2023 |
Triga, Adriana; Smyrli, Maria; Katharios, Pantelis Microorganisms, 11 (5), pp. 1197, 2023, ISSN: 2076-2607. @article{triga_pathogenic_2023, title = {Pathogenic and Opportunistic Vibrio spp. Associated with Vibriosis Incidences in the Greek Aquaculture: The Role of Vibrio harveyi as the Principal Cause of Vibriosis}, author = {Adriana Triga and Maria Smyrli and Pantelis Katharios}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/05/2023-Triga-microorganisms-19.pdf https://www.mdpi.com/2076-2607/11/5/1197}, doi = {10.3390/microorganisms11051197}, issn = {2076-2607}, year = {2023}, date = {2023-05-17}, urldate = {2023-05-17}, journal = {Microorganisms}, volume = {11}, number = {5}, pages = {1197}, abstract = {A monitoring program to follow vibriosis incidents in the Greek marine aquaculture was implemented over the past 13 years. 273 isolates, from various cases originating from eight regions and nine hosts, were collected and characterized. The main aquaculture species of the survey were the European seabass (Dicentrarchus labrax) and the gilthead seabream (Sparus aurata). Various species of Vibrionaceae were associated with vibriosis. Vibrio harveyi had the highest prevalence and was isolated throughout the year from all hosts. During the warm months, Vibrio harveyi prevailed with frequent co-isolations of Photobacterium damselae subsp. damselae and Vibrio alginolyticus, while during spring, other Vibrio species were more abundant, such as Vibrio lentus, Vibrio cyclitrophicus, and Vibrio gigantis. Phylogenetic analysis using the mreB gene and the metabolic fingerprint of the isolates showed great variability within the species of the collection. The severity of the disease and the frequency of outbreaks make vibriosis (that is, mainly attributed to V. harveyi) an important concern for the regional aquaculture sector.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A monitoring program to follow vibriosis incidents in the Greek marine aquaculture was implemented over the past 13 years. 273 isolates, from various cases originating from eight regions and nine hosts, were collected and characterized. The main aquaculture species of the survey were the European seabass (Dicentrarchus labrax) and the gilthead seabream (Sparus aurata). Various species of Vibrionaceae were associated with vibriosis. Vibrio harveyi had the highest prevalence and was isolated throughout the year from all hosts. During the warm months, Vibrio harveyi prevailed with frequent co-isolations of Photobacterium damselae subsp. damselae and Vibrio alginolyticus, while during spring, other Vibrio species were more abundant, such as Vibrio lentus, Vibrio cyclitrophicus, and Vibrio gigantis. Phylogenetic analysis using the mreB gene and the metabolic fingerprint of the isolates showed great variability within the species of the collection. The severity of the disease and the frequency of outbreaks make vibriosis (that is, mainly attributed to V. harveyi) an important concern for the regional aquaculture sector. |
Tsertou, Maria Ioanna; Triga, Adriana; Droubogiannis, Stavros; Kokkari, Constantina; Anasi, Grammatiki; Katharios, Pantelis Frontiers in Microbiology, 14 , pp. 1078669, 2023, ISSN: 1664-302X. @article{tsertou_isolation_2023, title = {Isolation and characterization of a novel Tenacibaculum species and a corresponding bacteriophage from a Mediterranean fish hatchery: Description of Tenacibaculum larymnensis sp. nov. and Tenacibaculum phage Larrie}, author = {Maria Ioanna Tsertou and Adriana Triga and Stavros Droubogiannis and Constantina Kokkari and Grammatiki Anasi and Pantelis Katharios}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/03/2023-Tsertou-FroMicro-13.pdf https://www.frontiersin.org/articles/10.3389/fmicb.2023.1078669/full}, doi = {10.3389/fmicb.2023.1078669}, issn = {1664-302X}, year = {2023}, date = {2023-03-07}, urldate = {2023-03-07}, journal = {Frontiers in Microbiology}, volume = {14}, pages = {1078669}, abstract = {Tenacibaculum larymnensis sp. nov., a novel species of the Tenacibaculum genus was isolated from a commercial fish hatchery in Greece. The novel species is phylogenetically close to T. discolor and was biochemically and genetically characterized. The genome of T . larymnensis has 3.66 Mbps length, 31.83% GC content and the genomic analysis demonstrated that it harbors a wide enzymatic repertoire suggestive of increased degrading capacity but also several virulence factors including hemolysins, secretion systems, transporters, siderophores, pili and extracellular proteins. Using the novel strain, a virulent bacteriophage designated as Tenacibaculum phage Larrie was isolated and characterized. Larrie is a novel Siphovirus with relatively large genome, 77.5 kbps with 111 ORFs, a GC content of 33.7% and an exclusively lytic lifestyle. The new phage-host system can serve as an efficient model to study microbial interactions in the aquatic environment which contribute to the nutrient cycling.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Tenacibaculum larymnensis sp. nov., a novel species of the Tenacibaculum genus was isolated from a commercial fish hatchery in Greece. The novel species is phylogenetically close to T. discolor and was biochemically and genetically characterized. The genome of T . larymnensis has 3.66 Mbps length, 31.83% GC content and the genomic analysis demonstrated that it harbors a wide enzymatic repertoire suggestive of increased degrading capacity but also several virulence factors including hemolysins, secretion systems, transporters, siderophores, pili and extracellular proteins. Using the novel strain, a virulent bacteriophage designated as Tenacibaculum phage Larrie was isolated and characterized. Larrie is a novel Siphovirus with relatively large genome, 77.5 kbps with 111 ORFs, a GC content of 33.7% and an exclusively lytic lifestyle. The new phage-host system can serve as an efficient model to study microbial interactions in the aquatic environment which contribute to the nutrient cycling. |
2022 |
Triga, Adriana; Smyrli, Maria; Katharios, Pantelis Aeromoniasis Incollection Aquaculture Pathophysiology, pp. 317–327, Elsevier, 2022, ISBN: 9780128122112. @incollection{triga_aeromoniasis_2022, title = {Aeromoniasis}, author = {Adriana Triga and Maria Smyrli and Pantelis Katharios}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/10/2022-Tringa-chapter-25-Aeromonas-preprint-68.pdf https://linkinghub.elsevier.com/retrieve/pii/B978012812211200024X}, doi = {10.1016/B978-0-12-812211-2.00024-X}, isbn = {9780128122112}, year = {2022}, date = {2022-01-01}, urldate = {2022-10-12}, booktitle = {Aquaculture Pathophysiology}, pages = {317--327}, publisher = {Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {incollection} } |
2021 |
Kogiannou, Dimitra; Nikoloudaki, Chrysanthi; Katharios, Pantelis; Triga, Adriana; Rigos, George Evaluation of absorption and depletion of florfenicol in European seabass textitDicentrarchus labrax Journal Article Veterinary Medicine and Science, 7 (3), pp. 987–997, 2021, ISSN: 2053-1095, 2053-1095. @article{kogiannou_evaluation_2021, title = {Evaluation of absorption and depletion of florfenicol in European seabass textitDicentrarchus labrax}, author = {Dimitra Kogiannou and Chrysanthi Nikoloudaki and Pantelis Katharios and Adriana Triga and George Rigos}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/06/2021-Kogiannou-VetMed-Sci-40.pdf https://onlinelibrary.wiley.com/doi/10.1002/vms3.415}, doi = {10.1002/vms3.415}, issn = {2053-1095, 2053-1095}, year = {2021}, date = {2021-05-01}, urldate = {2021-06-24}, journal = {Veterinary Medicine and Science}, volume = {7}, number = {3}, pages = {987--997}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2019 |
Smyrli, Maria; Triga, Adriana; Dourala, Nancy; Varvarigos, Panos; Pavlidis, Michael; Quoc, Viet Ha; Katharios, Pantelis Comparative study on a novel pathogen of european seabass. Diversity of Aeromonas veronii in the aegean sea Journal Article Microorganisms, 7 (11), 2019, ISSN: 20762607. @article{smyrli_comparative_2019, title = {Comparative study on a novel pathogen of european seabass. Diversity of Aeromonas veronii in the aegean sea}, author = {Maria Smyrli and Adriana Triga and Nancy Dourala and Panos Varvarigos and Michael Pavlidis and Viet Ha Quoc and Pantelis Katharios}, doi = {10.3390/microorganisms7110504}, issn = {20762607}, year = {2019}, date = {2019-01-01}, journal = {Microorganisms}, volume = {7}, number = {11}, abstract = {Aeromonas veronii is an emerging pathogen causing severe pathology and mortalities in European seabass aquaculture in the Aegean Sea, Mediterranean. More than 50 strains of the pathogen were characterized biochemically and genetically in order to study the epidemiology of the disease, as well as the phylogeny and virulence of the bacterium. Based on the phenotypic characteristics, the isolates form three groups consisting of: (a) the West Aegean Sea, non-motile, non-pigment-producing strains, (b) the West Aegean Sea, motile, and pigment-producing strains and (c) the East Aegean Sea motile strains that produce minute amounts of pigment. All strains were highly similar at the genomic level; however, the pattern of West/East geographic origin was reflected in biochemical properties, in general genomic level comparison and in the putative virulent factors studied. Type VI secretion system was not detected in the western strains. The outer membrane protein (OMP) profile which contains proteins that are putative antigenic factors, was very similar between strains from the different areas. Although most of the OMPs were detected in all strains with great sequence similarity, diversification according to geographic origin was evident in known antigenic factors such as the maltoporin LamB. A systematic comparative analysis of the strains is presented and discussed in view of the emergence of A. veronii as a significant pathogen for the Mediterranean aquaculture.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Aeromonas veronii is an emerging pathogen causing severe pathology and mortalities in European seabass aquaculture in the Aegean Sea, Mediterranean. More than 50 strains of the pathogen were characterized biochemically and genetically in order to study the epidemiology of the disease, as well as the phylogeny and virulence of the bacterium. Based on the phenotypic characteristics, the isolates form three groups consisting of: (a) the West Aegean Sea, non-motile, non-pigment-producing strains, (b) the West Aegean Sea, motile, and pigment-producing strains and (c) the East Aegean Sea motile strains that produce minute amounts of pigment. All strains were highly similar at the genomic level; however, the pattern of West/East geographic origin was reflected in biochemical properties, in general genomic level comparison and in the putative virulent factors studied. Type VI secretion system was not detected in the western strains. The outer membrane protein (OMP) profile which contains proteins that are putative antigenic factors, was very similar between strains from the different areas. Although most of the OMPs were detected in all strains with great sequence similarity, diversification according to geographic origin was evident in known antigenic factors such as the maltoporin LamB. A systematic comparative analysis of the strains is presented and discussed in view of the emergence of A. veronii as a significant pathogen for the Mediterranean aquaculture. |
Adriana Triga
2023 |
Microorganisms, 11 (5), pp. 1197, 2023, ISSN: 2076-2607. |
Frontiers in Microbiology, 14 , pp. 1078669, 2023, ISSN: 1664-302X. |
2022 |
Aeromoniasis Incollection Aquaculture Pathophysiology, pp. 317–327, Elsevier, 2022, ISBN: 9780128122112. |
2021 |
Evaluation of absorption and depletion of florfenicol in European seabass textitDicentrarchus labrax Journal Article Veterinary Medicine and Science, 7 (3), pp. 987–997, 2021, ISSN: 2053-1095, 2053-1095. |
2019 |
Comparative study on a novel pathogen of european seabass. Diversity of Aeromonas veronii in the aegean sea Journal Article Microorganisms, 7 (11), 2019, ISSN: 20762607. |