Publications – IMBBC

HCMR Authors:

Results for Author Fakriadis Ioannis :

2025

Lancerotto, Stefano; Fakriadis, Ioannis; Papadaki, Maria; Sigelaki, Irini; Giménez, Ignacio; Genoves, Jose Vicente Roig; Meloni, Andrea; Mylonas, Constantinos C

Administration of single-chain recombinant follicle-stimulating hormone (sdrFsh) and luteinizing hormone (sdrLh) stimulates spermatogenesis, but not vitellogenesis in pre-pubertal greater amberjack (Seriola dumerili) Journal Article

Aquaculture, 609 , pp. 742795, 2025, ISSN: 00448486.

Links | BibTeX

Lancerotto, Stefano; Fakriadis, Ioannis; Papadaki, Maria; Giménez, Ignacio; Genovés, José Vicente Roig; Mylonas, Constantinos C

Overcoming dysfunctional gametogenesis in hatchery-produced greater amberjack, Seriola dumerili using recombinant gonadotropins, and spawning induction using gonadotropin releasing hormone agonist-loaded implants Journal Article

Aquaculture, 594 , pp. 741401, 2025, ISSN: 00448486.

Links | BibTeX

2024

Superio, Joshua; Resseguier, Julien; Nobrega, Rafael Henrique; Grebstad, Caroline M; Fakriadis, Ioannis; Foss, Atle; Hagen, Ørjan; Zhang, Meiling; García-Hernández, Maria Del Pilar; Galindo-Villegas, Jorge

Unravelling spermatogenesis in spotted wolffish: Insights from the ultrastructure of juvenile male testes to the cryopreservation of broodstock sperm Journal Article

Aquaculture, 592 , pp. 741214, 2024, ISSN: 00448486.

Links | BibTeX

Papadaki, Maria; Karamanlidis, Dimitris; Sigelaki, Eirini; Fakriadis, Ioannis; Mylonas, Constantinos C

Evolution of sex ratio and egg production of gilthead seabream (Sparus aurata) over the course of five reproductive seasons Journal Article

Aquaculture and Fisheries, 9 (4), pp. 534–542, 2024, ISSN: 2468550X.

Links | BibTeX

Katirtzoglou, Aikaterini; Tsaparis, Dimitris; Kolios, Evangelos; Magoulas, Antonios; Mylonas, Constantinos C; Fakriadis, Ioannis; Manousaki, Tereza; Tsigenopoulos, Costas S

Population genomic analysis of the greater amberjack (Seriola dumerili) in the Mediterranean and the Northeast Atlantic, based on SNPs, microsatellites, and mitochondrial DNA sequences Journal Article

Frontiers in Fish Science, 2 , pp. 1356313, 2024, ISSN: 2813-9097.

Abstract | Links | BibTeX

@article{katirtzoglou_population_2024,
title = {Population genomic analysis of the greater amberjack (\textit{Seriola dumerili}) in the Mediterranean and the Northeast Atlantic, based on SNPs, microsatellites, and mitochondrial DNA sequences},
author = {Aikaterini Katirtzoglou and Dimitris Tsaparis and Evangelos Kolios and Antonios Magoulas and Constantinos C Mylonas and Ioannis Fakriadis and Tereza Manousaki and Costas S Tsigenopoulos},
url = {https://imbbc.hcmr.gr/wp-content/uploads/2024/03/2024-Kaitirtzoglou-frish-14.pdf
https://www.frontiersin.org/articles/10.3389/frish.2024.1356313/full},
doi = {10.3389/frish.2024.1356313},
issn = {2813-9097},
year = {2024},
date = {2024-03-14},
urldate = {2024-03-14},
journal = {Frontiers in Fish Science},
volume = {2},
pages = {1356313},
abstract = {The greater amberjack (
Seriola dumerili
) has attracted considerable economic interest since the 1990s because it possesses traits that distinguish it as a promising candidate in aquaculture. However, the challenges of its successful rearing, combined with the need to develop proper management practices, underline the importance of research into the genetic diversity of both wild and farmed stocks. We aimed to decipher the genetic structure of
S. dumerili
in the Eastern Mediterranean and the Northeast Atlantic using three types of genetic markers (mitochondrial control region sequences, microsatellites, and single nucleotide polymorphisms, or SNPs) to evaluate the population genetic structure of the species. The results were compared to those of previous studies of the species focusing on the Central Mediterranean and confirmed the presence of two divergent mtDNA haplogroups distributed without any detectable geographical structuring within the Mediterranean and the Atlantic. However, population analyses of the genetic structure of the species using either 10 microsatellites or a set of 1,051 SNP markers suggest that the species forms three genetically distinct groups, one in the Mediterranean and two in the Atlantic. The latter has so far not been reported in the Northeast Atlantic, and that this differentiation refers to samples from the Canary Islands is surprising. Fifteen candidate outlier SNP loci were identified in the data set, one located within the
tead1a
/TEF-1A gene, which has been associated with temperature acclimation of the medaka. This work enriches our knowledge of the genetic diversity of wild populations of the greater amberjack in the Mediterranean and the Northeast Atlantic and attempts to investigate signs of local adaptation toward a better understanding of the species distribution patterns. Future studies should be conducted to investigate the genomic regions associated with temperature acclimation in marine organisms that have to adapt to the ongoing climate change pressures, such as sea temperature rise, in order to survive.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

The greater amberjack (
Seriola dumerili
) has attracted considerable economic interest since the 1990s because it possesses traits that distinguish it as a promising candidate in aquaculture. However, the challenges of its successful rearing, combined with the need to develop proper management practices, underline the importance of research into the genetic diversity of both wild and farmed stocks. We aimed to decipher the genetic structure of
S. dumerili
in the Eastern Mediterranean and the Northeast Atlantic using three types of genetic markers (mitochondrial control region sequences, microsatellites, and single nucleotide polymorphisms, or SNPs) to evaluate the population genetic structure of the species. The results were compared to those of previous studies of the species focusing on the Central Mediterranean and confirmed the presence of two divergent mtDNA haplogroups distributed without any detectable geographical structuring within the Mediterranean and the Atlantic. However, population analyses of the genetic structure of the species using either 10 microsatellites or a set of 1,051 SNP markers suggest that the species forms three genetically distinct groups, one in the Mediterranean and two in the Atlantic. The latter has so far not been reported in the Northeast Atlantic, and that this differentiation refers to samples from the Canary Islands is surprising. Fifteen candidate outlier SNP loci were identified in the data set, one located within the
tead1a
/TEF-1A gene, which has been associated with temperature acclimation of the medaka. This work enriches our knowledge of the genetic diversity of wild populations of the greater amberjack in the Mediterranean and the Northeast Atlantic and attempts to investigate signs of local adaptation toward a better understanding of the species distribution patterns. Future studies should be conducted to investigate the genomic regions associated with temperature acclimation in marine organisms that have to adapt to the ongoing climate change pressures, such as sea temperature rise, in order to survive.

Fakriadis, Ioannis; Meiri-Ashkenazi, Iris; Bracha, Chen; Rosenfeld, Hanna; Corriero, Aldo; Zupa, Rosa; Pousis, Chrysovalentinos; Papadaki, Maria; Mylonas, Constantinos C

Gonadotropin expression, pituitary and plasma levels in the reproductive cycle of wild and captive-reared greater amberjack (Seriola dumerili) Journal Article

General and Comparative Endocrinology, 350 , pp. 114465, 2024, ISSN: 00166480.

Links | BibTeX

Ventriglia, G; Fakriadis, I; Papadaki, M; Zupa, R; Pousis, C; Mandalakis, M; Corriero, A; Mylonas, C C

Effects of different hormonal treatments on spermatogenesis advancement in hatchery-produced greater amberjack Seriola dumerili (Risso 1810) Journal Article

348 , pp. 114447, 2024, ISSN: 00166480.

Links | BibTeX

Lancerotto, Stefano; Fakriadis, Ioannis; Papadaki, Maria; Mandalakis, Manolis; Sigelaki, Irini; Mylonas, Constantinos C

Timing of puberty in F1-generation hatchery-produced greater amberjack (Seriola dumerili) Journal Article

General and Comparative Endocrinology, 347 , pp. 114414, 2024, ISSN: 00166480.

Links | BibTeX

2021

Fakriadis, Ioannis; Mylonas, Constantinos C

Sperm quality of greater amberjack Seriola dumerili throughout the reproductive season and in response to GnRHa treatment with controlled release implants Journal Article

Fish Physiology and Biochemistry, 47 (2), pp. 281–292, 2021, ISSN: 1573-5168.

Abstract | Links | BibTeX

@article{fakriadis_sperm_2021,
title = {Sperm quality of greater amberjack Seriola dumerili throughout the reproductive season and in response to GnRHa treatment with controlled release implants},
author = {Ioannis Fakriadis and Constantinos C Mylonas},
url = {https://doi.org/10.1007/s10695-020-00910-9},
doi = {10.1007/s10695-020-00910-9},
issn = {1573-5168},
year = {2021},
date = {2021-01-01},
urldate = {2021-04-27},
journal = {Fish Physiology and Biochemistry},
volume = {47},
number = {2},
pages = {281--292},
abstract = {A wild-caught stock of greater amberjack Seriola dumerili reared in sea cages in two commercial aquaculture facilities in Greece was administered gonadotropin-releasing hormone agonist (GnRHa), loaded in controlled release implants, and was used in spawning induction experiments throughout the reproductive season. Sperm quality was evaluated using computer-assisted sperm analysis (CASA) before and after GnRHa administration and the extent of the spermiation period was determined. Males were in spermiation throughout the monitoring period from May 30 until July 18, at temperatures between 19 and 24 °C. However, lower sperm motility duration, density, and survival under cold storage were observed from early July onwards. Sperm quality did not recover after the end of spawning induction experiments in tanks and the return of the fish for 14–28 days to the sea cage in mid-July, which could be related to the high temperatures of this period. An improvement trend was observed in the quality of the milt collected on day 7 after a single GnRHa administration, but a significant decrease was observed on day 21 in sperm density, survival under cold storage, and straight line velocity (VSL). On the contrary, a double GnRHa administration spaced 14 days apart maintained the same sperm quality for a longer period of 29 days. Further spermiation enhancement experiments should be conducted in the future to describe in more detail the kinetics of sperm production after GnRHa therapy and its effects on sperm quality.},
keywords = {},
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tppubtype = {article}
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Mello, Paulo De H; Lancerotto, Stefano; Fakriadis, Ioannis; Tsoukali, Panagiota; Papadaki, Maria; Mylonas, Constantinos Chrysanthos

The importance of thermoperiod for proper gametogenesis and successful egg and sperm production in meagre (Argyrosomus regius) breeders in aquaculture Journal Article

Mediterranean Marine Science, 22 (2), pp. 218–231, 2021, ISSN: 1791-6763.

Abstract | Links | BibTeX

@article{mello_importance_2021,
title = {The importance of thermoperiod for proper gametogenesis and successful egg and sperm production in meagre (Argyrosomus regius) breeders in aquaculture},
author = {Paulo De H Mello and Stefano Lancerotto and Ioannis Fakriadis and Panagiota Tsoukali and Maria Papadaki and Constantinos Chrysanthos Mylonas},
url = {https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/25806
https://imbbc.hcmr.gr/wp-content/uploads/2021/05/2021-De-Mello-MMS-27-2.pdf},
doi = {10.12681/mms.25806},
issn = {1791-6763},
year = {2021},
date = {2021-01-01},
urldate = {2021-04-27},
journal = {Mediterranean Marine Science},
volume = {22},
number = {2},
pages = {218--231},
abstract = {We examined the effect of constant water temperature throughout the year on gametogenesis, spawning success and egg/sperm/embryo quality in meagre (Argyrosomus regius). Two broodstocks were exposed to simulated natural photoperiod, andeither attenuated seasonal water temperature (SeasT, 16.4 to 19.6ºC) or relatively constant water temperature (CoT, 19.4 ± 0.6ºC).In the spawning period (May), 4 couples per group were induced to spawn with gonadotropin releasing hormone agonist (GnRHa). Gonadal stage of development, sperm quality parameters and plasma levels of sex steroids were evaluated prior to the GnRHa treatment. Spawning success and egg/sperm quality were examined over the following 4 weeks. Constant temperature did not prevent gametogenesis, but exposure to attenuated seasonal water temperature with the inclusion of winter low temperature was beneficial to both sexes. The mean (±SD) diameter of the largest vitellogenic oocytes prior to GnRHa administration was significantly higher in the SeasT compared to the CoT group (598 ± 27 vs 520 ± 17 μm). Testosterone plasma levels in the females were significantly higher in the SeasT group, but all other hormones were similar in both sexes. SeasT females spawned more consistently with higher relative fecundity, and 24-h embryo survival of the produced eggs. A more pronounced negative effect of constant water temperature was observed in males, since CoT males exhibited a spermiation index of 0 prior to GnRHa treatment, the latter clearly having a beneficial effect over the following 4 weeks. The study demonstrated that meagre do undergo gametogenesis to a significant extent even under constant water temperatures during the year. However, a seasonal thermal regime -even an attenuated one- was necessary for the proper development of the gametes, allowing for the successful spawning induction using the established GnRHa induction protocol.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Superio, Joshua; Fakriadis, Ioannis; Tsigenopoulos, Costas S; Lancerotto, Stefano Adam; Rodriguez, Andrea Villena; Vervelakis, Emanuele; Mylonas, Constantinos C

Spawning kinetics and parentage contribution of European sea bass (Dicentrarchus labrax) broodstocks, and influence of GnRHa-induced spawning Journal Article

Aquaculture Reports, 21 , pp. 100766, 2021, ISSN: 23525134.

Links | BibTeX

2020

Fakriadis, Ioannis; Zanatta, Eugenio Maria; Fleck, Renata Pontes Dos Santos; Mateo, Daybet Lorena Sena; Papadaki, Maria; Mylonas, Constantinos C

Endocrine regulation of long-term enhancement of spermiation in meagre (Argyrosomus regius) with GnRHa controlled-delivery systems Journal Article

General and Comparative Endocrinology, 297 , pp. 113549, 2020, ISSN: 00166480.

Links | BibTeX

Fakriadis, Ioannis; Miccoli, Andrea; Karapanagiotis, Stelios; Tsele, Nikoleta; Mylonas, Constantinos C

Optimization of a GnRHa treatment for spawning commercially reared greater amberjack Seriola dumerili: Dose response and extent of the reproductive season Journal Article

Aquaculture, 521 , 2020, ISSN: 00448486, (Type: Journal Article).

Links | BibTeX

Fakriadis, Ioannis; Sigelaki, Irini; Papadaki, Maria; Papandroulakis, Nikos; Raftopoulos, Anastasios; Tsakoniti, Kalliopi; Mylonas, Constantinos C

Control of reproduction of greater amberjack Seriola dumerili reared in aquaculture facilities Journal Article

Aquaculture, 519 , 2020, ISSN: 00448486, (Type: Journal Article).

Links | BibTeX

@article{fakriadis_control_2020,
title = {Control of reproduction of greater amberjack Seriola dumerili reared in aquaculture facilities},
author = {Ioannis Fakriadis and Irini Sigelaki and Maria Papadaki and Nikos Papandroulakis and Anastasios Raftopoulos and Kalliopi Tsakoniti and Constantinos C Mylonas},
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doi = {10.1016/j.aquaculture.2019.734880},
issn = {00448486},
year = {2020},
date = {2020-01-01},
journal = {Aquaculture},
volume = {519},
note = {Type: Journal Article},
keywords = {},
pubstate = {published},
tppubtype = {article}
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2019

Pérez, Evaristo; Linares, Fátima; Villanueva, José Luis Rodríguez; Vilar, Antonio; Mylonas, Constantinos C; Fakriadis, Ioannis; Papadaki, Maria; Papandroulakis, Nikos; Papadakis, Ioannis; Robles, Rocío; Fauvel, Christian; Roo, Javier; Peleteiro, José Benito; Lluch, Nuria; Pazos, Gema; Méndez, Belén; Sigelaki, Irini; Gómez, Castora; Pérez, Montse; Álvarez-Blázquez, Blanca

Wreckfish (Polyprion americanus). New Knowledge About Reproduction, Larval Husbandry, and Nutrition. Promise as a New Species for Aquaculture Journal Article

Fishes, 4 (1), pp. 14, 2019, (Type: Journal Article).

BibTeX

Forner-Piquer, Isabel; Fakriadis, Ioannis; Mylonas, Constantinos C; Piscitelli, Fabiana; Marzo, Vincenzo Di; Maradonna, Francesca; Calduch-Giner, Josep; Pérez-Sánchez, Jaume; Carnevali, Oliana

Effects of Dietary Bisphenol A on the Reproductive Function of Gilthead Sea Bream (Sparus aurata) Testes Journal Article

International Journal of Molecular Sciences, 20 (20), pp. 5003, 2019, (Type: Journal Article).

Links | BibTeX

Forner-Piquer, Isabel; Mylonas, Constantinos C; Fakriadis, Ioannis; Papadaki, Maria; Piscitelli, Fabiana; Marzo, Vincenzo Di; Calduch-Giner, Josep; Pérez-Sánchez, Jaume; Carnevali, Oliana

Effects of diisononyl phthalate (DiNP) on the endocannabinoid and reproductive systems of male gilthead sea bream (Sparus aurata) during the spawning season Journal Article

Archives of Toxicology, 2019, ISSN: 1432-0738, (Type: Journal Article).

Links | BibTeX

Fakriadis, I; Lisi, F; Sigelaki, I; Papadaki, M; Mylonas, C C

Spawning kinetics and egg/larval quality of greater amberjack (Seriola dumerili) in response to multiple GnRHa injections or implants Journal Article

General and Comparative Endocrinology, 279 , pp. 78–87, 2019, ISSN: 1095-6840 (Electronic) 0016-6480 (Linking), (Type: Journal Article).

Links | BibTeX

2018

Pousis, C; Mylonas, C C; Virgilio, De C; Gadaleta, G; Santamaria, N; Passantino, L; Zupa, R; Papadaki, M; Fakriadis, I; Ferreri, R; Corriero, A

The observed oogenesis impairment in greater amberjack Seriola dumerili (Risso, 1810) reared in captivity is not related to an insufficient liver transcription or oocyte uptake of vitellogenin Journal Article

Aquaculture Research, 49 (1), pp. 243–252, 2018, ISSN: 1355557X, (Publisher: Blackwell Publishing Ltd).

Abstract | Links | BibTeX

@article{pousis_observed_2018,
title = {The observed oogenesis impairment in greater amberjack Seriola dumerili (Risso, 1810) reared in captivity is not related to an insufficient liver transcription or oocyte uptake of vitellogenin},
author = {C Pousis and C C Mylonas and C De Virgilio and G Gadaleta and N Santamaria and L Passantino and R Zupa and M Papadaki and I Fakriadis and R Ferreri and A Corriero},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85026499054&doi=10.1111%2fare.13453&partnerID=40&md5=ecb8f6c6b9bbbf8b803322299447cfd4},
doi = {10.1111/are.13453},
issn = {1355557X},
year = {2018},
date = {2018-01-01},
journal = {Aquaculture Research},
volume = {49},
number = {1},
pages = {243--252},
abstract = {The greater amberjack Seriola dumerili is an excellent candidate for the Mediterranean aquaculture, due to its large body size and high growth rate, as well as its high flesh quality and commercial value worldwide. For its successful incorporation in the aquaculture industry, an in-depth understanding of the reproductive function of the species under rearing conditions is necessary, since completion of oogenesis in captivity is currently a bottleneck for the commercial production of the species. Liver and ovary samples from wild and captive-reared greater amberjack females were collected at three different phases of the reproductive cycle: early gametogenesis (EARLY, late April-early May), advanced gametogenesis (ADVANCED, late May-early June) and spawning (SPAWNING, late June-July). The cDNAs of three vitellogenins (VtgA, VtgB and VtgC) were partially sequenced and a qRT-PCR for their expression was used to compare ovarian maturity stage and liver vitellogenin transcript levels between wild and captive-reared individuals. An extensive atresia of late vitellogenic follicles, which prevented any further oocyte development and spawning was observed in captive-reared individuals during the ADVANCED phase. The expression levels of the three vitellogenins, as well as the amount of yolk globules in vitellogenic oocytes, did not differ significantly between captive-reared and wild females, indicating that the observed oogenesis impairment in greater amberjack reared in captivity was not related to an insufficient liver synthesis or a reduced oocyte uptake of vitellogenin. © 2017 John Wiley & Sons Ltd},
note = {Publisher: Blackwell Publishing Ltd},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Papadaki, M; Mazzella, D; Santinelli, V; Fakriadis, I; Sigelaki, I; Mylonas, C C

Hermaphroditism and reproductive function of hatchery-produced sharpsnout seabream (Diplodus puntazzo) under attenuated annual thermal cycles Journal Article

Aquaculture, 482 , pp. 231–240, 2018, ISSN: 00448486, (Publisher: Elsevier B.V.).

Abstract | Links | BibTeX

@article{papadaki_hermaphroditism_2018,
title = {Hermaphroditism and reproductive function of hatchery-produced sharpsnout seabream (Diplodus puntazzo) under attenuated annual thermal cycles},
author = {M Papadaki and D Mazzella and V Santinelli and I Fakriadis and I Sigelaki and C C Mylonas},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85030865182&doi=10.1016%2fj.aquaculture.2017.09.028&partnerID=40&md5=61ad82357d509cb8f19491299ca570e0},
doi = {10.1016/j.aquaculture.2017.09.028},
issn = {00448486},
year = {2018},
date = {2018-01-01},
journal = {Aquaculture},
volume = {482},
pages = {231--240},
abstract = {Acquiring more knowledge on the reproductive biology of the sharpsnout seabream (Diplodus puntazzo) can improve our ability to control its reproduction and enable hatcheries to reconsider this fish as a potential species for the Mediterranean aquaculture production. We investigated hermaphroditism and reproductive function in a hatchery-produced broodstock over a long period of 6 years, which includes sex differentiation, puberty and four consecutive annual reproductive seasons, at relatively constant water temperatures of 18–20 °C, which are typical of borehole seawater supplies used frequently in marine hatcheries for biosecurity reasons. At the end of the first year of life, the gonads were bisexual and were classified into three different categories: mainly testicular tissue with rudimentary ovarian tissue (Mf), substantial amounts of both tissues (MF) and mainly ovarian tissue with rudimentary testicular tissue (mF). Fully female (F) gonads appeared already in 1 + years old fish, whereas fully male (M) gonads appeared for the first time in 2 + years old fish. The sex ratio (F:M) of the studied populations during the first 6 years of life in all age classes was not different from 1:1. Therefore, no annual restructuring of the broodstock is necessary in order to avoid the development of skewed sex ratios, since once functional sex is established it does not seem to change in the following years. Furthermore, the use of attenuated annual thermal cycling regimes did not seem to affect the annual gametogenic cycle and full maturation of this species, though spontaneous spawning was still missing and the fish exhibited the typical problems reported for this species in captivity. © 2017 Elsevier B.V.},
note = {Publisher: Elsevier B.V.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Jerez, Salvador; Fakriadis, Ioannis; Papadaki, Maria; Martín, M; Cejas, Juana; Mylonas, Constantinos C

Spawning induction of first-generation (F1) greater amberjack Seriola dumerili in the Canary Islands, Spain using GnRHa delivery systems Journal Article

Fishes, 3 (3), pp. 1–22, 2018, ISSN: 2410-3888, (Type: Journal Article).

Links | BibTeX

Papadaki, Maria; Peleteiro, José Benito; Alvarez-Blázquez, Blanca; Villanueva, José Luis Rodríguez; Linares, Fatima; Vilar, Antonio; Rial, Evaristo Pérez; Lluch, Nuria; Fakriadis, Ioannis; Sigelaki, Irini; Mylonas, Constantinos C

Description of the Annual Reproductive Cycle of Wreckfish Polyprion americanus in Captivity Journal Article

Fishes, 3 (4), pp. 1–20, 2018, ISSN: 2410-3888, (Type: Journal Article).

Links | BibTeX

2017

Zupa, R; Rodrõâguez, C; Mylonas, C C; Rosenfeld, H; Fakriadis, I; Papadaki, M; Peârez, J A; Pousis, C; Basilone, G; Corriero, A

Comparative study of reproductive development in wild and captive-reared greater amberjack seriola dumerili (Risso, 1810) Journal Article

PLoS ONE, 12 (1), 2017, ISSN: 19326203, (Publisher: Public Library of Science).

Abstract | Links | BibTeX

@article{zupa_comparative_2017,
title = {Comparative study of reproductive development in wild and captive-reared greater amberjack seriola dumerili (Risso, 1810)},
author = {R Zupa and C Rodrõâguez and C C Mylonas and H Rosenfeld and I Fakriadis and M Papadaki and J A Peârez and C Pousis and G Basilone and A Corriero},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85008686824&doi=10.1371%2fjournal.pone.0169645&partnerID=40&md5=09301e14b368a835887bf0e7176db149},
doi = {10.1371/journal.pone.0169645},
issn = {19326203},
year = {2017},
date = {2017-01-01},
journal = {PLoS ONE},
volume = {12},
number = {1},
abstract = {The greater amberjack Seriola dumerili is a large teleost fish with rapid growth and excellent flesh quality, whose domestication represents an ambitious challenge for aquaculture. The occurrence of reproductive dysfunctions in greater amberjack reared in captivity was investigated by comparing reproductive development of wild and captive-reared individuals. Wild and captive-reared breeders were sampled in the Mediterranean Sea during three different phases of the reproductive cycle: Early gametogenesis (EARLY, late April-early May), advanced gametogenesis (ADVANCED, late May-early June) and spawning (SPAWNING, late June-July). Fish reproductive state was evaluated using the gonado-somatic index (GSI), histological analysis of the gonads and determination of sex steroid levels in the plasma, and correlated with leptin expression in the liver and gonad biochemical composition. The GSI and sex steroid levels were lower in captive-reared than in wild fish. During the ADVANCED period, when the wild greater amberjack breeders were already in spawning condition, ovaries of captive-reared breeders showed extensive atresia of late vitellogenic oocytes and spermatogenic activity ceased in the testes of half of the examined males. During the SPAWNING period, all captive-reared fish had regressed gonads, while wild breeders still displayed reproductive activity. Liver leptin expression and gonad proximate composition of wild and captive greater amberjack were similar. However, the gonads of captive-reared fish showed different total polar lipid contents, as well as specific lipid classes and fatty acid profiles with respect to wild individuals. This study underlines the need for an improvement in rearing technology for this species, which should include minimum handling during the reproductive season and the formulation of a specific diet to overcome the observed gonadal decrements of phospholipids, DHA (22:6n-3) and ARA (20:4n-6), compared to wild breeders. © 2017 Zupa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},
note = {Publisher: Public Library of Science},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

The greater amberjack Seriola dumerili is a large teleost fish with rapid growth and excellent flesh quality, whose domestication represents an ambitious challenge for aquaculture. The occurrence of reproductive dysfunctions in greater amberjack reared in captivity was investigated by comparing reproductive development of wild and captive-reared individuals. Wild and captive-reared breeders were sampled in the Mediterranean Sea during three different phases of the reproductive cycle: Early gametogenesis (EARLY, late April-early May), advanced gametogenesis (ADVANCED, late May-early June) and spawning (SPAWNING, late June-July). Fish reproductive state was evaluated using the gonado-somatic index (GSI), histological analysis of the gonads and determination of sex steroid levels in the plasma, and correlated with leptin expression in the liver and gonad biochemical composition. The GSI and sex steroid levels were lower in captive-reared than in wild fish. During the ADVANCED period, when the wild greater amberjack breeders were already in spawning condition, ovaries of captive-reared breeders showed extensive atresia of late vitellogenic oocytes and spermatogenic activity ceased in the testes of half of the examined males. During the SPAWNING period, all captive-reared fish had regressed gonads, while wild breeders still displayed reproductive activity. Liver leptin expression and gonad proximate composition of wild and captive greater amberjack were similar. However, the gonads of captive-reared fish showed different total polar lipid contents, as well as specific lipid classes and fatty acid profiles with respect to wild individuals. This study underlines the need for an improvement in rearing technology for this species, which should include minimum handling during the reproductive season and the formulation of a specific diet to overcome the observed gonadal decrements of phospholipids, DHA (22:6n-3) and ARA (20:4n-6), compared to wild breeders. © 2017 Zupa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Zupa, R; Fauvel, C; Mylonas, C C; Pousis, C; Santamaria, N; Papadaki, Μ; Fakriadis, I; Cicirelli, V; Mangano, S; Passantino, L; Lacalandra, G M; Corriero, A

Rearing in captivity affects spermatogenesis and sperm quality in greater amberjack, Seriola dumerili (Risso, 1810) Journal Article

Journal of Animal Science, 95 (9), pp. 4085–4100, 2017, ISSN: 00218812, (Publisher: American Society of Animal Science).

Abstract | Links | BibTeX

@article{zupa_rearing_2017,
title = {Rearing in captivity affects spermatogenesis and sperm quality in greater amberjack, Seriola dumerili (Risso, 1810)},
author = {R Zupa and C Fauvel and C C Mylonas and C Pousis and N Santamaria and Μ Papadaki and I Fakriadis and V Cicirelli and S Mangano and L Passantino and G M Lacalandra and A Corriero},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85029717001&doi=10.2527%2fjas.2017.1708&partnerID=40&md5=93369a73080df336a351b6a9a006cab1},
doi = {10.2527/jas.2017.1708},
issn = {00218812},
year = {2017},
date = {2017-01-01},
journal = {Journal of Animal Science},
volume = {95},
number = {9},
pages = {4085--4100},
abstract = {The greater amberjack, Seriola dumerili (Risso, 1810), is a promising candidate for the diversification of European aquaculture production, but inconsistent reproduction in captivity prevents commercial production. Recent studies showed that greater amberjack confined in sea cages exhibited scarce gonad development and early interruption of gametogenic activity during the reproductive season. The aim of the present study was to improve our understanding of the observed impairment of spermatogenesis. Adult wild and captive-reared males were sampled during 3 different phases of the reproductive cycle: early gametogenesis (EARLY; late April to early May), advanced gametogenesis (ADVANCED; late May to early June), and spawning (SPAWNING; late June to July). Spermatogonial stem cells and proliferating germ cells were identified through the immunohistochemical localization of Pou5f1 and proliferating cell nuclear antigen, respectively. Apoptotic germ cells were identified throughout the terminal deoxynucleotidyl transferase-mediated 2’-deoxyuridine 5’-triphosphate nick end labeling method. Sperm quality of captive-reared fish was evaluated using computer-assisted sperm analysis. Captive-reared males exhibited seminiferous lobules of a smaller diameter, a precocious and progressive decrease of spermatogonial mitosis, and a high level of apoptosis at the beginning of the reproductive season, concomitant with a many-fold higher 17β-estradiol plasma concentration. The motile spermatozoa percentage of captive greater amberjack was lower than in other teleosts, and a drastic decrease of spermatozoa motility duration, velocity, and ATP content occurred along the reproductive season. An abnormal increase of sperm concentration as well as an increase of dead spermatozoa occurred during the SPAWNING phase, probably because of lack of sperm hydration and ejaculation and consequent sperm ageing. The present study demonstrates the extreme susceptibility of greater amberjack to rearing stress and underscores the need for improvement of the rearing and handling procedures to ameliorate gametogenesis dysfunctions in commercial aquaculture production. © 2017 American Society of Animal Science. All rights reserved.},
note = {Publisher: American Society of Animal Science},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

The greater amberjack, Seriola dumerili (Risso, 1810), is a promising candidate for the diversification of European aquaculture production, but inconsistent reproduction in captivity prevents commercial production. Recent studies showed that greater amberjack confined in sea cages exhibited scarce gonad development and early interruption of gametogenic activity during the reproductive season. The aim of the present study was to improve our understanding of the observed impairment of spermatogenesis. Adult wild and captive-reared males were sampled during 3 different phases of the reproductive cycle: early gametogenesis (EARLY; late April to early May), advanced gametogenesis (ADVANCED; late May to early June), and spawning (SPAWNING; late June to July). Spermatogonial stem cells and proliferating germ cells were identified through the immunohistochemical localization of Pou5f1 and proliferating cell nuclear antigen, respectively. Apoptotic germ cells were identified throughout the terminal deoxynucleotidyl transferase-mediated 2’-deoxyuridine 5’-triphosphate nick end labeling method. Sperm quality of captive-reared fish was evaluated using computer-assisted sperm analysis. Captive-reared males exhibited seminiferous lobules of a smaller diameter, a precocious and progressive decrease of spermatogonial mitosis, and a high level of apoptosis at the beginning of the reproductive season, concomitant with a many-fold higher 17β-estradiol plasma concentration. The motile spermatozoa percentage of captive greater amberjack was lower than in other teleosts, and a drastic decrease of spermatozoa motility duration, velocity, and ATP content occurred along the reproductive season. An abnormal increase of sperm concentration as well as an increase of dead spermatozoa occurred during the SPAWNING phase, probably because of lack of sperm hydration and ejaculation and consequent sperm ageing. The present study demonstrates the extreme susceptibility of greater amberjack to rearing stress and underscores the need for improvement of the rearing and handling procedures to ameliorate gametogenesis dysfunctions in commercial aquaculture production. © 2017 American Society of Animal Science. All rights reserved.

2016

Flack, A; Fiedler, W; Blas, J; Pokrovsky, I; Kaatz, M; Mitropolsky, M; Aghababyan, K; Fakriadis, I; Makrigianni, E; Jerzak, L; Azafzaf, H; Feltrup-Azafzaf, C; Rotics, S; Mokotjomela, T M; Nathan, R; Wikelski, M

Costs of migratory decisions: A comparison across eight white stork populations Journal Article

Science Advances, 2 (1), 2016, ISSN: 23752548, (Publisher: American Association for the Advancement of Science).

Abstract | Links | BibTeX

@article{flack_costs_2016,
title = {Costs of migratory decisions: A comparison across eight white stork populations},
author = {A Flack and W Fiedler and J Blas and I Pokrovsky and M Kaatz and M Mitropolsky and K Aghababyan and I Fakriadis and E Makrigianni and L Jerzak and H Azafzaf and C Feltrup-Azafzaf and S Rotics and T M Mokotjomela and R Nathan and M Wikelski},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84974545850&doi=10.1126%2fsciadv.1500931&partnerID=40&md5=512900f1a33f4132dce13117d925ce50},
doi = {10.1126/sciadv.1500931},
issn = {23752548},
year = {2016},
date = {2016-01-01},
journal = {Science Advances},
volume = {2},
number = {1},
abstract = {Annual migratory movements can range from a few tens to thousands of kilometers, creating unique energetic requirements for each specific species and journey. Even within the same species, migration costs can vary largely because of flexible, opportunistic life history strategies. We uncover the large extent of variation in the lifetime migratory decisions of young white storks originating from eight populations. Not only did juvenile storks differ in their geographically distinct wintering locations, their diverse migration patterns also affected the amount of energy individuals invested for locomotion during the first months of their life. Overwintering in areas with higher human population reduced the stork's overall energy expenditure because of shorter daily foraging trips, closer wintering grounds, or a complete suppression of migration. Because migrants can change ecological processes in several distinct communities simultaneously, understanding their life history decisions helps not only to protect migratory species but also to conserve stable ecosystems. © The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science.},
note = {Publisher: American Association for the Advancement of Science},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Mylonas, C C; Salone, S; Biglino, T; de Mello, P H; Fakriadis, I; Sigelaki, I; Duncan, N

Enhancement of oogenesis/spermatogenesis in meagre Argyrosomus regius using a combination of temperature control and GnRHa treatments Journal Article

Aquaculture, 464 , pp. 323–330, 2016, ISSN: 00448486, (Publisher: Elsevier B.V.).

Abstract | Links | BibTeX

@article{mylonas_enhancement_2016,
title = {Enhancement of oogenesis/spermatogenesis in meagre Argyrosomus regius using a combination of temperature control and GnRHa treatments},
author = {C C Mylonas and S Salone and T Biglino and P H de Mello and I Fakriadis and I Sigelaki and N Duncan},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84978193838&doi=10.1016%2fj.aquaculture.2016.07.006&partnerID=40&md5=33e0a7321455743f1b6c84fcc8aedb38},
doi = {10.1016/j.aquaculture.2016.07.006},
issn = {00448486},
year = {2016},
date = {2016-01-01},
journal = {Aquaculture},
volume = {464},
pages = {323--330},
abstract = {A multiple GnRHa injection method for inducing spawning of high fecundity and quality in meagre was optimized. Specifically, the study (a) examined how long females would continue spawning in response to consecutive, weekly GnRHa injections, if maintained at the natural spawning temperature, and (b) evaluated whether males can produce adequate amounts of good quality sperm for the same period, with the assistance of a GnRHa therapy. Combined with stable temperatures (19–20 °C) that are prevalent during the early spawning season (April–May) in the Mediterranean Sea, each weekly GnRHa injection induced 2 consecutive spawns per week for a period of 17 weeks. Fish spawned consistently 2 days after treatment and produced high fecundity and egg quality (i.e. fertilization, hatching and early larval development). Maximum mean total fecundity (± S.D.) obtained was 1,415,000 ± 149,000 eggs kg− 1 in 32 spawns, being the highest total season fecundity reported for meagre, and significantly higher than the estimated maximum potential fecundity for the species. At the same time, sperm of consistently high quality (i.e. initial motility, duration of motility and storage survival) was produced in response to treatment with multiple GnRHa implants, though towards the end of the experiment a significant reduction (ANOVA, P ≤ 0.05) in spermatozoa density was observed. This protocol is expected to enhance greatly commercial production of meagre. Statement of relevance The present study reports on an optimized protocol for the induction of consistent and repeated spawning in meagre, based on the use of weekly GnRHa injections combined with stable temperatures that are prevalent at the early spawning season in the Mediterranean Sea. This protocol produced eggs of high fecundity and quality (i.e. fertilization, hatching and early larval development) for a period of > 4 months, and it is expected to enhance greatly commercial production of meagre. © 2016},
note = {Publisher: Elsevier B.V.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

2008

Peñaranda, D S; Pérez, L; Fakriadis, G; Mylonas, C C; Asturiano, J F

Effects of extenders and cryoprotectant combinations on motility and morphometry of sea bass (Dicentrarchus labrax) spermatozoa Journal Article

Journal of Applied Ichthyology, 24 (4), pp. 450–455, 2008, ISSN: 01758659.

Abstract | Links | BibTeX

1862 entries « ‹ 1 of 38 › »

2025

Idan, Tal; Shefer, Sigal; Chatzigeorgiou, Giorgos; Gerovasileiou, Vasilis; Goren, Liron

Testing the effect of host availability on endobiont diversity: proposing the single hotel hypothesis Journal Article

Scientific Reports, 15 (1), pp. 21717, 2025, ISSN: 2045-2322.

Links | BibTeX

Wylie, Matthew J; Ribeiro, Flavio F; Fantham, Warren; Puklowski, Morgan E; Rebstock, Ria; Shaw, Nicola; Mylonas, Constantinos C; Kato, Keitaro; Elizur, Abigail; Wellenreuther, Maren

Effects of hormonal treatments on spawning latency and egg production of captive silver trevally (Pseudocaranx georgianus) Journal Article

Aquaculture Reports, 43 , pp. 102964, 2025, ISSN: 23525134.

Links | BibTeX

Möller, Ben; Livanou, Eleni; Psarra, Stella; Koulouri, Panayota; Dounas, Costas; Rotter, Ana; Mandalakis, Manolis

Comparative assessment of pelagic/benthic microalgae in the oligotrophic continental shelf of Heraklion Bay, Eastern Mediterranean: A pigments-based chemotaxonomic analysis Journal Article

Regional Studies in Marine Science, 89 , pp. 104320, 2025, ISSN: 23524855.

Links | BibTeX

Kotsiri, Mado; Kogiannou, Dimitra; Nikoloudaki, Chrisanthi; Kleidas, Ioannis; Dogrammatzi, Aikaterini; Karachle, Paraskevi K; Grigorakis, Kriton

Seasonal Variation in Nutritional Value and Technical Quality of Lionfish (Pterois miles) from the Ionian and Aegean Seas Journal Article

Foods, 14 (13), pp. 2353, 2025, ISSN: 2304-8158.

Abstract | Links | BibTeX

@article{kotsiri_seasonal_2025,
title = {Seasonal Variation in Nutritional Value and Technical Quality of Lionfish (Pterois miles) from the Ionian and Aegean Seas},
author = {Mado Kotsiri and Dimitra Kogiannou and Chrisanthi Nikoloudaki and Ioannis Kleidas and Aikaterini Dogrammatzi and Paraskevi K Karachle and Kriton Grigorakis},
url = {https://www.mdpi.com/2304-8158/14/13/2353
/wp-content/uploads/2025/07/2025-Kotsiri-foods-37.pdf
},
doi = {10.3390/foods14132353},
issn = {2304-8158},
year = {2025},
date = {2025-07-18},
urldate = {2025-07-18},
journal = {Foods},
volume = {14},
number = {13},
pages = {2353},
abstract = {Lionfish (Pterois miles), an invasive species in the Mediterranean, pose ecological and socioeconomic challenges. This study examines the seasonal variation in the nutritional composition and technical quality of lionfish from the Ionian and Aegean Seas, evaluating their potential as a food resource. Fillets were high in protein (19.4%) and low in fat (2.0%), with significant seasonal differences in the Ionian Sea, where winter samples had higher lipid content. The fillet yield (28.4%) was satisfactory given the fish’s morphology. Fatty acid analysis confirmed lionfish as a valuable source of polyunsaturated fatty acids (PUFAs), particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), with EPA + DHA levels exceeding the recommended daily intake (119.2%). Seasonal variations in fatty acid composition were observed, including higher EPA in autumn and lower lipid nutritional quality in summer. Arachidonic acid (ARA) was also present at nutritionally significant levels (79.4 mg/100 g). The n3/n6 ratio (2.2) and favourable atherogenic and thrombogenic indices highlight its nutritional benefits. This is the first study to assess seasonal variations in the nutritional value and technical yield of lionfish in the Mediterranean, offering novel insights into its commercial valorisation. These findings support its promotion as a sustainable protein source and as a means of managing its invasive population.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Manga, Michael; Tominaga, Masako; Preine, Jonas; Ronge, Thomas A; Beethe, Sarah; Hübscher, Christian; McIntosh, Iona; Nomikou, Paraskevi; Kutterolf, Steffen; Druitt, Tim; Bernard, Alexis; Berthod, Carole; Chen, Hehe; Clark, Acacia; DeBari, Susan; Fernandez‐Perez, Tatiana I; Gertisser, Ralf; Johnston, Raymond M; Jones, Christopher K; Joshi, Batuk K; Kletetschka, Günther; Koukousioura, Olga; McCanta, Molly; Morris, Antony; Pank, Katharina; Peccia, Ally; Polymenakou, Paraskevi N; Woodhouse, Adam; Yamamoto, Yuzuru

Low Heat Flow in the Anhydros Basin, Aegean Sea, Recorded by Deep Subsurface Temperatures Journal Article

Geophysical Research Letters, 52 (13), pp. e2025GL115919, 2025, ISSN: 0094-8276, 1944-8007.

Abstract | Links | BibTeX

@article{manga_low_2025,
title = {Low Heat Flow in the Anhydros Basin, Aegean Sea, Recorded by Deep Subsurface Temperatures},
author = {Michael Manga and Masako Tominaga and Jonas Preine and Thomas A Ronge and Sarah Beethe and Christian Hübscher and Iona McIntosh and Paraskevi Nomikou and Steffen Kutterolf and Tim Druitt and Alexis Bernard and Carole Berthod and Hehe Chen and Acacia Clark and Susan DeBari and Tatiana I Fernandez‐Perez and Ralf Gertisser and Raymond M Johnston and Christopher K Jones and Batuk K Joshi and Günther Kletetschka and Olga Koukousioura and Molly McCanta and Antony Morris and Katharina Pank and Ally Peccia and Paraskevi N Polymenakou and Adam Woodhouse and Yuzuru Yamamoto},
url = {https://agupubs.onlinelibrary.wiley.com/doi/10.1029/2025GL115919
/wp-content/uploads/2025/07/2025-Manga-GRL-36.pdf},
doi = {10.1029/2025GL115919},
issn = {0094-8276, 1944-8007},
year = {2025},
date = {2025-07-16},
urldate = {2025-07-16},
journal = {Geophysical Research Letters},
volume = {52},
number = {13},
pages = {e2025GL115919},
abstract = {Abstract

Subseafloor in situ temperatures in a drilled hole in the Anhydros Basin, Aegean Sea, measured during International Ocean Discovery Program (IODP) Expedition 398, yielded a low heat flow (textless0.023 W/m
2
) despite active magmatism and rifting in the region. The coldest and highest temperatures were 13.9°C at 52.5 m below seafloor (mbsf) and 15.5°C for the deepest measurement at 360.4 mbsf, respectively. Comparison of a heat transfer model with measurements suggests that sea bottom temperatures during the last glacial period were up to 10°C cooler than Holocene temperatures. The magnitude of Holocene warming co‐varies with the geothermal heat flow: if the former goes up the latter goes up. Low heat flow may arise from lateral removal of heat through deeper formations by gravity driven advection of fluids. Tectonic separation of the northwestern Anhydros Basin from the Christiana‐Santorini‐Kolumbo volcanic field may lead to minimal magmatic influences on heat flow.

,
Plain Language Summary
Temperatures in Earth's crust reveal the processes that create and transport heat. In the rifting Anhydros Basin in the Aegean Sea, north of the active South Aegean Volcanic Arc, International Ocean Discovery Program Expedition 398 measured cold subsurface temperatures in a borehole to depths exceeding 300 m below the seafloor. These temperatures record low heat flow and cold seafloor temperatures from the last glacial period. The low heat flow at shallow depths may be due to deeper fluid circulation that removes heat. Low heat flow further implies that there are no magma bodies within the crust in the northwestern part of the Anhydros Basin.
,
Key Points

Heat flow is low in the Anhydros Basin, Aegean Sea

Ocean bottom temperatures were cold during the last glacial

Subsurface temperature records surface temperature changes and heat flow},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Dritsas, Panagiotis; Patsialou, Stefania; Kampantais, Dimitrios; Roussos, Efstratios; Kotzamanis, Yannis; Tekerlekopoulou, Athanasia; Vayenas, Dimitris V; Aggelis, George

Investigating the Potential of Newly Isolated Microalgae Strains from the Ionian Sea (Greece) Cultured in an Open Raceway Pond Journal Article

Applied Sciences, 15 (12), pp. 6680, 2025, ISSN: 2076-3417.

Abstract | Links | BibTeX

@article{dritsas_investigating_2025,
title = {Investigating the Potential of Newly Isolated Microalgae Strains from the Ionian Sea (Greece) Cultured in an Open Raceway Pond},
author = {Panagiotis Dritsas and Stefania Patsialou and Dimitrios Kampantais and Efstratios Roussos and Yannis Kotzamanis and Athanasia Tekerlekopoulou and Dimitris V Vayenas and George Aggelis},
url = {https://www.mdpi.com/2076-3417/15/12/6680
/wp-content/uploads/2025/07/2025-Dritsas-AppSci-35.pdf},
doi = {10.3390/app15126680},
issn = {2076-3417},
year = {2025},
date = {2025-07-07},
urldate = {2025-07-10},
journal = {Applied Sciences},
volume = {15},
number = {12},
pages = {6680},
abstract = {Microalgae are a subject of interest not only for fundamental research but for various biotechnological applications as well. In this study, the ability of newly isolated strains, i.e., Picochlorum costavermella VAS2.5, Picochlorum oklahomense SAG4.4, Microchloropsis gaditana VON5.3, and Nephroselmis pyriformis PAT2.7, to grow when cultured in an open raceway pond under laboratory conditions and produce various metabolites of high-added value was evaluated. N. pyriformis PAT2.7 and P. costavermella VAS2.5 were the greatest in biomass production (exceeding 0.4 g/L), while P. costavermella VAS2.5 and M. gaditana VON5.3 were the greatest in lipid production (reaching approximately 18%, wt/wt). The lipid fraction of glycolipids and sphingolipids was predominant (43.6–55.4%, wt/wt), followed by neutral lipids (27.1–50.1%, wt/wt) and phospholipids (6.9–17.4%, wt/wt). Picochlora and M. gaditana VON5.3 lipids were rich in Δ5,8,11,14,17C20:5 and/or Δ9,12,15C18:3, while N. pyriformis PAT2.7 synthesized Δ9C16:1 in large quantities (30–40%, wt/wt). All strains showed remarkable yields in polysaccharide and protein production, demonstrated a well-balanced amino acid profile, and synthesized pigments in amounts comparable to other studies. The biochemical profiles of these strains showcased their suitability for use primarily in the aquaculture sector.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Vasilaki, Antigoni; Nengas, Ioannis; Fountoulaki, Eleni; Henry, Morgane; Kogiannou, Dimitra; Nikoloudaki, Chrysanthi; Chronopoulos, Petros; Karapanagiotidis, Ioannis T; Mente, Elena

Nutritional enhancement of lupin meal (Lupinus albus), through fermentation with textitSaccharomyces cerevisiae, as plant protein ingredient in aquafeeds for the European sea bass (Dicentrarchus labrax). Journal Article

Aquaculture, 609 , pp. 742820, 2025, ISSN: 0044-8486.

Abstract | Links | BibTeX

@article{vasilaki_nutritional_2025,
title = {Nutritional enhancement of lupin meal (\textit{Lupinus albus}), through fermentation with textitSaccharomyces cerevisiae, as plant protein ingredient in aquafeeds for the European sea bass (\textit{Dicentrarchus labrax}).},
author = {Antigoni Vasilaki and Ioannis Nengas and Eleni Fountoulaki and Morgane Henry and Dimitra Kogiannou and Chrysanthi Nikoloudaki and Petros Chronopoulos and Ioannis T Karapanagiotidis and Elena Mente},
url = {https://www.sciencedirect.com/science/article/pii/S0044848625007069
/wp-content/uploads/2025/07/2025-Vasilaki-AQUA-34.pdf},
doi = {10.1016/j.aquaculture.2025.742820},
issn = {0044-8486},
year = {2025},
date = {2025-07-04},
urldate = {2025-07-09},
journal = {Aquaculture},
volume = {609},
pages = {742820},
abstract = {Legumes contain antinutritional factors that restrict their use in fish nutrition, however, biotechnological methods such as solid-state fermentation can improve their nutritional profile, positioning them as a sustainable alternative for aquafeed formulation. This research evaluated the feasibility of replacing soybean meal with Saccharomyces cerevisiae-fermented lupin meal (Lupinus albus) in feed formulations for European sea bass (Dicentrarchus labrax). Four diets were produced; one commercial type diet (FRL0) and three diets with partial (FRL10, FRL12.5) or complete (FRL15) replacement of soybean meal by fermented lupin meal. European sea bass juveniles, with an initial average weight: 18.9 g, were fed the experimental diets for 71 days in triplicate groups. Results indicated no adverse effects on key production indices when soybean meal was replaced partially or totally. Fermented lupin meal linearly improved feed utilization. Apparent digestibility coefficient of protein significantly increased in groups fed 12.5 % and 15 % fermented lupin, as did apparent digestibility coefficient of fat, compared to the FRL0 group. Trypsin activity in pyloric caeca was statistically elevated in the fermented lupin groups compare to FRL0 group. Whole-body composition, amino acid content, and deposition showed no significant differences among groups. Fermented lupin did not suppress the immune system, and a slight immunostimulatory effect was observed at a 12.5 % inclusion level. No histopathological alterations were detected. Overall, the results demonstrated the efficacy of solid-state fermentation process in enhancing lupin meal, highlighting its potential as a sustainable replacement for soybean meal in feed formulations for Mediterranean marine aquaculture species such us D. labrax.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Droubogiannis, Stavros; Triga, Andriana; Pavlidis, Michail; Katharios, Pantelis

Genome sequences of textitVibrio harveyi VH2 and textitVibrio harveyi Vhp1-sp Journal Article

Microbiology Resource Announcements, pp. e00087–25, 2025, ISSN: 2576-098X.

Abstract | Links | BibTeX

Lancerotto, Stefano; Fakriadis, Ioannis; Papadaki, Maria; Sigelaki, Irini; Giménez, Ignacio; Genoves, Jose Vicente Roig; Meloni, Andrea; Mylonas, Constantinos C

Aquaculture, 609 , pp. 742795, 2025, ISSN: 00448486.

Links | BibTeX

Keklikoglou, Kleoniki; Mandalakis, Manolis; Fanouraki, Eleftheria; Anastasiou, Thekla I; Kagiampaki, Eirini; Vasileiadou, Katerina; Pavloudi, Christina; Arvanitidis, Christos; Pavlidis, Michail

Disrupted darkness: the impact of anthropogenic light at night on melatonin secretion of Hermodice carunculata (Polychaeta, Annelida) Journal Article

Photochemical & Photobiological Sciences, 24 (6), pp. 1031–1040, 2025, ISSN: 1474-905X, 1474-9092.

Abstract | Links | BibTeX

@article{keklikoglou_disrupted_2025,
title = {Disrupted darkness: the impact of anthropogenic light at night on melatonin secretion of \textit{Hermodice carunculata (Polychaeta, Annelida)}},
author = {Kleoniki Keklikoglou and Manolis Mandalakis and Eleftheria Fanouraki and Thekla I Anastasiou and Eirini Kagiampaki and Katerina Vasileiadou and Christina Pavloudi and Christos Arvanitidis and Michail Pavlidis},
url = {https://link.springer.com/10.1007/s43630-025-00744-5
/wp-content/uploads/2025/06/2025-Keklikoglou-PP-Sci-31.pdf},
doi = {10.1007/s43630-025-00744-5},
issn = {1474-905X, 1474-9092},
year = {2025},
date = {2025-06-20},
urldate = {2025-06-20},
journal = {Photochemical & Photobiological Sciences},
volume = {24},
number = {6},
pages = {1031--1040},
abstract = {Abstract

Anthropogenic light at night (ALAN) can have serious impacts on marine environments. Several studies have demonstrated that ALAN disrupts melatonin production, a hormone critical for regulating circadian rhythm. In this study, the effects of ALAN on melatonin and two of its related indolamines were investigated in the annelid
Hermodice carunculata
. Specifically, melatonin, serotonin and tryptamine levels were measured every three hours over a 24 h period in the heads of the annelids maintained under constant light and a 12 h light/12 h dark photoperiod, representing control conditions. Melatonin concentration was quantified using an enzyme immunoassay, while serotonin and tryptamine were analyzed by liquid chromatography–tandem mass spectrometry. Melatonin levels in annelid heads remained relatively constant with a pronounced peak at 11:00. A similar pattern was observed under constant light, but the melatonin peak shifted to 14:00. However, serotonin and tryptamine did not exhibit any significant diurnal variations due to constant light exposure. These findings suggest that melatonin secretion in
H. carunculata
is sensitive to ALAN, whereas its related indolamines are potentially not. The disruption of
H. carunculata'
s melatonin secretion pattern may affect its night-time behavior and reproduction, highlighting the need for further studies to assess the ecological effects of ALAN on various marine invertebrates.

Graphical abstract},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Oikonomou, Stavroula; Manousi, Domniki; Dimitroglou, Arkadios; Kazlari, Zoi; Loukovitis, Dimitrios; Papanna, Kantham; Tzokas, Konstantinos; Katribouzas, Nikos; Papaharisis, Leonidas; Tsigenopoulos, Costas S; Chatziplis, Dimitrios

Validation of QTL associated with resistance to Lernanthropus kroyeri in European seabass Journal Article

Aquaculture, 608 , pp. 742763, 2025, ISSN: 00448486.

Links | BibTeX

Rizzo, Lucia; Fernández, Tomás Vega; Necci, Francesca; Grelaud, Michaël; Ziveri, Patrizia; Gerovasileiou, Vasilis; Rossi, Sergio

Can Marine Animal Forests benefit from existing conservation measures? A systematic approach towards the identification of protected sessile benthic species in the Mediterranean Sea Journal Article

Mediterranean Marine Science, 26 (2), pp. 327–340, 2025, ISSN: 1791-6763, 1108-393X.

Abstract | Links | BibTeX

@article{rizzo_can_2025,
title = {Can Marine Animal Forests benefit from existing conservation measures? A systematic approach towards the identification of protected sessile benthic species in the Mediterranean Sea},
author = {Lucia Rizzo and Tomás Vega Fernández and Francesca Necci and Michaël Grelaud and Patrizia Ziveri and Vasilis Gerovasileiou and Sergio Rossi},
url = {https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/38550
/wp-content/uploads/2025/06/2025-Rizzo-MMS-29.pdf},
doi = {10.12681/mms.38550},
issn = {1791-6763, 1108-393X},
year = {2025},
date = {2025-06-17},
urldate = {2025-06-17},
journal = {Mediterranean Marine Science},
volume = {26},
number = {2},
pages = {327--340},
abstract = {Some marine benthic invertebrates increase the structural complexity of the seabed, thereby providing suitable habitats to several associated species, resulting in biodiversity hotspots. Marine Animal Forests (MAFs) encompass a set of the most important marine benthic habitats into which diversified sessile suspension feeders like sponges, corals, sea pens, tube worms, bivalves, bryozoans and ascidians occur. Such a mix of sessile species brings characteristic assemblages and supports important ecosystem functions. In the last decades, some species which form MAFs have been the object of international conventions, EU directives, and national policies aiming to address natural and human-induced disturbances. Effective conservation, monitoring, and restoration actions require summarizing the available information to include MAFs in conservation plans. In the present work, the main international policies for the protection of coastal and marine fauna were screened in order to provide a list of protected species which form Mediterranean MAFs. These international normative documents include the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES), the Convention on the Conservation of European Wildlife and Habitats (Bern Convention), the European Habitats Directive, and the Protocol concerning Specially Protected Areas and Biological Diversity in the Mediterranean (SPA/BD Protocol) of the Barcelona Convention. The analysis of the normative items revealed that 62 Mediterranean potential MAF forming species have been included in one or more legal annexes as protected species. These species belong to different phyla, including Porifera, Cnidaria, Bryozoa and Mollusca. A wider view was proposed by the International Union for Conservation of Nature (IUCN), setting the bases of a legal acknowledgement of the MAFs as one of the largest biomes on Earth. This study represents a first step to obtain a baseline of MAF legal protection framework in order to support the further implementation of management measures aimed at increasing the effective protection of MAFs. Reporting the status of the Mediterranean MAF species that should be considered in management plans and conservation measures will be crucial for policymakers, as well as for mitigating current and future impacts on these distinctive marine environments.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Some marine benthic invertebrates increase the structural complexity of the seabed, thereby providing suitable habitats to several associated species, resulting in biodiversity hotspots. Marine Animal Forests (MAFs) encompass a set of the most important marine benthic habitats into which diversified sessile suspension feeders like sponges, corals, sea pens, tube worms, bivalves, bryozoans and ascidians occur. Such a mix of sessile species brings characteristic assemblages and supports important ecosystem functions. In the last decades, some species which form MAFs have been the object of international conventions, EU directives, and national policies aiming to address natural and human-induced disturbances. Effective conservation, monitoring, and restoration actions require summarizing the available information to include MAFs in conservation plans. In the present work, the main international policies for the protection of coastal and marine fauna were screened in order to provide a list of protected species which form Mediterranean MAFs. These international normative documents include the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES), the Convention on the Conservation of European Wildlife and Habitats (Bern Convention), the European Habitats Directive, and the Protocol concerning Specially Protected Areas and Biological Diversity in the Mediterranean (SPA/BD Protocol) of the Barcelona Convention. The analysis of the normative items revealed that 62 Mediterranean potential MAF forming species have been included in one or more legal annexes as protected species. These species belong to different phyla, including Porifera, Cnidaria, Bryozoa and Mollusca. A wider view was proposed by the International Union for Conservation of Nature (IUCN), setting the bases of a legal acknowledgement of the MAFs as one of the largest biomes on Earth. This study represents a first step to obtain a baseline of MAF legal protection framework in order to support the further implementation of management measures aimed at increasing the effective protection of MAFs. Reporting the status of the Mediterranean MAF species that should be considered in management plans and conservation measures will be crucial for policymakers, as well as for mitigating current and future impacts on these distinctive marine environments.

Kouvara, Konstantina; Lazou-Laskaridis, Evi; Xirotagarou, Peggy; Christodoulou, Dimitris; Dimas, Xenophon; Geraga, Maria; Giovos, Ioannis; Charitou, Anastasia; Gerovasileiou, Vasilis; Galgani, Francois; Papatheodorou, George

Assessing marine litter and its ecological impact on the seafloor of Thermaikos Gulf (NE Mediterranean Sea, Greece): Insights from ROV and diver surveys Journal Article

Marine Pollution Bulletin, 217 , pp. 118109, 2025, ISSN: 0025326X.

Links | BibTeX

Asimaki, Adamantia; Psofakis, Pier; Gkalogianni, Elli-Zafeiria; Katouni, Aikaterini-Maria; Berillis, Panagiotis; Kormas, Konstantinos A; Rumbos, Christos I; Athanassiou, Christos G; Vasilaki, Antigoni; Fountoulaki, Eleni; Henry, Morgane; Mente, Eleni; Gisbert, Enric; Karapanagiotidis, Ioannis T

The Effects of Dietary Fishmeal Substitution by Full‐Fat and Defatted textitZophobas morio Larvae Meals on Juvenile Gilthead Seabream (Sparus aurata): An Integrative Approach Journal Article

Aquaculture Nutrition, 2025 (1), pp. 8885509, 2025, ISSN: 1353-5773, 1365-2095.

Abstract | Links | BibTeX

@article{asimaki_effects_2025,
title = {The Effects of Dietary Fishmeal Substitution by Full‐Fat and Defatted textitZophobas morio Larvae Meals on Juvenile Gilthead Seabream (\textit{Sparus aurata}): An Integrative Approach},
author = {Adamantia Asimaki and Pier Psofakis and Elli-Zafeiria Gkalogianni and Aikaterini-Maria Katouni and Panagiotis Berillis and Konstantinos A Kormas and Christos I Rumbos and Christos G Athanassiou and Antigoni Vasilaki and Eleni Fountoulaki and Morgane Henry and Eleni Mente and Enric Gisbert and Ioannis T Karapanagiotidis},
editor = {Lee Seong Wei},
url = {https://onlinelibrary.wiley.com/doi/10.1155/anu/8885509
/wp-content/uploads/2025/06/2025-Asimaki-Aq-Nutr-27.pdf},
doi = {10.1155/anu/8885509},
issn = {1353-5773, 1365-2095},
year = {2025},
date = {2025-06-10},
urldate = {2025-06-10},
journal = {Aquaculture Nutrition},
volume = {2025},
number = {1},
pages = {8885509},
abstract = {This study evaluated the dietary fishmeal substitution by full‐fat (FF) and defatted (DF)
Zophobas morio
meals regarding growth, feed efficiency, proximate and fatty acid compositions, digestive enzymes activities, histology and midgut microbiota in gilthead seabream (
Sparus aurata
). Juveniles initially weighing 3.4 g were distributed to triplicate groups and fed at satiation six isonitrogenous (8.41%) and isocaloric (21 Mj/kg) diets for 100 days. An insect meal‐free diet was the control (CTRL), two diets contained a FF
Z. morio
meal at 49 g/kg (FF‐49) and 97 g/kg (FF‐97), and three diets contained a DF
Z. morio
meal at 58 g/kg (DF‐58), 116 g/kg (DF‐116) and 174 g/kg (DF‐174) at the expense of fishmeal. Neither the form nor the inclusion level of
Z. morio
meals affected the feed intake of fish denoting a similar acceptability to that of fishmeal. Fish survival, growth and feed efficiency were not impaired by all dietary inclusion levels of
Z. morio
meals. Proximate composition of fish was altered but without indicating a clear correlation with the form or inclusion level of
Z. morio
. Increasing inclusions of both forms of
Z. morio
meals tended to decrease 22:6n‐3, 20:5n‐3, 18:3n‐3 and 18:2n‐6 levels in fish tissues. All fish exhibited similar proteolytic enzyme activities, but the increasing inclusions of both insect meal forms led to gradual increases in the lipase and α‐amylase activities indicating a compensatory mechanism for lipid and carbohydrate digestion. The use of
Z. morio
meals led to some mild histomorphological changes in the intestine and liver that were more pronounced in fish fed the FF form at the highest inclusion level. Midgut bacterial communities of the groups were similar and dominated by potentially beneficial members of
Saccharimonadales
and
Rhodobacteraceae
, except FF‐97 fish that had high abundances of
Legionella
‐ and
Pandoraea
‐like bacteria. To conclude,
Z. morio
meal, either FF or DF, is a suitable insect protein for fishmeal substitution towards more sustainable aquafeeds for
S. aurata
.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

This study evaluated the dietary fishmeal substitution by full‐fat (FF) and defatted (DF)
Zophobas morio
meals regarding growth, feed efficiency, proximate and fatty acid compositions, digestive enzymes activities, histology and midgut microbiota in gilthead seabream (
Sparus aurata
). Juveniles initially weighing 3.4 g were distributed to triplicate groups and fed at satiation six isonitrogenous (8.41%) and isocaloric (21 Mj/kg) diets for 100 days. An insect meal‐free diet was the control (CTRL), two diets contained a FF
Z. morio
meal at 49 g/kg (FF‐49) and 97 g/kg (FF‐97), and three diets contained a DF
Z. morio
meal at 58 g/kg (DF‐58), 116 g/kg (DF‐116) and 174 g/kg (DF‐174) at the expense of fishmeal. Neither the form nor the inclusion level of
Z. morio
meals affected the feed intake of fish denoting a similar acceptability to that of fishmeal. Fish survival, growth and feed efficiency were not impaired by all dietary inclusion levels of
Z. morio
meals. Proximate composition of fish was altered but without indicating a clear correlation with the form or inclusion level of
Z. morio
. Increasing inclusions of both forms of
Z. morio
meals tended to decrease 22:6n‐3, 20:5n‐3, 18:3n‐3 and 18:2n‐6 levels in fish tissues. All fish exhibited similar proteolytic enzyme activities, but the increasing inclusions of both insect meal forms led to gradual increases in the lipase and α‐amylase activities indicating a compensatory mechanism for lipid and carbohydrate digestion. The use of
Z. morio
meals led to some mild histomorphological changes in the intestine and liver that were more pronounced in fish fed the FF form at the highest inclusion level. Midgut bacterial communities of the groups were similar and dominated by potentially beneficial members of
Saccharimonadales
and
Rhodobacteraceae
, except FF‐97 fish that had high abundances of
Legionella
‐ and
Pandoraea
‐like bacteria. To conclude,
Z. morio
meal, either FF or DF, is a suitable insect protein for fishmeal substitution towards more sustainable aquafeeds for
S. aurata
.

Okeleye, Oluwabusayo Israel; Majstorovic, Jovana; Sudharaka, Anupa; Triga, Adriana; Mandalakis, Manolis; Katharios, Pantelis; Mladineo, Ivona

Common carp (Cyprinus carpio) blood cells are modulated by a parasitic antimicrobial peptide, A-2S and Edwardsiella spp. strains Journal Article

Fish & Shellfish Immunology, 164 , pp. 110427, 2025, ISSN: 10504648.

Links | BibTeX

Cascarano, Maria Chiara; Sevasti, Papadogiorgaki; Katharios, Pantelis

Ultrastructural Insights Into a textitCandidatus Parilichlamydia sp. Infection of Gill Goblet Cells in Greater Amberjack Journal Article

Journal of Fish Diseases, pp. e14146, 2025, ISSN: 0140-7775, 1365-2761.

Abstract | Links | BibTeX

Siaperas, Romanos; Taxeidis, George; Gioti, Anastasia; Nikolaivits, Efstratios; Topakas, Evangelos

Multi-omics insights into the response of Aspergillus parasiticus to long-chain alkanes in relation to polyethylene modification Journal Article

Environmental Pollution, 376 , pp. 126386, 2025, ISSN: 02697491.

Links | BibTeX

Díaz-Avello, Ulises G; Skouridou, Vasso; Shkembi, Xhensila; Reverté, Jaume; Mandalakis, Manolis; Peristeraki, Panagiota; Campàs, Mònica; O'Sullivan, Ciara K

Aptamer-antibody sandwich lateral flow test for rapid visual detection of tetrodotoxin in pufferfish Journal Article

Science of The Total Environment, 978 , pp. 179419, 2025, ISSN: 00489697.

Links | BibTeX

Ventriglia, Gianluca; Duncan, Neil; Giménez, Ignacio; Mylonas, Constantinos C; Pousis, Chrysovalentinos; Corriero, Aldo; Zupa, Rosa

Spermatogenesis advancement in pre-pubertal meagre Argyrosomus regius treated with recombinant gonadotropins Journal Article

Scientific Reports, 15 (1), pp. 15113, 2025, ISSN: 2045-2322.

Links | BibTeX

Papadaki, Maria; Le, Ngoc-Son; Mylonas, Constantinos C; Sarropoulou, Elena

Exploring the Fanconi Anemia Gene Expression and Regulation by MicroRNAs in Gilthead Seabream (Sparus aurata) at Different Gonadal Development Stages Journal Article

Marine Biotechnology, 27 (2), pp. 74, 2025, ISSN: 1436-2228, 1436-2236.

Abstract | Links | BibTeX

@article{papadaki_exploring_2025,
title = {Exploring the Fanconi Anemia Gene Expression and Regulation by MicroRNAs in Gilthead Seabream (Sparus aurata) at Different Gonadal Development Stages},
author = {Maria Papadaki and Ngoc-Son Le and Constantinos C Mylonas and Elena Sarropoulou},
url = {https://link.springer.com/10.1007/s10126-025-10444-x
/wp-content/uploads/2025/05/2025-Papadaki-MarBioteck-21.pdf},
doi = {10.1007/s10126-025-10444-x},
issn = {1436-2228, 1436-2236},
year = {2025},
date = {2025-05-06},
urldate = {2025-05-06},
journal = {Marine Biotechnology},
volume = {27},
number = {2},
pages = {74},
abstract = {Abstract

Fanconi anaemia (FA) is a rare autosomal recessive disease in humans that is distributed worldwide. Fanconi anemia complementation (Fanc) proteins are essential for the appropriate functioning of the FA DNA repair pathway. They are also linked to a number of other biological processes, including oxygen metabolism, cell cycle regulation, haematopoiesis and apoptosis. So far, little research has been conducted on teleosts, but evidence shows that Fanc proteins play a significant role in immune response and sex reversal. For the examination of the expression of three
fanc
genes (
fancc
,
fancl,
and
fancd2
), as well as the potential regulation of these genes by microRNAs (miRNAs) in gonadal tissues at different stages of development, the present study has selected the gilthead seabream (
Sparus aurata
), a significant aquaculture species that exhibits protandrous hermaphroditism. The obtained data suggested the role of
fancl
and
fancd2
in the maturation of female gonads and the miRNAs miR-210, miR-217 and miR-10926 have been identified as putative regulators of
fancd2
,
fancc
and
fancl
, respectively. Overall, the data indicated the potential use of
fancl
and
fancd2
genes as sex biomarkers in conjunction with their respective regulation by miRNAs. To the best of our knowledge, this is the first study demonstrating the importance of
fanc
genes, along with putative regulatory miRNAs, in the reproduction of an important marine aquaculture species.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Thieme, Philipp; Fischbach, Vivian; Papadakis, Ioannis; Moritz, Timo

Development of convergent adaptations reveal highly conserved early ontogenetic skull shape in fishes with amphibious vision Journal Article

Scientific Reports, 15 (1), pp. 8411, 2025, ISSN: 2045-2322.

Links | BibTeX

Kapelonis, Zacharias; Chatzigeorgiou, Georgios; Ntoumas, Manolis; Grigoriou, Panos; Pettas, Manos; Michelinakis, Spyros; Correia, Ricardo; Lemos, Catarina Rasquilha; Pinheiro, Luis Menezes; Lomba, Caio; Fortuna, João; Loureiro, Rui; Santos, André; Chatzinikolaou, Eva

Flying Robots Teach Floating Robots—A Machine Learning Approach for Marine Habitat Mapping Based on Combined Datasets Journal Article

Journal of Marine Science and Engineering, 13 (3), pp. 611, 2025, ISSN: 2077-1312.

Abstract | Links | BibTeX

@article{kapelonis_flying_2025,
title = {Flying Robots Teach Floating Robots—A Machine Learning Approach for Marine Habitat Mapping Based on Combined Datasets},
author = {Zacharias Kapelonis and Georgios Chatzigeorgiou and Manolis Ntoumas and Panos Grigoriou and Manos Pettas and Spyros Michelinakis and Ricardo Correia and Catarina Rasquilha Lemos and Luis Menezes Pinheiro and Caio Lomba and João Fortuna and Rui Loureiro and André Santos and Eva Chatzinikolaou},
url = {https://www.mdpi.com/2077-1312/13/3/611
/wp-content/uploads/2025/04/2025-Kapelonis-Mar-Sci-Eng-19.pdf},
doi = {10.3390/jmse13030611},
issn = {2077-1312},
year = {2025},
date = {2025-04-11},
urldate = {2025-04-11},
journal = {Journal of Marine Science and Engineering},
volume = {13},
number = {3},
pages = {611},
abstract = {Unmanned aerial and autonomous surface vehicles (UAVs and ASVs, respectively) are two emerging technologies for the mapping of coastal and marine environments. Using UAV photogrammetry, the sea-bottom composition can be resolved with very high fidelity in shallow waters. At greater depths, acoustic methodologies have far better propagation properties compared to optics; therefore, ASVs equipped with multibeam echosounders (MBES) are better-suited for mapping applications in deeper waters. In this work, a sea-bottom classification methodology is presented for mapping the protected habitat of Mediterranean seagrass Posidonia oceanica (habitat code 1120) in a coastal subregion of Heraklion (Crete, Greece). The methodology implements a machine learning scheme, where knowledge obtained from UAV imagery is embedded (through training) into a classifier that utilizes acoustic backscatter intensity and features derived from the MBES data provided by an ASV. Accuracy and precision scores of greater than 85% compared with visual census ground-truth data for both optical and acoustic classifiers indicate that this hybrid mapping approach is promising to mitigate the depth-induced bias in UAV-only models. The latter is especially interesting in cases where the studied habitat boundaries extend beyond depths that can be studied via aerial devices’ optics, as is the case with P. oceanica meadows.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Mukiibi, Robert; Ferraresso, Serena; Franch, Rafaella; Peruzza, Luca; Rovere, Giulia Dalla; Babbucci, Massimiliano; Bertotto, Daniela; Toffan, Anna; Pascoli, Francesco; Faggion, Sara; Peñaloza, Carolina; Tsigenopoulos, Costas S; Houston, Ross D; Bargelloni, Luca; Robledo, Diego

Integrated functional genomic analysis identifies regulatory variants underlying a major QTL for disease resistance in European sea bass Journal Article

BMC Biology, 23 (1), pp. 75, 2025, ISSN: 1741-7007.

Abstract | Links | BibTeX

@article{mukiibi_integrated_2025,
title = {Integrated functional genomic analysis identifies regulatory variants underlying a major QTL for disease resistance in European sea bass},
author = {Robert Mukiibi and Serena Ferraresso and Rafaella Franch and Luca Peruzza and Giulia Dalla Rovere and Massimiliano Babbucci and Daniela Bertotto and Anna Toffan and Francesco Pascoli and Sara Faggion and Carolina Peñaloza and Costas S Tsigenopoulos and Ross D Houston and Luca Bargelloni and Diego Robledo},
url = {https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-025-02180-4
/wp-content/uploads/2025/04/2025-Mukiibi-BMC-Biology-18.pdf},
doi = {10.1186/s12915-025-02180-4},
issn = {1741-7007},
year = {2025},
date = {2025-04-07},
urldate = {2025-04-07},
journal = {BMC Biology},
volume = {23},
number = {1},
pages = {75},
abstract = {Abstract

Background
Viral nervous necrosis (VNN) is an important viral disease threatening global aquaculture sustainability and affecting over 50 farmed and ecologically important fish species. A major QTL for resistance to VNN has been previously detected in European sea bass, but the underlying causal gene(s) and mutation(s) remain unknown. To identify the mechanisms and genetic factors underpinning resistance to VNN in European sea bass, we employed integrative analyses of multiple functional genomics assays in European sea bass.

Results

The estimated heritability of VNN resistance was high (
h
2
textasciitilde 0.40), and a major QTL explaining up to 38% of the genetic variance of the trait was confirmed on chromosome 3, with individuals with the resistant QTL genotype showing a 90% survivability against a VNN outbreak. Whole-genome resequencing analyses narrowed the location of this QTL to a small region containing 4 copies of interferon alpha inducible protein 27-like 2A (
IFI27L2A
) genes, and one copy of the interferon alpha inducible protein 27-like 2 (
IFI27L2
) gene. RNA sequencing revealed a clear association between the QTL genotype and the expression of two of the
IFI27L2A
genes, and the
IFI27L2
gene. Integration with chromatin accessibility and histone modification data pinpointed two SNPs in active regulatory regions of two of these genes (
IFI27L2A
and
IFI27L2
), and transcription factor binding site gains for the resistant alleles were predicted. These alleles, particularly the SNP variant CHR3:10,077,301, exhibited higher frequencies (0.55 to 0.77) in Eastern Mediterranean Sea bass populations, which show considerably higher levels of resistance to VNN, as compared to susceptible West Mediterranean and Atlantic populations (0.15–0.25).

Conclusions

The SNP variant CHR3:10,077,301, through modulation of
IFI27L2
and
IFI27L2A
genes, is likely the causative mutation underlying resistance to VNN in European sea bass. This is one of the first causative mutations discovered for disease resistance traits in fish and paves the way for marker-assisted selection as well as biotechnological approaches to enhance resistance to VNN in European sea bass and other susceptible species.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Abstract

Background
Viral nervous necrosis (VNN) is an important viral disease threatening global aquaculture sustainability and affecting over 50 farmed and ecologically important fish species. A major QTL for resistance to VNN has been previously detected in European sea bass, but the underlying causal gene(s) and mutation(s) remain unknown. To identify the mechanisms and genetic factors underpinning resistance to VNN in European sea bass, we employed integrative analyses of multiple functional genomics assays in European sea bass.

Results

The estimated heritability of VNN resistance was high (
h
2
textasciitilde 0.40), and a major QTL explaining up to 38% of the genetic variance of the trait was confirmed on chromosome 3, with individuals with the resistant QTL genotype showing a 90% survivability against a VNN outbreak. Whole-genome resequencing analyses narrowed the location of this QTL to a small region containing 4 copies of interferon alpha inducible protein 27-like 2A (
IFI27L2A
) genes, and one copy of the interferon alpha inducible protein 27-like 2 (
IFI27L2
) gene. RNA sequencing revealed a clear association between the QTL genotype and the expression of two of the
IFI27L2A
genes, and the
IFI27L2
gene. Integration with chromatin accessibility and histone modification data pinpointed two SNPs in active regulatory regions of two of these genes (
IFI27L2A
and
IFI27L2
), and transcription factor binding site gains for the resistant alleles were predicted. These alleles, particularly the SNP variant CHR3:10,077,301, exhibited higher frequencies (0.55 to 0.77) in Eastern Mediterranean Sea bass populations, which show considerably higher levels of resistance to VNN, as compared to susceptible West Mediterranean and Atlantic populations (0.15–0.25).

Conclusions

The SNP variant CHR3:10,077,301, through modulation of
IFI27L2
and
IFI27L2A
genes, is likely the causative mutation underlying resistance to VNN in European sea bass. This is one of the first causative mutations discovered for disease resistance traits in fish and paves the way for marker-assisted selection as well as biotechnological approaches to enhance resistance to VNN in European sea bass and other susceptible species.

Wei, Xiao-Lei; Hao, Zhi-Wei; Kotzamanis, Yannis P; Zhang, Tian-Hua; Liu, Zhi-Bo; Yang, Hong; Luo, Zhi

Dietary iron oxide (Fe2O3) nanoparticles modulate growth performance, body composition, mineral content and intestinal health of yellow catfish juveniles (Pelteobagrus fulvidraco) Journal Article

Aquaculture Reports, 42 , pp. 102739, 2025, ISSN: 23525134.

Links | BibTeX

Antoniou, Theodora; Dimitriou, Andreas C; Karameta, Emmanouela; Antoniou, Aglaia; Poulakakis, Nikos; Sfenthourakis, Spyros

Comparative phylogeography of four lizard taxa within an oceanic island Journal Article

Molecular Phylogenetics and Evolution, 205 , pp. 108295, 2025, ISSN: 10557903.

Links | BibTeX

Droubogiannis, Stavros; Katharios, Pantelis

Phage Therapy in Aquaculture Incollection

Elumalai, Preetham; Lakshmi, Sreeja (Ed.): Antimicrobial Resistance in Aquaculture and Aquatic Environments, pp. 229–255, Springer Nature Singapore, Singapore, 2025, ISBN: 9789819773190 9789819773206.

Links | BibTeX

Chalmoukis, Charalampos; Droubogiannis, Stavros; Michalopoulou, Vassiliki A; Triga, Adriana; Sarris, Panagiotis F; Katharios, Pantelis

Development and Characterization of Fluorescent Protein-Tagged Vibrio harveyi Strains as a Versatile Tool for Studying Infection Dynamics and Strain Interactions Journal Article

Pathogens, 14 (3), pp. 247, 2025, ISSN: 2076-0817.

Abstract | Links | BibTeX

@article{chalmoukis_development_2025,
title = {Development and Characterization of Fluorescent Protein-Tagged Vibrio harveyi Strains as a Versatile Tool for Studying Infection Dynamics and Strain Interactions},
author = {Charalampos Chalmoukis and Stavros Droubogiannis and Vassiliki A Michalopoulou and Adriana Triga and Panagiotis F Sarris and Pantelis Katharios},
url = {https://www.mdpi.com/2076-0817/14/3/247
/wp-content/uploads/2025/03/2025-Chalmoukis-pathogens-13.pdf},
doi = {10.3390/pathogens14030247},
issn = {2076-0817},
year = {2025},
date = {2025-03-18},
urldate = {2025-03-18},
journal = {Pathogens},
volume = {14},
number = {3},
pages = {247},
abstract = {Fluorescent protein-tagged bacterial strains are widely used tools for studying host-pathogen interactions and microbial dynamics. In this study, we developed and characterized Vibrio harveyi strains genetically modified to express green fluorescent protein (GFP) and red fluorescent protein (RFP). These strains were constructed using triparental mating and evaluated for phenotypic, genomic, and virulence attributes. Genomic analyses revealed strain-specific variations, including mutations in key regulatory and metabolic genes, such as luxO and transketolase. While plasmid acquisition imposed metabolic costs, resulting in altered growth and antibiotic sensitivities in certain transconjugants, others demonstrated robust phenotypic stability. Virulence assays using gilthead seabream larvae revealed that most tagged strains retained moderate pathogenicity, with visualization of co-infections highlighting the potential for studying strain-specific interactions. Furthermore, fluorescent microscopy confirmed the successful colonization and localization of tagged bacteria within host tissues. These findings underscore the utility of GFP- and RFP-tagged Vibrio harveyi as versatile tools for infection dynamics, offering a foundation for future research on strain interactions and pathogen-host relationships.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Costanzo, Di F; Marsico, Di M; Orefice, I; Kristoffersen, J B; Kasapidis, P; Chaumier, T; Ambrosino, L; Miralto, M; Cigliano, Aiese R; Verret, F; Tirichine, L; Trindade, M; Zyl, Van L; Dato, Di V; Romano, G

High-quality genome assembly and annotation of Thalassiosira rotula (synonym of Thalassiosira gravida) Journal Article

Scientific Data, 12 (1), pp. 310, 2025, ISSN: 2052-4463.

Links | BibTeX

Sarropoulou, Elena; Katharios, Pantelis; Kaitetzidou, Elisavet; Scapigliati, Giuseppe; Miccoli, Andrea

Circulating miRNAs involved in the immune response of the European seabass (Dicentrarchus labrax) Journal Article

Fish & Shellfish Immunology, 160 , pp. 110232, 2025, ISSN: 10504648.

Links | BibTeX

Katharios, Pantelis; Karageorgou, Evangelia

Protozoan Co‐Infection Drives Fish Mortality Event in Crete's Karteros River Journal Article

Journal of Fish Diseases, pp. e14099, 2025, ISSN: 0140-7775, 1365-2761.

Abstract | Links | BibTeX

Brecko, Jonathan; Mathys, Aurore; Chatzinikolaou, Eva; Keklikoglou, Kleoniki; Blettery, Jonathan; Green, Laura; Musson, Alicia; Paton, Alan; Phillips, Sarah; Bastir, Markus; Wiltschke, Karin; Rainer, Heimo; Kroh, Andreas; Haston, Elspeth; Semal, Patrick

DIGIT-KEY: an aid towards uniform 2D+ and 3D digitisation techniques within natural history collections Journal Article

European Journal of Taxonomy, 976 , 2025, ISSN: 2118-9773.

Abstract | Links | BibTeX

@article{brecko_digit-key_2025,
title = {DIGIT-KEY: an aid towards uniform 2D+ and 3D digitisation techniques within natural history collections},
author = {Jonathan Brecko and Aurore Mathys and Eva Chatzinikolaou and Kleoniki Keklikoglou and Jonathan Blettery and Laura Green and Alicia Musson and Alan Paton and Sarah Phillips and Markus Bastir and Karin Wiltschke and Heimo Rainer and Andreas Kroh and Elspeth Haston and Patrick Semal},
url = {https://europeanjournaloftaxonomy.eu/index.php/ejt/article/view/2797
/wp-content/uploads/2025/03/2025-Brecko-EuJTax-9.pdf},
doi = {10.5852/ejt.2025.976.2797},
issn = {2118-9773},
year = {2025},
date = {2025-03-04},
urldate = {2025-03-04},
journal = {European Journal of Taxonomy},
volume = {976},
abstract = {Natural History institutes hold an immense number of specimens and artefacts. For years these collections were not accessible online, remaining inaccessible to researchers from far away and hidden from the general public. Large digitisation projects and cross-institutional agreements aim to bring their collections into the digital era, such as the SYNTHESYS+ project and the Distributed System of Scientific Collections (DiSSCo) Research Infrastructure. As specimens are 3D physical objects with different characteristics many techniques are available to 3D digitise them. For inexperienced users this can be quite overwhelming. Which techniques are already well tested in other institutions and are suitable for a specific specimen or collection? To investigate this, we have set up a dichotomous identification key for digitisation techniques: DIGIT-KEY, (https://digit.naturalheritage.be/digit-key). For each technique, examples used in SYNTHESYS+ Institutions are visualised and training manuals provided. All information can be easily updated and representatives can be contacted if necessary to request more information about a certain technique. This key can be helpful to achieve comparable results across institutions when digitising collections on demand in future DiSSCo research initiatives coordinated through the European Loans and Visits System (ELViS) for Virtual and Transnational Access.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Grigorakis, Kriton; Kogiannou, Dimitra; Kotsiri, Mado; Kleidas, Ioannis; Mello, Paulo De H; Habiballah, Salaheldeen; Alshaikhi, Ali; Alhafedh, Youssef S; Mohamed, Asaad H W

Freshness and Spoilage Patterns of Wild and Farmed Tropical Fish Species with Major Commercial Importance Originating from Saudi Arabian Waters Journal Article

Foods, 14 (4), pp. 690, 2025, ISSN: 2304-8158.

Abstract | Links | BibTeX

Carlot, J; Galobart, C; Gómez-Gras, D; Santamaría, J; Golo, R; Sini, M; Cebrian, E; Gerovasileiou, V; Ponti, M; Turicchia, E; Comeau, S; Rilov, G; Tamburello, L; Mantas, Pulido T; Cerrano, C; Ledoux, J B; Gattuso, J -P; Ramirez-Calero, S; Millan, L; Montefalcone, M; Katsanevakis, S; Bensoussan, N; Garrabou, J; Teixidó, N

Vulnerability of benthic trait diversity across the Mediterranean Sea following mass mortality events Journal Article

Nature Communications, 16 (1), pp. 1571, 2025, ISSN: 2041-1723.

Abstract | Links | BibTeX

@article{carlot_vulnerability_2025,
title = {Vulnerability of benthic trait diversity across the Mediterranean Sea following mass mortality events},
author = {J Carlot and C Galobart and D Gómez-Gras and J Santamaría and R Golo and M Sini and E Cebrian and V Gerovasileiou and M Ponti and E Turicchia and S Comeau and G Rilov and L Tamburello and T Pulido Mantas and C Cerrano and J B Ledoux and J -P Gattuso and S Ramirez-Calero and L Millan and M Montefalcone and S Katsanevakis and N Bensoussan and J Garrabou and N Teixidó},
url = {https://www.nature.com/articles/s41467-025-55949-0
/wp-content/uploads/2025/02/2025-Carlot-Nat-Comm-7.pdf},
doi = {10.1038/s41467-025-55949-0},
issn = {2041-1723},
year = {2025},
date = {2025-02-21},
urldate = {2025-02-20},
journal = {Nature Communications},
volume = {16},
number = {1},
pages = {1571},
abstract = {Abstract
Unraveling the functional future of marine ecosystems amid global change poses a pressing challenge. This is particularly critical in the Mediterranean Sea, which is highly impacted by global and local drivers. Utilizing extensive mass mortality events (MMEs) datasets spanning from 1986 to 2020 across the Mediterranean Sea, we investigated the trait vulnerability of benthic species that suffered from MMEs induced by nine distinct mortality drivers. By analyzing changes in ten ecological traits across 389 benthic species—constituting an extensive compendium of Mediterranean ecological traits to date—we identified 228 functional entities (FEs), defined as groups of species sharing the same trait values. Our findings indicate that of these 55 FEs were impacted by MMEs, accentuating a heightened vulnerability within specific trait categories. Notably, more than half of the mortality records showed severe impacts on calcifying and larger species with slower growth which mostly account for tree-like and massive forms. Altogether, we highlight that 29 FEs suffered extreme mortality, leading to a maximum increase of 19.1% of the global trait volume vulnerability over 35 years. We also reveal that 10.8% of the trait volume may have been temporarily lost over the last five years, emphasizing the risk of a rapid ecological transformation in the Mediterranean Sea.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Daraghmeh, Nauras; Exter, Katrina; Pagnier, Justine; Balazy, Piotr; Cancio, Ibon; Chatzigeorgiou, Giorgos; Chatzinikolaou, Eva; Chelchowski, Maciej; Chrismas, Nathan Alexis Mitchell; Comtet, Thierry; Dailianis, Thanos; Deneudt, Klaas; Cerio, Oihane Diaz De; Digenis, Markos; Gerovasileiou, Vasilis; González, José; Kauppi, Laura; Kristoffersen, Jon Bent; Kukliński, Piotr; Lasota, Rafał; Levy, Liraz; Małachowicz, Magdalena; Mavrič, Borut; Mortelmans, Jonas; Paredes, Estefania; Poćwierz‐Kotus, Anita; Reiss, Henning; Santi, Ioulia; Sarafidou, Georgia; Skouradakis, Grigorios; Solbakken, Jostein; Staehr, Peter A U; Tajadura, Javier; Thyrring, Jakob; Troncoso, Jesus S; Vernadou, Emmanouela; Viard, Frederique; Zafeiropoulos, Haris; Zbawicka, Małgorzata; Pavloudi, Christina; Obst, Matthias

A Long-Term Ecological Research Data Set From the Marine Genetic Monitoring Program ARMS-MBON 2018–2020 Journal Article

Molecular Ecology Resources, pp. e14073, 2025, ISSN: 1755-098X, 1755-0998.

Abstract | Links | BibTeX

@article{daraghmeh_longterm_2025,
title = {A Long-Term Ecological Research Data Set From the Marine Genetic Monitoring Program ARMS-MBON 2018–2020},
author = {Nauras Daraghmeh and Katrina Exter and Justine Pagnier and Piotr Balazy and Ibon Cancio and Giorgos Chatzigeorgiou and Eva Chatzinikolaou and Maciej Chelchowski and Nathan Alexis Mitchell Chrismas and Thierry Comtet and Thanos Dailianis and Klaas Deneudt and Oihane Diaz De Cerio and Markos Digenis and Vasilis Gerovasileiou and José González and Laura Kauppi and Jon Bent Kristoffersen and Piotr Kukliński and Rafał Lasota and Liraz Levy and Magdalena Małachowicz and Borut Mavrič and Jonas Mortelmans and Estefania Paredes and Anita Poćwierz‐Kotus and Henning Reiss and Ioulia Santi and Georgia Sarafidou and Grigorios Skouradakis and Jostein Solbakken and Peter A U Staehr and Javier Tajadura and Jakob Thyrring and Jesus S Troncoso and Emmanouela Vernadou and Frederique Viard and Haris Zafeiropoulos and Małgorzata Zbawicka and Christina Pavloudi and Matthias Obst},
url = {https://onlinelibrary.wiley.com/doi/10.1111/1755-0998.14073
/wp-content/uploads/2025/02/2025-Daraghmeh-MolEcoRes-6.pdf},
doi = {10.1111/1755-0998.14073},
issn = {1755-098X, 1755-0998},
year = {2025},
date = {2025-02-05},
urldate = {2025-02-05},
journal = {Molecular Ecology Resources},
pages = {e14073},
abstract = {ABSTRACT

Molecular methods such as DNA/eDNA metabarcoding have emerged as useful tools to document the biodiversity of complex communities over large spatio‐temporal scales. We established an international Marine Biodiversity Observation Network (ARMS‐MBON) combining standardised sampling using autonomous reef monitoring structures (ARMS) with metabarcoding for genetic monitoring of marine hard‐bottom benthic communities. Here, we present the data of our first sampling campaign comprising 56 ARMS units deployed in 2018–2019 and retrieved in 2018–2020 across 15 observatories along the coasts of Europe and adjacent regions. We describe the open‐access data set (image, genetic and metadata) and explore the genetic data to show its potential for marine biodiversity monitoring and ecological research. Our analysis shows that ARMS recovered more than 60 eukaryotic phyla capturing diversity of up to textasciitilde5500 amplicon sequence variants and textasciitilde1800 operational taxonomic units, and up to textasciitilde250 and textasciitilde50 species per observatory using the cytochrome
c
oxidase subunit I (COI) and 18S rRNA marker genes, respectively. Further, ARMS detected threatened, vulnerable and non‐indigenous species often targeted in biological monitoring. We show that while deployment duration does not drive diversity estimates, sampling effort and sequencing depth across observatories do. We recommend that ARMS should be deployed for at least 3–6 months during the main growth season to use resources as efficiently as possible and that post‐sequencing curation is applied to enable statistical comparison of spatio‐temporal entities. We suggest that ARMS should be used in biological monitoring programs and long‐term ecological research and encourage the adoption of our ARMS‐MBON protocols.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Chen, I-Hao; Georgopoulou, Dimitra G; Ebbesson, Lars O E; Voskakis, Dimitris; Munthe-Kaas, Antonella Zanna; Papandroulakis, Nikos

Acoustic tags versus camera—a case study on feeding behaviour of European seabass in sea cages Journal Article

Frontiers in Marine Science, 11 , pp. 1497336, 2025, ISSN: 2296-7745.

Abstract | Links | BibTeX

@article{chen_acoustic_2025,
title = {Acoustic tags versus camera—a case study on feeding behaviour of European seabass in sea cages},
author = {I-Hao Chen and Dimitra G Georgopoulou and Lars O E Ebbesson and Dimitris Voskakis and Antonella Zanna Munthe-Kaas and Nikos Papandroulakis},
url = {https://www.frontiersin.org/articles/10.3389/fmars.2024.1497336/full
https://imbbc.hcmr.gr/wp-content/uploads/2025/01/2025-Chen-fmars-5.pdf},
doi = {10.3389/fmars.2024.1497336},
issn = {2296-7745},
year = {2025},
date = {2025-01-30},
urldate = {2025-01-30},
journal = {Frontiers in Marine Science},
volume = {11},
pages = {1497336},
abstract = {Intoduction
With the expansion of the aquaculture industry, the need arises for scalable, reliable, and robust methods to assess fish behaviour in sea cages to guide operational management, which includes feeding optimisation and welfare assessments. Fish cage monitoring utilising either acoustic transmitters or underwater cameras is well-studied. However, the relationship between those two different measurement types seems to have not been explored, nor have they been evaluated together in one experimental site.

Methods

In our 1-month study, we compared the activity of 14 sentinel fish and the artificial intelligence (AI)-inferred speed of individuals from the European seabass (
Dicentrarchus labrax
) sea cage population in three feeding trials. Comparisons include a maximum activity comparison using persistent peaks, fish behavioural pattern establishment and retention, and periodical behavioural patterns.

Results
Our results demonstrate that under certain circumstances, both technologies are interchangeable from the perspective of persistent peaks and periodicity, but complementary when it comes to behaviour analysis such as food anticipatory behaviour (FAB).

Discussion
We anticipate that our findings will stimulate advances where multiple sensor types are in use to achieve a more holistic understanding of fish behaviour in the aquaculture sector using underwater technologies.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Oikonomou, Stavroula; Tasiouli, Katerina; Tsaparis, Dimitrios; Manousaki, Tereza; Vallecillos, Antonio; Oikonomaki, Katerina; Tzokas, Konstantinos; Katribouzas, Nikolaos; Batargias, Costas; Chatziplis, Dimitrios; Tsigenopoulos, Costas S

Genomic evaluation for body weight, length and growth estimates in meagre Argyrosomus regius Journal Article

Aquaculture, 595 , pp. 741622, 2025, ISSN: 00448486.

Links | BibTeX

Lancerotto, Stefano; Fakriadis, Ioannis; Papadaki, Maria; Giménez, Ignacio; Genovés, José Vicente Roig; Mylonas, Constantinos C

Aquaculture, 594 , pp. 741401, 2025, ISSN: 00448486.

Links | BibTeX

Dimitriadis, C; Marampouti, C; Calò, A; Franco, Di A; Giakoumi, S; Franco, Di E; Lorenzo, Di M; Gerovasileiou, V; Guidetti, P; Pey, A; Sini, M; Sourbès, L

Evaluating the long term effectiveness of a Mediterranean marine protected area to tackle the effects of invasive and range expanding herbivorous fish on rocky reefs Journal Article

Marine Environmental Research, 193 , pp. 106293, 2025, ISSN: 01411136.

Links | BibTeX

Kourkouta, Chara; Tsipourlianos, Andreas; Papandroulakis, Nikos; Moutou, Katerina A; Koumoundouros, George

Ray‐Resorption Syndrome in European Seabass, textitDicentrarchus labrax (Linnaeus, 1758) Journal Article

Journal of Fish Diseases, 48 (2), pp. e14042, 2025, ISSN: 0140-7775, 1365-2761.

Abstract | Links | BibTeX

@article{kourkouta_rayresorption_2025,
title = {Ray‐Resorption Syndrome in European Seabass, textitDicentrarchus labrax (Linnaeus, 1758)},
author = {Chara Kourkouta and Andreas Tsipourlianos and Nikos Papandroulakis and Katerina A Moutou and George Koumoundouros},
url = {https://onlinelibrary.wiley.com/doi/10.1111/jfd.14042
https://imbbc.hcmr.gr/wp-content/uploads/2025/01/2025-Kourkouta-JFD-4.pdf},
doi = {10.1111/jfd.14042},
issn = {0140-7775, 1365-2761},
year = {2025},
date = {2025-01-13},
urldate = {2025-01-13},
journal = {Journal of Fish Diseases},
volume = {48},
number = {2},
pages = {e14042},
abstract = {ABSTRACT

Fin abnormalities are common in reared fish. They mainly consist of partial to complete lack of rays and severe abnormalities of fin‐supporting skeletal elements, which develop during the larval stage, up to the completion of fin skeleton ontogeny. This study reports a new abnormal condition, ray‐resorption syndrome (RSS), which developed after the completion of fin ontogeny in initially normal European seabass larvae, leading to extensive loss of fin rays. At 49 days post‐fertilisation (dpf) (15 mm standard length, SL) all fish presented normal fins. However, nineteen days later (21 mm SL), significant fin damage, characterised by ray loss or fractures, was observed in all studied populations. The dorsal fin was most affected (55%–84%), followed by the pelvic (27%–53%) and anal fins (7%–17%). Microscopically, multiple non‐mineralised areas resembling resorption lacunae were evident along all fin lepidotrichia. By 98 dpf (40 mm SL), the fin‐ray loss had advanced, reaching its higher frequency in the dorsal (74%–83%) and caudal fins (71%–94%). Gene expression analysis revealed a significant upregulation of
bglap
(osteoblast maturation marker),
acp5a
(osteoclast maturation marker) and
mmp13a
(extracellular‐matrix remodelling marker) in RSS specimens. The results are discussed in respect of the possible causative factors of RSS.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Bušelić, Ivana; Trumbić, Željka; Hrabar, Jerko; Lepen-Pleić, Ivana; Šegvić-Bubić, Tanja; Kaitetzidou, Elisavet; Tibaldi, Emilio; Bočina, Ivana; Grubišić, Leon; Sarropoulou, Elena

Unravelling the intricate language of fish guts: Impact of plant-based vs. plant-insect-poultry-based diets on intestinal pathways in European seabass Journal Article

594 , pp. 741385, 2025, ISSN: 00448486.

Links | BibTeX

Pavloudi, Christina; Santi, Ioulia; Azua, Iñigo; Baña, Zuriñe; Bastianini, Mauro; Belser, Caroline; Bilbao, Jone; Bitz-Thorsen, Julie; Broudin, Caroline; Camusat, Mathieu; Cancio, Ibon; Caray-Counil, Louis; Casotti, Raffaella; Castel, Jade; Comtet, Thierry; Cox, Cymon; Daguin, Claire; Cerio, Oihane Díaz De; Exter, Katrina; Fauvelot, Cécile; Frada, Miguel; Galand, Pierre; Garczarek, Laurence; Fernández, Jose González; Guillou, Laure; Hablützel, Pascal; Heynderickx, Hanneloor; Houbin, Céline; Kervella, Anne; Krystallas, Apostolos; Lagaisse, Rune; Laroquette, Arnaud; Lescure, Lyvia; Lopes, Eva; Loulakaki, Melina; Louro, Bruno; Magalhaes, Catarina; Maidanou, Maria; Margiotta, Francesca; Montresor, Marina; Not, Fabrice; Paredes, Estefanía; Percopo, Isabella; Péru, Erwan; Poulain, Julie; Præbel, Kim; Rigaut-Jalabert, Fabienne; Romac, Sarah; Stavroulaki, Melanthia; Troncoso, Jesús Souza; Thiébaut, Eric; Thomas, Wilfried; Tkacz, Andrzej; Trano, Anna Chiara; Wincker, Patrick; Pade, Nicolas

First release of the European marine omics biodiversity observation network (EMO BON) shotgun metagenomics data from water and sediment samples Journal Article

Biodiversity Data Journal, 13 , pp. e143585, 2025, ISSN: 1314-2828, 1314-2836.

Abstract | Links | BibTeX

@article{pavloudi_first_2025,
title = {First release of the European marine omics biodiversity observation network (EMO BON) shotgun metagenomics data from water and sediment samples},
author = {Christina Pavloudi and Ioulia Santi and Iñigo Azua and Zuriñe Baña and Mauro Bastianini and Caroline Belser and Jone Bilbao and Julie Bitz-Thorsen and Caroline Broudin and Mathieu Camusat and Ibon Cancio and Louis Caray-Counil and Raffaella Casotti and Jade Castel and Thierry Comtet and Cymon Cox and Claire Daguin and Oihane Díaz De Cerio and Katrina Exter and Cécile Fauvelot and Miguel Frada and Pierre Galand and Laurence Garczarek and Jose González Fernández and Laure Guillou and Pascal Hablützel and Hanneloor Heynderickx and Céline Houbin and Anne Kervella and Apostolos Krystallas and Rune Lagaisse and Arnaud Laroquette and Lyvia Lescure and Eva Lopes and Melina Loulakaki and Bruno Louro and Catarina Magalhaes and Maria Maidanou and Francesca Margiotta and Marina Montresor and Fabrice Not and Estefanía Paredes and Isabella Percopo and Erwan Péru and Julie Poulain and Kim Præbel and Fabienne Rigaut-Jalabert and Sarah Romac and Melanthia Stavroulaki and Jesús Souza Troncoso and Eric Thiébaut and Wilfried Thomas and Andrzej Tkacz and Anna Chiara Trano and Patrick Wincker and Nicolas Pade},
url = {https://bdj.pensoft.net/article/143585/
/wp-content/uploads/2025/04/2025-Pavloudi-BDJ-17.pdf},
doi = {10.3897/BDJ.13.e143585},
issn = {1314-2828, 1314-2836},
year = {2025},
date = {2025-01-01},
urldate = {2025-04-03},
journal = {Biodiversity Data Journal},
volume = {13},
pages = {e143585},
abstract = {The European Marine Omics Biodiversity Observation Network (EMO BON) is an initiative of the European Marine Biological Resource Centre (EMBRC) to establish a persistent genomic observatory amongst designated European coastal marine sites, sharing the same protocols for sampling and data curation. Environmental samples are collected from the water column and, at some sites, soft sediments and hard substrates (Autonomous Reef Monitoring Structures - ARMS), together with a set of mandatory and discretionary metadata (including Essential Ocean Variables - EOVs). Samples are collected following standardised protocols at regular and specified intervals and sequenced in large six-monthly batches at a centralised sequencing facility. The use of standard operating procedures (SOPs) during data collection, library preparation and sequencing aims to provide uniformity amongst the data collected from the sites. Coupled with strict adherence to open and FAIR (Findable, Accessible, Interoperable, Reusable) data principles, this ensures maximum comparability amongst samples and enhances reusability and interoperability of the data with other data sources. The observatory network was launched in June 2021, when the first sampling campaign took place.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Idan, Tal; Shefer, Sigal; Chatzigeorgiou, Giorgos; Gerovasileiou, Vasilis; Goren, Liron

Testing the effect of host availability on endobiont diversity: proposing the single hotel hypothesis Journal Article

Scientific Reports, 15 (1), pp. 21717, 2025, ISSN: 2045-2322.

Links | BibTeX

2024

Voskakis, Dimitris; Kelasidi, Eleni; Papandroulakis, Nikos

Design and Development of Underwater Robotic Arm for Automated Camera Calibration for Aquatic Environment Inproceedings

2024 10th International Conference on Automation, Robotics and Applications (ICARA), pp. 50–56, IEEE, Athens, Greece, 2024, ISBN: 9798350394245.

Links | BibTeX

Mladineo, Ivona; Hrabar, Jerko; Trumbić, Željka; Rasouli-Dogaheh, Somayeh; Beraldo, Paola; Rigos, George; Palenzuela, Oswado; Sitjà-Bobadilla, Ariadna

Mediterranean-wide transfer of the polyopisthocotylean Sparicotyle chrysophrii between wild sparids and farmed gilthead seabream (Sparus aurata) inferred by ddRAD loci Journal Article

Aquaculture, 598 , pp. 741997, 2024, ISSN: 00448486.

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Livanou, Eleni; Sauzède, Raphaëlle; Psarra, Stella; Mandalakis, Manolis; Dall’Olmo, Giorgio; Brewin, Robert J W; Raitsos, Dionysios E

Evaluating MULTIOBS Chlorophyll-a with Ground-Truth Observations in the Eastern Mediterranean Sea Journal Article

Remote Sensing, 16 (24), pp. 4705, 2024, ISSN: 2072-4292.

Abstract | Links | BibTeX

Apostolaki, Eugenia T; Lavery, Paul S; Litsi‐Mizan, Victoria; Serrano, Eduard; Inostroza, Karina; Gerakaris, Vasilis; Dailianis, Thanos; Glampedakis, Julius; Holitzki, Tara; Johnson, Erik; Mateo, Miguel A; Serrano, Oscar

Patterns of Carbon and Nitrogen Accumulation in Seagrass ( textitPosidonia oceanica ) Meadows of the Eastern Mediterranean Sea Journal Article

Journal of Geophysical Research: Biogeosciences, 129 (12), pp. e2024JG008163, 2024, ISSN: 2169-8953, 2169-8961.

Abstract | Links | BibTeX

@article{apostolaki_patterns_2024,
title = {Patterns of Carbon and Nitrogen Accumulation in Seagrass ( textitPosidonia oceanica ) Meadows of the Eastern Mediterranean Sea},
author = {Eugenia T Apostolaki and Paul S Lavery and Victoria Litsi‐Mizan and Eduard Serrano and Karina Inostroza and Vasilis Gerakaris and Thanos Dailianis and Julius Glampedakis and Tara Holitzki and Erik Johnson and Miguel A Mateo and Oscar Serrano},
url = {https://agupubs.onlinelibrary.wiley.com/doi/10.1029/2024JG008163
https://imbbc.hcmr.gr/wp-content/uploads/2024/12/2024-Apostolaki-JGR-BiosSci-47-1.pdf},
doi = {10.1029/2024JG008163},
issn = {2169-8953, 2169-8961},
year = {2024},
date = {2024-12-18},
urldate = {2024-12-18},
journal = {Journal of Geophysical Research: Biogeosciences},
volume = {129},
number = {12},
pages = {e2024JG008163},
abstract = {Abstract

The variability in stocks and accumulation rates of organic carbon (C
org
), nitrogen (N), and carbonate (CaCO
3
) was studied in fifteen
Posidonia oceanica
meadows spread throughout the South Aegean Sea (Greece). In addition, the abiotic and biotic drivers determining the pattern of variability in the accumulation rates were assessed by exploring the influence of sediment characteristics, seagrass traits, and environmental settings. The meadows supported on average (±STDEV) 14.6 ± 5.0 kg C
org
m
−2
, 0.47 ± 0.17 kg N m
−2
, and 249 ± 210 kg CaCO
3
m
−2
in the top meter of their sediments, with mean accumulation rates over the last 500 years of 33.6 ± 23.6 g C
org
m
−2
yr
−1
, 1.00 ± 0.62 g N m
−2
yr
−1
, and 405 ± 336 g CaCO
3
m
−2
yr
−1
across sites. A redundancy analysis (RDA) explained 70% of the variation in C
org
, N, and CaCO
3
accumulation rates, with three sediment characteristics (i.e., sediment C
org
:N and C
org
:C
inorg
ratios and
P
.
oceanica
contribution to the sediment C
org
pool) emerging as the primary set of factors shaping the accumulation of matter, followed by seagrass traits (i.e., leaf biomass and rhizome elongation) and environmental variables (i.e., suspended organic matter). The high degree of variability within the region emphasizes the need for fine‐scale assessments to understand the local conditions influencing sequestration. Our findings underscored the critical role of seagrass meadows in carbon and nitrogen sequestration in the region, urging conservation efforts to protect these ecosystems and prevent potential losses of stored carbon and nitrogen following seagrass degradation.

,
Plain Language Summary

In the present study, we explored the abiotic and biotic factors influencing the accumulation patterns of carbon, nitrogen, and carbonate in 15 seagrass (
Posidonia oceanica
) meadows across the South Aegean Sea (Greece). Sediment characteristics (i.e., sediment C
org
:N and C
org
:C
inorg
ratios and
P
.
ocenica
contribution to the sediment C
org
pool) mainly drove the pattern of carbon, nitrogen, and carbonate accumulation, with seagrass traits (i.e., leaf biomass and rhizome elongation) and environmental conditions (i.e., suspended organic matter) contributing to a lesser extent. Overall, seagrass meadows play a crucial role in storing carbon and nitrogen in the studied region, serving as important natural sinks, and thus, protecting these ecosystems is important to prevent the loss of these stored elements. Additionally, understanding the factors that shape the variability in
Posidonia oceanica
storage capacity is crucial for management and conservation efforts.

,
Key Points

Seagrass (
Posidonia oceanica
) meadows of the South Aegean Sea (Greece) represent important natural sinks for organic carbon, nitrogen, and carbonate.

The accumulation pattern of organic carbon, nitrogen, and carbonate in the underlying seagrass sediments was mainly driven by sediment characteristics, followed by seagrass traits and environmental conditions.

Fine‐scale assessments are crucial for understanding the local factors shaping the storage capacity of seagrass meadows to support management decisions.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Abstract

The variability in stocks and accumulation rates of organic carbon (C
org
), nitrogen (N), and carbonate (CaCO
3
) was studied in fifteen
Posidonia oceanica
meadows spread throughout the South Aegean Sea (Greece). In addition, the abiotic and biotic drivers determining the pattern of variability in the accumulation rates were assessed by exploring the influence of sediment characteristics, seagrass traits, and environmental settings. The meadows supported on average (±STDEV) 14.6 ± 5.0 kg C
org
m
−2
, 0.47 ± 0.17 kg N m
−2
, and 249 ± 210 kg CaCO
3
m
−2
in the top meter of their sediments, with mean accumulation rates over the last 500 years of 33.6 ± 23.6 g C
org
m
−2
yr
−1
, 1.00 ± 0.62 g N m
−2
yr
−1
, and 405 ± 336 g CaCO
3
m
−2
yr
−1
across sites. A redundancy analysis (RDA) explained 70% of the variation in C
org
, N, and CaCO
3
accumulation rates, with three sediment characteristics (i.e., sediment C
org
:N and C
org
:C
inorg
ratios and
P
.
oceanica
contribution to the sediment C
org
pool) emerging as the primary set of factors shaping the accumulation of matter, followed by seagrass traits (i.e., leaf biomass and rhizome elongation) and environmental variables (i.e., suspended organic matter). The high degree of variability within the region emphasizes the need for fine‐scale assessments to understand the local conditions influencing sequestration. Our findings underscored the critical role of seagrass meadows in carbon and nitrogen sequestration in the region, urging conservation efforts to protect these ecosystems and prevent potential losses of stored carbon and nitrogen following seagrass degradation.

,
Plain Language Summary

In the present study, we explored the abiotic and biotic factors influencing the accumulation patterns of carbon, nitrogen, and carbonate in 15 seagrass (
Posidonia oceanica
) meadows across the South Aegean Sea (Greece). Sediment characteristics (i.e., sediment C
org
:N and C
org
:C
inorg
ratios and
P
.
ocenica
contribution to the sediment C
org
pool) mainly drove the pattern of carbon, nitrogen, and carbonate accumulation, with seagrass traits (i.e., leaf biomass and rhizome elongation) and environmental conditions (i.e., suspended organic matter) contributing to a lesser extent. Overall, seagrass meadows play a crucial role in storing carbon and nitrogen in the studied region, serving as important natural sinks, and thus, protecting these ecosystems is important to prevent the loss of these stored elements. Additionally, understanding the factors that shape the variability in
Posidonia oceanica
storage capacity is crucial for management and conservation efforts.

,
Key Points

Seagrass (
Posidonia oceanica
) meadows of the South Aegean Sea (Greece) represent important natural sinks for organic carbon, nitrogen, and carbonate.

The accumulation pattern of organic carbon, nitrogen, and carbonate in the underlying seagrass sediments was mainly driven by sediment characteristics, followed by seagrass traits and environmental conditions.

Fine‐scale assessments are crucial for understanding the local factors shaping the storage capacity of seagrass meadows to support management decisions.

Rotter, Ana; Varamogianni-Mamatsi, Despoina; Pobirk, Alenka Zvonar; Matjaž, Mirjam Gosenca; Cueto, Mercedes; Díaz-Marrero, Ana R; Jónsdóttir, Rósa; Sveinsdóttir, Kolbrún; Catalá, Teresa S; Romano, Giovanna; Guler, Bahar Aslanbay; Atak, Eylem; Zrimec, Maja Berden; Bosch, Daniel; Deniz, Irem; Gaudêncio, Susana P; Grigalionyte-Bembič, Ernesta; Klun, Katja; Zidar, Luen; Rius, Anna Coll; Baebler, Špela; Bilela, Lada Lukić; Rinkevich, Baruch; Mandalakis, Manolis

Marine cosmetics and the blue bioeconomy: From sourcing to success stories Journal Article

iScience, 27 (12), pp. 111339, 2024, ISSN: 25890042.

Links | BibTeX

Skliros, Dimitrios; Kostakou, Maria; Kokkari, Constantina; Tsertou, Maria Ioanna; Pavloudi, Christina; Zafeiropoulos, Haris; Katharios, Pantelis; Flemetakis, Emmanouil

Unveiling Emerging Opportunistic Fish Pathogens in Aquaculture: A Comprehensive Seasonal Study of Microbial Composition in Mediterranean Fish Hatcheries Journal Article

Microorganisms, 12 (11), pp. 2281, 2024, ISSN: 2076-2607.

Abstract | Links | BibTeX

@article{skliros_unveiling_2024,
title = {Unveiling Emerging Opportunistic Fish Pathogens in Aquaculture: A Comprehensive Seasonal Study of Microbial Composition in Mediterranean Fish Hatcheries},
author = {Dimitrios Skliros and Maria Kostakou and Constantina Kokkari and Maria Ioanna Tsertou and Christina Pavloudi and Haris Zafeiropoulos and Pantelis Katharios and Emmanouil Flemetakis},
url = {https://www.mdpi.com/2076-2607/12/11/2281
https://imbbc.hcmr.gr/wp-content/uploads/2024/11/2024-Skliros-microorganisms-45.pdf},
doi = {10.3390/microorganisms12112281},
issn = {2076-2607},
year = {2024},
date = {2024-11-12},
urldate = {2024-11-12},
journal = {Microorganisms},
volume = {12},
number = {11},
pages = {2281},
abstract = {The importance of microbial communities in fish hatcheries for fish health and welfare has been recognized, with several studies mapping these communities during healthy rearing conditions and disease outbreaks. In this study, we analyzed the bacteriome of the live feeds, such as microalgae, rotifers, and Artemia, used in fish hatcheries that produce Mediterranean species. Our goal was to provide baseline information about their structure, emphasizing in environmental putative fish pathogenic bacteria. We conducted 16S rRNA amplicon Novaseq sequencing for our analysis, and we inferred 46,745 taxonomically annotated ASVs. Results showed that incoming environmental water plays a significant role in the presence of important taxa that constitute presumptive pathogens. Bio-statistical analyses revealed a relatively stable bacteriome among seasonal samplings for every hatchery but a diverse bacteriome between sampling stations and a distinct core bacteriome for each hatchery. Analysis of putative opportunistic fish pathogenic genera revealed some co-occurrence correlation events and a high average relative abundance of Vibrio, Tenacibaculum, and Photobacterium genera in live feeds, reaching a grand mean average of up to 7.3% for the hatchery of the Hellenic Center of Marine Research (HCMR), 12% for Hatchery A, and 11.5% for Hatchery B. Mapping the bacteriome in live feeds is pivotal for understanding the marine environment and distinct aquaculture practices and can guide improvements in hatchery management, enhancing fish health and sustainability in the Mediterranean region.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

Lanza-Arroyo, Pablo; Sempere-Valverde, Juan; Digenis, Markos; Remón, José Miguel; Moreno, Diego; Barrajón, Agustín; Linde, Antonio De La; Arroyo, Maria Del Carmen; Fernández-Casado, Manuel; Mallofret, Eugenio; Sánchez-Tocino, Luis; Gerovasileiou, Vasilis; Navarro-Barranco, Carlos

Baseline for marine cave monitoring strategies in the Alboran Sea using modified Cave Ecosystem-Based Quality Index (CavEBQI) Journal Article

Marine Pollution Bulletin, 209 , pp. 117065, 2024, ISSN: 0025326X.

Links | BibTeX