Publications

@article{kokokiris_induction_2025,

title = {Induction of Spawning in Captive Female Thicklip Grey Mullet (\textit{Chelon labrosus}) Treated with Different GnRHa Delivery Systems},

author = {Lambros E. Kokokiris and George Minos and Christos Simeonidis and Theofanis Karidas and Maria Papadaki and Constantinos C. Mylonas},

url = {https://www.mdpi.com/2410-3888/10/10/516

/wp-content/uploads/2025/11/2025-Kokokiris-FISHES-61.pdf

},

doi = {10.3390/fishes10100516},

issn = {2410-3888},

year  = {2025},

date = {2025-10-27},

urldate = {2025-10-27},

journal = {Fishes},

volume = {10},

number = {10},

pages = {516},

abstract = {Thick-lipped grey mullet (Chelon labrosus) has important characteristics that make it a promising candidate species for diversifying Mediterranean aquaculture. However, spontaneous spawning in captivity has not been documented, mainly due to failure of females to spawn, highlighting the need for further research on reproduction control. This study evaluated the efficacy of GnRHa administration, using repeated intramuscular injections or slow-release Ethylene–Vinyl Acetate (EVAc) implants combined with a dopamine antagonist (metoclopramide, Met), in terms of spawning performance and egg quality. Three groups were established: (a) saline injection (0.9% NaCl; Saline-INJ), (b) GnRHa [Des-Gly10, D-Ala6-ProNEth9-mGnRHa] injection at 10 μg kg−1 BW (GnRHa-INJ), and (c) EVAc implant containing GnRHa at 50 μg kg−1 bw (GnRHa-IMP). Over four weeks, Saline-INJ and GnRHa-INJ females received weekly saline or GnRHa, respectively. GnRHa-INJ and GnRHa-IMP females also received weekly Met (15 mg kg−1 bw). GnRHa induced 11 spawns (1,768,680 eggs), nearly triple the Saline-INJ group (4 spawns, 394,400 eggs). Daily relative fecundity (DRF) and fertilization success were highest in GnRHa-INJ (56,982 eggs kg−1 day−1; 59.7%), followed by GnRHa-IMP (20,375; 18.8%) and Saline-INJ (13,061; 9.1%). Multiple injections showed a trend toward higher spawning performance and egg quality compared to implants, although variability was high and further replication is needed. Nevertheless, optimizing both GnRHa delivery methods could further enhance their effectiveness while maintaining operational benefits for aquaculture.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{minguito-frutos_tropical_2025,

title = {Tropical range extending herbivorous fishes gain foraging benefits by shoaling with native temperate species},

author = {Mario Minguito-Frutos and Xavier Buñuel and Candela Marco-Méndez and Neus Sanmartí and Grigorios Skouradakis and Jordi Boada and Jordi F. Pagès and Teresa Alcoverro and Rohan Arthur},

url = {https://www.nature.com/articles/s41598-025-19136-x

/wp-content/uploads/2025/11/2025-Minguito-Frutos-SciRep-60.pdf

},

doi = {10.1038/s41598-025-19136-x},

issn = {2045-2322},

year  = {2025},

date = {2025-10-23},

urldate = {2025-10-08},

volume = {15},

number = {1},

pages = {35137},

abstract = {Abstract 

 Tropicalization creates novel species assemblages in temperate ecosystems as range-extending species move. The sociality of range-extending species could facilitate their establishment, particularly if they associate with natives. The eastern Mediterranean Sea has witnessed widespread collapses of vegetated ecosystems since the arrival of the tropical rabbitfishes 

 Siganus rivulatus 

 and 

 Siganus luridus 

 . We explored whether mixed-species shoaling influenced the fish foraging activity of the novel herbivore assemblage. We recorded 250 shoals, 30% composed exclusively of native species (i.e., 

 Sarpa salpa 

 and 

 Sparisoma cretense 

 ), 43% of rabbitfish, and 27% of species from both origins. 

 S. rivulatus 

 was the most gregarious species, establishing positive shoaling associations with 

 S. luridus 

 and 

 S. salpa 

 , whereas 

 S. cretense 

 was the least sociable. Foraging differed between native and range-extending species. Compared to native species in mixed-species shoals, range-extending species increased their bite rates with shoal size, both in mono- and multi-specific groups, indicating that they boosted their foraging efficiency (i.e., increased bites per minute) when foraging in large groups, regardless of which species they shoaled with. Native species, in contrast, only increased their bite rates with shoal size while foraging in mono-specific groups. Thus, our study shows that, unlike natives, range-extending species may gain foraging benefits from facilitative associations in mixed-species shoals. This gregarious behaviour could help explain the disproportionate herbivory pressures range-extending species impose on tropicalized ecosystems.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{rosso_unlocking_2025,

title = {Unlocking Mediterranean bryozoan diversity: seven new species unveiled after fixing a neotype for Fenestrulina malusii (Audouin & Savigny, 1826) (Cheilostomatida, Fenestrulinidae)},

author = {Antonietta Rosso and Emanuela Di Martino and Gemma Donato and Blanca Figuerola and Vasilis Gerovasileiou and Chiara Siddiolo and Alessandro Sinagra and Rossana Sanfilippo and Francesco Sciuto},

url = {https://zookeys.pensoft.net/article/157989/

/wp-content/uploads/2025/10/2025-Rosso-ZooKeys-58.pdf},

doi = {10.3897/zookeys.1254.157989},

issn = {1313-2970, 1313-2989},

year  = {2025},

date = {2025-10-15},

urldate = {2025-10-15},

journal = {ZooKeys},

volume = {1254},

pages = {1--74},

abstract = {Fenestrulina malusii 

 (Audouin & Savigny), the type species of 

 Fenestrulina 

 Jullien, is among the most widely reported bryozoans globally. Following the loss of the original type material, presumed Mediterranean in origin based on Savigny’s depiction on 

 Sargassum 

 , we designate a neotype from a colony on deep 

 Laminaria 

 blades off Ustica Island (Tyrrhenian Sea). Additionally, seven new species are described from various habitats across the Mediterranean: 

 F. cavernicola 

 sp. nov. 

 , from the semi-dark and dark zones of a submarine cave in Lesvos Island (NE Aegean Sea); 

 F. communis 

 sp. nov. 

 , from beach-cast plastic near Palermo (NW Sicily); 

 F. foveolata 

 sp. nov. 

 , from Ile-Rousse Bank (Corsica); 

 F. granulosa 

 sp. nov. 

 , from 

 Posidonia 

 meadows in Dhiaporia (Chios Island, Aegean Sea); 

 F. kalliste 

 sp. nov. 

 , from outer-shelf coarse sediments off Calvi (NW Corsica); 

 F. ovata 

 sp. nov. 

 , from Trémies submarine cave, near Marseille (Gulf of Lion); and 

 F. variorugosa 

 sp. nov. 

 , from 

 Posidonia 

 rootlets off Formica Isle (Egadi Archipelago, near the Sicily Strait). Five of these species are currently known only from their type localities, while 

 F. communis 

 sp. nov. 

 and 

 F. variorugosa 

 sp. nov. 

 occur elsewhere. An additional species with distinct features is described but left in open nomenclature owing to the absence of an ovicell. A comprehensive review of the published images of the 77 species currently assigned to 

 Fenestrulina 

 , with the identification of new diagnostic features, including kenozooids, has led to a revised diagnosis of the genus.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nunes_chemical_2025,

title = {Chemical profile of extracts from wild and farmed Chondrosia reniformis sponges},

author = {Maria João Nunes and Despoina Varamogianni-Mamatsi and Vanda Marques and Thekla I Anastasiou and Eirini Kagiampaki and Emmanouela Vernadou and Thanos Dailianis and Nicolas Kalogerakis and Luís C Branco and Cecília M P Rodrigues and Rita G Sobral and Manolis Mandalakis and Susana P Gaudêncio},

url = {https://link.springer.com/10.1007/s10499-025-02246-8

/wp-content/uploads/2025/10/2025-Nunes-AquaInt-57-preprint.pdf},

doi = {10.1007/s10499-025-02246-8},

issn = {0967-6120, 1573-143X},

year  = {2025},

date = {2025-10-13},

urldate = {2025-10-13},

journal = {Aquaculture International},

volume = {33},

number = {6},

pages = {558},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{bolanakis_weaving_2025,

title = {Weaving together the past, present and future of Greek arachnofauna: Biodiversity, knowledge gaps and conservation},

author = {Giannis Bolanakis and Savvas Paragkamian and Leonidas Maroulis and Panagiotis Kontos and Dimitris Poursanidis and Manolis Nikolakakis and Apostolos Trichas and Maria Chatzaki},

url = {https://resjournals.onlinelibrary.wiley.com/doi/10.1111/icad.70019

/wp-content/uploads/2025/10/2025-Volanakis-InsConsDiv-56-.pdf},

doi = {10.1111/icad.70019},

issn = {1752-458X, 1752-4598},

year  = {2025},

date = {2025-10-09},

urldate = {2025-10-09},

journal = {Insect Conservation and Diversity},

pages = {icad.70019},

abstract = {Abstract 

 

 

 

 Spiders are among the most species‐rich groups of arthropods. As top predators of other arthropods, their ecological role is significant, yet they are often overlooked in conservation efforts. Greece is home to a rich spider fauna, including many newly discovered and endemic taxa. 

 

 

 In this paper, we present an updated, comprehensive dataset of the spiders of Greece, compiled using open standards aligned with the FAIR principles of data management. This update is based on the critical curation of 187 papers covering all available records of spider species occurring in Greece. The curated data contain geographic location (including coordinates and their accuracy) and taxonomic validity of all species. 

 

 

 

 In total, 1310 species are recorded across more than 18,000 unique occurrences, excluding 

 nomina dubia 

 , subspecies and incomplete or erroneous taxonomic identifications. Of these, 226 species are endemic to Greece. Furthermore, an analysis is performed to present the distribution of the spider families/species richness in the complex landscape of Greece, in mainland and 96 islands, and the identified geographical/taxonomic gaps in our knowledge. 

 

 

 

 The conservation status of 202 IUCN‐assessed species is also presented, alongside a critical analysis of their documented threats and possible ways to promote their conservation.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{finn_establishing_2025,

title = {Establishing the ELIXIR Microbiome Community},

author = {Robert D Finn and Bachir Balech and Josephine Burgin and Physilia Chua and Erwan Corre and Cymon J Cox and Claudio Donati and Vitor Martins Dos Santos and Bruno Fosso and John Hancock and Katharina F Heil and Naveed Ishaque and Varsha Kale and Benoit J Kunath and Claudine Médigue and Teresa Nogueira and Evangelos Pafilis and Graziano Pesole and Lorna Richardson and Monica Santamaria and Nikolaos Strepis and Tim Van Den Bossche and Juan Antonio Vizcaíno and Haris Zafeiropoulos and Nils P Willassen and Eric Pelletier and Bérénice Batut},

url = {https://f1000research.com/articles/13-50/v2

/wp-content/uploads/2025/10/2025-Finn-F1000R-55.pdf},

doi = {10.12688/f1000research.144515.2},

issn = {2046-1402},

year  = {2025},

date = {2025-10-07},

urldate = {2025-10-07},

journal = {F1000Research},

volume = {13},

pages = {50},

abstract = {Microbiome research has grown substantially over the past decade in terms of the range of biomes sampled, identified taxa, and the volume of data derived from the samples. In particular, experimental approaches such as metagenomics, metabarcoding, metatranscriptomics and metaproteomics have provided profound insights into the vast, hitherto unknown, microbial biodiversity. The ELIXIR Marine Metagenomics Community, initiated amongst researchers focusing on marine microbiomes, has concentrated on promoting standards around microbiome-derived sequence analysis, as well as understanding the gaps in methods and reference databases, and identifying solutions to the computational overheads of performing such analyses. Nevertheless, the methods used and the challenges faced are not confined to marine microbiome studies, but are broadly applicable to other biomes. Thus, expanding this Marine Metagenomics Community to a more inclusive ELIXIR Microbiome Community will enable it to encompass a broader range of biomes and link expertise across ‘omics technologies. Furthermore, engaging with a large number of researchers will improve the efficiency and sustainability of bioinformatics infrastructure and resources for microbiome research (standards, data, tools, workflows, training), which will enable a deeper understanding of the function and taxonomic composition of the different microbial communities.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{rossi_marine_2025,

title = {Marine Animal Forests: Bridging Science, Policy, and Local Knowledge},

author = {Sergio Rossi and Lorenzo Bramanti and Federica Costantini and Thanos Dailianis and Nadine Schubert and Vasilis Gerovasileiou},

url = {https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/40440

/wp-content/uploads/2025/10/2025-Rossi-MMS-54.pdf},

doi = {10.12681/mms.40440},

issn = {1791-6763, 1108-393X},

year  = {2025},

date = {2025-10-02},

urldate = {2025-10-02},

journal = {Mediterranean Marine Science},

volume = {26},

number = {2},

pages = {418--420},

abstract = {Editorial 

Marine Animal Forests: Bridging Science, Policy, and Local Knowledge},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{ventriglia_histological_2025,

title = {Histological Insights into Testicular Apoptosis Associated with Spermatogenesis in Pre-Pubertal and Adult Meagre (\textit{Argyrosomus regius})},

author = {Gianluca Ventriglia and Neil Duncan and Ignacio Giménez and Constantinos C Mylonas and Chrysovalentinos Pousis and Caterina Varvara and Luisa Valentini and Letizia Passantino and Aldo Corriero and Rosa Zupa},

url = {https://www.mdpi.com/2076-2615/15/18/2668

/wp-content/uploads/2025/09/2025-Ventriglia-animals-53.pdf},

doi = {10.3390/ani15182668},

issn = {2076-2615},

year  = {2025},

date = {2025-09-29},

urldate = {2025-09-29},

journal = {Animals},

volume = {15},

number = {18},

pages = {2668},

abstract = {To overcome reproductive dysfunctions, recombinant gonadotropin hormones have been synthesised in different fish species. We were interested in gaining further insight into the effects of recombinant follicle-stimulating hormone (rFsh) in meagre Argyrosomus regius spermatogenesis and in improving the existing knowledge on the role of testicular apoptosis. Germ cell composition and testicular apoptosis were compared in pre-pubertal fish treated with rFsh (PreP-Fsh) and controls (PreP-C), and adult meagre with testes in the proliferative (Adult-Pro) and meiotic (Adult-Meio) spermatogenesis phases. A lower density of committed spermatogonia was observed in PreP-Fsh compared with PreP-C and in Adult-Meio compared with Adult-Pro. In pre-pubertal fish and in Adult-Pro, apoptosis affected mainly individual germ cells and Sertoli cells. In Adult-Meio, a high number of cysts containing apoptotic germ cells was observed, and apoptosis often involved entire clones of germ cells. rFsh reduced testicular apoptosis in pre-pubertal fish, whereas the advancement of spermatogenesis in adults was associated with an increased density of apoptotic cells. The present study corroborated previous observations on the effects of rFsh on spermatogenesis in pre-pubertal meagre and provided further insights on the changing role of apoptosis in the pre-pubertal phase versus different phases of spermatogenesis.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{racaku_complex_2025,

title = {Complex Sex Determination in the Grey Mullet Mugil cephalus Suggested by Individual Whole Genome Sequence Data},

author = {Mbarsid Racaku and Serena Ferraresso and Massimiliano Babbucci and Andres Blanco and Costas S Tsigenopoulos and Tereza Manousaki and Jelena Radojicic and Vasileios Papadogiannis and Paulino Martínez and Luca Bargelloni and Tomaso Patarnello},

url = {https://www.mdpi.com/2076-2615/15/16/2445

/wp-content/uploads/2025/09/2025-Racaku-ANIMALS-52.pdf},

doi = {10.3390/ani15162445},

issn = {2076-2615},

year  = {2025},

date = {2025-09-25},

urldate = {2025-09-25},

journal = {Animals},

volume = {15},

number = {16},

pages = {2445},

abstract = {Mugil cephalus is a cosmopolitan marine fish highly relevant from ecological and economic perspectives. Previous studies identified sex-associated variants in the follicle-stimulating hormone receptor (fshr) gene following an XX/XY sex determination (SD) system. However, these variants could not be fully associated with sex in all samples. This suggests other genes and/or environmental factors may be involved in the SD of this species, denoting intraspecific variation. In this study, we constructed a new high-quality genome assembly of M. cephalus. We then re-sequenced the whole genome in males and females from two divergent Mediterranean populations to ascertain whether other genetic variants could also be involved in SD. fshr gene variants showed to only partially explain SD, while a new intronic variant in the sestd1 gene appeared to be associated with SD following a ZZ/ZW system. The presence of other putative candidate SD variants showing significant differences between the two populations suggested a regional pattern of variation in SD in the Mediterranean Sea. The incomplete association of all the identified variants also pointed to a potential role for environmental factors.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{metcalfe_submarine_2025,

title = {Submarine ash megabed fed by far-traveled, shoreline-crossing pyroclastic currents from a large explosive volcanic eruption},

author = {Abigail Metcalfe and Tim Druitt and Katharina Pank and Steffen Kutterolf and Jonas Preine and Karim Kelfoun and Christian Hübscher and Paraskevi Nomikou and Thomas A Ronge and Shun Chiyonobu and Olga Koukousioura and Adam Woodhouse and Sarah Beethe and Michael Manga and Iona McIntosh and Masako Tominaga and Carole Berthod and Hehe Chen and Acacia Clark and Susan DeBari and Ralf Gertisser and Raymond Johnston and Ally Peccia and Yuzuru Yamamoto and Alexis Bernard and Tatiana Fernandez Perez and Christopher K Jones and Kumar Batuk Joshi and Günther Kletetschka and Molly McCanta and Antony Morris and Paraskevi Polymenakou and Xiaohui Li and Jean-Marie Nedelec and Hao-Yang Lee and Dimitrios Papanikolaou},

url = {https://www.science.org/doi/10.1126/sciadv.ads9642

/wp-content/uploads/2025/09/2025-Metcalfe-Sci-Adv-51.pdf},

doi = {10.1126/sciadv.ads9642},

issn = {2375-2548},

year  = {2025},

date = {2025-09-22},

urldate = {2025-09-22},

journal = {Science Advances},

volume = {11},

number = {33},

pages = {eads9642},

abstract = {Large explosive volcanic eruptions from island volcanoes deliver vast quantities of ash to the marine environment. While many of the transport pathways are understood, those from shoreline-crossing or submarine pyroclastic currents, and their transformation into water-supported gravity flows, remain poorly constrained. We report the discovery by International Ocean Discovery Program (IODP) deep drilling of a 200-meter-thick ash megabed buried in rift basins of the South Aegean Volcanic Arc. The textgreater73–cubic kilometer ash deposit originates from the Kos Plateau Tuff caldera-forming eruption, which occurred 161 thousand years ago, textgreater120 kilometers to the east. The ash forms a chemically uniform, graded megabed lacking bioturbation, interpreted as having been emplaced by a stream of eruption-fed turbidity currents. Bioclastic debris within the ash provides evidence of widespread destruction of marine ecosystems. Large volcanic eruptions can remodel the seafloor landscape, deposit thick ash turbidites, and destroy marine biota on island arc–wide scales in short-lived, catastrophic events. 

 , 

 Volcanic ash from an eruption in Greece poured into the sea, forming a bed 200 m thick in a submarine basin far from the volcano.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{gittings_remotely_2025,

title = {Remotely Sensing Phytoplankton Size Structure in the Mediterranean Sea: Insights from In Situ Data and Temperature-Corrected Abundance-Based Models},

author = {John A Gittings and Eleni Livanou and Xuerong Sun and Robert J W Brewin and Stella Psarra and Manolis Mandalakis and Alexandra Peltekis and Annalisa Di Cicco and Vittorio E Brando and Dionysios E Raitsos},

url = {https://www.mdpi.com/2072-4292/17/14/2362

/wp-content/uploads/2025/09/2025-Gittings-RemSense-50.pdf},

doi = {10.3390/rs17142362},

issn = {2072-4292},

year  = {2025},

date = {2025-09-15},

urldate = {2025-09-16},

journal = {Remote Sensing},

volume = {17},

number = {14},

pages = {2362},

abstract = {Since the mid-1980s, the Mediterranean Sea’s surface and deeper layers have warmed at unprecedented rates, with recent projections identifying it as one of the regions most impacted by rising global temperatures. Metrics that characterize phytoplankton abundance, phenology and size structure are widely utilized as ecological indicators that enable a quantitative assessment of the status of marine ecosystems in response to environmental change. Here, using an extensive, updated in situ pigment dataset collated from numerous past research campaigns across the Mediterranean Sea, we re-parameterized an abundance-based phytoplankton size class model that infers Chl-a concentration in three phytoplankton size classes: pico- (textless2 μm), nano- (2–20 μm) and micro-phytoplankton (textgreater20 μm). Following recent advancements made within this category of size class models, we also incorporated information of sea surface temperature (SST) into the model parameterization. By tying model parameters to SST, the performance of the re-parameterized model was improved based on comparisons with concurrent, independent in situ measurements. Similarly, the application of the model to remotely sensed ocean color observations revealed strong agreement between satellite-derived estimates of phytoplankton size structure and in situ observations, with a performance comparable to the current regional operational datasets on size structure. The proposed conceptual regional model, parameterized with the most extended in situ pigment dataset available to date for the area, serves as a suitable foundation for long-term (1997–present) analyses on phytoplankton size structure and ecological indicators (i.e., phenology), ultimately linking higher trophic level responses to a changing Mediterranean Sea.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{digenis_assessing_2025,

title = {Assessing the Motile Fauna of Eastern Mediterranean Marine Caves},

author = {Markos Digenis and Michail Ragkousis and Charalampos Dimitriadis and Stelios Katsanevakis and Vasilis Gerovasileiou},

url = {https://www.mdpi.com/2410-3888/10/8/383

/wp-content/uploads/2025/09/2025-Digenis-fishes-49.pdf},

doi = {10.3390/fishes10080383},

issn = {2410-3888},

year  = {2025},

date = {2025-09-12},

urldate = {2025-09-15},

journal = {Fishes},

volume = {10},

number = {8},

pages = {383},

abstract = {Although marine caves are among the most species-diverse habitats in the Mediterranean Sea, most available studies have focused on their sessile fauna. This study provides the first quantitative assessment of motile fauna in 27 marine caves across four geographical subareas of the Aegean and Ionian Seas, using a rapid assessment visual census protocol, applied through 3 min time transects in each ecological cave zone. Multivariate analysis revealed that the motile community structure of the cave entrance was differentiated from that of the semidark and dark zones. Deeper caves were distinct from shallower ones while caves of the east Aegean differed from those around Crete Island. A total of 163 taxa were recorded, 27 of which are reported herein for the first time in marine caves of the eastern Mediterranean Sea, while three species (two native and one introduced) are recorded in Greek waters for the first time, enriching our knowledge on the permanent and occasional cave residents. Seventeen species were introduced, comprising more than half of the total fish abundance in the southeasternmost cave. Our limited knowledge of the motile fauna of Mediterranean marine caves coupled with the continued spread of introduced species highlights the urgent need for monitoring and conservation actions, especially within marine protected areas.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{tampou_growth_2025,

title = {Growth Performance of Gilthead Sea Bream ( \textit{Sparus aurata} ) Fed Low Fish Meal Diets With an Innovative Mixture of Low Trophic Ingredients},

author = {Anna Tampou and Katerina Kousoulaki and Antigoni Vasilaki and Nikolaos Vlahos and Eleni Nikouli and Nikolas Panteli and Konstantinos Feidantsis and Konstantinos Kormas and Styliani Andreopoulou and Ioannis T Karapanagiotidis and Panagiotis Berillis and Ioannis Nengas and Efthimia Antonopoulou and Eleni Mente},

editor = {Noah Esmaeili},

url = {https://onlinelibrary.wiley.com/doi/10.1155/anu/7504207

/wp-content/uploads/2025/09/2025-Tampou-Aqua-Nutr-48.pdf},

doi = {10.1155/anu/7504207},

issn = {1353-5773, 1365-2095},

year  = {2025},

date = {2025-09-11},

urldate = {2025-09-12},

journal = {Aquaculture Nutrition},

volume = {2025},

number = {1},

pages = {7504207},

abstract = {This study examined the growth performance, cellular signaling, and gut microbiome of gilthead sea bream ( 

 Sparus aurata 

 ) fed four diets using low‐trophic‐level ingredients for 65 days. Control (C) diet contained fish meal (FM) as the main protein source and fish oil (FO) as a lipid source. In the 0%FMFO diet all FM and FO present in the C diet was replaced with a combination of microalgae, insect meal (IM), and tunicate meal (TM). IM and TM diets were formulated to contain 20% of the novel protein ingredients, replacing 68.09% and 45.91% of FM in diet C, respectively. Compared to diet C, feed utilization and growth performance of 

 S 

 . 

 aurata 

 fed 0%FMFO diet was not different ( 

 p 

 textgreater 0.05) and TM diet had a significantly lower ( 

 p 

   textless 0.05) specific growth rate (SGR), higher ( 

 p 

   textless 0.05) feed conversion ratio (FCR) and decreased fillet lipid content ( 

 p 

 textless 0.05). In liver and foregut of fish fed TM diet the activity of lactate dehydrogenase (L‐LDH) significant increased ( 

 p 

 textless 0.05), indicating higher glycolytic potential, whereas the decrease in Hsp70, Hsp90, AMPK, and p38 MAPK may indicate reduced stress response. Fish midgut microbiome included beneficial taxa for the host. The results suggested that the mixture of algae, insect, and TM could replace all FM and FO in gilthead sea bream diets without affecting the fish growth performance.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{christou_climate_2025,

title = {Climate change in the “vulnerable” Eastern Mediterranean and adjacent areas: A literature review of ecological impacts and threats},

author = {Epaminondas D Christou and Thanos Dailianis and Evangelia Chatzinikolaou and Paraskevi K Karachle and Dimitrios Damalas and Konstantinos Tsagarakis and Evangelia Strogyloudi and Georgios Karris and Soultana Zervoudaki and Antonia Giannakourou and Maria Salomidi and Sofia Reizopoulou},

url = {https://linkinghub.elsevier.com/retrieve/pii/S0141113625004477

/wp-content/uploads/2025/09/2025-Christou-MarEnvRes-47.pdf},

doi = {10.1016/j.marenvres.2025.107390},

issn = {01411136},

year  = {2025},

date = {2025-09-08},

urldate = {2025-09-09},

journal = {Marine Environmental Research},

volume = {211},

pages = {107390},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{holm_first_2025,

title = {First island-wide, single-day soil collection study on Crete reveals environmental drivers of microbial diversity},

author = {Johanna B Holm and Savvas Paragkamian and Mike Humphreys and Apaala Chatterjee and Stephanie Yarwood and Josh Gaimaro and Melanthia Stavroulaki and Dimitris Tsaparis and Manolis Plaitis and Panagiotis Kasapidis and Stelios Darivianakis and Georgios Kotoulas and Antonios Magoulas and Anastasis Oulas and Zacharias Kyrpiotakis and Pavlos Pavlidis and Petra Ten Hoopen and Guy Cochrane and Wiebe H C F Kooistra and Jason R Schriml and Bronwen E Schriml and Ilias Lagkouvardos and Panos Gkorezis and Neil Davies and Christine Laney and Lee Stanish and Granger Sutton and Scott Tighe and Ilene Karsch Mizrachi and Donovan Parks and Pelin Yilmaz and Chelsea Carey and Pier Buttigieg and Philip Goldstein and Evangelos Pafilis and Lynn M Schriml},

url = {https://environmentalmicrobiome.biomedcentral.com/articles/10.1186/s40793-025-00752-z

/wp-content/uploads/2025/09/2025-Holm-Paragkamian-EnvMicro-46.pdf},

doi = {10.1186/s40793-025-00752-z},

issn = {2524-6372},

year  = {2025},

date = {2025-09-04},

urldate = {2025-09-08},

journal = {Environmental Microbiome},

volume = {20},

number = {1},

pages = {94},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{rotter_innovative_2025,

title = {Innovative solutions for valorization of desalination brine},

author = {Ana Rotter and Baruch Rinkevich and Irem Deniz and Maggie M Reddy and Mariana Girão and Maria F Carvalho and Nina Gunde-Cimerman and Cene Gostinčar and Mercedes Cueto and Ana R Díaz-Marrero and Viktoriia Komarysta and Fusun Akgul and Lada Lukić Bilela and Ernesta Grigalionyte-Bembič and Manolis Mandalakis},

url = {https://linkinghub.elsevier.com/retrieve/pii/S2589914725000714

/wp-content/uploads/2025/09/2025-Rotter-WaterRX-45.pdf},

doi = {10.1016/j.wroa.2025.100372},

issn = {25899147},

year  = {2025},

date = {2025-09-01},

urldate = {2025-09-05},

journal = {Water Research X},

volume = {28},

pages = {100372},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{stavrakidis-zachou_nutritional_2025,

title = {A Nutritional Bioenergetic Model for Farmed Fish: Effects of Food Composition on Growth, Oxygen Consumption and Waste Production},

author = {Orestis Stavrakidis-Zachou and Ep H Eding and Nikos Papandroulakis and Konstadia Lika},

editor = {Liqiao Chen},

url = {https://onlinelibrary.wiley.com/doi/10.1155/anu/9010939

/wp-content/uploads/2025/09/2025-Stavrakidis-Zachou-AquaNutr-44.pdf},

doi = {10.1155/anu/9010939},

issn = {1353-5773, 1365-2095},

year  = {2025},

date = {2025-08-29},

urldate = {2025-09-04},

journal = {Aquaculture Nutrition},

volume = {2025},

number = {1},

pages = {9010939},

abstract = {The study of flow and transformation of energy and nutrients via mathematical modelling provides an in silico tool approach for designing scientific experiments, improving precision in aquaculture production and reducing the need for experimental animals. The proposed nutritional bioenergetics model is based on the dynamic energy budget (DEB) theory, a mechanistic framework to study individual metabolism. The model is an extension of the typical DEB models in that it includes a digestion module where the protein and non‐protein food components contribute to assimilation via the concept of a synthesising unit (SU). The model allows predictions for measurable quantities of interest for aquaculture, including feeding rate, oxygen consumption, carbon dioxide, ammonia and solid waste production, under various temperatures and feeding conditions, both in terms of quantity and macronutrient composition. The feeding schedule’s effects, such as the diurnal variation in waste production in response to feeding frequency, are also captured. The model quantifies the effects of the dietary protein‐to‐energy ratio on food intake and assimilation; energy‐rich diets or those with excessive or poor amounts of protein show reduced intake. The model has been parametrised and validated for rainbow trout ( 

 Oncorhynchus mykiss 

 ) to demonstrate its capabilities. Testing the model with diverse datasets has shown that it predicts weight gain well, and to a lesser extent, oxygen consumption and total ammonia production. The proposed model could be a useful in silico tool for fish researchers, technicians and farm operators.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{conides_innovative_2025,

title = {An Innovative Metal–Synthetic Hybrid Thread for the Construction of Aquaculture Nets},

author = {Alexis Conides and Efthimia Cotou and Dimitris Klaoudatos and Branko Glamuzina},

url = {https://www.mdpi.com/2077-1312/13/8/1384

/wp-content/uploads/2025/09/2025-Conides-MSciEng-43.pdf},

doi = {10.3390/jmse13081384},

issn = {2077-1312},

year  = {2025},

date = {2025-08-27},

urldate = {2025-09-03},

journal = {Journal of Marine Science and Engineering},

volume = {13},

number = {8},

pages = {1384},

abstract = {Based on the experience gained worldwide from potential solutions to the fouling problem of fisheries and aquaculture infrastructure, we attempted to design, construct and test the antifouling efficiency of a new hybrid filament created from non-laminated copper wire braided with synthetic fibers made of Dyneema. The design involved the creation of a hybrid twine substituting a percentage of the synthetic fibers with 0.1–0.15 mm diameter copper wire at 5%, 10%, 20% and 40% levels. There is limited information in the international literature for comparison with our results, since there has never been any attempt to create such a hybrid net. The results showed that for the 6 mm mesh, the maximum openness obtained after the 8-month experimental period was 8.72%, with Cu wire substitution at 35%. For the 12 mm mesh, these values were 27.07% at 26%, and for the 20 mm mesh, they were 33.68% at 28%. A conservative average independent from mesh size to achieve optimum openness in the long term is 30 ± 4.73% Cu wire substitution. In addition, we found that both the mesh size (mm) and the copper substitution percentage affected the fouling process during the experimental period, which lasted 8 months.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{salomidi_advancing_2025,

title = {Advancing knowledge on red coral Corallium rubrum (Linnaeus, 1758) populations and associated mesophotic communities in the Aegean Sea, Eastern Mediterranean},

author = {Maria Salomidi and Alfredo Marchiò and Yiannis Issaris and Vasilis Gerakaris and Thanos Dailianis and Vasilis Gerovasileiou},

url = {https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/41403

/wp-content/uploads/2025/09/2025-Salomidi-MMS-42.pdf

},

doi = {10.12681/mms.41403},

issn = {1791-6763, 1108-393X},

year  = {2025},

date = {2025-08-25},

urldate = {2025-09-02},

journal = {Mediterranean Marine Science},

volume = {26},

number = {2},

pages = {378--392},

abstract = {This study presents the first quantitative analysis of live red coral (Corallium rubrum) populations in Greek waters (North-Western Aegean Sea) and contributes to the limited knowledge of mesophotic benthic assemblages in the Eastern Mediterranean Sea. Utilizing ROV and stereo-video SCUBA surveys at two previously harvested sites (47-60 m depth), we quantified red coral density and morphometric parameters. Associated benthic communities, comprising 101 taxa with Porifera as the dominant group, were characterized, and the Mesophotic Assemblages Conservation Status (MACS) index was applied for the first time in this region, revealing an overall “Good” ecological status despite locally degraded facies. While red coral entanglement by widespread ghost fishing gear appeared low, high frequencies of necrosis and epibiosis were observed in the larger canopy-forming gorgonian Paramuricea clavata, potentially indicating current and past mechanical damage, although the synergistic effects of other physical stressors cannot be excluded. This research offers crucial baseline data on red coral populations and associated mesophotic communities in an under-studied region and ecological zone. Our findings are discussed in the context of existing national management frameworks and conservation measures, emphasizing the need for further monitoring and research to inform effective management and conservation strategies in view of increasing anthropogenic and environmental pressures.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{angelakopoulos_effects_2025,

title = {The Effects of Early Temperature and Live Feeds on the Development of White Muscle in Greater Amberjack (Seriola dumerili)},

author = {Rafael Angelakopoulos and Andreas Tsipourlianos and Alexia E Fytsili and Nikolaos Mitrizakis and Themistoklis Giannoulis and Nikos Papandroulakis and Katerina A Moutou},

url = {https://www.mdpi.com/2410-3888/10/7/360

/wp-content/uploads/2025/08/2025-Angelakopoulos-Fishes-41.pdf},

doi = {10.3390/fishes10070360},

issn = {2410-3888},

year  = {2025},

date = {2025-08-11},

urldate = {2025-08-11},

journal = {Fishes},

volume = {10},

number = {7},

pages = {360},

abstract = {Greater amberjack (Seriola dumerili) shows potential for Mediterranean aquaculture due to its swift growth, consumer appeal, and commercial value. However, challenges in juvenile production, such as growth dispersion and unsynchronized development, impede further expansion. This study explores the impact of rearing temperature and live feed types on early white muscle development in greater amberjack larvae. Findings reveal substantial effects of temperature and diet on larval development, highlighting that the combination of 24 °C and a copepod + rotifer co-feeding scheme resulted in the highest axial growth rate, whereas rotifer-fed larvae at 20 °C exhibited a slower pace. Incorporating both histological and gene expression analyses, the study underscores temperature’s significant influence on white muscle development. Among larvae reared at 24 °C, the two live feed types led to phenotypic variations at metamorphosis, with rotifers supporting longer larvae featuring a smaller total cross-sectional area compared to copepods. Gene expression analysis indicates heightened mylpfb and myog expression at 24 °C during early larval stages, suggesting increased hyperplasia and myoblast differentiation. This study highlights the necessity of considering both temperature and feed type in larval rearing practices for optimal muscle development, and further research exploring combined diets during rearing could offer insights to enhance amberjack aquaculture sustainability.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{kokokiris_induction_2025,

title = {Induction of Spawning in Captive Female Thicklip Grey Mullet (\textit{Chelon labrosus}) Treated with Different GnRHa Delivery Systems},

author = {Lambros E. Kokokiris and George Minos and Christos Simeonidis and Theofanis Karidas and Maria Papadaki and Constantinos C. Mylonas},

url = {https://www.mdpi.com/2410-3888/10/10/516

/wp-content/uploads/2025/11/2025-Kokokiris-FISHES-61.pdf

},

doi = {10.3390/fishes10100516},

issn = {2410-3888},

year  = {2025},

date = {2025-10-27},

urldate = {2025-10-27},

journal = {Fishes},

volume = {10},

number = {10},

pages = {516},

abstract = {Thick-lipped grey mullet (Chelon labrosus) has important characteristics that make it a promising candidate species for diversifying Mediterranean aquaculture. However, spontaneous spawning in captivity has not been documented, mainly due to failure of females to spawn, highlighting the need for further research on reproduction control. This study evaluated the efficacy of GnRHa administration, using repeated intramuscular injections or slow-release Ethylene–Vinyl Acetate (EVAc) implants combined with a dopamine antagonist (metoclopramide, Met), in terms of spawning performance and egg quality. Three groups were established: (a) saline injection (0.9% NaCl; Saline-INJ), (b) GnRHa [Des-Gly10, D-Ala6-ProNEth9-mGnRHa] injection at 10 μg kg−1 BW (GnRHa-INJ), and (c) EVAc implant containing GnRHa at 50 μg kg−1 bw (GnRHa-IMP). Over four weeks, Saline-INJ and GnRHa-INJ females received weekly saline or GnRHa, respectively. GnRHa-INJ and GnRHa-IMP females also received weekly Met (15 mg kg−1 bw). GnRHa induced 11 spawns (1,768,680 eggs), nearly triple the Saline-INJ group (4 spawns, 394,400 eggs). Daily relative fecundity (DRF) and fertilization success were highest in GnRHa-INJ (56,982 eggs kg−1 day−1; 59.7%), followed by GnRHa-IMP (20,375; 18.8%) and Saline-INJ (13,061; 9.1%). Multiple injections showed a trend toward higher spawning performance and egg quality compared to implants, although variability was high and further replication is needed. Nevertheless, optimizing both GnRHa delivery methods could further enhance their effectiveness while maintaining operational benefits for aquaculture.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{minguito-frutos_tropical_2025,

title = {Tropical range extending herbivorous fishes gain foraging benefits by shoaling with native temperate species},

author = {Mario Minguito-Frutos and Xavier Buñuel and Candela Marco-Méndez and Neus Sanmartí and Grigorios Skouradakis and Jordi Boada and Jordi F. Pagès and Teresa Alcoverro and Rohan Arthur},

url = {https://www.nature.com/articles/s41598-025-19136-x

/wp-content/uploads/2025/11/2025-Minguito-Frutos-SciRep-60.pdf

},

doi = {10.1038/s41598-025-19136-x},

issn = {2045-2322},

year  = {2025},

date = {2025-10-23},

urldate = {2025-10-08},

volume = {15},

number = {1},

pages = {35137},

abstract = {Abstract 

 Tropicalization creates novel species assemblages in temperate ecosystems as range-extending species move. The sociality of range-extending species could facilitate their establishment, particularly if they associate with natives. The eastern Mediterranean Sea has witnessed widespread collapses of vegetated ecosystems since the arrival of the tropical rabbitfishes 

 Siganus rivulatus 

 and 

 Siganus luridus 

 . We explored whether mixed-species shoaling influenced the fish foraging activity of the novel herbivore assemblage. We recorded 250 shoals, 30% composed exclusively of native species (i.e., 

 Sarpa salpa 

 and 

 Sparisoma cretense 

 ), 43% of rabbitfish, and 27% of species from both origins. 

 S. rivulatus 

 was the most gregarious species, establishing positive shoaling associations with 

 S. luridus 

 and 

 S. salpa 

 , whereas 

 S. cretense 

 was the least sociable. Foraging differed between native and range-extending species. Compared to native species in mixed-species shoals, range-extending species increased their bite rates with shoal size, both in mono- and multi-specific groups, indicating that they boosted their foraging efficiency (i.e., increased bites per minute) when foraging in large groups, regardless of which species they shoaled with. Native species, in contrast, only increased their bite rates with shoal size while foraging in mono-specific groups. Thus, our study shows that, unlike natives, range-extending species may gain foraging benefits from facilitative associations in mixed-species shoals. This gregarious behaviour could help explain the disproportionate herbivory pressures range-extending species impose on tropicalized ecosystems.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{rosso_unlocking_2025,

title = {Unlocking Mediterranean bryozoan diversity: seven new species unveiled after fixing a neotype for Fenestrulina malusii (Audouin & Savigny, 1826) (Cheilostomatida, Fenestrulinidae)},

author = {Antonietta Rosso and Emanuela Di Martino and Gemma Donato and Blanca Figuerola and Vasilis Gerovasileiou and Chiara Siddiolo and Alessandro Sinagra and Rossana Sanfilippo and Francesco Sciuto},

url = {https://zookeys.pensoft.net/article/157989/

/wp-content/uploads/2025/10/2025-Rosso-ZooKeys-58.pdf},

doi = {10.3897/zookeys.1254.157989},

issn = {1313-2970, 1313-2989},

year  = {2025},

date = {2025-10-15},

urldate = {2025-10-15},

journal = {ZooKeys},

volume = {1254},

pages = {1--74},

abstract = {Fenestrulina malusii 

 (Audouin & Savigny), the type species of 

 Fenestrulina 

 Jullien, is among the most widely reported bryozoans globally. Following the loss of the original type material, presumed Mediterranean in origin based on Savigny’s depiction on 

 Sargassum 

 , we designate a neotype from a colony on deep 

 Laminaria 

 blades off Ustica Island (Tyrrhenian Sea). Additionally, seven new species are described from various habitats across the Mediterranean: 

 F. cavernicola 

 sp. nov. 

 , from the semi-dark and dark zones of a submarine cave in Lesvos Island (NE Aegean Sea); 

 F. communis 

 sp. nov. 

 , from beach-cast plastic near Palermo (NW Sicily); 

 F. foveolata 

 sp. nov. 

 , from Ile-Rousse Bank (Corsica); 

 F. granulosa 

 sp. nov. 

 , from 

 Posidonia 

 meadows in Dhiaporia (Chios Island, Aegean Sea); 

 F. kalliste 

 sp. nov. 

 , from outer-shelf coarse sediments off Calvi (NW Corsica); 

 F. ovata 

 sp. nov. 

 , from Trémies submarine cave, near Marseille (Gulf of Lion); and 

 F. variorugosa 

 sp. nov. 

 , from 

 Posidonia 

 rootlets off Formica Isle (Egadi Archipelago, near the Sicily Strait). Five of these species are currently known only from their type localities, while 

 F. communis 

 sp. nov. 

 and 

 F. variorugosa 

 sp. nov. 

 occur elsewhere. An additional species with distinct features is described but left in open nomenclature owing to the absence of an ovicell. A comprehensive review of the published images of the 77 species currently assigned to 

 Fenestrulina 

 , with the identification of new diagnostic features, including kenozooids, has led to a revised diagnosis of the genus.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nunes_chemical_2025,

title = {Chemical profile of extracts from wild and farmed Chondrosia reniformis sponges},

author = {Maria João Nunes and Despoina Varamogianni-Mamatsi and Vanda Marques and Thekla I Anastasiou and Eirini Kagiampaki and Emmanouela Vernadou and Thanos Dailianis and Nicolas Kalogerakis and Luís C Branco and Cecília M P Rodrigues and Rita G Sobral and Manolis Mandalakis and Susana P Gaudêncio},

url = {https://link.springer.com/10.1007/s10499-025-02246-8

/wp-content/uploads/2025/10/2025-Nunes-AquaInt-57-preprint.pdf},

doi = {10.1007/s10499-025-02246-8},

issn = {0967-6120, 1573-143X},

year  = {2025},

date = {2025-10-13},

urldate = {2025-10-13},

journal = {Aquaculture International},

volume = {33},

number = {6},

pages = {558},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{bolanakis_weaving_2025,

title = {Weaving together the past, present and future of Greek arachnofauna: Biodiversity, knowledge gaps and conservation},

author = {Giannis Bolanakis and Savvas Paragkamian and Leonidas Maroulis and Panagiotis Kontos and Dimitris Poursanidis and Manolis Nikolakakis and Apostolos Trichas and Maria Chatzaki},

url = {https://resjournals.onlinelibrary.wiley.com/doi/10.1111/icad.70019

/wp-content/uploads/2025/10/2025-Volanakis-InsConsDiv-56-.pdf},

doi = {10.1111/icad.70019},

issn = {1752-458X, 1752-4598},

year  = {2025},

date = {2025-10-09},

urldate = {2025-10-09},

journal = {Insect Conservation and Diversity},

pages = {icad.70019},

abstract = {Abstract 

 

 

 

 Spiders are among the most species‐rich groups of arthropods. As top predators of other arthropods, their ecological role is significant, yet they are often overlooked in conservation efforts. Greece is home to a rich spider fauna, including many newly discovered and endemic taxa. 

 

 

 In this paper, we present an updated, comprehensive dataset of the spiders of Greece, compiled using open standards aligned with the FAIR principles of data management. This update is based on the critical curation of 187 papers covering all available records of spider species occurring in Greece. The curated data contain geographic location (including coordinates and their accuracy) and taxonomic validity of all species. 

 

 

 

 In total, 1310 species are recorded across more than 18,000 unique occurrences, excluding 

 nomina dubia 

 , subspecies and incomplete or erroneous taxonomic identifications. Of these, 226 species are endemic to Greece. Furthermore, an analysis is performed to present the distribution of the spider families/species richness in the complex landscape of Greece, in mainland and 96 islands, and the identified geographical/taxonomic gaps in our knowledge. 

 

 

 

 The conservation status of 202 IUCN‐assessed species is also presented, alongside a critical analysis of their documented threats and possible ways to promote their conservation.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{finn_establishing_2025,

title = {Establishing the ELIXIR Microbiome Community},

author = {Robert D Finn and Bachir Balech and Josephine Burgin and Physilia Chua and Erwan Corre and Cymon J Cox and Claudio Donati and Vitor Martins Dos Santos and Bruno Fosso and John Hancock and Katharina F Heil and Naveed Ishaque and Varsha Kale and Benoit J Kunath and Claudine Médigue and Teresa Nogueira and Evangelos Pafilis and Graziano Pesole and Lorna Richardson and Monica Santamaria and Nikolaos Strepis and Tim Van Den Bossche and Juan Antonio Vizcaíno and Haris Zafeiropoulos and Nils P Willassen and Eric Pelletier and Bérénice Batut},

url = {https://f1000research.com/articles/13-50/v2

/wp-content/uploads/2025/10/2025-Finn-F1000R-55.pdf},

doi = {10.12688/f1000research.144515.2},

issn = {2046-1402},

year  = {2025},

date = {2025-10-07},

urldate = {2025-10-07},

journal = {F1000Research},

volume = {13},

pages = {50},

abstract = {Microbiome research has grown substantially over the past decade in terms of the range of biomes sampled, identified taxa, and the volume of data derived from the samples. In particular, experimental approaches such as metagenomics, metabarcoding, metatranscriptomics and metaproteomics have provided profound insights into the vast, hitherto unknown, microbial biodiversity. The ELIXIR Marine Metagenomics Community, initiated amongst researchers focusing on marine microbiomes, has concentrated on promoting standards around microbiome-derived sequence analysis, as well as understanding the gaps in methods and reference databases, and identifying solutions to the computational overheads of performing such analyses. Nevertheless, the methods used and the challenges faced are not confined to marine microbiome studies, but are broadly applicable to other biomes. Thus, expanding this Marine Metagenomics Community to a more inclusive ELIXIR Microbiome Community will enable it to encompass a broader range of biomes and link expertise across ‘omics technologies. Furthermore, engaging with a large number of researchers will improve the efficiency and sustainability of bioinformatics infrastructure and resources for microbiome research (standards, data, tools, workflows, training), which will enable a deeper understanding of the function and taxonomic composition of the different microbial communities.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{rossi_marine_2025,

title = {Marine Animal Forests: Bridging Science, Policy, and Local Knowledge},

author = {Sergio Rossi and Lorenzo Bramanti and Federica Costantini and Thanos Dailianis and Nadine Schubert and Vasilis Gerovasileiou},

url = {https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/40440

/wp-content/uploads/2025/10/2025-Rossi-MMS-54.pdf},

doi = {10.12681/mms.40440},

issn = {1791-6763, 1108-393X},

year  = {2025},

date = {2025-10-02},

urldate = {2025-10-02},

journal = {Mediterranean Marine Science},

volume = {26},

number = {2},

pages = {418--420},

abstract = {Editorial 

Marine Animal Forests: Bridging Science, Policy, and Local Knowledge},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{ventriglia_histological_2025,

title = {Histological Insights into Testicular Apoptosis Associated with Spermatogenesis in Pre-Pubertal and Adult Meagre (\textit{Argyrosomus regius})},

author = {Gianluca Ventriglia and Neil Duncan and Ignacio Giménez and Constantinos C Mylonas and Chrysovalentinos Pousis and Caterina Varvara and Luisa Valentini and Letizia Passantino and Aldo Corriero and Rosa Zupa},

url = {https://www.mdpi.com/2076-2615/15/18/2668

/wp-content/uploads/2025/09/2025-Ventriglia-animals-53.pdf},

doi = {10.3390/ani15182668},

issn = {2076-2615},

year  = {2025},

date = {2025-09-29},

urldate = {2025-09-29},

journal = {Animals},

volume = {15},

number = {18},

pages = {2668},

abstract = {To overcome reproductive dysfunctions, recombinant gonadotropin hormones have been synthesised in different fish species. We were interested in gaining further insight into the effects of recombinant follicle-stimulating hormone (rFsh) in meagre Argyrosomus regius spermatogenesis and in improving the existing knowledge on the role of testicular apoptosis. Germ cell composition and testicular apoptosis were compared in pre-pubertal fish treated with rFsh (PreP-Fsh) and controls (PreP-C), and adult meagre with testes in the proliferative (Adult-Pro) and meiotic (Adult-Meio) spermatogenesis phases. A lower density of committed spermatogonia was observed in PreP-Fsh compared with PreP-C and in Adult-Meio compared with Adult-Pro. In pre-pubertal fish and in Adult-Pro, apoptosis affected mainly individual germ cells and Sertoli cells. In Adult-Meio, a high number of cysts containing apoptotic germ cells was observed, and apoptosis often involved entire clones of germ cells. rFsh reduced testicular apoptosis in pre-pubertal fish, whereas the advancement of spermatogenesis in adults was associated with an increased density of apoptotic cells. The present study corroborated previous observations on the effects of rFsh on spermatogenesis in pre-pubertal meagre and provided further insights on the changing role of apoptosis in the pre-pubertal phase versus different phases of spermatogenesis.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{racaku_complex_2025,

title = {Complex Sex Determination in the Grey Mullet Mugil cephalus Suggested by Individual Whole Genome Sequence Data},

author = {Mbarsid Racaku and Serena Ferraresso and Massimiliano Babbucci and Andres Blanco and Costas S Tsigenopoulos and Tereza Manousaki and Jelena Radojicic and Vasileios Papadogiannis and Paulino Martínez and Luca Bargelloni and Tomaso Patarnello},

url = {https://www.mdpi.com/2076-2615/15/16/2445

/wp-content/uploads/2025/09/2025-Racaku-ANIMALS-52.pdf},

doi = {10.3390/ani15162445},

issn = {2076-2615},

year  = {2025},

date = {2025-09-25},

urldate = {2025-09-25},

journal = {Animals},

volume = {15},

number = {16},

pages = {2445},

abstract = {Mugil cephalus is a cosmopolitan marine fish highly relevant from ecological and economic perspectives. Previous studies identified sex-associated variants in the follicle-stimulating hormone receptor (fshr) gene following an XX/XY sex determination (SD) system. However, these variants could not be fully associated with sex in all samples. This suggests other genes and/or environmental factors may be involved in the SD of this species, denoting intraspecific variation. In this study, we constructed a new high-quality genome assembly of M. cephalus. We then re-sequenced the whole genome in males and females from two divergent Mediterranean populations to ascertain whether other genetic variants could also be involved in SD. fshr gene variants showed to only partially explain SD, while a new intronic variant in the sestd1 gene appeared to be associated with SD following a ZZ/ZW system. The presence of other putative candidate SD variants showing significant differences between the two populations suggested a regional pattern of variation in SD in the Mediterranean Sea. The incomplete association of all the identified variants also pointed to a potential role for environmental factors.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{metcalfe_submarine_2025,

title = {Submarine ash megabed fed by far-traveled, shoreline-crossing pyroclastic currents from a large explosive volcanic eruption},

author = {Abigail Metcalfe and Tim Druitt and Katharina Pank and Steffen Kutterolf and Jonas Preine and Karim Kelfoun and Christian Hübscher and Paraskevi Nomikou and Thomas A Ronge and Shun Chiyonobu and Olga Koukousioura and Adam Woodhouse and Sarah Beethe and Michael Manga and Iona McIntosh and Masako Tominaga and Carole Berthod and Hehe Chen and Acacia Clark and Susan DeBari and Ralf Gertisser and Raymond Johnston and Ally Peccia and Yuzuru Yamamoto and Alexis Bernard and Tatiana Fernandez Perez and Christopher K Jones and Kumar Batuk Joshi and Günther Kletetschka and Molly McCanta and Antony Morris and Paraskevi Polymenakou and Xiaohui Li and Jean-Marie Nedelec and Hao-Yang Lee and Dimitrios Papanikolaou},

url = {https://www.science.org/doi/10.1126/sciadv.ads9642

/wp-content/uploads/2025/09/2025-Metcalfe-Sci-Adv-51.pdf},

doi = {10.1126/sciadv.ads9642},

issn = {2375-2548},

year  = {2025},

date = {2025-09-22},

urldate = {2025-09-22},

journal = {Science Advances},

volume = {11},

number = {33},

pages = {eads9642},

abstract = {Large explosive volcanic eruptions from island volcanoes deliver vast quantities of ash to the marine environment. While many of the transport pathways are understood, those from shoreline-crossing or submarine pyroclastic currents, and their transformation into water-supported gravity flows, remain poorly constrained. We report the discovery by International Ocean Discovery Program (IODP) deep drilling of a 200-meter-thick ash megabed buried in rift basins of the South Aegean Volcanic Arc. The textgreater73–cubic kilometer ash deposit originates from the Kos Plateau Tuff caldera-forming eruption, which occurred 161 thousand years ago, textgreater120 kilometers to the east. The ash forms a chemically uniform, graded megabed lacking bioturbation, interpreted as having been emplaced by a stream of eruption-fed turbidity currents. Bioclastic debris within the ash provides evidence of widespread destruction of marine ecosystems. Large volcanic eruptions can remodel the seafloor landscape, deposit thick ash turbidites, and destroy marine biota on island arc–wide scales in short-lived, catastrophic events. 

 , 

 Volcanic ash from an eruption in Greece poured into the sea, forming a bed 200 m thick in a submarine basin far from the volcano.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{gittings_remotely_2025,

title = {Remotely Sensing Phytoplankton Size Structure in the Mediterranean Sea: Insights from In Situ Data and Temperature-Corrected Abundance-Based Models},

author = {John A Gittings and Eleni Livanou and Xuerong Sun and Robert J W Brewin and Stella Psarra and Manolis Mandalakis and Alexandra Peltekis and Annalisa Di Cicco and Vittorio E Brando and Dionysios E Raitsos},

url = {https://www.mdpi.com/2072-4292/17/14/2362

/wp-content/uploads/2025/09/2025-Gittings-RemSense-50.pdf},

doi = {10.3390/rs17142362},

issn = {2072-4292},

year  = {2025},

date = {2025-09-15},

urldate = {2025-09-16},

journal = {Remote Sensing},

volume = {17},

number = {14},

pages = {2362},

abstract = {Since the mid-1980s, the Mediterranean Sea’s surface and deeper layers have warmed at unprecedented rates, with recent projections identifying it as one of the regions most impacted by rising global temperatures. Metrics that characterize phytoplankton abundance, phenology and size structure are widely utilized as ecological indicators that enable a quantitative assessment of the status of marine ecosystems in response to environmental change. Here, using an extensive, updated in situ pigment dataset collated from numerous past research campaigns across the Mediterranean Sea, we re-parameterized an abundance-based phytoplankton size class model that infers Chl-a concentration in three phytoplankton size classes: pico- (textless2 μm), nano- (2–20 μm) and micro-phytoplankton (textgreater20 μm). Following recent advancements made within this category of size class models, we also incorporated information of sea surface temperature (SST) into the model parameterization. By tying model parameters to SST, the performance of the re-parameterized model was improved based on comparisons with concurrent, independent in situ measurements. Similarly, the application of the model to remotely sensed ocean color observations revealed strong agreement between satellite-derived estimates of phytoplankton size structure and in situ observations, with a performance comparable to the current regional operational datasets on size structure. The proposed conceptual regional model, parameterized with the most extended in situ pigment dataset available to date for the area, serves as a suitable foundation for long-term (1997–present) analyses on phytoplankton size structure and ecological indicators (i.e., phenology), ultimately linking higher trophic level responses to a changing Mediterranean Sea.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{digenis_assessing_2025,

title = {Assessing the Motile Fauna of Eastern Mediterranean Marine Caves},

author = {Markos Digenis and Michail Ragkousis and Charalampos Dimitriadis and Stelios Katsanevakis and Vasilis Gerovasileiou},

url = {https://www.mdpi.com/2410-3888/10/8/383

/wp-content/uploads/2025/09/2025-Digenis-fishes-49.pdf},

doi = {10.3390/fishes10080383},

issn = {2410-3888},

year  = {2025},

date = {2025-09-12},

urldate = {2025-09-15},

journal = {Fishes},

volume = {10},

number = {8},

pages = {383},

abstract = {Although marine caves are among the most species-diverse habitats in the Mediterranean Sea, most available studies have focused on their sessile fauna. This study provides the first quantitative assessment of motile fauna in 27 marine caves across four geographical subareas of the Aegean and Ionian Seas, using a rapid assessment visual census protocol, applied through 3 min time transects in each ecological cave zone. Multivariate analysis revealed that the motile community structure of the cave entrance was differentiated from that of the semidark and dark zones. Deeper caves were distinct from shallower ones while caves of the east Aegean differed from those around Crete Island. A total of 163 taxa were recorded, 27 of which are reported herein for the first time in marine caves of the eastern Mediterranean Sea, while three species (two native and one introduced) are recorded in Greek waters for the first time, enriching our knowledge on the permanent and occasional cave residents. Seventeen species were introduced, comprising more than half of the total fish abundance in the southeasternmost cave. Our limited knowledge of the motile fauna of Mediterranean marine caves coupled with the continued spread of introduced species highlights the urgent need for monitoring and conservation actions, especially within marine protected areas.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{tampou_growth_2025,

title = {Growth Performance of Gilthead Sea Bream ( \textit{Sparus aurata} ) Fed Low Fish Meal Diets With an Innovative Mixture of Low Trophic Ingredients},

author = {Anna Tampou and Katerina Kousoulaki and Antigoni Vasilaki and Nikolaos Vlahos and Eleni Nikouli and Nikolas Panteli and Konstantinos Feidantsis and Konstantinos Kormas and Styliani Andreopoulou and Ioannis T Karapanagiotidis and Panagiotis Berillis and Ioannis Nengas and Efthimia Antonopoulou and Eleni Mente},

editor = {Noah Esmaeili},

url = {https://onlinelibrary.wiley.com/doi/10.1155/anu/7504207

/wp-content/uploads/2025/09/2025-Tampou-Aqua-Nutr-48.pdf},

doi = {10.1155/anu/7504207},

issn = {1353-5773, 1365-2095},

year  = {2025},

date = {2025-09-11},

urldate = {2025-09-12},

journal = {Aquaculture Nutrition},

volume = {2025},

number = {1},

pages = {7504207},

abstract = {This study examined the growth performance, cellular signaling, and gut microbiome of gilthead sea bream ( 

 Sparus aurata 

 ) fed four diets using low‐trophic‐level ingredients for 65 days. Control (C) diet contained fish meal (FM) as the main protein source and fish oil (FO) as a lipid source. In the 0%FMFO diet all FM and FO present in the C diet was replaced with a combination of microalgae, insect meal (IM), and tunicate meal (TM). IM and TM diets were formulated to contain 20% of the novel protein ingredients, replacing 68.09% and 45.91% of FM in diet C, respectively. Compared to diet C, feed utilization and growth performance of 

 S 

 . 

 aurata 

 fed 0%FMFO diet was not different ( 

 p 

 textgreater 0.05) and TM diet had a significantly lower ( 

 p 

   textless 0.05) specific growth rate (SGR), higher ( 

 p 

   textless 0.05) feed conversion ratio (FCR) and decreased fillet lipid content ( 

 p 

 textless 0.05). In liver and foregut of fish fed TM diet the activity of lactate dehydrogenase (L‐LDH) significant increased ( 

 p 

 textless 0.05), indicating higher glycolytic potential, whereas the decrease in Hsp70, Hsp90, AMPK, and p38 MAPK may indicate reduced stress response. Fish midgut microbiome included beneficial taxa for the host. The results suggested that the mixture of algae, insect, and TM could replace all FM and FO in gilthead sea bream diets without affecting the fish growth performance.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{christou_climate_2025,

title = {Climate change in the “vulnerable” Eastern Mediterranean and adjacent areas: A literature review of ecological impacts and threats},

author = {Epaminondas D Christou and Thanos Dailianis and Evangelia Chatzinikolaou and Paraskevi K Karachle and Dimitrios Damalas and Konstantinos Tsagarakis and Evangelia Strogyloudi and Georgios Karris and Soultana Zervoudaki and Antonia Giannakourou and Maria Salomidi and Sofia Reizopoulou},

url = {https://linkinghub.elsevier.com/retrieve/pii/S0141113625004477

/wp-content/uploads/2025/09/2025-Christou-MarEnvRes-47.pdf},

doi = {10.1016/j.marenvres.2025.107390},

issn = {01411136},

year  = {2025},

date = {2025-09-08},

urldate = {2025-09-09},

journal = {Marine Environmental Research},

volume = {211},

pages = {107390},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{holm_first_2025,

title = {First island-wide, single-day soil collection study on Crete reveals environmental drivers of microbial diversity},

author = {Johanna B Holm and Savvas Paragkamian and Mike Humphreys and Apaala Chatterjee and Stephanie Yarwood and Josh Gaimaro and Melanthia Stavroulaki and Dimitris Tsaparis and Manolis Plaitis and Panagiotis Kasapidis and Stelios Darivianakis and Georgios Kotoulas and Antonios Magoulas and Anastasis Oulas and Zacharias Kyrpiotakis and Pavlos Pavlidis and Petra Ten Hoopen and Guy Cochrane and Wiebe H C F Kooistra and Jason R Schriml and Bronwen E Schriml and Ilias Lagkouvardos and Panos Gkorezis and Neil Davies and Christine Laney and Lee Stanish and Granger Sutton and Scott Tighe and Ilene Karsch Mizrachi and Donovan Parks and Pelin Yilmaz and Chelsea Carey and Pier Buttigieg and Philip Goldstein and Evangelos Pafilis and Lynn M Schriml},

url = {https://environmentalmicrobiome.biomedcentral.com/articles/10.1186/s40793-025-00752-z

/wp-content/uploads/2025/09/2025-Holm-Paragkamian-EnvMicro-46.pdf},

doi = {10.1186/s40793-025-00752-z},

issn = {2524-6372},

year  = {2025},

date = {2025-09-04},

urldate = {2025-09-08},

journal = {Environmental Microbiome},

volume = {20},

number = {1},

pages = {94},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{rotter_innovative_2025,

title = {Innovative solutions for valorization of desalination brine},

author = {Ana Rotter and Baruch Rinkevich and Irem Deniz and Maggie M Reddy and Mariana Girão and Maria F Carvalho and Nina Gunde-Cimerman and Cene Gostinčar and Mercedes Cueto and Ana R Díaz-Marrero and Viktoriia Komarysta and Fusun Akgul and Lada Lukić Bilela and Ernesta Grigalionyte-Bembič and Manolis Mandalakis},

url = {https://linkinghub.elsevier.com/retrieve/pii/S2589914725000714

/wp-content/uploads/2025/09/2025-Rotter-WaterRX-45.pdf},

doi = {10.1016/j.wroa.2025.100372},

issn = {25899147},

year  = {2025},

date = {2025-09-01},

urldate = {2025-09-05},

journal = {Water Research X},

volume = {28},

pages = {100372},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{stavrakidis-zachou_nutritional_2025,

title = {A Nutritional Bioenergetic Model for Farmed Fish: Effects of Food Composition on Growth, Oxygen Consumption and Waste Production},

author = {Orestis Stavrakidis-Zachou and Ep H Eding and Nikos Papandroulakis and Konstadia Lika},

editor = {Liqiao Chen},

url = {https://onlinelibrary.wiley.com/doi/10.1155/anu/9010939

/wp-content/uploads/2025/09/2025-Stavrakidis-Zachou-AquaNutr-44.pdf},

doi = {10.1155/anu/9010939},

issn = {1353-5773, 1365-2095},

year  = {2025},

date = {2025-08-29},

urldate = {2025-09-04},

journal = {Aquaculture Nutrition},

volume = {2025},

number = {1},

pages = {9010939},

abstract = {The study of flow and transformation of energy and nutrients via mathematical modelling provides an in silico tool approach for designing scientific experiments, improving precision in aquaculture production and reducing the need for experimental animals. The proposed nutritional bioenergetics model is based on the dynamic energy budget (DEB) theory, a mechanistic framework to study individual metabolism. The model is an extension of the typical DEB models in that it includes a digestion module where the protein and non‐protein food components contribute to assimilation via the concept of a synthesising unit (SU). The model allows predictions for measurable quantities of interest for aquaculture, including feeding rate, oxygen consumption, carbon dioxide, ammonia and solid waste production, under various temperatures and feeding conditions, both in terms of quantity and macronutrient composition. The feeding schedule’s effects, such as the diurnal variation in waste production in response to feeding frequency, are also captured. The model quantifies the effects of the dietary protein‐to‐energy ratio on food intake and assimilation; energy‐rich diets or those with excessive or poor amounts of protein show reduced intake. The model has been parametrised and validated for rainbow trout ( 

 Oncorhynchus mykiss 

 ) to demonstrate its capabilities. Testing the model with diverse datasets has shown that it predicts weight gain well, and to a lesser extent, oxygen consumption and total ammonia production. The proposed model could be a useful in silico tool for fish researchers, technicians and farm operators.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{conides_innovative_2025,

title = {An Innovative Metal–Synthetic Hybrid Thread for the Construction of Aquaculture Nets},

author = {Alexis Conides and Efthimia Cotou and Dimitris Klaoudatos and Branko Glamuzina},

url = {https://www.mdpi.com/2077-1312/13/8/1384

/wp-content/uploads/2025/09/2025-Conides-MSciEng-43.pdf},

doi = {10.3390/jmse13081384},

issn = {2077-1312},

year  = {2025},

date = {2025-08-27},

urldate = {2025-09-03},

journal = {Journal of Marine Science and Engineering},

volume = {13},

number = {8},

pages = {1384},

abstract = {Based on the experience gained worldwide from potential solutions to the fouling problem of fisheries and aquaculture infrastructure, we attempted to design, construct and test the antifouling efficiency of a new hybrid filament created from non-laminated copper wire braided with synthetic fibers made of Dyneema. The design involved the creation of a hybrid twine substituting a percentage of the synthetic fibers with 0.1–0.15 mm diameter copper wire at 5%, 10%, 20% and 40% levels. There is limited information in the international literature for comparison with our results, since there has never been any attempt to create such a hybrid net. The results showed that for the 6 mm mesh, the maximum openness obtained after the 8-month experimental period was 8.72%, with Cu wire substitution at 35%. For the 12 mm mesh, these values were 27.07% at 26%, and for the 20 mm mesh, they were 33.68% at 28%. A conservative average independent from mesh size to achieve optimum openness in the long term is 30 ± 4.73% Cu wire substitution. In addition, we found that both the mesh size (mm) and the copper substitution percentage affected the fouling process during the experimental period, which lasted 8 months.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{salomidi_advancing_2025,

title = {Advancing knowledge on red coral Corallium rubrum (Linnaeus, 1758) populations and associated mesophotic communities in the Aegean Sea, Eastern Mediterranean},

author = {Maria Salomidi and Alfredo Marchiò and Yiannis Issaris and Vasilis Gerakaris and Thanos Dailianis and Vasilis Gerovasileiou},

url = {https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/41403

/wp-content/uploads/2025/09/2025-Salomidi-MMS-42.pdf

},

doi = {10.12681/mms.41403},

issn = {1791-6763, 1108-393X},

year  = {2025},

date = {2025-08-25},

urldate = {2025-09-02},

journal = {Mediterranean Marine Science},

volume = {26},

number = {2},

pages = {378--392},

abstract = {This study presents the first quantitative analysis of live red coral (Corallium rubrum) populations in Greek waters (North-Western Aegean Sea) and contributes to the limited knowledge of mesophotic benthic assemblages in the Eastern Mediterranean Sea. Utilizing ROV and stereo-video SCUBA surveys at two previously harvested sites (47-60 m depth), we quantified red coral density and morphometric parameters. Associated benthic communities, comprising 101 taxa with Porifera as the dominant group, were characterized, and the Mesophotic Assemblages Conservation Status (MACS) index was applied for the first time in this region, revealing an overall “Good” ecological status despite locally degraded facies. While red coral entanglement by widespread ghost fishing gear appeared low, high frequencies of necrosis and epibiosis were observed in the larger canopy-forming gorgonian Paramuricea clavata, potentially indicating current and past mechanical damage, although the synergistic effects of other physical stressors cannot be excluded. This research offers crucial baseline data on red coral populations and associated mesophotic communities in an under-studied region and ecological zone. Our findings are discussed in the context of existing national management frameworks and conservation measures, emphasizing the need for further monitoring and research to inform effective management and conservation strategies in view of increasing anthropogenic and environmental pressures.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{angelakopoulos_effects_2025,

title = {The Effects of Early Temperature and Live Feeds on the Development of White Muscle in Greater Amberjack (Seriola dumerili)},

author = {Rafael Angelakopoulos and Andreas Tsipourlianos and Alexia E Fytsili and Nikolaos Mitrizakis and Themistoklis Giannoulis and Nikos Papandroulakis and Katerina A Moutou},

url = {https://www.mdpi.com/2410-3888/10/7/360

/wp-content/uploads/2025/08/2025-Angelakopoulos-Fishes-41.pdf},

doi = {10.3390/fishes10070360},

issn = {2410-3888},

year  = {2025},

date = {2025-08-11},

urldate = {2025-08-11},

journal = {Fishes},

volume = {10},

number = {7},

pages = {360},

abstract = {Greater amberjack (Seriola dumerili) shows potential for Mediterranean aquaculture due to its swift growth, consumer appeal, and commercial value. However, challenges in juvenile production, such as growth dispersion and unsynchronized development, impede further expansion. This study explores the impact of rearing temperature and live feed types on early white muscle development in greater amberjack larvae. Findings reveal substantial effects of temperature and diet on larval development, highlighting that the combination of 24 °C and a copepod + rotifer co-feeding scheme resulted in the highest axial growth rate, whereas rotifer-fed larvae at 20 °C exhibited a slower pace. Incorporating both histological and gene expression analyses, the study underscores temperature’s significant influence on white muscle development. Among larvae reared at 24 °C, the two live feed types led to phenotypic variations at metamorphosis, with rotifers supporting longer larvae featuring a smaller total cross-sectional area compared to copepods. Gene expression analysis indicates heightened mylpfb and myog expression at 24 °C during early larval stages, suggesting increased hyperplasia and myoblast differentiation. This study highlights the necessity of considering both temperature and feed type in larval rearing practices for optimal muscle development, and further research exploring combined diets during rearing could offer insights to enhance amberjack aquaculture sustainability.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{idan_testing_2025b,

title = {Testing the effect of host availability on endobiont diversity: proposing the single hotel hypothesis},

author = {Tal Idan and Sigal Shefer and Giorgos Chatzigeorgiou and Vasilis Gerovasileiou and Liron Goren},

url = {https://www.nature.com/articles/s41598-025-09496-9

/wp-content/uploads/2025/08/2025-Idan-SciReport-40.pdf},

doi = {10.1038/s41598-025-09496-9},

issn = {2045-2322},

year  = {2025},

date = {2025-08-01},

urldate = {2025-08-01},

journal = {Scientific Reports},

volume = {15},

number = {1},

pages = {21717},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{wylie_effects_2025,

title = {Effects of hormonal treatments on spawning latency and egg production of captive silver trevally (\textit{Pseudocaranx georgianus})},

author = {Matthew J Wylie and Flavio F Ribeiro and Warren Fantham and Morgan E Puklowski and Ria Rebstock and Nicola Shaw and Constantinos C Mylonas and Keitaro Kato and Abigail Elizur and Maren Wellenreuther},

url = {https://linkinghub.elsevier.com/retrieve/pii/S2352513425003503

/wp-content/uploads/2025/07/2025-Wylie-AquaReports-39.pdf},

doi = {10.1016/j.aqrep.2025.102964},

issn = {23525134},

year  = {2025},

date = {2025-07-30},

urldate = {2025-07-31},

journal = {Aquaculture Reports},

volume = {43},

pages = {102964},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{moller_comparative_2025,

title = {Comparative assessment of pelagic/benthic microalgae in the oligotrophic continental shelf of Heraklion Bay, Eastern Mediterranean: A pigments-based chemotaxonomic analysis},

author = {Ben Möller and Eleni Livanou and Stella Psarra and Panayota Koulouri and Costas Dounas and Ana Rotter and Manolis Mandalakis},

url = {https://linkinghub.elsevier.com/retrieve/pii/S2352485525003111

/wp-content/uploads/2025/07/2025-Moller-RegStMB-38.pdf},

doi = {10.1016/j.rsma.2025.104320},

issn = {23524855},

year  = {2025},

date = {2025-07-24},

urldate = {2025-07-25},

journal = {Regional Studies in Marine Science},

volume = {89},

pages = {104320},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{kotsiri_seasonal_2025,

title = {Seasonal Variation in Nutritional Value and Technical Quality of Lionfish (Pterois miles) from the Ionian and Aegean Seas},

author = {Mado Kotsiri and Dimitra Kogiannou and Chrisanthi Nikoloudaki and Ioannis Kleidas and Aikaterini Dogrammatzi and Paraskevi K Karachle and Kriton Grigorakis},

url = {https://www.mdpi.com/2304-8158/14/13/2353

/wp-content/uploads/2025/07/2025-Kotsiri-foods-37.pdf

},

doi = {10.3390/foods14132353},

issn = {2304-8158},

year  = {2025},

date = {2025-07-18},

urldate = {2025-07-18},

journal = {Foods},

volume = {14},

number = {13},

pages = {2353},

abstract = {Lionfish (Pterois miles), an invasive species in the Mediterranean, pose ecological and socioeconomic challenges. This study examines the seasonal variation in the nutritional composition and technical quality of lionfish from the Ionian and Aegean Seas, evaluating their potential as a food resource. Fillets were high in protein (19.4%) and low in fat (2.0%), with significant seasonal differences in the Ionian Sea, where winter samples had higher lipid content. The fillet yield (28.4%) was satisfactory given the fish’s morphology. Fatty acid analysis confirmed lionfish as a valuable source of polyunsaturated fatty acids (PUFAs), particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), with EPA + DHA levels exceeding the recommended daily intake (119.2%). Seasonal variations in fatty acid composition were observed, including higher EPA in autumn and lower lipid nutritional quality in summer. Arachidonic acid (ARA) was also present at nutritionally significant levels (79.4 mg/100 g). The n3/n6 ratio (2.2) and favourable atherogenic and thrombogenic indices highlight its nutritional benefits. This is the first study to assess seasonal variations in the nutritional value and technical yield of lionfish in the Mediterranean, offering novel insights into its commercial valorisation. These findings support its promotion as a sustainable protein source and as a means of managing its invasive population.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{manga_low_2025,

title = {Low Heat Flow in the Anhydros Basin, Aegean Sea, Recorded by Deep Subsurface Temperatures},

author = {Michael Manga and Masako Tominaga and Jonas Preine and Thomas A Ronge and Sarah Beethe and Christian Hübscher and Iona McIntosh and Paraskevi Nomikou and Steffen Kutterolf and Tim Druitt and Alexis Bernard and Carole Berthod and Hehe Chen and Acacia Clark and Susan DeBari and Tatiana I Fernandez‐Perez and Ralf Gertisser and Raymond M Johnston and Christopher K Jones and Batuk K Joshi and Günther Kletetschka and Olga Koukousioura and Molly McCanta and Antony Morris and Katharina Pank and Ally Peccia and Paraskevi N Polymenakou and Adam Woodhouse and Yuzuru Yamamoto},

url = {https://agupubs.onlinelibrary.wiley.com/doi/10.1029/2025GL115919

/wp-content/uploads/2025/07/2025-Manga-GRL-36.pdf},

doi = {10.1029/2025GL115919},

issn = {0094-8276, 1944-8007},

year  = {2025},

date = {2025-07-16},

urldate = {2025-07-16},

journal = {Geophysical Research Letters},

volume = {52},

number = {13},

pages = {e2025GL115919},

abstract = {Abstract 

 

 Subseafloor in situ temperatures in a drilled hole in the Anhydros Basin, Aegean Sea, measured during International Ocean Discovery Program (IODP) Expedition 398, yielded a low heat flow (textless0.023 W/m 

 2 

 ) despite active magmatism and rifting in the region. The coldest and highest temperatures were 13.9°C at 52.5 m below seafloor (mbsf) and 15.5°C for the deepest measurement at 360.4 mbsf, respectively. Comparison of a heat transfer model with measurements suggests that sea bottom temperatures during the last glacial period were up to 10°C cooler than Holocene temperatures. The magnitude of Holocene warming co‐varies with the geothermal heat flow: if the former goes up the latter goes up. Low heat flow may arise from lateral removal of heat through deeper formations by gravity driven advection of fluids. Tectonic separation of the northwestern Anhydros Basin from the Christiana‐Santorini‐Kolumbo volcanic field may lead to minimal magmatic influences on heat flow. 

 

 , 

 Plain Language Summary 

 Temperatures in Earth's crust reveal the processes that create and transport heat. In the rifting Anhydros Basin in the Aegean Sea, north of the active South Aegean Volcanic Arc, International Ocean Discovery Program Expedition 398 measured cold subsurface temperatures in a borehole to depths exceeding 300 m below the seafloor. These temperatures record low heat flow and cold seafloor temperatures from the last glacial period. The low heat flow at shallow depths may be due to deeper fluid circulation that removes heat. Low heat flow further implies that there are no magma bodies within the crust in the northwestern part of the Anhydros Basin. 

 , 

 Key Points 

 

 

 

 Heat flow is low in the Anhydros Basin, Aegean Sea 

 

 

 Ocean bottom temperatures were cold during the last glacial 

 

 

 Subsurface temperature records surface temperature changes and heat flow},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{dritsas_investigating_2025,

title = {Investigating the Potential of Newly Isolated Microalgae Strains from the Ionian Sea (Greece) Cultured in an Open Raceway Pond},

author = {Panagiotis Dritsas and Stefania Patsialou and Dimitrios Kampantais and Efstratios Roussos and Yannis Kotzamanis and Athanasia Tekerlekopoulou and Dimitris V Vayenas and George Aggelis},

url = {https://www.mdpi.com/2076-3417/15/12/6680

/wp-content/uploads/2025/07/2025-Dritsas-AppSci-35.pdf},

doi = {10.3390/app15126680},

issn = {2076-3417},

year  = {2025},

date = {2025-07-07},

urldate = {2025-07-10},

journal = {Applied Sciences},

volume = {15},

number = {12},

pages = {6680},

abstract = {Microalgae are a subject of interest not only for fundamental research but for various biotechnological applications as well. In this study, the ability of newly isolated strains, i.e., Picochlorum costavermella VAS2.5, Picochlorum oklahomense SAG4.4, Microchloropsis gaditana VON5.3, and Nephroselmis pyriformis PAT2.7, to grow when cultured in an open raceway pond under laboratory conditions and produce various metabolites of high-added value was evaluated. N. pyriformis PAT2.7 and P. costavermella VAS2.5 were the greatest in biomass production (exceeding 0.4 g/L), while P. costavermella VAS2.5 and M. gaditana VON5.3 were the greatest in lipid production (reaching approximately 18%, wt/wt). The lipid fraction of glycolipids and sphingolipids was predominant (43.6–55.4%, wt/wt), followed by neutral lipids (27.1–50.1%, wt/wt) and phospholipids (6.9–17.4%, wt/wt). Picochlora and M. gaditana VON5.3 lipids were rich in Δ5,8,11,14,17C20:5 and/or Δ9,12,15C18:3, while N. pyriformis PAT2.7 synthesized Δ9C16:1 in large quantities (30–40%, wt/wt). All strains showed remarkable yields in polysaccharide and protein production, demonstrated a well-balanced amino acid profile, and synthesized pigments in amounts comparable to other studies. The biochemical profiles of these strains showcased their suitability for use primarily in the aquaculture sector.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{vasilaki_nutritional_2025,

title = {Nutritional enhancement of lupin meal (\textit{Lupinus albus}), through fermentation with textitSaccharomyces cerevisiae, as plant protein ingredient in aquafeeds for the European sea bass (\textit{Dicentrarchus labrax}).},

author = {Antigoni Vasilaki and Ioannis Nengas and Eleni Fountoulaki and Morgane Henry and Dimitra Kogiannou and Chrysanthi Nikoloudaki and Petros Chronopoulos and Ioannis T Karapanagiotidis and Elena Mente},

url = {https://www.sciencedirect.com/science/article/pii/S0044848625007069

/wp-content/uploads/2025/07/2025-Vasilaki-AQUA-34.pdf},

doi = {10.1016/j.aquaculture.2025.742820},

issn = {0044-8486},

year  = {2025},

date = {2025-07-04},

urldate = {2025-07-09},

journal = {Aquaculture},

volume = {609},

pages = {742820},

abstract = {Legumes contain antinutritional factors that restrict their use in fish nutrition, however, biotechnological methods such as solid-state fermentation can improve their nutritional profile, positioning them as a sustainable alternative for aquafeed formulation. This research evaluated the feasibility of replacing soybean meal with Saccharomyces cerevisiae-fermented lupin meal (Lupinus albus) in feed formulations for European sea bass (Dicentrarchus labrax). Four diets were produced; one commercial type diet (FRL0) and three diets with partial (FRL10, FRL12.5) or complete (FRL15) replacement of soybean meal by fermented lupin meal. European sea bass juveniles, with an initial average weight: 18.9 g, were fed the experimental diets for 71 days in triplicate groups. Results indicated no adverse effects on key production indices when soybean meal was replaced partially or totally. Fermented lupin meal linearly improved feed utilization. Apparent digestibility coefficient of protein significantly increased in groups fed 12.5 % and 15 % fermented lupin, as did apparent digestibility coefficient of fat, compared to the FRL0 group. Trypsin activity in pyloric caeca was statistically elevated in the fermented lupin groups compare to FRL0 group. Whole-body composition, amino acid content, and deposition showed no significant differences among groups. Fermented lupin did not suppress the immune system, and a slight immunostimulatory effect was observed at a 12.5 % inclusion level. No histopathological alterations were detected. Overall, the results demonstrated the efficacy of solid-state fermentation process in enhancing lupin meal, highlighting its potential as a sustainable replacement for soybean meal in feed formulations for Mediterranean marine aquaculture species such us D. labrax.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{droubogiannis_genome_2025,

title = {Genome sequences of textitVibrio harveyi VH2 and textitVibrio harveyi Vhp1-sp},

author = {Stavros Droubogiannis and Andriana Triga and Michail Pavlidis and Pantelis Katharios},

editor = {Frank J Stewart},

url = {https://journals.asm.org/doi/10.1128/mra.00087-25

/wp-content/uploads/2025/07/2025-Droubogiannis-MIcro-RA-33.pdf},

doi = {10.1128/mra.00087-25},

issn = {2576-098X},

year  = {2025},

date = {2025-06-30},

urldate = {2025-07-01},

journal = {Microbiology Resource Announcements},

pages = {e00087--25},

abstract = {ABSTRACT 

 

 

 

 Vibrio harveyi 

 is a major bacterial pathogen of marine aquatic animals causing significant economic losses in aquaculture. Here, we present the complete genomes of two pathogenic strains, VH2 (5.84 Mb, 44.99% GC) and Vhp1-sp (6.30 Mb, 44.98% GC), which encode numerous virulence factors, resistance genes, and genomic islands.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{lancerotto_administration_2025,

title = {Administration of single-chain recombinant follicle-stimulating hormone (sdrFsh) and luteinizing hormone (sdrLh) stimulates spermatogenesis, but not vitellogenesis in pre-pubertal greater amberjack (\textit{Seriola dumerili})},

author = {Stefano Lancerotto and Ioannis Fakriadis and Maria Papadaki and Irini Sigelaki and Ignacio Giménez and Jose Vicente Roig Genoves and Andrea Meloni and Constantinos C Mylonas},

url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848625006817

/wp-content/uploads/2025/06/2025-Lancerotto-AQUA-pre-print32.pdf},

doi = {10.1016/j.aquaculture.2025.742795},

issn = {00448486},

year  = {2025},

date = {2025-06-24},

urldate = {2025-06-24},

journal = {Aquaculture},

volume = {609},

pages = {742795},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{keklikoglou_disrupted_2025,

title = {Disrupted darkness: the impact of anthropogenic light at night on melatonin secretion of \textit{Hermodice carunculata (Polychaeta, Annelida)}},

author = {Kleoniki Keklikoglou and Manolis Mandalakis and Eleftheria Fanouraki and Thekla I Anastasiou and Eirini Kagiampaki and Katerina Vasileiadou and Christina Pavloudi and Christos Arvanitidis and Michail Pavlidis},

url = {https://link.springer.com/10.1007/s43630-025-00744-5

/wp-content/uploads/2025/06/2025-Keklikoglou-PP-Sci-31.pdf},

doi = {10.1007/s43630-025-00744-5},

issn = {1474-905X, 1474-9092},

year  = {2025},

date = {2025-06-20},

urldate = {2025-06-20},

journal = {Photochemical & Photobiological Sciences},

volume = {24},

number = {6},

pages = {1031--1040},

abstract = {Abstract 

 

 Anthropogenic light at night (ALAN) can have serious impacts on marine environments. Several studies have demonstrated that ALAN disrupts melatonin production, a hormone critical for regulating circadian rhythm. In this study, the effects of ALAN on melatonin and two of its related indolamines were investigated in the annelid 

 Hermodice carunculata 

 . Specifically, melatonin, serotonin and tryptamine levels were measured every three hours over a 24 h period in the heads of the annelids maintained under constant light and a 12 h light/12 h dark photoperiod, representing control conditions. Melatonin concentration was quantified using an enzyme immunoassay, while serotonin and tryptamine were analyzed by liquid chromatography–tandem mass spectrometry. Melatonin levels in annelid heads remained relatively constant with a pronounced peak at 11:00. A similar pattern was observed under constant light, but the melatonin peak shifted to 14:00. However, serotonin and tryptamine did not exhibit any significant diurnal variations due to constant light exposure. These findings suggest that melatonin secretion in 

 H. carunculata 

 is sensitive to ALAN, whereas its related indolamines are potentially not. The disruption of 

 H. carunculata' 

 s melatonin secretion pattern may affect its night-time behavior and reproduction, highlighting the need for further studies to assess the ecological effects of ALAN on various marine invertebrates. 

 

 

 Graphical abstract},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{oikonomou_validation_2025,

title = {Validation of QTL associated with resistance to Lernanthropus kroyeri in European seabass},

author = {Stavroula Oikonomou and Domniki Manousi and Arkadios Dimitroglou and Zoi Kazlari and Dimitrios Loukovitis and Kantham Papanna and Konstantinos Tzokas and Nikos Katribouzas and Leonidas Papaharisis and Costas S Tsigenopoulos and Dimitrios Chatziplis},

url = {https://linkinghub.elsevier.com/retrieve/pii/S0044848625006490

/wp-content/uploads/2025/06/2025-Oikonomou-AQUA-30.pdf},

doi = {10.1016/j.aquaculture.2025.742763},

issn = {00448486},

year  = {2025},

date = {2025-06-18},

urldate = {2025-06-18},

journal = {Aquaculture},

volume = {608},

pages = {742763},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{rizzo_can_2025,

title = {Can Marine Animal Forests benefit from existing conservation measures? A systematic approach towards the identification of protected sessile benthic species in the Mediterranean Sea},

author = {Lucia Rizzo and Tomás Vega Fernández and Francesca Necci and Michaël Grelaud and Patrizia Ziveri and Vasilis Gerovasileiou and Sergio Rossi},

url = {https://ejournals.epublishing.ekt.gr/index.php/hcmr-med-mar-sc/article/view/38550

/wp-content/uploads/2025/06/2025-Rizzo-MMS-29.pdf},

doi = {10.12681/mms.38550},

issn = {1791-6763, 1108-393X},

year  = {2025},

date = {2025-06-17},

urldate = {2025-06-17},

journal = {Mediterranean Marine Science},

volume = {26},

number = {2},

pages = {327--340},

abstract = {Some marine benthic invertebrates increase the structural complexity of the seabed, thereby providing suitable habitats to several associated species, resulting in biodiversity hotspots. Marine Animal Forests (MAFs) encompass a set of the most important marine benthic habitats into which diversified sessile suspension feeders like sponges, corals, sea pens, tube worms, bivalves, bryozoans and ascidians occur. Such a mix of sessile species brings characteristic assemblages and supports important ecosystem functions. In the last decades, some species which form MAFs have been the object of international conventions, EU directives, and national policies aiming to address natural and human-induced disturbances. Effective conservation, monitoring, and restoration actions require summarizing the available information to include MAFs in conservation plans. In the present work, the main international policies for the protection of coastal and marine fauna were screened in order to provide a list of protected species which form Mediterranean MAFs. These international normative documents include the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES), the Convention on the Conservation of European Wildlife and Habitats (Bern Convention), the European Habitats Directive, and the Protocol concerning Specially Protected Areas and Biological Diversity in the Mediterranean (SPA/BD Protocol) of the Barcelona Convention. The analysis of the normative items revealed that 62 Mediterranean potential MAF forming species have been included in one or more legal annexes as protected species. These species belong to different phyla, including Porifera, Cnidaria, Bryozoa and Mollusca. A wider view was proposed by the International Union for Conservation of Nature (IUCN), setting the bases of a legal acknowledgement of the MAFs as one of the largest biomes on Earth. This study represents a first step to obtain a baseline of MAF legal protection framework in order to support the further implementation of management measures aimed at increasing the effective protection of MAFs. Reporting the status of the Mediterranean MAF species that should be considered in management plans and conservation measures will be crucial for policymakers, as well as for mitigating current and future impacts on these distinctive marine environments.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{kouvara_assessing_2025,

title = {Assessing marine litter and its ecological impact on the seafloor of Thermaikos Gulf (NE Mediterranean Sea, Greece): Insights from ROV and diver surveys},

author = {Konstantina Kouvara and Evi Lazou-Laskaridis and Peggy Xirotagarou and Dimitris Christodoulou and Xenophon Dimas and Maria Geraga and Ioannis Giovos and Anastasia Charitou and Vasilis Gerovasileiou and Francois Galgani and George Papatheodorou},

url = {https://linkinghub.elsevier.com/retrieve/pii/S0025326X25005843

/wp-content/uploads/2025/06/2025-Kouvara-MPB-nonOPEN-28.pdf},

doi = {10.1016/j.marpolbul.2025.118109},

issn = {0025326X},

year  = {2025},

date = {2025-06-16},

urldate = {2025-06-16},

journal = {Marine Pollution Bulletin},

volume = {217},

pages = {118109},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{asimaki_effects_2025,

title = {The Effects of Dietary Fishmeal Substitution by Full‐Fat and Defatted textitZophobas morio Larvae Meals on Juvenile Gilthead Seabream (\textit{Sparus aurata}): An Integrative Approach},

author = {Adamantia Asimaki and Pier Psofakis and Elli-Zafeiria Gkalogianni and Aikaterini-Maria Katouni and Panagiotis Berillis and Konstantinos A Kormas and Christos I Rumbos and Christos G Athanassiou and Antigoni Vasilaki and Eleni Fountoulaki and Morgane Henry and Eleni Mente and Enric Gisbert and Ioannis T Karapanagiotidis},

editor = {Lee Seong Wei},

url = {https://onlinelibrary.wiley.com/doi/10.1155/anu/8885509

/wp-content/uploads/2025/06/2025-Asimaki-Aq-Nutr-27.pdf},

doi = {10.1155/anu/8885509},

issn = {1353-5773, 1365-2095},

year  = {2025},

date = {2025-06-10},

urldate = {2025-06-10},

journal = {Aquaculture Nutrition},

volume = {2025},

number = {1},

pages = {8885509},

abstract = {This study evaluated the dietary fishmeal substitution by full‐fat (FF) and defatted (DF) 

 Zophobas morio 

 meals regarding growth, feed efficiency, proximate and fatty acid compositions, digestive enzymes activities, histology and midgut microbiota in gilthead seabream ( 

 Sparus aurata 

 ). Juveniles initially weighing 3.4 g were distributed to triplicate groups and fed at satiation six isonitrogenous (8.41%) and isocaloric (21 Mj/kg) diets for 100 days. An insect meal‐free diet was the control (CTRL), two diets contained a FF 

 Z. morio 

 meal at 49 g/kg (FF‐49) and 97 g/kg (FF‐97), and three diets contained a DF 

 Z. morio 

 meal at 58 g/kg (DF‐58), 116 g/kg (DF‐116) and 174 g/kg (DF‐174) at the expense of fishmeal. Neither the form nor the inclusion level of 

 Z. morio 

 meals affected the feed intake of fish denoting a similar acceptability to that of fishmeal. Fish survival, growth and feed efficiency were not impaired by all dietary inclusion levels of 

 Z. morio 

 meals. Proximate composition of fish was altered but without indicating a clear correlation with the form or inclusion level of 

 Z. morio 

 . Increasing inclusions of both forms of 

 Z. morio 

 meals tended to decrease 22:6n‐3, 20:5n‐3, 18:3n‐3 and 18:2n‐6 levels in fish tissues. All fish exhibited similar proteolytic enzyme activities, but the increasing inclusions of both insect meal forms led to gradual increases in the lipase and α‐amylase activities indicating a compensatory mechanism for lipid and carbohydrate digestion. The use of 

 Z. morio 

 meals led to some mild histomorphological changes in the intestine and liver that were more pronounced in fish fed the FF form at the highest inclusion level. Midgut bacterial communities of the groups were similar and dominated by potentially beneficial members of 

 Saccharimonadales 

 and 

 Rhodobacteraceae 

 , except FF‐97 fish that had high abundances of 

 Legionella 

 ‐ and 

 Pandoraea 

 ‐like bacteria. To conclude, 

 Z. morio 

 meal, either FF or DF, is a suitable insect protein for fishmeal substitution towards more sustainable aquafeeds for 

 S. aurata 

 .},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{okeleye_common_2025,

title = {Common carp (\textit{Cyprinus carpio}) blood cells are modulated by a parasitic antimicrobial peptide, A-2S and Edwardsiella spp. strains},

author = {Oluwabusayo Israel Okeleye and Jovana Majstorovic and Anupa Sudharaka and Adriana Triga and Manolis Mandalakis and Pantelis Katharios and Ivona Mladineo},

url = {https://linkinghub.elsevier.com/retrieve/pii/S105046482500316X

/wp-content/uploads/2025/06/2025-Busayo-FSI-26.pdf},

doi = {10.1016/j.fsi.2025.110427},

issn = {10504648},

year  = {2025},

date = {2025-06-06},

urldate = {2025-06-06},

journal = {Fish & Shellfish Immunology},

volume = {164},

pages = {110427},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{cascarano_ultrastructural_2025,

title = {Ultrastructural Insights Into a textitCandidatus Parilichlamydia sp. Infection of Gill Goblet Cells in Greater Amberjack},

author = {Maria Chiara Cascarano and Papadogiorgaki Sevasti and Pantelis Katharios},

url = {https://onlinelibrary.wiley.com/doi/10.1111/jfd.14146

/wp-content/uploads/2025/05/2025-Cascarano-JFD-25.pdf},

doi = {10.1111/jfd.14146},

issn = {0140-7775, 1365-2761},

year  = {2025},

date = {2025-05-28},

urldate = {2025-05-28},

journal = {Journal of Fish Diseases},

pages = {e14146},

abstract = {ABSTRACT 

 

 Despite recent genomic studies and increased molecular data, epitheliocystis remains an enigmatic fish disease with no experimental in vitro or in vivo models to aid the advancement of research. In this study, we revert to a classical microscopical approach and screen with the electron microscope the epitheliocystis lesions caused by a 

 Ca 

 . Parilichlamydia sp., infecting mucus cells in Greater amberjack. We report distinct morphological features of this bacterial family, characterised by Intermediate Bodies that closely resemble those of previously described 

 Candidatus similchlamydia 

 , and Elementary Bodies that exhibit morphological similarities to 

 

 Chlamydia trachomatis 

 

 . We describe the characteristics of a novel Chlamydial Inclusion Membrane (IM) type, with abundant interdigitations, possibly shaped by fusion of the IM with cytoplasmic vesicles, and moreover discuss the presence of multivesicular bodies in the infected cell. Our observation of immune cells in the infected areas indicates an interaction of macrophages with infected cells, a role for granular cells as pathogens reservoirs and an active phagoptosis process in the nearby areas, overall shedding light on cellular immune processes characterising these infections in fish hosts.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{siaperas_multi-omics_2025,

title = {Multi-omics insights into the response of Aspergillus parasiticus to long-chain alkanes in relation to polyethylene modification},

author = {Romanos Siaperas and George Taxeidis and Anastasia Gioti and Efstratios Nikolaivits and Evangelos Topakas},

url = {https://linkinghub.elsevier.com/retrieve/pii/S0269749125007596

/wp-content/uploads/2025/05/2025-Siaperas-EnvPol-24.pdf},

doi = {10.1016/j.envpol.2025.126386},

issn = {02697491},

year  = {2025},

date = {2025-05-22},

urldate = {2025-05-22},

journal = {Environmental Pollution},

volume = {376},

pages = {126386},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{diaz-avello_aptamer-antibody_2025,

title = {Aptamer-antibody sandwich lateral flow test for rapid visual detection of tetrodotoxin in pufferfish},

author = {Ulises G Díaz-Avello and Vasso Skouridou and Xhensila Shkembi and Jaume Reverté and Manolis Mandalakis and Panagiota Peristeraki and Mònica Campàs and Ciara K O'Sullivan},

url = {https://linkinghub.elsevier.com/retrieve/pii/S0048969725010563

/wp-content/uploads/2025/05/2025-Díaz-Avello-TTX_LFA-test-DRAFT-SUBMITTED-23.pdf},

doi = {10.1016/j.scitotenv.2025.179419},

issn = {00489697},

year  = {2025},

date = {2025-05-20},

urldate = {2025-05-20},

journal = {Science of The Total Environment},

volume = {978},

pages = {179419},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{ventriglia_spermatogenesis_2025,

title = {Spermatogenesis advancement in pre-pubertal meagre Argyrosomus regius treated with recombinant gonadotropins},

author = {Gianluca Ventriglia and Neil Duncan and Ignacio Giménez and Constantinos C Mylonas and Chrysovalentinos Pousis and Aldo Corriero and Rosa Zupa},

url = {https://www.nature.com/articles/s41598-025-99372-3

/wp-content/uploads/2025/05/2025-Ventriglia-SciRep-22.pdf},

doi = {10.1038/s41598-025-99372-3},

issn = {2045-2322},

year  = {2025},

date = {2025-05-13},

urldate = {2025-05-13},

journal = {Scientific Reports},

volume = {15},

number = {1},

pages = {15113},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{papadaki_exploring_2025,

title = {Exploring the Fanconi Anemia Gene Expression and Regulation by MicroRNAs in Gilthead Seabream (Sparus aurata) at Different Gonadal Development Stages},

author = {Maria Papadaki and Ngoc-Son Le and Constantinos C Mylonas and Elena Sarropoulou},

url = {https://link.springer.com/10.1007/s10126-025-10444-x

/wp-content/uploads/2025/05/2025-Papadaki-MarBioteck-21.pdf},

doi = {10.1007/s10126-025-10444-x},

issn = {1436-2228, 1436-2236},

year  = {2025},

date = {2025-05-06},

urldate = {2025-05-06},

journal = {Marine Biotechnology},

volume = {27},

number = {2},

pages = {74},

abstract = {Abstract 

 

 Fanconi anaemia (FA) is a rare autosomal recessive disease in humans that is distributed worldwide. Fanconi anemia complementation (Fanc) proteins are essential for the appropriate functioning of the FA DNA repair pathway. They are also linked to a number of other biological processes, including oxygen metabolism, cell cycle regulation, haematopoiesis and apoptosis. So far, little research has been conducted on teleosts, but evidence shows that Fanc proteins play a significant role in immune response and sex reversal. For the examination of the expression of three 

 fanc 

 genes ( 

 fancc 

 , 

 fancl, 

 and 

 fancd2 

 ), as well as the potential regulation of these genes by microRNAs (miRNAs) in gonadal tissues at different stages of development, the present study has selected the gilthead seabream ( 

 Sparus aurata 

 ), a significant aquaculture species that exhibits protandrous hermaphroditism. The obtained data suggested the role of 

 fancl 

 and 

 fancd2 

 in the maturation of female gonads and the miRNAs miR-210, miR-217 and miR-10926 have been identified as putative regulators of 

 fancd2 

 , 

 fancc 

 and 

 fancl 

 , respectively. Overall, the data indicated the potential use of 

 fancl 

 and 

 fancd2 

 genes as sex biomarkers in conjunction with their respective regulation by miRNAs. To the best of our knowledge, this is the first study demonstrating the importance of 

 fanc 

 genes, along with putative regulatory miRNAs, in the reproduction of an important marine aquaculture species.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{thieme_development_2025,

title = {Development of convergent adaptations reveal highly conserved early ontogenetic skull shape in fishes with amphibious vision},

author = {Philipp Thieme and Vivian Fischbach and Ioannis Papadakis and Timo Moritz},

url = {https://www.nature.com/articles/s41598-025-91563-2

/wp-content/uploads/2025/04/2025-Thieme-SR-20.pdf},

doi = {10.1038/s41598-025-91563-2},

issn = {2045-2322},

year  = {2025},

date = {2025-04-28},

urldate = {2025-04-28},

journal = {Scientific Reports},

volume = {15},

number = {1},

pages = {8411},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{kapelonis_flying_2025,

title = {Flying Robots Teach Floating Robots—A Machine Learning Approach for Marine Habitat Mapping Based on Combined Datasets},

author = {Zacharias Kapelonis and Georgios Chatzigeorgiou and Manolis Ntoumas and Panos Grigoriou and Manos Pettas and Spyros Michelinakis and Ricardo Correia and Catarina Rasquilha Lemos and Luis Menezes Pinheiro and Caio Lomba and João Fortuna and Rui Loureiro and André Santos and Eva Chatzinikolaou},

url = {https://www.mdpi.com/2077-1312/13/3/611

/wp-content/uploads/2025/04/2025-Kapelonis-Mar-Sci-Eng-19.pdf},

doi = {10.3390/jmse13030611},

issn = {2077-1312},

year  = {2025},

date = {2025-04-11},

urldate = {2025-04-11},

journal = {Journal of Marine Science and Engineering},

volume = {13},

number = {3},

pages = {611},

abstract = {Unmanned aerial and autonomous surface vehicles (UAVs and ASVs, respectively) are two emerging technologies for the mapping of coastal and marine environments. Using UAV photogrammetry, the sea-bottom composition can be resolved with very high fidelity in shallow waters. At greater depths, acoustic methodologies have far better propagation properties compared to optics; therefore, ASVs equipped with multibeam echosounders (MBES) are better-suited for mapping applications in deeper waters. In this work, a sea-bottom classification methodology is presented for mapping the protected habitat of Mediterranean seagrass Posidonia oceanica (habitat code 1120) in a coastal subregion of Heraklion (Crete, Greece). The methodology implements a machine learning scheme, where knowledge obtained from UAV imagery is embedded (through training) into a classifier that utilizes acoustic backscatter intensity and features derived from the MBES data provided by an ASV. Accuracy and precision scores of greater than 85% compared with visual census ground-truth data for both optical and acoustic classifiers indicate that this hybrid mapping approach is promising to mitigate the depth-induced bias in UAV-only models. The latter is especially interesting in cases where the studied habitat boundaries extend beyond depths that can be studied via aerial devices’ optics, as is the case with P. oceanica meadows.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{mukiibi_integrated_2025,

title = {Integrated functional genomic analysis identifies regulatory variants underlying a major QTL for disease resistance in European sea bass},

author = {Robert Mukiibi and Serena Ferraresso and Rafaella Franch and Luca Peruzza and Giulia Dalla Rovere and Massimiliano Babbucci and Daniela Bertotto and Anna Toffan and Francesco Pascoli and Sara Faggion and Carolina Peñaloza and Costas S Tsigenopoulos and Ross D Houston and Luca Bargelloni and Diego Robledo},

url = {https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-025-02180-4

/wp-content/uploads/2025/04/2025-Mukiibi-BMC-Biology-18.pdf},

doi = {10.1186/s12915-025-02180-4},

issn = {1741-7007},

year  = {2025},

date = {2025-04-07},

urldate = {2025-04-07},

journal = {BMC Biology},

volume = {23},

number = {1},

pages = {75},

abstract = {Abstract 

 

 Background 

 Viral nervous necrosis (VNN) is an important viral disease threatening global aquaculture sustainability and affecting over 50 farmed and ecologically important fish species. A major QTL for resistance to VNN has been previously detected in European sea bass, but the underlying causal gene(s) and mutation(s) remain unknown. To identify the mechanisms and genetic factors underpinning resistance to VNN in European sea bass, we employed integrative analyses of multiple functional genomics assays in European sea bass. 

 

 

 Results 

 

 The estimated heritability of VNN resistance was high ( 

 h 

 2 

  textasciitilde 0.40), and a major QTL explaining up to 38% of the genetic variance of the trait was confirmed on chromosome 3, with individuals with the resistant QTL genotype showing a 90% survivability against a VNN outbreak. Whole-genome resequencing analyses narrowed the location of this QTL to a small region containing 4 copies of interferon alpha inducible protein 27-like 2A ( 

 IFI27L2A 

 ) genes, and one copy of the interferon alpha inducible protein 27-like 2 ( 

 IFI27L2 

 ) gene. RNA sequencing revealed a clear association between the QTL genotype and the expression of two of the 

 IFI27L2A 

 genes, and the 

 IFI27L2 

 gene. Integration with chromatin accessibility and histone modification data pinpointed two SNPs in active regulatory regions of two of these genes ( 

 IFI27L2A 

 and 

 IFI27L2 

 ), and transcription factor binding site gains for the resistant alleles were predicted. These alleles, particularly the SNP variant CHR3:10,077,301, exhibited higher frequencies (0.55 to 0.77) in Eastern Mediterranean Sea bass populations, which show considerably higher levels of resistance to VNN, as compared to susceptible West Mediterranean and Atlantic populations (0.15–0.25). 

 

 

 

 Conclusions 

 

 The SNP variant CHR3:10,077,301, through modulation of 

 IFI27L2 

 and 

 IFI27L2A 

 genes, is likely the causative mutation underlying resistance to VNN in European sea bass. This is one of the first causative mutations discovered for disease resistance traits in fish and paves the way for marker-assisted selection as well as biotechnological approaches to enhance resistance to VNN in European sea bass and other susceptible species.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{wei_dietary_2025,

title = {Dietary iron oxide (Fe2O3) nanoparticles modulate growth performance, body composition, mineral content and intestinal health of yellow catfish juveniles (Pelteobagrus fulvidraco)},

author = {Xiao-Lei Wei and Zhi-Wei Hao and Yannis P Kotzamanis and Tian-Hua Zhang and Zhi-Bo Liu and Hong Yang and Zhi Luo},

url = {https://linkinghub.elsevier.com/retrieve/pii/S2352513425001255

/wp-content/uploads/2025/03/2025-Wei-AquaReports-16.pdf},

doi = {10.1016/j.aqrep.2025.102739},

issn = {23525134},

year  = {2025},

date = {2025-03-31},

urldate = {2025-03-31},

journal = {Aquaculture Reports},

volume = {42},

pages = {102739},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{antoniou_comparative_2025,

title = {Comparative phylogeography of four lizard taxa within an oceanic island},

author = {Theodora Antoniou and Andreas C Dimitriou and Emmanouela Karameta and Aglaia Antoniou and Nikos Poulakakis and Spyros Sfenthourakis},

url = {https://linkinghub.elsevier.com/retrieve/pii/S1055790325000120

/wp-content/uploads/2025/03/2025-Antoniou-CompPhylo-pre-print-15.pdf},

doi = {10.1016/j.ympev.2025.108295},

issn = {10557903},

year  = {2025},

date = {2025-03-24},

urldate = {2025-03-24},

journal = {Molecular Phylogenetics and Evolution},

volume = {205},

pages = {108295},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{chalmoukis_development_2025,

title = {Development and Characterization of Fluorescent Protein-Tagged Vibrio harveyi Strains as a Versatile Tool for Studying Infection Dynamics and Strain Interactions},

author = {Charalampos Chalmoukis and Stavros Droubogiannis and Vassiliki A Michalopoulou and Adriana Triga and Panagiotis F Sarris and Pantelis Katharios},

url = {https://www.mdpi.com/2076-0817/14/3/247

/wp-content/uploads/2025/03/2025-Chalmoukis-pathogens-13.pdf},

doi = {10.3390/pathogens14030247},

issn = {2076-0817},

year  = {2025},

date = {2025-03-18},

urldate = {2025-03-18},

journal = {Pathogens},

volume = {14},

number = {3},

pages = {247},

abstract = {Fluorescent protein-tagged bacterial strains are widely used tools for studying host-pathogen interactions and microbial dynamics. In this study, we developed and characterized Vibrio harveyi strains genetically modified to express green fluorescent protein (GFP) and red fluorescent protein (RFP). These strains were constructed using triparental mating and evaluated for phenotypic, genomic, and virulence attributes. Genomic analyses revealed strain-specific variations, including mutations in key regulatory and metabolic genes, such as luxO and transketolase. While plasmid acquisition imposed metabolic costs, resulting in altered growth and antibiotic sensitivities in certain transconjugants, others demonstrated robust phenotypic stability. Virulence assays using gilthead seabream larvae revealed that most tagged strains retained moderate pathogenicity, with visualization of co-infections highlighting the potential for studying strain-specific interactions. Furthermore, fluorescent microscopy confirmed the successful colonization and localization of tagged bacteria within host tissues. These findings underscore the utility of GFP- and RFP-tagged Vibrio harveyi as versatile tools for infection dynamics, offering a foundation for future research on strain interactions and pathogen-host relationships.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{di_costanzo_high-quality_2025,

title = {High-quality genome assembly and annotation of Thalassiosira rotula (synonym of Thalassiosira gravida)},

author = {F Di Costanzo and M Di Marsico and I Orefice and J B Kristoffersen and P Kasapidis and T Chaumier and L Ambrosino and M Miralto and R Aiese Cigliano and F Verret and L Tirichine and M Trindade and L Van Zyl and V Di Dato and G Romano},

url = {https://www.nature.com/articles/s41597-025-04634-4

/wp-content/uploads/2025/03/2025-Costanzo-SciData-12.pdf},

doi = {10.1038/s41597-025-04634-4},

issn = {2052-4463},

year  = {2025},

date = {2025-03-14},

urldate = {2025-03-14},

journal = {Scientific Data},

volume = {12},

number = {1},

pages = {310},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{sarropoulou_circulating_2025,

title = {Circulating miRNAs involved in the immune response of the European seabass (Dicentrarchus labrax)},

author = {Elena Sarropoulou and Pantelis Katharios and Elisavet Kaitetzidou and Giuseppe Scapigliati and Andrea Miccoli},

url = {https://linkinghub.elsevier.com/retrieve/pii/S1050464825001214

/wp-content/uploads/2025/03/2025-Sarropoulou-FishShelImm-11.pdf},

doi = {10.1016/j.fsi.2025.110232},

issn = {10504648},

year  = {2025},

date = {2025-03-10},

urldate = {2025-03-11},

journal = {Fish & Shellfish Immunology},

volume = {160},

pages = {110232},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{katharios_protozoan_2025,

title = {Protozoan Co‐Infection Drives Fish Mortality Event in Crete's Karteros River},

author = {Pantelis Katharios and Evangelia Karageorgou},

url = {https://onlinelibrary.wiley.com/doi/10.1111/jfd.14099

/wp-content/uploads/2025/03/2025-Katharios-JFD-10.pdf},

doi = {10.1111/jfd.14099},

issn = {0140-7775, 1365-2761},

year  = {2025},

date = {2025-03-07},

urldate = {2025-03-10},

journal = {Journal of Fish Diseases},

pages = {e14099},

abstract = {ABSTRACT 

 

 In August 2024, a significant fish kill involving hundreds of flathead grey mullet (\textit{Mugil cephalus} ) was reported in the delta region of the Karteros River, Crete, Greece. The investigation identified the primary cause of mortality as severe parasitic infections, specifically from the protozoan parasites Amyloodinium ocellatum and Trichodina sp., both of which heavily affected the gills of the fish. Concurrently, poor water quality in the area, likely due to reduced water volume and limited water renewal, created favourable conditions for parasite proliferation while weakening the fish's natural defences. This event raised concerns due to the location of the fish kill in the Karteros River delta, a highly protected area designated as a small island wetland.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}