bream Sparus aurata larvae}, author = {A Maurizi and J P Diaz and P Divanach and N Papandroulakis and R Connes}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034715335&partnerID=40&md5=2ad2493b26d28332d0f11b019e7a552a}, year = {2000}, date = {2000-01-01}, journal = {Aquaculture}, volume = {189}, pages = {119 -- 131}, abstract = {The effects of glycerol added to seawater at different concentrations were tested in monocultures of Chlorella minutissima and Brachionus plicatilis in mixed cultures of these two species and on starved or fed Sparus aurata larvae. Glycerol enhanced the growth of Chlorella and seemed to indirectly benefit the rotifers that feed on it. Its beneficial effect on gilthead sea bream prelarvae and larvae resulted in glycogen accumulation in the liver and absence of signs of cholestasis-like pathology. This effect varied according to the glycerol concentration in the water, to its addition timing and to light and feeding conditions. The gluconeogenic origin of the hepatic glycogen reserves is discussed, together with the mediating role of the food chain in glycerol larval assimilation. (C) 2000 Elsevier Science B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The effects of glycerol added to seawater at different concentrations were tested in monocultures of Chlorella minutissima and Brachionus plicatilis in mixed cultures of these two species and on starved or fed Sparus aurata larvae. Glycerol enhanced the growth of Chlorella and seemed to indirectly benefit the rotifers that feed on it. Its beneficial effect on gilthead sea bream prelarvae and larvae resulted in glycogen accumulation in the liver and absence of signs of cholestasis-like pathology. This effect varied according to the glycerol concentration in the water, to its addition timing and to light and feeding conditions. The gluconeogenic origin of the hepatic glycogen reserves is discussed, together with the mediating role of the food chain in glycerol larval assimilation. (C) 2000 Elsevier Science B.V. |
Koumoundouros, G; Divanach, P; Savaki, A; Kentouri, M Effects of three preservation methods on the evolution of swimbladder radiographic appearance in sea bass and sea bream juveniles Journal Article Aquaculture, 182 , pp. 17 – 25, 2000. @article{koumoundouros_effects_2000, title = {Effects of three preservation methods on the evolution of swimbladder radiographic appearance in sea bass and sea bream juveniles}, author = {G Koumoundouros and P Divanach and A Savaki and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034142216&partnerID=40&md5=676bd624fd0f9054b964b990f7787846}, doi = {https://doi.org/10.1016/S0044-8486(99)00249-5}, year = {2000}, date = {2000-01-01}, journal = {Aquaculture}, volume = {182}, pages = {17 -- 25}, abstract = {Sea bass and sea bream juveniles with functional swimbladder were exposed to three methods of preservation: (a) in 10% phosphate buffered formalin, (b) at 4°C, and (c) at -25°C. They were then X-rayed daily over 10 days to evaluate the impact of these methods on the evolution of the radiographic image. All methods significantly decreased the apparent length and depth of the swimbladder, but with different intensities. Freezing had the least effect (9% and 12% reduction in bream and bass, respectively) and only during the first day of preservation. Four days of formalin fixation led to the total disappearance of this image. Preservation at 4°C led to the autolysis and putrefaction of the internal organs which was responsible for the poor images after three days. The results were discussed in terms of their use for the assessment of Juvenile quality by radiography.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Sea bass and sea bream juveniles with functional swimbladder were exposed to three methods of preservation: (a) in 10% phosphate buffered formalin, (b) at 4°C, and (c) at -25°C. They were then X-rayed daily over 10 days to evaluate the impact of these methods on the evolution of the radiographic image. All methods significantly decreased the apparent length and depth of the swimbladder, but with different intensities. Freezing had the least effect (9% and 12% reduction in bream and bass, respectively) and only during the first day of preservation. Four days of formalin fixation led to the total disappearance of this image. Preservation at 4°C led to the autolysis and putrefaction of the internal organs which was responsible for the poor images after three days. The results were discussed in terms of their use for the assessment of Juvenile quality by radiography. |
Moschou, E A; Lasarte, Azpiroz U; Fouskaki, M; Chaniotakis, N A; Papandroulakis, N; Divanach, P Direct electrochemical flow analysis system for simultaneous monitoring of total ammonia and nitrite in seawater Journal Article Aquacultural Engineering, 22 , pp. 255 – 268, 2000. @article{moschou_direct_2000, title = {Direct electrochemical flow analysis system for simultaneous monitoring of total ammonia and nitrite in seawater}, author = {E A Moschou and U Azpiroz Lasarte and M Fouskaki and N A Chaniotakis and N Papandroulakis and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034128510&partnerID=40&md5=b82b0b1ee44766fb1f6d6e77b70ec3a9}, year = {2000}, date = {2000-01-01}, journal = {Aquacultural Engineering}, volume = {22}, pages = {255 -- 268}, abstract = {The design and the performance of a new portable flow analysis (FA) system for the continuous, fast and accurate measurement of total ammonia and nitrite content in non-filtered seawater samples is demonstrated. The complete system has been optimized to operate within the ammonia and nitrite concentration range of 0.05-10 ppm. The system offers good reproducibility (textless 5%) and stability (textless 0.02 ppm/h) at constant temperature, while the analysis time is in the order of 1.5-4 min depending on the sample analyzed. The analysis results of seawater samples obtained with the FA system were compared to that obtained with the standard colorimetric method and establish the suitability of the analyzer for the precise and continuous measurements of untreated samples for both in field and laboratory applications. In addition, its small size and weight offer the advantage of portability, while its datalogging capabilities also allow for independent ammonia and nitrite monitoring. (C) 2000 Elsevier Science B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The design and the performance of a new portable flow analysis (FA) system for the continuous, fast and accurate measurement of total ammonia and nitrite content in non-filtered seawater samples is demonstrated. The complete system has been optimized to operate within the ammonia and nitrite concentration range of 0.05-10 ppm. The system offers good reproducibility (textless 5%) and stability (textless 0.02 ppm/h) at constant temperature, while the analysis time is in the order of 1.5-4 min depending on the sample analyzed. The analysis results of seawater samples obtained with the FA system were compared to that obtained with the standard colorimetric method and establish the suitability of the analyzer for the precise and continuous measurements of untreated samples for both in field and laboratory applications. In addition, its small size and weight offer the advantage of portability, while its datalogging capabilities also allow for independent ammonia and nitrite monitoring. (C) 2000 Elsevier Science B.V. |
Pavlidis, M; Koumoundouros, G; Sterioti, A; Somarakis, S; Divanach, P; Kentouri, M Evidence of temperature-dependent sex determination in the european sea bass (Dicentrarchus labrax L.) Journal Article Journal of Experimental Zoology, 287 , pp. 225 – 232, 2000. @article{pavlidis_evidence_2000, title = {Evidence of temperature-dependent sex determination in the european sea bass (Dicentrarchus labrax L.)}, author = {M Pavlidis and G Koumoundouros and A Sterioti and S Somarakis and P Divanach and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034255757&partnerID=40&md5=2d194d1edd776c20c4fe6c96e412b855}, year = {2000}, date = {2000-01-01}, journal = {Journal of Experimental Zoology}, volume = {287}, pages = {225 -- 232}, abstract = {To test the hypothesis that sex determination in the European sea bass (Dicentrarchus labrax L.) can be affected by the incubating temperature during the very early developmental stages, eggs from the same batch of spontaneously spawned broodstock were divided at the stage of half-epiboly into three groups according to rearing temperature: G13 = 13°C}, keywords = {}, pubstate = {published}, tppubtype = {article} } To test the hypothesis that sex determination in the European sea bass (Dicentrarchus labrax L.) can be affected by the incubating temperature during the very early developmental stages, eggs from the same batch of spontaneously spawned broodstock were divided at the stage of half-epiboly into three groups according to rearing temperature: G13 = 13°C |
Pavlidis, M; Greenwood, L; Mourot, B; Kokkari, C; Menn, Le F; Divanach, P; Scott, A P General and Comparative Endocrinology, 118 , pp. 14 – 25, 2000. @article{pavlidis_seasonal_2000, title = {Seasonal variations and maturity stages in relation to differences in serum levels of gonadal steroids, vitellogenin, and thyroid hormones in the common dentex (Dentex dentex)}, author = {M Pavlidis and L Greenwood and B Mourot and C Kokkari and F Le Menn and P Divanach and A P Scott}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034006188&partnerID=40&md5=d5286b0db60ae52af7841e4963700b70}, year = {2000}, date = {2000-01-01}, journal = {General and Comparative Endocrinology}, volume = {118}, pages = {14 -- 25}, abstract = {Seasonal variations in serum concentrations of 17β-estradiol (E2), vitellogenin (Vg), testosterone (T), 11 ketotestosterone (11-KT), and thyroid hormones (T4, L-thyroxine; and T3, 3,5,3’-triiodo-L-thyronine) were investigated during the first, second, and third reproductive cycles in intensively reared populations of common dentex, Dentex dentex, and correlated with gonadal development and spawning. In females, there were baseline E2 values (<0.10 ng/ml) and negligible Vg concentrations during the postspawning and pregametogenesis period (June to December), and these increased thereafter to peak during the spawning period. Maximum T3 and T4 serum concentrations were found around spawning. There was a positive correlation during vitellogenesis and final maturation between Vg and T3 (r2 = 0.366). In addition, Vg and T3 concentrations were statistically higher in the stages of vitellogenesis and final maturation than at the other stages (P < 0.001). Minimum T3 and T4 concentrations (October) coincided with the decrease in water temperature and the associated decrease in the daily feeding rate and the specific growth rate. In males, as in females, seasonal changes in serum levels of T and 11-KT were well correlated with gonadal development. The presence of males in the stage of completed spermiogenesis in December coincided with the surge in both androgens and this increase lasted until the end of the spawning period. There were no significant differences in serum T3 and T4 levels among the maturity stages. The observed seasonal changes in serum gonadal steroids and Vg reflected the pattern of oocyte development and the spawning behavior of common dentex and were typical of the patterns described in most multiple spawners studied to date. Thyroid hormones may enhance early ovarian development and stimulate vitellogenesis in female dentex. (C) 2000 Academic Press.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Seasonal variations in serum concentrations of 17β-estradiol (E2), vitellogenin (Vg), testosterone (T), 11 ketotestosterone (11-KT), and thyroid hormones (T4, L-thyroxine; and T3, 3,5,3’-triiodo-L-thyronine) were investigated during the first, second, and third reproductive cycles in intensively reared populations of common dentex, Dentex dentex, and correlated with gonadal development and spawning. In females, there were baseline E2 values (<0.10 ng/ml) and negligible Vg concentrations during the postspawning and pregametogenesis period (June to December), and these increased thereafter to peak during the spawning period. Maximum T3 and T4 serum concentrations were found around spawning. There was a positive correlation during vitellogenesis and final maturation between Vg and T3 (r2 = 0.366). In addition, Vg and T3 concentrations were statistically higher in the stages of vitellogenesis and final maturation than at the other stages (P < 0.001). Minimum T3 and T4 concentrations (October) coincided with the decrease in water temperature and the associated decrease in the daily feeding rate and the specific growth rate. In males, as in females, seasonal changes in serum levels of T and 11-KT were well correlated with gonadal development. The presence of males in the stage of completed spermiogenesis in December coincided with the surge in both androgens and this increase lasted until the end of the spawning period. There were no significant differences in serum T3 and T4 levels among the maturity stages. The observed seasonal changes in serum gonadal steroids and Vg reflected the pattern of oocyte development and the spawning behavior of common dentex and were typical of the patterns described in most multiple spawners studied to date. Thyroid hormones may enhance early ovarian development and stimulate vitellogenesis in female dentex. (C) 2000 Academic Press. |
Papandroulakis, N; Markakis, G; Divanach, P; Kentouri, M Feeding requirements of sea bream (Sparus aurata) larvae under intensive rearing conditions: Development of a fuzzy logic controller for feeding Journal Article Aquacultural Engineering, 21 , pp. 285 – 299, 2000. @article{papandroulakis_feeding_2000, title = {Feeding requirements of sea bream (Sparus aurata) larvae under intensive rearing conditions: Development of a fuzzy logic controller for feeding}, author = {N Papandroulakis and G Markakis and P Divanach and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034008999&partnerID=40&md5=08b95efaa79a087e5c80a9df9872afc1}, year = {2000}, date = {2000-01-01}, journal = {Aquacultural Engineering}, volume = {21}, pages = {285 -- 299}, abstract = {Applied methods for larval rearing suffer from the difficulty of accurately predicting larvae quantitative feeding requirements for optimum growth. Even when known, requirements change according to the behavior of each population reared. Computer based feeding systems require tools for adapting the delivered amounts of food to the needs of the reared population. In this work the daily feeding requirements of sea bream (Sparus aurata) larvae were estimated, and based on these estimations a fuzzy logic controller (FLC) for feeding was designed and developed. Sea bream larvae were reared, in triplicate, under intensive conditions in a pilot scale rearing system for 20 days with the pseudo-green water method. Larvae reached 6.7 ± 0.6 mm (mean value and S.D.) at the end of the experimental period while the mean survival of the populations was 48.0 ± 12.6%. Consumption was 0.017 ± 0.009 mg of carbon per individual at first feeding and increased to 0.198 ± 0.032 mg at day 20 post-hatching. The findings were used for the development of a FLC for feeding that adapts the amount of food delivered to the larvae according to changes in the reared population. The FLC utilizes five linguistic variables describing the state of the population and a rule base composed of 316 rules. The developed FLC allows the use of computer based feeding systems during rearing of early larval stages. (C) 2000 Elsevier Science B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Applied methods for larval rearing suffer from the difficulty of accurately predicting larvae quantitative feeding requirements for optimum growth. Even when known, requirements change according to the behavior of each population reared. Computer based feeding systems require tools for adapting the delivered amounts of food to the needs of the reared population. In this work the daily feeding requirements of sea bream (Sparus aurata) larvae were estimated, and based on these estimations a fuzzy logic controller (FLC) for feeding was designed and developed. Sea bream larvae were reared, in triplicate, under intensive conditions in a pilot scale rearing system for 20 days with the pseudo-green water method. Larvae reached 6.7 ± 0.6 mm (mean value and S.D.) at the end of the experimental period while the mean survival of the populations was 48.0 ± 12.6%. Consumption was 0.017 ± 0.009 mg of carbon per individual at first feeding and increased to 0.198 ± 0.032 mg at day 20 post-hatching. The findings were used for the development of a FLC for feeding that adapts the amount of food delivered to the larvae according to changes in the reared population. The FLC utilizes five linguistic variables describing the state of the population and a rule base composed of 316 rules. The developed FLC allows the use of computer based feeding systems during rearing of early larval stages. (C) 2000 Elsevier Science B.V. |
Koumoundouros, G; Divanach, P; Kentouri, M Development of the skull in Dentex dentex (Osteichthyes: Sparidae) Journal Article Marine Biology, 136 (1), pp. 175–184, 2000, ISSN: 0025-3162, 1432-1793. @article{koumoundouros_development_2000, title = {Development of the skull in Dentex dentex (Osteichthyes: Sparidae)}, author = {G Koumoundouros and P Divanach and M Kentouri}, url = {http://link.springer.com/10.1007/s002270050020}, doi = {10.1007/s002270050020}, issn = {0025-3162, 1432-1793}, year = {2000}, date = {2000-01-01}, urldate = {2020-08-17}, journal = {Marine Biology}, volume = {136}, number = {1}, pages = {175--184}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
1999 |
Koumoundouros, G; Divanach, P; Kentouri, M Ontogeny and allometric plasticity of Dentex dentex (Osteichthyes: Sparidae) in rearing conditions Journal Article Marine Biology, 135 (3), pp. 561–572, 1999, ISSN: 0025-3162, 1432-1793. @article{koumoundouros_ontogeny_1999, title = {Ontogeny and allometric plasticity of Dentex dentex (Osteichthyes: Sparidae) in rearing conditions}, author = {G Koumoundouros and P Divanach and M Kentouri}, url = {http://link.springer.com/10.1007/s002270050657}, doi = {10.1007/s002270050657}, issn = {0025-3162, 1432-1793}, year = {1999}, date = {1999-12-01}, urldate = {2020-08-18}, journal = {Marine Biology}, volume = {135}, number = {3}, pages = {561--572}, abstract = {The ontogeny of Dentex dentex (Linnaeus, 1758) was studied comparatively under extensive and semi-extensive rearing conditions. The exotrophic phase was divided into three distinct developmental stages (a) larval, from feeding onset up to 10 mm total length (TL); (b) metamorphosis, from 10 mm TL up to 24 mm TL; and (c) further juveniles. The majority of all ontogenic changes was expressed during the larval stage and integrated during the metamorphosis stage. The juvenile stage was characterized by developmental stability. The normal development of D. dentex was seriously affected by the rearing conditions applied during the early exotrophic phase. The two differently reared populations followed similar developmental patterns of general morphology, pigmentation and behaviour, but they presented high developmental plasticity where allometric growth was concerned. Rearing conditions influenced both the boundaries of the sequential stages (in 50% of the morphometric ratios) and the allometry coefficient of each morphometric character in the various developmental stages (in 80% of the characters). The results are discussed with respect to the ontogeny of the functional morphology under both ecological and aquaculture considerations.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The ontogeny of Dentex dentex (Linnaeus, 1758) was studied comparatively under extensive and semi-extensive rearing conditions. The exotrophic phase was divided into three distinct developmental stages (a) larval, from feeding onset up to 10 mm total length (TL); (b) metamorphosis, from 10 mm TL up to 24 mm TL; and (c) further juveniles. The majority of all ontogenic changes was expressed during the larval stage and integrated during the metamorphosis stage. The juvenile stage was characterized by developmental stability. The normal development of D. dentex was seriously affected by the rearing conditions applied during the early exotrophic phase. The two differently reared populations followed similar developmental patterns of general morphology, pigmentation and behaviour, but they presented high developmental plasticity where allometric growth was concerned. Rearing conditions influenced both the boundaries of the sequential stages (in 50% of the morphometric ratios) and the allometry coefficient of each morphometric character in the various developmental stages (in 80% of the characters). The results are discussed with respect to the ontogeny of the functional morphology under both ecological and aquaculture considerations. |
Koumoundouros, G; Divanach, P; Kentouri, M Osteological development of the vertebral column and of the caudal complex in Dentex dentex Journal Article Journal of Fish Biology, 54 (2), pp. 424–436, 1999, ISSN: 0022-1112, 1095-8649. @article{koumoundouros_osteological_1999, title = {Osteological development of the vertebral column and of the caudal complex in Dentex dentex}, author = {G Koumoundouros and P Divanach and M Kentouri}, url = {http://doi.wiley.com/10.1111/j.1095-8649.1999.tb00841.x}, doi = {10.1111/j.1095-8649.1999.tb00841.x}, issn = {0022-1112, 1095-8649}, year = {1999}, date = {1999-02-01}, urldate = {2020-08-18}, journal = {Journal of Fish Biology}, volume = {54}, number = {2}, pages = {424--436}, abstract = {Osteological development of the vertebral column and caudal complex in common dentex was described under extensive larval rearing conditions. Generally, the cartilaginous bones developed prior to the membranous bones. The development of the axial skeleton began with the formation of the hypural 1, the neural arches 2 and 3, as well as the haemal arches 1-8 at 4.8, 4.9 and 5.0 mm total length (L(T), measured in vivo), respectively. By 7.5 mm L(T), all the cartilaginous elements were formed, except for the ventral ribs, which formed between the range of 8.4-18.0 mm L(T) The caudal lepidotrichia were the first membranous bones to appear (5.3 mm L(T)) and attain their full meristic count (7.4 mm L(T)), followed by the vertebral centra, which formed between 6.6 and 9.7 mm L(T). By 25.0 mm L(T), all the elements were fully ossified except for the ventral ribs. The developmental direction and order of all the elements were studied with respect to their formation and ossification. The results were discussed in the contexts of ichthyoplankton, ecology and aquaculture. Compared with other Sparidae species, common dentex followed a pattern of relatively rapid rate of osteological development.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Osteological development of the vertebral column and caudal complex in common dentex was described under extensive larval rearing conditions. Generally, the cartilaginous bones developed prior to the membranous bones. The development of the axial skeleton began with the formation of the hypural 1, the neural arches 2 and 3, as well as the haemal arches 1-8 at 4.8, 4.9 and 5.0 mm total length (L(T), measured in vivo), respectively. By 7.5 mm L(T), all the cartilaginous elements were formed, except for the ventral ribs, which formed between the range of 8.4-18.0 mm L(T) The caudal lepidotrichia were the first membranous bones to appear (5.3 mm L(T)) and attain their full meristic count (7.4 mm L(T)), followed by the vertebral centra, which formed between 6.6 and 9.7 mm L(T). By 25.0 mm L(T), all the elements were fully ossified except for the ventral ribs. The developmental direction and order of all the elements were studied with respect to their formation and ossification. The results were discussed in the contexts of ichthyoplankton, ecology and aquaculture. Compared with other Sparidae species, common dentex followed a pattern of relatively rapid rate of osteological development. |
Carrillo, J; Martinez, J; Divanach, P; Kentouri, M Unilateral eye abnormalities in reared Mediterranean gilthead sea bream Journal Article Veterinary Record, 145 , pp. 494 – 497, 1999. @article{carrillo_unilateral_1999, title = {Unilateral eye abnormalities in reared Mediterranean gilthead sea bream}, author = {J Carrillo and J Martinez and P Divanach and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0033598482&partnerID=40&md5=3137530bf8b7e7dcb44d7cc2eae77911}, doi = {http://dx.doi.org/10.1136/vr.145.17.494}, year = {1999}, date = {1999-01-01}, journal = {Veterinary Record}, volume = {145}, pages = {494 -- 497}, abstract = {Eye abnormalities in reared gilthead sea bream (Sparus aurato) were investigated clinically and by histological techniques. A significant number of fish had a cataract in one eye and ocular inflammation including gross exophthalmos. In a small number of fish gas bubbles were observed in the anterior chamber of the eye. Histological examination provided no evidence of an infectious process and a possible behavioural aetiology is discussed.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Eye abnormalities in reared gilthead sea bream (Sparus aurato) were investigated clinically and by histological techniques. A significant number of fish had a cataract in one eye and ocular inflammation including gross exophthalmos. In a small number of fish gas bubbles were observed in the anterior chamber of the eye. Histological examination provided no evidence of an infectious process and a possible behavioural aetiology is discussed. |
Pavlidis, M; Paspatis, M; Koistinen, M; Paavola, T; Divanach, P; Kentouri, M Diel rhythms of serum metabolites and thyroid hormones in red porgy held in different photoperiod regimes Journal Article Aquaculture International, 7 , pp. 29 – 44, 1999. @article{pavlidis_diel_1999, title = {Diel rhythms of serum metabolites and thyroid hormones in red porgy held in different photoperiod regimes}, author = {M Pavlidis and M Paspatis and M Koistinen and T Paavola and P Divanach and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0032712520&partnerID=40&md5=1435b4ae75810998a0eeb86912538d21}, year = {1999}, date = {1999-01-01}, journal = {Aquaculture International}, volume = {7}, pages = {29 -- 44}, abstract = {Diel rhythms in serum glucose, lactate, cholesterol, triglycerides and thyroid hormones were studied in red porgy, Pagrus pagrus, held under different photoperiod regimes (constant darkness - DD, 8L:16D,12L:12D), at a constant temperature (17.1-18.7 °C) and fed with commercial pellets, by means of a self-feeder. A clear diurnal rhythm in feeding activity, regardless of the photoperiod regime was demonstrated. All serum components showed significant diel rhythms, although they were not necessarily consistent or circadian in periodicity. As well as this, differences in the average values among the varying treatments were observed. Fish held under the 12L:12D protocol displayed significantly higher average T4, T3 and lactate levels during the day rather than at night. Maximum glucose values occurred 8-12 h after dawn and maximum lactate concentrations at 06:00 or 14:00 h. Diel variations in cholesterol were evident only in the DD group with peak values inversely correlated with the rhythm of food intake. Triglycerides displayed a similar pattern of changes. Significant diel fluctuations in T4 serum levels were observed only in fish exposed to the 12L:12D protocol, with peak values at dawn. A clear diurnal peak (10:00h) in T3 concentrations was observed in fish subjected to the 12L:12D regime, while fish exposed to the 8L:16D protocol showed two peaks: one in the photophase (10:00 h) and another in the scotophase (02:00 h). The light-dark alternation and the general activity rhythm of fish seem to be the main synchronizers of the diel rhythms observed in this study.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Diel rhythms in serum glucose, lactate, cholesterol, triglycerides and thyroid hormones were studied in red porgy, Pagrus pagrus, held under different photoperiod regimes (constant darkness - DD, 8L:16D,12L:12D), at a constant temperature (17.1-18.7 °C) and fed with commercial pellets, by means of a self-feeder. A clear diurnal rhythm in feeding activity, regardless of the photoperiod regime was demonstrated. All serum components showed significant diel rhythms, although they were not necessarily consistent or circadian in periodicity. As well as this, differences in the average values among the varying treatments were observed. Fish held under the 12L:12D protocol displayed significantly higher average T4, T3 and lactate levels during the day rather than at night. Maximum glucose values occurred 8-12 h after dawn and maximum lactate concentrations at 06:00 or 14:00 h. Diel variations in cholesterol were evident only in the DD group with peak values inversely correlated with the rhythm of food intake. Triglycerides displayed a similar pattern of changes. Significant diel fluctuations in T4 serum levels were observed only in fish exposed to the 12L:12D protocol, with peak values at dawn. A clear diurnal peak (10:00h) in T3 concentrations was observed in fish subjected to the 12L:12D regime, while fish exposed to the 8L:16D protocol showed two peaks: one in the photophase (10:00 h) and another in the scotophase (02:00 h). The light-dark alternation and the general activity rhythm of fish seem to be the main synchronizers of the diel rhythms observed in this study. |
Kotzabasis, K; Hatziathanasiou, A; Bengoa-Ruigomez, M V; Kentouri, M; Divanach, P Progress in Industrial Microbiology, 35 , pp. 357–362, Elsevier, 1999, ISBN: 978-0-444-50387-9. @incollection{kotzabasis_methanol_1999, title = {Methanol as alternative carbon source for quicker efficient production of the microalgae Chlorella minutissima: Role of the concentration and frequence of administration}, author = {K Kotzabasis and A Hatziathanasiou and M V Bengoa-Ruigomez and M Kentouri and P Divanach}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0079635299801283}, doi = {10.1016/S0079-6352(99)80128-3}, isbn = {978-0-444-50387-9}, year = {1999}, date = {1999-01-01}, urldate = {2020-08-18}, booktitle = {Progress in Industrial Microbiology}, volume = {35}, pages = {357--362}, publisher = {Elsevier}, abstract = {Autotrophic cultures of the marine microalgae Chlorella minutissima were performed at 13 000 lux continuous illumination in 11 chambers fertilised with 0.25 g l - 1 F2 medium and different doses of methanol. This was administered in two ways during two parallel experimental series of 10 days: 0.05, 0.1, 0.5, 1.0 and 5.0% methanol (v/v) in one unique dose at the beginning of the culture and 110 of these (i.e. 0.005, 0.01, 0.05, 0.1 and 0.5% methanol (v/v)) in daily doses for the 10-day culture period. Low concentrations of methanol induced a faster increase of cell density and dry weight than control, while high concentrations induced symptoms of toxicity. The higher cell densities and quicker growth were observed in the experiments with daily administration of 0.005 and 0.1% (v/v) methanol, while those with one dose presented an initial boosted growth but a final cell density lower than control. The role of methanol as alternative carbon source for microalgae, as well as its possible impact on the quality of biomass production and on the environment, are discussed. © 1999 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {incollection} } Autotrophic cultures of the marine microalgae Chlorella minutissima were performed at 13 000 lux continuous illumination in 11 chambers fertilised with 0.25 g l - 1 F2 medium and different doses of methanol. This was administered in two ways during two parallel experimental series of 10 days: 0.05, 0.1, 0.5, 1.0 and 5.0% methanol (v/v) in one unique dose at the beginning of the culture and 110 of these (i.e. 0.005, 0.01, 0.05, 0.1 and 0.5% methanol (v/v)) in daily doses for the 10-day culture period. Low concentrations of methanol induced a faster increase of cell density and dry weight than control, while high concentrations induced symptoms of toxicity. The higher cell densities and quicker growth were observed in the experiments with daily administration of 0.005 and 0.1% (v/v) methanol, while those with one dose presented an initial boosted growth but a final cell density lower than control. The role of methanol as alternative carbon source for microalgae, as well as its possible impact on the quality of biomass production and on the environment, are discussed. © 1999 Elsevier B.V. All rights reserved. |
1998 |
Rueda, F M; Martinez, F J; Zamora, S; Kentouri, M; Divanach, P Effect of fasting and refeeding on growth and body composition of red porgy, Pagrus pagrus L.* Journal Article Aquaculture Research, 29 (6), pp. 447–452, 1998, ISSN: 1355-557X, 1365-2109. @article{rueda_effect_1998, title = {Effect of fasting and refeeding on growth and body composition of red porgy, Pagrus pagrus L.*}, author = {F M Rueda and F J Martinez and S Zamora and M Kentouri and P Divanach}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1046/j.1365-2109.1998.00228.x}, doi = {10.1046/j.1365-2109.1998.00228.x}, issn = {1355-557X, 1365-2109}, year = {1998}, date = {1998-06-01}, urldate = {2020-08-18}, journal = {Aquaculture Research}, volume = {29}, number = {6}, pages = {447--452}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Machias, A; Tsimenides, N; Kokokiris, L; Divanach, P Ring formation on otoliths and scales of Pagrus pagrus: A comparative study Journal Article Journal of Fish Biology, 52 , pp. 350 – 361, 1998. @article{machias_ring_1998, title = {Ring formation on otoliths and scales of Pagrus pagrus: A comparative study}, author = {A Machias and N Tsimenides and L Kokokiris and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0031891494&partnerID=40&md5=87566c6d1571076dbe0eb747e98a83cd}, year = {1998}, date = {1998-01-01}, journal = {Journal of Fish Biology}, volume = {52}, pages = {350 -- 361}, abstract = {One annulus formed on the scales of farmed red porgy Pagrus pagrus each year during March and one opaque zone on the otoliths during June. Scales proved to be more sensitive than otoliths in recording the life history of the red porgy. A false annulus (ring of capture) was observed on the scales of the 0+ age group, but not on the otoliths. A gradual lengthening of the period of annulus formation was observed, from the younger to the older fish. The annulus formation in fish older than 2+ was less synchronized, due to the aquaculture conditions. Scales were more accurate than otoliths in annuli formation. A high percentage of missing or additional opaque zones were observed on otoliths of older fish. These irregularities were related to fish maturation and/or farming conditions. Farmed fish grew three times as fast as the wild fish.}, keywords = {}, pubstate = {published}, tppubtype = {article} } One annulus formed on the scales of farmed red porgy Pagrus pagrus each year during March and one opaque zone on the otoliths during June. Scales proved to be more sensitive than otoliths in recording the life history of the red porgy. A false annulus (ring of capture) was observed on the scales of the 0+ age group, but not on the otoliths. A gradual lengthening of the period of annulus formation was observed, from the younger to the older fish. The annulus formation in fish older than 2+ was less synchronized, due to the aquaculture conditions. Scales were more accurate than otoliths in annuli formation. A high percentage of missing or additional opaque zones were observed on otoliths of older fish. These irregularities were related to fish maturation and/or farming conditions. Farmed fish grew three times as fast as the wild fish. |
Palma, J; Andrade, J P; Paspatis, M; Divanach, P; Kentouri, M Morphometric characters in gilthead sea bream, Sparus aurata, red porgy, Pagrus pagrus and their hybrids (Sparidae) Journal Article Italian Journal of Zoology, 65 (sup1), pp. 435 – 439, 1998, ISSN: 1125-0003, 1748-5851. @article{palma_morphometric_1998, title = {Morphometric characters in gilthead sea bream, Sparus aurata, red porgy, Pagrus pagrus and their hybrids (Sparidae)}, author = {J Palma and J P Andrade and M Paspatis and P Divanach and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0039702587&partnerID=40&md5=5ee992d3199c1a20e1e909920d20f6d8}, doi = {10.1080/11250009809386861}, issn = {1125-0003, 1748-5851}, year = {1998}, date = {1998-01-01}, journal = {Italian Journal of Zoology}, volume = {65}, number = {sup1}, pages = {435 -- 439}, abstract = {A morphometric comparison was made between Sparus aurata (n = 88), Pagrus pagrus (n = 38) and their hybrid 5. aurata (female) x P. pagrus (male) (n = 81) using 15 morphometric characteristics. The morphometric characteristics of the hybrids were generally intermediate to those of the parental species. However, characteristics related to the body shape (6) were more similar to S. aurata and those characters related to the head shape (9) were more similar to P. pagrus.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A morphometric comparison was made between Sparus aurata (n = 88), Pagrus pagrus (n = 38) and their hybrid 5. aurata (female) x P. pagrus (male) (n = 81) using 15 morphometric characteristics. The morphometric characteristics of the hybrids were generally intermediate to those of the parental species. However, characteristics related to the body shape (6) were more similar to S. aurata and those characters related to the head shape (9) were more similar to P. pagrus. |
1997 |
Koumoundouros, G; Oran, G; Divanach, P; Stefanakis, S; Kentouri, M The opercular complex deformity in intensive gilthead sea bream (Sparus aurata L.) larviculture. Moment of apparition and description Journal Article Aquaculture, 156 (1-2), pp. 165–177, 1997, ISSN: 00448486. @article{koumoundouros_opercular_1997, title = {The opercular complex deformity in intensive gilthead sea bream (Sparus aurata L.) larviculture. Moment of apparition and description}, author = {G Koumoundouros and G Oran and P Divanach and S Stefanakis and M Kentouri}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848697892940}, doi = {10.1016/S0044-8486(97)89294-0}, issn = {00448486}, year = {1997}, date = {1997-10-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {156}, number = {1-2}, pages = {165--177}, abstract = {External morphology, head morphometry and osteology were used for early detection, description and heuristic study of the opercular complex abnormalities of intensively reared gilthead sea bream larvae (Sparus aurata). The first evidence of malformations were observed in larvae of 6.1 mm total length (TL). The abnormalities were fully developed during metamorphosis (11.0-22.0 mm TL). i.e., before the end of the hatchery phase. In 81.4% of the abnormal fish, the problem was unilateral with similar right/left frequency. In 85.5% of the cases, it was associated with severe foldings and twists of the operculum and/or suboperculum and rarely with an atrophy of these bones. The etiology, aquaculture applications and research targets for the erasure of skeletal malformations are discussed.}, keywords = {}, pubstate = {published}, tppubtype = {article} } External morphology, head morphometry and osteology were used for early detection, description and heuristic study of the opercular complex abnormalities of intensively reared gilthead sea bream larvae (Sparus aurata). The first evidence of malformations were observed in larvae of 6.1 mm total length (TL). The abnormalities were fully developed during metamorphosis (11.0-22.0 mm TL). i.e., before the end of the hatchery phase. In 81.4% of the abnormal fish, the problem was unilateral with similar right/left frequency. In 85.5% of the cases, it was associated with severe foldings and twists of the operculum and/or suboperculum and rarely with an atrophy of these bones. The etiology, aquaculture applications and research targets for the erasure of skeletal malformations are discussed. |
Rueda, F M; Lopez, J A; Martinez, F J; Zamora, S; Divanach, P; Kentouri, M Fatty acids in muscle of wild and farmed red porgy, Pagrus pagrus Journal Article Aquaculture Nutrition, 3 (3), pp. 161–165, 1997, ISSN: 1353-5773, 1365-2095. @article{rueda_fatty_1997, title = {Fatty acids in muscle of wild and farmed red porgy, Pagrus pagrus}, author = {F M Rueda and J A Lopez and F J Martinez and S Zamora and P Divanach and M Kentouri}, url = {http://doi.wiley.com/10.1046/j.1365-2095.1997.00088.x}, doi = {10.1046/j.1365-2095.1997.00088.x}, issn = {1353-5773, 1365-2095}, year = {1997}, date = {1997-09-01}, urldate = {2020-08-18}, journal = {Aquaculture Nutrition}, volume = {3}, number = {3}, pages = {161--165}, abstract = {The total fat content and the fatty acids of total lipids (TL), phospholipids (PL) and triacylglycerols (TAG) were analysed in white muscle from wild and reared red porgy, Pagrus pagrus (L.). The fat percentage was higher in reared (3.03 ± 0.57%) (mean ± SE) than wild (0.65 ± 0.03%) fish. The fatty acid pattern of reared porgy TL reflected the diet’s lipid. Wild red porgy showed higher levels of ω3 and ω6 polyunsaturated fatty acids (PUFA) than reared, but both the atherogenic index and the index of thrombogenicity were very low for both wild and reared red porgy. The lipid fractions showed that the PL were composed of very high levels of PUFA, especially 22:6ω3, and their composition seemed to be better regulated than the composition of TAG. TAG showed greater differences between wild and reared fish, presumably because they reflected the dietary content of lipids. PUFA were the principal component of wild red porgy TAG.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The total fat content and the fatty acids of total lipids (TL), phospholipids (PL) and triacylglycerols (TAG) were analysed in white muscle from wild and reared red porgy, Pagrus pagrus (L.). The fat percentage was higher in reared (3.03 ± 0.57%) (mean ± SE) than wild (0.65 ± 0.03%) fish. The fatty acid pattern of reared porgy TL reflected the diet’s lipid. Wild red porgy showed higher levels of ω3 and ω6 polyunsaturated fatty acids (PUFA) than reared, but both the atherogenic index and the index of thrombogenicity were very low for both wild and reared red porgy. The lipid fractions showed that the PL were composed of very high levels of PUFA, especially 22:6ω3, and their composition seemed to be better regulated than the composition of TAG. TAG showed greater differences between wild and reared fish, presumably because they reflected the dietary content of lipids. PUFA were the principal component of wild red porgy TAG. |
Koumoundouros, G; Gagliardi, F; Divanach, P; Boglione, C; Cataudella, S; Kentouri, M Normal and abnormal osteological development of caudal fin in Sparus aurata L. fry Journal Article Aquaculture, 149 (3-4), pp. 215–226, 1997, ISSN: 00448486. @article{koumoundouros_normal_1997, title = {Normal and abnormal osteological development of caudal fin in Sparus aurata L. fry}, author = {G Koumoundouros and F Gagliardi and P Divanach and C Boglione and S Cataudella and M Kentouri}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848696014433}, doi = {10.1016/S0044-8486(96)01443-3}, issn = {00448486}, year = {1997}, date = {1997-03-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {149}, number = {3-4}, pages = {215--226}, abstract = {Normal osteological development of the caudal fin in gilthead sea bream is described at the larval and juvenile stages. It was used as a standard for the study of a sea bream larval population with characteristic osteological deviations which give rise to abnormal juveniles. Normal specimens were reared under extensive rearing conditions, and abnormal specimens under intensive rearing conditions. The first hypural elements appearing in the abnormal preflexion larvae were deformed and displaced. With development, all elements related to the caudal region (parhypural, hypuralia, epuralia, vertebra centra, neural and haemal processes) formed abnormally. The abnormalities were extra-numerous elements, fusions, deformities and displacements of the elements.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Normal osteological development of the caudal fin in gilthead sea bream is described at the larval and juvenile stages. It was used as a standard for the study of a sea bream larval population with characteristic osteological deviations which give rise to abnormal juveniles. Normal specimens were reared under extensive rearing conditions, and abnormal specimens under intensive rearing conditions. The first hypural elements appearing in the abnormal preflexion larvae were deformed and displaced. With development, all elements related to the caudal region (parhypural, hypuralia, epuralia, vertebra centra, neural and haemal processes) formed abnormally. The abnormalities were extra-numerous elements, fusions, deformities and displacements of the elements. |
Divanach, P; Papandroulakis, N; Anastasiadis, P; Koumoundouros, G; Kentouri, M Aquaculture, 156 , pp. 145 – 155, 1997. @article{divanach_effect_1997, title = {Effect of water currents on the development of skeletal deformities in sea bass (Dicentrarchus labtax L.) with functional swimbladder during postlarval and nursery phase}, author = {P Divanach and N Papandroulakis and P Anastasiadis and G Koumoundouros and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0030678081&partnerID=40&md5=ff2eb4edf54484b5987625d1168e5cac}, year = {1997}, date = {1997-01-01}, journal = {Aquaculture}, volume = {156}, pages = {145 -- 155}, abstract = {Lordosis in reared sea bass was previously related either to the absence of swimbladder, or to bad or late inflation of this organ when damages were irreversible. Our results prove that these explanations are insufficient and that similar deformations may occur frequently in fry with functional swimbladder during the early weaning phase, if currents in tanks are higher than 10 cm s-1.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Lordosis in reared sea bass was previously related either to the absence of swimbladder, or to bad or late inflation of this organ when damages were irreversible. Our results prove that these explanations are insufficient and that similar deformations may occur frequently in fry with functional swimbladder during the early weaning phase, if currents in tanks are higher than 10 cm s-1. |
Pitta, P; Giannakourou, A; Divanach, P; Kentouri, M Planktonic food web in marine mesocosms in the Eastern Mediterranean: Bottom-up or top-down regulation? Journal Article Hydrobiologia, 363 , pp. 97 – 105, 1997. @article{pitta_planktonic_1997, title = {Planktonic food web in marine mesocosms in the Eastern Mediterranean: Bottom-up or top-down regulation?}, author = {P Pitta and A Giannakourou and P Divanach and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0031402824&partnerID=40&md5=d32feeb1ddf43866aab11765538d6bfa}, year = {1997}, date = {1997-01-01}, journal = {Hydrobiologia}, volume = {363}, pages = {97 -- 105}, abstract = {A mesocosm experiment was conducted in order to study the structure of the planktonic food web. The dynamics of pico-, nano- and microplankton populations were followed during 40 days in four large (40 m3) enclosures. In three tanks a gradient of added nutrients (nitrogen and phosphorus) was applied, while a fourth tank was used as a control. On day 14, the top predator (sea bream Sparus aurata larvae) was introduced into the tanks and part of the water column in each tank was isolated in a plastic bag without fish larvae, to act as a control for predation. Physical parameters, chlorophyll a and nutrient concentrations, as well as plankton concentrations were monitored. A diatom bloom was observed in all four tanks, in the first phase ending with silicate depletion. Flagellate and dinoflagellate abundance subsequently increased, these organisms being limited by zooplankton grazing. The zooplankton populations were controlled by both resources (mostly flagellates) and predation (by fish larvae) as indicated by the results of the control experiments.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A mesocosm experiment was conducted in order to study the structure of the planktonic food web. The dynamics of pico-, nano- and microplankton populations were followed during 40 days in four large (40 m3) enclosures. In three tanks a gradient of added nutrients (nitrogen and phosphorus) was applied, while a fourth tank was used as a control. On day 14, the top predator (sea bream Sparus aurata larvae) was introduced into the tanks and part of the water column in each tank was isolated in a plastic bag without fish larvae, to act as a control for predation. Physical parameters, chlorophyll a and nutrient concentrations, as well as plankton concentrations were monitored. A diatom bloom was observed in all four tanks, in the first phase ending with silicate depletion. Flagellate and dinoflagellate abundance subsequently increased, these organisms being limited by zooplankton grazing. The zooplankton populations were controlled by both resources (mostly flagellates) and predation (by fish larvae) as indicated by the results of the control experiments. |
Pavlidis, M; Berry, M; Divanach, P; Kentouri, M Diel pattern of haematocrit, serum metabolites, osmotic pressure, electrolytes and thyroid hormones in sea bass and sea bream Journal Article Aquaculture International, 5 , pp. 237 – 247, 1997. @article{pavlidis_diel_1997, title = {Diel pattern of haematocrit, serum metabolites, osmotic pressure, electrolytes and thyroid hormones in sea bass and sea bream}, author = {M Pavlidis and M Berry and P Divanach and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0000527603&partnerID=40&md5=43ffd0cedb64e4f7359a2e7f9a22c7ef}, year = {1997}, date = {1997-01-01}, journal = {Aquaculture International}, volume = {5}, pages = {237 -- 247}, abstract = {The diel pattern of haematocrit, serum metabolites, electrolytes and thyroid hormones are described in sea bass, Dicentrarchus labrax, and sea bream, Sparus auratus, held under 12L:12D light cycle (0600-1800 h light). No diel rhythmicity in haematocrit, total proteins, potassium and chloride was observed. Maximum values of glucose, osmotic pressure and sodium were recorded at dusk (1800 h) in both species, irrespective of differences in the size of fish and feeding regime used. Maximum and minimum triiodothyronine values were found during the scotophase in bass and during the photophase in bream. Maximum thyroxine levels occurred at 1400 h (bass) and 1800 h (bream) and minimum during the scotophase, in both species. The light/dark alternation seems to be an important synchronizer of the observed diel changes. Results are discussed in relation to the timing of feeding and the general activity of the fish.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The diel pattern of haematocrit, serum metabolites, electrolytes and thyroid hormones are described in sea bass, Dicentrarchus labrax, and sea bream, Sparus auratus, held under 12L:12D light cycle (0600-1800 h light). No diel rhythmicity in haematocrit, total proteins, potassium and chloride was observed. Maximum values of glucose, osmotic pressure and sodium were recorded at dusk (1800 h) in both species, irrespective of differences in the size of fish and feeding regime used. Maximum and minimum triiodothyronine values were found during the scotophase in bass and during the photophase in bream. Maximum thyroxine levels occurred at 1400 h (bass) and 1800 h (bream) and minimum during the scotophase, in both species. The light/dark alternation seems to be an important synchronizer of the observed diel changes. Results are discussed in relation to the timing of feeding and the general activity of the fish. |
1996 |
Boujard, T; Jourdan, M; Kentouri, M; Divanach, P Diel feeding activity and the effect of time-restricted self-feeding on growth and feed conversion in European sea bass Journal Article Aquaculture, 139 , pp. 117 – 127, 1996. @article{boujard_diel_1996, title = {Diel feeding activity and the effect of time-restricted self-feeding on growth and feed conversion in European sea bass}, author = {T Boujard and M Jourdan and M Kentouri and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0029667241&partnerID=40&md5=4f928f95721eadc7b9602a9134a4da65}, year = {1996}, date = {1996-01-01}, journal = {Aquaculture}, volume = {139}, pages = {117 -- 127}, abstract = {The feeding rhythm and growth performance of European sea bass Dicentrarchus labrax (initial weight 65 g ) were studied under free and time-restricted access to self-feeders. Under free access to the feeders, significant feeding rhythms with a nocturnal acrophase were observed, although there was considerable variability between replicates. When access to the self-feeders was restricted to two periods of 4 h day-1, the feed demand (FD) was reduced at the beginning of the experiment but not at the end, indicating that the period of adaptation, rather than appetite, was affected by such feeding practice. When access to the self-feeders was restricted to only one period of 4 h day-1, two thirds of the replicates eventually displayed FD similar to the fish having free access to feed, indicating that the restricted temporal access to feed could be compensated for. There was no detectable effect of feeding protocol on feed conversion or body composition, but growth was closely related to the total FD. Thus, growth was influenced by the fact that fish subjected to a time-restricted access to feed needed time to adapt to the new feeding situation. The results indicate that sea bass are capable of operating self-feeders, and can easily modify their feeding, resulting in both good growth performance and good feed conversion. © 1996 Elsevier Science B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The feeding rhythm and growth performance of European sea bass Dicentrarchus labrax (initial weight 65 g ) were studied under free and time-restricted access to self-feeders. Under free access to the feeders, significant feeding rhythms with a nocturnal acrophase were observed, although there was considerable variability between replicates. When access to the self-feeders was restricted to two periods of 4 h day-1, the feed demand (FD) was reduced at the beginning of the experiment but not at the end, indicating that the period of adaptation, rather than appetite, was affected by such feeding practice. When access to the self-feeders was restricted to only one period of 4 h day-1, two thirds of the replicates eventually displayed FD similar to the fish having free access to feed, indicating that the restricted temporal access to feed could be compensated for. There was no detectable effect of feeding protocol on feed conversion or body composition, but growth was closely related to the total FD. Thus, growth was influenced by the fact that fish subjected to a time-restricted access to feed needed time to adapt to the new feeding situation. The results indicate that sea bass are capable of operating self-feeders, and can easily modify their feeding, resulting in both good growth performance and good feed conversion. © 1996 Elsevier Science B.V. All rights reserved. |
1995 |
Koumoundouros, G; Kiriakos, Z; Divanach, P; Kentouri, M Morphometric relationships as criteria for the evaluation of larval quality of gilthead sea bream Journal Article Aquaculture International, 3 , pp. 143 – 149, 1995. @article{koumoundouros_morphometric_1995, title = {Morphometric relationships as criteria for the evaluation of larval quality of gilthead sea bream}, author = {G Koumoundouros and Z Kiriakos and P Divanach and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0001396165&partnerID=40&md5=37c948592073f3aa8dc5432b1793f12d}, year = {1995}, date = {1995-01-01}, journal = {Aquaculture International}, volume = {3}, pages = {143 -- 149}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Pitta, P; Divanach, P; Kentouri, M Nano- and microplankton in Iraklion Bay, Crete: comparison of coastal waters and mesocosm enclosures assemblages Inproceedings Biology and ecology of shallow coastal waters. Proc. 28th European marine biology symposium, Iraklio, Crete, 1993, pp. 79 – 86, Iraklio, Crete, 1995, (Biology and ecology of shallow coastal waters. Proc. 28th European marine biology symposium, Iraklio, Crete, 1993). @inproceedings{pitta_nano-_1995, title = {Nano- and microplankton in Iraklion Bay, Crete: comparison of coastal waters and mesocosm enclosures assemblages}, author = {P Pitta and P Divanach and M Kentouri}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0029548756&partnerID=40&md5=8cdd28ffb2242355883c69f908cffab9}, year = {1995}, date = {1995-01-01}, booktitle = {Biology and ecology of shallow coastal waters. Proc. 28th European marine biology symposium, Iraklio, Crete, 1993}, pages = {79 -- 86}, address = {Iraklio, Crete}, abstract = {The impacts of different nutrient levels were studied using a combination of a) in situ sampling at 1 m depth from a coastal area, and b) the monitoring of three mesocosms (60 m3). In the first mesocosm sea water only was enclosed, while in the other two, varying quantities of nutrients were added, forming an artificial eutrophication gradient. The increase in nutrient concentration results in an acceleration of the successional stages in comparison with the natural environment. Enclosure conditions lead to increased phytoplankton blooms and dominance of the assemblages by a few species. Significant quantitative and qualitative differences were observed in both the chlorophyll concentrations and the structure and evolution of assemblages between those from the wild and those from the enclosures, irrespective of whether or not they had been fertilized with nutrients.}, note = {Biology and ecology of shallow coastal waters. Proc. 28th European marine biology symposium, Iraklio, Crete, 1993}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } The impacts of different nutrient levels were studied using a combination of a) in situ sampling at 1 m depth from a coastal area, and b) the monitoring of three mesocosms (60 m3). In the first mesocosm sea water only was enclosed, while in the other two, varying quantities of nutrients were added, forming an artificial eutrophication gradient. The increase in nutrient concentration results in an acceleration of the successional stages in comparison with the natural environment. Enclosure conditions lead to increased phytoplankton blooms and dominance of the assemblages by a few species. Significant quantitative and qualitative differences were observed in both the chlorophyll concentrations and the structure and evolution of assemblages between those from the wild and those from the enclosures, irrespective of whether or not they had been fertilized with nutrients. |
1994 |
Efthimiou, Stefan; Divanach, Pascal; Rosenthal, Harald Growth, food conversion and agonistic behaviour in common dentex (Dentex dentex) juveniles fed on pelleted moist and dry diets Journal Article Aquatic Living Resources, 7 (4), pp. 267–275, 1994, ISSN: 0990-7440. @article{efthimiou_growth_1994, title = {Growth, food conversion and agonistic behaviour in common dentex (Dentex dentex) juveniles fed on pelleted moist and dry diets}, author = {Stefan Efthimiou and Pascal Divanach and Harald Rosenthal}, url = {http://www.alr-journal.org/10.1051/alr:1994029}, doi = {10.1051/alr:1994029}, issn = {0990-7440}, year = {1994}, date = {1994-10-01}, urldate = {2020-08-18}, journal = {Aquatic Living Resources}, volume = {7}, number = {4}, pages = {267--275}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Kentouri, M; Leon, L; Tort, L; Divanach, P Experimental methodology in aquaculture: Modification of the feeding rate of the gilthead sea bream Sparus aurata at self-feeder after weighing Journal Article Aquaculture, 119 , pp. 191 – 200, 1994. @article{kentouri_experimental_1994, title = {Experimental methodology in aquaculture: Modification of the feeding rate of the gilthead sea bream Sparus aurata at self-feeder after weighing}, author = {M Kentouri and L Leon and L Tort and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0028162232&partnerID=40&md5=0d0b264671f7229e9b6e3706db194cfb}, year = {1994}, date = {1994-01-01}, journal = {Aquaculture}, volume = {119}, pages = {191 -- 200}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Kentouri, M; O’Neill, D; Divanach, P; Charalambakis, G Aquaculture & Fisheries Management, 25 , pp. 741 – 752, 1994. @article{kentouri_study_1994, title = {A study on the quantitative water requirements of red porgies, Pagrus pagrus L. (Pisces: Sparidae), during early on-growing under self-feeding conditions}, author = {M Kentouri and D O’Neill and P Divanach and G Charalambakis}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0027949634&partnerID=40&md5=661e8d1bc25aa61cecc404500cd25aa9}, year = {1994}, date = {1994-01-01}, journal = {Aquaculture & Fisheries Management}, volume = {25}, pages = {741 -- 752}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
1993 |
Kentouri, M; Anthouard, M; Divanach, P Response to Colours in Dicentrarchus labrax (Moronidae), in a Self‐Feeding and Multiple Choice Condition Journal Article Marine Ecology, 14 (3), pp. 259–274, 1993, ISSN: 0173-9565, 1439-0485, (Orignal title (in French): La Réponse aux Couleurs chez Dicentrarchus labrax (Moronidae), Placé en Situation de «Self‐feeding», et de Choix Multiple). @article{kentouri_response_1993, title = {Response to Colours in Dicentrarchus labrax (Moronidae), in a Self‐Feeding and Multiple Choice Condition}, author = {M Kentouri and M Anthouard and P Divanach}, url = {http://doi.wiley.com/10.1111/j.1439-0485.1993.tb00483.x}, doi = {10.1111/j.1439-0485.1993.tb00483.x}, issn = {0173-9565, 1439-0485}, year = {1993}, date = {1993-09-01}, urldate = {2020-08-18}, journal = {Marine Ecology}, volume = {14}, number = {3}, pages = {259--274}, note = {Orignal title (in French): La Réponse aux Couleurs chez Dicentrarchus labrax (Moronidae), Placé en Situation de «Self‐feeding», et de Choix Multiple}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
1988 |
Hidalgo, F; Kentouri, M; Divanach, P The utilisation of a self-feeder as a tool for the nutritional study of sea bass, Dicentrarchus labrax — Preliminary results with methionine Journal Article Aquaculture, 68 (2), pp. 177–190, 1988, ISSN: 00448486, (Original title (in French): Sur l'utilisation du self feeder comme outil d'epreuve nutritionnelle du loup, Dicentrarchus labrax — Résultats préliminaires avec la méthionine). @article{hidalgo_utilisation_1988, title = {The utilisation of a self-feeder as a tool for the nutritional study of sea bass, Dicentrarchus labrax — Preliminary results with methionine}, author = {F Hidalgo and M Kentouri and P Divanach}, url = {https://linkinghub.elsevier.com/retrieve/pii/0044848688902402}, doi = {10.1016/0044-8486(88)90240-2}, issn = {00448486}, year = {1988}, date = {1988-02-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {68}, number = {2}, pages = {177--190}, abstract = {Five different diets were offered simultaneously to each of three different groups of fish under self feeding conditions. Diets differed only in methionine content (0.30, 0.65, 1.00, 1.35 and 1.70% of diet). Fish groups differed in weight (group A, 0.2 g; group B, 2.0 g; group C, 200 g mean weight). Measure of food demand showed that group A refused feeding and declined in vitality and health; group B showed a net preference for 1.35% methionine (considered as optimum); group C was more influenced by spatial preferences. In a second experimental period, the following modifications were introduced: commercial food for sea bass (Aqualim dry pellet) was offered to group A in addition to the five initial diets; for groups B and C changes in spatial distribution of self feeders were introduced. The fish in group A reacted instantly to the introduction of the commercial food which they ate preferentially. Fish of groups B and C maintained behaviour similar to that of the previous period. The authors conclude that sea bass can detect and discriminate small doses of methionine, showing preferences easily demonstrable with the self-feeding method. © 1988.}, note = {Original title (in French): Sur l'utilisation du self feeder comme outil d'epreuve nutritionnelle du loup, Dicentrarchus labrax — Résultats préliminaires avec la méthionine}, keywords = {}, pubstate = {published}, tppubtype = {article} } Five different diets were offered simultaneously to each of three different groups of fish under self feeding conditions. Diets differed only in methionine content (0.30, 0.65, 1.00, 1.35 and 1.70% of diet). Fish groups differed in weight (group A, 0.2 g; group B, 2.0 g; group C, 200 g mean weight). Measure of food demand showed that group A refused feeding and declined in vitality and health; group B showed a net preference for 1.35% methionine (considered as optimum); group C was more influenced by spatial preferences. In a second experimental period, the following modifications were introduced: commercial food for sea bass (Aqualim dry pellet) was offered to group A in addition to the five initial diets; for groups B and C changes in spatial distribution of self feeders were introduced. The fish in group A reacted instantly to the introduction of the commercial food which they ate preferentially. Fish of groups B and C maintained behaviour similar to that of the previous period. The authors conclude that sea bass can detect and discriminate small doses of methionine, showing preferences easily demonstrable with the self-feeding method. © 1988. |
1987 |
Divanach, P; Kentouri, M; Cavalier, F Larval trophic scheme of Diplodus sargus and ichthyoplanktonic productivity of coastal lagoons in Languedoc-Roussillon, France Journal Article Acta Oecologia/Oecologia Applicata, 8 , pp. 105–110, 1987, (Original title (in French): Organisation du reseau trophique larvaire de Diplodus sargus et productivite ichtyoplanctonique des milieux laguno-cotiers en Languedoc-Roussillon). @article{divanach_larval_1987, title = {Larval trophic scheme of Diplodus sargus and ichthyoplanktonic productivity of coastal lagoons in Languedoc-Roussillon, France}, author = {P Divanach and M Kentouri and F Cavalier}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0023556970&partnerID=40&md5=30ca1a61ab6f5173f09dedccf4415b2d}, year = {1987}, date = {1987-01-01}, journal = {Acta Oecologia/Oecologia Applicata}, volume = {8}, pages = {105--110}, abstract = {Successive planktonic blooms (Skeletonema; small ciliates; Strobilidium and Favella; copepods) and benthic blooms led to a productivity almost 100 time higher than natural sea, due to a lack of predators and competitors. -from English summary}, note = {Original title (in French): Organisation du reseau trophique larvaire de Diplodus sargus et productivite ichtyoplanctonique des milieux laguno-cotiers en Languedoc-Roussillon}, keywords = {}, pubstate = {published}, tppubtype = {article} } Successive planktonic blooms (Skeletonema; small ciliates; Strobilidium and Favella; copepods) and benthic blooms led to a productivity almost 100 time higher than natural sea, due to a lack of predators and competitors. -from English summary |
1986 |
Divanach, P; Kentouri, M; Dewavrin, G Aquaculture, 52 (1), pp. 21–29, 1986, ISSN: 00448486, (Original title (in French): Sur le sevrage et l'evolution des performances biologiques d'alevins de daurades, Sparus auratus, provenant d'elevage extensif, apres remplacement des nourrisseurs en continu par des distributeurs libre service). @article{divanach_weaning_1986, title = {The weaning and the development of biological performance of extensively reared sea bream, Sparus auratus, fry after replacing continuous feeders by self-feeding distributors}, author = {P Divanach and M Kentouri and G Dewavrin}, url = {https://linkinghub.elsevier.com/retrieve/pii/0044848686901031}, doi = {10.1016/0044-8486(86)90103-1}, issn = {00448486}, year = {1986}, date = {1986-02-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {52}, number = {1}, pages = {21--29}, abstract = {Extensively reared 0.13 g Sparus auratus sea bream fry were placed under intensive conditions (one individual per litre) and progressively weaned with dry pellets by two successive methods: prescribed feeding, and self-feeding. Results show that biological and ecological data on fry productivity are strongly dependent on feeding strategy. During the first period when pellets were supplied with a 9 h/day continuous feeder, production data are technically and economically correct but biologically poor (food conversion ratio 5 to 7; chronic mortality of 0.77% per day; disastrous hydric conversion ratio of 2415 m3/kg production). On the other hand, during the second period when the fry were self-feeding and free to regulate their feeding pattern, biological data become very good (food conversion ratio 1.95; mortality less than 0.03% day; hydric conversion ratio of 249 m3/kg production). The two techniques are discussed and compared in their ecological, metabolic and physiological aspects. © 1986.}, note = {Original title (in French): Sur le sevrage et l'evolution des performances biologiques d'alevins de daurades, Sparus auratus, provenant d'elevage extensif, apres remplacement des nourrisseurs en continu par des distributeurs libre service}, keywords = {}, pubstate = {published}, tppubtype = {article} } Extensively reared 0.13 g Sparus auratus sea bream fry were placed under intensive conditions (one individual per litre) and progressively weaned with dry pellets by two successive methods: prescribed feeding, and self-feeding. Results show that biological and ecological data on fry productivity are strongly dependent on feeding strategy. During the first period when pellets were supplied with a 9 h/day continuous feeder, production data are technically and economically correct but biologically poor (food conversion ratio 5 to 7; chronic mortality of 0.77% per day; disastrous hydric conversion ratio of 2415 m3/kg production). On the other hand, during the second period when the fry were self-feeding and free to regulate their feeding pattern, biological data become very good (food conversion ratio 1.95; mortality less than 0.03% day; hydric conversion ratio of 249 m3/kg production). The two techniques are discussed and compared in their ecological, metabolic and physiological aspects. © 1986. |
Kentouri, M; Divanach, P; Batique, O; Anthouard, M Aquaculture, 52 (2), pp. 117–124, 1986, ISSN: 00448486, (Original title (in French): Role des individus conditionnes dans l'initiation a l'auto-nourrissage et dans l'adaptation a la captivite du loup, Dicentrarchus labrax, 0+ sauvage, en periode hivernale). @article{kentouri_influence_1986, title = {Influence of preconditioned individuals in the initiation of self-feeding and in adaptation to captivity of wild 0+ sea-bass, Dicentrarchus labrax, in winter}, author = {M Kentouri and P Divanach and O Batique and M Anthouard}, url = {https://linkinghub.elsevier.com/retrieve/pii/0044848686900311}, doi = {10.1016/0044-8486(86)90031-1}, issn = {00448486}, year = {1986}, date = {1986-02-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {52}, number = {2}, pages = {117--124}, abstract = {It is shown that the presence of pre-conditioned fish in a wild naive group reared under limiting ecological conditions (winter) facilitates the adaptation of the group to captivity by accelerating the learning of self-feeding and minimizing rearing stress and mortality. The reasons for this phenomenon, which has implications for aquaculture and fishing, are discussed and related to the learning capacity of this species when imitating pre-conditioned fish. © 1986.}, note = {Original title (in French): Role des individus conditionnes dans l'initiation a l'auto-nourrissage et dans l'adaptation a la captivite du loup, Dicentrarchus labrax, 0+ sauvage, en periode hivernale}, keywords = {}, pubstate = {published}, tppubtype = {article} } It is shown that the presence of pre-conditioned fish in a wild naive group reared under limiting ecological conditions (winter) facilitates the adaptation of the group to captivity by accelerating the learning of self-feeding and minimizing rearing stress and mortality. The reasons for this phenomenon, which has implications for aquaculture and fishing, are discussed and related to the learning capacity of this species when imitating pre-conditioned fish. © 1986. |
Kentouri, M; Divanach, P Food ranges of sparid larvae in controlled conditions. Specific selectivity of Sparus aurata. Journal Article Oceanologica Acta, 9 , pp. 343–348, 1986, (Original title (in French): Spectres alimentaires des larves de sparides en conditions controlees. Selectivite specifique de la daurade Sparus aurata.). @article{kentouri_food_1986, title = {Food ranges of sparid larvae in controlled conditions. Specific selectivity of Sparus aurata.}, author = {M Kentouri and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0022832257&partnerID=40&md5=2a720f7edf8cbd1e0c7f2ebd247d9df3}, year = {1986}, date = {1986-01-01}, journal = {Oceanologica Acta}, volume = {9}, pages = {343--348}, abstract = {Up to 5.5 mm total length, larvae actively choose Synchaeta triophthalma, then prefer Eurytemora velox. -from English summary}, note = {Original title (in French): Spectres alimentaires des larves de sparides en conditions controlees. Selectivite specifique de la daurade Sparus aurata.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Up to 5.5 mm total length, larvae actively choose Synchaeta triophthalma, then prefer Eurytemora velox. -from English summary |
1983 |
Divanach, P; Kentouri, M Preliminary data on production techniques, growth, and survival of thognathus mormyrus larvae Journal Article Aquaculture, 31 (2-4), pp. 245–256, 1983, ISSN: 00448486, (Original title (in French): Donnees preliminaires sur la technique de production, la croissance et la survie des larves de marbre Lithognathus mormyrus). @article{divanach_preliminary_1983, title = {Preliminary data on production techniques, growth, and survival of thognathus mormyrus larvae}, author = {P Divanach and M Kentouri}, url = {https://linkinghub.elsevier.com/retrieve/pii/0044848683903162}, doi = {10.1016/0044-8486(83)90316-2}, issn = {00448486}, year = {1983}, date = {1983-03-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {31}, number = {2-4}, pages = {245--256}, abstract = {Eggs of Lithognathus mormyrus stripped from dead breeding fish are incubated and give larvae which are then reared to the fry stage. The yield of eggs depends on the freshness of the breeding fish. Survival of larvae is made possible by a natural bloom of ciliates, the duration of which depends on low initial larval density. In spite of a length of only 1.7 mm at hatching, growth is comparable with that of the sea bass. Fry adaptation to pellets is easy. Possibilities of mass production of fry with extensive rearing techniques are discussed. © 1983.}, note = {Original title (in French): Donnees preliminaires sur la technique de production, la croissance et la survie des larves de marbre Lithognathus mormyrus}, keywords = {}, pubstate = {published}, tppubtype = {article} } Eggs of Lithognathus mormyrus stripped from dead breeding fish are incubated and give larvae which are then reared to the fry stage. The yield of eggs depends on the freshness of the breeding fish. Survival of larvae is made possible by a natural bloom of ciliates, the duration of which depends on low initial larval density. In spite of a length of only 1.7 mm at hatching, growth is comparable with that of the sea bass. Fry adaptation to pellets is easy. Possibilities of mass production of fry with extensive rearing techniques are discussed. © 1983. |
Divanach, P; Kentouri, M; Paris, J Research on possible crops for aquaculture in Artemia salina lagoons, Diplodus sargus. Journal Article Comptes Rendus des Seances - Academie des Sciences, Serie III, 296 , pp. 29–31, 1983, (Original title (in French): Recherches sur la production exploitable au niveau aquacole dans des lagunes a Artemia salina.). @article{divanach_research_1983, title = {Research on possible crops for aquaculture in Artemia salina lagoons, Diplodus sargus.}, author = {P Divanach and M Kentouri and J Paris}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0021062507&partnerID=40&md5=fef95aada51fd20d5a6a74629b061876}, year = {1983}, date = {1983-01-01}, journal = {Comptes Rendus des Seances - Academie des Sciences, Serie III}, volume = {296}, pages = {29--31}, abstract = {Harvesting of 2 hyper haline 1750 m 2 lagoons during Artemia bloom gave crops of 839 and 2365 kg/ha, depending on fishing gear. When Artemia were used as exclusive food for Diplodus sargus fry in intensive rearing with 30-100% body weight ration/day, Artemia growth was 0.3-0.4 mm/day, mortality was <2%/month and conversion ration was 9-9.6. -English summary}, note = {Original title (in French): Recherches sur la production exploitable au niveau aquacole dans des lagunes a Artemia salina.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Harvesting of 2 hyper haline 1750 m 2 lagoons during Artemia bloom gave crops of 839 and 2365 kg/ha, depending on fishing gear. When Artemia were used as exclusive food for Diplodus sargus fry in intensive rearing with 30-100% body weight ration/day, Artemia growth was 0.3-0.4 mm/day, mortality was <2%/month and conversion ration was 9-9.6. -English summary |
Kentouri, M; Divanach, P Contribution to the knowledge of behaviour and biology of striped sea bream Lithognathus mormyrus larvae (Sparidae) in rearing conditions. Journal Article Annales de Zootechnie, 32 (2), pp. 135 – 152, 1983, (Original title (in French): Contribution a la connaissance du comportement et de la biologie des larves de marbre Lithognathus mormyrus (Sparides) en elevage). @article{kentouri_contribution_1983, title = {Contribution to the knowledge of behaviour and biology of striped sea bream Lithognathus mormyrus larvae (Sparidae) in rearing conditions.}, author = {M Kentouri and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0020863476&partnerID=40&md5=50946d4bcccb9f11d0e7f3c322daa7fc}, year = {1983}, date = {1983-01-01}, journal = {Annales de Zootechnie}, volume = {32}, number = {2}, pages = {135 -- 152}, abstract = {Feeding was always diversified, but mainly composed of 4 types of prey: from 2.9-2.5 mm, ciliates and veligers; from 4-7 mm, rotifers, up to 7 mm, crustacea. When ciliates were not included in the ration, the early trophic phase was disturbed. Growth was low, mortality very high and survivors often entered a critical stage. After a supra normal period of prospection, activity and feeding decreased, the larvae exhibited prelarval rest postures and then died.-from Sport Fishery Abstracts}, note = {Original title (in French): Contribution a la connaissance du comportement et de la biologie des larves de marbre Lithognathus mormyrus (Sparides) en elevage}, keywords = {}, pubstate = {published}, tppubtype = {article} } Feeding was always diversified, but mainly composed of 4 types of prey: from 2.9-2.5 mm, ciliates and veligers; from 4-7 mm, rotifers, up to 7 mm, crustacea. When ciliates were not included in the ration, the early trophic phase was disturbed. Growth was low, mortality very high and survivors often entered a critical stage. After a supra normal period of prospection, activity and feeding decreased, the larvae exhibited prelarval rest postures and then died.-from Sport Fishery Abstracts |
Divanach, P; Kentouri, M Aquaculture, 35 , pp. 43–55, 1983, ISSN: 00448486, (Original title (in French): Influence des conditions trophiques initiales sur la resorption des reserves lipidiques, la croissance et la survie des larves de daurades, Sparus auratus, en elevage extensif). @article{divanach_influence_1983, title = {The influence of initial trophic conditions on oil globule resorption, growth and survival of gilt head sea bream, Sparus auratus, larvae in extensive breeding}, author = {P Divanach and M Kentouri}, url = {https://linkinghub.elsevier.com/retrieve/pii/0044848683900698}, doi = {10.1016/0044-8486(83)90069-8}, issn = {00448486}, year = {1983}, date = {1983-01-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {35}, pages = {43--55}, abstract = {Seven trials are described in which larval gilt head sea bream, Sparus auratus, was reared in extensive conditions. Food was provided initially from an endogenous plankton bloom and then, as grazing increased to high levels, from an exogenous supply. Immediate rapid growth, high survival rates, slow oil globule resorption and easy weaning with exogenous plankton were obtained with a diet of pelagic marine ciliates and/or the rotifer, Synchaeta triophthalma. With Branchionus plicatilis or copepod nauplii, results were poor and close to those obtained in the unfed trial. There was a high mortality, slow growth, quick oil globule resorption and the survivors suffered a critical phase. With a mixture of mussel veligers and Synchaeta littoralis, feeding results were intermediate. The reasons for success or failure are discussed and predictions are made for the development of a rearing programme. © 1983.}, note = {Original title (in French): Influence des conditions trophiques initiales sur la resorption des reserves lipidiques, la croissance et la survie des larves de daurades, Sparus auratus, en elevage extensif}, keywords = {}, pubstate = {published}, tppubtype = {article} } Seven trials are described in which larval gilt head sea bream, Sparus auratus, was reared in extensive conditions. Food was provided initially from an endogenous plankton bloom and then, as grazing increased to high levels, from an exogenous supply. Immediate rapid growth, high survival rates, slow oil globule resorption and easy weaning with exogenous plankton were obtained with a diet of pelagic marine ciliates and/or the rotifer, Synchaeta triophthalma. With Branchionus plicatilis or copepod nauplii, results were poor and close to those obtained in the unfed trial. There was a high mortality, slow growth, quick oil globule resorption and the survivors suffered a critical phase. With a mixture of mussel veligers and Synchaeta littoralis, feeding results were intermediate. The reasons for success or failure are discussed and predictions are made for the development of a rearing programme. © 1983. |
1982 |
Divanach, P; Kentouri, M; Paris, J Stages of the embryonic and larval development of the white sea bream, Diplodus sargus L., in rearing conditions Journal Article Aquaculture, 27 (4), pp. 339–353, 1982, ISSN: 00448486, (Original title (in French): Etapes du developpement embryonnaire et larvaire du sar, Diplodus sargus L., en elevage). @article{divanach_stages_1982, title = {Stages of the embryonic and larval development of the white sea bream, Diplodus sargus L., in rearing conditions}, author = {P Divanach and M Kentouri and J Paris}, url = {https://linkinghub.elsevier.com/retrieve/pii/0044848682901223}, doi = {10.1016/0044-8486(82)90122-3}, issn = {00448486}, year = {1982}, date = {1982-05-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {27}, number = {4}, pages = {339--353}, abstract = {Eggs of white sea bream (Diplodus sargus), taken from wild parents, were incubated and, after hatching, produced larvae which were then reared up to the fry stage. The different stages of development during the embryonic and larval periods are described. Some characteristics of the eggs (buoyancy, duration of incubation, critical periods) and of the larvae (metrical characters) are reported. © 1982.}, note = {Original title (in French): Etapes du developpement embryonnaire et larvaire du sar, Diplodus sargus L., en elevage}, keywords = {}, pubstate = {published}, tppubtype = {article} } Eggs of white sea bream (Diplodus sargus), taken from wild parents, were incubated and, after hatching, produced larvae which were then reared up to the fry stage. The different stages of development during the embryonic and larval periods are described. Some characteristics of the eggs (buoyancy, duration of incubation, critical periods) and of the larvae (metrical characters) are reported. © 1982. |
Kentouri, M; Divanach, P Aquaculture, 27 , pp. 355 – 376, 1982. @article{kentouri_differences_1982, title = {Differences and similarities in the genesis of the locomotor and trophic behaviour of the prelarval stages of Sparus auratus, Diplodus vulgaris and Diplodus sargus}, author = {M Kentouri and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0020220374&partnerID=40&md5=ca25fa5468ce2d956d97645d687678ad}, year = {1982}, date = {1982-01-01}, journal = {Aquaculture}, volume = {27}, pages = {355 -- 376}, abstract = {The behaviour of the prelarval stages of Sparus auratus, Diplodus vulgaris and Diplodus sargus was studied in 570-l tanks at a temperature of 19°C. The different stages in swimming from hatching to the age of 4 days are as follows; period of dominating immobility (1st day); passive vertical migration (2nd day); first oblique swimming (3rd day); first horizontal swimming (4th day). The different speeds of swimming and the distances covered are calculated; the specific differences are mentioned. The predation training period is described and food selectivity at this stage is demonstrated. Aquacultural implications are discussed. © 1982.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The behaviour of the prelarval stages of Sparus auratus, Diplodus vulgaris and Diplodus sargus was studied in 570-l tanks at a temperature of 19°C. The different stages in swimming from hatching to the age of 4 days are as follows; period of dominating immobility (1st day); passive vertical migration (2nd day); first oblique swimming (3rd day); first horizontal swimming (4th day). The different speeds of swimming and the distances covered are calculated; the specific differences are mentioned. The predation training period is described and food selectivity at this stage is demonstrated. Aquacultural implications are discussed. © 1982. |
Kentouri, M; Divanach, P Comptes Rendus des Seances, Academie des Sciences, Serie III, 294 (17), pp. 859 – 861, 1982, (Original title (in French): Comportement et regime alimentaire des larves de marbre Lithognathus mormyrus (Poisson, Teleost., Sparidae) elevees dans des conditions de choix trophique polyspecifique et pluridimensionnel.). @article{kentouri_behaviour_1982, title = {Behaviour and prey selection of striped sea bream Lithognathus mormyrus ( Sparidae) larvae reared in polyspecific and pluridimensional food conditions.}, author = {M Kentouri and P Divanach}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0020401751&partnerID=40&md5=77c3818f6f7c57ac0f66fe44d712b5ea}, year = {1982}, date = {1982-01-01}, journal = {Comptes Rendus des Seances, Academie des Sciences, Serie III}, volume = {294}, number = {17}, pages = {859 -- 861}, abstract = {Striped sea bream larvae select, in order of preference, ciliata and veligers (2.9-3.5mm), rotifers (4-7mm), small pelagic crustacea up to 7mm. Digestibility of each prey class is very different. Veligers, barnacles and the rotifer Cephalodella remain alive in the gut.-from English summary}, note = {Original title (in French): Comportement et regime alimentaire des larves de marbre Lithognathus mormyrus (Poisson, Teleost., Sparidae) elevees dans des conditions de choix trophique polyspecifique et pluridimensionnel.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Striped sea bream larvae select, in order of preference, ciliata and veligers (2.9-3.5mm), rotifers (4-7mm), small pelagic crustacea up to 7mm. Digestibility of each prey class is very different. Veligers, barnacles and the rotifer Cephalodella remain alive in the gut.-from English summary |
1980 |
Kentouri, M; Divanach, P; Cantou, M Preliminary data in behavior, growth and survival of reared sea bream Diplodus sargus L. Journal Article Mediterranean Studies Review - General Fisheries Council, 57 , pp. 33–51, 1980, (Original title (in French): Donnees preliminaires sur le comportement, la croissance et la survie du sar Diplodus sargus L. en elevage). @article{kentouri_preliminary_1980, title = {Preliminary data in behavior, growth and survival of reared sea bream Diplodus sargus L.}, author = {M Kentouri and P Divanach and M Cantou}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0019237286&partnerID=40&md5=0f8ae0457204d44480d1358711cdee9e}, year = {1980}, date = {1980-01-01}, journal = {Mediterranean Studies Review - General Fisheries Council}, volume = {57}, pages = {33--51}, abstract = {For larvae, the survival rate ranges from 3-8% after 54 days and rises to 77% for juveniles after 14 months’ farming. Growth is fast during summer but drops from September to May. The specimens reach 34 g in the 1st year, and 123 g by the end of 17 months of culture.-from Sport Fishery Abstracts}, note = {Original title (in French): Donnees preliminaires sur le comportement, la croissance et la survie du sar Diplodus sargus L. en elevage}, keywords = {}, pubstate = {published}, tppubtype = {article} } For larvae, the survival rate ranges from 3-8% after 54 days and rises to 77% for juveniles after 14 months’ farming. Growth is fast during summer but drops from September to May. The specimens reach 34 g in the 1st year, and 123 g by the end of 17 months of culture.-from Sport Fishery Abstracts |