Results for Author Kotzamanis (Yannis) Ioannis :
2025 |
Dritsas, Panagiotis; Patsialou, Stefania; Kampantais, Dimitrios; Roussos, Efstratios; Kotzamanis, Yannis; Tekerlekopoulou, Athanasia; Vayenas, Dimitris V; Aggelis, George Investigating the Potential of Newly Isolated Microalgae Strains from the Ionian Sea (Greece) Cultured in an Open Raceway Pond Journal Article Applied Sciences, 15 (12), pp. 6680, 2025, ISSN: 2076-3417. @article{dritsas_investigating_2025, title = {Investigating the Potential of Newly Isolated Microalgae Strains from the Ionian Sea (Greece) Cultured in an Open Raceway Pond}, author = {Panagiotis Dritsas and Stefania Patsialou and Dimitrios Kampantais and Efstratios Roussos and Yannis Kotzamanis and Athanasia Tekerlekopoulou and Dimitris V Vayenas and George Aggelis}, url = {https://www.mdpi.com/2076-3417/15/12/6680 /wp-content/uploads/2025/07/2025-Dritsas-AppSci-35.pdf}, doi = {10.3390/app15126680}, issn = {2076-3417}, year = {2025}, date = {2025-07-07}, urldate = {2025-07-10}, journal = {Applied Sciences}, volume = {15}, number = {12}, pages = {6680}, abstract = {Microalgae are a subject of interest not only for fundamental research but for various biotechnological applications as well. In this study, the ability of newly isolated strains, i.e., Picochlorum costavermella VAS2.5, Picochlorum oklahomense SAG4.4, Microchloropsis gaditana VON5.3, and Nephroselmis pyriformis PAT2.7, to grow when cultured in an open raceway pond under laboratory conditions and produce various metabolites of high-added value was evaluated. N. pyriformis PAT2.7 and P. costavermella VAS2.5 were the greatest in biomass production (exceeding 0.4 g/L), while P. costavermella VAS2.5 and M. gaditana VON5.3 were the greatest in lipid production (reaching approximately 18%, wt/wt). The lipid fraction of glycolipids and sphingolipids was predominant (43.6–55.4%, wt/wt), followed by neutral lipids (27.1–50.1%, wt/wt) and phospholipids (6.9–17.4%, wt/wt). Picochlora and M. gaditana VON5.3 lipids were rich in Δ5,8,11,14,17C20:5 and/or Δ9,12,15C18:3, while N. pyriformis PAT2.7 synthesized Δ9C16:1 in large quantities (30–40%, wt/wt). All strains showed remarkable yields in polysaccharide and protein production, demonstrated a well-balanced amino acid profile, and synthesized pigments in amounts comparable to other studies. The biochemical profiles of these strains showcased their suitability for use primarily in the aquaculture sector.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Microalgae are a subject of interest not only for fundamental research but for various biotechnological applications as well. In this study, the ability of newly isolated strains, i.e., Picochlorum costavermella VAS2.5, Picochlorum oklahomense SAG4.4, Microchloropsis gaditana VON5.3, and Nephroselmis pyriformis PAT2.7, to grow when cultured in an open raceway pond under laboratory conditions and produce various metabolites of high-added value was evaluated. N. pyriformis PAT2.7 and P. costavermella VAS2.5 were the greatest in biomass production (exceeding 0.4 g/L), while P. costavermella VAS2.5 and M. gaditana VON5.3 were the greatest in lipid production (reaching approximately 18%, wt/wt). The lipid fraction of glycolipids and sphingolipids was predominant (43.6–55.4%, wt/wt), followed by neutral lipids (27.1–50.1%, wt/wt) and phospholipids (6.9–17.4%, wt/wt). Picochlora and M. gaditana VON5.3 lipids were rich in Δ5,8,11,14,17C20:5 and/or Δ9,12,15C18:3, while N. pyriformis PAT2.7 synthesized Δ9C16:1 in large quantities (30–40%, wt/wt). All strains showed remarkable yields in polysaccharide and protein production, demonstrated a well-balanced amino acid profile, and synthesized pigments in amounts comparable to other studies. The biochemical profiles of these strains showcased their suitability for use primarily in the aquaculture sector. |
Wei, Xiao-Lei; Hao, Zhi-Wei; Kotzamanis, Yannis P; Zhang, Tian-Hua; Liu, Zhi-Bo; Yang, Hong; Luo, Zhi Aquaculture Reports, 42 , pp. 102739, 2025, ISSN: 23525134. @article{wei_dietary_2025, title = {Dietary iron oxide (Fe2O3) nanoparticles modulate growth performance, body composition, mineral content and intestinal health of yellow catfish juveniles (Pelteobagrus fulvidraco)}, author = {Xiao-Lei Wei and Zhi-Wei Hao and Yannis P Kotzamanis and Tian-Hua Zhang and Zhi-Bo Liu and Hong Yang and Zhi Luo}, url = {https://linkinghub.elsevier.com/retrieve/pii/S2352513425001255 /wp-content/uploads/2025/03/2025-Wei-AquaReports-16.pdf}, doi = {10.1016/j.aqrep.2025.102739}, issn = {23525134}, year = {2025}, date = {2025-03-31}, urldate = {2025-03-31}, journal = {Aquaculture Reports}, volume = {42}, pages = {102739}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2024 |
Roussos, Efstratios; Triantaphyllidis, George; Ilia, Vassiliki; Tsagarakis, Konstantinos; Machias, Athanasios; Tziveleka, Leto-Aikaterini; Roussis, Vassilios; Ioannou, Efstathia; Kotzamanis, Yannis Status of Fishery Discards and By-Products in Greece and Potential Valorization Scenarios towards a National Exploitation Master Plan Journal Article Marine Drugs, 22 (6), pp. 264, 2024, ISSN: 1660-3397. @article{roussos_status_2024, title = {Status of Fishery Discards and By-Products in Greece and Potential Valorization Scenarios towards a National Exploitation Master Plan}, author = {Efstratios Roussos and George Triantaphyllidis and Vassiliki Ilia and Konstantinos Tsagarakis and Athanasios Machias and Leto-Aikaterini Tziveleka and Vassilios Roussis and Efstathia Ioannou and Yannis Kotzamanis}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2024/06/2024-Roussos-Marinedrugs-28.pdf https://www.mdpi.com/1660-3397/22/6/264}, doi = {10.3390/md22060264}, issn = {1660-3397}, year = {2024}, date = {2024-06-17}, urldate = {2024-06-17}, journal = {Marine Drugs}, volume = {22}, number = {6}, pages = {264}, abstract = {The valorization of aquaculture/fishery processing by-products, as well as unavoidable/unwanted catches and discards in Greece, is currently an underutilized activity despite the fact that there are several best practices in Northern Europe and overseas. One of the main challenges is to determine whether the available quantities for processing are sufficient to warrant the valorization of discards and fish side streams. This is the first attempt to systematically record and analyze the available quantities of fish by-products and discards in Greece spatially and temporally in an effort to create a national exploitation Master Plan for the valorization of this unavoidable and unwanted biomass. A thorough survey conducted within the VIOAXIOPIO project unveiled a substantial biomass of around 19,000 tonnes annually that could be harnessed for valorization. Furthermore, the production of various High-Added-Value Biomolecules (HAVBs) was investigated and experimental trials were conducted to assess the potential yields, with the collected data used to formulate four valorization scenarios.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The valorization of aquaculture/fishery processing by-products, as well as unavoidable/unwanted catches and discards in Greece, is currently an underutilized activity despite the fact that there are several best practices in Northern Europe and overseas. One of the main challenges is to determine whether the available quantities for processing are sufficient to warrant the valorization of discards and fish side streams. This is the first attempt to systematically record and analyze the available quantities of fish by-products and discards in Greece spatially and temporally in an effort to create a national exploitation Master Plan for the valorization of this unavoidable and unwanted biomass. A thorough survey conducted within the VIOAXIOPIO project unveiled a substantial biomass of around 19,000 tonnes annually that could be harnessed for valorization. Furthermore, the production of various High-Added-Value Biomolecules (HAVBs) was investigated and experimental trials were conducted to assess the potential yields, with the collected data used to formulate four valorization scenarios. |
Tzortzatos, Odysseas-Panagiotis; Toubanaki, Dimitra K; Kolygas, Markos N; Kotzamanis, Yannis; Roussos, Efstratios; Bakopoulos, Vasileios; Chatzopoulos, Achilleas; Athanassopoulou, Fotini; Karagouni, Evdokia Dietary Artemisia arborescens Supplementation Effects on Growth, Oxidative Status, and Immunity of Gilthead Seabream (Sparus aurata L.) Journal Article Animals, 14 (8), pp. 1161, 2024, ISSN: 2076-2615. @article{tzortzatos_dietary_2024, title = {Dietary Artemisia arborescens Supplementation Effects on Growth, Oxidative Status, and Immunity of Gilthead Seabream (Sparus aurata L.)}, author = {Odysseas-Panagiotis Tzortzatos and Dimitra K Toubanaki and Markos N Kolygas and Yannis Kotzamanis and Efstratios Roussos and Vasileios Bakopoulos and Achilleas Chatzopoulos and Fotini Athanassopoulou and Evdokia Karagouni}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2024/05/2024-Tzortzatos-ANIMALS-24.pdf https://www.mdpi.com/2076-2615/14/8/1161}, doi = {10.3390/ani14081161}, issn = {2076-2615}, year = {2024}, date = {2024-04-29}, urldate = {2024-05-13}, journal = {Animals}, volume = {14}, number = {8}, pages = {1161}, abstract = {Fish infectious diseases are one of the main constraints of the aquaculture sector. The use of medicinal plants provides a sustainable way of protection using safe, eco-friendly compounds in a more cost-effective way of treatment, compared to antibiotics. The aim of the present study is the assessment of Artemisia arborescens (AA) feed-supplementation effects on gilthead seabream (Sparus aurata). Fish with an average initial body weight of 109.43 ± 3.81 g, were divided into two groups based on AA feed composition (A25 and A50). Following two months of ad libitum feeding, the effect of diets on fish weight and length were measured. Fish serum and mucus were analyzed for non-specific immune parameters (nitric oxide, lysozyme, myeloperoxidase, protease-/anti-protease activity, and complement), antibody responses, oxidative stress (cytochrome P450 1A1, metallothionein), and metabolism markers (total protein, alkaline phosphatase, and glucose). Expression levels of antioxidants (sod1, gpx1), cytokines (il-1b, il-10, tfgb1, and tnfa), hepcidin, and heat shock protein grp75 genes were measured in spleen samples. A results analysis indicated that A. arborescens use as a feed supplement has a compromised positive effect on the growth performance, immune response, and blood parameters of gilthead seabream. Overall, the suitability of A. arborescens as an efficient food supplement for gilthead seabream health improvement was investigated, setting the basis for its application assessment in Mediterranean aquaculture.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Fish infectious diseases are one of the main constraints of the aquaculture sector. The use of medicinal plants provides a sustainable way of protection using safe, eco-friendly compounds in a more cost-effective way of treatment, compared to antibiotics. The aim of the present study is the assessment of Artemisia arborescens (AA) feed-supplementation effects on gilthead seabream (Sparus aurata). Fish with an average initial body weight of 109.43 ± 3.81 g, were divided into two groups based on AA feed composition (A25 and A50). Following two months of ad libitum feeding, the effect of diets on fish weight and length were measured. Fish serum and mucus were analyzed for non-specific immune parameters (nitric oxide, lysozyme, myeloperoxidase, protease-/anti-protease activity, and complement), antibody responses, oxidative stress (cytochrome P450 1A1, metallothionein), and metabolism markers (total protein, alkaline phosphatase, and glucose). Expression levels of antioxidants (sod1, gpx1), cytokines (il-1b, il-10, tfgb1, and tnfa), hepcidin, and heat shock protein grp75 genes were measured in spleen samples. A results analysis indicated that A. arborescens use as a feed supplement has a compromised positive effect on the growth performance, immune response, and blood parameters of gilthead seabream. Overall, the suitability of A. arborescens as an efficient food supplement for gilthead seabream health improvement was investigated, setting the basis for its application assessment in Mediterranean aquaculture. |
Zhao, Tao; Xu, Jie-Jie; Kotzamanis, Yannis P; Zhang, Dian-Guang; Xu, Yi-Chuang; Zheng, Hua; Han, Ya-Kang; Luo, Zhi Aquaculture, 582 , pp. 740569, 2024, ISSN: 00448486. @article{zhao_effects_2024, title = {Effects of dietary citric acid on growth performance, mineral status, body and muscle composition, muscle growth and mTOR signaling in yellow catfish Pelteobagrus fulvidraco fed with low-manganese diets}, author = {Tao Zhao and Jie-Jie Xu and Yannis P Kotzamanis and Dian-Guang Zhang and Yi-Chuang Xu and Hua Zheng and Ya-Kang Han and Zhi Luo}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2024/03/2024-Kotzamanis-et-al-AQUA-16.pdf https://linkinghub.elsevier.com/retrieve/pii/S0044848624000309}, doi = {10.1016/j.aquaculture.2024.740569}, issn = {00448486}, year = {2024}, date = {2024-03-22}, urldate = {2024-03-22}, journal = {Aquaculture}, volume = {582}, pages = {740569}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Zhao, Tao; Zheng, Hua; Xu, Jie-Jie; Pantopoulos, Kostas; Xu, Yi-Chuang; Liu, Lu-Lu; Lei, Xi-Jun; Kotzamanis, Yannis P; Luo, Zhi MnO2 nanoparticles trigger hepatic lipotoxicity and mitophagy via mtROS-dependent Hsf1Ser326 phosphorylation Journal Article Free Radical Biology and Medicine, 210 , pp. 390–405, 2024, ISSN: 08915849. @article{zhao_mno2_2024, title = {MnO2 nanoparticles trigger hepatic lipotoxicity and mitophagy via mtROS-dependent Hsf1Ser326 phosphorylation}, author = {Tao Zhao and Hua Zheng and Jie-Jie Xu and Kostas Pantopoulos and Yi-Chuang Xu and Lu-Lu Liu and Xi-Jun Lei and Yannis P Kotzamanis and Zhi Luo}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2024/03/2024-Kotzamanis-et-al-pre-print-FRBM-15.pdf https://linkinghub.elsevier.com/retrieve/pii/S0891584923011383}, doi = {10.1016/j.freeradbiomed.2023.11.037}, issn = {08915849}, year = {2024}, date = {2024-03-19}, urldate = {2024-03-19}, journal = {Free Radical Biology and Medicine}, volume = {210}, pages = {390--405}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2023 |
Rotter, Ana; Giannakourou, Antonia; García, Jesús Argente E; Quero, Grazia Marina; Auregan, Charlène; Triantaphyllidis, George; Venetsanopoulou, Amalia; Carolis, Roberta De; Efstratiou, Chrysa; Aboal, Marina; Abad, María Ángeles Esteban; Grigalionyte-Bembič, Ernesta; Kotzamanis, Yannis; Kovač, Mate; Čmelar, Maja Ljubić; Luna, Gian Marco; Aguilera, Cristóbal; Fernández, Francisco Gabriel Acién; Pinchetti, Juan Luis Gómez; Manzo, Sonia; Milašinčić, Iva; Nadarmija, Antun; Parrella, Luisa; Pinat, Massimiliano; Roussos, Efstratios; Ruel, Colin; Salvatori, Elisabetta; Vázquez, Francisco Javier Sánchez; García, María Semitiel; Gómez, Antonio Skarmeta F; Ulčar, Jan; Chiavetta, Cristian Identification of Marine Biotechnology Value Chains with High Potential in the Northern Mediterranean Region Journal Article Marine Drugs, 21 (7), pp. 416, 2023, ISSN: 1660-3397. @article{rotter_identification_2023, title = {Identification of Marine Biotechnology Value Chains with High Potential in the Northern Mediterranean Region}, author = {Ana Rotter and Antonia Giannakourou and Jesús E Argente García and Grazia Marina Quero and Charlène Auregan and George Triantaphyllidis and Amalia Venetsanopoulou and Roberta De Carolis and Chrysa Efstratiou and Marina Aboal and María Ángeles Esteban Abad and Ernesta Grigalionyte-Bembič and Yannis Kotzamanis and Mate Kovač and Maja Ljubić Čmelar and Gian Marco Luna and Cristóbal Aguilera and Francisco Gabriel Acién Fernández and Juan Luis Gómez Pinchetti and Sonia Manzo and Iva Milašinčić and Antun Nadarmija and Luisa Parrella and Massimiliano Pinat and Efstratios Roussos and Colin Ruel and Elisabetta Salvatori and Francisco Javier Sánchez Vázquez and María Semitiel García and Antonio F Skarmeta Gómez and Jan Ulčar and Cristian Chiavetta}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/09/2023-Rotter-MarDrugs-50.pdf https://www.mdpi.com/1660-3397/21/7/416}, doi = {10.3390/md21070416}, issn = {1660-3397}, year = {2023}, date = {2023-09-25}, urldate = {2023-09-27}, journal = {Marine Drugs}, volume = {21}, number = {7}, pages = {416}, abstract = {Marine (blue) biotechnology is an emerging field enabling the valorization of new products and processes with massive potential for innovation and economic growth. In the Mediterranean region, this innovation potential is not exploited as well as in other European regions due to a lack of a clear identification of the different value chains and the high fragmentation of business innovation initiatives. As a result, several opportunities to create an innovative society are being missed. To address this problem, eight Northern Mediterranean countries (Croatia, France, Greece, Italy, Montenegro, Portugal, Slovenia and Spain) established five national blue biotechnology hubs to identify and address the bottlenecks that prevent the development of marine biotechnology in the region. Following a three-step approach (1. Analysis: setting the scene; 2. Transfer: identification of promising value chains; 3. Capitalization: community creation), we identified the three value chains that are most promising for the Northern Mediterranean region: algae production for added-value compounds, integrated multi-trophic aquaculture (IMTA) and valorization aquaculture/fisheries/processing by-products, unavoidable/unwanted catches and discards. The potential for the development and the technical and non-technical skills that are necessary to advance in this exciting field were identified through several stakeholder events which provided valuable insight and feedback that should be addressed for marine biotechnology in the Northern Mediterranean region to reach its full potential.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Marine (blue) biotechnology is an emerging field enabling the valorization of new products and processes with massive potential for innovation and economic growth. In the Mediterranean region, this innovation potential is not exploited as well as in other European regions due to a lack of a clear identification of the different value chains and the high fragmentation of business innovation initiatives. As a result, several opportunities to create an innovative society are being missed. To address this problem, eight Northern Mediterranean countries (Croatia, France, Greece, Italy, Montenegro, Portugal, Slovenia and Spain) established five national blue biotechnology hubs to identify and address the bottlenecks that prevent the development of marine biotechnology in the region. Following a three-step approach (1. Analysis: setting the scene; 2. Transfer: identification of promising value chains; 3. Capitalization: community creation), we identified the three value chains that are most promising for the Northern Mediterranean region: algae production for added-value compounds, integrated multi-trophic aquaculture (IMTA) and valorization aquaculture/fisheries/processing by-products, unavoidable/unwanted catches and discards. The potential for the development and the technical and non-technical skills that are necessary to advance in this exciting field were identified through several stakeholder events which provided valuable insight and feedback that should be addressed for marine biotechnology in the Northern Mediterranean region to reach its full potential. |
2022 |
Mallioris, Panagiotis; Kotzamanis, Yannis; Vardali, Sofia; Roussos, Efstratios; Ilia, Vasiliki; Paschali, Elissavet; Kampantais, Dimitrios; Bouziotis, Dimitrios; Khadem, Alireza; Vatsos, Ioannis N Animal Feed Science and Technology, 284 , pp. 115194, 2022, ISSN: 03778401. @article{mallioris_modulation_2022, title = {Modulation of intestinal health and hepatic vacuolation in gilthead sea bream (Sparus aurata) juveniles by a mixture of dietary esterified butyrins, emulsifiers from plants and yeast extracts at low and high fish meal inclusion}, author = {Panagiotis Mallioris and Yannis Kotzamanis and Sofia Vardali and Efstratios Roussos and Vasiliki Ilia and Elissavet Paschali and Dimitrios Kampantais and Dimitrios Bouziotis and Alireza Khadem and Ioannis N Vatsos}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0377840121003801}, doi = {10.1016/j.anifeedsci.2021.115194}, issn = {03778401}, year = {2022}, date = {2022-10-26}, urldate = {2022-10-26}, journal = {Animal Feed Science and Technology}, volume = {284}, pages = {115194}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Mastoraki, Maria; Katsika, Lydia; Enes, Paula; Guerreiro, Inês; Kotzamanis, Yannis P; Gasco, Laura; Chatzifotis, Stavros; Antonopoulou, Efthimia Aquaculture, 561 , pp. 738674, 2022, ISSN: 00448486. @article{mastoraki_insect_2022, title = {Insect meals in feeds for juvenile gilthead seabream (Sparus aurata): Effects on growth, blood chemistry, hepatic metabolic enzymes, body composition and nutrient utilization}, author = {Maria Mastoraki and Lydia Katsika and Paula Enes and Inês Guerreiro and Yannis P Kotzamanis and Laura Gasco and Stavros Chatzifotis and Efthimia Antonopoulou}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/10/2022-Mastoraki-AQUApre-print-70.pdf https://linkinghub.elsevier.com/retrieve/pii/S0044848622007918}, doi = {10.1016/j.aquaculture.2022.738674}, issn = {00448486}, year = {2022}, date = {2022-10-14}, urldate = {2022-10-14}, journal = {Aquaculture}, volume = {561}, pages = {738674}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Gisbert, Enric; Luz, Ronald Kennedy; Fernández, Ignacio; Pradhan, Pravata K; Salhi, Maria; Mozanzadeh, Mansour T; Kumar, Aditya; Kotzamanis, Yannis; Castro‐Ruiz, Diana; Bessonart, Martin; Darias, Maria J Development, nutrition, and rearing practices of relevant catfish species (Siluriformes) at early stages Journal Article Reviews in Aquaculture, 14 (1), pp. 73–105, 2022, ISSN: 1753-5123, 1753-5131. @article{gisbert_development_2022, title = {Development, nutrition, and rearing practices of relevant catfish species (Siluriformes) at early stages}, author = {Enric Gisbert and Ronald Kennedy Luz and Ignacio Fernández and Pravata K Pradhan and Maria Salhi and Mansour T Mozanzadeh and Aditya Kumar and Yannis Kotzamanis and Diana Castro‐Ruiz and Martin Bessonart and Maria J Darias}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/01/2022-Kotzamanis-RAqua-preprint-2.pdf https://onlinelibrary.wiley.com/doi/10.1111/raq.12586}, doi = {10.1111/raq.12586}, issn = {1753-5123, 1753-5131}, year = {2022}, date = {2022-01-01}, urldate = {2022-01-12}, journal = {Reviews in Aquaculture}, volume = {14}, number = {1}, pages = {73--105}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2021 |
Kotzamanis, Yannis; Fawole, Femi J; Brezas, Andreas; Kumar, Vikas; Fontanillas, Ramon; Antonopoulou, Efthimia; Kouroupakis, Emmanouil; Ilia, Vassiliki Dietary lysine requirement of greater amberjack juvenile ( textitSeriola dumerili , Risso, 1810) Journal Article Aquaculture Nutrition, 27 (6), pp. 2107–2118, 2021, ISSN: 1353-5773, 1365-2095. @article{kotzamanis_dietary_2021, title = {Dietary lysine requirement of greater amberjack juvenile ( textitSeriola dumerili , Risso, 1810)}, author = {Yannis Kotzamanis and Femi J Fawole and Andreas Brezas and Vikas Kumar and Ramon Fontanillas and Efthimia Antonopoulou and Emmanouil Kouroupakis and Vassiliki Ilia}, url = {https://onlinelibrary.wiley.com/doi/10.1111/anu.13344}, doi = {10.1111/anu.13344}, issn = {1353-5773, 1365-2095}, year = {2021}, date = {2021-12-01}, urldate = {2022-01-12}, journal = {Aquaculture Nutrition}, volume = {27}, number = {6}, pages = {2107--2118}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Katsika, Lydia; Flores, Mario Huesca; Kotzamanis, Yannis; Estevez, Alicia; Chatzifotis, Stavros Animals, 11 (2), pp. 392, 2021. @article{katsika_understanding_2021, title = {Understanding the Interaction Effects between Dietary Lipid Content and Rearing Temperature on Growth Performance, Feed Utilization, and Fat Deposition of Sea Bass (Dicentrarchus labrax)}, author = {Lydia Katsika and Mario Huesca Flores and Yannis Kotzamanis and Alicia Estevez and Stavros Chatzifotis}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/02/2021-Katsika-animals-10.pdf https://www.mdpi.com/2076-2615/11/2/392}, doi = {10.3390/ani11020392}, year = {2021}, date = {2021-02-01}, urldate = {2021-02-05}, journal = {Animals}, volume = {11}, number = {2}, pages = {392}, abstract = {This study was conducted to elucidate the interaction effects of temperature and dietary lipid levels (2 × 2 factorial experiment) on the growth performance, muscle, and liver composition in adult farmed European sea bass (Dicentrarchus labrax). Two groups of fish (190 g; 60 fish per group) were distributed in 12 tanks in triplicates and kept at two different temperature regimes; one starting at 23 °C and then changed to 17 °C for 61 days, and the other starting at 17 °C and then changed to 23 °C for 39 days. Two commercial diets containing both textasciitilde44% crude protein but incorporating different dietary lipid levels, 16.5% (D16) and 20.0% (D20) (dry matter (DM)), were fed to the fish to apparent satiation; the type of diet fed to each fish group remained constant throughout the experiment. Final body weight, weight gain, and specific growth rate were significantly higher for the fish group held at 23 °C compared to the fish group at 17 °C (before the temperature changes), while the dietary fat content did not have any profound effect in both groups. Furthermore, the different temperature regimes did not affect muscle or liver composition, but, on the contrary, dietary lipids affected hepatosomatic, perivisceral fat, and visceral indexes. Feed conversion ratio and specific growth rate were not affected by the dietary lipid level. An interaction of temperature and dietary lipid content was observed in daily feed consumption (DFC) and final body weight (FBW).}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study was conducted to elucidate the interaction effects of temperature and dietary lipid levels (2 × 2 factorial experiment) on the growth performance, muscle, and liver composition in adult farmed European sea bass (Dicentrarchus labrax). Two groups of fish (190 g; 60 fish per group) were distributed in 12 tanks in triplicates and kept at two different temperature regimes; one starting at 23 °C and then changed to 17 °C for 61 days, and the other starting at 17 °C and then changed to 23 °C for 39 days. Two commercial diets containing both textasciitilde44% crude protein but incorporating different dietary lipid levels, 16.5% (D16) and 20.0% (D20) (dry matter (DM)), were fed to the fish to apparent satiation; the type of diet fed to each fish group remained constant throughout the experiment. Final body weight, weight gain, and specific growth rate were significantly higher for the fish group held at 23 °C compared to the fish group at 17 °C (before the temperature changes), while the dietary fat content did not have any profound effect in both groups. Furthermore, the different temperature regimes did not affect muscle or liver composition, but, on the contrary, dietary lipids affected hepatosomatic, perivisceral fat, and visceral indexes. Feed conversion ratio and specific growth rate were not affected by the dietary lipid level. An interaction of temperature and dietary lipid content was observed in daily feed consumption (DFC) and final body weight (FBW). |
Ragaza, Janice Alano; Hossain, Md. Sakhawat; Koshio, Shunsuke; Ishikawa, Manabu; Yokoyama, Saichiro; Kotzamanis, Yannis; Brezas, Andreas; Kumar, Vikas Aquaculture Nutrition, 27 (4), pp. 1052–1064, 2021, ISSN: 1353-5773, 1365-2095. @article{ragaza_brown_2021, title = {Brown seaweed ( textitSargassum fulvellum ) inclusion in diets with fishmeal partially replaced with soy protein concentrate for Japanese flounder ( textitParalichthys olivaceus ) juveniles}, author = {Janice Alano Ragaza and Md. Sakhawat Hossain and Shunsuke Koshio and Manabu Ishikawa and Saichiro Yokoyama and Yannis Kotzamanis and Andreas Brezas and Vikas Kumar}, url = {https://onlinelibrary.wiley.com/doi/10.1111/anu.13246}, doi = {10.1111/anu.13246}, issn = {1353-5773, 1365-2095}, year = {2021}, date = {2021-01-01}, urldate = {2021-07-26}, journal = {Aquaculture Nutrition}, volume = {27}, number = {4}, pages = {1052--1064}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2020 |
Kotzamanis, Yannis; Tsironi, Theofania; Brezas, Andreas; Grigorakis, Kriton; Ilia, Vassiliki; Vatsos, Ioannis; Romano, Nicholas; van Eys, Jan; Kumar, Vikas Scientific Reports, 10 (1), pp. 12294, 2020, ISSN: 2045-2322. @article{kotzamanis_high_2020, title = {High taurine supplementation in plant protein-based diets improves growth and organoleptic characteristics of European seabass (Dicentrarchus labrax)}, author = {Yannis Kotzamanis and Theofania Tsironi and Andreas Brezas and Kriton Grigorakis and Vassiliki Ilia and Ioannis Vatsos and Nicholas Romano and Jan van Eys and Vikas Kumar}, url = {http://www.nature.com/articles/s41598-020-69014-x}, doi = {10.1038/s41598-020-69014-x}, issn = {2045-2322}, year = {2020}, date = {2020-12-01}, urldate = {2020-08-10}, journal = {Scientific Reports}, volume = {10}, number = {1}, pages = {12294}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Mastoraki, Maria; Ferrándiz, Paula Mollá; Vardali, Sofia C; Kontodimas, Demetrius C; Kotzamanis, Yannis P; Gasco, Laura; Chatzifotis, Stavros; Antonopoulou, Efthimia Aquaculture, 528 , pp. 735511, 2020, ISSN: 00448486. @article{mastoraki_comparative_2020, title = {A comparative study on the effect of fish meal substitution with three different insect meals on growth, body composition and metabolism of European sea bass (Dicentrarchus labrax L.)}, author = {Maria Mastoraki and Paula Mollá Ferrándiz and Sofia C Vardali and Demetrius C Kontodimas and Yannis P Kotzamanis and Laura Gasco and Stavros Chatzifotis and Efthimia Antonopoulou}, url = {https://linkinghub.elsevier.com/retrieve/pii/S004484862030065X}, doi = {10.1016/j.aquaculture.2020.735511}, issn = {00448486}, year = {2020}, date = {2020-11-01}, urldate = {2020-07-06}, journal = {Aquaculture}, volume = {528}, pages = {735511}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Kotzamanis, Yannis; Kumar, Vikas; Tsironi, Theofania; Grigorakis, Kriton; Ilia, Vassiliki; Vatsos, Ioannis; Brezas, Andreas; Eys, Jan [van; Gisbert, Enric Taurine supplementation in high-soy diets affects fillet quality of European sea bass (Dicentrarchus labrax) Journal Article Aquaculture, 520 , pp. 734655, 2020, ISSN: 0044-8486. @article{kotzamanis_taurine_2020, title = {Taurine supplementation in high-soy diets affects fillet quality of European sea bass (Dicentrarchus labrax)}, author = {Yannis Kotzamanis and Vikas Kumar and Theofania Tsironi and Kriton Grigorakis and Vassiliki Ilia and Ioannis Vatsos and Andreas Brezas and Jan [van Eys and Enric Gisbert}, url = {http://www.sciencedirect.com/science/article/pii/S0044848619313900}, doi = {https://doi.org/10.1016/j.aquaculture.2019.734655}, issn = {0044-8486}, year = {2020}, date = {2020-01-01}, journal = {Aquaculture}, volume = {520}, pages = {734655}, abstract = {This study evaluated the effects of taurine supplementation to diets containing a high dietary inclusion of soybean meal and soy protein concentrate on growth performance and fillet quality of juvenile European sea bass (Dicentrarchus labrax). A control diet (C+) was produced containing high levels of fishmeal (30% FM) and soybean meal (20% SBM). Three other experimental diets were prepared to contain a lower FM inclusion (25%), and a higher amount of soy products (20% of SBM plus 12% soy protein concentrate, SPC) supplemented with three graded levels of crystalline taurine, 0.2%, 0.5% and 1.0% (T0.2, T0.5 and T1.0), respectively. A fifth diet was also prepared having a similar composition as the latter three diets but without the addition of crystalline taurine (negative control diet, C-). All diets were iso-nitrogenous (44%), iso-lipidic (20%) and iso-energetic (22 MJ kg−1) and were fed to five triplicate groups of sea bass (initial weight 86 g) over the course of a 12-week trial. Dietary taurine supplementation did not affect the growth performance and feed efficiency (P textgreater 0.05). Proximate composition of whole body and muscle were similar among groups (P textgreater 0.05). Taurine dietary supplementation had no effect on the level of intraperitoneal fat deposition (P textgreater 0.05). However, muscle taurine concentration was found to increase gradually in sea bass fed the elevated levels of taurine (P textless 0.05). Interestingly, the hardness and chewiness of the fillet, recorded by texture analysis, increased significantly at higher dietary taurine levels (P textless 0.05). The highest adhesiveness values were obtained in sea bass fed the C- diet, whereas the lowest ones were found in fish fed the T1.0 diet (P textless 0.05). No significant (P textgreater 0.05) impact of diets on texture fillet springiness and cohesiveness was found (P textgreater 0.05). In general, no significant differences were observed by the test panel, however, fish fed the diet supplemented with 1.0% taurine exhibited lower fillet elasticity, thus indicating a potential textural difference in accordance with those obtained from the texture analysis of fish muscles. The histological analysis did not indicate any differences in the gut and liver of the fish fed the experimental diets. Overall, the findings of the present study showed that 1.0% taurine supplementation in diets incorporating high levels of soy products might have a pronounced effect on flesh quality of European sea bass.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study evaluated the effects of taurine supplementation to diets containing a high dietary inclusion of soybean meal and soy protein concentrate on growth performance and fillet quality of juvenile European sea bass (Dicentrarchus labrax). A control diet (C+) was produced containing high levels of fishmeal (30% FM) and soybean meal (20% SBM). Three other experimental diets were prepared to contain a lower FM inclusion (25%), and a higher amount of soy products (20% of SBM plus 12% soy protein concentrate, SPC) supplemented with three graded levels of crystalline taurine, 0.2%, 0.5% and 1.0% (T0.2, T0.5 and T1.0), respectively. A fifth diet was also prepared having a similar composition as the latter three diets but without the addition of crystalline taurine (negative control diet, C-). All diets were iso-nitrogenous (44%), iso-lipidic (20%) and iso-energetic (22 MJ kg−1) and were fed to five triplicate groups of sea bass (initial weight 86 g) over the course of a 12-week trial. Dietary taurine supplementation did not affect the growth performance and feed efficiency (P textgreater 0.05). Proximate composition of whole body and muscle were similar among groups (P textgreater 0.05). Taurine dietary supplementation had no effect on the level of intraperitoneal fat deposition (P textgreater 0.05). However, muscle taurine concentration was found to increase gradually in sea bass fed the elevated levels of taurine (P textless 0.05). Interestingly, the hardness and chewiness of the fillet, recorded by texture analysis, increased significantly at higher dietary taurine levels (P textless 0.05). The highest adhesiveness values were obtained in sea bass fed the C- diet, whereas the lowest ones were found in fish fed the T1.0 diet (P textless 0.05). No significant (P textgreater 0.05) impact of diets on texture fillet springiness and cohesiveness was found (P textgreater 0.05). In general, no significant differences were observed by the test panel, however, fish fed the diet supplemented with 1.0% taurine exhibited lower fillet elasticity, thus indicating a potential textural difference in accordance with those obtained from the texture analysis of fish muscles. The histological analysis did not indicate any differences in the gut and liver of the fish fed the experimental diets. Overall, the findings of the present study showed that 1.0% taurine supplementation in diets incorporating high levels of soy products might have a pronounced effect on flesh quality of European sea bass. |
2018 |
Vardali, S C; Samanidou, V F; Kotzamanis, Y P Rapid confirmatory method for the determination of danofloxacin and N-desmethyl danofloxacin in european seabass by UPLC-PDA Journal Article Current Analytical Chemistry, 14 (1), pp. 68–74, 2018, ISSN: 15734110, (Publisher: Bentham Science Publishers B.V.). @article{vardali_rapid_2018, title = {Rapid confirmatory method for the determination of danofloxacin and N-desmethyl danofloxacin in european seabass by UPLC-PDA}, author = {S C Vardali and V F Samanidou and Y P Kotzamanis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041502011&doi=10.2174%2f1573411012666160614081139&partnerID=40&md5=d3ffa1e0f520245fc4549367cd1ef36f}, doi = {10.2174/1573411012666160614081139}, issn = {15734110}, year = {2018}, date = {2018-01-01}, journal = {Current Analytical Chemistry}, volume = {14}, number = {1}, pages = {68--74}, abstract = {Background: Danofloxacin is a synthetic antibacterial agent with broad spectrum antibacterial and antimycoplasmal activity which is widely used in aquaculture. Methods: A UPLC (Ultra Performance Liquid Chromatography) method was developed and validated for the determination of danofloxacin (Dano) and its major metabolite N-desmethyl danofloxacin (Ndesmethyl Dano) in muscle plus skin tissue of European seabass (Dicentrarchus labrax) for the first time. For the separation an analytical column UPLC BEH C18 2.1 × 5 mm (1.7 µm) was used with an isocratic mobile phase consisting of MeOH-water acidified with formic acid (0.01%) (18:82; v/v) and flow rate of 0.3 mL/min within 5.5 min. The detection was performed at 275 nm using a photodiode array detector for both compounds. Examined fluoroquinolones were isolated from seabass muscle and skin tissue by extraction with acidic ACN and further purification with the QuEChERS methodology. Results: Recovery rates from muscle and skin tissue ranged between 90.2 and 101.2% for both compounds. The detection limit of the method was estimated at 13.73 µg/kg for Dano, 18.32 µg/kg for Ndesmethyl Dano, while the limits of quantification were 41.62 and 55.52 µg/kg, respectively. The developed method was fully validated in terms of selectivity, linearity, accuracy, precision, stability and sensitivity according to the European Union Decision 2002/657/EC. © 2018 Bentham Science Publishers.}, note = {Publisher: Bentham Science Publishers B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Background: Danofloxacin is a synthetic antibacterial agent with broad spectrum antibacterial and antimycoplasmal activity which is widely used in aquaculture. Methods: A UPLC (Ultra Performance Liquid Chromatography) method was developed and validated for the determination of danofloxacin (Dano) and its major metabolite N-desmethyl danofloxacin (Ndesmethyl Dano) in muscle plus skin tissue of European seabass (Dicentrarchus labrax) for the first time. For the separation an analytical column UPLC BEH C18 2.1 × 5 mm (1.7 µm) was used with an isocratic mobile phase consisting of MeOH-water acidified with formic acid (0.01%) (18:82; v/v) and flow rate of 0.3 mL/min within 5.5 min. The detection was performed at 275 nm using a photodiode array detector for both compounds. Examined fluoroquinolones were isolated from seabass muscle and skin tissue by extraction with acidic ACN and further purification with the QuEChERS methodology. Results: Recovery rates from muscle and skin tissue ranged between 90.2 and 101.2% for both compounds. The detection limit of the method was estimated at 13.73 µg/kg for Dano, 18.32 µg/kg for Ndesmethyl Dano, while the limits of quantification were 41.62 and 55.52 µg/kg, respectively. The developed method was fully validated in terms of selectivity, linearity, accuracy, precision, stability and sensitivity according to the European Union Decision 2002/657/EC. © 2018 Bentham Science Publishers. |
Vardali, S C; Samanidou, V F; Kotzamanis, Y P Journal of Chromatography A, 1575 , pp. 40–48, 2018, ISSN: 00219673, (Publisher: Elsevier B.V.). @article{vardali_development_2018, title = {Development and validation of an ultra performance liquid chromatography-quadrupole time of flight-mass spectrometry (in MSE mode) method for the quantitative determination of 20 antimicrobial residues in edible muscle tissue of European sea bass}, author = {S C Vardali and V F Samanidou and Y P Kotzamanis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85053198841&doi=10.1016%2fj.chroma.2018.09.017&partnerID=40&md5=edc0011bae4f941c97c541fd3c3410cf}, doi = {10.1016/j.chroma.2018.09.017}, issn = {00219673}, year = {2018}, date = {2018-01-01}, journal = {Journal of Chromatography A}, volume = {1575}, pages = {40--48}, abstract = {A UPLC-QTOF-MS method for the simultaneous determination of 20 veterinary drug residues and metabolites (tetracyclines, quinolones, sulfonamides and diaminopyrimidines) in edible muscle plus skin tissue of European sea bass (Dicentrarchus Labrax) was developed. For the identification of analytes a positive electrospray ionization quadropole time-of flight mass spectrometer operating in MSE mode (UPLC-QTOF-MSE) was used. MSE mode provides high chromatographic resolution and accurate mass measurements in both MS and MS/MS modes simultaneously in a single run. Separation was achieved on a UPLC BEH C18 (50 mm × 2.1 mm, 1.7 μm) column in a gradient elution program of 10 min. Examined antibiotics were isolated easily after a simple solid-liquid extraction procedure with acidic acetonitrile (0.1% v/v formic acid) and Na2EDTA 0.1 M. Recovery rates from muscle plus skin tissue ranged from 93.8% to 107.5% for all targeted compounds. The detection limits and the limits of quantification ranged from 2.22 to 15.00 μg/kg, and from 6.67 to 45.46 μg/kg, respectively. The developed method was validated in terms of selectivity, matrix effect, linearity, accuracy, precision, stability and sensitivity, CCα and CCβ according to European Union Decision 2002/657/EC. The proposed method was applied for the analysis of contaminated fish samples after in feed administration of danofloxacin mesylate. © 2018 Elsevier B.V.}, note = {Publisher: Elsevier B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A UPLC-QTOF-MS method for the simultaneous determination of 20 veterinary drug residues and metabolites (tetracyclines, quinolones, sulfonamides and diaminopyrimidines) in edible muscle plus skin tissue of European sea bass (Dicentrarchus Labrax) was developed. For the identification of analytes a positive electrospray ionization quadropole time-of flight mass spectrometer operating in MSE mode (UPLC-QTOF-MSE) was used. MSE mode provides high chromatographic resolution and accurate mass measurements in both MS and MS/MS modes simultaneously in a single run. Separation was achieved on a UPLC BEH C18 (50 mm × 2.1 mm, 1.7 μm) column in a gradient elution program of 10 min. Examined antibiotics were isolated easily after a simple solid-liquid extraction procedure with acidic acetonitrile (0.1% v/v formic acid) and Na2EDTA 0.1 M. Recovery rates from muscle plus skin tissue ranged from 93.8% to 107.5% for all targeted compounds. The detection limits and the limits of quantification ranged from 2.22 to 15.00 μg/kg, and from 6.67 to 45.46 μg/kg, respectively. The developed method was validated in terms of selectivity, matrix effect, linearity, accuracy, precision, stability and sensitivity, CCα and CCβ according to European Union Decision 2002/657/EC. The proposed method was applied for the analysis of contaminated fish samples after in feed administration of danofloxacin mesylate. © 2018 Elsevier B.V. |
Kotzamanis, Y; Kouroupakis, E; Ilia, V; Haralabous, J; Papaioannou, N; Papanna, K; Richards, R; Gisbert, E Aquaculture Nutrition, 24 (6), pp. 1738–1751, 2018, ISSN: 13535773, (Publisher: Blackwell Publishing Ltd). @article{kotzamanis_effects_2018, title = {Effects of high-level fishmeal replacement by plant proteins supplemented with different levels of lysine on growth performance and incidence of systemic noninfectious granulomatosis in meagre (Argyrosomus regius)}, author = {Y Kotzamanis and E Kouroupakis and V Ilia and J Haralabous and N Papaioannou and K Papanna and R Richards and E Gisbert}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85056334799&doi=10.1111%2fanu.12814&partnerID=40&md5=802b0a99ecb9ce48331ab78ef8d30e87}, doi = {10.1111/anu.12814}, issn = {13535773}, year = {2018}, date = {2018-01-01}, journal = {Aquaculture Nutrition}, volume = {24}, number = {6}, pages = {1738--1751}, abstract = {The potential use of plant protein (PP) blends (soybean, wheat, rapeseed, corn gluten and wheat gluten) in the diet of juvenile meagre (Argyrosomus regius) was investigated at increasing levels to replace fishmeal (FM) (33% and 56%) in six isonitrogenous (480 g/kg) and isoenergetic (22 MJ/kg) diets, which were supplemented with crystalline lysine. Meagre juveniles (36 ± 0.6 g initial weight) were reared in triplicate for 60 days at 19.4 ± 2.4°C to evaluate their growth performance, feed utilization parameters, body proximate composition and the prevalence of systemic noninfectious granulomatosis. Results indicated that there was no significant difference (GLM ANOVA, p textgreater 0.05) in growth performance and feed utilization parameters in meagre fed the diet containing 300 g/kg FM (33% FM replacement) compared to the control group (450 g/kg FM inclusion), although a trend showing inferior body gain and feed conversion ratio was observed. However, higher levels of FM replacement (56%) by PP blends (200 g/kg FM inclusion) significantly impaired growth performance, feed conversion and protein efficiency rates (p textless 0.05), which may be linked to a decrease in feed intake and/or reduced levels of bioactive compounds or other micronutrients present in FM. On the other hand, increasing dietary lysine levels from 25 to 29 g/kg in the diets containing the same PP content and 200 g/kg inclusion of FM significantly improved growth performance in juvenile meagre. The replacement of FM did not affect lipidosomatic and hepatosomatic indexes in any of the experimental groups evaluated (p textgreater 0.05). The aetiology of granulomatosis found in different tissues was not due to the presence of bacteria, as no bacterial structures were detected in histological slides when samples were stained with the Gram, Ziehl-Neelsen and Fite-Faraco staining. The presence of chronic systemic noninfectious granulomatosis was observed in meagre from all the experimental groups regardless the level of FM replacement by PP blends, indicating that the onset and progression of granulomatosis occurred insidiously at earlier life stages of meagre and persisted at variable levels thereafter. The liver and kidney were found to be the most severely affected tissues. © 2018 John Wiley & Sons Ltd}, note = {Publisher: Blackwell Publishing Ltd}, keywords = {}, pubstate = {published}, tppubtype = {article} } The potential use of plant protein (PP) blends (soybean, wheat, rapeseed, corn gluten and wheat gluten) in the diet of juvenile meagre (Argyrosomus regius) was investigated at increasing levels to replace fishmeal (FM) (33% and 56%) in six isonitrogenous (480 g/kg) and isoenergetic (22 MJ/kg) diets, which were supplemented with crystalline lysine. Meagre juveniles (36 ± 0.6 g initial weight) were reared in triplicate for 60 days at 19.4 ± 2.4°C to evaluate their growth performance, feed utilization parameters, body proximate composition and the prevalence of systemic noninfectious granulomatosis. Results indicated that there was no significant difference (GLM ANOVA, p textgreater 0.05) in growth performance and feed utilization parameters in meagre fed the diet containing 300 g/kg FM (33% FM replacement) compared to the control group (450 g/kg FM inclusion), although a trend showing inferior body gain and feed conversion ratio was observed. However, higher levels of FM replacement (56%) by PP blends (200 g/kg FM inclusion) significantly impaired growth performance, feed conversion and protein efficiency rates (p textless 0.05), which may be linked to a decrease in feed intake and/or reduced levels of bioactive compounds or other micronutrients present in FM. On the other hand, increasing dietary lysine levels from 25 to 29 g/kg in the diets containing the same PP content and 200 g/kg inclusion of FM significantly improved growth performance in juvenile meagre. The replacement of FM did not affect lipidosomatic and hepatosomatic indexes in any of the experimental groups evaluated (p textgreater 0.05). The aetiology of granulomatosis found in different tissues was not due to the presence of bacteria, as no bacterial structures were detected in histological slides when samples were stained with the Gram, Ziehl-Neelsen and Fite-Faraco staining. The presence of chronic systemic noninfectious granulomatosis was observed in meagre from all the experimental groups regardless the level of FM replacement by PP blends, indicating that the onset and progression of granulomatosis occurred insidiously at earlier life stages of meagre and persisted at variable levels thereafter. The liver and kidney were found to be the most severely affected tissues. © 2018 John Wiley & Sons Ltd |
2017 |
Vardali, S C; Kotzamanis, Y P; Tyrpenou, A E; Samanidοu, V F Aquaculture, 479 , pp. 538–543, 2017, ISSN: 00448486, (Publisher: Elsevier B.V.). @article{vardali_danofloxacin_2017, title = {Danofloxacin depletion from muscle plus skin tissue of European sea bass (Dicentrarchus labrax) fed danofloxacin mesylate medicated feed in seawater at 16 °C and 27 °C}, author = {S C Vardali and Y P Kotzamanis and A E Tyrpenou and V F Samanidοu}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021677464&doi=10.1016%2fj.aquaculture.2017.06.036&partnerID=40&md5=0f972570d7da39528df9aeca748124c0}, doi = {10.1016/j.aquaculture.2017.06.036}, issn = {00448486}, year = {2017}, date = {2017-01-01}, journal = {Aquaculture}, volume = {479}, pages = {538--543}, abstract = {Residue depletion of danofloxacin was investigated in European sea bass after a multiple (5 days) in-feed administration of danofloxacin mesylate (10 mg/kg bw per day) under experimental field conditions at water temperature of 16 °C and 27 °C. Ten fish per sampling point were examined during and after treatment. Samples of muscle plus skin in natural proportions were analyzed for danofloxacin determination by Ultra-Performance Liquid Chromatography equipped with an Ultraviolet Photodiode Array Detector (UPLC-PDA). Danofloxacin concentrations in muscle tissues increased during medication period and then decreased rapidly. The elimination half-lives were estimated to be t1/2 = 16.87 h and 21.13 h at 27 °C and 16 °C, respectively. Withdrawal periods (Wt) were calculated after medication using the WT1.4 program and were found to be at 95% tolerance limit, 7 and 4 days at 16 °C and 27 °C, respectively. © 2017 Elsevier B.V.}, note = {Publisher: Elsevier B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Residue depletion of danofloxacin was investigated in European sea bass after a multiple (5 days) in-feed administration of danofloxacin mesylate (10 mg/kg bw per day) under experimental field conditions at water temperature of 16 °C and 27 °C. Ten fish per sampling point were examined during and after treatment. Samples of muscle plus skin in natural proportions were analyzed for danofloxacin determination by Ultra-Performance Liquid Chromatography equipped with an Ultraviolet Photodiode Array Detector (UPLC-PDA). Danofloxacin concentrations in muscle tissues increased during medication period and then decreased rapidly. The elimination half-lives were estimated to be t1/2 = 16.87 h and 21.13 h at 27 °C and 16 °C, respectively. Withdrawal periods (Wt) were calculated after medication using the WT1.4 program and were found to be at 95% tolerance limit, 7 and 4 days at 16 °C and 27 °C, respectively. © 2017 Elsevier B.V. |
2016 |
Gisbert, E; Mozanzadeh, M T; Kotzamanis, Y; Estévez, A Weaning wild flathead grey mullet (Mugil cephalus) fry with diets with different levels of fish meal substitution Journal Article Aquaculture, 462 , pp. 92–100, 2016, ISSN: 00448486, (Publisher: Elsevier B.V.). @article{gisbert_weaning_2016, title = {Weaning wild flathead grey mullet (Mugil cephalus) fry with diets with different levels of fish meal substitution}, author = {E Gisbert and M T Mozanzadeh and Y Kotzamanis and A Estévez}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84971668055&doi=10.1016%2fj.aquaculture.2016.04.035&partnerID=40&md5=a634fa157033cece66c715fb5fe3a5e4}, doi = {10.1016/j.aquaculture.2016.04.035}, issn = {00448486}, year = {2016}, date = {2016-01-01}, journal = {Aquaculture}, volume = {462}, pages = {92--100}, abstract = {The culture of flathead grey mullet (Mugil cephalus) is based on wild fry captured during their migration into estuarine environments and consequently, optimizing weaning diets is of special importance for this species at this particular stage of development. Thus, authors have tested a weaning protocol for wild flathead grey mullet fry (202 mg initial body weight) during 60 days (18.1 ± 0.3 °C, salinity, 1.2 ± 0.2‰) using compound diets (36% crude protein, 16% crude fat) with different levels of fish meal (FM) substitution by plant protein (PP) sources (50% and 75% of FM replacement by a blend of corn gluten, wheat gluten, soy bean meal and soy protein concentrate). Fry were progressively weaned onto experimental diets during the first 20 days of the trial (25% Artemia metanauplii replacement each 5 days), whereas compound diets were offered at a feed ratio of 5% of stocked biomass until the end of the trial (day 60). A blend of PP sources (corn gluten, wheat gluten and soy protein concentrate) as the main dietary protein sources in combination with crystalline l-lysine and dl-methionine dietary supplementation were as good as a FM-based diet in terms of growth performance, digestive physiology and fish condition (i.e. proximate composition, oxidative stress status). As the cost-benefit proxy analysis of the tested weaning diets indicated, the inclusion of alternative PP was a satisfactory strategy in terms of feed price reduction, since PP50 and PP75 diets were 15.5 and 23.6% cheaper than the FM diet. Although wild flathead grey mullet fry were satisfactorily weaned onto diets containing 75% FM substitution by PP sources, present results indicated that complete FM replacement in weaning diets for this species might also be feasible. Statement of relevance: In this study, authors have tested a weaning protocol for wild flathead grey mullet (Mugil cephalus) fry based on diets with different levels of fish meal substitution by alternative plant protein sources. This is of special importance due to the importance of the aquaculture of this species in several regions of the word, as well as for the use of wild animals for on-growing purposes. Results showed that diets with 75% of fish meal substitution can be successfully used for weaning and on-growing wild fry without any detrimental effect of fry performance and condition. © 2016 Elsevier B.V.}, note = {Publisher: Elsevier B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The culture of flathead grey mullet (Mugil cephalus) is based on wild fry captured during their migration into estuarine environments and consequently, optimizing weaning diets is of special importance for this species at this particular stage of development. Thus, authors have tested a weaning protocol for wild flathead grey mullet fry (202 mg initial body weight) during 60 days (18.1 ± 0.3 °C, salinity, 1.2 ± 0.2‰) using compound diets (36% crude protein, 16% crude fat) with different levels of fish meal (FM) substitution by plant protein (PP) sources (50% and 75% of FM replacement by a blend of corn gluten, wheat gluten, soy bean meal and soy protein concentrate). Fry were progressively weaned onto experimental diets during the first 20 days of the trial (25% Artemia metanauplii replacement each 5 days), whereas compound diets were offered at a feed ratio of 5% of stocked biomass until the end of the trial (day 60). A blend of PP sources (corn gluten, wheat gluten and soy protein concentrate) as the main dietary protein sources in combination with crystalline l-lysine and dl-methionine dietary supplementation were as good as a FM-based diet in terms of growth performance, digestive physiology and fish condition (i.e. proximate composition, oxidative stress status). As the cost-benefit proxy analysis of the tested weaning diets indicated, the inclusion of alternative PP was a satisfactory strategy in terms of feed price reduction, since PP50 and PP75 diets were 15.5 and 23.6% cheaper than the FM diet. Although wild flathead grey mullet fry were satisfactorily weaned onto diets containing 75% FM substitution by PP sources, present results indicated that complete FM replacement in weaning diets for this species might also be feasible. Statement of relevance: In this study, authors have tested a weaning protocol for wild flathead grey mullet (Mugil cephalus) fry based on diets with different levels of fish meal substitution by alternative plant protein sources. This is of special importance due to the importance of the aquaculture of this species in several regions of the word, as well as for the use of wild animals for on-growing purposes. Results showed that diets with 75% of fish meal substitution can be successfully used for weaning and on-growing wild fry without any detrimental effect of fry performance and condition. © 2016 Elsevier B.V. |
2014 |
Feidantsis, K; Kaitetzidou, E; Mavrogiannis, N; Michaelidis, B; Kotzamanis, Y; Antonopoulou, E Aquaculture Nutrition, 20 (4), pp. 431–442, 2014, ISSN: 13535773, (Publisher: Blackwell Publishing Ltd). @article{feidantsis_effect_2014, title = {Effect of taurine-enriched diets on the Hsp expression, MAPK activation and the antioxidant defence of the European sea bass (Dicentrarchus labrax)}, author = {K Feidantsis and E Kaitetzidou and N Mavrogiannis and B Michaelidis and Y Kotzamanis and E Antonopoulou}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84903899185&doi=10.1111%2fanu.12096&partnerID=40&md5=33717104f36c4f17b28f24bd6fd1eb7a}, doi = {10.1111/anu.12096}, issn = {13535773}, year = {2014}, date = {2014-01-01}, journal = {Aquaculture Nutrition}, volume = {20}, number = {4}, pages = {431--442}, abstract = {This study aimed to identify and characterize the cellular and metabolic response in vital organs of Dicentrarchus labrax, after feeding on soy-based diets enriched with different taurine concentrations (T0.2, T0.5, T1.0 w/w). Molecular responses (expression of Hsp70, Hsp90, phosphorylation of p38 mitogen-activated protein kinase-p38MAPK and extracellular signal-regulated kinases-ERK1/2) were addressed. Metabolic and antioxidant capacities such as activities of citrate synthase (CS), malate and lactate dehydrogenase (MDH, L-LDH), superoxide dismutase (SOD), catalase (CAT) and levels of thiobarbituric acid-reactive substances (TBARS) were assessed. The most intense changes were detected in the liver and in the anterior intestine showing that taurine induces important protective biochemical mechanisms. Specifically, in the liver and the anterior intestine, Hsp and MAPK levels, as well as L-LDH activity levels were up-regulated under the effect of taurine diets. T diet had no major effect on the activity levels of CS, SOD and TBARS, except for MDH in the liver and catalase in the anterior intestine, where activity levels of these enzymes were increased under the effect of T0.2 and T1.0, respectively. These results may facilitate monitoring of adequate health status of farmed fish, exploiting the beneficial T effects and could assist at the formulation of aquaculture feeding strategies. © 2014 John Wiley & Sons Ltd.}, note = {Publisher: Blackwell Publishing Ltd}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study aimed to identify and characterize the cellular and metabolic response in vital organs of Dicentrarchus labrax, after feeding on soy-based diets enriched with different taurine concentrations (T0.2, T0.5, T1.0 w/w). Molecular responses (expression of Hsp70, Hsp90, phosphorylation of p38 mitogen-activated protein kinase-p38MAPK and extracellular signal-regulated kinases-ERK1/2) were addressed. Metabolic and antioxidant capacities such as activities of citrate synthase (CS), malate and lactate dehydrogenase (MDH, L-LDH), superoxide dismutase (SOD), catalase (CAT) and levels of thiobarbituric acid-reactive substances (TBARS) were assessed. The most intense changes were detected in the liver and in the anterior intestine showing that taurine induces important protective biochemical mechanisms. Specifically, in the liver and the anterior intestine, Hsp and MAPK levels, as well as L-LDH activity levels were up-regulated under the effect of taurine diets. T diet had no major effect on the activity levels of CS, SOD and TBARS, except for MDH in the liver and catalase in the anterior intestine, where activity levels of these enzymes were increased under the effect of T0.2 and T1.0, respectively. These results may facilitate monitoring of adequate health status of farmed fish, exploiting the beneficial T effects and could assist at the formulation of aquaculture feeding strategies. © 2014 John Wiley & Sons Ltd. |
Skalli, A; Zambonino-Infante, J -L; Kotzamanis, Y; Fabregat, R; Gisbert, E Aquaculture Nutrition, 20 (2), pp. 118–131, 2014, ISSN: 13535773, (Publisher: Blackwell Publishing Ltd). @article{skalli_peptide_2014, title = {Peptide molecular weight distribution of soluble protein fraction affects growth performance and quality in European sea bass (Dicentrarchus labrax) larvae}, author = {A Skalli and J -L Zambonino-Infante and Y Kotzamanis and R Fabregat and E Gisbert}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84896316449&doi=10.1111%2fanu.12058&partnerID=40&md5=8ebbe6336746885c8515331ce9e2bf14}, doi = {10.1111/anu.12058}, issn = {13535773}, year = {2014}, date = {2014-01-01}, journal = {Aquaculture Nutrition}, volume = {20}, number = {2}, pages = {118--131}, abstract = {Dicentrarchus labrax were fed from 10 to 40 days posthatching with six microdiets differing in the inclusion level (60 g kg-1 and 120 g kg-1) and type of protein hydrolysate (PH; yeast, YPH; pig blood, PBPH; pig red blood cells, PRBCPH). A microdiet containing 120 g kg-1 fish PH (FPH) was used as a control. PH differed in their amino acid (AA) profile and molecular weight distribution and therefore the tested microdiets too. The estimated content in FAA and di- and tripeptides in the FPH microdiet was 2 g kg-1 and 44 g kg-1, respectively. FAA estimated levels in YPH and PBPH microdiets were 26 g kg-1 and 53 g kg-1, whereas levels of di- and tripeptides were 30 g kg-1 and 60 g kg-1, respectively. The estimated levels of FAA in PRBCPH microdiets were 8 g kg-1 and 17 g kg-1, whereas estimated levels of di- and tripeptides were 11 g kg-1 and 22 g kg-1, respectively. Results revealed that FPH may be replaced by alternative PH from yeast and pig blood products, as fish fed those diets performed, in terms of growth, survival, digestive function and incidence of skeletal deformities, as well as those fed the FPH microdiet. Using YPH, PBPH and PRBCPH, the inclusion level of PH in microdiets might be reduced to a half with respect to current practices using FPH. © 2013 John Wiley & Sons Ltd.}, note = {Publisher: Blackwell Publishing Ltd}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dicentrarchus labrax were fed from 10 to 40 days posthatching with six microdiets differing in the inclusion level (60 g kg-1 and 120 g kg-1) and type of protein hydrolysate (PH; yeast, YPH; pig blood, PBPH; pig red blood cells, PRBCPH). A microdiet containing 120 g kg-1 fish PH (FPH) was used as a control. PH differed in their amino acid (AA) profile and molecular weight distribution and therefore the tested microdiets too. The estimated content in FAA and di- and tripeptides in the FPH microdiet was 2 g kg-1 and 44 g kg-1, respectively. FAA estimated levels in YPH and PBPH microdiets were 26 g kg-1 and 53 g kg-1, whereas levels of di- and tripeptides were 30 g kg-1 and 60 g kg-1, respectively. The estimated levels of FAA in PRBCPH microdiets were 8 g kg-1 and 17 g kg-1, whereas estimated levels of di- and tripeptides were 11 g kg-1 and 22 g kg-1, respectively. Results revealed that FPH may be replaced by alternative PH from yeast and pig blood products, as fish fed those diets performed, in terms of growth, survival, digestive function and incidence of skeletal deformities, as well as those fed the FPH microdiet. Using YPH, PBPH and PRBCPH, the inclusion level of PH in microdiets might be reduced to a half with respect to current practices using FPH. © 2013 John Wiley & Sons Ltd. |
2012 |
Kotzamanis, Y Invited chairman at the ‘Larval Nutrition session’ Inproceedings Kotzamanis, Y (Ed.): 15th International Symposium on Fish Nutrition and Feeding, Molde, Norway, Molde, Norway, 2012. @inproceedings{kotzamanis_invited_2012, title = {Invited chairman at the ‘Larval Nutrition session’}, author = {Y Kotzamanis}, editor = {Y Kotzamanis}, year = {2012}, date = {2012-06-01}, booktitle = {15th International Symposium on Fish Nutrition and Feeding, Molde, Norway}, address = {Molde, Norway}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Gisbert, E; Skalli, A; Fernández, I; Kotzamanis, Y; Zambonino-Infante, J L; Fabregat, R Protein hydrolysates from yeast and pig blood as alternative raw materials in microdiets for gilthead sea bream (Sparus aurata) larvae Journal Article Aquaculture, 338-341 , pp. 96–104, 2012, ISSN: 00448486. @article{gisbert_protein_2012, title = {Protein hydrolysates from yeast and pig blood as alternative raw materials in microdiets for gilthead sea bream (Sparus aurata) larvae}, author = {E Gisbert and A Skalli and I Fernández and Y Kotzamanis and J L Zambonino-Infante and R Fabregat}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84858335976&doi=10.1016%2fj.aquaculture.2012.01.007&partnerID=40&md5=7a565e32abef6da7fdaeeb2a80550040}, doi = {10.1016/j.aquaculture.2012.01.007}, issn = {00448486}, year = {2012}, date = {2012-01-01}, journal = {Aquaculture}, volume = {338-341}, pages = {96--104}, abstract = {In this study, we have evaluated the incorporation of two types of protein hydrolysates at 9 and 12% levels of inclusion, one from yeast (Saccharomyces cerevisiae, YPH) and another one from pig blood (PBPH), in microdiets for gilthead sea bream (Sparus aurata) larvae, and compared these results to a microdiet containing fish protein hydrolysate and another group only fed with enriched live prey (rotifers and Artemia). The trial consisted in substituting up to 75% (wt/wt) the enriched Artemia with the experimental microdiets from 15 to 40. days post-hatch, whereas larvae were exclusively fed on microdiets from 40 to 55. dph. Protein hydrolysates used in the present study were obtained from different raw materials (yeast, pig blood and fish protein concentrate) and differed in their amino acid (AA) profile and in their molecular weight distribution. YPH and PBPH were mainly composed by free amino acids (FAA) (44%, MW textless 200 Da), di- and tripeptides (50%, 200 textless MW textless 500 Da) and 6% of larger polypeptides (500 textless MW textless 2500 Da); whereas the fish protein hydrolysate (FPH) did only contain a minor quantity of FAA (1.5%) and was mainly composed of di- and tripeptides (36.5%) and larger polypeptides (51.4%, 500 textless MW textless 2500. Da). The contents in FAA and di- and tripeptides in the microdiet containing FPH were 0.2 and 4.4%, respectively. FAA levels in microdiets including YPH and PBPH at 9 and 12% were 4.0 and 5.3%, whereas levels of di- and tripeptides were 4.5 and 6.0%, respectively. Results revealed that FPH in microdiets for marine fish larvae may be replaced by alternative protein hydrolysates obtained from yeast and pig blood, as fish fed with those diets performed, in terms of growth, survival, level of maturation of the enterocytes (activity of cytosolic and brush border enzymes) and incidence of skeletal deformities, as well as those larvae fed with only enriched live preys (rotifers and Artemia). Using YPH and PBPH, the inclusion level of protein hydrolysate in microdiets might be reduced to 9% (3% lesser to actual practices using fish protein hydrolysates) without affecting larval performance. Present results suggested the importance of leucine, valine and phenylalanine in fish larval skeletogenesis and in the appearance of skeletal disorders. © 2012 Elsevier B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In this study, we have evaluated the incorporation of two types of protein hydrolysates at 9 and 12% levels of inclusion, one from yeast (Saccharomyces cerevisiae, YPH) and another one from pig blood (PBPH), in microdiets for gilthead sea bream (Sparus aurata) larvae, and compared these results to a microdiet containing fish protein hydrolysate and another group only fed with enriched live prey (rotifers and Artemia). The trial consisted in substituting up to 75% (wt/wt) the enriched Artemia with the experimental microdiets from 15 to 40. days post-hatch, whereas larvae were exclusively fed on microdiets from 40 to 55. dph. Protein hydrolysates used in the present study were obtained from different raw materials (yeast, pig blood and fish protein concentrate) and differed in their amino acid (AA) profile and in their molecular weight distribution. YPH and PBPH were mainly composed by free amino acids (FAA) (44%, MW textless 200 Da), di- and tripeptides (50%, 200 textless MW textless 500 Da) and 6% of larger polypeptides (500 textless MW textless 2500 Da); whereas the fish protein hydrolysate (FPH) did only contain a minor quantity of FAA (1.5%) and was mainly composed of di- and tripeptides (36.5%) and larger polypeptides (51.4%, 500 textless MW textless 2500. Da). The contents in FAA and di- and tripeptides in the microdiet containing FPH were 0.2 and 4.4%, respectively. FAA levels in microdiets including YPH and PBPH at 9 and 12% were 4.0 and 5.3%, whereas levels of di- and tripeptides were 4.5 and 6.0%, respectively. Results revealed that FPH in microdiets for marine fish larvae may be replaced by alternative protein hydrolysates obtained from yeast and pig blood, as fish fed with those diets performed, in terms of growth, survival, level of maturation of the enterocytes (activity of cytosolic and brush border enzymes) and incidence of skeletal deformities, as well as those larvae fed with only enriched live preys (rotifers and Artemia). Using YPH and PBPH, the inclusion level of protein hydrolysate in microdiets might be reduced to 9% (3% lesser to actual practices using fish protein hydrolysates) without affecting larval performance. Present results suggested the importance of leucine, valine and phenylalanine in fish larval skeletogenesis and in the appearance of skeletal disorders. © 2012 Elsevier B.V. |
Slawski, H; Adem, H; Tressel, R -P; Wysujack, K; Koops, U; Kotzamanis, Y; Wuertz, S; Schulz, C Total fish meal replacement with rapeseed protein concentrate in diets fed to rainbow trout (Oncorhynchus mykiss Walbaum) Journal Article Aquaculture International, 20 (3), pp. 443–453, 2012, ISSN: 09676120. @article{slawski_total_2012, title = {Total fish meal replacement with rapeseed protein concentrate in diets fed to rainbow trout (Oncorhynchus mykiss Walbaum)}, author = {H Slawski and H Adem and R -P Tressel and K Wysujack and U Koops and Y Kotzamanis and S Wuertz and C Schulz}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84860881834&doi=10.1007%2fs10499-011-9476-2&partnerID=40&md5=1728dc27fd870033083cf9ddb66fefb6}, doi = {10.1007/s10499-011-9476-2}, issn = {09676120}, year = {2012}, date = {2012-01-01}, journal = {Aquaculture International}, volume = {20}, number = {3}, pages = {443--453}, abstract = {The potential of rapeseed protein concentrate as fish meal alternative in diets for rainbow trout (initial average weight 37.8 ± 1.4 g) was evaluated. Nine experimental tanks of a freshwater flow-through system were stocked with 12 fish each. Triplicate groups of fish received isonitrogenous (47.9 ± 0.5% CP) and isoenergetic (22.4 ± 0.2 kJ g-1) experimental diets with 0, 66 and 100% of fish meal substituted with rapeseed protein concentrate (71.2% CP), thereby providing 0, 29 and 43% of dietary protein. As the amino acid profile of rapeseed protein concentrate was comparable to fish meal, there was no need to supplement experimental diets with synthetic amino acids. At the end of the 84 days of feeding period, fish growth performance, feed intake and feed efficiencies were not compromised, when 100% of fish meal in the control diet was replaced with rapeseed protein concentrate, revealing a SGR of 1.19 or 1.10, a FCR of 1.09 or 1.18 and a feed intake of 78.5 or 74.7 g in fish fed on the control diet or fed the diet devoid of fish meal, respectively. Intestinal morphology did not reveal any histological abnormalities in all dietary groups. Blood parameters including haematocrit and haemoglobin as well as glucose, triglycerides and total protein in the plasma were not different between treatment groups. Thus, the rapeseed protein concentrate tested here has great potential as an alternative to fish meal in rainbow trout diets. © 2011 Springer Science+Business Media B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The potential of rapeseed protein concentrate as fish meal alternative in diets for rainbow trout (initial average weight 37.8 ± 1.4 g) was evaluated. Nine experimental tanks of a freshwater flow-through system were stocked with 12 fish each. Triplicate groups of fish received isonitrogenous (47.9 ± 0.5% CP) and isoenergetic (22.4 ± 0.2 kJ g-1) experimental diets with 0, 66 and 100% of fish meal substituted with rapeseed protein concentrate (71.2% CP), thereby providing 0, 29 and 43% of dietary protein. As the amino acid profile of rapeseed protein concentrate was comparable to fish meal, there was no need to supplement experimental diets with synthetic amino acids. At the end of the 84 days of feeding period, fish growth performance, feed intake and feed efficiencies were not compromised, when 100% of fish meal in the control diet was replaced with rapeseed protein concentrate, revealing a SGR of 1.19 or 1.10, a FCR of 1.09 or 1.18 and a feed intake of 78.5 or 74.7 g in fish fed on the control diet or fed the diet devoid of fish meal, respectively. Intestinal morphology did not reveal any histological abnormalities in all dietary groups. Blood parameters including haematocrit and haemoglobin as well as glucose, triglycerides and total protein in the plasma were not different between treatment groups. Thus, the rapeseed protein concentrate tested here has great potential as an alternative to fish meal in rainbow trout diets. © 2011 Springer Science+Business Media B.V. |
2011 |
Estévez, A; Treviño, L; Kotzamanis, Y; Karacostas, I; Tort, L; Gisbert, E Effects of different levels of plant proteins on the ongrowing of meagre (Argyrosomus regius) juveniles at low temperatures Journal Article Aquaculture Nutrition, 17 (2), pp. e572–e582, 2011, ISSN: 13535773. @article{estevez_effects_2011, title = {Effects of different levels of plant proteins on the ongrowing of meagre (Argyrosomus regius) juveniles at low temperatures}, author = {A Estévez and L Treviño and Y Kotzamanis and I Karacostas and L Tort and E Gisbert}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79952517035&doi=10.1111%2fj.1365-2095.2010.00798.x&partnerID=40&md5=f0769fb16f2511d31d08e9e99336b51d}, doi = {10.1111/j.1365-2095.2010.00798.x}, issn = {13535773}, year = {2011}, date = {2011-01-01}, journal = {Aquaculture Nutrition}, volume = {17}, number = {2}, pages = {e572--e582}, abstract = {Four experimental diets with different inclusion levels of plant proteins and fish protein hydrolysates were compared with a commercial diet for meagre (Argyrosomus regius) ongrowing at optimal and suboptimal water temperature. Results in terms of growth in length and weight, conversion efficiency, dietary feed intake and utilization, body composition (whole fish and liver) as well as enzyme and immunological activities are presented. Fish growth was significantly reduced by the inclusion of plant proteins, although further addition of fish protein hydrolysates improved the results. Daily feed intake was not affected by plant protein inclusion in the diets, although the group fed the highest inclusion level showed lower ingestion than the rest of the groups, probably as a consequence of a reduced dietary palatability. The decrease in water temperature during the second part of the experiment had a negative effect on feed intake and fish growth. Gross visceral morphology of meagre fed the experimental diets was not affected, but muscle weight was significantly reduced. Whole body and liver composition was not affected with plant protein inclusion. However, the inclusion of fish protein hydrolysates resulted in a significant increase in fat content, especially in liver cholesterol and steryl esters, with a parallel reduction in protein. Brush border enzymes were affected by plant protein inclusion as well as serum lysozyme that significantly increased in the fish fed the highest inclusion level. As a conclusion, up to 315gkg-1 plant protein (76.2% of total protein content) can be included in the diet for meagre without affecting growth or feed utilization. Higher inclusion levels can also be used if at least 5% fish protein hydrolysate is also included. © 2010 Blackwell Publishing Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Four experimental diets with different inclusion levels of plant proteins and fish protein hydrolysates were compared with a commercial diet for meagre (Argyrosomus regius) ongrowing at optimal and suboptimal water temperature. Results in terms of growth in length and weight, conversion efficiency, dietary feed intake and utilization, body composition (whole fish and liver) as well as enzyme and immunological activities are presented. Fish growth was significantly reduced by the inclusion of plant proteins, although further addition of fish protein hydrolysates improved the results. Daily feed intake was not affected by plant protein inclusion in the diets, although the group fed the highest inclusion level showed lower ingestion than the rest of the groups, probably as a consequence of a reduced dietary palatability. The decrease in water temperature during the second part of the experiment had a negative effect on feed intake and fish growth. Gross visceral morphology of meagre fed the experimental diets was not affected, but muscle weight was significantly reduced. Whole body and liver composition was not affected with plant protein inclusion. However, the inclusion of fish protein hydrolysates resulted in a significant increase in fat content, especially in liver cholesterol and steryl esters, with a parallel reduction in protein. Brush border enzymes were affected by plant protein inclusion as well as serum lysozyme that significantly increased in the fish fed the highest inclusion level. As a conclusion, up to 315gkg-1 plant protein (76.2% of total protein content) can be included in the diet for meagre without affecting growth or feed utilization. Higher inclusion levels can also be used if at least 5% fish protein hydrolysate is also included. © 2010 Blackwell Publishing Ltd. |
Slawski, H; Adem, H; Tressel, R P; Wysujack, K; Koops, U; Kotzamanis, Y; Schulz, C Replacement of fish meal with rapeseed protein concentrate in diets fed to turbot (Psetta maxima L.). Journal Article Züchtungskunde, 83 (6), pp. 451–460, 2011. @article{slawski_replacement_2011, title = {Replacement of fish meal with rapeseed protein concentrate in diets fed to turbot (Psetta maxima L.).}, author = {H Slawski and H Adem and R P Tressel and K Wysujack and U Koops and Y Kotzamanis and C Schulz}, year = {2011}, date = {2011-01-01}, journal = {Züchtungskunde}, volume = {83}, number = {6}, pages = {451--460}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2010 |
Vatsos, I N; Kotzamanis, Y; Henry, M; Angelidis, P; Alexis, M N Monitoring stress in fish by applying image analysis to their skin mucous cells Journal Article European Journal of Histochemistry, 54 (2), pp. 107–111, 2010, ISSN: 1121760X. @article{vatsos_monitoring_2010, title = {Monitoring stress in fish by applying image analysis to their skin mucous cells}, author = {I N Vatsos and Y Kotzamanis and M Henry and P Angelidis and M N Alexis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77953160103&partnerID=40&md5=e164b9bb6870e5411efbe171878b3580}, issn = {1121760X}, year = {2010}, date = {2010-01-01}, journal = {European Journal of Histochemistry}, volume = {54}, number = {2}, pages = {107--111}, abstract = {Several authors have previously demonstrated that the number of the skin mucous cells of fish is affected by many stressors. In the present study, two experiments were conducted in order to examine the effects of two common environmental conditions on the morphology of skin of sea bass and particularly on the number and diameter of skin mucous cells. In the first experiment, two groups of sea bass (mean weight 155.6±10.3 g SD) were maintained in two different concentrations of nitrate, 100 and 700 ppm respectively, for 48 h, while a third group was used as control. In the second experiment, sea bass (initial mean weight 78.9±3.1 g SD) were divided into four groups and each group was maintained in a different level of oxygen for 9 weeks. The oxygen concentration in each group was: 3.6±0.2 ppm, 4.7±0.2 ppm, 6.2±0.2 ppm and 8.2±0.2 ppm. In both experiments the effects of the two environmental factors on the morphology of the fish skin were examined histologically and a software containing a visual basic script macro, allowing quantification of the skin mucous cells, was used to analyze the skin tissue sections. Concerning the overall morphology of the skin and the diameter of the skin mucous cells, no differences were noted in both experiments (P>0.05). It was demonstrated however, that fish maintained in the lowest oxygen level and fish maintained in the highest concentration of nitrate exhibited significantly increased number of mucous cells per skin area (mm 2). There is evidence that the enumeration of the skin mucous cells of fish can be used to monitor stress in fish. © I.N. Vatsos et al., 2010 Licensee PAGEPress, Italy.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Several authors have previously demonstrated that the number of the skin mucous cells of fish is affected by many stressors. In the present study, two experiments were conducted in order to examine the effects of two common environmental conditions on the morphology of skin of sea bass and particularly on the number and diameter of skin mucous cells. In the first experiment, two groups of sea bass (mean weight 155.6±10.3 g SD) were maintained in two different concentrations of nitrate, 100 and 700 ppm respectively, for 48 h, while a third group was used as control. In the second experiment, sea bass (initial mean weight 78.9±3.1 g SD) were divided into four groups and each group was maintained in a different level of oxygen for 9 weeks. The oxygen concentration in each group was: 3.6±0.2 ppm, 4.7±0.2 ppm, 6.2±0.2 ppm and 8.2±0.2 ppm. In both experiments the effects of the two environmental factors on the morphology of the fish skin were examined histologically and a software containing a visual basic script macro, allowing quantification of the skin mucous cells, was used to analyze the skin tissue sections. Concerning the overall morphology of the skin and the diameter of the skin mucous cells, no differences were noted in both experiments (P>0.05). It was demonstrated however, that fish maintained in the lowest oxygen level and fish maintained in the highest concentration of nitrate exhibited significantly increased number of mucous cells per skin area (mm 2). There is evidence that the enumeration of the skin mucous cells of fish can be used to monitor stress in fish. © I.N. Vatsos et al., 2010 Licensee PAGEPress, Italy. |
Liu, X D; Liang, M Q; Zhang, L M; Wang, J Y; Chang, Q; L, Wang J; Kotzamanis, Y Effects of fish protein hydrolysate levels on growth performance and biological and physiological parameters in tongue sole (Cynoglossus semilaevis Günther, 1873) post larvae Journal Article Acta Hydrobiologica Sinica, 34 (2), pp. 1–8, 2010. @article{liu_effects_2010, title = {Effects of fish protein hydrolysate levels on growth performance and biological and physiological parameters in tongue sole (Cynoglossus semilaevis Günther, 1873) post larvae}, author = {X D Liu and M Q Liang and L M Zhang and J Y Wang and Q Chang and Wang J L and Y Kotzamanis}, year = {2010}, date = {2010-01-01}, journal = {Acta Hydrobiologica Sinica}, volume = {34}, number = {2}, pages = {1--8}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2009 |
Gisbert, E; Giménez, G; Fernández, I; Kotzamanis, Y; Estévez, A Development of digestive enzymes in common dentex Dentex dentex during early ontogeny Journal Article Aquaculture, 287 (3-4), pp. 381–387, 2009, ISSN: 00448486. @article{gisbert_development_2009, title = {Development of digestive enzymes in common dentex Dentex dentex during early ontogeny}, author = {E Gisbert and G Giménez and I Fernández and Y Kotzamanis and A Estévez}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-58149475982&doi=10.1016%2fj.aquaculture.2008.10.039&partnerID=40&md5=1a1f05030afff114858844b2e4d56ea3}, doi = {10.1016/j.aquaculture.2008.10.039}, issn = {00448486}, year = {2009}, date = {2009-01-01}, journal = {Aquaculture}, volume = {287}, number = {3-4}, pages = {381--387}, abstract = {The digestive physiology of common dentex was studied by assessing the activity of different pancreatic (trypsin, chymotrypsin, amylase and lipase), intestinal (alkaline phosphatase, aminopeptidase N, maltase and leuncine-alanine peptidase) and gastric (pepsin) enzymes from hatching until the juvenile stage (50 dph at 19 °C). Enzymes involved in the digestion of protein, lipid and carbohydrate were present in common dentex larvae at hatching and before the onset of exogenous feeding. The specific activity of trypsin and chymotrypsin in newly hatched larvae was higher than that of amylase and lipase, indicating the importance of these enzymes in the cleavage of yolk proteins and hatching, while after hatching, the activity of these serine proteases dramatically decreased, whereas lipase showed the opposite trend. A progressive shift in activity from alkaline to acid proteases was observed during larval development, reflecting that alkaline proteases were not longer the main digestive enzymes involved in protein digestion after the development of gastric glands and onset of acidic digestion. Lipase total activity in common dentex peaked at 35 dph and decreased after weaning. Diet change due to weaning might partially explain the decrease in lipase activity, although this change might be also indicative of a change in the nutritional requirements of this species, since the juveniles prefer diets with high protein levels than those with lower protein and higher lipid content. Regarding intestinal enzymes, leucine-alanine peptidase and alkaline phosphatase and aminopeptidase N were found in newly hatched larvae, while maltase was detected after the onset of exogenous feeding. The achievement of an efficient brush border membrane digestion takes place much earlier (6-12 dph) than in any of the studied species which might reflect the different metabolic profile and rapid growth of this species. © 2008 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The digestive physiology of common dentex was studied by assessing the activity of different pancreatic (trypsin, chymotrypsin, amylase and lipase), intestinal (alkaline phosphatase, aminopeptidase N, maltase and leuncine-alanine peptidase) and gastric (pepsin) enzymes from hatching until the juvenile stage (50 dph at 19 °C). Enzymes involved in the digestion of protein, lipid and carbohydrate were present in common dentex larvae at hatching and before the onset of exogenous feeding. The specific activity of trypsin and chymotrypsin in newly hatched larvae was higher than that of amylase and lipase, indicating the importance of these enzymes in the cleavage of yolk proteins and hatching, while after hatching, the activity of these serine proteases dramatically decreased, whereas lipase showed the opposite trend. A progressive shift in activity from alkaline to acid proteases was observed during larval development, reflecting that alkaline proteases were not longer the main digestive enzymes involved in protein digestion after the development of gastric glands and onset of acidic digestion. Lipase total activity in common dentex peaked at 35 dph and decreased after weaning. Diet change due to weaning might partially explain the decrease in lipase activity, although this change might be also indicative of a change in the nutritional requirements of this species, since the juveniles prefer diets with high protein levels than those with lower protein and higher lipid content. Regarding intestinal enzymes, leucine-alanine peptidase and alkaline phosphatase and aminopeptidase N were found in newly hatched larvae, while maltase was detected after the onset of exogenous feeding. The achievement of an efficient brush border membrane digestion takes place much earlier (6-12 dph) than in any of the studied species which might reflect the different metabolic profile and rapid growth of this species. © 2008 Elsevier B.V. All rights reserved. |
Fountoulaki, E; Vasilaki, A; Hurtado, R; Grigorakis, K; Karacostas, I; Nengas, I; Rigos, G; Kotzamanis, Y; Venou, B; Alexis, M N Aquaculture, 289 (3-4), pp. 317–326, 2009, ISSN: 00448486. @article{fountoulaki_fish_2009, title = {Fish oil substitution by vegetable oils in commercial diets for gilthead sea bream (Sparus aurata L.); effects on growth performance, flesh quality and fillet fatty acid profile. Recovery of fatty acid profiles by a fish oil finishing diet under fluctuating water temperatures}, author = {E Fountoulaki and A Vasilaki and R Hurtado and K Grigorakis and I Karacostas and I Nengas and G Rigos and Y Kotzamanis and B Venou and M N Alexis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-61649088364&doi=10.1016%2fj.aquaculture.2009.01.023&partnerID=40&md5=e3b4d18877877d60dcd12caa1fda3f45}, doi = {10.1016/j.aquaculture.2009.01.023}, issn = {00448486}, year = {2009}, date = {2009-01-01}, journal = {Aquaculture}, volume = {289}, number = {3-4}, pages = {317--326}, abstract = {The effects of long term feeding (6 months) of commercial diets with low fish meal content and high levels of vegetable oils (69% fish oil substitution level) were determined in gilthead sea bream (110 g). A control diet containing South American fish oil (FO) was evaluated against feeds with either soybean oil (SO), palm oil (PO) or rapeseed oil (RO). Afterwards, all fish were fed a fish oil finishing diet to determine the progressive recovery of the fillet fatty acid profiles. The results showed that growth and feed utilization in gilthead sea bream are not affected by fish oil substitution with soybean and rapeseed oil, contrary to palm oil inclusion. Flesh and liver docosahexaenoic acid (DHA) and arachidonic acid (ArA) contents were reduced to a lower degree than their reduction in the diet, whereas eicosapentaenoic (EPA) reduction was more pronounced in both tissues. Sensory analysis revealed no difference in the organoleptic characteristics of the dietary groups. However, low acceptance scores were calculated for all treated groups. No histological alterations were seen in gut tissue but liver of the PO group showed intense lipid accumulation. Re-feeding with a fish oil finishing diet for 120 days was not adequate for restoration of DHA, ArA and EPA. Linoleic (LA) and oleic acid (OA) were retained even after 120 days re-feeding with the fish oil diet. © 2009 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The effects of long term feeding (6 months) of commercial diets with low fish meal content and high levels of vegetable oils (69% fish oil substitution level) were determined in gilthead sea bream (110 g). A control diet containing South American fish oil (FO) was evaluated against feeds with either soybean oil (SO), palm oil (PO) or rapeseed oil (RO). Afterwards, all fish were fed a fish oil finishing diet to determine the progressive recovery of the fillet fatty acid profiles. The results showed that growth and feed utilization in gilthead sea bream are not affected by fish oil substitution with soybean and rapeseed oil, contrary to palm oil inclusion. Flesh and liver docosahexaenoic acid (DHA) and arachidonic acid (ArA) contents were reduced to a lower degree than their reduction in the diet, whereas eicosapentaenoic (EPA) reduction was more pronounced in both tissues. Sensory analysis revealed no difference in the organoleptic characteristics of the dietary groups. However, low acceptance scores were calculated for all treated groups. No histological alterations were seen in gut tissue but liver of the PO group showed intense lipid accumulation. Re-feeding with a fish oil finishing diet for 120 days was not adequate for restoration of DHA, ArA and EPA. Linoleic (LA) and oleic acid (OA) were retained even after 120 days re-feeding with the fish oil diet. © 2009 Elsevier B.V. All rights reserved. |
Adamidou, S; Nengas, I; Henry, M; Grigorakis, K; Rigos, G; Nikolopoulou, D; Kotzamanis, Y; Bell, G J; Jauncey, K Aquaculture, 293 (3-4), pp. 263–271, 2009, ISSN: 00448486. @article{adamidou_growth_2009, title = {Growth, feed utilization, health and organoleptic characteristics of European seabass (Dicentrarchus labrax) fed extruded diets including low and high levels of three different legumes}, author = {S Adamidou and I Nengas and M Henry and K Grigorakis and G Rigos and D Nikolopoulou and Y Kotzamanis and G J Bell and K Jauncey}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-67649088041&doi=10.1016%2fj.aquaculture.2009.04.045&partnerID=40&md5=dfaf34a8796e08dbe50d7099ac4f3f40}, doi = {10.1016/j.aquaculture.2009.04.045}, issn = {00448486}, year = {2009}, date = {2009-01-01}, journal = {Aquaculture}, volume = {293}, number = {3-4}, pages = {263--271}, abstract = {Three legumes [field peas (P), chickpeas (CP) and faba beans (B)] were evaluated at two inclusion levels; 170 (L) and 350 g kg- 1 (H) in a 14-week experiment with triplicate groups of 97.9 ± 6.1 g European seabass. A control diet included wheat meal, fish meal (FM) and a mixture of plant ingredients as protein sources. Diets, processed in a twin-screw extruder, were isonitrogenous and isoenergetic. All experimental diets improved growth compared to the control. Weight gain, feed intake, FCR and SGR were improved for fish fed diet CPL, while fish fed diet CPH gave higher FCR. Protein and starch digestibility were highest for the control diet, while fish fed diets CPL and PL showed significantly lower protein ADCs and lower starch ADC (P ≤ 0.05) for diet PH. Among H diets, significantly better fillet yield was found for fish fed diet PH, but no other differences were found in seabass fillet organoleptic characteristics. Serum glucose and cholesterol were elevated in fish fed CP diets, while protein and triacylglycerols did not show significant differences among treatments. Fish fed the test diets showed no significant evidence of either immunosuppression or immunostimulation. Histology of liver, spleen, kidney and foregut revealed no pathological abnormalities. Field peas, chickpeas and faba beans can be included in European seabass diets up to 350 g kg- 1 substituting for wheat with no negative effects on growth performance, carcass composition or organoleptic characteristics. © 2009 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Three legumes [field peas (P), chickpeas (CP) and faba beans (B)] were evaluated at two inclusion levels; 170 (L) and 350 g kg- 1 (H) in a 14-week experiment with triplicate groups of 97.9 ± 6.1 g European seabass. A control diet included wheat meal, fish meal (FM) and a mixture of plant ingredients as protein sources. Diets, processed in a twin-screw extruder, were isonitrogenous and isoenergetic. All experimental diets improved growth compared to the control. Weight gain, feed intake, FCR and SGR were improved for fish fed diet CPL, while fish fed diet CPH gave higher FCR. Protein and starch digestibility were highest for the control diet, while fish fed diets CPL and PL showed significantly lower protein ADCs and lower starch ADC (P ≤ 0.05) for diet PH. Among H diets, significantly better fillet yield was found for fish fed diet PH, but no other differences were found in seabass fillet organoleptic characteristics. Serum glucose and cholesterol were elevated in fish fed CP diets, while protein and triacylglycerols did not show significant differences among treatments. Fish fed the test diets showed no significant evidence of either immunosuppression or immunostimulation. Histology of liver, spleen, kidney and foregut revealed no pathological abnormalities. Field peas, chickpeas and faba beans can be included in European seabass diets up to 350 g kg- 1 substituting for wheat with no negative effects on growth performance, carcass composition or organoleptic characteristics. © 2009 Elsevier B.V. All rights reserved. |
Vatsos, I; Kotzamanis, Y; Vectesi, D; Henry, Morgane; Angelidis, P Short-term of European sea bass (Dicentrarchus labrax) to nitrate. Effects on skin morphology and skin mucous cells. Journal Article Sc. Annals of DDI, 15 , pp. 105–108, 2009. @article{vatsos_short-term_2009, title = {Short-term of European sea bass (Dicentrarchus labrax) to nitrate. Effects on skin morphology and skin mucous cells.}, author = {I Vatsos and Y Kotzamanis and D Vectesi and Morgane Henry and P Angelidis}, year = {2009}, date = {2009-01-01}, journal = {Sc. Annals of DDI}, volume = {15}, pages = {105--108}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2008 |
Fernández, Ignacio; Hontoria, Francisco; Ortiz-Delgado, Juan B; Kotzamanis, Yannis; Estévez, Alicia; Zambonino-Infante, Jose Luis; Gisbert, Enric Aquaculture, 283 (1-4), pp. 102–115, 2008, ISSN: 00448486. @article{fernandez_larval_2008, title = {Larval performance and skeletal deformities in farmed gilthead sea bream (Sparus aurata) fed with graded levels of Vitamin A enriched rotifers (Brachionus plicatilis)}, author = {Ignacio Fernández and Francisco Hontoria and Juan B Ortiz-Delgado and Yannis Kotzamanis and Alicia Estévez and Jose Luis Zambonino-Infante and Enric Gisbert}, url = {https://linkinghub.elsevier.com/retrieve/pii/S004484860800464X}, doi = {10.1016/j.aquaculture.2008.06.037}, issn = {00448486}, year = {2008}, date = {2008-10-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {283}, number = {1-4}, pages = {102--115}, abstract = {Several nutritional studies have found a direct effect of several vitamins in chondrogenic and osteogenic development during early life stages of marine fish species. In the present study, the effect of vitamin A (VA) in gilthead sea bream skeletogenesis was evaluated by means of four different dietary regimes (enriched rotifers) containing increasing levels of total VA (75, 109, 188 and 723 ng total VA mg- 1 DW). Dietary treatments were offered to larvae during the rotifer-feeding phase (4-20 days after hatching), while later all groups were fed with Artemia nauplii and weaned onto the same inert diet. Different dietary doses of VA affected gilthead sea bream larval growth, survival, performance (maturation of the digestive system) and quality (incidence of skeletal deformities). Higher levels of dietary VA than those included in the commercial emulsion for rotifer enrichment led to different levels and typologies of skeletal deformities, indicating that gilthead sea bream larvae were very sensitive to small increases in dietary VA. The degree of ossification was affected by the level of VA in enriched rotifers: the higher amount of VA in the diet, the higher number of skeletal pieces ossified (R = 0.585}, keywords = {}, pubstate = {published}, tppubtype = {article} } Several nutritional studies have found a direct effect of several vitamins in chondrogenic and osteogenic development during early life stages of marine fish species. In the present study, the effect of vitamin A (VA) in gilthead sea bream skeletogenesis was evaluated by means of four different dietary regimes (enriched rotifers) containing increasing levels of total VA (75, 109, 188 and 723 ng total VA mg- 1 DW). Dietary treatments were offered to larvae during the rotifer-feeding phase (4-20 days after hatching), while later all groups were fed with Artemia nauplii and weaned onto the same inert diet. Different dietary doses of VA affected gilthead sea bream larval growth, survival, performance (maturation of the digestive system) and quality (incidence of skeletal deformities). Higher levels of dietary VA than those included in the commercial emulsion for rotifer enrichment led to different levels and typologies of skeletal deformities, indicating that gilthead sea bream larvae were very sensitive to small increases in dietary VA. The degree of ossification was affected by the level of VA in enriched rotifers: the higher amount of VA in the diet, the higher number of skeletal pieces ossified (R = 0.585 |
2007 |
Kotzamanis, Y P; Gisbert, E; Gatesoupe, F J; Infante, Zambonino J; Cahu, C Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology, 147 (1), pp. 205–214, 2007, ISSN: 10956433. @article{kotzamanis_effects_2007, title = {Effects of different dietary levels of fish protein hydrolysates on growth, digestive enzymes, gut microbiota, and resistance to Vibrio anguillarum in European sea bass (Dicentrarchus labrax) larvae}, author = {Y P Kotzamanis and E Gisbert and F J Gatesoupe and J Zambonino Infante and C Cahu}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33947526750&doi=10.1016%2fj.cbpa.2006.12.037&partnerID=40&md5=ea0a2c3ce2b5e68edd11c12ea217eff8}, doi = {10.1016/j.cbpa.2006.12.037}, issn = {10956433}, year = {2007}, date = {2007-01-01}, journal = {Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology}, volume = {147}, number = {1}, pages = {205--214}, abstract = {Two fish protein hydrolysates (FPH) were incorporated into four diets prepared for start-feeding sea bass larvae, at two different levels (10% and 19% of total ingredients): a commercial FPH, CPSP, in which the molecular mass of the main fraction of soluble peptides (51%) was between 500-2500 Da, and an experimental FPH obtained by acidic silage of sardine offal, SH, with a main portion of soluble peptides (54%) ranging from 200 to 500 Da. The diet with 10% of the commercial FPH gave the best results in terms of growth, survival and intestinal development, as evaluated by the early activity of digestive enzymes in the brush border membrane (alkaline phosphatase and aminopeptidase N). This was related to the low level of Vibrio spp. counted in the larvae of group C10. The high dose of FPH, especially in the experimental preparation rich in short peptides, seemed to favour the dominance of Vibrio sp. TYH3, which behaved opportunistically. The effect of the experimental FPH was ambiguous, since early larvae challenged with Vibrio anguillarum were more resistant to the pathogen, especially at high FPH dose (group S19). This might be due either to direct antagonism between V. anguillarum and Vibrio sp. TYH3, or to the stimulation of the immune response in the larvae. These results indicate that different molecular weight fractions and concentrations of feed-soluble peptides may affect the growth performance and immunological status of sea bass larvae. Consequently, a low dose of commercial FPH seems advisable, both for larval development and for the bacterial environment, although further research is required to determine and characterize peptide fractions that may have a beneficial effect on growth and immune response, and to determine their optimal inclusion levels in diets for sea bass larvae. © 2007 Elsevier Inc. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Two fish protein hydrolysates (FPH) were incorporated into four diets prepared for start-feeding sea bass larvae, at two different levels (10% and 19% of total ingredients): a commercial FPH, CPSP, in which the molecular mass of the main fraction of soluble peptides (51%) was between 500-2500 Da, and an experimental FPH obtained by acidic silage of sardine offal, SH, with a main portion of soluble peptides (54%) ranging from 200 to 500 Da. The diet with 10% of the commercial FPH gave the best results in terms of growth, survival and intestinal development, as evaluated by the early activity of digestive enzymes in the brush border membrane (alkaline phosphatase and aminopeptidase N). This was related to the low level of Vibrio spp. counted in the larvae of group C10. The high dose of FPH, especially in the experimental preparation rich in short peptides, seemed to favour the dominance of Vibrio sp. TYH3, which behaved opportunistically. The effect of the experimental FPH was ambiguous, since early larvae challenged with Vibrio anguillarum were more resistant to the pathogen, especially at high FPH dose (group S19). This might be due either to direct antagonism between V. anguillarum and Vibrio sp. TYH3, or to the stimulation of the immune response in the larvae. These results indicate that different molecular weight fractions and concentrations of feed-soluble peptides may affect the growth performance and immunological status of sea bass larvae. Consequently, a low dose of commercial FPH seems advisable, both for larval development and for the bacterial environment, although further research is required to determine and characterize peptide fractions that may have a beneficial effect on growth and immune response, and to determine their optimal inclusion levels in diets for sea bass larvae. © 2007 Elsevier Inc. All rights reserved. |
Giménez, G; Kotzamanis, Y; Hontoria, F; Estévez, A; Gisbert, E Modelling retinoid content in live prey: A tool for evaluating the nutritional requirements and development studies in fish larvae Journal Article Aquaculture, 267 (1-4), pp. 76–82, 2007, ISSN: 00448486. @article{gimenez_modelling_2007, title = {Modelling retinoid content in live prey: A tool for evaluating the nutritional requirements and development studies in fish larvae}, author = {G Giménez and Y Kotzamanis and F Hontoria and A Estévez and E Gisbert}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-34250312482&doi=10.1016%2fj.aquaculture.2007.01.022&partnerID=40&md5=76ef723267befaeab5239bc93646bd7e}, doi = {10.1016/j.aquaculture.2007.01.022}, issn = {00448486}, year = {2007}, date = {2007-01-01}, journal = {Aquaculture}, volume = {267}, number = {1-4}, pages = {76--82}, abstract = {This study was conducted to evaluate the accumulation of different levels of total vitamin A in live prey (Brachionus plicatilis and Artemia nauplii) enriched with a commercial emulsion (0.15 and 0.6 g l- 1 for rotifers and Artemia nauplii, respectively), which contained increasing levels of all-trans retinyl acetate. Emulsions used for rotifer enrichment contained 124, 138, 151, 165, 178, 192, 226, 259 and 327 μg total vitamin A l- 1, whereas those used for Artemia nauplii contained 494, 548, 602, 629, 710, 764, 899, 1034 and 1334 μg total vitamin A l- 1. Total vitamin A incorporation in rotifers was constant until a threshold comprised between 226 and 327 μg total vitamin A l- 1, above which the incorporation of total vitamin A from the emulsion was maximum (806 ng total vitamin A mg DW- 1 in rotifers enriched with 327 μg total vitamin A l- 1). In Artemia nauplii, total vitamin A increased from 4.0 ng mg DW- 1 up to 52 ng mg DW- 1 in nauplii enriched with an emulsion containing 1334 μg total vitamin A l- 1. Retinoid levels in live prey increased as the content of all-trans retinyl acetate augmented in the emulsion, although they did not accumulate in a dose-dependent manner because retinoid incorporation in live prey was found to be not proportional to the content in the emulsion. Rotifers exhibited a higher retinoid incorporation pattern than Artemia nauplii, which seemed to be related to species-specific differences between both live prey. Both live prey were able to absorb and metabolize the vitamin A compounds administered through the emulsion, according to the results regarding retinol and retinoic acid content although the levels were higher in the rotifers than in the nauplii. The differential pattern of total vitamin A accumulation between rotifers and Artemia nauplii should be considered when designing nutritional studies dealing with this vitamin and first feeding marine larvae reared on live prey due to the difficulty in maintaining constant levels of total vitamin A especially during the transition feeding phase from rotifers to Artemia nauplii. © 2007 Elsevier B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study was conducted to evaluate the accumulation of different levels of total vitamin A in live prey (Brachionus plicatilis and Artemia nauplii) enriched with a commercial emulsion (0.15 and 0.6 g l- 1 for rotifers and Artemia nauplii, respectively), which contained increasing levels of all-trans retinyl acetate. Emulsions used for rotifer enrichment contained 124, 138, 151, 165, 178, 192, 226, 259 and 327 μg total vitamin A l- 1, whereas those used for Artemia nauplii contained 494, 548, 602, 629, 710, 764, 899, 1034 and 1334 μg total vitamin A l- 1. Total vitamin A incorporation in rotifers was constant until a threshold comprised between 226 and 327 μg total vitamin A l- 1, above which the incorporation of total vitamin A from the emulsion was maximum (806 ng total vitamin A mg DW- 1 in rotifers enriched with 327 μg total vitamin A l- 1). In Artemia nauplii, total vitamin A increased from 4.0 ng mg DW- 1 up to 52 ng mg DW- 1 in nauplii enriched with an emulsion containing 1334 μg total vitamin A l- 1. Retinoid levels in live prey increased as the content of all-trans retinyl acetate augmented in the emulsion, although they did not accumulate in a dose-dependent manner because retinoid incorporation in live prey was found to be not proportional to the content in the emulsion. Rotifers exhibited a higher retinoid incorporation pattern than Artemia nauplii, which seemed to be related to species-specific differences between both live prey. Both live prey were able to absorb and metabolize the vitamin A compounds administered through the emulsion, according to the results regarding retinol and retinoic acid content although the levels were higher in the rotifers than in the nauplii. The differential pattern of total vitamin A accumulation between rotifers and Artemia nauplii should be considered when designing nutritional studies dealing with this vitamin and first feeding marine larvae reared on live prey due to the difficulty in maintaining constant levels of total vitamin A especially during the transition feeding phase from rotifers to Artemia nauplii. © 2007 Elsevier B.V. All rights reserved. |
Moraiti-Ioannidou, M; Castritsi-Catharios, J; Miliou, H; Kotzamanis, Y P Fatty acid composition and biometry of five Greek Artemia populations suitable for aquaculture purposes Journal Article Aquaculture Research, 38 (15), pp. 1664–1672, 2007, ISSN: 1355557X. @article{moraiti-ioannidou_fatty_2007, title = {Fatty acid composition and biometry of five Greek Artemia populations suitable for aquaculture purposes}, author = {M Moraiti-Ioannidou and J Castritsi-Catharios and H Miliou and Y P Kotzamanis}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-35948966813&doi=10.1111%2fj.1365-2109.2007.01835.x&partnerID=40&md5=278bd71b0d3bf8726f818dbfa97ca58e}, doi = {10.1111/j.1365-2109.2007.01835.x}, issn = {1355557X}, year = {2007}, date = {2007-01-01}, journal = {Aquaculture Research}, volume = {38}, number = {15}, pages = {1664--1672}, abstract = {Evaluation of a new-to-science Artemia population and comparison with four other existing populations were carried out. Five parthenogenetic Artemia populations from the following Greek saltworks were studied: Alyki (a new population), Kalloni, Milos, Polychnitos and Messolongi. The diameters of non-decapsulated and decapsulated cysts as well as the lengths of nauplii instar stages I, II and III were measured. In addition, the fatty acid composition of decapsulated cysts was estimated. The cyst size of the Alyki population was the smallest, with the largest chorion found in Greece so far. The Messolongi population had the largest cysts, with the smallest chorion of all five populations. The diameter of the non-decapsulated cysts was highly related (Ptextless0.01) to the length of the nauplii instar I, II and III. The diameter of decapsulated cysts was found to be the most stable and reliable biometric characteristic. The study of their fatty acids profiles revealed that the Polychnitos and Messolongi populations are suitable as live food for freshwater species, while the Alyki, Milos and Kalloni populations are suitable for marine species, having high levels (8.9%, 11.2% and 12.5% respectively) of eicosapentaenoic acid and detectable amounts (0.3%, 0.1% and 0.2% respectively) of docosahexaenoic acid. © 2007 The Authors.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Evaluation of a new-to-science Artemia population and comparison with four other existing populations were carried out. Five parthenogenetic Artemia populations from the following Greek saltworks were studied: Alyki (a new population), Kalloni, Milos, Polychnitos and Messolongi. The diameters of non-decapsulated and decapsulated cysts as well as the lengths of nauplii instar stages I, II and III were measured. In addition, the fatty acid composition of decapsulated cysts was estimated. The cyst size of the Alyki population was the smallest, with the largest chorion found in Greece so far. The Messolongi population had the largest cysts, with the smallest chorion of all five populations. The diameter of the non-decapsulated cysts was highly related (Ptextless0.01) to the length of the nauplii instar I, II and III. The diameter of decapsulated cysts was found to be the most stable and reliable biometric characteristic. The study of their fatty acids profiles revealed that the Polychnitos and Messolongi populations are suitable as live food for freshwater species, while the Alyki, Milos and Kalloni populations are suitable for marine species, having high levels (8.9%, 11.2% and 12.5% respectively) of eicosapentaenoic acid and detectable amounts (0.3%, 0.1% and 0.2% respectively) of docosahexaenoic acid. © 2007 The Authors. |
Kotzamanis, Y Recent developments on the formulation of compound diets for the early life stages of European sea bass and sea bream larvae Inproceedings Kotzamanis, Y (Ed.): Aquaculture Twin Event -Cost/Eureka Strategic Workshop & Brokerage, Patras, Greece, Patras, Greece, 2007, (Aquaculture Twin Event -Cost/Eureka Strategic Workshop & Brokerage, Patras, Greece). @inproceedings{kotzamanis_recent_2007, title = {Recent developments on the formulation of compound diets for the early life stages of European sea bass and sea bream larvae}, author = {Y Kotzamanis}, editor = {Y Kotzamanis}, year = {2007}, date = {2007-01-01}, booktitle = {Aquaculture Twin Event -Cost/Eureka Strategic Workshop & Brokerage, Patras, Greece}, address = {Patras, Greece}, note = {Aquaculture Twin Event -Cost/Eureka Strategic Workshop & Brokerage, Patras, Greece}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Xylouri, E; Kotzamanis, Y P; Poulou, Athanasso F; Dong, L; Pappas, I S; Argyrokastritis, A; Fragkiadaki, E Isolation, characterization, and sequencing of nodavirus in sturgeon (Acipenser gueldestaedi L.) reared in freshwater facilities Journal Article Israeli Journal of Aquaculture - Bamidgeh, 59 , pp. 36 – 41, 2007. @article{xylouri_isolation_2007, title = {Isolation, characterization, and sequencing of nodavirus in sturgeon (Acipenser gueldestaedi L.) reared in freshwater facilities}, author = {E Xylouri and Y P Kotzamanis and F Athanasso Poulou and L Dong and I S Pappas and A Argyrokastritis and E Fragkiadaki}, url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-34047204411&partnerID=40&md5=613617628f6d5e8ad3ea10829146183e}, year = {2007}, date = {2007-01-01}, journal = {Israeli Journal of Aquaculture - Bamidgeh}, volume = {59}, pages = {36 -- 41}, abstract = {The study demonstrates the presence of a nodavirus that affected sturgeon in fresh water, causing disease with neurological signs. The virus was isolated and inoculated onto SSN-1 (striped snakehead, Channa striatus) cell cultures where cytopathic effects (CPE) of the virus included vacuolation of the cells and degeneration of the monolayer. A 255 bp amplicon from nucleic acid preparations of brain tissue from infected sturgeon was detected by PCR (RT-PCR and nested-PCR) and compared with corresponding amino acid sequences of other infected species. The sequences from the sturgeon were similar to those of sea bass, red spotted grouper, and European eel, supporting the hypothesis that the virus originated in marine fish and was horizontally transmitted to freshwater sturgeon.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The study demonstrates the presence of a nodavirus that affected sturgeon in fresh water, causing disease with neurological signs. The virus was isolated and inoculated onto SSN-1 (striped snakehead, Channa striatus) cell cultures where cytopathic effects (CPE) of the virus included vacuolation of the cells and degeneration of the monolayer. A 255 bp amplicon from nucleic acid preparations of brain tissue from infected sturgeon was detected by PCR (RT-PCR and nested-PCR) and compared with corresponding amino acid sequences of other infected species. The sequences from the sturgeon were similar to those of sea bass, red spotted grouper, and European eel, supporting the hypothesis that the virus originated in marine fish and was horizontally transmitted to freshwater sturgeon. |
2003 |
Tyrpenou, A E; Kotzamanis, Y P; Alexis, M N Aquaculture, 220 (1-4), pp. 633–642, 2003, ISSN: 00448486. @article{tyrpenou_flumequine_2003, title = {Flumequine depletion from muscle plus skin tissue of gilthead seabream (Sparus aurata L.) fed flumequine medicated feed in seawater at 18 and 24 °C}, author = {A E Tyrpenou and Y P Kotzamanis and M N Alexis}, url = {https://linkinghub.elsevier.com/retrieve/pii/S004484860200251X}, doi = {10.1016/S0044-8486(02)00251-X}, issn = {00448486}, year = {2003}, date = {2003-04-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {220}, number = {1-4}, pages = {633--642}, abstract = {We examined flumequine depletion from muscle plus skin of gilthead seabream held in seawater at 18 and 24°C. Seven groups of 10 fish each were sampled at intervals ranging from 24 to 168 h after in-feed administration of flumequine at 35 mg/kg/day for 5 days. Muscle plus skin tissue samples were analyzed for flumequine by high-performance liquid chromatography and fluorescence detection (HPLC-SFD). Parent flumequine concentrations declined rapidly from muscle plus skin after dosing with elimination half-lives of t1/2=22.14 and 21.43 h at 18 and 24°C, respectively. Withdrawal periods for the maximum residue limit (MRL) of 600 μg/kg flumequine in muscle plus skin at 95% tolerance limit were 106.08 and 75.84 h at 18 and 24°C, respectively, after treatment. © 2003 Elsevier Science B.V. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We examined flumequine depletion from muscle plus skin of gilthead seabream held in seawater at 18 and 24°C. Seven groups of 10 fish each were sampled at intervals ranging from 24 to 168 h after in-feed administration of flumequine at 35 mg/kg/day for 5 days. Muscle plus skin tissue samples were analyzed for flumequine by high-performance liquid chromatography and fluorescence detection (HPLC-SFD). Parent flumequine concentrations declined rapidly from muscle plus skin after dosing with elimination half-lives of t1/2=22.14 and 21.43 h at 18 and 24°C, respectively. Withdrawal periods for the maximum residue limit (MRL) of 600 μg/kg flumequine in muscle plus skin at 95% tolerance limit were 106.08 and 75.84 h at 18 and 24°C, respectively, after treatment. © 2003 Elsevier Science B.V. All rights reserved. |
2001 |
Kotzamanis, Y P; Alexis, M N; Andriopoulou, A; Castritsi-Cathariou, I; Fotis, G Utilization of waste material resulting from trout processing in gilthead bream (Sparus aurata L.) diets Journal Article Aquaculture Research, 32 , pp. 288–295, 2001, ISSN: 1355557X, 13652109. @article{kotzamanis_utilization_2001, title = {Utilization of waste material resulting from trout processing in gilthead bream (Sparus aurata L.) diets}, author = {Y P Kotzamanis and M N Alexis and A Andriopoulou and I Castritsi-Cathariou and G Fotis}, url = {http://doi.wiley.com/10.1046/j.1355-557x.2001.00042.x}, doi = {10.1046/j.1355-557x.2001.00042.x}, issn = {1355557X, 13652109}, year = {2001}, date = {2001-12-01}, urldate = {2020-08-18}, journal = {Aquaculture Research}, volume = {32}, pages = {288--295}, abstract = {Fish processing creates a large amount of waste of high nutrient content which, if not properly processed for use in human or animal nutrition, is likely to be deposited in the environment creating pollution problems. Waste parts from rainbow trout processing for smoking, consisting of heads, bones, tails and intestines, were used as feed ingredients for gilthead bream diets. Heads, bones and tails had similar compositions, their weighed mean indicating about 700 g kg-1 moisture, 150 g kg-1 protein and 110 g kg-1 fat. Intestines contained higher lipid (350 g kg-1) and lower moisture (560 g kg-1) and protein content (80 g kg-1). Seasonal changes in composition indicated significant differences. Three experimental diets were formulated having the same proximate composition on a dry weight basis. The control diet (A) contained fish meal as the main protein source and fish oil as the oil supplement. In diet B part of the protein and most of lipid was provided by trout waste and in diet C most of the lipid was provided by trout intestines. Gilthead bream fingerlings of 4 g initial weight were fed to apparent satiation for 72 days, at a temperature of 20°C, to an average final weight of 19 g. All diets were fed in a dry form. The experiment was performed in duplicate. Growth and feed utilization data were high and similar among groups. The body composition of the resulting fish did not show any difference among dietary treatments. Differences in liver lipid and fatty acid content were found between all dietary treatments. The growth and body composition data from this preliminary experiment indicated that trout waste could be used successfully as a dietary ingredient of sea bream diets. © 2001 Blackwell Science Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Fish processing creates a large amount of waste of high nutrient content which, if not properly processed for use in human or animal nutrition, is likely to be deposited in the environment creating pollution problems. Waste parts from rainbow trout processing for smoking, consisting of heads, bones, tails and intestines, were used as feed ingredients for gilthead bream diets. Heads, bones and tails had similar compositions, their weighed mean indicating about 700 g kg-1 moisture, 150 g kg-1 protein and 110 g kg-1 fat. Intestines contained higher lipid (350 g kg-1) and lower moisture (560 g kg-1) and protein content (80 g kg-1). Seasonal changes in composition indicated significant differences. Three experimental diets were formulated having the same proximate composition on a dry weight basis. The control diet (A) contained fish meal as the main protein source and fish oil as the oil supplement. In diet B part of the protein and most of lipid was provided by trout waste and in diet C most of the lipid was provided by trout intestines. Gilthead bream fingerlings of 4 g initial weight were fed to apparent satiation for 72 days, at a temperature of 20°C, to an average final weight of 19 g. All diets were fed in a dry form. The experiment was performed in duplicate. Growth and feed utilization data were high and similar among groups. The body composition of the resulting fish did not show any difference among dietary treatments. Differences in liver lipid and fatty acid content were found between all dietary treatments. The growth and body composition data from this preliminary experiment indicated that trout waste could be used successfully as a dietary ingredient of sea bream diets. © 2001 Blackwell Science Ltd. |
2025 |
Manga, Michael; Tominaga, Masako; Preine, Jonas; Ronge, Thomas A; Beethe, Sarah; Hübscher, Christian; McIntosh, Iona; Nomikou, Paraskevi; Kutterolf, Steffen; Druitt, Tim; Bernard, Alexis; Berthod, Carole; Chen, Hehe; Clark, Acacia; DeBari, Susan; Fernandez‐Perez, Tatiana I; Gertisser, Ralf; Johnston, Raymond M; Jones, Christopher K; Joshi, Batuk K; Kletetschka, Günther; Koukousioura, Olga; McCanta, Molly; Morris, Antony; Pank, Katharina; Peccia, Ally; Polymenakou, Paraskevi N; Woodhouse, Adam; Yamamoto, Yuzuru Low Heat Flow in the Anhydros Basin, Aegean Sea, Recorded by Deep Subsurface Temperatures Journal Article Geophysical Research Letters, 52 (13), pp. e2025GL115919, 2025, ISSN: 0094-8276, 1944-8007. @article{manga_low_2025, title = {Low Heat Flow in the Anhydros Basin, Aegean Sea, Recorded by Deep Subsurface Temperatures}, author = {Michael Manga and Masako Tominaga and Jonas Preine and Thomas A Ronge and Sarah Beethe and Christian Hübscher and Iona McIntosh and Paraskevi Nomikou and Steffen Kutterolf and Tim Druitt and Alexis Bernard and Carole Berthod and Hehe Chen and Acacia Clark and Susan DeBari and Tatiana I Fernandez‐Perez and Ralf Gertisser and Raymond M Johnston and Christopher K Jones and Batuk K Joshi and Günther Kletetschka and Olga Koukousioura and Molly McCanta and Antony Morris and Katharina Pank and Ally Peccia and Paraskevi N Polymenakou and Adam Woodhouse and Yuzuru Yamamoto}, url = {https://agupubs.onlinelibrary.wiley.com/doi/10.1029/2025GL115919 /wp-content/uploads/2025/07/2025-Manga-GRL-36.pdf}, doi = {10.1029/2025GL115919}, issn = {0094-8276, 1944-8007}, year = {2025}, date = {2025-07-16}, urldate = {2025-07-16}, journal = {Geophysical Research Letters}, volume = {52}, number = {13}, pages = {e2025GL115919}, abstract = {Abstract Subseafloor in situ temperatures in a drilled hole in the Anhydros Basin, Aegean Sea, measured during International Ocean Discovery Program (IODP) Expedition 398, yielded a low heat flow (textless0.023 W/m 2 ) despite active magmatism and rifting in the region. The coldest and highest temperatures were 13.9°C at 52.5 m below seafloor (mbsf) and 15.5°C for the deepest measurement at 360.4 mbsf, respectively. Comparison of a heat transfer model with measurements suggests that sea bottom temperatures during the last glacial period were up to 10°C cooler than Holocene temperatures. The magnitude of Holocene warming co‐varies with the geothermal heat flow: if the former goes up the latter goes up. Low heat flow may arise from lateral removal of heat through deeper formations by gravity driven advection of fluids. Tectonic separation of the northwestern Anhydros Basin from the Christiana‐Santorini‐Kolumbo volcanic field may lead to minimal magmatic influences on heat flow. , Plain Language Summary Temperatures in Earth's crust reveal the processes that create and transport heat. In the rifting Anhydros Basin in the Aegean Sea, north of the active South Aegean Volcanic Arc, International Ocean Discovery Program Expedition 398 measured cold subsurface temperatures in a borehole to depths exceeding 300 m below the seafloor. These temperatures record low heat flow and cold seafloor temperatures from the last glacial period. The low heat flow at shallow depths may be due to deeper fluid circulation that removes heat. Low heat flow further implies that there are no magma bodies within the crust in the northwestern part of the Anhydros Basin. , Key Points Heat flow is low in the Anhydros Basin, Aegean Sea Ocean bottom temperatures were cold during the last glacial Subsurface temperature records surface temperature changes and heat flow}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Subseafloor in situ temperatures in a drilled hole in the Anhydros Basin, Aegean Sea, measured during International Ocean Discovery Program (IODP) Expedition 398, yielded a low heat flow (textless0.023 W/m 2 ) despite active magmatism and rifting in the region. The coldest and highest temperatures were 13.9°C at 52.5 m below seafloor (mbsf) and 15.5°C for the deepest measurement at 360.4 mbsf, respectively. Comparison of a heat transfer model with measurements suggests that sea bottom temperatures during the last glacial period were up to 10°C cooler than Holocene temperatures. The magnitude of Holocene warming co‐varies with the geothermal heat flow: if the former goes up the latter goes up. Low heat flow may arise from lateral removal of heat through deeper formations by gravity driven advection of fluids. Tectonic separation of the northwestern Anhydros Basin from the Christiana‐Santorini‐Kolumbo volcanic field may lead to minimal magmatic influences on heat flow. , Plain Language Summary Temperatures in Earth's crust reveal the processes that create and transport heat. In the rifting Anhydros Basin in the Aegean Sea, north of the active South Aegean Volcanic Arc, International Ocean Discovery Program Expedition 398 measured cold subsurface temperatures in a borehole to depths exceeding 300 m below the seafloor. These temperatures record low heat flow and cold seafloor temperatures from the last glacial period. The low heat flow at shallow depths may be due to deeper fluid circulation that removes heat. Low heat flow further implies that there are no magma bodies within the crust in the northwestern part of the Anhydros Basin. , Key Points Heat flow is low in the Anhydros Basin, Aegean Sea Ocean bottom temperatures were cold during the last glacial Subsurface temperature records surface temperature changes and heat flow |
Dritsas, Panagiotis; Patsialou, Stefania; Kampantais, Dimitrios; Roussos, Efstratios; Kotzamanis, Yannis; Tekerlekopoulou, Athanasia; Vayenas, Dimitris V; Aggelis, George Investigating the Potential of Newly Isolated Microalgae Strains from the Ionian Sea (Greece) Cultured in an Open Raceway Pond Journal Article Applied Sciences, 15 (12), pp. 6680, 2025, ISSN: 2076-3417. @article{dritsas_investigating_2025, title = {Investigating the Potential of Newly Isolated Microalgae Strains from the Ionian Sea (Greece) Cultured in an Open Raceway Pond}, author = {Panagiotis Dritsas and Stefania Patsialou and Dimitrios Kampantais and Efstratios Roussos and Yannis Kotzamanis and Athanasia Tekerlekopoulou and Dimitris V Vayenas and George Aggelis}, url = {https://www.mdpi.com/2076-3417/15/12/6680 /wp-content/uploads/2025/07/2025-Dritsas-AppSci-35.pdf}, doi = {10.3390/app15126680}, issn = {2076-3417}, year = {2025}, date = {2025-07-07}, urldate = {2025-07-10}, journal = {Applied Sciences}, volume = {15}, number = {12}, pages = {6680}, abstract = {Microalgae are a subject of interest not only for fundamental research but for various biotechnological applications as well. In this study, the ability of newly isolated strains, i.e., Picochlorum costavermella VAS2.5, Picochlorum oklahomense SAG4.4, Microchloropsis gaditana VON5.3, and Nephroselmis pyriformis PAT2.7, to grow when cultured in an open raceway pond under laboratory conditions and produce various metabolites of high-added value was evaluated. N. pyriformis PAT2.7 and P. costavermella VAS2.5 were the greatest in biomass production (exceeding 0.4 g/L), while P. costavermella VAS2.5 and M. gaditana VON5.3 were the greatest in lipid production (reaching approximately 18%, wt/wt). The lipid fraction of glycolipids and sphingolipids was predominant (43.6–55.4%, wt/wt), followed by neutral lipids (27.1–50.1%, wt/wt) and phospholipids (6.9–17.4%, wt/wt). Picochlora and M. gaditana VON5.3 lipids were rich in Δ5,8,11,14,17C20:5 and/or Δ9,12,15C18:3, while N. pyriformis PAT2.7 synthesized Δ9C16:1 in large quantities (30–40%, wt/wt). All strains showed remarkable yields in polysaccharide and protein production, demonstrated a well-balanced amino acid profile, and synthesized pigments in amounts comparable to other studies. The biochemical profiles of these strains showcased their suitability for use primarily in the aquaculture sector.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Microalgae are a subject of interest not only for fundamental research but for various biotechnological applications as well. In this study, the ability of newly isolated strains, i.e., Picochlorum costavermella VAS2.5, Picochlorum oklahomense SAG4.4, Microchloropsis gaditana VON5.3, and Nephroselmis pyriformis PAT2.7, to grow when cultured in an open raceway pond under laboratory conditions and produce various metabolites of high-added value was evaluated. N. pyriformis PAT2.7 and P. costavermella VAS2.5 were the greatest in biomass production (exceeding 0.4 g/L), while P. costavermella VAS2.5 and M. gaditana VON5.3 were the greatest in lipid production (reaching approximately 18%, wt/wt). The lipid fraction of glycolipids and sphingolipids was predominant (43.6–55.4%, wt/wt), followed by neutral lipids (27.1–50.1%, wt/wt) and phospholipids (6.9–17.4%, wt/wt). Picochlora and M. gaditana VON5.3 lipids were rich in Δ5,8,11,14,17C20:5 and/or Δ9,12,15C18:3, while N. pyriformis PAT2.7 synthesized Δ9C16:1 in large quantities (30–40%, wt/wt). All strains showed remarkable yields in polysaccharide and protein production, demonstrated a well-balanced amino acid profile, and synthesized pigments in amounts comparable to other studies. The biochemical profiles of these strains showcased their suitability for use primarily in the aquaculture sector. |
Vasilaki, Antigoni; Nengas, Ioannis; Fountoulaki, Eleni; Henry, Morgane; Kogiannou, Dimitra; Nikoloudaki, Chrysanthi; Chronopoulos, Petros; Karapanagiotidis, Ioannis T; Mente, Elena Aquaculture, 609 , pp. 742820, 2025, ISSN: 0044-8486. @article{vasilaki_nutritional_2025, title = {Nutritional enhancement of lupin meal (\textit{Lupinus albus}), through fermentation with textitSaccharomyces cerevisiae, as plant protein ingredient in aquafeeds for the European sea bass (\textit{Dicentrarchus labrax}).}, author = {Antigoni Vasilaki and Ioannis Nengas and Eleni Fountoulaki and Morgane Henry and Dimitra Kogiannou and Chrysanthi Nikoloudaki and Petros Chronopoulos and Ioannis T Karapanagiotidis and Elena Mente}, url = {https://www.sciencedirect.com/science/article/pii/S0044848625007069 /wp-content/uploads/2025/07/2025-Vasilaki-AQUA-34.pdf}, doi = {10.1016/j.aquaculture.2025.742820}, issn = {0044-8486}, year = {2025}, date = {2025-07-04}, urldate = {2025-07-09}, journal = {Aquaculture}, volume = {609}, pages = {742820}, abstract = {Legumes contain antinutritional factors that restrict their use in fish nutrition, however, biotechnological methods such as solid-state fermentation can improve their nutritional profile, positioning them as a sustainable alternative for aquafeed formulation. This research evaluated the feasibility of replacing soybean meal with Saccharomyces cerevisiae-fermented lupin meal (Lupinus albus) in feed formulations for European sea bass (Dicentrarchus labrax). Four diets were produced; one commercial type diet (FRL0) and three diets with partial (FRL10, FRL12.5) or complete (FRL15) replacement of soybean meal by fermented lupin meal. European sea bass juveniles, with an initial average weight: 18.9 g, were fed the experimental diets for 71 days in triplicate groups. Results indicated no adverse effects on key production indices when soybean meal was replaced partially or totally. Fermented lupin meal linearly improved feed utilization. Apparent digestibility coefficient of protein significantly increased in groups fed 12.5 % and 15 % fermented lupin, as did apparent digestibility coefficient of fat, compared to the FRL0 group. Trypsin activity in pyloric caeca was statistically elevated in the fermented lupin groups compare to FRL0 group. Whole-body composition, amino acid content, and deposition showed no significant differences among groups. Fermented lupin did not suppress the immune system, and a slight immunostimulatory effect was observed at a 12.5 % inclusion level. No histopathological alterations were detected. Overall, the results demonstrated the efficacy of solid-state fermentation process in enhancing lupin meal, highlighting its potential as a sustainable replacement for soybean meal in feed formulations for Mediterranean marine aquaculture species such us D. labrax.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Legumes contain antinutritional factors that restrict their use in fish nutrition, however, biotechnological methods such as solid-state fermentation can improve their nutritional profile, positioning them as a sustainable alternative for aquafeed formulation. This research evaluated the feasibility of replacing soybean meal with Saccharomyces cerevisiae-fermented lupin meal (Lupinus albus) in feed formulations for European sea bass (Dicentrarchus labrax). Four diets were produced; one commercial type diet (FRL0) and three diets with partial (FRL10, FRL12.5) or complete (FRL15) replacement of soybean meal by fermented lupin meal. European sea bass juveniles, with an initial average weight: 18.9 g, were fed the experimental diets for 71 days in triplicate groups. Results indicated no adverse effects on key production indices when soybean meal was replaced partially or totally. Fermented lupin meal linearly improved feed utilization. Apparent digestibility coefficient of protein significantly increased in groups fed 12.5 % and 15 % fermented lupin, as did apparent digestibility coefficient of fat, compared to the FRL0 group. Trypsin activity in pyloric caeca was statistically elevated in the fermented lupin groups compare to FRL0 group. Whole-body composition, amino acid content, and deposition showed no significant differences among groups. Fermented lupin did not suppress the immune system, and a slight immunostimulatory effect was observed at a 12.5 % inclusion level. No histopathological alterations were detected. Overall, the results demonstrated the efficacy of solid-state fermentation process in enhancing lupin meal, highlighting its potential as a sustainable replacement for soybean meal in feed formulations for Mediterranean marine aquaculture species such us D. labrax. |
Droubogiannis, Stavros; Triga, Andriana; Pavlidis, Michail; Katharios, Pantelis Genome sequences of textitVibrio harveyi VH2 and textitVibrio harveyi Vhp1-sp Journal Article Microbiology Resource Announcements, pp. e00087–25, 2025, ISSN: 2576-098X. @article{droubogiannis_genome_2025, title = {Genome sequences of textitVibrio harveyi VH2 and textitVibrio harveyi Vhp1-sp}, author = {Stavros Droubogiannis and Andriana Triga and Michail Pavlidis and Pantelis Katharios}, editor = {Frank J Stewart}, url = {https://journals.asm.org/doi/10.1128/mra.00087-25 /wp-content/uploads/2025/07/2025-Droubogiannis-MIcro-RA-33.pdf}, doi = {10.1128/mra.00087-25}, issn = {2576-098X}, year = {2025}, date = {2025-06-30}, urldate = {2025-07-01}, journal = {Microbiology Resource Announcements}, pages = {e00087--25}, abstract = {ABSTRACT Vibrio harveyi is a major bacterial pathogen of marine aquatic animals causing significant economic losses in aquaculture. Here, we present the complete genomes of two pathogenic strains, VH2 (5.84 Mb, 44.99% GC) and Vhp1-sp (6.30 Mb, 44.98% GC), which encode numerous virulence factors, resistance genes, and genomic islands.}, keywords = {}, pubstate = {published}, tppubtype = {article} } ABSTRACT Vibrio harveyi is a major bacterial pathogen of marine aquatic animals causing significant economic losses in aquaculture. Here, we present the complete genomes of two pathogenic strains, VH2 (5.84 Mb, 44.99% GC) and Vhp1-sp (6.30 Mb, 44.98% GC), which encode numerous virulence factors, resistance genes, and genomic islands. |
Lancerotto, Stefano; Fakriadis, Ioannis; Papadaki, Maria; Sigelaki, Irini; Giménez, Ignacio; Genoves, Jose Vicente Roig; Meloni, Andrea; Mylonas, Constantinos C Aquaculture, 609 , pp. 742795, 2025, ISSN: 00448486. @article{lancerotto_administration_2025, title = {Administration of single-chain recombinant follicle-stimulating hormone (sdrFsh) and luteinizing hormone (sdrLh) stimulates spermatogenesis, but not vitellogenesis in pre-pubertal greater amberjack (\textit{Seriola dumerili})}, author = {Stefano Lancerotto and Ioannis Fakriadis and Maria Papadaki and Irini Sigelaki and Ignacio Giménez and Jose Vicente Roig Genoves and Andrea Meloni and Constantinos C Mylonas}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848625006817 /wp-content/uploads/2025/06/2025-Lancerotto-AQUA-pre-print32.pdf}, doi = {10.1016/j.aquaculture.2025.742795}, issn = {00448486}, year = {2025}, date = {2025-06-24}, urldate = {2025-06-24}, journal = {Aquaculture}, volume = {609}, pages = {742795}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Keklikoglou, Kleoniki; Mandalakis, Manolis; Fanouraki, Eleftheria; Anastasiou, Thekla I; Kagiampaki, Eirini; Vasileiadou, Katerina; Pavloudi, Christina; Arvanitidis, Christos; Pavlidis, Michail Disrupted darkness: the impact of anthropogenic light at night on melatonin secretion of Hermodice carunculata (Polychaeta, Annelida) Journal Article Photochemical & Photobiological Sciences, 24 (6), pp. 1031–1040, 2025, ISSN: 1474-905X, 1474-9092. @article{keklikoglou_disrupted_2025, title = {Disrupted darkness: the impact of anthropogenic light at night on melatonin secretion of \textit{Hermodice carunculata (Polychaeta, Annelida)}}, author = {Kleoniki Keklikoglou and Manolis Mandalakis and Eleftheria Fanouraki and Thekla I Anastasiou and Eirini Kagiampaki and Katerina Vasileiadou and Christina Pavloudi and Christos Arvanitidis and Michail Pavlidis}, url = {https://link.springer.com/10.1007/s43630-025-00744-5 /wp-content/uploads/2025/06/2025-Keklikoglou-PP-Sci-31.pdf}, doi = {10.1007/s43630-025-00744-5}, issn = {1474-905X, 1474-9092}, year = {2025}, date = {2025-06-20}, urldate = {2025-06-20}, journal = {Photochemical & Photobiological Sciences}, volume = {24}, number = {6}, pages = {1031--1040}, abstract = {Abstract Anthropogenic light at night (ALAN) can have serious impacts on marine environments. Several studies have demonstrated that ALAN disrupts melatonin production, a hormone critical for regulating circadian rhythm. In this study, the effects of ALAN on melatonin and two of its related indolamines were investigated in the annelid Hermodice carunculata . Specifically, melatonin, serotonin and tryptamine levels were measured every three hours over a 24 h period in the heads of the annelids maintained under constant light and a 12 h light/12 h dark photoperiod, representing control conditions. Melatonin concentration was quantified using an enzyme immunoassay, while serotonin and tryptamine were analyzed by liquid chromatography–tandem mass spectrometry. Melatonin levels in annelid heads remained relatively constant with a pronounced peak at 11:00. A similar pattern was observed under constant light, but the melatonin peak shifted to 14:00. However, serotonin and tryptamine did not exhibit any significant diurnal variations due to constant light exposure. These findings suggest that melatonin secretion in H. carunculata is sensitive to ALAN, whereas its related indolamines are potentially not. The disruption of H. carunculata' s melatonin secretion pattern may affect its night-time behavior and reproduction, highlighting the need for further studies to assess the ecological effects of ALAN on various marine invertebrates. Graphical abstract}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Anthropogenic light at night (ALAN) can have serious impacts on marine environments. Several studies have demonstrated that ALAN disrupts melatonin production, a hormone critical for regulating circadian rhythm. In this study, the effects of ALAN on melatonin and two of its related indolamines were investigated in the annelid Hermodice carunculata . Specifically, melatonin, serotonin and tryptamine levels were measured every three hours over a 24 h period in the heads of the annelids maintained under constant light and a 12 h light/12 h dark photoperiod, representing control conditions. Melatonin concentration was quantified using an enzyme immunoassay, while serotonin and tryptamine were analyzed by liquid chromatography–tandem mass spectrometry. Melatonin levels in annelid heads remained relatively constant with a pronounced peak at 11:00. A similar pattern was observed under constant light, but the melatonin peak shifted to 14:00. However, serotonin and tryptamine did not exhibit any significant diurnal variations due to constant light exposure. These findings suggest that melatonin secretion in H. carunculata is sensitive to ALAN, whereas its related indolamines are potentially not. The disruption of H. carunculata' s melatonin secretion pattern may affect its night-time behavior and reproduction, highlighting the need for further studies to assess the ecological effects of ALAN on various marine invertebrates. Graphical abstract |
Oikonomou, Stavroula; Manousi, Domniki; Dimitroglou, Arkadios; Kazlari, Zoi; Loukovitis, Dimitrios; Papanna, Kantham; Tzokas, Konstantinos; Katribouzas, Nikos; Papaharisis, Leonidas; Tsigenopoulos, Costas S; Chatziplis, Dimitrios Validation of QTL associated with resistance to Lernanthropus kroyeri in European seabass Journal Article Aquaculture, 608 , pp. 742763, 2025, ISSN: 00448486. @article{oikonomou_validation_2025, title = {Validation of QTL associated with resistance to Lernanthropus kroyeri in European seabass}, author = {Stavroula Oikonomou and Domniki Manousi and Arkadios Dimitroglou and Zoi Kazlari and Dimitrios Loukovitis and Kantham Papanna and Konstantinos Tzokas and Nikos Katribouzas and Leonidas Papaharisis and Costas S Tsigenopoulos and Dimitrios Chatziplis}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848625006490 /wp-content/uploads/2025/06/2025-Oikonomou-AQUA-30.pdf}, doi = {10.1016/j.aquaculture.2025.742763}, issn = {00448486}, year = {2025}, date = {2025-06-18}, urldate = {2025-06-18}, journal = {Aquaculture}, volume = {608}, pages = {742763}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Rizzo, Lucia; Fernández, Tomás Vega; Necci, Francesca; Grelaud, Michaël; Ziveri, Patrizia; Gerovasileiou, Vasilis; Rossi, Sergio Mediterranean Marine Science, 26 (2), pp. 327–340, 2025, ISSN: 1791-6763, 1108-393X. @article{rizzo_can_2025, title = {Can Marine Animal Forests benefit from existing conservation measures? A systematic approach towards the identification of protected sessile benthic species in the Mediterranean Sea}, author = {Lucia Rizzo and Tomás Vega Fernández and Francesca Necci and Michaël Grelaud and Patrizia Ziveri and Vasilis Gerovasileiou and Sergio Rossi}, url = {https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/38550 /wp-content/uploads/2025/06/2025-Rizzo-MMS-29.pdf}, doi = {10.12681/mms.38550}, issn = {1791-6763, 1108-393X}, year = {2025}, date = {2025-06-17}, urldate = {2025-06-17}, journal = {Mediterranean Marine Science}, volume = {26}, number = {2}, pages = {327--340}, abstract = {Some marine benthic invertebrates increase the structural complexity of the seabed, thereby providing suitable habitats to several associated species, resulting in biodiversity hotspots. Marine Animal Forests (MAFs) encompass a set of the most important marine benthic habitats into which diversified sessile suspension feeders like sponges, corals, sea pens, tube worms, bivalves, bryozoans and ascidians occur. Such a mix of sessile species brings characteristic assemblages and supports important ecosystem functions. In the last decades, some species which form MAFs have been the object of international conventions, EU directives, and national policies aiming to address natural and human-induced disturbances. Effective conservation, monitoring, and restoration actions require summarizing the available information to include MAFs in conservation plans. In the present work, the main international policies for the protection of coastal and marine fauna were screened in order to provide a list of protected species which form Mediterranean MAFs. These international normative documents include the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES), the Convention on the Conservation of European Wildlife and Habitats (Bern Convention), the European Habitats Directive, and the Protocol concerning Specially Protected Areas and Biological Diversity in the Mediterranean (SPA/BD Protocol) of the Barcelona Convention. The analysis of the normative items revealed that 62 Mediterranean potential MAF forming species have been included in one or more legal annexes as protected species. These species belong to different phyla, including Porifera, Cnidaria, Bryozoa and Mollusca. A wider view was proposed by the International Union for Conservation of Nature (IUCN), setting the bases of a legal acknowledgement of the MAFs as one of the largest biomes on Earth. This study represents a first step to obtain a baseline of MAF legal protection framework in order to support the further implementation of management measures aimed at increasing the effective protection of MAFs. Reporting the status of the Mediterranean MAF species that should be considered in management plans and conservation measures will be crucial for policymakers, as well as for mitigating current and future impacts on these distinctive marine environments.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Some marine benthic invertebrates increase the structural complexity of the seabed, thereby providing suitable habitats to several associated species, resulting in biodiversity hotspots. Marine Animal Forests (MAFs) encompass a set of the most important marine benthic habitats into which diversified sessile suspension feeders like sponges, corals, sea pens, tube worms, bivalves, bryozoans and ascidians occur. Such a mix of sessile species brings characteristic assemblages and supports important ecosystem functions. In the last decades, some species which form MAFs have been the object of international conventions, EU directives, and national policies aiming to address natural and human-induced disturbances. Effective conservation, monitoring, and restoration actions require summarizing the available information to include MAFs in conservation plans. In the present work, the main international policies for the protection of coastal and marine fauna were screened in order to provide a list of protected species which form Mediterranean MAFs. These international normative documents include the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES), the Convention on the Conservation of European Wildlife and Habitats (Bern Convention), the European Habitats Directive, and the Protocol concerning Specially Protected Areas and Biological Diversity in the Mediterranean (SPA/BD Protocol) of the Barcelona Convention. The analysis of the normative items revealed that 62 Mediterranean potential MAF forming species have been included in one or more legal annexes as protected species. These species belong to different phyla, including Porifera, Cnidaria, Bryozoa and Mollusca. A wider view was proposed by the International Union for Conservation of Nature (IUCN), setting the bases of a legal acknowledgement of the MAFs as one of the largest biomes on Earth. This study represents a first step to obtain a baseline of MAF legal protection framework in order to support the further implementation of management measures aimed at increasing the effective protection of MAFs. Reporting the status of the Mediterranean MAF species that should be considered in management plans and conservation measures will be crucial for policymakers, as well as for mitigating current and future impacts on these distinctive marine environments. |
Kouvara, Konstantina; Lazou-Laskaridis, Evi; Xirotagarou, Peggy; Christodoulou, Dimitris; Dimas, Xenophon; Geraga, Maria; Giovos, Ioannis; Charitou, Anastasia; Gerovasileiou, Vasilis; Galgani, Francois; Papatheodorou, George Marine Pollution Bulletin, 217 , pp. 118109, 2025, ISSN: 0025326X. @article{kouvara_assessing_2025, title = {Assessing marine litter and its ecological impact on the seafloor of Thermaikos Gulf (NE Mediterranean Sea, Greece): Insights from ROV and diver surveys}, author = {Konstantina Kouvara and Evi Lazou-Laskaridis and Peggy Xirotagarou and Dimitris Christodoulou and Xenophon Dimas and Maria Geraga and Ioannis Giovos and Anastasia Charitou and Vasilis Gerovasileiou and Francois Galgani and George Papatheodorou}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0025326X25005843 /wp-content/uploads/2025/06/2025-Kouvara-MPB-nonOPEN-28.pdf}, doi = {10.1016/j.marpolbul.2025.118109}, issn = {0025326X}, year = {2025}, date = {2025-06-16}, urldate = {2025-06-16}, journal = {Marine Pollution Bulletin}, volume = {217}, pages = {118109}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Asimaki, Adamantia; Psofakis, Pier; Gkalogianni, Elli-Zafeiria; Katouni, Aikaterini-Maria; Berillis, Panagiotis; Kormas, Konstantinos A; Rumbos, Christos I; Athanassiou, Christos G; Vasilaki, Antigoni; Fountoulaki, Eleni; Henry, Morgane; Mente, Eleni; Gisbert, Enric; Karapanagiotidis, Ioannis T Aquaculture Nutrition, 2025 (1), pp. 8885509, 2025, ISSN: 1353-5773, 1365-2095. @article{asimaki_effects_2025, title = {The Effects of Dietary Fishmeal Substitution by Full‐Fat and Defatted textitZophobas morio Larvae Meals on Juvenile Gilthead Seabream (\textit{Sparus aurata}): An Integrative Approach}, author = {Adamantia Asimaki and Pier Psofakis and Elli-Zafeiria Gkalogianni and Aikaterini-Maria Katouni and Panagiotis Berillis and Konstantinos A Kormas and Christos I Rumbos and Christos G Athanassiou and Antigoni Vasilaki and Eleni Fountoulaki and Morgane Henry and Eleni Mente and Enric Gisbert and Ioannis T Karapanagiotidis}, editor = {Lee Seong Wei}, url = {https://onlinelibrary.wiley.com/doi/10.1155/anu/8885509 /wp-content/uploads/2025/06/2025-Asimaki-Aq-Nutr-27.pdf}, doi = {10.1155/anu/8885509}, issn = {1353-5773, 1365-2095}, year = {2025}, date = {2025-06-10}, urldate = {2025-06-10}, journal = {Aquaculture Nutrition}, volume = {2025}, number = {1}, pages = {8885509}, abstract = {This study evaluated the dietary fishmeal substitution by full‐fat (FF) and defatted (DF) Zophobas morio meals regarding growth, feed efficiency, proximate and fatty acid compositions, digestive enzymes activities, histology and midgut microbiota in gilthead seabream ( Sparus aurata ). Juveniles initially weighing 3.4 g were distributed to triplicate groups and fed at satiation six isonitrogenous (8.41%) and isocaloric (21 Mj/kg) diets for 100 days. An insect meal‐free diet was the control (CTRL), two diets contained a FF Z. morio meal at 49 g/kg (FF‐49) and 97 g/kg (FF‐97), and three diets contained a DF Z. morio meal at 58 g/kg (DF‐58), 116 g/kg (DF‐116) and 174 g/kg (DF‐174) at the expense of fishmeal. Neither the form nor the inclusion level of Z. morio meals affected the feed intake of fish denoting a similar acceptability to that of fishmeal. Fish survival, growth and feed efficiency were not impaired by all dietary inclusion levels of Z. morio meals. Proximate composition of fish was altered but without indicating a clear correlation with the form or inclusion level of Z. morio . Increasing inclusions of both forms of Z. morio meals tended to decrease 22:6n‐3, 20:5n‐3, 18:3n‐3 and 18:2n‐6 levels in fish tissues. All fish exhibited similar proteolytic enzyme activities, but the increasing inclusions of both insect meal forms led to gradual increases in the lipase and α‐amylase activities indicating a compensatory mechanism for lipid and carbohydrate digestion. The use of Z. morio meals led to some mild histomorphological changes in the intestine and liver that were more pronounced in fish fed the FF form at the highest inclusion level. Midgut bacterial communities of the groups were similar and dominated by potentially beneficial members of Saccharimonadales and Rhodobacteraceae , except FF‐97 fish that had high abundances of Legionella ‐ and Pandoraea ‐like bacteria. To conclude, Z. morio meal, either FF or DF, is a suitable insect protein for fishmeal substitution towards more sustainable aquafeeds for S. aurata .}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study evaluated the dietary fishmeal substitution by full‐fat (FF) and defatted (DF) Zophobas morio meals regarding growth, feed efficiency, proximate and fatty acid compositions, digestive enzymes activities, histology and midgut microbiota in gilthead seabream ( Sparus aurata ). Juveniles initially weighing 3.4 g were distributed to triplicate groups and fed at satiation six isonitrogenous (8.41%) and isocaloric (21 Mj/kg) diets for 100 days. An insect meal‐free diet was the control (CTRL), two diets contained a FF Z. morio meal at 49 g/kg (FF‐49) and 97 g/kg (FF‐97), and three diets contained a DF Z. morio meal at 58 g/kg (DF‐58), 116 g/kg (DF‐116) and 174 g/kg (DF‐174) at the expense of fishmeal. Neither the form nor the inclusion level of Z. morio meals affected the feed intake of fish denoting a similar acceptability to that of fishmeal. Fish survival, growth and feed efficiency were not impaired by all dietary inclusion levels of Z. morio meals. Proximate composition of fish was altered but without indicating a clear correlation with the form or inclusion level of Z. morio . Increasing inclusions of both forms of Z. morio meals tended to decrease 22:6n‐3, 20:5n‐3, 18:3n‐3 and 18:2n‐6 levels in fish tissues. All fish exhibited similar proteolytic enzyme activities, but the increasing inclusions of both insect meal forms led to gradual increases in the lipase and α‐amylase activities indicating a compensatory mechanism for lipid and carbohydrate digestion. The use of Z. morio meals led to some mild histomorphological changes in the intestine and liver that were more pronounced in fish fed the FF form at the highest inclusion level. Midgut bacterial communities of the groups were similar and dominated by potentially beneficial members of Saccharimonadales and Rhodobacteraceae , except FF‐97 fish that had high abundances of Legionella ‐ and Pandoraea ‐like bacteria. To conclude, Z. morio meal, either FF or DF, is a suitable insect protein for fishmeal substitution towards more sustainable aquafeeds for S. aurata . |
Okeleye, Oluwabusayo Israel; Majstorovic, Jovana; Sudharaka, Anupa; Triga, Adriana; Mandalakis, Manolis; Katharios, Pantelis; Mladineo, Ivona Common carp (Cyprinus carpio) blood cells are modulated by a parasitic antimicrobial peptide, A-2S and Edwardsiella spp. strains Journal Article Fish & Shellfish Immunology, 164 , pp. 110427, 2025, ISSN: 10504648. @article{okeleye_common_2025, title = {Common carp (\textit{Cyprinus carpio}) blood cells are modulated by a parasitic antimicrobial peptide, A-2S and Edwardsiella spp. strains}, author = {Oluwabusayo Israel Okeleye and Jovana Majstorovic and Anupa Sudharaka and Adriana Triga and Manolis Mandalakis and Pantelis Katharios and Ivona Mladineo}, url = {https://linkinghub.elsevier.com/retrieve/pii/S105046482500316X /wp-content/uploads/2025/06/2025-Busayo-FSI-26.pdf}, doi = {10.1016/j.fsi.2025.110427}, issn = {10504648}, year = {2025}, date = {2025-06-06}, urldate = {2025-06-06}, journal = {Fish & Shellfish Immunology}, volume = {164}, pages = {110427}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Cascarano, Maria Chiara; Sevasti, Papadogiorgaki; Katharios, Pantelis Ultrastructural Insights Into a textitCandidatus Parilichlamydia sp. Infection of Gill Goblet Cells in Greater Amberjack Journal Article Journal of Fish Diseases, pp. e14146, 2025, ISSN: 0140-7775, 1365-2761. @article{cascarano_ultrastructural_2025, title = {Ultrastructural Insights Into a textitCandidatus Parilichlamydia sp. Infection of Gill Goblet Cells in Greater Amberjack}, author = {Maria Chiara Cascarano and Papadogiorgaki Sevasti and Pantelis Katharios}, url = {https://onlinelibrary.wiley.com/doi/10.1111/jfd.14146 /wp-content/uploads/2025/05/2025-Cascarano-JFD-25.pdf}, doi = {10.1111/jfd.14146}, issn = {0140-7775, 1365-2761}, year = {2025}, date = {2025-05-28}, urldate = {2025-05-28}, journal = {Journal of Fish Diseases}, pages = {e14146}, abstract = {ABSTRACT Despite recent genomic studies and increased molecular data, epitheliocystis remains an enigmatic fish disease with no experimental in vitro or in vivo models to aid the advancement of research. In this study, we revert to a classical microscopical approach and screen with the electron microscope the epitheliocystis lesions caused by a Ca . Parilichlamydia sp., infecting mucus cells in Greater amberjack. We report distinct morphological features of this bacterial family, characterised by Intermediate Bodies that closely resemble those of previously described Candidatus similchlamydia , and Elementary Bodies that exhibit morphological similarities to Chlamydia trachomatis . We describe the characteristics of a novel Chlamydial Inclusion Membrane (IM) type, with abundant interdigitations, possibly shaped by fusion of the IM with cytoplasmic vesicles, and moreover discuss the presence of multivesicular bodies in the infected cell. Our observation of immune cells in the infected areas indicates an interaction of macrophages with infected cells, a role for granular cells as pathogens reservoirs and an active phagoptosis process in the nearby areas, overall shedding light on cellular immune processes characterising these infections in fish hosts.}, keywords = {}, pubstate = {published}, tppubtype = {article} } ABSTRACT Despite recent genomic studies and increased molecular data, epitheliocystis remains an enigmatic fish disease with no experimental in vitro or in vivo models to aid the advancement of research. In this study, we revert to a classical microscopical approach and screen with the electron microscope the epitheliocystis lesions caused by a Ca . Parilichlamydia sp., infecting mucus cells in Greater amberjack. We report distinct morphological features of this bacterial family, characterised by Intermediate Bodies that closely resemble those of previously described Candidatus similchlamydia , and Elementary Bodies that exhibit morphological similarities to Chlamydia trachomatis . We describe the characteristics of a novel Chlamydial Inclusion Membrane (IM) type, with abundant interdigitations, possibly shaped by fusion of the IM with cytoplasmic vesicles, and moreover discuss the presence of multivesicular bodies in the infected cell. Our observation of immune cells in the infected areas indicates an interaction of macrophages with infected cells, a role for granular cells as pathogens reservoirs and an active phagoptosis process in the nearby areas, overall shedding light on cellular immune processes characterising these infections in fish hosts. |
Siaperas, Romanos; Taxeidis, George; Gioti, Anastasia; Nikolaivits, Efstratios; Topakas, Evangelos Multi-omics insights into the response of Aspergillus parasiticus to long-chain alkanes in relation to polyethylene modification Journal Article Environmental Pollution, 376 , pp. 126386, 2025, ISSN: 02697491. @article{siaperas_multi-omics_2025, title = {Multi-omics insights into the response of Aspergillus parasiticus to long-chain alkanes in relation to polyethylene modification}, author = {Romanos Siaperas and George Taxeidis and Anastasia Gioti and Efstratios Nikolaivits and Evangelos Topakas}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0269749125007596 /wp-content/uploads/2025/05/2025-Siaperas-EnvPol-24.pdf}, doi = {10.1016/j.envpol.2025.126386}, issn = {02697491}, year = {2025}, date = {2025-05-22}, urldate = {2025-05-22}, journal = {Environmental Pollution}, volume = {376}, pages = {126386}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Díaz-Avello, Ulises G; Skouridou, Vasso; Shkembi, Xhensila; Reverté, Jaume; Mandalakis, Manolis; Peristeraki, Panagiota; Campàs, Mònica; O'Sullivan, Ciara K Aptamer-antibody sandwich lateral flow test for rapid visual detection of tetrodotoxin in pufferfish Journal Article Science of The Total Environment, 978 , pp. 179419, 2025, ISSN: 00489697. @article{diaz-avello_aptamer-antibody_2025, title = {Aptamer-antibody sandwich lateral flow test for rapid visual detection of tetrodotoxin in pufferfish}, author = {Ulises G Díaz-Avello and Vasso Skouridou and Xhensila Shkembi and Jaume Reverté and Manolis Mandalakis and Panagiota Peristeraki and Mònica Campàs and Ciara K O'Sullivan}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0048969725010563 /wp-content/uploads/2025/05/2025-Díaz-Avello-TTX_LFA-test-DRAFT-SUBMITTED-23.pdf}, doi = {10.1016/j.scitotenv.2025.179419}, issn = {00489697}, year = {2025}, date = {2025-05-20}, urldate = {2025-05-20}, journal = {Science of The Total Environment}, volume = {978}, pages = {179419}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ventriglia, Gianluca; Duncan, Neil; Giménez, Ignacio; Mylonas, Constantinos C; Pousis, Chrysovalentinos; Corriero, Aldo; Zupa, Rosa Spermatogenesis advancement in pre-pubertal meagre Argyrosomus regius treated with recombinant gonadotropins Journal Article Scientific Reports, 15 (1), pp. 15113, 2025, ISSN: 2045-2322. @article{ventriglia_spermatogenesis_2025, title = {Spermatogenesis advancement in pre-pubertal meagre Argyrosomus regius treated with recombinant gonadotropins}, author = {Gianluca Ventriglia and Neil Duncan and Ignacio Giménez and Constantinos C Mylonas and Chrysovalentinos Pousis and Aldo Corriero and Rosa Zupa}, url = {https://www.nature.com/articles/s41598-025-99372-3 /wp-content/uploads/2025/05/2025-Ventriglia-SciRep-22.pdf}, doi = {10.1038/s41598-025-99372-3}, issn = {2045-2322}, year = {2025}, date = {2025-05-13}, urldate = {2025-05-13}, journal = {Scientific Reports}, volume = {15}, number = {1}, pages = {15113}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Papadaki, Maria; Le, Ngoc-Son; Mylonas, Constantinos C; Sarropoulou, Elena Marine Biotechnology, 27 (2), pp. 74, 2025, ISSN: 1436-2228, 1436-2236. @article{papadaki_exploring_2025, title = {Exploring the Fanconi Anemia Gene Expression and Regulation by MicroRNAs in Gilthead Seabream (Sparus aurata) at Different Gonadal Development Stages}, author = {Maria Papadaki and Ngoc-Son Le and Constantinos C Mylonas and Elena Sarropoulou}, url = {https://link.springer.com/10.1007/s10126-025-10444-x /wp-content/uploads/2025/05/2025-Papadaki-MarBioteck-21.pdf}, doi = {10.1007/s10126-025-10444-x}, issn = {1436-2228, 1436-2236}, year = {2025}, date = {2025-05-06}, urldate = {2025-05-06}, journal = {Marine Biotechnology}, volume = {27}, number = {2}, pages = {74}, abstract = {Abstract Fanconi anaemia (FA) is a rare autosomal recessive disease in humans that is distributed worldwide. Fanconi anemia complementation (Fanc) proteins are essential for the appropriate functioning of the FA DNA repair pathway. They are also linked to a number of other biological processes, including oxygen metabolism, cell cycle regulation, haematopoiesis and apoptosis. So far, little research has been conducted on teleosts, but evidence shows that Fanc proteins play a significant role in immune response and sex reversal. For the examination of the expression of three fanc genes ( fancc , fancl, and fancd2 ), as well as the potential regulation of these genes by microRNAs (miRNAs) in gonadal tissues at different stages of development, the present study has selected the gilthead seabream ( Sparus aurata ), a significant aquaculture species that exhibits protandrous hermaphroditism. The obtained data suggested the role of fancl and fancd2 in the maturation of female gonads and the miRNAs miR-210, miR-217 and miR-10926 have been identified as putative regulators of fancd2 , fancc and fancl , respectively. Overall, the data indicated the potential use of fancl and fancd2 genes as sex biomarkers in conjunction with their respective regulation by miRNAs. To the best of our knowledge, this is the first study demonstrating the importance of fanc genes, along with putative regulatory miRNAs, in the reproduction of an important marine aquaculture species.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Fanconi anaemia (FA) is a rare autosomal recessive disease in humans that is distributed worldwide. Fanconi anemia complementation (Fanc) proteins are essential for the appropriate functioning of the FA DNA repair pathway. They are also linked to a number of other biological processes, including oxygen metabolism, cell cycle regulation, haematopoiesis and apoptosis. So far, little research has been conducted on teleosts, but evidence shows that Fanc proteins play a significant role in immune response and sex reversal. For the examination of the expression of three fanc genes ( fancc , fancl, and fancd2 ), as well as the potential regulation of these genes by microRNAs (miRNAs) in gonadal tissues at different stages of development, the present study has selected the gilthead seabream ( Sparus aurata ), a significant aquaculture species that exhibits protandrous hermaphroditism. The obtained data suggested the role of fancl and fancd2 in the maturation of female gonads and the miRNAs miR-210, miR-217 and miR-10926 have been identified as putative regulators of fancd2 , fancc and fancl , respectively. Overall, the data indicated the potential use of fancl and fancd2 genes as sex biomarkers in conjunction with their respective regulation by miRNAs. To the best of our knowledge, this is the first study demonstrating the importance of fanc genes, along with putative regulatory miRNAs, in the reproduction of an important marine aquaculture species. |
Thieme, Philipp; Fischbach, Vivian; Papadakis, Ioannis; Moritz, Timo Development of convergent adaptations reveal highly conserved early ontogenetic skull shape in fishes with amphibious vision Journal Article Scientific Reports, 15 (1), pp. 8411, 2025, ISSN: 2045-2322. @article{thieme_development_2025, title = {Development of convergent adaptations reveal highly conserved early ontogenetic skull shape in fishes with amphibious vision}, author = {Philipp Thieme and Vivian Fischbach and Ioannis Papadakis and Timo Moritz}, url = {https://www.nature.com/articles/s41598-025-91563-2 /wp-content/uploads/2025/04/2025-Thieme-SR-20.pdf}, doi = {10.1038/s41598-025-91563-2}, issn = {2045-2322}, year = {2025}, date = {2025-04-28}, urldate = {2025-04-28}, journal = {Scientific Reports}, volume = {15}, number = {1}, pages = {8411}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Kapelonis, Zacharias; Chatzigeorgiou, Georgios; Ntoumas, Manolis; Grigoriou, Panos; Pettas, Manos; Michelinakis, Spyros; Correia, Ricardo; Lemos, Catarina Rasquilha; Pinheiro, Luis Menezes; Lomba, Caio; Fortuna, João; Loureiro, Rui; Santos, André; Chatzinikolaou, Eva Flying Robots Teach Floating Robots—A Machine Learning Approach for Marine Habitat Mapping Based on Combined Datasets Journal Article Journal of Marine Science and Engineering, 13 (3), pp. 611, 2025, ISSN: 2077-1312. @article{kapelonis_flying_2025, title = {Flying Robots Teach Floating Robots—A Machine Learning Approach for Marine Habitat Mapping Based on Combined Datasets}, author = {Zacharias Kapelonis and Georgios Chatzigeorgiou and Manolis Ntoumas and Panos Grigoriou and Manos Pettas and Spyros Michelinakis and Ricardo Correia and Catarina Rasquilha Lemos and Luis Menezes Pinheiro and Caio Lomba and João Fortuna and Rui Loureiro and André Santos and Eva Chatzinikolaou}, url = {https://www.mdpi.com/2077-1312/13/3/611 /wp-content/uploads/2025/04/2025-Kapelonis-Mar-Sci-Eng-19.pdf}, doi = {10.3390/jmse13030611}, issn = {2077-1312}, year = {2025}, date = {2025-04-11}, urldate = {2025-04-11}, journal = {Journal of Marine Science and Engineering}, volume = {13}, number = {3}, pages = {611}, abstract = {Unmanned aerial and autonomous surface vehicles (UAVs and ASVs, respectively) are two emerging technologies for the mapping of coastal and marine environments. Using UAV photogrammetry, the sea-bottom composition can be resolved with very high fidelity in shallow waters. At greater depths, acoustic methodologies have far better propagation properties compared to optics; therefore, ASVs equipped with multibeam echosounders (MBES) are better-suited for mapping applications in deeper waters. In this work, a sea-bottom classification methodology is presented for mapping the protected habitat of Mediterranean seagrass Posidonia oceanica (habitat code 1120) in a coastal subregion of Heraklion (Crete, Greece). The methodology implements a machine learning scheme, where knowledge obtained from UAV imagery is embedded (through training) into a classifier that utilizes acoustic backscatter intensity and features derived from the MBES data provided by an ASV. Accuracy and precision scores of greater than 85% compared with visual census ground-truth data for both optical and acoustic classifiers indicate that this hybrid mapping approach is promising to mitigate the depth-induced bias in UAV-only models. The latter is especially interesting in cases where the studied habitat boundaries extend beyond depths that can be studied via aerial devices’ optics, as is the case with P. oceanica meadows.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Unmanned aerial and autonomous surface vehicles (UAVs and ASVs, respectively) are two emerging technologies for the mapping of coastal and marine environments. Using UAV photogrammetry, the sea-bottom composition can be resolved with very high fidelity in shallow waters. At greater depths, acoustic methodologies have far better propagation properties compared to optics; therefore, ASVs equipped with multibeam echosounders (MBES) are better-suited for mapping applications in deeper waters. In this work, a sea-bottom classification methodology is presented for mapping the protected habitat of Mediterranean seagrass Posidonia oceanica (habitat code 1120) in a coastal subregion of Heraklion (Crete, Greece). The methodology implements a machine learning scheme, where knowledge obtained from UAV imagery is embedded (through training) into a classifier that utilizes acoustic backscatter intensity and features derived from the MBES data provided by an ASV. Accuracy and precision scores of greater than 85% compared with visual census ground-truth data for both optical and acoustic classifiers indicate that this hybrid mapping approach is promising to mitigate the depth-induced bias in UAV-only models. The latter is especially interesting in cases where the studied habitat boundaries extend beyond depths that can be studied via aerial devices’ optics, as is the case with P. oceanica meadows. |
Mukiibi, Robert; Ferraresso, Serena; Franch, Rafaella; Peruzza, Luca; Rovere, Giulia Dalla; Babbucci, Massimiliano; Bertotto, Daniela; Toffan, Anna; Pascoli, Francesco; Faggion, Sara; Peñaloza, Carolina; Tsigenopoulos, Costas S; Houston, Ross D; Bargelloni, Luca; Robledo, Diego Integrated functional genomic analysis identifies regulatory variants underlying a major QTL for disease resistance in European sea bass Journal Article BMC Biology, 23 (1), pp. 75, 2025, ISSN: 1741-7007. @article{mukiibi_integrated_2025, title = {Integrated functional genomic analysis identifies regulatory variants underlying a major QTL for disease resistance in European sea bass}, author = {Robert Mukiibi and Serena Ferraresso and Rafaella Franch and Luca Peruzza and Giulia Dalla Rovere and Massimiliano Babbucci and Daniela Bertotto and Anna Toffan and Francesco Pascoli and Sara Faggion and Carolina Peñaloza and Costas S Tsigenopoulos and Ross D Houston and Luca Bargelloni and Diego Robledo}, url = {https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-025-02180-4 /wp-content/uploads/2025/04/2025-Mukiibi-BMC-Biology-18.pdf}, doi = {10.1186/s12915-025-02180-4}, issn = {1741-7007}, year = {2025}, date = {2025-04-07}, urldate = {2025-04-07}, journal = {BMC Biology}, volume = {23}, number = {1}, pages = {75}, abstract = {Abstract Background Viral nervous necrosis (VNN) is an important viral disease threatening global aquaculture sustainability and affecting over 50 farmed and ecologically important fish species. A major QTL for resistance to VNN has been previously detected in European sea bass, but the underlying causal gene(s) and mutation(s) remain unknown. To identify the mechanisms and genetic factors underpinning resistance to VNN in European sea bass, we employed integrative analyses of multiple functional genomics assays in European sea bass. Results The estimated heritability of VNN resistance was high ( h 2 textasciitilde 0.40), and a major QTL explaining up to 38% of the genetic variance of the trait was confirmed on chromosome 3, with individuals with the resistant QTL genotype showing a 90% survivability against a VNN outbreak. Whole-genome resequencing analyses narrowed the location of this QTL to a small region containing 4 copies of interferon alpha inducible protein 27-like 2A ( IFI27L2A ) genes, and one copy of the interferon alpha inducible protein 27-like 2 ( IFI27L2 ) gene. RNA sequencing revealed a clear association between the QTL genotype and the expression of two of the IFI27L2A genes, and the IFI27L2 gene. Integration with chromatin accessibility and histone modification data pinpointed two SNPs in active regulatory regions of two of these genes ( IFI27L2A and IFI27L2 ), and transcription factor binding site gains for the resistant alleles were predicted. These alleles, particularly the SNP variant CHR3:10,077,301, exhibited higher frequencies (0.55 to 0.77) in Eastern Mediterranean Sea bass populations, which show considerably higher levels of resistance to VNN, as compared to susceptible West Mediterranean and Atlantic populations (0.15–0.25). Conclusions The SNP variant CHR3:10,077,301, through modulation of IFI27L2 and IFI27L2A genes, is likely the causative mutation underlying resistance to VNN in European sea bass. This is one of the first causative mutations discovered for disease resistance traits in fish and paves the way for marker-assisted selection as well as biotechnological approaches to enhance resistance to VNN in European sea bass and other susceptible species.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Background Viral nervous necrosis (VNN) is an important viral disease threatening global aquaculture sustainability and affecting over 50 farmed and ecologically important fish species. A major QTL for resistance to VNN has been previously detected in European sea bass, but the underlying causal gene(s) and mutation(s) remain unknown. To identify the mechanisms and genetic factors underpinning resistance to VNN in European sea bass, we employed integrative analyses of multiple functional genomics assays in European sea bass. Results The estimated heritability of VNN resistance was high ( h 2 textasciitilde 0.40), and a major QTL explaining up to 38% of the genetic variance of the trait was confirmed on chromosome 3, with individuals with the resistant QTL genotype showing a 90% survivability against a VNN outbreak. Whole-genome resequencing analyses narrowed the location of this QTL to a small region containing 4 copies of interferon alpha inducible protein 27-like 2A ( IFI27L2A ) genes, and one copy of the interferon alpha inducible protein 27-like 2 ( IFI27L2 ) gene. RNA sequencing revealed a clear association between the QTL genotype and the expression of two of the IFI27L2A genes, and the IFI27L2 gene. Integration with chromatin accessibility and histone modification data pinpointed two SNPs in active regulatory regions of two of these genes ( IFI27L2A and IFI27L2 ), and transcription factor binding site gains for the resistant alleles were predicted. These alleles, particularly the SNP variant CHR3:10,077,301, exhibited higher frequencies (0.55 to 0.77) in Eastern Mediterranean Sea bass populations, which show considerably higher levels of resistance to VNN, as compared to susceptible West Mediterranean and Atlantic populations (0.15–0.25). Conclusions The SNP variant CHR3:10,077,301, through modulation of IFI27L2 and IFI27L2A genes, is likely the causative mutation underlying resistance to VNN in European sea bass. This is one of the first causative mutations discovered for disease resistance traits in fish and paves the way for marker-assisted selection as well as biotechnological approaches to enhance resistance to VNN in European sea bass and other susceptible species. |
Wei, Xiao-Lei; Hao, Zhi-Wei; Kotzamanis, Yannis P; Zhang, Tian-Hua; Liu, Zhi-Bo; Yang, Hong; Luo, Zhi Aquaculture Reports, 42 , pp. 102739, 2025, ISSN: 23525134. @article{wei_dietary_2025, title = {Dietary iron oxide (Fe2O3) nanoparticles modulate growth performance, body composition, mineral content and intestinal health of yellow catfish juveniles (Pelteobagrus fulvidraco)}, author = {Xiao-Lei Wei and Zhi-Wei Hao and Yannis P Kotzamanis and Tian-Hua Zhang and Zhi-Bo Liu and Hong Yang and Zhi Luo}, url = {https://linkinghub.elsevier.com/retrieve/pii/S2352513425001255 /wp-content/uploads/2025/03/2025-Wei-AquaReports-16.pdf}, doi = {10.1016/j.aqrep.2025.102739}, issn = {23525134}, year = {2025}, date = {2025-03-31}, urldate = {2025-03-31}, journal = {Aquaculture Reports}, volume = {42}, pages = {102739}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Antoniou, Theodora; Dimitriou, Andreas C; Karameta, Emmanouela; Antoniou, Aglaia; Poulakakis, Nikos; Sfenthourakis, Spyros Comparative phylogeography of four lizard taxa within an oceanic island Journal Article Molecular Phylogenetics and Evolution, 205 , pp. 108295, 2025, ISSN: 10557903. @article{antoniou_comparative_2025, title = {Comparative phylogeography of four lizard taxa within an oceanic island}, author = {Theodora Antoniou and Andreas C Dimitriou and Emmanouela Karameta and Aglaia Antoniou and Nikos Poulakakis and Spyros Sfenthourakis}, url = {https://linkinghub.elsevier.com/retrieve/pii/S1055790325000120 /wp-content/uploads/2025/03/2025-Antoniou-CompPhylo-pre-print-15.pdf}, doi = {10.1016/j.ympev.2025.108295}, issn = {10557903}, year = {2025}, date = {2025-03-24}, urldate = {2025-03-24}, journal = {Molecular Phylogenetics and Evolution}, volume = {205}, pages = {108295}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Droubogiannis, Stavros; Katharios, Pantelis Phage Therapy in Aquaculture Incollection Elumalai, Preetham; Lakshmi, Sreeja (Ed.): Antimicrobial Resistance in Aquaculture and Aquatic Environments, pp. 229–255, Springer Nature Singapore, Singapore, 2025, ISBN: 9789819773190 9789819773206. @incollection{elumalai_phage_2025, title = {Phage Therapy in Aquaculture}, author = {Stavros Droubogiannis and Pantelis Katharios}, editor = {Preetham Elumalai and Sreeja Lakshmi}, url = {https://link.springer.com/10.1007/978-981-97-7320-6_10 /wp-content/uploads/2025/03/2025-Droumbogiannis-and-Katharios-phage-therapy-in-aquaculture-pre-print-14.pdf}, doi = {10.1007/978-981-97-7320-6_10}, isbn = {9789819773190 9789819773206}, year = {2025}, date = {2025-03-20}, urldate = {2025-03-20}, booktitle = {Antimicrobial Resistance in Aquaculture and Aquatic Environments}, pages = {229--255}, publisher = {Springer Nature Singapore}, address = {Singapore}, keywords = {}, pubstate = {published}, tppubtype = {incollection} } |
Chalmoukis, Charalampos; Droubogiannis, Stavros; Michalopoulou, Vassiliki A; Triga, Adriana; Sarris, Panagiotis F; Katharios, Pantelis Pathogens, 14 (3), pp. 247, 2025, ISSN: 2076-0817. @article{chalmoukis_development_2025, title = {Development and Characterization of Fluorescent Protein-Tagged Vibrio harveyi Strains as a Versatile Tool for Studying Infection Dynamics and Strain Interactions}, author = {Charalampos Chalmoukis and Stavros Droubogiannis and Vassiliki A Michalopoulou and Adriana Triga and Panagiotis F Sarris and Pantelis Katharios}, url = {https://www.mdpi.com/2076-0817/14/3/247 /wp-content/uploads/2025/03/2025-Chalmoukis-pathogens-13.pdf}, doi = {10.3390/pathogens14030247}, issn = {2076-0817}, year = {2025}, date = {2025-03-18}, urldate = {2025-03-18}, journal = {Pathogens}, volume = {14}, number = {3}, pages = {247}, abstract = {Fluorescent protein-tagged bacterial strains are widely used tools for studying host-pathogen interactions and microbial dynamics. In this study, we developed and characterized Vibrio harveyi strains genetically modified to express green fluorescent protein (GFP) and red fluorescent protein (RFP). These strains were constructed using triparental mating and evaluated for phenotypic, genomic, and virulence attributes. Genomic analyses revealed strain-specific variations, including mutations in key regulatory and metabolic genes, such as luxO and transketolase. While plasmid acquisition imposed metabolic costs, resulting in altered growth and antibiotic sensitivities in certain transconjugants, others demonstrated robust phenotypic stability. Virulence assays using gilthead seabream larvae revealed that most tagged strains retained moderate pathogenicity, with visualization of co-infections highlighting the potential for studying strain-specific interactions. Furthermore, fluorescent microscopy confirmed the successful colonization and localization of tagged bacteria within host tissues. These findings underscore the utility of GFP- and RFP-tagged Vibrio harveyi as versatile tools for infection dynamics, offering a foundation for future research on strain interactions and pathogen-host relationships.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Fluorescent protein-tagged bacterial strains are widely used tools for studying host-pathogen interactions and microbial dynamics. In this study, we developed and characterized Vibrio harveyi strains genetically modified to express green fluorescent protein (GFP) and red fluorescent protein (RFP). These strains were constructed using triparental mating and evaluated for phenotypic, genomic, and virulence attributes. Genomic analyses revealed strain-specific variations, including mutations in key regulatory and metabolic genes, such as luxO and transketolase. While plasmid acquisition imposed metabolic costs, resulting in altered growth and antibiotic sensitivities in certain transconjugants, others demonstrated robust phenotypic stability. Virulence assays using gilthead seabream larvae revealed that most tagged strains retained moderate pathogenicity, with visualization of co-infections highlighting the potential for studying strain-specific interactions. Furthermore, fluorescent microscopy confirmed the successful colonization and localization of tagged bacteria within host tissues. These findings underscore the utility of GFP- and RFP-tagged Vibrio harveyi as versatile tools for infection dynamics, offering a foundation for future research on strain interactions and pathogen-host relationships. |
Costanzo, Di F; Marsico, Di M; Orefice, I; Kristoffersen, J B; Kasapidis, P; Chaumier, T; Ambrosino, L; Miralto, M; Cigliano, Aiese R; Verret, F; Tirichine, L; Trindade, M; Zyl, Van L; Dato, Di V; Romano, G High-quality genome assembly and annotation of Thalassiosira rotula (synonym of Thalassiosira gravida) Journal Article Scientific Data, 12 (1), pp. 310, 2025, ISSN: 2052-4463. @article{di_costanzo_high-quality_2025, title = {High-quality genome assembly and annotation of Thalassiosira rotula (synonym of Thalassiosira gravida)}, author = {F Di Costanzo and M Di Marsico and I Orefice and J B Kristoffersen and P Kasapidis and T Chaumier and L Ambrosino and M Miralto and R Aiese Cigliano and F Verret and L Tirichine and M Trindade and L Van Zyl and V Di Dato and G Romano}, url = {https://www.nature.com/articles/s41597-025-04634-4 /wp-content/uploads/2025/03/2025-Costanzo-SciData-12.pdf}, doi = {10.1038/s41597-025-04634-4}, issn = {2052-4463}, year = {2025}, date = {2025-03-14}, urldate = {2025-03-14}, journal = {Scientific Data}, volume = {12}, number = {1}, pages = {310}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Sarropoulou, Elena; Katharios, Pantelis; Kaitetzidou, Elisavet; Scapigliati, Giuseppe; Miccoli, Andrea Circulating miRNAs involved in the immune response of the European seabass (Dicentrarchus labrax) Journal Article Fish & Shellfish Immunology, 160 , pp. 110232, 2025, ISSN: 10504648. @article{sarropoulou_circulating_2025, title = {Circulating miRNAs involved in the immune response of the European seabass (Dicentrarchus labrax)}, author = {Elena Sarropoulou and Pantelis Katharios and Elisavet Kaitetzidou and Giuseppe Scapigliati and Andrea Miccoli}, url = {https://linkinghub.elsevier.com/retrieve/pii/S1050464825001214 /wp-content/uploads/2025/03/2025-Sarropoulou-FishShelImm-11.pdf}, doi = {10.1016/j.fsi.2025.110232}, issn = {10504648}, year = {2025}, date = {2025-03-10}, urldate = {2025-03-11}, journal = {Fish & Shellfish Immunology}, volume = {160}, pages = {110232}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Katharios, Pantelis; Karageorgou, Evangelia Protozoan Co‐Infection Drives Fish Mortality Event in Crete's Karteros River Journal Article Journal of Fish Diseases, pp. e14099, 2025, ISSN: 0140-7775, 1365-2761. @article{katharios_protozoan_2025, title = {Protozoan Co‐Infection Drives Fish Mortality Event in Crete's Karteros River}, author = {Pantelis Katharios and Evangelia Karageorgou}, url = {https://onlinelibrary.wiley.com/doi/10.1111/jfd.14099 /wp-content/uploads/2025/03/2025-Katharios-JFD-10.pdf}, doi = {10.1111/jfd.14099}, issn = {0140-7775, 1365-2761}, year = {2025}, date = {2025-03-07}, urldate = {2025-03-10}, journal = {Journal of Fish Diseases}, pages = {e14099}, abstract = {ABSTRACT In August 2024, a significant fish kill involving hundreds of flathead grey mullet (\textit{Mugil cephalus} ) was reported in the delta region of the Karteros River, Crete, Greece. The investigation identified the primary cause of mortality as severe parasitic infections, specifically from the protozoan parasites Amyloodinium ocellatum and Trichodina sp., both of which heavily affected the gills of the fish. Concurrently, poor water quality in the area, likely due to reduced water volume and limited water renewal, created favourable conditions for parasite proliferation while weakening the fish's natural defences. This event raised concerns due to the location of the fish kill in the Karteros River delta, a highly protected area designated as a small island wetland.}, keywords = {}, pubstate = {published}, tppubtype = {article} } ABSTRACT In August 2024, a significant fish kill involving hundreds of flathead grey mullet (Mugil cephalus ) was reported in the delta region of the Karteros River, Crete, Greece. The investigation identified the primary cause of mortality as severe parasitic infections, specifically from the protozoan parasites Amyloodinium ocellatum and Trichodina sp., both of which heavily affected the gills of the fish. Concurrently, poor water quality in the area, likely due to reduced water volume and limited water renewal, created favourable conditions for parasite proliferation while weakening the fish's natural defences. This event raised concerns due to the location of the fish kill in the Karteros River delta, a highly protected area designated as a small island wetland. |
Brecko, Jonathan; Mathys, Aurore; Chatzinikolaou, Eva; Keklikoglou, Kleoniki; Blettery, Jonathan; Green, Laura; Musson, Alicia; Paton, Alan; Phillips, Sarah; Bastir, Markus; Wiltschke, Karin; Rainer, Heimo; Kroh, Andreas; Haston, Elspeth; Semal, Patrick DIGIT-KEY: an aid towards uniform 2D+ and 3D digitisation techniques within natural history collections Journal Article European Journal of Taxonomy, 976 , 2025, ISSN: 2118-9773. @article{brecko_digit-key_2025, title = {DIGIT-KEY: an aid towards uniform 2D+ and 3D digitisation techniques within natural history collections}, author = {Jonathan Brecko and Aurore Mathys and Eva Chatzinikolaou and Kleoniki Keklikoglou and Jonathan Blettery and Laura Green and Alicia Musson and Alan Paton and Sarah Phillips and Markus Bastir and Karin Wiltschke and Heimo Rainer and Andreas Kroh and Elspeth Haston and Patrick Semal}, url = {https://europeanjournaloftaxonomy.eu/index.php/ejt/article/view/2797 /wp-content/uploads/2025/03/2025-Brecko-EuJTax-9.pdf}, doi = {10.5852/ejt.2025.976.2797}, issn = {2118-9773}, year = {2025}, date = {2025-03-04}, urldate = {2025-03-04}, journal = {European Journal of Taxonomy}, volume = {976}, abstract = {Natural History institutes hold an immense number of specimens and artefacts. For years these collections were not accessible online, remaining inaccessible to researchers from far away and hidden from the general public. Large digitisation projects and cross-institutional agreements aim to bring their collections into the digital era, such as the SYNTHESYS+ project and the Distributed System of Scientific Collections (DiSSCo) Research Infrastructure. As specimens are 3D physical objects with different characteristics many techniques are available to 3D digitise them. For inexperienced users this can be quite overwhelming. Which techniques are already well tested in other institutions and are suitable for a specific specimen or collection? To investigate this, we have set up a dichotomous identification key for digitisation techniques: DIGIT-KEY, (https://digit.naturalheritage.be/digit-key). For each technique, examples used in SYNTHESYS+ Institutions are visualised and training manuals provided. All information can be easily updated and representatives can be contacted if necessary to request more information about a certain technique. This key can be helpful to achieve comparable results across institutions when digitising collections on demand in future DiSSCo research initiatives coordinated through the European Loans and Visits System (ELViS) for Virtual and Transnational Access.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Natural History institutes hold an immense number of specimens and artefacts. For years these collections were not accessible online, remaining inaccessible to researchers from far away and hidden from the general public. Large digitisation projects and cross-institutional agreements aim to bring their collections into the digital era, such as the SYNTHESYS+ project and the Distributed System of Scientific Collections (DiSSCo) Research Infrastructure. As specimens are 3D physical objects with different characteristics many techniques are available to 3D digitise them. For inexperienced users this can be quite overwhelming. Which techniques are already well tested in other institutions and are suitable for a specific specimen or collection? To investigate this, we have set up a dichotomous identification key for digitisation techniques: DIGIT-KEY, (https://digit.naturalheritage.be/digit-key). For each technique, examples used in SYNTHESYS+ Institutions are visualised and training manuals provided. All information can be easily updated and representatives can be contacted if necessary to request more information about a certain technique. This key can be helpful to achieve comparable results across institutions when digitising collections on demand in future DiSSCo research initiatives coordinated through the European Loans and Visits System (ELViS) for Virtual and Transnational Access. |
Grigorakis, Kriton; Kogiannou, Dimitra; Kotsiri, Mado; Kleidas, Ioannis; Mello, Paulo De H; Habiballah, Salaheldeen; Alshaikhi, Ali; Alhafedh, Youssef S; Mohamed, Asaad H W Freshness and Spoilage Patterns of Wild and Farmed Tropical Fish Species with Major Commercial Importance Originating from Saudi Arabian Waters Journal Article Foods, 14 (4), pp. 690, 2025, ISSN: 2304-8158. @article{grigorakis_freshness_2025, title = {Freshness and Spoilage Patterns of Wild and Farmed Tropical Fish Species with Major Commercial Importance Originating from Saudi Arabian Waters}, author = {Kriton Grigorakis and Dimitra Kogiannou and Mado Kotsiri and Ioannis Kleidas and Paulo H De Mello and Salaheldeen Habiballah and Ali Alshaikhi and Youssef S Alhafedh and Asaad H W Mohamed}, url = {https://www.mdpi.com/2304-8158/14/4/690 /wp-content/uploads/2025/02/2025-Grigorakis-foods-8.pdf}, doi = {10.3390/foods14040690}, issn = {2304-8158}, year = {2025}, date = {2025-02-24}, urldate = {2025-02-24}, journal = {Foods}, volume = {14}, number = {4}, pages = {690}, abstract = {Ice-stored farmed barramundi (Lates calcarifer), snubnose pompano (Trachinotus blochii) and sobaity bream (Sparidentex hasta), as well as wild-caught cobia (Rachycentron canadum), coral trout (Plectropomus leopardus), giant trevally (Caranx ignobilis), milkfish (Chanos chanos) and mangrove red snapper (Lutjanus argentimaculatus), were compared for their freshness/spoilage using sensory, chemical and microbiological methods. Quality Index Method schemes were developed to determine alterations in the sensory freshness. The shelf lives ranged from 8 (coral trout) to 18 days (sobaity bream). The farmed species always exhibited a significantly longer shelf life than the wild-caught species. The adenosine triphosphate (ATP) breakdown followed different patterns in the studied species. The K-values at the time of sensory rejection ranged from 30 to 80% depending on the species, while the microbial load reached or exceeded a level of 6 log cfu/g. Although the shelf life duration was dependent on the origin of the fish (wild or farmed), the ATP breakdown scheme, as well as the K-values and microbial loads at the time of rejection, were species-dependent and independent of the origin.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Ice-stored farmed barramundi (Lates calcarifer), snubnose pompano (Trachinotus blochii) and sobaity bream (Sparidentex hasta), as well as wild-caught cobia (Rachycentron canadum), coral trout (Plectropomus leopardus), giant trevally (Caranx ignobilis), milkfish (Chanos chanos) and mangrove red snapper (Lutjanus argentimaculatus), were compared for their freshness/spoilage using sensory, chemical and microbiological methods. Quality Index Method schemes were developed to determine alterations in the sensory freshness. The shelf lives ranged from 8 (coral trout) to 18 days (sobaity bream). The farmed species always exhibited a significantly longer shelf life than the wild-caught species. The adenosine triphosphate (ATP) breakdown followed different patterns in the studied species. The K-values at the time of sensory rejection ranged from 30 to 80% depending on the species, while the microbial load reached or exceeded a level of 6 log cfu/g. Although the shelf life duration was dependent on the origin of the fish (wild or farmed), the ATP breakdown scheme, as well as the K-values and microbial loads at the time of rejection, were species-dependent and independent of the origin. |
Carlot, J; Galobart, C; Gómez-Gras, D; Santamaría, J; Golo, R; Sini, M; Cebrian, E; Gerovasileiou, V; Ponti, M; Turicchia, E; Comeau, S; Rilov, G; Tamburello, L; Mantas, Pulido T; Cerrano, C; Ledoux, J B; Gattuso, J -P; Ramirez-Calero, S; Millan, L; Montefalcone, M; Katsanevakis, S; Bensoussan, N; Garrabou, J; Teixidó, N Vulnerability of benthic trait diversity across the Mediterranean Sea following mass mortality events Journal Article Nature Communications, 16 (1), pp. 1571, 2025, ISSN: 2041-1723. @article{carlot_vulnerability_2025, title = {Vulnerability of benthic trait diversity across the Mediterranean Sea following mass mortality events}, author = {J Carlot and C Galobart and D Gómez-Gras and J Santamaría and R Golo and M Sini and E Cebrian and V Gerovasileiou and M Ponti and E Turicchia and S Comeau and G Rilov and L Tamburello and T Pulido Mantas and C Cerrano and J B Ledoux and J -P Gattuso and S Ramirez-Calero and L Millan and M Montefalcone and S Katsanevakis and N Bensoussan and J Garrabou and N Teixidó}, url = {https://www.nature.com/articles/s41467-025-55949-0 /wp-content/uploads/2025/02/2025-Carlot-Nat-Comm-7.pdf}, doi = {10.1038/s41467-025-55949-0}, issn = {2041-1723}, year = {2025}, date = {2025-02-21}, urldate = {2025-02-20}, journal = {Nature Communications}, volume = {16}, number = {1}, pages = {1571}, abstract = {Abstract Unraveling the functional future of marine ecosystems amid global change poses a pressing challenge. This is particularly critical in the Mediterranean Sea, which is highly impacted by global and local drivers. Utilizing extensive mass mortality events (MMEs) datasets spanning from 1986 to 2020 across the Mediterranean Sea, we investigated the trait vulnerability of benthic species that suffered from MMEs induced by nine distinct mortality drivers. By analyzing changes in ten ecological traits across 389 benthic species—constituting an extensive compendium of Mediterranean ecological traits to date—we identified 228 functional entities (FEs), defined as groups of species sharing the same trait values. Our findings indicate that of these 55 FEs were impacted by MMEs, accentuating a heightened vulnerability within specific trait categories. Notably, more than half of the mortality records showed severe impacts on calcifying and larger species with slower growth which mostly account for tree-like and massive forms. Altogether, we highlight that 29 FEs suffered extreme mortality, leading to a maximum increase of 19.1% of the global trait volume vulnerability over 35 years. We also reveal that 10.8% of the trait volume may have been temporarily lost over the last five years, emphasizing the risk of a rapid ecological transformation in the Mediterranean Sea.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Unraveling the functional future of marine ecosystems amid global change poses a pressing challenge. This is particularly critical in the Mediterranean Sea, which is highly impacted by global and local drivers. Utilizing extensive mass mortality events (MMEs) datasets spanning from 1986 to 2020 across the Mediterranean Sea, we investigated the trait vulnerability of benthic species that suffered from MMEs induced by nine distinct mortality drivers. By analyzing changes in ten ecological traits across 389 benthic species—constituting an extensive compendium of Mediterranean ecological traits to date—we identified 228 functional entities (FEs), defined as groups of species sharing the same trait values. Our findings indicate that of these 55 FEs were impacted by MMEs, accentuating a heightened vulnerability within specific trait categories. Notably, more than half of the mortality records showed severe impacts on calcifying and larger species with slower growth which mostly account for tree-like and massive forms. Altogether, we highlight that 29 FEs suffered extreme mortality, leading to a maximum increase of 19.1% of the global trait volume vulnerability over 35 years. We also reveal that 10.8% of the trait volume may have been temporarily lost over the last five years, emphasizing the risk of a rapid ecological transformation in the Mediterranean Sea. |
Daraghmeh, Nauras; Exter, Katrina; Pagnier, Justine; Balazy, Piotr; Cancio, Ibon; Chatzigeorgiou, Giorgos; Chatzinikolaou, Eva; Chelchowski, Maciej; Chrismas, Nathan Alexis Mitchell; Comtet, Thierry; Dailianis, Thanos; Deneudt, Klaas; Cerio, Oihane Diaz De; Digenis, Markos; Gerovasileiou, Vasilis; González, José; Kauppi, Laura; Kristoffersen, Jon Bent; Kukliński, Piotr; Lasota, Rafał; Levy, Liraz; Małachowicz, Magdalena; Mavrič, Borut; Mortelmans, Jonas; Paredes, Estefania; Poćwierz‐Kotus, Anita; Reiss, Henning; Santi, Ioulia; Sarafidou, Georgia; Skouradakis, Grigorios; Solbakken, Jostein; Staehr, Peter A U; Tajadura, Javier; Thyrring, Jakob; Troncoso, Jesus S; Vernadou, Emmanouela; Viard, Frederique; Zafeiropoulos, Haris; Zbawicka, Małgorzata; Pavloudi, Christina; Obst, Matthias A Long-Term Ecological Research Data Set From the Marine Genetic Monitoring Program ARMS-MBON 2018–2020 Journal Article Molecular Ecology Resources, pp. e14073, 2025, ISSN: 1755-098X, 1755-0998. @article{daraghmeh_longterm_2025, title = {A Long-Term Ecological Research Data Set From the Marine Genetic Monitoring Program ARMS-MBON 2018–2020}, author = {Nauras Daraghmeh and Katrina Exter and Justine Pagnier and Piotr Balazy and Ibon Cancio and Giorgos Chatzigeorgiou and Eva Chatzinikolaou and Maciej Chelchowski and Nathan Alexis Mitchell Chrismas and Thierry Comtet and Thanos Dailianis and Klaas Deneudt and Oihane Diaz De Cerio and Markos Digenis and Vasilis Gerovasileiou and José González and Laura Kauppi and Jon Bent Kristoffersen and Piotr Kukliński and Rafał Lasota and Liraz Levy and Magdalena Małachowicz and Borut Mavrič and Jonas Mortelmans and Estefania Paredes and Anita Poćwierz‐Kotus and Henning Reiss and Ioulia Santi and Georgia Sarafidou and Grigorios Skouradakis and Jostein Solbakken and Peter A U Staehr and Javier Tajadura and Jakob Thyrring and Jesus S Troncoso and Emmanouela Vernadou and Frederique Viard and Haris Zafeiropoulos and Małgorzata Zbawicka and Christina Pavloudi and Matthias Obst}, url = {https://onlinelibrary.wiley.com/doi/10.1111/1755-0998.14073 /wp-content/uploads/2025/02/2025-Daraghmeh-MolEcoRes-6.pdf}, doi = {10.1111/1755-0998.14073}, issn = {1755-098X, 1755-0998}, year = {2025}, date = {2025-02-05}, urldate = {2025-02-05}, journal = {Molecular Ecology Resources}, pages = {e14073}, abstract = {ABSTRACT Molecular methods such as DNA/eDNA metabarcoding have emerged as useful tools to document the biodiversity of complex communities over large spatio‐temporal scales. We established an international Marine Biodiversity Observation Network (ARMS‐MBON) combining standardised sampling using autonomous reef monitoring structures (ARMS) with metabarcoding for genetic monitoring of marine hard‐bottom benthic communities. Here, we present the data of our first sampling campaign comprising 56 ARMS units deployed in 2018–2019 and retrieved in 2018–2020 across 15 observatories along the coasts of Europe and adjacent regions. We describe the open‐access data set (image, genetic and metadata) and explore the genetic data to show its potential for marine biodiversity monitoring and ecological research. Our analysis shows that ARMS recovered more than 60 eukaryotic phyla capturing diversity of up to textasciitilde5500 amplicon sequence variants and textasciitilde1800 operational taxonomic units, and up to textasciitilde250 and textasciitilde50 species per observatory using the cytochrome c oxidase subunit I (COI) and 18S rRNA marker genes, respectively. Further, ARMS detected threatened, vulnerable and non‐indigenous species often targeted in biological monitoring. We show that while deployment duration does not drive diversity estimates, sampling effort and sequencing depth across observatories do. We recommend that ARMS should be deployed for at least 3–6 months during the main growth season to use resources as efficiently as possible and that post‐sequencing curation is applied to enable statistical comparison of spatio‐temporal entities. We suggest that ARMS should be used in biological monitoring programs and long‐term ecological research and encourage the adoption of our ARMS‐MBON protocols.}, keywords = {}, pubstate = {published}, tppubtype = {article} } ABSTRACT Molecular methods such as DNA/eDNA metabarcoding have emerged as useful tools to document the biodiversity of complex communities over large spatio‐temporal scales. We established an international Marine Biodiversity Observation Network (ARMS‐MBON) combining standardised sampling using autonomous reef monitoring structures (ARMS) with metabarcoding for genetic monitoring of marine hard‐bottom benthic communities. Here, we present the data of our first sampling campaign comprising 56 ARMS units deployed in 2018–2019 and retrieved in 2018–2020 across 15 observatories along the coasts of Europe and adjacent regions. We describe the open‐access data set (image, genetic and metadata) and explore the genetic data to show its potential for marine biodiversity monitoring and ecological research. Our analysis shows that ARMS recovered more than 60 eukaryotic phyla capturing diversity of up to textasciitilde5500 amplicon sequence variants and textasciitilde1800 operational taxonomic units, and up to textasciitilde250 and textasciitilde50 species per observatory using the cytochrome c oxidase subunit I (COI) and 18S rRNA marker genes, respectively. Further, ARMS detected threatened, vulnerable and non‐indigenous species often targeted in biological monitoring. We show that while deployment duration does not drive diversity estimates, sampling effort and sequencing depth across observatories do. We recommend that ARMS should be deployed for at least 3–6 months during the main growth season to use resources as efficiently as possible and that post‐sequencing curation is applied to enable statistical comparison of spatio‐temporal entities. We suggest that ARMS should be used in biological monitoring programs and long‐term ecological research and encourage the adoption of our ARMS‐MBON protocols. |
Chen, I-Hao; Georgopoulou, Dimitra G; Ebbesson, Lars O E; Voskakis, Dimitris; Munthe-Kaas, Antonella Zanna; Papandroulakis, Nikos Acoustic tags versus camera—a case study on feeding behaviour of European seabass in sea cages Journal Article Frontiers in Marine Science, 11 , pp. 1497336, 2025, ISSN: 2296-7745. @article{chen_acoustic_2025, title = {Acoustic tags versus camera—a case study on feeding behaviour of European seabass in sea cages}, author = {I-Hao Chen and Dimitra G Georgopoulou and Lars O E Ebbesson and Dimitris Voskakis and Antonella Zanna Munthe-Kaas and Nikos Papandroulakis}, url = {https://www.frontiersin.org/articles/10.3389/fmars.2024.1497336/full https://imbbc.hcmr.gr/wp-content/uploads/2025/01/2025-Chen-fmars-5.pdf}, doi = {10.3389/fmars.2024.1497336}, issn = {2296-7745}, year = {2025}, date = {2025-01-30}, urldate = {2025-01-30}, journal = {Frontiers in Marine Science}, volume = {11}, pages = {1497336}, abstract = {Intoduction With the expansion of the aquaculture industry, the need arises for scalable, reliable, and robust methods to assess fish behaviour in sea cages to guide operational management, which includes feeding optimisation and welfare assessments. Fish cage monitoring utilising either acoustic transmitters or underwater cameras is well-studied. However, the relationship between those two different measurement types seems to have not been explored, nor have they been evaluated together in one experimental site. Methods In our 1-month study, we compared the activity of 14 sentinel fish and the artificial intelligence (AI)-inferred speed of individuals from the European seabass ( Dicentrarchus labrax ) sea cage population in three feeding trials. Comparisons include a maximum activity comparison using persistent peaks, fish behavioural pattern establishment and retention, and periodical behavioural patterns. Results Our results demonstrate that under certain circumstances, both technologies are interchangeable from the perspective of persistent peaks and periodicity, but complementary when it comes to behaviour analysis such as food anticipatory behaviour (FAB). Discussion We anticipate that our findings will stimulate advances where multiple sensor types are in use to achieve a more holistic understanding of fish behaviour in the aquaculture sector using underwater technologies.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Intoduction With the expansion of the aquaculture industry, the need arises for scalable, reliable, and robust methods to assess fish behaviour in sea cages to guide operational management, which includes feeding optimisation and welfare assessments. Fish cage monitoring utilising either acoustic transmitters or underwater cameras is well-studied. However, the relationship between those two different measurement types seems to have not been explored, nor have they been evaluated together in one experimental site. Methods In our 1-month study, we compared the activity of 14 sentinel fish and the artificial intelligence (AI)-inferred speed of individuals from the European seabass ( Dicentrarchus labrax ) sea cage population in three feeding trials. Comparisons include a maximum activity comparison using persistent peaks, fish behavioural pattern establishment and retention, and periodical behavioural patterns. Results Our results demonstrate that under certain circumstances, both technologies are interchangeable from the perspective of persistent peaks and periodicity, but complementary when it comes to behaviour analysis such as food anticipatory behaviour (FAB). Discussion We anticipate that our findings will stimulate advances where multiple sensor types are in use to achieve a more holistic understanding of fish behaviour in the aquaculture sector using underwater technologies. |
Oikonomou, Stavroula; Tasiouli, Katerina; Tsaparis, Dimitrios; Manousaki, Tereza; Vallecillos, Antonio; Oikonomaki, Katerina; Tzokas, Konstantinos; Katribouzas, Nikolaos; Batargias, Costas; Chatziplis, Dimitrios; Tsigenopoulos, Costas S Genomic evaluation for body weight, length and growth estimates in meagre Argyrosomus regius Journal Article Aquaculture, 595 , pp. 741622, 2025, ISSN: 00448486. @article{oikonomou_genomic_2025, title = {Genomic evaluation for body weight, length and growth estimates in meagre Argyrosomus regius}, author = {Stavroula Oikonomou and Katerina Tasiouli and Dimitrios Tsaparis and Tereza Manousaki and Antonio Vallecillos and Katerina Oikonomaki and Konstantinos Tzokas and Nikolaos Katribouzas and Costas Batargias and Dimitrios Chatziplis and Costas S Tsigenopoulos}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848624010846 /wp-content/uploads/2025/01/2025-Oikonomou-AQUA-3.pdf}, doi = {10.1016/j.aquaculture.2024.741622}, issn = {00448486}, year = {2025}, date = {2025-01-24}, urldate = {2025-01-24}, journal = {Aquaculture}, volume = {595}, pages = {741622}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Lancerotto, Stefano; Fakriadis, Ioannis; Papadaki, Maria; Giménez, Ignacio; Genovés, José Vicente Roig; Mylonas, Constantinos C Aquaculture, 594 , pp. 741401, 2025, ISSN: 00448486. @article{lancerotto_overcoming_2025, title = {Overcoming dysfunctional gametogenesis in hatchery-produced greater amberjack, Seriola dumerili using recombinant gonadotropins, and spawning induction using gonadotropin releasing hormone agonist-loaded implants}, author = {Stefano Lancerotto and Ioannis Fakriadis and Maria Papadaki and Ignacio Giménez and José Vicente Roig Genovés and Constantinos C Mylonas}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848624008627 https://imbbc.hcmr.gr/wp-content/uploads/2025/01/2025-Lancerotto-AQUA-2.pdf}, doi = {10.1016/j.aquaculture.2024.741401}, issn = {00448486}, year = {2025}, date = {2025-01-21}, urldate = {2025-01-21}, journal = {Aquaculture}, volume = {594}, pages = {741401}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Dimitriadis, C; Marampouti, C; Calò, A; Franco, Di A; Giakoumi, S; Franco, Di E; Lorenzo, Di M; Gerovasileiou, V; Guidetti, P; Pey, A; Sini, M; Sourbès, L Marine Environmental Research, 193 , pp. 106293, 2025, ISSN: 01411136. @article{dimitriadis_evaluating_2024, title = {Evaluating the long term effectiveness of a Mediterranean marine protected area to tackle the effects of invasive and range expanding herbivorous fish on rocky reefs}, author = {C Dimitriadis and C Marampouti and A Calò and A Di Franco and S Giakoumi and E Di Franco and M Di Lorenzo and V Gerovasileiou and P Guidetti and A Pey and M Sini and L Sourbès}, url = {https://linkinghub.elsevier.com/retrieve/pii/S014111362300421X /wp-content/uploads/2025/06/PREPRINT-Dimitriadis-et-al-2024.pdf}, doi = {10.1016/j.marenvres.2023.106293}, issn = {01411136}, year = {2025}, date = {2025-01-13}, urldate = {2025-06-12}, journal = {Marine Environmental Research}, volume = {193}, pages = {106293}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Kourkouta, Chara; Tsipourlianos, Andreas; Papandroulakis, Nikos; Moutou, Katerina A; Koumoundouros, George Ray‐Resorption Syndrome in European Seabass, textitDicentrarchus labrax (Linnaeus, 1758) Journal Article Journal of Fish Diseases, 48 (2), pp. e14042, 2025, ISSN: 0140-7775, 1365-2761. @article{kourkouta_rayresorption_2025, title = {Ray‐Resorption Syndrome in European Seabass, textitDicentrarchus labrax (Linnaeus, 1758)}, author = {Chara Kourkouta and Andreas Tsipourlianos and Nikos Papandroulakis and Katerina A Moutou and George Koumoundouros}, url = {https://onlinelibrary.wiley.com/doi/10.1111/jfd.14042 https://imbbc.hcmr.gr/wp-content/uploads/2025/01/2025-Kourkouta-JFD-4.pdf}, doi = {10.1111/jfd.14042}, issn = {0140-7775, 1365-2761}, year = {2025}, date = {2025-01-13}, urldate = {2025-01-13}, journal = {Journal of Fish Diseases}, volume = {48}, number = {2}, pages = {e14042}, abstract = {ABSTRACT Fin abnormalities are common in reared fish. They mainly consist of partial to complete lack of rays and severe abnormalities of fin‐supporting skeletal elements, which develop during the larval stage, up to the completion of fin skeleton ontogeny. This study reports a new abnormal condition, ray‐resorption syndrome (RSS), which developed after the completion of fin ontogeny in initially normal European seabass larvae, leading to extensive loss of fin rays. At 49 days post‐fertilisation (dpf) (15 mm standard length, SL) all fish presented normal fins. However, nineteen days later (21 mm SL), significant fin damage, characterised by ray loss or fractures, was observed in all studied populations. The dorsal fin was most affected (55%–84%), followed by the pelvic (27%–53%) and anal fins (7%–17%). Microscopically, multiple non‐mineralised areas resembling resorption lacunae were evident along all fin lepidotrichia. By 98 dpf (40 mm SL), the fin‐ray loss had advanced, reaching its higher frequency in the dorsal (74%–83%) and caudal fins (71%–94%). Gene expression analysis revealed a significant upregulation of bglap (osteoblast maturation marker), acp5a (osteoclast maturation marker) and mmp13a (extracellular‐matrix remodelling marker) in RSS specimens. The results are discussed in respect of the possible causative factors of RSS.}, keywords = {}, pubstate = {published}, tppubtype = {article} } ABSTRACT Fin abnormalities are common in reared fish. They mainly consist of partial to complete lack of rays and severe abnormalities of fin‐supporting skeletal elements, which develop during the larval stage, up to the completion of fin skeleton ontogeny. This study reports a new abnormal condition, ray‐resorption syndrome (RSS), which developed after the completion of fin ontogeny in initially normal European seabass larvae, leading to extensive loss of fin rays. At 49 days post‐fertilisation (dpf) (15 mm standard length, SL) all fish presented normal fins. However, nineteen days later (21 mm SL), significant fin damage, characterised by ray loss or fractures, was observed in all studied populations. The dorsal fin was most affected (55%–84%), followed by the pelvic (27%–53%) and anal fins (7%–17%). Microscopically, multiple non‐mineralised areas resembling resorption lacunae were evident along all fin lepidotrichia. By 98 dpf (40 mm SL), the fin‐ray loss had advanced, reaching its higher frequency in the dorsal (74%–83%) and caudal fins (71%–94%). Gene expression analysis revealed a significant upregulation of bglap (osteoblast maturation marker), acp5a (osteoclast maturation marker) and mmp13a (extracellular‐matrix remodelling marker) in RSS specimens. The results are discussed in respect of the possible causative factors of RSS. |
Bušelić, Ivana; Trumbić, Željka; Hrabar, Jerko; Lepen-Pleić, Ivana; Šegvić-Bubić, Tanja; Kaitetzidou, Elisavet; Tibaldi, Emilio; Bočina, Ivana; Grubišić, Leon; Sarropoulou, Elena 594 , pp. 741385, 2025, ISSN: 00448486. @article{buselic_unravelling_2025, title = {Unravelling the intricate language of fish guts: Impact of plant-based vs. plant-insect-poultry-based diets on intestinal pathways in European seabass}, author = {Ivana Bušelić and Željka Trumbić and Jerko Hrabar and Ivana Lepen-Pleić and Tanja Šegvić-Bubić and Elisavet Kaitetzidou and Emilio Tibaldi and Ivana Bočina and Leon Grubišić and Elena Sarropoulou}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848624008469 https://imbbc.hcmr.gr/wp-content/uploads/2025/01/2025-Buselic-AQUA-1.pdf}, doi = {10.1016/j.aquaculture.2024.741385}, issn = {00448486}, year = {2025}, date = {2025-01-08}, urldate = {2025-01-08}, volume = {594}, pages = {741385}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Pavloudi, Christina; Santi, Ioulia; Azua, Iñigo; Baña, Zuriñe; Bastianini, Mauro; Belser, Caroline; Bilbao, Jone; Bitz-Thorsen, Julie; Broudin, Caroline; Camusat, Mathieu; Cancio, Ibon; Caray-Counil, Louis; Casotti, Raffaella; Castel, Jade; Comtet, Thierry; Cox, Cymon; Daguin, Claire; Cerio, Oihane Díaz De; Exter, Katrina; Fauvelot, Cécile; Frada, Miguel; Galand, Pierre; Garczarek, Laurence; Fernández, Jose González; Guillou, Laure; Hablützel, Pascal; Heynderickx, Hanneloor; Houbin, Céline; Kervella, Anne; Krystallas, Apostolos; Lagaisse, Rune; Laroquette, Arnaud; Lescure, Lyvia; Lopes, Eva; Loulakaki, Melina; Louro, Bruno; Magalhaes, Catarina; Maidanou, Maria; Margiotta, Francesca; Montresor, Marina; Not, Fabrice; Paredes, Estefanía; Percopo, Isabella; Péru, Erwan; Poulain, Julie; Præbel, Kim; Rigaut-Jalabert, Fabienne; Romac, Sarah; Stavroulaki, Melanthia; Troncoso, Jesús Souza; Thiébaut, Eric; Thomas, Wilfried; Tkacz, Andrzej; Trano, Anna Chiara; Wincker, Patrick; Pade, Nicolas First release of the European marine omics biodiversity observation network (EMO BON) shotgun metagenomics data from water and sediment samples Journal Article Biodiversity Data Journal, 13 , pp. e143585, 2025, ISSN: 1314-2828, 1314-2836. @article{pavloudi_first_2025, title = {First release of the European marine omics biodiversity observation network (EMO BON) shotgun metagenomics data from water and sediment samples}, author = {Christina Pavloudi and Ioulia Santi and Iñigo Azua and Zuriñe Baña and Mauro Bastianini and Caroline Belser and Jone Bilbao and Julie Bitz-Thorsen and Caroline Broudin and Mathieu Camusat and Ibon Cancio and Louis Caray-Counil and Raffaella Casotti and Jade Castel and Thierry Comtet and Cymon Cox and Claire Daguin and Oihane Díaz De Cerio and Katrina Exter and Cécile Fauvelot and Miguel Frada and Pierre Galand and Laurence Garczarek and Jose González Fernández and Laure Guillou and Pascal Hablützel and Hanneloor Heynderickx and Céline Houbin and Anne Kervella and Apostolos Krystallas and Rune Lagaisse and Arnaud Laroquette and Lyvia Lescure and Eva Lopes and Melina Loulakaki and Bruno Louro and Catarina Magalhaes and Maria Maidanou and Francesca Margiotta and Marina Montresor and Fabrice Not and Estefanía Paredes and Isabella Percopo and Erwan Péru and Julie Poulain and Kim Præbel and Fabienne Rigaut-Jalabert and Sarah Romac and Melanthia Stavroulaki and Jesús Souza Troncoso and Eric Thiébaut and Wilfried Thomas and Andrzej Tkacz and Anna Chiara Trano and Patrick Wincker and Nicolas Pade}, url = {https://bdj.pensoft.net/article/143585/ /wp-content/uploads/2025/04/2025-Pavloudi-BDJ-17.pdf}, doi = {10.3897/BDJ.13.e143585}, issn = {1314-2828, 1314-2836}, year = {2025}, date = {2025-01-01}, urldate = {2025-04-03}, journal = {Biodiversity Data Journal}, volume = {13}, pages = {e143585}, abstract = {The European Marine Omics Biodiversity Observation Network (EMO BON) is an initiative of the European Marine Biological Resource Centre (EMBRC) to establish a persistent genomic observatory amongst designated European coastal marine sites, sharing the same protocols for sampling and data curation. Environmental samples are collected from the water column and, at some sites, soft sediments and hard substrates (Autonomous Reef Monitoring Structures - ARMS), together with a set of mandatory and discretionary metadata (including Essential Ocean Variables - EOVs). Samples are collected following standardised protocols at regular and specified intervals and sequenced in large six-monthly batches at a centralised sequencing facility. The use of standard operating procedures (SOPs) during data collection, library preparation and sequencing aims to provide uniformity amongst the data collected from the sites. Coupled with strict adherence to open and FAIR (Findable, Accessible, Interoperable, Reusable) data principles, this ensures maximum comparability amongst samples and enhances reusability and interoperability of the data with other data sources. The observatory network was launched in June 2021, when the first sampling campaign took place.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The European Marine Omics Biodiversity Observation Network (EMO BON) is an initiative of the European Marine Biological Resource Centre (EMBRC) to establish a persistent genomic observatory amongst designated European coastal marine sites, sharing the same protocols for sampling and data curation. Environmental samples are collected from the water column and, at some sites, soft sediments and hard substrates (Autonomous Reef Monitoring Structures - ARMS), together with a set of mandatory and discretionary metadata (including Essential Ocean Variables - EOVs). Samples are collected following standardised protocols at regular and specified intervals and sequenced in large six-monthly batches at a centralised sequencing facility. The use of standard operating procedures (SOPs) during data collection, library preparation and sequencing aims to provide uniformity amongst the data collected from the sites. Coupled with strict adherence to open and FAIR (Findable, Accessible, Interoperable, Reusable) data principles, this ensures maximum comparability amongst samples and enhances reusability and interoperability of the data with other data sources. The observatory network was launched in June 2021, when the first sampling campaign took place. |
2024 |
Voskakis, Dimitris; Kelasidi, Eleni; Papandroulakis, Nikos Design and Development of Underwater Robotic Arm for Automated Camera Calibration for Aquatic Environment Inproceedings 2024 10th International Conference on Automation, Robotics and Applications (ICARA), pp. 50–56, IEEE, Athens, Greece, 2024, ISBN: 9798350394245. @inproceedings{voskakis_design_2024, title = {Design and Development of Underwater Robotic Arm for Automated Camera Calibration for Aquatic Environment}, author = {Dimitris Voskakis and Eleni Kelasidi and Nikos Papandroulakis}, url = {https://ieeexplore.ieee.org/document/10552983/ https://imbbc.hcmr.gr/wp-content/uploads/2025/01/ICARA-2024-Voskakis-pre-print.pdf}, doi = {10.1109/ICARA60736.2024.10552983}, isbn = {9798350394245}, year = {2024}, date = {2024-12-31}, urldate = {2025-01-10}, booktitle = {2024 10th International Conference on Automation, Robotics and Applications (ICARA)}, pages = {50--56}, publisher = {IEEE}, address = {Athens, Greece}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Mladineo, Ivona; Hrabar, Jerko; Trumbić, Željka; Rasouli-Dogaheh, Somayeh; Beraldo, Paola; Rigos, George; Palenzuela, Oswado; Sitjà-Bobadilla, Ariadna Aquaculture, 598 , pp. 741997, 2024, ISSN: 00448486. @article{mladineo_mediterranean-wide_2025, title = {Mediterranean-wide transfer of the polyopisthocotylean Sparicotyle chrysophrii between wild sparids and farmed gilthead seabream (Sparus aurata) inferred by ddRAD loci}, author = {Ivona Mladineo and Jerko Hrabar and Željka Trumbić and Somayeh Rasouli-Dogaheh and Paola Beraldo and George Rigos and Oswado Palenzuela and Ariadna Sitjà-Bobadilla}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848624014595 https://imbbc.hcmr.gr/wp-content/uploads/2025/01/2024-Mladineo...Rigos-et-al-pre-print-48.pdf}, doi = {10.1016/j.aquaculture.2024.741997}, issn = {00448486}, year = {2024}, date = {2024-12-30}, urldate = {2025-01-08}, journal = {Aquaculture}, volume = {598}, pages = {741997}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Livanou, Eleni; Sauzède, Raphaëlle; Psarra, Stella; Mandalakis, Manolis; Dall’Olmo, Giorgio; Brewin, Robert J W; Raitsos, Dionysios E Evaluating MULTIOBS Chlorophyll-a with Ground-Truth Observations in the Eastern Mediterranean Sea Journal Article Remote Sensing, 16 (24), pp. 4705, 2024, ISSN: 2072-4292. @article{livanou_evaluating_2024, title = {Evaluating MULTIOBS Chlorophyll-a with Ground-Truth Observations in the Eastern Mediterranean Sea}, author = {Eleni Livanou and Raphaëlle Sauzède and Stella Psarra and Manolis Mandalakis and Giorgio Dall’Olmo and Robert J W Brewin and Dionysios E Raitsos}, url = {https://www.mdpi.com/2072-4292/16/24/4705 /wp-content/uploads/2024/12/2024-Livanou-Remote-Sensing-49.pdf}, doi = {10.3390/rs16244705}, issn = {2072-4292}, year = {2024}, date = {2024-12-23}, urldate = {2024-12-23}, journal = {Remote Sensing}, volume = {16}, number = {24}, pages = {4705}, abstract = {Satellite-derived observations of ocean colour provide continuous data on chlorophyll-a concentration (Chl-a) at global scales but are limited to the ocean’s surface. So far, biogeochemical models have been the only means of generating continuous vertically resolved Chl-a profiles on a regular grid. MULTIOBS is a multi-observations oceanographic dataset that provides depth-resolved biological data based on merged satellite- and Argo-derived in situ hydrological data. This product is distributed by the European Union’s Copernicus Marine Service and offers global multiyear, gridded Chl-a profiles within the ocean’s productive zone at a weekly temporal resolution. MULTIOBS addresses the scarcity of observation-based vertically resolved Chl-a datasets, particularly in less sampled regions like the Eastern Mediterranean Sea (EMS). Here, we conduct an independent evaluation of the MULTIOBS dataset in the oligotrophic waters of the EMS using in situ Chl-a profiles. Our analysis shows that this product accurately and precisely retrieves Chl-a across depths, with a slight 1% overestimation and an observed 1.5-fold average deviation between in situ data and MULTIOBS estimates. The deep chlorophyll maximum (DCM) is adequately estimated by MULTIOBS both in terms of positioning (root mean square error}, keywords = {}, pubstate = {published}, tppubtype = {article} } Satellite-derived observations of ocean colour provide continuous data on chlorophyll-a concentration (Chl-a) at global scales but are limited to the ocean’s surface. So far, biogeochemical models have been the only means of generating continuous vertically resolved Chl-a profiles on a regular grid. MULTIOBS is a multi-observations oceanographic dataset that provides depth-resolved biological data based on merged satellite- and Argo-derived in situ hydrological data. This product is distributed by the European Union’s Copernicus Marine Service and offers global multiyear, gridded Chl-a profiles within the ocean’s productive zone at a weekly temporal resolution. MULTIOBS addresses the scarcity of observation-based vertically resolved Chl-a datasets, particularly in less sampled regions like the Eastern Mediterranean Sea (EMS). Here, we conduct an independent evaluation of the MULTIOBS dataset in the oligotrophic waters of the EMS using in situ Chl-a profiles. Our analysis shows that this product accurately and precisely retrieves Chl-a across depths, with a slight 1% overestimation and an observed 1.5-fold average deviation between in situ data and MULTIOBS estimates. The deep chlorophyll maximum (DCM) is adequately estimated by MULTIOBS both in terms of positioning (root mean square error |
Apostolaki, Eugenia T; Lavery, Paul S; Litsi‐Mizan, Victoria; Serrano, Eduard; Inostroza, Karina; Gerakaris, Vasilis; Dailianis, Thanos; Glampedakis, Julius; Holitzki, Tara; Johnson, Erik; Mateo, Miguel A; Serrano, Oscar Patterns of Carbon and Nitrogen Accumulation in Seagrass ( textitPosidonia oceanica ) Meadows of the Eastern Mediterranean Sea Journal Article Journal of Geophysical Research: Biogeosciences, 129 (12), pp. e2024JG008163, 2024, ISSN: 2169-8953, 2169-8961. @article{apostolaki_patterns_2024, title = {Patterns of Carbon and Nitrogen Accumulation in Seagrass ( textitPosidonia oceanica ) Meadows of the Eastern Mediterranean Sea}, author = {Eugenia T Apostolaki and Paul S Lavery and Victoria Litsi‐Mizan and Eduard Serrano and Karina Inostroza and Vasilis Gerakaris and Thanos Dailianis and Julius Glampedakis and Tara Holitzki and Erik Johnson and Miguel A Mateo and Oscar Serrano}, url = {https://agupubs.onlinelibrary.wiley.com/doi/10.1029/2024JG008163 https://imbbc.hcmr.gr/wp-content/uploads/2024/12/2024-Apostolaki-JGR-BiosSci-47-1.pdf}, doi = {10.1029/2024JG008163}, issn = {2169-8953, 2169-8961}, year = {2024}, date = {2024-12-18}, urldate = {2024-12-18}, journal = {Journal of Geophysical Research: Biogeosciences}, volume = {129}, number = {12}, pages = {e2024JG008163}, abstract = {Abstract The variability in stocks and accumulation rates of organic carbon (C org ), nitrogen (N), and carbonate (CaCO 3 ) was studied in fifteen Posidonia oceanica meadows spread throughout the South Aegean Sea (Greece). In addition, the abiotic and biotic drivers determining the pattern of variability in the accumulation rates were assessed by exploring the influence of sediment characteristics, seagrass traits, and environmental settings. The meadows supported on average (±STDEV) 14.6 ± 5.0 kg C org m −2 , 0.47 ± 0.17 kg N m −2 , and 249 ± 210 kg CaCO 3 m −2 in the top meter of their sediments, with mean accumulation rates over the last 500 years of 33.6 ± 23.6 g C org m −2 yr −1 , 1.00 ± 0.62 g N m −2 yr −1 , and 405 ± 336 g CaCO 3 m −2 yr −1 across sites. A redundancy analysis (RDA) explained 70% of the variation in C org , N, and CaCO 3 accumulation rates, with three sediment characteristics (i.e., sediment C org :N and C org :C inorg ratios and P . oceanica contribution to the sediment C org pool) emerging as the primary set of factors shaping the accumulation of matter, followed by seagrass traits (i.e., leaf biomass and rhizome elongation) and environmental variables (i.e., suspended organic matter). The high degree of variability within the region emphasizes the need for fine‐scale assessments to understand the local conditions influencing sequestration. Our findings underscored the critical role of seagrass meadows in carbon and nitrogen sequestration in the region, urging conservation efforts to protect these ecosystems and prevent potential losses of stored carbon and nitrogen following seagrass degradation. , Plain Language Summary In the present study, we explored the abiotic and biotic factors influencing the accumulation patterns of carbon, nitrogen, and carbonate in 15 seagrass ( Posidonia oceanica ) meadows across the South Aegean Sea (Greece). Sediment characteristics (i.e., sediment C org :N and C org :C inorg ratios and P . ocenica contribution to the sediment C org pool) mainly drove the pattern of carbon, nitrogen, and carbonate accumulation, with seagrass traits (i.e., leaf biomass and rhizome elongation) and environmental conditions (i.e., suspended organic matter) contributing to a lesser extent. Overall, seagrass meadows play a crucial role in storing carbon and nitrogen in the studied region, serving as important natural sinks, and thus, protecting these ecosystems is important to prevent the loss of these stored elements. Additionally, understanding the factors that shape the variability in Posidonia oceanica storage capacity is crucial for management and conservation efforts. , Key Points Seagrass ( Posidonia oceanica ) meadows of the South Aegean Sea (Greece) represent important natural sinks for organic carbon, nitrogen, and carbonate. The accumulation pattern of organic carbon, nitrogen, and carbonate in the underlying seagrass sediments was mainly driven by sediment characteristics, followed by seagrass traits and environmental conditions. Fine‐scale assessments are crucial for understanding the local factors shaping the storage capacity of seagrass meadows to support management decisions.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract The variability in stocks and accumulation rates of organic carbon (C org ), nitrogen (N), and carbonate (CaCO 3 ) was studied in fifteen Posidonia oceanica meadows spread throughout the South Aegean Sea (Greece). In addition, the abiotic and biotic drivers determining the pattern of variability in the accumulation rates were assessed by exploring the influence of sediment characteristics, seagrass traits, and environmental settings. The meadows supported on average (±STDEV) 14.6 ± 5.0 kg C org m −2 , 0.47 ± 0.17 kg N m −2 , and 249 ± 210 kg CaCO 3 m −2 in the top meter of their sediments, with mean accumulation rates over the last 500 years of 33.6 ± 23.6 g C org m −2 yr −1 , 1.00 ± 0.62 g N m −2 yr −1 , and 405 ± 336 g CaCO 3 m −2 yr −1 across sites. A redundancy analysis (RDA) explained 70% of the variation in C org , N, and CaCO 3 accumulation rates, with three sediment characteristics (i.e., sediment C org :N and C org :C inorg ratios and P . oceanica contribution to the sediment C org pool) emerging as the primary set of factors shaping the accumulation of matter, followed by seagrass traits (i.e., leaf biomass and rhizome elongation) and environmental variables (i.e., suspended organic matter). The high degree of variability within the region emphasizes the need for fine‐scale assessments to understand the local conditions influencing sequestration. Our findings underscored the critical role of seagrass meadows in carbon and nitrogen sequestration in the region, urging conservation efforts to protect these ecosystems and prevent potential losses of stored carbon and nitrogen following seagrass degradation. , Plain Language Summary In the present study, we explored the abiotic and biotic factors influencing the accumulation patterns of carbon, nitrogen, and carbonate in 15 seagrass ( Posidonia oceanica ) meadows across the South Aegean Sea (Greece). Sediment characteristics (i.e., sediment C org :N and C org :C inorg ratios and P . ocenica contribution to the sediment C org pool) mainly drove the pattern of carbon, nitrogen, and carbonate accumulation, with seagrass traits (i.e., leaf biomass and rhizome elongation) and environmental conditions (i.e., suspended organic matter) contributing to a lesser extent. Overall, seagrass meadows play a crucial role in storing carbon and nitrogen in the studied region, serving as important natural sinks, and thus, protecting these ecosystems is important to prevent the loss of these stored elements. Additionally, understanding the factors that shape the variability in Posidonia oceanica storage capacity is crucial for management and conservation efforts. , Key Points Seagrass ( Posidonia oceanica ) meadows of the South Aegean Sea (Greece) represent important natural sinks for organic carbon, nitrogen, and carbonate. The accumulation pattern of organic carbon, nitrogen, and carbonate in the underlying seagrass sediments was mainly driven by sediment characteristics, followed by seagrass traits and environmental conditions. Fine‐scale assessments are crucial for understanding the local factors shaping the storage capacity of seagrass meadows to support management decisions. |
Rotter, Ana; Varamogianni-Mamatsi, Despoina; Pobirk, Alenka Zvonar; Matjaž, Mirjam Gosenca; Cueto, Mercedes; Díaz-Marrero, Ana R; Jónsdóttir, Rósa; Sveinsdóttir, Kolbrún; Catalá, Teresa S; Romano, Giovanna; Guler, Bahar Aslanbay; Atak, Eylem; Zrimec, Maja Berden; Bosch, Daniel; Deniz, Irem; Gaudêncio, Susana P; Grigalionyte-Bembič, Ernesta; Klun, Katja; Zidar, Luen; Rius, Anna Coll; Baebler, Špela; Bilela, Lada Lukić; Rinkevich, Baruch; Mandalakis, Manolis Marine cosmetics and the blue bioeconomy: From sourcing to success stories Journal Article iScience, 27 (12), pp. 111339, 2024, ISSN: 25890042. @article{rotter_marine_2024, title = {Marine cosmetics and the blue bioeconomy: From sourcing to success stories}, author = {Ana Rotter and Despoina Varamogianni-Mamatsi and Alenka Zvonar Pobirk and Mirjam Gosenca Matjaž and Mercedes Cueto and Ana R Díaz-Marrero and Rósa Jónsdóttir and Kolbrún Sveinsdóttir and Teresa S Catalá and Giovanna Romano and Bahar Aslanbay Guler and Eylem Atak and Maja Berden Zrimec and Daniel Bosch and Irem Deniz and Susana P Gaudêncio and Ernesta Grigalionyte-Bembič and Katja Klun and Luen Zidar and Anna Coll Rius and Špela Baebler and Lada Lukić Bilela and Baruch Rinkevich and Manolis Mandalakis}, url = {https://linkinghub.elsevier.com/retrieve/pii/S2589004224025641 /wp-content/uploads/2024/12/2024-Rotter-iScience-46.pdf}, doi = {10.1016/j.isci.2024.111339}, issn = {25890042}, year = {2024}, date = {2024-12-09}, urldate = {2024-12-10}, journal = {iScience}, volume = {27}, number = {12}, pages = {111339}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Skliros, Dimitrios; Kostakou, Maria; Kokkari, Constantina; Tsertou, Maria Ioanna; Pavloudi, Christina; Zafeiropoulos, Haris; Katharios, Pantelis; Flemetakis, Emmanouil Microorganisms, 12 (11), pp. 2281, 2024, ISSN: 2076-2607. @article{skliros_unveiling_2024, title = {Unveiling Emerging Opportunistic Fish Pathogens in Aquaculture: A Comprehensive Seasonal Study of Microbial Composition in Mediterranean Fish Hatcheries}, author = {Dimitrios Skliros and Maria Kostakou and Constantina Kokkari and Maria Ioanna Tsertou and Christina Pavloudi and Haris Zafeiropoulos and Pantelis Katharios and Emmanouil Flemetakis}, url = {https://www.mdpi.com/2076-2607/12/11/2281 https://imbbc.hcmr.gr/wp-content/uploads/2024/11/2024-Skliros-microorganisms-45.pdf}, doi = {10.3390/microorganisms12112281}, issn = {2076-2607}, year = {2024}, date = {2024-11-12}, urldate = {2024-11-12}, journal = {Microorganisms}, volume = {12}, number = {11}, pages = {2281}, abstract = {The importance of microbial communities in fish hatcheries for fish health and welfare has been recognized, with several studies mapping these communities during healthy rearing conditions and disease outbreaks. In this study, we analyzed the bacteriome of the live feeds, such as microalgae, rotifers, and Artemia, used in fish hatcheries that produce Mediterranean species. Our goal was to provide baseline information about their structure, emphasizing in environmental putative fish pathogenic bacteria. We conducted 16S rRNA amplicon Novaseq sequencing for our analysis, and we inferred 46,745 taxonomically annotated ASVs. Results showed that incoming environmental water plays a significant role in the presence of important taxa that constitute presumptive pathogens. Bio-statistical analyses revealed a relatively stable bacteriome among seasonal samplings for every hatchery but a diverse bacteriome between sampling stations and a distinct core bacteriome for each hatchery. Analysis of putative opportunistic fish pathogenic genera revealed some co-occurrence correlation events and a high average relative abundance of Vibrio, Tenacibaculum, and Photobacterium genera in live feeds, reaching a grand mean average of up to 7.3% for the hatchery of the Hellenic Center of Marine Research (HCMR), 12% for Hatchery A, and 11.5% for Hatchery B. Mapping the bacteriome in live feeds is pivotal for understanding the marine environment and distinct aquaculture practices and can guide improvements in hatchery management, enhancing fish health and sustainability in the Mediterranean region.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The importance of microbial communities in fish hatcheries for fish health and welfare has been recognized, with several studies mapping these communities during healthy rearing conditions and disease outbreaks. In this study, we analyzed the bacteriome of the live feeds, such as microalgae, rotifers, and Artemia, used in fish hatcheries that produce Mediterranean species. Our goal was to provide baseline information about their structure, emphasizing in environmental putative fish pathogenic bacteria. We conducted 16S rRNA amplicon Novaseq sequencing for our analysis, and we inferred 46,745 taxonomically annotated ASVs. Results showed that incoming environmental water plays a significant role in the presence of important taxa that constitute presumptive pathogens. Bio-statistical analyses revealed a relatively stable bacteriome among seasonal samplings for every hatchery but a diverse bacteriome between sampling stations and a distinct core bacteriome for each hatchery. Analysis of putative opportunistic fish pathogenic genera revealed some co-occurrence correlation events and a high average relative abundance of Vibrio, Tenacibaculum, and Photobacterium genera in live feeds, reaching a grand mean average of up to 7.3% for the hatchery of the Hellenic Center of Marine Research (HCMR), 12% for Hatchery A, and 11.5% for Hatchery B. Mapping the bacteriome in live feeds is pivotal for understanding the marine environment and distinct aquaculture practices and can guide improvements in hatchery management, enhancing fish health and sustainability in the Mediterranean region. |
Lanza-Arroyo, Pablo; Sempere-Valverde, Juan; Digenis, Markos; Remón, José Miguel; Moreno, Diego; Barrajón, Agustín; Linde, Antonio De La; Arroyo, Maria Del Carmen; Fernández-Casado, Manuel; Mallofret, Eugenio; Sánchez-Tocino, Luis; Gerovasileiou, Vasilis; Navarro-Barranco, Carlos Baseline for marine cave monitoring strategies in the Alboran Sea using modified Cave Ecosystem-Based Quality Index (CavEBQI) Journal Article Marine Pollution Bulletin, 209 , pp. 117065, 2024, ISSN: 0025326X. @article{lanza-arroyo_baseline_2024, title = {Baseline for marine cave monitoring strategies in the Alboran Sea using modified Cave Ecosystem-Based Quality Index (CavEBQI)}, author = {Pablo Lanza-Arroyo and Juan Sempere-Valverde and Markos Digenis and José Miguel Remón and Diego Moreno and Agustín Barrajón and Antonio De La Linde and Maria Del Carmen Arroyo and Manuel Fernández-Casado and Eugenio Mallofret and Luis Sánchez-Tocino and Vasilis Gerovasileiou and Carlos Navarro-Barranco}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0025326X24010427 /wp-content/uploads/2025/07/2024-Lanza-MPB-52.pdf}, doi = {10.1016/j.marpolbul.2024.117065}, issn = {0025326X}, year = {2024}, date = {2024-10-11}, urldate = {2025-07-08}, journal = {Marine Pollution Bulletin}, volume = {209}, pages = {117065}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Daniso, Enrico; Sarropoulou, Elena; Kaitetzidou, Elisavet; Beraldo, Paola; Tibaldi, Emilio; Cerri, Roberto; Cardinaletti, Gloriana Effect of increasing levels of Hermetia illucens in a fishmeal-free diet at sea bream (Sparus aurata, L.) gastrointestinal level Journal Article Aquaculture Reports, 39 , pp. 102410, 2024, ISSN: 23525134. @article{daniso_effect_2024, title = {Effect of increasing levels of Hermetia illucens in a fishmeal-free diet at sea bream (Sparus aurata, L.) gastrointestinal level}, author = {Enrico Daniso and Elena Sarropoulou and Elisavet Kaitetzidou and Paola Beraldo and Emilio Tibaldi and Roberto Cerri and Gloriana Cardinaletti}, url = {https://linkinghub.elsevier.com/retrieve/pii/S2352513424004988 /wp-content/uploads/2024/10/2024-Daniso-AquaReports-44.pdf}, doi = {10.1016/j.aqrep.2024.102410}, issn = {23525134}, year = {2024}, date = {2024-10-09}, urldate = {2024-10-09}, journal = {Aquaculture Reports}, volume = {39}, pages = {102410}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Kotsiri, Mado; Kleidas, Ioannis; Kogiannou, Dimitra; Vryonides, Katerina; Papadouli, Christina; Golomazou, Eleni; Rigos, George Evaluation of the long-term effects of formaldehyde on the physiology of the Mediterranean mussel, Mytilus galloprovincialis Journal Article Chemosphere, 364 , pp. 143190, 2024, ISSN: 00456535. @article{kotsiri_evaluation_2024, title = {Evaluation of the long-term effects of formaldehyde on the physiology of the Mediterranean mussel, Mytilus galloprovincialis}, author = {Mado Kotsiri and Ioannis Kleidas and Dimitra Kogiannou and Katerina Vryonides and Christina Papadouli and Eleni Golomazou and George Rigos}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0045653524020873 https://imbbc.hcmr.gr/wp-content/uploads/2024/10/2024-Kotsiri-43-pre-print.pdf}, doi = {10.1016/j.chemosphere.2024.143190}, issn = {00456535}, year = {2024}, date = {2024-09-30}, urldate = {2024-10-01}, journal = {Chemosphere}, volume = {364}, pages = {143190}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Reverté, Jaume; Rambla-Alegre, Maria; Sanchez-Henao, Andres; Mandalakis, Manolis; Peristeraki, Panagiota; Molgó, Jordi; Diogène, Jorge; Sureda, Francesc X; Campàs, Mònica Toxicity Equivalency Factors for Tetrodotoxin Analogues Determined with Automated Patch Clamp on Voltage-Gated Sodium Channels in Neuro-2a Cells Journal Article Journal of Agricultural and Food Chemistry, 72 (32), pp. 18192–18200, 2024, ISSN: 0021-8561, 1520-5118. @article{reverte_toxicity_2024, title = {Toxicity Equivalency Factors for Tetrodotoxin Analogues Determined with Automated Patch Clamp on Voltage-Gated Sodium Channels in Neuro-2a Cells}, author = {Jaume Reverté and Maria Rambla-Alegre and Andres Sanchez-Henao and Manolis Mandalakis and Panagiota Peristeraki and Jordi Molgó and Jorge Diogène and Francesc X Sureda and Mònica Campàs}, url = {https://pubs.acs.org/doi/10.1021/acs.jafc.4c04321 https://imbbc.hcmr.gr/wp-content/uploads/2024/09/2024-Reverte-JAFC-42-pre-print.pdf}, doi = {10.1021/acs.jafc.4c04321}, issn = {0021-8561, 1520-5118}, year = {2024}, date = {2024-09-26}, urldate = {2024-09-26}, journal = {Journal of Agricultural and Food Chemistry}, volume = {72}, number = {32}, pages = {18192--18200}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Skouradakis, Grigorios; Vernadou, Emmanouela; Koulouri, Panayota; Dailianis, Thanos Mass mortality of the invasive echinoid Diadema setosum (Leske, 1778) in Crete, East Mediterranean Sea Journal Article Mediterranean Marine Science, 25 (2), pp. 480–483, 2024, ISSN: 1791-6763, 1108-393X. @article{skouradakis_mass_2024, title = {Mass mortality of the invasive echinoid Diadema setosum (Leske, 1778) in Crete, East Mediterranean Sea}, author = {Grigorios Skouradakis and Emmanouela Vernadou and Panayota Koulouri and Thanos Dailianis}, url = {https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/36447 https://imbbc.hcmr.gr/wp-content/uploads/2024/09/2024-Skouradakis-MMS-41.pdf}, doi = {10.12681/mms.36447}, issn = {1791-6763, 1108-393X}, year = {2024}, date = {2024-09-23}, urldate = {2024-09-23}, journal = {Mediterranean Marine Science}, volume = {25}, number = {2}, pages = {480--483}, abstract = {Diadema setosum is an echinoid of Indo-Pacific origin that invaded the Mediterranean Sea in 2006. It is an ecosystem engineer with an important ecological function in its native range, but it can have a detrimental effect on Mediterranean reefs. Recently in 2022 a mass mortality event (MME) affecting this species was recorded in the east Aegean Sea in the eastern part of the Mediterranean basin. We are reporting herein a westward progression of the MME in 2023 affecting established populations in various locations around the island of Crete.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Diadema setosum is an echinoid of Indo-Pacific origin that invaded the Mediterranean Sea in 2006. It is an ecosystem engineer with an important ecological function in its native range, but it can have a detrimental effect on Mediterranean reefs. Recently in 2022 a mass mortality event (MME) affecting this species was recorded in the east Aegean Sea in the eastern part of the Mediterranean basin. We are reporting herein a westward progression of the MME in 2023 affecting established populations in various locations around the island of Crete. |
Lavecchia, Anna; Virgilio, Caterina De; Mansi, Luigi; Manzari, Caterina; Mylonas, Constantinos C; Picardi, Ernesto; Pousis, Chrysovalentinos; Cox, Sharon N; Ventriglia, Gianluca; Zupa, Rosa; Pesole, Graziano; Corriero, Aldo Comparison of ovarian mRNA expression levels in wild and hatchery-produced greater amberjack Seriola dumerili Journal Article Scientific Reports, 14 (1), pp. 18034, 2024, ISSN: 2045-2322. @article{lavecchia_comparison_2024, title = {Comparison of ovarian mRNA expression levels in wild and hatchery-produced greater amberjack Seriola dumerili}, author = {Anna Lavecchia and Caterina De Virgilio and Luigi Mansi and Caterina Manzari and Constantinos C Mylonas and Ernesto Picardi and Chrysovalentinos Pousis and Sharon N Cox and Gianluca Ventriglia and Rosa Zupa and Graziano Pesole and Aldo Corriero}, url = {https://www.nature.com/articles/s41598-024-69091-2 https://imbbc.hcmr.gr/wp-content/uploads/2024/09/2024-Lavecchia-SciRep-40.pdf}, doi = {10.1038/s41598-024-69091-2}, issn = {2045-2322}, year = {2024}, date = {2024-09-16}, urldate = {2024-09-16}, journal = {Scientific Reports}, volume = {14}, number = {1}, pages = {18034}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
A, Collective Article New records of introduced species in the Mediterranean (August 2024) Journal Article Mediterranean Marine Science, 2024, ISSN: 1791-6763, 1108-393X. @article{A2025, title = {New records of introduced species in the Mediterranean (August 2024)}, author = {Collective Article A}, url = {https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/34474 /wp-content/uploads/2025/06/2024-Christidis-MMS-51.pdf }, doi = {10.12681/mms.34474}, issn = {1791-6763, 1108-393X}, year = {2024}, date = {2024-08-30}, urldate = {2025-06-04}, journal = {Mediterranean Marine Science}, abstract = {New records of introduced species in the Mediterranean (August 2024) }, keywords = {}, pubstate = {published}, tppubtype = {article} } New records of introduced species in the Mediterranean (August 2024) |