Ioannis (Yannis) Kotzamanis

@article{dritsas_investigating_2025,

title = {Investigating the Potential of Newly Isolated Microalgae Strains from the Ionian Sea (Greece) Cultured in an Open Raceway Pond},

author = {Panagiotis Dritsas and Stefania Patsialou and Dimitrios Kampantais and Efstratios Roussos and Yannis Kotzamanis and Athanasia Tekerlekopoulou and Dimitris V Vayenas and George Aggelis},

url = {https://www.mdpi.com/2076-3417/15/12/6680

/wp-content/uploads/2025/07/2025-Dritsas-AppSci-35.pdf},

doi = {10.3390/app15126680},

issn = {2076-3417},

year  = {2025},

date = {2025-07-07},

urldate = {2025-07-10},

journal = {Applied Sciences},

volume = {15},

number = {12},

pages = {6680},

abstract = {Microalgae are a subject of interest not only for fundamental research but for various biotechnological applications as well. In this study, the ability of newly isolated strains, i.e., Picochlorum costavermella VAS2.5, Picochlorum oklahomense SAG4.4, Microchloropsis gaditana VON5.3, and Nephroselmis pyriformis PAT2.7, to grow when cultured in an open raceway pond under laboratory conditions and produce various metabolites of high-added value was evaluated. N. pyriformis PAT2.7 and P. costavermella VAS2.5 were the greatest in biomass production (exceeding 0.4 g/L), while P. costavermella VAS2.5 and M. gaditana VON5.3 were the greatest in lipid production (reaching approximately 18%, wt/wt). The lipid fraction of glycolipids and sphingolipids was predominant (43.6–55.4%, wt/wt), followed by neutral lipids (27.1–50.1%, wt/wt) and phospholipids (6.9–17.4%, wt/wt). Picochlora and M. gaditana VON5.3 lipids were rich in Δ5,8,11,14,17C20:5 and/or Δ9,12,15C18:3, while N. pyriformis PAT2.7 synthesized Δ9C16:1 in large quantities (30–40%, wt/wt). All strains showed remarkable yields in polysaccharide and protein production, demonstrated a well-balanced amino acid profile, and synthesized pigments in amounts comparable to other studies. The biochemical profiles of these strains showcased their suitability for use primarily in the aquaculture sector.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{wei_dietary_2025,

title = {Dietary iron oxide (Fe2O3) nanoparticles modulate growth performance, body composition, mineral content and intestinal health of yellow catfish juveniles (Pelteobagrus fulvidraco)},

author = {Xiao-Lei Wei and Zhi-Wei Hao and Yannis P Kotzamanis and Tian-Hua Zhang and Zhi-Bo Liu and Hong Yang and Zhi Luo},

url = {https://linkinghub.elsevier.com/retrieve/pii/S2352513425001255

/wp-content/uploads/2025/03/2025-Wei-AquaReports-16.pdf},

doi = {10.1016/j.aqrep.2025.102739},

issn = {23525134},

year  = {2025},

date = {2025-03-31},

urldate = {2025-03-31},

journal = {Aquaculture Reports},

volume = {42},

pages = {102739},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{roussos_status_2024,

title = {Status of Fishery Discards and By-Products in Greece and Potential Valorization Scenarios towards a National Exploitation Master Plan},

author = {Efstratios Roussos and George Triantaphyllidis and Vassiliki Ilia and Konstantinos Tsagarakis and Athanasios Machias and Leto-Aikaterini Tziveleka and Vassilios Roussis and Efstathia Ioannou and Yannis Kotzamanis},

url = {https://imbbc.hcmr.gr/wp-content/uploads/2024/06/2024-Roussos-Marinedrugs-28.pdf

https://www.mdpi.com/1660-3397/22/6/264},

doi = {10.3390/md22060264},

issn = {1660-3397},

year  = {2024},

date = {2024-06-17},

urldate = {2024-06-17},

journal = {Marine Drugs},

volume = {22},

number = {6},

pages = {264},

abstract = {The valorization of aquaculture/fishery processing by-products, as well as unavoidable/unwanted catches and discards in Greece, is currently an underutilized activity despite the fact that there are several best practices in Northern Europe and overseas. One of the main challenges is to determine whether the available quantities for processing are sufficient to warrant the valorization of discards and fish side streams. This is the first attempt to systematically record and analyze the available quantities of fish by-products and discards in Greece spatially and temporally in an effort to create a national exploitation Master Plan for the valorization of this unavoidable and unwanted biomass. A thorough survey conducted within the VIOAXIOPIO project unveiled a substantial biomass of around 19,000 tonnes annually that could be harnessed for valorization. Furthermore, the production of various High-Added-Value Biomolecules (HAVBs) was investigated and experimental trials were conducted to assess the potential yields, with the collected data used to formulate four valorization scenarios.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{tzortzatos_dietary_2024,

title = {Dietary Artemisia arborescens Supplementation Effects on Growth, Oxidative Status, and Immunity of Gilthead Seabream (Sparus aurata L.)},

author = {Odysseas-Panagiotis Tzortzatos and Dimitra K Toubanaki and Markos N Kolygas and Yannis Kotzamanis and Efstratios Roussos and Vasileios Bakopoulos and Achilleas Chatzopoulos and Fotini Athanassopoulou and Evdokia Karagouni},

url = {https://imbbc.hcmr.gr/wp-content/uploads/2024/05/2024-Tzortzatos-ANIMALS-24.pdf

https://www.mdpi.com/2076-2615/14/8/1161},

doi = {10.3390/ani14081161},

issn = {2076-2615},

year  = {2024},

date = {2024-04-29},

urldate = {2024-05-13},

journal = {Animals},

volume = {14},

number = {8},

pages = {1161},

abstract = {Fish infectious diseases are one of the main constraints of the aquaculture sector. The use of medicinal plants provides a sustainable way of protection using safe, eco-friendly compounds in a more cost-effective way of treatment, compared to antibiotics. The aim of the present study is the assessment of Artemisia arborescens (AA) feed-supplementation effects on gilthead seabream (Sparus aurata). Fish with an average initial body weight of 109.43 ± 3.81 g, were divided into two groups based on AA feed composition (A25 and A50). Following two months of ad libitum feeding, the effect of diets on fish weight and length were measured. Fish serum and mucus were analyzed for non-specific immune parameters (nitric oxide, lysozyme, myeloperoxidase, protease-/anti-protease activity, and complement), antibody responses, oxidative stress (cytochrome P450 1A1, metallothionein), and metabolism markers (total protein, alkaline phosphatase, and glucose). Expression levels of antioxidants (sod1, gpx1), cytokines (il-1b, il-10, tfgb1, and tnfa), hepcidin, and heat shock protein grp75 genes were measured in spleen samples. A results analysis indicated that A. arborescens use as a feed supplement has a compromised positive effect on the growth performance, immune response, and blood parameters of gilthead seabream. Overall, the suitability of A. arborescens as an efficient food supplement for gilthead seabream health improvement was investigated, setting the basis for its application assessment in Mediterranean aquaculture.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{zhao_effects_2024,

title = {Effects of dietary citric acid on growth performance, mineral status, body and muscle composition, muscle growth and mTOR signaling in yellow catfish Pelteobagrus fulvidraco fed with low-manganese diets},

author = {Tao Zhao and Jie-Jie Xu and Yannis P Kotzamanis and Dian-Guang Zhang and Yi-Chuang Xu and Hua Zheng and Ya-Kang Han and Zhi Luo},

url = {https://imbbc.hcmr.gr/wp-content/uploads/2024/03/2024-Kotzamanis-et-al-AQUA-16.pdf

https://linkinghub.elsevier.com/retrieve/pii/S0044848624000309},

doi = {10.1016/j.aquaculture.2024.740569},

issn = {00448486},

year  = {2024},

date = {2024-03-22},

urldate = {2024-03-22},

journal = {Aquaculture},

volume = {582},

pages = {740569},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{zhao_mno2_2024,

title = {MnO2 nanoparticles trigger hepatic lipotoxicity and mitophagy via mtROS-dependent Hsf1Ser326 phosphorylation},

author = {Tao Zhao and Hua Zheng and Jie-Jie Xu and Kostas Pantopoulos and Yi-Chuang Xu and Lu-Lu Liu and Xi-Jun Lei and Yannis P Kotzamanis and Zhi Luo},

url = {https://imbbc.hcmr.gr/wp-content/uploads/2024/03/2024-Kotzamanis-et-al-pre-print-FRBM-15.pdf

https://linkinghub.elsevier.com/retrieve/pii/S0891584923011383},

doi = {10.1016/j.freeradbiomed.2023.11.037},

issn = {08915849},

year  = {2024},

date = {2024-03-19},

urldate = {2024-03-19},

journal = {Free Radical Biology and Medicine},

volume = {210},

pages = {390--405},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{rotter_identification_2023,

title = {Identification of Marine Biotechnology Value Chains with High Potential in the Northern Mediterranean Region},

author = {Ana Rotter and Antonia Giannakourou and Jesús E Argente García and Grazia Marina Quero and Charlène Auregan and George Triantaphyllidis and Amalia Venetsanopoulou and Roberta De Carolis and Chrysa Efstratiou and Marina Aboal and María Ángeles Esteban Abad and Ernesta Grigalionyte-Bembič and Yannis Kotzamanis and Mate Kovač and Maja Ljubić Čmelar and Gian Marco Luna and Cristóbal Aguilera and Francisco Gabriel Acién Fernández and Juan Luis Gómez Pinchetti and Sonia Manzo and Iva Milašinčić and Antun Nadarmija and Luisa Parrella and Massimiliano Pinat and Efstratios Roussos and Colin Ruel and Elisabetta Salvatori and Francisco Javier Sánchez Vázquez and María Semitiel García and Antonio F Skarmeta Gómez and Jan Ulčar and Cristian Chiavetta},

url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/09/2023-Rotter-MarDrugs-50.pdf

https://www.mdpi.com/1660-3397/21/7/416},

doi = {10.3390/md21070416},

issn = {1660-3397},

year  = {2023},

date = {2023-09-25},

urldate = {2023-09-27},

journal = {Marine Drugs},

volume = {21},

number = {7},

pages = {416},

abstract = {Marine (blue) biotechnology is an emerging field enabling the valorization of new products and processes with massive potential for innovation and economic growth. In the Mediterranean region, this innovation potential is not exploited as well as in other European regions due to a lack of a clear identification of the different value chains and the high fragmentation of business innovation initiatives. As a result, several opportunities to create an innovative society are being missed. To address this problem, eight Northern Mediterranean countries (Croatia, France, Greece, Italy, Montenegro, Portugal, Slovenia and Spain) established five national blue biotechnology hubs to identify and address the bottlenecks that prevent the development of marine biotechnology in the region. Following a three-step approach (1. Analysis: setting the scene; 2. Transfer: identification of promising value chains; 3. Capitalization: community creation), we identified the three value chains that are most promising for the Northern Mediterranean region: algae production for added-value compounds, integrated multi-trophic aquaculture (IMTA) and valorization aquaculture/fisheries/processing by-products, unavoidable/unwanted catches and discards. The potential for the development and the technical and non-technical skills that are necessary to advance in this exciting field were identified through several stakeholder events which provided valuable insight and feedback that should be addressed for marine biotechnology in the Northern Mediterranean region to reach its full potential.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{mallioris_modulation_2022,

title = {Modulation of intestinal health and hepatic vacuolation in gilthead sea bream (Sparus aurata) juveniles by a mixture of dietary esterified butyrins, emulsifiers from plants and yeast extracts at low and high fish meal inclusion},

author = {Panagiotis Mallioris and Yannis Kotzamanis and Sofia Vardali and Efstratios Roussos and Vasiliki Ilia and Elissavet Paschali and Dimitrios Kampantais and Dimitrios Bouziotis and Alireza Khadem and Ioannis N Vatsos},

url = {https://linkinghub.elsevier.com/retrieve/pii/S0377840121003801},

doi = {10.1016/j.anifeedsci.2021.115194},

issn = {03778401},

year  = {2022},

date = {2022-10-26},

urldate = {2022-10-26},

journal = {Animal Feed Science and Technology},

volume = {284},

pages = {115194},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{mastoraki_insect_2022,

title = {Insect meals in feeds for juvenile gilthead seabream (Sparus aurata): Effects on growth, blood chemistry, hepatic metabolic enzymes, body composition and nutrient utilization},

author = {Maria Mastoraki and Lydia Katsika and Paula Enes and Inês Guerreiro and Yannis P Kotzamanis and Laura Gasco and Stavros Chatzifotis and Efthimia Antonopoulou},

url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/10/2022-Mastoraki-AQUApre-print-70.pdf

https://linkinghub.elsevier.com/retrieve/pii/S0044848622007918},

doi = {10.1016/j.aquaculture.2022.738674},

issn = {00448486},

year  = {2022},

date = {2022-10-14},

urldate = {2022-10-14},

journal = {Aquaculture},

volume = {561},

pages = {738674},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{gisbert_development_2022,

title = {Development, nutrition, and rearing practices of relevant catfish species (Siluriformes) at early stages},

author = {Enric Gisbert and Ronald Kennedy Luz and Ignacio Fernández and Pravata K Pradhan and Maria Salhi and Mansour T Mozanzadeh and Aditya Kumar and Yannis Kotzamanis and Diana Castro‐Ruiz and Martin Bessonart and Maria J Darias},

url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/01/2022-Kotzamanis-RAqua-preprint-2.pdf

https://onlinelibrary.wiley.com/doi/10.1111/raq.12586},

doi = {10.1111/raq.12586},

issn = {1753-5123, 1753-5131},

year  = {2022},

date = {2022-01-01},

urldate = {2022-01-12},

journal = {Reviews in Aquaculture},

volume = {14},

number = {1},

pages = {73--105},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{kotzamanis_dietary_2021,

title = {Dietary lysine requirement of greater amberjack juvenile ( textitSeriola dumerili , Risso, 1810)},

author = {Yannis Kotzamanis and Femi J Fawole and Andreas Brezas and Vikas Kumar and Ramon Fontanillas and Efthimia Antonopoulou and Emmanouil Kouroupakis and Vassiliki Ilia},

url = {https://onlinelibrary.wiley.com/doi/10.1111/anu.13344},

doi = {10.1111/anu.13344},

issn = {1353-5773, 1365-2095},

year  = {2021},

date = {2021-12-01},

urldate = {2022-01-12},

journal = {Aquaculture Nutrition},

volume = {27},

number = {6},

pages = {2107--2118},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{katsika_understanding_2021,

title = {Understanding the Interaction Effects between Dietary Lipid Content and Rearing Temperature on Growth Performance, Feed Utilization, and Fat Deposition of Sea Bass (Dicentrarchus labrax)},

author = {Lydia Katsika and Mario Huesca Flores and Yannis Kotzamanis and Alicia Estevez and Stavros Chatzifotis},

url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/02/2021-Katsika-animals-10.pdf

https://www.mdpi.com/2076-2615/11/2/392},

doi = {10.3390/ani11020392},

year  = {2021},

date = {2021-02-01},

urldate = {2021-02-05},

journal = {Animals},

volume = {11},

number = {2},

pages = {392},

abstract = {This study was conducted to elucidate the interaction effects of temperature and dietary lipid levels (2 × 2 factorial experiment) on the growth performance, muscle, and liver composition in adult farmed European sea bass (Dicentrarchus labrax). Two groups of fish (190 g; 60 fish per group) were distributed in 12 tanks in triplicates and kept at two different temperature regimes; one starting at 23 °C and then changed to 17 °C for 61 days, and the other starting at 17 °C and then changed to 23 °C for 39 days. Two commercial diets containing both textasciitilde44% crude protein but incorporating different dietary lipid levels, 16.5% (D16) and 20.0% (D20) (dry matter (DM)), were fed to the fish to apparent satiation; the type of diet fed to each fish group remained constant throughout the experiment. Final body weight, weight gain, and specific growth rate were significantly higher for the fish group held at 23 °C compared to the fish group at 17 °C (before the temperature changes), while the dietary fat content did not have any profound effect in both groups. Furthermore, the different temperature regimes did not affect muscle or liver composition, but, on the contrary, dietary lipids affected hepatosomatic, perivisceral fat, and visceral indexes. Feed conversion ratio and specific growth rate were not affected by the dietary lipid level. An interaction of temperature and dietary lipid content was observed in daily feed consumption (DFC) and final body weight (FBW).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{ragaza_brown_2021,

title = {Brown seaweed ( textitSargassum fulvellum ) inclusion in diets with fishmeal partially replaced with soy protein concentrate for Japanese flounder ( textitParalichthys olivaceus ) juveniles},

author = {Janice Alano Ragaza and Md. Sakhawat Hossain and Shunsuke Koshio and Manabu Ishikawa and Saichiro Yokoyama and Yannis Kotzamanis and Andreas Brezas and Vikas Kumar},

url = {https://onlinelibrary.wiley.com/doi/10.1111/anu.13246},

doi = {10.1111/anu.13246},

issn = {1353-5773, 1365-2095},

year  = {2021},

date = {2021-01-01},

urldate = {2021-07-26},

journal = {Aquaculture Nutrition},

volume = {27},

number = {4},

pages = {1052--1064},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{kotzamanis_high_2020,

title = {High taurine supplementation in plant protein-based diets improves growth and organoleptic characteristics of European seabass (Dicentrarchus labrax)},

author = {Yannis Kotzamanis and Theofania Tsironi and Andreas Brezas and Kriton Grigorakis and Vassiliki Ilia and Ioannis Vatsos and Nicholas Romano and Jan van Eys and Vikas Kumar},

url = {http://www.nature.com/articles/s41598-020-69014-x},

doi = {10.1038/s41598-020-69014-x},

issn = {2045-2322},

year  = {2020},

date = {2020-12-01},

urldate = {2020-08-10},

journal = {Scientific Reports},

volume = {10},

number = {1},

pages = {12294},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{mastoraki_comparative_2020,

title = {A comparative study on the effect of fish meal substitution with three different insect meals on growth, body composition and metabolism of European sea bass (Dicentrarchus labrax L.)},

author = {Maria Mastoraki and Paula Mollá Ferrándiz and Sofia C Vardali and Demetrius C Kontodimas and Yannis P Kotzamanis and Laura Gasco and Stavros Chatzifotis and Efthimia Antonopoulou},

url = {https://linkinghub.elsevier.com/retrieve/pii/S004484862030065X},

doi = {10.1016/j.aquaculture.2020.735511},

issn = {00448486},

year  = {2020},

date = {2020-11-01},

urldate = {2020-07-06},

journal = {Aquaculture},

volume = {528},

pages = {735511},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{kotzamanis_taurine_2020,

title = {Taurine supplementation in high-soy diets affects fillet quality of European sea bass (Dicentrarchus labrax)},

author = {Yannis Kotzamanis and Vikas Kumar and Theofania Tsironi and Kriton Grigorakis and Vassiliki Ilia and Ioannis Vatsos and Andreas Brezas and Jan [van Eys and Enric Gisbert},

url = {http://www.sciencedirect.com/science/article/pii/S0044848619313900},

doi = {https://doi.org/10.1016/j.aquaculture.2019.734655},

issn = {0044-8486},

year  = {2020},

date = {2020-01-01},

journal = {Aquaculture},

volume = {520},

pages = {734655},

abstract = {This study evaluated the effects of taurine supplementation to diets containing a high dietary inclusion of soybean meal and soy protein concentrate on growth performance and fillet quality of juvenile European sea bass (Dicentrarchus labrax). A control diet (C+) was produced containing high levels of fishmeal (30% FM) and soybean meal (20% SBM). Three other experimental diets were prepared to contain a lower FM inclusion (25%), and a higher amount of soy products (20% of SBM plus 12% soy protein concentrate, SPC) supplemented with three graded levels of crystalline taurine, 0.2%, 0.5% and 1.0% (T0.2, T0.5 and T1.0), respectively. A fifth diet was also prepared having a similar composition as the latter three diets but without the addition of crystalline taurine (negative control diet, C-). All diets were iso-nitrogenous (44%), iso-lipidic (20%) and iso-energetic (22 ​MJ ​kg−1) and were fed to five triplicate groups of sea bass (initial weight 86 g) over the course of a 12-week trial. Dietary taurine supplementation did not affect the growth performance and feed efficiency (P textgreater 0.05). Proximate composition of whole body and muscle were similar among groups (P textgreater 0.05). Taurine dietary supplementation had no effect on the level of intraperitoneal fat deposition (P textgreater 0.05). However, muscle taurine concentration was found to increase gradually in sea bass fed the elevated levels of taurine (P textless 0.05). Interestingly, the hardness and chewiness of the fillet, recorded by texture analysis, increased significantly at higher dietary taurine levels (P textless 0.05). The highest adhesiveness values were obtained in sea bass fed the C- diet, whereas the lowest ones were found in fish fed the T1.0 diet (P textless 0.05). No significant (P textgreater 0.05) impact of diets on texture fillet springiness and cohesiveness was found (P textgreater 0.05). In general, no significant differences were observed by the test panel, however, fish fed the diet supplemented with 1.0% taurine exhibited lower fillet elasticity, thus indicating a potential textural difference in accordance with those obtained from the texture analysis of fish muscles. The histological analysis did not indicate any differences in the gut and liver of the fish fed the experimental diets. Overall, the findings of the present study showed that 1.0% taurine supplementation in diets incorporating high levels of soy products might have a pronounced effect on flesh quality of European sea bass.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{kotzamanis_effects_2018,

title = {Effects of high-level fishmeal replacement by plant proteins supplemented with different levels of lysine on growth performance and incidence of systemic noninfectious granulomatosis in meagre (Argyrosomus regius)},

author = {Y Kotzamanis and E Kouroupakis and V Ilia and J Haralabous and N Papaioannou and K Papanna and R Richards and E Gisbert},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85056334799&doi=10.1111%2fanu.12814&partnerID=40&md5=802b0a99ecb9ce48331ab78ef8d30e87},

doi = {10.1111/anu.12814},

issn = {13535773},

year  = {2018},

date = {2018-01-01},

journal = {Aquaculture Nutrition},

volume = {24},

number = {6},

pages = {1738--1751},

abstract = {The potential use of plant protein (PP) blends (soybean, wheat, rapeseed, corn gluten and wheat gluten) in the diet of juvenile meagre (Argyrosomus regius) was investigated at increasing levels to replace fishmeal (FM) (33% and 56%) in six isonitrogenous (480 g/kg) and isoenergetic (22 MJ/kg) diets, which were supplemented with crystalline lysine. Meagre juveniles (36 ± 0.6 g initial weight) were reared in triplicate for 60 days at 19.4 ± 2.4°C to evaluate their growth performance, feed utilization parameters, body proximate composition and the prevalence of systemic noninfectious granulomatosis. Results indicated that there was no significant difference (GLM ANOVA, p textgreater 0.05) in growth performance and feed utilization parameters in meagre fed the diet containing 300 g/kg FM (33% FM replacement) compared to the control group (450 g/kg FM inclusion), although a trend showing inferior body gain and feed conversion ratio was observed. However, higher levels of FM replacement (56%) by PP blends (200 g/kg FM inclusion) significantly impaired growth performance, feed conversion and protein efficiency rates (p textless 0.05), which may be linked to a decrease in feed intake and/or reduced levels of bioactive compounds or other micronutrients present in FM. On the other hand, increasing dietary lysine levels from 25 to 29 g/kg in the diets containing the same PP content and 200 g/kg inclusion of FM significantly improved growth performance in juvenile meagre. The replacement of FM did not affect lipidosomatic and hepatosomatic indexes in any of the experimental groups evaluated (p textgreater 0.05). The aetiology of granulomatosis found in different tissues was not due to the presence of bacteria, as no bacterial structures were detected in histological slides when samples were stained with the Gram, Ziehl-Neelsen and Fite-Faraco staining. The presence of chronic systemic noninfectious granulomatosis was observed in meagre from all the experimental groups regardless the level of FM replacement by PP blends, indicating that the onset and progression of granulomatosis occurred insidiously at earlier life stages of meagre and persisted at variable levels thereafter. The liver and kidney were found to be the most severely affected tissues. © 2018 John Wiley & Sons Ltd},

note = {Publisher: Blackwell Publishing Ltd},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{vardali_rapid_2018,

title = {Rapid confirmatory method for the determination of danofloxacin and N-desmethyl danofloxacin in european seabass by UPLC-PDA},

author = {S C Vardali and V F Samanidou and Y P Kotzamanis},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041502011&doi=10.2174%2f1573411012666160614081139&partnerID=40&md5=d3ffa1e0f520245fc4549367cd1ef36f},

doi = {10.2174/1573411012666160614081139},

issn = {15734110},

year  = {2018},

date = {2018-01-01},

journal = {Current Analytical Chemistry},

volume = {14},

number = {1},

pages = {68--74},

abstract = {Background: Danofloxacin is a synthetic antibacterial agent with broad spectrum antibacterial and antimycoplasmal activity which is widely used in aquaculture. Methods: A UPLC (Ultra Performance Liquid Chromatography) method was developed and validated for the determination of danofloxacin (Dano) and its major metabolite N-desmethyl danofloxacin (Ndesmethyl Dano) in muscle plus skin tissue of European seabass (Dicentrarchus labrax) for the first time. For the separation an analytical column UPLC BEH C18 2.1 × 5 mm (1.7 µm) was used with an isocratic mobile phase consisting of MeOH-water acidified with formic acid (0.01%) (18:82; v/v) and flow rate of 0.3 mL/min within 5.5 min. The detection was performed at 275 nm using a photodiode array detector for both compounds. Examined fluoroquinolones were isolated from seabass muscle and skin tissue by extraction with acidic ACN and further purification with the QuEChERS methodology. Results: Recovery rates from muscle and skin tissue ranged between 90.2 and 101.2% for both compounds. The detection limit of the method was estimated at 13.73 µg/kg for Dano, 18.32 µg/kg for Ndesmethyl Dano, while the limits of quantification were 41.62 and 55.52 µg/kg, respectively. The developed method was fully validated in terms of selectivity, linearity, accuracy, precision, stability and sensitivity according to the European Union Decision 2002/657/EC. © 2018 Bentham Science Publishers.},

note = {Publisher: Bentham Science Publishers B.V.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{vardali_development_2018,

title = {Development and validation of an ultra performance liquid chromatography-quadrupole time of flight-mass spectrometry (in MSE mode) method for the quantitative determination of 20 antimicrobial residues in edible muscle tissue of European sea bass},

author = {S C Vardali and V F Samanidou and Y P Kotzamanis},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85053198841&doi=10.1016%2fj.chroma.2018.09.017&partnerID=40&md5=edc0011bae4f941c97c541fd3c3410cf},

doi = {10.1016/j.chroma.2018.09.017},

issn = {00219673},

year  = {2018},

date = {2018-01-01},

journal = {Journal of Chromatography A},

volume = {1575},

pages = {40--48},

abstract = {A UPLC-QTOF-MS method for the simultaneous determination of 20 veterinary drug residues and metabolites (tetracyclines, quinolones, sulfonamides and diaminopyrimidines) in edible muscle plus skin tissue of European sea bass (Dicentrarchus Labrax) was developed. For the identification of analytes a positive electrospray ionization quadropole time-of flight mass spectrometer operating in MSE mode (UPLC-QTOF-MSE) was used. MSE mode provides high chromatographic resolution and accurate mass measurements in both MS and MS/MS modes simultaneously in a single run. Separation was achieved on a UPLC BEH C18 (50 mm × 2.1 mm, 1.7 μm) column in a gradient elution program of 10 min. Examined antibiotics were isolated easily after a simple solid-liquid extraction procedure with acidic acetonitrile (0.1% v/v formic acid) and Na2EDTA 0.1 M. Recovery rates from muscle plus skin tissue ranged from 93.8% to 107.5% for all targeted compounds. The detection limits and the limits of quantification ranged from 2.22 to 15.00 μg/kg, and from 6.67 to 45.46 μg/kg, respectively. The developed method was validated in terms of selectivity, matrix effect, linearity, accuracy, precision, stability and sensitivity, CCα and CCβ according to European Union Decision 2002/657/EC. The proposed method was applied for the analysis of contaminated fish samples after in feed administration of danofloxacin mesylate. © 2018 Elsevier B.V.},

note = {Publisher: Elsevier B.V.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{vardali_danofloxacin_2017,

title = {Danofloxacin depletion from muscle plus skin tissue of European sea bass (Dicentrarchus labrax) fed danofloxacin mesylate medicated feed in seawater at 16 °C and 27 °C},

author = {S C Vardali and Y P Kotzamanis and A E Tyrpenou and V F Samanidοu},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021677464&doi=10.1016%2fj.aquaculture.2017.06.036&partnerID=40&md5=0f972570d7da39528df9aeca748124c0},

doi = {10.1016/j.aquaculture.2017.06.036},

issn = {00448486},

year  = {2017},

date = {2017-01-01},

journal = {Aquaculture},

volume = {479},

pages = {538--543},

abstract = {Residue depletion of danofloxacin was investigated in European sea bass after a multiple (5 days) in-feed administration of danofloxacin mesylate (10 mg/kg bw per day) under experimental field conditions at water temperature of 16 °C and 27 °C. Ten fish per sampling point were examined during and after treatment. Samples of muscle plus skin in natural proportions were analyzed for danofloxacin determination by Ultra-Performance Liquid Chromatography equipped with an Ultraviolet Photodiode Array Detector (UPLC-PDA). Danofloxacin concentrations in muscle tissues increased during medication period and then decreased rapidly. The elimination half-lives were estimated to be t1/2 = 16.87 h and 21.13 h at 27 °C and 16 °C, respectively. Withdrawal periods (Wt) were calculated after medication using the WT1.4 program and were found to be at 95% tolerance limit, 7 and 4 days at 16 °C and 27 °C, respectively. © 2017 Elsevier B.V.},

note = {Publisher: Elsevier B.V.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{gisbert_weaning_2016,

title = {Weaning wild flathead grey mullet (Mugil cephalus) fry with diets with different levels of fish meal substitution},

author = {E Gisbert and M T Mozanzadeh and Y Kotzamanis and A Estévez},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84971668055&doi=10.1016%2fj.aquaculture.2016.04.035&partnerID=40&md5=a634fa157033cece66c715fb5fe3a5e4},

doi = {10.1016/j.aquaculture.2016.04.035},

issn = {00448486},

year  = {2016},

date = {2016-01-01},

journal = {Aquaculture},

volume = {462},

pages = {92--100},

abstract = {The culture of flathead grey mullet (Mugil cephalus) is based on wild fry captured during their migration into estuarine environments and consequently, optimizing weaning diets is of special importance for this species at this particular stage of development. Thus, authors have tested a weaning protocol for wild flathead grey mullet fry (202 mg initial body weight) during 60 days (18.1 ± 0.3 °C, salinity, 1.2 ± 0.2‰) using compound diets (36% crude protein, 16% crude fat) with different levels of fish meal (FM) substitution by plant protein (PP) sources (50% and 75% of FM replacement by a blend of corn gluten, wheat gluten, soy bean meal and soy protein concentrate). Fry were progressively weaned onto experimental diets during the first 20 days of the trial (25% Artemia metanauplii replacement each 5 days), whereas compound diets were offered at a feed ratio of 5% of stocked biomass until the end of the trial (day 60). A blend of PP sources (corn gluten, wheat gluten and soy protein concentrate) as the main dietary protein sources in combination with crystalline l-lysine and dl-methionine dietary supplementation were as good as a FM-based diet in terms of growth performance, digestive physiology and fish condition (i.e. proximate composition, oxidative stress status). As the cost-benefit proxy analysis of the tested weaning diets indicated, the inclusion of alternative PP was a satisfactory strategy in terms of feed price reduction, since PP50 and PP75 diets were 15.5 and 23.6% cheaper than the FM diet. Although wild flathead grey mullet fry were satisfactorily weaned onto diets containing 75% FM substitution by PP sources, present results indicated that complete FM replacement in weaning diets for this species might also be feasible. Statement of relevance: In this study, authors have tested a weaning protocol for wild flathead grey mullet (Mugil cephalus) fry based on diets with different levels of fish meal substitution by alternative plant protein sources. This is of special importance due to the importance of the aquaculture of this species in several regions of the word, as well as for the use of wild animals for on-growing purposes. Results showed that diets with 75% of fish meal substitution can be successfully used for weaning and on-growing wild fry without any detrimental effect of fry performance and condition. © 2016 Elsevier B.V.},

note = {Publisher: Elsevier B.V.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{feidantsis_effect_2014,

title = {Effect of taurine-enriched diets on the Hsp expression, MAPK activation and the antioxidant defence of the European sea bass (Dicentrarchus labrax)},

author = {K Feidantsis and E Kaitetzidou and N Mavrogiannis and B Michaelidis and Y Kotzamanis and E Antonopoulou},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84903899185&doi=10.1111%2fanu.12096&partnerID=40&md5=33717104f36c4f17b28f24bd6fd1eb7a},

doi = {10.1111/anu.12096},

issn = {13535773},

year  = {2014},

date = {2014-01-01},

journal = {Aquaculture Nutrition},

volume = {20},

number = {4},

pages = {431--442},

abstract = {This study aimed to identify and characterize the cellular and metabolic response in vital organs of Dicentrarchus labrax, after feeding on soy-based diets enriched with different taurine concentrations (T0.2, T0.5, T1.0 w/w). Molecular responses (expression of Hsp70, Hsp90, phosphorylation of p38 mitogen-activated protein kinase-p38MAPK and extracellular signal-regulated kinases-ERK1/2) were addressed. Metabolic and antioxidant capacities such as activities of citrate synthase (CS), malate and lactate dehydrogenase (MDH, L-LDH), superoxide dismutase (SOD), catalase (CAT) and levels of thiobarbituric acid-reactive substances (TBARS) were assessed. The most intense changes were detected in the liver and in the anterior intestine showing that taurine induces important protective biochemical mechanisms. Specifically, in the liver and the anterior intestine, Hsp and MAPK levels, as well as L-LDH activity levels were up-regulated under the effect of taurine diets. T diet had no major effect on the activity levels of CS, SOD and TBARS, except for MDH in the liver and catalase in the anterior intestine, where activity levels of these enzymes were increased under the effect of T0.2 and T1.0, respectively. These results may facilitate monitoring of adequate health status of farmed fish, exploiting the beneficial T effects and could assist at the formulation of aquaculture feeding strategies. © 2014 John Wiley & Sons Ltd.},

note = {Publisher: Blackwell Publishing Ltd},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{skalli_peptide_2014,

title = {Peptide molecular weight distribution of soluble protein fraction affects growth performance and quality in European sea bass (Dicentrarchus labrax) larvae},

author = {A Skalli and J -L Zambonino-Infante and Y Kotzamanis and R Fabregat and E Gisbert},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84896316449&doi=10.1111%2fanu.12058&partnerID=40&md5=8ebbe6336746885c8515331ce9e2bf14},

doi = {10.1111/anu.12058},

issn = {13535773},

year  = {2014},

date = {2014-01-01},

journal = {Aquaculture Nutrition},

volume = {20},

number = {2},

pages = {118--131},

abstract = {Dicentrarchus labrax were fed from 10 to 40 days posthatching with six microdiets differing in the inclusion level (60 g kg-1 and 120 g kg-1) and type of protein hydrolysate (PH; yeast, YPH; pig blood, PBPH; pig red blood cells, PRBCPH). A microdiet containing 120 g kg-1 fish PH (FPH) was used as a control. PH differed in their amino acid (AA) profile and molecular weight distribution and therefore the tested microdiets too. The estimated content in FAA and di- and tripeptides in the FPH microdiet was 2 g kg-1 and 44 g kg-1, respectively. FAA estimated levels in YPH and PBPH microdiets were 26 g kg-1 and 53 g kg-1, whereas levels of di- and tripeptides were 30 g kg-1 and 60 g kg-1, respectively. The estimated levels of FAA in PRBCPH microdiets were 8 g kg-1 and 17 g kg-1, whereas estimated levels of di- and tripeptides were 11 g kg-1 and 22 g kg-1, respectively. Results revealed that FPH may be replaced by alternative PH from yeast and pig blood products, as fish fed those diets performed, in terms of growth, survival, digestive function and incidence of skeletal deformities, as well as those fed the FPH microdiet. Using YPH, PBPH and PRBCPH, the inclusion level of PH in microdiets might be reduced to a half with respect to current practices using FPH. © 2013 John Wiley & Sons Ltd.},

note = {Publisher: Blackwell Publishing Ltd},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{gisbert_protein_2012,

title = {Protein hydrolysates from yeast and pig blood as alternative raw materials in microdiets for gilthead sea bream (Sparus aurata) larvae},

author = {E Gisbert and A Skalli and I Fernández and Y Kotzamanis and J L Zambonino-Infante and R Fabregat},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84858335976&doi=10.1016%2fj.aquaculture.2012.01.007&partnerID=40&md5=7a565e32abef6da7fdaeeb2a80550040},

doi = {10.1016/j.aquaculture.2012.01.007},

issn = {00448486},

year  = {2012},

date = {2012-01-01},

journal = {Aquaculture},

volume = {338-341},

pages = {96--104},

abstract = {In this study, we have evaluated the incorporation of two types of protein hydrolysates at 9 and 12% levels of inclusion, one from yeast (Saccharomyces cerevisiae, YPH) and another one from pig blood (PBPH), in microdiets for gilthead sea bream (Sparus aurata) larvae, and compared these results to a microdiet containing fish protein hydrolysate and another group only fed with enriched live prey (rotifers and Artemia). The trial consisted in substituting up to 75% (wt/wt) the enriched Artemia with the experimental microdiets from 15 to 40. days post-hatch, whereas larvae were exclusively fed on microdiets from 40 to 55. dph. Protein hydrolysates used in the present study were obtained from different raw materials (yeast, pig blood and fish protein concentrate) and differed in their amino acid (AA) profile and in their molecular weight distribution. YPH and PBPH were mainly composed by free amino acids (FAA) (44%, MW textless 200 Da), di- and tripeptides (50%, 200 textless MW textless 500 Da) and 6% of larger polypeptides (500 textless MW textless 2500 Da); whereas the fish protein hydrolysate (FPH) did only contain a minor quantity of FAA (1.5%) and was mainly composed of di- and tripeptides (36.5%) and larger polypeptides (51.4%, 500 textless MW textless 2500. Da). The contents in FAA and di- and tripeptides in the microdiet containing FPH were 0.2 and 4.4%, respectively. FAA levels in microdiets including YPH and PBPH at 9 and 12% were 4.0 and 5.3%, whereas levels of di- and tripeptides were 4.5 and 6.0%, respectively. Results revealed that FPH in microdiets for marine fish larvae may be replaced by alternative protein hydrolysates obtained from yeast and pig blood, as fish fed with those diets performed, in terms of growth, survival, level of maturation of the enterocytes (activity of cytosolic and brush border enzymes) and incidence of skeletal deformities, as well as those larvae fed with only enriched live preys (rotifers and Artemia). Using YPH and PBPH, the inclusion level of protein hydrolysate in microdiets might be reduced to 9% (3% lesser to actual practices using fish protein hydrolysates) without affecting larval performance. Present results suggested the importance of leucine, valine and phenylalanine in fish larval skeletogenesis and in the appearance of skeletal disorders. © 2012 Elsevier B.V.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{slawski_total_2012,

title = {Total fish meal replacement with rapeseed protein concentrate in diets fed to rainbow trout (Oncorhynchus mykiss Walbaum)},

author = {H Slawski and H Adem and R -P Tressel and K Wysujack and U Koops and Y Kotzamanis and S Wuertz and C Schulz},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84860881834&doi=10.1007%2fs10499-011-9476-2&partnerID=40&md5=1728dc27fd870033083cf9ddb66fefb6},

doi = {10.1007/s10499-011-9476-2},

issn = {09676120},

year  = {2012},

date = {2012-01-01},

journal = {Aquaculture International},

volume = {20},

number = {3},

pages = {443--453},

abstract = {The potential of rapeseed protein concentrate as fish meal alternative in diets for rainbow trout (initial average weight 37.8 ± 1.4 g) was evaluated. Nine experimental tanks of a freshwater flow-through system were stocked with 12 fish each. Triplicate groups of fish received isonitrogenous (47.9 ± 0.5% CP) and isoenergetic (22.4 ± 0.2 kJ g-1) experimental diets with 0, 66 and 100% of fish meal substituted with rapeseed protein concentrate (71.2% CP), thereby providing 0, 29 and 43% of dietary protein. As the amino acid profile of rapeseed protein concentrate was comparable to fish meal, there was no need to supplement experimental diets with synthetic amino acids. At the end of the 84 days of feeding period, fish growth performance, feed intake and feed efficiencies were not compromised, when 100% of fish meal in the control diet was replaced with rapeseed protein concentrate, revealing a SGR of 1.19 or 1.10, a FCR of 1.09 or 1.18 and a feed intake of 78.5 or 74.7 g in fish fed on the control diet or fed the diet devoid of fish meal, respectively. Intestinal morphology did not reveal any histological abnormalities in all dietary groups. Blood parameters including haematocrit and haemoglobin as well as glucose, triglycerides and total protein in the plasma were not different between treatment groups. Thus, the rapeseed protein concentrate tested here has great potential as an alternative to fish meal in rainbow trout diets. © 2011 Springer Science+Business Media B.V.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{estevez_effects_2011,

title = {Effects of different levels of plant proteins on the ongrowing of meagre (Argyrosomus regius) juveniles at low temperatures},

author = {A Estévez and L Treviño and Y Kotzamanis and I Karacostas and L Tort and E Gisbert},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79952517035&doi=10.1111%2fj.1365-2095.2010.00798.x&partnerID=40&md5=f0769fb16f2511d31d08e9e99336b51d},

doi = {10.1111/j.1365-2095.2010.00798.x},

issn = {13535773},

year  = {2011},

date = {2011-01-01},

journal = {Aquaculture Nutrition},

volume = {17},

number = {2},

pages = {e572--e582},

abstract = {Four experimental diets with different inclusion levels of plant proteins and fish protein hydrolysates were compared with a commercial diet for meagre (Argyrosomus regius) ongrowing at optimal and suboptimal water temperature. Results in terms of growth in length and weight, conversion efficiency, dietary feed intake and utilization, body composition (whole fish and liver) as well as enzyme and immunological activities are presented. Fish growth was significantly reduced by the inclusion of plant proteins, although further addition of fish protein hydrolysates improved the results. Daily feed intake was not affected by plant protein inclusion in the diets, although the group fed the highest inclusion level showed lower ingestion than the rest of the groups, probably as a consequence of a reduced dietary palatability. The decrease in water temperature during the second part of the experiment had a negative effect on feed intake and fish growth. Gross visceral morphology of meagre fed the experimental diets was not affected, but muscle weight was significantly reduced. Whole body and liver composition was not affected with plant protein inclusion. However, the inclusion of fish protein hydrolysates resulted in a significant increase in fat content, especially in liver cholesterol and steryl esters, with a parallel reduction in protein. Brush border enzymes were affected by plant protein inclusion as well as serum lysozyme that significantly increased in the fish fed the highest inclusion level. As a conclusion, up to 315gkg-1 plant protein (76.2% of total protein content) can be included in the diet for meagre without affecting growth or feed utilization. Higher inclusion levels can also be used if at least 5% fish protein hydrolysate is also included. © 2010 Blackwell Publishing Ltd.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{slawski_replacement_2011,

title = {Replacement of fish meal with rapeseed protein concentrate in diets fed to turbot (Psetta maxima L.).},

author = {H Slawski and H Adem and R P Tressel and K Wysujack and U Koops and Y Kotzamanis and C Schulz},

year  = {2011},

date = {2011-01-01},

journal = {Züchtungskunde},

volume = {83},

number = {6},

pages = {451--460},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{vatsos_monitoring_2010,

title = {Monitoring stress in fish by applying image analysis to their skin mucous cells},

author = {I N Vatsos and Y Kotzamanis and M Henry and P Angelidis and M N Alexis},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77953160103&partnerID=40&md5=e164b9bb6870e5411efbe171878b3580},

issn = {1121760X},

year  = {2010},

date = {2010-01-01},

journal = {European Journal of Histochemistry},

volume = {54},

number = {2},

pages = {107--111},

abstract = {Several authors have previously demonstrated that the number of the skin mucous cells of fish is affected by many stressors. In the present study, two experiments were conducted in order to examine the effects of two common environmental conditions on the morphology of skin of sea bass and particularly on the number and diameter of skin mucous cells. In the first experiment, two groups of sea bass (mean weight 155.6±10.3 g SD) were maintained in two different concentrations of nitrate, 100 and 700 ppm respectively, for 48 h, while a third group was used as control. In the second experiment, sea bass (initial mean weight 78.9±3.1 g SD) were divided into four groups and each group was maintained in a different level of oxygen for 9 weeks. The oxygen concentration in each group was: 3.6±0.2 ppm, 4.7±0.2 ppm, 6.2±0.2 ppm and 8.2±0.2 ppm. In both experiments the effects of the two environmental factors on the morphology of the fish skin were examined histologically and a software containing a visual basic script macro, allowing quantification of the skin mucous cells, was used to analyze the skin tissue sections. Concerning the overall morphology of the skin and the diameter of the skin mucous cells, no differences were noted in both experiments (P>0.05). It was demonstrated however, that fish maintained in the lowest oxygen level and fish maintained in the highest concentration of nitrate exhibited significantly increased number of mucous cells per skin area (mm 2). There is evidence that the enumeration of the skin mucous cells of fish can be used to monitor stress in fish. © I.N. Vatsos et al., 2010 Licensee PAGEPress, Italy.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{liu_effects_2010,

title = {Effects of fish protein hydrolysate levels on growth performance and biological and physiological parameters in tongue sole (Cynoglossus semilaevis Günther, 1873) post larvae},

author = {X D Liu and M Q Liang and L M Zhang and J Y Wang and Q Chang and Wang J L and Y Kotzamanis},

year  = {2010},

date = {2010-01-01},

journal = {Acta Hydrobiologica Sinica},

volume = {34},

number = {2},

pages = {1--8},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{gisbert_development_2009,

title = {Development of digestive enzymes in common dentex Dentex dentex during early ontogeny},

author = {E Gisbert and G Giménez and I Fernández and Y Kotzamanis and A Estévez},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-58149475982&doi=10.1016%2fj.aquaculture.2008.10.039&partnerID=40&md5=1a1f05030afff114858844b2e4d56ea3},

doi = {10.1016/j.aquaculture.2008.10.039},

issn = {00448486},

year  = {2009},

date = {2009-01-01},

journal = {Aquaculture},

volume = {287},

number = {3-4},

pages = {381--387},

abstract = {The digestive physiology of common dentex was studied by assessing the activity of different pancreatic (trypsin, chymotrypsin, amylase and lipase), intestinal (alkaline phosphatase, aminopeptidase N, maltase and leuncine-alanine peptidase) and gastric (pepsin) enzymes from hatching until the juvenile stage (50 dph at 19 °C). Enzymes involved in the digestion of protein, lipid and carbohydrate were present in common dentex larvae at hatching and before the onset of exogenous feeding. The specific activity of trypsin and chymotrypsin in newly hatched larvae was higher than that of amylase and lipase, indicating the importance of these enzymes in the cleavage of yolk proteins and hatching, while after hatching, the activity of these serine proteases dramatically decreased, whereas lipase showed the opposite trend. A progressive shift in activity from alkaline to acid proteases was observed during larval development, reflecting that alkaline proteases were not longer the main digestive enzymes involved in protein digestion after the development of gastric glands and onset of acidic digestion. Lipase total activity in common dentex peaked at 35 dph and decreased after weaning. Diet change due to weaning might partially explain the decrease in lipase activity, although this change might be also indicative of a change in the nutritional requirements of this species, since the juveniles prefer diets with high protein levels than those with lower protein and higher lipid content. Regarding intestinal enzymes, leucine-alanine peptidase and alkaline phosphatase and aminopeptidase N were found in newly hatched larvae, while maltase was detected after the onset of exogenous feeding. The achievement of an efficient brush border membrane digestion takes place much earlier (6-12 dph) than in any of the studied species which might reflect the different metabolic profile and rapid growth of this species. © 2008 Elsevier B.V. All rights reserved.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{fountoulaki_fish_2009,

title = {Fish oil substitution by vegetable oils in commercial diets for gilthead sea bream (Sparus aurata L.); effects on growth performance, flesh quality and fillet fatty acid profile. Recovery of fatty acid profiles by a fish oil finishing diet under fluctuating water temperatures},

author = {E Fountoulaki and A Vasilaki and R Hurtado and K Grigorakis and I Karacostas and I Nengas and G Rigos and Y Kotzamanis and B Venou and M N Alexis},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-61649088364&doi=10.1016%2fj.aquaculture.2009.01.023&partnerID=40&md5=e3b4d18877877d60dcd12caa1fda3f45},

doi = {10.1016/j.aquaculture.2009.01.023},

issn = {00448486},

year  = {2009},

date = {2009-01-01},

journal = {Aquaculture},

volume = {289},

number = {3-4},

pages = {317--326},

abstract = {The effects of long term feeding (6 months) of commercial diets with low fish meal content and high levels of vegetable oils (69% fish oil substitution level) were determined in gilthead sea bream (110 g). A control diet containing South American fish oil (FO) was evaluated against feeds with either soybean oil (SO), palm oil (PO) or rapeseed oil (RO). Afterwards, all fish were fed a fish oil finishing diet to determine the progressive recovery of the fillet fatty acid profiles. The results showed that growth and feed utilization in gilthead sea bream are not affected by fish oil substitution with soybean and rapeseed oil, contrary to palm oil inclusion. Flesh and liver docosahexaenoic acid (DHA) and arachidonic acid (ArA) contents were reduced to a lower degree than their reduction in the diet, whereas eicosapentaenoic (EPA) reduction was more pronounced in both tissues. Sensory analysis revealed no difference in the organoleptic characteristics of the dietary groups. However, low acceptance scores were calculated for all treated groups. No histological alterations were seen in gut tissue but liver of the PO group showed intense lipid accumulation. Re-feeding with a fish oil finishing diet for 120 days was not adequate for restoration of DHA, ArA and EPA. Linoleic (LA) and oleic acid (OA) were retained even after 120 days re-feeding with the fish oil diet. © 2009 Elsevier B.V. All rights reserved.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{adamidou_growth_2009,

title = {Growth, feed utilization, health and organoleptic characteristics of European seabass (Dicentrarchus labrax) fed extruded diets including low and high levels of three different legumes},

author = {S Adamidou and I Nengas and M Henry and K Grigorakis and G Rigos and D Nikolopoulou and Y Kotzamanis and G J Bell and K Jauncey},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-67649088041&doi=10.1016%2fj.aquaculture.2009.04.045&partnerID=40&md5=dfaf34a8796e08dbe50d7099ac4f3f40},

doi = {10.1016/j.aquaculture.2009.04.045},

issn = {00448486},

year  = {2009},

date = {2009-01-01},

journal = {Aquaculture},

volume = {293},

number = {3-4},

pages = {263--271},

abstract = {Three legumes [field peas (P), chickpeas (CP) and faba beans (B)] were evaluated at two inclusion levels; 170 (L) and 350 g kg- 1 (H) in a 14-week experiment with triplicate groups of 97.9 ± 6.1 g European seabass. A control diet included wheat meal, fish meal (FM) and a mixture of plant ingredients as protein sources. Diets, processed in a twin-screw extruder, were isonitrogenous and isoenergetic. All experimental diets improved growth compared to the control. Weight gain, feed intake, FCR and SGR were improved for fish fed diet CPL, while fish fed diet CPH gave higher FCR. Protein and starch digestibility were highest for the control diet, while fish fed diets CPL and PL showed significantly lower protein ADCs and lower starch ADC (P ≤ 0.05) for diet PH. Among H diets, significantly better fillet yield was found for fish fed diet PH, but no other differences were found in seabass fillet organoleptic characteristics. Serum glucose and cholesterol were elevated in fish fed CP diets, while protein and triacylglycerols did not show significant differences among treatments. Fish fed the test diets showed no significant evidence of either immunosuppression or immunostimulation. Histology of liver, spleen, kidney and foregut revealed no pathological abnormalities. Field peas, chickpeas and faba beans can be included in European seabass diets up to 350 g kg- 1 substituting for wheat with no negative effects on growth performance, carcass composition or organoleptic characteristics. © 2009 Elsevier B.V. All rights reserved.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{vatsos_short-term_2009,

title = {Short-term of European sea bass (Dicentrarchus labrax) to nitrate. Effects on skin morphology and skin mucous cells.},

author = {I Vatsos and Y Kotzamanis and D Vectesi and Morgane Henry and P Angelidis},

year  = {2009},

date = {2009-01-01},

journal = {Sc. Annals of DDI},

volume = {15},

pages = {105--108},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{fernandez_larval_2008,

title = {Larval performance and skeletal deformities in farmed gilthead sea bream (Sparus aurata) fed with graded levels of Vitamin A enriched rotifers (Brachionus plicatilis)},

author = {Ignacio Fernández and Francisco Hontoria and Juan B Ortiz-Delgado and Yannis Kotzamanis and Alicia Estévez and Jose Luis Zambonino-Infante and Enric Gisbert},

url = {https://linkinghub.elsevier.com/retrieve/pii/S004484860800464X},

doi = {10.1016/j.aquaculture.2008.06.037},

issn = {00448486},

year  = {2008},

date = {2008-10-01},

urldate = {2020-08-18},

journal = {Aquaculture},

volume = {283},

number = {1-4},

pages = {102--115},

abstract = {Several nutritional studies have found a direct effect of several vitamins in chondrogenic and osteogenic development during early life stages of marine fish species. In the present study, the effect of vitamin A (VA) in gilthead sea bream skeletogenesis was evaluated by means of four different dietary regimes (enriched rotifers) containing increasing levels of total VA (75, 109, 188 and 723 ng total VA mg- 1 DW). Dietary treatments were offered to larvae during the rotifer-feeding phase (4-20 days after hatching), while later all groups were fed with Artemia nauplii and weaned onto the same inert diet. Different dietary doses of VA affected gilthead sea bream larval growth, survival, performance (maturation of the digestive system) and quality (incidence of skeletal deformities). Higher levels of dietary VA than those included in the commercial emulsion for rotifer enrichment led to different levels and typologies of skeletal deformities, indicating that gilthead sea bream larvae were very sensitive to small increases in dietary VA. The degree of ossification was affected by the level of VA in enriched rotifers: the higher amount of VA in the diet, the higher number of skeletal pieces ossified (R = 0.585},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{gimenez_modelling_2007,

title = {Modelling retinoid content in live prey: A tool for evaluating the nutritional requirements and development studies in fish larvae},

author = {G Giménez and Y Kotzamanis and F Hontoria and A Estévez and E Gisbert},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-34250312482&doi=10.1016%2fj.aquaculture.2007.01.022&partnerID=40&md5=76ef723267befaeab5239bc93646bd7e},

doi = {10.1016/j.aquaculture.2007.01.022},

issn = {00448486},

year  = {2007},

date = {2007-01-01},

journal = {Aquaculture},

volume = {267},

number = {1-4},

pages = {76--82},

abstract = {This study was conducted to evaluate the accumulation of different levels of total vitamin A in live prey (Brachionus plicatilis and Artemia nauplii) enriched with a commercial emulsion (0.15 and 0.6 g l- 1 for rotifers and Artemia nauplii, respectively), which contained increasing levels of all-trans retinyl acetate. Emulsions used for rotifer enrichment contained 124, 138, 151, 165, 178, 192, 226, 259 and 327 μg total vitamin A l- 1, whereas those used for Artemia nauplii contained 494, 548, 602, 629, 710, 764, 899, 1034 and 1334 μg total vitamin A l- 1. Total vitamin A incorporation in rotifers was constant until a threshold comprised between 226 and 327 μg total vitamin A l- 1, above which the incorporation of total vitamin A from the emulsion was maximum (806 ng total vitamin A mg DW- 1 in rotifers enriched with 327 μg total vitamin A l- 1). In Artemia nauplii, total vitamin A increased from 4.0 ng mg DW- 1 up to 52 ng mg DW- 1 in nauplii enriched with an emulsion containing 1334 μg total vitamin A l- 1. Retinoid levels in live prey increased as the content of all-trans retinyl acetate augmented in the emulsion, although they did not accumulate in a dose-dependent manner because retinoid incorporation in live prey was found to be not proportional to the content in the emulsion. Rotifers exhibited a higher retinoid incorporation pattern than Artemia nauplii, which seemed to be related to species-specific differences between both live prey. Both live prey were able to absorb and metabolize the vitamin A compounds administered through the emulsion, according to the results regarding retinol and retinoic acid content although the levels were higher in the rotifers than in the nauplii. The differential pattern of total vitamin A accumulation between rotifers and Artemia nauplii should be considered when designing nutritional studies dealing with this vitamin and first feeding marine larvae reared on live prey due to the difficulty in maintaining constant levels of total vitamin A especially during the transition feeding phase from rotifers to Artemia nauplii. © 2007 Elsevier B.V. All rights reserved.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{kotzamanis_effects_2007,

title = {Effects of different dietary levels of fish protein hydrolysates on growth, digestive enzymes, gut microbiota, and resistance to Vibrio anguillarum in European sea bass (Dicentrarchus labrax) larvae},

author = {Y P Kotzamanis and E Gisbert and F J Gatesoupe and J Zambonino Infante and C Cahu},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33947526750&doi=10.1016%2fj.cbpa.2006.12.037&partnerID=40&md5=ea0a2c3ce2b5e68edd11c12ea217eff8},

doi = {10.1016/j.cbpa.2006.12.037},

issn = {10956433},

year  = {2007},

date = {2007-01-01},

journal = {Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology},

volume = {147},

number = {1},

pages = {205--214},

abstract = {Two fish protein hydrolysates (FPH) were incorporated into four diets prepared for start-feeding sea bass larvae, at two different levels (10% and 19% of total ingredients): a commercial FPH, CPSP, in which the molecular mass of the main fraction of soluble peptides (51%) was between 500-2500 Da, and an experimental FPH obtained by acidic silage of sardine offal, SH, with a main portion of soluble peptides (54%) ranging from 200 to 500 Da. The diet with 10% of the commercial FPH gave the best results in terms of growth, survival and intestinal development, as evaluated by the early activity of digestive enzymes in the brush border membrane (alkaline phosphatase and aminopeptidase N). This was related to the low level of Vibrio spp. counted in the larvae of group C10. The high dose of FPH, especially in the experimental preparation rich in short peptides, seemed to favour the dominance of Vibrio sp. TYH3, which behaved opportunistically. The effect of the experimental FPH was ambiguous, since early larvae challenged with Vibrio anguillarum were more resistant to the pathogen, especially at high FPH dose (group S19). This might be due either to direct antagonism between V. anguillarum and Vibrio sp. TYH3, or to the stimulation of the immune response in the larvae. These results indicate that different molecular weight fractions and concentrations of feed-soluble peptides may affect the growth performance and immunological status of sea bass larvae. Consequently, a low dose of commercial FPH seems advisable, both for larval development and for the bacterial environment, although further research is required to determine and characterize peptide fractions that may have a beneficial effect on growth and immune response, and to determine their optimal inclusion levels in diets for sea bass larvae. © 2007 Elsevier Inc. All rights reserved.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{moraiti-ioannidou_fatty_2007,

title = {Fatty acid composition and biometry of five Greek Artemia populations suitable for aquaculture purposes},

author = {M Moraiti-Ioannidou and J Castritsi-Catharios and H Miliou and Y P Kotzamanis},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-35948966813&doi=10.1111%2fj.1365-2109.2007.01835.x&partnerID=40&md5=278bd71b0d3bf8726f818dbfa97ca58e},

doi = {10.1111/j.1365-2109.2007.01835.x},

issn = {1355557X},

year  = {2007},

date = {2007-01-01},

journal = {Aquaculture Research},

volume = {38},

number = {15},

pages = {1664--1672},

abstract = {Evaluation of a new-to-science Artemia population and comparison with four other existing populations were carried out. Five parthenogenetic Artemia populations from the following Greek saltworks were studied: Alyki (a new population), Kalloni, Milos, Polychnitos and Messolongi. The diameters of non-decapsulated and decapsulated cysts as well as the lengths of nauplii instar stages I, II and III were measured. In addition, the fatty acid composition of decapsulated cysts was estimated. The cyst size of the Alyki population was the smallest, with the largest chorion found in Greece so far. The Messolongi population had the largest cysts, with the smallest chorion of all five populations. The diameter of the non-decapsulated cysts was highly related (Ptextless0.01) to the length of the nauplii instar I, II and III. The diameter of decapsulated cysts was found to be the most stable and reliable biometric characteristic. The study of their fatty acids profiles revealed that the Polychnitos and Messolongi populations are suitable as live food for freshwater species, while the Alyki, Milos and Kalloni populations are suitable for marine species, having high levels (8.9%, 11.2% and 12.5% respectively) of eicosapentaenoic acid and detectable amounts (0.3%, 0.1% and 0.2% respectively) of docosahexaenoic acid. © 2007 The Authors.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{xylouri_isolation_2007,

title = {Isolation, characterization, and sequencing of nodavirus in sturgeon (Acipenser gueldestaedi L.) reared in freshwater facilities},

author = {E Xylouri and Y P Kotzamanis and F Athanasso Poulou and L Dong and I S Pappas and A Argyrokastritis and E Fragkiadaki},

url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-34047204411&partnerID=40&md5=613617628f6d5e8ad3ea10829146183e},

year  = {2007},

date = {2007-01-01},

journal = {Israeli Journal of Aquaculture - Bamidgeh},

volume = {59},

pages = {36 -- 41},

abstract = {The study demonstrates the presence of a nodavirus that affected sturgeon in fresh water, causing disease with neurological signs. The virus was isolated and inoculated onto SSN-1 (striped snakehead, Channa striatus) cell cultures where cytopathic effects (CPE) of the virus included vacuolation of the cells and degeneration of the monolayer. A 255 bp amplicon from nucleic acid preparations of brain tissue from infected sturgeon was detected by PCR (RT-PCR and nested-PCR) and compared with corresponding amino acid sequences of other infected species. The sequences from the sturgeon were similar to those of sea bass, red spotted grouper, and European eel, supporting the hypothesis that the virus originated in marine fish and was horizontally transmitted to freshwater sturgeon.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{tyrpenou_flumequine_2003,

title = {Flumequine depletion from muscle plus skin tissue of gilthead seabream (Sparus aurata L.) fed flumequine medicated feed in seawater at 18 and 24 °C},

author = {A E Tyrpenou and Y P Kotzamanis and M N Alexis},

url = {https://linkinghub.elsevier.com/retrieve/pii/S004484860200251X},

doi = {10.1016/S0044-8486(02)00251-X},

issn = {00448486},

year  = {2003},

date = {2003-04-01},

urldate = {2020-08-18},

journal = {Aquaculture},

volume = {220},

number = {1-4},

pages = {633--642},

abstract = {We examined flumequine depletion from muscle plus skin of gilthead seabream held in seawater at 18 and 24°C. Seven groups of 10 fish each were sampled at intervals ranging from 24 to 168 h after in-feed administration of flumequine at 35 mg/kg/day for 5 days. Muscle plus skin tissue samples were analyzed for flumequine by high-performance liquid chromatography and fluorescence detection (HPLC-SFD). Parent flumequine concentrations declined rapidly from muscle plus skin after dosing with elimination half-lives of t1/2=22.14 and 21.43 h at 18 and 24°C, respectively. Withdrawal periods for the maximum residue limit (MRL) of 600 μg/kg flumequine in muscle plus skin at 95% tolerance limit were 106.08 and 75.84 h at 18 and 24°C, respectively, after treatment. © 2003 Elsevier Science B.V. All rights reserved.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{kotzamanis_utilization_2001,

title = {Utilization of waste material resulting from trout processing in gilthead bream (Sparus aurata L.) diets},

author = {Y P Kotzamanis and M N Alexis and A Andriopoulou and I Castritsi-Cathariou and G Fotis},

url = {http://doi.wiley.com/10.1046/j.1355-557x.2001.00042.x},

doi = {10.1046/j.1355-557x.2001.00042.x},

issn = {1355557X, 13652109},

year  = {2001},

date = {2001-12-01},

urldate = {2020-08-18},

journal = {Aquaculture Research},

volume = {32},

pages = {288--295},

abstract = {Fish processing creates a large amount of waste of high nutrient content which, if not properly processed for use in human or animal nutrition, is likely to be deposited in the environment creating pollution problems. Waste parts from rainbow trout processing for smoking, consisting of heads, bones, tails and intestines, were used as feed ingredients for gilthead bream diets. Heads, bones and tails had similar compositions, their weighed mean indicating about 700 g kg-1 moisture, 150 g kg-1 protein and 110 g kg-1 fat. Intestines contained higher lipid (350 g kg-1) and lower moisture (560 g kg-1) and protein content (80 g kg-1). Seasonal changes in composition indicated significant differences. Three experimental diets were formulated having the same proximate composition on a dry weight basis. The control diet (A) contained fish meal as the main protein source and fish oil as the oil supplement. In diet B part of the protein and most of lipid was provided by trout waste and in diet C most of the lipid was provided by trout intestines. Gilthead bream fingerlings of 4 g initial weight were fed to apparent satiation for 72 days, at a temperature of 20°C, to an average final weight of 19 g. All diets were fed in a dry form. The experiment was performed in duplicate. Growth and feed utilization data were high and similar among groups. The body composition of the resulting fish did not show any difference among dietary treatments. Differences in liver lipid and fatty acid content were found between all dietary treatments. The growth and body composition data from this preliminary experiment indicated that trout waste could be used successfully as a dietary ingredient of sea bream diets. © 2001 Blackwell Science Ltd.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}