Efthimia (Efi) Cotou

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Personal InformationEfthimia (Efi) Cotou
Principal Researcher
Biomarkers & Bioassays

HCMR 46.7 km of Athens – Sounio Ave. 19013 Anavissos Attica, Greece

0030 2291076492

ecotou@hcmr.gr

Field & DegreeAquatic Toxicology, PhD
SectionAquaculture
LocationAnavissos
Mobile Number0030 6977785760
ORCID iDhttps://orcid.org/0000-0003-1480-8257
ResearchGate IDhttps://www.researchgate.net/profile/Efthimia_Cotou
Scientific impact
Publication recordNo of ProjectsThesis supervision
H-index (scopus): 13Coordinator: 3
Articles: 27Princ. Invest.: 6MSc: 4
Citations-total: 565Project member: 15BSc: 4
Citations-nonself: 551Funding: 647,671
Education & training
Year (completed) Degree, Department, University, Town (optional), Country
2010Methods in Micro-Nanotechnology and Nano-Biotechnology, National Centre for Scientific Research "Demokritos", Athens, Greece (funded by FT7-People-INT-2008)
2008DNA Basic Sequencing and Fragment Analysis, Applied Biosystems, Manchester, UK
2006Genetically modified organisms: Detection of genetic material with PCR, Department of Biology, University of Athens, Greece
1995Intensive training course on "The applications of ecotoxicology in the monitoring and assessment of marine pollution', Institute of General Physiology, University of Genova, Italy (funded by UNEP/MAP)
1993PhD in Aquatic Toxicology & Environmental Sanitation, University of Ghent, Belgium (Granted by EEC-87400682BEPUA). PhD Thesis Title: Controlled production of ephippia of Daphnia pulex (Leydig) as biological material to start toxicity tests on chemicals
1986BSc in Biology, University of Athens, Greece. BSc Thesis Title: Study the toxicity of an oil dispersant on the Na-K and Mg ATPase system of Artemia
Work Experience
Year (start) Position – Organization, Town (optional), Country
2014Senior Researcher, Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, Athens, Greece
2007Associate Researcher, Institute of Aquaculture, Hellenic Centre for Marine Research, Athens, Greece
2006Research Scientist, Institute of Aquaculture, Hellenic Centre for Marine Research, Athens, Greece
1994Research Scientist, Institute of Oceanography, National Centre for Marine Research, Athens, Greece
1986Research Assistant, Division of Zoology, Department of Biology, National and Kapodistrian University of Athens, Athens, Greece
Personal skills
Mother tongue(s)Greek
Other language(s)
Language Basic/Intermediate/Proficient
EnglishProficient
GermanIntermediate
DutchBasic
Computer skills Windows environment, Microsoft Office, SPSS
Other skills Scuba diving, Taekwondo
Educational activities
Teaching experience
  • 2006-2014Lecturer on “Prevention, Therapy and Toxicity in Aquatic Environment” (MSc level: Aquaculture - Pathological Problems of Aquatic Cultured Organisms), Veterinary School of University of Thessaly, Greece.
  • 2003-2006: Lecturer on Physico-Chemistry (BSc level), Department of Physico-Chemistry and Technology of Materials, Technical University of Piraeus, Greece.
  • 1991-1992Laboratory Assistant on “Rapid and Cost-effective bioassays”, Lab of Biological Research in Aquatic Pollution, University of Ghent, Belgium.
  • 1990-1993Laboratory Assistant on Ecotoxicology (MSc level), Lab of Biological Research in Aquatic Pollution, University of Ghent, Belgium.
  • 1987: Laboratory Assistant on Aquaculture (BSc degree), Department of Biology, University of Athens, Greece.
Supervision
MSc

Garpousis Konstantinos 2020-2021. Integrated MultiTrophic Aquaculture-Exploitation of co-culture bivalves and finfish, Agricultural University of Athens.

Zervou Niki, 2012-2013. Thesis Title: Effects of fish feeds composition on hepatic biotransformation of oxolinic acid and flumequine in Sparus aurata, Department of Biochemistry and Biotechnology, University of Thessaly.

Alexiou Ioannis, 2011-2012. Thesis Title: Comparative study of expression of CYP19A2 aromatase and CYP1A2 in gibel carp (Carassius gibelio) from Lake Karla and Lake Pamvotis. Veterinary School, University of Thessaly.

Fragouli Chrisoula, 2010-2011. Thesis Title: The effects of fish oil and plant oils on drug-metabolizing and antioxidants enzymes activities in Sparus aurata treated with oxolinic acid. Veterinary School, University of Thessaly

BSc

Liapi Aspasia 2020-2021. Chemical composition of mussels and oysters co-cultured in finfish and shellfish parks, Agricultural University of Athens.

Loukanari Theodora, 2015-2016: Thesis Title: Sequencing CYP27A1 gene of Argyrosomus regius and study its hepatic gene expression after administration of several vitamin D3 levels during juveniles’ development. Department of Animal Science and Aquaculture, Agricultural University of Athens.

Tsourou Olga, 2014-2015: Thesis Title: The effect of fish feeds composition on biotransformation and detoxification enzymes in Sparus aurata administered orally with potentiated sulfa. Department of Biology, University of Athens.

Koutsodimou Maria, 2003-2004. Thesis Title: Study the toxicity of suspended and re-suspended particulate matter in marine benthic organisms, Department of Biology, University of Athens.

Practicals

Paias Christos, 2021. Activities of digestive and amtioxidant enzymes in fish fed on alternative diets, Aristotle University of Athens.

Avgeri Feggula, 2018. Department of Animal Science and Aquaculture, Agricultural University of Athens. Project Title: Integrated Multi-Trophic Aquaculture (fish, filter and deposit feeders) in an open production system: Effects on growth, proximate composition of organisms and impacts in the environment.

Christaki Dimitra, 2017. Department of Environmental Technology, Technological Educational Institute of Ionian Islands. Project title: Integrated Multi-Trophic Co-Cultivation of European sea bass, filter- & deposit-feeders: Growth performance & Environmental quality. 

Kamateri Georgia, 2014. Department of Ichthyology and Aquatic Environment, University of Thessaly. Project title: Biotoxicity and sublethal effects of leguminous ingredients in seabass .

Katsoula Christina, 2014. Department of Ichthyology and Aquatic Environment, University of Thessaly. Project title: Biotoxicity and sublethal effects of leguminous ingredients in seabass .

Antoniou Vasiliki, 2011. Division of Zoology, Department of Biology, National and Kapodistrian University of Athens. Project title: Endocrine disruptors in fish. Division of Zoology, Department of Biology, University of Athens 

Giavrouta Labrini, 2009. Department of Aquaculture & Fishery, Technological Educational Institute of Messologhi. Project Title: Toxicity of aqua feeds ingredients. 

Conferences-Symposia

International and National Congesses in total 38 and 9, respectively

International Congresses (peer reviewed)

  1. Van Den Vel A., Cotou and L. Lambert, 1989. The use of neonates hatched from ephippia in toxicity tests with Daphnia pulex. Abstract book of SETAC-USA, 10th Annual Meeting, Toronto Canada.
  2. Cotou E. and G. Persoone, 1991. Ephippia of Daphnia pulex as a standardized source of organisms for toxicity testing. Abstract book of SETAC-Europe, 1st Annual Meeting, Sheffield UK (funded by EEC).
  3. Cotou E., Roussis V., Rapti T. & C. Vagias, 1998. A comparative study on the metallothionein content of six marine benthic organisms. Comm. Int. Mer Medit., 35: 246-247.
  4. Tsangaris C., Cotou E. and E. Papathanassiou, 2000. Combined physiological and biochemical measurements for the assessment of pollution in Amvrakikos Gulf. Proc. 5th Confer. Environ. Pollution, A. Anagnostopoulos (ed), Thessaloniki, Greece, 233-310.
  5. Tsangaris C., Cotou and E. Papathanassiou, 2001. Multiple biomarker assessment for marine pollution: a case study to distinguish the type of pollutants in Amvrakikos gulf (Greece). Abstract book of PRIMO 11, Plymouth, UK.
  6. Catsiki VA., Cotou E., Strogyloudi E., Tsangaris C. and E. Papathanassiou 2001. Application of various biological methodologies in order to characterize the state of pollution of the coastal environment of Amvrakikos Gulf (Greece). 4th International Marine Environmenta Modelling Conference, 4-6 October, Athens, Greece.
  7. Cotou E., M.A. Pancussi-Papadopoulou and I. Hatzianestis. 2002. Microtox Solid-phase for marine sediments: A case study in Nestos river region (North Aegean Sea). Proc. VI Int. Conf. on Protection & Restoration of the Environment, 346-350.
  8. Cotou E., M. Koutsodimou and A. Nikolaidou, 2004. Biochemical biomarkers responses to suspended and re-suspended particles in situ. Rapp. Comm. Int. Mer Medit., 37: 183.
  9. Cotou E., H. Kaberi, A. Papageorgiou and C. Tsangaris, 2005. Potential toxicity of ferrous-nickel slag after sandblasting operations. 12th International Symposium on toxicity assessment Skiathos 12-17 June.
  10. Cotou E., P. Anastasiadou and K. Machera, 2006. PCP treated timbers in a coastal area of Greece: Environmental impact and biological effects. Protection and Restoration of the Environment VIII, Chania Greece 3-7 July.
  11. Varo I., Navarro J.C., Del Ramo J., Cotou E., Torreblanca A. 2007. A proteomic approach for identifying new biomarkers of toxicity in marine fish: Effects of copper sulphate treatments on gilthead seabream (Sparus aurata). Abstract Book in SETAC Europe 17th Annual meeting in Porto, 20-24 May 2007.
  12. Varo I., Cotou E., Navarro J.C., Del Ramo J., Torreblanca A., 2007. A proteomic approach for identifying new biomarkers of toxicity in marine fish: Effects of ivermectin treatments on gilthead seabream (Sparus aurata). Abstract Book in SETAC Europe 17th Annual meeting in Porto, 20-24 May 2007.
  13. Varo I., Rodriquez C., Navaro J.C., Martivez I., Cotou E., Acosta N.G., Del Ramo J., Diaz-Mayans J., Torreblanca A., 2007. A conceptual framework for the application of biotechnology to detect signals of global warming in fish. Abstract Book in SETAC Europe 17th Annual meeting in Porto, 20-24 May 2007.
  14. Tsangaris C., Fountoulaki E., Cotou E., Nizheradze K. 2008. Oxidative stress biomarkers in grey mullet (Mugil cephalus) from polluted Greek coastal waters. Abstract Book on the 26th European Conference “Health implications of Environmental contamination”, 31st March-2nd April 2008, Athens, Greece.
  15. Rigos, G., Henry, M., Cotou E., Papoutsi, E., Zonaras, V., Nikoloudaki, , Nikolopoulou, D., Nengas, I., Alexis, M., 2009. The effects of diet composition (plant Vs Fish-oil-based diets) on plasma concentration of various antibacterials in gilthead sea bream, Sparus aurata. 14th EAFP international conference, Diseases of fish and shellfish, Praga, Czech 14-19 September, p.408.
  16. Rigos G., Zonaras V., Papoutsi E, Henry M., Cotou E., Nikoloudaki X, Vasilaki A., Nengas I., Alexis M., 2010. The Effect of Diet composition on oxytetracycline availability in gilthead bream Sparus aurata at two water temperatures. Aquaculture Europe 2010, European Aquaculture Society. Porto, Portugal, October 5-8, 2010, page 1104.
  17. Cotou E., Tsangaris C., Henry M., 2010. Comparative study of biochemical and immunological biomarkers in three marine bivalve mollusks exposed in the field. Abstract Book on the 2nd European Conference “Health Implications of Environmental Contamination”, 26th - 29st September 2010, Myconos, Greece page 54. ISBN 978-960-6865-30-5
  18. Fountoulaki E., Vasilaki A., Henry M., Cotou E., Rigos G., Sweetman J., Nengas I., 2011. Selenium supplementation in commercial type diets for sea bream (Sparus aurata) : Effects on growth, tissue concentration and immune status. Abstract Book of “Mediterranean Aquaculture 2020” on Aquaculture Europe 2011, 18th – 21st October 2011 in Rhodos Greece, page 357.
  19. Rigos G., Cotou E., Fountoulaki H., Nikoloudaki X., Vasilaki A., Arvanitis P., Nengas I., Katharios P., 2011. Preliminary investigation of the antiparasitic effects of orally-administered quinine against natural exoparasitic infections in gilthead sea bream (Sparus aurata). Abstract Book of “Mediterranean Aquaculture 2020” on Aquaculture Europe 2011, 18th – 21st October 2011 in Rhodos Greece, page 901.
  20. Cotou E., Henry M., 2011. Effects of copper based antifouling coated nets on selected biomarkers of sea bream (Sparus aurata) in laboratory experiments. Abstract Book of “Mediterranean Aquaculture 2020” on Aquaculture Europe 2011, 18th – 21st October 2011 in Rhodos, Greece, page 206.
  21. Kokou F., Cotou E., Rigos G., Kentouri M., Alexis M., 2012. Modulation of antioxidant enzyme activities by dietary soy bean meal in gilthead sea bream (Sparus aurata). WAS, Aquaculture America. Las Vegas, USA, Fed 29-2 March 2012.
  22. Fountoulaki E., Rigos G., Cotou E., Nikoloudaki Ch. Nengas I., 2012. Evaluation of different levels of caprylic acid in feeds for sea bream Sparus aurata: Preliminary results on tissue concentration. WAS, Aquaculture America. Las Vegas, USA, Fed 29-2 March 2012.
  23. Rigos G., Fountoulaki E., Dourala N., Zarkadas I., Karacostas I., Dotsika E., Nikoloudaki C.,Cotou E., 2013. Tissue distribution and field evaluation of caprylic acid against natural infections of Sparicotyle chrysophrii in acge-reared gilthead sea bream Sparus aurata. 16th EAFP International conference on diseases of fish and shellfish, Tampere, September 2-6, 2013.
  24. Cotou E, Fountoulaki E, Kokou F, Karalazos V., 2014. Effects of dietary interventions on glutathione content and activity of glutathione-related enzymes in European sea bass (Dicentrarchus labrax). EAS Adding Value, Aquaculture Europe 2014, 14-17 October Donostia-San Sebastian, Spain.
  25. Cotou E. Kokou F., Rigos G., 2014. Differential effects of orally administered quinine on detoxifying and antioxidant enzymes activities of parasitized sea bream Sparus aurata. EAS Adding Value, Aquaculture Europe 2014, 14-17 October Donostia-San Sebastian, Spain.
  26. Katharios P., Bastias R., Kokkari C., Vasilakis M., Cotou E., Fountoulaki E., Rigos G., 2014. Surplus of dietary micronutrients against dermal erosions associated with bacterial infections in European sea bass (Dicentrarchus labrax). EAS Adding Value, Aquaculture Europe 2014, 14-17 October Donostia-San Sebastian, Spain
  27. Kyriazis I.D., Fountoulaki E., Rigos G., Toubanaki D.K., Cotou E., Dourala N., Karacostas I., Κaragouni Ε., Dotsika E., 2014. Dietary administration a caprylic acid in cage-reared gilthead sea bream Sparus aurata reduces natural infections of Microcotyle spp by modulating immune-relevant genes. HydroMedit 2014, 1st International Congress of Applied Ichthyology & Aquatic Environment 13-15 November 2014, Volos, Greece
  28. Cotou E., Fountoulaki E., Hager-Theodorides A.L., Theodorou G., Tsertou M., Loukanari T., Katharios P., Kounna Ch., 2016. The effects of dietary inclusions of vitamin D3 associated to CYP27A1, antioxidant enzymes and non-infectious systemic granulomatosis in meagre (Argyrosomus regius). International Symposium of Fish Nutrition and Feeding 6-10 June 2016 Idaho USA pp. 107.
  29. Fountoulaki E., Cotou E., Vasilaki A., Nikoloudaki C., Kokou F., Rigos G., Karalazos V., 2016. Evaluation of dietary micronutrient supplementation in low fish meal diets for juvenile European sea bass (Dicentrarchus labrax). International Symposium of Fish Nutrition and Feeding 6-10 June 2016 Idaho USA pp. 407.
  30. Cotou E., Fountoulaki Ε., Henry Μ., 2016. Screening the toxicity of “alternative” fish feeds and fish fecal waste with a rapid, cost effective biotest. Aquaculture Europe, 20-23 September 2016, Edinburgh, Scotland pp 227-228.
  31. Fountoulaki E., Cotou E., Vasilaki A., Nikoloudaki C., Kokou F., Rigos G., Karalazos V., 2016. Evaluation of dietary micronutrient supplementation in low fish meal diets for juvenile European sea bass (Dicentrarchus labrax). International Symposium of Fish Nutrition and Feeding 6-10 June 2016 Idaho USA pp. 407.
  32. Fountoulaki Ε., Kogiannou D., Vasilaki A., Nikoloudaki Ch., Pyrenis G. Rigos G., Henry M., Kounna C., Cotou E., 2016. Micronutrient supplementation affects growth and health status of cultured amberjack (Seriola dumerili). Aquaculture Europe, 20-23 September 2016, Edinburgh, Scotland pp 341-342.
  33. Cotou E., Kotsiri M., Mitrizakis N., Papandroulakis N., 2017. Advanced detection of digestive enzymes in greater amberjack (Seriola dumerili) at early life stages. Aquaculture Europe, 17-20 October 2017, Dubrovnic, Croatia pp. 237-238.
  34. Cotou E., Androni A., Fountoulaki E., Rigos G., Konstadinopoulou A., Assimakopoulou G., Pyrenis G., Christaki D., 2017. Integrated multi-trophic co-cultivation of European sea bass, filter- & deposit-feeders: I– Preliminary results on growth performance and environmental quality. Aquaculture Europe, 17-20 October 2017, Dubrovnic, Croatia pp. 239-240.
  35. Aubin J., Lefebvre S., Callier M.C., Wilfart A., Roque d’Orbcastel E., Cunha M.E., Ferreira H., Gamito S., Mortillaro J.M., Caruso D., Cotou E., Fountoulaki E., Nahon S., Metaxa I., Miliou H., 2017. Efficiency and Environmental Performance of IMTA in Marine and Freshwater Systems. Aquaculture Europe, 17-20 October 2017, Dubrovnic, Croatia pp. 43-44.
  36. Tsertou M.I., Chatzifotis S., Fontanillas R., Cotou E., Fountoulaki E., Smyrli M., Antonopoulou E., Katharios P., 2017. Recent advances in the study of systemic granulomatosis in meagre (Argyrosomus regius). Aquaculture Europe, 17-20 October 2017, Dubrovnic, Croatia pp. 1168-1169.
  37. Cotou E., Fountoulaki E., Avgeri F., Kotsiri M, Alexandratos A., Henry M., Kogiannou D., Rigos G., Androni A., Konstadinopoulou A., Assimakopoulou G., 2018. Availability and environmental effects of commercial feeds (fish meal vs plant meal based) & fish feed waste in an IMTA system of fish, filter- & deposit- feeders: A Multi-Indicator Assessment Approach. WAS/EAS 2018, August 25-29, Montpellier, France.
  38. Kotsiri M., Cotou E., Fountoulaki E., Charalabous K., 2019. Effects of heat-treated soy & sunflower flour for fish meal substitution on energy reserves and antioxidant & digestive enzymes in the gilthead sea bream (Sparus aurata). Aquaculture Europe 2019, October 7-10, Berlin, Germany.
  39. Cotou E., Halabalaki M., Henry M., Vasilaki A., Politakis N., Kotsiri M., Fountoulaki E., Nengas I., 2021. Heat-treated soybean meal: Effects on dietary isoflavone genistein content and Sparus aurata juveniles growth indexes, immune, antioxidant, lipogenic & digestive system. Aquaculture Europe 2021, October 4-7, Madeira, Portugal.

 National Congesses (peer reviewed) 

  1. Cotou E., Bei F. and E. Papathanassiou, 1997. Scope for Growth & Bioaccumulation of Trace Metals in Mytilus galloprovincialis of Kalloni Gulf. Proc.5th Hel. Symp. Oceanogr. & Fish, 1: 113-116
  2. Cotou E., 2000. The use of luminescent bacteria to characterize chemical stress in marine organisms. Proc. 6th Hel. Symp. Oceanogr. & Fish, 1: 366-370.
  3. Cotou Ε., Kaberi Η., Sakellariou D., 2003. Detection of hormesis effects in environmental samples of Korinthiakos Gulf. 7th Hel. Symp. Oceanogr. & Fish, 6-9 May 2003, Hersonissos, Grete.
  4. Tsangaris A., Lyssimachou A., Strogyloudi E., Cotou E., Papathanassiou E., 2006. Effects of endocrine disruptors on sex steroid hormones in Mytilus galloprovincialis: Preliminary results. Proceedings of the 8th Symposium of Oceanography and Fisheries, Thessaloniki 4-8 June 2006: 103.
  5. Cotou E., Henry M., Rigos G., Alexis M., 2009. Cellular responses of the farmed sea bass Dicentrarchus labrax to copper oxide antifoulants. Proceedings of the 9th Symposium of Oceanography and Fisheries Volume II pp. 1297-1302.
  6. Henry M., Nikoloudaki Ch., Vasilaki A., Cotou E., Fountoulaki E., 2013. Evaluation of fish feeds with various protein/lipid rations for the finest status of Umbrina cirrosa, L., immune system. 15th Panhellenic Congress of Ichthyologists, Thessaloniki 10-13 October 2013, pp. 435-438.
  7. Cotou E., Fountoulaki E., Varvarigos, P., 2015. Evaluation of faba bean-induced toxigenic pathology in European sea bass. Proceeding of the 11th Symposium of Oceanography and Fisheries 13-17 May 2015 Mytilene Greece pp. 273-276.
  8. Tsourou O., Cotou E., Megalofonou P., 2015. Effects of fish feeds composition on the hepatic metabolizing enzymes of sulfadiazine in the gilthead sea bream Sparus aurata. Proceedings of the 37th Scientific Conference of Hellenic Association for Biological Sciences, Volos, May 21-23, p. 413.
  9. Fountoulaki E., Cotou E., Henry M., Grigorakis K., Kogiannou D., Kotsiri M., Vassilaki A., Nikoloudaki Ch., Pyrenis, G., Sweetman J., Nengas I., 2018. Benefits of organic vs inorganic minerals on growth, health and product quality of the European sea bass (Dicentrarchus labrax). Book of Abstracts for the 12th Symposium of Oceanography and Fisheries 30 May-3 June 2018 Corfu Island, Greece page 28.
Services

- Toxicity Bioassays

- Prognostic Biomarkers

 

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