2024 |
García-Escudero, Catalina A; Tsigenopoulos, Costas S; Manousaki, Tereza; Tsakogiannis, Alexandros; Marbà, Núria; Vizzini, Salvatrice; Duarte, Carlos M; Apostolaki, Eugenia T Population genomics unveils the century-old invasion of the Seagrass Halophila stipulacea in the Mediterranean Sea Journal Article Marine Biology, 171 (2), pp. 40, 2024, ISSN: 0025-3162, 1432-1793. @article{garcia-escudero_population_2024, title = {Population genomics unveils the century-old invasion of the Seagrass Halophila stipulacea in the Mediterranean Sea}, author = {Catalina A García-Escudero and Costas S Tsigenopoulos and Tereza Manousaki and Alexandros Tsakogiannis and Núria Marbà and Salvatrice Vizzini and Carlos M Duarte and Eugenia T Apostolaki}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2024/01/2024-Garcia-Escudero-2.pdf https://link.springer.com/10.1007/s00227-023-04361-7}, doi = {10.1007/s00227-023-04361-7}, issn = {0025-3162, 1432-1793}, year = {2024}, date = {2024-01-11}, urldate = {2024-01-19}, journal = {Marine Biology}, volume = {171}, number = {2}, pages = {40}, abstract = {Abstract The tropical seagrass Halophila stipulacea invaded the Eastern Mediterranean Sea in the late nineteenth century and progressively spread throughout the basin ever since. Its spread is expected to continue north-westward as the Mediterranean Sea becomes warmer, potentially changing the seagrass biogeography of the basin. Given the power of genomics to assess invasion dynamics in non-model species, we report the first ddRAD-seq study of H. stipulacea and small-scale population genomic analysis addressing its century-old Mediterranean invasion. Based on 868 SNPs and 35 genotyped native (Red Sea) and exotic (from Cyprus, Greece, and Italy) samples, results suggest that genetic structure was high, especially between major geographic discontinuities, and that exotic populations maintain comparably lower genetic diversity than native populations, despite 130 years of invasion. The evidence of high heterozygosity excess, coupled with previously reported male-dominated and rare flowering records in the exotic range, suggests that clonal propagation likely played a pivotal role in the successful colonization and spread of H. stipulacea in the Mediterranean. This shift in reproductive strategy, particularly evident in the Italian populations located closest to the western boundary and representing more recent establishments, underscores the importance of this cost-effective mode of reproduction, especially during the initial stages of invasion, raising questions about the species future expansion trajectory. Our findings serve as a catalyst for future research into the species’ invasion dynamics, including deciphering the intricate roles of acclimatization and rapid adaptation, important for a comprehensive assessment of invasion risks and improving management strategies aimed at conserving seagrass ecosystems.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract The tropical seagrass Halophila stipulacea invaded the Eastern Mediterranean Sea in the late nineteenth century and progressively spread throughout the basin ever since. Its spread is expected to continue north-westward as the Mediterranean Sea becomes warmer, potentially changing the seagrass biogeography of the basin. Given the power of genomics to assess invasion dynamics in non-model species, we report the first ddRAD-seq study of H. stipulacea and small-scale population genomic analysis addressing its century-old Mediterranean invasion. Based on 868 SNPs and 35 genotyped native (Red Sea) and exotic (from Cyprus, Greece, and Italy) samples, results suggest that genetic structure was high, especially between major geographic discontinuities, and that exotic populations maintain comparably lower genetic diversity than native populations, despite 130 years of invasion. The evidence of high heterozygosity excess, coupled with previously reported male-dominated and rare flowering records in the exotic range, suggests that clonal propagation likely played a pivotal role in the successful colonization and spread of H. stipulacea in the Mediterranean. This shift in reproductive strategy, particularly evident in the Italian populations located closest to the western boundary and representing more recent establishments, underscores the importance of this cost-effective mode of reproduction, especially during the initial stages of invasion, raising questions about the species future expansion trajectory. Our findings serve as a catalyst for future research into the species’ invasion dynamics, including deciphering the intricate roles of acclimatization and rapid adaptation, important for a comprehensive assessment of invasion risks and improving management strategies aimed at conserving seagrass ecosystems. |
2023 |
Papadogiannis, Vasileios; Manousaki, Tereza; Nousias, Orestis; Tsakogiannis, Alexandros; Kristoffersen, Jon B; Mylonas, Constantinos C; Batargias, Costas; Chatziplis, Dimitrios; Tsigenopoulos, Costas S Chromosome genome assembly for the meagre, Argyrosomus regius, reveals species adaptations and sciaenid sex-related locus evolution Journal Article Frontiers in Genetics, 13 , pp. 1081760, 2023, ISSN: 1664-8021. @article{papadogiannis_chromosome_2023b, title = {Chromosome genome assembly for the meagre, Argyrosomus regius, reveals species adaptations and sciaenid sex-related locus evolution}, author = {Vasileios Papadogiannis and Tereza Manousaki and Orestis Nousias and Alexandros Tsakogiannis and Jon B Kristoffersen and Constantinos C Mylonas and Costas Batargias and Dimitrios Chatziplis and Costas S Tsigenopoulos}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/07/2023-Papadogiannis-FrontGen-34.pdf https://www.frontiersin.org/articles/10.3389/fgene.2022.1081760/full}, doi = {10.3389/fgene.2022.1081760}, issn = {1664-8021}, year = {2023}, date = {2023-07-03}, urldate = {2023-07-03}, journal = {Frontiers in Genetics}, volume = {13}, pages = {1081760}, abstract = {The meagre, Argyrosomus regius , has recently become a species of increasing economic interest for the Mediterranean aquaculture and there is ongoing work to boost production efficiency through selective breeding. Access to the complete genomic sequence will provide an essential resource for studying quantitative trait-associated loci and exploring the genetic diversity of different wild populations and aquaculture stocks in more detail. Here, we present the first complete genome for A. regius , produced through a combination of long and short read technologies and an efficient in-house developed pipeline for assembly and polishing. Scaffolding using previous linkage map data allowed us to reconstruct a chromosome level assembly with high completeness, complemented with gene annotation and repeat masking. The 696 Mb long assembly has an N50 = 27.87 Mb and an L50 = 12, with 92.85% of its length placed in 24 chromosomes. We use this new resource to study the evolution of the meagre genome and other Sciaenids, via a comparative analysis of 25 high-quality teleost genomes. Combining a rigorous investigation of gene duplications with base-wise conservation analysis, we identify candidate loci related to immune, fat metabolism and growth adaptations in the meagre. Following phylogenomic reconstruction, we show highly conserved synteny within Sciaenidae. In contrast, we report rapidly evolving syntenic rearrangements and gene copy changes in the sex-related dmrt1 neighbourhood in meagre and other members of the family. These novel genomic datasets and findings will add important new tools for aquaculture studies and greatly facilitate husbandry and breeding work in the species.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The meagre, Argyrosomus regius , has recently become a species of increasing economic interest for the Mediterranean aquaculture and there is ongoing work to boost production efficiency through selective breeding. Access to the complete genomic sequence will provide an essential resource for studying quantitative trait-associated loci and exploring the genetic diversity of different wild populations and aquaculture stocks in more detail. Here, we present the first complete genome for A. regius , produced through a combination of long and short read technologies and an efficient in-house developed pipeline for assembly and polishing. Scaffolding using previous linkage map data allowed us to reconstruct a chromosome level assembly with high completeness, complemented with gene annotation and repeat masking. The 696 Mb long assembly has an N50 = 27.87 Mb and an L50 = 12, with 92.85% of its length placed in 24 chromosomes. We use this new resource to study the evolution of the meagre genome and other Sciaenids, via a comparative analysis of 25 high-quality teleost genomes. Combining a rigorous investigation of gene duplications with base-wise conservation analysis, we identify candidate loci related to immune, fat metabolism and growth adaptations in the meagre. Following phylogenomic reconstruction, we show highly conserved synteny within Sciaenidae. In contrast, we report rapidly evolving syntenic rearrangements and gene copy changes in the sex-related dmrt1 neighbourhood in meagre and other members of the family. These novel genomic datasets and findings will add important new tools for aquaculture studies and greatly facilitate husbandry and breeding work in the species. |
Papadogiannis, Vasileios; Manousaki, Tereza; Nousias, Orestis; Tsakogiannis, Alexandros; Kristoffersen, Jon B; Mylonas, Constantinos C; Batargias, Costas; Chatziplis, Dimitrios; Tsigenopoulos, Costas S Chromosome genome assembly for the meagre, Argyrosomus regius, reveals species adaptations and sciaenid sex-related locus evolution Journal Article Frontiers in Genetics, 13 , pp. 1081760, 2023, ISSN: 1664-8021. @article{papadogiannis_chromosome_2023, title = {Chromosome genome assembly for the meagre, Argyrosomus regius, reveals species adaptations and sciaenid sex-related locus evolution}, author = {Vasileios Papadogiannis and Tereza Manousaki and Orestis Nousias and Alexandros Tsakogiannis and Jon B Kristoffersen and Constantinos C Mylonas and Costas Batargias and Dimitrios Chatziplis and Costas S Tsigenopoulos}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2023/01/2023-Papadogiannis-FinGenetics-4.pdf https://www.frontiersin.org/articles/10.3389/fgene.2022.1081760/full}, doi = {10.3389/fgene.2022.1081760}, issn = {1664-8021}, year = {2023}, date = {2023-01-10}, urldate = {2023-01-11}, journal = {Frontiers in Genetics}, volume = {13}, pages = {1081760}, abstract = {The meagre, Argyrosomus regius , has recently become a species of increasing economic interest for the Mediterranean aquaculture and there is ongoing work to boost production efficiency through selective breeding. Access to the complete genomic sequence will provide an essential resource for studying quantitative trait-associated loci and exploring the genetic diversity of different wild populations and aquaculture stocks in more detail. Here, we present the first complete genome for A. regius , produced through a combination of long and short read technologies and an efficient in-house developed pipeline for assembly and polishing. Scaffolding using previous linkage map data allowed us to reconstruct a chromosome level assembly with high completeness, complemented with gene annotation and repeat masking. The 696 Mb long assembly has an N50 = 27.87 Mb and an L50 = 12, with 92.85% of its length placed in 24 chromosomes. We use this new resource to study the evolution of the meagre genome and other Sciaenids, via a comparative analysis of 25 high-quality teleost genomes. Combining a rigorous investigation of gene duplications with base-wise conservation analysis, we identify candidate loci related to immune, fat metabolism and growth adaptations in the meagre. Following phylogenomic reconstruction, we show highly conserved synteny within Sciaenidae. In contrast, we report rapidly evolving syntenic rearrangements and gene copy changes in the sex-related dmrt1 neighbourhood in meagre and other members of the family. These novel genomic datasets and findings will add important new tools for aquaculture studies and greatly facilitate husbandry and breeding work in the species.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The meagre, Argyrosomus regius , has recently become a species of increasing economic interest for the Mediterranean aquaculture and there is ongoing work to boost production efficiency through selective breeding. Access to the complete genomic sequence will provide an essential resource for studying quantitative trait-associated loci and exploring the genetic diversity of different wild populations and aquaculture stocks in more detail. Here, we present the first complete genome for A. regius , produced through a combination of long and short read technologies and an efficient in-house developed pipeline for assembly and polishing. Scaffolding using previous linkage map data allowed us to reconstruct a chromosome level assembly with high completeness, complemented with gene annotation and repeat masking. The 696 Mb long assembly has an N50 = 27.87 Mb and an L50 = 12, with 92.85% of its length placed in 24 chromosomes. We use this new resource to study the evolution of the meagre genome and other Sciaenids, via a comparative analysis of 25 high-quality teleost genomes. Combining a rigorous investigation of gene duplications with base-wise conservation analysis, we identify candidate loci related to immune, fat metabolism and growth adaptations in the meagre. Following phylogenomic reconstruction, we show highly conserved synteny within Sciaenidae. In contrast, we report rapidly evolving syntenic rearrangements and gene copy changes in the sex-related dmrt1 neighbourhood in meagre and other members of the family. These novel genomic datasets and findings will add important new tools for aquaculture studies and greatly facilitate husbandry and breeding work in the species. |
2022 |
Ramos-Júdez, Sandra; Danis, Theodoros; Angelova, Nelina; Tsakogiannis, Alexandros; Giménez, Ignacio; Tsigenopoulos, Costas S; Duncan, Neil; Manousaki, Tereza Transcriptome analysis of flathead grey mullet (Mugil cephalus) ovarian development induced by recombinant gonadotropin hormones Journal Article Frontiers in Physiology, 13 , pp. 1033445, 2022, ISSN: 1664-042X. @article{ramos-judez_transcriptome_2022, title = {Transcriptome analysis of flathead grey mullet (Mugil cephalus) ovarian development induced by recombinant gonadotropin hormones}, author = {Sandra Ramos-Júdez and Theodoros Danis and Nelina Angelova and Alexandros Tsakogiannis and Ignacio Giménez and Costas S Tsigenopoulos and Neil Duncan and Tereza Manousaki}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/11/2022-Ramos-Judez-fphys-75-1.pdf https://www.frontiersin.org/articles/10.3389/fphys.2022.1033445/full}, doi = {10.3389/fphys.2022.1033445}, issn = {1664-042X}, year = {2022}, date = {2022-11-14}, urldate = {2022-11-14}, journal = {Frontiers in Physiology}, volume = {13}, pages = {1033445}, abstract = {Background: Treatment with recombinant gonadotropin hormones (rGths), follicle-stimulating hormone (rFsh) and luteinizing hormone (rLh), was shown to induce and complete vitellogenesis to finally obtain viable eggs and larvae in the flathead grey mullet ( Mugil cephalus ), a teleost arrested at early stages of gametogenesis in intensive captivity conditions. This study aimed to investigate the transcriptomic changes that occur in the ovary of females during the rGths-induced vitellogenesis. Methods: Ovarian samples were collected through biopsies from the same five females at four stages of ovarian development. RNASeq libraries were constructed for all stages studied, sequenced on an Illumina HiSeq4000, and a de novo transcriptome was constructed. Differentially expressed genes (DEGs) were identified between stages and the functional properties of DEGs were characterized by comparison with the gene ontology and Kyoto Encyclopedia. An enrichment analysis of molecular pathways was performed. Results: The de novo transcriptome comprised 287,089 transcripts after filtering. As vitellogenesis progressed, more genes were significantly upregulated than downregulated. The rFsh application induced ovarian development from previtellogenesis to early-to-mid-vitellogenesis with associated pathways enriched from upregulated DEGs related to ovarian steroidogenesis and reproductive development , cholesterol metabolism, ovarian growth and differentiation, lipid accumulation, and cell-to-cell adhesion pathways. The application of rFsh and rLh at early-to-mid-vitellogenesis induced the growth of oocytes to late-vitellogenesis and, with it, the enrichment of pathways from upregulated DEGs related to the production of energy, such as the lysosomes activity . The application of rLh at late-vitellogenesis induced the completion of vitellogenesis with the enrichment of pathways linked with the switch from vitellogenesis to oocyte maturation. Conclusion: The DEGs and enriched molecular pathways described during the induced vitellogenesis of flathead grey mullet with rGths were typical of natural oogenesis reported for other fish species. Present results add new knowledge to the rGths action to further raise the possibility of using rGths in species that present similar reproductive disorders in aquaculture, the aquarium industry as well as the conservation of endangered species.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Background: Treatment with recombinant gonadotropin hormones (rGths), follicle-stimulating hormone (rFsh) and luteinizing hormone (rLh), was shown to induce and complete vitellogenesis to finally obtain viable eggs and larvae in the flathead grey mullet ( Mugil cephalus ), a teleost arrested at early stages of gametogenesis in intensive captivity conditions. This study aimed to investigate the transcriptomic changes that occur in the ovary of females during the rGths-induced vitellogenesis. Methods: Ovarian samples were collected through biopsies from the same five females at four stages of ovarian development. RNASeq libraries were constructed for all stages studied, sequenced on an Illumina HiSeq4000, and a de novo transcriptome was constructed. Differentially expressed genes (DEGs) were identified between stages and the functional properties of DEGs were characterized by comparison with the gene ontology and Kyoto Encyclopedia. An enrichment analysis of molecular pathways was performed. Results: The de novo transcriptome comprised 287,089 transcripts after filtering. As vitellogenesis progressed, more genes were significantly upregulated than downregulated. The rFsh application induced ovarian development from previtellogenesis to early-to-mid-vitellogenesis with associated pathways enriched from upregulated DEGs related to ovarian steroidogenesis and reproductive development , cholesterol metabolism, ovarian growth and differentiation, lipid accumulation, and cell-to-cell adhesion pathways. The application of rFsh and rLh at early-to-mid-vitellogenesis induced the growth of oocytes to late-vitellogenesis and, with it, the enrichment of pathways from upregulated DEGs related to the production of energy, such as the lysosomes activity . The application of rLh at late-vitellogenesis induced the completion of vitellogenesis with the enrichment of pathways linked with the switch from vitellogenesis to oocyte maturation. Conclusion: The DEGs and enriched molecular pathways described during the induced vitellogenesis of flathead grey mullet with rGths were typical of natural oogenesis reported for other fish species. Present results add new knowledge to the rGths action to further raise the possibility of using rGths in species that present similar reproductive disorders in aquaculture, the aquarium industry as well as the conservation of endangered species. |
García-Escudero, Catalina A; Tsigenopoulos, Costas S; Gerakaris, Vasilis; Tsakogiannis, Alexandros; Apostolaki, Eugenia T ITS DNA Barcoding Reveals That Halophila stipulacea Still Remains the Only Non-Indigenous Seagrass of the Mediterranean Sea Journal Article Diversity, 14 (2), pp. 76, 2022, ISSN: 1424-2818. @article{garcia-escudero_its_2022, title = {ITS DNA Barcoding Reveals That Halophila stipulacea Still Remains the Only Non-Indigenous Seagrass of the Mediterranean Sea}, author = {Catalina A García-Escudero and Costas S Tsigenopoulos and Vasilis Gerakaris and Alexandros Tsakogiannis and Eugenia T Apostolaki}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/03/2022-Garcia-Escudero-DIVERSITY-8.pdf https://www.mdpi.com/1424-2818/14/2/76}, doi = {10.3390/d14020076}, issn = {1424-2818}, year = {2022}, date = {2022-01-01}, urldate = {2022-03-11}, journal = {Diversity}, volume = {14}, number = {2}, pages = {76}, abstract = {Non-indigenous species (NIS) are one of the major threats to the native marine ecosystems of the Mediterranean Sea. Halophila stipulacea was the only exotic seagrass of the Mediterranean until 2018, when small patches of a species morphologically identified as Halophila decipiens were reported in Salamina Island, Greece. Given the absence of reproductive structures during the identification and the taxonomic ambiguities known to lead to misidentifications on this genus, we reassessed the identity of this new exotic record using DNA barcoding (rbcL, matK and ITS) and the recently published taxonomic key. Despite their morphologic similarity to H. decipiens based on the new taxonomic key, the specimens showed no nucleotide differences with H. stipulacea specimens (Crete) for the three barcodes and clustered together on the ITS phylogenetic tree. Considering the high species resolution of the ITS region and the common morphological variability within the genus, the unequivocal genetic result suggests that the Halophila population found in Salamina Island most likely corresponds to a morphologically variant H. stipulacea. Our results highlight the importance of applying an integrated taxonomic approach (morphological and molecular) to taxonomically complex genera such as Halophila, in order to avoid overlooking or misreporting species range shifts, which is essential for monitoring NIS introductions.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Non-indigenous species (NIS) are one of the major threats to the native marine ecosystems of the Mediterranean Sea. Halophila stipulacea was the only exotic seagrass of the Mediterranean until 2018, when small patches of a species morphologically identified as Halophila decipiens were reported in Salamina Island, Greece. Given the absence of reproductive structures during the identification and the taxonomic ambiguities known to lead to misidentifications on this genus, we reassessed the identity of this new exotic record using DNA barcoding (rbcL, matK and ITS) and the recently published taxonomic key. Despite their morphologic similarity to H. decipiens based on the new taxonomic key, the specimens showed no nucleotide differences with H. stipulacea specimens (Crete) for the three barcodes and clustered together on the ITS phylogenetic tree. Considering the high species resolution of the ITS region and the common morphological variability within the genus, the unequivocal genetic result suggests that the Halophila population found in Salamina Island most likely corresponds to a morphologically variant H. stipulacea. Our results highlight the importance of applying an integrated taxonomic approach (morphological and molecular) to taxonomically complex genera such as Halophila, in order to avoid overlooking or misreporting species range shifts, which is essential for monitoring NIS introductions. |
Nousias, O; Oikonomou, S; Manousaki, T; Papadogiannis, V; Angelova, N; Tsaparis, D; Tsakogiannis, A; Duncan, N; Estevez, A; Tzokas, K; Pavlidis, M; Chatziplis, D; Tsigenopoulos, C S Scientific Reports, 12 (1), pp. 5301, 2022, ISSN: 2045-2322. @article{nousias_linkage_2022, title = {Linkage mapping, comparative genome analysis, and QTL detection for growth in a non-model teleost, the meagre Argyrosomus regius, using ddRAD sequencing}, author = {O Nousias and S Oikonomou and T Manousaki and V Papadogiannis and N Angelova and D Tsaparis and A Tsakogiannis and N Duncan and A Estevez and K Tzokas and M Pavlidis and D Chatziplis and C S Tsigenopoulos}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/05/2022-Nousias-Sci-Report-27.pdf https://www.nature.com/articles/s41598-022-09289-4}, doi = {10.1038/s41598-022-09289-4}, issn = {2045-2322}, year = {2022}, date = {2022-01-01}, urldate = {2022-05-09}, journal = {Scientific Reports}, volume = {12}, number = {1}, pages = {5301}, abstract = {Abstract Meagre ( Argyrosomus regius ), is a benthopelagic species rapidly emerging in aquaculture, due to its low food to biomass conversion rate, good fillet yield and ease of production. Tracing a species genomic background along with describing the genetic basis of important traits can greatly influence both conservation strategies and production perspectives. In this study, we employed ddRAD sequencing of 266 fish from six F1 meagre families, to construct a high-density genetic map comprising 4529 polymorphic SNP markers. The QTL mapping analysis provided a genomic appreciation for the weight trait identifying a statistically significant QTL on linkage group 15 (LG15). The comparative genomics analysis with six teleost species revealed an evolutionarily conserved karyotype structure. The synteny observed, verified the already well-known fusion events of the three-spine stickleback genome, reinforced the evidence of reduced evolutionary distance of Sciaenids with the Sparidae family, reflected the evolutionary proximity with Dicentrarchus labrax , traced several putative chromosomal rearrangements and a prominent putative fusion event in meagre’s LG17. This study presents novel elements concerning the genome evolutionary history of a non-model teleost species recently adopted in aquaculture, starts to unravel the genetic basis of the species growth-related traits, and provides a high-density genetic map as a tool that can help to further establish meagre as a valuable resource for research and production.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Meagre ( Argyrosomus regius ), is a benthopelagic species rapidly emerging in aquaculture, due to its low food to biomass conversion rate, good fillet yield and ease of production. Tracing a species genomic background along with describing the genetic basis of important traits can greatly influence both conservation strategies and production perspectives. In this study, we employed ddRAD sequencing of 266 fish from six F1 meagre families, to construct a high-density genetic map comprising 4529 polymorphic SNP markers. The QTL mapping analysis provided a genomic appreciation for the weight trait identifying a statistically significant QTL on linkage group 15 (LG15). The comparative genomics analysis with six teleost species revealed an evolutionarily conserved karyotype structure. The synteny observed, verified the already well-known fusion events of the three-spine stickleback genome, reinforced the evidence of reduced evolutionary distance of Sciaenids with the Sparidae family, reflected the evolutionary proximity with Dicentrarchus labrax , traced several putative chromosomal rearrangements and a prominent putative fusion event in meagre’s LG17. This study presents novel elements concerning the genome evolutionary history of a non-model teleost species recently adopted in aquaculture, starts to unravel the genetic basis of the species growth-related traits, and provides a high-density genetic map as a tool that can help to further establish meagre as a valuable resource for research and production. |
2021 |
Oikonomou, Stavroula; Tsakogiannis, Alexandros; Kriaridou, Christina; Danis, Theodoros; Manousaki, Tereza; Chatziplis, Dimitris; Papandroulakis, Nikos; Mylonas, Constantinos C; Triantafyllidis, Alexandros; Tsigenopoulos, Costas S 21 , pp. 100855, 2021, ISSN: 23525134. @article{oikonomou_first_2021, title = {First linkage maps and a pilot QTL analysis for early growth performance in common dentex (Dentex dentex) and sharpsnout seabream (Diplodus puntazzo)}, author = {Stavroula Oikonomou and Alexandros Tsakogiannis and Christina Kriaridou and Theodoros Danis and Tereza Manousaki and Dimitris Chatziplis and Nikos Papandroulakis and Constantinos C Mylonas and Alexandros Triantafyllidis and Costas S Tsigenopoulos}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/09/2021-Oikonomou-AquaReports-68.pdf https://linkinghub.elsevier.com/retrieve/pii/S2352513421002714}, doi = {10.1016/j.aqrep.2021.100855}, issn = {23525134}, year = {2021}, date = {2021-09-01}, urldate = {2021-09-22}, volume = {21}, pages = {100855}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Peñaloza, C; Manousaki, T; Franch, R; Tsakogiannis, A; Sonesson, A K; Aslam, M L; Allal, F; Bargelloni, L; Houston, R D; Tsigenopoulos, C S Development and testing of a combined species SNP array for the European seabass (Dicentrarchus labrax) and gilthead seabream (Sparus aurata) Journal Article Genomics, 113 (4), pp. 2096–2107, 2021, ISSN: 08887543. @article{penaloza_development_2021, title = {Development and testing of a combined species SNP array for the European seabass (Dicentrarchus labrax) and gilthead seabream (Sparus aurata)}, author = {C Peñaloza and T Manousaki and R Franch and A Tsakogiannis and A K Sonesson and M L Aslam and F Allal and L Bargelloni and R D Houston and C S Tsigenopoulos}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/06/2021-Penaloza-Genomics-39.pdf https://linkinghub.elsevier.com/retrieve/pii/S0888754321001713}, doi = {10.1016/j.ygeno.2021.04.038}, issn = {08887543}, year = {2021}, date = {2021-07-01}, urldate = {2021-06-24}, journal = {Genomics}, volume = {113}, number = {4}, pages = {2096--2107}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Mladineo, Ivona; Hrabar, Jerko; Trumbić, Željka; Manousaki, Tereza; Tsakogiannis, Alexandros; Taggart, John B; Tsigenopoulos, Costas S Pathogens, 10 (2), pp. 100, 2021, ISSN: 2076-0817. @article{mladineo_community_2021, title = {Community Parameters and Genome-Wide RAD-Seq Loci of Ceratothoa oestroides Imply Its Transfer between Farmed European Sea Bass and Wild Farm-Aggregating Fish}, author = {Ivona Mladineo and Jerko Hrabar and Željka Trumbić and Tereza Manousaki and Alexandros Tsakogiannis and John B Taggart and Costas S Tsigenopoulos}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2021/02/2020-Mladineo-Pathogens-7.pdf https://www.mdpi.com/2076-0817/10/2/100}, doi = {10.3390/pathogens10020100}, issn = {2076-0817}, year = {2021}, date = {2021-01-01}, urldate = {2021-02-02}, journal = {Pathogens}, volume = {10}, number = {2}, pages = {100}, abstract = {Wild fish assemblages that aggregate within commercial marine aquaculture sites for feeding and shelter have been considered as a primary source of pathogenic parasites vectored to farmed fish maintained in net pens at an elevated density. In order to evaluate whether Ceratothoa oestroides (Isopoda, Cymothoidae), a generalist and pestilent isopod that is frequently found in Adriatic and Greek stocks of farmed European sea bass (Dicentrarchus labrax), transfers between wild and farmed fish, a RAD-Seq (restriction-site-associated DNA sequencing)-mediated genetic screening approach was employed. The double-digest RAD-Seq of 310 C. oestroides specimens collected from farmed European sea bass (138) and different wild farm-aggregating fish (172) identified 313 robust SNPs that evidenced a close genetic relatedness between the “wild” and “farmed” genotypes. ddRAD-Seq proved to be an effective method for detecting the discrete genetic structuring of C. oestroides and genotype intermixing between two populations. The parasite prevalence in the farmed sea bass was 1.02%, with a mean intensity of 2.0 and mean abundance of 0.02, while in the wild fish, the prevalence was 8.1%; the mean intensity, 1.81; and the mean abundance, 0.15. Such differences are likely a consequence of human interventions during the farmed fish’s rearing cycle that, nevertheless, did not affect the transfer of C. oestroides.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Wild fish assemblages that aggregate within commercial marine aquaculture sites for feeding and shelter have been considered as a primary source of pathogenic parasites vectored to farmed fish maintained in net pens at an elevated density. In order to evaluate whether Ceratothoa oestroides (Isopoda, Cymothoidae), a generalist and pestilent isopod that is frequently found in Adriatic and Greek stocks of farmed European sea bass (Dicentrarchus labrax), transfers between wild and farmed fish, a RAD-Seq (restriction-site-associated DNA sequencing)-mediated genetic screening approach was employed. The double-digest RAD-Seq of 310 C. oestroides specimens collected from farmed European sea bass (138) and different wild farm-aggregating fish (172) identified 313 robust SNPs that evidenced a close genetic relatedness between the “wild” and “farmed” genotypes. ddRAD-Seq proved to be an effective method for detecting the discrete genetic structuring of C. oestroides and genotype intermixing between two populations. The parasite prevalence in the farmed sea bass was 1.02%, with a mean intensity of 2.0 and mean abundance of 0.02, while in the wild fish, the prevalence was 8.1%; the mean intensity, 1.81; and the mean abundance, 0.15. Such differences are likely a consequence of human interventions during the farmed fish’s rearing cycle that, nevertheless, did not affect the transfer of C. oestroides. |
Danis, Theodoros; Papadogiannis, Vasileios; Tsakogiannis, Alexandros; Kristoffersen, Jon B; Golani, Daniel; Tsaparis, Dimitris; Sterioti, Aspasia; Kasapidis, Panagiotis; Kotoulas, Georgios; Magoulas, Antonios; Tsigenopoulos, Costas S; Manousaki, Tereza Genome Analysis of Lagocephalus sceleratus: Unraveling the Genomic Landscape of a Successful Invader Journal Article Frontiers in Genetics, 12 , pp. 2481, 2021, ISSN: 1664-8021. @article{danis_genome_2021, title = {Genome Analysis of Lagocephalus sceleratus: Unraveling the Genomic Landscape of a Successful Invader}, author = {Theodoros Danis and Vasileios Papadogiannis and Alexandros Tsakogiannis and Jon B Kristoffersen and Daniel Golani and Dimitris Tsaparis and Aspasia Sterioti and Panagiotis Kasapidis and Georgios Kotoulas and Antonios Magoulas and Costas S Tsigenopoulos and Tereza Manousaki}, url = {https://imbbc.hcmr.gr/wp-content/uploads/2022/01/2021-Danis-FrontGen-84.pdf https://www.frontiersin.org/article/10.3389/fgene.2021.790850}, doi = {10.3389/fgene.2021.790850}, issn = {1664-8021}, year = {2021}, date = {2021-01-01}, urldate = {2022-01-12}, journal = {Frontiers in Genetics}, volume = {12}, pages = {2481}, abstract = {The Tetraodontidae family encompasses several species which attract scientific interest in terms of their ecology and evolution. The silver-cheeked toadfish (Lagocephalus sceleratus) is a well-known “invasive sprinter” that has invaded and spread, in less than a decade, throughout the Eastern and part of the Western Mediterranean Sea from the Red Sea through the Suez Canal. In this study, we built and analysed the first near-chromosome level genome assembly of L. sceleratus and explored its evolutionary landscape. Through a phylogenomic analysis, we positioned L. sceleratus closer to T. nigroviridis, compared to other members of the family, while gene family evolution analysis revealed that genes associated with the immune response have experienced rapid expansion, providing a genetic basis for studying how L. sceleratus is able to achieve highly successful colonisation. Moreover, we found that voltage-gated sodium channel (NaV 1.4) mutations previously connected to tetrodotoxin resistance in other pufferfishes are not found in L. sceleratus, highlighting the complex evolution of this trait. The high-quality genome assembly built here is expected to set the ground for future studies on the species biology.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The Tetraodontidae family encompasses several species which attract scientific interest in terms of their ecology and evolution. The silver-cheeked toadfish (Lagocephalus sceleratus) is a well-known “invasive sprinter” that has invaded and spread, in less than a decade, throughout the Eastern and part of the Western Mediterranean Sea from the Red Sea through the Suez Canal. In this study, we built and analysed the first near-chromosome level genome assembly of L. sceleratus and explored its evolutionary landscape. Through a phylogenomic analysis, we positioned L. sceleratus closer to T. nigroviridis, compared to other members of the family, while gene family evolution analysis revealed that genes associated with the immune response have experienced rapid expansion, providing a genetic basis for studying how L. sceleratus is able to achieve highly successful colonisation. Moreover, we found that voltage-gated sodium channel (NaV 1.4) mutations previously connected to tetrodotoxin resistance in other pufferfishes are not found in L. sceleratus, highlighting the complex evolution of this trait. The high-quality genome assembly built here is expected to set the ground for future studies on the species biology. |
2020 |
Nousias, Orestis; Tsakogiannis, Alexandros; Duncan, Neil; Villa, Javier; Tzokas, Kostas; Estevez, Alicia; Chatziplis, Dimitrios; Tsigenopoulos, Costas S Parentage assignment, estimates of heritability and genetic correlation for growth-related traits in meagre Argyrosomus regius Journal Article Aquaculture, 518 , pp. 734663, 2020, ISSN: 00448486. @article{nousias_parentage_2020, title = {Parentage assignment, estimates of heritability and genetic correlation for growth-related traits in meagre Argyrosomus regius}, author = {Orestis Nousias and Alexandros Tsakogiannis and Neil Duncan and Javier Villa and Kostas Tzokas and Alicia Estevez and Dimitrios Chatziplis and Costas S Tsigenopoulos}, url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848619314346}, doi = {10.1016/j.aquaculture.2019.734663}, issn = {00448486}, year = {2020}, date = {2020-03-01}, urldate = {2020-08-18}, journal = {Aquaculture}, volume = {518}, pages = {734663}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Tsakogiannis, Alexandros; Manousaki, Tereza; Anagnostopoulou, Vasileia; Stavroulaki, Melanthia; Apostolaki, Eugenia T The Importance of Genomics for Deciphering the Invasion Success of the Seagrass Halophila stipulacea in the Changing Mediterranean Sea Journal Article Diversity, 12 (7), 2020, ISSN: 1424-2818. @article{tsakogiannis_importance_2020, title = {The Importance of Genomics for Deciphering the Invasion Success of the Seagrass Halophila stipulacea in the Changing Mediterranean Sea}, author = {Alexandros Tsakogiannis and Tereza Manousaki and Vasileia Anagnostopoulou and Melanthia Stavroulaki and Eugenia T Apostolaki}, url = {https://www.mdpi.com/1424-2818/12/7/263}, doi = {10.3390/d12070263}, issn = {1424-2818}, year = {2020}, date = {2020-01-01}, journal = {Diversity}, volume = {12}, number = {7}, abstract = {The Mediterranean Sea is subject to pressures from biological invasion due to coastal anthropic activities and global warming, which potentially modify its biogeography. The Red Sea tropical seagrass Halophila stipulacea entered the Eastern Mediterranean over a century ago, and its occurrence is expanding towards the northwest. Here, we highlight the importance of genomics for deciphering the evolutionary and ecological procedures taking place during the invasion process of H. stipulacea and review the relatively sparse genetic information available for the species to date. We report the first draft whole-genome sequencing of a H. stipulacea individual from Greece, based on Illumina Sequencing technology. A comparison of the Internal Transcribed Spacer (ITS) regions revealed a high divergence of the herein sequenced individual compared to Mediterranean populations sequenced two decades ago, rendering further questions on the evolutionary processes taking place during H. stipulacea adaptation in the invaded Mediterranean Sea. Our work sets the baseline for a future analysis of the invasion genomic of the focal species.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The Mediterranean Sea is subject to pressures from biological invasion due to coastal anthropic activities and global warming, which potentially modify its biogeography. The Red Sea tropical seagrass Halophila stipulacea entered the Eastern Mediterranean over a century ago, and its occurrence is expanding towards the northwest. Here, we highlight the importance of genomics for deciphering the evolutionary and ecological procedures taking place during the invasion process of H. stipulacea and review the relatively sparse genetic information available for the species to date. We report the first draft whole-genome sequencing of a H. stipulacea individual from Greece, based on Illumina Sequencing technology. A comparison of the Internal Transcribed Spacer (ITS) regions revealed a high divergence of the herein sequenced individual compared to Mediterranean populations sequenced two decades ago, rendering further questions on the evolutionary processes taking place during H. stipulacea adaptation in the invaded Mediterranean Sea. Our work sets the baseline for a future analysis of the invasion genomic of the focal species. |
2019 |
Natsidis, Paschalis; Tsakogiannis, Alexandros; Pavlidis, Pavlos; Tsigenopoulos, Costas S; Manousaki, Tereza Phylogenomics investigation of sparids (Teleostei: Spariformes) using high-quality proteomes highlights the importance of taxon sampling Journal Article Communications Biology, 2 (1), pp. 400, 2019, ISSN: 2399-3642. @article{natsidis_phylogenomics_2019, title = {Phylogenomics investigation of sparids (Teleostei: Spariformes) using high-quality proteomes highlights the importance of taxon sampling}, author = {Paschalis Natsidis and Alexandros Tsakogiannis and Pavlos Pavlidis and Costas S Tsigenopoulos and Tereza Manousaki}, url = {http://www.nature.com/articles/s42003-019-0654-5}, doi = {10.1038/s42003-019-0654-5}, issn = {2399-3642}, year = {2019}, date = {2019-12-01}, urldate = {2020-08-18}, journal = {Communications Biology}, volume = {2}, number = {1}, pages = {400}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Kyriakis, Dimitrios; Kanterakis, Alexandros; Manousaki, Tereza; Tsakogiannis, Alexandros; Tsagris, Michalis; Tsamardinos, Ioannis; Papaharisis, Leonidas; Chatziplis, Dimitris; Potamias, George; Tsigenopoulos, Costas S Scanning of Genetic Variants and Genetic Mapping of Phenotypic Traits in Gilthead Sea Bream Through ddRAD Sequencing Journal Article Frontiers in Genetics, 10 , pp. 675, 2019, ISSN: 1664-8021. @article{kyriakis_scanning_2019, title = {Scanning of Genetic Variants and Genetic Mapping of Phenotypic Traits in Gilthead Sea Bream Through ddRAD Sequencing}, author = {Dimitrios Kyriakis and Alexandros Kanterakis and Tereza Manousaki and Alexandros Tsakogiannis and Michalis Tsagris and Ioannis Tsamardinos and Leonidas Papaharisis and Dimitris Chatziplis and George Potamias and Costas S Tsigenopoulos}, url = {https://www.frontiersin.org/article/10.3389/fgene.2019.00675/full}, doi = {10.3389/fgene.2019.00675}, issn = {1664-8021}, year = {2019}, date = {2019-08-01}, urldate = {2020-08-18}, journal = {Frontiers in Genetics}, volume = {10}, pages = {675}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2018 |
Pauletto, Marianna; Manousaki, Tereza; Ferraresso, Serena; Babbucci, Massimiliano; Tsakogiannis, Alexandros; Louro, Bruno; Vitulo, Nicola; Quoc, Viet Ha; Carraro, Roberta; Bertotto, Daniela; Franch, Rafaella; Maroso, Francesco; Aslam, Muhammad L; Sonesson, Anna K; Simionati, Barbara; Malacrida, Giorgio; Cestaro, Alessandro; Caberlotto, Stefano; Sarropoulou, Elena; Mylonas, Costantinos C; Power, Deborah M; Patarnello, Tomaso; Canario, Adelino V M; Tsigenopoulos, Costas; Bargelloni, Luca Genomic analysis of Sparus aurata reveals the evolutionary dynamics of sex-biased genes in a sequential hermaphrodite fish Journal Article Communications Biology, 1 (1), pp. 119, 2018, ISSN: 2399-3642. @article{pauletto_genomic_2018, title = {Genomic analysis of Sparus aurata reveals the evolutionary dynamics of sex-biased genes in a sequential hermaphrodite fish}, author = {Marianna Pauletto and Tereza Manousaki and Serena Ferraresso and Massimiliano Babbucci and Alexandros Tsakogiannis and Bruno Louro and Nicola Vitulo and Viet Ha Quoc and Roberta Carraro and Daniela Bertotto and Rafaella Franch and Francesco Maroso and Muhammad L Aslam and Anna K Sonesson and Barbara Simionati and Giorgio Malacrida and Alessandro Cestaro and Stefano Caberlotto and Elena Sarropoulou and Costantinos C Mylonas and Deborah M Power and Tomaso Patarnello and Adelino V M Canario and Costas Tsigenopoulos and Luca Bargelloni}, url = {http://www.nature.com/articles/s42003-018-0122-7}, doi = {10.1038/s42003-018-0122-7}, issn = {2399-3642}, year = {2018}, date = {2018-12-01}, urldate = {2020-08-21}, journal = {Communications Biology}, volume = {1}, number = {1}, pages = {119}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Tsakogiannis, Alexandros; Manousaki, Tereza; Lagnel, Jacques; Papanikolaou, Nikolaos; Papandroulakis, Nikos; Mylonas, Constantinos C; Tsigenopoulos, Costas S The gene toolkit implicated in functional sex in Sparidae hermaphrodites: inferences from comparative transcriptomics Journal Article Frontiers in Genetics, 9 , pp. 749, 2018, (Publisher: Frontiers). @article{tsakogiannis_gene_2018, title = {The gene toolkit implicated in functional sex in Sparidae hermaphrodites: inferences from comparative transcriptomics}, author = {Alexandros Tsakogiannis and Tereza Manousaki and Jacques Lagnel and Nikolaos Papanikolaou and Nikos Papandroulakis and Constantinos C Mylonas and Costas S Tsigenopoulos}, year = {2018}, date = {2018-01-01}, journal = {Frontiers in Genetics}, volume = {9}, pages = {749}, note = {Publisher: Frontiers}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Tsakogiannis, A; Manousaki, T; Lagnel, J; Sterioti, A; Pavlidis, M; Papandroulakis, N; Mylonas, CC; Tsigenopoulos, CS The transcriptomic signature of different sexes in two protogynous hermaphrodites: Insights into the molecular network underlying sex phenotype in fish Journal Article Scientific reports, 8 (1), pp. 3564, 2018, (Publisher: Nature Publishing Group). @article{tsakogiannis_transcriptomic_2018, title = {The transcriptomic signature of different sexes in two protogynous hermaphrodites: Insights into the molecular network underlying sex phenotype in fish}, author = {A Tsakogiannis and T Manousaki and J Lagnel and A Sterioti and M Pavlidis and N Papandroulakis and CC Mylonas and CS Tsigenopoulos}, year = {2018}, date = {2018-01-01}, journal = {Scientific reports}, volume = {8}, number = {1}, pages = {3564}, note = {Publisher: Nature Publishing Group}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Manousaki, T; Tsakogiannis, A; Lagnel, J; Kyriakis, D; Duncan, N; Estevez, A; Tsigenopoulos, CS Muscle and liver transcriptome characterization and genetic marker discovery in the farmed meagre, Argyrosomus regius Journal Article Marine Genomics, 39 , pp. 39–44, 2018, (Publisher: Elsevier). @article{manousaki_muscle_2018, title = {Muscle and liver transcriptome characterization and genetic marker discovery in the farmed meagre, Argyrosomus regius}, author = {T Manousaki and A Tsakogiannis and J Lagnel and D Kyriakis and N Duncan and A Estevez and CS Tsigenopoulos}, doi = {10.1016/j.margen.2018.01.002}, year = {2018}, date = {2018-01-01}, journal = {Marine Genomics}, volume = {39}, pages = {39--44}, note = {Publisher: Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2016 |
Manousaki, Tereza; Tsakogiannis, Alexandros; Taggart, John B; Palaiokostas, Christos; Tsaparis, Dimitris; Lagnel, Jacques; Chatziplis, Dimitrios; Magoulas, Antonios; Papandroulakis, Nikos; Mylonas, Constantinos C; TSIGENOPOULOS, C S Exploring a nonmodel teleost genome through rad sequencing—linkage mapping in Common Pandora, Pagellus erythrinus and comparative genomic analysis Journal Article G3: Genes, genomes, genetics, 6 (3), pp. 509–519, 2016, (Publisher: G3: Genes, Genomes, Genetics). @article{manousaki_exploring_2016, title = {Exploring a nonmodel teleost genome through rad sequencing—linkage mapping in Common Pandora, Pagellus erythrinus and comparative genomic analysis}, author = {Tereza Manousaki and Alexandros Tsakogiannis and John B Taggart and Christos Palaiokostas and Dimitris Tsaparis and Jacques Lagnel and Dimitrios Chatziplis and Antonios Magoulas and Nikos Papandroulakis and Constantinos C Mylonas and C. S. TSIGENOPOULOS }, year = {2016}, date = {2016-01-01}, journal = {G3: Genes, genomes, genetics}, volume = {6}, number = {3}, pages = {509--519}, note = {Publisher: G3: Genes, Genomes, Genetics}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2015 |
Manousaki, T; Tsakogiannis, A; Taggart, B J; Chatziplis, D; Mylonas, C C; Tsigenopoulos, C S Linkage mapping in common pandora, Pagellus erythrinus, using ddRAD methodology Inproceedings 11th Panhellenic Symposium on Oceanography and Fisheries, pp. 69–72, Mytilene, Lesvos island, Greece, 2015, ISBN: 978-960-9798-08-2. @inproceedings{manousaki_linkage_2015, title = {Linkage mapping in common pandora, Pagellus erythrinus, using ddRAD methodology}, author = {T Manousaki and A Tsakogiannis and B J Taggart and D Chatziplis and C C Mylonas and C S Tsigenopoulos}, isbn = {978-960-9798-08-2}, year = {2015}, date = {2015-01-01}, booktitle = {11th Panhellenic Symposium on Oceanography and Fisheries}, pages = {69--72}, address = {Mytilene, Lesvos island, Greece}, abstract = {The common pandora (Pagellus erythrinus) is a relatively new species with interest for the aquaculture industry. The success of aquaculture depends on the availability of genomic information, including markers and linkage maps. We applied ddRAD methodology on a full-sib family of common pandora, to identify SNPs widely distributed in the genome. With the use of MiSeq platform, we sampled and sequenced a large genomic fraction that led to the discovery of 920 polymorphic loci. Linkage mapping analysis led to the construction of 24 linkage groups, highly homologous to stickleback genome. Our work exhibits the possibilities of new sequencing technologies in the study of cultured species.}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } The common pandora (Pagellus erythrinus) is a relatively new species with interest for the aquaculture industry. The success of aquaculture depends on the availability of genomic information, including markers and linkage maps. We applied ddRAD methodology on a full-sib family of common pandora, to identify SNPs widely distributed in the genome. With the use of MiSeq platform, we sampled and sequenced a large genomic fraction that led to the discovery of 920 polymorphic loci. Linkage mapping analysis led to the construction of 24 linkage groups, highly homologous to stickleback genome. Our work exhibits the possibilities of new sequencing technologies in the study of cultured species. |
2014 |
Karaiskou, N; Tsakogiannis, A; Gkagkavouzis, K; of Park, Operator Parnitha National; Papika, S; Latsoudis, P; Kavakiotis, I; Pantis, J; Abatzopoulos, T J; Triantaphyllidis, C; Triantafyllidis, A Greece: A Balkan Subrefuge for a Remnant Red Deer (Cervus Elaphus) Population Journal Article Journal of Heredity, 105 (3), pp. 334–344, 2014, ISSN: 0022-1503, 1465-7333. @article{karaiskou_greece_2014, title = {Greece: A Balkan Subrefuge for a Remnant Red Deer (Cervus Elaphus) Population}, author = {N Karaiskou and A Tsakogiannis and K Gkagkavouzis and Operator of Parnitha National Park and S Papika and P Latsoudis and I Kavakiotis and J Pantis and T J Abatzopoulos and C Triantaphyllidis and A Triantafyllidis}, url = {https://academic.oup.com/jhered/article-lookup/doi/10.1093/jhered/esu007}, doi = {10.1093/jhered/esu007}, issn = {0022-1503, 1465-7333}, year = {2014}, date = {2014-05-01}, urldate = {2020-08-18}, journal = {Journal of Heredity}, volume = {105}, number = {3}, pages = {334--344}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Manousaki, Tereza; Tsakogiannis, Alexandros; Lagnel, Jacques; Sarropoulou, Elena; Xiang, Jenny Z; Papandroulakis, Nikos; Mylonas, Constantinos C; Tsigenopoulos, Costas S The sex-specific transcriptome of the hermaphrodite sparid sharpsnout seabream (Diplodus puntazzo) Journal Article BMC genomics, 15 (1), pp. 655, 2014, (Publisher: BioMed Central). @article{manousaki_sex-specific_2014, title = {The sex-specific transcriptome of the hermaphrodite sparid sharpsnout seabream (Diplodus puntazzo)}, author = {Tereza Manousaki and Alexandros Tsakogiannis and Jacques Lagnel and Elena Sarropoulou and Jenny Z Xiang and Nikos Papandroulakis and Constantinos C Mylonas and Costas S Tsigenopoulos}, year = {2014}, date = {2014-01-01}, journal = {BMC genomics}, volume = {15}, number = {1}, pages = {655}, note = {Publisher: BioMed Central}, keywords = {}, pubstate = {published}, tppubtype = {article} } |